LOCUS       DAOMPG010000001        40133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_447_length_40133_cov_4.254005, whole genome shotgun sequence.
ACCESSION   DAOMPG010000001 DAOMPG010000000
VERSION     DAOMPG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 40133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_40133_cov_4.254005"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLS34_00005"
     CDS             <1..517
                     /locus_tag="PLS34_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRP85883.1"
                     /translation="KDKPIINVPGCPPVPTVITGVLAHYLTFGTIPELDRHGRPMAFF
                     GQNIHDRCYRRPFYDRGLFANTFDDEGARKGYCLYKLGCKGPVTYNACATLKWNEGVS
                     WPVQSGHGCIGCSEPDFWDKGSFYRPLSAAYWGDAKTVGIGAAAGAAAGLAAAALARG
                     KQKKSQQEEKA"
     gene            514..1194
                     /locus_tag="PLS34_00010"
     CDS             514..1194
                     /locus_tag="PLS34_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRP85884.1"
                     /translation="MNEGLYLQGMTLLDFARGPAFRWAVVIFVVGVVWRLTALLLASR
                     KPLEPAKGSATAGGVKTLLTRSAPAHELEKNIVFQHYSGYAWHISMFIVLLFFAPHML
                     FFKDILGFGWPTLPNLFITFFTVVTMGILLVLLARRLMNPVLRQISTMDDYISWIIAF
                     LPFLTGIMSYTHITFGMSYETVLGLHILSICLLLVWFPFGKLMHALFIWPSRYKVGAA
                     FARRGVRA"
     gene            1191..2516
                     /locus_tag="PLS34_00015"
     CDS             1191..2516
                     /locus_tag="PLS34_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRP85885.1"
                     /translation="MSATTIDYGTLGERAAAGVKPRALSDEQKIARAKEAFLAGMDAD
                     MATYLESCIHCGMCAEACHFYLATEDAKYTPIYKLEPLKKVYRRELAPTRWLNRLTTR
                     DITVADLEQWQELVYDSCTQCGRCSLLCPMGIHIQSMIQVVRNGLAAAGLAPAELQAV
                     SDEQKDKASILGAGPDKLREIVERVRAKGIDVPLDKDKAEVLVLSSALDFLKDESVLA
                     ATAKIMNKLGASWTIRSDGFESANFGALAGDKQAQKRISKRVIDAAIAAGAKKVIVAE
                     CGHAYPVLRWEAATLYGKELPFEFLSVAEYLGQELKAGRLKLKKAGNGAAHVTFHDPC
                     KIGRMGGSFDESRDVLKALGVKLTEMESHGRTNLCCGGGGGVAMLKSAQTLRRKAFEL
                     KREEVDRTGAPTVVTACNTCMQNLEMGKAWLNWDKSVVNLVQLVSDNLA"
     gene            2566..4299
                     /locus_tag="PLS34_00020"
     CDS             2566..4299
                     /locus_tag="PLS34_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRP85886.1"
                     /translation="MSNRVVVDPITRIEGHLRVEVQMQGDRIQQAYSSGTMVRGIEII
                     MRGRDPRDAWAFTQRICGVCTLVHGMASIRAVEDALKYEIPHNAQLIRNLMIGSQYVL
                     DHVMHFYHLHALDWVDVVSALSADAAATADLAKAVSPQWPKSTEGYFRDVKKKLQGFV
                     EGGQLGIFANAYWGHPAYKLPPEANLMAVAHYLEALAWQRDVAKLHAIFGGKNPHPNF
                     VVGGVPSPIDLNSDSAINAERLQVVQKIIDMMRVFVDQVYVPDTLAIAGFYKDWFGRG
                     EGLGNFISFGDLPSRSMTDSSSFLIPQGVILNRNLNDIQEVDFADPNEIQEFASHSWY
                     NYSGGKNQGLHPWDGETDLNYEGRGGPAAPYKQLNVDDGYSWLKSPRWKGHSMEVGPL
                     ARVLIMYAKGHDQTRELVNYALSTLDAPVEALFSTLGRTAARTLETKIIADAMQGWMD
                     QLLGNIRAGDTRTFNEIQWNPSSWPKVAKGVGIHEAPRGGLSHWIVIKDGRIDNYQCV
                     VPSTWNAGPRDPQGVPGAYEAALQDNHQLYDPEQPIEILRTIHSFDPCLACAVHVTDP
                     DGKELIKVKAL"
     gene            4425..5744
                     /locus_tag="PLS34_00025"
     CDS             4425..5744
                     /locus_tag="PLS34_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP85887.1"
                     /translation="MDEFFTPQQVIDVANSAWAWARAQVFALPNLLQLLTVAVGLAAA
                     ALLRPRLQRLIERLAWGARWKRAVDMLRSLLLPLLWLVLVGIAWQAAVIQAWPARILE
                     IAASLLMAWVVINIASSLIRNPVWARAVAITAWTIAALNILRLLDPTIALLDSFAIQF
                     GAVRISLLTVTKSVFALAVLLWLATVVSETLESRLRHSPNLTPSVQVLFGKLLKFSLV
                     TIAVLAAIATTGMDMTALAVFGGAIGVGIGLGLQRVIANLFSGVILLLDKSIKPGDVV
                     AVAGTYGWVNTLGGRYVSVVSRDGIEHLIPNEEFITTRVENWTHTDSKIRVKIPIGVH
                     YQSDVEHALAICVQCAEQVPRVLADPAPVCQLKSFGDNSVDLELRIWVGDPMNGVNNV
                     RSEILRLVWRAFHEHGIEIPYPQRDLHVRAGEPLQVVVAAAAAERST"
     gene            5741..7708
                     /locus_tag="PLS34_00030"
     CDS             5741..7708
                     /locus_tag="PLS34_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRP85888.1"
                     /translation="MSERQGLGPGAFEPPRPGTSYEPYVPAHASLQEFTFKAIGLGIA
                     FGIIFGAANAYLGLRAGLTISTSIPVAVMTVAVFRALAAVGRSGTLLEANLSQTVGSA
                     SSSLASGIIFTLPALFLWGLSPSLLQMTMLAMCGGVLGVLFMIPLRRFLIEREHGKLP
                     YPEGTACAEVLVANEVGGGNARFVFIGLAAGAAFKALTSWARAIPDAVHLKLPWPRKA
                     ELGMDLSPALFGVGFILGPRIAAVMVGGGLLSWLVVIPLLDWWGASRSLPLYPETVLL
                     MTDMSPSQLWSRYVRYIGAGAVATAGIITLVRSLPVMVDSFRIGFGQLQKQRAEHPVA
                     VPRTGQDLPLKVVGIGAVLVIATLGLVPVPFGEVGGVVHRLVAAVCVAVFAFFFVTVA
                     SRIVGLVGVTSNPTSGMTIAALLGTSGIFLAMGWTDMTGKAAAITVGCVVAIAASIAG
                     DTSQDLKTGYLLGATPRRQQVGELVGVLTAAVFVCLAVLLLAETFGFGSTELPAPQAT
                     LMKLVIDGVLDQSLPWGLVALGIGIAVACELLKIPSLPFAVGVYLPVSTMTPIFLGGL
                     LRLWAERSATGTDELNERRERGILLGSGFVGGEGLLGVGIAGAAFVLGRRPLGIGTGW
                     LGGDAVAALVGLVAFTAFAAWFYLQVRRRAD"
     gene            7698..9332
                     /locus_tag="PLS34_00035"
     CDS             7698..9332
                     /locus_tag="PLS34_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRP85889.1"
                     /translation="MRTEDAGKGRLGRWTTLAVAVLVVLAGLGPAAAAPDPVEVAETI
                     RRSVEQLRSTGEVTVAGRKLRSARTLPEVYEARGFTLLWRDRVSEEALLGEIAAISGE
                     GLDPGDYHFEALRAALDRRRQQADSAAAAATADLLLTDALLRVAAHLHQGKVDPATGS
                     PRWDLLGPVRGESGAALATRIASSGAVGLQLAELRPVQPMYGRLRSALARYRVIEQQG
                     GWEPLASGRVLQAGLEDPRVPALRRRLAATGDFTGIVVDSPRFDGALEEAVRRFQSRH
                     GLEPDGIFGPASLRVLNVPLKVRQDQLRANLERARWLLVDVRGRFLLADPAGRRVVLM
                     DNSEPVFRVAAEFSTAARAVPPFRAELRYLVANPDWVLTPGLVETQVAPLARRAPEQL
                     SARGLQVFNAAGDALDAAAVDWSAPGGVIVRQLPGERSFLGNLRFPIHNHAGVFLHGG
                     PARGEALAGSVRLADAAGLAHALAAGPTASSAESLAAALAAGTPRTLSLATPVPVLYG
                     SWTAWVQADGTVSFRAGYEDRDAAIIAALGRGADRR"
     gene            9431..10912
                     /locus_tag="PLS34_00040"
     CDS             9431..10912
                     /locus_tag="PLS34_00040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRP85890.1"
                     /translation="MEQLSGLDAAFVYLDSRNAPMHVGGLHVYQPESGRFDFEDFQAH
                     IAACIEGSPLFRRRLVQVPLHLGRPFWAEDPNFELDLHLQHSALPRPADWARLCELAA
                     RMFSRPLDPERPLWHLTFVSGLDACGVFPPGSFAVISRVHHAAADGLGTLDMFNAVWS
                     AEPRRRKRKTVGTRRAPVEPVPGALGLLKRSFGGVFRQPGETLSWAASLLRGGLGAGR
                     EFIARDHHAPPMLFQAPRSPFNVPISSRRCFDAVKLPLDEIRNIRAAVPGATVNDVVL
                     AVCSGGLRRYLQSYASLPEKDLVAMAPISIRGKMEKGSNRVSAMLIPLEVGARDPVER
                     LRRIHAHAVDSKAYTDAIGAATLADTSRFVPYSLATAAAGLYSGLHLARYHRPPFNLV
                     ITNVPGPREPLYLGTARLLEGFGTAPVLDGLGLILVVTSYMDTLSVSATAAHEAVPDV
                     ERLIESIRASFMELRDAVAARATPKAARGRASRPRVKRGQGAG"
     gene            10902..11672
                     /locus_tag="PLS34_00045"
     CDS             10902..11672
                     /locus_tag="PLS34_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP85891.1"
                     /translation="MQAEEELGPPPLIWQLLEGRAVIELGAMAWLYPLLRQAPAGDGH
                     PVLVLPGFLASGSSTFPLRQFLKSLGYAAHRWKLGRNLGGVGETEERLLARLRELRRR
                     YGRKVSVIGWSLGGMYARELSWLAPDDVRLVITLGTPFRHHTGTTVTLLYEDVSGQRE
                     ATMDPRLLARLAQPPPVPATSIYTRTDGVVHWRCSLEHPSPTTENVRVHGSHCGLGHN
                     PMALWAIADRLAQPEGDWQPFARHAFRRFAYPAPDRGD"
     gene            11675..13078
                     /locus_tag="PLS34_00050"
     CDS             11675..13078
                     /locus_tag="PLS34_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRP85892.1"
                     /translation="MQEFVDAANQLMWGSLLIWLLLGAGLLFTVLSGFIQVRHFGHTF
                     GVMKRSFRREHGGISSFEAYCTSLGARVGTGNLAGVAVALELGGPGAVFWMWVTAMLG
                     MATSFFESTLAQLFKVPNGDGSYRGGPAFYMQYGLGKRWMGILFSVFLIVSYPIAFNG
                     VQSNSMSAAVEQVTGIDAGWTGLLTAFLVGLVIFGGIRRIARVAEIVVPFMAIAYLGV
                     ALWLVLSHLDRLPGVVRLVFESAFGIGQAAGGVLGYGIAQALINGVRRGLFSNEAGSG
                     SAPIAAATADVRHPASQGYVQMLGVFTDTIVICSCTAAIILVSGQFEAGAGVTGMALT
                     QGAMSALVGDWGAGFMAVALLFFAFTSIMANTYYGETSLAFIRSDQRLLTAYRCVVLA
                     GIFAGSQIDLPLVWAMADLSMAFMTIVNLVAVLLLSGLVIKVMRDYNSQLALHVEPVF
                     DLARFPDIARRLPRGGW"
     gene            13101..13574
                     /locus_tag="PLS34_00055"
     CDS             13101..13574
                     /locus_tag="PLS34_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRP85893.1"
                     /translation="MNRFVRAMQSPEAQKLLLNCYPPYLMTGITVHRITPDWRRVEVS
                     MALRWYNRNYFGTHFGASLYGMTDPFYAIMLSRNIGPGYFVWDRSATIDFLRPGRGRV
                     SARFELTAEMIESVLAATASGEKHQPTWQVEVRDAAGELVAQVSKTLYVRRKPQR"
     gene            13639..14721
                     /locus_tag="PLS34_00060"
     CDS             13639..14721
                     /locus_tag="PLS34_00060"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRP85894.1"
                     /translation="MHARKLSLLLFAALGVAAATGIVRCIAHGPERVTTAALQSALAG
                     IDRLAAEFPSPGDAPLAFPADHAPKPGQFVESWLFAGLVRDDAGEAWGFQLAFERVAV
                     QAEAVSRESEWAVRDVWRARLAVERAGTRAAAGERISRAALGLAGASDTPAAAWVEDW
                     RFELQESGDRFRLQGAAGGAAVDLHLRLPATAPVAIGREPYRGYWWPGLEVAGTLVLE
                     GRESAVTGRAMLDRVWGRGLPVGRGQLALAGLWLELADGAAYRCEQLRRRAGGGTPLT
                     ECNTVPPADALLLAPRDDGWRVLDGVRYPLQWQVQLGAEVGTLGLRPLSASGAVWLDG
                     AWVGIVVAEGDREGWGLLQLSNFAPP"
     gene            14754..16157
                     /locus_tag="PLS34_00065"
     CDS             14754..16157
                     /locus_tag="PLS34_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP85895.1"
                     /translation="MKIHNIITGLALAGGLLAGCGRGAEVPLGSGEPGPLPAAQQRSG
                     DPALGYEILLNRGYVSCGVPLDAYLASAPDRSPEPALHGRVGRNAELPYDLNATIDTN
                     GVELVVSNCLACHGGRFNGELVIGLGNAFADFSSDPMPGIEGIGLLVEDGAPAQAWQR
                     WADRVAAIAPYMVTDTVGVNSAPNLTMALMAHRDPRTLRWHDAPRLEPPPREPLPTAV
                     PPWWRMQKKHAMFYHGGGQGDHVPYMMLKSLVCTDDLEEARMIDGWFTHVAAYIKSLE
                     PPRWPWPVDESLAGEGQKVFEAHCAACHGTYGERWTYPNLLVPLEQVGTDPAYALQAV
                     EAERFIRWFNGSFYGRGAQARPARGYVAPPLDAVWATAPYLHNDSVPSIAALLDSSRR
                     PTYWRHRTEPREYDQAALGWRYEALQHGKEGAGDADGARRIYDTTQRGYGNGGHLFGD
                     SLDEGQRRALIEYLKTL"
     gene            complement(16169..17023)
                     /locus_tag="PLS34_00070"
     CDS             complement(16169..17023)
                     /locus_tag="PLS34_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP85896.1"
                     /translation="MTEIFTAMPLLGRTLLILGVAVALHFGVHLVRVGGERLTRSEAT
                     ASVRKLRTTISLATSVAVFTMYFIAIGFILQEFGISMTAYLASASVIGLAVGFGSQGV
                     VQDVVTGLTLIFSNLIDMGEMVNIGGQTGIVRAIGMRFIVLENSHGALVNIPNRTVSN
                     VVSYPRGYVRLIVDVSLSDDEDVRARMIAAAERLLPTVAEQFPGIMRSPPSVEGLQTT
                     SAGRSFLRLKFRIWPDRVEPVGQSFRQELMHTLRKLDPGYADWMVAVHFEVERPRQRT
                     RNLRLLGR"
     gene            complement(17057..19774)
                     /locus_tag="PLS34_00075"
     CDS             complement(17057..19774)
                     /locus_tag="PLS34_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRP85897.1"
                     /translation="MEAPHTTVRSQAWHALDAGQALERLYSGAEGLNKSTAAERLAAH
                     GPNLLPPPPRRGPLRRLAAQFNNVLIYVLLVAAAITALLGEWADTGVILGVVVINSLI
                     GFIQEGKAERALEAISAMLSPRAMVIREGHKHEIAAEQLVPGDVVVLQAGDLVPADLR
                     LLKTRNLQIDEAILTGESVPVEKSTAPVARDAALGDRASMAWSGTLVTAGVGTGVVAA
                     TGGATEIGRISALLLRVQTLATPLTRQLERFGRLLSLVIGGVAAATMVVGVLLHDYAL
                     EDMFMAAVGLAVAAIPEGLPAIITITLAIGVQRMAARNAIIRRLPAVETLGSVSVICS
                     DKTGTLTRNEMTASRVVLQSREYQVTGVGYDPLGVIQSGAEEADPSTDKALMELCRAA
                     LLCNDAEFEQGAQGSRLRGDPTEGALLVLAMKAGLEPDRLRPSAPRDDAIPFESERRF
                     MATLHHDHDGARFIYVKGAPERILSMCVDALSDHGAMPLDRERWLASAERLAVDGLRV
                     LALARRTPPNGRRGELKTSELEDSLSLLGLVGLIDPPRDEAIKAVAECRSAGIRVKMI
                     TGDHLSTARAVGQAMGIGDGQGAVLGEELQRLDDARLAEVVQRTDVFARSSPEHKLRI
                     VEALQAHGHVVAMTGDGVNDAPALKRADVGVAMGRGGTEVARQAAEMVLSDDNFASIE
                     CAVEEGRTVYDNIRKAILFILPTNAAEAMMILLAIVLGRSLPITPLQILWVNMVTAVT
                     LALALAFEPPEQDIMQRPPRHPDEPLLGRFVIWRISFVTMIVVAGTFGLFVEARAAGY
                     ELETARTLAVNALVLFEAFYLLNVRHILAPTLTLNGLFGSRIAIGAVMAVFGLQLLFT
                     YLPAMNALFGTAPLDLGDWLLVVLVAAVVVPLVELEKFVLRRRAATLRG"
     gene            19964..21733
                     /locus_tag="PLS34_00080"
     CDS             19964..21733
                     /locus_tag="PLS34_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP85898.1"
                     /translation="MPVKTPRSRDSLAVAGLALLLAVLQPSPAAAQENGEPAAELERV
                     VVVSTRSPRPVSEVVGMVTILDDSDVAAIMATNEDGLWRYTPGIQVESSGSRYPAHSL
                     SIRGIGGNRVLMELDGIPIQERFLVGNFADAGRSSSELDFIRRVEVLRGPASSLYGSR
                     AIGGVVAVSTFDPDDLALGPGAPGALVRGSFASDWDAFGASAIRGWQHESVGLLLGAS
                     HRHGNEPDRSANPDSPDRLDRDRQAMLAKLTLRDDEDNGLRLTLDGDRVDTAAEINSF
                     IGQGRFANTTGLFGDDRSTRTGIALDGHMAHREYLVEGIVFHRETQTRQDTVDLREAL
                     ARPVRIDRGFRYDTTISGVRTRVSRQFEHGGLRHRVMLGAEFSRSELDEMRDAALTGI
                     LDGQTTNVVLGESFPLRDFPVTESRETGVFLQDEIDWLSGRWTAIPSLRFDRTRIRIR
                     EDDAWRRANPQADAAELTVSDVSPRLGVLWRPSRDWQAWGQVASGFRAPPAEDLNIGL
                     DIPLFRTRALPNPDLKSETSLGWEVGLRTCGRAPATPTRRWPRSGPTTRTSSSRWCRS
                     GRTRPPGSCCSSRRTGIVRGSGG"
     gene            21652..22200
                     /locus_tag="PLS34_00085"
     CDS             21652..22200
                     /locus_tag="PLS34_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP85899.1"
                     /translation="MPLGPDPATGILLFQSQNRDRARIRGIEVEGGMPLGQLTPALDA
                     FAAGVSAYWAEGEDRRTGEDLVDVGPPSAVLFVDWASPSAAWEIRLSGTFARGKQGDD
                     PAGTPDFSVPGHGIVDLVTAWRASERLTLRAGLFNVTDRTWWRWGDTRGLPADDPLIP
                     VRSAPGRSITVSFNFGFGPGQY"
     gene            22241..24397
                     /locus_tag="PLS34_00090"
     CDS             22241..24397
                     /locus_tag="PLS34_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRP85900.1"
                     /translation="MNSRVLMITLLAGLAMAGCGPREPQQAAPPRTGQDPATAVHAAE
                     VASAGQSLEQRMTAGRKVYQNVCQACHGPEGKGLPGAFPPVANSDWIEQNPDQLIGAI
                     LNGLSGPIVVNGQNYNAVMPPMAYLSDEDVANVLTFVLNSWDNPGGEIQPAQVAQVRG
                     GAPAAATTAPGEHPVPGTSALKYEGAPLGMDPETARRLVDSEGPPITHAEWARSTELY
                     FERCAGCHGVLRHGATGKPLTPDVTREKGTEYLKALITYGSPAGMPNWGTSGDLTEGE
                     IQAMAHFLQHEPPAPPEFGMEEMRATWKVLVPPEARPDKPQHNRNIDNFFAVTLRDSG
                     EIAIIDGDTKEIVAVVPTGYAVHITRPSASGRYAITIGRDARIDMIDLWADPPARVAE
                     IKIGLEARSVETSKYKGYEDRYAIAGAYWPPQYVVMDGDTLEPLKIVSTRGMTVDTQE
                     YHPEPRVAAIVSSHEHPEFIVNVKETGQILLVDYSNVNALKTTTIGAARFLHDGGWDA
                     SKRYFLTAANQSDKIAIVDAREQKLEALVDVERIPHPGRGANVIDPKFGPVWITSALG
                     NADISFIATDPAKHPEHAWKVVRVLQGQGGGSLFVKTHPKSRNLWVDTPLHPEEKVSQ
                     SIAVFNIDDFEAGYEVLPIADWANLGPGPKRVVQPEYNKAGDEVWFSVWNTMDARSAI
                     VVVDDKTRKLKHVIDDARIVTPTGKFNIYNTMHDVY"
     gene            24516..>24921
                     /locus_tag="PLS34_00095"
     CDS             24516..>24921
                     /locus_tag="PLS34_00095"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRP85901.1"
                     /translation="MKRRTVIGHVAAAAILPAFFPLVMRSSAASEAAPGDLRQVSHER
                     WRELLPADRYRVLFEEGTEPARSSPLDKRYDDGTYICAACYQPLFDSADKFDSGTGWP
                     SFTRPLEGAMGTKRDFRLIWPRTEYHCSRCGGH"
     assembly_gap    24922..24931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24932..25040
                     /locus_tag="PLS34_00100"
     CDS             <24932..25040
                     /locus_tag="PLS34_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRP85902.1"
                     /translation="FNDGPPPTGQRWCNNGLALEFVPRGEPLPPLRDPS"
     gene            25037..25708
                     /gene="msrA"
                     /locus_tag="PLS34_00105"
     CDS             25037..25708
                     /gene="msrA"
                     /locus_tag="PLS34_00105"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRP85903.1"
                     /translation="MSARGACPAAPLASLCLLFLGLAAHPDAAADEGGGGEGRVTGEA
                     GLALATFAGGCFWCMEPPFDALEGVVSTTAGYAGGAERDPTYRQVASGQTGHAEVVQV
                     TFDPSRVSYAELLEVFWRNIDPFAVDRQFCDSGPQYRSAIFHHDELQRAAAEASLAAI
                     EARLGAAVATRIEAVGDTFHAAEEYHQDYYLKNPLRYRYYRYNCGRDRRLEQVWGSES
                     SREDK"
     gene            25929..27086
                     /locus_tag="PLS34_00110"
     CDS             25929..27086
                     /locus_tag="PLS34_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP85904.1"
                     /translation="MCASRDSRCSPIAASAALLALLVGLTGCGRPQDGSTGHGQGGGM
                     PPAPVTFMTAAAETVQIEQDYTGRLHGWREAEVRARVGGILEERLYEEGAQVEEGAPL
                     FRIERQAYRIALQRAEAELANAQAALNQAQREWRRVAGLHEQRAVSDRERDQALSARE
                     LAEARLALARAGVSQAQLELEYTTVLAPVGGVSGPEAVTPGNLLAPGALLTTVTQLDP
                     IQLRFSLPVADAEARRRLAGDSAESTADVRLVLPDGTVYEERGHIDFTAGTVDARTGS
                     VNVRAVFPNPAGSLAPGALARVRFPVERLESVHLVDSVAVLQGPAGPVVFVVDEENTA
                     RARPVKLGPMIGNRRAVLDGLADGDRVVVNGQVALRDGAPVQPQPRDGQAD"
     gene            27086..30217
                     /locus_tag="PLS34_00115"
     CDS             27086..30217
                     /locus_tag="PLS34_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRP85905.1"
                     /translation="MLRFFIDRPIFSAVISIVIVIGGAAALRALPVEQYPDVVPPQVV
                     VTAVYPGASAQVLADSVAAPLEQEINGVPGMIYMQSSSTDSGAVQITVSFEIGTDPDQ
                     AQINVNNRVQAALSRLPQRVRDLGVRVEARSTNILLVAVMISPDGRFDTLAISNYALL
                     NVLDELVRIPGVGDASLFGAQDYSMRVWLQPDALAAYQLTPSDVAAAVREQNAQFAAG
                     SIGAEPAPRGQDFTFAVSTRGQLSTPEEFEQIILRSDADGGVLRLGDVARVELGARDY
                     AFAASYGGQPAVPMGVFLQPGANSLETADAVRAAFEEMSARFPEGLEYRIPYDTSVFV
                     DISIREVYVTLIVAVLLVVLVTFVFLQHLRATLIPVAAIPVSLIGTFLGMQAFGFSVN
                     LLTLFGLVLSIGIVVDNAIIVMENVERLMTEQAMKAREAAIETMQQVAGAVVSSTLVL
                     VAVFVPVTFLGGLSGELYRQFAVTIAISVVVSGIVALTLTPAMCALLLDKEARTVAAP
                     FRVFNRGFERVTGGFVAGVAFLLKHAVLGVVLFAGVIALAGVLLARLPSGLVPQEDQG
                     VALVVGLLPATSSLQRTEEVRDVLTSGLLEFEEIQEFTSFAGFDIIAGSLRTNAIVGF
                     AKLTDWREREGEGQDAASVTGRIMGLGFGIQEANVFAFTPPPIQGLSLTGGVEGYLQS
                     RGETSAGALESLVGRLRAAVAERPEIGSLQVPFDANVARYRADIDRDQVRALGVPINA
                     VFDAMQSTFGALYVNDFTLMGRNWQVNLQSEGEFRSRPEDLRRVYVRSAQGEMVPLSS
                     LVRLVRTGDTDAVDRYNLFVSARVLAEPAPGYTSGQVKAALEAAVEQALGTDARIGWI
                     GEAYQLEAAAGAGTAAFAMGLIMVFLILAAQYERWTLPLAVATAVPFGVLGAALAAML
                     RGYPNDIYLQVGLLVLIGLAAKNAILIVEFAAQNRAAGMSALEAATAAARQRFRAIMM
                     TALTFIIGSLPLVFATGAGAASRREIGTVVVGGMIAASTLALLFVPLFFKLLEDLSAW
                     RRQRRGGEEASHG"
     gene            30210..31625
                     /locus_tag="PLS34_00120"
     CDS             30210..31625
                     /locus_tag="PLS34_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRP85906.1"
                     /translation="MVKAAMVLLAAAALAGCAVGPDYQAPQLELPEHWPDNVVLDPAS
                     GELLAAWWRRFEDPVLDGLIERALADNLQVRMQAARVEEARARLGLARAEQWPTVGLQ
                     AEAARQRQPAATFGIAGLETPPRNLFSISGVLGYEIDLWGRLAREREAAMARLQGSLY
                     AQEAVRLGVIADVAVGYFNLRSAQRQLDVTERTLAARLEGVRIERLRFEAGQIDELAY
                     REAESQLAGTRAELPGRISELRRRESALGLLLGLSPEELLGALQVDAGTLGEITLPAG
                     IPAHLPSTLLVRRPDVRAAEAELAAATAGIGVAQAARLPQFNLAGLLGTAAGSAGDLF
                     GDEAQAWSIGTSIGVPLLDFGRGRTQVETAEAQRLQAEMAYRSTVANAFVEVRDALVF
                     YESSGARLAAIDAQVQALKRTEELAGIRYRDGYVSIIELLAAQRALLAAELALAQAEA
                     DRLAAAATLFKALGGGWEAKW"
     gene            31622..32245
                     /locus_tag="PLS34_00125"
     CDS             31622..32245
                     /locus_tag="PLS34_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRP85907.1"
                     /translation="MRRTREDAQVTREAILDAAQRVFLARGVSRTSLEQIAREAGVTR
                     GAIYWHFKDKSDLFSAMLERVRLPLSDLADAYRQGGGRRDPLGMLEKLCGLALTKLDA
                     SETYRKVYIILMTRCEFAGEMNPAFERQLEIDRDNLRKVEQDFVEARELGQLAPHVDP
                     RIATLTLYSLMHGIYQSWLRTPQSYDIRTDGAAMLRLFFTGLRATGH"
     gene            32299..33672
                     /locus_tag="PLS34_00130"
     CDS             32299..33672
                     /locus_tag="PLS34_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRP85908.1"
                     /translation="MLANLGLLISLGLLIWLVLRGVNVILASLLAAIMVALSNGLPLA
                     ESLLQHYATGRSGAFTFAGQFFLIFFAGAIFGKVMAEGRATTSIALLFRDLLGRERVL
                     WIVMLACALLTYGGVVVFVVIFAVYPLALDLARDANTPKRLLAGAAALGAGTFTMTAL
                     PGTPSIHNVIAARSLGTDLFAAPLLGVVAALLMMTLGMAYLERQRRRALASGEGFDPA
                     PHEHVPDDAEIEQGLPHWGVALLPLLVVLAIIIGPRLIGVFVTPGGESTLARLVAFAA
                     GQPVFWPSLALACGTALALLLLPRLGGQRMASLGRGTNEAILPIMNTAAIIGFGGVVI
                     QTIGFRQFSGLVVETGLPPLLSAFLSISTVAGITGSASGGLQIFMASLAPRYLEMGIE
                     PETLHRIATLASGGFDSLPHCGAVVVMMTIMGLSYRTAYRDIAIVTIVVPVLATLAAM
                     ALAGVAG"
     gene            complement(33680..34993)
                     /locus_tag="PLS34_00135"
     CDS             complement(33680..34993)
                     /locus_tag="PLS34_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP85909.1"
                     /translation="MNDHDHARFERLRWTIYGVLVAAYMLAFFHRFAPAMVSAELAQA
                     FGISAAALGSLAAMYFYIYTVMQIPAGVLADTLGSRFAVTLGNVVAGAGSVVFGLAET
                     FATASIGRFLVGLGVSVVFVGLMKSNTVWFSERRYGTISGLTLLLGNLGAVAATGPLA
                     LMLLHVDWRMLFVALGVFAMGLGLVSWWLVRDKPEDLGFPSVRQMEGRAAHAPRTRHW
                     VHDLRAVLANRRLWPGFAYDFGMTGSLFAMIGLWAVPLLRDVHGLSRGEASLYTTLAT
                     IAFALGCLAAGSLSDRMLRRQPVLRAGALVYFAVCLGLLFLPWRPGPFAFALFALLGL
                     SAGSFVVAYAHAKEVAAPALSGMAIALVNTGLFLGTALFQPLFGWAMDLGWDGRMLDG
                     VPVYAAGDYQRGLWLLAGFAGLAAIASRFLVETACRNISAEAPDT"
     gene            35183..35899
                     /locus_tag="PLS34_00140"
     CDS             35183..35899
                     /locus_tag="PLS34_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP85910.1"
                     /translation="MNDTTPGLLVVDDDEVVREMLRDYFSRQGFEVRLAGSAAEARSM
                     LDAGTPQLVFLDVGLPGEDGLSLARHIREKLDIPIVMISGAGEALDRIIGLEVGADDY
                     VTKPFDPRELLARVKSIMRRYQRAPEALEERPAERVRIPGFELDLASRRLFRADGEEL
                     ILTRMEFDLLQVFVEHPNRVLSRDQILNMTQNRDWDPFDRSIDIRVARLRRKLEDSPD
                     SPVLIRTVRGVGYMFVPDAA"
     gene            36057..38852
                     /locus_tag="PLS34_00145"
     CDS             36057..38852
                     /locus_tag="PLS34_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP85911.1"
                     /translation="MQPLPAEALRQLLDATSAIVAVKDLEGRYLHVNRAFLVLLGGEE
                     RDYLGHTDQQIFSPAMAAQLRANDQRVLAERRELVFEEDLEVDGRLHSYNTRKYPLLD
                     AEGRPWAVCLTAEDVTDARRTDQAMRRVALGISAASGTEVFELTVASVAAVLGVDLAF
                     VAAFEEDSGKRRLSALAMCEEGQVGGNYCYEIRGTPCEMVLAHGFEYYSENAAEMFTG
                     DPDLRAAGYVSYAGYPLMDADGEAAGVLSVCHRGPLPPVGMVQSVLAIFAVRTASELE
                     RVRMDRALRDSEQSYRTIFEASQDCIFIHDVDTGALVDVNPRVCETYGYEYDEILKLG
                     PGDLGSGEPPYTQADAEAAMRRARSGAVVKVEWHRRNADGTLAWDEVVLKRVRLAGVD
                     RIMAVTRDITERKEREEALSRSEDRLRATVEAALDCIITIDSGGRIREFNPAAERCFG
                     YRREDTLGRPLAELIVPPRLRQAHQRGMERYLMGGESRMLGRRVELTAMRADGSEFPV
                     ELAIAEAQGVDGVVFVGYLRDITLQREAERERAGLEAQLRQAQKMEAIGHLTGGVAHD
                     FNNILTGIMGYLGMADDIARELEHARLQQYLARARHSGTRAADLIQQMLTFSRGRRGD
                     ARAMAPAPLVADALRLVRATLPATVVLARKLDDDVPGIMFDAVQLEQILVNLCINARD
                     AMGGQGSLEVSLRGIEVEAAVCTSCQQAFRGRHVVLSVEDTGPGVPDEVLHRMFEPFF
                     TTKAPGQGSGMGLATVHGIVHEHGGHIVVANRSGGGAAFHVLLPPLDHAADPAGADPG
                     EGRAEPEAVAALSGRVLLVDDDETAREFMTDLLGEWGLAVTALAGPLEARAVDAGGAR
                     FDVAILDHTMPHMTGLELARRLHAEDRELPVLLYSGYAEQLAAGELAAAGIRAQLSKP
                     VDTAALFKHLETLLARR"
     gene            38997..>40133
                     /locus_tag="PLS34_00150"
     CDS             38997..>40133
                     /locus_tag="PLS34_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HRP85912.1"
                     /translation="MAIYQSVLDTVGRTPVVRLHRLAPEGVELYVKLESANPMGSVKD
                     RAALAIIEQAEADGTLQPGQTVVEATSGNTGISLAMVCARKGYPLVVVMAENFSVERR
                     RLMRFMGAQVVLTPAAEKGSGMLAKAVELARTHGWFLCRQFENEANADVHAHTTAQEI
                     LADFAASGLDYWVTGAGTGGTLSGVARALREHSPGTRVVVCEPDNAPMLGSGIPQPDS
                     AAPDRAASHPMFRPHLMQGWSPDFIPELTQRAITSGLVDEFQPVNGAEALATARLLAA
                     QEGILAGTSGGATLAGALALCKRVPAGSRVLCMLPDTGERYLSTPLFADVAADMDEAE
                     RLISASTPGFRFDLPAAQVPTAAAAPPETDAETERYVAGVFTDPQ"
BASE COUNT         6194 a        13395 c        14038 g         6496 t
ORIGIN      
        1 caaggacaag ccgatcatca acgtgccggg ctgcccgccg gttcccactg tgatcaccgg
       61 cgtgctggcg cattacctca ctttcggcac gatcccggaa cttgatcggc acggacggcc
      121 gatggccttc ttcggccaga acatccatga ccgctgctac cggcggccgt tctacgaccg
      181 cgggctgttc gcgaacacct tcgacgacga gggcgcgaga aagggctact gcctctacaa
      241 gctcggctgc aagggcccgg tgacctacaa cgcctgcgcc acgctgaagt ggaacgaggg
      301 cgtcagctgg ccggtccagt ccggacacgg ctgcatcggc tgctccgagc cggatttctg
      361 ggacaagggc agcttctacc ggccgctgtc ggcggcgtac tggggcgacg ccaagaccgt
      421 cggtatcggt gcggcggcag gcgccgcggc gggcctcgcc gctgcagccc tggcgcgcgg
      481 caagcagaag aagtcgcagc aggaggaaaa ggcatgaacg agggactgta tctgcagggc
      541 atgaccctgc tcgacttcgc ccgcggcccc gcgttccgct gggcggtcgt gatcttcgtg
      601 gtcggggtgg tgtggcgcct gaccgcgctg ttgctggctt cgcgcaagcc gctcgagcct
      661 gccaagggct cggccacggc cggcggcgtg aagacgctgc tgacgcgctc ggcgcccgcg
      721 cacgagctgg agaagaacat cgtcttccag cactactcgg gctacgcctg gcacatcagc
      781 atgttcatcg tgctgctgtt cttcgcgccg cacatgctgt tcttcaagga catcctcggc
      841 ttcggctggc cgacgctgcc caacctgttc atcaccttct tcaccgtggt gaccatgggc
      901 atcctgctgg tcctgctggc gcggcggctg atgaacccgg tgctgcggca gatctcgacc
      961 atggatgact acatcagctg gatcatcgcc ttcctgccgt tcctgacggg gatcatgtcg
     1021 tacacgcaca tcaccttcgg gatgagctac gagacggtgc tggggctgca cattctgagc
     1081 atctgcctgc tgctggtctg gttcccgttc ggcaagctga tgcatgcgtt gttcatctgg
     1141 ccgtcccgct acaaggtcgg ggccgccttc gcacggagag gggtacgggc atgagcgcga
     1201 caacgattga ctacggcact ctcggcgagc gtgccgcggc cggtgtgaag ccgagggcgc
     1261 tgtccgacga gcagaaaatc gcccgcgcga aggaggcctt cctcgccggc atggacgccg
     1321 acatggcgac ctacctcgag tcctgcatcc attgcgggat gtgcgcggag gcctgccact
     1381 tctacctggc caccgaggat gcgaagtaca cgccgatcta caagctggag ccgctcaaga
     1441 aggtctaccg tcgcgagctc gcgccgaccc gctggctgaa ccggctgacc acgcgcgaca
     1501 tcaccgtggc cgacctcgag cagtggcaag agctggtgta cgactcctgc acgcagtgcg
     1561 ggcgctgcag cctgctctgc ccgatgggca tccacatcca gagcatgatc caggtggtgc
     1621 gcaacggcct ggcggccgcc gggctggccc ctgcggagct gcaggcggtc tccgacgagc
     1681 agaaggacaa ggcgagcatt ctcggcgccg ggcccgacaa gctgcgcgag atcgtggagc
     1741 gcgtccgtgc caagggcatc gacgtgccgc tggacaagga caaggctgag gtgctggtgc
     1801 tgtcctccgc gctcgatttc ctcaaggacg agagcgtgct ggcggcgacg gcgaagatca
     1861 tgaacaagct gggcgccagc tggaccatcc gcagcgatgg cttcgagtcc gccaacttcg
     1921 gtgcgctggc cggcgacaag caggcccaga agcgcatcag caagcgcgtc atcgacgcgg
     1981 ctatcgccgc cggcgccaag aaggttatcg tcgccgagtg cggccatgcc tacccggtcc
     2041 tgcgctggga ggcggccacg ctgtacggca aggagctgcc cttcgagttc ctgtccgtag
     2101 ccgagtacct cggccaggag ctcaaggccg gacggctgaa gctgaagaag gccggcaacg
     2161 gcgccgccca cgtcaccttc catgacccgt gcaagatcgg gcgcatgggc gggtccttcg
     2221 acgagtcccg cgacgtgctc aaggctctcg gcgtcaagct taccgagatg gaatcgcacg
     2281 gccgcaccaa cctgtgctgc ggcggcggcg gcggcgtggc gatgctgaag agtgcacaga
     2341 cgctgcgccg caaggccttc gagctgaagc gcgaggaggt cgaccggacc ggcgcaccga
     2401 cggtggtgac tgcctgcaac acctgcatgc agaatcttga gatgggcaag gcctggctga
     2461 actgggacaa gagcgtcgtg aacctcgtcc agctcgtcag cgacaacctt gcctgagccc
     2521 ggcacgccgg cagcgaacga cttacaagga tcggggtatt aaattatgag caatcgagtc
     2581 gttgtcgacc cgatcacccg catcgaaggc cacctccgcg tcgaggtgca gatgcagggc
     2641 gatcgcatcc aacaggcgta ctcttcgggc acgatggtgc gcggcatcga gatcatcatg
     2701 cgcggccgcg acccgcgcga tgcctgggcc ttcacccagc gcatctgcgg tgtctgcacg
     2761 ctggtgcacg gcatggcatc catccgggct gtcgaggatg ccctcaagta cgaaatcccg
     2821 cacaacgcgc agctgatccg aaacctgatg atcggctcgc agtacgtgct ggaccacgtg
     2881 atgcacttct accacctgca cgcgctggac tgggtcgacg tcgtctcggc gttgagcgcc
     2941 gacgcggcgg cgactgctga tcttgccaag gcggtttcgc cgcagtggcc gaagtccacc
     3001 gagggctatt tccgcgacgt caagaagaag ctgcagggct tcgtcgaagg cgggcagctg
     3061 ggcattttcg ccaacgccta ctggggtcac ccggcctaca agctgccgcc cgaggccaac
     3121 ctgatggcgg tggcgcatta cctcgaggca ttggcctggc agcgcgacgt ggccaagctg
     3181 cacgcgatct tcggcggcaa gaacccgcat ccgaacttcg tcgtcggcgg cgtgcccagc
     3241 cccatcgacc tgaactcgga ctcggccatc aacgccgagc ggctgcaggt ggtgcagaag
     3301 atcatcgaca tgatgagggt cttcgtggac caggtgtacg tccccgacac gttggccatt
     3361 gccggcttct acaaggactg gttcggtcgc ggcgaggggc tcggcaactt catctccttc
     3421 ggcgacctgc cgtcgcggag catgaccgac tcgtcgagct tcctgattcc gcaaggcgtc
     3481 atcctcaatc gcaacctcaa cgacatccag gaagtggact tcgcggaccc caacgagatc
     3541 caggaattcg catcgcactc ctggtacaac tactccggcg gcaagaacca gggactgcat
     3601 ccctgggacg gcgagacgga cctcaactac gagggccgtg gcgggccggc ggcgccttac
     3661 aagcagctca acgtcgatga cggctacagc tggctgaagt cgccgcgctg gaagggccat
     3721 tcgatggaag tcggtccgct ggcccgggtg ctcatcatgt acgcgaaggg ccacgaccag
     3781 acgcgcgagc tggtgaacta tgccctgagc accctggacg ccccggtgga ggccttgttt
     3841 tcgacgctcg ggcgcaccgc tgcccgcacg ctcgagacca agatcatcgc ggacgccatg
     3901 cagggctgga tggaccagtt gctcggcaac atccgcgccg gcgacaccag gaccttcaac
     3961 gagatccagt ggaatccctc cagctggccg aaggtcgcca agggcgtcgg gatccacgag
     4021 gcaccgcgcg gcggcctgtc gcactggatc gtcatcaagg acggccggat cgacaactac
     4081 cagtgcgtag tgccgagcac ctggaacgcg ggtccccgcg atccgcaggg tgtaccgggg
     4141 gcctacgagg cggcgttgca ggacaatcac cagctgtacg atcccgagca gccgatcgaa
     4201 atcctgcgca ccattcacag cttcgacccc tgcctggcct gcgccgtgca cgtcacggat
     4261 ccggacggca aggagctgat caaggtgaag gccctgtaag cgagcccggc ccccccggcc
     4321 gattgtggcg aaaaacccag gaagcccggc gaaagccggg cttcctgctt tgcgggggat
     4381 ggtatgctgc ccgggttccg cgcgggtcgc gggaggggcc gccgatggac gagtttttta
     4441 ccccccagca agtgatcgat gttgcgaatt ctgcctgggc atgggcacgc gcccaggtct
     4501 tcgcgctgcc caacctgttg cagctgctca cggtagcggt cgggctggct gcggccgcat
     4561 tgctgcgacc gcgcttgcag cggctgatcg agcgcctcgc ctggggcgca cgctggaagc
     4621 gggccgtgga tatgctgcgg tcgctgctct tgcccttgct ctggctggtg ctggtcggca
     4681 tcgcatggca ggcggccgtc attcaggcct ggccggcacg catcctggaa atcgctgcga
     4741 gcttgctgat ggcctgggtc gtgatcaaca tcgcctcgag cctgattcgc aacccagtgt
     4801 gggcgcgcgc agtggcgatc accgcgtgga ccatcgcggc gctcaacatc ctgcggctgc
     4861 tcgatccgac aatcgcgctg ctcgattcct tcgccatcca gttcggcgcg gtgcgcatat
     4921 cgctgctcac ggtaacgaag tcggtgttcg ccctggcggt gctgctgtgg ctggcgacgg
     4981 tggtgtccga gaccctggaa tcgaggcttc gccacagtcc caacctgacg ccgtcagtcc
     5041 aggtcctgtt tggcaagctg ttgaaattca gcctcgtcac catcgcggtg ctcgccgcca
     5101 ttgccaccac gggcatggac atgacggcgc tcgcggtgtt cggcggcgcc atcggcgtcg
     5161 gcatcggcct cggcctgcag cgcgtcatcg ccaacctgtt ctccggggtc atcctgctgc
     5221 tcgacaaatc catcaagccg ggcgacgtcg tcgcggtggc gggcacctac ggctgggtga
     5281 acacgctcgg cgggcggtat gtctcggtcg tctcgcgaga cggcatcgag cacctcattc
     5341 cgaacgagga attcatcacc acgcgagtgg aaaactggac gcacaccgac agcaagatcc
     5401 gggtcaagat tcccatcggt gtgcactacc agtcggatgt cgagcatgcg ctggcgatct
     5461 gcgtgcaatg cgcagagcag gttccccgcg tgctggcgga ccccgcgccc gtgtgccagc
     5521 tcaagtcctt cggcgacaac tcggtggatc tcgagctgcg gatctgggtc ggggatccga
     5581 tgaacggagt caacaacgtg cgcagtgaaa tcctgcgcct ggtctggcgc gcctttcacg
     5641 agcacggcat cgagattccc tacccgcaac gtgatctgca cgtgcgcgcc ggcgagcccc
     5701 tgcaggtagt cgtggccgcg gcagccgcgg agcgctcgac atgagcgagc ggcaggggct
     5761 gggacccgga gctttcgagc cgccccggcc gggcacgagc tacgagccct atgtgccggc
     5821 acacgccagc ttgcaggagt tcaccttcaa ggccatcggc ctgggcatag ccttcggcat
     5881 catcttcggt gccgcgaatg cctacctcgg cctgcgcgcg ggcctcacca tcagcacctc
     5941 catcccggtc gcggtgatga ccgtggccgt gttccgggcg ctggcagcag tcgggcgcag
     6001 cggtaccctc ctcgaggcca atctttcgca gacggtcggc tcggcctcca gttcgctggc
     6061 cagcggcatc atatttacct tgccggcgct gttcctgtgg ggactgtcgc cgagcctgtt
     6121 gcagatgacc atgctggcca tgtgcggcgg cgtgctcggc gtcctgttca tgatcccgct
     6181 gcgacgcttc ctcattgagc gggagcacgg caagctgccg tatcccgagg gcaccgcctg
     6241 cgcggaggtg ctggtcgcca acgaggtcgg cggcggcaat gcgcgcttcg tcttcatcgg
     6301 cctcgcggcg ggtgcggcct tcaaggcgct cacctcctgg gcgcgcgcca tccccgatgc
     6361 cgtgcacctc aagctgccgt ggccgcgcaa ggccgagctg ggcatggacc tgtcgccggc
     6421 gctgttcgga gtcggcttca tcctcgggcc gcgcattgcc gcggtgatgg tcggcggcgg
     6481 gctgctgtcc tggctggtgg tgatcccgtt gctggactgg tggggcgcct cccgcagcct
     6541 gccgctctat cccgagaccg tgctgctgat gaccgacatg agcccgagcc agctgtggtc
     6601 gcgctacgtc cgctacatcg gcgcgggcgc ggtcgcgacg gccggcatca tcaccctcgt
     6661 acgcagcctg ccggtgatgg tggacagctt ccgcatcggt ttcggccagc tgcagaagca
     6721 gcgcgccgag cacccggtgg cggtgccgcg caccggtcag gacctgccgc tcaaggtggt
     6781 cggcatcggc gcggtgctgg tcatcgccac actcggcctg gtaccggtcc cattcggcga
     6841 ggtcgggggc gtcgtgcatc gcctcgtggc cgccgtctgc gtggcggtgt tcgcgttctt
     6901 cttcgtgacg gtcgcctcgc gcatcgtcgg cctcgtcggc gtcaccagca atcccaccag
     6961 cggcatgacc attgccgcgc tgctcggcac gtccggcatc ttcctggcca tgggctggac
     7021 cgacatgacg ggcaaggccg cggcgatcac cgtcggctgt gtcgtagcca tcgccgcctc
     7081 gatcgcgggc gacacctcgc aggacctgaa gaccgggtac ctgctggggg cgacgccgcg
     7141 ccggcagcag gtcggtgaac tggtcggtgt gctgaccgcg gcggtgttcg tgtgcctggc
     7201 ggtgctgttg ctggccgaga ctttcggctt cggcagcact gagttgccgg cgccacaggc
     7261 cacgctgatg aaactggtca tcgacggcgt gctggaccag tccttgccgt ggggcctggt
     7321 ggcgctgggc atcggtatcg ccgttgcgtg cgagctgctg aagattccgt ccctgccttt
     7381 tgccgtcggc gtgtacctgc cggtatcgac catgacgccg attttcctcg gcggcttgct
     7441 gcgattgtgg gccgagcgca gcgcgaccgg caccgatgag ctgaacgagc ggcgcgagcg
     7501 cggcatcctg ctcggcagcg gcttcgtcgg cggcgagggc ctgctgggtg tcggtatcgc
     7561 cggggcggcg ttcgtgctgg gccggcggcc gctcggcatc ggcacgggct ggctgggcgg
     7621 cgacgctgtg gccgcgctgg tcggcctggt ggcgttcacg gcgttcgcgg catggtttta
     7681 cctgcaggtg cggcgtcgtg cggactgagg acgcaggcaa ggggcgcctc ggacgctgga
     7741 cgacgctggc ggtggccgtg ctggtcgtcc tggcgggcct cgggccggcc gccgcggcgc
     7801 cggacccggt cgaggtggcg gagacgatcc gccgctccgt ggagcagctg cgcagcaccg
     7861 gtgaagtcac ggtggcgggc cgcaagttgc gctcggcacg cactctcccg gaagtctacg
     7921 aggcgcgcgg cttcacgctg ttgtggcgtg accgagtgag cgaggaggcc ctgctcggcg
     7981 agatcgcggc gatcagcggc gaaggcctgg atccggggga ctatcatttc gaggccttgc
     8041 gcgccgccct cgatcggcgg cgccagcagg ccgacagtgc ggcggcggcg gccaccgccg
     8101 acctgctgct caccgatgcg ttgctgcgcg tcgctgcgca cctccaccag ggcaaagtgg
     8161 atcccgccac cggcagcccg cgctgggacc tgcttgggcc ggtgcgcggc gagtccggcg
     8221 cggcgctggc cacccgcatt gcctccagcg gggcggtcgg attgcagctc gccgagctac
     8281 ggccggtgca gccgatgtac ggccggctgc gctcggcgct ggcccgctac cgcgtgatcg
     8341 agcagcaggg cggatgggag ccgttggcgt ccgggcgcgt gctgcaggcc ggcctggagg
     8401 atccccgtgt gccggccttg cggcgtcggc tggcggccac cggcgatttc accgggatcg
     8461 tggtggactc accgcgcttc gacggcgcac tcgaagaggc ggtgcgacgt ttccagtcgc
     8521 gtcacgggct ggagccggac ggcattttcg gtccggcctc gttacgggtg ctgaacgtcc
     8581 cgttgaaggt ccggcaggac cagctcaggg ccaatctgga gcgcgcgcgc tggttgctgg
     8641 tggacgtccg aggccgcttc ctgctggcgg accccgcggg gcgccgcgtg gtgttgatgg
     8701 acaacagcga gcccgtgttc cgggtcgcgg cggaattttc gacggccgca cgcgcggtgc
     8761 cgccgttccg cgcggagctg cgctacctgg tggcgaatcc cgactgggtc ctcactccgg
     8821 gcctcgtcga gacgcaggtc gcgccactcg cccgacgcgc gccggagcag ctcagcgcgc
     8881 gcggcctgca ggtattcaat gccgcgggcg acgcactcga tgcggcggcg gtggactggt
     8941 ctgcgccggg cggagtcatc gtccggcagc tgcccggcga gcgcagcttc ctcggcaacc
     9001 tgagatttcc cattcacaac cacgccggcg tcttcctgca cggcggcccg gcacgcggcg
     9061 aggcgctggc aggttcggtg cgcctcgcgg acgccgccgg cctggcgcat gccctggcgg
     9121 ccgggcccac cgcctcgagc gccgaaagcc tggccgcggc cctcgcggcg ggtactccgc
     9181 gcaccttgtc gctcgctacg ccggtgccgg tgctgtacgg gtcctggacc gcctgggtac
     9241 aggccgacgg cacggtgtcg ttccgggccg ggtacgagga ccgggacgct gcgatcatcg
     9301 cggcgcttgg gcgaggtgcg gatcgccggt agcaccgtga tcgcagcctg gcactgcccg
     9361 ccgcgtcgcc atggtttgcg ccgctgcccg caccgcgcat aattgcggtc ccatgcaagg
     9421 gaaacagcac atggagcagc tctccggcct cgacgccgcg ttcgtctatc tcgactcgcg
     9481 caatgccccg atgcacgtcg gcgggctgca cgtctaccag cctgagtccg gccggttcga
     9541 cttcgaggac ttccaggccc acatcgcggc gtgcatcgag ggttcgccgt tgttccgccg
     9601 ccggctcgtg caggtgccgc tgcacctcgg acggccgttc tgggctgagg atccgaattt
     9661 cgagctggac ctccacctgc aacactcggc gctgccgcgg ccggccgact gggcgcgttt
     9721 gtgcgaactg gcggcgcgca tgttctcgcg tccgctggat ccggaacggc cgctgtggca
     9781 cctcactttc gtgtcgggcc tggacgcctg cggtgtgttt ccgcccggca gcttcgcggt
     9841 gatctcgcgc gtgcaccacg cggcagccga cggactcggc acgctcgaca tgttcaatgc
     9901 cgtctggagc gccgaaccgc ggcggcggaa acgcaagacc gtcggcacgc ggcgagcgcc
     9961 tgtggagccg gtgccgggcg ccctgggctt gctgaagcgc agcttcggcg gcgtgttccg
    10021 gcaaccgggc gagaccttga gctgggcggc cagcctgctg cgcggcggcc tcggcgccgg
    10081 gcgcgagttc atcgcgcggg atcaccatgc gccgcccatg ctgttccagg cgccgcgcag
    10141 cccattcaac gtaccgatca gttcgcggcg gtgcttcgat gcggtcaagc tgccgctcga
    10201 cgaaatccgg aacatccgcg cggccgtgcc gggcgccacg gtcaacgacg tggtgctggc
    10261 ggtctgcagt ggcggcctgc gacgctacct gcagagctac gcgagcctgc ccgagaagga
    10321 cctggtcgca atggcgccga tttcgatccg cgggaagatg gaaaaaggct ccaaccgcgt
    10381 gtcggccatg ctgatcccgc tcgaggtggg ggcgcgcgac ccggtggagc ggctgcggcg
    10441 catccacgcg cacgcggtgg acagcaaggc ctacacggac gcgatcggcg cggcgacgct
    10501 cgcggacacc tcccgtttcg tcccgtactc gctcgctact gcggccgccg gcctgtactc
    10561 gggactgcac ctcgcgcgct atcaccggcc acccttcaac ctggtgatca ccaacgtgcc
    10621 cggcccgcgc gagcccctgt acctcggcac cgccaggctg ctggaagggt tcggcaccgc
    10681 gccggtgctc gacgggctcg gcctgatcct ggtggtcacc agctacatgg acaccctgtc
    10741 ggtcagcgcg acggccgcgc acgaggccgt gccggacgtc gagcgcctga tcgaatcgat
    10801 ccgcgcgagc ttcatggagc tccgtgacgc ggttgctgcg cgcgccaccc ccaaggcggc
    10861 ccgcggccgg gcgagtcgtc cgcgagtcaa gcgagggcag ggtgcaggct gaagaggagc
    10921 tggggccgcc gccgctgatc tggcagctgc tggagggccg tgcagtcatc gagctggggg
    10981 cgatggcctg gctctatccg ctgttgcgcc aggcgccggc gggggacggc cacccggtac
    11041 ttgtgctgcc cggcttcctg gccagcggca gctccacctt tcccctgcga cagttcctga
    11101 aatcgctggg ctacgcggcg catcgctgga agctcggccg caacctcggc ggggtgggtg
    11161 agaccgagga gcgcctgctg gcgcgcctgc gggagctcag gcgccgttac ggccgcaagg
    11221 tcagtgtgat cggctggagc ctcggcggca tgtatgcgcg tgaactgtcg tggctcgcgc
    11281 cggacgacgt ccggctggtg atcacgctcg gcacgccgtt caggcaccac acgggcacca
    11341 ccgtgacgct gttgtacgag gacgtcagcg gccagcggga agccaccatg gatccgcgcc
    11401 tgctcgcgcg cctggcccag ccgccgccgg tgccggcgac gtcgatttac acccggaccg
    11461 acggcgtggt gcactggcgt tgctcgctcg agcatccttc gccgacgacg gagaacgtcc
    11521 gcgtgcacgg cagccactgc ggcctgggcc acaaccccat ggcactctgg gcgatcgccg
    11581 accggctggc gcagcccgag ggcgactggc agcctttcgc gcggcatgcc ttccggcgtt
    11641 tcgcctaccc ggcgccggat cgcggcgact gaccatgcag gagttcgtcg acgctgccaa
    11701 ccagctcatg tgggggtcgc tgctgatctg gctgctgctc ggtgcgggat tgttgttcac
    11761 cgttttgtcc ggcttcatcc aggtgcggca tttcgggcac accttcggcg tgatgaagcg
    11821 cagcttccgc cgcgagcacg gcggcatatc gtccttcgag gcctattgca ccagcctggg
    11881 cgcgcgcgtc ggcaccggca acctggccgg cgtggcggta gcgctggaac tcggcgggcc
    11941 gggtgcggtg ttctggatgt gggtcaccgc catgctcggc atggcgacga gcttcttcga
    12001 gtccaccctt gcgcagctgt tcaaggtgcc gaacggtgac ggcagctacc gtggcgggcc
    12061 ggccttctac atgcagtacg gactgggcaa gcgctggatg ggcatcctgt tctccgtgtt
    12121 cctgatcgtg tcctacccga tcgccttcaa cggcgtgcag tcgaactcga tgtctgcggc
    12181 ggtggaacag gtgaccggga tcgacgccgg ctggaccggc ctgctgaccg ccttcctggt
    12241 cgggctggtg atcttcggcg gcattcgccg catcgcgcgc gtcgcggaga tcgtcgtgcc
    12301 cttcatggcc atcgcctatc tgggcgttgc gctgtggctg gtgctcagtc atctggaccg
    12361 cctgcccggg gtggtccggc tggtgttcga gagcgccttc ggcattggcc aggcggccgg
    12421 cggtgtgctc ggttacggca ttgcgcaggc actcatcaac ggtgtgcggc gcggcctgtt
    12481 ctccaacgag gcgggttccg gcagcgcgcc catcgcggcc gcgaccgccg acgtgcgcca
    12541 cccggccagc cagggctacg tgcagatgct cggcgtattc accgacacca tcgtaatctg
    12601 ctcctgtacc gccgcgatca tcctggtgtc gggccagttc gaggccggcg ccggcgtgac
    12661 cggcatggcg ctcacgcagg gcgcgatgag tgcgctggtc ggcgactggg gcgcgggctt
    12721 catggcggtc gcactgctgt tcttcgcttt cacctccatc atggcgaaca cctactacgg
    12781 cgaaaccagc ctggcgttca tccgttccga ccagcgactg ctgaccgcct accgttgcgt
    12841 cgtgctggcc gggattttcg ccggctcgca aatcgatctc ccgctggtct gggcgatggc
    12901 ggacctctcc atggccttca tgaccatcgt caatctcgtc gccgtgctgc tgctctccgg
    12961 gctggtcatc aaggtgatgc gcgattacaa cagccagctc gcactgcacg tcgagccggt
    13021 gttcgacctc gcccgctttc ccgacatcgc acgccgcctg ccgcgcggcg gctggtgaac
    13081 gcagggggcg gctcgacgcc atgaaccgct tcgtgcgcgc catgcagtct ccggaggcac
    13141 agaagctgct tctcaactgc tatccgccgt acctgatgac cgggatcacg gtgcaccgca
    13201 ttacacccga ctggcggcgg gtcgaggtga gcatggcgct gcgctggtac aaccgcaatt
    13261 atttcggcac gcacttcggc gccagcctgt acggcatgac ggatcccttc tacgcgataa
    13321 tgctgtcacg caatatcgga cccgggtatt tcgtctggga tcgctcggcc acgatcgatt
    13381 tcctgcgccc gggtcgcggg cgggtcagcg cgcgattcga gctgacggca gagatgattg
    13441 agtcggtgct ggcggccacc gcgtcgggcg agaaacacca gccgacctgg caggtggagg
    13501 tgcgcgacgc ggcgggtgag ctggtcgcgc aggtcagcaa gacgctctac gtgcgccgca
    13561 agccgcagcg ctgaatcggc ccgcgccgcc ggcgcgatat agaatcaggc cctacttccg
    13621 cgcatggacg gcgctccgat gcacgctcgc aagctgtcac tgcttctgtt tgccgcgctc
    13681 ggtgtcgccg cggcgactgg catcgtgcgc tgcatcgctc acggccctga gcgcgtcacc
    13741 acggcagcgc tgcagtctgc cctggccggg atcgaccggc tggcggccga gtttccctcg
    13801 cccggtgatg cgccgctggc gtttcccgcc gaccatgcgc cgaagcccgg gcagttcgtc
    13861 gagtcgtggc tgttcgccgg gctggtgcga gacgatgcgg gggaggcctg gggctttcag
    13921 ctggcattcg aacgtgtcgc cgtgcaggcg gaggcggtat cacgggaatc agagtgggcc
    13981 gtgcgcgacg tgtggcgcgc gcgacttgcc gtcgaacggg cagggacgcg cgcggcggcc
    14041 ggcgagcgga tcagccgggc ggcgctcggc ctggcgggcg caagtgacac accggcggcc
    14101 gcatgggtcg aggactggcg gttcgagctg caagagtcag gcgaccggtt tcgcctgcag
    14161 ggcgccgccg gcggcgccgc ggtggacctg cacctgcgcc tgccggcgac cgctccggtc
    14221 gcgatcggac gtgagcccta ccgcggctac tggtggcccg ggctagaggt ggccggcacg
    14281 ctcgtgctgg aggggcgcga gtcggcagtc acgggccggg cgatgctgga tcgtgtctgg
    14341 ggccggggcc tgccggtcgg ccgcggccag ctcgcgctgg cgggactgtg gctggagctc
    14401 gcggacggcg cggcgtaccg ctgcgaacag ttgcgccggc gggccggagg cggcaccccg
    14461 ctgaccgagt gcaacacggt tccgccggcg gacgcgctcc tgctggcgcc gcgcgacgac
    14521 ggctggcgtg tcctcgacgg cgtccggtac cccttgcagt ggcaggtaca gctcggcgcg
    14581 gaagtcggca cgctcggctt gcggccgctg tccgcgagcg gcgcggtatg gttggacggc
    14641 gcctgggtcg gcatcgtggt cgcggaaggc gaccgcgagg gctggggcct gctgcaactg
    14701 agcaatttcg cgccgccttg agcgcgcggc atcggcaccg gagtgatcga gtcatgaaga
    14761 tccacaacat catcacgggc ctcgccctgg caggcgggtt gcttgccggc tgcgggcgag
    14821 gcgcagaggt tcccctcggg tcgggcgagc ccggcccgct gcccgccgcg cagcagcgca
    14881 gcggcgatcc ggcactcggc tacgaaatcc tgctcaatcg gggctacgtc agctgcggcg
    14941 tgccgctcga cgcttacctg gcgtcggcgc cggatcgcag tccggagcct gcgttgcacg
    15001 ggcgagtcgg ccgtaacgcc gagctaccct acgatctgaa cgcgaccatc gacaccaacg
    15061 gcgtcgagct ggtcgtcagc aactgcctgg cctgccacgg cgggcgcttc aacggcgagc
    15121 tggtgatcgg actcggcaac gccttcgcgg acttcagcag cgatccgatg cccggcatcg
    15181 agggcatcgg gctgctggtc gaggatggcg caccggcaca ggcctggcag cgctgggccg
    15241 atcgcgtcgc cgcgatcgcg ccttacatgg tgaccgacac cgtcggggtc aactccgcgc
    15301 ccaacctgac catggcgctg atggcgcacc gcgacccacg gaccctgcgc tggcacgacg
    15361 caccacggct cgagccgccg ccccgcgagc cgctgccgac cgcggtgccg ccgtggtggc
    15421 gcatgcagaa gaagcacgcc atgttctacc acggcggcgg acaaggcgat cacgtgccct
    15481 acatgatgct gaaatcgctg gtctgcaccg acgatctcga agaggcacgc atgatcgacg
    15541 gctggttcac gcacgtggcc gcctacatca agagcttgga gccgccgcgc tggccgtggc
    15601 cagtggacga gtcgctggcg ggcgaggggc agaaggtgtt cgaagcgcac tgtgcggcct
    15661 gccacggcac ttacggcgag cgctggacct atccgaatct gctagtgccg ctggagcagg
    15721 tcggcaccga tccggcctac gcgctccagg cggtcgaggc ggaacgcttc atccgctggt
    15781 tcaacggatc cttctacggc cgcggcgcac aggcgcggcc ggcgcgcggc tacgttgccc
    15841 cgccactcga tgcggtgtgg gcgaccgcgc cttacctgca caacgactcg gtgccgagca
    15901 tcgcggcgct gctggacagc agccgccggc cgacctactg gcggcaccgt accgagccac
    15961 gggaatacga ccaggccgcg ctgggctggc gctacgaggc gttgcaacac ggcaaggagg
    16021 gcgccgggga cgccgacggg gcgcgcagga tctacgacac cacgcaacgc gggtacggca
    16081 acgggggcca cctgttcggc gactcgctcg acgagggcca gcgccgggcg ctgatcgagt
    16141 acctcaagac actctgaccg gctccggttc agcgccccag cagccggagg ttgcgcgtgc
    16201 gctgccgggg ccgctcgacc tcgaagtgca cggccaccat ccagtcggcg tagcccgggt
    16261 ccagcttcct gagggtgtgc atcaattcct gccggaaact ctggccgacc ggttcgacgc
    16321 ggtccggcca gatgcggaac ttgaggcgca ggaagctgcg ccccgcgctg gtcgtctgca
    16381 gcccctcgac cgaaggcggg ctgcgcatga tgcccgggaa ttgttccgcg accgtcggca
    16441 gcaggcgctc ggccgccgcg atcatccgtg cgcggacgtc ctcgtcgtcc gacagcgata
    16501 cgtcgacgat cagccgcacg tagccgcgcg gatacgagac cacgttggac accgtgcggt
    16561 tggggatgtt gaccagcgcg ccgtgcgagt tttccagcac gatgaaacgc atgccgatgg
    16621 cgcgcacgat gccggtctgc ccgccgatat tgaccatttc tcccatgtcg atgagattcg
    16681 agaaaatcag ggtcaggccc gtgaccacgt cctgcacgac gccctgcgag ccgaagccga
    16741 ccgccaggcc gatcaccgag gcgctcgcca ggtacgccgt catgctgatg ccgaattcct
    16801 gcaggatgaa cccgatggcg atgaagtaca tcgtgaatac ggcgacactc gtggccagcg
    16861 agatggtggt gcggagcttg cgcaccgagg ccgtcgcctc gctgcgcgtc agccgctcgc
    16921 cgccgacacg taccaggtgc accccgaaat gcagcgcgac cgctacgccg agaatcaaca
    16981 gcgtgcggcc cagcaatggc atcgcggtga aaatctccgt cattccggcc tcgctggatg
    17041 gcttgtcgtc accagtttaa ccgcgcaggg tcgctgcgcg ccgccgcagc acgaacttct
    17101 cgagttccac cagcggcact accactgcgg cgaccagcac cacgagcagc cagtcgccca
    17161 aatccagcgg tgccgtgccg aacagtgcgt tcatggccgg caggtaggtg aacagcagct
    17221 gcaagccgaa gaccgccatg acggcgccga tggcgatgcg gctgccgaac agaccgttca
    17281 acgtgagagt gggcgccagg atgtggcgca cgttcaacag gtagaaggcc tcgaacagca
    17341 ccagcgcatt gaccgccagc gtgcgcgcgg tctcgagttc atagcctgcc gcgcgtgcct
    17401 cgacgaacag gccgaacgtc cccgccacga cgatcatggt gacgaagctg atgcgccaga
    17461 tgacgaagcg ccccagcagc ggctcgtccg ggtggcgcgg cgggcgctgc atgatgtcct
    17521 gttcgggcgg ttcgaatgcc agcgccagcg ccagggtcac tgcggtcacc atgttcaccc
    17581 acaggatctg cagtggcgtg atggggagcg agcgcccaag aacgatcgcc agcaggatca
    17641 tcatcgcttc cgccgcattg gtgggcagaa tgaacaggat ggccttgcga atgttgtcat
    17701 acaccgtgcg gccttcctcg acggcgcact cgatggaagc gaagttgtcg tccgacaaca
    17761 ccatttctgc agcctgcctg gcgacctcgg ttccgccgcg gcccatcgca acgccgacgt
    17821 ccgcgcgctt gagcgcaggc gcgtcgttca cgccgtcgcc ggtcatggcg acgacgtggc
    17881 catgggcctg cagggcctcc acgatgcgca gcttgtgctc cgggctcgag cgggcaaaga
    17941 cgtcggtcct ctgcaccact tcggcaagcc gtgcatcgtc gagtctctgc agctcttcgc
    18001 ccaggacggc gccctgaccg tcgccgatgc ccattgcctg tccaactgcg cgggcggtgc
    18061 tcagatggtc gccggtgatc atcttcaccc ggatacccgc gctgcgacat tcggcaaccg
    18121 ccttgatggc ctcgtcgcgg ggcgggtcga tcagtccgac caggccgagc aggctgaggg
    18181 agtcctcgag ctcgctggtc ttgagctcgc cgcgacgtcc attgggcggc gtgcgcctgg
    18241 caagggccag cacgcgcagg ccgtcgaccg ccagtcgttc cgcgctcgcc agccaacgct
    18301 cccggtccag cggcatggcg ccatggtcac tcagcgcgtc gacacacatc gacagaattc
    18361 gctcgggtgc gcccttgacg tagatgaacc gcgcgccgtc atggtcatga tgcagcgtgg
    18421 ccatgaagcg gcgttccgac tcgaacggga tggcgtcgtc ccgcggcgcg ctcggccgga
    18481 gtctgtcggg ctccagcccg gccttcatcg ccagcaccag cagtgcgccc tcggtgggat
    18541 cgccacgcag cctgctgccc tgggctcctt gctcaaactc cgcatcgttg cagagcaggg
    18601 ctgcgcgaca cagttccatc agcgccttgt cggtggacgg atcggcctcc tcggcccccg
    18661 actggattac gccgagtggg tcatagccca cgccggtgac ttggtactcg cgggactgca
    18721 atacgacccg gctggctgtc atttcgttgc gcgtgagggt gccggtcttg tcgctgcaga
    18781 tcaccgacac cgagccaaga gtctcgacgg ccggcagacg gcgaatgatg gcgttgcgcg
    18841 ccgccatgcg ctggacgccg atcgccaagg tgatggtgat gatcgcgggt agtccctccg
    18901 gtatggccgc gaccgcgagc ccgaccgcgg ccatgaacat gtcctcgagc gcgtagtcgt
    18961 gcagcagcac accgacgacc atggtcgcag cggcgacgcc gccgatcacc aggctgagca
    19021 gcctgccgaa gcgctcgagt tggcgtgtca gcggcgtggc cagcgtctgt acgcgcaaga
    19081 gcagcgcgct gatccgcccg atttccgtag cgccgccagt ggccgccacc acaccggtgc
    19141 ccacgccggc cgtcaccagg gtgccggacc aggccatcga cgcacgatcc ccgagcgcgg
    19201 cgtcacgcgc caccggggcg gtggattttt ccaccggcac cgactcgccc gtgaggattg
    19261 cctcgtcgat ctgcaggttg cgtgtcttca acaggcgcaa atccgccggc accaggtcac
    19321 ccgcctgcag caccacgaca tcccccggca cgagctgttc ggcggcgatc tcgtgcttgt
    19381 ggccttcgcg gatgaccatg gcgcgcggcg acagcatggc gctgatggct tccagcgcgc
    19441 gctcggcctt gccctcctgg atgaatccga tcagcgaatt gatgacgacc acgcccagaa
    19501 tgacgccggt gtcggcccat tcgcccagca gcgcggtgat ggcggcggcg accagcagca
    19561 cgtagatcag cacgttgttg aactgggcgg cgaggcggcg cagcggaccg cgtcgtggcg
    19621 gcggtggcag gagattcggg ccgtgcgcgg cgagccgctc ggccgccgtg gatttgttca
    19681 gcccctcggc gccactgtag agtcgctcga gagcctgccc ggcatccagc gcgtgccagg
    19741 cctgggagcg gacggtggtg tgcggtgctt ccatggacct cacccgcgcg ggaacgagac
    19801 ggcggggacc tggcgggatg tggagacgag tttcaggtta gcgccgaacg acttgattgg
    19861 gaaggatttc gcgactgttg gcggcgtctt gcaaagagaa ataagttgcg gtgcgacatc
    19921 ttaaatgata tataatcaat gcatgaatat cggacggccc accgtgcccg tcaaaacccc
    19981 gcgaagccgg gattctcttg ccgtcgccgg cctcgcattg ctgctcgccg tcctgcagcc
    20041 gagtcccgcg gcggcgcagg aaaacggcga gccggccgcc gagttggaac gcgtcgttgt
    20101 ggtcagcact cgcagcccgc ggccggtcag cgaagtcgtg ggcatggtga cgatcctcga
    20161 cgacagcgac gtcgcagcga tcatggccac caacgaggac gggctgtggc gctacacgcc
    20221 gggtatccag gtggaatcgt ccgggtcccg ctatcccgcg cacagcctca gcatccgcgg
    20281 catcggcggc aaccgcgtgc tgatggaact cgacggcatc ccgatccagg agcgtttcct
    20341 ggtcggcaac tttgcggacg ccggccgctc cagctcggaa ttggatttca tcaggcgggt
    20401 cgaggtgctg cgcggtccgg cctccagcct ctatggctcc agggccatcg gtggggtagt
    20461 ggccgtgtcc accttcgacc cggacgacct ggcgctgggc ccgggcgcgc cgggcgccct
    20521 ggtgcgcggc agcttcgcaa gtgactggga cgccttcggg gcaagcgcga tccgcggctg
    20581 gcagcacgag tctgtgggcc tgttgctggg tgccagtcat cgccacggca acgagcccga
    20641 tcgttccgcg aatcccgatt cgccggatcg gctcgaccgt gaccggcagg ccatgctggc
    20701 caagttgacc ttgcgggacg acgaggacaa cgggctgcgg ctgacgctgg atggcgatcg
    20761 cgtcgacacg gcggcagaga tcaacagctt catcggccag gggcgcttcg ccaacaccac
    20821 gggcctgttc ggcgatgacc gctcgactcg cacgggtatc gcgctcgacg ggcacatggc
    20881 gcaccgggaa tacctggtgg aggggatcgt gtttcatcgt gaaacgcaga cccgccagga
    20941 caccgtggac ctgcgtgagg cccttgcgcg accggtgaga atcgaccgcg gtttccgcta
    21001 cgacaccacc atcagcggcg tacgcaccag agtgtcgagg cagttcgaac atggcgggct
    21061 gcgtcaccgc gtgatgctcg gcgccgaatt ctcgcgcagc gagctggacg agatgcggga
    21121 cgccgcgttg accggcatcc tcgacggcca gacgaccaac gtagtgctgg gcgagtcctt
    21181 cccgctgcgt gacttcccgg tcaccgagag tcgcgagacc ggcgtattcc tgcaggacga
    21241 gatcgactgg ctgtccggac gctggaccgc catcccgtca ctgcgtttcg atcgcacccg
    21301 catccggatt cgcgaagacg acgcgtggcg tcgggcgaac ccgcaggccg acgccgccga
    21361 gttgacggtc agcgacgtgt cgccgcggct cggcgtattg tggcggccgt cacgggactg
    21421 gcaggcctgg ggccaggtcg ccagcggctt ccgcgcgcct cccgccgagg acctgaacat
    21481 cgggctggac attccgctgt tcaggacccg tgccctgccg aaccccgacc tgaagtccga
    21541 aacgtcgctc ggctgggaag tcggcctgcg cacctgcggg cgcgcgccgg cgacgccgac
    21601 ccggcgctgg ccgcgttctg gaccgactac gaggacttca tcgagtcgct ggtgccgctc
    21661 gggccggacc cggccaccgg gatcctgttg ttccagtcgc agaaccggga tcgtgcgcgg
    21721 atccggggga tagaagtcga gggcggcatg ccgctcggcc agttgactcc ggcgctcgat
    21781 gcctttgccg caggcgtatc cgcctactgg gcggagggcg aggatcgtcg caccggcgag
    21841 gacctagtgg acgtgggtcc gccgtcggcg gtgctgttcg ttgactgggc cagcccttcc
    21901 gccgcctggg agatcaggtt gagcggaacc ttcgcgcgcg gcaagcaagg cgacgatccc
    21961 gccgggacgc cggacttcag cgtgccggga cacggcatcg tcgacctggt gaccgcctgg
    22021 cgcgccagcg agcggctcac gttgcgggca gggctgttca acgttaccga caggacctgg
    22081 tggcgctggg gcgacacccg cggcttgccg gccgacgacc cgctgatccc ggtgcggtcc
    22141 gcgccgggcc gcagcatcac cgtttcgttc aacttcggct tcgggccggg gcaatactga
    22201 attgcggtcc ctattcgggg ccgacacggg aggaaatgag atgaacagtc gagtattgat
    22261 gattacgctg ctggccggcc tcgcgatggc cggttgcggg ccgcgcgagc cgcagcaggc
    22321 ggcgccgccg cggacgggcc aggacccggc gacggccgtg cacgccgccg aggtggcttc
    22381 ggccggccag agtctggagc agcgcatgac ggccggtcgc aaggtttacc agaacgtctg
    22441 ccaggcctgc cacggtccgg agggcaaggg cctgccgggg gcgttcccgc cggtggcgaa
    22501 ctccgactgg atcgagcaga accccgacca gttgatcggc gcaatcctca acggcctgtc
    22561 cgggccgatc gtggtgaacg gccagaacta caacgccgtc atgccgccga tggcttacct
    22621 gagcgacgag gacgtggcca atgtgctgac cttcgtgctc aacagctggg acaatccggg
    22681 cggggagatc cagcctgccc aggtggcaca ggtacgcggc ggtgcgccgg cggccgccac
    22741 caccgcgccc ggcgagcatc cggtcccggg cacctcggcg ctcaagtacg agggcgcgcc
    22801 gctcggcatg gaccctgaga ccgcgcgccg gctggtggat tcggaagggc cgcccatcac
    22861 gcacgcggag tgggcccgct cgacggagct ctatttcgag cgctgtgccg gctgccatgg
    22921 tgtgctgcgc cacggcgcga ccggcaagcc gctgacgccc gacgtgacgc gcgagaaggg
    22981 caccgaatac ctgaaggcgc tgattaccta cgggtcgccc gcgggcatgc cgaactgggg
    23041 cacctccggc gacctgaccg aaggcgagat ccaggcgatg gcgcactttc tgcagcacga
    23101 gccgccggcg ccgccggagt tcggcatgga ggagatgcgc gcgacctgga aggtgctcgt
    23161 gcctccggag gcgcgtccgg acaagccgca gcacaatcgc aacatcgaca acttcttcgc
    23221 cgtgacgctg cgcgactccg gcgagatcgc catcatcgac ggcgacacca aggagatcgt
    23281 cgccgtcgtg ccgaccggct atgcagtaca catcacacgg ccgtcggcct cgggccgtta
    23341 cgcgatcacc atcggtcgtg acgccaggat cgacatgatc gacctgtggg ccgatccgcc
    23401 cgcccgggtc gcggaaatca agatcggcct cgaggcgcgc tcggtcgaaa cctccaagta
    23461 caagggctac gaggaccgct atgccatcgc gggcgcctac tggccgccac aatacgtggt
    23521 catggacggc gacaccctcg agccgctgaa gatcgtgtcg acccgcggca tgacggtgga
    23581 cacgcaggaa tatcatcccg aaccgcgcgt cgccgccatc gtgtcgtcgc acgagcaccc
    23641 ggagttcatc gtcaacgtca aggagaccgg ccagatcctg ctggtggact acagcaacgt
    23701 gaatgcgctc aagaccacca ccatcggcgc cgcccgcttc ctgcacgacg gtggctggga
    23761 tgccagcaag cgctatttcc tgacggccgc gaaccagtcc gacaagatcg ccatcgtcga
    23821 tgcccgcgag cagaagctcg aggcgctggt cgacgtcgag cgcattccgc acccgggccg
    23881 cggcgcgaac gtcatcgacc ccaagttcgg cccggtgtgg atcacctcgg cactgggcaa
    23941 tgccgatatt tcctttatcg ccaccgaccc ggcgaagcat ccggagcatg cctggaaggt
    24001 ggtgcgagtg ctgcagggcc agggcggcgg ctcgctgttc gtcaagaccc atccgaagtc
    24061 ccgcaacctc tgggtggaca cgcccctgca tcccgaggag aaggtcagcc agagcatcgc
    24121 ggtgttcaat atcgacgact tcgaggccgg ctacgaggtg ctcccgatcg cggattgggc
    24181 caacctgggc cccgggccga agcgcgtcgt gcagcccgag tacaacaagg ccggtgacga
    24241 ggtgtggttc tcggtgtgga acaccatgga cgcacgttcc gccatcgtgg tcgtggacga
    24301 caagacacgc aagctgaagc acgtcatcga cgacgcgcgc atcgtcacac cgactggcaa
    24361 gttcaacatc tacaacacca tgcacgacgt ctactagact tcaagtgcgc gggccccggc
    24421 tgcgcaagcg gccggggcct tttcctttgt gcgactccgg cgacgaactt gcggcgaata
    24481 ttctgtccaa gcaagaactg cagcgaggaa cacacatgaa acgacgcact gtgatcggcc
    24541 acgtggcggc cgccgcgatc ctgcccgctt ttttccccct ggtcatgcgc agcagcgccg
    24601 cgagtgaagc ggcgcccggc gatctgcggc aggtgagcca cgagcgctgg cgcgagctgc
    24661 tgcccgcgga ccgctaccgc gtgctgttcg aggagggtac cgagccggca cgcagcagcc
    24721 cgctggacaa gcgctacgac gacgggacat acatctgtgc agcttgctac cagccgctgt
    24781 tcgacagtgc ggacaagttc gacagcggca ccggctggcc gagcttcacc cgtccgctgg
    24841 agggcgcgat gggcaccaag cgcgacttcc ggctgatctg gccgcgcacc gagtaccact
    24901 gcagccgttg tggcgggcac cnnnnnnnnn ngttcaacga cggcccgccg ccgaccggcc
    24961 agcgctggtg caacaacggc ctggcgctgg agttcgtgcc gcgcggtgag ccgctgccgc
    25021 cgctgcggga cccttcgtga gcgcgcgggg ggcgtgtccg gctgcgcccc tggcgtcgct
    25081 gtgcctgttg tttctcggcc tggccgccca tccggacgcc gctgcggacg aaggcggcgg
    25141 tggggagggc cgggttacgg gtgaagcagg gctggcgctg gcgaccttcg ccggcggctg
    25201 tttctggtgc atggagccgc ctttcgacgc gctcgagggc gtcgtgtcga ccaccgccgg
    25261 ctacgcgggc ggcgcggagc gcgacccgac ctatcgccag gtcgcaagcg ggcagacggg
    25321 gcatgccgaa gtggtgcagg tgaccttcga tccgtcgcgg gtcagctatg ccgaactgct
    25381 cgaagtgttc tggcgcaata tcgatccgtt cgcagtggat cgccagttct gcgactccgg
    25441 ccctcagtat cgcagcgcca tcttccacca cgacgaattg cagcgcgcgg cggccgaagc
    25501 gtcgctggcg gcgatcgagg cacgcctcgg cgcggccgtg gcgacgcgca ttgaggccgt
    25561 aggggatacc ttccacgcgg ccgaggagta tcaccaggac tactacctga agaatccgct
    25621 gcgttaccgc tactaccgat acaactgcgg ccgcgaccgc cggcttgagc aggtgtgggg
    25681 ctcggaatct agtcgtgaag ataagtaaaa caagacaata ggctgcaaag ttaatgttag
    25741 ttatttgaag tttttcgggc cttgtcgcgc tcgtcggcag caccgcggcg gggcgatgca
    25801 agtgtggccg ctgactgcct tgtatacata catacatgta tgtattcttt acggcccgtc
    25861 ctgaccagcg gccacggccg atcgccggcc agcccgcccc cgcgccatcc aaatgggagc
    25921 gaccatacat gtgcgcctcg agagactcgc gttgttcacc gatcgcggcc tccgcggccc
    25981 tgctggcact gctggtgggg ctgaccggat gtggccggcc gcaggacggc agtacaggac
    26041 acggccaggg cggtggcatg ccgccggcgc cggtgacttt catgaccgcg gcggccgaga
    26101 ccgtgcagat cgagcaggac tacaccggcc ggctgcacgg ctggcgtgag gccgaagtgc
    26161 gcgcgcgcgt cggcggcatt ctcgaggagc gactctacga agaaggcgca caggtcgagg
    26221 agggcgcgcc gctgttccgt attgaacggc aggcctaccg catcgctctg caacgcgccg
    26281 aggccgaact ggcgaacgcg caagcggccc tcaaccaggc gcagcgcgaa tggcgccggg
    26341 tcgccgggct gcacgagcaa cgggcggtca gcgatcgcga acgcgaccag gcgctgtccg
    26401 caagggaact cgcggaagca aggctggccc tggcgcgcgc cggcgtgtcg caggcgcagc
    26461 tcgagctgga gtacacgacc gtgctggcgc cggtcggcgg tgtgagcggg ccggaggccg
    26521 tgacaccggg caacctgctg gcacccggtg cactcctgac cacggtgacg caactcgatc
    26581 ccatccagct gcgcttctcg ctgcccgtgg ccgacgccga ggcgcggcga cggctcgcgg
    26641 gcgacagtgc ggaatcgacg gccgacgtgc gcctggttct gccggacggc accgtctacg
    26701 aggagcgtgg ccacatcgat ttcaccgccg gtactgtcga cgcacgcacc ggcagcgtga
    26761 atgtgcgggc cgtgtttccc aacccggcgg gcagcctggc accgggcgcg ctggcgcgcg
    26821 tgcgtttccc ggtggaacga ctggagtccg tccacctggt ggactcggtg gccgtgttgc
    26881 agggaccggc cgggccggta gtgttcgtgg tcgatgaaga gaacaccgcg cgagcccgac
    26941 ccgtgaagct gggtccgatg atcggcaatc ggcgggcagt gctggacgga ctcgccgacg
    27001 gcgaccgcgt cgtcgtcaac ggccaggtcg cgctgcgcga cggggcgccg gtccagccgc
    27061 agccccgcga cgggcaggcc gactgatgct gcgattcttc atcgaccggc cgattttctc
    27121 ggcggtcatc tccatcgtga tcgtcatcgg cggtgccgcg gccctgcgcg cgctgccggt
    27181 ggaacagtat cctgacgtcg tgcccccaca ggtggtcgtc acggccgtct atcccggcgc
    27241 cagtgcgcag gtgctggcgg attcggtggc cgcgccgctg gagcaggaga tcaacggcgt
    27301 gccgggcatg atctacatgc agtcctcgag caccgactcg ggcgccgtgc agatcaccgt
    27361 gtcgttcgaa atcggcaccg atccggacca ggcgcagatc aacgtgaaca accgcgtgca
    27421 ggccgcgctg tcccgcctgc cgcagcgggt tcgcgacctg ggcgtgcgag tcgaagcgcg
    27481 ctccaccaac atcctgctgg tcgcggtgat gatctccccg gacggccgct tcgacaccct
    27541 ggccatcagc aactacgcgc tgctcaacgt gctcgacgaa ctcgtacgga taccgggcgt
    27601 cggggacgcg tcgttgttcg gcgcccagga ctattccatg cgggtctggc tgcagcctga
    27661 cgcgctggcc gcgtaccagc tcaccccgtc ggacgtcgcc gcggcggtgc gcgagcagaa
    27721 tgcgcagttc gccgccggca gcatcggcgc cgagcctgcg ccccggggcc aggatttcac
    27781 cttcgccgtg tccacccggg gccagctcag cacgccggag gagttcgagc agatcatcct
    27841 gcggtccgac gcggacggtg gcgtgttgcg gctcggcgac gtggcgcgag tcgagctcgg
    27901 cgccagggac tatgccttcg ccgcctccta cggcggccag ccggccgtac cgatgggggt
    27961 gttcctgcag ccgggtgcaa actcgctgga gaccgccgac gcggtacgcg cggccttcga
    28021 ggaaatgtcg gcaaggttcc cggagggtct cgagtaccgg atcccctacg acaccagcgt
    28081 attcgtggac atctccatcc gcgaggtcta cgtcacgctg atcgtcgcgg tgctgctggt
    28141 ggtgctggtg accttcgtgt tcctgcagca cctgcgggcg acgctgattc cggtggccgc
    28201 cattcccgtc tcgctgatcg gtaccttcct gggcatgcag gctttcggct tctcggtgaa
    28261 cctgctgacg ctgttcggcc tggtgctgtc gatcggtatc gtggtggaca acgccatcat
    28321 cgtcatggag aacgtcgagc gcctgatgac cgagcaggcg atgaaggcac gcgaggccgc
    28381 gatcgaaacc atgcagcagg tggcgggcgc ggtggtgtcc tcgacgctgg tgctggtcgc
    28441 ggtgttcgtg ccggtgacgt ttctcggcgg gctgtcgggc gagctgtacc ggcagttcgc
    28501 ggtaaccatc gcgatctcgg tggtggtgtc cggcatcgtc gcgctgacac tgacgcccgc
    28561 gatgtgcgcc ctgctgctcg acaaggaggc caggacggtg gccgcaccgt tccgggtgtt
    28621 caaccgcgga ttcgagcggg tcacgggcgg cttcgtcgcc ggcgtcgctt tcttgctcaa
    28681 gcatgcggtt ctcggcgtgg tgctgttcgc gggcgtcatc gcgctggcgg gcgtgctgct
    28741 ggcgcggttg ccgagcggtc tggtgccgca ggaagaccag ggcgtggcgc tggtggtggg
    28801 gctgctgccc gccacctcgt cgctgcagcg cacggaggag gtgcgcgacg tcctgaccag
    28861 cggcttgctc gagttcgagg agatccagga attcacttcg ttcgccggct tcgacatcat
    28921 cgccggctcg ctgcggacca acgcgatcgt cggcttcgcc aagctcaccg actggcggga
    28981 gcgcgaaggc gaggggcagg acgcagcctc ggtgaccggc aggatcatgg ggctgggctt
    29041 cggcatccag gaagccaacg tgttcgcctt caccccgccg ccgatccagg gcctgtcgct
    29101 caccggcggc gtcgagggct acctgcagtc gcgcggcgag acctcggcgg gggcgctgga
    29161 gtcgctggtg ggacgtctca gggcggccgt ggcggagcgg ccggagatcg gcagcctgca
    29221 ggtgccgttc gacgccaacg tggcacgtta ccgggcggac atcgaccggg accaggtgcg
    29281 tgcgctcggc gtgccgatca acgcggtctt cgacgccatg cagagcactt tcggcgcgct
    29341 gtacgtcaac gacttcacgc tcatgggccg caactggcag gtcaacctgc agtccgaggg
    29401 cgagtttcgc agccgaccgg aggatctgcg gcgcgtctac gtgcgctcgg cgcagggcga
    29461 gatggtgccg ttgagttccc tggtgcggct ggtgcgcacc ggcgacaccg acgccgtgga
    29521 ccgttacaac ctgttcgtct ccgcgcgggt gctggccgag ccggcacccg gctacaccag
    29581 cggccaggtc aaggcggcgc tcgaggcggc ggtggagcag gcgctgggca ctgacgcgcg
    29641 catcggctgg atcggcgagg cctaccaact ggaggctgcc gcgggtgcgg gtacggccgc
    29701 cttcgccatg gggctgatca tggtgttcct gatcctggcc gcgcagtacg agcgctggac
    29761 cctgccgctg gcagtggcga cggcagttcc cttcggcgtg ctcggcgcgg cgctcgcagc
    29821 aatgctgcgt ggttacccga acgacattta cctgcaggtc ggcctgctgg tgctgatcgg
    29881 cctggcggcg aagaacgcca tcctgatcgt cgagttcgcc gcccagaatc gcgccgccgg
    29941 catgagcgcg ctcgaggccg cgacggcggc ggccaggcag cgtttccgcg ccatcatgat
    30001 gaccgccctg accttcatca tcggctcgct gccactggtg ttcgcgaccg gcgcgggcgc
    30061 agccagccgc cgcgagatcg gcaccgtagt ggtcggtggc atgatcgcgg ccagcacact
    30121 ggcgctgctg ttcgtaccgc tgttcttcaa gttgctggag gacctttcgg cctggcgaag
    30181 gcagcggcgc ggcggggagg aggcgagcca tggttaaggc ggccatggtc ttgctggcgg
    30241 cggcggcgct ggccggttgc gcggtcggac ccgattacca ggcgccgcag ctggaactgc
    30301 ccgagcactg gccggacaac gtcgtgctgg accccgcttc cggcgagctg ctcgccgcct
    30361 ggtggcggcg gttcgaggac ccggtgctgg acgggctgat cgagcgcgca ctggccgaca
    30421 acctgcaggt gcgcatgcag gcggcgcgcg tcgaggaagc gcgagccagg ctggggctcg
    30481 cgcgtgcgga acagtggccg accgtgggtc tgcaggccga ggctgcgcgc cagcgccagc
    30541 cggcggcgac gttcggtatt gcgggtctgg agactccgcc gcgcaacctg ttctcgatct
    30601 cgggcgtgct gggctacgag atcgatctat ggggccgcct ggcgcgcgag cgcgaggcgg
    30661 cgatggcgcg gttgcagggt agcctgtatg cgcaagaggc ggtgcggctc ggcgtcattg
    30721 cggacgtcgc ggtgggctat ttcaacctgc gcagcgcgca gcgacagctg gacgtgaccg
    30781 agcggacact ggccgcgcgg ctggaaggcg tgcgcatcga gcggctgcgt ttcgaggcgg
    30841 gacagatcga cgagctggcg tatcgcgagg ccgagtcaca gctggcgggc acccgtgccg
    30901 agctgcccgg gcgcatcagc gagctgcggc gacgcgagag cgcgctgggg ctgttgctgg
    30961 gcctgtcgcc cgaggagctg ctcggtgcgc tgcaggtcga cgcggggacg ctcggcgaga
    31021 tcacccttcc ggccggcatt ccggcgcacc tgccgtccac gttactggtg cggcggccgg
    31081 acgtgcgcgc cgcggaggcg gaactggcgg ccgccacggc gggcatcggt gtcgcccagg
    31141 cggcccgcct gccccagttc aacctggccg gcctgctggg cacggcggcc ggcagcgccg
    31201 gtgacctgtt cggggacgag gcgcaggcat ggagcatcgg cacgagcatc ggggtgccat
    31261 tgctcgattt cggtcgcggc aggacgcagg tcgagacggc cgaagcgcag cgcctgcagg
    31321 ccgaaatggc ctaccggtcg acggtagcga acgccttcgt cgaggtgcgc gacgcgctgg
    31381 tgttctacga gagcagcggt gcgcggctgg cggcgatcga cgcccaggtg caggccctga
    31441 agcgcaccga ggagctggcg ggcattcgct accgggacgg ctacgtcagc atcatcgaat
    31501 tgttggcggc gcagcgcgcg ttgctcgccg cggaactcgc gctcgcgcag gccgaagccg
    31561 atcgtctcgc cgctgcggcc accctgttca aggcgctggg cgggggctgg gaggcgaagt
    31621 ggtgaggcgt acccgggagg atgcgcaggt cacgcgcgag gcgattctcg acgccgcgca
    31681 gcgcgtgttc ctggcacgcg gcgtgtcgcg caccagcctc gagcagatcg cacgcgaggc
    31741 cggggtcacg cgcggcgcga tctactggca cttcaaggac aagagcgacc tgttctcggc
    31801 catgctcgag cgcgtccgcc tgccgctgtc cgatctcgcc gatgcctacc ggcagggcgg
    31861 cggccgccgc gacccgctcg gcatgctgga gaagctctgt ggactggcat tgaccaagct
    31921 cgacgcaagc gagacttatc gcaaggtgta catcatcctg atgacgcgct gcgagttcgc
    31981 cggcgagatg aacccggcct tcgagcgaca gctggagatc gaccgcgaca acctgcgcaa
    32041 ggtggagcag gacttcgtcg aggctcgtga gctcggccag ctcgcgccac acgtggaccc
    32101 gcgcatcgcc acgctgacgc tgtactcgct gatgcacggc atctaccagt cctggctgcg
    32161 caccccacag agctacgata tccgcacgga cggcgctgcc atgctgcggc tgttcttcac
    32221 gggactgcgc gccaccggcc actgagaggg cggcggcgcg gctggacttc gacctgcggc
    32281 ccggcttatc gtcccgcgat gctcgccaac ctcggtctgc tgatttcgct cggattgctc
    32341 atctggctgg tgctgcgcgg cgtgaacgtg attctcgcct cgctgctcgc ggcgatcatg
    32401 gtggcgctga gcaacggcct gccgctggcc gagagcctgc tgcagcatta cgccaccggg
    32461 cgctccggcg ccttcacgtt tgcagggcaa tttttcctga tttttttcgc cggcgcaatt
    32521 ttcggcaaag tgatggccga gggccgggcg accaccagca tcgcgctgct gttccgtgac
    32581 ctgctgggac gcgagcgggt gctgtggatc gtgatgctgg cctgcgctct gctgacgtac
    32641 ggcggggtgg tggtgttcgt ggtgattttc gccgtctacc cgctggcgct ggacctggcg
    32701 cgcgacgcca acacgcccaa gcgcctgctg gcgggcgccg cggcgctcgg cgccggcacc
    32761 ttcaccatga cggccttgcc cggcacacct tcgatccaca acgtcatcgc ggcgcgttcc
    32821 ctcggcaccg acctgttcgc cgcgccactg ctcggggtcg tggccgcctt gctcatgatg
    32881 acgcttggca tggcctatct cgagcgccag cggcggcgcg cgctggccag cggcgagggc
    32941 ttcgatcctg ccccgcacga acacgtgccc gacgatgccg aaatcgagca ggggctgccg
    33001 cactggggcg ttgcgctgct gccgttgctg gtagtgctcg ccatcatcat cgggccgcga
    33061 ctcatcggtg tgttcgttac gcccggcggt gaatcgacgt tggcgcgatt ggtggccttc
    33121 gccgccggcc agccggtgtt ctggccaagc cttgccctgg cctgcggtac ggcactggcg
    33181 ttgctgctgt tgccacgcct cggcggccag cgcatggcca gcctcggacg cggcaccaac
    33241 gaggcgatcc tgccgatcat gaacaccgca gcgatcatcg gcttcggcgg tgtggtcatc
    33301 cagaccatcg gcttccggca gttcagcggc ctggtggtgg agaccggcct gccgccgctg
    33361 ctgtcggcct ttctctccat cagcacggtg gcggggatca ccggctcggc ctccggtgga
    33421 ctgcagatct tcatggcctc gctggcaccg cgttatctcg agatgggcat cgaaccggaa
    33481 accctgcatc gcatcgccac gctggcctcc ggcggcttcg attccctgcc gcactgtggc
    33541 gccgtggtgg tgatgatgac catcatggga ctgtcgtacc gcacggccta ccgcgacatc
    33601 gcgatcgtca ccatcgtggt gccggtgctg gcgacgctgg cggcgatggc actcgccggc
    33661 gtcgccggct gagcgcggct caggtgtcgg gcgcctcggc gctgatgttg cggcaagctg
    33721 tctcgaccag gaatcgcgag gcgatcgcgg ccagcccggc gaagcccgcg agcagccaca
    33781 ggccgcgctg gtagtccccg gccgcgtaca ccggcacgcc gtccagcatc cggccgtccc
    33841 aacctagatc catggcccag ccgaacagcg gctggaacag cgcggtgccg agaaacaggc
    33901 cggtgttgac gagtgcgatc gccatgccgg acagcgccgg cgccgccact tccttggcgt
    33961 gcgcataggc gaccacgaag ctgcccgcgc tgaggcccaa cagggcaaac agcgcgaatg
    34021 cgaacgggcc cggccgccag ggcaggaaca gcaggccgag gcagacggcg aagtagacca
    34081 gtgcgccggc ccgtagcacc ggctggcggc gcagcatgcg atccgagagg ctgccggccg
    34141 ccaggcagcc gagcgcgaag gcgatggtgg ccagggtggt gtagagcgag gcttcaccgc
    34201 ggctcagtcc gtgaacgtcc cgcagcaatg gcacggccca caggccgatc atggcgaaca
    34261 ggctgccggt catgccgaag tcgtaggcaa atccgggcca caggcgacga ttggccagca
    34321 ccgccctgag gtcgtgaacc cagtggcgcg tgcgcggggc gtgcgcggcc cggccttcca
    34381 tctggcgtac cgagggaaat ccgagatcct cgggcttgtc gcgcaccagc caccacgaga
    34441 ccagccccag ccccatggcg aacacgccga gcgccacgaa cagcatgcgc cagtcgacgt
    34501 gcaacagcat cagcgccagc gggccggtgg cggcgaccgc gccgaggttg cccagcagca
    34561 gggtcaggcc gctgatcgtc ccgtagcgcc gctcgctgaa ccagaccgta ttgctcttca
    34621 tcaggccgac gaacaccacg gatacaccga gacctaccaa gaatcggccg atgctcgccg
    34681 tggcaaaggt ctccgccagg ccgaagacga ccgagccggc gccggcgacg acgttgccca
    34741 gcgtgaccgc gaagcgtgag cccagcgtgt cggcgagcac gccggcggga atctgcatca
    34801 ccgtgtagat gtagaaatac atcgccgcga gcgatccgag ggcagcggcg ctgatgccga
    34861 aggcctgggc cagctccgcc gagaccatgg ccggcgcaaa gcggtggaaa aaggccagca
    34921 tgtaggccgc gaccagtacg ccgtagattg tccagcgcag tcgctcgaag cgagcgtgat
    34981 cgtggtcgtt catcctggct ccgatcccga attgcgcaag gcacgcgcac agattgcgaa
    35041 gactaagcca aattcgagtt gctgccgagc cttcaggaca catcgcggct tcgcacttga
    35101 atgcccggcg gggcggggcg cacaatccga atgccgggcg agcacgcctg caagggaccg
    35161 gggcgaacct gggggaatcg ccatgaacga caccacgccg ggactgctgg tcgtcgatga
    35221 cgacgaggtc gtgcgcgaaa tgctgcggga ttacttctcc cgtcagggct ttgaagtccg
    35281 gctggccggt tcggcggcgg aggcgcgcag catgctcgat gcggggactc cgcagctggt
    35341 gttcctggac gtgggcctgc ccggcgagga cggcctcagc ctggcccgcc acatccgcga
    35401 gaagctggac atacccatcg ttatgatctc tggtgccggc gaagccctcg accggatcat
    35461 cggcctcgag gtgggcgcgg atgactacgt caccaagccc ttcgacccgc gggaactgct
    35521 ggcgcgcgtc aagagcatca tgcgccgtta ccagcgcgcg cccgaggcgc tcgaggagcg
    35581 cccggcggag cgagtgagga ttcccggctt cgagctggat ctggcgtcgc ggcggctgtt
    35641 tcgcgcggat ggtgaggagc tcattcttac gcgcatggaa ttcgacttgt tgcaggtttt
    35701 cgtcgagcat cccaatcgcg tgctgtcgcg tgaccagatc ctgaacatga cccagaaccg
    35761 tgactgggac ccgttcgacc gcagcatcga catccgggtg gcccggctgc gccgcaagct
    35821 ggaggactcg ccggacagtc ccgtgctcat ccgcacggtg cgcggcgtcg gctacatgtt
    35881 cgtgcccgac gcggcctgag gccgtcgccg cgtcaccggc ggagccgcca atgtttccgc
    35941 agcgtttcga ttgttgccgg attgtatccg cacgttgtgc cacgacccgg gagcggtaga
    36001 ctcgaatcat ggccgccgaa cgccccgccc gtcccggtaa ttccaaatcg tccgcagtgc
    36061 agccgctgcc ggccgaggcg ctgcggcagc tgctcgacgc aacgtccgca attgtggcgg
    36121 tcaaggacct cgagggccgc tacctgcacg tcaaccgcgc ctttcttgtg ctgctcggcg
    36181 gcgaggagcg cgactacctc gggcacacgg accagcagat cttcagcccg gcgatggccg
    36241 cgcagctgcg cgccaacgac cagcgggtgc tggcggagcg gcgcgagctg gtattcgagg
    36301 aggatctcga agtcgacggc cggctgcaca gttacaacac gcgcaagtat cccttgctgg
    36361 acgccgaggg ccggccctgg gcggtgtgcc tgacggcgga agacgtcacc gacgccaggc
    36421 gcaccgacca ggcgatgcgc cgcgtggcgc tcgggatctc ggcggccagc ggcacggagg
    36481 tgttcgagct cacggtggcc agcgtggcgg cggtgctcgg cgtcgatctc gccttcgtcg
    36541 cggcgttcga ggaggacagc ggcaagcgcc gtttgtcggc gctcgccatg tgcgaggaag
    36601 gccaggtagg cggaaactat tgttacgaga tccgtggcac accgtgcgaa atggtgctgg
    36661 cccacggctt cgagtactac tcggagaacg ccgccgagat gttcaccggc gacccggacc
    36721 tgagggcggc gggctacgtc agctatgcgg ggtatccgtt gatggatgcc gacggcgagg
    36781 ccgccggcgt attgtcggtg tgccaccgcg ggccgctgcc gcccgtcggc atggtgcagt
    36841 ccgtgctcgc gatcttcgcc gtgcgcacgg cgtcggagct cgagcgcgtg cgcatggacc
    36901 gggcattgcg cgactcggag cagagctacc gcaccatatt cgaggcgtcg caggactgca
    36961 tcttcattca cgacgtcgac accggtgcac tggtcgacgt caatccgcgg gtctgcgaaa
    37021 cctacggcta cgagtacgac gagatcctga aacttggccc gggcgacctc ggctccgggg
    37081 aacccccgta cacccaggcg gatgccgagg ccgccatgcg gcgtgccagg agcggcgccg
    37141 tcgtcaaggt cgagtggcac cgccgcaatg ccgacggcac tcttgcctgg gacgaggtcg
    37201 tgctcaagcg cgtgcggctg gccggcgtcg accgcatcat ggccgtgacg cgggacatca
    37261 cggagcggaa ggagagggaa gaggcgctgt cgcgcagcga agaccggctg cgggccacgg
    37321 tcgaggccgc gctggactgc atcatcacca tcgacagcgg cgggcggatc agggagttca
    37381 acccggccgc ggagcggtgc ttcggctatc gtcgtgagga cacgctcgga cgcccgctgg
    37441 ccgagctgat cgtgccgcca cggttgcgcc aggcgcacca gcgcggcatg gaaaggtatc
    37501 tgatgggcgg cgagtcgcgc atgctcgggc ggcgcgtcga actgacggcc atgcgcgccg
    37561 acggcagcga atttccggtc gagctcgcca tcgccgaggc gcagggcgtg gacggcgtag
    37621 tattcgtcgg ctacctgcgc gacatcactt tgcagcgcga ggccgagcgg gagcgtgccg
    37681 ggctggaggc ccagctgcgc caggcgcaga agatggaggc gatcggccat ctcaccggcg
    37741 gagtcgccca cgacttcaac aatatcctca ccggcatcat ggggtatctc ggcatggccg
    37801 acgacattgc ccgcgagctg gagcatgcca ggttgcagca atacctggcg cgtgcccgcc
    37861 actcgggcac gcgcgccgcc gacctgatcc agcagatgct cactttcagt cgcgggcgcc
    37921 gcggtgatgc gcgggcgatg gcgcctgcgc cgctggtggc cgacgccctg cggctggtgc
    37981 gcgcaaccct gccggccacc gtagtgctgg cccgcaagct ggacgacgat gtcccgggca
    38041 tcatgttcga tgcggtgcaa ctcgagcaga tcctggtcaa cctgtgcatc aatgcacggg
    38101 atgccatggg cggacagggt tcgctggagg tcagcctgcg cgggatcgag gtcgaggccg
    38161 cagtatgcac ctcgtgtcag caggcattcc gcggccgcca cgtggtgctg tcggtcgagg
    38221 acaccggtcc gggcgtgccg gacgaggtgc tgcacaggat gttcgagccg tttttcacca
    38281 ccaaggcgcc ggggcagggc agcggcatgg ggctggcgac cgtacacggc atcgtccatg
    38341 agcatggcgg acacatcgtc gtcgcgaatc gcagtggcgg cggcgccgcc ttccatgtgc
    38401 tgttgccgcc gctcgaccat gcggcagacc ctgccggggc tgaccccggg gagggtcgtg
    38461 cggagccgga ggcggtcgcg gcgctgagtg gcagggtcct gctggtggac gatgacgaga
    38521 ccgcgcggga attcatgacg gacttgctgg gcgaatgggg cctggccgtg acggccctgg
    38581 ccggcccgct cgaggctcgc gctgtggatg ccggtggggc gcgattcgac gtcgccatcc
    38641 tcgaccacac catgccgcac atgacgggcc tggaactggc tcgccggttg cacgctgagg
    38701 accgggaact gccggtgctc ctgtatagcg ggtacgcgga acagttggcg gccggagagc
    38761 tcgcggccgc cggcattcgc gcgcagttgt cgaagccggt cgatacggcc gccttgttca
    38821 agcatctcga gacgctgctg gcgagacgct gaccccggcc cgccgcgcgc cgcgcggaaa
    38881 catttctgtt acgcagcgat acgcggcgga catcgttccc atacaacggt acttcattct
    38941 tcctccatgt ggggcggcca ccacgccgcg ccgccagggc aagggaggaa gtcatcgtgg
    39001 cgatctacca aagcgtgctg gatacggtcg gtcggacgcc cgtggtgagg cttcaccggc
    39061 tcgcgcccga gggtgtagag ctgtacgtga agctggaaag cgccaatccg atgggctccg
    39121 tgaaggatcg ggcggcgctg gcgatcatcg aacaggccga ggccgacggc acgctgcagc
    39181 ccgggcagac ggtggtcgag gccaccagcg gcaacaccgg tatttcactg gcgatggtct
    39241 gtgcccgcaa gggttacccg ctggtcgtcg tgatggccga gaacttcagc gtcgagcgcc
    39301 gccgcctgat gcgcttcatg ggggcgcagg tggtgctcac gccggccgcc gaaaagggca
    39361 gcgggatgtt ggccaaggcg gtggaactgg cccggacgca tggctggttc ctgtgtcgcc
    39421 agttcgaaaa cgaggccaat gccgacgtgc acgcgcatac cacggcgcag gaaatcctgg
    39481 cggacttcgc cgcttccgga ctggactact gggtcaccgg cgccggcacc ggcggcacac
    39541 tgagcggcgt tgcccgtgcg ctgcgggagc acagcccagg cacgcgggtc gtcgtctgcg
    39601 aacccgacaa cgcgccgatg ctcggcagcg gtatcccgca gcccgacagc gcggcgccgg
    39661 accgggccgc cagccacccg atgttccggc cgcacctcat gcagggctgg agcccggatt
    39721 tcatccctga gctgacccag cgcgccatca cgtccggcct ggtcgacgag ttccagccgg
    39781 tgaacggcgc cgaggccctg gccacggcgc ggctgctggc tgcgcaggaa ggcattctcg
    39841 ccggcacttc cggcggtgcc acgctggccg gcgcgctggc gctttgcaag cgcgtccctg
    39901 ccggatcgcg ggtgctgtgc atgttgcccg acacgggcga gcgctacctc agcacgccgc
    39961 tgttcgccga tgtcgccgcc gacatggacg aggcggagag gttgatctcc gcgtcgacgc
    40021 ccggcttccg cttcgacctg ccggcggcgc aggtgcccac ggctgcggcg gcgccgcccg
    40081 agaccgatgc ggagaccgag cgctacgtcg ccggcgtgtt caccgatccg cag
//
LOCUS       DAOMPG010000002        34915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_552_length_34915_cov_4.605020, whole genome shotgun sequence.
ACCESSION   DAOMPG010000002 DAOMPG010000000
VERSION     DAOMPG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 34915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34915
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_552_length_34915_cov_4.605020"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..746)
                     /locus_tag="PLS34_00155"
     CDS             complement(138..746)
                     /locus_tag="PLS34_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRP85913.1"
                     /translation="MNGTFSGRRFIRALLLWLVAIWIIAAIAPYSRRDWLLENLLVFV
                     FAATLLLTARRFTFSNFSYGLLGLFLTLHLVGAHYTYSEVPPGFWLQDALDLSRNHYD
                     RFVHFCFGLLVAPPIRELLVRAAGTRSGWSYPVATLSILGFSGFYEALEGIVAMIVSP
                     ELGAAYLGTQGDEWDAQKDTALAFAGAVVAMLVSWRLAKRPR"
     gene            868..1983
                     /locus_tag="PLS34_00160"
     CDS             868..1983
                     /locus_tag="PLS34_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018632161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP85914.1"
                     /translation="MQIPGRLAPLLIGLIAAVGATPAGASDSRPGGSLFIYNWSDYIA
                     EDTIAQFEAETGIKVTYDVFDSNEVLEAKLLAGSTGYDLVVPSLAFLGRQIIAGVFQP
                     IDRSKLSNYDKLDPQLLARIAEVDPDNKHAVPYLWGTTGIGYNVEKIAAIFGEDFEVN
                     SWDLVFDPAYAEKISACGFSMLDAASEMLPIALHYIGEDPNSFDPAVIAKSYDVLAAV
                     RPYVTYFHSSQYMNDLANGDICVAVGWSGDVFQAIDRADEAERGVEIDYVIPAEGAPV
                     WFDMLAIPADSRNVENAHRFIDFLLRPEVMAAITEYVAYANPVPASRELMDEELRDDP
                     DIFPPPEVMARLYGLKVLPRDVDRVYTRTWTRVRTGR"
     gene            2029..3189
                     /gene="potA"
                     /locus_tag="PLS34_00165"
     CDS             2029..3189
                     /gene="potA"
                     /locus_tag="PLS34_00165"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HRP85915.1"
                     /translation="MSNGQKPAGAARAAVSPARAEALGRQEYLKIEDVSKDFDGFVAV
                     DRVTLSIRQREIFALLGASGCGKSTLLRLLAGFEAPSSGHIRLDGQLLDGLPPYRRPV
                     NMMFQSYALFPHMTVADNIAFGLKQDGLSREERKHRVDEMLALVKMESMARRKPHQLS
                     GGQQQRVALARSLAKRPKLLLLDEPMGALDKKLRSEMQLEVVNIIEQLGVTCVMVTHD
                     QEEAMTMAHRIAIMDQGGIVQVGTPDEIYEQPATRFAAAFVGSVNLIEGKIEVDAPDH
                     ITIRATGLPEPIRVGHGHTGYEGMDVIFALRPEKLVISKEPPDQSNNRYQGKIEEIAY
                     LGSHSVMHIRLPSGLKLLAHVPNSRRWASEDFTWEDQVWASWDDMAGVVLSS"
     gene            3189..4094
                     /locus_tag="PLS34_00170"
     CDS             3189..4094
                     /locus_tag="PLS34_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP85916.1"
                     /translation="MKRSGLRLPGTRWLVIAVPFLWMLVFFAVPFAIVLKISFAEGLI
                     AMPPYSDLVTRTDTGITISLNLENYLYMVRDSLYVSAYLGSLKIAVVSTLLCLLIGYP
                     MAYAISRLDPAARNLALLLVILPSWTAFLIRVYAWIGILKNNGVINNFLVWIGAIGEP
                     LQLLHTPLAVYIGVVYMYLPFMILPLYASLVRLDPRHEEAAQDLGARPWRVFVSVTLP
                     RTLAGIIAGSMLVMIPVIGEFVIPELLGGPDTLMIGKVLWEEFFNNRDWPVASAVAIV
                     MLLLLIVPITIFHRFQSREMEKRLA"
     gene            4091..4927
                     /locus_tag="PLS34_00175"
     CDS             4091..4927
                     /locus_tag="PLS34_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP85917.1"
                     /translation="MRRVGRNSPFTLAVLGLGFVFLYAPILSLVIYSFNESRLVTVWS
                     GFSLKWYGELFADRQLMDAVWISLRVAFQTAWSAVAIGTMAAMLLVRFRRFRGKTLFG
                     AMITAPLVMPEVITGLSLLLLFVHIGELLGTPIMRGMGTIWIAHTTFATAFVTVVVAS
                     RLTEMDRSLEEAAMDLGANRLKVFFVITLPLIVPALVAGWLLAFTLSLDDLVIASFVS
                     GPASTTLPMKVFSSVRMGVSPKINALATLWIAAVSLAAIVAWRLMARGERRRRRELRA
                     AE"
     gene            4975..5703
                     /locus_tag="PLS34_00180"
     CDS             4975..5703
                     /locus_tag="PLS34_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRP85918.1"
                     /translation="MWKKFAPAMLAGFALCLLFVNAHADEDEGWWFGGSLAATSDYVF
                     RGVSQTDEGPALQGSLDVGHSSGLYAGIWASNVDFDSPDGIDLELNFYLGWAFEFDNG
                     TGLDLQLVRYQYPSANPGYGINYNELIISYSFLESYTATVAYSDDFLRSGESALYYHL
                     GADFPLGVAELNLTLGAGLNDISNVTGSDYWDYQVGINRDFGIVNADLSYFDTSGFDA
                     DVQDWLGPPNWADGRIVLTLSVEF"
     gene            5727..7259
                     /gene="ilvA"
                     /locus_tag="PLS34_00185"
     CDS             5727..7259
                     /gene="ilvA"
                     /locus_tag="PLS34_00185"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HRP85919.1"
                     /translation="MLQSYVKKILSSRVYDVAVETPLQAAPGLSRRSGCTVMLKREDL
                     QPVFSFKIRGAYNKIAQLPEAERARGVITASAGNHAQGVALAARELGLKSTIVMGRNT
                     PSIKVDAVRALGARVVLHGDTYDDAAAHAAELVARHGLVYVPPYDDLDVIAGQGTVGM
                     EILKQHAGAPTAILVPVGGGGLVAGIAAYVKYLSPGTRMIGVEAEGSACLAAALAAGR
                     RVTLPYESLDLFADGVSVAQVGREPFKVARRCVDEVIVANTDEICAAIKDLFEDTRSI
                     AEPAGALAVAGLKKWAAREAEAGRLAPAASVVAIVSGANVNFDRLRHIAERAEVGEHR
                     EAILAVTIPEKPGSFRRFCAVIGKRSVSEFNYRYADPSQAHVYVGLKIGAPEDRETVK
                     TLLDAAGYGVVDMTENEAAKLHVRHMVGGRPAGDKPELLFRFEFPERPGALLNFLSGL
                     GAEWNITLFHYRNHGAAWGRVLVGFEAERRDRTRLVGDLKRIGYRFWEESENPAYRLF
                     LR"
     gene            7368..7970
                     /locus_tag="PLS34_00190"
     CDS             7368..7970
                     /locus_tag="PLS34_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85920.1"
                     /translation="MTSNSEYRGSTSTSGQDDAARRRALRPGRLPTAVQVPVLLTGLA
                     TGLLVLMISSVLDARLVVAGAWFMVAYCGLVLLSLHPPPFFSAQLDRFLDEWVEDLGV
                     GYYAVAALAFFIPRQIQTTLSGDFSSFVIEWILKFSMETFFNVFHAMTWPFLLINRHG
                     MWPALGWVVATYGVFWIGTRAFSTPAWMLEKDDRDEAEAN"
     gene            complement(8049..9707)
                     /locus_tag="PLS34_00195"
     CDS             complement(8049..9707)
                     /locus_tag="PLS34_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRP85921.1"
                     /translation="MRLKFPLAFMPLVFCVAACSESGPGNGTPAIEATADATPPVELT
                     RYATVPLRADLTSFSDDERRALELMIRAAEIMDGLFWYGAYGDRDSLLASIEDPALLD
                     FARINYGPWNRLDGNRPFVPGYGDKPLGANFYPPDMSREEFDAWDQPGKTGLYSYVRR
                     DDAGRLVLVPYSVMHRQELQQAAGLLRDAATLVGNDALRRYLELRAAALVTDEYQPSD
                     MAWMDMKDNRIELVIGPIETYEDRMFGYRASFEAFVLVKDLTWSERLARFAEFLPELQ
                     RGLPVPAAYKAETPGSDADLNAYDAIYYAGDANAGSKTIAINLPNDEQVQLEKGTRRL
                     QIRNSMRAKFDQILVPIARDLIAEDLQRHITFNAFFSNVMFHEVAHGLGIKNTLDGRG
                     TVREAMREHGSALEEAKADVLGLYMITELFERGELHEGELLDHYVTFLAGLFRSVRFG
                     ASSAHGRANMIQFNDFSERGAFARGADGRYRVDLERMQEAVRVLAGRILVLQGDGDHA
                     GAGALLASHGVIRPELAEDLARINAAGIPVDVVFEQGLEVAGLR"
     gene            9876..10748
                     /locus_tag="PLS34_00200"
     CDS             9876..10748
                     /locus_tag="PLS34_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP85922.1"
                     /translation="MRIRRALKWFGIGLLGIAGLAASVVLGIYVAMGRDLGRSFAVSG
                     EEVFVPTDAASIEEGRRLAQLRGCTVGCHGLASGGAVMVELFDGSRVVAPDLGRIAAG
                     YSTVELERAIRHGVRPDGTSVIRIMPVEMFSGLSDRDLGLIVAYLRSLPPGAQELPDS
                     RYGPLARIMGFMFAREFGSVLAAEVTDHVHPPPPDMEDPAAAGRYLAQTVCTECHGDD
                     LRGAPDGSTPSLAAAAAYSRDQFETLMRTGEPLGDRELGLMGGVARSRFVGFTDEEIT
                     MLHDYLRNPATWAE"
     gene            complement(10765..12177)
                     /locus_tag="PLS34_00205"
     CDS             complement(10765..12177)
                     /locus_tag="PLS34_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRP85923.1"
                     /translation="MTDPAKPDSRQAAARLQQLWQLLQDGRRSHAIALAAALLDVKVD
                     DARYFAALGHALHSLQQFDAARLALQCAISLQPGAASLHLGLANLELALGNLGAAGAA
                     CERSLALQPGYPDALFFRSGLRRQTHDDNHIEELKAALGSPPTAPDAHARIQFALAKE
                     LEDLGRYDQSFPILLRGARLYRGTLQFDLSDEVRFLEAIGTTWNAAEVRRRDAAAPAS
                     ERVPVFITGLPRTGTTLVERILGAHSAVHSAGELPDFVRCLNRAMERLPGLEGHSRAA
                     MVPASTGLDFAALGRDYLELAQPLAGTTPWFIDKLPQNSIYLGLIHRALPEARLVLVR
                     RHPMDACYSMFKQIFTDSFAYSYDLDELAGYFIAHERLMRHWIAVLGDDLHVVRYEDV
                     VDRLEDEARRLLAFCGPPWEPACLEFHRNSAPSTTASAGQVRQAIYRSSVGKWRHFRA
                     ALQPLEARLRAAGCLDGWEP"
     gene            12303..13682
                     /locus_tag="PLS34_00210"
     CDS             12303..13682
                     /locus_tag="PLS34_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85924.1"
                     /translation="MKLGVENLHASAPARSTRAARPLRLAVAALVAGALGACGGNGDE
                     TGPTIVDPVILDPVADAQDESNDRIEITNTQSALDIRVIYPETPIPVDADGAGYGSEA
                     PVAMERIDFDQLASVTLTLASEISPPVVDNQVVQATAIAQSIGNNAVVSYNMRGEPRL
                     GAIDWVMRLNSARPALGSSATFNDADVSAVSTDGRNVYMAMASGAPEFPFPAVVERLR
                     VEGNRLTLAGNERAPLTSFVATSVVAVGDTVYATSGNTGGVFALAEDDLGRLGEFPLH
                     DARWVASDPEGGRIAVVQGTPGQVSVFAGGEFAGGGMTLLNTFPFSGADVPESKSTVE
                     VAGNKAFIAAGPEGVQVMCLDDGEIVGHVPRPDPAQLGLDSSVVVSNAVTVDGDLMFI
                     SNGEAGVYVARAAQPFASSDCSPQDITVIGKLRFDELQSANHVSFRGDYLFVAAGLGG
                     VKIVRVRRN"
     gene            complement(13714..17055)
                     /locus_tag="PLS34_00215"
     CDS             complement(13714..17055)
                     /locus_tag="PLS34_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP85925.1"
                     /translation="MILEFATAGALVLNAALLISVAHILALAISRRARRSRPAWSVVV
                     GLLLGLVGVAIMMFPFELQPGLVFDLRSVLLGASGLFFGAVPTLLAMAMTAAYRLLQG
                     GVGAFTGVVVILVSGLVGLAWRWWRRSAPVDIRWPELLAFGLVVNGAMLVVMPLTLPA
                     GLGRTVLAELGLPVLLIYPLATIVLGMLLAGRLKYQANVRALRDSEVRYRSLFHDNRA
                     VMLLVDPQSGTIVDANPAAAQFYGWTLDELPGMSFEKLNPGGRSLVVTRQSVDEARHK
                     RADGSMLDVETYSSPIEVADQHLLHLIIHDIGDRKAAEQRLADSEHQRATEHQAAMDK
                     QRRARLAALNLMEDSIQAREQAESVLDELQQTKSRLEFALRRSRAAGWELDLADLTVE
                     RSPEHDRIFGEPAPRAIWHYRDVLQFVTPEDQAMVEQSLLAALEARSDWECECRIRRA
                     DGEMRWVWLSGTHQFDAAGVATKLAGVVQDVTERKADEDLLRKLSQAVEQSAESIIIT
                     DSHGNIEYVNDTFVQSTGYRLEEVLGKNPRILKSGKTPAATYDTLWQTLLRGESWSGE
                     FCNRAKDGRLFYESAIITPLRRPDGRVTHYVAVKFDITENRELIAKLQHYRDHLEELV
                     EQRTRELAEARRNAEAANRAKSTFLANMSHEIRTPMNAIIGLTYLIRRDGVAPAQSER
                     LDRIDDAGRHLLTIIDDVLDLSKIEAGKLELELTSFELGSVLDNVASIVRESAQRKGL
                     EVHADHDHVPEWLYGDPTRLRQCLLNFAGNAVKFTESGRIDLRARLVAESGAELLVRF
                     EVEDTGIGIQPEDARRLFNAFEQGEASTSRRYGGTGLGLAITHRLTQMMGGEIGVEST
                     PGVGSRFWFTVPLGRGRGTAPAGDAAELPESAEALLRKRHAGARVLLAEDNDVNRQIA
                     VHLLEAAGLAVDTAVDGREAVEMAMQGDYRLILMDMQMPEMDGLEATEIIRSSTPCGH
                     LPILALTANAFAEDRLACRKAGMNDFITKPVRPALLYSTLLRWLNDSPSDGDAAPGKD
                     PLDEEQVSVSGGTTTRIGITFSVLDQLDSLLEREDPAAVSHYARHAALIRQLLGLASV
                     ELARQIMEADFAAARRTLADLRTRA"
     gene            complement(17052..18665)
                     /locus_tag="PLS34_00220"
     CDS             complement(17052..18665)
                     /locus_tag="PLS34_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRP85926.1"
                     /translation="MHPSETRPAPVSIAGGDDRAARRSLWLPLAVLGTLLLLANLVMA
                     NHVYQQKRADYWTIVWSVAESHSTQVANWLEERLRSAGLHATSFPQAELYTRWQVEGD
                     LHARDLLLLRLKQFADSGRFYAVNLLDGAGRPLAGDAHPTLQLPAESLDAAMMSAHRR
                     GAGFIGPFKDEHGSIHLDFLASLPVEGSESTPLVLLHTGASDYFPESLRSFPLPSTSG
                     EVLLFRVEGDAVVALHDLRHVEGSAFTRLVMSEAPDQLAVKIAGAGPADRARVEGVDY
                     RGEAVFGSGRAIPGTDWFLVAKMDISEVAGAALRGMLPIALLSLLCFSAVGATLVNRR
                     QRQHLRQARTIQKVQGERLQSLQLLKAVADSAEDAIFVKDPDGRYLLFNRAACQMTGR
                     SEDEVLGRDDYSVFPPEQAEMLAATHRRVLAENRAVMAREILHTAHGQRAVLARKGPL
                     HDDSGRLIGVYGISRDVTDLEHSSILLEEHGRQLEERNQELERFNEAMVGRELDMLRL
                     KQQVNELSVRLGLKPPFGRRVIELPEEES"
     gene            18845..19942
                     /locus_tag="PLS34_00225"
     CDS             18845..19942
                     /locus_tag="PLS34_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRP85927.1"
                     /translation="MESHPHTHPQATPLPPPDEMYRALVARDPGYDGIFVVGVRSTGI
                     FCRPVCPARKPRRENVDFFAGAREALAHGFRPCKRCRPLQPSGATPAAIAGLLAELDA
                     DPALRLRDGDLRVRGLEPATVRRWFKQHHGMTFHAYQRARRVGAALGTLAQGAGVTDT
                     AFGSGFDSTSGFQEAVRRITGRSAAASRNAPLVRLARVLTPLGPMVMGSTDTGVVLLE
                     FTDRRMLETQLQRLARKRKCAFVPGQTHAGRQLEQELAAYFEGRLTAFETPLEPAGTE
                     FQRAVWRVLKDIPYGQTRSYGQQATSLGRPSAVRAVARANGDNPIAIVVPCHRVIGAD
                     GTLTGYGGGLWRKRWLLHHEQSRLAGRVPAR"
     gene            19956..20678
                     /locus_tag="PLS34_00230"
     CDS             19956..20678
                     /locus_tag="PLS34_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRP85928.1"
                     /translation="MRHHLAAVALAPVLLVQGLRVRRVTPRLPEPPGPRSGIAGSGSP
                     LRLLIAGDSAAAGVGAETQSQGLSGVVVEALRRDYRVSWSLIARTGLRTAGVIERLEA
                     AAVEPFDVALLSVGVNDVTAFTPVGQWLAQQARLVALLKSRFGVRHVLLTCLPPMHAF
                     PALPQPLRWYLGARARTLNDHLVAFAAADPACEVVAPDLPPDPALMAADGFHPGPPAY
                     LEWGCSAARIIRRRVPSGPTWA"
     gene            20709..21176
                     /locus_tag="PLS34_00235"
     CDS             20709..21176
                     /locus_tag="PLS34_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRP85929.1"
                     /translation="MNIDLSISAAGVWALVGVVLILAEFAMTGVILVFFGVAALLVAL
                     LLWTGVDMSLNVQILVFGALAIGLLLVARDRVRSWFGGKSEQAGDGIEVLVPGTPVTA
                     LGDFVDGVGLVTHRGARWNAECDAPVAAGQRVWITGRRGLVLSVSTEQNAPGR"
     gene            21220..22167
                     /locus_tag="PLS34_00240"
     CDS             21220..22167
                     /locus_tag="PLS34_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HRP85930.1"
                     /translation="MQTVDLMTIISLVVLAIIVVALFKTAQIVPQRSAYIVERLGRYA
                     RTLDAGFHILIPFIDRVAYRQTLKEEAIDVPKQQCITRDNISVSVDGVLYLQVVDPHA
                     ASYGIADYHYATASLAQTTLRSVIGQIELDKTFEERAKINEEVVKALDEAANPWGVKV
                     LRYEIADIVVPTTITDALEQQMRAERERRAVVARSEGQRQEQINVSEGEKIQIINLSE
                     AQKQKQINEAEGKAREIQMLAEATGQGIERIATAINQPGGKEAVSLRIAEQYVREFGN
                     LARTNNTMILPAELSNIGAAVAGLAEVLNKAKPGSAGAG"
     gene            22345..22932
                     /locus_tag="PLS34_00245"
     CDS             22345..22932
                     /locus_tag="PLS34_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP85931.1"
                     /translation="MNAKLLALAGLLALPGAALATDLNYNYIEADYVTIDVDDIDEDF
                     DGWAVNGSFLITEQVFIFGGYSDISSDRIGGFKLSTEGVTLGLGYRHELTRQTDLNFA
                     GAFERAKAKAELGGFGSESVSENGYSLAVGVRHLVSPQLELGVGAVYVDIGDGDDTNA
                     TANALWHVTEAVALGVNLSLGSDATGYGVGVRLKF"
     gene            23069..24217
                     /locus_tag="PLS34_00250"
     CDS             23069..24217
                     /locus_tag="PLS34_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRP85932.1"
                     /translation="MRFIHTADWHLGRQFHNVSLIDDQRHVLRQLLDAAVGAEVDAVV
                     VAGDVFDRAVPSPEAVELLDEHLAELVLARGIPVIMIAGNHDSGRRLGFASGLLARSG
                     LHVWGRYEGRPPRAVLSDRHGVVHFLGLPYAEPAQVRELSGDPGLHSHAAAMHWLTRA
                     ALESVPAGERSVCVAHCFVAGGAESESERPLSVGGAAAVPAECFAGFSYTALGHLHRP
                     QAVGERLRYSGSLLKYSFSEILHRKSADLVELDATGGATVTTLPLQPLRDLRLLEGEL
                     ARLLEGPSPGENPEDFLLVRLSDEHELLDPMARLREIYPNVLHIERPALQRAGEDALR
                     AHRRQGSDVELFNAFYAQAAGVQLSEAQAGAFREVYDALLAQSREASR"
     gene            24214..26877
                     /locus_tag="PLS34_00255"
     CDS             24214..26877
                     /locus_tag="PLS34_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRP85933.1"
                     /translation="MRPLRLELTAFGPYAEPQVIDFGALGERRLFLVHGPTGAGKTTL
                     LDAICFALYGDTTGAERDGRGFRSQFAGPTLETTVTLDFQLGPHVYRVTRQPEQERAK
                     RVGQGTTVSPPSATLWDRTDIEDPAEEGRVLATRFGNVTAAIVDLLGFRSEQFRQVIV
                     LPQGRFRDVLLAGSREREDILRQLFDTGFYERIEEALKERSRELRVAAEQLRAERTAL
                     LRPEELEDEAALEACLQALAAEKRDVDPMLRSLQQRSSAAAEALERARGERARFEKAE
                     RAQRALAELQERSAEIDADRQRLQAARRAAGLVDLAAQLVTLRRSEVESRGLLEDLRA
                     RRSSASERLERAATAARAEQARSHERDALGQRAAELARLLPLAEELAKLLERESAETV
                     AQQAAAEGVEAAMEAAAAARADAAVLRGARESGSAALLAAELRPGEPCPVCGSTEHPR
                     PAAGGGEIPSGEALAAADEAIAAAEATLDEHRQASQRAAAVLSDLRARIDTLRRQLEG
                     VVEPTPARLRELPRLAEEAVRRGAALQEALDRAMAQEQSARDESARLEAAHQAAMVQD
                     GAARKALQAAEKEWSLRLDQAGFDSEAAWRSAALEEGEIERVQTQIDEFRQRLAAAEG
                     MAADSRAEVAGRAARPRCSEGRGDRGCGRVRRGRHAERAAGQPDRRAGTVARAATGHC
                     CGIYSDGAALRRVRQHRAGRCGRQLAPYFAASLRAREQARRRAGGCQPAPPRHDSWAL
                     PAQAQHRDRRPARRRWPGGTGRGRLHRQQPAGRNIVRRGELPGGAGTGARLVRSGTGL
                     LGRHQPGHDLHRRRLRQPGPGGARPGDRHPDGPAAVGPAGRSDFARAGTSGTYRRPSR
                     GGGGRCGQPRRLSFTLSRSARPRACSRPSFH"
     gene            26865..28094
                     /locus_tag="PLS34_00260"
     CDS             26865..28094
                     /locus_tag="PLS34_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP85934.1"
                     /translation="MVPLTALLSGAGLLLLGSGLLGTLLGVRGTLEGYDDRALGLIMS
                     AYFLGFFLGTFLAPAVIRRMGHIRAFSFFGALFASSVLLHAMWVDPLGWGLLRIGTGI
                     AVVGLYTVIESWLNATVDSTQRGRVFAVYMGINLVALALGQQLLRLAPAESFALFSLA
                     AILVCAAMLPVTATRLVQPVMQPATRIGARQLFGIAPAAAAGAMFSGLSVGAFWGLGP
                     VYAGRLELGIEGIATFMTMTIIGGAALQWPVGRLSDSGDRRRTLALACIGAAIAAGLA
                     VWYSGASAWPLYLLFFMYGGLSFAVYPVAVAHLLDHVPPEELLPACSTVLLLHGVGAA
                     FGPAIAGFAMAWLGAQAFPGYLMLVHLLLAVYVFLRLAARQRVAQGESHFHAMLRTSP
                     TAMEMLPEVEAAQDEAS"
     gene            complement(28099..29070)
                     /locus_tag="PLS34_00265"
     CDS             complement(28099..29070)
                     /locus_tag="PLS34_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HRP85935.1"
                     /translation="MAAALAPLQAEASPELHGLFATTDQNPFIQIYSLPSPAMHIAPG
                     TDRWTWGIELDVANGAIRDELPSGERLALDGETYRTSLLLRRQLSERVVAGLSIPFVA
                     HSGGLLDGFVRDWHDLFGLSNRRRDEFRDDALEYSYTNGAGQAFELTERSRGLGDVRL
                     TLDRSLATADPRRRLGLRGGLKLPTGSSDDLHGSGSTDVSLQLLVTDAATLSAWNTTL
                     AWMIGGLWLGDGDVLGGLRRDLVAVGSIGVSRPVWRRLDLRLQIDGHSSFYDTELEPL
                     GSAGVQLSFGGSIMLAQAGRLDLVLVENLFTDTTPDLVLHLAWRAAF"
     gene            29272..30066
                     /locus_tag="PLS34_00270"
     CDS             29272..30066
                     /locus_tag="PLS34_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRP85936.1"
                     /translation="MLCGVLSGWAGVAGAEQHAPPHAGASWAWSNATGVQGPVDPGED
                     SWPPRDRNPAGLRRDTAYLLSYQVVTIGILYALPESVSNWSDEQKESYSLADWWENVT
                     NPQWDTDDYYLNYILHPYWGAGYFVRARENGYDERASFWYSAAMSTAFEFGAEALFEQ
                     PSIQDLIVTPVAGAILGEYFMHLRAGILERRDPGEPLPLEDRLLLVLTDPIGAINRQV
                     QSWLGLSDAAAIQPYYAPQRVGAVRPYAVPDTPVDWVFGLRVDYRW"
     gene            30176..31486
                     /locus_tag="PLS34_00275"
     CDS             30176..31486
                     /locus_tag="PLS34_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRP85937.1"
                     /translation="MSKHRFETLQVHAGQAPAAGTYSRAVPIHQTTSYTFTDADHGAR
                     LFALQEFGNIYTRIMNPTTDVFEQRVAALEGGVAALATASGQSAQFLAITTLAEAGDN
                     IVSGASLYGGTYNQFKVQFPRIGIDVRFVATDDFDAWEAAIDDRTRALFVESIGNPGF
                     DVPHFRKLAELARKHELPLVVDNTFGCAGYLVRPIEYGADIVVQSATKWIGGHGTSIG
                     GVIVDAGKFNWFGKRFKTFHTPSPGYHGINFAAVFSPDGPFGNLAFIIRARVEGLRDW
                     GPALSPFNAFMFLQGLETLSLRVERHCDNALELARWLTTQPLVERVNYLGLESHPHHQ
                     RAREYLRHGFGSVLTFGIRGGLEAGKHFINSVELASHLANVGDAKTLVIHPASTTHQQ
                     LSADEQLSAGVLPDLVRVSVGIEHIDDIKEDFTQAFAKVASRGG"
     gene            31483..32547
                     /gene="metX"
                     /locus_tag="PLS34_00280"
     CDS             31483..32547
                     /gene="metX"
                     /locus_tag="PLS34_00280"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015128590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRP85938.1"
                     /translation="MSKSLLPAAAPVSKDTRLWRHDGPFCLESGVTLPAVQVAYRTWG
                     QLAPDGSNAVLVCHALTGSADADVWWEGLFGAGKALDPARDFIVCSNVLAGCYGSSGP
                     IQPHPEDGEPWGSRFPVVTIRDMVRLQSLLLDQLGVGELQLVLGPSLGGMQVLEWAAS
                     YPERVRAMAPIGVSGRHSAWCIAISESQRRAIYLDPDWRDGRYPLDRPPRYGLSVARM
                     IAMCSYRSWDNFEARFGRKTQEWDGQFAVTSYLNYQGEKLYKRFDANTYVRLTQAMDS
                     HDLARGRGDYAEVLGGLRMPALVVSVDSDVLYPPHEQAELARLLPAARYEMLHTPAGH
                     DGFLIETDVLSDMLLSFRSS"
     gene            32771..33028
                     /locus_tag="PLS34_00285"
     CDS             32771..33028
                     /locus_tag="PLS34_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRP85939.1"
                     /translation="MCLAVPVRVVAVPSPEWAIVDLGGVESRVSIVFVDDVTPGDYLI
                     VHVGTAIARLDVEQAEESLALFRQIAETVGIETDALHTRLP"
     gene            33003..34133
                     /gene="hypD"
                     /locus_tag="PLS34_00290"
     CDS             33003..34133
                     /gene="hypD"
                     /locus_tag="PLS34_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRP85940.1"
                     /translation="MRYIRGFRDGRAARGLARLIAAETRSDREYHLMEFCGGHTHAVF
                     RHGLPDLLPDNVRLVHGPGCPVCVLAVPRLDLAIELAFDERVILASYGDMLRVPASRK
                     KSLLKARAAGRDVRIVQSALDALDIAQRHPEREVVFFGIGFETTTPPTALVIREAERR
                     GLENFSVYCNHVLTPSAILSILDSPEVRELGQARVDGFLGPSHVSTVIGARPYEYFAE
                     EYQRPVVIAGFEPLDVLAAVRMLIRQLNEGRAEVENQFTRGVTRDGNLKAQAMVAEVF
                     ELRPEFEWRGLGKVQYSGLRIKARYARFDAERRFDLVEKPAVENKACQCPAIIRGLKK
                     PTDCPIFGTVCTPRNPIGACMVSSEGACAAWYQYRNGLAETA"
     gene            34144..>34915
                     /locus_tag="PLS34_00295"
     CDS             34144..>34915
                     /locus_tag="PLS34_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRP85941.1"
                     /translation="MRILILTHSFNSLAQRIHAELALLGHDLTVELDISDEVTESAVA
                     LFRPDLVVAPFLKRAIPPSVWRRVPCFIVHPGIRGDRGPSALDWAILEGRETWGVTVL
                     QAEAEMDGGPVWAWREFPMRLAAKGSIYRFEVTEAAVAAVLEAVDKLAEGDFRAEAPD
                     PADRAIAGGWRDSMRQSDRRIDWQQDDTIKVLRKIHSADGHPGVRDNIAGRPVRLYAA
                     SAEYRLAGAPGELLAQRDGAVCRATVDGAVWIGQVRAEA"
BASE COUNT         5468 a        11546 c        12018 g         5883 t
ORIGIN      
        1 ttcgaacccg cgttcaatac agcccgggtt cgagccgagg ccggcgccag ccggccgaga
       61 cgccggcccg gacgggccga ccatcccgcc ctctccgcca aatcaagctc agtcgactat
      121 tggattcgaa cccgcgttca gcgaggtcgc ttcgcgagcc gccaggaaac cagcatcgcg
      181 accacggcgc cggcaaatgc cagggcagtg tccttctggg catcccactc gtcgccctgg
      241 gtgccaaggt aggcagcgcc gagctccggg cttacgatca tcgcgacaat gccctcgagt
      301 gcttcgtaaa atccgctgaa gccgagaatg gacagggtcg ccacggggta agaccagccg
      361 gacctcgtgc cggcggcgcg gaccagtaac tcgcggatcg gcggcgccac gagcagcccg
      421 aagcagaagt gcacgaagcg atcgtagtgg ttgcgtgaca agtccagtgc atcctgcaac
      481 cagaatcccg ggggtacttc actgtaggtg tagtgggcgc caaccaggtg caatgtgagg
      541 aacagcccca gcaggccgta ggagaaattc gagaaagtga aacgccgggc ggtgagcagc
      601 agtgtcgctg caaatacgaa aaccaggagg ttctccagca gccagtcacg gcgactgtag
      661 ggcgctatgg cggcgatgat ccagatggcc accagccaga gcagcagggc cctgatgaaa
      721 cgacggccgc tgaaggtccc gttcatccgc cagcctccct tgccgtccgc ccggcattcg
      781 ctgatgaaat tagcatcttt ccgcgaaatt ttgtcacttg ccgcgtgccg ctttttgtat
      841 acttcatttt ttcacgggag gttcgccatg cagattcctg gccggctcgc cccgctgttg
      901 atcggcctca tcgcggcggt cggcgccacg ccggcaggcg ccagcgattc caggccgggt
      961 ggcagcctgt tcatctataa ctggtccgac tacatcgccg aggacaccat cgcccagttc
     1021 gaggcggaga ccggcatcaa ggtcacctac gacgtcttcg acagcaatga ggtgctcgag
     1081 gcgaagctgc tggccggcag caccgggtac gacctggtgg tgccttcgct ggccttcctc
     1141 gggcgccaga tcatcgccgg ggttttccag cccatcgacc gcagcaagct gtcgaactac
     1201 gacaagctcg atccgcagct gctggcgcga attgccgagg tagacccgga caacaagcat
     1261 gccgtgcctt acctgtgggg cacgacgggc atcggttaca acgtcgaaaa gattgcagcg
     1321 atcttcggcg aggacttcga ggtcaattcc tgggacctgg tgttcgaccc ggcctatgcc
     1381 gagaagatct cggcctgcgg cttttccatg ctagatgcgg cctcggagat gctgccgatc
     1441 gcgctgcact acatcggcga ggatccgaac agcttcgatc cggcggtgat tgcgaagtcc
     1501 tatgacgtgc ttgcggccgt gcggccgtac gtgacgtact ttcattcctc ccagtacatg
     1561 aatgaccttg ccaacggcga catctgcgtc gcggtgggct ggtccggcga cgtgttccag
     1621 gcgatagacc gggcggacga ggccgagcgc ggcgtcgaaa tcgactacgt gatccccgcg
     1681 gaaggcgcgc cggtctggtt cgacatgctg gccatcccgg cggattcacg caacgtggag
     1741 aacgcgcatc gcttcatcga tttcctgctc cggcccgagg tcatggccgc aatcaccgag
     1801 tacgtcgcct acgccaaccc ggtgcccgcc tcgcgggagt tgatggacga ggagttgcgc
     1861 gacgacccgg acatcttccc gccgccggag gtcatggcaa ggctctacgg gctgaaggtc
     1921 ctgccacgcg acgtggaccg cgtgtacacg cgcacctgga cacgagtccg gaccgggcgg
     1981 tgaattgacc gcgaggcgcg gcgcggcccg ctcgcccggg caaagcgaat gagcaacggc
     2041 cagaagcccg ccggggccgc gcgcgctgcc gtttcccccg ctcgggccga agcgctcggc
     2101 cgccaggaat acctgaagat cgaggacgtc tcgaaggact tcgacggctt cgtcgcggtg
     2161 gaccgcgtca cgctctcgat acgccagcgt gagatcttcg cgctgctggg cgcctccggc
     2221 tgcggcaagt ccaccctgct gcgtctgctg gcgggcttcg aggcgccgtc ctccgggcac
     2281 atccggctgg acgggcagct gctcgacggg ctgccgccgt accggcggcc ggtgaacatg
     2341 atgttccagt cctacgcgct gtttccgcac atgaccgtgg ccgacaacat cgccttcggc
     2401 ctgaagcaag acgggctgtc gcgcgaagag cgcaagcacc gggttgatga gatgctggcg
     2461 ctggtgaaga tggagtccat ggcgcgccgc aagccgcacc agttgtccgg cggccagcaa
     2521 cagcgcgtcg cactggcgcg ctcgctggcc aagcggccca agttgctgct gcttgacgag
     2581 ccgatgggcg ccctcgacaa gaaactgcgc tcggagatgc agctcgaagt cgtcaacatc
     2641 atcgagcagc tcggcgtcac ctgcgtcatg gtcacgcatg accaggaaga ggccatgacc
     2701 atggcccacc gcatcgccat catggaccag ggcggaatcg tgcaggtcgg cacgccggac
     2761 gaaatctacg agcagccggc cacgcgcttc gcggcggcct tcgtcggttc ggtcaatctc
     2821 attgaaggca agatcgaggt agacgcaccg gaccacatca ccatccgcgc cacggggctg
     2881 ccggagccga tccgggtggg ccacggacat accggctacg agggcatgga cgtgattttc
     2941 gccctgcgtc cggagaagct ggtcatcagc aaggagcccc cggaccagtc caacaaccgc
     3001 taccagggca agatcgagga aatcgcctac ctcggcagcc actcggtcat gcacatccgc
     3061 ctgccgagcg ggctcaagtt gctggcccat gtgcccaact cgcggcgctg ggcctccgag
     3121 gactttacct gggaggacca ggtgtgggcg agctgggacg acatggcggg cgtggtgctg
     3181 tcgtcgtgat caagcggtcc ggcttgcgat tgccggggac gcgctggctg gtcatcgccg
     3241 tgccgttcct gtggatgctg gtgttctttg cggtgccttt cgccatcgtg ctgaagatct
     3301 cctttgccga gggcctcatc gcgatgccgc cctacagcga cctcgtaacc cgcaccgaca
     3361 cgggcatcac catcagcctg aacctcgaaa actacctcta catggtgcgc gacagtctct
     3421 atgtctcggc ctatctcggc tcgctgaaga tcgccgtcgt ttccaccctg ctgtgcctgc
     3481 tgatcggcta cccgatggcc tacgcaattt cgcggctcga tccggccgcg cgcaatctcg
     3541 cgctgctgct ggtgatcctg ccgtcctgga ccgccttcct gatccgggtg tatgcatgga
     3601 tcggcattct caagaacaac ggtgtgatca acaatttcct ggtatggatc ggcgcgatcg
     3661 gcgaaccgct gcagctgctg cacacgccgc tcgcggtgta catcggcgtg gtctacatgt
     3721 acctgccgtt catgatcctg ccgctctacg ccagcctcgt gcgcctggac ccgcgccacg
     3781 aggaagcggc ccaggacctc ggggcgcggc cctggcgggt gttcgtaagt gtcacgctgc
     3841 cgcgcactct cgccggtatc atcgccggct cgatgctggt gatgattccg gtgatcggcg
     3901 aattcgtcat ccccgaactg ctcggtggcc ccgacacact catgatcggc aaagtgctgt
     3961 gggaagagtt cttcaacaat cgtgactggc cggtggcctc ggcggtggcg atcgtcatgc
     4021 tgctgctgct gatcgtgccg atcacgattt ttcaccgctt ccagtcgcgc gagatggaga
     4081 aacgactggc atgaggcgcg tcggccgcaa ttcaccgttc acgctggccg tgctgggact
     4141 cggcttcgtg ttcctgtacg cgccgatcct cagcctggtg atctattcct tcaacgaatc
     4201 gcggctggtg acggtctgga gcggcttttc gctgaagtgg tacggcgagc tgttcgccga
     4261 ccggcagctg atggacgccg tgtggatcag cctgcgggtg gctttccaga ccgcctggtc
     4321 cgcggtggcc atcggcacca tggcggcaat gctgctggtc aggttccgtc gcttccgagg
     4381 caagaccctg ttcggcgcca tgatcacggc gccgctggtc atgcccgaag tcatcaccgg
     4441 cctgtcgctg ctgttgctgt tcgtccatat cggcgagctg ctcggcacgc ccatcatgcg
     4501 tggcatgggc accatctgga ttgcccacac gaccttcgcc acggccttcg tcaccgtggt
     4561 cgtggcctcg cggctgaccg agatggatcg ctccctggag gaggcggcga tggatctcgg
     4621 cgccaaccgc ctcaaggtgt tcttcgtcat cacgctgccg ctgatcgtgc cggcgctggt
     4681 ggccggctgg ctgctggcct tcacgctgtc gctggacgac ctggtgatcg ccagcttcgt
     4741 gtccgggccg gcatcgacca cgctgccgat gaaggtgttc tcgtcggtgc gcatgggcgt
     4801 gagcccgaag atcaatgcgc tcgccacgct gtggatcgcg gcggtgtcgc tggcggcgat
     4861 cgtcgcctgg cgcctgatgg cgcgagggga acgcaggcgc cggcgggaat tgcgggccgc
     4921 agaatgaact ggtcaacctc gggcgcgggg cccgtcaaca caagggaaac gaagatgtgg
     4981 aagaaatttg cgccggccat gctggcggga ttcgcactct gcctgctgtt cgtgaatgcg
     5041 catgcagacg aggacgaggg ctggtggttc ggcggcagcc tggcggcgac gagcgactac
     5101 gtatttcgcg gcgtgtcgca gaccgacgag ggaccggcct tgcagggcag cctcgatgtc
     5161 ggccactcca gcggcctgta cgccgggatc tgggcctcga acgtggactt cgactcgccg
     5221 gacggcatcg atctcgagct gaacttctac ctcggctggg ccttcgagtt cgacaacggc
     5281 accgggctgg acctgcagct ggtgcgatac cagtacccca gcgcgaaccc gggttacggc
     5341 atcaactaca atgagttgat catcagctac agcttcctcg agtcctacac ggcgaccgtc
     5401 gcgtactccg acgatttcct gcgcagtggc gagagcgcgc tgtactacca tctcggcgct
     5461 gactttccgc tgggcgtcgc cgaactcaat ctcacgctgg gcgctggcct caacgacatc
     5521 agcaatgtga ccggcagcga ctactgggac taccaggtcg gcatcaaccg cgacttcggc
     5581 atcgtcaacg cggacctctc gtatttcgac acctcgggct tcgacgccga cgtgcaggac
     5641 tggctcgggc cgccgaactg ggcggacggc cgcatcgtat tgacgctgag cgtggagttc
     5701 tgacaccacg aggtccaggc attgtcatgc tgcagagcta cgtaaagaag attctgtcct
     5761 cgcgagtcta cgacgtcgcg gtcgagacgc ccttgcaggc ggcgccgggc ctgtcgcggc
     5821 gcagcggctg cacggtgatg ctcaagcgcg aggacctgca gccggtcttc tccttcaaga
     5881 ttcgcggtgc ctacaacaag atcgcgcagc tgcccgaggc cgagcgcgcc cggggcgtaa
     5941 tcaccgcttc ggccgggaat cacgcccagg gcgtggccct ggcggcccgc gagctcggac
     6001 tgaagtccac catcgtgatg ggccgcaata caccgagtat caaggtcgac gccgtgcggg
     6061 cgctcggcgc gcgggtcgtg ctgcacgggg acacctacga cgatgcggcc gcgcacgcgg
     6121 ccgagctggt ggcgcgccac ggcctggtgt atgtcccgcc gtacgatgac ctcgacgtca
     6181 tcgccgggca gggcacggtg ggcatggaga tcctcaagca acacgccggc gcgccgacgg
     6241 cgattctcgt gccggtgggc gggggtggcc tggtcgccgg gatcgccgcc tacgtgaaat
     6301 acctgagtcc cgggacccgc atgatcggcg tggaggcaga aggttctgcc tgcctggcgg
     6361 ccgcgctggc ggccgggcgg cgcgtgacgc tgccctatga aagcctggac ctgttcgccg
     6421 acggcgtgtc ggtcgcccag gtcggccgcg agcccttcaa ggtagcgcgg cgttgcgtcg
     6481 acgaggtcat cgtcgccaac accgacgaaa tctgcgccgc catcaaggac ctgttcgagg
     6541 acacccgctc gatcgcagag cctgccggtg cgctggccgt cgccggcctg aagaaatggg
     6601 cggcgcgtga agccgaggcg ggcaggctgg cgccggcggc gagtgtcgtc gccatcgtca
     6661 gcggcgccaa cgtcaacttt gatcgtcttc gtcacatcgc ggagcgcgcc gaggtcggcg
     6721 agcatcgcga ggccattctg gccgtcacca ttcccgaaaa gccgggcagc tttcgccgct
     6781 tttgtgccgt catcggcaag cgctcggtca gcgagttcaa ctatcggtat gcggatccga
     6841 gccaggccca tgtctatgtc ggcctgaaga tcggcgcccc ggaggaccgg gaaaccgtca
     6901 agaccctgct ggacgccgcg ggctacggcg tggtggacat gaccgaaaac gaggccgcca
     6961 agctgcacgt gcgccacatg gttggcgggc gtcccgcagg agacaagccg gaactgctgt
     7021 tccgctttga gttcccggag cggccggggg cgctgctgaa cttcctttcc gggctgggcg
     7081 cggaatggaa catcacgctg ttccactacc gcaatcacgg cgcggcctgg gggcgcgtgc
     7141 tggtcggttt cgaggccgag cgtcgcgacc gcacgcgcct ggtcggcgac ctgaagcgca
     7201 tcggctaccg attctgggaa gagagcgaaa acccggcgta ccggctgttc ctgcgctgag
     7261 ttcggtctgg ctggaggctg attcccgcat tccgcctcag agcccgcgtg ctgatggacg
     7321 gggattggaa cttgcctcgc ccggacgcgg ctggcatctt cgcggccatg acgagcaatt
     7381 cggagtaccg cggcagcacg agcacatccg ggcaggacga tgctgctcgt cgccgggcct
     7441 tgcggccggg ccggctgccg accgcggtgc aggtgccggt gctcctgaca ggcctggcga
     7501 ccggactcct ggtgctgatg atcagttccg tgctcgatgc gcggctggtc gttgccggtg
     7561 cctggttcat ggtcgcctac tgcggcctgg tgctgttgtc gctgcacccg ccaccgtttt
     7621 tttccgccca gctggacagg ttcctcgatg agtgggtcga agacctgggc gtcggctact
     7681 acgctgtggc cgcgctggcg ttcttcatac cccgccagat tcagacgacc ctgtcgggcg
     7741 acttttcctc cttcgtgatc gagtggatcc tgaaattcag catggagact tttttcaatg
     7801 tcttccatgc catgacgtgg ccgttcctgc tcatcaaccg acacggcatg tggccggcgc
     7861 tgggctgggt cgtcgcgacc tacggcgtgt tctggatcgg tacgcgggcg ttctcgacgc
     7921 ccgcctggat gctggaaaaa gacgaccggg acgaggccga ggcgaactga cgccttcagt
     7981 cgcgcgctgc tggccgcgcg aagctgtcga atttcctgtc cgggatgctg gcgcgcatgg
     8041 tcgactcgct aacgcaaccc ggccacctcg agtccctgct cgaataccac gtccaccggg
     8101 atgccggccg cgttgatccg tgcgaggtcc tcggccagct cgggccggat gacgccgtgt
     8161 gaggcgagca gggcgccggc ccctgcgtgg tctccgtcgc cctgcagcac caggatgcgg
     8221 ccggccagta cgcgcaccgc ctcctgcatg cgctccagat cgacgcggta gcggccgtcg
     8281 gcgccgcgtg cgaacgcgcc gcgctccgag aagtcgttga actggatcat gttggcacgg
     8341 ccgtgggcgc tcgaggcgcc gaagcgcacc gagcggaaca ggccggcgag gaaggtgacg
     8401 taatggtcga gcagctcgcc ttcgtgcaac tcgccgcgct cgaacagctc ggtgatcatg
     8461 tagagaccca gcacgtcggc tttggcttct tccagcgccg agccgtgttc gcgcatcgcc
     8521 tcgcgtaccg tgccgcgacc gtcgagcgta ttcttgatgc ccagcccgtg ggccacttcg
     8581 tggaacatca cgttggagaa gaacgcgttg aaagtgatgt ggcgctgcag gtcctccgcg
     8641 atcaggtcgc gcgcgatcgg caccaggatc tggtcgaatt ttgcccgcat cgagttgcgt
     8701 atctgtagcc gccgggtgcc tttctcgagc tggacctgct catcgttcgg caggttgatg
     8761 gcgatggtct tggacccggc gttggcgtcg ccggcgtagt agatggcgtc gtaggcattc
     8821 aggtcggcgt cgctgccggg cgtctcggcc ttgtaggcgg cggggacggg caggccgcgc
     8881 tgcaattccg gcaggaactc cgcaaagcgc gccagccgct ctgaccaggt gaggtccttg
     8941 acgagcacga aggcctcgaa cgaggcacgg tacccgaaca tgcgatcctc gtaggtctcg
     9001 atcgggccga tgacgagctc gatgcggttg tccttcatgt ccatccacgc catgtccgag
     9061 ggctggtact cgtcagtgac cagcgcagcg gcgcgcagct ccaggtaccg gcggagtgcg
     9121 tcgttgccga ccagcgtcgc ggcgtcccgc aacaggcccg cggcctgctg gagttcctgg
     9181 cgatgcatga cggagtaggg caccaggacg agccgcccgg cgtcgtcgcg ccgcacatag
     9241 gaatagaggc ccgtcttgcc aggctggtcc catgcatcga actcctcccg ggacatgtcc
     9301 ggcggataga agtttgcgcc cagcggcttg tcgccatagc ccggcacgaa cggccggttg
     9361 ccgtccagcc ggttccaggg cccgtaattg atcctggcga agtccagcag ggccggatcc
     9421 tcgatgctcg ccagcagcga gtcccggtcg ccgtatgcgc cgtaccagaa cagcccgtcc
     9481 atgatctcgg ccgcacggat catcaactcg agcgcgcgcc gctcgtcgtc gctgaacgag
     9541 gtgagatcgg cgcgcagcgg caccgtggcg tagcgcgtca gttcgactgg aggcgttgcg
     9601 tccgcggtgg cctcgatggc cggggtcccg ttccccgggc cggattccga gcacgccgcc
     9661 acacaaaaca ccaacggcat gaaggccaac gggaacttga gtcgcatggg ctgtactccg
     9721 gcgtgaaagg taccgattat aagtgagcgg cggccgcccg gacccccgcc gttcatcgtg
     9781 cacggcgtgc tgcgcagcat gctgcgtcga gcacaccgca ctcatatgct agaatccgcg
     9841 gcgccagcag catgctagcg ctggaggagt cgcaaatgcg aatccggcga gccttgaagt
     9901 ggttcgggat cggcctgctc ggaatcgcgg gcctcgcggc ctcggtcgtg cttggcatct
     9961 acgttgccat gggacgcgac cttggcagga gctttgcagt gtcgggcgaa gaggtgttcg
    10021 tccccaccga cgcggccagt atcgaggaag gacgccgact cgcgcagttg cgcggctgca
    10081 cggtgggctg ccacggcctg gcctcggggg gcgcggtcat ggtcgagctg ttcgacggca
    10141 gccgggtcgt ggcccccgac ctggggcgga tcgccgccgg ctacagcacc gtcgaactgg
    10201 agcgcgccat ccgccacggc gtgaggccgg acggcaccag cgtcatccgt atcatgccgg
    10261 tcgaaatgtt ctcaggactg agcgaccgcg acctcggcct gatcgtcgcg tacctccgca
    10321 gcctgccgcc gggcgcgcag gaattgccgg acagtcgcta cggccccctc gcgcgcatca
    10381 tggggttcat gttcgcgcgt gaattcggat cggtcctggc tgccgaggtg accgatcatg
    10441 tccacccgcc accgcccgac atggaggatc cggccgcagc cggccgctac ctggcgcaga
    10501 ccgtctgcac ggagtgccat ggtgacgacc tgcgcggcgc ccccgacggt tcaactccaa
    10561 gcctggccgc ggccgcagcc tattcgcgcg atcagttcga aacgctcatg cgcacgggcg
    10621 agccgctggg cgatcgggag ctcggcctga tgggcggcgt tgcgaggtca cgcttcgttg
    10681 gatttaccga cgaagaaatc accatgctgc acgactacct gcggaatccg gcgacctggg
    10741 cggagtgagc ggcaccggtc cggctcatgg ctcccagccg tcgaggcagc ccgcggcgcg
    10801 caggcgcgcc tccagcggct ggagcgccgc acggaagtgg cgccacttgc ctaccgacga
    10861 gcggtagatc gcctgccgga cctggcccgc gctggcggta gtggacgggg cgctgttgcg
    10921 atggaactcc aggcacgcag gctcccaggg tggcccgcag aatgccagca gccggcgtgc
    10981 ttcgtcctcc agccggtcca ccacgtcctc gtagcgcacc acgtgcaggt cgtcacccag
    11041 tactgcgatc cagtgacgca tgagccgctc gtgtgcaatg aagtacccag ccagttcgtc
    11101 caggtcgtag gagtacgcga agctgtccgt gaaaatctgc ttgaacatgg agtagcaggc
    11161 gtccatgggg tggcgtcgca ccagcaccag tcgcgcctcg ggtagcgctc gatggatgag
    11221 cccgagatag atcgagttct gcggcaactt gtcgataaac cacggcgtgg tcccggccag
    11281 cggctgcgcc agttcgaggt agtcccggcc gagcgcggcg aagtccaggc cggtcgaggc
    11341 cggcaccatc gcggcccggc tgtgcccctc gagcccgggc aggcgttcca tggcgcggtt
    11401 caggcagcgt acgaaatccg gcagctcgcc ggcgctgtgc accgcgctgt gcgcgccgag
    11461 aatccgctcc accaacgtgg tgccggtacg cggcagtccg gtgatgaaga ccggcacgcg
    11521 ctcgcttgcc ggggcggcgg cgtcgcgccg cctgacctcg gccgcgttcc aggtggtgcc
    11581 gatcgcctcc aggaagcgga cttcgtcgct caggtcgaac tgcagcgtgc cgcggtacag
    11641 gcgggcccca cgtagcagga tcgggaagct ctggtcgtag cgtcccaggt cctccagctc
    11701 cttggcgagc gcaaactgga tcctggcgtg cgcgtccggg gctgtcggcg ggctgcccag
    11761 cgcggccttc agctcttcga tatggttgtc gtcgtgcgtc tggcgacgca gtccggagcg
    11821 aaagaacagc gcgtccggat aaccgggctg gagcgcgagg cttcgttcgc aggcagcgcc
    11881 ggcggcgccc aggttgccga gcgcaagctc gaggtttgcc agtcccaggt ggagtgatgc
    11941 agcgccgggc tggagcgaga tggcgcactg cagggcaagg cgggccgcgt cgaactgctg
    12001 caggctgtgc agcgcgtggc cgagcgcggc aaagtaccgc gcgtcgtcca ctttcacgtc
    12061 cagcaacgct gcggcgagcg cgatggcgtg gctacggcgg ccgtcctgga gcagctgcca
    12121 gagctgctgg agtcgggccg ctgcctgtcg cgagtccggc tttgctgggt cggtcatccg
    12181 gcaaggttac caatttcctc ggccggcgaa tgccgtgatg cggtgcacag cacgcgttca
    12241 agcaatccgg caggatagcc gccatacaga ccaggggccg tgtccccgag gacgggattg
    12301 ccatgaagct tggagtcgag aacctgcacg cgagtgcgcc ggccaggtcg acccgtgcgg
    12361 cccgtccgct gcggctggcc gtcgcggcgc tggtggccgg cgcgctgggc gcctgtggcg
    12421 gcaacggtga tgaaaccggc ccgacgatcg tggacccggt gatcctcgat ccggtggccg
    12481 atgcacagga tgagtccaat gaccgcattg agatcaccaa cacccagtcg gcgctcgata
    12541 tccgggtcat ctaccccgaa acccccatcc cggtggatgc cgacggcgcc ggctacggct
    12601 ccgaggcgcc ggtggcgatg gaacgcatcg acttcgacca gttggcctcg gtcacgctga
    12661 cgcttgcctc tgagatcagt ccaccggtgg tcgacaacca ggtagtgcag gccacggcga
    12721 ttgcacagag catcggcaac aacgccgtcg tcagctacaa catgcgcggc gagccacgcc
    12781 tcggggcgat cgactgggtg atgcgcctca acagcgcacg gcctgcgctc ggctcgagtg
    12841 ccactttcaa cgacgccgac gtcagcgctg tttctaccga cgggcgcaat gtctacatgg
    12901 ccatggcctc cggcgcgccg gagtttccgt ttccggcggt cgtggagcgc ctgcgcgtcg
    12961 agggcaatcg cctcacgctg gcgggcaatg agcgcgcgcc gctgaccagc ttcgtagcca
    13021 ccagcgtggt ggccgtcggc gatacggtgt acgcgacgtc cggcaacacg ggcggtgtgt
    13081 tcgctctggc cgaggatgac ctgggtcggc tcggcgagtt tccgctgcac gatgcccgct
    13141 gggtcgcgtc cgacccggag ggcgggcgca tcgcggtcgt gcaggggacc cccggccagg
    13201 tctcggtgtt cgccgggggc gaatttgccg gcggcggcat gacgctgttg aacacctttc
    13261 ccttttccgg cgccgacgtc ccggagtcca agtccacggt ggaggtcgcc ggcaacaagg
    13321 ccttcatcgc cgccggcccg gaaggggtcc aggtgatgtg cctcgacgac ggcgagatcg
    13381 tcggccacgt gccgcggccg gacccggcgc agctcggcct ggattcttca gtagtggtca
    13441 gcaacgcggt gaccgtggac ggcgacctga tgttcatctc caacggcgag gcgggcgtct
    13501 atgtcgcgcg cgccgcccag cccttcgcgt catccgactg ttcgccccag gacatcaccg
    13561 tgatcggcaa gctgcgcttc gacgagctgc agtctgccaa ccacgtgtcc tttcgcggcg
    13621 actatttgtt cgtggctgcg ggcctcggtg gcgtcaagat cgtgcgggtg cgccgcaact
    13681 gagcccagcc cgcccgacac atggcggcag ggctcaggcg cgggtgcgca ggtcggccag
    13741 ggtccggcga gcggctgcga agtcggcttc catgatctgg cgcgcgagct cgaccgacgc
    13801 aaggccgagc agctgacgta tcagcgcggc gtgccgggcg taatgcgata ccgcggccgg
    13861 atcctcgcgc tcgagcaggc tgtcgagctg gtccagcacc gagaaggtga tgccgatgcg
    13921 cgtggtggtg ccgccgctga cgctgacctg ctcctcgtcc agcgggtcct tcccgggcgc
    13981 cgcgtcgccg tccgacggtg aatcattgag ccacctgagg agcgtcgagt agagcagggc
    14041 gggccgcacc ggcttggtga tgaagtcgtt cataccggcc ttgcggcagg ccagccggtc
    14101 ttcggcaaag gcattcgccg tcagggcgag aatcggcagg tgcccgcaag gcgttgagct
    14161 gcggatgatc tcggtggcct cgaggccgtc catctcgggc atctgcatgt ccatcaggat
    14221 gagccggtag tcgccctgca tggccatctc gacggcctcg cgcccgtcca cggcggtgtc
    14281 cactgccagc cctgcagcct ccaggagatg gactgcgatc tgccggttca cgtcgttgtc
    14341 ttcggccaac aggacccggg ctcccgcgtg gcgctttcgc agtagcgcct ccgcactctc
    14401 cggcagttcg gccgcgtccc cggccggcgc cgtgccgcgg ccgcggccga gggggaccgt
    14461 gaaccagaag cggctgccga ccccgggcgt gctttccacg ccgatctcgc cccccatcat
    14521 ctgggtcagg cgatgggtga tggcgagccc gagtccggtg ccgccgtagc ggcgcgacgt
    14581 cgaggcttcc ccctgctcga aggcgttgaa cagtcgcctg gcgtcctccg gctggatgcc
    14641 gatacccgtg tcctccactt cgaacctgac gagcaactcc gcgccggact ctgcgaccag
    14701 cctggcacga agatcgatgc ggccggactc ggtgaacttg accgcgttgc cggcgaagtt
    14761 gagcaggcac tggcgcagcc gcgtcgggtc cccgtacagc cactcgggga cgtggtcgtg
    14821 gtcggcatgc acttcgagcc ccttgcgctg ggcagactcg cgcacgatcg aagcgacgtt
    14881 gtccagcacg ctgcccagct cgaaactggt cagctccagc tccagcttgc cggcctcgat
    14941 tttcgacagg tcgaggacgt cgtcgatgat ggtgagcagg tgacgcccgg cgtcgtcgat
    15001 cctgtccagg cgctcgctct gtgccggcgc cacgccgtcc ctgcggatca ggtaggtcaa
    15061 gccgatgatg gcgttcatcg gcgtgcggat ctcgtggctc atgttggcga ggaaggtgct
    15121 cttggcgcgg ttggcggctt cggcgttgcg tcgggcctcg gccagctcgc gggtgcgctg
    15181 ttcgaccagc tcctcgagat gatcgcggta gtgctgcagc ttggcgatga gctcgcgatt
    15241 ttcagtaatg tcgaatttca ccgccacgta gtgggttacc ctgccgtccg gccggcgcag
    15301 cggcgtgatg atggcggact cgtagaacag gcgcccgtcc ttggcgcgat tgcagaactc
    15361 gcccgaccag ctttcgccgc gcagaagcgt ctgccacaag gtgtcatagg tcgcagccgg
    15421 cgtcttgccc gacttcagga tgcgggggtt tttcccgaga acttcctcga ggcggtaacc
    15481 ggttgactgc acgaaggtgt cgttgacgta ctcgatgttg ccgtgactgt ccgtgatgat
    15541 gatgctctcc gcgctctgct cgacggcctg cgaaagcttg cgcagcaagt cctcgtccgc
    15601 cttgcgttcg gtgacgtcct gcacgacccc ggcgagcttg gtcgccaccc ccgccgcgtc
    15661 gaactggtgg gtgccggaca gccagaccca gcgcatctcg ccgtccgcgc ggcggatgcg
    15721 acattcacac tcccagtccg agcgtgcctc cagggcggca agcaggctct gctcgaccat
    15781 ggcctgatcc tcgggagtca cgaactggag cacgtcccgg tagtgccata tcgcgcgggg
    15841 agccggttcg ccgaagatgc ggtcatgctc cggcgagcgc tcgacggtga ggtccgccag
    15901 gtcgagttcc cagcctgcgg cgcgactgcg gcgcagggcg aactccagcc tgctcttcgt
    15961 ttgctggagc tcgtcgagca cgctttccgc ctgctcgcgg gcctggatcg agtcttccat
    16021 caggttgagg gcggccagcc gtgcgcggcg ctgcttgtcc atcgccgcct ggtgctcggt
    16081 ggctctttgg tgctcgctgt cggcgagacg ctgctcggct gccttgcgat cgccgatgtc
    16141 gtgaatgatc aggtgcagca ggtgctggtc cgcaacctcg atggggctgc tgtaggtctc
    16201 gacgtcgagc atcgagccgt cagcccgctt gtgacgtgct tcatccacgc tctgtcgcgt
    16261 caccaccagc gagcgcccgc cgggattgag tttctcgaag gacatgccag gcagttcatc
    16321 gagtgtccag ccgtagaact gggccgccgc agggttggcg tcgacgatgg tgccggactg
    16381 cggatcgacg agcagcatga cggcccggtt gtcatgaaac aggctgcgat agcggacctc
    16441 gctgtcccgc aaggcgcgca cgttggcctg gtacttcagc cggccggcga gcagcatgcc
    16501 gagcacgatc gtcgccagtg gatagatgag cagcacgggc aggccgagtt cggccaggac
    16561 ggtcctcccc agtccggccg gcagggtcag cggcatcacc acgagcatcg cgccgttcac
    16621 caccagcccg aaggccagca gctcgggcca gcggatgtcg acgggagcgc tccggcgcca
    16681 ccagcgccag gccaggccga cgagtccgga caccaggatg acgacgaccc cggtgaacgc
    16741 gcccaccccg ccctgcagca ggcgataggc cgcggtcatc gccatcgcca ggagtgtcgg
    16801 caccgcacca aagaacagtc ccgaggcgcc gagcaacacg gagcgcaggt cgaacaccag
    16861 gccgggctgc agctcgaagg gaaacatcat gatggcgacg ccgaccaggc cgagcagcag
    16921 cccgacgacc acgctccagg cggggcggga cctgcgcgcc cgccgactga tcgctagcgc
    16981 aaggatgtgt gcgacggaga tcagcagcgc cgcgttcagt accagcgccc cggcagtcgc
    17041 gaactcgagg atcacgattc ttcctcggga agctcgatca cccggcgccc gaagggcggt
    17101 ttcagtccga gccgcacgga cagctcgttg acctgctgct tcagccgcag catgtccaac
    17161 tcgcgaccga ccattgcctc gttgaagcgt tccagttcct ggtttcgttc ctcgagctgg
    17221 cgcccgtgtt cctcgagcag tatggacgaa tgctccaggt ccgtgacgtc gcgcgagatt
    17281 ccgtacacgc cgatcagtcg accggaatca tcgtggagcg ggccctttct cgccaggacg
    17341 gcgcgttgcc catgggccgt gtggagaatt tctcgcgcca tcactgcccg gttctcggcc
    17401 agcacgcgac ggtgggtggc cgccagcatc tcggcctgtt ccggcgggaa cacggaataa
    17461 tcgtcccggc cgaggacctc gtcctcggac ctgccggtca tctggcaggc ggcccggttg
    17521 aacaacaggt aacgtccgtc ggggtccttg acgaagatcg cgtcctcggc gctgtcggcg
    17581 acggccttga gaagctggag cgactgcagg cgctcgccct ggaccttctg gatagtacgg
    17641 gcctggcgca ggtgctggcg ctgccgccgg ttgaccagtg tcgcgccgac cgccgagaaa
    17701 cacagcaagc tgagcaaggc gatcggcagc atgccgcgca gcgcggcccc ggccacctcg
    17761 ctgatgtcca ttttggcgac caggaaccag tccgtgcccg ggatggcgcg gccggagccg
    17821 aacaccgcct cgccacggta gtcgacgccc tcgacccgcg cacgatcggc gggtcctgcg
    17881 cctgcgatct tcaccgccag ctggtctggc gcctcgctca tcacgagccg cgtgaacgcc
    17941 gatccctcga catgcctgag gtcgtgcagc gcgaccacgg cgtcgccttc gacacggaac
    18001 agcaatacct ccccgctggt gctcggcaag ggaaaggatc gcagcgactc ggggaagtag
    18061 tcggaggccc cggtgtgaag caggaccagg ggtgtgctct cgctcccttc caccggcagg
    18121 ctggccagga aatcgaggtg aatactgcca tgttcgtcct tgaacgggcc gatgaagccg
    18181 gcgcccctgc ggtgcgccga catcatggcc gcgtccagcg actcggccgg cagttgcagg
    18241 gtcgggtggg cgtctcccgc gagcggccga ccggcgccgt ccagcaagtt cacggcgtaa
    18301 aagcgcccgg agtccgcgaa ctgcttcagt cgcagcaaca gcaggtcccg ggcgtgcaga
    18361 tccccctcga cctgccagcg ggtgtacagt tccgcctgcg gaaaacttgt ggcatgcagc
    18421 ccggcgctgc gcaggcgctc ctcgagccag ttggcaacct gcgtgctgtg tgattccgcc
    18481 accgaccaaa cgatggtcca gtagtcggcg cgtttctgct ggtagacatg gttggccatg
    18541 accaggttgg ccagcagcag cagggtcccg agcacggcca ggggcagcca gaggctgcgc
    18601 cgtgcggccc tgtcgtcgcc gccggcgatc gagaccgggg ctggccgggt ttccgaaggg
    18661 tgcatgggca ctcgtcgcca agtggcggac gtgaatttgc tgcaccgtaa cacccggctt
    18721 tggccgcagt gttgaatcac gtctcattag ggtaaattcg attcatctaa tgcgttgccg
    18781 accgctgtga ttccatgtcc attttcaggt cgcggcggct cgcggaacgc gctaacttag
    18841 cgccatggaa tcgcacccgc acacccatcc gcaggccacg cccctgccgc cgccggacga
    18901 aatgtatcgc gcgctggtgg cgcgtgatcc gggctacgac ggcatcttcg tcgtgggcgt
    18961 ccgctccacc ggaatcttct gccgcccggt ctgcccggca cgcaagccgc gccgggagaa
    19021 cgtcgatttt ttcgccggcg cgcgcgaggc gctcgcgcac ggcttccggc cctgcaagcg
    19081 ttgccggccg ttgcagccga gcggcgcaac gccggccgcg atcgctggcc tgctggcgga
    19141 actcgatgcc gatcctgccc tgcggctgcg tgacggtgac ctgcgcgtgc gggggctgga
    19201 gccggccacc gtgcgacgct ggttcaagca gcaccatggc atgaccttcc acgcctacca
    19261 gcgggcgcgg cgcgtgggcg ctgcgctcgg cacgctggcg caaggggcag gcgtcacgga
    19321 caccgccttc ggcagcggct tcgactccac cagcggcttc caggaagcgg tacggcgcat
    19381 caccgggcgc tccgccgcag ccagccgcaa tgcgccactg gtgcgccttg ccagggtgtt
    19441 gacgccgctc ggcccgatgg tcatgggcag tacggatacg ggcgtagtgc tgctcgagtt
    19501 cactgacagg cgcatgctgg agacgcagct gcaacgcctc gcgcgcaagc gcaagtgcgc
    19561 tttcgtgccc ggccagaccc acgcgggccg ccaactggag caggagctgg ctgcttattt
    19621 cgagggccgg ctgaccgcct tcgagacgcc gctggagccg gccggcaccg agttccagcg
    19681 ggccgtctgg cgggtgctca aggacattcc ctacggccag acgcgcagct acggccagca
    19741 agcgacgtcg ctcgggcgcc cgagcgcggt gcgggcggtg gcgcgcgcca acggcgacaa
    19801 cccgatcgcc atcgtggtgc cctgtcatcg cgtcatcggc gccgacggca cgctgaccgg
    19861 ttacggcggc ggcttgtggc gcaagcgctg gctgctgcac cacgaacaga gccggctggc
    19921 cggccgcgta ccggcccgct gacacgacgc gcaacgtgcg acatcacctc gccgcggtcg
    19981 cgcttgcgcc ggtgctgctc gtacagggac tgcgggtacg ccgtgtcacg ccgcggttgc
    20041 ccgaaccgcc gggtccgcgc tcgggtatcg cgggcagcgg gtcgccactg cggctgctga
    20101 tcgcgggcga ctccgccgcc gcgggggtcg gtgcagagac ccagtcacag ggcttgtccg
    20161 gtgtggtggt cgaagcactc cgccgggatt atcgcgtgtc gtggtccttg attgcccgta
    20221 ccgggctgcg caccgccggg gtcatcgagc ggctcgaggc ggcggccgtg gaaccgttcg
    20281 acgtcgcgct gttgtcggtg ggcgtcaacg acgtgaccgc attcacgccc gtcgggcagt
    20341 ggctggcaca gcaggcgcga ctggtggcgc tgctcaagtc gcgattcggc gtgcggcacg
    20401 tgctgctcac ctgcctgccg ccaatgcatg cttttcccgc gctgcctcag ccgctgcgct
    20461 ggtacctggg tgcgcgggca cgcacgctca atgaccacct ggtggccttt gcggctgccg
    20521 acccggcctg cgaggtggtg gctccggatc tgccgcctga tccggccctc atggccgcgg
    20581 acggctttca tccaggcccc ccggcgtatc tcgaatgggg ctgcagcgcg gcgcggatca
    20641 tccggcgccg ggtacccagc gggccgactt gggcttaaac tcccgcattc atcgcgggag
    20701 tcatcggcat gaatattgac ctgagcatct ccgcagccgg cgtctgggcc ctggtgggcg
    20761 tggtgctgat cctcgcggag ttcgccatga ccggcgtgat cctggtgttc ttcggcgtcg
    20821 ccgcgctgct cgtcgccctg ttgctctgga ccggcgtgga catgtcgctg aacgtccaga
    20881 tcctggtgtt cggcgcgctg gccatcggcc tgctgctcgt tgcgcgggac cgcgtcaggt
    20941 catggttcgg cggcaagtcg gagcaggccg gcgacggcat cgaggtgctg gtacccggca
    21001 cgccggtgac cgcactcggc gatttcgtcg acggcgtcgg gctggtgacg catcgcggcg
    21061 cccgctggaa tgccgagtgc gacgccccgg tggcggccgg ccagcgggtc tggatcaccg
    21121 gtcgtcgcgg cctggtgctg tcggtcagca ccgagcaaaa cgccccggga cgctaggcgt
    21181 cccgatccac ttcgaatcga gccggccgaa ggagttcacg tgcaaaccgt agacctgatg
    21241 acgatcatct cgctggtcgt ccttgccatc atcgtcgtgg cgctgttcaa gacggcgcag
    21301 atcgtgccgc aaagatcggc ctacatcgtc gagcgcctcg gtcgttatgc gcgcaccctc
    21361 gatgccggct tccacatcct cattcctttc atcgaccggg tggcctaccg ccagacgctc
    21421 aaggaagagg ccatcgacgt gccgaagcag cagtgcatca cgcgcgacaa catcagcgtg
    21481 tcggtggacg gcgtgttgta cctgcaggtc gtcgatccgc acgccgccag ctacggcatt
    21541 gccgactatc actacgccac tgccagcctg gcccagacca cgctgcgttc ggtcatcggc
    21601 cagatcgagc tcgacaagac cttcgaggag cgcgccaaga tcaacgaaga ggtggtcaag
    21661 gcgctcgacg aggccgccaa cccctggggc gtcaaggtgc tgcgctacga gatcgccgac
    21721 atcgtagtgc cgacgacgat caccgacgcg ctcgagcagc agatgcgtgc cgagcgcgaa
    21781 cgccgcgcgg tggtggcgcg ctcagaaggc caacgccagg agcagatcaa cgtctccgag
    21841 ggcgagaaga tccagatcat caacctgtcg gaagcgcaga agcagaaaca gatcaacgag
    21901 gcggagggca aggcgcgcga gatccagatg ctggcggagg ccaccggcca gggcatcgag
    21961 cgcattgcga ccgccatcaa tcagcccggc ggcaaggagg cggtgagctt gcgcattgcc
    22021 gagcagtacg tgcgcgagtt cggcaacctg gccaggacca acaacaccat gatcctgccc
    22081 gccgagctca gcaacatcgg cgccgcagtg gccgggctgg cggaggtgct gaacaaggcg
    22141 aagccggggt cggcaggcgc cggctagccg cggtgtgagc cggtcgagct ggacccgagg
    22201 tcggcccggc acgaacggac cttgtcacgg tcgagtgggt gaccgataat tgtgcggtgc
    22261 aatgacacgg acaacggggt cacccgttgt ccgtttgtca ttccggcaca ggacgttcca
    22321 ttcgcaccaa gagagatttt cactatgaac gcaaagttgc tggccctggc cggcctgctg
    22381 gcccttccgg gcgctgccct cgccacggac ctgaattaca actacatcga ggcggactac
    22441 gtgaccatcg acgtcgacga tatcgacgag gatttcgacg gctgggcggt caacggctcc
    22501 ttcctgatca ccgagcaggt cttcatattc ggtggttact ccgatatttc cagtgaccgt
    22561 atcggcggct tcaagctgag caccgaggga gtgaccctcg gcctgggcta tcgccatgag
    22621 ctcacccggc agaccgacct gaacttcgcc ggcgccttcg aacgcgccaa ggccaaggcc
    22681 gagctgggcg gtttcggctc ggagtcggtc agcgagaacg gttattcgct ggcggtgggc
    22741 gtgcggcacc tggtgtcgcc gcagcttgag ctgggcgtcg gcgcagtgta cgtggatatt
    22801 ggcgatggcg acgacaccaa cgccaccgcc aacgcgctct ggcacgtcac ggaagccgtg
    22861 gccctgggcg tgaacctgag cctcggcagc gacgcgacgg gttacggggt cggcgtacgg
    22921 ctcaagttct aagcaggctg ctgctttcgc agcaggttcg aacggagcgg cggggcgtaa
    22981 atccccgccg ctttttcgtt tccgccgccg gcaatccgag gcgggtaaga tgtctcccct
    23041 gggatgtgcg ccgggagcgg tacgcgtcat gcgattcatc catacggccg actggcacct
    23101 cggacgccag tttcacaacg tgtcgctgat cgacgaccag cggcacgtgc tgcggcagct
    23161 gctggatgcg gcggtgggag ccgaggtgga cgccgtcgtg gtggcgggcg acgtgttcga
    23221 tcgtgcggtg cccagtccgg aggccgtcga gctgctcgac gagcacctcg ccgagctggt
    23281 gctggcccgg ggcattccgg tgatcatgat cgcgggcaac cacgacagcg gccgacgcct
    23341 cggcttcgcc tccggcctgc ttgcgcgctc cgggctgcat gtctggggac gttacgaggg
    23401 ccggccgccc agggcggttt tgtccgatcg gcacggcgtc gtgcatttcc tcgggctccc
    23461 ctacgccgag cccgcacagg tacgcgagct gagcggcgac cctgggctgc acagccatgc
    23521 cgccgccatg cactggctga cccgggcggc gctcgagtcc gtgccggcgg gcgagcgcag
    23581 cgtctgcgtg gcgcactgct tcgtcgcggg cggggcggag agcgagtccg agcggccgct
    23641 cagcgtgggc ggcgctgccg ccgtgcccgc cgagtgtttc gccggcttca gttacaccgc
    23701 gcttggccac ctgcatcgtc cccaggccgt cggcgaacgg ctccgctact ccggctcgct
    23761 gctgaaatat tccttctcag agattctgca tcggaaatcc gccgacctgg tcgagctgga
    23821 tgcgaccggc ggggcaacgg tgacgaccct cccgctgcag ccgctgcgcg atctgcgact
    23881 cctcgagggc gagttggcga ggctgctgga ggggccctcg ccgggcgaga acccggagga
    23941 tttcctgctc gtgcgcctca gcgacgagca cgagctgctc gatcccatgg cacggttgcg
    24001 cgaaatctac ccgaatgtgc tgcacatcga gcgcccggca ttgcagcggg cgggcgagga
    24061 cgccttgcga gcgcatcgcc ggcagggcag cgacgtcgag ctgttcaatg ccttttacgc
    24121 gcaggccgcc ggcgtacagc tcagcgaggc gcaggccggc gctttcaggg aagtctacga
    24181 cgcgctgctt gcgcagagcc gggaggcctc ccggtgaggc cgctcaggct cgagctgacg
    24241 gcattcgggc catatgccga gccgcaggtc atcgacttcg gcgcgctcgg tgagcgccgc
    24301 ctgttcctgg tgcacggtcc gacgggcgcc ggcaagacga ccctgctcga tgccatctgc
    24361 ttcgccctgt acggcgacac gaccggcgcg gagcgcgacg gcaggggttt tcgcagccag
    24421 tttgcaggac cgacgctgga gaccacggtc accctcgatt tccagctcgg tccgcacgtc
    24481 taccgggtga cccgccagcc ggagcaggag cgcgccaagc gcgtcggaca gggcacgacg
    24541 gtctcgccgc cctccgcaac gctgtgggac cgcaccgaca tcgaggaccc ggcagaggag
    24601 ggcagggtgc tggcgacgcg cttcggcaac gtcacggcgg cgatcgtcga tctgctgggt
    24661 ttccgcagcg agcagttccg ccaggtcatc gtgttgccac aggggcggtt ccgggacgtg
    24721 ttgctggccg gctcgaggga gcgcgaggac atcctgcgcc agctgttcga caccgggttc
    24781 tacgagcgta tcgaggaggc gctcaaggag cggagtcgtg aactgcgggt ggcggcagag
    24841 cagctgcgtg ccgagcgcac ggcgctgctg aggccggaag agctcgaaga cgaggcggcg
    24901 ctagaggcgt gcctgcaggc gctggcggcc gagaagcgcg acgtcgaccc gatgttgagg
    24961 tccttgcagc aacgaagctc ggccgccgcc gaggcgctgg agcgcgcccg cggggagcgg
    25021 gcgcgcttcg agaaggcgga acgggcgcag cgcgcgcttg ccgagttgca ggagcgcagc
    25081 gcggagatcg acgcggaccg gcagcgcctg caggcggccc gtcgcgcagc gggcctcgtg
    25141 gacctggccg cgcagctcgt cacactacgc cgctcggaag tcgaatcgcg cgggctgctc
    25201 gaggacctgc gtgcgcgccg gtccagcgcg tcggagcggc tggagcgcgc ggcgaccgcc
    25261 gctcgcgccg agcaggcgcg gtcacacgag cgcgatgcgc tcggccagcg ggcggccgag
    25321 ctggcgcgat tgctgccgct ggccgaggaa ctggcaaagc tcctggaacg ggaatcggct
    25381 gaaacggtcg cgcagcaggc tgcggctgag ggcgtggagg ccgccatgga agccgcggcc
    25441 gcggctcgcg ctgatgcggc agtcctgcgc ggcgcaaggg aatccggcag cgcggcgctg
    25501 ctggccgccg agctgcggcc tggcgagccc tgcccggtgt gcggttcgac ggaacatccg
    25561 cggccggcgg ccggcggtgg cgagattcca tcgggcgagg cgctggcggc ggctgacgag
    25621 gcgatcgctg cggcggaggc gactctggac gagcaccggc aagcgagcca gcgcgcggcc
    25681 gcggtcctgt cggatctgcg cgcgcgcatc gataccttgc gcaggcaact ggagggcgtg
    25741 gtcgaaccca cgccagcgag gctgcgggag ctccctcgac tggcggagga ggccgtgcgg
    25801 cggggcgccg ccttgcagga ggcgctcgat cgggcgatgg cacaggagca gtcggcccgc
    25861 gacgagtctg cgcgccttga agcagcgcac caggccgcca tggtgcagga cggcgccgcg
    25921 cgcaaggcgc tgcaggcggc cgaaaaggaa tggtcgctgc gcctcgacca ggcgggcttc
    25981 gacagcgagg cggcgtggcg cagtgccgcg ctcgaggagg gggaaatcga gcgcgtccag
    26041 acccaaatcg atgaattccg gcaacggctg gctgcggccg agggcatggc ggcagactcc
    26101 cgggcggagg tggcggggcg agccgcccga cctcgatgca gcgaaggtcg tggcgaccgc
    26161 ggctgcggaa gagtacggcg cggccgccac gcggagcggg cagctggcca gccggatcga
    26221 cgcgctggaa cggttgcgcg cgcggctaca ggacattgct gcggaattta cagcgacgga
    26281 gcagcgcttc ggcgtgttcg gcagcatcgc gcgggtcgct gcgggcgaca acttgcaccg
    26341 tatttcgctg catcgcttcg tgctcgcgag caggctcgac gacgtgctgg cggctgccag
    26401 ccggcgcctc cacgccatga ctcgtgggcg ctacctgctc aggcgcaaca ccgagaccgc
    26461 agaccggcgc gccgccggtg gcctggaggt actgggcgag gacgcctaca ccgccagcag
    26521 ccggccggtc gcaacattgt ccggcgggga gagcttccag gcggcgctgg cactggcgct
    26581 cggcttgtcc gaagtggtac aggcctactc gggcggcatc aacctggaca cgatcttcat
    26641 cgacgaaggc ttcggcagcc tggacccgga ggcgctcgac ctggcgatcg acaccctgat
    26701 ggacctgcag cagtcgggcc ggctggtcgg agtgatttcg cacgtgccgg aacttcggga
    26761 acgtatcgac gtccgtctcg aggtggtggc gggcgctgcg ggcagccgcg ccgcctttca
    26821 tttaccctga gccgatcggc gagaccgcgc gcatgctcac gaccatcgtt ccactgaccg
    26881 ccttgctgtc cggcgcagga ctgctgctgt tgggcagcgg cctgctcggt accctgctgg
    26941 gcgtgcgcgg cacgctcgag ggctatgacg accgggcgct cggcctgatc atgtcagcct
    27001 actttctcgg cttcttcctc ggcaccttcc tcgcgccggc ggtgatccgg cgcatgggcc
    27061 acatccgcgc cttctcattt ttcggcgcgc tgttcgcgag ttcggtgctg ctgcacgcca
    27121 tgtgggtgga tccgctcggc tggggcctgt tgcggatcgg caccggcatc gccgtggtcg
    27181 gcctgtacac ggtgatcgag agctggctga acgccaccgt ggacagcacc cagcggggcc
    27241 gcgtgttcgc ggtctacatg ggcatcaacc tggtagcgct ggcgctcggc cagcagctgc
    27301 tgcgactggc gccggccgag tctttcgcgc tgttctcgct cgcggccatc ctggtctgcg
    27361 cggccatgct gccggtcacg gccacccgcc tggtgcagcc cgtcatgcag ccggccaccc
    27421 gcatcggtgc gcggcagctg ttcggcatcg cgccggccgc cgccgccggc gccatgttct
    27481 ccggcctgag cgtcggcgcg ttctggggac tgggtccggt gtacgcgggg cggctggagc
    27541 tgggcatcga aggcattgcg acgttcatga cgatgaccat catcggcggt gcggcgctgc
    27601 agtggccggt cggccggctg tcagacagcg gcgaccgtcg ccgcaccctg gcgctggcct
    27661 gcatcggcgc cgccatcgcg gccggccttg ccgtgtggta ttcgggcgcc tcggcctggc
    27721 cgttgtacct gttgtttttc atgtacggcg ggctgtcctt cgccgtctat ccggtcgctg
    27781 tggcgcacct tctcgatcac gttccgccgg aggagctgct gcccgcgtgc agcaccgtcc
    27841 tgttgctgca cggcgtcggc gccgccttcg ggccggcgat cgccggcttc gccatggcgt
    27901 ggctcggggc gcaggccttt cccggctacc tgatgctggt gcacctgctc ctggccgtct
    27961 acgtgttcct gcgcctggcg gcccgccagc gcgtcgccca gggagagagt catttccacg
    28021 ccatgctgag gacttcgccg accgccatgg agatgctgcc ggaggtcgaa gccgcgcagg
    28081 acgaagcctc ctgaatggct agaatgccgc gcgccaggcg aggtgcagga ccaggtcggg
    28141 cgtcgtgtcg gtgaacaggt tttccaccag caccaggtcg agccggccgg cctgcgccag
    28201 catgatgctg ccaccgaagc tcagttgcac gccggccgag ccgagcggct ccagttcggt
    28261 gtcatagaag gacgaatggc cgtcgatctg caggcgcaaa tcgaggcggc gccacacggg
    28321 acgcgagaca ccgatgctgc ctaccgccac gaggtcgcgg cgcaagccgc cgagcacgtc
    28381 gccgtcgccg agccagagtc cgccgatcat ccatgccagc gtcgtgttcc acgccgacag
    28441 cgtcgctgcg tcggtgacca gcaactggag cgagacatcg gtactgccgc tgccatgcag
    28501 gtcgtcgctg ctgccggtcg gcagcttcag gccgccgcgt aatcccagcc gccgcctcgg
    28561 atccgcggtg gcgaggctgc ggtcgagcgt caggcggaca tcacccaggc cgcggctccg
    28621 ctcggtcagc tcgaatgcct ggccggcccc gttcgtatag gagtactcga gcgcgtcgtc
    28681 ccggaattca tcccgacgac ggttgctgag gccgaagagg tcgtgccagt cgcgcacgaa
    28741 gccgtcgagc aagccgccgc tgtgggcgac gaatggaatg ctgagcccgg cgacgacgcg
    28801 ctcggagagc tggcggcgca acaacaggct cgtgcgatag gtttcgccgt cgagcgccag
    28861 gcgctcgccg gacggcagtt cgtcgcggat ggcgccattg gcgacgtcga gctcgatacc
    28921 ccaggtccag cggtccgtcc cgggtgcgat gtgcatggcc ggcgagggca ggctgtaaat
    28981 ctggatgaag gggttctggt cggtggtggc gaacaggccg tgcagctcgg gcgacgcctc
    29041 cgcctggagc ggggccagcg ccgcggccat gtacaatgcg gccctggcca ggaccggcgc
    29101 cgggagtcgg cggcgggcgt cgacagaaga attcaaggag ttcacgaggc ttcggcatcc
    29161 agcttggcat ggcgcctgcg gcgccgttcg ccgaagtctt ttatctgatc ggggggcggg
    29221 ttgccagttt tcgcgacaaa tgaaacgcgt ggtccaggcg cggcggtact gttgctgtgc
    29281 ggcgtcctgt cgggctgggc cggcgtggcc ggcgccgagc agcacgctcc gccacacgcc
    29341 ggggcatcct gggcctggag caatgcgacg ggcgtccagg gacctgtcga tccgggcgaa
    29401 gattcatggc cgccacgcga caggaatccc gccgggctgc gccgtgacac cgcctacctg
    29461 ctgtcctacc aggtggtgac catcggcatt ctctacgccc tgcccgagag cgtcagcaac
    29521 tggagcgacg aacagaaaga aagctacagc ctcgccgact ggtgggagaa cgtcacgaat
    29581 ccgcaatggg ataccgacga ctactacctg aactacatcc tccatcccta ctggggggcc
    29641 ggttacttcg tacgggcgcg cgagaatggc tacgatgagc gggcctcgtt ctggtactcg
    29701 gcggcgatgt cgaccgcctt cgagttcggt gccgaggcgc tgttcgagca gccttccatc
    29761 caggacctca ttgtcacccc ggtcgccggc gccatcctcg gcgagtattt catgcacctc
    29821 cgtgccggca tcctggagcg ccgtgatccg ggcgaaccgc tgccgctgga agaccgcctc
    29881 ctgctggtgc tcaccgaccc gatcggggcc atcaaccgcc aggtccagtc ctggctcggg
    29941 ctgtcggacg cggccgcgat ccagccttac tacgccccgc aacgggtggg cgccgtgcgg
    30001 ccctatgctg tgcccgacac cccggtcgac tgggtgtttg ggctgcgcgt tgattaccgc
    30061 tggtaagacg cacataagca attgatctgg ctgggctgtc cagcgcctct ggcatctcgt
    30121 gccgcattgc gacaataatc gtggcagtca cagccggcca atcttcggag cgcacatgag
    30181 caagcacagg ttcgagaccc tgcaggtcca tgcggggcag gcgccggccg cgggcacgta
    30241 ctcgcgcgcg gtgccgatcc accagacgac gtcttacacc ttcaccgacg ccgaccatgg
    30301 tgcgcggctg ttcgccctgc aggaattcgg caacatctac acgcgcatca tgaacccgac
    30361 gacggacgtg ttcgagcagc gcgtcgcggc gctcgagggc ggggtggcgg cgctggcgac
    30421 cgccagcggt cagtcggcac agttcctcgc catcacgacc ctggccgagg ccggcgacaa
    30481 catcgtctcc ggcgccagcc tttacggcgg cacctacaac cagttcaagg tccagttccc
    30541 gcgcatcggc atcgatgtgc gcttcgtcgc cactgacgac ttcgacgcct gggaggcggc
    30601 catcgacgac cgcacccgcg cgttgttcgt ggaaagcatc ggcaacccgg gcttcgacgt
    30661 gccgcatttc aggaagctgg ccgagcttgc ccggaagcac gagctgccgc tggtggtgga
    30721 caacaccttc ggctgcgccg gttacctggt gcgtccgatc gagtacggcg ccgacatcgt
    30781 cgtacagtcc gccaccaagt ggatcggcgg ccatgggacg tcgatcggcg gcgtcatcgt
    30841 cgacgccggc aaattcaact ggttcggcaa gcggttcaag accttccaca cgccgtcgcc
    30901 gggctatcac ggcatcaatt tcgccgcggt cttcagcccc gacgggccct tcggcaacct
    30961 ggccttcatc atccgcgcac gggtcgaggg cctgcgcgac tgggggccgg cgctgtcgcc
    31021 tttcaacgcc ttcatgttcc tgcagggcct cgagacgctg tcgctgcgcg tggagcgcca
    31081 ctgcgacaat gcgctggagc tggcccgctg gctgaccacg cagccgctgg tggagcgggt
    31141 gaattacctg ggcctcgaat cgcatccgca tcaccagcgc gcgcgcgagt acctgcgcca
    31201 cggcttcggc tcggtgctga ccttcggcat ccgaggcggc ctggaagccg gcaagcactt
    31261 catcaactct gtggagctgg ccagccacct ggccaacgtg ggcgacgcca agacgttggt
    31321 catccacccg gccagcacca cgcatcagca gctctccgcc gacgagcagc tctcggccgg
    31381 cgtgctgccg gacctggtgc gggtatcggt cggcatcgag cacatcgacg atatcaagga
    31441 agatttcacc caggccttcg ccaaggtggc gagccggggc ggatgagcaa gtcattgctg
    31501 ccggcggcgg cgccggtctc gaaggacacg cgactgtggc gccacgacgg gccgttctgc
    31561 cttgaaagcg gcgtcacgct gccggcggtc caggtcgctt accgcacgtg gggccaactc
    31621 gctcccgacg ggagcaatgc cgtgctggtc tgccacgcgc tgaccggctc cgccgacgcg
    31681 gacgtgtggt gggagggatt gttcggcgca ggcaaggcgc tggatcccgc ccgcgacttc
    31741 atcgtctgca gcaacgtgct ggcgggttgc tacggctcga gcggcccgat ccagccgcat
    31801 ccggaggatg gcgagccgtg gggcagccgc tttccggtcg tcacgatccg cgacatggtg
    31861 cgactgcagt cgctgttgct cgatcagctc ggggtcggcg agctgcagct ggtgctcggt
    31921 ccctcgctcg gcggcatgca ggtgctggaa tgggcggcca gctatccgga gcgggtgagg
    31981 gcgatggcgc cgatcggcgt atcgggccgg cattcggcct ggtgcatcgc catcagcgag
    32041 agtcagcggc gcgccatcta cctcgatccc gactggcggg acgggcgtta tccgctcgac
    32101 cggccgccgc gctacggctt gtccgtggcg cgcatgatcg ccatgtgcag ctaccgcagc
    32161 tgggacaatt tcgaggcccg cttcggcagg aagacgcagg agtgggacgg gcagtttgcg
    32221 gtcacctcct acctgaatta ccagggcgaa aagctctaca agcgcttcga cgccaatacc
    32281 tacgtgcggc tcacgcaggc catggacagc cacgacctgg cgcgcgggcg cggcgattat
    32341 gccgaggttt tgggtgggtt gcggatgccg gcgctggtcg tgtcggtgga ctcggacgtg
    32401 ctgtatccgc ctcatgagca ggccgagctc gcacggctgc tgccggcggc acgctacgag
    32461 atgctgcaca cgccggccgg ccacgacggc ttcctgatcg agacggatgt gctcagtgac
    32521 atgttgctga gctttcgttc gtcgtagcgg cgtcgcccgc acccggctcc cccggttcaa
    32581 agcgctccag gaagaaacac aactgctcgg gctcggagct gaaccccagc ccgcagttgc
    32641 gagaatccct tactggcgcg ccgcgactaa agtggtattc tctaagaatc aattcatcgg
    32701 aggcgatgcg ggacagcccg tattgccgtc cgcagccgca cccggccggc gtgccgggag
    32761 gagtccggcc gtgtgcctcg cagtccctgt tcgcgtggtg gcggtgccct cgccggagtg
    32821 ggccatcgtc gacctgggcg gcgtggagag ccgggtctcc atcgtcttcg tcgacgacgt
    32881 gacgcctgga gattacctga tcgtgcacgt cgggacggcc atcgcgcgac tggatgtcga
    32941 gcaggctgag gagtcgctgg cgctgttccg ccagatcgcc gagaccgtcg gcatcgagac
    33001 tgatgcgcta catacgcggc ttccgtgacg gccgggcggc gcgcggtctc gcgcgcctga
    33061 tcgcggccga aacgcggtct gaccgcgagt accacctgat ggagttctgc ggcggccaca
    33121 cccacgccgt gttccggcac ggcctgccgg acctgctgcc cgacaacgtc cgcctggtgc
    33181 acgggcccgg ctgcccggta tgcgtgctcg ccgtgccgcg gctggacctc gccatcgagc
    33241 tggccttcga cgagcgcgtg atcctcgcga gctatggcga catgttgcgc gtgccggcct
    33301 cacgcaagaa gagcctgctc aaggcccgtg ccgcgggccg tgacgtacgc atcgtgcagt
    33361 cggcgctgga tgccctcgac atcgcgcagc ggcatcctga gcgggaggtc gtgttcttcg
    33421 gcatcggctt cgagaccacg acaccgccga cggcgctggt gattcgggag gccgaaaggc
    33481 gcggcctcga gaacttcagc gtttattgca accacgtgct gacaccctcg gcgatcctga
    33541 gcatcctgga ctcacccgaa gtccgcgaac tgggccaggc gcgcgtcgac ggcttcctcg
    33601 gtccctcgca cgtcagcacg gtgatcgggg cgcgccccta cgagtatttc gccgaggagt
    33661 atcagcgccc ggtggtcatc gccggcttcg agccgctcga cgtgttggcg gcggtccgca
    33721 tgctgatccg ccagctgaac gagggacggg ccgaggtgga gaaccagttc acccgaggcg
    33781 ttacccgcga cggcaatctc aaggcccagg ccatggtcgc ggaggtattc gagctgcgcc
    33841 cggagttcga gtggcgcggg ctcgggaaag tgcagtacag cgggctcagg atcaaggccc
    33901 gctatgcccg cttcgacgcc gagcgccgtt tcgacctggt cgagaagccc gccgtggaga
    33961 acaaggcctg ccagtgtccg gcgatcattc gcggcctgaa gaagccgacc gactgcccga
    34021 ttttcggcac ggtctgcaca ccgcggaatc ccatcggtgc gtgcatggtg tcttccgagg
    34081 gcgcgtgcgc cgcgtggtac cagtaccgca acgggctggc cgagacggcc tgaggacgac
    34141 tgggtgcgca tcctgattct cacgcattct ttcaacagcc tggcccagcg gattcatgcc
    34201 gagctcgcgc tcctcggcca tgacctgacg gtcgaactgg acatcagtga cgaggtgact
    34261 gaatccgcgg tggcgctgtt tcgtcccgac ctggtcgtgg cgccgtttct caagcgcgcc
    34321 atcccgccgt cggtctggcg gcgcgtgccc tgtttcatcg tccacccggg aattcgcggg
    34381 gaccgcgggc cgtcggcgct ggactgggcg attctcgagg ggcgcgagac ctggggcgtc
    34441 accgtcctgc aggcggaggc cgagatggac ggcggcccgg tctgggcctg gcgcgagttc
    34501 ccgatgcgcc tggccgcgaa gggcagcatt tatcgtttcg aggtgaccga ggcggccgtc
    34561 gccgcggtgc tcgaggccgt cgacaagctg gcggagggcg acttccgtgc ggaggcgccg
    34621 gacccggccg atcgcgcgat cgccggcggc tggcgcgatt ccatgcgcca gtcggaccgg
    34681 cggatcgact ggcagcagga cgacacgatc aaggtactgc gcaagatcca ctcggcggac
    34741 ggccaccctg gagtccgtga caacatcgcc gggcggccgg tgcgcttgta tgcggccagt
    34801 gccgagtacc ggctggcggg cgcgcccggt gaactgctgg cgcagcgcga cggcgcggtc
    34861 tgccgcgcga ccgtcgacgg cgcggtatgg atcggacagg ttcgggccga ggctg
//
LOCUS       DAOMPG010000003        30075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_682_length_30075_cov_4.466322, whole genome shotgun sequence.
ACCESSION   DAOMPG010000003 DAOMPG010000000
VERSION     DAOMPG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 30075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30075
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_682_length_30075_cov_4.466322"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLS34_00300"
     CDS             complement(<1..859)
                     /locus_tag="PLS34_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP85942.1"
                     /translation="MVQDTVARFTDEKVLPIIRECFEEHRFPAELVPEIAALGLLGSS
                     LEGYGCAGLNGVSYGLICQELERGDSGIRSFVSVQSSLVMWPIYTYGSEAQKQRWLPA
                     LAAGEAIGCFGLTEPHGGSDPGNMKTHAKRDGGDWVINGAKMWITNGSVADVAVVWAI
                     TDEGLRGFLVEKGTKGFDAPEIHRKFSLRASVTSSLFLDNVRVPAENMLPGVVGLKGP
                     LGCLTQARYGITWGAIGAAQACLKEALDYSNDRELFNRPVSHTQTVQRRLADMARRIT
                     TAQLLSLQLG"
     gene            1032..2357
                     /locus_tag="PLS34_00305"
     CDS             1032..2357
                     /locus_tag="PLS34_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRP85943.1"
                     /translation="MKIPEILLIESEHRPGSLASILAVIGEQGLLVEHLAALRREQDK
                     TVWEITLEMDPAAGRRLEERINALPNARVLGRSDRVFNRHRGGKIRTVSTLDIGSLQI
                     LRDVYTPGVARVCLAIRDQPELAREFTAIGRTVAIVTNGTAVLGLGDIGALAGMPVME
                     GKAALMASLAGLSAVPILVEEKDPARLTGVIAAIAPSFGAIQLEDIAAPACFEVEDAL
                     RAQLDIPVLHDDQHGTAVVALAAVRNAGRQAGQRLSQSTVGQVGLGAAGIGIARLLLS
                     RGTKRILGADLNPAARDRFAALGGEPAELARVMAEADIVIATTGVKGLIRPESVRPGQ
                     VILALSNPDPEIEPQAALAAGAAFAADGKSINNVLGFPGLFRGALDAGARRFTDEMLV
                     AAAESIADLAGEHDLVPDPLNKAVHDAVARAVAGAAASSGACQAAGVAQ"
     gene            complement(2351..2818)
                     /locus_tag="PLS34_00310"
     CDS             complement(2351..2818)
                     /locus_tag="PLS34_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002153811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HRP85944.1"
                     /translation="MHDLESWLDEYGESHQHPANKLIHWICVPAIVFSVIGLLWSLPV
                     PGAFADISPALNWALLFSMASVVYYFILSLPLGFGATLVLAVLLWLAAVLESAAAPLW
                     AVSLAVFVAAWVGQFIGHRIEGRKPSFFKDLQFLMIGPLWLLAAAYRRAGIPY"
     gene            2926..3597
                     /locus_tag="PLS34_00315"
     CDS             2926..3597
                     /locus_tag="PLS34_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP85945.1"
                     /translation="MNKTTARIVMLAAAAAMTVGCQTIDPYTGETKTSSATKGAAIGA
                     GAGAVLGILTGDDSKERRKRALIGAGVGAIAGGAVGSYMDNQEAKLREQMEGTGVSVT
                     RVGNDIILNMPGNVTFQTNSSDLNPQFFGVLDSVGLVLKEYDKTVIEVVGHTDSTGSA
                     EYNQALSERRAATVATYINNRGIDRQRILAFGRGLTQPIADNSTAAGRAMNRRVELTL
                     SPITN"
     gene            complement(3613..4485)
                     /gene="rpoH"
                     /locus_tag="PLS34_00320"
     CDS             complement(3613..4485)
                     /gene="rpoH"
                     /locus_tag="PLS34_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRP85946.1"
                     /translation="MSNALVARQNELMLTGPVGNLGAYINAVASIPVLSAEEESELAW
                     RFRRDNDLDAARQLVLSHLRFVVHIARGYNGYGLQLGDLIQEGNIGLMKAVKRFDPSV
                     GVRLVSFAVHWIRAEIHEFVLKNWRLVKIATTKAQRKLFFNLRKAKQRLGWLSAEETR
                     AVADDLGVTPAEVNEMEQRLAARDMSLDPGPEADDEDRLTAPSYFLPAPGADPAELVA
                     DEDSDENAVTALEAALEDLDGRSRDILRRRWLGEEKATLQELADEYGVSAERIRQIEA
                     AAMKKLRGLIDRAA"
     gene            complement(4562..5572)
                     /gene="ftsX"
                     /locus_tag="PLS34_00325"
     CDS             complement(4562..5572)
                     /gene="ftsX"
                     /locus_tag="PLS34_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRP85947.1"
                     /translation="MGQGRRWRLRLPSLRWPAGGIAWPGGNPFGRVRSWLRLHAQTLV
                     GSLGRLAEQRLASLMTMAVIGIALALPASFLLLVTNLQSLSGHWDDSIELSLYLAPGE
                     AESRAVEMAEEIGSWPEVDGVQLITAAEALARFREGSGLGDAIDALDTNPLPNLVAVR
                     PADGHDDADSIEALAGRLRTLPGADVVQADADWVRRLFAMLQIARRLAGLAATLLAIG
                     VLVIIGNTIRLDIQNRRDEIEVVKLVGGTDGFIRRPLLYGGFWYGLGGGLAALLIVQL
                     GLLALTGPVERLAGLYGSGFRPLGLGAAGALALVSAGAALGWLGSWIAATRHLRRIEP
                     DL"
     gene            complement(5595..6281)
                     /gene="ftsE"
                     /locus_tag="PLS34_00330"
     CDS             complement(5595..6281)
                     /gene="ftsE"
                     /locus_tag="PLS34_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRP85948.1"
                     /translation="MIRFEQVHKRYPGGRDALRDLSFGIEAGEMAFLTGHSGAGKSTL
                     LKLISCIERPTRGQVLVGGRNTAGISRRQVPAFRRQIGMVFQDHKLLGDRTVFDNVAL
                     PLVIAGTGTRETGRRVRAALDQVGLLGKEALAPIMLSTGEQQRVGIARAIAGRPPLLI
                     ADEPTGNLDPELSLEIMYLFARLNRVGVTVLVASHDLALLERLGHRRIALGDGRLIED
                     RDHAFPSAPA"
     gene            complement(6286..7296)
                     /gene="ftsY"
                     /locus_tag="PLS34_00335"
     CDS             complement(6286..7296)
                     /gene="ftsY"
                     /locus_tag="PLS34_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRP85949.1"
                     /translation="MSRPDTDPSRQDAAPAPGAAPAEKRKGFFRRLRERINKGDSWLT
                     YDLAHLLPGGKVGEEFIEEMETRLLTGDVGVDATQQIVAGLEKKLWRDELGDSRAVLG
                     TIRQSMLEILAPCEAPLVVPPEVRPFVILVVGVNGAGKTTTIGKIAHMLKDQGLSVML
                     AAGDTFRAAAVEQLQAWGRRNEVPVISQGTGADPAAVAYDAFEAARARGIDVLLADTA
                     GRLHTQENLMDELRKVKRVLGRLDPNAPHEVLLVLDGGSGQNALLQARRFNEAVGLSG
                     VAITKLDGTAKGGIVLAIARELRVPLRFIGIGEGIEDLAPFEAGPFVDALLAERTPGA
                     AR"
     gene            complement(7412..9865)
                     /locus_tag="PLS34_00340"
     CDS             complement(7412..9865)
                     /locus_tag="PLS34_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85950.1"
                     /translation="MRSLYSVALAAAALALAGCLTDAKDPDAPSPPPTTGVPQIPVVG
                     DTFRAQFNPLSAIVPYPNDILGFVAAPGTDGTLNLPAQALQPLAPLVNQLDGFSTNAR
                     IQANFTGAVAPASLTPATVFLLEVAIDPSTKGVVGLSDATLCKLGAAPPAACAAIGVA
                     ATGNPFLVQGADYAVSVAADIDAGGQTVQLKPLRPLNSNRPNAFTPGTENGYLLFLTD
                     GIRDTGGTPAQPDTVFAQIRAGYISGAIQLPPPGAGLPPGLTTEQLLGIFIAAHLAVG
                     EVVGIAPANVVVTASFTTLDTTTVLETAAAMAEAGASAVERMVTPVPLPVAPEVVLPA
                     GSPVTTSLVLGLQGVNAGAIPGEGDVYAGAVQLPYYLATPSVEAPTAPLSQPWIAATE
                     SPLDGTSRVLNKYNPVPLQRSTVSVPVVITVPNDNTAYAAALGGTAVQPPQGWPVVIF
                     YHGITRNRLDMFALTEPLNDAGYAVIAIDQPLHGIAAAEIDFETITPEELGAILAANP
                     TALFRVPGVPERTFDLDLQNNTTGAAGPDGNIDASGTHFINLANGLVSRDNLRQAAVD
                     LVALKRTIPTIDIDGDDAPDFDGSRVHFIGQSLGGIAGTTFLSVEDSVATATLGVAGG
                     VVSDLLLDSFSFGPRIEAGLIGNGLVPNTSLYNNFIRDLQNIVDAGDPISHAARAAGN
                     SAIHFIQVDGDTVVPNSASNRLAAEMAKSGLTDFEFSAMPIIEPAGHKARVCFTTGAS
                     HGSQLDPGTSPESITATGEMQAQQLVFIAGNPLVPLPANGQTLIFGASGSPGAPAAYG
                     NFDEDCDAPDRTRTNAAPN"
     gene            10021..10638
                     /gene="rsmD"
                     /locus_tag="PLS34_00345"
     CDS             10021..10638
                     /gene="rsmD"
                     /locus_tag="PLS34_00345"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRP85951.1"
                     /translation="MARVGKRAGGTSKGGSAGRFRIIGGEWRGRRLALPQVADVRPTP
                     DRVRETVFNWLAPVIAGARCLDLYAGSGALGLEALSRGAAQVVFVDRAPAVTRQIAAH
                     LETLHCDRGEILCLDAARYLERPPVPFDIVFLDPPYRAGLLAPALAQLLAAGWTAPAA
                     SVYLEHESAVAPPALPQGWRLFRSAAAGQVRYHLLRPDPVPAEES"
     gene            10635..11132
                     /gene="coaD"
                     /locus_tag="PLS34_00350"
     CDS             10635..11132
                     /gene="coaD"
                     /locus_tag="PLS34_00350"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008489436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRP85952.1"
                     /translation="MSISALYPGTFDPITNGHQDLIRRAASIFRRVVVAIAANPAKAP
                     LFSLDERVELARAVLADIPNVEVVGYSGLTVQFAQEHGLKVMVRGLRAVSDFEFEFQL
                     ATMSRQIAGQVETVFLTPTEQFSFISSSMVREIALLGGDVSRFVDPVVETALKARAAA
                     GKADG"
     gene            11125..>11582
                     /locus_tag="PLS34_00355"
     CDS             11125..>11582
                     /locus_tag="PLS34_00355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP85953.1"
                     /translation="MVDRMVSRLRRVPAVLRIVLLACAWLPAAAWSVAPGQGAVASAH
                     PLATAAGMEVLAAGGNAFDAAVAVSAALGVVEPYSSGLGGGGFWLLHFAADGRQVLVD
                     GRESAPGAAHATMYLDAQGEPVPGLSLNGPLAAGIPGTPAALAHVSEHYGR"
     assembly_gap    11583..11600
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11601..12820
                     /locus_tag="PLS34_00360"
     CDS             <11601..12820
                     /locus_tag="PLS34_00360"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP85954.1"
                     /translation="APAIRHAREGYRVYDRMRLGLDFKAEQLRRWPGSEALLPGGEVP
                     PVGALVRLPGLAMVMERLAEDGAAGFYGGEIAARMVEAVRGNGGIWTLEDLAAYRVIE
                     RAPLVAEFEEGTRFVLPPPPSAGGVAIIQAMNLLQGYELERLDAVDRVHLVIEALRRA
                     FRDRGHLGDPDFVNMPLERLLSKDYAAGLASSIRLDRATPSEVFAPLHAADGGDHTTH
                     FSVIDAAGNRVAVTQTINGWYGSSFMVPGLDLLLNNEMDDFAVKPGEPNLYQLVGAGA
                     NNVAPGKRMLSSMTPTFIESPRGVAVLGTPGGSRIISMVLLASLAWMEGADAESMVAL
                     PRYHHQYLPDRVVHEADAFSTDVAAGLEARGHVLQESRRLYGNMNVVTWEYDGNRVEA
                     ATDPRGRVEGWTY"
     gene            12893..13522
                     /locus_tag="PLS34_00365"
     CDS             12893..13522
                     /locus_tag="PLS34_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85955.1"
                     /translation="MKTPAFALPGTIALVTLAVLAGVSPAVAADGAQHPADAFLERLA
                     LHCGQAFAGHIVANEPAQPDDPFDGKTLVMHVRECGPGEVKVPFHVGDDHSRTWVLTR
                     TDTGLRLKHDHRHEDGTDDAVTMYGGDTAAPGTASRQEFPVDQYSIDMFVREGLNASL
                     QNTWAMEIEPGKRFLYELARPSGRLFQVEFDLTAPVATPPTPWGHPEIE"
     gene            13570..15354
                     /locus_tag="PLS34_00370"
     CDS             13570..15354
                     /locus_tag="PLS34_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP85956.1"
                     /translation="MKKIATRFCRVLSVALLAVGLAAVANAGDWRFEGVERVVAIGDV
                     HGARDELVALLGDVGLVDAELRWSGGRTHLVSVGDLLDRGDYGRQVMDLLMRLQQEAA
                     AAGGAVHVLLGNHEVMNLTGDLRYVSEGDYAQFGTEARAGLPAGFFERRAALAPEGYY
                     GQWLLGLPVALVVNDTLFVHGGVSQMLEGMSLEQINRVALRDVRRVAEGWHALLGAGV
                     LGDTDGFSTLRSRAAALADDGRDAALQALGREMNEALQGLPFNPDGVLWYRGNSLCHA
                     YAEGETLRKVLAAVGARQVVVGHTVTGPRKITSRVDGLVYRIDTGMNHAAYGGTPGAL
                     IIEGGKPLAKYIGAPAAAAAVETTRDWARPYGMSDAELEDFLRTAEVTLVEDLAEGVT
                     RPQRLTLERDGRTMRAVFKTVDTNPGLERQRRWSREHDLADRHIYDVVAYRLDRILGV
                     DMVPVAVEREVNGRSGTAQYWVPNSINENQRRAQEIPRSSDCSFAPQFNIMNAFDLLI
                     FNTDRNLGNILYDEDQHIWLIDHTRAFGTRRGEPAMLRNAQVVITPDLAAMLEQVTEE
                     NLEPLKPYLHARQISALVTRAQWLRGRQ"
     gene            complement(15308..17029)
                     /locus_tag="PLS34_00375"
     CDS             complement(15308..17029)
                     /locus_tag="PLS34_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRP85957.1"
                     /translation="MNSSAPVISRLTIFERLLTLFTAVRPGEGRSIFVMLLQIFLLLF
                     GYYVIRLVRETLILVEGSPEIRAYSTGAIAVTLIFIIPLYKLLFDYLKNRGNKSAVLL
                     WVGGFFISNLLIFALLVWLGIPIAVPFYIWVGVFNVMVIAQFWAFAADLFNVKTGQRL
                     FAVIMMGAALGAMAGSQAGGRLFSMVGVTNLMLMSAALLAAVLLLSRHAERIVPEGSR
                     SVPAGPSAAPEGNLIGQVLGGFRVVARSGYLARIALFVMLLNLVNTNGGYVLVTFLSG
                     HARDVVAAGNGVLSTGDVIARFWGNYWTWFTALQIVIQLFFVARIFRWFGVRGAILVL
                     PAVMVLNYGLILFVPVFAVVRVMMIIENATNMSMGNTTNHTLYLPVTREEKYVGKTTI
                     DTFMARFGDLLSAGLVMLTAQFLGLEVGWMVAINLALALALFALGNAIGKYHRSQVRH
                     NLENLPPVVSRRLPDVYVPAGQVLMFSVPDRAFIDPDPGDALRYQARAADGGPLPGWI
                     RFDAHNQSFTIRPPAGRSGTLEVTLSATDFEGLSVDSGFGVSYGSDPIPRFIDAPATT
                     GPGSPAR"
     gene            complement(17043..17861)
                     /gene="mutM"
                     /locus_tag="PLS34_00380"
     CDS             complement(17043..17861)
                     /gene="mutM"
                     /locus_tag="PLS34_00380"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRP85958.1"
                     /translation="MPELPEVETTRRGVAPWIEGARVAAVELRQPMLRWPVPRQLVAE
                     LPGRRVLAAERRAKYLLFRAEGDRTLMLHLGMSGSLRVLQTYRPPEPHDHLDLVLETG
                     RVLRFHDPRRFGSADWVTGDPLRHPLLRELGPEPLGAAFTPDYLAAACRGRKVAIKQH
                     IMNARIVVGVGNIYASEALFRAGIHPARAAGRISPARLELLVEAVRDVLEAAIRQGGT
                     TLRDFAWGEGRRGYFRPELAAYDRAGEPCLRCGGIIRAVVQGQRSTYYCPSCQH"
     gene            17953..19152
                     /locus_tag="PLS34_00385"
     CDS             17953..19152
                     /locus_tag="PLS34_00385"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRP85959.1"
                     /translation="MTEFLLEHHRGLLGLSAWGYVLASLLMVQVTVFAVTLYLHRDAA
                     HRAVDLHPAIRHFCRFWLWLTTGIATREWVAIHRKHHARCETPDDPHSPQIEGLRKVL
                     LEGAELYRAEARNEETLRKYGRGTPDDWLERKVYGAARSYLGIVLMVALFLLLFGVPG
                     IIMVAVQLASQPLLAAGIVNGVGHYAGYRKFECADASRNVTPWGVLLGGEELHNNHHA
                     FPSSARFSIRPWELDIGWVVLRGLAAIGLADIRRTAPVPVRQPARQQLDLETVRAIIV
                     GRMHVMRDYTRLVTLPVLRAEFAALRARIPERAGRLRRLLVRETALLDQAARRRLHEV
                     LESNHALATVHEFRERLKEIWAGSATSNEGLIAQIRDWCAEAEASGVRALQDFAAGLR
                     GYSLRTA"
     gene            complement(19244..19399)
                     /gene="rpmG"
                     /locus_tag="PLS34_00390"
     CDS             complement(19244..19399)
                     /gene="rpmG"
                     /locus_tag="PLS34_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRP85960.1"
                     /translation="MRDKIKLVSSAGTGHFYTTTKNKRLHPEKMEVSKFDPKARKHVI
                     YKEAKIK"
     gene            complement(19411..19647)
                     /gene="rpmB"
                     /locus_tag="PLS34_00395"
     CDS             complement(19411..19647)
                     /gene="rpmB"
                     /locus_tag="PLS34_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRP85961.1"
                     /translation="MSRICQVTGKGPTTGNNVSHANNRTRRRFLPNLHTHRIWVESEQ
                     RFVRLRVSQQGLRIIDRKGIDTVLSELRARGEKV"
     gene            complement(19786..20469)
                     /gene="radC"
                     /locus_tag="PLS34_00400"
     CDS             complement(19786..20469)
                     /gene="radC"
                     /locus_tag="PLS34_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRP85962.1"
                     /translation="MPHLPIRDWPMAERPREKLLAGGPDRLSDAELLAIFLRTGTPGR
                     SAVDLARELLLGFGGLRGLLTADLASFVARPGLGPVKYVQLQAGLELARRHLEETVRR
                     GPALGSPDAVRRFLRARLRDLPHEVFCCLHLDNRNRVIHFEELFRGTIDGASVHPREV
                     VKRALARNAAALILAHNHPSGVAEPSQADEVITRRLKSALALVDIRVLDHLVVGDGAC
                     VSFAERGLL"
     gene            20550..21773
                     /gene="coaBC"
                     /locus_tag="PLS34_00405"
     CDS             20550..21773
                     /gene="coaBC"
                     /locus_tag="PLS34_00405"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRP85963.1"
                     /translation="MPTLAGKQVLLGVTGGIAAYKSPDLVRRLREQGAEVRVVLTPAA
                     ERFVTPLTFQAVSGHPVRGGLWDESAEAAMSHIELARWADLVLVAPATADFLGRLAAG
                     LADDLLATICLATEAPIVVAPAMNRLMWANPATQDNVRRLAARGVTVLGPGSGNQACG
                     ETGEGRMLEPAEIAAGLPVAAGRRVGVLAGRRVLVTAGPTREALDPVRYLTNRSSGRM
                     GYAVAAAAARAGAEVVLVSGPVDLAPPAGVRVVPVDTAAAMRAAVQAQLPGTAVFVAA
                     AAVADYRPAAPAPQKIKKGAAEMTLALLRNPDILAEVAQASPRPFVVGFAAETENVEG
                     NARGKLEGKSLDMIAANRVGSDCGFDREDNALLVLWPGGGREDLGSGSKAELADRLVR
                     LIAERMDAGHQAQGS"
     gene            21745..22200
                     /gene="dut"
                     /locus_tag="PLS34_00410"
     CDS             21745..22200
                     /gene="dut"
                     /locus_tag="PLS34_00410"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRP85964.1"
                     /translation="MQVIKLKVLDSRLGAEFPLPEYATPGSAGLDLRACLDAPLVLQP
                     GQTELIPTGLAIHIDDPGLAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSAWN
                     RGAAEFTVQPGERIAQMVVVPVVRARFEIVADFSASERGAGGFGHSGRH"
     gene            complement(22217..22819)
                     /locus_tag="PLS34_00415"
     CDS             complement(22217..22819)
                     /locus_tag="PLS34_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRP85965.1"
                     /translation="MKTRDFWLAAILATACAATAVPAAAAGKVYRWVDEHGVVHYGDA
                     IPPEYSKERHEVLDGRGTRVTVHGEKPEPDAPARDNRDRALLATYGSVAEIESVRDRR
                     IGYLDDQNEVALDRLQSLRARREALADNPAAINELATVEQRIREYVYEISRRNAEIER
                     IRDQFQGDIRRFEELRGAEGSAEDQSEESAARAEATPPER"
     gene            complement(22896..23543)
                     /gene="pyrE"
                     /locus_tag="PLS34_00420"
     CDS             complement(22896..23543)
                     /gene="pyrE"
                     /locus_tag="PLS34_00420"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRP85966.1"
                     /translation="MHSYKHAFIELALARAALKFGSFTLKSGRESPYFFNAGLFNTGA
                     ALAELGACYADAIVAAGLQFDVLFGPAYKGIPLATATAMALAGRHGRDLPWVFNRKEA
                     KDHGEGGNLVGAPLAGRVLIVDDVITAGTAIRESIGIIRAAGAEPCGVLLALDRQERG
                     RGERSAVQEVEQDLGLPVTSIISLADLLAHLEHGGGDHPERLAALQEYRRRYGVA"
     gene            23637..24416
                     /locus_tag="PLS34_00425"
     CDS             23637..24416
                     /locus_tag="PLS34_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRP85967.1"
                     /translation="MRIISLNANGIRSAARKGFFEWLAGQDADVVCLQETRAHEHQIS
                     DDAFRPAGYHCHYLAAERKGYSGVALYSKRAPERVVRGFGSAEFDPEGRYIEAVFPGL
                     SVASLYMPSGSSSEERQAAKYRFLDEFEDFLRRVRRRPREYVICGDWNIAHKEIDIKN
                     WRGNLKNSGFLPEERAWMDQLFGRWGFVDAFRAVDPRPERYTWWSNRGRAREKNVGWR
                     IDYQVVTPGLADRIRGANIYTGHRLSDHAPLILDYDFDLPT"
     gene            24461..25774
                     /locus_tag="PLS34_00430"
     CDS             24461..25774
                     /locus_tag="PLS34_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP85968.1"
                     /translation="MATLLALGFSSGLPAPLVFTNLSIWLRDDGVSRTDIGLFALAAT
                     PYAINFLWAPLVDRMRLPFFSARFGRRRGWALFTQLSLVAAIVVLSRAQPAADLFAVA
                     AAVLFVTFVSATQDVVIDAYRIDVLSPEQYGAGSAAAIWGWHLGGTLIGGAGGLYLAQ
                     VFGWNTAYLFLAAGVGVGMLAILLSPEPPFRPPADTLEHEARVAELLRRVSWLRGRLA
                     GAAAWLYGAVVAPFVDFMKREGWLLVLAFIIVFKFGDALLGRMSGVFYRELGFSLVEI
                     AEVMKVYGFIANGIGVLAGGVLVARIGLFRALLIAGLLAAATNLSYSWLAMSGNDPVV
                     FRIAVITDNFTGGLATVAFVAYLSSLCNVAYTATQFALLASAGNLGRIWLSASSGWMV
                     DALDGDWAVFFAMTAGIALLGLPLLLVLMRRYPETQARAPEPERD"
     gene            25885..26517
                     /locus_tag="PLS34_00435"
     CDS             25885..26517
                     /locus_tag="PLS34_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85969.1"
                     /translation="MAMNLDDLDQLAARAARYARYSRSAGGLSSVIGGLLLLASFAAN
                     AFAELAFWQRIALALTPALWLLAKELLRARYYQRDGRALQAPTARERRNHRLIVAYLA
                     VISVIVLGVFVHAALEAGRLPGPGMLGYAVIVAVLPVVAWRWFWSVSDFLVGVLLMCQ
                     SAVVIGGGHYPAIWLLYVGACAAIAVLYGLREHREYLALRAEFTARTPLQ"
     gene            26514..26858
                     /locus_tag="PLS34_00440"
     CDS             26514..26858
                     /locus_tag="PLS34_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRP85970.1"
                     /translation="MKDSTRSAIDGVLNVDRLVHEPARLAILTVLSQAEFAEFGFLEM
                     ACRLSKGNLSSHLSKLEAGGLVTIEKRFEGKRPQTRAAITESGRAALANYREHLKALI
                     SEVPTGAEGDQQ"
     gene            26855..28315
                     /locus_tag="PLS34_00445"
     CDS             26855..28315
                     /locus_tag="PLS34_00445"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRP85971.1"
                     /translation="MKRLTGRLAITFLWCMSLHANTAAAADEVAARETALADGRALTA
                     QFLAGEADALWPRFGAQLRGVIGSPAGLRGFSTQIREQLGAEDEVLRETVQRQGEFTV
                     YQRIGRWSLAPMPIAVTWAFGPDGRVEGFQVAPAPAEAPAADPARAPVESGRLPDDAT
                     VAAQLQAFVEQAGAAPGVVVGLHDAQGTRFQAWGDAGDGAPPDADTVFEAGSITKGLT
                     GLLLAQMVAAGEVELEQPIGDLLPDGLELAPGLAAITLGELATHRSGLPRLASGPEMQ
                     ARMTSDDPYAGSTTVEIFADLARVMPETVAAGRGRFAYSNLGTAVLGQLLARSVGQPY
                     EDLLAARVFQALDLPGPVLDPDAVTGRRAVGTQAGAPVKAWRFDAYAPMGAWQASAHE
                     LVALGRRLLQTEPDWVGAALAPQAVPGHPGTGMGLGWHHAELGARRFVWHNGGTAGCS
                     SFLAVVPAEGLVVAVLANGGGGIADGLARELLVSGR"
     gene            complement(28364..28774)
                     /locus_tag="PLS34_00450"
     CDS             complement(28364..28774)
                     /locus_tag="PLS34_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRP85972.1"
                     /translation="MPTGSYASPEACEAAFYDAFRDGDLASMQHVWGMDGEVTCIHPG
                     RPPLAGRRAVLQSWEEILGATGGVQVRFDCQSRVRGDDLAVHVGIEVIGQPDSDAALV
                     TVTNVYTLTEQGWKLRTHHGGPIHRGASARGPVH"
     gene            complement(28778..29071)
                     /locus_tag="PLS34_00455"
     CDS             complement(28778..29071)
                     /locus_tag="PLS34_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85973.1"
                     /translation="MPLPIPAIGDWYQAPTGVRFEVIAVDDEDGSIDIQHFDGTVEEI
                     EFDAWEESEFLPADPPEDYSGSLDIERDDYGMDLDRAAVRDWSDPLDFLDQAE"
     gene            29212..29832
                     /locus_tag="PLS34_00460"
     CDS             29212..29832
                     /locus_tag="PLS34_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRP85974.1"
                     /translation="MTEQLIKTFIIFFVVIEPVSLVPMFGALTRGGEPGYRTRMAWKS
                     VAISAGIFIVFALAGDWLLRLLGISVSAFKIAGGVLLFLLAVDMVLARESGLRSTTVR
                     EQEEARYREDISVFPLAFPLITGPGALATLLLLTADTANYIEFSLVLGMGLLALAVTL
                     AFLLLTQPLMRVMGVTGGTVVSRLLGVVLAALAVQYMVDGVTEVLA"
     gene            complement(29918..>30075)
                     /locus_tag="PLS34_00465"
     CDS             complement(29918..>30075)
                     /locus_tag="PLS34_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRP85975.1"
                     /translation="MLEVCEASPHFENAAPGRGYADRSETRPVTRFERRGLKLGHQVF
                     DLLYRRQ"
BASE COUNT         4419 a        10222 c        10716 g         4700 t
ORIGIN      
        1 ggccgagctg caacgacagg agctgggccg tggtgatgcg ccgcgccatg tcggccagcc
       61 ggcgctgcac cgtctgggtg tgggacaccg gccggttgaa cagctcgcgg tcgttcgagt
      121 agtcgagcgc ctctttcagg caggcctgcg cggcgccgat ggcgccccag gtgatgccgt
      181 aacgcgcctg ggtcaggcag ccgagcgggc ccttcaggcc gaccacgccg ggcagcatgt
      241 tctctgcagg cacgcgcaca ttgtcgagga acagcgacga ggtcaccgag gcgcgcagcg
      301 agaacttgcg gtgaatctcg ggcgcgtcga atcccttcgt gcccttttcc acgaggaagc
      361 cgcgcaggcc ctcgtcggta atcgcccaca cgaccgcgac gtcggcgacg ctgccgttgg
      421 tgatccacat cttggcgccg ttgatgaccc agtcgccgcc gtcgcgtttc gcgtgggtct
      481 tcatgttgcc ggggtcggag ccgccgtgcg gctcggtgag gccgaagcag ccgatggcct
      541 cgccggcggc cagcgcgggc agccagcgct gcttctgcgc ctcgctgccg taggtgtaga
      601 tcggccacat gaccaggctc gactgcaccg agacgaagct gcggatgccc gagtcgccgc
      661 gctccagctc ctggcagatg aggccgtagg acacgccgtt caggccggcg cagccgtagc
      721 cctcgagcga ggagcccagc agccccagcg cagcaatttc cggcaccagc tcggccggaa
      781 agcggtgctc ctcgaagcac tcgcggatga tgggcagcac cttttcgtcg gtgaagcgcg
      841 cgacggtgtc ctgcaccatc tgctcgtcct cgctcagtgc accgcggacg tcatagaaat
      901 cgaggggatc gacggaactc acgctggact ctcccggctg gcctgctccg ggcggcggcg
      961 cccggggaaa gcgccattct agccgaactg cctgctgcgg ccgcggcggc tttgcgtcca
     1021 taatgttgcg catgaagatt cccgagatcc tgctgattga gtccgagcac cgtcccggca
     1081 gcctggccag catcctcgcg gtcatcggcg agcaagggct gctggtggag cacctggcgg
     1141 cgctgcgccg ggagcaggac aagacggtct gggaaatcac cctcgagatg gacccggccg
     1201 ccgggcggcg gctcgaggaa cgcatcaacg cgctgccgaa cgcccgcgtg ctggggcgct
     1261 ccgaccgggt cttcaaccgg catcgcggcg gcaagatccg caccgtcagc acgctggaca
     1321 tcggcagcct gcagatcctg cgcgacgtct acacgccggg ggtcgcgcgc gtctgcctgg
     1381 cgatccgcga tcagccggag ctggcgcgcg agttcaccgc catcggccgc acggtcgcca
     1441 tcgtcaccaa cggcacggcg gtgctcggcc tgggcgacat cggcgcgctg gccggcatgc
     1501 cggtgatgga gggcaaggcg gcgctgatgg cgagcctggc gggcctgtcg gcggtgccga
     1561 tcctggtcga ggagaaggac ccggcgcggc tgaccggcgt catcgccgcc atcgccccgt
     1621 cgttcggcgc gatccagctc gaggacatcg cggcgcccgc atgcttcgag gtcgaggacg
     1681 cgctgcgcgc gcagctcgac attccggtgc tgcacgacga ccagcacggc acggccgtgg
     1741 tggcgctggc ggcggtgcgt aacgcgggcc gccaggccgg ccagcggctg tcgcagagca
     1801 cggtggggca ggtcgggctg ggcgcggccg gcatcggcat cgcccggctg ctgctgtcgc
     1861 gcggcacgaa gcgcatcctc ggcgccgacc tgaaccctgc ggcccgcgac cgtttcgcgg
     1921 cgctcggggg tgagcccgcc gagctggcgc gggtgatggc ggaagcggac atcgtcatcg
     1981 cgaccacggg cgtgaaaggc ctgatccgcc ccgagtcggt gcggccgggc caggtcatcc
     2041 tggccttgtc gaatcccgac ccggagatcg agccgcaggc cgcgctcgcc gccggggccg
     2101 cattcgccgc ggacggcaag agcatcaaca acgtgctggg attcccgggg ctgttccggg
     2161 gggcgctcga cgccggcgcg cggcgcttca ccgacgagat gctggtggcg gcggccgagt
     2221 cgatcgcgga ccttgccggg gagcacgacc tggtgcccga cccgctcaac aaggccgtcc
     2281 acgacgccgt ggcgcgtgcc gtagcgggcg ccgccgcatc gtccggcgcg tgccaggccg
     2341 ccggggtggc tcagtaaggg atgccggcgc gccggtaggc cgcggccagc agccacagcg
     2401 ggccgatcat caggaactgc aggtccttga agaaggacgg cttgcggccc tcgatccggt
     2461 gcccgatgaa ctgcccgacc caggccgcga cgaacacggc cagcgagacg gcccacaggg
     2521 gcgctgcagc gctctccagc accgccgcga gccacagcag gacggccagc acgagggtgg
     2581 cgccgaagcc cagcggcagg gacaggatga agtaatagac caccgacgcc atcgagaaca
     2641 gcagcgccca gttcagcgcc ggggagatgt ccgcgaaggc cccggggacg ggcagcgacc
     2701 acagcaggcc gatcacgctg aacacgatcg ccggcacgca gatccagtga atcagcttgt
     2761 tggcgggatg ctggtgactc tcgccgtatt catcgagcca ggactctagg tcgtgcaagg
     2821 gcgtgctccc gtcgccgggg gcgccgcgcc ggtgccccgc tacggcgcag aaggtatcat
     2881 gacgcccaat gctgtgcttt cggcagctca tcagagggac atttcatgaa caagaccact
     2941 gcacgtatcg tgatgctggc cgcggcagcg gcgatgacgg tcggctgcca gaccatcgat
     3001 ccgtacacgg gcgagacgaa gacttcgagc gccaccaagg gcgcagccat cggtgcgggt
     3061 gcgggcgccg tgctgggcat tctcaccggc gatgactcca aggagcgccg caagcgcgcg
     3121 ttgatcggcg ccggcgtcgg cgcgatcgcc ggcggcgcgg tggggtccta catggacaac
     3181 caggaagcca agctgcgcga gcagatggag ggcaccggcg tcagcgtcac gcgcgtcggc
     3241 aacgacatca tcctgaacat gccgggcaac gtcactttcc agaccaacag ctcggacctg
     3301 aacccgcaat tcttcggcgt gctcgactcc gtgggactgg tgctcaagga gtacgacaag
     3361 accgtcatcg aggtagtcgg ccacaccgac agcacgggct cggccgagta caaccaggcg
     3421 ctttccgagc ggcgcgcggc cacggtcgcg acctacatca acaaccgcgg catcgaccgc
     3481 cagcggattc tcgccttcgg gcgcgggctc acccagccga tcgccgacaa ttcgacggcg
     3541 gcggggcgcg ccatgaaccg ccgggtggag ctgacgctct ctccgatcac caactagtcg
     3601 aacaggatcg gatcaggctg cgcggtcgat caggccgcgc agctttttca tggccgcggc
     3661 ctcgatctgc cggatccgct cggcggacac gccgtattcg tcggccagct cctgcagggt
     3721 ggctttttcc tcgccgagcc agcgccggcg caggatgtcg cggctgcgcc cgtcgagatc
     3781 ctcgagggct gcttcgagcg ccgtgacggc gttctcgtcg gaatcctcgt cggcgaccag
     3841 ctccgccggg tcggcgccgg gcgcgggcag gaaatacgaa ggcgcagtga ggcggtcctc
     3901 gtcgtcggcc tccgggccgg ggtccagcga catgtcgcgc gccgccagcc tttgctccat
     3961 ctcgttgact tcggccggcg tcacgcccag gtcgtcggcg accgcgcggg tttcctcggc
     4021 cgagagccag ccgagccgct gcttggcctt gcgcaggttg aagaacagct tgcgctgcgc
     4081 cttggtggtc gcgattttta ccagacgcca gttcttgagc acgaactcgt ggatctcggc
     4141 gcggatccag tgcacggcga atgacaccag gcgcacgccg acggaggggt cgaaacgctt
     4201 gacggccttc atcaggccga tgttgccttc ctgaatgaga tcgccgagct gcaggccgta
     4261 gccgttgtag ccgcgcgcga tgtgcaccac gaagcgcagg tgggacagca ccagctgccg
     4321 cgccgcatcc aggtcgttgt cgcgacggaa gcgccacgcc agctcgctct cctcctcggc
     4381 gctgagcacc gggatgctgg cgacggcgtt gatgtacgcg ccgaggttgc cgaccggccc
     4441 ggtcagcatc agctcgttct ggcgtgcgac gagcgcgttg ctcattcaat tcctcccttc
     4501 gcggccggat tttagcactc gaacggttag agtgctaagc ctgccccggg ttcccggtcg
     4561 gctagaggtc gggctcgatc cggcggaggt ggcgggtggc ggcgatccag gagccgagcc
     4621 agccgagcgc cgcgcccgcg gacaccaggg ccagggcgcc ggccgccccg agcccgagcg
     4681 gccggaatcc ggagccgtac aggccggcga gccgctccac cgggccggtc agcgccagca
     4741 gcccgagctg cacgatcagc agcgcagcca gcccgccgcc gagcccgtac cagaagccgc
     4801 cgtacagcag gggccggcga atgaacccgt cggtgccgcc gaccagcttc accacctcga
     4861 tctcgtcgcg ccggttctgg atgtcgagcc ggatagtgtt gccgatgatc accagcacgc
     4921 cgatggcgag caacgtggcg gcgagcccgg ccagccgccg ggcgatctgc agcatggcga
     4981 acaggcgacg cacccagtcc gcgtcggcct gcacgacgtc cgcccccggc agggtgcgga
     5041 gccgcccggc gagcgcctcg atgctgtctg cgtcgtcatg cccgtccgcc ggacgcaccg
     5101 cgaccaggtt gggtagcgga ttggtgtcca gcgcatcgat ggcgtcgccc agccccgagc
     5161 cctcgcgaaa gcgggccagc gcctccgcgg cggtgatgag ctgcactccg tccacctccg
     5221 gccagctccc gatctcctcc gccatttcga ccgcgcggga ctcggcctca cccggtgcga
     5281 ggtacagcga aagctcgatg ctgtcgtccc agtgtccgga caggctctgg agattggtca
     5341 ccagcagcag gaagctggcg ggcagcgcca gcgcaatgcc gatcacggcc atggtcatga
     5401 ggctcgccag gcgctgctcg gccagtcggc ccagcgagcc caccagcgtc tgggcgtgca
     5461 gccgcagcca gctgcgcacc cggccgaacg ggtttccgcc gggccatgcg atgccgccgg
     5521 ccggccagcg cagcgacggc agccgcaatc gccagcggcg accctggccc acgcggccct
     5581 tgcgcctgcg gggcctaggc gggcgcactg gggaacgcat ggtcgcgatc ctcgatcagc
     5641 cgcccgtcac cgagcgcgat gcgccggtga ccgagccgct ccagcagggc caggtcgtga
     5701 ctggccacca gcaccgtgac gccgacgcga ttgaggcggg cgaacaggta catgatctcc
     5761 agcgacagct ccgggtccag gttgccggtg ggctcgtcgg cgatcagcag gggcggccgg
     5821 ccggcgatgg cgcgagcgat gccgacccgc tgctgttcgc cggtggacag catgatcggc
     5881 gccagcgcct ctttgcccag gaggcccacc tggtcgagag ctgcgcgtac gcgccggccg
     5941 gtttcgcggg tgccggtgcc ggcgatcacc agcggcaagg cgacgttgtc gaacaccgtg
     6001 cggtcgccga gcagcttgtg gtcctggaaa accatgccga tctggcgccg gaatgccggc
     6061 acctggcggc gcgagatgcc ggcggtgttg cggccgccga ccagaacctg tccccgggtg
     6121 ggccgctcga tgcaggaaat gagcttcagc agcgtgctct tgccggcccc ggaatgcccg
     6181 gtcaggaacg ccatctcgcc ggcctcgatg ccgaaactga ggtcgcgcag ggcgtcccgt
     6241 ccgccgggat agcgcttgtg gacctgctcg aagcggatca cggcgtcacc tcgccgcgcc
     6301 gggcgtccgc tcggccagca gggcgtcgac gaagggtccg gcctcgaagg gcgcgagatc
     6361 ctcgatgccc tcgccgatcc cgatgaagcg caacggcacg cgcagttcgc gcgcgatggc
     6421 cagcacgatg ccgcccttcg cggtaccgtc cagcttggtg atcgcgacac cgctgaggcc
     6481 gaccgcctcg ttgaagcgcc gtgcctgcag cagcgcgttc tggccgctgc cgccgtccag
     6541 caccagcagc acctcgtggg gcgcattcgg gtcgagccgg ccgagcacac gcttcacctt
     6601 gcgcagctcg tccatgaggt tttcctgggt gtgcagccgg cccgcagtgt cggccagcag
     6661 tacgtcgatg ccgcgcgcgc gggcggcctc gaaggcgtcg taggcgacgg cggccgggtc
     6721 cgcacccgtg ccctggctga tcaccgggac ctcgttgcgc cggccccagg cctgcagctg
     6781 ctcgaccgcc gcggcgcgga aggtatcgcc ggcagccagc atgaccgaca ggccctggtc
     6841 cttgagcatg tgcgcgatct tgccgatggt ggtggtcttg cccgcgccgt tgacgccgac
     6901 caccaggatg acgaaggggc gcacctcggg agggacgacc agcggcgctt cgcagggggc
     6961 aaggatctcc agcatcgact ggcgaatggt gccgagcacc gcgcggctgt cgccgagctc
     7021 gtcgcgccag agcttcttct ccagccccgc gacgatctgc tgcgtcgcgt ccacgccgac
     7081 gtcaccggtc agcagccggg tttccatctc ctcgatgaat tcctcgccga ccttgccgcc
     7141 gggcaacagg tgtgcgaggt cgtaggtcag ccagctgtcg cccttgttga tgcgctcgcg
     7201 caggcgccgg aagaagccct tgcgcttttc cgcgggcgct gcgccggggg cgggcgcggc
     7261 gtcctgccgg gaaggatcgg tatcgggtcg ggacatggtt gaccggggtg atgtcgggcg
     7321 agccgttatg ctaccgcctt cgccgcagtg ccggaaagaa agcgggcggc ccgagggccg
     7381 cccgcaagtg aggcgagact gtcgccgaag gctagttcgg cgcggcgttg gtccgcgtgc
     7441 gatctggcgc gtcgcagtct tcgtcaaaat tgccgtaggc cgccggcgcg ccagggcttc
     7501 cgctggcacc gaaaatcagc gtctggccgt tggccggcag gggcaccagc gggttgccgg
     7561 cgatgaacac cagctgctgg gcctgcatct caccggtggc ggtgatcgat tccgggctcg
     7621 tgcccgggtc gagctgcgag ccgtggctgg caccggtggt gaagcagaca cgcgccttgt
     7681 gaccggccgg ctcgatgatc ggcatggccg agaactcgaa gtcggtaagc cccgacttcg
     7741 ccatctccgc ggccaggcgg ttcgacgcgc tgttcggcac cacggtgtcg ccgtccacct
     7801 ggatgaagtg gatggcgctg ttgccggccg cccgcgccgc gtggctgatc gggtcgcccg
     7861 catcaacgat gttctgcaga tcgcggatga agttgttgta caggctggtg ttcggcacca
     7921 ggccattacc gatcaggccg gcctcgatcc gcggaccgaa ggagaagctg tcgagcagca
     7981 ggtccgacac cacgccgccg gcgacgccca gggtcgccgt cgcgacgctg tcctccaccg
     8041 acaggaaggt cgtgccggcg atgccgccga gcgactggcc gatgaagtgc acccggctgc
     8101 cgtcgaagtc cggcgcatcg tcaccgtcga tgtcgatggt cgggatcgtg cgcttcagcg
     8161 cgacgaggtc gacggcggcc tggcgcaggt tgtcgcggct caccagcccg ttggccaggt
     8221 tgatgaagtg cgtgccgctg gcatcgatgt tgccgtccgg accggccgca ccggtggtgt
     8281 tgttctgcag gtccaggtcg aaagtgcgct ccggcacgcc cggcacgcgg aacagcgccg
     8341 tcgggttcgc cgcgaggatc gcgccgagct cttcgggcgt gatggtctcg aaatcgatct
     8401 ctgcggcggc gatgccgtgc agcggctggt cgatcgcgat gaccgcgtag cctgcgtcgt
     8461 tgaggggctc ggtgagcgcg aacatgtcga gccggttgcg ggtgatgccg tggtagaaga
     8521 tcaccaccgg ccagccctgc ggcggctgga cggcggtgcc gccgagcgcg gccgcatagg
     8581 cggtgttgtc gttcggcacc gtgattacga ccggcacgct gacggtggag cgctgcaacg
     8641 gcaccgggtt gtacttgttc agcacgcggc tggtgccgtc gagcgggctc tcggtcgccg
     8701 cgatccacgg ttgcgacagc ggcgccgtgg gcgcctcgac ggacggcgtg gccaggtagt
     8761 agggcagctg gaccgcgccg gcatagacgt cgccttcgcc ggggatggcg cctgcgttca
     8821 cgccctgcag gccgagcacc aggctggtgg tcaccggcga gcccgccggc agcacgactt
     8881 ccggcgccac cggcaatggc accggcgtca ccatccgttc gaccgccgac gcaccggcct
     8941 cggccatcgc tgcggcggtc tccagcaccg tggtggtgtc gagcgtggtg aagctggcgg
     9001 tcacgacgac gttggccggc gcgatgccga ccacctcgcc gaccgcgagg tgggcggcga
     9061 tgaagatgcc cagcagctgc tcggtcgtca ggcccggcgg caggccggcg cccggcggcg
     9121 gcagctggat cgcgccggaa atatagccgg cgcgaatctg cgcgaacacc gtgtccggct
     9181 gggccggggt gccgcccgtg tccctgatcc cgtcggtcag gaacaacagg tagccgtttt
     9241 cggtgcccgg ggtgaacgcg ttggggcggt tggagttcag cggccgcagc ggcttgagct
     9301 ggacggtctg gccaccggcg tcgatgtcgg cagcgacgct gaccgcatag tctgcgccct
     9361 gcaccaggaa gggattgccg gtcgcggcca cgccgattgc ggcgcaggcc gcgggcggtg
     9421 ccgcgccgag cttgcacagc gtcgcgtcgg aaagccccac cacgcccttg gtcgacgggt
     9481 cgatcgccac ttcgagcagg aacaccgtgg ccggcgtcag gctcgcgggg gcgacggcgc
     9541 cggtgaaatt ggcctggatg cgggcgttgg tggagaagcc gtccagctgg ttgaccagcg
     9601 gcgccagcgg ctgcagtgcc tgggcgggga ggttcagggt cccgtcagtg cccggagcgg
     9661 ccacgaagcc gaggatgtcg ttcgggtagg gcacgatcgc gctgagcggg ttgaactggg
     9721 cgcggaacgt gtcgcccacg accggaatct gcggtacgcc ggtagtgggg ggcggtgacg
     9781 gcgcatcagg atcctttgcg tccgtcaggc acccggcaag cgccagggcg gcggctgcaa
     9841 gcgccaccga gtagaggctt ctcattgttc tgactcctcc caacaggggt gatcttttat
     9901 cgaatcgtta tggtcagcga cgcccacggc gcgactcggc catcaatggc tgccaccgcg
     9961 catgcgtccc gctagcctac gctggttgtc gctgccgttt caaggcctta actttgctgc
    10021 atggcgagag tcggcaagcg cgctggaggc acttcaaaag gcgggtccgc aggccgcttc
    10081 aggatcatcg gcggcgagtg gcgcggccgc cggctggcgc tgccgcaggt cgccgacgtg
    10141 cggccgacgc cggaccgggt tcgggagacg gtgttcaact ggctggcccc ggtcatcgcg
    10201 ggggcgcgct gcctggacct ctatgccggc agcggcgcgc tcgggctgga agcgctgtcg
    10261 cgcggcgcgg cacaggtcgt gttcgtggat cgtgcacccg cggtcacccg ccagatcgcc
    10321 gcgcacctcg agacattgca ttgcgaccgc ggcgaaatcc tctgcctgga tgcggcccgc
    10381 tacctggaac ggccgccggt gccgttcgac atcgtgtttc tcgatccgcc ctaccgcgcc
    10441 ggcctgctcg cgccggcgct ggcgcagttg ctcgccgccg gctggaccgc gcccgcggcc
    10501 agcgtctacc tggagcacga aagcgcagtc gccccgcccg ccctgccgca aggctggcgc
    10561 ctgtttcgca gcgccgccgc ggggcaggta cgctaccatc tgcttcgccc cgatcccgta
    10621 cccgcagagg aatcatgagc atctcggcgc tgtatcccgg cactttcgac cccatcacca
    10681 acggccacca ggacctcatc cggcgcgccg cgtcgatctt ccggcgcgtc gtcgtcgcga
    10741 tcgccgcgaa cccggccaag gcaccgctgt tcagtctcga cgagcgagtg gaactggccc
    10801 gcgcggtgct ggccgatatc ccgaacgtcg aagtcgtcgg ctacagcggc ctgacggtgc
    10861 agttcgcgca ggagcacggc ctcaaggtga tggtgcgcgg cctgcgggcg gtgtcggatt
    10921 tcgagttcga gttccagctg gcgaccatga gccggcagat cgccggccag gtggagaccg
    10981 tgttcctcac gccgaccgag cagttcagct tcatctcgtc gagcatggtg cgcgagatcg
    11041 ccctgctcgg cggcgacgtg tcccgtttcg tcgacccggt ggtggaaacc gcgttgaagg
    11101 cgcgtgcggc ggcaggcaag gccgatggtt gatcgcatgg tgtccaggct gcgacgggta
    11161 cccgccgtgc tgcgcatcgt gctgctggct tgcgcctggc tgccggctgc cgcctggtcg
    11221 gttgcgccgg gccagggcgc ggtggcgagc gcgcacccgc tggcgacggc ggcgggcatg
    11281 gaggtcctcg cggccggcgg caacgccttc gatgccgccg tggcggtgag cgcggcgctc
    11341 ggggtggtcg agccctattc ctcaggtctc ggcggcggcg gcttctggct gctgcatttc
    11401 gccgcggacg gccgccaggt gctggtggac ggccgggagt ccgcgccggg cgcggcgcac
    11461 gccaccatgt acctcgatgc gcagggcgaa ccggtccccg gcctgtcgct gaacggcccg
    11521 ctggcggccg gcatccccgg gacaccggcc gcgctggcgc acgtcagcga gcactacggc
    11581 cgnnnnnnnn nnnnnnnnnn tggcgccggc catccgccat gcgcgcgagg gttaccgggt
    11641 gtacgaccgc atgcgtctcg ggctggactt caaggccgag cagctgcgcc gctggcccgg
    11701 tagcgaggca ctgctgccgg gcggcgaggt gccgccggtc ggcgccctgg tccggctgcc
    11761 cgggctggca atggtcatgg agcggctggc ggaggacggc gccgcgggct tctacggcgg
    11821 cgagatcgcc gcccggatgg tcgaagccgt gcggggcaac ggcggcatct ggacgctgga
    11881 ggacctggcg gcctaccgcg tgatcgagcg cgctccgctg gtcgcggagt tcgaggaggg
    11941 gacgcgtttc gtgctgccgc cgccgccgtc ggcgggcggc gtggccatca tccaggcgat
    12001 gaacctgctg cagggctatg agctggagcg cctggatgcg gtggaccgtg tgcacctggt
    12061 catcgaggcg ctgcggcgcg cctttcgcga tcgcggccat ctcggcgacc ctgatttcgt
    12121 gaacatgccg ctggagcgcc tgctgagcaa ggactacgca gccggcctgg cgagctcgat
    12181 ccgcctggac cgcgccaccc ccagcgaggt gttcgcaccg ctgcacgcgg ccgacggcgg
    12241 cgatcacacc acgcatttct cggtcatcga cgccgcgggc aatcgcgtcg ccgtgaccca
    12301 gaccatcaac ggctggtacg gcagcagctt catggtgccc gggctggacc tgctgctgaa
    12361 caacgaaatg gacgatttcg ccgtcaagcc cggcgagccc aacctctacc agctggtcgg
    12421 cgcaggcgcc aacaacgtcg cgccgggcaa gcgcatgctg tcgagcatga cgcccacgtt
    12481 catcgagtcg ccgcgcggcg tcgcggtgct cggcacgccg ggcggcagcc gcatcatcag
    12541 catggtcctg ctggcctcgc tggcgtggat ggaaggcgcg gatgccgaat ccatggtcgc
    12601 cctgccgcgc taccaccacc agtacctgcc cgaccgggtg gtgcacgagg ccgacgcctt
    12661 cagcacggac gtcgccgccg ggctcgaggc ccgcggccac gtcctgcagg aaagccggcg
    12721 gctgtacggc aacatgaacg tggtgacctg ggaatacgac ggcaatcgtg tcgaggcggc
    12781 gaccgacccg cgcggccggg tcgagggctg gacctactga gtcgggtcgg cccttgtcgg
    12841 gaccccgcaa tctgcttgcg gcgccgggtg gcggcgcgct agggtggcgt gcatgaaaac
    12901 acctgcattt gccctcccgg gcacgatcgc cctcgtcacg ctggccgttc tggccggcgt
    12961 ctctcccgcg gtggccgccg acggcgcgca acatccggcc gacgccttcc tcgagcgact
    13021 tgcgttgcac tgcggccagg ccttcgccgg ccacatcgtc gccaacgagc cggcgcagcc
    13081 ggacgacccc ttcgatggca agacgctggt catgcacgtg cgcgagtgcg gccccggcga
    13141 ggtcaaggtg ccgttccacg tcggcgacga tcacagccgc acctgggtcc tgacacgcac
    13201 tgacaccggc ctgcgcctca agcacgatca ccgtcacgag gacggtacgg acgacgcggt
    13261 caccatgtac ggcggcgaca cggcggcgcc cggcacggcg agccgccagg aattcccggt
    13321 cgaccagtat tccatcgaca tgttcgtgcg cgagggtctg aacgcctcac tgcagaacac
    13381 ctgggcgatg gagatcgagc cgggcaaacg cttcctctac gagctggcgc gcccgagcgg
    13441 ccggctgttc caggtggagt tcgacctgac ggcgcccgtg gcgacgccgc cgacgccgtg
    13501 gggtcatccg gagatcgaat gacggcgtgc gcctgtcgca agccacggca ccgtcccgag
    13561 ggcattcgca tgaaaaaaat cgccacgaga ttttgccgcg tactgtcggt ggccctgctg
    13621 gcggtgggac tggcggcagt cgccaacgcc ggagactggc gcttcgaggg tgtggagcgc
    13681 gtggtcgcca tcggcgacgt gcacggcgcg cgggacgaac tggtggcgct tctcggcgac
    13741 gtcggcctgg tcgatgcaga gctgcgctgg agcggcggcc gtacccacct ggtgtcggtc
    13801 ggcgacctgc tcgatcgcgg tgattacggg cgccaggtga tggaccttct gatgcggctg
    13861 cagcaggagg cggcggcggc cggcggcgcg gtgcacgtcc tgttgggcaa tcacgaggtg
    13921 atgaacctga cgggcgacct gcgctatgtc agcgagggcg actacgccca gttcggcacg
    13981 gaggcgcgcg ccggcctgcc ggcgggattc ttcgagcgcc gcgcggcgct ggcgccggag
    14041 ggatactacg ggcagtggtt gctgggcctg ccggtggcgc tggtggtcaa cgacacgctg
    14101 ttcgtgcacg gcggcgtctc ccagatgctc gagggaatgt cgctcgagca gatcaaccgc
    14161 gtggcgctgc gcgacgtgcg gcgggtggcg gagggttggc acgcgctcct cggcgccggc
    14221 gtgctgggcg acacggacgg cttcagcaca cttcggtcaa gggctgcagc gctggcggat
    14281 gacggcaggg atgccgcgct gcaggcgctc ggacgagaga tgaacgaggc cctccagggg
    14341 ctgccgttca acccggacgg cgtgctgtgg taccgcggga actcgctctg ccatgcctat
    14401 gccgagggcg agacgctgcg caaggtgctg gctgcggtcg gcgcgcgcca ggtggtggtc
    14461 ggtcacacgg tcaccggccc gcgcaagatc accagccggg tcgacgggct ggtctatcgc
    14521 atcgacactg gcatgaacca cgctgcctac ggcgggacgc cgggcgcgct gatcatcgag
    14581 ggcggcaagc cgctggcgaa gtatatcggc gcgcctgccg cggcggcggc ggtggagacc
    14641 acgcgcgact gggcccgccc ctacggcatg agcgatgcgg aactcgagga tttcctgcgc
    14701 accgcggagg tcacgctggt ggaagatctc gccgagggcg tcacgcggcc gcagcgtctc
    14761 accctcgagc gcgacgggcg aaccatgcgg gcggtgttca agacggtaga caccaatccc
    14821 ggcctggagc ggcagcgccg ctggtcgcgc gagcacgatc tcgccgaccg ccacatctac
    14881 gacgtcgtcg cctaccggct cgaccggatt ctcggggtgg acatggtgcc ggtagccgtc
    14941 gagcgcgaag tgaacggccg gtccggcacg gcgcaatact gggtcccgaa cagcatcaac
    15001 gagaaccagc gccgtgcgca ggagattccc cgcagcagcg actgcagctt cgccccccag
    15061 ttcaacatca tgaacgcgtt cgacctgctg atcttcaata ccgacaggaa cctgggcaac
    15121 atcctctacg acgaggacca gcacatctgg ctgatcgatc acacgcgggc cttcggcacg
    15181 cgacgcggtg agccggcgat gctgcgcaat gcccaggtcg tgattacgcc ggacctggcc
    15241 gcgatgctgg agcaggtcac ggaggagaac ctggagccgc tgaagcccta cctgcacgca
    15301 cgccagatca gcgcgctggt gacccgggcc cagtggttgc gggggcgtca atgaaacgcg
    15361 ggatcgggtc cgacccgtag ctgacgccga aacccgaatc cacgctcagc ccctcgaagt
    15421 cggtggccga cagcgtcacc tccagcgtgc cgctgcgccc ggccggcggt cggatggtga
    15481 agctctggtt gtgggcatcg aagcggatcc agccgggcag cggaccacca tccgcggcgc
    15541 gtgcctggta gcgtagtgcg tcgcccggat cagggtcgat gaaggcccgg tcgggcacgc
    15601 tgaacatcag gacctggccg gccggcacat agacgtccgg cagccgccgg ctcacgactg
    15661 gcggcaggtt ctcgaggttg tggcggacct ggctgcggtg gtacttgccg atggcgttgc
    15721 ccagcgcgaa cagcgcgagt gcgagcgcca ggttgatcgc caccatccag ccgacctcga
    15781 ggccgagaaa ctgcgccgtg agcatgacca gcccggcgct cagcaggtcc ccgaagcgtg
    15841 ccatgaaggt gtcgatggtg gtcttgccga cgtacttctc ctcgcgggtc accggcaggt
    15901 acagcgtgtg gttggtggtg ttgcccatcg acatgttggt ggcgttctcg atgatcatca
    15961 tcacccggac caccgcgaac accggcacga acaggatcag cccgtaattg aggaccatca
    16021 ccgcgggcag caccagtatc gcgccccgca cgccgaacca gcgaaagatc cgtgcgacga
    16081 agaacagctg gatgacgatc tgcagggcgg tgaaccacgt ccagtagttg ccccagaacc
    16141 gggcgatgac gtccccggtg ctgagcacgc cgttgccggc cgccacgacg tcccgtgcgt
    16201 ggccggaaag gaacgtcacg aggacgtacc caccgttggt gttgaccagg ttgagcagca
    16261 tcacgaacag cgcgatccgg gcaaggtagc cgctgcgcgc gacgacccga aagccgccga
    16321 gcacttggcc gatgagattc ccctccggcg ccgcagaagg gccggccggc acgctgcggg
    16381 agccctccgg cacgatgcgt tccgcatggc ggctgaggag tagcacggcc gcgagcagcg
    16441 ccgccgacat caacatcagg ttggtgacgc cgaccatgga gaacagccgt ccgccggcct
    16501 ggctgccggc catcgccccg agcgcggcgc ccatcatgat caccgcgaac aggcgctgtc
    16561 ccgtcttgac gttgaacagg tcggcggcga aggcccagaa ctgcgcgatg accatcacgt
    16621 tgaacacgcc gacccagatg tagaacggca cggcgatcgg aatgccgagc cagaccagca
    16681 gcgcgaagat cagcaggttg gagatgaaga agccgccgac ccacagcagt acggcggact
    16741 tgttgccacg attcttcagg taatcgaaca gcagcttgta gagcgggatg atgaaaatca
    16801 gcgtcaccgc gatggcgccg gtcgagtagg cccgaatctc cggcgatccc tcgacgagaa
    16861 tcagcgtctc acgcacgagg cgaatcacgt agtagccgaa cagcagcagg aagatctgca
    16921 gcagcatgac gaaaatgctg cgaccttcgc ccgggcgcac ggccgtgaac agcgtaagca
    16981 gccgctcgaa aatcgtcagg cgggaaatca ctggcgccga cgaattcatg cttccgtgcc
    17041 tctcagtgct ggcagctcgg gcaatagtag gtcgatctct ggccctgcac cacggcacgg
    17101 atgatgccgc cgcagcgcag gcacggctcg ccggcccggt cgtatgctgc gagctccggg
    17161 cggaagtagc cgcgccgccc ctccccccag gcgaaatccc gcagcgtcgt gcccccctgg
    17221 cggatggccg cctccagcac gtcgcggacg gcctcgacca gcagttcgag ccgcgccgga
    17281 ctgatcctgc cggcggcgcg cgccgggtgg atgccggcgc gaaacagtgc ctcgctggcg
    17341 tagatgttgc ccacgccgac gacgatgcga gcgttcatga tgtgctgctt gatcgccacc
    17401 ttgcggccgc ggcaggcggc ggccaggtaa tccggcgtga atgcggcgcc gagcggctcg
    17461 ggcccgagct cgcgcagcaa cgggtggcgg agcggatcgc cggtcaccca gtcggccgag
    17521 ccgaagcgcc gcgggtcgtg aaagcgcagc acccgcccgg tctcgagcac gaggtcgaga
    17581 tggtcgtgag gctccggcgg ccggtaggtc tgcagcaccc gcaggctgcc gctcatgccg
    17641 aggtgcagca tgagggtgcg gtctccctcg gcgcggaaca gcaggtactt ggcgcggcgc
    17701 tcggcggcca gcaccctgcg ccccggcaac tccgctacca gctgccgcgg caccggccag
    17761 cgcagcatgg gctggcgcaa ctcgacggcc gcgacccgcg cgccctcgat ccagggtgcg
    17821 acgccgcgtc gggtggtctc gacttcgggc agttcaggca ttcttcgtcg cctcgtggca
    17881 ggggcgttgc atgcggtgtg gctggcgggc cactatagtc gcacgattga ttctcgatac
    17941 ggtggacgcc cagtgacgga attccttctc gagcaccatc gcggcctgct tgggctatcg
    18001 gcctggggct acgtgctcgc ctccctgctc atggtgcagg tgaccgtgtt cgcggtgacc
    18061 ctgtatctcc atcgcgacgc cgcgcaccgc gccgtggacc tgcatccggc gattcgtcat
    18121 ttctgccgct tctggctgtg gttgaccacc ggcatcgcga cgcgcgaatg ggtggccatc
    18181 catcgcaagc accacgcacg ctgcgagacg cccgacgatc cgcacagccc gcagatcgag
    18241 gggctgagga aggtgctgct ggagggcgcg gagctgtatc gtgccgaggc ccgcaacgag
    18301 gagacgctgc gcaagtacgg ccggggcacg cctgacgact ggctcgagcg caaggtgtac
    18361 ggcgcggccc gcagctacct cggcatcgtg ctcatggtgg ccttgttcct gctgctgttc
    18421 ggcgtgccgg ggattatcat ggtcgcggtg cagctggcct cgcagccgct gctggcggcc
    18481 gggatcgtca acggcgtggg ccactacgcc ggctaccgga agttcgagtg cgccgacgct
    18541 tcgcgcaacg tcacgccatg gggcgtgctg ctcggcggcg aggagctgca caacaatcac
    18601 cacgccttcc cgtcgtcggc acgcttttcg atccggccgt gggagctgga catcggctgg
    18661 gtcgtgttgc gcggcctggc ggcgatcggc ctggcggaca tccggcgcac ggcgccggtg
    18721 ccggttcggc agccggcacg ccagcagctc gacctcgaga cggtgcgcgc catcatcgtc
    18781 ggccgcatgc acgtgatgcg cgactacacg cgcctggtca cgctgccggt gctgcgcgcc
    18841 gagttcgccg cgctgcgggc ccggataccg gaacgtgccg gtcggctccg gcggctgctg
    18901 gttcgcgaga ccgcattgct ggaccaggcc gcgcgccggc gcctgcacga ggtgctcgag
    18961 agcaatcatg cgctggccac ggtgcatgag ttccgcgagc ggctgaagga aatctgggcc
    19021 ggctcggcga ccagcaacga ggggctcatc gcccagatcc gggactggtg cgcggaggcg
    19081 gaggcgagcg gcgtgcgcgc cctgcaggac tttgccgccg ggctcagggg ttactcgctg
    19141 cgcaccgcct gaggcgggcc gcgcggcggc cggggcgacg ccacgaaaaa gcccgccgaa
    19201 cggcgggctt tttcttacgt gcggctgcaa tccacgcggg aagttacttg atcttggctt
    19261 ccttgtagat cacgtgcttg cgcgccttgg ggtcgaactt gctgacctcc atcttctccg
    19321 gatgcagccg cttgttcttg gtggtggtgt agaaatggcc ggttccggcc gacgacacca
    19381 gcttgatctt gtcacgcatg attccgctcc tcagaccttt tcgccgcgcg cgcgcagctc
    19441 ggacagcacg gtgtcgatgc ccttgcgatc gatgatccgc agcccctgct gggagacccg
    19501 cagccgcacg aagcgctgct cgctctccac ccagatgcgg tgggtgtgca ggttgggcag
    19561 gaaacggcgg cgcgtcctgt tgttcgcgtg ggaaacgttg ttcccggtgg tgggtccctt
    19621 gccggtgacc tggcagattc gcgacatgac tgtaattcct cggaaaaacc ggtcgcccgg
    19681 ccattggaga gtcgggcatt atgccgccgc cgcgagcacg gcgcaagcca ggaaattggg
    19741 gtcagagacg catttcctcg gaaatgcgtc tctgacccca atttcctaca acaggccgcg
    19801 ctcggcgaag gacacgcagg cgccgtcacc gaccaccagg tggtcgagca cgcggatgtc
    19861 aaccagcgcc agcgcgctct tcaggcggcg cgtgatgacc tcgtcggcct ggctgggttc
    19921 ggccacgccg gaggggtgat tgtgggccag gatcagcgcg gcggcgttgc gcgccagggc
    19981 gcgcttgacg acctcccgcg ggtggacgct ggcgccgtcg atggtgccgc gaaacagctc
    20041 ctcgaaatgg atcacccggt tgcggttgtc caggtgcagg cagcaaaaga cctcgtgcgg
    20101 caggtcccgc aggcgcgccc gcaggaagcg ccgcaccgcg tccggcgagc cgagcgccgg
    20161 cccgcggcgc accgtctcct cgaggtggcg gcgcgccagc tccaggcccg cctggagctg
    20221 cacgtacttg accggcccca gcccgggccg ggcgacgaag ctggccaggt ccgccgtcag
    20281 cagcccgcgc aatccgccga agccgagcag cagttcgcgt gccaggtcga ccgcgctgcg
    20341 gcccggcgtg ccggtgcgca ggaagatagc cagcagctcg gcgtccgaca gtcggtcggg
    20401 cccgccggcc agcagctttt cgcgcggccg ctcggccatc ggccagtctc tgatggggag
    20461 gtggggcatg gtgctgggcc agtgtcgggg tgccaatccg tgggccactc aggttaagct
    20521 gccggacgca gtcaaggcat ggagccttta tgcccactct tgcgggaaaa caggtgcttc
    20581 tcggcgtcac cggcgggatc gccgcctaca agtcgcccga cctggtccgc cgcttgcgcg
    20641 agcagggggc tgaggtgcgc gtggtgctga cgccggccgc ggagcgtttc gtcacgccgc
    20701 tgaccttcca ggccgtgtcc ggccacccgg tgcgcggcgg cctctgggac gagtcggccg
    20761 aggcggcaat gagccacatc gagctggccc gctgggcgga cctggtgctg gtggcgccgg
    20821 ccaccgccga tttcctcggg cggctcgccg ccgggctggc cgacgacctg ctggcgacca
    20881 tctgtctcgc caccgaggcg ccgatcgtgg tggcgcccgc catgaaccga ctcatgtggg
    20941 ccaacccggc gacccaggac aatgtccgcc ggctggccgc ccgcggcgtg acggtgctcg
    21001 gtccgggcag cggcaaccag gcctgcggcg aaaccggcga ggggcgcatg ctcgagccgg
    21061 cggagatcgc agccgggctg ccggtggcgg ccggccgccg cgtcggcgtg ctggcgggga
    21121 ggcgcgtgct ggtcacggcc gggccgaccc gcgaggcgct ggatccggtc cggtacctga
    21181 ccaaccgcag ttccggacga atgggctatg cagtggctgc ggccgcggcg cgcgccggcg
    21241 ccgaagtcgt gctggtaagc gggccggtgg acctggcgcc gcccgccggc gtgcgcgtgg
    21301 tgccggtgga caccgccgct gcaatgcgcg ctgcagtgca ggcccagctg cccggcaccg
    21361 cggtgttcgt cgccgcggcg gcggtcgcgg actaccggcc ggcggcgccg gccccgcaga
    21421 agatcaagaa gggcgccgcc gagatgacgc tggcgctgct gcgcaatccc gacatcctcg
    21481 ccgaggtcgc ccaggcctca ccgcggccgt tcgtagtcgg cttcgctgcg gaaacggaaa
    21541 acgtcgaagg caatgcacgg ggcaagctgg agggcaagtc gctggacatg atcgccgcca
    21601 accgggtggg gtcggattgc ggcttcgatc gcgaggacaa cgccctgctc gtgctgtggc
    21661 cgggcggtgg ccgggaagac ctcggcagcg gcagcaaggc cgaactcgcc gaccggctcg
    21721 tccgactgat cgcggagagg atggatgcag gtcatcaagc tcaaggttct tgattcgcgc
    21781 ctcggcgcgg aatttccgtt gccggaatac gccacgccgg gttccgcggg cctcgacctg
    21841 cgcgcctgcc tcgatgcgcc gctggtgctg cagccgggtc agaccgagct gatccccacc
    21901 gggctggcga ttcacatcga cgacccgggc ctggcggcgg tgctgctgcc gcgctccggc
    21961 ctgggccaca agcacggcat cgtgctcggc aatctggtcg gactcatcga ttccgactac
    22021 cagggtcagg tattcgtgtc ggcctggaat cgcggcgctg cggagttcac cgtgcagccc
    22081 ggcgaacgga tcgcccagat ggtggtggtg ccggtggtgc gcgcccgttt cgagatcgtg
    22141 gcggattttt ccgccagcga gcgcggcgcc ggcggcttcg gccactccgg ccgccactga
    22201 aggctgttgt ccggacctag cgttccggcg gcgtggcttc ggcgcgcgcc gcgctctctt
    22261 cgctctggtc ttccgccgaa ccttcggcgc cgcggagctc ctcgaagcgc cggatgtctc
    22321 cctggaattg atcccgaatg cgctcgatct cggcgttgcg gcgggagatc tcgtagacgt
    22381 actcgcggat ccgctgctcg acggtggcga gctcgttgat cgccgccgga ttgtccgcca
    22441 gcgcctcccg tcgggcgcgg aggctctgca ggcgatccag cgcgacttcg ttctggtcgt
    22501 cgaggtagcc gatgcgccgg tcgcgcaccg actcgatctc ggccacggag ccgtaggtcg
    22561 ccagcagcgc ccggtcgcgg ttgtcccgcg cgggcgcgtc cggctcgggc ttttcgccgt
    22621 ggaccgtgac gcgcgtgccc cgtccgtcca gcacctcgtg gcgctccttg gagtattcgg
    22681 gggggatggc gtcgccgtaa tggaccacgc cgtgctcgtc gacccagcga tagactttgc
    22741 cggcggcggc cgccggaacg gccgtcgcgg cgcaggccgt cgccaggatc gccgccagcc
    22801 agaagtctct cgtcttcatg cgcatgacct ccgcgtcacc cgacgcgtca ccggtacatc
    22861 ataacgccat gcccgctgcc gtagtgtgcg ggctatcacg cgacgccgta gcgccggcgg
    22921 tactcctgca gcgcggcaag ccgctcgggg tggtcgccgc cgccgtgctc caggtgggcc
    22981 agcaggtccg ccaggctgat gatgctggtg acgggcagcc ccaggtcctg ttcgacctcc
    23041 tgcacggcgg aacgttcgcc gcggcccctc tcctgccggt cgagcgcgag cagcacgccg
    23101 cagggctcgg cccctgccgc gcgaatgatg ccaatggatt cccggatcgc cgtaccggcc
    23161 gtgatgacgt cgtcgacgat cagcacgcgt ccagccagcg gcgcgccgac caggttgccg
    23221 ccctcgccat ggtccttggc ctccttgcga ttgaacaccc agggcaggtc gcggccgtgc
    23281 cgcccggcga gcgccattgc agtcgccgtg gccagcggga tgcccttgta ggccggtccg
    23341 aacagcacgt cgaactgcag ccccgcagcc acgatggcat cggcgtagca ggcgccgagc
    23401 tcagccagcg cggcccccgt attgaacagg ccggcattga agaaataggg gctctcgcgc
    23461 ccggatttca acgtgaagct gccgaacttg agtgcggccc gggcgagggc cagctcgatg
    23521 aaggcgtgct tgtaactgtg catcgacgcg tcctgccgcg aggaatttgc gccgcgcgcg
    23581 gggcgtcgcg gcggaccgac ggtatgatac acgccggacc cagcaccccg gccgacatgc
    23641 gcatcatcag cctcaacgcc aacggcatcc gctccgccgc ccgcaagggt ttcttcgagt
    23701 ggctggcggg acaggacgcc gacgtggtgt gcctgcagga gacgcgcgcg cacgagcacc
    23761 agatcagcga cgacgcgttc cgcccggccg gctatcactg tcattacctg gcggccgagc
    23821 gcaagggcta cagcggggtg gcgctgtact cgaaacgcgc gccggagcgc gtcgtgcgcg
    23881 gcttcggttc ggccgagttc gatccggagg gtcgctatat cgaggcggtc ttccccggcc
    23941 tgtcggtcgc ctcgctgtac atgccctcgg gctcctccag cgaggagcgc caggcggcca
    24001 agtaccgctt cctcgacgag ttcgaggatt tcctgcgccg tgtgcgccgc cggccgcgcg
    24061 agtacgtcat ctgcggcgac tggaacatcg cgcacaagga aatcgacatc aagaactggc
    24121 gcggcaacct gaagaactcg ggcttccttc cggaggagcg cgcctggatg gaccagctgt
    24181 tcggccgctg gggcttcgtg gacgccttcc gggcggtcga tcctcgcccc gagcgctaca
    24241 cctggtggtc gaaccgcggc cgtgcgcgcg agaaaaacgt cggctggcgc attgattacc
    24301 aggtggtcac tccgggcctc gcggaccgca tccgcggcgc gaacatctac accgggcacc
    24361 gcttgtccga tcacgcgccg ttgatcctcg actacgattt cgacctgccg acgtgatcgc
    24421 caccctcagg cgcctgctcg agccttaccg tgaccggcgc atggcgaccc tgctcgcgct
    24481 cggcttctcg agcggcctgc cggcgccgct ggtgttcacc aacctgtcca tctggctgcg
    24541 cgacgacgga gtttcgcgca ccgacatcgg cctgttcgcg ctggccgcga cgccgtacgc
    24601 catcaatttc ctgtgggcgc cgctggtgga tcgcatgcgc ctgccgttct tcagcgcgag
    24661 gttcggccgt cgtcgcggct gggcgctgtt tacccagctg tcgctggtgg cagcgatcgt
    24721 ggtgctgtcg cgcgcccagc ccgcggcgga cctgttcgcg gtcgccgccg ccgtgctgtt
    24781 cgtgaccttc gtgtccgcga cccaggacgt ggtcatcgac gcctatcgca tcgacgtgct
    24841 gtccccggag cagtacggcg cgggctccgc ggccgccatc tggggctggc acctgggcgg
    24901 cacgctgatc ggcggcgccg gtggcctgta cctcgcgcag gtgttcggct ggaacacggc
    24961 ctatctgttc cttgcagcgg gcgtcggcgt cggcatgctc gccatcctgc tcagccccga
    25021 gccgccgttc cgaccgccgg ccgacacgct cgagcacgag gcgcgagtgg cggagctgct
    25081 gcgccgcgtg tcgtggctgc gcgggcgcct cgcgggcgcg gccgcgtggc tgtacggcgc
    25141 ggtggtggcg ccgttcgtgg acttcatgaa gcgcgagggc tggctgctgg tgctggcgtt
    25201 catcatcgtg ttcaagttcg gtgacgcgct gctcggacgc atgtccggcg tgttctaccg
    25261 tgagctcggc ttcagcctgg tggagatcgc ggaggtgatg aaggtttacg gcttcatcgc
    25321 caacggcatc ggcgtgctgg cgggcggcgt gctggtggcg cgcatcggcc tgttccgggc
    25381 gttgctgatc gccggcctgc tggcggccgc gacgaatctt agctactcgt ggctggcaat
    25441 gtccggcaac gatcccgtgg tgttccgcat cgcggtcatc accgacaact tcacgggcgg
    25501 actggcgacg gtggccttcg tcgcctacct gtccagcctg tgcaacgtcg cttacacggc
    25561 cacccagttc gcgttgctgg cctcggcggg caacctcggc cgcatctggc tgtccgcctc
    25621 gagcggctgg atggtggatg cgctggacgg cgactgggcg gtgttcttcg ccatgacggc
    25681 cgggatcgcg ttgctgggac tgccgctctt gctggtgctg atgcggcgct acccggaaac
    25741 gcaggcgcgc gcgcccgagc ccgagcgaga ctgaccgacc gcgacaccgg cagtcgcgcc
    25801 gggctgccgg gtctgccggc aggcccctgt tgacgcaaac cggtttgtat tgcaaactag
    25861 tttgcgttga cctggaggtg cctgatggcc atgaacctcg atgacctgga ccagctggcc
    25921 gcccgcgcgg cgcgctatgc gcgttactcc cgcagtgccg gcggactcag ctcggtgatc
    25981 ggcggcctgc tgctgctggc cagcttcgcc gccaacgcct tcgccgagct cgccttctgg
    26041 caacgcatcg cgctggcgct gacgccggcg ctctggctgc tggccaagga gctgctgcgc
    26101 gccaggtatt accagcgcga cggccgggcc ctgcaggcgc ccacggccag ggagcggcgc
    26161 aatcatcgcc tgatcgtggc ctatctcgcc gtgatttcgg tgatcgtgct gggggtgttc
    26221 gtccatgcgg cactggaggc cggccgactg cccggtccgg gcatgcttgg ctacgcggtg
    26281 atcgttgccg tgctgcccgt ggtggcctgg cgctggttct ggtccgtcag cgacttcctg
    26341 gtcggcgtgc tgctgatgtg ccagtcggcc gtggtgatcg gtggcggtca ctacccggcg
    26401 atctggctgc tctatgtcgg cgcctgcgcc gcgatcgcgg tcctgtacgg tctgcgggag
    26461 caccgtgaat acctcgccct gcgggccgaa ttcacggcgc ggacaccgct gcaatgaagg
    26521 acagcacgcg cagcgcgatc gacggcgtat tgaacgtcga ccggctggtc cacgagccgg
    26581 cccggctggc catcctcacg gtgctgagcc aggcggagtt cgccgagttc ggcttcctgg
    26641 agatggcctg ccggctgagc aagggcaatc tctccagtca cctcagcaag ctcgaggcgg
    26701 gcggcctggt gaccatcgag aagagattcg aaggcaagcg cccgcagacg cgcgcggcca
    26761 tcaccgaaag cggccgagcg gcgctggcga actaccgcga gcacctgaag gcgctcatca
    26821 gcgaggtgcc cacgggcgcc gaaggagacc agcaatgaaa cgactcacgg gccgcctggc
    26881 cattacattc ctgtggtgca tgtcgctgca cgccaacacg gcggcagccg cggacgaggt
    26941 cgcggcgcga gagaccgcgc tggcggacgg acgcgcgctg acggcgcagt ttctcgcggg
    27001 ggaggccgat gccctctggc cgcggttcgg cgcgcagttg cggggcgtga tcggcagccc
    27061 cgcggggctg cggggcttca gcacgcagat ccgcgagcaa ctcggcgcag aggacgaggt
    27121 gctgcgcgag acggtgcagc gccagggcga gttcaccgtc taccagcgca tcggtcgctg
    27181 gtccctggcc ccgatgccga tcgccgtcac ctgggcgttc gggccggacg ggcgagtcga
    27241 gggcttccag gtggcgccgg cgcccgccga agcgcccgcg gccgatcccg cccgggcgcc
    27301 ggttgagtcc gggcgcctgc cggacgacgc gacggttgcg gcgcaactgc aggctttcgt
    27361 cgaacaggca ggtgcggcgc ccggcgtggt cgtcggcctg cacgacgcgc agggcacacg
    27421 gttccaggcc tggggagacg ccggcgacgg cgcgccgccc gatgcggaca cggtgttcga
    27481 ggcgggttcc atcaccaagg gactcaccgg actgttgctg gcgcagatgg tcgccgccgg
    27541 ggaggtcgaa ctggagcagc cgatcggcga cttgctgccg gacggcctcg agctggcgcc
    27601 cgggctggcg gccatcaccc tgggggagct ggccacccat cgctccgggc tgcctcgcct
    27661 ggcgagcggc ccggagatgc aggcgcgcat gaccagcgat gatccctatg cgggtagcac
    27721 cactgtcgag atcttcgccg acctcgctcg ggtgatgccg gaaaccgtgg ccgccggccg
    27781 cggccgcttc gcctactcga acctcggcac ggcggtgctc ggccagctgc tggcacgctc
    27841 ggtcggccag ccgtacgagg acctgctggc cgcaagggtg ttccaagccc tcgacctgcc
    27901 cggcccggtg ctcgacccgg acgcggtcac cgggcggcgg gcggtcggca cgcaggccgg
    27961 tgcgccggtg aaggcctggc gcttcgatgc ctatgcgccg atgggcgcct ggcaggccag
    28021 cgcgcatgag ctggtcgcgc tcggccgacg cctcctgcag acggagcccg actgggtcgg
    28081 cgcggcgctg gcgccgcagg ccgtgccggg ccacccgggc accggcatgg gactgggctg
    28141 gcatcacgcc gaactcggcg cgcgccgctt cgtgtggcac aacggcggca cggccgggtg
    28201 cagcagcttc ctggccgtcg tgccggccga aggcctggtg gtggcggtgc tggccaacgg
    28261 cggcggcggt atcgccgacg gcctggcgcg cgaattgctg gtctcaggtc gttgatcgtt
    28321 cgggcaccgc gctggtgcgt gagccggttc gcgccggccg ccgctagtgc acgggtccgc
    28381 gggcgctggc gccgcgatgg atcggcccgc cgtggtgtgt gcgcagtttc cagccctgct
    28441 cggtcagcgt atagacgttg gtcacggtca ccagcgcagc atcactgtcc ggttggccga
    28501 tgacttcgat gcccacgtgc accgcgaggt cgtcgccgcg cacgcggctc tggcagtcaa
    28561 agcgcacctg cacgccgccg gtcgcgccga gaatctcctc ccagctctgc agtaccgcgc
    28621 gcctgccggc cagcggcggg cggcccgggt ggatgcaggt gacttcgccg tccatgcccc
    28681 acacatgctg catcgaggca aggtcgccgt cgcgaaaggc gtcgtagaac gcggcctcgc
    28741 aggcctcggg gctcgcgtag ctgccggtcg gcaagcctta ttccgcctgg tccaggaaat
    28801 ccagcgggtc ggaccagtcc cggacggcgg cgcggtcgag atccatcccg tagtcgtccc
    28861 gctcgatatc cagtgagccc gagtagtcct ccggcggatc ggccggcagg aattcgcttt
    28921 cttcccaggc gtcgaattcg atctcctcga cggtgccgtc gaagtgctgg atgtcgatgg
    28981 agccgtcctc gtcgtcgacg gcgatcactt cgaaccggac ccccgtggga gcctggtacc
    29041 agtcaccgat cgccggaatg ggcagtggca taatgatttc cccgttagtc cgggcctgtt
    29101 atccttgccc gccttcagca ggcccgccct gccaaactca gcttccttgc gacaacttat
    29161 agctcagccc gaccgggctg caagtctcgt acctcgcgta ttggccccga aatgaccgag
    29221 cagctgatca agacgttcat catttttttc gtggtcatcg agcccgtctc gctggtcccg
    29281 atgtttggtg ccctgacgcg cggtggcgag ccgggttacc ggacgcggat ggcctggaag
    29341 tcggtggcga tctcggccgg aattttcatc gtgttcgcgc tggccggcga ctggctgctg
    29401 cgccttctcg gcatcagcgt gtcggcgttc aagatcgccg gcggcgtact gctgttcctg
    29461 ctggccgtgg atatggtgct ggcgcgtgaa tctggtctgc gctcgaccac cgtgcgcgag
    29521 caggaggagg cgcgctaccg cgaggacatt tccgtgttcc cgctggcgtt cccgctgatc
    29581 accggcccgg gcgcgctcgc cacgctgctg ctgttgaccg ccgatacggc caactacatc
    29641 gagttctcgc tggtgctcgg catgggcctg ctcgcgctgg cggtgacgct ggccttcctg
    29701 ctgctcacgc agccgctgat gcgcgtcatg ggcgtcaccg gtggcacggt cgtcagccgc
    29761 ctgctcggcg tggtgctcgc cgcgctggcc gtgcagtaca tggtggacgg cgtcaccgaa
    29821 gtgctggcgt aagaaagccg ggaaaactgg ggtcagaccc ccttttcggc cccgaaaagg
    29881 gggtctgacc ccagttttcc cctgacccca attatgccta ttgccttcgg tacaacaggt
    29941 cgaagacctg gtggccgagt ttcaggccgc gacgctcgaa gcgggtcacc ggccgggttt
    30001 cggaacggtc ggcgtagcca cggccaggcg ccgcgttctc gaagtgcggc gaggcctcgc
    30061 acacttccag catct
//
LOCUS       DAOMPG010000004        27465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_755_length_27465_cov_4.761328, whole genome shotgun sequence.
ACCESSION   DAOMPG010000004 DAOMPG010000000
VERSION     DAOMPG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 27465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27465
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_27465_cov_4.761328"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..442
                     /locus_tag="PLS34_00470"
     CDS             179..442
                     /locus_tag="PLS34_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRP85976.1"
                     /translation="MFKALADPTRREVLKRLQRGPATAGELAEEFPQAKATLSHHFNV
                     LKAAGLVRAERMGQHVVYSLNTSVFEELARMMAELFKPERKAK"
     gene            439..1101
                     /locus_tag="PLS34_00475"
     CDS             439..1101
                     /locus_tag="PLS34_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRP85977.1"
                     /translation="MSARLTQWISLGLVLAALAVSAALYGDLPSQVATHFNAAGEPDG
                     WSDRPTAALMMPAIMAGLWLMLWVLPKISPQGWRVEPFADVWSRCQLAIIAFMLLVHV
                     GVLGHAVGWWGADLGGMVMIGVGLLFATLGNYLGKTTRNFFLGIRTPWTLASDEVWRR
                     THRLGGWLMVGAGVALMVMGFTGVHELAIAVVIGVAVLVPAIYSFFAYRSIEGFKPPP
                     DH"
     gene            complement(1098..3116)
                     /locus_tag="PLS34_00480"
     CDS             complement(1098..3116)
                     /locus_tag="PLS34_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HRP85978.1"
                     /translation="MKIPLMGGLLLAALHTTTWAATEAADPALAATESQPPEATAAAD
                     YAQLEPVVVTAVPMRDPYTIVTDPRQPRLPLPAHDGGAYLKSIPGFTVSRKGGTSGDP
                     ELRGLGGSRLNILLDDTHVLGGCGGRMDPPTAYVFPESYDRIEVLKGPQSVRYGATIA
                     GIVRFDREPTRFTERTVTGFGSLIAGSHDRRDFVGDVTAGDRLGYVRMIGTLSTQDDY
                     EDGAGRTVHSQYHRWSTTGMLGWTPDELTRIEFAAERSDGEAAYDDRGMDGVVFDRTG
                     YTLSFDRRDLAPWFTRLEALLYYNYVDHVMDNYTLRVPPMQPMVSYPDRRTVGGRLPG
                     EFQVSPALEIVAGVDWAENEHGSNQLMGPAAFNYRNVPREDNAEFTDSGLFVELERAL
                     GEHSRFNAGVRADRRKSTALDGINFGGAAPNTSRTSDQQSAFMRLSRDLSGRPVTLYA
                     GLGRAERAPDFWEMRRVFGLDNETLTQLDLGVSLRSDKVTANLAVFAGRIDDYILIVA
                     PGLEAVEARNVDAKTSGLEADLNYRLRPGFSTTVTAAWVRSENDTDGVPLAQTPPLQG
                     TLSLDYEKERYFGGLLFRAVARQDRIHAGYGTIYSLDSGESPGFAVLSGYGGYRFGQG
                     LTATAGVDNILDRAYAEHIQRGSADLGASSRRIFEPGRTLWLRLAAEF"
     gene            complement(3222..3599)
                     /locus_tag="PLS34_00485"
     CDS             complement(3222..3599)
                     /locus_tag="PLS34_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP85979.1"
                     /translation="MKPWLRQPLLNSILLLALFLRALVPDGFMPAKGELVELCTLHGP
                     RTVLADPATGELLQDEDQDDGATATCPWSLLLSPLAASSFTSPTGALDKAPAAVPEIA
                     LPPISRPSLIVPPARAPPIPVLS"
     gene            complement(3871..5493)
                     /locus_tag="PLS34_00490"
     CDS             complement(3871..5493)
                     /locus_tag="PLS34_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP85980.1"
                     /translation="MAKPQDLDALVIGGGHNGLVCAAYLAGAGLKVRVLERRPTVGGA
                     AVTEEFHPGFRNSSCSYTVSLLNPRIIAELRLAEHGLRIVARPLANFLPLRDGSAFRF
                     GGASTQAEVGRFSRRDAERLPEWFAMLERVVVVLRALAEQTPPNVTDRFVLADWLASW
                     AVARRLKGLDMSGRRDLLELFTRSAGELLDDWFECDPLKAVLGWDSIVGNFASPYAPG
                     SAYVLLHHVFGEVNGKPGAWGHAVGGMGAISEAIATEARARGVEISTDAAVERVLVRG
                     GRAVGVALADGREIRARRVIAGVNPKLLYTRLFDPAELDPELLARFGRYRCASGTFRM
                     NVALAELPDFRCAPGTQLQPHHASGILVGDSLGYFERAWFDARSREHNPGWAREPVVE
                     LVISSALDESLAPPGMHVASLFCQHVNPEPDGGWDAHRETFADLAIETVNRHAPNFSA
                     SVLGRRILSPLDLEREFGLLGGDIFHGALGLDQLFSARPLLGQADYRGALPGLYLCGS
                     GTHPGGGVTGLPGRNAAREILRDTAGRTLKTT"
     gene            5630..6499
                     /locus_tag="PLS34_00495"
     CDS             5630..6499
                     /locus_tag="PLS34_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRP85981.1"
                     /translation="MIARQGSVPGGDRIRRLNSMKKGFVIGLLAVVLVACATSPTGRR
                     QLMLVSPDQAISASRTAYVQQMRAFDEKGLISTDKALVARINQITGRLVYQAIQMYPA
                     TADWEWSMEVIDEPETVNAWAMAGGRMAIYTGLIEKTKATDDEIAQVIGHEIAHALAN
                     HTAERMSVVMATQLGVLAVGVSTRDDRAMAGAAAASLLAINLPNSRTAETEADRIGIE
                     LAARAGYHPDAAASLWKKMAEVGGGGPPQFLSTHPAPGNRQQTLARLAPQMMQYYEAP
                     GERPVHPMRPGRY"
     gene            complement(6561..6806)
                     /locus_tag="PLS34_00500"
     CDS             complement(6561..6806)
                     /locus_tag="PLS34_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRP85982.1"
                     /translation="MNTVDIEQVMRAALGSVAPDLELDQLDPELLIRDQADFDSMDTL
                     NFAIALSRSLGIDIPESDYPRLASLADCHRYLAARLG"
     gene            complement(6803..7921)
                     /locus_tag="PLS34_00505"
     CDS             complement(6803..7921)
                     /locus_tag="PLS34_00505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRP85983.1"
                     /translation="MADFLMPSLGADMDEGTLVEWRVKPGDAVRRGQVVAVVETAKGA
                     IDVEIFEDGEIETLLVEEGAKVPVGQPLARLRGAAPQLRASPAARRRARELGVALEGI
                     DGSGPEGAITLADVEAAGAGRARSAANAPRTSAEPAATASESPASMTPASPADMRAVI
                     AAAMTRSKREIPHYYLGHWIDLAPANKWLDEWNAARPVEERLLLAVLVIRAVALAAKS
                     YPEFNGHFRDGAFVQGDGIHVGSVISLRRGGLVAPALRDADQRSLPELMAAFRALVAR
                     ARAGRLTAAELAGGTITVSSLGEQGVEEVFPVIYAPQVAIVGVGTPVQRPWVADGRVE
                     PRWLLRTTLAADHRTSDGHRGALFLDRISRLMQEPGSL"
     gene            complement(7914..8888)
                     /locus_tag="PLS34_00510"
     CDS             complement(7914..8888)
                     /locus_tag="PLS34_00510"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRP85984.1"
                     /translation="MKTTYREAVRGAIRAAMHADERVFLVGEDVGRYGGSFGVSMGLQ
                     EEFGERRILDAPLSESGFTGAGIGAALGGMRPIVEIMTVNFSLLALDQILNTAATLRH
                     MSGGQFSVPLVIRMATGAGRQLAAQHSHSLECWYAHIPGLRVVAPATLEDARGMLPAA
                     LADPDPVIIFEHIGLYNMEGELEEDAGAVDLERAAVRRVGTDLTLITYGGSLRKSLDA
                     AERLSADGIDTEVIDLRSLRPLDDETLTASIARTRRAVVVDEGWRTMGLSAEIVARLV
                     EHCFYELDAPIARVGTEEVPIPYPAHLEQAALPSVDKIVAAARRVTGG"
     gene            complement(8894..9883)
                     /gene="pdhA"
                     /locus_tag="PLS34_00515"
     CDS             complement(8894..9883)
                     /gene="pdhA"
                     /locus_tag="PLS34_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRP85985.1"
                     /translation="MSDPAPIALCKQHGLDLLRAMERIRRFEDRAAELYTAMKIRGFL
                     HLYNGEEAVAVGVMGGLRPGDAVIATYREHGHALAAGLPMGAVMAELFGKVEGSSGGR
                     GGAMHIFDAEHRFYGGQAIVAGGLPMAVGLALADRMQERARVSACFFGEGAAAEGEFH
                     ESLNLAALWELPVLFICENNLYAMGTALARSQAVTDIHARAASYGVESSVVDGMDVLA
                     VEAAAGAAAAAIRRDQRPRFIECRTYRFRAHSMFDAQLYRSREEVERWKQRCPILALR
                     RRLLADGLASDEEVAVIEREVAGEVEQAVAFAEAGTWEPVETLARHVTAERAP"
     gene            complement(9880..11658)
                     /gene="acsA"
                     /locus_tag="PLS34_00520"
     CDS             complement(9880..11658)
                     /gene="acsA"
                     /locus_tag="PLS34_00520"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRP85986.1"
                     /translation="MRFEIFHRSHVRFGEPPPNMTDYRSACAAFSWAQAERQLEGLPA
                     GGLNIAHEALDRFVSQGLGERLAMRFIARDWGREDLSYADLARLSDRFANVLHELGVA
                     PGDAVFSLAGRTPELYVTMFGALKARAVYCPLFSAFGPEPVRSRLELGSGRVLVTTRS
                     LYQRKVLPIRGALSKLEHVLLIDAAAGELPPGTLSYPELMAAASDDWEIPATDPESIA
                     LLHFTSGTTGKPKGVMHAHRAVVAHHATGRCALDLHAGDVYWCTADPGWVTGISYGAI
                     APLVNGASMIVDREEFDAARWYRILQEEGVNVWYTAPTAIRMLMRQGNELATSYCYSR
                     LRHIASVGEPLNPEAVRWGRDIFGRPIHDTWWQTETGAIMIANFRASRVRPGSMGRPV
                     PGVEAAIVRVSRADDDDPPAIEIITAPGIEGELALRAGWPSMFRGYLNDEARYRRAFA
                     DGWYLTGDMARCDADGWLWFVGRADDVIKSAGHLISPFEVESALMEHPAVAEAGVIGL
                     PDPVAGETVKAFVTLKQGVAGDDHLALALIGFTRKRLGAALAPRQVEFRDELPRTRSG
                     KIMRRLLKARELGLAEGDTSTLESGR"
     gene            complement(12039..13172)
                     /locus_tag="PLS34_00525"
     CDS             complement(12039..13172)
                     /locus_tag="PLS34_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HRP85987.1"
                     /translation="MSTIFRIVILATLLVVLAIGGLRLHFGGGGPFPEMAGQPSVTES
                     GLETVLSHPEPLGNVAVSATGRLFFTVHPEARTDGPRLYEWRGGRAEPFPDEELQAAV
                     LETPLGLAIDRQNRLWVIDPAAHGLGRPRIVAIDLDNDRIVHEHVFPRRVAPRGSFLQ
                     DLQVDRDGRMVYIADASVWRKSPALVVYDTLTRRSRRTLEDHPSLRAQDWIIRTPMRE
                     MRFFGGFVPMKIGLDGIALDPAGQWLYFGAMTHDTLHRIPTGALLDDALDSRALAERI
                     EAVGRKPLSDGLSADLSGNVYITDVEHGAVLRMAPGGELTTIVRSPRVRWADGLSFGP
                     DGWLYLADSALPEVVMKTRGHIREHGPYHVYRFRPGTTGVPGQ"
     gene            complement(13279..13707)
                     /locus_tag="PLS34_00530"
     CDS             complement(13279..13707)
                     /locus_tag="PLS34_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP85988.1"
                     /translation="MPCPVRKNMAPVSGSSAEAQQATICVVDGDARERAALSQLLGNL
                     QHDVRAFESAEALLDALDDMSMRVLVAGLELPGMSGLELLHTLRERGRQVPTILLAAE
                     SDVATAVGAIRAGAVDFIQKPAIDRILLRRVQDALEHSAA"
     gene            complement(13841..14464)
                     /gene="fixJ"
                     /locus_tag="PLS34_00535"
     CDS             complement(13841..14464)
                     /gene="fixJ"
                     /locus_tag="PLS34_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HRP85989.1"
                     /translation="MASSEATVFIVDDDEAVRDSLGLLLRSAGYRARCYAGARDFLKA
                     FDPRDYGCLVLDIRMPGMTGLELQKHLAEIGCNIPIVFITGHGDIPMAVEAVRQGAVD
                     FLQKPFQDQELVDRINDAMKLAAEQREGELERLEILDRIESLTAREKQVMGQVVLGKA
                     NKVIAGDLGVSQRTVEIHRARVMEKMQANSLAHLVRMVMVAENHSGA"
     gene            complement(14464..16791)
                     /locus_tag="PLS34_00540"
     CDS             complement(14464..16791)
                     /locus_tag="PLS34_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP85990.1"
                     /translation="MNSRDTPTASPTRKDIPRAEYRLSALLDATPDAVILINGDGVIE
                     SFNPGAERVFGYSAAEIVGRNVALLMPTPFRAGHDGYIQRYQQTGEARFMLAPREVEA
                     CRKDGTVFPAEVSVGRVGDEGTTSFIGIIRDISARIAAEARLHQVEANLLTAQALAHV
                     GSFEGDWPESRSNFWSDEFFRIVGLDPDTQALGPAEFVRRFAHPQDRDRVEARLAEDA
                     RKTSGGGELEYRIVRPDGTVRHIQTIYQIVTNPMSGERRIQGTILDQTFRRHAEEALR
                     RERDRAQRYLDLVNVMIIAMDRSGRITLVNRKACELFGYPEHELLGKDFFAACQPPGF
                     AGRSRILFEEALAGREESFSLMEGPITTRDGHHRRVFWRNQFLRDDSGRVNGVLSAGE
                     DVTEQRETESQLRQAEEELRLIFRRAPVGMATLAAAHEIDGRYLSVNQALCNMLGYTE
                     GELLSRSVRDVTPPEDIGETQRHFQLLLTGGDTVKYDKRFLHKDGSIVHALVNLSLIS
                     DHDGRPLLIVSQILDRTDVVQAEMEARKQRERLAHVARLGTLGEMAAGIAHELNQPLA
                     AIANYTQACQRLLAAGDMEPDELGQVLGRVTAQARRAGDVIHRLRAFVRRHTPDRRHR
                     DANELIQEILPLVEMDCRAHEVELELAFQVDLPKVQVDGIQLQQVLLNLTRNAVEAMN
                     AVEAETRRLVVRTVALNEDEVELAVEDTGPGVADGLREQLFEPFFTTKPEGMGLGLSL
                     SRSIIEAHGGTLRYDSGTEKGSVFRIRLPTAPEEE"
     gene            16941..17921
                     /locus_tag="PLS34_00545"
     CDS             16941..17921
                     /locus_tag="PLS34_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRP85991.1"
                     /translation="MKKFSRILVGIDPHDTSLHAAALGARLAAGTGAALELFTPVFNS
                     QISLAHFENRQSLQHARDLLVARHRERLEAHLAGLGIDGTVECHAAWDHPIEEALVRR
                     VLERGADLLVVALPVGAGGGRPLSAAQWQLVRHCPSAVLLTRGGAWRDRPTIVAAVDP
                     SGHRGRHDALDIRILQAAGRLADGVSGTVHAFHAWQKSLRALIGEVELPLATGTAETG
                     VEDRHREAVYAVLAAADASPARVAFVAGRPETALPEYCREVEADVVVMGAIARNPFGR
                     ILLGSTAERVLDRLPCDLLVVKPEAFRSPVSRERWPLDEDGMTILGVPGI"
     gene            18001..20124
                     /locus_tag="PLS34_00550"
     CDS             18001..20124
                     /locus_tag="PLS34_00550"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP85992.1"
                     /translation="MAQSLRILLIADDPELIRRIGHGLQRQDSGLIMIEGADTLATAR
                     RRLGAGTYDLALVDLSLGHGDGLHLLSDLQEIAPELPIVALAANGNGPDAAACLALGA
                     QDRLAPEAMDSAGLLDRLHSARARAQAGLRLRRRSHRIAASLGATGDLAWHYTHGEGE
                     VWLAAANPVEWQLPAPECSESLDALRARIHPDDRELVLRQLEEVVLSSQPWQLDARFK
                     VDGGAYRWCILRGRSQLDGRGALQYASGVLSDAQRQQKRLREVEQGRRFLRAVFDSAR
                     VPQAILDSSAVISDCNQAWVALDEPACHAGSAFGPGRRFIDKPEDPAAYGDLDHAELA
                     RGVRQVLGGVADQFVCEYGDSERRWRMTVTPLLNPGIAGAVVRHEEVTETRQADAARQ
                     AALQALESDLGAISGPVFRIAPDFEVLTANDEATTLGRKPITGRDVLKVLPRADADAV
                     GDALAAVAAGARMAVRDTRPVKGRVMRWLVGARRDGAGVQLGFLAHGVDVSELAAAAV
                     PDVPEEVVVAPAEDSTALAEARAREESLQQALARVEAEREELRGGLAAADAQHAEQAA
                     ALRATLATVETEREELKAGLELARREAEEARNLADTAQRQVDEALAAAEDARRKEEKA
                     LRENGKLADALKATRSEQSAALAALAAAEQVPRQLRAELGEMRQGLRARIDELLDGVF
                     EPLLKDSAGQAPATGRSTTEKDKAS"
     gene            20128..21282
                     /locus_tag="PLS34_00555"
     CDS             20128..21282
                     /locus_tag="PLS34_00555"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HRP85993.1"
                     /translation="MQESMTIRRLVIVGSDEFATQAVDEAATAFGLLVRRVSRSDGAV
                     AAMTEFGPDAVILDPAALDRQAAEVLRAIAASGASLLLLGGPDDRATRVARELAAAED
                     VAVAGILAKPVSPEGLEVALRAMAGELVFSAADIAAAVMRGEMTAWYQLQLARSPSGW
                     RADGAEALARWQHPEHGAVLPDAFLPVAEAEGQIAAITDCVLLSSVQQLGVWHRAGMP
                     MRVGVNLSPALVTDPDFPDRLARLLLEYEVPARALVLEIPESGLALAPAGFLSMLARL
                     RIHGFGLALEHFGSGSSSLTDLYRTPFSELKIDRRLVSQLDEDEDARRLVRGIILLAH
                     ELGLETTAEAVETSATLELLHEAGCDRVQGFVISRPLPANEFQAVAGHWL"
     gene            21357..23468
                     /locus_tag="PLS34_00560"
     CDS             21357..23468
                     /locus_tag="PLS34_00560"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRP85994.1"
                     /translation="MSKVLKIILGLFGLFIALVLAAVIIIPMVVDPNDYRDEIARAVE
                     EETGRSFAIEGEISLSVFPWLGLEVGRMRLGNPPGFGDGHFAEIGSAAVGAKLVPLLS
                     RRLEVSTLRLEGLRVNLVQLADGSSNWSDLADGRSDPVADSAPRAEGDQQFRLERIDG
                     LRLSDALLRFDDRRAGRVVEVAIPRLATGELSPGKAFPLEAEALARFDADGLTAGATL
                     GGIIELAGDSTAVTIRDLALGVELSGAEVPGGRQSGRLDVPSLSADLDAQTLAIPALT
                     LEAAGLRAAAEVAGTGILDAPALSGRFQLQPFSPRALLRQLGEPAPETADPAVLDRAS
                     LEADFRAGAGQVALEGLRLQLDDTALAGAAAIGFGEVMRIRSELTLDAVDLDRYLPPE
                     AEQAGEQAPGEDVELAFDWLEKLDLDASFKAGTLKINGLTLTNLEGRAVARDGVLTLQ
                     PFGAALYGGEVRGTARLDARRTPATFSLEQSLSALQLLPFVKDLAGFEKLTGVAQLGA
                     RLSTTASSTADLMSGLNGELTFDVSDGALKGVNLWYEIQRANALARGRAAPQRSSPDT
                     DFRQLKGTAVIRDGMLVNQDLVGGLPFLALAGQGQVNLVESALDYRLNATVIREAIDE
                     ATGERSELAGATVPLRLRGSLDSPSVSVDLGGVVRERAQQEALQRLGIEPEEGKTAEE
                     ELKERAGDKARRLLDRLRKDD"
     gene            23489..24094
                     /locus_tag="PLS34_00565"
     CDS             23489..24094
                     /locus_tag="PLS34_00565"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRP85995.1"
                     /translation="MRRPRPGFQQPRPAATGISALVLAGALCLPAGSALGFELVSATT
                     SREKGEYRVRIEAVFKVPPAKLLAVLTDYDRIHELHPQITESRSLGPVGPATEEVYSR
                     FEACVLLFCRTVRRVEHIRMDGGSLFARDVPGRGSFSSGVTEWHFTPQGSGSRLRYEA
                     RFVPAFLVAPIIGPAVLARSAEQMAVETMAEAERRAEQADD"
     gene            24087..25148
                     /gene="mutY"
                     /locus_tag="PLS34_00570"
     CDS             24087..25148
                     /gene="mutY"
                     /locus_tag="PLS34_00570"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRP85996.1"
                     /translation="MTEGFAAELLRWYDRAGRHDLPWQSEPSPYRVWVSEIMLQQTQV
                     ATVIPYFRRFMARFPDVATLAAAPRDEVLHHWSGLGYYARARNLHEAAQRIVARHAGR
                     FPESLAEARALPGVGRSTAAAILALALGQRHAILDGNVKRVLARHHAVPGWPGAAKVE
                     RQLWALAEHYTPAARVRDYTQAIMDLGATVCRRSRPACGDCPVAAGCEARRLGRQAEY
                     PAPRPRRTRPLRHARMLLLNDADGALLLEQRPPQGIWGGLWCLPELGDESPLEWGLRA
                     LGTLIETAEPMAPIRHGFTHFELEIEAVPARVAAVPAALMETDRWVWYNARSPLKLGL
                     AAVVRRLIEARAERRVAAK"
     gene            25159..25434
                     /locus_tag="PLS34_00575"
     CDS             25159..25434
                     /locus_tag="PLS34_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HRP85997.1"
                     /translation="MTRTVQCVLLRREAEGLDRPPYPGELGQRIYEQVSREAWQRWLG
                     HQTMLINEYRLVVIEPEAREFLRTQMEQFFFGDGAVQPEGYVPPDQD"
     gene            complement(25448..>26112)
                     /locus_tag="PLS34_00580"
     CDS             complement(25448..>26112)
                     /locus_tag="PLS34_00580"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HRP85998.1"
                     /translation="AYVPVEISREHLQVAAARIAADFPELEVLPVTADFTEPFDLPEP
                     KRSRVRRNVAFFPGSTIGNFPRAMAADLLRATCQEVGPGGAMLIGVDLVKHPAILERA
                     YNDAAGVTAQFNLNLLVRLNRELGADFDIGGFRHQAVWDAARERIEMRLVSMRPQRVH
                     LAGRTFDFAAGEILVTEYSHKYTPDGFRSLAEASGFHVARIWTDAEQLFSVQLLEVAP
                     AA"
     assembly_gap    26113..26122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26123..26428)
                     /locus_tag="PLS34_00585"
     CDS             complement(<26123..26428)
                     /locus_tag="PLS34_00585"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HRP85999.1"
                     /translation="MENLRGVADFSPDDEVMLRDVLHGLALPARNIPSLYFYDQRGSE
                     LFDAITALPEYYPTRTEIGILEAHGGEMATALGPGVRLIELGSGSAVKTELLLARLEA
                     "
     gene            complement(26428..>27465)
                     /gene="egtB"
                     /locus_tag="PLS34_00590"
     CDS             complement(26428..>27465)
                     /gene="egtB"
                     /locus_tag="PLS34_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HRP86000.1"
                     /translation="ERFEYLFNSYYFTVGQMHLRPRRGLITRPSLAEVLDYRAHVDAA
                     VARLLERERGNPELEFLVTLGMHHEQQHQELMLTDIKHLLAQNPLLPAYVERPAPVAE
                     VAAPLRWIRGRAGPVEIGHAGEDFAFDNETPRHRVLLGEHSLASRLVTNAEYREFIDD
                     GGYSEPALWLSDGWSSVVAEGWRRPLYWQDDLAQEFTLAGLEPVQPDAPVTHLSYYEA
                     DAFARWAGARLPTEFEWEAAATGRPLDGNFAGTGAFHPRPAAHAEGLQQLFGDAWEWT
                     ASPYAPYPGFRPLAGSLGEYNGKFMCSQMVLRGGSCATPVGHVRPSYRNFFYPGQRWQ
                     FSGLRLARDEV"
BASE COUNT         4193 a         9357 c         9663 g         4242 t
ORIGIN      
        1 aattcccagg taacgacttt catgcgggcc atggtgccct aacgtggtca tgccgtgcag
       61 ccgtgaattc cgaagtgggc cgttccccgg gcgttccaat ggcttgacat cgggcccggc
      121 ttgtatttag atagttatca aaatatctaa atgactgggt ggcggggtga cggactcgtt
      181 gttcaaggcg ctggcggacc caacgcggcg cgaggtgctg aaacgcttgc agcgcggccc
      241 ggcgaccgcc ggggagctgg ctgaagagtt tccgcaggcg aaggcgaccc tgtcgcatca
      301 cttcaacgtg ctgaaggccg ccggcctggt gcgggcggag cgaatgggcc agcacgtggt
      361 ttattccctg aacacctcgg tgttcgagga gctggcgcga atgatggccg agctgttcaa
      421 accggagagg aaggcaaaat gagtgcgcga ctgacgcaat ggatttccct cgggctcgtg
      481 ctggccgcgc tggcggtctc tgcggccctc tatggcgacc tgccgtcgca ggtggccacg
      541 cacttcaatg ccgccgggga gccggatggg tggtcggacc gtccaacggc cgcgctgatg
      601 atgccggcga tcatggccgg cctgtggctg atgttgtggg tgctgccgaa aatctcgccg
      661 caggggtggc gtgtcgagcc cttcgcggac gtctggtccc gctgccagct cgccatcatc
      721 gccttcatgc tgctggtgca cgtcggcgtg ctcggccatg cagtcggctg gtggggcgcg
      781 gacctggggg gcatggtgat gatcggcgtg ggcctcctgt tcgccaccct cggcaactac
      841 ctcggcaaga ccacgcggaa tttctttctc gggataagaa cgccctggac gctggcatcg
      901 gacgaagtgt ggcgacgcac tcaccgcctc ggcggctggc tgatggtcgg agcgggtgtg
      961 gcgctgatgg tgatgggatt caccggcgtg cacgagctgg cgatcgccgt ggtgatcggc
     1021 gtcgccgtgc tggtgccggc gatctactct ttcttcgcct atcgctccat cgaaggcttc
     1081 aagccgccac cggaccatta gaactccgcc gccagtcgca gccacagggt ccgtcctggc
     1141 tcgaagattc tgcggctgct tgcgccgaga tcggcgctgc cccgctggat gtgctccgca
     1201 taggcacgat ccaggatgtt gtcgacgccg gcggtggccg tcagtccctg gccgaagcga
     1261 tagccgccat agccggacaa taccgcaaag cccggcgatt ccccggagtc caggctgtag
     1321 atcgtgccgt atcccgcatg gatgcggtcc tggcgcgcca ctgccctgaa cagcagtccg
     1381 ccgaagtagc gctccttctc gtagtccagg ctcagcgtgc cctgcagcgg cggcgtctgg
     1441 gcgagcggga cgccgtcggt gtcgttctcg ctgcgcaccc aggccgcggt gacggtggtg
     1501 ctgaagccgg gtcgcaggcg atagttgagg tcggcctcga ggccgctggt cttggcgtcc
     1561 acgttgcgtg cttcgaccgc ctcgagcccg ggcgccacga tcaggatgta atcgtcgatc
     1621 cggccggcga atacggccag gttcgccgtg accttgtcgc tgcgcaggct gacgccgagg
     1681 tcgagttggg tgagggtctc gttgtcgagc ccgaagacgc gccgcatctc ccagaaatcg
     1741 ggggcacgct cggcgcggcc caggccggcg tacagcgtga ccggccgacc ggagagatcg
     1801 cggctcaggc gcatgaacgc gctctgctgg tcgctcgtgc ggctggtgtt gggcgcggcg
     1861 ccgccgaaat tgatgccgtc cagcgcggtc gacttccgtc ggtcggcccg cacgccggcg
     1921 ttgaagcgtg aatgctcgcc cagcgcgcgt tccagctcga cgaacaggcc ggagtcggtg
     1981 aactcggcgt tgtcctcgcg cggcacattg cgatagttga atgcggccgg ccccatgagc
     2041 tggttcgacc cgtgctcgtt ctcggcccag tcgacgccgg cgacgatttc gagcgccggg
     2101 ctgacctgga actcgccggg tagccgcccg cccaccgtgc ggcggtccgg gtagctcacc
     2161 atcggctgca tcggcggcac gcgcagcgtg tagttgtcca tcacgtgatc gacgtagttg
     2221 tagtagagca atgcctcgag gcgggtgaac cacggcgcga gatccctgcg gtcgaagctc
     2281 agcgtatagc cggtgcggtc gaacacaacg ccgtccatgc cgcggtcgtc gtaggccgct
     2341 tcgccgtcgc tgcgttcggc ggcgaactcg atccgtgtca gctcatccgg cgtccagccg
     2401 agcatgccgg tggtgctcca gcggtggtac tgcgagtgca cggtgcgccc cgcgccgtcc
     2461 tcgtagtcgt cctgggtgga gagcgtgccg atcatgcgca cgtagccgag ccggtctccg
     2521 gccgtcacgt cgccgacgaa gtcccgccgg tcatggctcc cggcgatcag gctgccgaag
     2581 cccgtgacgg tgcgttcagt gaaacgcgtc ggttcgcgat cgaagcgcac gatgcccgcg
     2641 atcgtcgcgc cgtagcgcac ggattgcggt cccttgagga cctcgatccg gtcgtaggac
     2701 tccgggaaca cgtaggccgt cggcgggtcc atgcgcccgc cgcagccacc gagcacatgc
     2761 gtgtcgtcga gcagtatgtt cagccgcgag ccacccaggc cgcgcagctc cgggtcgccg
     2821 ctggtgccgc ccttgcggct gacggtgaag cccgggatgg acttgaggta ggcgccgccg
     2881 tcgtgggcgg gcagcggcag gcgcggttgg cgcgggtcgg tgacgatggt gtagggatcc
     2941 cgcatgggga cggcggtgac gacgaccggc tcgagttgcg cgtagtctgc cgcagccgtg
     3001 gcctcgggcg gctgtgactc ggtggcggcc agggcagggt cggccgcttc agttgcggcc
     3061 cacgtggtgg tgtgcagggc ggccagcagc aggcccccca tcaaagggat cttcatggcg
     3121 taggtacctc aggcagtgca gatcgacgac cgtcacatgc gtccggtcgc ggcgcgcagg
     3181 cggccgagcc ggtgtcggcc gggcgggcgg gccacggggt gtcagctgag gaccgggatg
     3241 ggcggtgccc gtgcgggcgg gacgatcagg ctcgggcggg agatgggcgg gagtgcgatc
     3301 tccggcacgg ccgcgggcgc cttgtcgagt gcaccggtgg gcgaagtgaa cgacgatgcg
     3361 gccagcgggg agagcagcag cgaccacgga caggtcgctg tcgcgccgtc gtcctgatcc
     3421 tcatcctgga gcagctcccc ggtggcgggg tcggccagga ccgtgcgcgg tccgtgcagg
     3481 gtgcacagct cgaccagttc gcccttcgcc ggcatgaagc cgtccggcac cagggcgcgc
     3541 aggaagagcg ccaggagcag gatcgaattc agcaggggtt ggcgtagcca gggcttcacg
     3601 cgtacatacc gccgcaaaag atgcctcgaa tatgcattaa tacccagcgc ctgtccaccc
     3661 cctggtcgga cccgcgcagg taaataattc taggcggaaa atccctgaaa gtggctctgc
     3721 acagcgcctt agagcaccga tttcgcggcg aaatcgcagt gctaagcgtc ctggctggcg
     3781 accccgaatt cgttagaacg tcgatctggg ggccgaaacg cgcagctaac gtcctaagca
     3841 gccgggaaat caggcgaaaa gtcttctaaa tcatgtggtt ttgagggtcc gacccgcggt
     3901 gtcgcgcaaa atttcgcgcg cagcgttgcg gccgggcagg cccgtcacgc cgccgcccgg
     3961 gtgggtgccg gagccgcaca ggtagaggcc gggcaacgct ccacgatagt cggcctggcc
     4021 gagcaggggc cgtgcgctga acaactggtc caggcccagg gcgccatgga agatgtcgcc
     4081 accgagcagg ccgaactcgc gctccaggtc gagcggcgag agaatgcgcc ggccgagaac
     4141 gctggcggaa aagttgggtg catgcctgtt caccgtctca atggcgagat cggcaaaagt
     4201 ctcgcggtgc gcatcccagc ccccgtcggg ctccggattc acgtgctggc agaacaggct
     4261 ggcgacatgc atgcccggcg gcgcgagcga ctcgtccagg gccgacgaga tcaccagctc
     4321 gaccaccggc tcgcgcgccc agcccgggtt gtgttcccgc gaccgcgcat cgaaccaggc
     4381 gcgctcgaaa tagccgagtg aatcaccgac caggatcccg ctggcatgat gcggctgcag
     4441 ctgcgtgccc ggcgcgcacc ggaaatccgg cagctcagcc agcgcgacgt tcatccggaa
     4501 cgtgccggag gcgcagcgat agcggccgaa tcgcgccagc agctccgggt ccagctccgc
     4561 cggatcgaac agccgcgtat acagcagctt agggttgacc cctgcgatca cccgccgcgc
     4621 ccggatctcg cggccgtcgg ccagggctac gccgaccgcg cgtccgccgc gcaccaggac
     4681 acgctcgacg gcggcgtccg tgctgatctc cacgccgcgc gcccgcgcct cggtcgcgat
     4741 cgcctcgctg atggcgccca tgccgccgac ggcgtgtccc cacgcgcccg gtttgccgtt
     4801 gacctcgcca aacacgtggt gcagcagcac atacgccgag cccggcgcat aggggctggc
     4861 gaagttgccg acgatcgagt cccagcccag cacggccttg agcggatcgc actcgaacca
     4921 gtcgtccagc agttcgccgg cgctgcgcgt gaacagctcc agcaggtcgc ggcggccgga
     4981 catgtcgagc cccttgagcc gccgggccac cgcccacgaa gccagccagt cggccagcac
     5041 gaagcgatcg gtcacgttcg gcggcgtctg ctcggccagc gcccgcagca ccacgaccac
     5101 acgctccagc atggcgaacc attccggcag ccgctccgcg tcccgcctgg agaagcggcc
     5161 cacctcggcc tgcgtcgacg caccgccgaa gcgaaacgcg ctcccgtcgc gaagcggcag
     5221 gaagttcgcc agcggccgtg cgacgatccg caggccatgc tccgcgagcc gcagctccgc
     5281 gatgatgcgc gggttgagca ggctcaccgt gtagctgcag ctggagttgc ggaagcccgg
     5341 gtggaactcc tcggtgaccg cggcgccgcc gaccgtcggg cgacgctcca gcacccggac
     5401 cttcaggccg gcgccggcca ggtaggccgc gcacacgagg ccgttgtggc caccgccgat
     5461 gacgagcgcg tcgaggtctt gcggctttgc catgcgttgc acgcgccttg cccgaggatg
     5521 cacgcactct agcggcccgc aggcggaaca ccaacctccc acctgcgtcc aatccggcag
     5581 ggcgccggga gagggcgtat ccgccaggct tctagcctag aataggcaca tgatcgcccg
     5641 acagggcagc gtgccgggcg gagaccggat ccggaggttg aattccatga aaaaaggctt
     5701 cgtgattggc ttgctggcgg tggtgttggt cgcctgtgcg accagcccga cgggccgcag
     5761 gcagctcatg ctggtctccc ccgaccaggc catctcggcc tcgcgcaccg cctatgtcca
     5821 gcagatgcgt gccttcgacg aaaagggcct gatcagcacc gacaaggcgc tggtcgcccg
     5881 gatcaaccag atcaccggca ggctggtcta ccaggcgatc cagatgtacc cggccaccgc
     5941 cgactgggaa tggagcatgg aggtcatcga cgagccggag acggtcaacg cctgggccat
     6001 ggccggcggg cgcatggcca tttacaccgg gctgatcgag aagaccaagg ccacggacga
     6061 cgagatcgca caggtcatcg gccatgagat cgcccatgcg ctcgccaacc acaccgccga
     6121 gcgcatgtcc gtggtcatgg cgacccagct cggcgtgctc gcggtcggcg tgtcgacccg
     6181 cgatgatcgc gccatggcgg gcgcggcggc cgcgtcactg ctggcgatca acctgcccaa
     6241 cagccgtact gcggagaccg aggcggaccg cataggcatc gagctcgcgg cgcgtgccgg
     6301 ctatcacccg gacgccgcgg catcactgtg gaagaagatg gccgaagtcg gcggcggcgg
     6361 tccgccgcag ttcctcagca cgcaccctgc cccgggtaac cgccagcaga ctctggcccg
     6421 gctggcgccg cagatgatgc agtattacga ggcgcccggc gaacgcccgg tacacccgat
     6481 gcgccccggc cgctactgac ccgttccacc tccgccccat cctgcgccgg ccgcggggcg
     6541 gcacggcgcc gtggtcgcgc ctagccgagc cgcgcggcga ggtagcggtg acaatccgcc
     6601 aggctggcga gccgcggata gtccgactcc gggatgtcga tgcccagcga ccggctcaag
     6661 gcgatggcga aattgagcgt gtccatcgaa tcgaagtcgg cctggtcccg aatcagcagc
     6721 tcagggtcga gttgatccag ctccaggtcc ggcgccacgc tcccgagcgc ggcgcgcatc
     6781 acctgctcga tatcgacggt attcatagcg acccgggctc ctgcatcaat cggctgatcc
     6841 ggtcgagaaa caatgcgccg cggtgcccgt cgctggtccg gtggtcggcg gccagcgtgg
     6901 tgcgaaggag ccagcgcggc tcgacccgcc cgtccgctac ccagggccgc tgcaccggcg
     6961 taccgacgcc gacgatcgcc acctgcggcg cgtagatgac cgggaagact tcctccacac
     7021 cctgctcgcc gaggctgctg acggtgatgg tgccgccggc gagttccgca gcggtcagcc
     7081 ggccggcgcg ggcgcgcgcg accagggcac ggaaagccgc catgagctcg ggcaggcttc
     7141 gctggtcggc gtcgcgcagg gccggcgcca ccagtccgcc gcggcgcaag gaaatcacgc
     7201 tgccgacatg gatgccgtcg ccctgcacga aagccccgtc gcgaaagtgg ccgttgaatt
     7261 ccgggtagga tttcgccgcg agcgccaccg cgcggatcac cagcaccgcg agcagcaagc
     7321 gctcctccac cgggcgcgcg gcgttccact cgtccagcca cttgttcgcc ggggcaaggt
     7381 cgatccagtg gccgagatag tagtgcggaa tctccctttt cgacctggtc atcgcggcgg
     7441 cgatgacggc gcgcatgtca gctggtgatg cgggtgtcat gctcgcaggc gactcgcttg
     7501 cggtcgccgc aggctcggcg ctggtccgcg gcgcgttcgc tgcgctacgc gcgcgccccg
     7561 cgcccgctgc ctcgacgtcg gccagcgtta tggcgccctc cggcccgctg ccatcgatcc
     7621 cctcgagcgc gacacccagt tcccgggccc tgcgccgtgc cgcgggggaa gcgcgcagct
     7681 gcggcgccgc gccacgcagc ctggcaagcg gctgtcccac cgggactttc gcgccttcct
     7741 cgaccaacag cgtctcgatt tcgccgtcct cgaagatctc gacgtcgatg gcgcccttgg
     7801 ccgtttccac cacggccacg acctggccgc gcctgacggc atcgccgggt ttgacccgcc
     7861 actcgaccag cgtgccctcg tccatgtcgg cgcccagcga gggcatcagg aagtcagcca
     7921 ccggtcactc tcctcgctgc ggccacgatc ttgtccacgc tcggcagcgc ggcctgctcg
     7981 aggtgggcgg gataggggat cggcacttcc tccgtgccta cccgcgcgat cggcgcatcc
     8041 agttcataga agcagtgctc caccaaccgc gcgacgattt ccgccgacaa gcccatggtc
     8101 cgccatcctt cgtccacgac cacggcgcgg cgggtgcggg cgatggaggc cgtcagcgtc
     8161 tcgtcgtcga gcggccgcag gctacgcaga tcgatcacct cggtatcgat gccgtcggca
     8221 gacagccgct cggccgcgtc gagcgatttc ctcaggctgc cgccgtaggt gatcaacgtg
     8281 aggtccgtac cgacgcggcg caccgccgcc cgctcgaggt ccacggcgcc ggcgtcctcc
     8341 tcgagctcgc cctccatgtt gtacaagccg atgtgctcga agatgatcac cgggtcaggg
     8401 tccgccaggg cggcgggcag catgcctctc gcatcttcca gcgtcgccgg cgcgaccacc
     8461 cgaagcccgg gaatgtgcgc gtaccagcac tcgaggctgt gcgagtgctg ggcggcgagc
     8521 tggcgcccgg cgccggtggc catcctgatg accagcggca cgctgaattg cccgcccgac
     8581 atgtgacgaa gggtcgcggc cgtgttgagg atctggtcca gcgcgagcag gctgaagttc
     8641 acggtcatga tctcgacgat cggtcgcatg ccgccgagcg cggcgccgat gcccgcgccg
     8701 gtgaagccgg attccgacag tggcgcgtcg aggatccgcc gctcgccgaa ttcctcttgc
     8761 aggcccatgc tgactccgaa gctgccgccg tagcgcccga catcctcgcc aaccaggaac
     8821 acacgctcat cggcatgcat ggccgcgcgg atcgcgccgc gcacggcctc ccggtaggtg
     8881 gttttcacgc ggctcacggt gcacgctccg cggtcacgtg gcgcgccagt gtctcgacgg
     8941 gctcccaggt gccggcctcg gcgaaggcca ccgcctgctc gacttcgccg gcaacctcgc
     9001 gctcgattac ggccacttcc tcgtcgctgg ccagcccgtc ggccagcagc cgccggcgca
     9061 gcgcaagaat cgggcagcgc tgcttccagc gctcgacttc ttccctgctg cgatagagct
     9121 gcgcgtcgaa catcgagtgg gcgcggaatc ggtaggtgcg gcactcgatg aagcgcggcc
     9181 gctgatcccg gcggatggcc gccgccgcgg cgccggccgc cgcctcgacc gcgagcacat
     9241 ccatgccgtc cacgacggac gactcgactc catagctggc ggcacgcgca tggatgtcgg
     9301 tcacggcctg ggatcgtgcc agcgccgtgc ccatcgcgta gaggttgttc tcgcagatga
     9361 acagcaccgg cagttcccaa agcgcggcca agttgagcga ctcgtgaaac tcgccctcgg
     9421 cggctgcgcc ctcgccgaag aagcaggcgc tcacgcgcgc gcgttcctgc atgcggtcgg
     9481 cgagcgccag ccccaccgcc atcggcagcc cgccggcgac aattgcctgc cccccgtaga
     9541 accggtgctc cgcgtcgaag atgtgcatcg ccccgccgcg cccgccgctc gatccttcca
     9601 ccttgccgaa cagctcggcc atcaccgcgc ccatcggcaa cccggctgcg agggcatgcc
     9661 catgctcccg ataggtggcg atgacggcgt cgccgggccg caggccgccc atgacgccga
     9721 cagccacggc ctcctcgccg ttgtaaaggt gcaggaagcc gcgaatcttc atcgcggtgt
     9781 acagttcggc ggcgcggtcc tcgaagcgac gaatgcgctc catggcccgc aacaggtcaa
     9841 gcccgtgctg cttgcagagc gcgatgggcg ccggatcact catcggcccg actcgagggt
     9901 ggacgtgtcg ccctcggcca ggccgagttc gcgagccttg agcagtcgcc gcatgatctt
     9961 gccgctgcgg gtgcgaggta gttcgtcacg gaactcgacc tgccgcggcg ccaatgccgc
    10021 gccgaggcgc ttgcgggtga atccgatcag cgccagcgca aggtgatcgt cgcctgcgac
    10081 gccctgcttg agagtgacga acgctttgac cgtctcgccg gccacggggt cgggcaggcc
    10141 gatcaccccg gcctcggcga cggccgggtg ctccatgagc gcgctctcca cctcgaaggg
    10201 gctgatgaga tgcccggcgg acttgatgac gtcgtccgcg cggcctacga accacagcca
    10261 gccgtcggca tcacaccgcg ccatgtcacc ggtgagatac cagccgtcgg cgaaggcgcg
    10321 ccggtagcgc gcctcgtcgt tgaggtagcc gcggaacatg gaaggccagc cggccctgag
    10381 agcgagctcg ccctcgattc cgggagccgt aatgatctcg atggcgggcg ggtcgtcatc
    10441 gtccgcccgc gataccctga cgatggcggc ctccacgccg ggaacgggcc gccccatgga
    10501 gccgggccgg acccgtgacg cgcggaagtt ggcgatcatg atggcgccgg tctcggtctg
    10561 ccaccaggtg tcgtgaatgg gcctgccgaa gatgtcccga ccccagcgca cggcctcggg
    10621 attgagcggt tcgccgacgc tggcaatgtg ccggagccgc gagtagcagt acgacgtggc
    10681 cagctcgttg ccctggcgca tgagcatgcg aatcgcggtg ggcgccgtgt accagacgtt
    10741 gacaccctcc tcctgcagga tcctgtacca ccgggccgcg tcaaactcct cgcgatcgac
    10801 gatcatgctt gcgccgttga ccagcggcgc gatggcaccg taggagatgc cggtgaccca
    10861 gcccggatcc gccgtacacc agtacacatc gccggcgtgc aggtcgaggg cgcagcgccc
    10921 ggtggcatga tgggccacca cggcccggtg ggcgtgcatc acgcccttcg gcttgccggt
    10981 ggtaccgctc gtgaagtgga gcaaggcgat agattccggg tccgtggcgg ggatctccca
    11041 gtcatcgctc gcggccgcca tcaattccgg gtagctgagc gtacccggcg gcagctcgcc
    11101 ggcagcagca tcgatcaaca ggacgtgctc gagcttcgac agggcgcccc tgatggggag
    11161 taccttgcgc tggtaaagcg agcgggtggt cacgagcacg cgaccgctgc caagttccag
    11221 gcgggaacgg accggctccg ggccgaaggc cgagaacagg gggcaataga cggcgcgcgc
    11281 cttcagggcg ccgaacatcg tcacgtagag ctcgggtgtg cggccggcca gcgagaacac
    11341 cgcgtcgccg ggggcgacgc cgagttcgtg caacacgttg gcgaagcgat ccgacagccg
    11401 ggccaggtcc gcgtagctga ggtcctcgcg accccagtcc cgcgcaatga atcgcatcgc
    11461 cagccgctcg ccgaggccct gggacacgaa gcggtcgagc gcctcgtgag cgatgttcaa
    11521 tcctccggcc ggcaagcctt ccagctgccg ctcggcctgt gcccagctga aggccgcaca
    11581 ggcgcttcga taatccgtca tgttgggggg cggttcgccg aaacgcacgt ggctgcggtg
    11641 gaatatttcg aacctcatga gcgcaccccg taccggccgg cgagcgaatg ggcatccctt
    11701 gcctgaagag tagtcgagcc gccggaaacg gccgtgagga aatatacgca gcaaagaaaa
    11761 ggcccccgga cttgcgtcgc gagggcctaa accaagagga gagtgtcggg agccgggacc
    11821 gcgaggatca agaccggccg gcagtgcctc cctggcgtac cggccggcgc cacatccgtg
    11881 tggtctcgac tgccgggatc atttatagga ttgctgcacc gcgatttcat tgaccggggt
    11941 caagttcgcg agcgaatgcg gaatgtccct agtaagcgct gcgggccgcg atgcgcacgg
    12001 ggcgagtgcc gcgcccggcg cgcgaccgcc ggccgggact actggcccgg gaccccggtc
    12061 gtgcccggcc ggaaccggta cacgtggtac gggccatgct cgcgaatatg tccgcgcgtc
    12121 ttcatgacca cctccggcag ggcgctgtcc gccaggtaga gccagccgtc cggcccgaac
    12181 gacagcccgt cggcccagcg gacccggggt gagcgcacta tggtggtgag ttcgccgccc
    12241 ggcgccatgc gcagcaccgc gccgtgctcc acgtcggtga tgtagacgtt gcccgacagg
    12301 tctgccgaca gcccgtcgct gagcggcttg cggccgaccg cctcgatgcg ctccgccagc
    12361 gccctggagt ccagtgcatc gtcgagcaat gcaccggtcg ggatgcggtg cagggtgtcg
    12421 tgggtcatgg cgccgaagta gagccactgg cccgcggggt ccagcgcgat gccgtccagc
    12481 ccgattttca tcggcacgaa gccgccgaag aatcgcatct cgcgcatcgg cgtcctaatg
    12541 atccagtcct gggctcgcag cgagggatga tcctcgaggg tccgccgcga gcggcgcgtc
    12601 agcgtgtcgt acaccaccag cgccggcgac ttgcgccaga cgctggcgtc ggcgatgtag
    12661 accatgcggc cgtcgcggtc cacctgcagg tcctgcagga aggagccacg cggcgccacg
    12721 cggcgcggaa agacgtgctc gtgaacgatg cgatcgttgt ccaggtcgat cgccacgatg
    12781 cgcggccggc cgagcccgtg cgcggccgga tcgatcaccc acaggcgatt ctggcgatcg
    12841 atggcgaggc cgagcggcgt ctccagcacc gccgcctgca actcctcgtc cggaaacggc
    12901 tccgcgcgcc cgccgcgcca ctcgtagagg cgcggtccgt cggtgcgggc ctcgggatgc
    12961 accgtgaaga acaaccggcc ggtggcgctg accgcaacgt tgcccagcgg ctcgggatgg
    13021 gagagcaccg tctccaggcc gctctcggtc acagacggct ggccggccat ttcggggaac
    13081 ggcccgccgc cgccgaaatg caggcgcaag ccgccgatcg ccaggacgac gaggagggtg
    13141 gcgagaatga caatgcggaa aatggtgctc atggccggtc cggacttttg ccgcagagta
    13201 gcggccgccg ccgcagatgc gcatgaactg cgtcacaaag tgactgcaag taactgcgcg
    13261 tcaaggaaag aagccgggtc aggcggccga atgctcgagc gcgtcctgca cccggcgcag
    13321 caggatccgg tcgatcgccg gcttctggat gaagtccacc gccccggcgc ggattgcgcc
    13381 caccgccgtg gcgacgtcgg attcggcggc cagcaggatg gtgggtacct ggcgccctcg
    13441 ttcgcgcagc gtgtgcagca gctcgaggcc gctcatgccg ggcagctcca ggccggccac
    13501 cagcacccgc atcgacatgt cgtcgagcgc atccagcagc gcttcggctg actcgaaggc
    13561 gcggacgtcg tgctgaagat tgcccagcag ttgtgacagc gcggcccgct cccgggcatc
    13621 gccatccacg acgcagatcg tcgcctgctg tgcctcagcg ctgcttcccg agactggggc
    13681 catgtttttc cggaccgggc agggcatgcg gaacggtccg ccttgcccgg gctgacgtcc
    13741 tctgtgatgt tcttcttgtg gccctgcgag tattgaaact cacccgcggc tttgaaatac
    13801 gaacatctac ctaagtcgca agtcgcatgc cgggccgggg tcaggcgccg ctgtggttct
    13861 ccgccaccat caccatgcgc accaggtgcg cgagggaatt ggcctgcatc ttctccatca
    13921 ccctggcgcg gtgaatttcc acggtgcgtt ggctcacgcc caggtctccg gcgatgacct
    13981 tgttcgcctt gccgagcacc acctgcccca tgacctgctt ctcgcgggcc gtcaggctct
    14041 cgatgcgatc gagaatttcc aggcgctcga gttcgccttc acgttgctcg gccgccagct
    14101 tcatcgcgtc gttgatgcga tcgaccagct cctggtcctg gaagggcttt tgcaggaagt
    14161 ccacggcccc ctggcgcacc gcctccacgg ccatgggaat gtcgccatgt ccggtgatga
    14221 atacgatcgg gatgttgcag ccgatctccg caaggtgttt ctgcagctcg agcccggtca
    14281 tgcccggcat ccggatgtcc agcaccaggc agccgtaatc ccgcggatcg aaggccttga
    14341 ggaagtccct ggcgcctgcg tagcacctgg cgcggtagcc cgccgagcgc agcaaaagcc
    14401 ccagcgaatc gcgcaccgcc tcgtcgtcgt ccacgatgaa taccgttgcc tcgcttgacg
    14461 ccattattcc tcctccgggg ccgtgggaag cctgatcctg aagacgctgc ctttttctgt
    14521 gccggaatcg tatcgcaatg tgccgccgtg cgcctcaatg atggagcggc tcagtgacag
    14581 gccgagaccc atgccctcgg gcttggtcgt gaagaacggc tcgaacagct gctcccgcag
    14641 cccgtcggca acgccggggc cggtatcctc gacggccagc tcgacctcgt cttcgttgag
    14701 cgccacggtg cgaacgacca gccggcgtgt ctcggcctca acggcgttca tcgcctccac
    14761 ggcgttgcgc gtcaggttca gcagcacctg ctgcaactgg atgccgtcca cctggacctt
    14821 gggcaggtcg acctggaagg cgagctcgag ctccacttcg tgcgcgcggc aatccatctc
    14881 caccaagggc aggatttcct ggatcagctc gttggcgtcg cggtgccggc gatccggcgt
    14941 gtggcgtcgc acaaaagcgc gcagcctgtg aatcacgtcg cccgcacgcc gcgcctgcgc
    15001 ggtcacgcgg ccgaggactt gtccgagctc atccggctcc atgtcgccgg cggccagcag
    15061 tcgctggcac gcctgggtgt aattggcgat cgcggccagc ggctgattca gctcgtgcgc
    15121 gatgcctgcg gccatttcac ccagggtgcc gagtcgcgcg acgtgcgcga gccgttcgcg
    15181 ctgcttgcgg gcctccatct ccgcctgcac cacgtcggta cggtcgagaa tctggctgac
    15241 gatgagcaag ggcctgccgt cgtggtccga gatcaggctg agattcacca gcgcgtgtac
    15301 gatcgagccg tccttgtgca ggaagcgctt gtcgtacttg acggtgtcgc cgcccgtcag
    15361 cagcagctgg aagtggcgct gggtttcgcc gatatcttcc ggcggcgtga cgtcccggac
    15421 cgagcgcgac agcaattcac cctcggtgta gccgagcatg ttgcacaggg cctggttgac
    15481 cgacaggtag cggccgtcga tttcgtgtgc tgcggccagc gtcgccatgc ccaccggcgc
    15541 gcgcctgaat atgagccgca gttcctcctc ggcctggcgc agctgcgact ctgtctcgcg
    15601 ctgctcggtg acgtcctcgc cggcgctgag cacgccgttc acgcggccgc tgtcatcgcg
    15661 caggaactgg ttgcgccaga acacgcggcg gtgatggccg tcgcgggtcg tgatcggccc
    15721 ctccatcagg ctgaagcttt cctcgcggcc ggcgagtgcc tcctcgaaca gtatcctcga
    15781 gcgtcccgca aaccccggcg gctggcaggc ggcaaaaaag tccttgccga gcagctcgtg
    15841 ctccgggtag ccgaacagct cgcaggcctt gcggttgacg agggtgatgc ggccggagcg
    15901 gtccatcgcg atgatcatca cgttgaccag gtcgagatag cgctgcgccc ggtcgcgctc
    15961 gcggcgcaac gcttcttcag cgtggcggcg gaaggtctgg tcgaggatcg tgccctggat
    16021 gcgccgctcg ccgctcatcg ggttggtgac gatctggtag atggtctgga tgtggcgcac
    16081 ggtaccgtcg ggtcgcacga tccggtactc gagctcgccg ccgccggagg ttttcctggc
    16141 gtcttcggcc agccgcgcct cgacccggtc ccgatcctgc gggtgcgcga agcggcgcac
    16201 gaactcggcc gggccgagcg cctgcgtatc gggatcgagg ccgacgatcc tgaagaactc
    16261 gtccgaccag aaattcgacc tggactcggg ccagtcaccc tcgaagctgc cgacgtgcgc
    16321 cagcgcctgg gcggtcagca ggttcgcctc gacctggtgc agccgggcct cggccgcgat
    16381 gcgcgcgctg atgtcgcgga tgatgccgat gaagctcgtc gtcccttcgt cgccgacacg
    16441 gcccaccgag acctcggcgg ggaacacggt gccgtccttc ctgcacgcct cgacttcgcg
    16501 cggcgccagc atgaagcgtg cctcgccggt ctgctggtag cgttggatgt aaccgtcgtg
    16561 accggcacgg aacggcgtcg gcatcagcag cgcgacgttc ctgccgacga tctccgcggc
    16621 gctgtagccg aagacccgct cggcgccggg gttgaaggac tcgatgacgc cgtcgccgtt
    16681 gatcaggatg acggcgtcgg gcgtggcgtc cagcagcgcc gagagtcggt actcagcgcg
    16741 cggaatgtcc ttgcgtgtcg gactggccgt gggcgtatcg cggctgttca tcggcgtgac
    16801 ggggtctctc cggcgtggca accggcgccg cgggacgcat ggccggtcag caggtcgcgg
    16861 tctagaatac ctgctgggac agcccgaaag catcccgcga tctgcgggtt gcgctgccgc
    16921 gacgggggta gaccagcgcc atgaagaagt tcagcaggat cctcgtcggc atcgacccgc
    16981 acgacacttc gttgcacgcg gccgcgctgg gcgcccgcct ggcagccggc accggcgccg
    17041 cgctggaact gttcaccccg gtcttcaatt cgcagatctc gctggcccat ttcgagaacc
    17101 gccagagcct gcagcacgcc cgggacctcc tggtggcgcg acaccgggag cggctcgagg
    17161 cacacctggc cgggttgggc atcgacggca ccgtggagtg ccacgccgcg tgggatcacc
    17221 cgatcgagga ggcgctcgtt cggcgcgtgc tcgagcgtgg cgcggatttg ctggtggtgg
    17281 cgctgccggt cggggccggc ggcggccggc cgttgtcggc cgcgcaatgg cagctcgtcc
    17341 gccattgccc gtccgcggtg ctgttgacgc gcggcggcgc ctggcgtgac cggccgacca
    17401 tcgtggcggc ggtcgatccg tcgggccacc gcgggcgtca tgacgcgctc gacatccgca
    17461 tcctgcaggc agcagggcgg ctggccgacg gcgtcagcgg cacggtacac gcgttccatg
    17521 cctggcagaa aagcctgcgg gccctgatcg gcgaggtcga gctgccgctg gcgaccggca
    17581 cagcggaaac cggtgtcgag gaccgccatc gcgaagcggt ctacgcggtg ctggccgccg
    17641 ccgacgcctc gccggcgcgc gtcgccttcg tcgccggccg cccggagacg gccttgcccg
    17701 agtattgccg cgaggtggag gcggacgtgg tcgtcatggg cgcgatcgcg cgcaatccct
    17761 tcggtcggat actgctcggc agcacggccg agcgcgtgct ggaccggctg ccctgcgacc
    17821 tgctggtagt caagcccgag gccttcagga gcccggtttc ccgggagcgc tggccgctgg
    17881 acgaagacgg gatgacgatc ctgggggtgc ccgggatctg aaccggccac ctgagcaacg
    17941 actccggcgc aggatcgtgg taatttccgg gtgccgccaa taaaggatta cataacatcg
    18001 atggcccagt cgctgcgcat cctcctgatt gccgatgacc ccgagctgat ccgccggatc
    18061 gggcatggcc tgcaacgcca ggacagcggc ctgatcatga tcgagggtgc cgatacgctg
    18121 gcgacggctc gccggcggct cggcgccggc acgtatgacc tggccctggt cgacctgtca
    18181 ctgggacacg gggacggcct gcacctgttg tccgacctgc aggaaatcgc gccggagctg
    18241 ccgatcgtgg cgctggccgc aaacggcaac ggcccggatg cggcggcctg ccttgcgctg
    18301 ggtgcgcagg atcggctcgc ccctgaggcc atggattccg ccggcctgct cgatcggctg
    18361 cacagcgcgc gggcccgcgc ccaggccggg ctgcgcctgc gccggcgcag ccatcgcatc
    18421 gccgcgagcc tcggggcgac gggcgatctc gcctggcatt acacccatgg tgagggcgaa
    18481 gtctggctgg cggcggccaa cccggtggaa tggcagctgc ctgcgcccga gtgcagcgaa
    18541 tcgctggacg cgctgcgcgc acgcattcac ccggacgacc gcgagttggt actgcgccag
    18601 ctcgaggaag tggttctcag cagccagccc tggcagctgg atgcccgctt caaggtcgac
    18661 ggcggcgcct atcgctggtg catcctgcgc ggccgctcgc aactcgatgg tcgtggcgca
    18721 ctccagtatg cctccggggt gctgtccgac gcacagcgcc agcaaaaacg gctgcgcgag
    18781 gtggaacagg gccggcgatt cctgcgggcc gtgttcgact ccgcgcgggt gccgcaggcc
    18841 atcctggatt cctcggcggt gatcagcgac tgcaaccagg cctgggtcgc gctcgacgag
    18901 ccggcctgtc acgccggcag cgccttcggc ccgggacgcc ggttcatcga caagccggag
    18961 gacccggccg cctacgggga cctcgaccac gccgagctgg cgcggggcgt gcgccaggtg
    19021 ctcggcggag tcgcggacca gttcgtctgc gagtacggcg acagcgagcg gcgctggaga
    19081 atgacggtca cgccgttgct gaatcccggc atcgccgggg ccgtggtgcg ccacgaggag
    19141 gtcaccgaga cgcggcaggc ggatgcggcg cgacaggcgg cattgcaggc gctggagtcg
    19201 gacctcggcg ccatcagcgg gccggtattc cggatcgcac ccgatttcga ggtgctgacg
    19261 gccaacgacg aggcgacgac cctcgggcgc aagccgatta ccggtcgaga cgtgctcaag
    19321 gtcttgccgc gtgccgacgc ggacgcggtc ggcgacgccc tggcagcggt tgccgcaggg
    19381 gcgcgtatgg cggtacgcga cacgcggccg gtgaaaggcc gggtgatgcg ctggctggtc
    19441 ggcgcgcgtc gcgatggcgc gggcgtgcag ctcggcttcc tggcgcacgg cgtggacgtg
    19501 tcggagcttg cggccgccgc cgtgccggac gtcccggaag aggtcgtcgt agcgcccgcc
    19561 gaggactcca ctgcgctggc ggaggcccgc gccagggaag aatcgctgca gcaggcgctg
    19621 gcgcgcgtgg aggccgagcg cgaggaactg cggggcgggc tggctgccgc ggatgcgcaa
    19681 cacgcggagc aggcggcggc attgcgcgcg acgctcgcca ccgttgagac ggagcgcgag
    19741 gaactcaagg cgggtctcga actggcccgg cgtgaggccg aggaggcgcg caatcttgcc
    19801 gacacggcac agcggcaggt ggacgaggcc ctcgcggccg cggaggacgc ccgccgcaag
    19861 gaagagaagg cgctgcgcga aaacggcaag ctggcggacg ccctgaaggc gacccgcagc
    19921 gagcagagcg cggcgctggc cgccctggcc gccgccgagc aggtaccccg gcagctgcgg
    19981 gccgagctcg gggagatgcg ccagggactg cgggcgcgca tcgacgagtt gctcgacggt
    20041 gtgttcgagc ccctgttgaa ggactccgca ggccaggcgc cagccaccgg caggtccacc
    20101 acggaaaaag acaaagcgag ttgatacgtg caggaatcga tgaccatccg gcggctggtg
    20161 atcgtcgggt cggacgagtt tgcgacccag gcggttgacg aggcagcgac ggcgttcggg
    20221 ctgctggtgc ggcgcgtctc gcgcagcgac ggcgccgtcg ccgcaatgac ggagttcggt
    20281 ccggatgcgg tgattctcga tccagcggca ctggacaggc aggccgcgga ggtgctgcgc
    20341 gccatcgcgg cttcgggcgc gtcgctgctg ttgctcggcg gcccggatga tcgcgccacc
    20401 cgggtggcgc gcgagctcgc cgcggccgaa gacgttgcgg tcgcgggcat ccttgccaag
    20461 ccggtgtctc cggaagggct ggaggtcgcg ctgcgcgcca tggccggcga gctggtgttt
    20521 tcggctgccg acatcgctgc cgcagtgatg cgcggtgaaa tgaccgcctg gtaccagctg
    20581 cagctggcgc gcagcccctc gggctggcgg gcggatggcg cggaggcgct ggcccgctgg
    20641 cagcatcccg aacacggcgc ggtgctgccg gacgccttct tgccggtggc cgaggccgag
    20701 ggccagatcg cggcgatcac ggactgcgtg ctgctgagct ccgtgcagca gctcggcgta
    20761 tggcaccggg ccggcatgcc gatgcgcgtc ggcgtcaacc tctcgcctgc gctggtcacg
    20821 gaccctgatt ttccggatcg cctggcgcgc ctgctgctgg agtacgaggt gccggcgcgc
    20881 gcgctggtgc tggaaatacc cgagtccggc ctggcgctgg cgccggcggg cttcctgagc
    20941 atgctggcgc ggctgcggat ccacggcttc ggcctggcgc tggagcattt cggcagcggc
    21001 agctcctcgc tgaccgacct gtatcgcacg ccgttcagcg agctgaagat cgaccgacgc
    21061 ctggtcagcc agctggacga ggacgaggac gcccgccggc tggtgcgcgg catcatcctg
    21121 ctggcgcatg agctggggct ggagacgaca gccgaggcgg tcgagacgag cgcgacgctg
    21181 gagctgctgc acgaggcagg gtgcgaccgc gtgcagggtt tcgtcatcag ccgccccctt
    21241 ccggcgaacg agttccaggc cgttgcgggt cactggctct gagtagcgcg ccggcgcgga
    21301 ccctataatc gggtcaaagg gcgcccctcc ggcgccgccg accggagcct ctcctgatgt
    21361 ccaaagtcct gaaaatcatc ctcggcctgt tcgggctgtt catcgcgctg gtgctggctg
    21421 cggtcatcat cattccgatg gtggtcgacc cgaacgacta ccgggacgag attgcgcggg
    21481 cggtcgagga agagaccggc cgcagtttcg ccatcgaggg cgaaatctcc ctcagcgtgt
    21541 ttccctggct cggcctggag gtcggccgga tgcggctcgg caatccgccg ggcttcgggg
    21601 acggtcactt cgccgagatc ggctcagcgg ccgtcggcgc aaagctggtg ccgctgctgt
    21661 cgcggcgtct cgaggtcagc accctgcggc tggaaggcct gcgcgtgaac ctggtgcagc
    21721 tggcggacgg ctccagcaac tggagcgacc tggccgacgg caggagcgat ccggtcgccg
    21781 acagcgcacc gcgtgcggaa ggcgaccagc agtttcgcct ggagcgcatc gacggcctgc
    21841 gcctgagcga cgcgctcctg cgcttcgatg atcgtcgcgc ggggcgtgtc gtcgaggtcg
    21901 cgattcccag gctcgctacg ggcgagctct cgcccggcaa ggccttcccg ctggaggcgg
    21961 aagccctggc ccgcttcgac gcggacgggc tcacggccgg cgccacgctt ggcggcatca
    22021 tcgagctggc cggggactcc actgccgtca cgattcgcga cctggcgctg ggcgtcgaac
    22081 tcagcggtgc ggaggtgccc gggggcaggc agtccggacg gctcgacgtg ccgagcctca
    22141 gcgctgatct cgatgcccag acgctggcga tcccggcgct gaccctcgag gctgcggggc
    22201 tgcgtgccgc ggcggaggtt gccggcaccg gcatcctgga tgcgccggcc ctcagcggac
    22261 gcttccagct gcagccgttc tcgccgcgtg ctctgctccg gcagctcggc gagccggcgc
    22321 cggagaccgc ggacccggcg gtgctcgacc gggcctcgct ggaggcggac tttcgcgccg
    22381 gagccgggca ggtcgcgctc gaggggctgc gcctgcagct cgatgacaca gccctggccg
    22441 gcgccgccgc catcggtttc ggcgaggtca tgcggattcg ctctgaattg acgctcgatg
    22501 ccgtcgatct tgaccgctac ctgccgccgg aggccgagca agcgggcgaa caggcgcccg
    22561 gcgaggacgt cgagctggcc ttcgactggc tcgagaagct ggacctcgac gcgtccttca
    22621 aggcgggcac gctgaagatc aacggcctga cgctcactaa cctcgagggc cgtgccgtgg
    22681 cccgggacgg cgtgctgacg ctgcagccct tcggcgcagc gctctacggc ggcgaagtcc
    22741 gggggacggc gcgtctggat gcacgccgca cgccggccac cttcagcctg gagcagtcgc
    22801 tgtcggcgct gcagctgctg ccgttcgtca aggacctggc cggcttcgag aagctcaccg
    22861 gggtcgcgca actcggcgcc aggctgagca ctaccgcgag cagcaccgcg gacctgatgt
    22921 ccgggctcaa cggggagctg accttcgacg tatccgacgg cgcgctcaag ggcgtgaacc
    22981 tctggtacga gatccagcgc gccaacgcgc tggcgcgggg gcgggcggcg ccgcaacgca
    23041 gctcgccgga caccgatttt cgccagctca agggcacggc cgtgatccgc gacggcatgc
    23101 tggtcaacca ggacctggtc ggcgggctgc cgtttctcgc cctggcgggc cagggccagg
    23161 tcaacctggt cgaatcggcg ctcgattacc ggctcaacgc cacggtgatc cgcgaggcca
    23221 tcgacgaggc taccggggag cggagcgaac tggccggggc cacggtgccg ttgcgcctga
    23281 ggggcagcct cgactccccg tcggtcagcg tcgatctcgg cggcgtggtt cgagagcgcg
    23341 cccagcagga ggcgctgcag cgcctcggca tcgagccgga ggaaggcaag acggccgagg
    23401 aggaactgaa ggagcgtgcc ggcgacaagg cgcgtcgcct gctggaccgt ctgcgcaagg
    23461 acgactgacg gccttgcctc gaccggtcat gcgtcggccc cgacccggtt ttcagcagcc
    23521 gcggcccgcc gccacgggca tctcggcgct cgtgctcgcg ggcgcgctct gcctgcccgc
    23581 cggcagcgcg ctcggcttcg aactcgtctc cgcgaccacc agtcgcgaga agggcgagta
    23641 ccgggttcgc atcgaggcgg tgttcaaggt cccgccggcg aagctgctgg ccgtgctgac
    23701 cgactacgac cgcatacacg aactgcatcc gcagatcacc gagagtcgct cgctgggccc
    23761 ggtgggaccg gccacggagg aggtctattc gcgtttcgag gcctgcgttt tgctgttctg
    23821 ccgcacggtg cgtcgcgtcg agcacattcg catggatggc ggctcgttgt tcgcccggga
    23881 cgtcccgggt cgcggctcgt tcagcagcgg ggtcacggag tggcatttca cgccgcaggg
    23941 cagcgggtcc cgcctgcgct acgaggcgcg tttcgtgccc gccttcctgg tggctcccat
    24001 catcggcccg gcggtgctgg cgcgttccgc ggagcagatg gcggtggaga cgatggccga
    24061 ggccgagcgg cgcgccgagc aggccgatga ctgagggctt cgccgccgag ctgctgcgct
    24121 ggtacgaccg ggccggccgc cacgacctgc cgtggcagtc cgagccctcg ccttaccgcg
    24181 tatgggtctc ggagatcatg ctgcagcaga cccaggtggc gacggtgatc ccgtatttcc
    24241 gtcgcttcat ggcgcggttc ccggacgtcg ccacgctcgc ggccgcgccc cgtgacgagg
    24301 tgttgcacca ctggtcggga ctcggctact acgcccgggc gcgcaacctg cacgaggccg
    24361 cgcaacggat cgtcgcgcgg cacgcggggc gcttcccgga gtcccttgcc gaggcccgtg
    24421 cgctgccggg cgtcggccgc tccaccgccg ctgcgattct cgccctggcg ctcggccagc
    24481 ggcatgccat tctcgacggc aacgtcaagc gcgtgctggc gcgccaccac gccgtgcccg
    24541 gctggccggg ggcggcaaag gtcgagcggc agctttgggc gctggccgag cactacacgc
    24601 cggcggcgcg ggtgcgcgac tacacgcagg cgatcatgga cctgggtgcg acggtctgcc
    24661 ggcgcagccg cccggcctgc ggggactgcc cggtagcggc cggctgcgag gcccgcaggc
    24721 tggggcggca ggcggagtat ccggcgccgc ggccaaggcg gacacggccc ctgcgccacg
    24781 cgcgcatgct gttgctgaac gatgcggacg gcgccctgct gctcgagcag cggccgccgc
    24841 agggcatctg gggcgggctg tggtgcctgc ccgaactggg cgatgaatca cccctggagt
    24901 ggggcctgcg cgcgctcggg acgctgatcg agaccgccga accgatggcg cccatacgtc
    24961 acggcttcac gcatttcgag ctggaaatcg aggccgtgcc ggcgcgggtc gccgcagttc
    25021 ccgcagcatt gatggagacc gaccggtggg tctggtataa cgcccggtct ccgctcaagc
    25081 tcggtctcgc ggcggtcgtg cgccggctga tcgaggcgcg ggcggaacgc cgcgtcgccg
    25141 cgaaatgaag gaaccgtcat gaccagaacc gttcaatgcg tgctgctccg tcgcgaggcc
    25201 gaaggcctcg accgccctcc gtacccaggc gaactcggac agcgcattta cgagcaggtt
    25261 tcgagggaag cgtggcagcg ctggctcggc caccagacca tgctgatcaa cgagtaccgc
    25321 ctggtcgtca ttgagcccga ggcgcgcgag ttcctgcgca cccagatgga gcagttcttt
    25381 ttcggcgacg gcgccgtgca acccgagggc tacgtgccgc ccgaccagga ttgagcggtg
    25441 ccgccggcta ggctgccggc gcaacctcga gcagctgcac cgagaacagt tgttcagcgt
    25501 cggtccagat tcttgcgaca tggaagccgc tggcttcggc aagcgatcgg aacccgtccg
    25561 gcgtgtactt gtgcgagtac tcggtgacca ggatttcgcc ggcggcaaag tcgaaggtcc
    25621 gcccggccag gtgcacgcgc tgcggccgca tgctgaccag ccgcatctcg atgcgctccc
    25681 ttgcggcatc ccacacggcc tggtggcgaa accccccgat gtcgaagtcc gcgcccagct
    25741 cccggttcag ccgcaccagc aggttgaggt tgaactgtgc ggtcacgccg gcggcgtcgt
    25801 tgtaggcgcg ctccaggatg gcggggtgct tgaccaggtc cacgccgatc agcatcgcac
    25861 cgcccgggcc gacctcctgg cacgttgcac gcagcaggtc ggccgccatg gcgcgtggga
    25921 agttgccgat ggtcgagccg gggaaaaagg cgacgttgcg ccgcacccgg gagcgcttgg
    25981 gctcgggcag gtcgaagggc tcggtgaaat ccgcggtgac gggcagcacc tcgagctccg
    26041 ggaaatccgc ggcgatgcgt gccgctgcca cctgcaggtg ctcgcgcgag atctccaccg
    26101 gcacataggc ggnnnnnnnn nncgcctcga gccgggccag cagcagctcg gtcttgaccg
    26161 ccgagccgga gcccagctcg atcaggcgca cgccggggcc cagcgccgtg gccatttcgc
    26221 cgccatgggc ctcgaggatc ccgatctcgg tgcgcgtcgg atagtactcc ggcaatgcgg
    26281 tgatggcgtc gaacagctcc gagccacgct ggtcgtagaa gtacagcgag gggatgttcc
    26341 tggccggcag tgcgaggccg tgcagcacgt ccctgagcat gacctcgtcg tcggggctga
    26401 agtccgccac gccgcggagg ttctccatta cacctcgtcc ctggccaggc gcaggccgct
    26461 gaactgccag cgctgccccg ggtagaagaa gttgcggtag ctcgggcgca cgtggccgac
    26521 cggcgtcgca caggagccgc cacgcaacac catctggctg cacatgaact tgccgttgta
    26581 ctcgccgagc gagcccgcca gcggccggaa tcccgggtag ggtgcgtagg gactggcggt
    26641 ccactcccag gcgtcgccga acagctgctg gagcccctcg gcatgcgccg ccggccgcgg
    26701 gtgaaaggcg ccggtgccgg cgaaattgcc gtccaggggc cggccggtcg cagccgcctc
    26761 ccactcgaac tccgtcggca gccgggcccc cgcccagcgg gcgaaggcgt cggcctcgta
    26821 gtagctcagg tgcgtcaccg gggcgtccgg ctggaccggc tcgaggccgg caagggtgaa
    26881 ttcctgcgcc aggtcgtcct gccagtacag cggacgccgc cagccctcgg ccacgaccga
    26941 cgaccagccg tcggacagcc acagcgccgg ttctgaatag ccgccgtcgt cgatgaactc
    27001 gcgatactcg gcgttggtga cgagccggga ggccagcgaa tgctcgccca gcagcacccg
    27061 gtggcgcggc gtctcgttgt cgaaggcaaa atcctcgccg gcatggccga tctcgaccgg
    27121 gcccgcgcgc ccgcggatcc agcgcaaggg ggcggcgacc tcggcgaccg gcgcgggccg
    27181 ctcgacgtag gccggcagca acgggttctg ggcgagcagg tgcttgatgt cggtgagcat
    27241 cagttcctgg tgctgctgct cgtggtgcat gccgagcgtg accaggaact ccagctccgg
    27301 gttgccgcgc tcgcgctcca gcagcctggc gacggcggcg tccacgtgag cgcggtagtc
    27361 cagcacctcg gccagcgagg ggcgggtgat caggccgcgg cgcggccgca agtgcatctg
    27421 gccgacggtg aaatagtagg aattgaacag gtactcgaag cgctc
//
LOCUS       DAOMPG010000005        24399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_889_length_24399_cov_4.415626, whole genome shotgun sequence.
ACCESSION   DAOMPG010000005 DAOMPG010000000
VERSION     DAOMPG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 24399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24399
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_24399_cov_4.415626"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLS34_00595"
     CDS             complement(<1..306)
                     /locus_tag="PLS34_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRP86001.1"
                     /translation="MPRAAVIQMCSGPDVEANLANAGELLEAAAEAGAELAVLPENFS
                     FLGRGEADRVGVAERPGEGPAQGFLSARARELGLWIVGGTLPVLEPGLPARHAVERLP
                     "
     gene            complement(306..4295)
                     /locus_tag="PLS34_00600"
     CDS             complement(306..4295)
                     /locus_tag="PLS34_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HRP86002.1"
                     /translation="MAGLIRRLWRIAAGLLATLVVLLAVAVGLVRLALVQVPEYQAQI
                     ESWASDALGWPVEISSMDARLGWGGPQLRFTDARVLTRDGRLTLVHAAAGSMQIDTWS
                     LLRGRPRPGAVSFSGVALRVERNVDGRWGLLGADGPLLPERAPGADTVMPQLRDLPAG
                     DLVLEDVTLEFEDLRHGRGPWSFRIDDLELHLAAGELAISASGALPGELGGQMALSAV
                     LSKQDERGHPSDWTAGVSFSALNLPAVGAALGRPERLPVRGVLDGSLSLAAISGQLER
                     VAGEMLARDLLPRAGASQDELAVDDEPAIRGVEMPFERLAAKLEWQRKAGGWEVDLGE
                     LDVERGGRQWRSRVGVTYDVEASVRRVEARADHLELDDLALAAPWLPETARLMVDELA
                     PVGSVRDLEMHLDLPEDEARRPEIYLAATFQDMSIAPRGRWPGVRNLSGRIAGDLFGG
                     SATLDAPGSGIDLPWMFRAPLAFTAMQAAVEWSRNESGLEVRLAGLRLDNDDAVVDAQ
                     GRLYVPSGDVSPVLEMEAVARQVRLEAGPRYLPVNVLNSRVVAWLDAALVSGRVDEAR
                     FVFRGATREFPFREGDGLFKVEFELADGVLDFDPDWPDATGIQAGVRFENEGLWAEVR
                     EGRLLEVAAGPASVSIPDFRDKLLLIEGKAAGRLGAFREFALDAAMLKRILGPGLEPA
                     SMPTGEVVADVRLDLPLDAIADSRARVDLQISAGTVAFGFLGEPLRDVDARINIDNAR
                     VTGRDITATLAGSPLVADVVMTGGGAVRVEGGGRIDADGLARVLRLPLDTWVTGAADW
                     RGHLQFPGPDDATPLGFEFSSALVGFDIDLPEPFRKSAPESRRLVARASYPEGLLMDG
                     ELEWDDGLRIAARIDQSGESPVLRPVPGAVEGEPAGLVFSGAVKRLDLAEWLRVDWPT
                     GIDAKGVPGVIAGGRVLVGELSAPALQLHDVLLELARAGAGWRLGMAAERAAGDIEIP
                     FELYGDDPVTVRMARLWLGSGEAPAPGQEERPDAEPEPAVVIAPALVPPLDIEVDDLR
                     LGGVRLGRMSARILHEGDGFELIGLESVGDGFIIQAEGRSRLSDTVDASHLGLQIQSE
                     DVGATLEYMGFRRSMESRSGRFESRMEWQGGLRSDWFRAFTGNASIVIREGRLVGVEP
                     GAGRVFGLLSIQALPRRLALDFKDVFGEGTSFDRIAGDFDFVAGSAYTDNLVMEGPSA
                     NMIVVGRTGLVARDYDQTVVIGVDLGQTLPVAGAVVGGPAVGAALYLLSEIFRKPFQA
                     QITYRLTGTVENPVIERVSAGSLSPPQPPPPAVRPESRLEPQPQAPQPSPPPVQPPAD
                     RPENSPGGE"
     gene            complement(4332..5801)
                     /gene="rng"
                     /locus_tag="PLS34_00605"
     CDS             complement(4332..5801)
                     /gene="rng"
                     /locus_tag="PLS34_00605"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HRP86003.1"
                     /translation="MSEEILVNVNPREIRAALLENGVLQEVFIERASRRGLTSNIYKG
                     RVQRVLPGMQAAFVDVGLERTAFLHASDIISRLREPGIEADVEREPQIRELVEEGQDL
                     LVQVVKDPLGTKGARLTTFVTMPSRYLVHLPFGRGIGVSARIDDETERARLREILEDI
                     SSGRAGGWIVRTAAEHATRDALRADMLFLEKLWEAVDVEGRRSPAGTLVHSDLDLPLR
                     LLRDLLAPRVERVRVDDADEFARMKRFAASFIPEMEPKIQAYTEPRPIFDLYGVDDEI
                     ARALERKVPLKSGGYLVLDQTEAMTTIDVNTGAYVGHRNFEETIFRTNLEAAQAIARQ
                     VRLRNLGGIIIVDFIDMEEEDHRAQVLSALERALQNDHARNQITEVSRLGLVEMTRKR
                     TRESLEHVLCQPCPTCHGRGSVKTPETVCYEIFRDVLRQARQFEFQELLVLAHAEVLD
                     LLVDEEAAGLAELEALTGKPIRLQAESGYSHDQYDVVPI"
     gene            complement(5798..6400)
                     /locus_tag="PLS34_00610"
     CDS             complement(5798..6400)
                     /locus_tag="PLS34_00610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRP86004.1"
                     /translation="MDSVQLVLASGSPRRLELLASLGLECEVSAVDIDEAPLAGESPA
                     GCARRLAAEKAAAAVRRHGPGVLVLAADTVVALGEEMFGKPADAADAARMLRRLSGRT
                     HQVHTAVAASRSGKTSFRLSTSEVTFRDLRAAEIDAYVATAEPLDKAGGYGIQGLAAV
                     FVTRLCGSHSGVVGLPLSETAQLLHQHGIDVLQLAAGRLA"
     gene            complement(6450..6917)
                     /gene="rlmH"
                     /locus_tag="PLS34_00615"
     CDS             complement(6450..6917)
                     /gene="rlmH"
                     /locus_tag="PLS34_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRP86005.1"
                     /translation="MRLRLVAVGTRMPGWVDEGFREFAGRMPRECRLELREIALGRRS
                     RGADTGRAKAEEADRLLAASTGCFRVCLDVRGEQLDTPGLARRLAAWQQGGQDVALLV
                     GGPDGLAAPCLEAAQWRWSLSPLTLPHGLVRVLLAEQLYRAWTVLTGHPYHRE"
     gene            complement(6924..7277)
                     /gene="rsfS"
                     /locus_tag="PLS34_00620"
     CDS             complement(6924..7277)
                     /gene="rsfS"
                     /locus_tag="PLS34_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRP86006.1"
                     /translation="MSDEALQKTVLDALDDLKGRDVVVLDVRGLTSIADSMVIASGTS
                     DRHVRSLAENVIEKCKEGGHRPLGVEGLKDGEWVLVDLQDIVVHVMLPRVRDFYNLEK
                     LWLHPTKTGRASAEA"
     gene            complement(7277..7945)
                     /gene="nadD"
                     /locus_tag="PLS34_00625"
     CDS             complement(7277..7945)
                     /gene="nadD"
                     /locus_tag="PLS34_00625"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRP86007.1"
                     /translation="MSSLEDPGPIGLFGGTFDPIHYGHLRTAFELLEAIEFAELRFLP
                     AGNPPHRDRTHADAVTRLALVRAAIEGEPRFMLDDRELRREGPSYSVDTLLDLRAEHA
                     HRSLCLVVGMDAFLGLPQWHQWREILQLAHLVVAHRPGWQAPDTGTLGELLAERGTRR
                     ATDLHEARAGRVYVRAVTQLEISSSAIRELVRAGREPRYLVPDTVCRLLAGSGCYSAA
                     AEGN"
     gene            complement(7942..9009)
                     /gene="holA"
                     /locus_tag="PLS34_00630"
     CDS             complement(7942..9009)
                     /gene="holA"
                     /locus_tag="PLS34_00630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRP86008.1"
                     /translation="MTLSPAQLEAGLARRLAPAYLIAGDEPLLIQECADAVRRKAREQ
                     GVAEREVHAVERGFDWAAFGGGLASLSLFASRRLVELRLRTPASAADGAEVFARFAEQ
                     PPDDVVLLVIAPKLDKRAQGAAWVKALSRAGEFIEVRPIEPPALPSWIQERLRRAGLR
                     ADAEAVRLLAERVEGNLLAAHQEIEKLRLLHGEGEVDAEGVRAAVADSARYDIFKLAD
                     AAVAGDLGRALRILAGLRAEGLAIPQVLWPLARELRGLARVRWLMDEGLAPQAAMQRA
                     GIWRNRTGLVGRALARHDGRALRALARRLALVDRLSKGALPGNAVPAGRNEGAVRAGC
                     DPWAELVDFVAELAGPVRPSA"
     gene            9141..9446
                     /locus_tag="PLS34_00635"
     CDS             9141..9446
                     /locus_tag="PLS34_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86009.1"
                     /translation="MKQQTAILPTCLQRFAWMVALVVVLRVVAWDLHHAFDLHGESDP
                     RCQVCLVMERGGDGVAPVTVVAVACPCGPAPLPMVSALVPVAPALGPLPRGPPSSFS"
     gene            9529..10917
                     /locus_tag="PLS34_00640"
     CDS             9529..10917
                     /locus_tag="PLS34_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP86010.1"
                     /translation="MKNMLLAGAIAALCAAPSLAETTGPQRDPLDPKNLRQTTLPLGS
                     QVSSGTAFNPAMSVIIDGAYYSDNVHGEAFEILEEADGFAMGHGHDHEGEEHGHGGLE
                     RGFNLREVEFAFSGTVDPYFDAYVMFVFDGDEIELEEAFLTTRSLPAGLQAKFGKFLS
                     DVGYINKQHPHSWDFFDAPLVSELLFGDHGIQEVGVQLTWLAPTDQYLRLGIEALQGE
                     TAGIANYFGAQGDIEGTERILEDAAGPRLFTGFAKFSPNLGFDHALQLGASYGYSSSF
                     QNSVEHSTRYEDHEGTASFWGVDAVYKYTPPGSVGCKGAVKLQGEYFYRKRDIDRQDV
                     YFACHPAGIDCADPAAQWQIGDINNVESFSEKQDGYYLQALYGLAPRWNVGARIDQVG
                     LTNSTGRGAGEEWDDSKRYSAVLNFMPTEYSRLRLQFSRGDIAVEGERETFNQVFLQF
                     QMSLGVHGAHTF"
     gene            10928..11809
                     /locus_tag="PLS34_00645"
     CDS             10928..11809
                     /locus_tag="PLS34_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRP86011.1"
                     /translation="MRKLLLTLVLCLGAPLPAAALQVTATTPNMGMLARTVGGQHVKV
                     VLLAPGDRDVHHLEARPSMMVALRRADLLVAVGGELEVGWLPAALRGAGNPAILPGRP
                     GYFEAAAAVTLLGAGAVASRAGGDVHPMGNPHIYFDPLRMAAAAEALAEQLAVLDEAN
                     AAAYAANARAFSARMQQEVAGWRVRVASSPGAVPYHKDADYLFAALEVPVLGYLEPLP
                     GIPPTARHIRELVDELQGKQGVIFHMVYEPAAGPERLARELGWPVFRMRNNVPEQGTA
                     DDYVALIEQWVARLETR"
     gene            11806..12378
                     /locus_tag="PLS34_00650"
     CDS             11806..12378
                     /locus_tag="PLS34_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP86012.1"
                     /translation="MNTAVPLLELIDVRAGYSRPVVGPVSFVVRPGEVLGLQGANGIG
                     KTTVIKAITGGARLFGGELRRTAGLTITHHWQRPERPPELPLTGLEMLRVTSAATASL
                     PPRLHALCARCLDEMSGGEFQLLHAWACLTAPVRLVLLDEPTNNLDQDAIELLLTEIG
                     KRPPGRAVLLVSHETDFLRRACDRIVPVCP"
     gene            12369..13172
                     /locus_tag="PLS34_00655"
     CDS             12369..13172
                     /locus_tag="PLS34_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRP86013.1"
                     /translation="MSLNLAWDPLFRLPLATALVLAATLPLIGAGLRLREQWFSSLGV
                     AQMAAAGGVAGALLHGPIMLFALLGAALGGAARALAGRTRNEHYAVLLLAGWAAVILL
                     ALQGHHADTTALQLLNGQLYFTALPHLLGAIALLGALLACGRWLARRLLLARLFPDHF
                     AANGRPTWPHELCFEGLLIAAVVLGISTMGVMATFAMMFLPPWVAFRLARGWPRALLL
                     SALIGLVACLAGFMLALALDLPFGAALVAVLLLSVPLRLAPVRFGRQAD"
     gene            complement(13122..14261)
                     /locus_tag="PLS34_00660"
     CDS             complement(13122..14261)
                     /locus_tag="PLS34_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HRP86014.1"
                     /translation="MSVAALMLVAGTVIDPAALARFADMALSCVHREYPNKIAHVLAS
                     DADVAPPRELTPVFYGCFDWHSSVHGHWLLARLARFEPDAPFTPAARAALERSLTPEG
                     IRAEVEYLAAPGREGFERPYGLAWLLQLGAELREWNDPDAARWAVALAPLEALASSRL
                     REWLPKLAYPIRGGEHFQTAFAFGLALDWARTAGDAELAELLIGTSRRLYLADRDCPL
                     AYEPSGHDFLSPCLAEADLLRRVLPPAEFADWLAAFLPDIPEDTDDGWLPVAQVTDRA
                     DGKLAHIDGLNLSRAWMLEGIAAALPPGDLRRITLRATAARHARAGLEGVSSEHYAGS
                     HWLGSFATYLATGRGLPPAARDCACLADDSVGLAPESDGGQPQGD"
     gene            complement(14258..14611)
                     /locus_tag="PLS34_00665"
     CDS             complement(14258..14611)
                     /locus_tag="PLS34_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004736268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRP86015.1"
                     /translation="MPMPTLFEKIIAGELPADFLYRDERVVAFRDINPQAPVHVLIIP
                     VKPIPTANDIDDEDAGLIGHMFCVARDLARAEGIADDGYRLIINCNSHGGQEVYHLHL
                     HLLGGRRLGRMLPPQ"
     gene            14735..15196
                     /locus_tag="PLS34_00670"
     CDS             14735..15196
                     /locus_tag="PLS34_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRP86016.1"
                     /translation="MATRLTRFAVAVVVLAAAGCANLAALQAPDVQVTSLQLLEPAPG
                     SLEQRFAVGLRLINPNNRALAVDGLDFELELNDRRLARGVTNRAFELPRLGEAETSVI
                     VTTSVFDVLRQAVELGSRRESAMDYRLRGRLHLGSGFVRTVPFDHRGKLTP"
     gene            complement(15210..15710)
                     /locus_tag="PLS34_00675"
     CDS             complement(15210..15710)
                     /locus_tag="PLS34_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86017.1"
                     /translation="MNASEVLTLADGHEDAVREVLARFGLALQIVPAGESIPGSWWGE
                     REAGLRDAVLYARADTPLHSVLHEACHFVCMSPERRAELDTNAGGDYDEENGVCYLQA
                     LLADELPGFGRLRMFADMDAWGYSFRLGSARAWFEGDAEDARHWLRAHGLIDLHDRPV
                     WRLRAA"
     gene            complement(15707..16198)
                     /locus_tag="PLS34_00680"
     CDS             complement(15707..16198)
                     /locus_tag="PLS34_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86018.1"
                     /translation="MRRPGTFLLVLISLALAACGYHLQGVTRYPPELAAVHLDVPDPT
                     SDLAVQLRRSLTASGVTLARDATDATATLSIVTERFGRTVKSVSAQNRPTEYEVFYQA
                     EYTLATLAGTVVAPQSIARTRIFPYSERDILGKQQEEELLRESLAREIAGVITRRLAG
                     LSD"
     gene            complement(16202..18799)
                     /gene="leuS"
                     /locus_tag="PLS34_00685"
     CDS             complement(16202..18799)
                     /gene="leuS"
                     /locus_tag="PLS34_00685"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019612585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRP86019.1"
                     /translation="MDPIYKPEVIEPAAQRYWDEQGCFEAGEDPSKEKFYCLCMFPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMQGKNVLQPMGWDAFGLPAENAAIKHGVPPARWTR
                     ENIAHMRAQLRSLGFAYDWRRELATCDPDYYRWEQWFFLRLYEQGLVYKSSSTVNWDP
                     VDQTVLANEQVIDGRGWRSGALVERREIPQWFLKITAYADELLEALDTLPGWPEQVRT
                     MQANWIGRSEGLELDFALDGAPVAGYRALTVYTTRPDTLFGVTYMAVAAEHPLAVHAA
                     QGNTELAAFLAECRSAHTTEAALETMEKRGMPLEVSVVHPLTGDRVPVWVANFVLMGY
                     GTGAVMAVPGHDRRDWEFATKYGLPIRQVIAPADGSAADLSDGAYVEYGVLVNSPGFE
                     GMSSAEAFEALAMKLEGEGRGRRRVNYRLRDWSVSRQRYWGCPVPMIDCPSCGAVPVP
                     EDQLPVRLPEDVTLSGAGSPLKQMDEFVRVDCPQCGGPARRETDTFDTFFESSWYYAR
                     FCSADQDAAMLDDRARYWLPVDQYIGGIEHAILHLLYARFFHKLMRDEGLVDGDEPFA
                     NLLTQGMVLADSFYRDDAKSGRSWYNPAEVEVQRDDKGRVLSATLTADGAPVELGGMG
                     TMSKSKNNGVDPQALIDRYGADTVRLFTMFAAPPEQSLEWSDSGVEGAQRFMKRLWRM
                     VHEHVGEGPAPALEAGALDEAQRELRRQLHATIAKVSDDVGRRYTFNTAIAAVMELLN
                     SLGRHAGRELQDRALRQEAFEAVTLMLSPIVPHACHALWHALGRAGAVVDQPWPAHDP
                     AALVREEVELVVQVKGKLRGRIRIARDASEAAIRSAALAEENVARFVADSPVRKVIIV
                     PGRLVNIVV"
     gene            complement(18831..19994)
                     /gene="lnt"
                     /locus_tag="PLS34_00690"
     CDS             complement(18831..19994)
                     /gene="lnt"
                     /locus_tag="PLS34_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRP86020.1"
                     /translation="MTPCSDGWARAGCRPAGWLAVEWLRGWFLSGFPWLSLGYSQTDS
                     VLGALAPLGGVYGLSWVCALGAGALAAVLVEGLAGRALAVATLFVVFAAAGHAGGKSW
                     TAPVGEPLRAVMVQGAIGQDEKWLEASLEPTRQLYLDMTRPHWGADLVIWPEAAIPAL
                     LHLEMDFIEALDEEARAAGSEILLGILERDRQSGQYYNVLVSLGGQPEIYRKRQLVPF
                     GEYFPVPAFVRRWMRLMNLPYTDFAPGRADPPPMTLAGVKVAPTICYEDAFGAQQRVF
                     FPAAELLVNVSNDAWFGDTRAPHQHLQIARMRAMETGRWMLRATNNGVTAVIDPRGRV
                     TQRSRQFVAEVIEAEVPPMTGLTPWLRYGDGPLAAVALGLFLLGTLRRNTAGR"
     gene            complement(20321..21169)
                     /locus_tag="PLS34_00695"
     CDS             complement(20321..21169)
                     /locus_tag="PLS34_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRP86021.1"
                     /translation="MTDDKPPSTRGRRSGLLARWAGAFRAAPRDRQALIEMLDQARRS
                     RILDADEFSMLEGVLEVATIQVRDIMIPRGQMVVVERNASPQEMIQIIIDSGHSRFPV
                     IADDRDEVIGILLAKDLLRFYAENERERFHIKECLRPAVFIPESKRLNVLLKEFRVSH
                     NHMAIVVDEYGGVSGLLTIEDVLEQIVGEIDDEHDPDEEVMIVPEQQDAWRVDALTRI
                     EDLNEELGTNFSDADYDTVGGLVMTELGRLPRKGESLVLDGMRFTVLRADRRRVHAVR
                     LRRAAG"
     gene            complement(21162..21632)
                     /gene="ybeY"
                     /locus_tag="PLS34_00700"
     CDS             complement(21162..21632)
                     /gene="ybeY"
                     /locus_tag="PLS34_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005329828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRP86022.1"
                     /translation="MSLRIDVQVGGTRTGLPGVAKLRHWARSALAGRRDDAELSIRIV
                     DAAESRALNRRYRGKDRPTNVLAFPAELPAELGLPLLGDLVICREVVEAEATEQAKPL
                     DAHWAHMVIHGTLHLLGFDHQTDEQAATMEALEADLLAELGWPNPYREREPNDD"
     gene            complement(21629..22591)
                     /locus_tag="PLS34_00705"
     CDS             complement(21629..22591)
                     /locus_tag="PLS34_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRP86023.1"
                     /translation="MNANLVRDLVLEPADNQRLANLCGQFDEHLKQIERRLGVSIGSR
                     GNCFQIEGPERAVDATERLLVALFEQAATESINPQRVHAWLQEAGLYEEPGGGAPGEL
                     RMRRGVIRGRGPNQARYIQSMRDNDLCFGIGPAGTGKTYLAVASAVEALERDAVRRIV
                     LVRPAVEAGERLGFLPGDMSQKVDPYLRPMYDALYEMLGFERVGKLIERHVIEIAPLA
                     FMRGRTLNEAFVILDEAQNSTVEQMKMFLTRIGFGSRAVVTGDVTQTDLPASKLSGLK
                     HASDVLKGVEGVAFTWFTARDVVRHPLVQRIVEAYQRHQGDEQQ"
     gene            complement(22606..23973)
                     /gene="miaB"
                     /locus_tag="PLS34_00710"
     CDS             complement(22606..23973)
                     /gene="miaB"
                     /locus_tag="PLS34_00710"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRP86024.1"
                     /translation="MSQDKPGGKLFIKTFGCQMNEYDSSKMGDVLRASHGLEAAASPE
                     EADILLLNTCSIREKAQEKVFSLLGRWRELKEARPGVVIGVGGCVASQEGEAILGRAP
                     FVDIVFGPQTLHRLPAMLEEVRGRGRAAVDISFPEIEKFDRLPAPRAEGPTAFVSVME
                     GCSKYCSFCVVPYTRGEEVSRPFDDVIVEVAQLAAQGVREINLLGQNVNAYRGVMHDG
                     THADLAALIRFVAAVDGVDRIRFTTSHPVEFTDSLVAAYAEVPELAPFLHLPVQSGSD
                     RVLALMKRGHTALEYRQKIRRVREARPGISISSDFIVGFPGETERDFEQTLQLIDDIG
                     FDQSFSFIYSRRPGTPAAALPDDVDMAEKQRRLALLQHRINQRAAQISRAMVGGVERV
                     LVERPSKKNPRQLAGRTGNMRWVNFDAEPALVGQFADVLITEALPNSLRGRVVGVDGA
                     LARSA"
     gene            complement(23984..>24399)
                     /gene="ssb"
                     /locus_tag="PLS34_00715"
     CDS             complement(23984..>24399)
                     /gene="ssb"
                     /locus_tag="PLS34_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRP86025.1"
                     /translation="LGADPESRTTPGGLTVTNIRVATTEKRKDRQSGDLVEETEWHRV
                     VMFDRLAEVAREYLRKGSQVYIEGQIRTNKWQDKDGNDRYTTEIIARDMQMLGGRPGA
                     GAPAGGDDDYRQQPSGGRSSGGARKEEPFEDDIPF"
BASE COUNT         3772 a         8746 c         8223 g         3658 t
ORIGIN      
        1 aggcagacgt tctacggcat gtcgcgctgg caggccgggc tcgagcaccg gcagcgtgcc
       61 gccgacgatc cacaggccga gctcgcgcgc ccgcgccgac aggaagccct gtgcggggcc
      121 ctcgccgggc cgctccgcca cgccgacccg gtcggcctcg ccacggccga gaaacgagaa
      181 gttctccggc agcacggcca gctcagcgcc tgcctcggcc gcggcctcga gcaattcacc
      241 ggcatttgcg aggttggcct cgacgtcggg cccggaacac atctggatca cggcggcacg
      301 cggcatcact ctcctcctgg cgaattctcg ggccgatcgg cgggcggttg caccggcgga
      361 ggcgacggct gcggcgcctg gggctgcggc tccagccgcg attccggtcg caccgcgggc
      421 ggcggcggct ggggcgggct caacgagccg gccgagaccc gctcgatgac cgggttctcg
      481 acggtgcccg taaggcgata ggtgatctgc gcctggaacg gcttgcggaa tatttccgac
      541 aacaggtaca gcgccgcgcc caccgccggg ccgccgacga ccgcgcccgc cacgggcagg
      601 gtctggccga gatccacgcc gatgaccacc gtctggtcgt agtcgcgggc cacgaggccg
      661 gtgcgcccca ctacgatcat gttcgccgac ggcccctcca tgacgaggtt gtcggtatag
      721 gcgctgcccg cgacgaaatc gaagtccccg gcgatgcgat cgaacgacgt gccctcgccg
      781 aaaacatcct tgaagtccag cgccagccgc cgcggcagcg cctggatact gagcaggccg
      841 aacacgcggc cggcgccggg ctcgacaccg accagccgac cctcgcggat gacgatactg
      901 gcgttgcccg tgaaggcgcg aaaccagtcg ctgcgcaggc caccctgcca ctccatgcgt
      961 gactcgaagc ggccgctgcg cgactccatg ctgcggcgga accccatgta ctccagggtg
     1021 gcgcctacgt cctcgctctg gatctgcagc ccgaggtgtg acgcgtcgac ggtatcggac
     1081 aggcgcgaac gtccctcggc ctggatgatg aagccgtcgc cgacgctttc caggccgatc
     1141 agttcgaagc cgtcaccttc gtgcagtatg cgggccgaca tccgaccgag ccgcacgccg
     1201 ccgagtcgca ggtcatcgac ctcgatatcg agaggcggca ccagcgccgg tgcgatgacc
     1261 acggcaggct ccggctccgc gtccgggcgc tcctcctggc cgggagccgg cgcctcgccc
     1321 gacccgagcc agagacgcgc catgcgcacc gtgaccggat catcgccata cagctcgaag
     1381 ggaatctcga tatcgcctgc cgcccgctcg gctgccatgc cgaggcgcca gccggcgccc
     1441 gcgcgcgcca gctccagcag cacgtcgtgc agctgcagcg ccggggccga cagctcaccc
     1501 accagcaccc gaccgccggc gatgacgccc ggcacgccct tcgcgtcgat gccagtcggc
     1561 cagtcgaccc tgagccattc ggccaggtcg agccgcttga cggcgccgct gaaaaccagg
     1621 cccgcgggct cgccctcgac ggcgcccggc accggcctga gcaccggcga ctcgcccgac
     1681 tgatcgatgc gggccgcgat gcgcagccca tcgtcccact cgagctcgcc gtccatgagc
     1741 aggccctccg gataggaggc cctggcgacc agtcggcggc tttccggggc ggacttccga
     1801 aatggctccg gcagatcgat gtcgaatccg accagcgcgg aagaaaattc gaatcccagc
     1861 ggcgtagcgt cgtcgggacc gggaaattgc aggtggccgc gccagtccgc ggcaccggtc
     1921 acccacgtgt ccagcggcag gcgcagcacc cgggccaggc catcggcatc gatccgccct
     1981 ccgccttcga cccgcacggc accgcccccg gtcatcacga cgtcggccac cagcggcgac
     2041 ccggccaggg tggcggtgat gtcgcgaccc gtgacccgcg cgttgtcgat gttgatgcgc
     2101 gcgtccacgt cgcggagggg ttcgccgaga aagccgaatg cgaccgtccc cgcgctgatc
     2161 tgcaaatcga cccgggcgcg ggagtcggcg atggcgtcga ggggcaggtc cagccggacg
     2221 tctgcgacta cctcgccggt cggcatgctc gcgggctcca gaccggggcc gaggatgcgc
     2281 ttcagcatcg ctgcgtcgag ggcgaattcc cggaaagcgc cgagccggcc cgcggccttg
     2341 ccctcgatga gcagcaactt gtcgcggaag tctggaatcg acacgctcgc gggacccgcc
     2401 gccacctcga gcaggcgccc ctcccgcacc tccgcccaca atccctcgtt ctcgaagcgc
     2461 accccggcct ggatgccggt ggcgtccggc cagtccggat cgaagtccag cacgccgtcc
     2521 gccagctcga actcgacctt gaacagcccg tcgccctcgc gaaacgggaa ctcgcgagtc
     2581 gccccccgga acacgaaacg cgcctcgtcg acccggccgc tgaccagcgc cgcatcgagc
     2641 cacgcgacga cccggctgtt cagcacgttg accggcaagt agcgcggacc ggcctccagc
     2701 cggacctggc gcgccaccgc ctccatctcg agcaccggcg aaacatcccc ggaaggcaca
     2761 taaagccgtc cctgggcatc caccacggcg tcgtcattat ccagtcgaag tcccgcgagg
     2821 cggacttcca gcccgctctc gtttcgcgac cactcgacgg ccgcctgcat tgcggtgaag
     2881 gccagcggtg cgcgaaacat ccatggcagg tcgattcccg acccaggggc atccagcgtg
     2941 gcgctgccgc cgaacaggtc gccggcaatt cggccggaca ggttccggac gccgggccag
     3001 cgcccccgcg gcgcaatcga catgtcctgg aaagtggcgg cgagatagat ctccggtcgt
     3061 cgcgcttcat cttccggcag gtcgagatgc atttccaggt cgcgcacgct gcccacggga
     3121 gccaactcat cgaccatcag gcgagccgtc tccggaagcc agggtgcggc aagcgcaagg
     3181 tcgtcgagtt cgaggtgatc cgcccgggct tcgacgcggc gcacggacgc ctcgacgtcg
     3241 taggtcacac cgaccctgct gcgccactgc cggccgccgc gctcgacatc cagctccccg
     3301 aggtcgacct cccagccgcc tgccttgcgc tgccattcca gcttcgcggc gagccgctcg
     3361 aagggcattt ccacaccgcg gatcgccggc tcgtcgtcaa cggcgagttc atcctgtgac
     3421 gcgccggcgc ggggtaacag gtcgcgcgcg agcatctcgc cggccacgcg ctcgagttgg
     3481 ccagagatgg ccgccaacga caggctgcca tccagtactc cgcgcaccgg cagtcgctcc
     3541 gggcggccga gcgccgcacc gaccgccggc aggttgagcg ccgagaagga cacacccgcg
     3601 gtccagtcgc tgggatggcc gcgctcgtcc tgtttcgaca acacggccga cagcgccatc
     3661 tggccgccga gttcgccggg cagcgcgccc gaggccgaga tggcgagctc acccgcggcc
     3721 agatgcagct ccaggtcgtc gatccggaac gaccacgggc cgcggccatg ccgcaggtcc
     3781 tcgaactcca gcgtcacgtc ctccagcacc aggtcgccgg ccggcaggtc gcgcagctgc
     3841 ggcatgacgg tgtcggcgcc cggcgcgcgc tccggcaaca gggggccgtc ggcaccgagc
     3901 agcccccagc ggccatcgac gttgcgctcg actcgcaatg ccacgcccga gaagctgact
     3961 gcgcccggcc gcggccggcc acgcaacagc gaccaggtat cgatctgcat cgatcccgcc
     4021 gcggcgtgga ccagggtgag ccgaccgtcc cgggtcagta cgcgcgcatc cgtgaagcgc
     4081 aactgtggcc cgccccaccc gaggcgggca tccatggagc tgatctcgac cggccagccg
     4141 agcgcgtcac tcgcccagga ctcgatctgc gcctggtact ccggcacctg caccagcgcg
     4201 agccgcacca ggcccacagc cacggccagc aggaccacca aggtggccag caagccggcg
     4261 gcaatccgcc acaacctgcg aatcagtccg gccaccttgc ccccggatgg cccggacgat
     4321 cgatcgtcac cctagatcgg aaccacatcg tactggtcgt gcgaatagcc ggactcggcc
     4381 tgcaggcgga tcggcttgcc ggtcagggcc tcgagctcgg caaggccggc tgcctcttcg
     4441 tcgaccagga ggtccagcac ctcggcgtgg gccagcacca gcagctcctg gaactcgaac
     4501 tgccgcgcct ggcgcagcac gtcgcggaaa atttcatagc agaccgtctc gggcgtcttg
     4561 acggagccgc gcccgtggca ggtggggcac ggctggcaca gcacgtgctc gaggctttcc
     4621 cgcgtccgct tgcgcgtcat ctccacgagg cccagccggg acacctccgt gatctggtta
     4681 cgggcatgat cattctgcag ggcgcgctcc agggccgaca gcacctgcgc gcggtgatcc
     4741 tcctcttcca tgtcaatgaa atcgacaatg atgatgccgc cgaggttcct gagccgtacc
     4801 tgcctggcga tcgcctgggc agcctcgagg ttggtcctga aaatggtctc ctcgaaattg
     4861 cggtggccca catacgcgcc ggtgttgacg tcgatggtcg tcatcgcctc ggtctggtcc
     4921 agcaccaggt agccgcccga cttgagtggc accttgcgct cgagggcgcg tgcgatctcg
     4981 tcgtccacgc cataaaggtc gaatatcggc cgtggctccg tatacgcctg gatcttgggt
     5041 tccatctccg gtatgaagct cgcggcgaag cgcttcatgc gggcaaattc gtcggcatcg
     5101 tcgacgcgca cccgctccac ccgcggcgca agcaggtcgc gcagcaatcg cagcggcagg
     5161 tccaggtcgg aatgcaccag cgttcccgcc ggactgcggc gaccctcgac gtcgacggcc
     5221 tcccacagct tttcgagaaa cagcatgtcg gcacgcagcg cgtcccgcgt cgcgtgctcg
     5281 gccgcggtgc gcacgatcca tccgccggca cggccggagg aaatgtcctc gaggatttca
     5341 cgcaggcggg cgcgctctgt ctcatcgtcg atccgcgccg acacgccgat gccgcgacca
     5401 aacggcaggt gcaccaggta acgcgacggc atggtcacga atgtcgtcag gcgggcgccc
     5461 ttggtgccca gcgggtcctt gacgacttgt acgagcaggt cctggccttc ctcgaccagt
     5521 tcgcgaatct gcggctcgcg ctcgacgtcc gcctcgatgc ccggctcgcg gaggcgcgag
     5581 atgatgtcgg aagcatgcaa gaaggcggtc cgttcgaggc cgacatccac gaaggcggcc
     5641 tgcatgcctg gcaatacgcg ctgcactcgg cccttgtaga tgttgctggt gaggccgcga
     5701 cgactggcgc gctcgatgaa gacttcctgg agcacgccgt tttccagcag cgcggcgcga
     5761 atctcgcgcg gattgacgtt gacgagaatc tcctcgctca tgccagcctc cccgcggcca
     5821 gctggagcac gtcgatgccg tgctggtgca gcagctgggc ggtctcgctg agcggcaggc
     5881 cgacgacgcc cgagtggctg ccgcagagcc gggtgacgaa taccgcggca agcccctgga
     5941 tcccgtagcc gcccgccttg tccagcggtt cggcggttgc gacgtacgcg tcgatctccg
     6001 ccgccctgag gtcgcggaag gtcacctcgc tggtcgagag ccggaacgaa gtcttcccgc
     6061 tgcggctggc ggcgactgcg gtatgcacct ggtgggtacg tccggagagg cgtcgcagca
     6121 tgcgcgcggc gtccgcagcg tcggccggct tgccgaacat ctcctcgccc agggccacca
     6181 ccgtatcggc ggcgaggacc agcacgccgg ggccgtggcg ccggacggcc gcggcggcct
     6241 tctccgccgc cagccggcgg gcacagccag ccggcgactc gccggccagc ggcgcctcat
     6301 cgatgtccac ggcggagacc tcgcattcga gcccgagcga agcgagcagc tccaggcgcc
     6361 gcggcgaacc cgaagccaac accagttgta cagaatccat gcggccagtt tccccgctga
     6421 gcgtcaccgg cacaagcggg cgcagcggct cactcgcggt gataaggatg acccgtaagc
     6481 accgtccagg cgcggtacag ctgctcggcg agcagcaccc gtaccaggcc gtgcggaagc
     6541 gtcaggggcg acagcgacca gcgccactgc gccgcctcca ggcagggcgc ggcgagcccg
     6601 tccgggccgc ccaccagcag ggccacgtcc tgcccgccct gctgccacgc agccagccgg
     6661 cgggccagtc cgggggtgtc gagctgctcc ccccgcacgt ccaggcaaac ccggaagcag
     6721 cccgtcgagg cggccagcag gcggtcggcc tcctcggcct tggcgcgccc cgtatcggcg
     6781 ccccggctgc gacggcccag tgcaatctcc ctgagttcga gccggcactc gcgcggcatg
     6841 cgtccggcaa attccctgaa accctcgtcc acccagccgg gcatgcgggt accgacggcc
     6901 accaggcgca gccgcaccgg ggatcaggcc tcggccgagg cgcgaccggt cttcgtcggg
     6961 tgcagccaga gtttctcgag gttatagaaa tcgcgcaccc gcggcagcat gacgtgcacc
     7021 acgatgtcct gcaggtccac cagcacccac tcgccgtcct tcagtccctc gacgcccagc
     7081 ggacgatgac cgccctcctt gcatttctct atgacgtttt cggccagcga ccggacatga
     7141 cgatcggagg tgccgctggc gatcaccatg ctgtcggcaa tgctggtcag gccgcgtacg
     7201 tccagcacga cgacgtctcg ccccttgaga tcgtcgagcg cgtccagaac cgttttctgc
     7261 aaggcttcgt cggacatcaa ttcccctctg cggctgcgct gtaacaaccc gatccggcga
     7321 gcagccggca gaccgtatcg ggtaccaggt aacgcggttc gcggcccgct cggaccagtt
     7381 cgcgaatcgc gctcgatgag atttccagtt gcgtcaccgc gcgcacgtac actcgcccgg
     7441 cgcgcgcctc gtgcaggtcg gtcgcgcgcc gggtgccacg ctccgccagc agttcgccga
     7501 gcgtgcccgt gtccggcgcc tgccacccgg ggcgatgcgc caccaccagg tgcgccagct
     7561 gcaggatctc gcgccactgg tgccattgcg gcagccccag gaaggcgtcc atgccgacaa
     7621 ccaggcacag cgagcgatgg gcgtgctccg cacgcaggtc gagcagcgtg tccacggaat
     7681 aggacggacc ctcgcggcgc agctcgcgat cgtcgagcat gaagcgcggc tcgccctcga
     7741 tggcggcccg caccagcgcc agccgcgtga cggcatccgc atgggtgcgg tcgcggtgcg
     7801 gcgggttgcc cgcgggcagg aaacgcagct ccgcgaactc gatcgcttcc agcagctcga
     7861 aggcggtgcg caggtggccg taatggatcg gatcgaaggt gccgccaaac aggccgatcg
     7921 ggcccgggtc ctcgaggctg ctcatgcgga cggtcgcacc ggtccggcca gttcggctac
     7981 gaaatcgacc agctccgccc acgggtcgca gccggcccgt acggcgcctt cgttgcgccc
     8041 cgccggcacg gcgttgcccg gcagcgcccc cttggaaagc cggtccacca gcgcaaggcg
     8101 ccgggccagg gcacgcagcg cccgaccatc gtggcgggcc agcgcgcgtc ccaccaggcc
     8161 ggtgcgatta cgccagatcc cggcgcgctg catggctgcc tgcggcgcga ggccctcgtc
     8221 catgagccag cgcacccggg ccagcccgcg caactcgcgc gccagcggcc agagaacctg
     8281 ggggatggcc agcccttcgg cgcggagccc cgccaggatg cgcagcgccc ggccgaggtc
     8341 gccggccacc gccgcgtcgg ccagcttgaa aatgtcgtag cgcgcgctgt ccgctacggc
     8401 cgcacgcacc ccttcggcgt ccacttcacc ttcgccatgc aacaggcgca gcttttcgat
     8461 ctcctggtgc gcggccagca ggttaccctc cacccgctcg gccagcaggc gcactgcctc
     8521 ggcatccgcg cgcaggccgg cgcggcgcag gcgttcctgg atccaggacg gcagggcggg
     8581 cggctcgatg ggtcgcactt cgatgaattc gccggcccgg gagagcgcct tgacccatgc
     8641 cgcgccctgc gcccgcttgt ccagcttcgg cgcgatcacc agcaagacga cgtcatcggg
     8701 aggttgctcg gcgaagcgcg caaacacctc ggcgccgtcg gccgcggacg ccggcgttcg
     8761 caggcgcagc tcgaccaggc ggcgcgaggc gaacagcgac aggctggcaa ggccgccgcc
     8821 gaaggccgcc cagtcgaagc cccgctcgac cgcgtgcacc tcgcgctccg cgacgccctg
     8881 ttcccgggcc ttgcgacgaa cggcatccgc gcactcctgg atgagcagcg gttcgtctcc
     8941 ggcgatcagg taggcgggcg cgaggcgacg ggcaaggccg gcctcaagct gtgcaggcga
     9001 gagggtcacg ggcgaaatat ctgttcccgg gaagtttcat cgcaggggca gtgtagcacg
     9061 ccgaaaccag gcgcaatccc cggattcccg ggccgggttg gacttgacgg ccgtgatatc
     9121 gtacaaaatt ggatcgcccc atgaagcaac aaaccgccat tctgccgacc tgcctgcagc
     9181 gcttcgcctg gatggtggcg ctggtggtcg tgctgcgcgt ggttgcctgg gacctgcacc
     9241 acgcgttcga cctgcacggc gagtcggacc cgcgctgcca ggtctgcctg gtcatggagc
     9301 gcggcggtga cggcgtcgcg ccggtcaccg tggtggccgt cgcgtgccct tgcgggcctg
     9361 ccccgctgcc gatggtctcc gcactcgtcc ctgttgcgcc tgcccttggc ccgctgccgc
     9421 gcgggccacc ctcgtcgttc agctgacgtc accgggagac cgctcccggc cgcagtgacc
     9481 ggcccccggg ccggtccgta cagctgaata ccatgcgagg aacacaccat gaagaatatg
     9541 ctcctggccg gcgccatcgc cgccctgtgc gcggcgccgt cactggctga aaccaccggc
     9601 ccgcaacgcg atccgctcga ccccaagaac ctgcggcaga cgaccctgcc actcggcagc
     9661 caggtgagct ccggcaccgc cttcaatccg gcgatgtcgg tgatcatcga cggcgcctat
     9721 tactcggaca acgtgcacgg cgaagccttc gagattctcg aggaagctga cgggttcgcg
     9781 atgggccacg gccacgacca cgagggcgaa gaacacggcc acggcggcct ggagcgcggc
     9841 ttcaacctcc gggaggtcga gttcgccttc tccggcaccg tcgatccgta tttcgatgca
     9901 tacgtgatgt tcgtgttcga cggcgacgag atcgagctcg aggaggcctt cctcacgacc
     9961 cgctcgctgc ccgcaggcct gcaggcgaag ttcggcaagt tcctcagcga cgtcggctac
    10021 atcaacaagc agcacccgca cagctgggac ttcttcgacg cgccgctggt cagcgagctg
    10081 ttgttcggcg atcacggcat ccaggaagtc ggtgtgcagt tgacctggct cgcacccacc
    10141 gaccagtacc tgcgcctcgg catcgaggcc ctgcagggcg aaaccgccgg catcgccaac
    10201 tacttcggcg cgcagggcga catcgagggc acggaacgca tcctcgagga cgcggcgggg
    10261 ccgcggctgt tcaccggctt cgccaagttt tccccgaacc tcggcttcga ccatgcgctg
    10321 cagctcggcg cctcgtacgg ctattccagc agcttccaga acagcgtcga gcacagcacc
    10381 cgttacgagg atcacgaggg cacggcgagc ttctggggcg tggacgccgt ctacaagtac
    10441 acgccgcccg gctcagtggg ctgcaagggc gccgtcaagt tgcagggcga gtacttctat
    10501 cggaagcgcg acatcgaccg gcaggacgtc tattttgcct gtcatccggc cggcatcgac
    10561 tgcgcggacc cggcggccca gtggcagatc ggcgacatca acaacgttga gtccttcagc
    10621 gagaagcagg acggctacta cctgcaggcg ctgtacggtc ttgcgccgcg ctggaacgtg
    10681 ggcgcgcgca tcgaccaggt cggcctcacc aacagcacgg ggcgcggtgc gggcgaggaa
    10741 tgggacgact cgaagcgtta ctcggcggtg ctgaacttca tgcctacaga gtactcgcgg
    10801 ctgcgcctgc agttcagccg tggcgacatc gcggtggaag gcgagcgcga gactttcaac
    10861 caggtcttcc tgcaattcca gatgagcctc ggcgtgcacg gcgcgcacac cttctagggg
    10921 cagcgacatg cgaaaacttt tactgacgct tgtgttgtgc ctcggcgcgc cgctgccggc
    10981 cgcggccctc caggtcacgg ccaccacgcc caacatgggc atgctggcgc gcaccgtcgg
    11041 tggccagcac gtcaaggtgg tgttgctggc gcccggcgac cgggacgtgc atcacctcga
    11101 ggcacggccc agcatgatgg tggcgttgcg gcgcgccgac ctgctggtgg cggtcggcgg
    11161 tgagctcgag gtgggctggc tgcctgcggc gctgcggggc gccggcaatc cggccatcct
    11221 gccggggcgc cccggctact tcgaggcggc ggcagcggtg acactgcttg gcgccggcgc
    11281 cgtggcgagc cgggcgggcg gtgacgtgca cccgatgggc aatccgcaca tctacttcga
    11341 tccgctgcgc atggcggcgg cggccgaggc gcttgccgag caactggccg tgctggacga
    11401 agccaatgcg gcggcctacg ctgcaaatgc gcgggccttc tcggcccgca tgcagcagga
    11461 ggtcgcggga tggcgggtgc gggtcgcgtc gtcgccgggc gcagttccct accacaagga
    11521 tgcggactac ctgttcgcgg ccctcgaggt gccggtgctc ggctacctcg agcccctgcc
    11581 gggcattccg ccgaccgcgc gccacatacg cgagctggtg gatgaactgc aaggcaagca
    11641 aggcgtgatc tttcacatgg tctacgagcc cgcggcaggg ccggagcgcc tggccaggga
    11701 actgggctgg ccggtattcc ggatgcgcaa caacgttccc gagcagggca cggcggacga
    11761 ctacgtggcc ctgatcgagc agtgggtcgc ccggctggag acgcgttgaa cactgccgtt
    11821 ccgctgctgg agctcatcga tgtccgggcc ggctattctc gacctgtagt cggcccggtc
    11881 tccttcgtcg tgcgcccggg cgaggtgctt ggcctccagg gcgccaacgg catcggcaag
    11941 accacggtca tcaaggccat cacgggcggc gccaggttgt tcggcggtga gctgcggcgc
    12001 accgccgggc tgaccatcac acatcactgg cagcgcccgg agcggccgcc ggagctgccg
    12061 ttgaccggcc tcgagatgct gcgcgtcacc agcgccgcca ccgcgtcatt gccaccgcgg
    12121 ctgcacgccc tgtgcgcgcg gtgcctcgac gagatgagtg gaggtgaatt ccagctgctg
    12181 catgcttggg cctgcctgac ggcgcccgtg cggctcgtgc tgctcgacga gccgaccaac
    12241 aacctggacc aggatgccat cgaactgctg ctcaccgaga tcggcaagcg tccgccgggc
    12301 cgcgccgtcc tgctggtcag ccatgaaacg gacttcctca ggcgcgcctg cgaccggatc
    12361 gtacctgtat gtccctgaac ctcgcctggg atccgctgtt tcgcctgccg ctggccacgg
    12421 cgctggtgct ggcggcgacc ctgccgctga tcggcgccgg cctgcggctg cgcgaacagt
    12481 ggttctcgag cctgggggtg gcacagatgg cggcggccgg cggcgtggcc ggtgcattgc
    12541 tgcacggccc gatcatgctg ttcgcgctgc tgggtgcggc gctcggcggc gcggctcgcg
    12601 ccctcgccgg acgcacccgc aacgagcact atgccgtgct gctgctcgcc ggctgggccg
    12661 cggtgatcct gctcgcgttg cagggtcacc acgccgatac caccgcactc cagctgctca
    12721 atggccagct gtatttcacg gcgctgcctc acctgctcgg cgcgattgca ttgcttggcg
    12781 cactgctggc ctgcggccgc tggctggcgc ggcggctgct gctcgcgcgg ctgtttcccg
    12841 atcattttgc ggccaacggc cgacccacct ggccgcatga gctgtgtttc gaggggctgc
    12901 tgatcgccgc agtggtgctg ggcatcagca cgatgggcgt aatggccacc ttcgccatga
    12961 tgttcctgcc cccctgggtg gctttccggc tggcacgcgg ctggccccgg gcgctgctgc
    13021 tcagcgccct catcggcctc gtcgcctgcc tggccggctt catgctcgcg ctggcgctcg
    13081 acctgccctt cggcgcggca ctcgtggcag tgctcctgct gtcagtcccc ctgcggctgg
    13141 cccccgtcag attcgggcgc caggccgact gagtcgtccg ccaggcaagc acagtcgcgt
    13201 gccgccggcg gcaggccacg cccggtggcg aggtaagtcg cgaaactgcc cagccagtgg
    13261 ctgcccgcat agtgctcgct gctgaccccc tccaggcccg cgcgtgcgtg ccgggcggcc
    13321 gttgcgcgca gcgtgatgcg gcgcaggtcg ccgggcggca aggccgcggc aatcccttcc
    13381 agcatccagg cgcggctcag gttcagcccg tcgatgtgcg ccagtttgcc gtccgctcgg
    13441 tccgtcacct gggcgaccgg caaccagccg tcgtcggtgt cttccgggat gtccggcagg
    13501 aaggccgcca gccagtcggc gaactccgcg ggcggcagca cacggcgcag caggtcggcc
    13561 tcggcgaggc atggcgagag gaagtcatgg ccggagggtt cgtaggcgag cgggcagtcc
    13621 cggtccgcca ggtagagacg gcgggacgtc ccaatgagca gttccgccag ctccgcatcg
    13681 ccggcggtgc gcgcccagtc cagcgccagc ccgaaggcga acgccgtctg gaagtgctcg
    13741 ccgccgcgaa tcgggtacgc cagcttcggt agccactcgc gcagtcgcga cgaggcgagc
    13801 gcctccagcg gcgcgagcgc caccgcccaa cgcgcggcgt cggggtcgtt ccattcgcgc
    13861 agttcggcgc ccagctgcag cagccaggcg aggccgtagg ggcgctcgaa gccttcgcgg
    13921 cccggcgcgg ccaggtactc gacttcggcg cgaatgccct cgggcgtcag gctgcgctcg
    13981 agcgcggcgc gcgcggcggg cgtgaacggg gcgtcgggct cgaaccgcgc cagtcgcgcc
    14041 agcaaccagt gcccgtgtac cgaggagtgc cagtcgaaac agccgtagaa caccggcgtg
    14101 agctcccgcg gcggcgccac gtcagcatcg ctcgccagca cgtgggcgat cttgttcggg
    14161 tactcccggt gcacgcatga cagcgccatg tccgcgaacc gcgccagcgc ggcgggatcg
    14221 atcacggtac cagccaccag catcagcgcg gcgacgctca ttgcggcggc agcatgcgac
    14281 cgagccgccg cccaccgagc aggtgcaggt gcaggtggta gacctcctgg ccgccgtgtg
    14341 aattgcagtt gatgatgagc cggtatccgt cgtcggcaat accctcggcg cgggcgaggt
    14401 cgcgggccac gcagaacatg tggccgatga ggcccgcatc ttcgtcatcg atgtcgttgg
    14461 ccgtcggaat cggcttgacc gggatgatca gcacgtgcac cggcgcctgc gggttgatgt
    14521 cgcgaaaggc gaccacgcgc tcgtcccggt aaaggaaatc ggcgggcagc tcaccggcga
    14581 tgatcttctc aaacagcgtg ggcatgggca gtactcctcg gcgcgcgatc ggctctggtc
    14641 cggccaagtt taggcagcga ccgggaccgg ccgcaacgcg ggccgcgcag gcttataatc
    14701 gtggctcgtg cctcgttctc ccggagatcc cgatatggcc acccgtctca cccgcttcgc
    14761 tgtcgcggtc gtggtccttg ccgcagcggg ctgcgccaac ctcgcggcgc tgcaggcgcc
    14821 agacgtgcag gtcacgagcc tgcagctgct ggagcccgcg ccgggctccc tggaacagcg
    14881 cttcgcggtc ggcctgcggc tgatcaaccc caataaccgc gccctggcgg tggacgggct
    14941 cgatttcgag ctcgagctga atgaccggcg actggcgcgc ggtgtcacca accgtgcctt
    15001 cgagctgccg cgcctcggcg aggccgagac cagcgtgatc gtcacgacct cggtgttcga
    15061 cgtgctgcgc caggccgtgg aactcggcag ccggcgcgaa tcggccatgg actaccggct
    15121 gcgcggccgc ctgcacctcg gcagcggctt cgtgcgcacc gtgcccttcg accaccgggg
    15181 caagctgacg ccctgacccc gcccgccgct caggcggccc gcaggcgcca cacgggacgg
    15241 tcgtgcagat cgatcaggcc gtgggcgcgc agccagtggc gcgcatcctc ggcatccccc
    15301 tcgaaccacg cccgtgccga accgaggcga aaggaatagc cccaggcatc catgtcggca
    15361 aacatccgca gccgcccgaa gcccggcaac tcgtcggcga gcagggcctg gaggtagcag
    15421 actccgttct cctcatcgta gtcgccgcct gcattggtat cgagctcggc gcgccgctcg
    15481 ggcgacatgc acacgaaatg gcaggcctcg tgcagcacgg aatgcagcgg ggtgtcggcg
    15541 cgcgcataca gcaccgcatc ccgcaggccg gcctcgcgtt caccccacca cgagccggga
    15601 atggactcac ccgccgggac gatctgcagc gccagcccga agcgcgccag cacttcgcgg
    15661 accgcatcct cgtggccgtc ggcgagcgtg aggacttcgc tcgcgttcaa tcgctcaggc
    15721 ccgccagccg ccgggtgatc actccggcga tctcacgcgc caggctctca cggagcaatt
    15781 cctcttcctg ctgcttgccg aggatgtccc gctcgctgta cgggaagatg cgtgtacgcg
    15841 cgatcgactg cggggcgacc acggtgccgg cgagcgtcgc cagcgtgtac tccgcctggt
    15901 agaagacttc gtactcggtg gggcgattct gcgccgacac cgacttcacg gtgcgcccga
    15961 agcgctccgt gacgatggac agcgtcgccg tggcgtcggt cgcgtcacgg gccagcgtga
    16021 cgcctgaggc cgtgagcgag cggcgcaact gcacggccag gtccgaagtc gggtccggga
    16081 cgtccaggtg gactgctgcc agctccggcg gatagcgcgt gacgccctgc aggtggtagc
    16141 cgcaggcggc gagtgcgagg ctaatgagga ccagcaggaa ggtgcctggg cgacgcatgg
    16201 ttcagaccac gatgttcacg agccggccgg gcacgatgat caccttgcgc accgggctgt
    16261 ccgccacgaa gcgggccacg ttctcctccg ccagcgcggc gctgcggatc gccgcctcgg
    16321 acgcatcccg ggcgatcctg atgcgaccgc gcagcttgcc cttgacctgc accacgagct
    16381 cgacctcttc gcgcaccagc gcggcgggat catgcgccgg ccacggttgg tcgacgacgg
    16441 cgcccgcacg gcccagcgca tgccacagcg catggcaggc gtgcggcacg atgggcgaga
    16501 gcatcagcgt gaccgcctcg aaggcctcct ggcgcagggc ccggtcctgc agctcccggc
    16561 cggcatgccg gccgagcgaa ttgagcagtt ccatcaccgc ggcgatcgcc gtgttgaagg
    16621 tgtaacggcg cccgacgtcg tcgctgacct tggcgatcgt cgcgtgcagc tggcgccgca
    16681 gctcgcgctg cgcctcgtcg agtgcgccgg cctcgagcgc cggcgccggg ccctcgccga
    16741 cgtgctcgtg caccatgcgc cacagccgct tcatgaagcg ctgcgcgccc tccacgccgg
    16801 aatccgacca ttccaggctc tgctcgggcg gcgccgcgaa catggtgaac aggcgcaccg
    16861 tgtcggcccc gtaccggtcg atcaatgcct gcgggtcgac gccgttgttc ttggacttgg
    16921 acatggtgcc cattccgccg agctcgaccg gcgcgccgtc ggccgtcagc gtcgcgctga
    16981 gcacccggcc cttgtcgtcg cgctgcacct cgacctcggc cgggttgtac cagctgcggc
    17041 cgcttttcgc gtcgtcacga tagaaggaat ccgccagcac catgccctgg gtgagcaggt
    17101 tggcgaaggg ctcgtcgcca tccaccaggc cctcatcgcg catcagcttg tgaaaaaagc
    17161 gcgcgtagag gaggtgcagg atggcatgct cgatgccgcc gatgtactgg tcgaccggca
    17221 gccagtagcg tgcgcggtcg tccagcatcg ctgcatcctg gtcggccgag cagaagcgcg
    17281 cgtagtacca ggaggattca aagaaagtat cgaaggtgtc ggtctcgcgc ctggccggcc
    17341 cgccgcactg cgggcagtcg acacgcacga actcgtccat ctgcttcaga ggcgagccgg
    17401 cgccggagag cgtgacgtcc tcgggcagcc gcaccggcaa ctggtcctcc ggcaccggca
    17461 ccgcgccgca cgacgggcag tcgatcatcg gtaccgggca gccccagtag cgttgccgcg
    17521 acacgctcca gtcacgcagc cggtagttca cgcggcggcg accgcgcccc tcgccctcga
    17581 gcttcattgc cagcgcctcg aaggcctccg ccgagctcat gccctcgaag ccgggcgaat
    17641 tcaccagcac gccgtactcc acgtacgccc cgtccgacag gtccgccgcg gacccgtcgg
    17701 ccggcgcgat gacctgcctg atcggcaggc cgtacttcgt ggcgaattcc cagtcgcgcc
    17761 ggtcgtggcc gggtaccgcc atgaccgcgc cggtgccgta acccatcagc acgaagttgg
    17821 cgacccacac cgggaccctg tcgcccgtca gcggatggac gacactgacc tcgagcggca
    17881 tgccgcgctt ctccatggtc tccagcgccg cctcggtggt gtgggcgctg cggcactcgg
    17941 cgaggaacgc cgccagttcc gtgttgccct gtgccgcgtg cacggccagc ggatgctcgg
    18001 cggctaccgc catgtaggtc acgccgaaca acgtgtccgg ccgcgtcgtg tagacggtga
    18061 gcgcccggta cccggccacc ggcgcgccat ccagcgcgaa gtccagttcg aggccctcgg
    18121 agcgcccgat ccagttcgcc tgcatggtgc gcacctgctc cggccagccc ggcagcgtat
    18181 ccagcgcctc cagcagctcg tcggcgtagg cggtgatctt caggaaccac tgcgggatct
    18241 cgcggcgttc caccagcgca ccggaacgcc agccgcgccc gtcgatgacc tgttcgttgg
    18301 ccagcaccgt ctggtcgacc gggtcccagt tgacggtcga ggaggacttg tagaccaggc
    18361 cctgctcgta cagtcgcagg aagaaccact gctcccagcg gtagtagtcc gggtcgcagg
    18421 tggccagctc gcggcgccag tcgtaggcga agccgaggct cctcaactgc gcccgcatgt
    18481 gcgcgatgtt ctcgcgcgtc cagcgcgccg gcggcacgcc gtgcttgatg gcggcgttct
    18541 ccgccggcag tccaaaggcg tcccagccca tcggctgcaa tacgttcttg ccctgcatgc
    18601 gctggtaacg gctgaccacg tcgccgatgg tgtagttgcg cacgtggccc atgtgcagtt
    18661 tcccgctcgg atagggaaac atgcagaggc agtagaactt ctccttcgag gggtcttcgc
    18721 cggcctcgaa acagccctgc tcgtcccagt aacgctgggc cgcgggctcg atcacttccg
    18781 gtttgtagat ggggtccatg gtccgcttcg ccacaatcaa tccgcgaaga ttaccgcccc
    18841 gccgtgttgc ggcgcaaagt ccccagcaag aacaggccca gggcgaccgc cgccagcggc
    18901 ccgtcgccgt agcgcagcca gggcgtcagg ccggtcatcg gcggcacctc ggcctcgatg
    18961 acctcggcga cgaactggcg cgagcgctgc gtcacccggc cgcggggatc gatcaccgcc
    19021 gtcacaccgt tgttggtcgc acgcagcatc cagcggccgg tctccatggc gcgcatgcgg
    19081 gcgatctgca ggtgctggtg cggcgcgcgc gtgtcgccga accaggcgtc gttgctgacg
    19141 ttgaccagca gttccgccgc aggaaagaac acccgctgtt gcgcgccgaa ggcgtcctcg
    19201 taacagatgg tcggcgcgac cttgacgccg gccagggtca tcggcggcgg atcggcgcgg
    19261 ccgggagcga agtcggtata gggcaggttc atgagccgca tccagcgacg tacgaaggcc
    19321 ggcaccggga agtattcgcc aaagggcacc agctggcgct tgcgataaat ctccggctgc
    19381 ccgcccagcg agaccagcac gttgtagtac tggccgctct ggcggtcgcg ctccaggatg
    19441 ccgagcagga tctcgctgcc ggcggcgcgg gcttcttcgt cgagcgcctc gatgaaatcc
    19501 atctcgagat gcagcaacgc ggggatggcg gcctcgggcc agatcaccag gtctgcgccc
    19561 cagtgcggcc gtgtcatgtc gaggtacagc tggcgggtcg gctccagcga cgcctccagc
    19621 cacttctcgt cctggccgat cgcgccttgc accatcacgg cgcgcagcgg ctcgccgacg
    19681 ggcgcggtcc atgacttgcc gccggcatgc cccgcggccg caaaaaccac gaacagggtg
    19741 gcgaccgcca gtgcgcggcc cgcgaggcct tccaccagca ccgcggccag cgcgccggcg
    19801 ccgagcgcgc aaacccagct caggccgtag acgccaccca ggggggccag ggcgccgagc
    19861 accgagtcgg tctggctgta gcccaggctg agccacggaa aaccgctcag gaaccagccg
    19921 cgcagccatt cgacggccag ccatcccgcc ggccggcagc cagcgcgcgc ccagccatcc
    19981 gagcaaggcg taatagacgc ccatgatcgc cgccagcccg aaagtcagga acgccgccag
    20041 ggccagcggc gccccgccga actcgcgcac cgagacaaag gtccaccagg taccggtcag
    20101 gaaggcggca aagccgtacc cccagcccag tcgcgccgcg cgccgcggcg ttgccccgcg
    20161 ccaggcgagg aacagcaccg ccggggagat cagcgccagc ggccagtaat tccagggtgc
    20221 aaaggcaaac ggcagggcca ggccggcagc gagtgccgcg atgtcggcgg ggccgaagcg
    20281 ccgcccgctg cgcggcaaag cggagccgac cgccatccgc tcagccggcg gcgcggcgca
    20341 ggcgcaccgc gtgcacgcga cggcgatcgg cgcgcagcac ggtgaaacgc atgccgtcga
    20401 gcaccagcga ctcgcccttg cgcggcagcc ggccgagctc ggtcatcacc agcccgccga
    20461 ccgtgtcgta gtcggcgtcg ctgaaattcg tcccgagctc ctcgttgagg tcctcgatgc
    20521 gagtcaaggc atcgactcgc caggcgtcct gctgctccgg cacgatcatg acctcctcgt
    20581 cagggtcgtg ctcgtcgtcg atctcgccga cgatctgctc cagcacgtcc tcgatggtga
    20641 gcagtccgga cacgccgcca tactcgtcga ccacgatggc catgtggttg tggctgacgc
    20701 ggaactcctt caacagcacg ttgaggcgct tgctctcggg aatgaagacg gccgggcgca
    20761 ggcactcctt gatgtggaag cgctcgcgct cgttctccgc atagaagcgc agcaggtcct
    20821 tggccagcag tatgccgatc acctcgtcgc ggtcgtccgc gatcaccggg aagcgggaat
    20881 ggccggaatc gatgatgatc tggatcattt cctgcgggct ggcattgcgc tcgaccacca
    20941 ccatctggcc gcgcgggatc atgatgtcgc gcacctggat ggtggcgacc tcgaggacgc
    21001 cctcgagcat gctgaactcg tctgcatcca ggatgcggct gcgccgggcc tggtcgagca
    21061 tctcgatcag cgcctggcgg tcccggggcg cggctcggaa ggcgccggcc cagcgggcca
    21121 gcaggccgga ccggcgcccg cgggtactcg gaggtttgtc gtcagtcatc gttcggttca
    21181 cgctcccggt aggggttggg ccagccgagc tcggcgagca ggtccgcctc cagcgcctcc
    21241 atggtcgccg cctgctcgtc ggtctggtgg tcgaagccca gcaggtgcag cgtaccgtga
    21301 atcaccatgt gggcccaatg cgcgtccagc ggcttggcct gctcggtggc ctcggcctcc
    21361 accacctcgc gacatatcac caggtcgccg agcagcggca ggccgagctc ggccggcagc
    21421 tcggccggga aggcgagcac gttggtcggc cggtccttgc cgcgataacg ccggttgagc
    21481 gcccgactct cggcggcgtc gacgatgcga atggacagct cggcgtcgtc ccggcggccg
    21541 gccagcgcgg agcgcgccca gtgccgcagc ttggccacgc cgggcaggcc ggtgcgcgtc
    21601 ccgccgacct ggacgtcgat gcgcaggctc actgttgctc gtccccctgg tggcgctggt
    21661 aggcctccac aatgcgttgc accagcggat gtcgcaccac gtccctggcc gtgaaccagg
    21721 taaaggccac gccctccacg cccttgagga cgtcgctggc gtgcttgagg ccggacagct
    21781 tgctggccgg caggtccgtc tgggtgacgt cgccggtgac caccgcgcgc gagccgaagc
    21841 cgatgcgcgt caggaacatc ttcatctgct cgacggtgga gttctgcgcc tcatcgagga
    21901 tgacgaaggc ctcgttgagc gtcctgccgc gcatgaaggc cagcggcgca atctcgatga
    21961 cgtgccgctc gatcagcttg ccgacccgct cgaagccgag catttcgtag agcgcgtcgt
    22021 acatcggccg caggtagggg tcgaccttct gcgacatgtc gccgggcagg aagccgaggc
    22081 gctcgcctgc ttccaccgcc ggacgcacca gcacgatccg ccgcacggcg tcgcgctcca
    22141 gcgcttcgac cgcgctggcc actgccagat aggtcttgcc ggtgccggcc gggccgatgc
    22201 cgaaacacag gtcgttgtcg cgcatggact ggatgtagcg tgcctggttc gggccgcgcc
    22261 cgcggatgac gccgcggcgc atgcgcagct cgcccggcgc accgccaccc ggctcctcgt
    22321 acaggccggc ctcctgcagc caggcgtgca cgcgctgcgg gttgatgctc tcggtggcgg
    22381 cctgctcgaa cagcgccacc agcagccgct cggtcgcatc caccgcgcgc tcggggccct
    22441 cgatctggaa gcagttgccg cgcgagccga tcgacacgcc gaggcggcgc tcgatctgct
    22501 tgaggtgctc gtcgaactgg ccgcagagat tggcaagccg ctggttgtcg gcgggctcga
    22561 ggaccaggtc cctgactagg ttggcgttca aatggttggg atacctcagg cggaacgggc
    22621 cagcgcgccg tcgacgccaa ccaccctgcc gcgcagggaa tttggcagcg cctcggtgat
    22681 cagcacgtcg gcgaactggc cgacgagcgc cggctcggcg tcgaagttca cccagcgcat
    22741 gttgccggtg cgtccggcga gctggcgggg attcttcttc gaggggcgct cgacgagcac
    22801 gcgctcgacg ccgccgacca tcgcgcggct gatctgcgcg gcgcgctggt tgatgcgatg
    22861 ctggagcaac gccaggcggc gctgcttctc ggccatgtcg acgtcgtccg gcagcgcggc
    22921 ggccggggtg cccggtcgcc ggctgtagat gaagctgaag gactggtcga agccaatgtc
    22981 gtcgatcagc tgcagcgtct gctcgaagtc gcgctcggtc tcgcccggga aaccgacgat
    23041 gaaatcggag ctgatgctga tccctgggcg cgcctcgcgc acgcgccgga tcttctggcg
    23101 gtactcgagc gccgtatggc cccgcttcat cagggcgagc acgcggtccg agccgctctg
    23161 caccggcagg tgcaggaacg gcgccagctc cggcacctcg gcataggcgg ccaccaggct
    23221 gtcggtgaac tccacggggt gcgaagtggt gaaacggatg cggtcgacgc cgtccacggc
    23281 cgccacgaaa cggatcagcg cggcgaggtc ggcatgggtg ccgtcgtgca tgacgccccg
    23341 gtaggcattg acgttctggc caagcaggtt gatctcgcgc acgccctgcg ccgcgagctg
    23401 cgccacctcg acgatgacgt cgtcgaacgg ccggctgacc tcctcgccgc gcgtgtaggg
    23461 caccacgcag aagctgcagt acttgctgca gccctccatg accgaaacga aggccgtggg
    23521 accctcggca cgcggcgccg gcagccggtc gaatttctcg atttccggga aggagatgtc
    23581 gacggccgca cggccgcggc cgcgcacttc ttcgagcatc gccggcaggc ggtgcagggt
    23641 ctgcgggccg aacacgatgt ccacgaaggg cgcgcggccc aggatggcct cgccctcctg
    23701 gctggcgacg cagccgccca caccgatcac cactccgggg cgcgcctctt tgagttcgcg
    23761 ccagcgcccg agcagcgaga agactttttc ctgggccttc tcgcggatcg agcaggtgtt
    23821 cagcagcagg atgtcagcct cctcggggct ggccgccgcc tcgaggccat gtgaggcccg
    23881 cagcacgtcc cccatcttcg acgaatcgta ctcgttcatc tggcacccga aggtcttgat
    23941 gaacagcttg ccgcccggct tgtcctggct catcggcgcg gccctagaag gggatgtcgt
    24001 cctcaaaggg ctcttccttg cgggcgccgc ccgaagaacg gccgccggag ggctgctgac
    24061 ggtaatcgtc gtcgccgccc gccggcgcgc cggcgccggg ccggccgccg agcatctgca
    24121 tgtcgcgggc gatgatctcg gtggtgtacc ggtcgttgcc gtccttgtcc tgccacttgt
    24181 tggtgcggat ctgcccctcg atgtagacct gcgagccctt gcgcagatac tcgcgcgcga
    24241 cttccgcgag ccggtcgaac atcacgacgc gatgccactc ggtctcctcg acgagatcgc
    24301 cgctctgccg gtccttgcgt ttctcggtgg tcgcaacccg gatgttggtg acggtcaggc
    24361 cgcctggagt cgtccttgat tccgggtcgg cgccgaggt
//
LOCUS       DAOMPG010000006        23476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_928_length_23476_cov_4.089791, whole genome shotgun sequence.
ACCESSION   DAOMPG010000006 DAOMPG010000000
VERSION     DAOMPG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 23476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23476
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_23476_cov_4.089791"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="PLS34_00720"
     CDS             <1..1947
                     /locus_tag="PLS34_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014108320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP86026.1"
                     /translation="ILDKNQSHRLSKEIQAITEQASAEFGATGEWHITGLPVAEDTFG
                     YEMFVQMGISAPLAGVAIFLLLLMFFRSLPLIVAPMIVAMVTVIATMGLLIGMGFTVH
                     IMSSMIPIFLMPIAVVDSVHIMSEFADRYRPGRGQKETMREVVGHLFRPMLFTSVTST
                     VGFASLALTPIPPVQVFGAFVAFGIMLAFLLTIVFVPAWVSRMKPASLEKMQLALHKR
                     EGDSPLARVLRGTGAFSLARGRLLLLGFAAVFAFSVVGITRIQINDNPTRWFKSDHRI
                     RVADQVLNHHFAGTYDAWLVLDRTAETLTAEEYNERISALEDRLFEAEGDELAALEAQ
                     IAELEEAASQARYFQRPDILRWIATLQADLESSGLVGKSNALPDLVKTVYRELAGGDP
                     ADFRIPDSVPGVAQALLQFQSSHRPQDLWHMVTPDFRRAVVWLQLTSGDNQDMTAVIR
                     HVDAFLESHPAPEGMAVRWAGKTYLNVVWQDAMVKGMVSSLAGAFIAVLVMMIVLFRS
                     FGYGLLAMLPLTITITFIYGLIGWTGKDYDMPIAVLSALTLGLSIDFAIHFIERFRES
                     LAQRGDFRAAIAEMFEEPARAISRNAIVIAVGFTPLLLAPLVPYITVGVFLATIMAVS
                     AIVTLVMLPAVLQPILGRTPQGAA"
     gene            1968..2771
                     /locus_tag="PLS34_00725"
     CDS             1968..2771
                     /locus_tag="PLS34_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRP86027.1"
                     /translation="MNHLLVRLAAIGLALPAVVHAQALTDVGEIIDRANLAAYYAGDD
                     GRSEARMAIEDAQGRTQLRQFTVLRRDRAEGGDQDFMVFFSRPSDVRGTVFLVAKKVD
                     RDDDRWLYLPGLDLVRRISAGDKRTSFVGSHFFYEDVSGRHPQADEHTLVETNDQHYV
                     IESKPKNPGTVEFARYVTRIDRGTFLPMKTDYYDARDRVYRVMEVLGVQDIGGHPTVT
                     RSQISDPVNGGRTLMEFRYTEYDVGLPADIFSERYLRTPPVDWLRRGGE"
     gene            2776..4098
                     /locus_tag="PLS34_00730"
     CDS             2776..4098
                     /locus_tag="PLS34_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86028.1"
                     /translation="MRAGVLLVLLAGLLMATDTSAWAQDDPWGGTGDDPFAQFEEDDD
                     WEDPWATGRGGLSWHGFVEGGLGGRWQSVENLDRLTLGELRLRAETSYDHGSFRIDFK
                     GDLAWDQVVEDLDGQLRELAVSFRPGTSMDAKIGRQVLTWGTGDLLFLNDLFPKDFVS
                     FFAGRDEEYLKSPSDTVRLSLFSPVVNVDLAYTPRFTPDEYLTGERFTFWNPALGEAA
                     APARPIKADEPSSGEFALRMYRTVGATEYALYGYSGRWKQPVGLDASGQLRFPRLRSW
                     GGSVRTPFHRGLLNVETAYYDSRDDRDGDDPATPNSELRLLLGYEQELVRNFTAGTQL
                     YAERLMHHDRLVANSPDPGREREQWRTVATLRLSHRAMQDKLTSSLFMFVSPSDEDFY
                     LRPSIAWRRDDHWLLAAGFNLFGGSEQHTFFGQLEDNSNGWLRVRYHY"
     gene            4117..4359
                     /locus_tag="PLS34_00735"
     CDS             4117..4359
                     /locus_tag="PLS34_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011788872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRP86029.1"
                     /translation="MTVERSVTAFAGFMVLLSLALWWLVHPGFIWLTVFVGFNLLQSS
                     FTGFCPAAIVFRKLGIKTEAEVARGATGGRGVGAAA"
     gene            4367..5929
                     /locus_tag="PLS34_00740"
     CDS             4367..5929
                     /locus_tag="PLS34_00740"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP86030.1"
                     /translation="MQGSQRGMIEALSEPGAYPPPVDKVEYVETHISWVFLAGDYAYK
                     FKKPLDLGFLDFTTRKRRLRFCREELRLNGRLAPEIYLDVVGAQPTRDGWRVGELTAD
                     AEPAVRMRRFPAEARLDRVLEAGRLDAEVLENFAGELAHFQRSLPPARPGDGFGDAAT
                     VTRPALENFQALPVEPLDGPGRQRLDELRQWTQARAAVLAERLTARLASGFIREGHGD
                     LHLGNLVMLDGRVAAFDGIEFDPALRWIDLQSEVAFLLMDLESRGHGALGWRFYNAWL
                     AALGDYDGIGLLNWYLVYRHMVRAKIDGIRLSQGELASSEATRLAARQAAHIELATRH
                     ARPPTPSLVVMHGLSGAGKSHLARRLAPRLPAAWARSDLERKRLHGLDPGSAASAAVG
                     DGLYSAGATERTYARLAEIARGALRSGVSMVVDAAFLESRRREQFIDLALECGGHPVV
                     VACEAPAELLRKRVAARRNDPSDADIAVLESQLARPIEIGPREAPYRVAVDTSGEPDI
                     DALLNRLRSTRA"
     gene            complement(5997..6665)
                     /locus_tag="PLS34_00745"
     CDS             complement(5997..6665)
                     /locus_tag="PLS34_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86031.1"
                     /translation="MRAAPIALGCWLLPTAAVLITYTVSASQGQVPTCFPFLEGCTSI
                     SGAARRDPSIHIFRAMMLPTATLIGGYWLLVRAWLGHLGETGRARDWITVLGLVGALF
                     MVLYVVFLGTDGRAYELMRRYGVTVYFSFTALAQLVLAARLNRLPGEGQVRVGHGVIH
                     AKKAFGATMLLLGLANIPLANYFPELQIDNSIAWNFALLMHGYFGLTAIAWHRAGFHV
                     ATRM"
     gene            complement(6665..6895)
                     /locus_tag="PLS34_00750"
     CDS             complement(6665..6895)
                     /locus_tag="PLS34_00750"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HRP86032.1"
                     /translation="MASGQVGNTAELAAAIDVSPAMVEAMVAELERRGLLQRAGECGT
                     ACAGCPSESACDPALQGTAWMLTAAGRRYAEH"
     gene            complement(6912..8966)
                     /gene="feoB"
                     /locus_tag="PLS34_00755"
     CDS             complement(6912..8966)
                     /gene="feoB"
                     /locus_tag="PLS34_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRP86033.1"
                     /translation="MNANLQPLAPPSSGARPAESRRLVIGLAGQPNVGKSTVFSILTG
                     LTAEVGNWPGRTVERREGSARIAGRDFRVVDLPGTYSLTSNSEEERIAREFILRERPD
                     VIVMLADAAALERNLYLLAELLLLPIPVVLGVNMVDVARREGIEFDAHVLEAALGLPV
                     VPMVASRNEGVAEVLAAAAAVADGKQAFAPDRPEIAAAHREVFQKVLALLAPSAPAGM
                     PPDWAALKLLEGDREITVTAQQWLPAETWHEVHELLREHEDAVLDVAGARYEWIGRMV
                     RAAVRRPRLGQVSFTDRFDRIATHPGWGLALLLSVFGLVFWLTFSVAGPVQEWLEEAV
                     VARISTALAGVLAGAPAWLSGILLDGMLAGAGVVLTFVPVLFVFFIALGFLEDTGYQS
                     RMAYVMDRFMHRIGLHGKSFLPLFLGFGCNVPAVMGSRVIESRSARLLTIMLTPFVPC
                     SARLLVLAFLVPLFFPVYPALVAWGLVVGNLAVLVLIGLLLDRFAFRRERLAFVMEMP
                     LYHVPNPRTILVFAATNVWTFVKRAATIIVLASMIIWLLVSYPGAHVNDSYLAAFGRA
                     LEPVGALMGLDWRLIVATLSAFVAKENSIAALGIIFGTEGQALGTTLAALVTPASALA
                     FLVVNMLFIPCVATVAAVRQETRSWGWTAASTGLMLASALLFGTLVYQAAVMAGLGGT
                     VV"
     gene            complement(8911..9195)
                     /locus_tag="PLS34_00760"
     CDS             complement(8911..9195)
                     /locus_tag="PLS34_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRP86034.1"
                     /translation="MASAEPIVLSELPDGFCGIVARVTGGRELTARLAAMGLTAGTEI
                     VVLQNRGFGPMLARVRDTRIALGRIQAGLVTVYECQSAASRPAVERGAPG"
     gene            9358..9819
                     /locus_tag="PLS34_00765"
     CDS             9358..9819
                     /locus_tag="PLS34_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86035.1"
                     /translation="MPLKIRMFFAQSMIASLLAFVGAAAYAAEPGLAVLMERMQTYSH
                     KLQLSVAARNAPLAGFYLHELEETAESIIENIASYDEQPVGELTRQMFMPALELLEDA
                     VDDADWSAGDQRFTAMLEACNGCHVASGHGQIRIVPASGNPFAQDFSVQPD"
     gene            complement(9831..11045)
                     /locus_tag="PLS34_00770"
     CDS             complement(9831..11045)
                     /locus_tag="PLS34_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HRP86036.1"
                     /translation="MEGLVAGVFVAVYAGMLLGRVPSLQLDRTGVALLGAIVLVAAEA
                     VSLDAAVAAIDGPTMALLFGFMVLSAQLRQSGFFARVTWWLASRDTGPALLLGALVFG
                     AGALSAVFSNDIVCLAATPVLLEACRRRGLDPVPYLVGLACAANVGSAATLVGNPQNM
                     LIGEALGLEFAWYTLYALPPVVGGLALTWGVIALLTRGRWRQGEPGVPATPPEVDGWQ
                     TGKGLVVAAALLVLFLWAPWPRELLALAAAGILLTSRRMHSRQTLGLVDWQLLVLFAG
                     LFIVNHAFQATGLSAAALDWLAARGLDLGAPLALFVATPVLSNLVSNVPAVMLLLPAA
                     QGESAGLILGLASGLAGNLLIIGSIANIIVVGLASAGGVTISWRRHAALGVPVTLATL
                     ALAGAWLWALGA"
     gene            11178..11783
                     /locus_tag="PLS34_00775"
     CDS             11178..11783
                     /locus_tag="PLS34_00775"
                     /inference="COORDINATES: protein motif:HMM:NF037675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP86037.1"
                     /translation="MSSNPTTRDPDLTRERILAAAHALFVAKGFAAVSMREIANRSGV
                     TKSLIHHHFGSKEALWELVKERAIAAYAENQAAELREASEPDADLLRRGIANYFRFLR
                     DNPGVVRLFAWTHLDNDPSCGRLDAELVQLGAERIRQAQARGLLRPDVNPAHVVTMFV
                     NACTQWFSARAHHSQWPGVGGDQEYLDDFLKIFMEGLLPRP"
     assembly_gap    11946..11955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11956..12904
                     /locus_tag="PLS34_00780"
     CDS             <11956..12904
                     /locus_tag="PLS34_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP86038.1"
                     /translation="AEVGLSAPVRELRLPGVLRAAQRAEPAFLHPGHLAERFVARGDR
                     VVAGQRLASLQNPSLGPAVASAEARVRELDERLIQLDADHARAQELHARGLASEELLD
                     RTLAERNATRQARAQALAAVAQARDQLAEAVLRAPFEATVGDLLVEPGDYVQPGQPVL
                     VLAGEDGLELEVQMPEGLARSLAPGTVVEIRAVGTGRRIQGRVREVGVARGGRPAPAV
                     IALPATEGWEPGISVHAAITLAEDPGLTVPLGAVVDPGTGQTRLFRIVNDRAELVPVQ
                     VGRLVGERVIVRGELAAGERVVIAGHQQLLDGEAVRVLP"
     gene            12922..15945
                     /locus_tag="PLS34_00785"
     CDS             12922..15945
                     /locus_tag="PLS34_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP86039.1"
                     /translation="MERRRLILAAVATLSLIGLFAWLEMNRQEDPFFPYRYGQVLVSW
                     PGADAAQVERLILNPLEEEIGQIEEVNELRGTTRLGFAQLIIGMHQHVYDTDAVWERI
                     RVAVARAERKFPDGTGQPEVLDRMMDSEGIVLAVTGSDDLLVLNDAARALRRDLFRMD
                     DIARVELLADPVEQLVIGLDPSVGANLEIDAASIAAQLAARNVTMPGGSMTRNGRSLA
                     LRPQTEFRDLEELGETPIRLPSGALLPLREIASLRLAPADPPTERMWFNGRAAVGLGI
                     VIPENRLNSVRFGAQLRALVDELRPDYAPLEIHEMFYQPQWVEDRLAELARSLLTGVA
                     VVMLVLLAFMGLRLGLTVAAVLPLVTLSSLAIYAMGGGVLHQMAVAGLVIALGMLVDN
                     AIVMVENIQWHLDQGRAPREAAVLSVRELAGPLAAATGTTLAAFTPLLLASGDTGDFT
                     RGIPILVMLTLAVSYVYAVFATPVMATLALRPKATAAADRTARLGERLGSIAVARPWS
                     VVAVAAALVAVGVLLSGLLPRDFFPSTDRNQLVVDLRFSEGTELQHTALQTNAIAGEI
                     AGLPTVTDVHVFAGFSGPRFYYNLIKIPRTPHLGRIVVVAERDRDLPALMHWVRQRAG
                     EILPDAHVVAARLGQGPPLDAPLEIRVFSEDPAQLVAVAEQVMKLLRDTPGAVDVRHS
                     LGTGLATLSLDIDDAAAARLGLARSDVATLLSAVSLGKEFSTWRARREPLPMLLRSPE
                     GRAFPVEALEGLPLALPGGGSVPLGHVVNIRAEWQPAVIRHWDMQRMTQVLAETADGY
                     TYDQVLSALAPRIDKLELPPGVRLVYGGAAAEAGEANSALFQMLPIGVLLLLVFLLYQ
                     FNSFRLLGIVLVTVPLAVVGVTPGLLLAQQPFSFTAILGVVALVGIVVNNAIVLIDLI
                     RRLETDGMDAAAAIVAAVARRTRPILMTTATTVAGLLPLTLTRSTLWPPLAWAIISGL
                     VASTLLTLVVIPALYKLLMTAKERPTAPAQAPGSV"
     gene            complement(15924..17054)
                     /locus_tag="PLS34_00790"
     CDS             complement(15924..17054)
                     /locus_tag="PLS34_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRP86040.1"
                     /translation="MLEQYDQATERYALVSALLSLHEEAATLDEQGVGQRAVELAEQL
                     TESRSAYLHVVEKGVDIRLACWSARTLLTCAIESESHYSLDRAGIWADCVREGRPVVH
                     NDLAREHYRRGLPEGHSPLARHLAVPVLEKGSPVMVLGVGNKRSPYTDEDVDRVRLLA
                     ESAWGLLRRKAHEQREARHREFLEANMRGTIDVVNTIVEMRDPYTAGHERRVAHLACA
                     IAVDLGLDEETRDRIGLAAQVHDVGKLAVPHEVLTKPGRLTPMEMEVVKSHSRQGYEI
                     LRRSHFSWPLADIVLQHHERYDGSGYPDGRRGEEILLEARIIGVADVVEAMSSPRPHR
                     PRRPIESALAEIEAGAGTRYDPRVAAACLALFRAKGYTLPGA"
     gene            complement(17071..20754)
                     /locus_tag="PLS34_00795"
     CDS             complement(17071..20754)
                     /locus_tag="PLS34_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP86041.1"
                     /translation="MDSDGTPPGGPRSITVPRRIALGIALVYALLGSLWIIASDRLLP
                     DLGVDVDDLMWLGTAKGLLFVYVTSAVLYVLMRVTPREPEEGELPQARGGLLSVVFFC
                     LAGLILLVGYQVQRQHLQSIEERSYSALRSTAFIKAQRVGEWFGDQVGAVAALGHIYA
                     EGAAGLGTFEESPAAHAELLARIAPTARLLGLDDVALADAGGRLLPAGGGTPDADLLA
                     RAIRDAADGQIGFSGVAPADGGTMSALLTYRLASRNGATDGPSWLVATIDPGPRLVSI
                     LQRWPLAGISGETSLASLGPDGELVFLPGFEAPVHGGRLLVFPTRTQEPGSDYAPRGE
                     HGIIEGSLPDGRSVLLTMARVSGVHWIVVGAIDRDDVYAGVFKLLRNTALVVLVAVLI
                     AGWLILVLFRQQQLLNRAMLESVKRERDVLNQHFSYLTRFANDIVLLMDADGRIVNCN
                     DRALAAFGYSRDEFIGRDTLELRPPALVAAGRRQLGIARQEGGLVYETWHRRKDGSEF
                     PVEASVRSFEVDGETFIQGILRDITERTRAEEAVRRSEARFRNTFDRTAVGLAHVSTD
                     GRFLLVNPRFAELLGYSSEELEGMTVWQISQPEDLPRELSLGKAVMAGQREDFSIEKR
                     YRRKDGSIFWAQLTASAVRQENGQVEYLVGVIEDIGPRKRLQAQLKRRTKLYRTLSET
                     NRAIARLPELEALLAEACRIAVTHASLPLAAVARPGETGPCLAASAGPEVEAFASQLL
                     SAPAPGDVFAFPRGDGAQPGIIVVNDVEAAPVGAGTRLACLRRGVRAFASFPLGSAPI
                     PEARFAVFSRETGYFEGDTLDLLSDMARDLTLAMDRATERGARERAEARFREVARRMT
                     ALIDAAPVPVFGLDAEGRVLDVWNPAAERVFGWRRDEVLGRRLPIVLEDDAHHAEFDA
                     MQRQVLGGDELLGLEARCRRRDGSVGHVSVSASRITMADGDFGVMVVAEDITARHQAA
                     EALRIARDELEKHVAERTAELAAARDRAEEADRIKTEFLANISHELRTPLNSIIGFSS
                     LLLSGAPGAVNDEQEKQLTIVRRAGERLLALIEDILDISRLQASDTRLPCEPTPLRDM
                     LLRAADTMRPQAIAKGLAIHTEIEYCAALAERRRLEQVVNNLVSNAVKYTDHGEVIVR
                     CRRTDGQVEIAVEDTGRGIPAAELERLFVPFAQLGGGEAGRPGAGLGLAISRRLVEAM
                     GGSISVDSEPGRGSVFIVRLDAA"
     gene            complement(20897..20973)
                     /locus_tag="PLS34_00800"
     tRNA            complement(20897..20973)
                     /locus_tag="PLS34_00800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20937..20939),aa:Met,seq:cat)
     gene            21158..23440
                     /locus_tag="PLS34_00805"
     CDS             21158..23440
                     /locus_tag="PLS34_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HRP86042.1"
                     /translation="MYDTKRLWWTLGIILVVSFGILGFMGGEIYRTMPPVPDAYVSAD
                     GEILYTRDDIERGRQVWQTTGGHQQGSIWGHGAYVAPDWSADWLHREAVALLDVYAQQ
                     DFGRGFDELDEGPQAALRQRLKSEMRNNTFDPASNRVTVSAERARAIREVAAHYEGLF
                     GDDPELAQLREAYAMMQFPVPDAERRQLLGAFVFWSSWAAATNRPGDDITYTNNWPHE
                     QLIDNTPTGASFMWTFVSILILLAGVGALAWHYAMVHGHEPPPEVPAIDPLHNLKPTP
                     SMRATVKYFWVVTALFGLQVVLGGITAHYAVEGQGFYGVNLSEVLPYVITRTWHTQLA
                     VLWIATAWLATGLYIAPAISGHEPKFQRIGVNFLFVCLLVIVLGSMAGQWLGVMQKLG
                     LDINFWFGHQGWEYVDLGRFWQIFLFIGLMLWLTLVGRALWPALKTKDEGRPLVILLF
                     LSTVAIGLFYGAGLMWGKHTHLAIVEYWRWWVVHLWVEGFFEVFATAVIAFLFTKLGL
                     VRTTSAVTAVLFATIVFLTGGVLGTFHHLYFTGTPTAVLAIGASFSALEVVPLVLIGF
                     EAYENWKASKYRGWVATYKWPIMFFISVAFWNLVGAGLFGFLINPPLALYYMQGLNTT
                     PLHGHTALFGVYGMLGIGLMLFCLRGLTYRQAWNEKLLSVSFWSLNLGLAAMALFSLL
                     PLGLMQVWASVTEGFWYARSAEFLQQPLMQTLVWLRVPGDVIFSVGVAALAWFVFRLF
                     VGPRETVPARSGTTVAEARE"
BASE COUNT         3755 a         8186 c         7833 g         3692 t
ORIGIN      
        1 atactggaca agaaccagag ccaccgcctg tcgaaggaaa tccaggccat taccgagcag
       61 gcctccgccg agttcggcgc caccggcgag tggcacatca ccggcctgcc ggtggccgag
      121 gacacctttg gctacgagat gttcgtgcag atgggcatct cggcgccgct ggccggcgtc
      181 gcgatcttcc tgctgctgct gatgttcttc cgcagcctgc cgcttatcgt cgcgcccatg
      241 atcgtagcga tggtcacggt gatcgccacc atgggcctgc tcatcggcat gggcttcacc
      301 gtgcacatca tgagctccat gatcccgatt ttccttatgc cgatcgcggt ggtcgactcc
      361 gtgcacatca tgtcggagtt cgccgaccgc tatcggccgg gccggggcca gaaggaaacg
      421 atgcgtgagg tggtcggaca cctgttccgc cccatgctgt tcacctcggt gacctccacg
      481 gtgggcttcg cctcgctggc gctgacgccg attccgcccg tgcaggtgtt cggcgccttc
      541 gtcgccttcg gcatcatgct cgctttcctt ttgaccatcg tgttcgtgcc ggcctgggtg
      601 tcgcgcatga agccggccag cctggagaag atgcagcttg ctttacacaa gcgcgagggc
      661 gactcgccgc tggctcgagt actgcgtggc acgggcgcgt tctcgctggc gcgcggccgc
      721 ctgctgctgc tcggctttgc cgcggtgttc gcgttcagcg tcgtcggcat tacccggatc
      781 cagatcaacg acaaccccac ccgctggttc aagtccgacc atcgcattcg cgtggccgac
      841 caggtgctga accaccactt cgccggcacc tacgatgcgt ggctggtact ggatcgcacc
      901 gccgagacgc tcaccgctga ggaatacaac gagcggatct cggcgctgga ggaccgcctg
      961 ttcgaggccg agggcgatga actggccgcg ctcgaagcgc agatcgccga actggaggaa
     1021 gcggccagcc aggcgcgcta cttccagcgg cccgacatcc tgcgctggat cgccacgctg
     1081 caggcggacc tcgaatcaag cgggctggtg ggcaagagca atgccctgcc ggacctggtg
     1141 aaaaccgtgt accgggaact ggcgggcggc gacccggccg acttccgcat cccggacagt
     1201 gtgcccggag tcgcccaggc tttgctgcag ttccagtcct cgcaccggcc gcaggatctt
     1261 tggcacatgg tgacgcccga cttccggcgc gcggtcgtct ggctgcagct caccagcggc
     1321 gacaaccagg acatgacggc agtgatccgg cacgtcgacg cgttcctcga gtcgcacccg
     1381 gcgcctgagg gcatggcggt gcgctgggcc ggcaagacct atctcaacgt cgtctggcag
     1441 gacgccatgg tgaagggcat ggtcagcagc ctcgccggcg ccttcatcgc cgtgctggtg
     1501 atgatgatcg tgctgttccg ctctttcggc tacggcctgc tggcgatgct gccgctgacc
     1561 atcacgatta ccttcatcta cgggctgatc ggctggaccg gcaaggacta cgacatgccg
     1621 atcgcggtgt tgtcggcact gacgctgggc ctgtcgatcg acttcgccat ccatttcatc
     1681 gagcgcttcc gcgaatcgct cgcgcagcgc ggcgacttcc gtgcggccat cgcggagatg
     1741 ttcgaggagc cggcacgcgc catcagccgc aacgccatcg tgatcgcggt gggcttcacg
     1801 cccttgttgc tggcaccgct ggtgccctac atcacggttg gtgttttcct ggccaccatc
     1861 atggcggtat ccgccatcgt gacgctggtg atgctgccgg ccgtcttgca gcccatcctc
     1921 ggccgcaccc cgcagggcgc cgcctgaaac aggagatcga catcatgatg aaccatctgc
     1981 ttgtacgcct cgcggcaatc ggcctggcac tgccggcggt cgttcacgcc caggccttga
     2041 ccgacgtcgg cgagatcatc gaccgcgcca acctggctgc ctactacgcc ggcgacgacg
     2101 gccgcagcga ggcccgcatg gcgatcgaag atgcccaggg acggacccaa ttgcggcagt
     2161 tcacggtgtt gcgccgggat cgcgccgagg gcggcgacca ggacttcatg gtgttctttt
     2221 cgcggccgtc cgacgtgcgc ggcacggtgt tcctggtagc caagaaagtc gaccgggacg
     2281 acgaccgctg gctgtacctg ccaggcctcg acctcgtgcg gcggatcagc gccggcgaca
     2341 agcgcaccag cttcgtcggc tcgcacttct tctacgagga cgtgtcgggg cgccatccgc
     2401 aggcggacga acacacgctg gtcgagacca acgaccagca ctacgtgatc gagagcaagc
     2461 cgaagaatcc gggtacggtc gagttcgccc gctatgtcac gcgcatcgac cggggtacct
     2521 tcctgccgat gaagaccgat tactacgacg cgcgtgatcg cgtctaccgg gtcatggagg
     2581 tgcttggcgt gcaggacatc ggcggccatc ccaccgtcac ccgatcgcag atcagcgatc
     2641 cggtgaacgg cggccgcacg ctgatggagt tccgctacac ggaatacgac gtcggtttgc
     2701 cggctgacat cttctccgag cgttacctgc ggacgccgcc ggtcgactgg ctgcggcgcg
     2761 gcggggagta gcgtgatgcg tgccggcgtc ctcctggtgc tcctggcggg cctgctgatg
     2821 gcgacggaca cgagcgcgtg ggcgcaggac gacccgtggg gcgggaccgg cgacgacccc
     2881 ttcgcgcagt tcgaggagga cgatgactgg gaagatccct gggcgaccgg ccgcggcggg
     2941 ctgagctggc acggattcgt cgagggcggc ctggggggcc ggtggcagtc cgtcgaaaac
     3001 ctcgaccggc tgacgctggg tgaactgcgg ttgcgggccg agacgagcta cgatcacggc
     3061 agcttccgca tcgacttcaa gggcgacctg gcctgggacc aggtcgtgga ggacctcgac
     3121 ggacagctgc gcgaactggc cgtgtcgttc cgccccggta ccagcatgga tgccaagata
     3181 ggccgccagg tattgacctg gggcaccggc gacctgttgt tcctcaacga cctcttcccg
     3241 aaggatttcg tatccttttt tgccggccgc gacgaggaat acctgaagtc gccctccgat
     3301 accgtgcgcc tcagcttgtt cagcccggta gtgaacgtgg acctggccta tacgccgcga
     3361 tttacaccgg atgagtatct caccggcgaa cgcttcacgt tctggaatcc cgcgctcggc
     3421 gaggccgcgg cgccggcccg gccgatcaag gccgatgagc cgagcagcgg ggagtttgca
     3481 ctgcgcatgt accgcacggt cggcgcaacc gaatatgccc tgtacggcta cagcggccgc
     3541 tggaaacagc cggtcggcct ggatgcgagc ggccagctgc gcttccccag gctgcgcagc
     3601 tggggcggca gcgtgcgaac gccgttccac cgggggctgt tgaacgtcga gacggcttat
     3661 tacgactcgc gcgacgaccg cgacggtgac gaccccgcca ccccgaactc cgaactgcgg
     3721 ctgttgctgg gttacgagca ggaactggtg cgaaacttca ccgctggcac gcagctgtac
     3781 gcggaacgac tcatgcacca cgaccgcctg gtggccaact cgccggatcc ggggcgcgag
     3841 cgcgagcagt ggcgcactgt cgccacgctg cggctcagcc atcgcgccat gcaggacaag
     3901 ctcacgagct cgctgttcat gttcgtttcg ccgagcgacg aggacttcta cctgcgaccg
     3961 tccatcgcct ggcgtcgcga cgaccactgg ctgctcgcgg cgggcttcaa cctgttcggc
     4021 ggcagcgaac aacacacttt cttcggccag ctggaagaca acagcaacgg ctggctgcgt
     4081 gttcgttatc actactgatc ggatgaggag aaatcaatga ccgtcgagag atccgttacc
     4141 gccttcgcgg gcttcatggt gctgctcagc ctcgcgttgt ggtggctagt ccatccaggc
     4201 ttcatctggc tgacggtgtt cgtgggcttc aacctgttgc agtcgtcgtt caccggcttc
     4261 tgcccggcag ccatcgtatt caggaagctc ggcatcaaga ccgaagcaga agtggcgcgc
     4321 ggcgcgaccg gcggccgggg cgtcggcgcc gcggcctgaa tacgcgatgc agggatcgca
     4381 gcgggggatg atcgaggccc tgtccgagcc gggggcgtac ccgcccccgg tggacaaggt
     4441 cgaatacgtc gagacccata tttcctgggt cttcctggcg ggcgactacg cttacaagtt
     4501 caagaagccg ctcgatctcg gtttcctgga tttcacgacc cgcaagcgtc ggctgcgctt
     4561 ctgtcgcgag gaactgcgcc tcaacgggcg gctcgcaccg gagatctacc tcgatgtcgt
     4621 cggcgcgcaa cccacgcgag acggctggcg cgtcggcgaa ctgactgcag acgccgagcc
     4681 ggcggtccgc atgcggcgct ttccggcgga ggcgcggctc gaccgcgttc tggaggccgg
     4741 ccggctcgac gccgaagtcc tggagaactt cgcgggtgag ctggcgcatt tccagcgcag
     4801 cctgccgccg gcgcgacccg gcgacggctt cggcgacgcc gccacagtca cgcggccggc
     4861 gctggagaat ttccaggcac ttccggtcga gccgctcgat ggcccgggcc gccagcgcct
     4921 cgatgagctg aggcagtgga cccaggctcg tgcagcggtg ttggcggaac gcctcacggc
     4981 ccgcctggca agcggcttca tccgcgaggg gcacggcgac ctgcatctcg gcaacctggt
     5041 catgctggac gggcgcgtgg cggctttcga cggcatcgag ttcgacccgg cgctgcgctg
     5101 gatcgacctg cagagcgagg tcgccttcct gctcatggac ctcgagtcgc gcggtcatgg
     5161 cgcgctcggg tggcgtttct acaatgcctg gctggcggcg ctcggcgact acgacggcat
     5221 cggcttgctc aactggtacc tggtgtaccg ccacatggtg cgcgccaaga tcgacggcat
     5281 ccggctgtca cagggcgagc ttgcgtcgag cgaagcgaca cgcctcgcgg cacgtcaggc
     5341 ggcgcatatc gagctggcca cccgtcatgc acgccccccg acaccgtcgc tggtagtcat
     5401 gcacggcctg tccggcgcgg gcaagagcca cctcgcgcgc cgactggcgc cccgcctgcc
     5461 cgcggcctgg gcgcgctcgg acctcgagcg caagcgactt cacgggctgg atcccgggtc
     5521 cgcagccagc gccgccgtcg gcgacggtct gtactcggcg ggcgcgactg agcgcaccta
     5581 tgcacggctg gcggagatcg cgcgcggcgc gctgcgcagc ggcgtcagca tggtcgtgga
     5641 tgccgcgttc ctcgaatccc ggcggcgtga gcagttcatt gatctggcgc tggaatgcgg
     5701 cggccatccg gtcgtcgtgg cctgcgaagc gcccgcggaa ctgttgcgca agcgggtggc
     5761 tgcacgccgc aacgatccct cggacgccga tatcgcggtg ctggaatcgc agcttgcacg
     5821 tccgatcgaa atcggaccac gtgaggcgcc gtatcgcgtc gcggtggaca ccagcgggga
     5881 acccgacatc gacgcactgc tgaaccggct gcggtcgacc agggcctagc ggcgccgcag
     5941 tctctcgacc tcagcggtgc ccgtcgccag gtcagcccgc aaaaaatcgc tgttccctac
     6001 atccgtgtgg caacgtggaa tccggcacgg tgccacgcga tggccgtcaa gccgaaatag
     6061 ccgtgcatca atagcgcgaa gttccacgcg atgctgttgt cgatctgcag ctcggggaag
     6121 tagttggcca gcggaatgtt ggccaggccc agcagcagca tggttgcgcc aaacgccttc
     6181 ttggcgtgga tcacgccatg tcccacgcgg acctgcccct ctccgggcag ccggttgagg
     6241 cgcgccgcca gcaccagctg cgccagtgcg gtgaacgaga agtacaccgt cactccgtag
     6301 cgccgcatca gctcgtaggc gcggccgtcg gtgccgagaa acaccacgta aagcaccatg
     6361 aacagcgcgc cgaccagtcc cagcacggtg atccagtcgc gcgcccgccc ggtctcgccg
     6421 aggtgcccga gccaggcacg caccagcagc cagtagccgc cgatcaacgt cgcggtcggc
     6481 agcatcatgg cgcgaaaaat gtgtatcgag gggtcccggc gggcagcacc gctgatggag
     6541 gtgcagccct cgaggaacgg gaagcaggtc ggcacctggc cctgcgaggc ggacaccgtg
     6601 tacgtgatca gcaccgccgc ggtcggcagc agccagcagc ccaacgcaat cggcgcggcc
     6661 cgcatcagtg ctccgcgtag cgccggcccg ccgcggtgag catccacgcc gtgccctgca
     6721 acgccggatc gcaggcactc tccgacgggc aaccggcgca ggccgtgccg cactcgcccg
     6781 cgcgttgcag cagtccgcgc cgctccagtt cggccaccat cgcctccacc atcgcgggag
     6841 aaacgtcgat cgctgccgcc agctcggcgg tgttgccgac ctgcccggat gccacggcgc
     6901 tcaacaggcg tttaaacaac cgttcctcca aggcccgcca tgactgcggc ctggtacacc
     6961 agcgtaccga acagcagcgc gctggcaagc atcaggcccg tgctggccgc ggtccagccc
     7021 cagctgcgcg tttcctggcg taccgcggcg acggtggcga cgcaggggat gaacagcatg
     7081 ttgaccacca ggaaagcgag cgccgaggcg ggggtcacga gggcggccag cgtggtgccg
     7141 agagcctggc cctcggtgcc gaagatgatg cccagcgcgg cgatcgagtt ctccttggcg
     7201 acgaaggcgg acagtgtcgc cacgatcagg cgccagtcga ggcccatcag cgcaccgacc
     7261 ggttccagcg cccgaccaaa cgccgccagg taactgtcgt tgacgtgtgc gcccggatag
     7321 ctcaccagca gccagatgat catcgaggcc agcacgatga tggtggccgc ccgcttcaca
     7381 aaggtccaca cgttggtggc ggcaaatacc aggatggtgc gcggattcgg cacgtggtag
     7441 agcggcatct ccatgacgaa ggccagccgc tcgcgccgga atgcgaagcg gtccagcagc
     7501 aggccgatca acaccagcac cgcaaggttg cctaccacca ggccccacgc taccagggcc
     7561 ggatacaccg ggaaaaacag cggtaccagg aaggccagca ccagcaggcg cgcgctgcag
     7621 ggaacgaagg gcgtgagcat gatggtgagc agtcgtgcgg accgtgactc gatcacccgg
     7681 ctgcccatca ccgccggcac gttgcagccg aagcccagga acagcggcag gaagctcttg
     7741 ccatgcaggc cgatgcggtg catgaagcgg tccatgacgt acgccatccg gctctggtag
     7801 ccggtgtcct cgagaaagcc gagcgcgatg aagaacacga acagcaccgg cacgaaggtc
     7861 aacaccacgc cggcgccggc gagcatgccg tccaagagga ttccggacag ccaggccggc
     7921 gccccggcga gtacgccggc gagcgcagtc gagatcctgg cgacgaccgc ttcctcgagc
     7981 cactcctgca ccgggccggc gaccgagaag gtcagccaga acaccaggcc gaagaccgac
     8041 agcagcagcg caagacccca gccgggatgc gtcgcaatac ggtcgaatcg atcggtgaag
     8101 cttacctggc cgagtcgcgg ccggcgcacc gcggcgcgca ccatgcgacc gatccactcg
     8161 tagcgagccc cggccacatc gagcaccgcg tcttcgtgct cacgcagcaa ctcgtgcacc
     8221 tcgtgccagg tctccgccgg cagccactgc tgcgccgtca cggtgatctc gcgatcaccc
     8281 tcgagcagct tgagggccgc ccagtccggc ggcatgcctg cgggtgccga gggagccagg
     8341 agtgcaagga ccttctggaa tacttcgcgg tgggccgccg cgatctcggg gcgatccgga
     8401 gcgaaagcct gcttgccgtc tgccacggcc gccgccgccg cgagcacctc ggccacgccc
     8461 tcgttgcggc tggccaccat cggcaccacc ggcaggccga gcgccgcctc gagcacatgt
     8521 gcgtcgaact cgatgccctc gcgccgcgcc acgtcgacca tgttgacgcc gagcaccacc
     8581 gggatgggca gcagcagcaa ttccgccagc agatagaggt tgcgctccag cgcagcagcg
     8641 tctgcgagca tgacgatcac gtccggccgt tcccgcagga tgaactcgcg ggcgatgcgc
     8701 tcctcctccg agttggaggt caggctgtag gtgcccggca ggtccaccac gcggaagtcc
     8761 cgcccggcga tgcgcgcgga tccttcgcgg cgttccacgg tgcggcccgg ccaattgccg
     8821 acttcggcgg tgaggccggt gaggatgctg aaaaccgtgg acttgccgac gttgggctgc
     8881 cccgccagcc cgatgaccag ccgacgcgac tcagccgggc gcgccccgct cgacggcggg
     8941 gcgagaggct gcagattggc attcatagac ggttaccagg ccggcctgga ttcggccgag
     9001 ggcgattcgt gtgtcgcgga cgcgggccag catcggtccg aacccgcggt tctgaagtac
     9061 gacgatttcg gtgccggcgg tcagccccat ggcggcgaga cgcgcagtca gttcccggcc
     9121 gccggtgacc ctcgcgacga tgccgcagaa gccgtccggc agctcgctca gcacaatcgg
     9181 ttcggcgctg gccatgaggg aatcctgtcg ttaatctggt gtgcgacgct ccacgtcgcg
     9241 acgctgccgc tgcaggttga cgccagtata tgcagcccgc cagggcgctg tagatacgca
     9301 tttaacgcaa atgattctca attaaaaagg tgcctcaagc cccgcaggaa gaattacatg
     9361 ccattgaaaa tccgaatgtt ctttgcccag tccatgatcg cgagcctgct ggcattcgtg
     9421 ggcgcagccg catacgccgc cgagccgggc ctggccgtgc tgatggagcg catgcagacc
     9481 tattcccaca agctgcagct ctccgtcgcg gcaaggaacg cgccgctggc cggcttctac
     9541 ctgcatgaac tggaggaaac cgcggagtcc atcatcgaga acatcgcgag ctatgacgag
     9601 cagcccgtcg gcgagctgac ccgccagatg ttcatgccgg cattggagtt gctcgaggat
     9661 gcggtcgatg acgccgactg gtcggccggc gaccagcgat tcacggcgat gctggaggcc
     9721 tgcaacggct gccacgtcgc gtcgggacac ggccagatcc gcatcgttcc ggcgagcggc
     9781 aaccccttcg cccaggactt cagcgtgcag cccgactgag tccgttgtcg tcaggcgccg
     9841 agggcccata gccatgcgcc cgccagcgcc agcgtcgcca gtgtgaccgg cacgcccagt
     9901 gccgcgtgcc ggcgccagga gatggtgacc ccgccggccg acgccaggcc caccacgatg
     9961 atgttggcga tgctgccgat gatcagcagg ttgccggcca ggccgctggc caggccgagg
    10021 atcagcccgg cgctttcacc ctgcgccgcc ggcagcaaca gcatcaccgc cggcacattg
    10081 gagaccaggt tcgacagcac cggcgtcgcc acgaacaggg cgagcggcgc tccaaggtcg
    10141 aggccgcgcg cggcgagcca gtcgagggcg gcggccgaga gcccggtcgc ctggaatgca
    10201 tgattgacga tgaacagtcc ggcaaacagc accagtaact gccagtcgac caggccgagc
    10261 gtctggcgcg agtgcatgcg gcggctggtc agcaggatgc cggccgcggc cagggcaagc
    10321 agttcccgcg gccacggcgc ccacaggaac agcaccagca gcgccgcggc caccacgagg
    10381 cccttgcccg tttgccagcc gtccacctcg ggcggtgtcg ccggaacgcc gggctcgccc
    10441 tgccgccacc ggccgcgcgt cagaagtgca atcacacccc aggtcagcgc caggccgccc
    10501 acgaccggcg gcaaggcata cagcgtgtac caggcgaact ccagccccag cgcctcgccg
    10561 atcaacatgt tctgcggatt gccgaccagg gtcgcggcgg accctacgtt tgccgcgcag
    10621 gcgagtccca ccaggtacgg cacgggatcg agcccgcgcc ggcggcaggc ctccagcaat
    10681 accggcgtgg ccgccaggca gacgatgtcg ttggagaaca ccgcggacag cgcgccggcg
    10741 ccgaacacca gtgcgccgag cagcagcgcc gggccggtgt cgcgactcgc cagccaccag
    10801 gtcacccgcg cgaagaagcc gctctggcgc aattgcgccg acaacaccat gaagccgaac
    10861 agcagcgcca tggtcgggcc atcgatcgcc gcgaccgccg catccagcga cacggcctcg
    10921 gccgcgacca gcacgatcgc accgagcaat gccacgccgg tgcggtcgag ctgcaggctg
    10981 gggacccggc ccagcagcat gccggcgtaa acggcgacga acacgcccgc gaccagccct
    11041 tccattgaac cccactccct ccggcggaac tgccgggcgc ctagcatagc gaagccggtg
    11101 ccgcccgcct cgatgccccg ttcaggcgtc cctgacctgt attgaccggt cggccagtta
    11161 attgtagatt ggcggccatg agcagcaacc ccacgacccg tgacccggac ctcacccgcg
    11221 aacggatcct cgcggcggcc catgcgttgt tcgtggccaa gggctttgcc gcggtcagca
    11281 tgcgcgagat cgcgaaccgc tcgggcgtga ccaagagcct catccaccat cacttcggca
    11341 gcaaggaagc gctctgggaa ctggtcaagg aacgcgccat tgcggcctac gccgagaacc
    11401 aggcagccga gctgcgcgag gcctccgagc cggacgcaga tttgctgcgc cgcggcatcg
    11461 ccaactactt ccggtttctg cgcgacaacc ccggtgtggt acgcctgttt gcctggaccc
    11521 acctggacaa cgacccgagc tgcggccggc tggatgcgga actcgtgcaa ctcggcgccg
    11581 agcgcatccg ccaggcgcag gcccgcggac tgctgcgccc ggacgtgaac ccggcccacg
    11641 tggtgaccat gttcgtcaac gcctgtacgc aatggttctc cgcccgcgcc caccattcgc
    11701 aatggcccgg tgtcggcggc gaccaggaat acctcgacga cttcctcaag attttcatgg
    11761 aggggctgtt gcctcgcccc tgacaggtca cgtcatgcat cgtaacaagc tcatcgctct
    11821 cgcggccctc ggcgcaatgt cgctcgccgc ctgcgacgcg ccgcgcggtc ccgccccgcc
    11881 gccgacggac gccgagcaca tcacgccggt gcgcgtcgcc gaggtgggaa tttcggcgcc
    11941 tgcgcnnnnn nnnnnggccg aagtcggcct gtcggccccg gtgcgcgaac tgcgcctgcc
    12001 gggcgtcctg cgtgccgcgc agcgtgccga gccggcgttc ctgcaccccg gccacctggc
    12061 cgagcgtttc gtggcgcgcg gcgatcgcgt cgtcgccggc cagcggctcg cgagcctgca
    12121 aaatccatcg ctggggccgg cggtggcgtc cgccgaggcg cgcgtgcgcg agctcgacga
    12181 gcgcctgata cagctggacg ccgatcacgc gcgcgcgcag gagctgcatg cccgcggcct
    12241 ggcctccgag gagcttctcg atcgcacgct ggccgagcgc aatgccaccc gccaggcgcg
    12301 cgcccaggcg ctcgccgccg tcgcccaggc acgcgaccag ctcgccgaag ccgtgctgcg
    12361 cgcgccgttc gaagccacgg tcggcgacct gctggtggag cccggcgatt acgtgcagcc
    12421 cggccagccg gtgctcgtgc tcgccggcga ggacggcctg gagctggagg tgcagatgcc
    12481 cgagggactt gcgcgcagcc tggcgcccgg gacggtggtg gagatacgcg ctgtcggcac
    12541 gggccgccgc atccagggac gcgttcgcga ggtcggcgtg gcgcgtggcg gacgtcccgc
    12601 cccggccgtg attgccctgc ccgcgaccga aggctgggag cccggcatct cggtgcatgc
    12661 cgcaatcacg ctcgccgagg acccgggcct gacggtcccg ctgggcgcgg tggtcgaccc
    12721 cggcaccggc caaaccaggc tgttccggat cgtgaacgac cgcgccgaat tggtgccggt
    12781 ccaggtgggc cgcctggtcg gtgagcgcgt catcgtgcgg ggcgaactcg ccgccggcga
    12841 gcgggtggtg attgcggggc accagcagct gctcgacggc gaggcggtga gggtcctgcc
    12901 ctgaacgcca ttctcgccag cctggaacgg cggcggctca tcctggccgc cgtggcgacc
    12961 ctgtcgctga tcggcctgtt cgcctggctg gagatgaacc gccaggagga cccgttcttc
    13021 ccctatcgct acggccaggt gctggtgagc tggccgggcg ccgacgcggc ccaggtcgag
    13081 cgactcatcc tcaacccgct ggaggaggaa atcggccaga tcgaggaagt caacgaattg
    13141 cgcggcacga cgcgcctcgg ctttgcccag ctgatcatcg gcatgcacca gcacgtctac
    13201 gacaccgacg cggtttggga gcgcatccgt gtcgccgtgg cgcgtgcgga gcgcaagttt
    13261 cccgacggca ccggccaacc ggaagtactg gaccgcatga tggacagcga gggcatcgtg
    13321 ctggccgtca ccggctcgga cgacctgctg gtgctcaatg atgccgcccg cgcactgcgc
    13381 cgcgacctgt tccgcatgga cgacatcgca cgcgtggagc tgttggccga cccggtggaa
    13441 cagctggtca tcgggctcga tccctcggtg ggggcgaacc tcgagatcga cgccgccagc
    13501 atcgccgcgc agctcgcggc gcgcaacgtc accatgccgg ggggatcgat gacgcgcaat
    13561 ggacgctcgc tggcgttgcg gccgcaaacc gagttccgcg acctcgaaga actcggcgaa
    13621 acaccgatcc gcctgccctc cggcgcactg ttgccgttgc gggaaatcgc cagcctgcgg
    13681 ctcgcgcccg ccgacccgcc gaccgagcgc atgtggttca acggtcgggc ggcggtcggc
    13741 ctggggatcg tgattccgga gaaccgcctg aactcggtgc gcttcggggc gcaactgcgg
    13801 gcgctggtgg acgagttgcg gcccgactac gcgccgctcg agatccacga aatgttctac
    13861 cagccgcagt gggtcgagga ccggcttgcc gaactggctc gctcgctgct gacgggcgtt
    13921 gcggtagtca tgctggtgct gctggcgttc atgggactgc gcctggggct gactgttgcc
    13981 gccgtgttgc cgctggtcac gctctcatcc ctggcgatct acgcgatggg cggcggggtc
    14041 ctgcaccaga tggccgtcgc cgggctggtc atcgccctcg gcatgctggt agacaacgcc
    14101 atcgtgatgg tggagaacat ccagtggcac ctggaccagg ggcgcgcgcc gcgcgaagcc
    14161 gcggtgctgt cggtacgcga gctggccggg ccgctggcgg cggccaccgg caccacgctg
    14221 gccgcattca cgccgctgct gctcgcctcc ggggacaccg gcgacttcac gcggggcatt
    14281 cccattctcg tcatgctgac gctcgcggtg agctacgtgt acgcggtatt cgccacgccg
    14341 gtgatggcga ccctggcgct gcgcccgaag gcgacggccg ccgccgatcg caccgcacgg
    14401 cttggcgagc gcctcggatc catcgccgtg gcgcggccgt ggtcagtggt ggccgtcgcc
    14461 gcggcgctgg tggcggtcgg ggtattgctc tctggcctgc tgccgcgcga tttcttcccg
    14521 agcaccgacc gcaaccagct ggtcgtggac ctgcgcttct cggaaggcac ggagctgcag
    14581 cacaccgccc tgcaaaccaa tgcgatcgcg ggtgagatag ccgggctgcc caccgtcacc
    14641 gacgtgcacg tgttcgccgg cttcagcggc ccgcgcttct attacaacct gatcaagatt
    14701 ccgcgcacgc cgcacctggg gcgcatcgtg gtcgtcgcag agcgggatcg cgacctgccg
    14761 gcgctgatgc actgggtgcg ccagcgtgcc ggcgagatcc tgccggacgc tcacgtagtc
    14821 gccgcccgcc tgggccaggg cccgccgctg gatgcgccgc ttgagatccg ggtgttctcg
    14881 gaagatcccg cgcaactcgt cgcggtggct gagcaggtca tgaagctgct gcgcgacacc
    14941 cccggcgcgg tggacgtgcg gcacagcctc ggcaccggcc tcgcgacctt gtcgctggac
    15001 atcgacgacg cggcggcggc gcgactcggg ctggcgcgct ccgacgtggc cacgctgctg
    15061 tcggccgtaa gcctcggcaa ggaattctcc acctggcgcg cgcgacgcga accgctgccc
    15121 atgttgctgc gatctcccga gggccgcgcc ttcccggtcg aggcactcga gggactgccg
    15181 ctggccctgc ccggcggcgg cagcgtgccg ctcggccacg tggtgaacat ccgcgccgag
    15241 tggcaaccgg cggtcatccg gcactgggac atgcagcgca tgacccaggt gctcgcagaa
    15301 accgcggacg gttacaccta cgaccaggtg ctgtcggccc tcgcgccacg catcgacaag
    15361 ctggaactcc cgcccggtgt gcggctggtc tacggcggcg ccgcggcaga agcgggcgag
    15421 gccaactctg cgttgttcca gatgctgccc atcggcgtcc tgctgctgct cgtgttcctg
    15481 ctctaccagt tcaattcctt ccgcctgctc ggcatcgtgc tggtcaccgt gccgctggcc
    15541 gtggtgggcg tcacacccgg cctgttgctg gcgcaacagc ccttcagctt caccgccatt
    15601 ctcggcgtgg tcgcgctggt gggtattgtc gtcaataacg ccatcgtgct gatcgacctg
    15661 atccggcggc tggaaacgga cggcatggac gccgcagcgg cgatcgtggc ggcggtcgcg
    15721 cggcgtaccc ggccgatcct gatgacgacc gcgaccacgg tggccggcct gttgccgctg
    15781 acactgaccc gctccaccct ttggccgccg ctggcatggg cgatcatctc cgggttggtc
    15841 gcctcaacgc tgctgacgct ggtcgtgatc ccggcgctct acaagctctt gatgacagcc
    15901 aaggagcggc cgacagcgcc ggctcaggcg ccgggcagcg tgtagccctt cgcgcggaac
    15961 aacgccaggc aggctgcggc gacgcgcggg tcatagcggg tcccggcccc cgcctcgatc
    16021 tcggccagcg cgctttcgat cggccggcgc ggacgatgcg gacgcgggct ggacatcgcc
    16081 tcgacgacgt cggctacgcc aatgatgcgg gcctcgagca ggatctcctc gccccggcgg
    16141 ccgtcggggt aaccgctgcc gtcgtagcgc tcgtggtgtt gcaggacgat gtcggcgagc
    16201 ggccaggaaa aatggctccg gcgcaggatt tcgtaaccct gccgactgtg gctcttgacg
    16261 acttccattt ccatcggggt caggcggcct ggcttggtca gcacctcgtg cggcaccgcg
    16321 agcttgccga catcgtgcac ctgggccgcc agcccgatgc ggtcccgggt ctcctcgtcc
    16381 aggccaaggt ccacggcgat cgcgcacgcg aggtgcgcca cccgccgttc gtgtcctgca
    16441 gtgtagggat cgcgcatctc gacgatggtg ttcaccacgt cgatggtgcc gcgcatgttc
    16501 gcctcgagga attccctgtg gcgggcttcg cgctgctcgt gcgccttgcg ccgcaacagc
    16561 ccccaggcgc tctcggccag caggcggacc cggtcaacgt cctcgtcggt gtacgggctg
    16621 cgcttgttgc cgacgccgag caccattacc gggctgccct tctcgagcac cggcaccgcg
    16681 aggtgcctgg ccagcggcga atgcccctcg ggcaggccgc gccgatagtg ctcccgggca
    16741 aggtcgttgt gtacgaccgg gcgcccttcg cgcacgcaat ccgcccagat gccggcacga
    16801 tcgagggaat agtgcgactc cgactcgatg gcgcaagtca gcagggtccg cgccgaccag
    16861 caggcgaggc ggatgtcgac gcccttctcg acgacgtgca ggtaggccga gcgactctcg
    16921 gtcagctgct cggccagctc cacggcccgc tggccgacac cctgctcgtc aagcgtcgcc
    16981 gcttcctcgt gcagggacag caacgcgctg accagtgcgt agcgctccgt agcttgatcg
    17041 tattgctcca gcattcgccg gggccctccg tcaggcggca tcgagccgca cgatgaacac
    17101 gctgccgcgt cccggctcac tgtcgaccga gatactgccg cccatggcct cgacgaggcg
    17161 ccgcgagatc gccagcccga gcccggcgcc cggccgcccc gcttcgccgc ctccgagctg
    17221 cgcaaatggc acgaacaggc gctccagctc tgccgcggga atgccccgcc cggtgtcctc
    17281 gacggcaatt tccacctggc cgtcggtgcg ccggcagcgg acgatcacct cgccgtgatc
    17341 agtgtacttg actgcgttgc tgaccaggtt gttgacgacc tgctcgagcc ggcgccgctc
    17401 cgccagcgcc gcgcaatatt cgatctcggt gtgaatggcc aggcccttgg cgatggcctg
    17461 ggggcgcatg gtgtccgccg cccgcagcaa catgtcgcgc agcggcgtcg gctcgcaggg
    17521 caagcgggta tccgaggcct gcaaccggga gatgtcgagg atatcctcga tcagcgccag
    17581 cagccgctca cccgcacgcc gcacgatcgt gagctgcttt tcctgctcat cgttgaccgc
    17641 gccgggcgcg cccgacaaca gcaggctgct gaagccgatg atcgagttga gcggcgtgcg
    17701 cagctcgtgc gagatattcg caaggaactc ggtcttgatc cggtcggctt cctcggcacg
    17761 atcacgtgcg gcggccagct cggcagtgcg ctccgcgaca tgcttctcga gctcgtcgcg
    17821 cgcgattcgc aaggcctccg cggcttgatg ccgcgcggtg atgtcctcgg caaccaccat
    17881 gacgccgaag tcgccgtcgg ccatggtaat gcgtgaggcc gagacgctca cgtgcccgac
    17941 cgagccatcg cgtcgccggc accgggcctc cagcccgaga agttcgtcgc cgccgagcac
    18001 ctggcgctgc atcgcgtcga attccgcgtg gtgcgcgtca tcctccagca cgatcggcag
    18061 gcggcggcca aggacctcgt cccggcgcca gccgaaaacc cgctcggcgg cgggattcca
    18121 gacgtcgagc acccgaccct cggcatccag tccgaacaca ggcaccggcg ccgcgtcgat
    18181 caacgcggtc atgcgccgcg ccacctcgcg gaagcgggcc tccgcacgct cgcgcgcgcc
    18241 gcgctccgtc gccctgtcca tggccagcgt gaggtcgcgc gccatgtccg acaacaggtc
    18301 cagagtgtcc ccttcgaagt agcccgtctc gcgggagaac actgcgaagc gggcctcggg
    18361 gatgggcgcg ctgccgagcg gaaagctggc gaaggcccgc acgccgcgcc gcaggcaggc
    18421 gagccgcgtg ccggcgccca ccggcgccgc ttccacgtcg ttcaccacga tgatgccagg
    18481 ctgagctccg tcgccgcgag gaaaggcgaa aacatcaccg ggggccggcg ccgacaacag
    18541 ctgcgacgcg aacgcctcga cctccgggcc ggccgaagcc gccaggcacg gaccggtctc
    18601 gccggggcgc gccactgccg ccagcggcag ggacgcgtgg gtgacggcga tgcggcaggc
    18661 ctccgccagg agcgcttcga gttccggcag gcgggcgatg gcccggttgg tctccgagag
    18721 cgtccggtag agcttcgtgc ggcgcttgag ctgcgcctgc aggcgtttgc gcggcccgat
    18781 gtcctcgatg acgcccacca ggtattccac ctggccattc tcctggcgaa cggcggaggc
    18841 cgtcagctgc gcccagaaga tggagccatc cttgcgccgg tagcgctttt ctatggagaa
    18901 gtcctcccgc tggcccgcca tcaccgcctt gcccagcgag agctccctgg gaagatcctc
    18961 cggttgcgag atctgccaca cggtcatgcc ctcgagctcc tcggaggaat acccgagcag
    19021 ttccgcaaag cgcgggttga ccagcaggaa gcgcccgtcg gtcgagacgt gcgcgaggcc
    19081 gaccgcggtg cgatcgaagg tgttcctgaa gcgcgcctcg ctgcggcgca ccgcctcctc
    19141 ggcccgcgtc cgctcggtga tgtcgcgcag gatgccctgg atgaaagtct cgccgtcgac
    19201 ttcgaaggac cgcacactgg cctcgacggg gaactcgctg ccgtccttgc ggcgatgcca
    19261 ggtttcatac accaggccgc cttcctgccg cgcgatcccc agctgcctgc ggcccgccgc
    19321 caccagcgcc ggcggccgca gctccagcgt atctcgtccg atgaactcgt cacgcgaata
    19381 cccgaacgcg gccagcgccc ggtcgttaca gttcacgatc cggccgtcgg cgtccatcaa
    19441 caggacgatg tcgttggcga agcgggtaag gtacgagaaa tgctggttga gcacgtcgcg
    19501 ctcgcgcttg acgctctcga gcattgcgcg attgagcagt tgctgctgcc ggaacagcac
    19561 caggatcagc cagccggcga tgagcacggc caccagcacc accagcgcgg tgttgcgcag
    19621 cagcttgaag acgccggcat agacgtcgtc ccggtcgatc gcgccgacca cgatccagtg
    19681 gacgcccgac acccgtgcca tggtgagcag gacggaccgt ccgtcgggca gagagccctc
    19741 gatgatgccg tgttcgcccc gcggcgcgta gtccgatccc ggctcctgcg tccgggtcgg
    19801 gaacaccagc agcctgccgc cgtgcacggg cgcttcgaaa cccggcagga acaccagctc
    19861 cccgtccggg cccaggctcg ccagcgacgt ttcgccgctg atgcccgcca gcggccagcg
    19921 ctgcaggatg gaaacaagcc ggggtcccgg gtcgatggtc gccaccagcc aactcggccc
    19981 gtcggttgcg ccgttgcggc tcgccagccg ataggtcagc agcgcgctca tcgtcccgcc
    20041 gtccgcagga gcaacccccg agaacccgat ctggccgtcg gcggcgtcgc gaatggcgcg
    20101 tgccagcaga tcagcatcgg gcgtcccgcc accggccggc aacagccgcc cgccagcatc
    20161 cgcgagtgcg acgtcatcga gacccagcag gcgtgcggtc ggtgcgatgc gcgccagcaa
    20221 ctccgcatgg gcagccggcg attcctcgaa ggtcccgaga ccggctgcgc cctcggcata
    20281 tatgtggccc agcgccgcga cggcgccgac ctggtcgccg aaccattcgc cgacacgctg
    20341 tgccttgatg aaggcggtgg agcggagcgc agagtaactg cgttcctcga tgctctgtaa
    20401 gtgctggcgc tggacctggt agccgaccag caggatcagg ccggccaggc agaagaacac
    20461 cacactgagc aatccgcccc gcgcctgcgg cagttcgccc tcctccggtt cccgcggggt
    20521 gacccgcatg agcacgtaca gcaccgcgct cgtgacatag acgaacagca gccccttggc
    20581 ggtgccgagc cacatcaggt cgtcgacgtc gacccccagg tcgggcaaca agcgatccga
    20641 tgcgatgatc cacagcgagc ccagcaacgc gtacaccagg gcgatgccga gcgcgatgcg
    20701 gcgcggcacc gtgatgctgc gtggcccccc gggcggggtt ccgtcgctgt ccacgatcgc
    20761 tgaactccct gctgatggcg ccgggcggac agtctccggc cccgactcgg cggctattgt
    20821 gccgggaaaa actgccggcg tgttggactc cggctgcacc gacccgcaac ggctgcacgc
    20881 aagggaaggc agtgaatggt ggctacgggt gggctcgaac caccgacctc agcattatga
    20941 gtgccgcgct ctgaccaact gagctacgta gccgccgacg ggcgcctggc ggcgcatgtc
    21001 ccggaagggc cgagaattct ccccgcgccc cgcgaggctg tcaaggcgag ggccggaccg
    21061 aattgacaaa tcgcaaggcg tcccgccgcg ggctttccca gaatctgctc ccaggggccg
    21121 gatcaaccgg cccgggctaa cctgggaggt agcggccatg tatgacacca agcgactctg
    21181 gtggaccctc gggatcatcc tggtggtgtc gttcggcatc ctcggcttca tgggcggtga
    21241 aatttaccgc accatgccgc cggtaccgga cgcctacgtc agcgcggacg gcgaaatcct
    21301 ttatacgcgc gacgacatcg aacgcggccg gcaggtgtgg cagaccacgg gcggacacca
    21361 gcagggctcc atctggggcc acggcgccta cgtcgcaccc gactggagcg ccgactggct
    21421 gcaccgtgaa gccgtcgccc tgctggacgt ctacgcgcaa caggacttcg ggcggggatt
    21481 cgacgagctg gacgaaggcc cgcaggcagc actccggcaa cgcctgaaga gcgaaatgcg
    21541 caacaacacc ttcgacccgg cgagcaatcg cgtgacggtg tccgccgaac gcgcccgcgc
    21601 cattcgcgaa gtcgcggcac attacgaggg cctgttcggc gacgatcccg agctcgccca
    21661 gctgcgcgaa gcctacgcca tgatgcagtt cccggtgccc gacgcagagc gccgccagct
    21721 gctcggcgct ttcgtattct ggtcctcctg ggccgcggcc accaaccgtc cgggcgacga
    21781 catcacctac accaacaact ggccgcacga gcaactgatc gacaacacgc ccaccggggc
    21841 gtccttcatg tggacgttcg tcagcatcct gatcctgctg gccggggtcg gcgcgctggc
    21901 ctggcactac gcgatggtgc acggccacga gccgccgccg gaagtgcccg ccatcgaccc
    21961 cctgcataac ctcaagccga ccccctccat gcgggccacc gtgaagtact tctgggtagt
    22021 gacggccctg ttcggcctgc aagtggtgct cggcggcatc accgcgcatt acgcggtgga
    22081 gggccagggc ttctacggcg tcaacctgtc cgaggtgctg ccctacgtca tcacccggac
    22141 ctggcacacc cagctggcgg tgctctggat cgccacggca tggctcgcca ccggcctgta
    22201 catcgcgccg gccatctccg gccacgagcc gaagttccag cgcatcggcg tcaacttcct
    22261 gttcgtctgc ctgctcgtca tcgtgctggg atccatggcc ggccagtggc tgggcgtcat
    22321 gcagaagctc ggcctggaca tcaacttctg gttcggccac cagggctggg agtacgtcga
    22381 cctgggccgc ttctggcaga tcttcctgtt catcggcctg atgctctggc tgactctcgt
    22441 gggccgcgcc ctgtggcctg ccctcaagac gaaggacgaa ggccggccgc tggtgatcct
    22501 gctgttcctg tccaccgtgg ccatcggcct gttctatggc gccggcctga tgtggggcaa
    22561 gcacacgcac ctggcgatcg tcgagtactg gcgctggtgg gtggtgcacc tctgggtcga
    22621 gggcttcttc gaggtcttcg cgacggcggt gatcgccttc ctgttcacca agctcggcct
    22681 ggtgcgcacg accagtgcgg tgaccgccgt gctgttcgcc accatcgtgt tcctgacggg
    22741 cggcgtgctc ggcactttcc atcacctgta cttcaccggc acgccgacgg cggtgctggc
    22801 gatcggcgca tccttctcgg cgctcgaagt cgtcccgctg gtcctgatag gcttcgaggc
    22861 ctacgaaaac tggaaggcct ccaagtaccg tggctgggtg gcgacctaca agtggccgat
    22921 catgttcttc atcagcgtgg cgttctggaa cctggtgggc gccggcctgt tcggcttcct
    22981 catcaatccg ccgctggcgc tgtactacat gcaggggctc aacaccacgc cgctgcacgg
    23041 ccacactgcc ctgttcggcg tctacggcat gctcggcatc ggcctgatgc tgttctgcct
    23101 gcgcggcctc acctatcggc aggcctggaa cgagaagctg ctgagcgtgt cgttctggtc
    23161 gctcaacctt ggcctggcgg cgatggcact gttcagcctc ctgccgctcg ggctgatgca
    23221 ggtctgggcg agcgtcaccg agggcttctg gtacgcacgc tcggcggagt tcctgcagca
    23281 gccgctgatg cagacgctgg tctggctgcg agtgccgggc gacgtgatct tcagcgtcgg
    23341 cgtggcggcc ctggcctggt tcgtgttccg gctgttcgtc gggcctcgcg agaccgtccc
    23401 ggcccgctcc ggtacgaccg ttgcggaggc ccgggagtga gtcgccgttc gccccgtcgc
    23461 ccgcgtggcg gcgggg
//
LOCUS       DAOMPG010000007        22674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_977_length_22674_cov_4.143994, whole genome shotgun sequence.
ACCESSION   DAOMPG010000007 DAOMPG010000000
VERSION     DAOMPG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 22674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_977_length_22674_cov_4.143994"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLS34_00810"
     CDS             <1..1130
                     /locus_tag="PLS34_00810"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRP86043.1"
                     /translation="VTDAIAIHPGYGFLSENADFAERVEKSGFVFIGPRPETIRLMGD
                     KVSAIRAMKASGIPCVPGSDGALGDDPEANLKMARSIGYPVLIKAAGGGGGRGMRVVH
                     SDATLLNAITLTRGEALAAFGNETVYLEKYLERPRHVEFQVLADGKGNAVHLGERDCS
                     MQRRHQKVIEEAPAPGITPEQRDAIGRRCAEACIEIGYRGAGTFEFLYQDGEFFFIEM
                     NTRVQVEHPVTEMITGIDIVQEQLHIAAGHPLRMRQEDIEIRGHAIECRINAEDPKTF
                     MPSPGPVRQWHAPGGPGIRVDSHVYSGYQVPPFYDSMIGKLIAHGPNRQSAIARMQTA
                     LTEIVVEGIRTNIPLHQEIFSHSAFKSGGTDIHYLEKRLGL"
     gene            1188..2108
                     /gene="prmA"
                     /locus_tag="PLS34_00815"
     CDS             1188..2108
                     /gene="prmA"
                     /locus_tag="PLS34_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRP86044.1"
                     /translation="MPVDWLELSATVDRALADRAEEGLFAAGALSVTFQDAADAPVLE
                     PAPGETPLWPTLNVTGLFGGDADPLAILAAVHASVPGAEWRVATLANRAWEREWLRDF
                     RPLRFGRRLAVVPGGMAPPEGTIAVRLDPGLAFGTGTHPTTALCLEWLDGLAAPAPGE
                     RSPLRDALVVDYGCGSGILAIAALRLGAAAAIAVDLDAQALLATGTNAAANGVADRLQ
                     TGMPAELSAMLDGRKADILVANILAGPLQALLPRFAATLRAGGLLALSGILVGQEQAL
                     LAAAEPLFGMDPPAMREDWVRLSGRRNAKG"
     gene            2108..3493
                     /locus_tag="PLS34_00820"
     CDS             2108..3493
                     /locus_tag="PLS34_00820"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRP86045.1"
                     /translation="MFTRCSNCRTVFHITAAELRAADGTVVCGACGATFDALDSLSEA
                     RPEEPPHIHPEPEKAAAPPPVSESAAELDERARDEDEFLQELESLIGGEAFLEEPSPG
                     EEPTGTGFPQMEFSRAPLRDEDLATDLSTADEARGEFAAEDEFLVEEFEAEDTPEESR
                     FDQALLADEPDEPGDDAPAPGEPPLRDLDEDDDLDDPFLDPDSVFRIDEEPEDGEGAG
                     VFAWRDADRASRDTSAEGAGQPPAEAASPGSADAEAPVRDAPEHAAAAPGPASDAAGE
                     AAVEPLPEFARQPRPGRRWLRVVTALLAVLLLTGTWAHGQRGKLLRHPAGEAVLGPVY
                     RALGMESAPDWNPAEYRAVQWQAVADADRPDDLAVAVDFMNMASYAQPYPIIRIVLED
                     RFGRRVGVHDVPPSEYLRDHSRGSRMAGGGRLQTTVVVRDPGGRADGFRVDFCLELEG
                     RGLVCGPAPFR"
     gene            3537..4532
                     /gene="dusB"
                     /locus_tag="PLS34_00825"
     CDS             3537..4532
                     /gene="dusB"
                     /locus_tag="PLS34_00825"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRP86046.1"
                     /translation="MKIGNYSFVAPVVALAPMAGVTDRPFRALCRRLGASFAATEMVS
                     ADQRLWDTPKSRHRLDHAGEGDPVIVQIAGGDPEMIAAAALANAALGAQVVDINMGCP
                     AKKVCNRAAGSALLRDEGLVRAILEAAVATLAPLGIPVTLKTRTGWSPDNRNALRIAR
                     LAEQAGVAAIAIHGRTRACGYGGIAEYDTVRAIKLEVGIPVIANGDIRTPEEARAVLE
                     HTGVDGLMIGRAAQGRPWIFREMRAFIATGIRSPEPGAAEVRDIILRHLDALHRFYGE
                     ARGVRTARKHLGWYLAGRPGGEELRRRIVRVEDAGEQLAVVGEYFEEALDKELAA"
     gene            4574..4837
                     /locus_tag="PLS34_00830"
     CDS             4574..4837
                     /locus_tag="PLS34_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP86047.1"
                     /translation="MNGNGKTRPGGDGALGSMAQEALDQYFQTLNGHKPEELYDLVIG
                     EVEKPLFRAVLDYTGGNQSMAATILGINRGTLRKKLRLHGLLD"
     gene            4867..6426
                     /gene="purH"
                     /locus_tag="PLS34_00835"
     CDS             4867..6426
                     /gene="purH"
                     /locus_tag="PLS34_00835"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRP86048.1"
                     /translation="MAQIKRALISVSDKRGIAEFAHGLAELGIELVSTGGTAAALRAA
                     GLRVRDVAELTGFLEIMGGRIKTLHPGVHGGLLGRRGSDEEVMARHGILPIDLLAVNL
                     YPFRETVADPDCTIDEAVENIDIGGPAMLRAAAKNHAAVTVVVEPADYETVLTALRAA
                     GEVPYEMRLRLAGKAFAHVASYDTAVSNWLGWQAEMDAAEMPDDLVLAARKTLQLRYG
                     ENPQQRAAMYSYGHPRKGTVGAAEQLQGKELSYNNLLDADAALECVKLFDEPACVIVK
                     HANPCGVATGQDILDAYRRAYATDPVSAFGGIIAFNRPLDGGTAEAILKKQFVEVLVA
                     PSIDPGAAGVLGSKPNVRVLASGRWSRSPLGTIQLRSIGGALLVQDVDDAEVDTEQFK
                     VVTQRAPTEHEMRDLLFAWKVVRFVKSNAIVFCKDAATIGVGAGQMSRIYSTRIAAIK
                     AADAGLAVKGSVMASDAFFPFRDNVDAGAEHGVTAIIQPGGSVRDPEVIRAADEHGLA
                     MVFTGTRHFRH"
     gene            6472..7752
                     /gene="purD"
                     /locus_tag="PLS34_00840"
     CDS             6472..7752
                     /gene="purD"
                     /locus_tag="PLS34_00840"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRP86049.1"
                     /translation="MKILVVGGGGREHALAWKAATSPLADRVYVAPGNAGTAREPGCE
                     NVAIDAADIAALADFAAAQDIGLTIVGPEVPLVAGIVDEFTRRGLKCFGPTAACARLE
                     GSKAFSKAFFARHGIPTAAWADFSDADAACSYIRAQGAPIVVKADGLAAGKGVVVAAT
                     VAEAEAAARDMLSGNRFGDAGARVVVEEFLAGEEASFIAMVDGAHILPLASSQDHKAR
                     DDGDRGPNTGGMGAYSPAPVVDAALHARIMREVMEPTVAALAADGTPYTGFLYAGIMV
                     APDGAPKVLEFNCRFGDPETQPVLFRMKSDLVALCLAALDGRLDAVSCEWDRRTALGV
                     VLAAAGYPDAVRKGDPISGLDGDAGRDDVKIFHAGTRLEGDRVVTSGGRVLCAVALGD
                     GVREAQRKAYALTAQIRWDGAFFRRDIGWRAIGR"
     gene            7885..9600
                     /locus_tag="PLS34_00845"
     CDS             7885..9600
                     /locus_tag="PLS34_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HRP86050.1"
                     /translation="MLLATVCCLWLTAAGAEVRVDITGVDGEIEANIRATLDLVRHGG
                     REDLSEAAVRRLHSRAPRQIREAMRPYGYYRPRVQPSLEQRNGEWRARFVIDAGEPVL
                     LKEVDVRILGDGSGDGALLDIVAQSPLREGRRLNHQEYDRLRNRLQATAAMRGYFDAR
                     FEQRRLEVDPSEHTARAVLHMSTGPRYRFGSISVEQDIISAALLDRIVLLREGEPYDA
                     NALLRAQYRLSDTSYFAGVVVESGAPDEATLSVPIRIETTATRGQRIRLGLGYATDTR
                     LRGSIGVDWRHLNKAGHSAGTELRLSEVLTEISGRYRIPIGDPLQERLLFRGGLTRED
                     LADIESQRISVGVSHVTMRGGGWQRNLFADLLEERTRTPNEPEFRDLLIVPGIGMEKL
                     IADDVLFPRNGYRVRGEVRGSHHYLGARTDFLRIMAEANRVSSAGESWRFFLRSAAGY
                     GIVGNVDALPASQRFFAGGDQSVRGYGFNSLGPRDADGNVIGGRHLLFGAVEAERLVW
                     GRYALAAFLDAGNAFNDFDEGLEASVGVGVNVHTPIGTLRVAMARSITESRGMRFHLS
                     IMPDL"
     gene            9597..13499
                     /locus_tag="PLS34_00850"
     CDS             9597..13499
                     /locus_tag="PLS34_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRP86051.1"
                     /translation="MKRRRLIIPIAVLAMLAAVFAWLVGTTSGLRFLAARALPHLPVS
                     YSPGDVEGRLLGPLALGPLELAAPGLSGSIERIELDWRPMELWRGSLHILELQVRRPL
                     LSLAPTGDVAQAPPQGATGQFSLPLRLVADRLAVIDGELEADGETLVEGLQLELAGRA
                     SGSTLALDHVALRSNRGGVTGHARAGMAPATPWDIDLEWQAVVDEQEVVGHSRVTGRL
                     ARLHLVQEISAPLVARLEGVVEGLPDAPAWTIEASNEPLAPAAGPWPETLVGLATRLQ
                     LAGSPDDSLLTGNLQLPALVAGTISLAAQGGWQDHAAVVRRLAIVLPDDARLDAAGRF
                     VPGEEPAAEFSLTGERLRWPLDATEPALALPRLALHGTGADDRWRFTADARAVRAGLP
                     DVDLVAELQLTGTTLTIERLTADSAGGEVHARIAGLLETATDELRYQVTADGHIELPD
                     YPPVSASLNATGDVSGVNIADLDARLLGGTLTGAGRIAWEGEQQADFQLAFSGIDPSG
                     LAPDWPGRLDGRLGLRGLPDAASGMEIELGALRGELRSMPVRGEAALNLGRSGQVLRT
                     LSLSLGAASLQARGQLDDASVALEASLEAPELDALHDDAAGSFSASLELAGARDRPQV
                     ELEARGAGLRWQSGRIRELWIDATVDPTGATRSSLRAALTGFATEPGPGASLTLEADG
                     TPADHEARLDYHRRRQGQRVQLALAGGLDEARWIGQLQALTLTEAGQEAWALQAPADL
                     RTAADSVQLNNACMDGTLGRLCLDAAWARGGPWRGRSALAQLDLEPLSDWLGRGLIAR
                     GVVTGQLEVEADDDTFLGLSGGLALTGGDVRVAGEDGDTLLAWRSGALELAGDRDEAV
                     AELDVVLAGDDRIQGRLAIGWNEADPPLQGRLEARLGQLDIITELLPDLAELRGSAVA
                     EAVVAGTLGAPAITGRFEWLDGTAEIPTLGLAPRDIRVLAALAEGVLTFTATGRSGDG
                     EFEADGRFDLAAAGVEGRAALRGEDLLLADLPEARVTASPDLRLHYSGNNLTVGGEVR
                     IPTARIRGLGGPGAVTTSPDEVIVGPRARTEDEGLEVASRVRVSVGPDVQIQAAGLRG
                     RVEGSILTVTQPQALPWGRGELRVVDGTFGAFGQRLEIETGRLIYSGGPLENPGLDIR
                     AVRRIDTVTAGALVRGTLRQPEISLYSDPPMPRADTLSYLTLGKSLDELQAGEKQTVN
                     QAASSLALSGGGLIAQDLGRRLGFDDVAVTADEQTGGAAVVISKYLGGGLYVSYGLGL
                     FDTVNTLRLRYQFNRRLSVEAASGEQSAADFFYTFERD"
     gene            complement(13501..14409)
                     /locus_tag="PLS34_00855"
     CDS             complement(13501..14409)
                     /locus_tag="PLS34_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HRP86052.1"
                     /translation="MSPAPRILVVGGTGAQGGSVARHLLAEGRCRVRCLTRRLDSVAA
                     RALADAGAEVVQGDMADRDSLDAALAGCEAVFGVTNYFEHFAAEYELGARLLDAVVDS
                     AAEHLVLSTLPSASQLSSGKLSVPHFEHKARLEALVRGKEITATFVHVAFYYENFLLN
                     CQPRRQQDGSYAFGFPLGNAPLSAVAVADIGGVIAAILRAPREFQGRTVQIVGDQRPA
                     ADYADIIGHATGRTVRYQHVPRDDFAALPIPGAAALADMFELYRSHVGAKPEDLAASR
                     RLYPDIRDFAAWAQAHRDELRGAMDA"
     gene            complement(14432..14899)
                     /locus_tag="PLS34_00860"
     CDS             complement(14432..14899)
                     /locus_tag="PLS34_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRP86053.1"
                     /translation="MTKPGLAGRLALCGALLVLGAAGAPASASADEDEAVRAVALDYA
                     EGWATADAERMARALHPQALKRRVVVDILSGEPRVQQMAAAELIRATRAGDGKPADPG
                     PLNLRVAVLDRHGDMAVARVVSPLYVHYLQLVRWDGRWVVLGITWGTVAAPGE"
     gene            complement(14957..15382)
                     /locus_tag="PLS34_00865"
     CDS             complement(14957..15382)
                     /locus_tag="PLS34_00865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRP86054.1"
                     /translation="MAEFELHPRLLADCHRLGRFRLSHLLLHRNAGLPWLILVPEVSA
                     AVHELFELEAPDRRELVDEIDVVARFVKDSLGAQKINIAAIGNLVPQLHVHVVGRHPG
                     DPCWPGVVWGRLPDGQEWPPQRIIGIVGAISRLRPFERA"
     gene            complement(15419..16267)
                     /locus_tag="PLS34_00870"
     CDS             complement(15419..16267)
                     /locus_tag="PLS34_00870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86055.1"
                     /translation="MLIDCHVHLNNYDETHSRTADEAAADLFAAMDENGVDHAVVLTS
                     YKVNAHRPHVDEVIRVVGDDPRVTIVEGLRWRDPDERTDLYNMEERIREGLVKGIKLY
                     PGYDRYPINDPSLESVFRIAEKHDVPVMIHCGDTYSKQAKVRQAHPLLVDDVAVDYPD
                     VRFVICHLGNPWFQDAAEVLYKNDNVYADISGLTLGEFHYEFERYMLQRVRDMISYMG
                     DPGKQLMYGTDWPLAGMRTYRRFFDSLELSEEAKEGIAWRTAANLFKIDLDALAGQAA
                     EGDLAG"
     gene            complement(16325..17977)
                     /locus_tag="PLS34_00875"
     CDS             complement(16325..17977)
                     /locus_tag="PLS34_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86056.1"
                     /translation="MYTRRTGMLALLTVGAALQCPVLAEPEEPPAAAACGFSPEALEN
                     AGARIGKLSIHAENIFDLDDPAEDNALFRTMNRLHVRTRDGVIARQLLFGSGDAFRVQ
                     QLDESERLLRSTAYLYDADIRIAACDAQSVDLEVHTRDVWTLKPGFSLSRSGGESRVG
                     FDLQEDNFLGRGGSVRYVRRIDEERRSTEIGYADRNLGGRWISLDATVADNSDGHELG
                     IGLERPFYALDSRWALGARILDRRLEDPVYSLGDEIGRFREDVEHLDIYGGWSNGLQA
                     GWVQRWLAGVVYDRRRFANADGSIDPALIPEDRKLLYPFVEYQLIEDRFQRAANLDQI
                     YVTEDVSLGASIGLRLGLLSETLGADRRGATFGVVARRGYGDPLHVLWDFSAHASGRL
                     ESGEITNAIVGGTASWYLRQSERRLLFAAVRGDVAENLDLDNPLELGGDNGLRGYPLR
                     YQRGDARAQFTIEQRYFTDYYLWRLFRVGGAIFFDAGRVWGENPYGGENLGWLRDVGF
                     GLRLGSTRSSIGRVVHIDLAFPLDGDASIDSVQFLVQGKRSF"
     gene            18213..19541
                     /locus_tag="PLS34_00880"
     CDS             18213..19541
                     /locus_tag="PLS34_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HRP86057.1"
                     /translation="MRGIGGPALLVLYLLIALAPLALAALLGAPARNPWRELASGLAM
                     VGFAMLLLEFVLSGRFHGVSGRVGIDVTMRVHQLAALGLLLFLFAHPFLYAVPRLSPD
                     PMRAASFVQRMFTGEWYRSGVVAWCLLLALVPMAIFRDRLPFRYEIWRASHGLGAALI
                     AGLGLHHTLTVGTHGAHPVLAGFWVVMTGLAVLTLLFVYVLKPLSQLRRPYRVVANRQ
                     VADRCWELVVRPDHDQSVHFLAGQFAWLNLGHHPLSLTEHPFSISSAPAGRPDVAFTI
                     KQSGDFTNRIGEVPVGTRAWLDGPHGHFVLPDPHPDRIAFIAGGVGFAPIISMLRQLR
                     AERWRGRAALVYGNRVETQLLYREELRQMAGELNLELHLVLSEPPPGWTGPVGELDRE
                     MLDACLDPVDRAGWVYFVCGPPPMMNSVEHSLRDFGVPARQIVAERFKYD"
     gene            19600..20211
                     /locus_tag="PLS34_00885"
     CDS             19600..20211
                     /locus_tag="PLS34_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HRP86058.1"
                     /translation="MPTSLIIVDDFLDNPQQLREAALRLTYPQVQGAFPGRNSVERID
                     IGGLANQVSRLVGEHVEPMPPPQSHGKCRITLASDVGKAKVHVDGAHWSGILYLSRPE
                     DCRGGTEFFRHKATNTDRAPYDDREAAGHGFASARQMVTEILDRDSRDDSKWEMTMRV
                     PMRFNRLILLRPWLWHTAGENFGDSLQNGRLVYLMFFAMAAGR"
     gene            20393..20593
                     /locus_tag="PLS34_00890"
     CDS             20393..20593
                     /locus_tag="PLS34_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HRP86059.1"
                     /translation="MTKTLSFAVVHFFVAFSVGFALTGSVAIGGAIALIEPALNTVAY
                     FFHEKAWDRIRAPRHAVSPQPA"
     gene            20627..>22674
                     /locus_tag="PLS34_00895"
     CDS             20627..>22674
                     /locus_tag="PLS34_00895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRP86060.1"
                     /translation="MTALDNATPAAAPKELILSVGGMRCASCVGRVEQAARKAPGVTG
                     ATVNLASGRAFVTLADPAANAEAVAQAIRDAGFEAEPLLDAAEQASRDDTARFDESRA
                     LRNAFLLAALLTLPIFVLEMGAHLVPGMAGFAADAVGIRNLHLLFFVLATAVQFGPGL
                     RFYRTGWPALMRGAPDMNSLVMLGSTAAWAYSVVAVFFTGLLPAGTANVYFEASAVII
                     TLILAGRYLEARARGRAGDAIRRLVSLQPRTARIQRNGRTLEAPIERVRAGDLVLVRP
                     GERVPVDGCLVEGSSWVDESMISGEPLPVEKSVGDEVIGGTINGSGAFTFRATRVGAE
                     TVLARIVRMVEQAQAAKLPIQALVDRVTHVFVPVVIGLAILTFSAWLVLGPAPALGFA
                     LVNAVAVLIIACPCAMGLATPTSIMVGTGKAAELGLLFRKGDALQALQEADTVALDKT
                     GTLTRGEPALTVFRTAPGFDETRTLALVASVERRSEHPIAAAIVAGAHDRGIALHDAT
                     EFLAEAGLGASALVEGRRVAVGAARFMARQGIDVTTFTAAAAEFAAEGATPLYAAVNG
                     RCAALVAVADPLKESARVAIAALHRLGLRVVMLTGDDRRTAAAIAREAGIDEVHAELL
                     PDDKVKVVRRLQAGGRKVAFVGDGINDAPALAQADVGIAIGTGTDIAIDSAELVLMSG
                     "
BASE COUNT         3412 a         7821 c         7956 g         3485 t
ORIGIN      
        1 aggttaccga cgccattgcg attcatccgg gctacggctt tctttccgag aacgccgatt
       61 ttgccgagcg ggtggagaag agcggcttcg tgttcatcgg accgcgcccg gaaaccatcc
      121 gtctcatggg cgacaaggtc tcggccatcc gcgcgatgaa ggcgtccggc ataccgtgcg
      181 tgcccggctc cgacggcgcg ctcggcgacg accccgaggc caacctcaag atggcccgca
      241 gcatcggcta tccggtgctg atcaaggcgg ccggcggcgg cggcgggcgc ggcatgcgcg
      301 tggtgcactc ggatgcaacg ttgctcaacg cgatcaccct gacccggggc gaggcgctgg
      361 ccgcgttcgg caacgagacg gtctacctgg agaagtacct ggagcgcccc cgtcacgtgg
      421 agttccaggt gctcgcggac ggcaagggca atgcggtgca tctcggggag cgcgattgct
      481 ccatgcagcg gcgccaccag aaagtcatcg aggaggctcc ggcgccgggc atcacgccgg
      541 agcagcgcga cgccatcggc cggcgctgcg ccgaggcctg catcgagatc ggctatcgcg
      601 gcgcgggtac cttcgagttc ctgtaccagg acggcgagtt cttcttcatc gagatgaaca
      661 cacgcgtcca ggtcgagcac cccgtcaccg agatgatcac cggcatcgac atcgtccagg
      721 agcagctgca catcgctgcg ggccacccgc tgcgcatgcg ccaggaggac atcgagatcc
      781 gcggccatgc catcgagtgc cgtatcaatg ccgaggaccc gaagaccttc atgcccagcc
      841 ccgggccggt gcgccaatgg catgcgccgg gcgggcccgg catccgggtg gacagccacg
      901 tctattccgg ctaccaggtg ccgccctttt acgacagcat gatcggcaag ctgattgcgc
      961 acgggccgaa tcgccagtcc gccatcgccc gcatgcagac cgcgctgacc gagatcgtgg
     1021 tcgagggcat ccgcaccaac attccactgc accaggaaat tttcagccac agtgcgttca
     1081 agtcgggcgg taccgatatc cattacctcg agaagaggct ggggctctga ggtgcgcgcg
     1141 cggctgccgg ccgcgccgct gcgcggaccc ggggagcccg ccgggccgtg ccggtagact
     1201 ggcttgaact gagcgccacg gtggaccgcg cgctggccga ccgcgccgag gaaggcctgt
     1261 tcgcggccgg cgcgctgtcg gtcaccttcc aggacgcggc cgacgcgccg gtgctcgagc
     1321 ctgcgccggg cgagacgccc ttgtggccga cgctgaacgt gaccggcctg ttcgggggcg
     1381 atgccgatcc gctggcaatc ctggccgcgg tgcatgccag cgtacccggc gccgagtggc
     1441 gcgtcgcgac gctggcgaac cgtgcctggg agcgtgaatg gttgcgcgat ttcaggccgc
     1501 tgcgcttcgg ccgccgcctg gcggtagtac ccggcgggat ggcgccgccg gaaggcacga
     1561 tcgcggtgcg cctcgacccg ggcctggcct tcggcaccgg cacccatccg accactgcac
     1621 tgtgcctgga gtggctggac ggactggctg cgcccgcgcc gggcgaacgc tcgccgctgc
     1681 gcgatgcgct ggtggtggat tacggttgcg gctcgggcat cctcgccatc gcggcgttgc
     1741 ggctgggtgc ggccgcggcc atcgccgtcg acctggatgc ccaggcgctg ctcgccaccg
     1801 gcacgaacgc cgccgcgaac ggcgtggccg accggctgca gaccggtatg ccggcggagc
     1861 tttcggccat gctcgacggc cggaaagcgg atatcctcgt cgccaacatt ctggccggac
     1921 cgctgcaggc cttgctgccg cgattcgcgg cgacgctcag ggcaggcgga ttgcttgccc
     1981 tgtcgggtat tcttgtcggc caggaacagg ccctcttggc cgcagcggaa ccgctgttcg
     2041 gcatggatcc gccggcgatg cgcgaagact gggtgaggct ctcgggtcga aggaacgcga
     2101 aaggctgatg tttactcgtt gctccaattg caggaccgta tttcacatta ccgcggcgga
     2161 gttgcgggcg gccgacggaa ccgtggtctg cggcgcctgc ggcgcgacct tcgatgcgct
     2221 cgactcgctc agcgaggcgc gcccggagga accgccccac atccaccccg agcccgagaa
     2281 ggctgccgca ccgccgccgg tcagcgagag cgccgcggaa ctcgacgagc gggcgcgaga
     2341 cgaagacgag ttccttcagg aactcgagtc gttgatcggc ggagaggcat tcctggagga
     2401 gccctcgccg ggcgaagagc cgaccggcac ggggttcccg cagatggagt tctcgcgcgc
     2461 gccgttgcgc gacgaagacc tggccacgga cttgagcacc gcggatgagg cgcgcgggga
     2521 gttcgcggcc gaggacgaat tcctggtgga ggaattcgag gccgaggaca cgccggagga
     2581 atcccgcttc gatcaggcgc tgctcgccga tgagccggac gagcccggtg acgacgcgcc
     2641 ggcgcccggc gaacccccgc tgcgggacct cgacgaagac gacgacctgg acgatccctt
     2701 cctggatccg gactcggtgt ttcgtatcga cgaagaaccg gaagacggcg aaggcgcagg
     2761 agtttttgcc tggcgggacg ccgatcgcgc gtcgcgcgac acaagcgcag agggtgcagg
     2821 ccagccgcct gccgaggccg catcaccggg ttcggcggac gcggaagcgc ccgtccggga
     2881 cgcgccggag catgcagcgg ccgcaccggg gccggcgtcc gacgctgccg gcgaggcggc
     2941 cgtggagccg ctcccggagt tcgcccgcca accccgcccc ggcaggcgct ggctgcgcgt
     3001 cgtgacggcc ctgctggccg tgctcctgtt gaccggcacc tgggcccacg gccagcgcgg
     3061 caagttgctg cgccacccgg ccggcgaggc cgtactggga ccggtctacc gggcccttgg
     3121 catggagtcc gcaccggact ggaatccggc cgagtatcgc gcggtgcagt ggcaggccgt
     3181 cgcggacgcc gaccggcccg acgatctcgc ggtggcggtc gatttcatga acatggcgtc
     3241 ttatgcccag ccctatccca tcatccgcat cgtgctggaa gaccgcttcg gccgccgcgt
     3301 gggggttcac gacgtgccgc cctccgagta cctgcgcgat cattcccgcg gcagccggat
     3361 ggcgggcgga ggacgcttgc agacgaccgt cgtggtgcgc gaccccggcg gtcgggcgga
     3421 tggtttccgc gtcgacttct gcctcgagct ggagggacgc ggcctggtgt gcggaccggc
     3481 gccgttccgc taggccctgc aggtcgcccg acacgagcgg agcccccggg gaactgatga
     3541 aaatcggcaa ttactcattc gtagcacccg tcgtggcgct ggccccgatg gccggcgtca
     3601 cggaccgacc gttccgggcg ctgtgccgcc gcctgggcgc gagcttcgcc gccacggaaa
     3661 tggtgagcgc ggaccagcgc ctctgggaca cgcccaagag ccgccaccgg ctcgaccacg
     3721 cgggcgaagg ggacccggtc atcgtgcaga tcgccggggg cgatcccgag atgatcgcgg
     3781 cggcggcgct ggccaatgcc gcgctcggcg cgcaggtcgt cgacatcaac atgggctgcc
     3841 cggcgaagaa agtctgcaac cgcgcggccg gctccgcgct gctgcgcgat gaggggctgg
     3901 tacgggcgat cctcgaggcc gcggtcgcga ccctcgcgcc gctgggcatt ccggtcacgc
     3961 tgaagacccg caccggttgg agcccggaca atcgcaacgc gctgcgcatc gcgcggctgg
     4021 ccgagcaggc gggcgtcgcc gcgatcgcga tccatggtcg cacccgcgcc tgcggctacg
     4081 gcggcattgc cgaatacgat acggtgcgcg caatcaagct cgaggtgggc atccccgtca
     4141 ttgccaatgg cgacatccgg acgccggaag aggcgcgcgc cgtgctcgag cacaccggcg
     4201 tcgacggcct gatgatcggc cgggcggcac aaggcaggcc gtggatattt cgcgaaatgc
     4261 gcgccttcat cgccaccggg attcgctcgc cggagcccgg cgccgccgag gtccgtgata
     4321 tcatcctccg ccacctcgac gcattgcatc gcttctacgg tgaggcgcgc ggcgtgagga
     4381 ccgcgcgcaa gcacctggga tggtatcttg cggggcgtcc cggcggggag gaactccgcc
     4441 gcaggatcgt ccgcgtggag gatgccggcg agcagctcgc ggtggtgggg gaatatttcg
     4501 aagaagctct ggacaaagag ctcgcagcgt gacggggaat tacaacgtgt ccaataacaa
     4561 gacgcagcag gcgctgaacg gcaacggcaa gacgcggccg ggtggcgatg gcgcgctggg
     4621 cagcatggcc caggaagctc tcgaccagta ctttcagacg ctcaacgggc acaagccgga
     4681 ggagctttac gacctggtca tcggcgaagt cgagaagccg ctgttccggg ccgtgctcga
     4741 ttacaccggc ggcaaccaga gcatggcggc gactatcctg ggtatcaacc ggggcacttt
     4801 gcgcaagaag ctgcgcctgc acggcctgct cgactgagcg gccgcaggca gcggaacgac
     4861 ccggccatgg cgcagatcaa gcgtgccctc atcagcgtct ccgacaagcg cggcattgcc
     4921 gagtttgcgc acggcctggc cgagctcggc atcgagctcg tgtccaccgg cggcaccgcc
     4981 gcggcgctgc gcgcggccgg actgcgggta cgcgacgttg cggaactcac cggctttctg
     5041 gaaatcatgg gcgggcggat caagacgctg catcccgggg tgcatggcgg gctgctcggc
     5101 cggcgcggca gcgacgagga ggtcatggca cgccacggca tcctcccgat cgacctgctc
     5161 gcggtgaacc tctacccgtt tcgcgagacg gtggcggatc ctgactgcac catcgacgag
     5221 gcggtcgaga acatcgacat cggcggtccg gccatgttgc gcgcggcggc caagaaccac
     5281 gccgccgtca cggtggtcgt cgaacccgcc gactacgaga ccgtgttgac cgcgctgcgc
     5341 gccgccggcg aagtgcccta cgaaatgcgc ctgcggctgg cgggcaaggc tttcgcgcac
     5401 gtggccagtt acgacaccgc ggtgtccaac tggctcggct ggcaggcgga aatggacgcc
     5461 gcggagatgc cggacgatct cgtcctggcg gcccgcaaga cgctgcagct gcgttacggg
     5521 gaaaacccgc agcagcgcgc cgcgatgtat tcgtacggcc acccgcgcaa gggcacggtc
     5581 ggcgccgcgg agcagctgca gggcaaggaa ctgtcctaca acaacctcct cgatgccgat
     5641 gcggcactcg agtgcgtcaa gctcttcgac gagccggcct gcgtcatcgt caagcatgcg
     5701 aatccttgcg gcgtggcgac cgggcaagac atcctcgacg cctatcgccg cgcctacgcc
     5761 accgacccgg tgtccgcgtt cggcggcatc atcgccttca atcggccgct cgacggcggc
     5821 accgccgagg cgatcctgaa gaagcagttc gtcgaggtcc tggtggcgcc ctcgatcgac
     5881 ccgggcgcag ccggcgtgct gggcagcaag ccgaacgtgc gcgtgctcgc cagcgggcgc
     5941 tggagccggt ctccgctcgg cacgatccag ctgcgcagca tcggcggcgc gctgctcgtg
     6001 caggatgtcg acgacgccga ggtcgatacc gagcagttca aggtcgtcac gcagcgcgct
     6061 cccaccgagc acgagatgcg ggacctgctg ttcgcctgga aggtggtccg tttcgtcaag
     6121 tccaatgcca tcgtcttctg caaggacgcg gccaccatcg gcgtcggcgc gggccagatg
     6181 agccgtatct acagcacgcg catcgctgcc atcaaggcgg ccgacgccgg actcgccgtg
     6241 aaaggctcgg tgatggcctc ggacgcattt ttccccttcc gcgacaacgt cgacgccggc
     6301 gccgagcacg gcgtcaccgc catcatccag ccgggcggct cggtgcgcga cccggaggtg
     6361 attcgtgcgg ccgacgagca cggcctcgcc atggtgttca cgggcactcg ccacttccgg
     6421 cactgagccg gcccggcacc ggcaactgga cgcagcccga ggtagacgat catgaagatc
     6481 ctggtggtgg gaggtggagg ccgcgaacac gcgctcgcat ggaaggcggc gacctcgccg
     6541 ctggcggacc gggtgtacgt tgcgcccggc aatgcaggca ccgcgcgcga gcccggctgc
     6601 gagaacgtcg ccatcgatgc ggctgacatt gcggcactcg ccgatttcgc cgctgcgcag
     6661 gacatcggcc tcaccatcgt cgggccggag gtgccgctgg tcgccggcat cgtcgacgag
     6721 ttcacgcgcc gcgggctcaa gtgcttcggt cccaccgcgg cctgcgcgcg gctggagggc
     6781 tcgaaggcct tctccaaggc atttttcgcg cgtcacggca ttccgaccgc cgcctgggcc
     6841 gacttcagcg acgccgacgc ggcctgcagc tacatccgcg cgcagggcgc gccgatcgtg
     6901 gtcaaggctg acggactggc cgccggcaag ggtgtggtcg tggccgcgac ggttgccgag
     6961 gcggaggctg cggcgcgcga catgctgtcc gggaatcgct tcggcgacgc cggcgcacgc
     7021 gtggtcgtgg aggaattcct ggccggcgag gaagcgagct tcatcgccat ggtggatggc
     7081 gcgcacatcc tgccgcttgc ctcttcacag gaccacaagg cgcgcgacga tggcgaccgc
     7141 ggacccaaca ccggcggcat gggggcctat tcgcccgcgc cggtggtgga cgccgcattg
     7201 catgcgcgca tcatgcgcga ggtaatggag cccacggtcg ccgcgctggc ggccgacggc
     7261 acgccttaca cgggattcct ctacgccggc atcatggtgg cgccggacgg cgcgccgaag
     7321 gtgctggagt tcaattgtcg ttttggcgat cccgaaaccc agccggtgct gttcaggatg
     7381 aagtccgacc tggtcgcgct gtgcctcgcg gcgctcgacg gccgcctcga cgcggtcagc
     7441 tgcgagtggg acaggcggac ggcgctgggc gtggtgctgg ccgccgcggg ctatcccgac
     7501 gcggtccgca agggcgaccc gatttccggg ctggacggcg atgcggggcg tgacgacgtc
     7561 aagatcttcc atgccggcac ccgcctggaa ggcgaccggg tggtcaccag cggcggccgc
     7621 gtgctctgcg cagtggcgct cggcgacggc gtgcgcgagg cgcaacgcaa ggcctatgcg
     7681 ctcaccgcgc agatacgctg ggacggcgcc tttttccgcc gtgacatagg gtggcgcgcc
     7741 atcggccgtt gacccggcgc cgcagcggaa cccttagaat ggggttctcc tcattgcccc
     7801 tcggcggtaa ggccagtgac ccacgcggac ccgcgaccag cagcgcggcc ccttggcaga
     7861 gtctcacgac gtccggcgct cctggtgctg ctggcgacgg tatgctgcct gtggctcacg
     7921 gcggccgggg ccgaggtccg ggtcgacatc acgggtgtcg acggcgagat cgaggccaac
     7981 atccgcgcca ccctggacct ggtccggcac ggcgggcgcg aggacctgtc ggaggccgcg
     8041 gtgcgccggc tgcattcgcg cgcacctcgc cagatccgcg aggccatgcg gccctacggc
     8101 tattaccgac cgcgagtcca gccgtcgctc gagcaacgca acggtgagtg gcgcgcgcgc
     8161 ttcgtgatcg acgccggtga gccggtgctg ctgaaggaag tcgacgtccg catcctcggc
     8221 gacggcagcg gcgacggcgc gctgctggac atcgtcgcgc agagcccgct gcgcgagggg
     8281 cggcggctca atcaccagga atacgaccgg ctgcgcaacc ggctgcaggc gaccgccgcc
     8341 atgcgcggct acttcgacgc acgcttcgag cagcgccggc tggaggtcga cccctcggag
     8401 cacacggcgc gtgccgtgct gcacatgagc accgggcccc gctatcgctt cgggtcgatc
     8461 tcggtcgagc aggacatcat cagcgctgcg ctgctcgacc ggatcgtgct gctgcgcgaa
     8521 ggcgaaccct acgatgcgaa tgcgttgctg cgggcccagt accggctctc ggacacctcg
     8581 tatttcgccg gtgtggtagt cgagtccggc gcgccggacg aggcgaccct gtcggtaccg
     8641 atcaggatcg agaccactgc aacgcgcggt cagcgcatcc ggctcgggct cgggtatgcc
     8701 accgatacgc gcctgcgcgg cagcattggt gtggactggc gtcacctcaa caaggcgggg
     8761 cactcggccg ggaccgagtt gcgcctgtcc gaagtgctca ccgaaatcag cggccgttac
     8821 cgcattccga tcggcgaccc gctgcaggag cgcctgttgt ttcgcggcgg cctgacccgg
     8881 gaggacctcg ccgatatcga aagccagcgc atctcggtgg gggtcagcca cgtgaccatg
     8941 cgcggcggcg gctggcagcg caacctgttc gccgacctgc tcgaggaacg cacgcggacg
     9001 ccgaatgaac ccgagtttcg cgacctgctg atcgtgccgg ggatcggcat ggagaagctg
     9061 atcgcggacg atgtcctgtt tccgaggaac ggctaccggg tgcgcggcga agtgcgcggc
     9121 tcgcatcact atcttggcgc gcgtaccgac ttcctccgca tcatggccga ggcgaatcgc
     9181 gtcagctccg ccggtgaatc gtggcgcttc ttcctgcgca gtgcggccgg ctacggcatc
     9241 gtgggcaacg tcgatgcctt gcccgcctcg cagcgcttct tcgccggcgg cgaccagagc
     9301 gtgcgcggct atggcttcaa cagcctcggg ccgcgcgacg cggacggcaa cgtcatcggc
     9361 ggccggcacc tgctgttcgg tgccgtggag gcggaacgcc tggtctgggg acgttacgcg
     9421 ctggccgcgt tcctcgacgc cggcaatgcg ttcaacgact tcgacgaggg actcgaggcc
     9481 tccgtcggcg tcggcgtcaa cgtgcacacg ccgatcggca cgttacgagt cgccatggcg
     9541 cggtccatca ccgagagccg cggcatgcgt ttccacctca gcatcatgcc cgacctgtga
     9601 agcgccgcag gctcatcatc ccgatcgcgg tgctggccat gctcgccgcg gtcttcgcct
     9661 ggctcgtcgg cacgaccagc gggctgcgct tcctggcggc ccgcgcactg ccccacctgc
     9721 ccgtgtcgta ttcgccgggc gacgtcgagg ggcgcctgct tggcccgctg gcgctgggcc
     9781 cgctcgagct cgcggcgcct ggcctgagcg gcagcatcga gcgcatcgag ctggactggc
     9841 gaccgatgga actgtggcgc ggctccctgc acatcctgga actccaggtc cgacggccgt
     9901 tgctctcgct ggcgccgacc ggggatgttg cgcaggcacc gccgcaaggt gcgaccgggc
     9961 aattttccct gcccttgcgg cttgttgcgg accgccttgc ggtgatcgac ggtgaactcg
    10021 aggctgacgg cgaaactctc gtcgaagggc tgcagctcga gctggcggga cgggcctccg
    10081 gcagcacgct ggcactcgat cacgtggcgc tgcgcagcaa ccgcggcggc gtcacgggtc
    10141 atgcgcgtgc cggcatggcg ccggccacgc cgtgggacat cgacctggaa tggcaggcgg
    10201 tcgtggacga acaggaagtc gtcggccact cgcgcgtgac cggccggctc gcccgactcc
    10261 acctggtgca ggagatttcg gcgccgctgg tcgcacgcct cgagggcgtg gtggagggcc
    10321 tgcccgacgc gccggcctgg acgatcgagg cgagcaacga gccgctggcg ccggccgccg
    10381 gcccgtggcc ggagaccctg gtggggctgg ccacccgcct gcagcttgcc ggctcgcccg
    10441 acgacagcct gctgactggc aacctccagc tgccggcgct cgtggccggc accatttcgc
    10501 ttgcggcgca gggcggctgg caggaccatg cggcggtcgt gcggcgcctg gcgatcgtgc
    10561 tgccggacga cgcacgcctc gatgccgccg gccgcttcgt accgggcgaa gaacctgccg
    10621 ccgagttcag cctcacgggc gagcgactgc gctggccact ggacgccacc gagccggcgc
    10681 tggcgttgcc ccggctggcg ctgcacggca caggcgccga cgaccgctgg cgtttcacgg
    10741 ccgacgcgcg cgcggtgcgt gccggcctgc cggacgtcga cctcgtcgcc gagctgcagc
    10801 tgaccggcac gacgctcacc attgagcgcc tcacggccga ttccgcgggc ggcgaggtcc
    10861 acgcgcgcat cgccggactc ctcgagaccg ccaccgacga actccgctac caggtgaccg
    10921 ccgatgggca catcgagctg cctgattatc cgccggtcag cgcatcgttg aatgcaaccg
    10981 gcgatgtgtc aggcgtgaac atcgcggatc tcgatgcgcg gctcctcggg ggtaccttga
    11041 ccggcgcggg ccgcattgcc tgggagggcg aacagcaggc cgatttccaa ctggcgttca
    11101 gcggcatcga tccatccggc ctggcgcccg actggccggg gcggctcgac ggccggctcg
    11161 gcctgcgcgg cctgcccgac gctgcgagcg gcatggagat cgagctgggc gcgctgcgtg
    11221 gcgagctcag gtcgatgccg gtacggggcg aggcggccct caacctcggc cggtcagggc
    11281 aagtcctgcg taccctgtcg ctgtcgctgg gcgccgcctc gctgcaggcc cgcgggcagc
    11341 tcgacgacgc gtcggtggcg ctggaggcct cgctggaggc cccggaactg gatgcgttgc
    11401 acgacgatgc cgcgggcagc ttcagcgcat ccctcgagtt ggcgggcgct cgcgatcggc
    11461 cgcaggtgga gctcgaggcg cgcggggccg ggttgcgctg gcagtccggc cgcatccgcg
    11521 aactgtggat cgacgcgacc gtcgatccca cgggcgcgac ccgctcgagc ctgcgcgccg
    11581 cgctgaccgg cttcgcgacc gagcccggcc cgggcgcgag cctgaccctg gaggctgacg
    11641 ggacgccggc tgatcatgaa gcacgcctgg attatcaccg caggcggcag gggcagcggg
    11701 tccagctggc gctcgccggt ggcctggacg aggcgcgctg gatcggccag ctgcaggcat
    11761 tgacgctgac ggaggccggc caggaggcct gggcgctgca ggcgccggcg gatctgcgca
    11821 ccgccgccga ctcggttcag ctgaacaatg cctgcatgga cggcacgctg gggcggttgt
    11881 gcctcgatgc cgcatgggca aggggcggcc cctggcgcgg tcgctcagcc cttgcgcagc
    11941 tggacctgga gccgctcagc gactggctcg gccgcggcct gatcgctcgt ggcgtagtca
    12001 ccggccagct cgaggtcgaa gccgacgatg acaccttcct cggcctgtcg ggtggcctgg
    12061 cgctgaccgg cggtgacgtg cgcgtggccg gggaggacgg tgacaccctg ctggcgtggc
    12121 gctccggcgc cctcgagctg gccggcgacc gggacgaggc ggtggccgag ctcgatgtcg
    12181 tgctggccgg cgacgatcga atccagggcc ggctggcgat cggctggaac gaggcggatc
    12241 ccccgctgca gggtcgcctg gaggcccgcc tcgggcaact cgacatcatc accgagctgt
    12301 tgcccgacct ggcggagttg aggggcagcg cagtcgcgga agcggtggtg gccggcaccc
    12361 tcggggcgcc cgcaatcacc ggccgcttcg agtggctgga cggcacggcg gaaatcccca
    12421 cgctcggcct cgcgccgcga gacatccgcg tgctggccgc gttggccgag ggggtgctca
    12481 ctttcactgc gaccggccgc tccggcgatg gcgagttcga ggcggacggc cgcttcgacc
    12541 tcgccgcagc gggcgtcgag ggccgcgcgg cactgcgggg cgaggacctg ttgctggccg
    12601 acctgcccga ggcgcgcgtg accgccagcc cggacctgcg cctgcactat tcgggcaaca
    12661 acctcacggt cggcggagag gtccgcattc cgactgcccg catccgcggt cttggcggcc
    12721 ccggcgcggt gacgacgagc cctgacgaag tgatcgtcgg tccccgcgcc cgcaccgaag
    12781 acgagggcct cgaagtggcc agccgagttc gagtctcggt cggaccggac gtgcagatcc
    12841 aggccgccgg actgcgcggc cgtgtcgagg gcagcatcct gacggtgact cagccgcagg
    12901 cgctgccatg gggccgcggc gagctgcggg tcgtggacgg cacgttcggc gcgttcgggc
    12961 agcgccttga gatcgagacc ggtcgcctga tctacagcgg cggaccgctc gagaatccgg
    13021 gcctcgacat ccgcgcggta cggcgtatcg atacggtgac cgcgggggcg ctggtgcgcg
    13081 gcacgttgcg gcagccggaa atctcgctct actcggaccc gccaatgccg cgcgccgaca
    13141 cactttccta cctgaccctc ggcaagagcc tggacgagct gcaggccggc gaaaagcaga
    13201 cggtcaacca ggcggccagc tcgctggcgc tgtcgggcgg gggcctgatc gcccaggacc
    13261 ttgggcgcag gctgggattc gacgacgtcg cggtgactgc ggacgaacag accggcgggg
    13321 ccgccgtcgt catcagcaag tacctcggcg gcggcctgta cgtgagctat gggctcgggc
    13381 tgttcgatac cgtgaacacg ctgcggctgc gctaccagtt caaccggcgg ctctcggtcg
    13441 aagccgcttc gggcgagcaa tccgcggccg actttttcta caccttcgag cgcgactgat
    13501 tcaggcgtcc atcgccccac ggagctcatc gcggtgcgcc tgcgcccagg ctgcgaaatc
    13561 gcggatgtcc ggatacaggc ggcggctggc cgccaggtct tccggcttcg cgccgacgtg
    13621 gctgcggtac agctcgaaca tgtccgccag cgcggccgcg ccggggatcg gcagggcggc
    13681 gaaatcatcc cttgggacgt gctggtaccg gactgtccgt ccggtcgcgt gcccgatgat
    13741 gtccgcgtaa tccgccgccg gccgctgatc gccgacgatc tgcacggtcc tgccctggaa
    13801 ttcacgcggc gcacgcagga tcgcggcgat cacgccgccg atatccgcga ctgcaactgc
    13861 cgacagcggc gcgttgccca gtggaaaacc gaaggcatag ctgccgtcct gctgtcggcg
    13921 tggctggcaa ttgagcagga agttttcgta gtagaacgcg acgtgcacga aagtggcggt
    13981 gatttccttg ccgcgcacca gcgcctcgag tcgcgccttg tgctcgaagt gcggcaccga
    14041 cagcttcccc gacgacagct ggctggcgct gggcaatgtg ctgagcacca ggtgttcggc
    14101 tgccgaatcg accaccgcgt cgaggagacg ggcgccgagt tcgtactcgg cggcgaagtg
    14161 ctcgaaatag ttcgtcacgc cgaacaccgc ctcgcatccg gccagcgccg catcgagact
    14221 gtcgcgatcc gccatgtcgc cctgcacgac ttcggcgccc gcgtccgcga gcgcccgggc
    14281 ggcgaccgag tccagccggc gggtgaggca acgtacccgg caacgccctt cggcgagcag
    14341 gtgccgtgcc acgctgccgc cctgcgcgcc ggtgccgccg acgaccagaa tcctgggcgc
    14401 cggactcatc gggatgatcc tcccgtgccc ttcattcgcc gggggccgcg accgtgcccc
    14461 acgtaatccc gagcaccacc cagcgaccgt cccagcgcac cagctgcagg taatgcacgt
    14521 acagcggcga caccacccgc gcgacggcca tgtcgccatg gcgatccagg acggccaccc
    14581 tgagattcag cggcccgggg tccgccggct tgccatcgcc tgcgcgggtc gcacggatga
    14641 gctccgccgc ggccatctgc tgcacgcgcg gctcaccgct gaggatgtcc acgacgacgc
    14701 gacgcttgag cgcctgcgga tgcagggcgc gcgccatgcg ctcggcatcg gccgtggccc
    14761 acccctcggc atagtccagc gccaccgccc gtaccgcttc atcctcgtcc gcgctcgcgc
    14821 tcgccggtgc cccggccgct cccagcacca gcagtgcccc gcagagagcc agccggccgg
    14881 ctagaccggg cttggtcatg ttcaccctcc tcgacgccgc cctcgcggcg tcgcctgcaa
    14941 gcatagcggc cctgtgtcag gcgcgctcga acggtcgcaa ccgcgagatc gcgcccacga
    15001 tcccgatgat gcgctgcggt ggccactcct gcccgtccgg cagcctgccc cacaccaccc
    15061 ccggccagca gggatcgccg ggatggcgcc ccaccacgtg cacgtgaagc tgcggcacca
    15121 ggttgccgat tgcggcgatg ttgatcttct gcgcccccag ggagtctttc acgaagcgcg
    15181 ccacgacgtc gatctcgtcg accagctccc gccggtcggg agcctcgagc tcgaacagtt
    15241 cgtgcacggc agccgagacc tcgggcacca ggatcagcca cggcaggccg gcattgcggt
    15301 gcagcagcag atgactcagc cggaagcggc ccaggcggtg gcaatcggcc agcaggcggg
    15361 gatgaagttc gaactctgcc atcgggaaca tgccagcgct cgccggtgga gcttgcgctc
    15421 aaccggcgag gtcgccctcc gccgcctggc ctgcgagggc gtccaggtcg atcttgaaca
    15481 agttggccgc cgtgcgccag gcgatgcctt ccttggcctc ctccgagagt tccaggctgt
    15541 cgaagaagcg ccggtaggtg cgcatgccgg ccaacggcca gtcggtgccg tacatgagct
    15601 gcttgccggg gtcgcccatg tagctgatca tgtcgcgcac ccgctgcagc atgtagcgct
    15661 cgaactcgta atggaactcg cccagggtaa ggcccgagat gtcggcatag acgttgtcgt
    15721 tcttgtagag gacttcggcc gcatcctgga accagggatt gccgaggtgg cagatgacga
    15781 agcggacgtc cggataatcg acggcgacgt cgtccaccag cagcggatgc gcctggcgca
    15841 ccttggcctg cttggagtag gtgtcgccgc agtggatcat caccggcaca tcgtgtttct
    15901 cggcgatcct gaagacgctt tccagcgagg ggtcgttgat cgggtaacgg tcatagcccg
    15961 gatacagctt gatgcccttg accaggccct cgcggatacg ctcttccatg ttgtacaggt
    16021 cggtgcgctc gtcgggatcg cgccagcgca ggccctccac gatggtcacg cgcgggtcgt
    16081 cgcctaccac ccgaatgact tcatccacgt gcgggcgatg ggcgttgacc ttgtaggaag
    16141 tcagcaccac cgcgtggtcg acgccattct cgtccatcgc cgcaaacagg tcggcggctg
    16201 cctcgtccgc ggtgcggctg tgggtctcgt cgtagttgtt caggtgcaca tggcagtcga
    16261 tcagcatggc gctacactag cctgagacgg accgacagcc aagccacggc ggcctcgaac
    16321 ggtctcaaaa acttcttttc ccctgcacca ggaattgcac gctgtcgatc gacgcatcgc
    16381 cgtcgagcgg gaaggcgagg tcgatgtgga ccacgcggcc gatgctggaa cgcgtgctgc
    16441 cgagccgcag gccgaagccc acgtcgcgca gccagccgag gttctcgccg ccgtaggggt
    16501 tctcgcccca gacgcggcca gcgtcgaaaa atatcgctcc gccgacccgg aacaggcgcc
    16561 acaggtagta gtcggtgaag tatcgctgct cgatggtgaa ctgggcgcgc gcgtcgccac
    16621 gctggtagcg cagcggatag ccacgcaggc cgttgtcacc gcccagctcc agcggattgt
    16681 ccaggtccag gttctccgcc acgtcgccac gcacggccgc aaacaacagg cgccgttccg
    16741 actggcgcaa gtaccagctc gcggtaccac cgacgatggc gttggtgatc tcgccgcttt
    16801 ccagccggcc gctggcgtgg gcagaaaaat cccacagcac gtgcagtgga tcgccgtagc
    16861 cgcggcgcgc tacgacgccg aaggtcgcgc cacggcggtc ggcgccgagc gtttccgaca
    16921 gcaggccgag ccgcagtccg atcgacgccc cgagcgatac gtcctcggtg acgtagatct
    16981 ggtcgagatt cgccgcgcgc tggaagcggt cctcgatcaa ctggtactcg acgaagggat
    17041 agagcagctt gcgatcctcc gggatcagcg ccggatcgat cgacccgtcc gcgttggcga
    17101 accggcgccg gtcgtacacc acgccggcca gccagcgctg cacccagccg gcctgtaagc
    17161 cgttcgacca gcccccgtag atatcgaggt gctcgacgtc ctcgcggaac cgcccgatct
    17221 cgtcgccgag cgaatagacc ggatcctcga ggcgccggtc cagtatcctg gcgcccaggg
    17281 cccagcgcga gtcgagggcg taaaaagggc gctcgagacc gatgccgagc tcgtggccgt
    17341 cgctgttgtc cgcaacggtg gcgtccaggg aaatccagcg cccgccaagg ttgcggtccg
    17401 cgtagccgat ttccgtggag cggcgctcct cgtcgatccg acgcacgtaa cgcacgctcc
    17461 cgccgcgacc gaggaaattg tcctcctgca ggtcgaagcc gacccggctc tcgccgccgc
    17521 tgcgagacag cgagaagccc ggctttagcg tccagacgtc ccgggtatgc acctcgaggt
    17581 ccacggattg cgcatcacaa gcggcgatcc ggatgtcggc gtcatacagg taggcagtcg
    17641 agcgcagcag gcgctcggac tcgtcgagct gctgcacgcg aaaggcgtcg cccgatccga
    17701 acaacaactg ccgcgcgatc accccgtccc tggtgcgcac atgcaggcgg ttcatcgtcc
    17761 ggaacagcgc gttgtcctcg gccgggtcat cgaggtcgaa aatgttctcg gcgtggatgg
    17821 acaacttgcc gatgcgcgcg ccggcattct ccagcgcctc cggcgagaag ccgcaggccg
    17881 ccgccgcggg cggctcctcc ggttcagcca ggaccgggca ctgcaaggcg gcgccaacgg
    17941 tcagcagggc gagcatcccg gttcgccgtg tgtacatgtc agggcccgcg ggcaaggacc
    18001 gggggggtcc tgtcaggtaa ttccattgcc atagactacc gcgatttggc gcccgcgggc
    18061 gccggatggc cggcatggcg gcgccggtcg ttacaaccgc gaattgacgc gggcgcccag
    18121 cgcagggcag cgtagtgagc atgtccagga acacgccgac accgccggga gcggccgggg
    18181 cgaggccatc gcccgatgac cgggcccgca tattgcgagg tatcggcggt cccgccctgc
    18241 tggtcctgta cctgctgatc gcactcgcgc cgctggcgct ggcggcgctg ctcggcgccc
    18301 ctgcccggaa tccctggcgc gaactggcct cggggctggc gatggtcggc ttcgccatgc
    18361 tgttgctgga attcgtgctg tcgggccgtt ttcacggcgt ctcgggtcgc gtcgggatcg
    18421 acgtcaccat gcgggtacac cagctggctg cgctgggctt gttgctgttc ctgttcgcgc
    18481 atcccttcct gtacgcggtg ccgcggctca gccccgaccc gatgcgcgcg gcgagcttcg
    18541 tgcagcgcat gttcactggc gagtggtatc gctccggggt cgttgcgtgg tgcctgctgc
    18601 tggcgctggt accaatggcg atctttcgcg accggctgcc gtttcgttac gagatctggc
    18661 gggcgagcca cgggctcggc gccgcgctga tcgccggcct cggcctgcat cacacgctca
    18721 ccgtcggcac gcacggcgcc catcccgtgc tggcggggtt ctgggtcgtg atgacgggcc
    18781 tggccgtgct caccttgctg ttcgtttacg tgctcaagcc gctgtcgcag ctgcggcgac
    18841 cgtatcgcgt ggtcgccaac cggcaggtgg ccgaccgctg ctgggagctg gtggtgcgcc
    18901 cggatcacga ccagtcggtg catttcctcg ccggccagtt cgcctggctg aatctcggcc
    18961 accatccgct gagcctcacg gagcacccct tctccatcag ctcggcaccg gccggccggc
    19021 ccgacgtcgc cttcaccatc aagcagagcg gcgacttcac caaccgtatc ggcgaagtgc
    19081 cggtgggcac gcgggcctgg ctggacggac cacacgggca cttcgtgctg ccggatccgc
    19141 accccgaccg gattgccttc atcgccggcg gcgtgggctt tgcgccgatc atcagcatgc
    19201 tgcgccagct gcgtgcagag cgctggcgcg gccgcgccgc cctggtgtac ggcaatcgcg
    19261 tcgaaaccca gttgctgtat cgcgaagagc tccggcagat ggcgggcgaa ttgaacctcg
    19321 agctgcatct cgtgctgtcg gagccgccgc cgggctggac cggcccggtc ggcgaactcg
    19381 accgggagat gctggacgcc tgtctcgacc cggtggaccg ggccggatgg gtctacttcg
    19441 tgtgcgggcc tccccccatg atgaacagcg tcgagcacag cctgcgcgat tttggcgtcc
    19501 cggcccggca gatcgtcgcc gagcgcttca agtacgactg aggtggagcg gtgaggctcg
    19561 cttgatgcgg tggactgaaa gccattaggc tcgcacacca tgcccacctc gctgatcatc
    19621 gtggacgatt ttctcgacaa cccgcagcag ctgcgcgagg cggcgctgcg actgacctat
    19681 ccgcaggttc agggcgcctt cccggggcgg aattcggtcg agcgcatcga catcggcggc
    19741 ctggcgaacc aggtctcgcg gctggtcggc gagcatgtcg agccgatgcc gccgccgcag
    19801 tcgcacggca agtgccgcat cacgctcgcc agtgacgtcg gcaaggcgaa ggtgcatgtc
    19861 gatggcgccc actggtccgg gattctctac ctgagccggc cggaggactg ccgcgggggt
    19921 acggaatttt tccgccacaa ggcgaccaac accgaccgcg cgccgtacga cgaccgggaa
    19981 gccgcgggcc atggctttgc gtcggcacgg cagatggtga ccgagatcct cgaccgcgac
    20041 tcccgcgacg actcgaagtg ggaaatgacc atgcgcgtgc cgatgcgctt caaccggctc
    20101 atcctgctgc gcccctggct gtggcacacc gccggcgaga atttcggtga ctcgctgcag
    20161 aacggtcgcc tggtctacct gatgttcttt gccatggcgg cgggccggtg acggcacgct
    20221 ccctgacccg gctctgcatg gccgtgatgg tcgggttgct gctggcggtc gtgctctatg
    20281 ccttgcgata aatagatatt gtctatcgaa tcgagagaat taattcattg tactaatgga
    20341 tgcgcgcgcc ttatcgtgcc atccaaggca attccccctg gagggcgcaa ccatgaccaa
    20401 gacactcagc ttcgccgtgg tgcatttctt cgtcgcgttc agcgtcggct tcgcgctgac
    20461 cggcagcgtg gccattggcg gcgcgatcgc cctcatcgag ccggccctca acaccgtggc
    20521 ctattttttt cacgagaagg cctgggaccg catccgcgcg ccgcggcacg ccgtttcgcc
    20581 gcagcccgct tgatgccggc ccgccctccg gcggcataat cgggccatga cggccctcga
    20641 caacgccacg ccggccgccg cgccgaagga actgatactc agcgtcggcg ggatgcgctg
    20701 cgcctcctgc gtcggccggg tcgagcaggc cgcgcgcaag gcccccggcg tgaccggcgc
    20761 cacggtgaac ctcgcctccg gacgggcctt cgtgacgctg gccgacccgg ccgcgaacgc
    20821 cgaggccgtc gcccaggcga ttcgcgacgc gggattcgag gccgagccgc tgctcgacgc
    20881 cgcggagcag gcatcacgcg acgataccgc gagattcgac gagagccgcg cgctgcgcaa
    20941 cgcgttcttg ctggcggcgc tgctcaccct gccgatcttc gtcctggaga tgggcgcgca
    21001 tctcgtgccg ggcatggccg gattcgcggc cgatgccgtc ggcatccgca acctgcactt
    21061 gctgttcttc gtgctggcga ccgcggtgca gttcgggccc gggctgcgct tctaccgcac
    21121 cggctggccc gcgctgatgc gcggtgcgcc cgacatgaat tcgctggtga tgctgggcag
    21181 cacggccgcc tgggcctatt cggtcgtggc ggtgttcttc accggactgc tgccggccgg
    21241 caccgcgaac gtctatttcg aggcatccgc ggtcatcatc accctgatcc tcgccggtcg
    21301 ctacctcgag gcgcgtgcgc gcggccgcgc cggcgacgcc atccgccgcc tggtgtcgct
    21361 gcagccccgc acggcgcgca tccagcgcaa cgggcgcacc ctggaagcac cgatcgagcg
    21421 cgtgcgggca ggcgacctgg tgctggtgcg cccaggtgaa cgcgtgccgg tcgacggctg
    21481 cctggtggag ggcagttcct gggtggacga atcgatgatc agcggcgagc ccctgccggt
    21541 ggagaagtcg gtcggtgacg aggtgatcgg cgggaccatc aacggcagcg gcgccttcac
    21601 cttccgtgcc acccgcgtgg gcgcggaaac cgtgctggcg cgcatcgtgc gcatggtcga
    21661 gcaggcgcag gcggccaagc tgccgatcca ggccctggtg gaccgcgtca cccacgtgtt
    21721 cgtgcccgtc gtcatcgggc tcgcgatcct gactttctcc gcctggctgg tgctcggccc
    21781 ggcgccggcg ctgggcttcg cgctggtcaa cgccgtcgcg gtgctgatca tcgcctgccc
    21841 ctgtgcgatg ggactcgcca cgcccacctc gatcatggtc gggaccggca aggccgcaga
    21901 gctcggcctg ctgtttcgca agggcgatgc gctccaggcg ctccaggaag cggacacggt
    21961 ggcgctcgac aagacgggca cgctcacgcg cggcgagcct gcactgacgg tgttccggac
    22021 ggccccgggc ttcgacgaga cccgcacgct ggccctggtg gcgtccgtcg agcggcgctc
    22081 cgagcacccc atcgcggcgg ccatcgtcgc cggcgcgcat gatcgcggca tcgccctgca
    22141 cgacgcgacg gagttcctgg ccgaggccgg gctcggtgcg agcgccctgg tcgaggggcg
    22201 gcgtgtcgca gtcggtgcgg cgcgtttcat ggcgcggcag ggaatcgacg tgacgacttt
    22261 tacggcagcg gccgccgagt tcgcggccga aggcgcgacg ccgctctacg ccgcggtgaa
    22321 cggacgctgc gcagcgctgg tggcggtggc ggacccgctc aaggaatccg cgcgggtcgc
    22381 gatcgcggcg ctgcaccgcc tcggcctgcg cgtcgtcatg ctgacgggcg acgaccgtcg
    22441 caccgccgcg gcgatcgcgc gcgaggcggg catcgacgaa gtgcacgccg agctgttgcc
    22501 ggacgacaag gtaaaggtgg tgcgccgctt gcaagccggc ggccgcaagg tggcgttcgt
    22561 cggcgacggg atcaacgatg cgccggcgct cgcccaggcc gacgtcggca tcgccatcgg
    22621 caccggcacg gacatcgcca tcgacagcgc cgagctggtg ctcatgtccg gcga
//
LOCUS       DAOMPG010000008        20728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1108_length_20728_cov_5.020171, whole genome shotgun sequence.
ACCESSION   DAOMPG010000008 DAOMPG010000000
VERSION     DAOMPG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 20728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20728
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_20728_cov_5.020171"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLS34_00900"
     CDS             complement(<1..968)
                     /locus_tag="PLS34_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRP86061.1"
                     /translation="MSNSFRAFRIHQAGKGVEAKLETLSLDDLAPGEVVIRGLYSSIN
                     YKDALAATGKGRILRRFPLVGGIDVAGEVVSSEDERYRPGDQVVVTGCGLSEEHDGGY
                     AEYARVRGDWVIPIPAGLDARATMQLGTAGFTAGLAIHRLEHNGLSPERGPVIVTGAT
                     GGVGSLALDMLAGSGYRVTALTGKVEAAGYLRKLGAAEVLVRGEFEMGTRPLEKATWA
                     GAVDNLGGETLAWLTRTMDWWGSIASIGLAQGHELHTTVMPFILRGANILGVNSVATP
                     RALRLAVWARLAGTLKPRHLDTIAHRAVSLSELPGVFDEYIDGTVRG"
     gene            complement(965..1939)
                     /locus_tag="PLS34_00905"
     CDS             complement(965..1939)
                     /locus_tag="PLS34_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP86062.1"
                     /translation="MHKPSLIEFDAGITAVDTEYVRPGLDASHLVVHGDRAAFVDTGT
                     SRSVPLLLAALEAKGLEPAQVDWVFVTHVHLDHAGGAGELMAAPMAALPNATDVLHPR
                     GARHMADPTRLIEGSRAVYGDEAFDALYGEIKPIPEARIRTVEDGDRLALAGREFEFI
                     HTEGHAKHHYCIVDPMAAAVFTGDCFGISYRELDTAAGPFIFPTTTPVQFDPEAAHAT
                     IRRLLSYRPEHAFLTHFSRVAAPAALAPQLHRDLDEYVRIATARRDAADPEASIRDAL
                     RDWTFARLDEHGFDPDPALREEILNMDMALNAQGLAVWLANSARGERS"
     gene            complement(2068..2406)
                     /locus_tag="PLS34_00910"
     CDS             complement(2068..2406)
                     /locus_tag="PLS34_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP86063.1"
                     /translation="MSRTSYLRGLAALAAGLLLAGTAQAGNPELGRAKSAPCAACHGA
                     DGRGIAPNFPVLAGQYADYLAKSLKQYRSGERSNALMAPLAAGLSDADIADLAAYYAS
                     LPGLTVPKKR"
     gene            complement(2403..2714)
                     /locus_tag="PLS34_00915"
     CDS             complement(2403..2714)
                     /locus_tag="PLS34_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP86064.1"
                     /translation="MQSRAVLFGLALAGLLAATPAAADSRPDAVETCLGCHAIPGYKN
                     AYPTYHVPKLGGQHAQYISDALRAYASGERPHSTMHANATTLSEEQIAAIAKWFEEQG
                     Q"
     gene            2875..3585
                     /locus_tag="PLS34_00920"
     CDS             2875..3585
                     /locus_tag="PLS34_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRP86065.1"
                     /translation="MLHLRILLLALLLGLPAAALASADETRPTVTASGEGRVEVAPDI
                     AWLNFGISARQPTVAAARDEVARGVARLIELARRQGLKDEHIATAALSVSPEFDWHPE
                     TRERRLLGYVVSRQVTLRLTDLAKLGELTEQALGLGVTDASPASFDTSRRAELEAEAL
                     AAAARDARSRAEVMARALGVAVGAPLRIEAGSSGVSPRPMMRAAMEADAMSGAETYQP
                     GLIRVTASISATFLLLNP"
     gene            complement(3629..5233)
                     /locus_tag="PLS34_00925"
     CDS             complement(3629..5233)
                     /locus_tag="PLS34_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86066.1"
                     /translation="MSRAAAGWLLAVLGAAFLLGWLVLRPDAPSLPGARSAAVEFVRG
                     NNADPESLDPLQARSEPALNILRDLHEGLTTLDASAQVVPGAARAWETSADGLEWRFE
                     LEPAARWSDGRPVTASDFVTAFQRLADPVTASPHVGLFDAVAGLAEAAAGRSAVAEVG
                     VTADAPDRLRIRLLRPTPWLPELLAHPAASPRRDDLPADGRVSSGAFRFVEQVPGSHL
                     LLARNPEYRAAASLAVDRVRYLPIADLASELSRYRAGELDMSYTVPVSRIPWLVENMP
                     EEFRVSPYLAVYFLGFNTRRPPLDDARLRRALSLVVDRELIAARIVGAGEVPACSLVP
                     PGIPGYAPPRDAECAARRRERVERAQALFAAAGYGPEQPLRLEIRYNTGELHDRIAVA
                     VSSMWKEALGVETSLVREEFRVLLANVRAGEVTQVYRGSWIADFAEPESFLGILAGDS
                     PVNGTGWADPVYDRLLREAASQSDRAARLRLLARAEARALEAAPLLPVYFYVSKKLVK
                     PHVHGIEDNPLNIHPSRFVRIVRDPD"
     gene            complement(5235..6869)
                     /locus_tag="PLS34_00930"
     CDS             complement(5235..6869)
                     /locus_tag="PLS34_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86067.1"
                     /translation="MSTPRSGAWLAAPCLAAMLAAGCGQGAGPEPAAQAAAAPVVFRR
                     ANGPEPDTLDPQRAEDESSREIIRDLFEGLVGETPAGELVPGAAESWQVSPDGLSWTF
                     VLRADGRWSNGDPLLAEDFVAGLRRAVDPATASSSAALLAPLESATEIIAGRLPPERL
                     GVAALDARRLQLRLQSPTPYLLGLLTNPITFPVHRPSLAEHGASFARPGRLVSNGAYR
                     LDAWEVHSHVQLLANPHFREPPAIGVVRHYAFDAPEAALNRYRAGDLDFSMQMPMARF
                     QWLRDNLPDELHTAPAISTQFWLFNTVRAPTDELRVRQALSMTVDRQRIVDTVTGLGD
                     HAAYGLVPPGTANYTAQRHEWHDQPIAARAAAARELLAAAGYGPARPLRVEVHYNTDE
                     NLRRIAVAVASMWREHLGVETVLHNEEFRVLLSRRKHPDQWQVLRLSWRGDYNDASNF
                     LEIISRHGAVNDTGYDDPEFERLLAAAAAELDPAARRRLLETAERRMLEHYPVLPLYH
                     PVNKALVKPWVRGFSGNVLGRTYTRHLSIDTAQRGY"
     gene            complement(6866..8515)
                     /locus_tag="PLS34_00935"
     CDS             complement(6866..8515)
                     /locus_tag="PLS34_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86068.1"
                     /translation="MATAGRPGCVARTLWLALLLAVAACGQPQESADGVVLKPGVQVL
                     KRGNGSEPLTLDPHRAVSLSSLNILRDLFEGLVGTAPDGTPVPGVAASWETSADGLRW
                     QFALRPDARWSNGEPVTARDFVAGIRRALDPATASGSAGLLAPILNARAVMTRRMAPE
                     ELGVEAPDDLTLVIHLVAPTPWLLDLLTHPVSSPLYRPGLEQHGEDFARAGRLVSNGP
                     FYLADWQPRHSITLARNHNYWDRPRVAIDQVIYYPIEDQDVELERYRDGKLHWTSDVP
                     HHQLGLVRRRLGDELHITPRLAVTWLGLNVTRPPFAGSPGLRRALALAIDRELIVRSV
                     TGAGEVPAYSWVPPMAGYAAQTPTWASLPRRRQVEEARRLFEAAGFDAARPLELELLY
                     PGGLNNRRLAIAVAAMWRETLGIQTRLREEPFGEFLVSRNDLGTTMVFRSGWAADYRD
                     PYSFAGLFDSETGGSDTGWRNVQYDELLQASMQARNSRRRLALLGEAEQLLLDEQPII
                     PLYFDVRRRLVKPEVRGWEPNPMDLHPSRHFYIAVAEAGPQ"
     gene            complement(8520..8651)
                     /locus_tag="PLS34_00940"
     CDS             complement(8520..8651)
                     /locus_tag="PLS34_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86069.1"
                     /translation="MAEDAARPATLGQAARLPGVTPAAVSLLLVHLKRRGRADGEVA"
     assembly_gap    8717..8726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8727..>9887)
                     /locus_tag="PLS34_00945"
     CDS             complement(<8727..>9887)
                     /locus_tag="PLS34_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP86070.1"
                     /translation="ARSLDYGQLREQPGDEPRPVFSFLGRREDHPRQVPCWITATTER
                     THDIIRAALHRSPMYSGTIEGVGPRYCPSVEDKVTRFADKTSHQVFVEPEGLDSLEVY
                     PNGISTSLPYDVQLELVRSIPGFERAHLTRPGYAIEYDYFDPRDLRPHLETRHFRGLY
                     FAGQINGTTGYEEAAAQGLLAGVNAVLGLRGEAPWVPGRHEAYLGVLVDDLVTRGTAE
                     PYRMFTSRAEYRLLLREDNADERLTPRGRELGLVDERRWRDFERRQAEIEGETRRLAA
                     CMVRPAALTPAEAAAFPGGVPTREAPALELLRRPEVGHADLVALGCVGDAGVAPDTAA
                     QIEVRCRYDGYIRRQHLEVERHRRQETTRLPDDIDYQQVPGLSHEVRQKLRA"
     assembly_gap    9888..9938
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9939..10489)
                     /locus_tag="PLS34_00950"
                     /pseudo
     CDS             complement(9939..10489)
                     /locus_tag="PLS34_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782869.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(10509..12299)
                     /locus_tag="PLS34_00955"
     CDS             complement(10509..12299)
                     /locus_tag="PLS34_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRP86071.1"
                     /translation="MAQSADVFISYSREDKDKVLELTSKLSAAGVPIWMDVGGIDGAT
                     LWGEEIVHALEKAKVLLLMVTESAVRSHNVAKEVVIASERKGHILPVHLEPTRIPPSF
                     RYPLAGIQHIEYFQGDPEENLKTILRSLERIGVELKAPAKPAAPDAGAKLRSIKAAEA
                     PVTAEQSIEPGALAVLPFDNISPDEETDYFSDGLTEELIARLSLVSEIDLISRWASMQ
                     YKGVKQDIRVIGSELGARYIVGGSVRRFRDSVRVTVQLVDVETNRQLWGNTYKGKLDD
                     IFDIQEQVAKQIVEALKLQLSFSEKLSLTKRQTVNAQAYDFYLRGQDYLYRLTKRSVE
                     YAIQLFEKAIELDPRYAAAYAACSSAYGQMYQYFSRKEIYRDKAQEMSFKALMYDNNL
                     PEAYTAMGLSYFIWGKFDEAAESGRKAIELDPDDFIAYWTLGRIHFSTGKTQEAGDLF
                     RRVIELKPRFYVAHADLRMACQALGQVKESLELAHKLAEELLPNYLLQNPDDSRARMF
                     YAIMLSEVDRREDAIREGAAALEMSPGDSVMLYNGACLYAQLGEKRRALDTLREAITA
                     GVENFGWIKHDTDLAALRDDPEFLEMVERK"
     gene            12570..13766
                     /locus_tag="PLS34_00960"
     CDS             12570..13766
                     /locus_tag="PLS34_00960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP86072.1"
                     /translation="MGTPLQDRRRAEPAAAMDARQAVRLRRFYMALASYALWATIALI
                     AWAGNLIVMPLWLLGATLAGVVLTNAWFWFQLRTGRSLLYEDPSMTLQQVALALFWLL
                     VIIGASQADRSLMMVVYIVVMLFGIFRLEQRHFLVLTGIALVGYAVVVSADILLRGVA
                     VSLFEELVRFLVLASCLLWCSFFGTHVAQLRARLRQQNEELQRHVQQVQRIMDRDHLT
                     WAYNRHYIMNRLDEECARSEHRNAPFAIVIFDLDHFKSINDRYGHIAGDRVLKKFSEV
                     ARRELRAADVISPGRRSNSFGRFGGEEFIAVLPETGLDGARLCARRLQTALSTEQFES
                     DIRVTFSAGIAIHRHGESVEQTLRRADDALYRAKRAGRDQILEEKPARKKPRAPNIVP
                     LASKGG"
     gene            complement(13774..15138)
                     /gene="mnmE"
                     /locus_tag="PLS34_00965"
     CDS             complement(13774..15138)
                     /gene="mnmE"
                     /locus_tag="PLS34_00965"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRP86073.1"
                     /translation="MSASADSDTIAAIATAPGRGGVAVLRLSGPSATELAATVAGSLP
                     PARQAALRVLRDADGEIIDRALLLTFPGPHSFTGEDVIEFQLHGSPVIAAMLLQRLVS
                     LGAREAGPGEFTRRAFMNGRLDLAQAEAVADLVASASTAAARAALRSLEGEFSVAVHA
                     LVEALTLLRVRLEAGLDFPEEGVDAFTDPALAGELQDLLGRLATLRAAAERGRVLRDG
                     LTLVIAGAPNAGKSSLLNRLAGYEAAIVTELPGTTRDLVREDILIDGMPVRVVDTAGL
                     RDAADRVEAEGIRRAHDAMHRADLVLLVQDCSAAAAPGTAPASAPALPADVPVLRAWN
                     KIDLAGLPPGQGRDADGNEVLRLSALTGAGIELLRARLKAFAGYGDGVEGSFAARRRH
                     LDALDRARARLRQAGALLAGGQAVELAAEELRLAQAALGEITGEFTTEDLLGRIFSEF
                     CIGK"
     gene            complement(15149..16813)
                     /gene="yidC"
                     /locus_tag="PLS34_00970"
     CDS             complement(15149..16813)
                     /gene="yidC"
                     /locus_tag="PLS34_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRP86074.1"
                     /translation="MDNQRVFLWIALALLVWLNYMAFIEYQRPAAPPPPVDERLAPAL
                     PEEATVPALPSGNEIPVPPGGAVAAAADDAAAPAAEERRVRVATDVLELEISLRGGDL
                     VKALLPTYPVHKNQPDVPVQLLDPTRDGLFVFRAGLTSTAAETSTRPDTEFVAPAMEY
                     RLAPGQDAIEVPLTARLDNGVTVTKTYRFTRGSYAVQLEQQVANEGEAPWTGASYLQI
                     RRLHNPPNRSMMNVESYSFRGPVTFNGEKYEKRNIDDLAKSRVEFTTEGGWIASIQHH
                     FLAAAVPAGRAQFTASANTDGLYTLSAVGMPAVVAPGQTSIFTDQLFVGPKLQDQLQA
                     TAPRLRLTVDYGWLTVISQPLFWVLSKIYGLVGNWGWAIVLMTVLIKAVFYKLSETSG
                     KSMAKMRKLQPRMKALQERYADDRQKLSQAMMELYKKEKVNPAAGCLPILVQMPVFLA
                     LYWVLLESVEMRQAPFMLWITDLSVRDPLFILPLLMGVTMWIQQKLNPAPPDPVQAKI
                     MQFLPVVFTVFFAFFPAGLVLYWFVNNLLAITQQWHINKVVERGAA"
     gene            complement(16831..17058)
                     /gene="yidD"
                     /locus_tag="PLS34_00975"
     CDS             complement(16831..17058)
                     /gene="yidD"
                     /locus_tag="PLS34_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRP86075.1"
                     /translation="MRSIAIFLIRGYQLLLSPWLGNNCRYQPTCSQYAIEAVQAHGVV
                     RGLWLASRRIGRCHPWGGHGHDPVPPRRPIA"
     gene            complement(17034..17375)
                     /gene="rnpA"
                     /locus_tag="PLS34_00980"
                     /pseudo
     CDS             complement(17034..17375)
                     /gene="rnpA"
                     /locus_tag="PLS34_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            complement(17449..17583)
                     /gene="rpmH"
                     /locus_tag="PLS34_00985"
     CDS             complement(17449..17583)
                     /gene="rpmH"
                     /locus_tag="PLS34_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRP86076.1"
                     /translation="MKRTYQPSNLRRKRTHGFRARMATKNGRQVLSRRRAKGRARLAV
                     "
     gene            17785..19140
                     /gene="dnaA"
                     /locus_tag="PLS34_00990"
     CDS             17785..19140
                     /gene="dnaA"
                     /locus_tag="PLS34_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRP86077.1"
                     /translation="MAAVWHQCLSKLRSELSPDQFNTYVAPLQAVREGEALRLLAPNP
                     YVVSWLRQNCLARIRELTADEASGRGPVEVSIDVGTRAPDMPAGAAAPRESAAPTPAG
                     PTVPRGEFVTGARLNPDFTFESFVEGKSNQLARAGAFQVAANPGGSYNPLFLYGGVGL
                     GKTHLMHAIGNAILQRNPGARVAYVHSERFVSDMVRALQNNRIDEFKRSYRSLNALLI
                     DDIQFFAGKERSQEEFFHTFNALFEERQQIVLSCDRYPKEVDGLEDRLKTRFGWGLTV
                     AIEAPELETSVAILMKKASFEGVTLPSEVAFLIAKRIRSNVRELEGALRRVIATSRFL
                     DREITMDFAKKALGDLLSLQDRLVTIDNIQKTVAQYYKIRVADLMSKRRNRAVARPRQ
                     IAMCLAKELTERSYPEIGDAFGGKDHTTVLHACKRIKELRETDMRVREDYANLLRTLT
                     A"
     gene            19357..20457
                     /gene="dnaN"
                     /locus_tag="PLS34_00995"
     CDS             19357..20457
                     /gene="dnaN"
                     /locus_tag="PLS34_00995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRP86078.1"
                     /translation="MKFSAPREALLEPLQAVIGVVERRQTMPVLANVLLIAGEKTGLS
                     VTATDLEVELVATVDVQVEKGGEITVPGRKLLDICRALPDGAAVSFSLSGDKVTVRSG
                     RSRFTLSTLPASEFPTVEDIRAQQAIRLPQGQLRRLIEKTQFSMANQDVRYYLNGMLL
                     EVAGPLIRSVATDGHRLALCELSLDKADSSPQQVILPRKGVLELQRLIGEGGDCSLEI
                     GTNHLRVTLDGIRLTSKLIDGRFPDYDRVIPKESDAPMLADRVELADGLNRIRILANE
                     KYKGVRVTLKPGSVTVQAHNPEQEEAEEEIEVQYDGAEMEIGFNVEYLIAALRALDDD
                     KVRLEFRDAASSCLIQDPERRDCKYVVMPMRL"
     gene            20538..>20728
                     /locus_tag="PLS34_01000"
     CDS             20538..>20728
                     /locus_tag="PLS34_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRP86079.1"
                     /translation="MNLVTGMNASGKTSLLEAIFLLGRGRSFRAARREAVIREGAEQL
                     RVVARLADGRMLGIEAGRGS"
BASE COUNT         3141 a         7101 c         7218 g         3207 t
ORIGIN      
        1 ccgcgcaccg tcccgtcgat atattcgtcg aaaacaccgg gaagctcgga caaactgacg
       61 gctctgtgcg cgatcgtgtc gagatgtctc ggcttcagcg tacccgcgag ccgcgcccag
      121 accgccaggc gcagtgcccg cggcgtggca acggagttca ccccgaggat attggcgccc
      181 cgcaggatga agggcatcac ggtggtgtgc agctcgtggc cctgggcgag gccgatgctg
      241 gcgatcgagc cccaccagtc catggtgcgg gtcagccagg ccagcgtctc gccgcccagg
      301 ttgtcgaccg cgccggccca ggtcgccttc tccagcggtc gcgtgcccat ctcgaactcg
      361 ccgcgcacca gcacttcggc ggcgccgagt ttccgcaggt agccggcggc ctcgaccttg
      421 ccggtcagcg cggtgacgcg gtacccgctg cccgccagca tgtccagcgc caggctgccg
      481 accccgccgg tggcgccggt gacgatcacc gggccccgtt cgggggacag tccgttgtgc
      541 tccagccgat ggatggccag cccggcggtg aagccggcgg tgccgagctg catggtggcg
      601 cgcgcgtcga ggccggcggg aatcgggatg acccagtcgc cgcgcacccg cgcgtactcg
      661 gcgtagccgc cgtcgtgctc ctcgctcagg ccgcagccgg tcaccacgac ctggtcgccg
      721 ggccggtacc gctcgtcctc cgaactcacc acctcgccgg cgacgtcgat gccgccgacg
      781 agcgggaagc ggcgcaggat gcggcccttg ccggtggcag ccagggcgtc cttgtagttg
      841 atgctggagt acaggccgcg gatgaccacc tcgccgggcg cgaggtcgtc gagcgacagc
      901 gtctccagct tcgcctcgac ccccttgccc gcctggtgga tgcgaaatgc ccgaaatgaa
      961 ttgctcatga ccgctctccc ctggcgctgt tggccagcca caccgccagg ccctgggcgt
     1021 tcagcgccat gtccatgttg agaatttcct cgcgcagcgc cgggtccgga tcgaagccgt
     1081 gctcgtcgag gcgcgcaaag gtccagtcgc gcagcgcgtc gcgaatggac gcctcgggat
     1141 cggcggcgtc gcggcgcgcg gtggcgatcc tgacgtactc gtcgaggtcg cggtgcagct
     1201 ggggcgccag cgccgccggc gccgccaccc ggctgaagtg cgtcaggaag gcgtgctccg
     1261 gccggtacga caacagccgc cggatggtgg cgtgcgcggc ctccgggtcg aactgcaccg
     1321 gcgtggtggt gggaaagatg aacggccccg cggccgtgtc cagctcgcgg taggaaatgc
     1381 cgaagcagtc gccggtgaac accgccgccg ccatcgggtc gacgatgcag tagtggtgct
     1441 tcgcgtggcc ctcggtgtgg atgaactcga actcgcgccc cgccagcgcc agccggtcgc
     1501 cgtcctcgac ggtgcggatg cgggcctccg ggatgggctt gatctcgccg tacagcgcgt
     1561 cgaaggcttc gtcgccgtac acggccctgg agccctcgat cagccgggtc gggtccgcca
     1621 tgtggcgcgc gccgcgcgga tgcagcacgt cggtcgcgtt cggcaacgcc gccatcggcg
     1681 ccgccatcaa ctcgccggcg ccgccggcgt ggtccaggtg gacgtgggtg acgaacaccc
     1741 agtcgacctg cgcgggctcg aggcccttcg cttcgagcgc ggccagcaac agcggcacgg
     1801 aacggctggt gccggtatcc acgaacgcgg cgcggtcgcc gtgcaccacc aggtggctgg
     1861 cgtccaggcc cgggcgcacg tactcggtat ccacggcagt gatgccggcg tcgaactcga
     1921 tcaggctcgg cttgtgcaat gcattctctc ccgtatcgct ggcgccgatt gtgtcgcctc
     1981 gcggcgtaat gagcaaaaac gccggcgcga ggggccggcg ttcggagagt ctgccacggc
     2041 gtcggaggcc ggggcgggcg ggcggcgcta gcgcttcttc ggcaccgtca gccccggcag
     2101 cgaggcgtag tacgccgcca ggtcggcgat gtcggcgtcc gagaggccgg cggccagcgg
     2161 cgccatcagc gcattgctgc gctcgccgct gcggtactgc ttgagcgact tggcgaggta
     2221 gtcggcgtac tggccggcca gcaccgggaa gttcggcgcg atgccgcggc cgtcggcgcc
     2281 gtggcaggcg gcgcagggcg cggacttggc gcgaccgagt tccgggttgc cggcctgcgc
     2341 agtgccggcg agcagcagtc ccgcggcgag cgcggccagg ccgcgaaggt atgacgtcct
     2401 gctcattgcc cctgctcctc gaaccacttg gcgatcgcgg cgatctgctc ctcgctcagc
     2461 gtcgtggcat tggcgtgcat ggtggaatgc ggccgctcgc cgctggcgta ggcgcgcagc
     2521 gcatcgctga tgtactgcgc atgctgcccg ccgagtttcg gcacgtggta ggtcggatag
     2581 gcgttcttgt agcccgggat ggcgtggcag ccgaggcagg tttccacggc gtccggccgg
     2641 gaatcggccg ccgccggcgt cgcggccagc aggccggcca gggccagtcc gaacagtacg
     2701 gcgcgcgatt gcatcggcaa gcatcctccg ggtcgtcgcg gtggcgggaa atcgcggcgg
     2761 tatggtaatc agggaaaacc gaaaaagcca attccacggg gcgcgcggaa ccgcgcggcc
     2821 ccgcggccat cgaagccagt gacgctcgtc cgaccacctg cgcggagtac tttcatgctg
     2881 cacctccgga ttcttcttct cgccctgctg ctcggcctgc cggcggcggc cctggcctcg
     2941 gccgacgaaa cgcggcccac ggtgactgct tccggcgagg gccgggtgga agtggcgccc
     3001 gacatcgcct ggctcaattt cggcatcagc gcccgccagc cgacggtcgc ggctgcccgc
     3061 gacgaggtag cgcgcggcgt cgcccggctg atcgagctgg cccgccgcca ggggctgaag
     3121 gacgagcaca tcgccaccgc cgcgctgagc gtaagccccg agttcgactg gcaccccgag
     3181 acccgcgagc gccgcctgct gggctacgtg gtcagtcgcc aggtcacgct gcggctgacc
     3241 gacctggcga aactcggcga gctcaccgag caggcgctcg gcctcggcgt gaccgacgcc
     3301 tcacccgcca gcttcgacac gtcgcggcgc gcggaactcg aagccgaggc gctggccgcg
     3361 gcggcgcgcg acgcgcgctc gcgtgccgaa gtgatggcca gggcgctggg tgtggcggtc
     3421 ggcgcaccgc tgcggatcga ggccggcagc agcggcgtgt cgccacggcc gatgatgcgc
     3481 gcggccatgg aggcggacgc gatgagtggt gccgagacct accagccggg gctgatccgc
     3541 gtgaccgcca gcatctcggc gacctttctg ctgctcaatc cctgacgcct ggacggaagt
     3601 cgccgggcgg gcgaccgccc ggccgccgtc agtccgggtc gcgcacgatc cggacgaagc
     3661 ggctcggatg gatgttcagc ggattgtcct cgatgccgtg cacgtgcggc ttgaccagct
     3721 tcttgctgac gtagaaatac actggcagca gcggcgccgc ctcgagcgcg cgggcctcgg
     3781 cccgggcgag caaccgcagt cgcgccgcgc gatcgctctg tgacgccgcc tcccgcagca
     3841 ggcggtcgta taccgggtct gcccagccgg tgccattcac cgggctgtcg ccggccagga
     3901 tgccgaggaa gctctcgggt tcggcgaagt cggcaatcca gctgccgcgg tagacctgcg
     3961 tgacctcgcc ggcccgcacg ttggccagca gcacgcggaa ttcctcgcgc accaggctcg
     4021 tctcgacgcc cagcgcttcc ttccacatcg agcttaccgc cacggcgatc cggtcatgca
     4081 gctcgccggt gttgtagcgg atctccagcc gcagcggctg ctccgggccg tagccggccg
     4141 cggcgaacag cgcctgggcg cgttcgaccc gctcgcggcg gcgcgccgcg cactcggcgt
     4201 cgcgcggcgg tgcatagcca gggatgccgg gcggcaccag cgagcaagcc gggacttcgc
     4261 cggcgccgac gatgcgcgcg gcgatcagct cccgatccac taccagcgac agcgcgcggc
     4321 gcagccgggc gtcgtccagc ggcggccggc gcgtgttgaa accgaggaag tacaccgcca
     4381 ggtaggggct gacccggaat tcctccggca tgttctccac gagccagggg atgcgcgaca
     4441 ccggcaccgt gtagctcatg tccagctcgc cggcccggta gcgggacagc tcgctcgcca
     4501 ggtcggcgat cggcaggtaa cgcactcggt cgaccgccag cgaggcggcc gcgcgatact
     4561 cggggttgcg cgccagcagc aggtgcgagc cgggcacctg ttcgacaaag cggaaggcgc
     4621 cgctggacac ccggccgtcg gccggcaggt cgtcgcggcg cggcgacgcg gccgggtgcg
     4681 cgagcagttc gggcagccag ggcgtcggcc gcagcaggcg gatgcgcaag cggtccggcg
     4741 cgtccgccgt gacgccgacc tccgccacgg cggatcgtcc ggccgccgcc tcggcgaggc
     4801 cggcgaccgc gtcgaacagc ccgacgtgcg gcgaggccgt gacggggtcg gccagccgct
     4861 ggaaggcggt gacgaagtcg ctcgccgtca ccgggcggcc gtccgaccag cgcgccgccg
     4921 gctcgagctc gaagcgccac tccaggccgt ccgcggaggt ctcccacgcg cgcgccgcgc
     4981 cgggcacgac ctgcgccgag gcatccagcg tggtgagccc ctcgtgcagg tcgcgcagga
     5041 tgttcagcgc cggctcgctg cgcgcctgca gcgggtcgag cgactcgggg tcggcgttgt
     5101 tgccgcgcac gaactcgacc gcggcgctgc gcgcgcccgg cagcgacggc gcgtccgggc
     5161 gcagcaccag ccagcccagc aggaatgcgg cgcccagcac cgccagcagc cagcccgccg
     5221 ccgcgcggga catctcagta gcctcgctgc gccgtgtcga tcgacaggtg acgggtgtag
     5281 gtccggccca gcacgttgcc gctgaagccg cgcacccagg gtttcaccag cgccttgttc
     5341 accgggtggt agagcggcag cacgggatag tgttccagca tgcgccgctc ggccgtctcc
     5401 agcaggcgcc ggcgcgcggc ggggtcgagc tccgcggccg cggccgccag cagccgctcg
     5461 aactcggggt cgtcgtagcc ggtgtcgttc accgcgccat ggcgggagat gatctcgagg
     5521 aaattggagg cgtcgttgta gtcgccgcgc cacgacaggc gcaggacctg ccactggtcg
     5581 gggtgcttgc gccggctcag cagcacgcgg aattcctcgt tgtgcagcac ggtctcgaca
     5641 ccgaggtgtt cgcgccacat cgaggccacc gccacggcga tgcggcgcag gttctcgtcg
     5701 gtgttgtagt gcacctcgac cctgagcggc cgggccgggc cgtaaccggc tgcggcgaga
     5761 agctcgcgcg ccgccgccgc ccgtgccgcg atcggctggt catgccactc gtggcgctgc
     5821 gccgtgtagt tggcggtgcc cggcggcacc agcccgtagg cggcgtggtc gcccagcccg
     5881 gtgaccgtgt cgacgatgcg ctgccggtcc accgtcatgc tcagggcctg gcgtacacgc
     5941 agctcgtcgg tcggcgcgcg taccgtgttg aacagccaga actgcgtgga gatggcgggc
     6001 gcagtgtgca actcgtcggg caggttgtcg cgcagccact ggaaccgcgc catgggcatc
     6061 tgcatgctga aatcgagatc ccccgcgcgg tagcggttca gcgcggcctc gggcgcgtcg
     6121 aaagcgtagt ggcgcaccac gccgatggcc ggcggttcgc ggaaatgcgg gttggcgagc
     6181 agctgcacat ggctgtgcac ctcccaggcg tccagccgat aggcgccatt ggagaccagc
     6241 cggcccggac gcgcgaagct cgcgccatgc tcggcgaggc tggggcggtg caccgggaag
     6301 gtgatgggat tggtgagcag gccgagcagg tacggtgtgg ggctctgcag gcgcagctgc
     6361 aggcggcgcg cgtcgagcgc cgcgacgccg aggcgctccg gcggcagccg gcccgcgatg
     6421 atctccgttg cgctctcgag cggagccagc agcgccgcgc tcgagctggc ggtggccgga
     6481 tcgaccgcgc gccgcagccc cgccacgaaa tcctcggcca gcagcgggtc gccgttcgac
     6541 cagcgcccgt ccgcccgcag cacgaaggtc cagctcaggc cgtccggcga gacctgccag
     6601 gattccgccg cgccgggcac cagctcgccc gccggcgttt ctcccaccag cccctcgaag
     6661 aggtcacgga tgatctcccg gctgctctcg tcctcggccc gctgcggatc gagcgtgtcc
     6721 ggctccgggc cgttggcgcg ccggaacacc accggcgccg cggcggcctg cgccgccggc
     6781 tccgggccgg cgccctgccc gcagcccgcc gccagcatgg cggccaggca gggagctgcc
     6841 aaccatgccc cgctgcgcgg tgtgctcatt gtgggccggc ctctgcgacc gcgatgtaga
     6901 aatgccggct cggatgcagg tccatcgggt tcggctccca gccgcgcacc tcgggcttga
     6961 ccaggcggcg gcgcacgtcg aagtacagcg gaatgatcgg ctgctcgtcc agcaacagct
     7021 gctcggcctc gccgagcaac gccagccggc gcctcgagtt acgcgcctgc atggacgcct
     7081 gcagcaactc gtcgtattgc acgttgcgcc agccggtgtc actgccgccc gtctccgagt
     7141 cgaacaggcc ggcaaagctg tacggatcgc ggtagtccgc cgcccagccg gagcgaaaca
     7201 ccatggtggt gccgagatcg ttgcgcgaca cgaggaactc gccgaacggc tcttcacgca
     7261 ggcgcgtctg gatgcccagc gtctcgcgcc acatggcagc gacggcgatc gccagccgtc
     7321 gattgttcag cccgccggga tacagcagct cgagctccag cggccgcgcc gcatcgaatc
     7381 cggccgcctc gaacaggcgg cgcgcctcct caacctggcg gcgccgcggc aggctggccc
     7441 aggtgggcgt ctgtgctgca tagcctgcca tcggcggcac ccagctgtag gccggcacct
     7501 cacccgcgcc ggtcaccgag cgcacgatca gctcgcggtc gatcgccagc gccagcgcgc
     7561 ggcgcagccc tggggaaccg gcgaacggcg gccgcgtcac gttgaggccc agccaggtga
     7621 cggcgaggcg cggcgtgatg tgcagctcgt caccgagtcg gcggcgcacc agcccgagct
     7681 ggtggtgcgg cacgtcggag gtccagtgca gcttgccgtc gcggtagcgc tcaagttcga
     7741 cgtcctggtc ctcgatgggg tagtagatga cctggtcgat ggcgaccctg gggcggtccc
     7801 agtagttgtg attgcgcgcc agcgtgatgc tgtgccgggg ctgccagtcc gccaggtaga
     7861 acggcccgtt gctgaccagg cggccggcgc gggcgaagtc ctcgccgtgc tgctcgagcc
     7921 ctggacgata gagcggcgag ctgaccggat gggtcaggag atcgagcagc cacggcgtgg
     7981 gcgccaccag gtggatcacc agtgtgaggt cgtcgggtgc ctcgacgccg agttcctccg
     8041 gcgccatgcg ccgggtcatt accgcccgag cgttgagaat cggcgccagc aggccggccg
     8101 agccggaggc cgtggcgggg tccagggcgc ggcggatgcc ggccacgaag tcgcgtgccg
     8161 tgacgggctc gccgttcgac cagcgcgcat cggggcgcag cgcgaactgc cagcgcaagc
     8221 cgtcggcgct ggtctcccag ctggctgcga ctcccggcac gggcgtcccg tccggggccg
     8281 tgccgaccag cccttcgaac aggtcgcgca ggatgttcag cgaggacagg gacaccgcgc
     8341 gatgcggatc gagcgtcagc ggctcgctgc cgttgccgcg cttgagcacc tggaccccgg
     8401 gcttgagcac taccccgtcc gcgctctcct gcggctgccc gcaggccgcg accgcgagca
     8461 acagcgcgag ccacagggtc ctggcgacgc agcctggccg tccggcggtt gccacggcct
     8521 caggccactt cgccgtcggc acggccccgc cgcttcaggt gcaccagcag cagcgacacg
     8581 gcggccggcg tgacgcccgg cagccgcgct gcctggccga gcgtcgccgg ccgcgccgcg
     8641 tcctcagcca tcgcgcccgg catcggccag cagtctcgac tggtggtcgg cgatcagcgc
     8701 atcgatgatc tcgtccnnnn nnnnnnccgc gcgcagcttc tggcgcacct cgtgcgacag
     8761 gccgggcacc tgttgatagt cgatatcgtc cggcagccgg gtcgtctcct ggcggcggtg
     8821 gcgttcgacc tccaggtgct ggcggcgaat gtagccgtcg tagcggcagc gcacctcgat
     8881 ctgcgcggcg gtgtcgggtg cgacgcccgc atcgccgacg cagccgagcg ccaccaggtc
     8941 cgcgtggccg acctccggcc ggcgcaacaa ttccagcgcc ggcgcctcgc gggtcggcac
     9001 gccgcctgga aaggcggcgg cttcggccgg cgtgagcgcc gccggtcgca ccatgcaggc
     9061 ggccagccgc cgggtctcgc cttcgatttc cgcctggcgc cgctcgaagt cccgccagcg
     9121 ccgctcatcc accaggccga gctcgcggcc acgcggcgtc agccgctcgt cggcgttgtc
     9181 ctcgcgcagc agcaggcggt actcggcgcg gctggtgaac atgcggtagg gctccgcggt
     9241 gccgcgcgtc accaggtcgt ccaccagcac cccgaggtac gcctcgtgcc ggcccggcac
     9301 ccagggcgcc tcgccgcgca ggccgagcac cgcgttcacg ccggccagca gtccctgcgc
     9361 cgcggcttcc tcgtagccgg tcgtgccgtt gatctgtccc gcgaaataca agccgcggaa
     9421 gtggcgggtc tccagatgcg ggcgcaggtc gcgggggtcg aagtagtcgt actcgatggc
     9481 gtagccgggg cgcgtgagat gtgcccgctc gaagccggga atgctgcgca ccagctccag
     9541 ctgcacgtcg taaggcaggc tggtcgagat gccgttggga tagacctcga ggctgtcgag
     9601 gccttccggc tcgacgaaca cctggtgaga ggtcttgtcg gcgaagcgcg tgaccttgtc
     9661 ttccaccgac gggcagtagc gcggacccac cccctcgatg gtgccgctgt acatcggcga
     9721 gcgatgcagg gcggcgcgga tgatgtcgtg cgtgcgctcg gtggtggcgg tgatccagca
     9781 gggcacctgg cgcggatggt cctcgcgccg gccgaggaaa ctgaacaccg gccgcggctc
     9841 gtcgcccggc tgctcgcgca gctggccgta gtcgaggctg cgcgcgtnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gagccgattc gacggcgggt
     9961 cgccggcgcg gccgcccgca tggctggact cgccgacgtg gatcctgccg ccgaggaagg
    10021 tgccgacggt cagcaccact gcgcgggcgc ggaacgccag ccccagcgcg gtgagcaccc
    10081 cggccacgcg atcgccgtcc atcacgaggt cggccacgct ctgctggaat agctgcagat
    10141 tcggctgtga ttcgaccatg cggcggattg cggtgcggta cagcgcccgg tccgcctgcg
    10201 cgcgggtggc gcgcaccgcg gggcccttgc gggcgttcaa cgtgcggaag tggatgccgg
    10261 ccgcatccgc ggcgcgcgcc atggcgccgc cgagcgcgtc gatctcgcgc gccaggtggc
    10321 ccttgccgat gccgccgatg gccgggttgc agctcatctg gccgatggtc tcgatgttct
    10381 gcgtcagcag cagggtgcgc gcgcccatcc gcgccgcggc cagcgcggcc tcggtgccgg
    10441 cgtgcccgcc gccgaccaca atgacatcga attgctcggg gtgttgcatg gaatcaccac
    10501 gtggagcgct attttcgctc caccatctcc aggaactcgg ggtcatcacg cagcgccgcc
    10561 aggtcggtgt cgtgcttgat ccagccgaag ttctcgaccc ccgccgtgat cgcctcgcgc
    10621 agcgtgtcga gcgcgcgccg tttttcgccg agctgtgcgt aaaggcaagc gccgttgtac
    10681 agcatcaccg agtcgccggg gctcatctcc agcgctgcgg ccccctcccg aatagcgtcc
    10741 tcgcgacgat cgacctcgga cagcatgatt gcgtagaaca tgcgcgcacg cgagtcatcc
    10801 ggattctgca acaaatagtt cggcaacagc tcttccgcca gcttgtgcgc aagctcgagc
    10861 gactccttca cctgaccaag cgcctggcat gccatccgca gatcggcgtg ggcgacgtag
    10921 aaccgcggct tgagctcgat tacccggcgg aacagatcgc ccgcctcctg ggtcttgccg
    10981 gtcgagaaat ggattctgcc cagtgtccag taggcgatga agtcgtccgg atcgagttcg
    11041 atggccttgc ggccggactc cgccgcctcg tcgaacttgc cccagatgaa ataggacaag
    11101 cccatcgccg tgtaggcttc cggcagattg ttgtcgtaca tcagcgcctt gaaactcatc
    11161 tcctgggcct tgtcccgata gatctccttg cgcgaaaaat actggtacat ctgcccatag
    11221 gcgctggaac atgccgcgta ggccgcggcg tagcggggat cgagctcgat ggccttctcg
    11281 aacagctgga tcgcatattc cacgctgcgc ttggtcaggc ggtaaagata gtcctggccg
    11341 cgcaggtaga aatcataggc ctgtgcgttg accgtctggc gcttggtcag ggaaagcttt
    11401 tccgagaagc tgagctgcag cttcagcgcc tcgacgatct gcttggccac ctgctcctgg
    11461 atgtcgaaga tgtcgtcgag cttgcccttg taggtgttgc cccacagctg gcggttggtc
    11521 tcgacgtcca ccagctgcac cgtgacccgg accgaatcac ggaacctgcg cacgctgccg
    11581 ccgacgatgt agcgcgcgcc gagctcgctg ccgatcacgc gaatgtcctg cttcacgccc
    11641 ttgtactgca tcgaggccca acgcgagatc aggtcaattt cgctcaccag cgacaggcgc
    11701 gcgatgagct cctcggtgag gccgtcgctg aaatagtccg tttcctcgtc agggctgatg
    11761 ttgtcgaagg gaagcacggc aagcgcgccc ggctcgatcg actgttcggc agtgacgggc
    11821 gcctccgccg ccttgatgga gcgaagcttc gccccggcgt ccggcgccgc aggcttggcg
    11881 ggcgccttga gttcgacgcc gatgcgctcc agcgagcgca ggatggtctt caggttctcc
    11941 tcggggtcgc cctggaagta ctcgatgtgc tggatgcccg cgagcgggta acgaaaactc
    12001 ggcgggatcc gggtgggctc caggtggacc ggcaggatgt ggcccttgcg ctcgctggcg
    12061 atcacgactt ccttggcgac gttgtgcgac cgcaccgcgg actccgtcac catgagtaac
    12121 aggaccttgg ccttttccag cgcgtggacg atctcttccc cccacagcgt ggcgccgtcg
    12181 atgccgccga cgtccatcca gatcggcacg cccgcagcgc tgagcttcga cgtgagctca
    12241 aggaccttgt ccttgtcctc ccgcgagtag ctgatgaaca cgtccgccga ctgtgccaat
    12301 ggcttgcctc gttcatctgg cgggaggcag gaggagcgtc cgctcacctt gcctcaggcc
    12361 cgccgggact cacaaggcta gcctatcgcc ggggtactga gaaggagggc tgctgcaagg
    12421 caccaaaatg cgcattgctc gtttacgccg actcgtcgta gccggcggcg gcctcgttat
    12481 gagattcgag tcacagcgac cggcgcccgg ctgacgcatc ctgccgcctg cgggccgcgg
    12541 gtgcccggcc ggcaagagga gatcactcat tgggcacacc gttgcaggac aggcggcgcg
    12601 ctgaacccgc cgcggcgatg gatgcccgcc aggccgtgcg gctccgtcgc ttctacatgg
    12661 cgctggcctc ctatgccctc tgggccacca tcgcgctgat cgcctgggcc ggcaacctga
    12721 tcgtcatgcc tctctggctg ctcggcgcca ccctcgccgg ggtcgtgctg accaacgcct
    12781 ggttctggtt ccagctgcgc accggccgca gcttgctcta cgaagatccc tccatgacgc
    12841 tgcagcaggt ggcgctggcg ctgttttggc tgctggtcat catcggcgcc tcgcaggctg
    12901 accgcagtct catgatggtg gtctacatcg tcgtcatgct gttcggcatt ttccggctcg
    12961 agcagcgcca cttcctggtg ctcaccggaa tcgcgctggt cggttacgcc gtcgtggtat
    13021 cggccgacat cctgctgcga ggcgttgccg tcagcctgtt cgaggagctg gtgcgcttcc
    13081 tggtgctggc ctcctgcctg ctgtggtgca gcttcttcgg cacccacgtc gcccagctgc
    13141 gcgcccggct gcggcagcag aacgaggagc tgcagcgcca cgtgcagcaa gtgcagcgca
    13201 tcatggaccg cgatcacctg acctgggcct acaaccggca ctacatcatg aaccggctcg
    13261 acgaggagtg cgcccgctcc gagcatcgca acgcgccctt cgcgatcgtg attttcgacc
    13321 tcgatcactt caagagcatc aacgaccgct acggacacat cgcgggcgac cgcgtgctga
    13381 agaaattctc ggaggtcgca cggcgcgagt tgcgcgcggc ggacgtcatc tcgcctggcc
    13441 ggcggtccaa ctctttcggg cgcttcggcg gcgaggagtt cattgccgtg ctgcccgaga
    13501 ccggcctcga cggtgcgcgg ctgtgcgccc gccggctgca gacggcgctg agcaccgagc
    13561 agttcgagtc cgacatccgc gtgacttttt ccgccggcat cgccatccac cgccacgggg
    13621 agtcggtcga gcagactctg cggcgcgcgg acgacgcgct gtaccgcgcc aagcgcgccg
    13681 gtcgcgacca gatcctcgag gagaagccgg cccgcaagaa gccgcgcgcg ccgaacatcg
    13741 tccccctcgc ctcgaagggc ggctgagccg cacttacttg ccgatgcaga actcgctgaa
    13801 gatcctgccc agcaggtctt cggtggtgaa ctcgccggtg atttcgccga gcgccgcctg
    13861 cgcgagccgc agctcctcgg cggccagttc cacggcctgg ccccccgcca gcagcgcccc
    13921 ggcctgccgc agccgtgccc gggcgcgatc cagcgcatca aggtggcggc ggcgcgccgc
    13981 gaaactgccc tcgaccccgt cgccatagcc ggcgaacgcc ttcaggcgag cgcgcagcag
    14041 ttctatgccg gcgccggtca gcgcggacag gcgcagcacc tcgttaccgt ccgcatcccg
    14101 tccctggccc ggcggcagcc cggccaggtc gatcttgttc caggcgcgca gcaccggcac
    14161 gtcggcgggc agcgccggcg cactcgccgg cgccgtgccc ggcgcagcgg ccgcgctgca
    14221 gtcttgcacc agcagcacca ggtcggcgcg gtgcatcgcg tcgtgcgcgc ggcggatgcc
    14281 ctccgcctcg acccggtccg ccgcgtcgcg cagtccggcc gtgtcgacga cgcgcaccgg
    14341 catgccgtcg atcaggatgt cttcgcgcac caggtcgcgc gtggtgcccg gcaactcggt
    14401 gacgatggcc gcctcgtagc cggccagccg gttcagcagg ctcgacttgc cggcattcgg
    14461 cgcgccggcg atcaccagcg tcagtccgtc acgcagcacc cgaccgcgtt ccgccgccgc
    14521 gcgcaacgtg gcgaggcggc cgagcaggtc ctgcaactcg ccggcgagcg ccgggtccgt
    14581 gaatgcgtcg acgccttcct ccgggaaatc gagaccggct tcgaggcgca ctcgcaacag
    14641 ggtcagtgcc tcgaccagcg cgtgcaccgc gacggaaaac tcgccctcga gcgagcgcaa
    14701 cgcggcgcgc gccgccgccg tcgaggcgct ggccaccagg tctgcgacgg cctccgcctg
    14761 cgccaggtcg agccgcccgt tcatgaacgc ccgccgggtg aactcgccgg gaccggcctc
    14821 gcgcgcgccc agcgagacga gtcgctgcag cagcatggcg gcgatcaccg ggctgccgtg
    14881 cagctggaac tcgatgacgt cctcgccggt gaacgagtgc gggccgggga acgtcagcag
    14941 cagtgcgcgg tcgatgattt cgccgtcggc gtcgcgcagc acgcgcagcg cggcctgccg
    15001 ggcgggcggc aggctgcccg ccacggtggc ggccagttcc gtggcggacg gcccggacag
    15061 ccggagcacg gcgaccccgc cgcggccggg cgcggtcgcg atggccgcaa tggtgtcgct
    15121 gtcggcagaa gcgctcatca cgcctgcgtc aggcggcgcc gcgctccacg accttgttga
    15181 tgtgccactg ctgcgtgatg gccaacaggt tgttgacgaa ccagtacagc accaggccgg
    15241 ccgggaagaa ggcgaagaac accgtgaaga ccaccggcag gaactgcatg atcttcgcct
    15301 gcaccgggtc gggcggcgcg gggttcagct tctgctggat ccacatggtc acgcccatca
    15361 gcagtggcag aatgaacagg gggtcgcgca ccgacaggtc ggtgatccac agcatgaacg
    15421 gcgcctggcg catctcgacg ctctcgagca gcacccagta gagcgccagg aacaccggca
    15481 tctgcaccag gatcggcagg cagccagcgg ccgggttcac cttttccttc ttgtaaagct
    15541 ccatcatcgc ctgcgacagt ttctgccggt cgtcggcgta gcgctcctgc agcgccttca
    15601 tgcgcggctg cagcttgcgc atcttggcca tcgacttgcc gctggtttcc gacagcttgt
    15661 agaacaccgc cttgatcagc acggtcatca gcacgatggc ccagccccag ttcccgacca
    15721 ggccgtagat tttcgacagc acccagaaca acggctggga aatcaccgtc agccagccgt
    15781 agtcgaccgt caggcgcagg cggggcgccg tggcctgcag ctggtcctgc agcttcggcc
    15841 cgacgaacag ctgatcagtg aagatgctgg tctgcccggg cgcgacgacg gcgggcatgc
    15901 cgacggcgct cagcgtgtac aggccgtcgg tgttcgccga cgcggtgaac tgggcgcggc
    15961 ctgcgggcac cgcagcggcc aggaaatggt gctggatgga ggcgatccag ccgccctcgg
    16021 tggtgaactc gacgcgtgac tttgccaggt cgtcgatgtt gcgcttctcg tacttctcgc
    16081 cgttgaaggt gactggcccc cggaacgagt agctttccac gttcatcatg ctgcgattcg
    16141 gcggattgtg cagccggcgg atctgcaggt agctcgcacc ggtccacggg gcctcgccct
    16201 cgttggcaac ctgctgctcg agctggacgg cgtagctgcc ccgcgtgaag cgataggtct
    16261 tggtgaccgt cacgccgttg tcgaggcgcg cggtgagcgg cacttcgatc gcgtcctggc
    16321 cgggcgccag ccggtattcc atggccggcg cgacgaactc ggtgtccggt cgcgtggaag
    16381 tctcggccgc ggtggatgtc aggccggcac gaaagacgaa caggccgtcg cgtgtcgggt
    16441 cgagcagctg caccggcacg tctggctggt tcttgtgcac cgggtaggtc ggcagcagcg
    16501 ccttgacgag gtcgccgccg cgcaacgaga tctccagctc cagcacgtcg gtggccacgc
    16561 ggacccggcg ctcctcggct gcgggcgcag ccgcatcatc ggccgccgcg gccacggcgc
    16621 cgcccggtgg caccgggatt tcgttgcccg acggcagtgc ggggaccgtt gcttcctccg
    16681 gcaatgccgg ggccaggcgc tcatcgaccg gcggcggcgg ggcggccggc cgctggtact
    16741 cgatgaaggc catgtagttc agccagacca ggagcgccag ggcgatccag agaaatactc
    16801 gctggttgtc catgcgtttt gcgcgactcc tcaggcgatc ggccgacgcg gcggcacggg
    16861 gtcgtgtcca tggccgcccc aggggtgaca acgtccgatg cggcgcgacg ccagccacag
    16921 gccgcgcacg acgccgtgcg cctgcaccgc ctcgatggcg tattgcgagc aggtcggctg
    16981 gtagcggcag ttgttgccaa gccacgggct cagcagcagc tggtagccgc ggatcaggaa
    17041 gatcgcgatg cttcgcatag gcgtgccgtc cgttcgagca gggcgtcgat gctgctggcg
    17101 aggcgggaat tatcgcatcc ggcggctccc ggtcgcgcca tcacgacgat atcgagggcc
    17161 ggcaggtctg cgctgtgctt gcggaaggat tctctcacga cgcgcttgat ccggttacgc
    17221 tgtaccgcgg acttggacac cttcttcgag accgcgaggc cgagcctggc tgccgatccg
    17281 gaaccgggcg ccgcgatgac gttgaagcag tcgttgccgc tgcgtcggcc ggtcctgaac
    17341 acgcgcgcga actgggccgg ggccaacagg cgctgcgcgc gagtaaagga ctcggatcgt
    17401 gtcgtgggtg ccggggctgt cctgacggcc gtggctgccg agcgcgcgtc agacggcgag
    17461 cctggcgcgg cccttggcgc gccggcgcga cagcacctgg cggccgttct tggttgccat
    17521 gcgggcgcgg aatccatggg tccgcttgcg gcgcaggttg ctcggttgat aggtgcgttt
    17581 catcgtactg attccgtccg agtgatgccg gcgacagacc cgacctgggc gcctgccaaa
    17641 aagacgggaa atcgtacgcg cggccccctg gagtgtcaac ttggccaacg cggtacaaga
    17701 ccggcgcgcg ggctgtggat aactcggccg gggcaggcta gactgccgac cttctcccgg
    17761 gatccctcga gcgcaggacg caaagtggcg gccgtctggc accaatgtct cagcaagctt
    17821 cgcagtgaac tgtctccgga ccagttcaac acctacgtgg cgccgctcca ggcggtccgc
    17881 gagggagaag ccctgcgcct gctcgccccg aacccctacg tcgtgtcgtg gctgcgccag
    17941 aattgcctgg cgcggatcag ggaactgacc gccgacgagg cgagcggcag gggcccggtc
    18001 gaggtatcga tcgatgtcgg cacaagggcc ccggacatgc cggcgggtgc cgccgcgcca
    18061 cgcgagtccg cggcgccgac gccggcgggc cccactgtgc cgcgcggcga attcgtcacc
    18121 ggcgcccgcc tgaacccgga tttcaccttc gagagcttcg tcgagggcaa gagcaaccag
    18181 ctggcacgcg ccggcgcgtt ccaggtcgcg gcgaaccccg gcggctcata caacccgctg
    18241 ttcctgtacg gcggcgtcgg cctcggcaag acgcacctca tgcatgccat cggcaacgcc
    18301 atcctgcagc gcaacccggg cgcaagggtc gcctacgtgc actccgagcg cttcgtcagc
    18361 gacatggtgc gggcgctgca gaacaaccgc atcgacgagt tcaagcgcag ctatcgctcg
    18421 ctgaacgcgc tgctgatcga cgacatccag tttttcgccg gcaaggaacg ctcacaggaa
    18481 gagttcttcc acactttcaa cgcgctgttc gaggaacgac agcagatcgt gctgtcgtgc
    18541 gaccggtatc cgaaggaagt cgacggcctc gaagatcggc tcaagacccg ctttggctgg
    18601 ggcctgacgg tcgccatcga ggcgcccgag ctcgaaacca gcgtcgcgat cctgatgaag
    18661 aaggccagtt tcgaaggagt aacgctgccg tccgaggtgg ccttcctgat cgccaagcga
    18721 attcgctcca acgtgcgtga gctggagggc gccttgcgcc gggtgatcgc cacgtccagg
    18781 ttcctcgacc gcgagatcac catggacttt gccaagaagg cgctcggcga cctgttgtcc
    18841 ctgcaggacc ggctggtgac catcgacaac atccagaaga ccgtcgccca gtactacaag
    18901 attcgcgtcg ccgacctgat gtcgaaacgg cgcaaccgtg ccgtggcgcg accgcggcag
    18961 atcgccatgt gcctggcgaa ggaattgacg gagcgcagct atccagagat cggcgatgcc
    19021 ttcggcggca aggaccatac cacggtgctg catgcctgca aacgcatcaa ggaactgcgc
    19081 gaaaccgaca tgcgggtccg tgaggattac gcaaacctgt tgagaacact gacggcatga
    19141 tgtggaaaag ttgtggataa actcctgggc caaaactgtc cacagggcat ccacagcatc
    19201 ccgaggccct tcgcaggcat gcgaccacag ggattaccaa tgcgcaagta tatgatttat
    19261 aaatactaat gcagtttatc cacagatgcg ttgcggacta ttagtaacaa caacagattt
    19321 ctatatatct actctcatta gttaacaggt ggcacgatga agttcagcgc tccgagagaa
    19381 gctctcctcg aacccctcca ggccgtcatc ggcgtggtgg aacgacgcca gaccatgccg
    19441 gtgctggcca acgtgctgtt gatcgcgggc gagaagacgg gtttgtcggt gacggccacg
    19501 gatctggagg tcgaattggt ggccacggtc gacgtacagg tcgagaaggg tggcgagatc
    19561 accgtgcccg gccgcaagct gctggatatc tgccgggcgc tgccggatgg cgctgcggtc
    19621 agtttcagcc tgtcgggcga caaggtcacg gtgcgctccg gtcgcagccg tttcacgctg
    19681 tccactttgc cggcgagcga atttcccacc gtcgaggaca ttcgcgccca gcaggcgatc
    19741 cgcctgccgc aaggccagct gcgacgcctg atcgagaaga cccagttttc catggccaac
    19801 caggacgtgc ggtattacct caacgggatg ttgctggaag tggccggccc gctgatccgc
    19861 agtgtcgcga ctgacggcca tcgcctggcg ctgtgcgagc tgtcgctgga caaggcggac
    19921 tcgagcccgc agcaggtcat cctgccgcgc aagggtgtgc tggagctgca gcgcttgatc
    19981 ggcgagggcg gcgattgcag cctggagatc ggcaccaatc acctgcgggt gacgctggac
    20041 ggcattcggc tgacctcgaa gctgatcgac ggccgtttcc cggactacga ccgggtgatt
    20101 cccaaggaaa gcgacgcgcc catgctggcg gaccgggtgg aactggcgga cggcctgaac
    20161 cggatcagga tcctggcgaa cgagaagtac aagggtgtgc gggtgacgct caaaccgggc
    20221 agcgtgaccg tacaggccca caatcccgag caggaggaag ccgaggagga aatcgaggtg
    20281 cagtacgacg gcgccgagat ggagatcggt ttcaacgtgg aatacctgat cgcggcactg
    20341 cgcgcgctgg acgacgacaa agtgcgactg gagttccggg atgccgccag cagctgcctg
    20401 atccaggatc cggagcgcag ggactgcaag tacgtcgtga tgccgatgcg gctgtagcgc
    20461 ctcgcatggg actgagaagc ctggcggtca cggggcttcg ctgtttcgag agtgcgacgc
    20521 tggagccggc acccggattg aatctcgtga ccggcatgaa cgcctcgggc aagaccagct
    20581 tgctcgaggc gatttttctg ctggggcggg gccggtcgtt ccgggccgca cggcgggagg
    20641 ccgtcatccg ggagggcgct gagcagctgc gggtggtggc ccggctggcg gacgggcgca
    20701 tgctgggaat tgaggccggg cggggctc
//
LOCUS       DAOMPG010000009        20638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1119_length_20638_cov_4.658310, whole genome shotgun sequence.
ACCESSION   DAOMPG010000009 DAOMPG010000000
VERSION     DAOMPG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 20638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1119_length_20638_cov_4.658310"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="gcvPB"
                     /locus_tag="PLS34_01005"
     CDS             <1..968
                     /gene="gcvPB"
                     /locus_tag="PLS34_01005"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRP86080.1"
                     /translation="HGTNPATATMCGCKVIEIPTGPDGDVDLAALKAAVGPNTAGIML
                     TNPSTVGVFDRKILEMSKVVHAAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHKT
                     FSTPHGGGGPGSGAVGVSERLVPFLPVPVAAKEGERFRWVAEDERPLSIGRLSAFMGN
                     AGVLLRAYVYARMLGREGMPRVAEFATLNANYLMARLRDAGFQLAYPERRASHEFIVT
                     LKRQAKDLGVTAMDFAKRLLDFEFHAPTTYFPLLVPECLLIEPTETESKEALDAFVAA
                     MAQILAEAHSEPEKVKGAPYTTPVRRLDDVRAARQLDVAWKPAAA"
     gene            965..1909
                     /locus_tag="PLS34_01010"
     CDS             965..1909
                     /locus_tag="PLS34_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRP86081.1"
                     /translation="MSALICGSIAYDNIMVFQGRFQDQILPDQIHVLNVSFLVPALRR
                     EFGGCAANIAYNLRLLGDVGYPMATVGHDFGPYRDWLRERGVPLDHVRQLGDSWTAVC
                     YITTDLADNQITAFHPGAMEHAHVNKIPADAGIRWGMVSPDGRQAMIDHAEQFAAAGI
                     PFVFDPGQGLPMFGARELERFLELADVVAVNDYEGKMLAQRTGLDEAEIAARVRAYIV
                     TRGAEGSVVHANGARLEIPAAAPDAVVDPTGCGDAYRAGLLHGLLHEADWQTTGRIAS
                     LMGAIKVESPGTQNHSFDRLSFSERFMASFGYDPGLGS"
     gene            1913..4732
                     /locus_tag="PLS34_01015"
     CDS             1913..4732
                     /locus_tag="PLS34_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRP86082.1"
                     /translation="MQRFAACIALALAPAFAGPVLADQAPLAVEGERSDWRATLERVA
                     PSIVTIRVDGTRAFDTNWNMSSQATGFVVDAERGLILTNRHVVTPGPVMAEAVFLNHE
                     EVELTPVYRDPVHDFGFFRYDPAKLRHIRPETLPLAPQGAQPGREIRVVGNDAGEQIS
                     ILAGTIARLDREAPFYGRGQYNDFNTFYIQAASSTSGGSSGSPVLDIQGRVVALNAGA
                     NVQSASSFFLPLGRVTRALERIIAGEPVTRGTLLTTFTHVPYDELGRLGLDAATEAQY
                     RQLFPRQKGLLVVRDLVPGAPAEGQLEPGDIVLAIAGKPLATFEALAEVLDEQVGERI
                     EIAVRRGSTDHQFVIEVTDLHAVSPAEFLQIGDAVLHDLSYQQARHLNMPLEGVYIAN
                     PGYMFMASAVPRGAVIVEINGQPTPDLDALRALIEGVPDRARIAVRYYDFDNPRATNL
                     RSVRVDRRWFPAAHCRRNDALGDWPCEALPEVGVAPLPPPATTSFPPESDRHLRTLQA
                     SLVMVNFEMPYPVSGVSDRFYHGTGLVVDAERGLVVVDRNTVPDSLGDITLTFAGSLE
                     TQARVAFVHPLHNLAVLQYEPRLIAATPVRSATLSDVPLKSGDEVRVVGLRGDHRLAS
                     QSAKVGSVDALNLPLSRTLRFRDTNLETVQLVGGPDEFDGVLADGRGRVLGLWSSFAY
                     ESGRGTEQANKGVPAALVAEVLALARGERALRSLEAEFLQLPLASARRLGLADEHAAM
                     LEAHDPERRQVLQVVRTVAATPAARMLRPGDLLLSINGATVNRFRAVEEASQVPVAQA
                     RVWRQGEVLDLEIPTVELGSGGVDRVVLWAGALLQPPHRALAAQRGIEPSGVFVAFHS
                     FGSPASRNGVGAGLRIVEVDGQPTPDLDSFLRAVEGRAHRAAVRLRTVNWNGQPGVYT
                     LKLDNRYWPPYELVRNEDGWTRRDL"
     gene            complement(4811..6883)
                     /locus_tag="PLS34_01020"
     CDS             complement(4811..6883)
                     /locus_tag="PLS34_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRP86083.1"
                     /translation="MPERRNRLADETSPYLLQHADNPVDWHPWDAQALELARREGKPI
                     LLSIGYSACHWCHVMAHECFEDEAIAAQMNELFVNIKVDREERPDLDRIYQLAHQMIT
                     QRNGGWPLTMFLSPDDQTPFFGGTYFPPTPRHGLPGFPELLERVSAYWRDHREEIRQH
                     AEGVREAFAAVWEAPTAEAGGTELDGEPLGRARRELGETFDREHGGFGGPPKFPHPPN
                     LQRLMRHWRTTALGESPDLEALYMATSTLQQMAEAGIYDQLGGGFCRYAVDAHWMIPH
                     FEKMLYDNAQLLPIYAQAWTVTNEELFRRIATGTAGWVMREMQAPEGGYWSSLDADSE
                     GEEGRFYAWTREELQRLLEPAEFAVVSGIHGLDRGPNFEGRWHLHTFMSADQFAAGSG
                     LDGAEVRMRLASAHAKLFAAREKRVRPGRDEKVLAAWNGLMIRGMAVAARILGDAALA
                     DSAARAMAFVRREMLVDGRLRATWKDGRARLAAYLDDYAFMLDAALEMLQLRWDTDDL
                     DFAVRLAEALLAHFEDPASGGFLFTADDHEQLIERPRPLADDALPSGNGVAALALNRL
                     GCLLGEPRYLESAERAVRAALPVIRRAPFAHCALLDALEEQLSPPEIVIVRGAPKRAA
                     DWARTARLVYAPRRLVFDVPATAMDLPAALAEKTVPESGARAWICRGTTCLPPVDSLA
                     GLTTTLKG"
     gene            6987..8156
                     /locus_tag="PLS34_01025"
     CDS             6987..8156
                     /locus_tag="PLS34_01025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRP86084.1"
                     /translation="MPELKLKPREDRRLRAGHLWVYSNEVDTAATPLSTLAPGAIVRL
                     SSAEGRFLGYAGVNPHSLIAARILSRDAAHPPDRSLLVHRLKVALSLREQLYEHGCYR
                     LLFADGVPGLIADRFGDVVVLQSGTAAMEAMKGEVVDAVRKVIAPAGILWRNDASGRE
                     FEGLERYVEVAHGQVPDQVTVNEAGVELTAPIRAGQKTGWFYDQRDNRTRFGRYRPAS
                     VLDVFSYTGAWGLSAAAHGARATLVDSSAAALELAAGDADRLGLEVEALQGQAFDVMK
                     ALADEGRNFAAVVVDPPAFIKRRKDHKAGLAAYQRANQLAMRLLGRDGLLVSCSCSFH
                     LSQDDLLAVVQRAARHLGRVVQVLEIGGQSADHPSHPLIPETRYLKAVFCRVLAG"
     gene            complement(8117..9223)
                     /locus_tag="PLS34_01030"
     CDS             complement(8117..9223)
                     /locus_tag="PLS34_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP86085.1"
                     /translation="MQENGPALQVAILAMPESTASTLYGMCDLLASAGRDWAALIEGR
                     SAESRINPWVVSVDGRRMALPNGLSVEPACAIADAPRPDVIAIPDLAVLPGEDLADRY
                     LAERDWIRTRYDDGVTLATACTGGLVLAEAGLLDGHDATVHWAYCDAMARRFPRVRVH
                     PNRALVITGHDQRIIMAGGGTAWHDLALYLVARHLGAEEAMRVARLHLIDWHHLGQQP
                     FASLSCKRQSDDAVISRCQEWVAQHYEEDAPVGAMVQRSGLSERSFKRRFQRATGMPP
                     LEYVHTLRIEEAKHLLETTDAPVEAIANEVGYADASFFGRLFRRKVAMTPAQYRRRFG
                     ALRRMLEGGAGAGAGSSAPAVIQPASTRQNTALR"
     gene            9334..10113
                     /locus_tag="PLS34_01035"
     CDS             9334..10113
                     /locus_tag="PLS34_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRP86086.1"
                     /translation="MPTPWEILLDPLSLTIIGLFAGLATWEALAPRRVLPAMPGWRLR
                     GLAAFAVFFFLSSYLPMLWDDHLAAWQLFDLSGLGVAGGALAGMLVSQLLGYWWHRAL
                     HRSDTLWLGLHQMHHSAERLDAWSAYWFSPLDMVGWTAVGSLALVLVVGVDPRAATLV
                     LLLTSFLDIFTHANVRTPRWLGYLVARPEMHAVHHTRGVHRYNFASLPVLDMLFGTYR
                     NPRTWDHQTGFYDGASRRVGAMLLFRDVSQPEGPPREPAEA"
     gene            10271..11053
                     /gene="lgt"
                     /locus_tag="PLS34_01040"
     CDS             10271..11053
                     /gene="lgt"
                     /locus_tag="PLS34_01040"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRP86087.1"
                     /translation="MIPYPNIDPVAIQLGPVAIRWYGLMYLIGFAIAWFLARHRAKQP
                     GSGWTPQQIDDLIFYSALGVIVGGRLGSILFYNFDAWLRDPVMLVRVWEGGMSFHGGL
                     LGVVLAMWLYGRKLGRGFFAMTDFVAPLAPLGLAFGRLGNFINGELWGKPSDVPWAFV
                     VDGVARHASQLYQAALEGVALFLILWFFSARRPPRMAVSGMFALGYGVFRFLVEFVRL
                     PDANIGYLAFGWLTMGQLLSVPLILLGAVLLWLAYRPAARAA"
     gene            11057..11890
                     /locus_tag="PLS34_01045"
     CDS             11057..11890
                     /locus_tag="PLS34_01045"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRP86088.1"
                     /translation="MQQYLELLRRVRGEGLQKGDRTGTGTLSVFGHQMRFDLGAGFPL
                     LTTKKLHIRSIVHELLWFLSGSTNIGYLKEHKVRIWDEWATGEGELGPVYGRQWRAWP
                     TADGGAIDQVAEAVDTIRRRPNSRRIIVIAWNPAELPDETVSPQDNVRAGRMALAPCH
                     CLIQFWVGDGRLSCQLYQRSCDVFLGVPFNIASYALLTHMVAQQCDLGVGDFVWTGGD
                     VHLYLNHLEQADLQLQRSPGPLPRLRILRRPPSIFDYRYEDFEVVGYEARPHIPAPVS
                     V"
     gene            complement(11927..12394)
                     /locus_tag="PLS34_01050"
     CDS             complement(11927..12394)
                     /locus_tag="PLS34_01050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRP86089.1"
                     /translation="MDESQSIQLTLELQQDYQFSIRFEGAELEPLLTDEPPPLGEGSG
                     PNPSQLLLAAIANCLSASLLFAMRKFRNEPARLRATVTAVRERNAAGRWRIPRAEVTI
                     HLPDAADDYRNLDRILAQFEEFCIVTQSVREGIEVTTRIEDANGLRLRALPAG"
     gene            12642..13145
                     /locus_tag="PLS34_01055"
     CDS             12642..13145
                     /locus_tag="PLS34_01055"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRP86090.1"
                     /translation="MPEISLVAAVADNGVIGADGGMPWHLPADLTHFKQLTMGKPVLM
                     GRLTWESIGKPLPGRLNLVLTRDPAWQADGAQRAASLDDALRAAQRSGAVELMVIGGA
                     DVYRQALPRAHRIYMTRVHAEPWGDTLFPELEPGDWEEVARRERLSDERNPWDLTFVV
                     LERVAGD"
     gene            13201..15036
                     /locus_tag="PLS34_01060"
     CDS             13201..15036
                     /locus_tag="PLS34_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86091.1"
                     /translation="MKNLLAELQRRNVTRVAMLYVVAAWLLLQVADVGISTLGLPEWS
                     GRLVLLLLALGLPVALVFAWVYELTPEGIRRESEVPPAESVVGQTAQKLNVAIIVLLV
                     IAIAAIALDRLLPGAPSPAEAGDTAETQAEPSSPGAAEAATVAAAPMQAPVTETSIAV
                     LPFIDMSPGRDNEYFSDGLTEELLNSLVKIRALQVAGRTSSFAYKGRDLDMRVIGDEL
                     GVAHLLEGSVRKAGNRLRITAQLIKVADGYHLWSETYDRELTDVFAIQSDIAEHVVEA
                     LQITLLGEDAARMRAGGTTDVEAFNDYLQGVYLANQGSREQSVRAAVAAFERALARDP
                     AFVDAWVALGATMSHMNANAWESPELAWARIEEAATEARRHAPQLAGGYMLESLLHGF
                     RDRRWMEAIEAMRTAVALEPGNANLLLAYSQLASQMGLHEESVRAAQRAVELEPTNLT
                     HQAYLGRGLQFAGRCPEAEVVLRRAIERDPRFPRPRYYAGICRYLAGDPEAALALFEA
                     EPLPWMRRAGRPLALHGLGRLDEAQAAWDELVAAHGDTAAFQKAEIAAQWGDNELVFL
                     SLERAFETRDAGLNLLLVSPLLAPLRDDPRYLQMVERMGMAPLLE"
     gene            15086..16846
                     /locus_tag="PLS34_01065"
     CDS             15086..16846
                     /locus_tag="PLS34_01065"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRP86092.1"
                     /translation="MTSETLQYGTMALGLFGGLALFLFGMEQMTVALKEAAGGRMKSL
                     LARMTKNRFRALAAGAFTTAVIQSSTVTTVLTVGFVQSGLMSLQQAIGIVMGASIGTT
                     VTAQIVAFKVTNLALLAIALGFLAHTAARSERLRRYGTMVLGLGLVFLGMNLMGEAMA
                     PLRDWPPFAAAMQQYSWPLAAVVIGALFTALVQSSSATTGVIIVMAGQGMIPLETGLA
                     LALGANLGTTITALLASIGKRAEAVQTALAYFIYKLVTSAAWLLLLPTLAAAMRVVSP
                     TFPELDGIERLGAETPRQIANGYLLLNMASAAVCIWFTGPLARLVERLHPGRPEPVPT
                     VEQPKYLDDALLDTPDLALDRVRMELGHLGETVLRLLGRGPAMVYEGRSETIDEIPRL
                     DGDVDSLHDAIISYLGRLSQRSMNAAQTELLHDYMAAAGLFESMGDIVEMNLSHAARI
                     RLAAGLEVSAATREVIGQLWDKVYWSVGQAARAVASSSEEAAHAVIESKDEVNRLVRA
                     AELHLARRLSASEAHRLSLFRIESDMVEAAKRIHYFARRAAKRMVEIDMPAEPDRGQG
                     EPPDPAGPVATGSGGAPPSA"
     gene            complement(16812..17756)
                     /locus_tag="PLS34_01070"
     CDS             complement(16812..17756)
                     /locus_tag="PLS34_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRP86093.1"
                     /translation="MLTRRQYLQLSLAAGAALAVPRPLLAAAERALEPITRKIPATGE
                     RVPAVGLGSSATFAAVARSEDVSALREVIGAMLEHGGTIFDTAPSYGASEQVAGAIAR
                     ELGLTEKVFWATKLNVAGRDGGRADPAAAKAQLEESFRRIGRETIDLIQVHNMADVPT
                     QLGLLKEYKEAGRVRYIGVTTTFPAQYAGLIEVMRSEPLDFIGIDYAIDNLAVEETIL
                     PLAQDRGIGVLVYAPFGRTRLWERVRGHEVPAWAAEFDAASWGQFFLKFVLAHPAVTA
                     ATPATSRARHMIDNMGAARGRLPDADMLKRMVAHLRSL"
     gene            complement(17763..19103)
                     /locus_tag="PLS34_01075"
     CDS             complement(17763..19103)
                     /locus_tag="PLS34_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP86094.1"
                     /translation="MIDPVARIFNVRREERIPVLAAAAFFFCVLTALMVLRPAREALG
                     MQRGIEAVRWLFMGTAVVTLLVNPVFGLLVSRFRRLQFISATYLFFAFSLVGFYLLLT
                     AAPAAVGVTSGQVFYVWFSVFNLFITMLFWALMADRFSLEQSKRFFPLIAVGGTSGAI
                     FGPWLAAMLVQSVGTPALLLVSTAFLVLALGAALAVAQTQPHRPDAAEPSTPEMVPVV
                     DEQAVIGGSAWEGVRAVLRSRYLTGIAAYALILAVISTFIYFTRLQMVAALGDDIDLR
                     TAWFARIDVITQAATLVLQALVTGRLMRRLGVPLTMALLPLTVALGFIGLALVASLAA
                     LITFEAAFRAVQRAIMRPTRETLYTVVSREEKYKAKAFIDTFIYRGGDVVGAQAEGLL
                     GRLGWGLVALASVAVPLAVAWAVLGMWLGRMQRRLAGGTEPVAPDRLERMEHGR"
     gene            19253..19681
                     /locus_tag="PLS34_01080"
     CDS             19253..19681
                     /locus_tag="PLS34_01080"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRP86095.1"
                     /translation="MGKIDMAMEGIRLVWKDSFNSGHALIDKQHRGLFELANELLDAM
                     HAAAPRDEVLKVVDRLLHDIARHFADEEELLERAGYLSLYEHSREHAALVGKARMLAD
                     AFRQGTLHIRELFQFLAYDVVTRHMLGTDRHFFPYINDQP"
     gene            complement(19672..>20638)
                     /locus_tag="PLS34_01085"
     CDS             complement(19672..>20638)
                     /locus_tag="PLS34_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRP86096.1"
                     /translation="GWRNWWLEAAWWDMAGKLAGKPVYKLLQDGEETVDRARVYASTG
                     SLRPLGERRPYLDEIRRMGIGAVKIRVKYPTLEEDLALVRGVREYLGDDFVIGVDANQ
                     GWPVSLFEPNPDWDLARATAFARGCDELGIAWLEEPLDMHDWHGMAELRRRVKVQLAG
                     GELHGAWHELLPLFEHDSLGKYQPDAMFSGLTVVRQVMAECRKRGLDFTPHTWSNGFN
                     MVVNLHAFAAWERRKLIEFPYEPPGFVPAAREGIMPPIEVRADGTIEVPQSPGLGLQI
                     DERLLRRHGRRFHVSTQRRVAVRAIREKGLKAALTLKKAKDAAQG"
BASE COUNT         3111 a         7028 c         7144 g         3355 t
ORIGIN      
        1 cgcacggcac caatcccgcg accgccacga tgtgcggctg caaggtgatc gagatcccca
       61 ccgggccgga cggcgacgtg gaccttgctg cgctgaaggc cgccgtgggc ccgaacaccg
      121 ccggcatcat gttgaccaac ccctcgaccg tgggcgtgtt cgaccgcaag atcctagaga
      181 tgtcgaaggt ggtgcacgcg gcgggcggcc tgctgtacta cgacggtgcc aacctgaacg
      241 ccattctcgg caaggtgcgc ccgggcgaca tgggcttcga cgtcatccac atgaacctgc
      301 acaagacctt ctccacgccg cacggcggcg gcggcccggg ctccggcgcg gtcggcgtga
      361 gcgagcggct ggtgccgttc ctgccggtgc cggtggcggc gaaggagggc gagcggttcc
      421 ggtgggtggc cgaggacgag cggccgctgt ccatcggtcg cctgtcggcg ttcatgggca
      481 acgccggcgt gctgctgcgg gcctacgtgt acgcgcgcat gctgggccgc gagggcatgc
      541 cgcgggtcgc cgagttcgcc acgctgaatg ccaactacct gatggcgcgg ctgcgggatg
      601 ccggttttca gctggcgtac ccggagcggc gcgcgagcca cgagttcatc gtcacgctca
      661 agcgccaggc gaaggacctg ggcgttacgg ccatggattt cgccaagcgg ctgctcgact
      721 tcgagttcca cgcgccgacg acctacttcc cgctgctggt acccgaatgc ctgttgatcg
      781 agccgacgga gaccgagtcg aaggaggcgc tggacgcctt cgtcgcggcg atggcgcaga
      841 tcctggcgga ggcccacagt gagccggaga aggtaaaggg cgcgccgtac accacgcccg
      901 tgcggcgcct cgacgacgtg cgcgcggcgc ggcagctgga cgtggcatgg aagccggcgg
      961 ccgcatgagc gccctgatct gcggctctat cgcctatgac aacatcatgg tcttccaggg
     1021 ccggttccag gaccagatcc tgcccgacca gatccatgtc ctgaacgtct ccttcctggt
     1081 gccggccttg cggcgggaat tcggcggctg tgcggccaac atcgcgtaca acctgcggct
     1141 gctgggcgat gtcggctatc ccatggccac cgtcggccac gacttcgggc cctaccgcga
     1201 ctggctccgc gagcgcggcg tgccgctcga ccacgtgcgt cagctgggcg actcctggac
     1261 cgcggtctgc tacatcacca cggaccttgc cgacaaccag atcacggctt tccacccggg
     1321 cgcaatggag cacgcgcacg tcaacaagat cccggccgac gccggcatcc gctggggcat
     1381 ggtgtcgccc gacggccgcc aggcgatgat cgatcacgcc gagcagttcg ccgcggccgg
     1441 catccccttc gtcttcgatc cgggccaggg actgccgatg ttcggcgccc gggagctcga
     1501 gcgcttcctc gagctggccg acgtggtcgc cgtgaacgac tacgagggca agatgctggc
     1561 ccagcggacc ggcctggacg aagcggagat cgccgcgcgg gtgcgcgcct acatcgtgac
     1621 gcgcggtgcg gagggctcgg tcgtccacgc gaatggcgcg cggctggaga tcccggcggc
     1681 ggccccggat gcggtggtcg accccaccgg ctgcggcgac gcttatcgcg ccggcctgtt
     1741 gcacggcctg ctgcacgagg cggactggca gaccacgggc cggatcgctt cgctgatggg
     1801 cgcgatcaag gtcgaatcgc ccggcaccca gaaccattcc ttcgatcggc tgtcattctc
     1861 cgagaggttc atggccagtt tcggttacga ccccggcctc gggagctgat cagtgcagag
     1921 attcgccgca tgcatcgcgc tggcgcttgc gccggccttc gccggcccgg tgcttgccga
     1981 ccaggcaccg ctggcggtcg agggggagcg ctcggactgg cgggccacgc tggagcgcgt
     2041 ggcgcccagc atcgtgacca tccgtgtcga cggcacgcgc gccttcgaca ccaactggaa
     2101 catgtccagc caggccaccg gcttcgtggt cgacgcggag cgcggcctga ttctcaccaa
     2161 tcgccacgtg gtaacgcccg gcccggtgat ggccgaggcg gtcttcctga accacgaaga
     2221 ggtggaattg acgccggtgt atcgcgaccc ggtgcacgat ttcggcttct ttcgatacga
     2281 tccggccaag ttgcgccaca tccgcccgga aacgctgccg ctggcaccgc aaggcgccca
     2341 gcccggccgt gaaatccgcg tggtcggcaa cgatgccggc gaacagatct ccattctcgc
     2401 cggcaccatc gcgcgcctgg accgtgaagc cccgttctac ggtcgcggcc agtacaacga
     2461 cttcaacacg ttttacatcc aggcggcctc cagcacgtcc ggcggttcct cgggctcacc
     2521 ggtccttgat atccagggcc gggtggtggc gctgaacgcc ggcgccaacg tccagtccgc
     2581 gtcgagtttc ttcctgccgc tgggacgcgt gacccgggcg ctggagcgca tcattgccgg
     2641 cgagccggtg acgcgcggca ccctgctgac cacgttcacg catgtgccct acgacgagct
     2701 cggccgcctc ggtctcgacg cagcgacgga agcacagtac cgccagctgt tcccacgcca
     2761 gaaaggcctg ctggtggtgc gcgacctggt cccgggggcg ccggccgagg gccagctgga
     2821 gcccggcgac atcgtgctgg cgatcgcggg caagccgctg gccacgttcg aggcgctggc
     2881 cgaagtgctc gacgagcagg tcggtgagcg catcgagatc gcggtgcgcc gcggcagcac
     2941 ggaccaccag ttcgtgatcg aggtcaccga tctgcatgcc gtgtccccgg cggaattcct
     3001 gcagatcggc gacgccgtgc tgcacgacct ttcctaccag caggcgcggc acctgaacat
     3061 gccactggag ggcgtttaca tcgccaaccc gggctacatg ttcatggcct cggcggtgcc
     3121 gcgcggcgcg gtcatcgtgg aaatcaacgg ccagcccacg cccgacctgg acgcgctgcg
     3181 cgcgcttatc gagggcgtgc cggatcgcgc ccgtatcgcc gtgcgctatt acgatttcga
     3241 taacccgcgg gccaccaacc tgcgcagcgt gcgagtcgac cggcgctggt tcccggctgc
     3301 gcactgccgt cgcaacgacg cgctcggcga ctggccctgc gaggcgctgc cggaagtggg
     3361 cgtggcgccg ctgccgccac cggcgacgac cagctttccg cccgagagcg accgccacct
     3421 gcgcacgttg caggcctcgc tggtgatggt caatttcgag atgccgtacc ccgtctccgg
     3481 ggtatctgac cgtttctatc atggcaccgg gttggtggtg gacgccgagc gggggctggt
     3541 ggtcgtcgac cgcaacaccg tgcccgactc gctgggcgac atcacgctga cgtttgccgg
     3601 ttcgctggag acccaggcgc gggttgcctt cgtccacccg ctgcacaacc tcgccgtgct
     3661 ccagtacgag ccgcggctca tcgccgcgac gccggtgcgc tcggcgaccc tgtccgacgt
     3721 gccgttgaag tcgggcgacg aggtccgggt ggtcggactg cgcggcgacc atcggctcgc
     3781 ctcgcagtcc gccaaggtgg ggtcggtcga cgccctcaac ctgccgctgt cccgcacgct
     3841 gcgctttcgc gacaccaacc tcgagacggt ccagctggtg ggcggaccgg acgagttcga
     3901 cggggtgcta gcggacgggc gcgggcgggt gctcggcctg tggtcgagct tcgcctatga
     3961 gtccggtcgg gggaccgagc aggccaacaa gggcgtgccg gcggcgctgg tggccgaggt
     4021 gcttgcgctg gcgcgcggcg agcgtgcgct gcgctccctc gaggccgagt tcctgcagct
     4081 gccgctggcg agcgcgcgcc ggctcggcct ggccgacgag cacgcggcga tgctggaagc
     4141 acacgacccg gagcgccgcc aggtcctgca ggtggtccgg acggtggccg cgacaccggc
     4201 ggcccgcatg ctgcggcccg gcgacctgtt gctgtcgatc aacggcgcca cggtgaaccg
     4261 cttccgcgca gtagaggagg cgagccaggt gccggtggcc caggcccggg tgtggcgcca
     4321 gggcgaggtg ctcgacctcg agattccgac cgtggagctc ggcagcggcg gcgtggatcg
     4381 tgtggtgctg tgggcgggcg cgctgctgca gccgccgcac cgggcgctgg cggcgcagcg
     4441 cggcatcgag ccgtccggcg tgttcgtcgc cttccattcc ttcggttcgc cggccagccg
     4501 caacggtgtg ggcgccggcc tgcgaatcgt cgaggtcgat ggtcagccga cccctgatct
     4561 cgacagcttc ctccgcgccg tggagggtcg tgcccatcgc gccgcggtca ggttgcgcac
     4621 ggtgaactgg aacggccagc cgggcgtgta cacgctcaag ctcgacaacc gctactggcc
     4681 gccctacgag ctggtgcgca acgaggacgg ctggacccgg cgggaccttt agaacaagac
     4741 cggcaaaacc ggggtcagag acgggttttt ctcagaaaaa cccgtctctg accccggttt
     4801 tgccggtttt tcagcccttc agcgtggtgg tgagcccggc caggctgtcc accggcggca
     4861 ggcaggtcgt gccgcggcag atccaggccc gggcacccga ctccggcacg gttttctcag
     4921 ccagcgcggc cggcaggtcc atggccgtcg ccggaacgtc gaacaccagc cggcggggtg
     4981 cgtacaccag ccgggcggtg cgtgcccagt ccgcggcccg cttcggggcg ccgcgcacga
     5041 tgacgatctc gggcggactc agctgctcct cgagcgcgtc gagcagcgca cagtgcgcga
     5101 acggcgcgcg ccggatcacc ggcagcgccg cacgcacggc acgttccgcg gactcgaggt
     5161 aacgtggttc gccgagcagg cagccgagcc tgttgagcgc cagcgccgcg acaccgttgc
     5221 cggacggcag cgcgtcgtcc gccaggggtc gcggacgctc gatgagctgt tcgtggtcgt
     5281 ccgccgtgaa caggaagccg ccgctggccg ggtcctcgaa gtgcgccagc aaggcttccg
     5341 ccagcctgac ggcgaaatcc aggtcgtccg tatcccagcg cagctgcaac atctcgagtg
     5401 cagcgtcgag catgaaagcg tagtcgtcca ggtaagccgc gaggcgcgcg cgcccgtcct
     5461 tccaggtggc ccgcagccgg ccgtccacca gcatttcccg gcgcacgaaa gccatcgccc
     5521 tggccgccga gtccgccaac gcagcatcgc caaggattct tgccgcgacg gccatgccgc
     5581 ggatcatcag cccgttccag gccgccagca ccttctcgtc gcgcccggga cgcacgcgct
     5641 tctcgcgcgc cgcgaacagc ttggcgtgcg cgctggccag gcgcatccgc acctcggcgc
     5701 cgtcgagccc gctgccggcc gcgaactggt ccgcggacat gaaggtgtgc aggtgccagc
     5761 gtccctcgaa gttcgggccg cggtcgaggc cgtggatgcc ggagaccacc gcgaactcgg
     5821 ccggctccag cagccgctgc aactcctcgc gcgtccaggc atagaagcga ccttcctcgc
     5881 cctcggagtc cgcatcgaga ctcgaccagt agccgccctc cggcgcctgc atctcgcgca
     5941 tcacccatcc ggcggtgccg gtggcgatcc ggcgaaacag ctcctcgttg gtgaccgtcc
     6001 aggcctgcgc ataaatcggc agcagctgtg cgttgtcgta cagcattttc tcgaagtgcg
     6061 ggatcatcca gtgagcgtcg accgcgtaac ggcagaatcc acccccgagc tggtcgtaaa
     6121 tgccggcctc cgccatctgc tgcagggtcg aggtcgccat gtagagtgcc tcgaggtccg
     6181 gagactcgcc gagggccgtg gtgcgccagt gacgcatcag gcgctgcagg ttgggcgggt
     6241 gcggaaactt gggcgggccg ccaaaaccac cgtgctccct gtcgaaggtc tcgcccagct
     6301 cacggcgtgc acgcccgagc ggctcgccgt cgagctccgt gccgccggcc tcggccgtgg
     6361 gcgcctccca gaccgcggcg aaagcctcgc gcacgccctc ggcatgctgg cggatttcct
     6421 cgcggtggtc ccgccagtag gcactgacgc gctcgagcag ttcgggaaaa cccggcaggc
     6481 cgtggcgcgg tgtgggcgga aagtaggtgc cgccgaaaaa cggcgtctgg tcgtccggcg
     6541 acaggaacat ggtcagcggc cagccgccgt tgcgctgggt gatcatctgg tgcgccagct
     6601 ggtagatgcg gtccaggtcg ggacgttcct cgcggtccac cttgatgttc acgaacagct
     6661 cgttcatctg cgcggcgatg gcctcgtcct cgaagcactc gtgcgccatg acgtggcacc
     6721 agtggcaggc cgagtagccg atcgagagca ggatgggctt gccctcgcgg cgcgccagct
     6781 cgagggcctg cgcatcccat ggatgccagt ccaccgggtt gtcggcatgc tggagcaggt
     6841 aggggctggt ctcgtcggcg agacggttgc gacgttcagg catgggcaat cccggcgttg
     6901 gaatcgggta gacttgcgcg caactatacc ggaccttccc cggcacttct ccggacgacc
     6961 ttcccttgaa tacgcccgcc gccgacatgc cggaactcaa gctcaagccg cgggaggatc
     7021 gccgcctgcg cgccgggcac ctgtgggtct acagcaacga ggtggatacc gcggcgacgc
     7081 cgctgagcac gctggcgccc ggcgcgatcg tccgcctgag cagcgccgag ggccgtttcc
     7141 tgggctacgc gggcgtgaac ccacacagcc tgatcgcggc gcgcatcctg tcgcgggacg
     7201 cggcccatcc gccggaccga tcgctgctgg tgcatcgcct gaaagtcgcg ctgtcactgc
     7261 gcgagcagct ctacgagcac ggctgctacc gcctgctgtt tgccgacggc gtgccgggac
     7321 tgatcgcaga ccgcttcggc gacgtggtgg tgctgcagtc gggcacggcc gcgatggagg
     7381 ccatgaaggg cgaggtcgtg gacgcggtgc gcaaggtcat tgcgccggcg ggcatcctgt
     7441 ggcgcaacga cgcctcgggg cgcgagttcg aaggcctcga gcgctacgtc gaggtcgccc
     7501 acgggcaagt gcccgaccag gtcacggtca acgaggccgg cgtcgagctg acggcaccga
     7561 tccgtgcggg ccagaagacc ggctggttct acgaccagcg tgacaaccgc acacgattcg
     7621 gccgttaccg gccggccagc gtgctggacg tgttttccta caccggcgcc tggggcctgt
     7681 cggccgccgc gcacggcgca cgcgccacgc tggtggattc ctctgccgca gcgctcgaac
     7741 tggccgctgg tgacgccgac cggctgggcc tcgaggtgga agccttgcag ggccaggcct
     7801 ttgacgtcat gaaggcgctc gccgacgagg ggcgcaactt cgcggcggtg gtcgtcgatc
     7861 cacccgcgtt catcaagcgc cgcaaggacc acaaggcagg gctggccgct tatcagcgcg
     7921 ccaaccagct cgccatgcgc ctgctcgggc gcgacggtct gctggtgtcc tgcagttgct
     7981 ccttccacct gtcgcaggac gacctgctgg ccgtcgtcca gcgtgccgcc cggcatctcg
     8041 gccgcgtggt gcaggtgctg gagatcggcg gccagtcggc cgatcatccc tcgcacccgc
     8101 tgattccgga gacgcgctac ctcaaggcgg tattctgccg ggtgctcgcc ggctgaatca
     8161 ccgctggggc ggatgaaccg gctccggctc cggcgccgcc ttccagcatc ctgcgcagcg
     8221 cgccgaagcg ccggcggtat tgcgccggcg tcatcgcaac cttgcgcctg aacaggcggc
     8281 cgaagaagct ggcgtcggca tagcccacct cgttggcgat ggcctccacc ggtgcgtcgg
     8341 tggtttccag caggtgcttg gcctcctcga tgcgcagggt gtgcacgtac tccagcggcg
     8401 gcatgccggt ggcgcgctgg aagcgtcgct tgaaggagcg ctcggacagc ccggacctct
     8461 gcaccatggc gcccaccggg gcatcctcct cgtagtgctg cgccacccac tcctggcagc
     8521 ggctgatgac ggcatcgtcg ctctggcgct tgcaggacag cgaggcgaag ggctgctgcc
     8581 cgaggtggtg ccagtcgatc aggtgcaggc gcgcgacgcg catggcttcc tccgcgccga
     8641 ggtgccgcgc gacgagatac agggcgagat cgtgccaggc cgtgccgccg ccggccatga
     8701 tgatgcgctg gtcatggccg gtgatcacca gcgcgcggtt cggatgcacg cgcacccgcg
     8761 ggaagcggcg tgccatggcg tcgcagtagg cccagtgcac ggtggcgtcg tggccgtcga
     8821 gcaggccggc ctcggcgagc accaggccgc ccgtgcaggc ggtggcgagc gtcacgccgt
     8881 cgtcgtagcg ggtccggatc cagtcgcgct ccgccaggta gcggtcggcg aggtcctcgc
     8941 ccggcaacac ggccaggtcc ggaatggcga tgacgtccgg cctgggcgcg tcggcgatgg
     9001 cgcaggccgg ttccaccgat agaccgttcg gcaaggccat gcgccgaccg tccaccgaca
     9061 ccacccacgg gttgatccgg gattccgcgc tgcgtccctc gatcaatgcc gcccagtcgc
     9121 ggcctgcgga ggccagcagg tcgcacatgc cgtacagcgt ggatgccgtc gactccggca
     9181 tcgccaggat cgccacctgg agcgcgggtc cgttttcctg catggccgat tcgtccgctt
     9241 gctgcccggt ttgactttag tccattgcgc ggtcgacgtg cacattgcat cccgtgcggc
     9301 gccggggtgg cgccccgaga gaggagtatc gacatgccca cgccatggga aatcctgctg
     9361 gaccccttgt cattgaccat catcggcctg ttcgccgggc tcgccacctg ggaagcgctg
     9421 gcgccgcgcc gcgtgctgcc ggcgatgccc ggctggcgcc tgcgcggcct cgcggccttt
     9481 gccgtgttct tcttcctgtc gtcctacctg ccgatgctgt gggacgacca tctggcggcc
     9541 tggcaattgt tcgacctgag cggtctcggc gtcgcgggcg gcgcgctggc gggcatgctg
     9601 gtgagccagt tgctgggtta ctggtggcac cgcgccttgc atcgctctga caccctgtgg
     9661 ctcggcctgc accagatgca ccatagcgcc gagcggctgg atgcctggag cgcctactgg
     9721 ttcagcccgc tcgatatggt cggctggacc gcggtcggca gcctggcgct ggtgctggtg
     9781 gtgggcgtcg acccgcgcgc ggcgaccctg gtgctgttgc tgacgtcttt cctggacatc
     9841 ttcacgcatg ccaacgtccg cacgccgcgc tggctcggct acctggtggc gaggccggag
     9901 atgcacgcgg tccaccacac gcgcggcgtg catcgctaca acttcgccag cctgccggtg
     9961 ctggacatgc tgttcggcac ctaccgcaac ccacggacct gggatcatca gacaggcttc
    10021 tacgacggcg cctcgcggcg ggtgggtgcc atgttgctat tccgcgacgt gagccagccc
    10081 gaagggcctc ccagggaacc cgccgaggcg tagcgggacc ggggcggcac aggcccgctg
    10141 agggagctat gacttcgccg agcgaagcgg gcctgtgccg tggcggtccc gcggtcccgc
    10201 ggtcccgcgg tcccgcggtc ccgcagtcgc gcggtccagc gagcgcagtg cgctggtaca
    10261 ctacggcgcc atgattccct acccgaacat cgatccggtc gcgatccagc tcggccccgt
    10321 ggcgatccgc tggtacggcc tgatgtatct catcggcttc gccatcgcgt ggttcctggc
    10381 gcgccaccgc gcgaagcagc cgggctccgg ctggacgccc cagcagatcg acgacctgat
    10441 cttctactcg gcgctgggcg tcatcgtcgg cgggcggctc ggctcgatcc tgttctacaa
    10501 cttcgacgcc tggttgcgcg acccggtgat gctggtgcgc gtgtgggagg gcggtatgtc
    10561 cttccacggc ggcctgctgg gcgtggtgct ggcgatgtgg ctgtatggcc gcaagctcgg
    10621 ccgcggtttc ttcgcaatga ccgatttcgt ggcgccgctg gcgccgctgg gcctggcatt
    10681 cggccggctc ggcaacttca tcaatggcga gctgtggggc aagcccagcg acgtgccctg
    10741 ggccttcgtg gtcgacgggg tggcgcgcca tgcgtcgcag ctctaccagg ccgccctcga
    10801 gggcgtagcg ttgtttctca tcctgtggtt cttctccgcc cgtcggccgc cgcgcatggc
    10861 ggtgtcgggg atgtttgcgc tgggctacgg tgtgttccgt ttcctggtcg agttcgtgcg
    10921 cctgccggac gccaacatcg gctacctggc cttcggctgg ctgaccatgg gccagctgct
    10981 gtccgtgccg ctgatcctgc tgggcgcggt gctgctgtgg ctggcgtatc gtcccgcggc
    11041 acgggcggcc tgatcgatgc agcagtacct cgagctgttg cgccgggtcc gtggcgaagg
    11101 cctgcagaaa ggcgatcgca ccggcaccgg caccttgtcc gtgttcggtc accagatgcg
    11161 cttcgatctt ggcgccggct tcccgctgct gaccaccaag aagctgcaca tccgctccat
    11221 cgtgcacgag ctgttgtggt tcctgtccgg cagcaccaac atcggctacc tgaaggaaca
    11281 caaggtccgc atctgggacg agtgggcgac cggggagggc gagctcggcc cggtctacgg
    11341 ccggcaatgg cgcgcctggc cgaccgccga cggcggcgcc atcgaccagg tggccgaagc
    11401 cgtcgacacc atccgccggc ggccgaactc gcgccgtatc atcgtcatcg cgtggaatcc
    11461 cgccgagctg cccgacgaga ccgtcagccc gcaggacaac gtgcgcgcgg gccgcatggc
    11521 gctggcgccg tgccattgcc tgatccagtt ctgggtgggc gacggcaggc tgtcctgcca
    11581 gctctaccag cgcagctgcg acgtgttcct cggcgtgccg ttcaacatcg cctcctacgc
    11641 gctgctcacg cacatggtcg cgcagcaatg cgacctgggc gtgggggatt tcgtatggac
    11701 cggtggcgac gtgcacctgt acctgaacca cctggagcag gccgacctgc agctgcaacg
    11761 gagcccgggg ccgctgccgc ggttgcggat tctccgccgg ccgccgtcga ttttcgatta
    11821 ccgctacgaa gacttcgaag tggtcggcta cgaggcgcgg ccgcacattc cggcgcccgt
    11881 ctcagtctga gccggcgccg cggacgaggt cgctggaccc gggtcgtcag ccggcaggca
    11941 gcgctctcag gcgcagtccg ttggcgtcct cgatccgcgt cgtgacctcg atgccttccc
    12001 gtacactctg ggtgacgata cagaattcct cgaactgcgc caggatgcga tcgagattgc
    12061 ggtagtcgtc ggcggcgtcg ggcaggtgaa tggtcacctc cgcgcgggga atgcgccagc
    12121 gacccgccgc gttgcgctct cgcacggccg tgacggtggc gcgcagccgc gcgggctcgt
    12181 tgcggaactt ccgcattgcg aacagcaggc tggcgctcag gcagttggcg atcgccgcca
    12241 gcagtaactg cgagggattc ggaccgctgc cctcgccgag cggcggcggc tcgtccgtca
    12301 gcagtggctc gagttcagcg ccctcgaaac ggatgctgaa ctggtagtcc tgctgtagct
    12361 cgagcgtgag ctgtatggat tgtgattcgt ccatttcaat gtctccggcc ggcaagcccg
    12421 gtggaagcgg cagggtgcgc gcgcagaggg ctcgtggccc gctcgtggtg gtcggggtgc
    12481 cgtcagtaaa tatcagccta cactaatatg gatgccggcc agtcatctgg ttcctggcac
    12541 gatgttaagg taatcgtcat cggcaatgac ggccgcggat cgccggcatg ccgccgcccg
    12601 aggacgccca catgtcgatc ctgcagccaa tgcttttgcc gatgccggag atatccctgg
    12661 tcgccgccgt cgccgataac ggagtgatcg gcgcggacgg gggcatgccg tggcacctgc
    12721 cggccgacct cactcatttc aagcagctca ccatgggcaa gcctgtcctc atggggcggc
    12781 tcacctggga gtccatcggc aagccgctgc cggggcgcct gaatctcgtg ctgacgcgtg
    12841 acccggcctg gcaggccgat ggcgcccagc gtgctgcctc gctggatgat gccttgcggg
    12901 cggcgcagcg atccggcgcg gtggagttga tggtcatcgg cggtgcggac gtctatcgac
    12961 aggccttgcc gcgtgcgcac cgcatctaca tgacgcgagt gcatgccgaa ccgtggggcg
    13021 acaccctgtt ccccgagctc gagccgggcg actgggagga agtcgcgcgc cgcgagcgat
    13081 tgtcggatga gcgcaatcca tgggatctca ctttcgtggt gctggagcgc gtcgccggcg
    13141 actgagggac cgcggcgagc gccatgctag actccggccg aaacccggac gggacggcga
    13201 gtgaagaacc tgctggccga attgcagcga cgcaacgtga cccgcgtggc gatgctctac
    13261 gtcgtcgccg cgtggctgtt actgcaggtc gccgatgtcg gaatctcaac cctcgggctg
    13321 ccggagtgga gcggccggct ggtgttgctg ctgcttgcgc tgggcttgcc cgtggcgctg
    13381 gtgttcgcct gggtctacga gttgacgccg gagggcatca ggcgcgaatc cgaggtgccg
    13441 ccggccgagt cggtggtcgg ccagacggcc cagaagctca atgtcgcgat catcgtgctg
    13501 ctggtgatcg ccatcgcggc cattgcgctg gacaggctcc tgcccggcgc gccgtcgccg
    13561 gcggaggccg gggacaccgc agagacgcag gccgagccct cgtcgcccgg ggctgccgaa
    13621 gccgcgaccg tggcggctgc acccatgcag gccccggtca ccgagacgag catcgccgtg
    13681 ctgccgttca tcgacatgag cccgggtcgt gacaacgagt acttttccga cgggctgacc
    13741 gaggagctgc tgaactccct ggtcaagatc cgcgcgctcc aggtggccgg gcggacatct
    13801 tctttcgcct acaagggccg cgacctggat atgcgggtca tcggtgacga actcggcgtg
    13861 gcgcacctgc tggaaggcag cgtccgcaag gccggcaatc gactgcgcat caccgcgcag
    13921 ctcatcaagg tggcggacgg ctatcacctc tggtccgaga cctacgatcg cgagctcacc
    13981 gacgtgttcg ccatccagag cgacatcgca gagcacgtcg tggaggcgct gcagattacc
    14041 ttgctcggcg aagatgcagc acgcatgcgg gccggcggca ccaccgacgt cgaggctttt
    14101 aatgactacc tgcagggcgt ctacctggca aaccagggct cgcgggagca gtcggtgcgg
    14161 gcggcggtgg cggcgttcga gagggcgctc gcccgggatc cggccttcgt ggacgcctgg
    14221 gtcgccctgg gggccaccat gagccacatg aacgccaatg catgggaatc tccggaattg
    14281 gcgtgggcac ggatcgagga agcggcgacc gaggcgcggc gacatgcgcc gcagctggcg
    14341 ggcggctaca tgctggagtc gttgctgcac ggctttcggg atcggcggtg gatggaggcg
    14401 atcgaagcga tgcgcacagc ggtggcgctg gaaccgggca atgccaacct gctgcttgct
    14461 tacagccagc ttgcaagcca aatgggcctg cacgaggaaa gcgtccgcgc cgcacagcgc
    14521 gccgtcgaat tggagccgac gaatctcacc caccaggcct acctgggcag gggtctgcag
    14581 ttcgccggcc gttgtcccga ggccgaggtc gtgctcagac gggcgatcga gcgcgatccc
    14641 cggtttcccc gtccccgtta ttacgccggc atctgccgtt acctcgcggg ggaccctgaa
    14701 gcggcgctcg cgctgttcga ggctgagcct ttgccctgga tgcgacgcgc tgggcggccg
    14761 ttggcattgc acgggctggg ccgcctggat gaggcccagg ccgcatggga cgagctggtt
    14821 gcagctcatg gcgacacggc ggccttccag aaagccgaaa tcgccgcgca atggggtgac
    14881 aatgagcttg tgttccttag cctcgagcgc gcctttgaga cgcgcgacgc cgggctgaac
    14941 ctcctgcttg tcagcccctt gctggcgccg ttgcgcgacg acccgcgcta cctgcagatg
    15001 gtggagcgca tgggcatggc gccgctgcta gagtagtgat gtcacggcgg ccgcgcgggc
    15061 ggtcgttcga cccagggatg cggcggtgac cagcgaaaca ctccagtacg gcacgatggc
    15121 gctcggcctg ttcggcggcc tggcgctgtt cctgttcggc atggagcaga tgaccgtcgc
    15181 gctcaaggag gcagccggcg gccgcatgaa gtcgctgctc gcgcggatga cgaaaaaccg
    15241 ctttcgcgct cttgccgccg gcgccttcac cacggcagtg atccagtcgt ccaccgtgac
    15301 cacggtgttg accgtgggtt tcgtacagtc cggcctgatg agcctgcaac aggccatcgg
    15361 catcgtcatg ggcgccagca tcggcaccac cgtgacggcg cagatcgtcg ccttcaaagt
    15421 caccaacctg gcgctgctgg ccatcgcgct cggcttcctg gcccacacgg cagcacgcag
    15481 cgaacgcctg cggcgctacg gcaccatggt gctgggcctc ggcctggtgt tcctcggcat
    15541 gaacctgatg ggcgaggcga tggcgccgct gcgcgactgg ccgcccttcg ccgcggcgat
    15601 gcagcagtat tcctggccgc tggcggcggt ggtgataggc gcattgttta ccgccctggt
    15661 gcaaagctcc tccgccacca cgggcgtcat catcgtgatg gcggggcagg gcatgattcc
    15721 gctggagacc ggcctggcgc tggcgctcgg tgccaatctc ggcacgacca tcacggcgct
    15781 gctggcatcc atcggcaagc gggccgaggc cgtgcagacg gcgctggctt atttcattta
    15841 taagctcgtc acctcggccg cctggctgtt gctgctgccg acgctggccg cggccatgcg
    15901 ggtcgtctca ccgacttttc ccgagctcga cggcatcgaa cggctcgggg ccgagacgcc
    15961 gcgccagatc gccaacggct acctgctgtt gaacatggcg agcgccgccg tgtgcatctg
    16021 gttcaccggg ccgctggccc gcctggtaga gcgcctgcat ccgggccggc cggagcccgt
    16081 gccgacggtg gagcagccga agtacctgga cgatgccctg ctcgatacgc ccgacctggc
    16141 gctcgaccgt gtccgcatgg agctggggca cctcggcgag acggtgctgc gcctgctggg
    16201 gcgcgggccg gcaatggtat acgaaggcag gtccgagacc atcgacgaaa ttccgcgcct
    16261 ggacggcgac gtggacagcc tgcatgacgc catcatcagc tacctgggcc gactgtcgca
    16321 gcggtcgatg aatgccgcgc agaccgagct gctgcacgac tacatggcgg cggccggatt
    16381 gttcgagtcc atgggcgaca tcgtggagat gaacctgtcg cacgcggcgc gcatccggct
    16441 cgccgccggg ctcgaggtca gcgcagcgac ccgtgaagtg atcggccagc tgtgggacaa
    16501 ggtgtactgg agcgtcggcc aggccgcgcg cgcagtggca tcctcgtcag aggaagcggc
    16561 tcacgccgtc atcgagtcca aagacgaggt caatcgcctg gtgcgcgccg ccgagctgca
    16621 cctcgcgcgt cgcctgagtg cgtcggaggc gcatcggctg tcgctgttcc gcatcgaaag
    16681 cgacatggtg gaggccgcca agcgaatcca ctattttgcc cgccgggcgg ccaagcgcat
    16741 ggtcgaaatc gacatgccgg ccgagccgga ccgggggcaa ggcgagccgc ccgatccggc
    16801 cggtccggtg gctacaggga gcggaggtgc gccaccatcc gcttgagcat gtcggcgtcc
    16861 ggcagcctgc cccgggccgc gcccatattg tcgatcatgt ggcgcgcgcg gctggtggcc
    16921 ggggtggccg cggtcaccgc cgggtgcgcg agcacgaact tcaggaaaaa ctgcccccag
    16981 gatgcggcgt cgaattccgc cgcccatgcc ggcacctcgt gcccccgcac ccgctcccag
    17041 agacgcgtgc gcccgaaggg cgcgtagacc agcacgccga tgccgcggtc ctgcgccagc
    17101 ggcaggatgg tttcctccac cgccaggttg tcgatggcgt agtcgatgcc gatgaagtcc
    17161 agcggttcgc tgcgcatcac ttcgatcagc ccggcgtact gcgcggggaa ggtggtcgtc
    17221 acaccgatgt agcggacccg gcccgcctcc ttgtactcct tcaacaggcc gagctgggtc
    17281 ggcacgtccg ccatgttgtg gacctggatg aggtcgatcg tctcgcggcc gatacgccgg
    17341 aaggattcct ccaactgcgc cttggctgcg gctggatccg cacggccccc gtcccgtccg
    17401 gccacgttga gcttggtcgc ccagaagacc ttctcggtca gcccgagctc gcgcgcgatg
    17461 gcacccgcca cctgctcgga ggcgccgtag gacggcgcgg tatcgaaaat cgtgccgccg
    17521 tgctcgagca tcgcgccgat gacctcgcgc agcgccgaga cgtcttcgct gcgtgccacc
    17581 gcggcgaagg tcgcggagct gcccaggccg accgcgggca cgcgttcgcc ggtggcagga
    17641 atcttccggg tgatcggttc cagcgcccgc tcggccgctg ccagcagcgg tcgcggcacg
    17701 gccagcgcgg cgccggccgc gagcgataat tgcaggtatt ggcgtcgtgt cagcatgtct
    17761 tgctacctcc cgtgttccat tctttcgagt cggtcgggcg cgacaggttc cgtcccgccg
    17821 gccagccggc gctgcatcct gcccagccac ataccgagca ctgcccatgc gacggccagc
    17881 gggacggcca cgctggcaag tgcgaccagc ccccagccga gccggccgag caatccctcg
    17941 gcctgggccc cgaccacgtc gccgccgcga tagatgaagg tgtcgatgaa tgcctttgcc
    18001 ttgtacttct cttcgcggct gacgaccgtg tagagcgtct cccgggtcgg gcgcatgatt
    18061 gcgcgttgca ccgcgcggaa ggcggcctcg aacgtgatca gtgctgcgag tgacgcgacc
    18121 agcgccagcc cgatgaagcc cagcgccacg gtcagcggga gcagcgccat ggtcaggggc
    18181 acgccgagcc ggcgcatcag ccggccggtg accagcgcct gcaggaccag cgtggcggcc
    18241 tgcgtaatca cgtcgatgcg tgcgaaccag gcagtgcgca ggtcgatgtc gtctcccagc
    18301 gccgccacca tctgcaggcg agtgaagtag atgaaggtgg agatgacggc caggatcagt
    18361 gcataggcag cgatccccgt gaggtagcgc gagcgcagca cggcgcggac gccctcccag
    18421 gcactgccgc cgatgacggc ttgctcgtcg accacgggca ccatttccgg ggtgctcggt
    18481 tcagcggcgt cgggacgatg cggctgggtc tgtgcgaccg ccaacgccgc gccgagagcg
    18541 agcaccagga atgcggtcga gacgagcagc agcgccggcg taccgaccga ttgcaccagc
    18601 atcgccgcca gccaggggcc gaatatggcg cccgaagttc cgccgaccgc gatcaacggg
    18661 aagaagcgct tgctctgctc gagcgagaag cggtccgcca tcagtgccca gaacagcatg
    18721 gtgatgaaca ggttgaacac gctgaaccag acgtagaaca cctggccgct ggtcacaccg
    18781 acagccgcgg gcgccgcggt cagcagcagg tagaagccga ccaggctgaa tgcgaaaaaa
    18841 agataggtgg ccgagatgaa ctgcaggcgg cggaagcgac tgaccagcag tccgaacacg
    18901 gggttcacca gcagggtcac caccgcagtg cccatgaaga gccagcgcac ggcctcgatg
    18961 ccgcgctgca tcccgagggc ctcccgggcg ggccgcaaca ccatcagggc cgtcagcacg
    19021 cagaaaaaga aggccgcagc ggcgaggacc gggatccgtt cctcgcggcg cacgttgaaa
    19081 atcctcgcga ccggatcgat catgacgccg cagcatgccc cccggctgcc ggccatgtcc
    19141 agcattgcgg cccgctggcg gctgggtgta cagcttaagt acttgccgca gatttgcgcc
    19201 ggcgatgcgc catcggggcg cggctggtgt tatatgtacc ggctcgacag gggtgggcaa
    19261 gatcgacatg gcgatggaag gcatccggct ggtctggaag gacagtttca actctggcca
    19321 tgcgctgatc gacaagcagc accgcggctt gttcgagctg gccaacgagt tgctcgacgc
    19381 catgcatgca gccgcgccgc gcgacgaagt gctgaaggtc gtggaccgcc tgctgcacga
    19441 tatcgccagg cattttgccg acgaggaaga attgctggag cgtgcgggct acctgtcgct
    19501 ctacgaacac tcgcgtgagc acgccgcgct ggtgggcaag gcccgcatgc tggccgatgc
    19561 cttccggcag ggcacgctgc acattcgcga gttgttccag ttcctcgcct atgacgtggt
    19621 gacccggcac atgctcggca ccgaccgtca tttcttcccc tacatcaacg atcagccctg
    19681 agcggcgtcc ttcgctttct tgagcgtcag cgcggccttc aggccctttt cccggatggc
    19741 gcgcaccgcc acgcgccgct gcgtcgagac gtggaagcgc cggccgtggc ggcgcagcag
    19801 ccgctcgtcg atctgcagtc ccagcccggg tgactgcggt acttcgatgg tgccgtcggc
    19861 acggacctcg atcggcggca tgatgccctc gcgcgcggcc ggcacgaagc ctggcggctc
    19921 gtagggaaac tcgatcagct tgcgccgctc ccaggcggcg aaggcatgca ggttgaccac
    19981 catgttgaag ccgttcgacc aggtgtgggg cgtgaaatcc agcccgcgct tgcggcattc
    20041 cgccatgacc tgccggacga cggtcaggcc tgagaacatg gcgtccggct ggtacttgcc
    20101 gaggctgtcg tgctcgaaca gcggcagcag ctcatgccag gcgccgtgca gctcgccgcc
    20161 tgccagctgg accttgacgc ggcggcgcaa ctcggccatg ccgtgccagt cgtgcatgtc
    20221 cagcggctcc tcgagccatg cgatgccgag ctcgtcgcaa ccgcgcgcga aggccgtggc
    20281 ccgcgccagg tcccagtcgg ggttcggctc gaacagcgac accggccagc cctggttggc
    20341 atcgacgccg atcacgaagt cgtctccgag gtactcgcgc acaccgcgca ccagcgcgag
    20401 atcctcctcc agggtggggt atttcacgcg aatcttgacg gcgccgatgc ccatgcggcg
    20461 gatttcatcc aggtacgggc gtcgctcccc aagcggtcgc agcgagccgg tcgacgcgta
    20521 cacgcgcgcc cggtcgacgg tctcctcgcc gtcctgcaac agcttgtaga ccggcttgcc
    20581 ggccagtttg cccgccatgt cccaccacgc cgcctccagc caccagttgc gccaaccc
//
LOCUS       DAOMPG010000010        19264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1231_length_19264_cov_4.286324, whole genome shotgun sequence.
ACCESSION   DAOMPG010000010 DAOMPG010000000
VERSION     DAOMPG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_19264_cov_4.286324"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLS34_01090"
     CDS             complement(<1..322)
                     /locus_tag="PLS34_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006013642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRP86097.1"
                     /translation="MNKAALILSLAAMATAPAVLADEVIRLSEPVQTGEGYEVFGAPV
                     DSTGDAQRLGEIIAAGEEYAGKQVKATATVAQVCQKKGCFFVAQDGEAVARVTFVDYS
                     FFVPT"
     gene            complement(415..861)
                     /locus_tag="PLS34_01095"
     CDS             complement(415..861)
                     /locus_tag="PLS34_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86098.1"
                     /translation="MKLLERLSAWLAATLVTAVTGSIVQTQFNLAAIAKLGAPVPIAL
                     RLQTTLQDLAGFAPMLAAVAGAGLLVALPVAAWLGRRLARPVLLYTLAGAAVIAVAIL
                     LMNGVLPVTAIAATRSMAGFLALTACGALGGWTFSRLAPQRRSAMA"
     gene            976..2073
                     /locus_tag="PLS34_01100"
     CDS             976..2073
                     /locus_tag="PLS34_01100"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRP86099.1"
                     /translation="MRLLLLLCGALLASACGPAVAVDYRIETVAEGLEHPWSIAFLPD
                     GRMLVTERAGRLRIIENGRLLDEPVRGVPPAHVRSQAGLFEVALDPDYADNGWLYLTL
                     AHGTAAANSTRVVRGRLRDGELRDVEVLFTADPQHNTSVHYGGRMAFLADGTLAVGLG
                     DGFDFRESAQRLDSHTGKIVRIHRDGSVPADNPFIGRAGALPEIYSYGHRNVQGLVFD
                     AQSGRLWAHEHGPRGGDEVNLVEPGRNYGWPVVTFGVDYSGAQITPFRSRPGMEDPRV
                     DWTPSIAPSGMAVYRGEQFPAWQGDLLVTALVAREVRRVALDGDRVTGEQALFGEIGQ
                     RLRDIKVGPDGALYVLTDAPRGKVLRISAAD"
     gene            2122..2616
                     /gene="lysM"
                     /locus_tag="PLS34_01105"
     CDS             2122..2616
                     /gene="lysM"
                     /locus_tag="PLS34_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRP86100.1"
                     /translation="MGLFNFISNAGEKLFGSGDIDENAVLKHITGLGLKIKPFSVVAH
                     QKEKMVSLIGYAETLQDKEKAILAAGNIKGVEKVDDRLKVGAPPAKAAAQATAVEPPE
                     PDAAELPDSQFYTVKSGDTLSKIAREFYGNANKYPVIFEANKPMLSNPDRIYPGQVLR
                     IPKS"
     gene            complement(2714..3124)
                     /locus_tag="PLS34_01110"
     CDS             complement(2714..3124)
                     /locus_tag="PLS34_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRP86101.1"
                     /translation="MANYKIDDLEGVGEVYAAKLTAAGIKDTDKLLAACKTAKQRADL
                     ATRTGISAKRLSKWANMVDLYRIDGVGSEFAELLEAAGVDTVPELAQRNAANLAAAMA
                     KTNEAKKLARRTPSETEVAKWVAQAKKLPRALEY"
     gene            complement(3284..5560)
                     /locus_tag="PLS34_01115"
     CDS             complement(3284..5560)
                     /locus_tag="PLS34_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP86102.1"
                     /translation="MSAFLPARGWLSRLAISLVILAPVCHGHAADPPTEPQAGVERPR
                     IGLVLGGGGARGGAHIGVLKVLDELRVPVDCIAGTSMGALVGATFAGGAPAERIEREF
                     LAIDWTAALGLRGERALMPMQRKLAGITYSNSIQFSISDGRLRGDGGVLATQNVEELL
                     RLLVGAARWIDDFDALPIPYRAVATDLGKGEMVVLGSGDLTRAMRASMAVPGVFAPVV
                     VEDQVLVDGGMMRNLPVDVARELCADVAIVVAIDLPLPPTEELQSIFALTGRSIDAMI
                     IANERAQLATLTADDVAIRVPVGDISAGDFARVHETIPLGEAAARSVADSLRRYALPE
                     EDYRRWRDRVSTPEAAPLRIGEISFSPLQYASEEYLATRMETHAGDEVTLEELERDMS
                     RIFASGDFARVDYRLRPQAAGGHLLQVDVIERPGGRDFLRFDLGLSGSSGGDVLFALR
                     ADHRREWMNALGGQWRNALQLGQISELTSAFYQPLDVPQRFFVEAGLAGRRTLEDIFD
                     DGDRVARFDLLQSEAQFDLGVNLGNHTRVRSGLRWGVHEFKLDIGTAAIVDEATTRDA
                     SVTLGAVYDTRDAAALPSKGSYVGVEAARSGGWLGGEQSYEVVEAVAGHTTRWGDMLL
                     VLAAGAGRTLGGNLPRYRDFQVGGVRSFPALSRGELRGEDYWSGSATWLLQVGDSRAL
                     FGQMFFAGIGLQAVNVSGQLDGIDEGTILGASLTVGARTLAGPLLLSLGAADNGSVTL
                     HFSLGRPISEGSMLDRLH"
     gene            complement(5575..6081)
                     /locus_tag="PLS34_01120"
     CDS             complement(5575..6081)
                     /locus_tag="PLS34_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HRP86103.1"
                     /translation="MGTVGWLLVVAVGMALGYSVGRLWPGSAARITALQRERDAARED
                     LRIYREEVSRHFQRTAELFDKVTADYRGLYEHLATSARQLGAIRGEAVDSRLARPEQR
                     RLAEVAPPQPPAAEPGPAPEPEPAADESSAAPAGAPAPPTESTDAGEKPEPPAESPDA
                     GEKPPESR"
     gene            complement(6175..7968)
                     /locus_tag="PLS34_01125"
     CDS             complement(6175..7968)
                     /locus_tag="PLS34_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86104.1"
                     /translation="MTPAQAAIASSSQGAAASRARGDRLPRDLVPLVIGVTGHRDLLP
                     GELPELRRRTREFLADLQAQHPDLPLAVMSPLAEGADRMVAEEARALGIPLIVPTPLP
                     MALYEQDFRTPGSLQQFRELCAEAEVVELPLLPGDTPESVAGQGPRRDAHYARLGIYL
                     CAHCHVLMAVWDGKPSEKLGGTAQVVRFHHWDEMPGFVERKDSSVQILAKDESDLVYH
                     LVCSRNRPDGRPAAGLEPFECWWYTTDEAVPRSKAMPAEYHAIFARAAEFNRHVRQHR
                     ARIRAGGWPLLPADAPPAVAEAAAPVNTMFMAADWLAIHFQSRVTMTLRALYVLAALM
                     GLAFIAYADLPGFDLMVYFFLGFFAAGVLIYRFAERGGWHRKYLDYRALAEGLRVQCY
                     WIAAGVTAGRTTKFAHDNFLQKQDVELGWIRNVMRYTGRLGDIGSPTRAGLDFARREW
                     VGEPHPGGGQLAYYERKALERTRLSRSTAAIGSFCLWAGITVAILLALFGRLMPATGQ
                     DLFIVLMGVLPLVAAVREAYAQKRAEKELIKQYVFMARIFRNARRQLEIAGSDGMRRQ
                     ILKALGDAALEEHAQWLLMHRERPLEHGRMS"
     gene            complement(7993..9462)
                     /locus_tag="PLS34_01130"
     CDS             complement(7993..9462)
                     /locus_tag="PLS34_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86105.1"
                     /translation="MLIRTLARMAAIPALALVPTVLLAALAPPQPEFGPIVEFDGRHY
                     QVVRSDGIDWDAANDIANASVYDGVSGHLATLTSRAEDEFVDQLRRDSGLDRPEVWVG
                     GVQQSCAPEAAGCGWHWVNGEAAISTPQAPLLSYSNWLPGEPNDNYGKGSEQHLAIGL
                     GNAFGWNDEGALGNIGGFVIEYDTARIVDPNQCIEGDGCETTAGQVLLLPPVELADNA
                     SIGIRTYEFTDDPGRCGVAPLTLFGGSDPRPPLVIPPYLCGSPRFLVVEVSTEGFEVR
                     QGTILVENEPAEALPGNLYECTGPIAQDPLNPDLDPQHRDVVAWQATAAADMLESDLG
                     ASVDPMFAGALGEFTFECGSSRGKIKSASYYVVGMHIDFGEGYDLAGNAAANHERFVA
                     LTRYKLQLLQAAVEESRTNLALQNGDYTKMSTMLRNALRFHDRGQFGSALKSVNQFLK
                     FVDKADYAVIPGENYQGEHDMRGSNLVFMYEEKVIPFAP"
     gene            complement(9609..12077)
                     /locus_tag="PLS34_01135"
     CDS             complement(9609..12077)
                     /locus_tag="PLS34_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86106.1"
                     /translation="MATFRADMYPRCASLPKLVSLKEGAGQYDLMPPTATEIGQMVRL
                     PTRAAGLRFEEDFATNERLDDMLRDAAAARPELLPLLQFTLEELYQRRREDGTLSLEA
                     YRELGGVEGSLARRAETVFKQLPREVQAALPRVLDALINVRREGQEAIGRRRAHQSDF
                     DTVTARDLVNAFIDARLFVTELDDDGQASVMVTHEALLWHWPRVKEWIEQNRENLRVH
                     SRVNAAAELWMREHRRPDLLLPSGKPLEEGKALLESQSTLTSYESSFLQASLAAARKR
                     QYIRHAMVSALGVLALFASLAALFATQQQQLADAERARAETEALTAQRTTDFLVGLFE
                     VSDPSESRGNTITAREIMDRGASRIERELATQPAIQATLMETMGTVYTSLGLYDQASP
                     LLQASLDRRTALYGDHHAEVARSMDRLGEVMKLRADYEPAELMYREALALRRQLLGDA
                     HIDTARSIYELADLLGRKGEYAEAEPLFREALALHRQLRGEQSKETARSLEGLALNLY
                     DQGDFSAAAALLRQAVAIQRQLHDEPHPALAQALNNLGFVVSEIGEYQESEQLFREAL
                     AMKMVLYPGNAHPEIAMGMNNVAFVLHDQRKYEEAEALYREAIAMQRALLGDHHPDVA
                     MGLNNLAFLTHEKGDREAAIQIARHSLDTYRQAVGDEHPLVARSMNNLAMWQMEAGDF
                     AAAEPLLREALELRRRLLGPEHADVAASMTLLAGLLIDTGRFEEALPLAGTARAICLD
                     AFGPDHWRTASAASAEGAALAGLQRYEDAEPLLAASFSVLSNDRGALPFFVSNGGRWL
                     GELYRSTGRSRDAATRYGRQAAGD"
     assembly_gap    12151..12160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12161..13358)
                     /locus_tag="PLS34_01140"
     CDS             complement(<12161..13358)
                     /locus_tag="PLS34_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP86107.1"
                     /translation="MAEINTIRIFISSPGDVFEERTLAQRVIERVQAEFTGRAVLEPI
                     FWEHEPLLATDTFQTQIAMPSDADVMIAILWSRLGTRLPKDFTRADGSRYQSGTEFEF
                     EDAVEGFRKHGKPQLLVYRKTAKASVQLDDEAALLDRIDQKRKLDAFIESWFHHTEDG
                     SLKAAFHPFATPSEFEQVLERHLRKIVQRALPESGRRDDVAAAVWKKGSPFRGLQSFH
                     YEHAPIFFGRTRAIGDVLELLRRQAAGGRAFVLILGMSGGGKSSLARAGVLPMLTQPG
                     VIEGVGHWRRAVFRPSDVRGDLFAGLATAILREPGLRLDGTGIDEFAQVLRTAPAAAV
                     TIIRTALSQCAAERAADTPDQRPPETRLALVIDQMEEIFTQDWVTAEDRERFVDTIDL
                     LARSGRI"
     gene            13494..15470
                     /locus_tag="PLS34_01145"
     CDS             13494..15470
                     /locus_tag="PLS34_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HRP86108.1"
                     /translation="MAESANRVAAEAALGGRIAAAKGDLAGLRALWHQRSDPALGWVS
                     AAGIYRSLARAFIEVGAPLPGLEVAVEGLQACGPDVELRQLQGLALARGGATAEANRV
                     LEALRSEGHLDDQTLGILARTHKDLGLSATGDTRRRHLEAACRGYAAAFDRGAGYWTG
                     INVATLAALLGEHDRSRAVARVVMSNGLAALDSLPPAHPDRYWALATIGEACLVMADL
                     NAAERWYRQAAASGSRRFGDLNSTRRHAVLLLQHLGEDPAAVDAWLPLPKVVVFSGHM
                     IDRPGRENERFPAHMEAAVRAAMTDWLARRNALVGFSSAACGADILFQEALESLGGER
                     HVVLPYLPEEFAADSVDIGVGGDWRNRFDKVLETARVVIASSSRPLAGSVAYEYANHL
                     VHGLGLVRAAELETRLLGFAAWDGAAGDGVGGTASAVSQWLRRGVQVEYLELGPGTAQ
                     PTHELIVRTAAAATTPVSAPASDVSVEGDSSDAVMALLFADAVGFSALSDVEVPLFVS
                     RCLGRIADLVAARHHHVAVRETWGDGLFLAFTSVRAAGLFALDMLDLMQCTDWLAEGF
                     SNPLAMRLALHAGPVHLTEDPITALPKCCGTHVSRAARLEPKTPAGQVYASEAFAALV
                     AMEGVDEFRCDYVKELEWAKRYGTFPAYVVRRRE"
     gene            complement(15498..16898)
                     /locus_tag="PLS34_01150"
     CDS             complement(15498..16898)
                     /locus_tag="PLS34_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRP86109.1"
                     /translation="MRQLLARLAAVAVILSTASCTGDGPDYIWLRAMHAMPDTPLVRV
                     DFENYVFRRTVDFGGATSEGGESLLSGSGGTARMTVEYFGLRNQVAGTLLELDVPVVV
                     DAISTVILAGDFEAPAPLVVISPRRARPLGALYFQFAHATPALGALDVYVTAPDTDLA
                     ATAPVTTIDPLDHSDSVEVPFGDLRIRLAAAGTLDVIMDSGELEFAELTGATGPGAEW
                     LFAVAPSVTSGPAQVQLIGSSGRSSLKVNDAGTTATLRAYHALAGTPAVDLVAATEPE
                     TTLLSGLEFATRSPLVALPGEELEVEFRLTGQPDEAVVSRTLPFVNGSEYGVFLAGTA
                     ATPEILAVETLRRSVTPYARIRFAHLAAGSEFFSVFLTETEDEPPSPDNRVVVDLRNG
                     QVLEYLLVEPGDYFVTFTSRIYENAADADNAEVTVVYGPEPLPLAGGDVFTYAIFAPQ
                     VEGDPETITLFDDHQP"
     gene            complement(17020..17769)
                     /locus_tag="PLS34_01155"
     CDS             complement(17020..17769)
                     /locus_tag="PLS34_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRP86110.1"
                     /translation="MHFPRHPSAPRKLVVLNAKGGSGKTTLATNLAAWLAARGEPTAL
                     MDFDPQGCSTRWLANRPADRPPIHGIAAYRIHQHATRSWQLRTPPGTRYLVVDTSAAI
                     PAQRIAEYTREADALIIPVGATDIDTHAAAGFIADLLLVAKEDRRHGRIAVVANRVRS
                     GTVAQRRLMRFLDTLEIPYLAVLRDSQVYAHAAQLGLGIHEFKQHQVRRDLEQWAPLL
                     AWVERRCTPDPAAGTTGQDRGRLAEVHTIGS"
     gene            complement(17864..18145)
                     /locus_tag="PLS34_01160"
     CDS             complement(17864..18145)
                     /locus_tag="PLS34_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRP86111.1"
                     /translation="MSQVTVQIPPPLRPFAAGAAELRVAARTVAAALAAVGADRAALL
                     ARVLTPEGELRPYVNIFVGERNVRHLQGLETKLEDGDVVAIIPAVAGGR"
     gene            complement(18142..18612)
                     /locus_tag="PLS34_01165"
     CDS             complement(18142..18612)
                     /locus_tag="PLS34_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRP86112.1"
                     /translation="MNAEALPGPARRRGDPPPDRLVIRLVPALREAIVAPCIEALPNE
                     GCGLLLGRRDGTAAAIVEVLPARNDRASPDRYEIGPEAVLGADRRARDQGLELLGAWH
                     SHPGGAAVPSATDRAEAWPDWCYLIVGLADSQHPELRAWRLLGDDFVEDTLEIE"
     gene            complement(18602..>19264)
                     /gene="yqeC"
                     /locus_tag="PLS34_01170"
     CDS             complement(18602..>19264)
                     /gene="yqeC"
                     /locus_tag="PLS34_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRP86113.1"
                     /translation="IAARHPGRVAFTTTVHTLPPPAALGAVVVIAPEQVLENELRRHG
                     EARFVAYACPGDKPGRLTGVPTARIDALHAACGFDVTLVKADGARMRWVKAPADDEPV
                     LPAGVTTLIPVLSARALGEPLGERIAHRVERLAQVSGAAAGLPFLPEHAARLLASEHG
                     LLKGAGSTRVVPVINMVDDPQRLQLATETAQRALALTARFDRVVLARLKDADPVVAVI
                     ER"
BASE COUNT         2972 a         6528 c         6758 g         2996 t
ORIGIN      
        1 cggtgggcac gaaaaagctg tagtcgacga aggtcacgcg ggccaccgcc tcgccatcct
       61 gggccacgaa aaagcagccc ttcttctggc agacctgggc caccgtggcg gtcgccttga
      121 cctgcttgcc ggcatattcc tcgccggccg cgataatctc gccgagccgc tgcgcgtcac
      181 ccgtgctgtc gactggcgcg ccaaacacct cgtagccctc accggtctgt accggctcgg
      241 agaggcggat cacctcgtcg gccaacacgg cgggggcagt ggccatggcc gccagggaaa
      301 gaatcagagc agctttgttc acgttgacac ctcgtgggag cctggctccc ggcaacctgc
      361 ggggaccgcg cattgcgcga taatagccac gaaggatagc aagccggcag ggactcacgc
      421 catcgcgctg cggcgctgcg gcgccagccg gctgaaggtc catccgccga gcgcgccgca
      481 ggccgtcagc gccaggaatc ccgccatcga gcgcgtcgcg gcgatggcgg tgacgggcag
      541 cacgccgttc atcagcagga tcgcaacggc gatgacggcc gcgccggcca gcgtgtacag
      601 caagaccgga cgcgccaggc gccgccccag ccaggctgcg acgggcaggg cgaccagcag
      661 gccggcgccc gccacggccg ccagcatggg cgcgaatccg gccaggtcct gcagggtggt
      721 ctgcagccgc agcgcgatcg gcaccggcgc gccgagcttg gcgatggccg ccaggttgaa
      781 ctgggtctgt acgatgctgc ccgtcacggc ggtgaccagg gtggcggcca gccaggccga
      841 caggcgctcg agcagcttca tggcgactcc ggacaacgtg gccttgagac atcggtcgcc
      901 atgccggtcg gcaaacggcg cgccagggct gctattgttg cagtacccac ccacgaacgc
      961 atcccaagac gaatcatgcg actgttactg cttctctgcg gcgccctgct ggcgtcggcc
     1021 tgcggcccgg cggtggccgt cgattaccgg atcgagacgg ttgcggaagg cctggaacat
     1081 ccctggtcca tcgctttcct gccggacggc cgcatgctgg tgaccgagcg cgccggccgg
     1141 ctgaggatca tcgagaacgg gcggctgctg gacgagcccg tgcgcggcgt gccacccgcc
     1201 cacgtgcgca gccaggcggg cctgttcgag gtggcgctcg atcccgacta cgccgacaac
     1261 ggctggctgt acctgacgct ggcgcacggc acggccgccg cgaactcgac gcgcgtggtg
     1321 cgcgggcggc tgcgggacgg ggaactgcgc gacgtcgagg tactgtttac ggccgacccg
     1381 cagcacaaca cctcggtgca ctacggcggg cgcatggcct tcctcgccga cggcacgctg
     1441 gcggtagggc tgggcgacgg cttcgatttc cgcgagagtg cgcagcggct cgacagccat
     1501 accggcaaga tcgtgcgcat tcaccgcgac ggcagcgtgc cggccgacaa tcccttcatc
     1561 ggccgcgcgg gcgcgctgcc ggagatctac agttacggac accgcaacgt ccaggggctg
     1621 gtcttcgatg cgcagagcgg ccggctctgg gcgcacgaac acgggccgcg gggcggcgac
     1681 gaggtcaacc tggtcgagcc gggtcggaac tacggctggc cggtggtgac tttcggcgtg
     1741 gattattccg gggcccagat tacgcctttc aggtcgcggc cgggcatgga ggatccgcgc
     1801 gtggactgga cgccgtcgat cgcgccctcc ggcatggcgg tatatcgtgg cgaacagttc
     1861 cccgcatggc agggcgacct gctggtgacc gcgctggtgg cgcgcgaagt gcgtcgtgtc
     1921 gcgctcgacg gcgatcgcgt caccggagag caggcgctgt tcggcgaaat cggccagcgg
     1981 ctgcgcgata tcaaggtggg gccggacggc gccctgtacg tgctgaccga cgcgccgcgc
     2041 ggcaaggtgc tgaggatctc ggccgcggac tgactcgcag ccctggccgg gcgatcgata
     2101 gcgtagtgaa ggagatcaca catgggcctg ttcaatttca tctcgaatgc cggcgagaag
     2161 ttgttcggca gcggcgatat cgacgagaac gccgtgctca agcacatcac gggcctcggc
     2221 ctgaagatca agccgttctc ggtggtggcg caccagaagg aaaagatggt gtcgttgatc
     2281 ggctatgccg agaccctgca ggacaaggag aaggccattc tcgcggccgg caacattaag
     2341 ggagtggaaa aggtggacga ccggctgaag gttggcgcac cgccggccaa ggccgcagcg
     2401 caggccacgg cggtggagcc acccgagccg gacgccgcgg agttgcctga ctcgcagttt
     2461 tacaccgtga aatcgggcga cacgctgtcg aagatcgcgc gcgagttcta tggcaacgcg
     2521 aacaagtatc ccgtcatctt cgaggcgaac aagccgatgc tcagcaatcc cgaccggatc
     2581 tatcccggcc aggtgctgcg cattcccaag tcctgagcgc taccggcggc gtccccgtcc
     2641 gggggacgcc gcccgagtca cttgcccggt ccggccggtg taaaggcagt gtccggccag
     2701 ggcccgatgc cgctcagtat tccagcgcgc gcggcagctt cttggcctgc gcgacccact
     2761 tggcgacttc agtttcgctc ggcgtgcgcc gcgcgagctt cttcgcctcg ttggtcttgg
     2821 ccatcgccgc cgcgaggttc gcggcattgc gctgcgcgag ttcgggcacg gtgtcgacgc
     2881 cggcggcctc gagcaattcg gcgaactccg agccgacgcc gtcgatgcga tagaggtcga
     2941 ccatgttggc ccacttgctg agccgcttgg ccgaaatacc ggtcctggtg gcgagatcgg
     3001 cacgctgctt cgcggtcttg caggccgcca gcagcttgtc ggtgtccttg atgccggcgg
     3061 cggtcagttt cgccgcgtag acctcgccga cgccttcgag atcgtcaatc ttgtagttgg
     3121 ccattgttcg tcttcttctc ccgtgaggtt ggggccgggc gggccggcac tggttccggc
     3181 ggggatcgcc cggagcgatg catgctgcaa agcggcaaac tgcgctcgca gccctgattc
     3241 tatgagcaac cgtgcagata atcgagttgc gcaagtccgg cagtcagtgc aagcggtcca
     3301 gcatcgagcc ttccgagatc ggccggccga gggaaaaatg cagggtgacg ctgccgttgt
     3361 cggccgcccc gaggctgagc agcagcgggc cggccagcgt gcgtgccccg accgtcaacg
     3421 acgcgccgag gatcgtgccc tcgtcgatgc cgtccagctg accgctgacg ttcactgcct
     3481 gcaagccgat gcctgcgaag aacatctggc cgaacagcgc ccggctgtcg ccgacctgca
     3541 gcagccaggt ggcggaaccg gaccagtagt cctcgccgcg caactcgccg cgggacaacg
     3601 ccgggaagct gcgaactccg cccacctgga agtcccggta gcgcggcagg ttgccgccca
     3661 gtgtccggcc ggcgccggcc gcgagcacca gcagcatgtc gccccaacgc gtagtgtgcc
     3721 cggccaccgc ctcgacgacc tcgtaagatt gctcgccgcc cagccagccg cccgagcggg
     3781 ccgcctcgac ccccacgtag ctgcccttgg acgggagcgc cgccgcatcg cgcgtgtcgt
     3841 agaccgcgcc cagggtgacg ctggcgtcgc gcgtcgtcgc ttcgtcgacg atggcggcgg
     3901 tgccgatgtc gagcttgaat tcgtgcactc cccagcgcag cccggaacgg acacgtgtat
     3961 ggttgccgag attgacaccg aggtcgaact gcgcctcgct ctgcagcagg tcgaagcgcg
     4021 cgacccggtc accgtcgtca aaaatgtcct ccagcgtgcg tcgacccgcc aggccggctt
     4081 ccacgaagaa tcgctgtggt acgtccagcg gctggtagaa ggccgaggtc agttcggaga
     4141 tctggccgag ctgcagtgcg ttgcgccatt gcccgccgag cgcgttcatc cactcgcgcc
     4201 ggtggtcggc gcgcagtgcg aacagcacgt cgccgccgga ggagcccgac aggcccaggt
     4261 cgaatcgcag gaagtcccgg ccgcccggcc gctcgattac atcgacctgc agcaggtgtc
     4321 cgccggcagc ctggggccgc aggcggtaat ccacgcgcgc gaaatcgcca cttgcgaaga
     4381 tgcggctcat gtcccgctcg agttcttcca gcgtcacctc gtcgccggcg tgcgtttcca
     4441 tgcgggtggc cagatactct tcggaggcgt actgcagcgg actgaagctg atttcgccga
     4501 tccgcagggg cgcagcttcc ggcgtactga cccggtcgcg ccagcggcga tagtcctctt
     4561 ccggcagcgc gtaacggcgc agcgaatcgg ccacggatcg cgccgccgcc tcgcccagcg
     4621 gaatggtctc gtgcacgcgc gcgaaatcgc cagcgctgat gtcccccacc ggcaccctga
     4681 tggctacgtc gtccgccgtc agggtcgcca gctgggcgcg ctcgttggcg atgatcatcg
     4741 cgtcgatgga gcgaccggtc agcgcaaaaa tggactgcag ctcctcggtg ggcggcagcg
     4801 gcaggtcgat ggcaaccacg atggcgacgt cggcgcatag ctcccgggcg acgtccaccg
     4861 gcaggttgcg catcatgccg ccgtcgacca gcacctggtc ctcgacgacg acgggtgcga
     4921 acacgcccgg caccgccatg ctggcgcgca tcgcgcgggt gagatcgccg ctgcccagca
     4981 ccaccatctc gcccttgccc aggtccgtcg ccaccgcgcg atagggaatc ggcagcgcat
     5041 cgaagtcgtc gatccagcgg gcggcgccga ccagcaggcg cagcagttcc tcgacgttct
     5101 gggtcgccag cacgccgccg tcgccgcgca ggcggccgtc actgatggag aactggatgc
     5161 tgttgctgta ggtgatgccg gccagcttgc gctgcatcgg catcagcgcg cgctcgccgc
     5221 gcagccccag cgccgccgtc cagtcgatgg ccaggaactc gcgctcgatg cgttccgccg
     5281 gtgcgccgcc ggcgaaggtc gcgccgacca gcgcgcccat gctggtgccg gcgatgcaat
     5341 ccaccggcac acggagttcg tcgagcacct tgaggacgcc gatgtgggcc ccgccacggg
     5401 cgccgccgcc gccgagcacc agcccgatgc gtggccgttc gacaccggcc tgcggctcgg
     5461 tcggcgggtc ggcagcatgc ccatggcaaa cgggcgcaag gatcaccagg ctgatcgcga
     5521 gccgcgacag ccaacccctg gcaggcagga aggcgctcat ggcgtggacc gcgcctaccg
     5581 ggactccggc ggcttttccc cggcatccgg cgactctgcc gggggctccg gcttttcgcc
     5641 ggcatccgtc gattcagtcg ggggcgcagg cgccccggcc ggggctgcgg acgattcgtc
     5701 cgcagcgggc tctggttccg gcgccggccc tggctccgcg gctggcggct ggggcggcgc
     5761 gacctcggcg agacggcgct gctcagggcg ggccagccgg ctgtcgacgg cctctccacg
     5821 gatggcgccg agctggcggg cgctggtcgc caggtgctcg tagagtccgc ggtaatccgc
     5881 cgtgaccttg tcgaacagct cggcggtgcg ctggaagtgg cggctcacct cctcgcggta
     5941 gatccgcagg tcctcccggg cggcatcgcg ctcccgttgc agcgccgtga tcctggcggc
     6001 ggagcccggc cagagccgcc ccacgctgta gccaagggcc atcccgaccg ccacgacgag
     6061 gagccatcca acggtgccca tctcacttcc tccctgtcca ggttcatggc gcgcaggaat
     6121 gcgcgcgtcg acggaagtat agcgcgacgc cgaatgcgcc ctgttgcggc gcggctagga
     6181 catgcggccg tgctccagcg ggcgctcgcg atgcatcagc agccattggg catgctcctc
     6241 gagagcagca tcacccagcg ccttgaggat ctggcggcgc atcccgtccg agccggcgat
     6301 ctccagctgg cggcgtgcgt tgcggaagat gcgggccatg aacacgtact gcttgatcag
     6361 ttccttctcg gcccgtttct gcgcataggc ctcgcgaacc gcggccacca gcggcagcac
     6421 gcccatgagg acgatgaaca ggtcctggcc ggtggccggc atcagccggc cgaacagggc
     6481 cagcagaatg gcgaccgtga tgcccgccca caggcagaag ctgccgatcg cagcggtgct
     6541 gcgcgacagc ctggtgcgct ccagcgcctt gcgctcgtag taggcgagct gcccgccgcc
     6601 gggatgcggc tcgcccaccc actcgcggcg ggcgaagtcg aggcccgcgc gagtcggcga
     6661 cccgatgtcg ccgagccggc ccgtgtagcg catcacgttg cggatccagc cgagttccac
     6721 gtcctgcttc tgcaggaaat tgtcatgcgc gaacttggtc gtccgtcccg cggtgacgcc
     6781 ggcggcgatc cagtagcact gcacgcgcag gccctccgcc agggcgcggt agtcgaggta
     6841 tttccggtgc cagccacccc gttcggcgaa ccggtagatg agcacgcccg cggcaaaaaa
     6901 ccccaggaaa aagtacacca tgaggtcgaa gccgggcagg tcggcgtagg cgatgaacgc
     6961 gaggcccatc agtgcggcga ggacgtacag tgcgcgcagg gtcatggtga cgcgcgactg
     7021 gaaatgaatg gccagccagt ctgcggccat gaacatggtg ttgaccggcg cggcggcctc
     7081 ggccaccgcc ggcggcgcgt cggcgggcaa caggggccag ccgcccgccc ggatgcgcgc
     7141 ccgatgttgc cgcacgtggc gattgaactc ggcggcgcgt gcaaagatcg catggtactc
     7201 ggccggcatg gccttgctgc gcggcacggc ctcgtccgtg gtgtaccacc agcactcgaa
     7261 gggctcgagg cccgccgccg ggcggccgtc gggacggttc cgcgagcaca ccaggtgata
     7321 gacgagatcg ctttcgtctt tcgccaggat ctgcacgctg ctgtccttgc gttcgacgaa
     7381 gccgggcatc tcgtcccagt ggtggaagcg caccacctgg gcggtgccgc cgagcttttc
     7441 ggacggcttg ccgtcccaga cggccatcag gacgtggcag tgggcgcaca ggtagatgcc
     7501 gagccgtgca taatgcgcgt cccgccgcgg tccttgcccg gccacggact ccggggtatc
     7561 gcccggcagc agcggcagct ccacgacttc ggcctcggcg cacagctcgc ggaactgctg
     7621 caaggagccc ggcgtgcgga agtcctgttc gtagagcgcc atcggcaacg gtgtcggcac
     7681 gatcagggga atgccgaggg cacgggcctc ctcggcaacc atccggtcgg cgccctcggc
     7741 gagcgggctc atgaccgcca gcggcaggtc cggatgctgc gcctgcaggt ccgccaggaa
     7801 ttcgcgcgtg cgccgccgca gctcgggcag ctcgccgggc agcaggtcgc ggtggccggt
     7861 cacgccgatg accaatggca ccaggtcccg aggcaggcga tcgccacgcg cgcgcgacgc
     7921 tgcggcgcct tgagagctgc tggcgatcgc tgcctgtgcc ggagtcatcg cgaggatgat
     7981 acctcgcgcg gcctacggcg cgaacgggat gactttctcc tcgtacatga acaccaggtt
     8041 gctgccccgc atgtcgtgct cgccctggta gttctcgccg ggaatcacgg cgtagtcggc
     8101 tttgtcgacg aacttgagga actggttgac gctcttcagc gcggagccga actggccgcg
     8161 atcgtggaag cggagcgcgt ttctcaacat tgtcgacatc ttcgtgtagt cgccgttctg
     8221 cagcgccaga ttcgtccgtg attcctcgac cgccgcctgc agcagctgca gcttgtaacg
     8281 ggtcagcgcg acgaaacgct cgtggttggc tgcggcgttg cccgccaggt catagccctc
     8341 gccgaagtcg atgtgcatgc cgacgacgta gtacgaggcg ctcttgatct tgccccggga
     8401 gctgccgcac tcgaaagtga actcgccgag cgcaccggcg aacatcgggt cgaccgaggc
     8461 accgagatcg ctctccagca tgtcggcggc agccgtcgcc tgccacgcga cgacatcccg
     8521 gtgttgcggg tcgaggtccg gattcagcgg gtcctgggca atcgggccgg tgcattcgta
     8581 caagttgccc ggcagcgcct ctgcgggctc gttctcgacg aggatcgtgc cctggcggac
     8641 ttcgaagccc tcggtgctga cctcgacgac gaggaacctt ggcgagccgc acaggtaggg
     8701 cggaatgacc agcgggggcc gcggatcgct gccgccaaac agggtcaagg gcgcgacacc
     8761 acagcggccg gggtcgtccg tgaactcgta ggtccggatg ccgatcgagg cgttgtccgc
     8821 gagttcgacc ggcggcaaca gcaggacctg tccggcggtc gtttcacatc catcgccttc
     8881 gatacactga ttcggatcga cgatcctggc cgtatcgtat tcgatgacga aaccgccgat
     8941 gttgccgagc gccccttcgt cgttccaacc gaatgcattg ccgagaccga tggcgagatg
     9001 ctgttcgctg cccttgccgt agttgtcgtt gggttcgccc ggcagccagt tcgaatagga
     9061 caacagcggc gcctgcggtg tggaaatcgc ggcctcgccg ttcacccagt gccacccgca
     9121 tccggccgcc tccggcgcgc aggattgctg gactccgccg acccagacct ccggccggtc
     9181 caggccggaa tcccggcgaa gctgatcgac gaactcgtcc tccgcccggg aggtcagcgt
     9241 ggcgaggtgt ccggagacgc cgtcgtaaac gctggcgttc gcgatatcgt tcgcggcatc
     9301 ccagtcaatg ccgtcggacc tgacgacctg gtaatggcgt ccgtcgaact cgacgatcgg
     9361 gccaaactcc ggctgtggcg gagcaagtgc tgccagcagc acggtcggca cgagggccaa
     9421 tgccgggatc gcggccatac gggccagagt acggatcaac atgactgctc cccctggatg
     9481 atgtgtcgca ccgggccagc cggcgacttg tgtgcgcccg gctttgtagc cagccggtgg
     9541 gcacaagagc atcagcctac gcctgaacca tccgcgcagc aattcccgaa tggcaatgcc
     9601 tcgttatgtc aatcgcccgc ggcctgtcgt ccgtatcgag tcgccgcgtc acgcgaacgg
     9661 ccggtcgacc ggtacagttc acccagccac cgcccgccgt tcgagacaaa gaagggcagg
     9721 gcgccacggt cattgctcaa cacgctgaag cttgccgcca gcagcggctc ggcatcctcg
     9781 tagcgctgca ggccggcgag tgccgcacct tcggcgctcg cggcgctggc ggtgcgccag
     9841 tggtcgggcc cgaacgcgtc caggcagatc gcccgggccg tgccggccag cggcagcgct
     9901 tcctcgaaac gcccggtgtc gatcagcagt ccggcaagca acgtcatgct ggcggccacg
     9961 tctgcgtgct ccggcccgag cagccggcgg cgaagttcca gcgcctcgcg caacagcggc
    10021 tcagcggcgg cgaagtcacc cgcctccatt tgccacatgg ccaggttgtt catgctgcgc
    10081 gcgaccagtg ggtgctcatc gccgacggct tgccggtaag tgtcgagcga atgccgtgcg
    10141 atctggatgg cagcttcacg atcgcccttt tcgtgcgtca ggaaggccag gttgttcagc
    10201 cccatggcga cgtctggatg gtggtcgccg agcagggcgc gctgcatggc aatggcctcc
    10261 cgatagagcg cctcggcttc ctcgtacttg cgctggtcat gcagcacgaa cgccacgttg
    10321 ttcatgccca tcgcgatctc ggggtgcgca ttgccggggt acagcaccat cttcatcgcc
    10381 agggcttcgc gaaacaattg ttccgactcc tggtactcgc cgatttcact gacgacgaag
    10441 ccgaggttgt tcagggcctg ggccagcgcc gggtgcggct cgtcgtgcag ttggcgctgt
    10501 atggcgacgg cttgcctgag cagcgcggcg gctgcggaaa agtcgccttg atcgtacagg
    10561 ttcagtgcga ggccctcgag gctccgagcg gtttccttgc tctgctcgcc acgcaactgg
    10621 cgatgcaggg ccagcgcctc ccggaacagc ggttcggcct cagcgtactc gcccttgcga
    10681 ccgagcaagt ccgccagctc gtagatgctg cgtgcggtgt cgatgtgcgc atcgccgagc
    10741 aattggcgtc gcaaggccag cgcctcgcga tacatgagct ccgccggctc gtagtcggcc
    10801 ctcagcttca tgacctcgcc gagacggtcc atcgaacggg cgacttcagc atggtggtcg
    10861 ccgtagagcg ccgtccgcct gtcaagcgac gcctgcaaca gcggggaggc ctgatcgtag
    10921 aggccgaggc tggtatagac ggtgcccatc gtttccatca aggtcgcctg gatcgccggc
    10981 tgcgtggcca gctcccgctc gatgcggctc gcgccgcggt ccatgatctc gcgggccgtg
    11041 atggtgttgc cgcgggattc ggagggatcg gacacttcga acaagccgac gagaaaatcg
    11101 gtcgtgcgct gtgccgtgag ggcctcggtc tccgcccttg cgcgctcggc gtcggccagt
    11161 tgttgttgct gggttgcgaa cagcgctgcg aggctggcga acagggccag gacgccaagc
    11221 gccgatacca tcgcgtgcct gatgtattgc cgtttgcgtg ctgccgccag cgatgcctgg
    11281 agaaaggacg actcgtagct ggtgagcgtg gattggctct ccagcagggc cttgccttcc
    11341 tcgagcggct tgcccgaggg cagcagcagg tcggggcggc ggtgttcgcg catccacaac
    11401 tcagccgctg cattgacgcg gctgtggacc cgcaggtttt cccggttctg ctcgatccac
    11461 tccttgaccc gcggccagtg ccacaacagc gcctcgtggg tgaccatgac cgaggcctga
    11521 ccatcgtcgt cgagctcggt cacgaacagc cgtgcatcga tgaaagcgtt gaccaggtcg
    11581 cgcgccgtga ccgtgtcgaa atccgactgg tgcgctcgcc gccgaccgat cgcttcctgg
    11641 ccctcccgcc gcacgttgat cagcgcatcg agcacgcgcg gcagggccgc ctgcacttcg
    11701 cgaggaagct gcttgaacac tgtctcggcg cgccgtgcca gcgacccctc gacaccaccg
    11761 agttcgcggt aggcctcgag gctgagcgtg ccgtcctcgc gacgccgctg gtacagctct
    11821 tccagcgtaa attgcagcag gggcagcagc tcgggacggg cggccgccgc atcccgcagc
    11881 atgtcgtcga gccgctcgtt ggtggcgaaa tcctcctcga agcgcaggcc ggcggcgcgc
    11941 gtcggcaggc gcaccatctg gccgatctcg gtagccgtcg gcggcatcag gtcgtactgg
    12001 ccggcacctt ccttcagcga caccagcttc ggcaggctgg cgcagcgcgg gtacatgtcg
    12061 gcgcggaagg tcgccaccac ccagatccgc gtggtgcccg atcgcccggg ctggacctac
    12121 aaggtgggtg aagccgccaa attttccgtc nnnnnnnnnn agatccgccc gctgcgtgcc
    12181 agcaggtcga tggtgtccac gaagcgctcg cgatcttcgg cggtcaccca gtcctgggtg
    12241 aagatttctt ccatctggtc gatgaccagg gcgaggcggg tttccggcgg gcgctggtcc
    12301 ggggtgtccg cggcgcgctc ggcagcgcac tgtgacaggg ccgtcctgat gatggtgacc
    12361 gccgcagccg gggcggttct gagcacctgg gcgaactcgt cgataccggt gccgtccagt
    12421 cgcaggccgg gctcgcgcag aatcgccgtg gccagcccgg cgaacaggtc gccgcgcacg
    12481 tcgctgggac gaaacaccgc ccgacgccag tgcccgacgc cctcgatcac gccgggctgg
    12541 gtcagcatcg gcagcacgcc ggcgcgcgcc agcgacgact tgccgccgcc gctcatgccc
    12601 agtatcagca cgaaggcgcg tccgccggcc gcctggcggc gcagcagctc gagcacgtcg
    12661 ccgatcgccc gggtgcggcc gaagaagatc ggcgcgtgct catagtggaa gctctgcagg
    12721 ccacggaacg gcgagccctt cttccacacc gcggcggcga catcgtcgcg ccggccggat
    12781 tcgggcaggg cgcgttgcac gatcttgcgc aagtgccgct ccagcacctg ctcgaactcg
    12841 ctgggtgttg cgaagggatg gaaggcggcc ttgagactgc cgtcctcggt gtggtggaac
    12901 cagctctcga tgaatgcgtc gagcttgcgc ttctggtcga tacgatccag cagtgcggcc
    12961 tcgtcgtcga gctgcaccga ggccttggcg gtcttgcggt agacgagcag ctgcggcttg
    13021 ccgtgcttgc ggaagccctc gaccgcgtcc tcgaactcga attcggtgcc ggactggtag
    13081 cggctgccgt ccgcacgcgt gaagtccttg ggcagccgcg tgccaagccg cgaccagagg
    13141 atggcgatca tcacgtcggc atccgagggc atcgcaatct gggtctggaa ggtgtccgtg
    13201 gccagcagcg gttcgtgttc ccagaagatc ggttcgagca ccgcgcgccc ggtgaactcc
    13261 gcctgcacgc gttcgatcac gcgctgcgcc agggtgcgct cctcgaagac gtcgcccggc
    13321 gaagaaatga agatgcggat cgtgttgatt tcagccatgc tgttcgccgg cccccggtcc
    13381 ggcgtgccgc gtagaatgac gcaggccggg ggatgcagca agctcgacgg cgacgacgcc
    13441 cgggctgcat cgtgttctgg cagcggccgg cggaaggcag ggggatggac ggaatggcgg
    13501 agtcggcgaa ccgcgtcgcc gcggaggctg cgctcggcgg aagaatcgcg gcggcgaagg
    13561 gcgacctcgc gggactcagg gcgctgtggc accagcgctc cgatccggca ctcggctggg
    13621 tctctgctgc aggcatctat cggagcctgg cgcgtgcgtt catcgaggtc ggggcgcccc
    13681 tgccgggcct cgaggttgcc gtggagggct tgcaggcttg cgggccggat gtcgagctcc
    13741 gccaactcca gggacttgcg ttggcgcgcg gcggggccac cgcggaagcc aatcgagtgc
    13801 tcgaagcttt gcggtcggaa ggccatctcg acgaccagac gctgggtatt cttgcgcgca
    13861 cccacaagga cctcggcttg agcgcgaccg gcgacacgcg gcgacgccac ctggaagcgg
    13921 catgccgcgg atacgcggcg gcgttcgatc gcggtgcagg gtattggacc ggcatcaacg
    13981 tcgccacgct ggccgcgctg ctgggcgagc acgatcgctc gcgagccgtg gcccgggtcg
    14041 tcatgtcaaa tggcctcgcc gccctggaca gcctgccgcc tgcccatccg gatcgttact
    14101 gggcgcttgc caccatcggc gaggcatgcc tggtgatggc cgacctgaat gcggcggaac
    14161 ggtggtaccg gcaggctgcc gccagcggat cccgccgctt tggtgacctg aactcgacgc
    14221 gccggcatgc cgtgctgttg ctgcagcacc tgggcgagga tcccgccgcc gtcgacgcct
    14281 ggctgccgct gccgaaagtc gtggtgttct ccgggcacat gatcgaccgg ccggggcgcg
    14341 agaacgagcg attcccggcg cacatggaag ccgcggtgcg ggccgccatg accgactggc
    14401 tggcgcggcg gaatgcactg gtgggatttt cgtcagccgc ctgcggcgcg gacattctct
    14461 tccaggaggc gctcgagtcg ctcggtggcg agcggcacgt agtgctgccg tacctgcccg
    14521 aggagttcgc cgcggacagc gtggacatcg gggtcggcgg cgactggcgc aaccggttcg
    14581 acaaggtgct ggagaccgcc cgcgtggtca ttgcatcgtc cagccgtcca ctggccggca
    14641 gcgtggcgta cgagtacgcc aaccacctgg tccatgggct cggactggtg cgggccgcgg
    14701 agctggaaac acggctgctc ggcttcgcgg cgtgggacgg cgctgcaggc gacggggtcg
    14761 gcggcactgc cagcgcagtc agccagtggc tgcggcgcgg cgtgcaggtc gagtacctcg
    14821 agctcgggcc gggaaccgcg cagcccacgc acgagctcat cgtgcggacc gccgcggcgg
    14881 cgacgacgcc ggtgagcgcc ccggcatccg acgtttcggt cgagggagac tcgtcggatg
    14941 ccgtgatggc gctgttattc gccgatgcgg tcggcttcag cgcgctcagc gacgtcgagg
    15001 tgccgttgtt cgtgagccgg tgcctcggaa ggatcgccga cctcgtggcc gcccggcatc
    15061 accacgtcgc cgttcgcgaa acctgggggg acggcttgtt cctggcgttc accagcgtac
    15121 gggccgcggg cttgttcgcg ctggacatgc tcgacttgat gcagtgcacc gactggctcg
    15181 ccgaggggtt ttccaacccg ctcgccatgc gcctggcgct gcacgcgggg ccggtacacc
    15241 tcaccgagga cccgatcacc gccttgccga aatgctgcgg gacccacgtc agccgggcgg
    15301 cgcggctgga accgaagacg cccgccggcc aggtttacgc cagcgaggcc ttcgcggccc
    15361 tcgtggccat ggagggcgtc gacgaattcc gctgcgatta cgtcaaggaa ctcgaatggg
    15421 ccaagcgcta cggcacgttc ccggcctacg tcgtccggcg gagggaatga agtcggtgct
    15481 gcgggcgctg gcgcccgtca gggctggtga tcgtcgaaca gcgtaatcgt ctccgggtcg
    15541 ccctcgacct gcggcgcgaa aatggcgtag gtgaacacgt cgccgccggc gagcggcaac
    15601 ggctcgggcc cgtaaaccac cgtgacctcg gcgttgtcgg catccgcggc gttctcgtag
    15661 atgcgcgaag tgaaagtgac gaaatagtcg cccggctcga cgagcaagta ctcgaggacc
    15721 tggccgttgc gcaggtcgac gaccacgcgg ttgtcgggtg agggcggttc gtcctcggtc
    15781 tcggtcagga acaccgaaaa gaactccgat ccggccgcaa ggtgcgcgaa gcgaatgcgg
    15841 gcataaggcg tgacgctgcg gcgcagcgtc tcgacggcga gaatctccgg ggtcgcggcc
    15901 gtaccggcga gaaatacgcc gtactcggag ccgttcacga acggcagggt gcgcgacacg
    15961 acggcctcgt cgggctgtcc cgtgagcctg aactccacct cgagttcctc gccgggcagc
    16021 gccaccagcg gcgagcgcgt ggcgaactcc agcccgctga gcagcgtcgt ctcgggctcg
    16081 gttgcggcga cgagatcgac cgccggggtg cccgccagcg catggtaggc ccgcagggtc
    16141 gcggtcgtcc cggcgtcgtt caccttcagg ctgctgcgcc ccgagctgcc gatcagttgc
    16201 acctgcgcag gccccgaggt cacgctcggc gccactgcga acagccattc ggcccccggt
    16261 cccgtggcgc cggtcagctc ggcgaactcc agctcgccgc tgtccatgat cacgtcgagg
    16321 gtgcccgcgg ccgccagccg gatgcgcagg tcgccgaacg gtacttcgac ggaatcggag
    16381 tgatcgagcg ggtcgatggt ggtcacgggc gcggtcgcgg ccaggtcggt gtccggcgcg
    16441 gtcacgtaga cgtccagcgc gccgagcgca ggcgtggcgt gcgcgaactg gaaatacagt
    16501 gcgcccagcg gccgggcgcg acgcggcgag atgaccacca gcggcgcggg tgcctcgaag
    16561 tcgcccgcga ggatcacagt cgagatcgcg tcgacgacca ccggcacgtc cagctccagc
    16621 agcgtgccgg cgacctggtt gcgcaggccg aaatactcca ccgtcatgcg ggccgtgccg
    16681 cccgacccgc tcaacaggga ttcgccgcct tcgctggtcg cgccgccgaa gtccaccgtg
    16741 cggcggaaca cgtagttctc gaagtcgacg cgcacgagcg gcgtgtccgg catggcgtgc
    16801 atggcccgca accagatgta gtcgggcccg tcaccggtgc aggacgcggt gctgagaatc
    16861 acggcgacgg cggcgagccg ggcgagaagt tggcgcatgt tcactccggg gaatgcatca
    16921 ggccgcccgg cgtatggcgg agcggcgtca ggggcgcatt gtaaccgcgc cgccgccgtg
    16981 gcgacgaacc ccgctgccgc tgcggccgat actgcgcgct cagctgccga tggtgtgcac
    17041 ttccgccagc cggccgcgat cctggccggt ggtcccggcg gccgggtccg gggtgcagcg
    17101 gcgctcgacc caggcgagca gcggcgccca ctgttccagg tcgcgccgca cctggtgctg
    17161 cttgaactcg tggatgccga gcccgagctg cgcggcgtgc gcgtagacct ggctgtcgcg
    17221 cagtacggcc aggtagggaa tctccagcgt gtcgaggaag cgcatcaggc ggcgctgggc
    17281 caccgtgccg ctgcgcaccc ggttggcgac caccgcgatg cggccgtggc gccggtcttc
    17341 cttggcgacc agcagcagat cagcgatgaa gccggcggcg gcgtgagtgt cgatgtcggt
    17401 ggcgccgacc gggatgatca aggcgtccgc ctcgcgcgtg tactcggcga tgcgctgtgc
    17461 gggaatcgcc gccgaggtgt cgacgaccag ataacgggta cccggcgggg tgcgcagctg
    17521 ccagctgcgg gtggcgtgct ggtgaatgcg gtaggccgcg atgccgtgga tcgggggccg
    17581 gtccgcaggc cgattcgcca gccaccgtgt cgagcagccc tggggatcga aatccatcag
    17641 ggcggtcggc tcgccgcggg cggcgagcca ggcggcgagg ttggtggcga gcgtggtctt
    17701 cccggagccg cccttggcgt tgaggacgac cagcttgcgg ggcgccgacg ggtgcctggg
    17761 aaagtgcatg cgagggatcc gacttcggaa atcgcctcat aacatagccc taagacaatg
    17821 atattccaag cgccgggcag agcagcgaaa tgctgcagcg caattatcgg ccgccggcga
    17881 ctgcgggaat gatggcgacc acgtcgccgt cctccagctt cgtctccagg ccctggaggt
    17941 gccgcacgtt tcgctcgccg acaaagatgt tgacgtaggg ccgcagttcg ccttccggcg
    18001 tcaggacccg cgccagcagt gccgcgcggt ccgcgccgac ggcggccagc gccgcggcga
    18061 ccgtgcgcgc tgcgacgcgc aactcggcgg cgccggccgc gaagggccgc agcggcggcg
    18121 ggatctgcac ggtgacctgg ctcattcgat ctccagcgtg tcctcgacga aatcgtcacc
    18181 aagcagccgc caggcgcgca actccgggtg ctgcgaatcg gcgagaccga cgatgaggta
    18241 gcaccagtcc ggccaggctt cggcccggtc ggtggcggag ggcacggccg cgccgcccgg
    18301 atgcgaatgc catgcaccga gcaactccag gccctggtca cgcgcgcgcc ggtccgcgcc
    18361 gagcacggcc tcgggcccga tctcgtagcg gtccggggac gcgcggtcgt tgcgggccgg
    18421 caggacctcg acgatcgccg ccgccgtacc atcgcgccgg ccgagcaaca ggccgcaacc
    18481 ctcgttcggc agcgcctcga tgcaaggcgc gacgatggct tcgcgcagcg ccggcaccag
    18541 ccggatcacc agccggtccg gcggcggatc gccgcggcgg cgcgccggcc ccggcaaggc
    18601 ctcagcgttc aatgaccgcc accaccgggt cggcgtcctt cagtcgggcc agcacgacgc
    18661 ggtcgaagcg cgccgtgagc gccagcgcgc gctgtgccgt ctcggtggcc agctgcagcc
    18721 gctgcgggtc gtccaccatg ttgatcaccg gcacgaccct ggtgctgccg gcgcccttga
    18781 gcaggccgtg ttcgctggcg agcaggcgcg ccgcgtgttc cggcaggaac ggcaggccgg
    18841 ccgcggcgcc gctgacctgc gccagtcgtt ccacgcggtg cgcgatgcgc tcgcccagcg
    18901 gctcccccag ggcacgcgcc gacaggaccg ggatcagcgt cgtaacgccg gcaggcagca
    18961 ccggctcgtc gtccgccggc gccttgaccc agcgcatgcg cgcgccgtcc gccttgacca
    19021 gcgtgacgtc gaagccgcag gcggcgtgca gcgcgtcgat gcgcgcggtc ggcacgccgg
    19081 tgaggcggcc cggcttgtca cccgggcagg cgtaggcgac gaagcgcgcc tcgccatggc
    19141 ggcgcagctc gttctcgagc acctgctccg gcgcgatgac caccacggcg ccgagcgccg
    19201 cgggtggcgg cagcgtgtgc acggtggtgg tgaaggcgac ccgtcccggg tggcgggcgg
    19261 cgat
//
LOCUS       DAOMPG010000011        18864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1272_length_18864_cov_4.151736, whole genome shotgun sequence.
ACCESSION   DAOMPG010000011 DAOMPG010000000
VERSION     DAOMPG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_18864_cov_4.151736"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLS34_01175"
     CDS             complement(<1..738)
                     /locus_tag="PLS34_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP86114.1"
                     /translation="MNVELEIRWRIGQDAPREVDPLLFRLLHAIEQGASLRSAAIATA
                     ASYRHAWGLLQGWEQSMGTPLVNLERGRGASLSALGRSILWAERRAHSRLAPFLDSIS
                     AEVEHELAALVATALPLRLVASHDLALGVLKECMNGLRKPKLNVQYRGSLDCVRQLVA
                     GQCDLAGFHVPHGPLGAEIESAYRRLLHPDRFTVLHFAYREQGLMLPPGNPRGIAGIE
                     DLARTRVQFVNRQPDSGTRMLFDCLLKG"
     gene            complement(841..>1628)
                     /locus_tag="PLS34_01180"
     CDS             complement(841..>1628)
                     /locus_tag="PLS34_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP86115.1"
                     /translation="DSAPRAEAVYGESGPVLRLATGSPGELGLIAELAASFSDQEPIR
                     LEWYKAGSGAAVELLRTGQVDLVMVHAPAAEQAAVADGWAGQRTPVGGNAYYLVGPGD
                     DPAGIRDATDALDALRRIAATGSRFLSRGDQSGTHRRELALWAEAGVDPDWPGYTESG
                     DFMAASLRRANAEGAYFLTDNSTWAALQAELPRLERLFSGDPRLVNAYHGLLAPRGPA
                     SALAGRFLEFLAAPAGQSLIREFGREQFGAPLYLDANALPRSP"
     assembly_gap    1629..1638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1688..2233)
                     /locus_tag="PLS34_01185"
     CDS             complement(1688..2233)
                     /locus_tag="PLS34_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRP86116.1"
                     /translation="MLTVKWGIAAAAALALAAGAAADDRRNHDAAASRTLAVGGLVKA
                     PLELKVEALREMPATMIDEIVMCSPRRMKYVGKDYRGVLLRDLLDAAEVDDEALHSRN
                     TIYVVASATDGYQVMFSLQELRNSPIGDQVLVYYEKNGAPLGTDQGLIALVSAGDYQR
                     CHRHVRWLNRIEVHRHASAAP"
     gene            complement(2237..4282)
                     /locus_tag="PLS34_01190"
     CDS             complement(2237..4282)
                     /locus_tag="PLS34_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP86117.1"
                     /translation="MKGTKRVCAAIGLLIGLGHAAFADTDAADKDRPAPDRFDLGVVE
                     VTGNADDVALAYPGVERITNEDMRRHERYDATEALNLLPGVMVENIGNRNERVVFIRG
                     FDSRQVPLFVDGIPVYVPYNGSIDLGRFTTFDLAEIQVTKAFTSVLYGPNTLGGSINL
                     VSRRPSSEFEADVLTGLGFDDDWGKNVHRVAANFGSNQGGWYVQGGASVLDRDDFSVS
                     GDYTPGGAEDGGVRDNSDIKDTKFSLKFGLTPNDTDEYALSYYKQDGVKGTPPYGGQD
                     PSVRSRFWRWPYYDKESLYFLSRTAFGNGHYVRLRAYYDEFDNLLSSFDDNTYTTQDR
                     PFAFNSTYDDHTWGGGAEFGFDLGPAHLLKFAASLKRDFHSEQGDFGEPWEKFEDETV
                     SIGVEDTWTISDATKLIAGTSWNRQDSKRAETLLDDGTILPLPTGGDSAMNYQLGLFH
                     DLSETTEVHFTVGRKTRFPTISNRYSFRFGSALPNPDLQAETSTNFEIGFARDLGRVQ
                     YGGAVFLSQIKDAIQSVSIDDSLCTSPPCEQAQNIEEQDNKGIELYLDVSLTSQLDLH
                     LDYTWLDRENKTRPEVLPIDTPEHKAFGYLVYNPIPMLDIIASIVYEGSRVSESTGAR
                     PVGSFTVANLKGSWEFRNGWIAELGANNVMDEDYAYDEGFPEPGRNFFVNLQYLFGK"
     gene            4618..5190
                     /locus_tag="PLS34_01195"
     CDS             4618..5190
                     /locus_tag="PLS34_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86118.1"
                     /translation="MKITTYCRMGLLAAAAAAVLAAAAPLNAQERPPRDPATETCESY
                     DQDVRGDIALLQQEARQLTSAVDGGAEPPAVEVVRHYAVTLHPQGDVKFVVEPSRVML
                     ADGSFAGHMSFSVPVTGRYRIGLTSESWVDVLAGGDYLDAAAFSGRVGCKPLRKLVDY
                     QLDAGKPYVLMLSGGSEEVMGLAIRPVHEG"
     gene            5198..6586
                     /locus_tag="PLS34_01200"
     CDS             5198..6586
                     /locus_tag="PLS34_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRP86119.1"
                     /translation="MGRAVLSALALAVAAAGAPAHGAGPGESVSLLVRASGGAATSSG
                     DERRVLTARIEGLSEAQRAAARQGEAFFNTSFIPSPAAATRRDGLGPLFISANCRSCH
                     NSLGRGRPPAADNQQPVSLVLQLGAPRGDGSWGDEPTYGPNFNPFAVPGVEPEGSVVV
                     TRTPVRGRYADGDEWELLAPTYRLTDLRYGGLHPHTAISPRLAQPIIGMGLLDAVPEE
                     VILALADPDDEDGDGISGRPNWIDAGDGRRRLGRFGWKANQPDLRAQTTAALFAEMGI
                     TSSDRPEQNCTAVQTACLEGPHGDEPEIADADLADLVLFQRVLAVPPRRALDDPEVIR
                     GAALFREAGCDACHVPRLLTGDVPGLPALSRQEIFPFTDLLLHDMGPELADGRPDHEA
                     SGTEWRTPPLWGLGRAGDLADEVFYLHDGRARSLEEAILWHGGEAAGARARFMAWDRA
                     QRKALVSFLESL"
     gene            6655..7773
                     /locus_tag="PLS34_01205"
     CDS             6655..7773
                     /locus_tag="PLS34_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86120.1"
                     /translation="MAGLLLTAAAPAVHAAYNSHAESDSELFFATYPHLLGTRIDGCD
                     VCHVRMVGLPPGADEGPAVPLSSCDSCHLITDHGHRPGDTLTAFGRDYLEAGRDAAAL
                     SVIGARDSDGDGHANATELAAATDPGDPGSTPEAKPAPRAVVSLEGLRAAGVPAHEQT
                     IFVNVSKSREGDSYSTVRGFRLIDVLAAAGMSASATSIDVISLDGYQATFSIEQLRRS
                     YPQAVPVLGLDKETLGECGWVRYGAGNLAPDEPLADADVLLTFVINGDRYDPAMLAEG
                     GRLRGSGPFRVVAPQMKSPGIPDLSSRADPACLAQVPPRHHYHRDYEKNSDYCVKAVV
                     AIRVNPLPPGTMDVHWPRHAAAAIEDESIIVFGALQDP"
     gene            complement(7790..9046)
                     /locus_tag="PLS34_01210"
     CDS             complement(7790..9046)
                     /locus_tag="PLS34_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRP86121.1"
                     /translation="MKHQNTGCGGHDDPAQTLDEALARITADITPVDGFETVATRDAL
                     DRVLYAPIRSSLDVPAHTNSAMDGYALDGTQLPAEGERGFRVIGTAWAGRPFDGTAGA
                     GECVRIMTGAPVPAGADTVVMQEQVRREGEVAFIGARHKPGQNVRRAGEDLQAGEIAI
                     DAGILLRPAHLGLIASLGVGEVRVRRRPRVAFFSTGDELASIGEALGPGQIYDSNRYT
                     LYGMLQRLGVEVIDLGVVRDRREDLEQAFLAAAAQADVVITSGGVSVGEADFVTETLD
                     KIGEVGFWTVAIKPGRPIAFGRVAGALFFGLPGNPVSVMVTFYQLVQPALRILAGLDM
                     PDAPLRVTATLASRLKKKPGRREFQRGVLSMDDAGRYTVAPAGRQGSGILRSMARANC
                     FIVLPEDCGDVEAGAEVPVQPFSGLC"
     gene            complement(9081..9686)
                     /gene="mobA"
                     /locus_tag="PLS34_01215"
     CDS             complement(9081..9686)
                     /gene="mobA"
                     /locus_tag="PLS34_01215"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HRP86122.1"
                     /translation="MSPPITAVILAGGRATRMGGEDKGLVELAGRPMIVHVLAALAPQ
                     VERVIINANRNLARYAAFGWPVVTDEDSGFLGPLAGLAAGLRAAATPLVLTAPCDSPL
                     VAPDLAERLYSALEDEGAEIAVPHDGERLQPVFALVRRELGDSLAAYLGGGDRKIDRW
                     FAQHRLARVDFSDRPENFVNVNDPEERAAVEARLAARDGKQ"
     gene            complement(9683..10450)
                     /locus_tag="PLS34_01220"
     CDS             complement(9683..10450)
                     /locus_tag="PLS34_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRP86123.1"
                     /translation="MPDPSLRPVTAPSGIAGGNPLMPLEVRGLRYAAGGKAIVDDVSF
                     TVRDAARTVVLGPNGAGKSVLLKLCHGLLRPAAGEIRWAGRTPDQARRRQGMVFQRPV
                     LLRRSVAGNLQHSLAVRGVPRADRVALVDEALQAAGLADRARQAARTLSGGEQQRLAI
                     ARAWVLKPDVLLLDEPSANLDPEATRAIESLILGFQAAGTRVIMTTHDIGQARRLADE
                     VLFLHRGRLLEHAPATRFFAGPANPAAARFIGGGLLA"
     gene            complement(10443..11147)
                     /locus_tag="PLS34_01225"
     CDS             complement(10443..11147)
                     /locus_tag="PLS34_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP86124.1"
                     /translation="MQDFSAAFALAARLVVGLDPELVEIVLLSLYVSVFAVGLAALFG
                     LPLGAVIGMFRFPGRRVVIILLNALMGLPPVVVGLVVYMLLSRSGPLGEFGLLFTPTA
                     MIIAQFILVVPIVAALTRQTVQDLHEEYDEHLRSLDAGRLDSVATLLWDGRFRLLTAV
                     LAGFGRAIAEVGAVLIVGGNILHHTRVMTTTIALETSKGNLALALALGLVLIVLAAGV
                     NAAATLLGDAAHRRHA"
     gene            11389..12765
                     /locus_tag="PLS34_01230"
     CDS             11389..12765
                     /locus_tag="PLS34_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRP86125.1"
                     /translation="MKRPQPSEPLALRNASVLIVGGDAGYRSRLRAALGRHVAFVDSA
                     DGAARAGQLMARCRFGFLVVNAGLSDAPALEWIGGLRQDGAAPRVVLVTERPDRELLL
                     EAMRRGVNDVLERPFEIEAALAALQRVAAIQPAGDRVPMLGEGPGGELQRRELIGDSA
                     AMADVRSLVRRIAPLPGTVLIEGETGTGKELVARWLHAHSARRGAFVAVNCGAIAPEL
                     LESELFGHTKGAFTSAHQVREGLFVAARGGTLYLDEVSEMPLHLEVKLLRVLEEGAIR
                     PVGADQELEVDVRIVASTQHDLRAMVRERRFREDLYFRLNVAQVRLPPLRERRDDIEV
                     LARHFMEQLAGGLGIAPLPMDAQVLAQLQEHHWPGNVRELKNFVERSLMMGRLMIDEL
                     TASAAAEADEVAAYPLDWTLEEVKEHHMARVLRALGGNKSAAARRLGISRKTLERREL
                     PDISGRTA"
     gene            12841..13047
                     /locus_tag="PLS34_01235"
     CDS             12841..13047
                     /locus_tag="PLS34_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HRP86126.1"
                     /translation="MDEEKLNLEIRKFLKVVGITSQREIEQAVREAATAGTLPAGTGV
                     LARVTLELPVLGVRREITQDLDLG"
     gene            complement(13062..13985)
                     /locus_tag="PLS34_01240"
     CDS             complement(13062..13985)
                     /locus_tag="PLS34_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP86127.1"
                     /translation="MDDSQPAGPTEAAALPRFMSVRQVAAYLQINEKKVYALVSAGEI
                     PATKATGKWLFPRDLVDQWLLESSHGGLLADRMLIAGSDDPLLYRAIIHVANEVQARA
                     LISYSSTGTQLGLSLLSRKRADICGIHWGPAEESRQRHPALLRQHPPHHDWVLVRAFL
                     REQGLLLANGLSGTDVTELFSREMRWVMRQEGAGSQRFLHEIIARHGLDPAARRVAGR
                     AFSERDAASMIAMGYADVAIGVRAAATEFGLDFMPMGWEAFDFAMHRGMFFRTLFQKL
                     LDFLRSTECLRVAQKFGGYEFAETGNLIWPV"
     gene            14225..14713
                     /locus_tag="PLS34_01245"
     CDS             14225..14713
                     /locus_tag="PLS34_01245"
                     /inference="COORDINATES: protein motif:HMM:NF023177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HRP86128.1"
                     /translation="MSATPVATFPVGILMARKVVRRSGWSLPSWDVLGVFCGEQFAAP
                     SRTRELVHEADDLQHFLYRGFRIDLYRDAAETYWWNLTGERPSLFVLCLKEDGEELVP
                     NVVTADHEAAQAAVESNGAAFSVPIPPEIHTELEKVVMEHYRPEPPRKRKQHKWHKED
                     KH"
     gene            14710..15375
                     /locus_tag="PLS34_01250"
     CDS             14710..15375
                     /locus_tag="PLS34_01250"
                     /inference="COORDINATES: protein motif:HMM:NF023176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HRP86129.1"
                     /translation="MSGAEDQRKNAAGIQPEEPFLSRWARLKKASREGAGQAPVPAPA
                     AGPDKADVEASNQAEPASTEPEVDAASTAQGGPETLDLPPLESLTPESDFSAFMRPGV
                     DPGLRREALRKLFQNPKYGLLDELDPYRADYAAFTPLGDIITADMKFHAERLLRKQLE
                     EALEAEEADDSSVADTQLPAGDDPDEVPGSIAAADRSEGRDVAAGAGSNNDREDDDEH
                     RNS"
     gene            15356..17035
                     /locus_tag="PLS34_01255"
     CDS             15356..17035
                     /locus_tag="PLS34_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRP86130.1"
                     /translation="MSTETAELPSTGPAGRARAAALALPDAPVSSPTSRVTYQSQGRV
                     VVIGPETESLSAARQLADRVPVTIHVPGVERPDPGTVEGIVIVRGGTPEVEGRLGRFT
                     VNLATPEGAVALAEAAGLEIEHFDLVLDLSPEPLLSHEVPPVGYYAPRGDGRALARAV
                     AELPEMSGEFEKPKYFNFKPELCAHSRSELAGCTRCIDACPTLAITSNDEVVAVDPYL
                     CQGAGSCVAACPSGAMSYAFPTVSDLLTRVRGLLEAFRAAGGDAAGLVLHDGWSRAQV
                     LGEIGAAMPEDLLPLEVEEVGSVGMDAWLAALAYGARGVVICVTDYTPKRMRRELEAQ
                     VQFARGVLAGMGYPPGTLAVANVSKPVEVAAGWAELPDSLRLRPARFVAPDDKRAVLR
                     LAIEHLFHQAPSPRKVAELPPGAPFGEVRVDKAACTLCMGCVSVCPAAALSAGGDLPQ
                     LKFTEWNCVQCGLCEKACPEDAIRLHPRFLFDAEQRQQPRVLHEEAPFCCVSCGKPFT
                     TSGLLDKLQRKLEGHWMFQTEEARRRLQMCEHCRVKDMFRAGHGQGGGLAQ"
     gene            17192..17869
                     /locus_tag="PLS34_01260"
     CDS             17192..17869
                     /locus_tag="PLS34_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRP86131.1"
                     /translation="MTEVAGPGAGAANGAVQPLDEPPRLNAWNLLGRLLAAPPDEAVL
                     ELLAGLDAAQVGGDNLLGAAWEMLAKAAAGTSPAALEEEYQDLFIGVGRGELMPYGSW
                     YLTGFLMEQPLARLRDELRMLGFARREGVSEPEDHAAALCDVMAMISAADDAAVPVEV
                     QSGFFSRHIAPWMGRFFRDMQQAPSARFYRAVGQLGEQYVALEGQYLGAPVKVSGSGQ
                     RPSVGMA"
     gene            17894..18103
                     /locus_tag="PLS34_01265"
     CDS             17894..18103
                     /locus_tag="PLS34_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HRP86132.1"
                     /translation="MSGKSMVDATGRRRVLKTLVAAGGVTALAAVSGRAVAGPGNTSV
                     ERKAPVAQGYRETDHIRTYYSKARF"
     gene            18156..>18864
                     /locus_tag="PLS34_01270"
     CDS             18156..>18864
                     /locus_tag="PLS34_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRP86133.1"
                     /translation="MKLIKKSNVSNSGRPAGDGILGAAIDRRTFLRRTGLVLGGGALA
                     TALQPSMMVRNAEAARVASSAPIELKRSVCGHCSVGCGVIAEVQNGVWTGQEPDFDSP
                     LNLGGHCAKGAAVREHGHGERRIKYPMKLVDGKWKRISWDDAINEIGDKMLKIREESG
                     PDALFLAGSSKASNEGAYLQRKFMAFWGSNNCDHQARICHSTTVAGVANTWGYGAMTN
                     SYNDMQNSKCAFYIGSNA"
BASE COUNT         3013 a         6198 c         6596 g         3047 t
ORIGIN      
        1 gcctttcagc aggcagtcga acagcatccg cgtgcccgag tcgggctggc ggttcacgaa
       61 ttgcaccctc gtccgggcca ggtcttcgat gccggcgatg ccgcggggat ttccgggcgg
      121 cagcatgagt ccctgctcgc ggtaggcgaa atgcagcact gtaaaccggt cgggatgaag
      181 cagccgccgg taggcggact cgatctcggc gccgagcggg ccgtggggca cgtggaagcc
      241 cgcgaggtcg cactggccgg caaccagctg gcgcacacag tccaggctgc cgcggtactg
      301 gacgttcagc ttgggcttcc tgagtccgtt catgcactct ttcaggacgc ccagtgcgag
      361 gtcgtggctg gccaccagcc gcagcggcag cgccgtggcc accagggccg ccagctcgtg
      421 ctcgacctcg gcgctgatgc tgtccaggaa tggcgccagc ctgctgtgcg cacggcgctc
      481 ggcccacagg atgctgcggc ccagtgccga cagcgaggcg ccgcgcccgc gttccaggtt
      541 taccagcggc gtgcccatgg actgttccca gccctgcagc aggccccagg cgtgccggta
      601 ggacgcggcc gtggcgatgg ctgcgctgcg cagcgaagcg ccttgctcga tggcatgcag
      661 caggcggaac agcagcgggt cgacctcgcg cggcgcgtcc tggccgatcc gccagcgaat
      721 ttccagttcg acgttcatgt gcgtgcccgg cccggctggg gtgattggac ttggccgggt
      781 ttataacatg cagccgcgct ccgggcaggt ccggaagcag ttgtctgcgc cgggtcccgg
      841 tcagggcgag cgcgggagcg cgttggcgtc gaggtacaac ggcgcgccga attgctcgcg
      901 cccgaattcc cggatgaggg actggccggc aggggcggcc aggaactcga ggaaccgccc
      961 cgcaagggcc gaggcggggc cgcggggcgc cagcagtcca tggtaggcat tcaccaggcg
     1021 aggatcgccc gagaagagtc gctccagccg gggcagctcc gcttgcagag ccgcccaggt
     1081 gctgttgtcg gtgaggaaat aggcgccctc ggcgttcgcc cgacgcaggc tcgcggccat
     1141 gaagtcgccg ctttcggtat agcccggcca gtccgggtcg acgccggctt ccgcccacag
     1201 tgcaagctcg cgccgatgcg tccccgactg gtcgccgcgc gacaggaagc ggctgccggt
     1261 cgcggcgatg cgccgcaagg cgtccagcgc gtcggttgcg tcccggattc ccgcaggatc
     1321 gtcgccaggg ccgacgaggt aataggcgtt gccgccaacc ggtgtgcgct ggccggccca
     1381 gccgtctgcc accgccgcct gttccgcggc cggcgcatgc accatcacga ggtcgacctg
     1441 tccggttctg agcagttcca ccgcggcgcc ggagcccgcc ttgtaccact ccagccggat
     1501 cggctcctgg tcgctgaagc tggcggcgag ctcggcgatc agtccgagct cgcccggact
     1561 gccggtcgcc aggcggagca ccggcccgga ctcgccatac acggcttccg cccgcggcgc
     1621 agaatcggnn nnnnnnnncc cgcctgcaat gcaggccaag ccggccgccg ccaccaggag
     1681 aaccgggtta cggcgccgcg gacgcatgcc gatggacctc gatgcggttc agccagcgca
     1741 catgccgatg gcagcgctgg tagtcaccgg cggagaccag tgcgatcagg ccctggtcgg
     1801 tgcccagcgg cgcgccgttc ttctcgtagt acacgagcac ctggtcgccg atcggcgagt
     1861 tgcgcagttc ctgcagcgag aacatgacct ggtagccgtc ggtcgcgctg gccaccacgt
     1921 agatggtgtt gcgcgagtgc agtgcttcgt cgtcgacctc cgccgcatcg aggagatcgc
     1981 gcagcaagac gccgcgatag tccttgccga cgtatttcat ccggcggggc gagcacatca
     2041 cgatctcgtc gatcatggtc gcgggcatct cccgcagggc ttccaccttc agttccaggg
     2101 gcgccttcac gaggccgccg acggccagcg tccggctggc ggctgcatcg tggttgcgtc
     2161 ggtcatcggc agcagcgccg gctgcaagag ccaacgccgc tgccgccgca atcccccatt
     2221 tcacggtcag cacggattat ttcccgaaaa ggtactgcag gttgacgaag aagttgcggc
     2281 ccggctcggg aaagccctcg tcgtatgcat agtcctcgtc catgacattg ttggcgccca
     2341 gctccgcgat ccagccgttg cggaactccc atgagccctt gaggttggcc acggtgaacg
     2401 agcccaccgg ccgcgcgccc gtcgattcgc tgacccggct gccctcgtac acgatgctgg
     2461 cgatgatatc cagcatgggt atcgggttgt acaccaggta cccaaaggcc ttgtgctcgg
     2521 gagtgtcgat cggcaggacc tcgggccggg tcttgttctc ccggtcgagc caggtgtaat
     2581 ccaggtgcag gtccagctgg gaagtcagcg aaacgtcaag gtacaactcg atccccttgt
     2641 tgtcctgctc ctcgatgttc tgcgcttgct cgcacggcgg gctggtgcac agggaatcat
     2701 cgatgctcac gctctggatc gcgtccttga tctggctcag gaacaccgcc ccaccgtact
     2761 ggacgcgtcc caggtcgcgc gcgaagccga tctcgaagtt ggtggaggtt tccgcctgga
     2821 ggtccgggtt cggcagcgcg gagccgaagc ggaaggagta gcggttactg atggtgggga
     2881 aacgggtctt gcgacccacc gtgaagtgaa cttcggtggt ttcgctcagg tcgtggaaca
     2941 accccagctg gtagttcatg gcggaatcgc cgccagtcgg cagcggcagg atcgtgccgt
     3001 cgtcgagcag ggtctcggca cgcttgctgt cctggcgatt ccagctcgtg cccgcaatca
     3061 gcttcgtcgc atcgctgatg gtccaggtgt cctcgacgcc gatggatacg gtttcgtcct
     3121 cgaacttctc ccatggctcg ccgaagtcgc cctgttcgct gtggaagtcg cgcttcaggc
     3181 tggccgcgaa cttcagcagg tgcgccgggc cgaggtcgaa gccgaactcg gcgccgccgc
     3241 cccaggtgtg gtcgtcgtag gtgctgttga aggcgaacgg ccggtcctga gtcgtataag
     3301 tgttgtcgtc gaaggagctc agcaggttgt cgaactcgtc atagtaggcg cgcaggcgca
     3361 cgtaatggcc gttgccgaac gcggtgcgcg agaggaaata gaggctctcc ttgtcgtaat
     3421 agggccagcg ccagaagcgc gaacggaccg aggggtcctg tccgccgtaa ggcggcgtgc
     3481 ccttgacgcc gtcctgcttg taatagctca gcgcatattc gtcagtgtcg ttgggcgtga
     3541 ggccgaattt cagactgaac ttggtgtcct tgatgtcgga gttgtcccgg acgccgccgt
     3601 cctcggcgcc gccgggcgtg taatctcccg agacgctgaa gtcgtctcgg tccagcaccg
     3661 aggcgccgcc ctggacgtac cagcctccct ggttgctgcc gaaattcgcg gccactcgat
     3721 gtacgttctt gccccagtcg tcgtcgaagc cgaggccggt aagaacgtcc gcctcgaatt
     3781 cgctcgaggg acggcggctg accaggttga tgctgccgcc cagggtgttt ggcccgtaga
     3841 gcaccgaggt gaacgccttg gtcacctgga tttccgcaag gtcgaaggtc gtgaaacgcc
     3901 ccaggtcgat gctgccgttg taagggacgt agacaggaat gccgtcgacg aacagcggca
     3961 cctgccgcga atcgaagccg cggatgaaca ccactcgctc gttgcgattg ccgatatttt
     4021 cgaccatcac gcccggcagc aggttcaggg cctcggtagc gtcgtagcgc tcgtggcggc
     4081 gcatgtcttc gttcgtgatc cgctccactc ccgggtaggc gagggcaacg tcgtcggcat
     4141 tgccagtgac ctcgaccacg ccgagatcaa accggtccgg cgcgggacgg tccttgtcgg
     4201 cggcgtcagt gtccgcgaac gcggcatgcc caaggccaat cagcaggccg atggccgcgc
     4261 aaacgcgctt cgttcctttc atcctttgtt cccccaagtc ttcgttttct gccaatatgc
     4321 atggactgac ataataggtc caaccaaggc gcattgttct aggagttact ggtcgccgca
     4381 atatgtaaat cacgacataa cttgatgggc caagcctggc accacgcgtg ttgcggcgcc
     4441 tggcagcgtg ctgcgagccg cggcgcagga gaccagcggc attgtcgctg cgaagaacat
     4501 attgcttcgc aacacagtgc tccacataat ggcgggatta catcaaaagc ctaatagaaa
     4561 cttgcagtaa agtgccgcgt cgttgttggc gccgcagcgt gccgagggaa atgcattatg
     4621 aaaatcacaa catattgccg gatggggttg ctggcggccg ccgctgcggc tgttctcgct
     4681 gccgcggcgc cgctcaacgc gcaggagcgg ccgcccaggg atcccgccac ggagacctgc
     4741 gaaagctacg accaggacgt gcgcggtgac atcgcgctgc tgcagcagga ggcccggcag
     4801 ttgacgtccg ccgtggacgg cggtgcggag cccccggcgg tggaggtcgt acgacactac
     4861 gcggtcacgc tgcacccgca gggcgacgtg aaattcgtcg tcgagcccag ccgggtcatg
     4921 ctggcggacg gctcgttcgc ggggcacatg agtttttccg tgccggtgac tggacgctac
     4981 cggatcgggc tgaccagcga atcctgggtc gacgtccttg ccggcggtga ttacctcgac
     5041 gccgcggcct tctccggtcg ggtgggctgc aagccgctgc gcaagctggt ggactatcag
     5101 ctcgacgccg gcaagcccta cgtgttgatg ttgagcgggg gatccgagga ggtcatggga
     5161 ctggccatcc ggccggtaca cgagggctag ggctgccatg ggccgcgccg tgttgtcggc
     5221 gctggcgctg gcagtcgccg cggccggcgc gccggcacac ggcgccggtc ccggcgagtc
     5281 tgtatcgctg ctggtgcgcg cttccggcgg cgcggcgacc tcgagcggcg atgagcgacg
     5341 agtgctgacg gcgcgcatcg agggcctgag cgaggcgcag cgcgccgccg cccggcaggg
     5401 cgaggcgttc ttcaacacca gcttcatccc gagccccgcg gcggcgacgc ggcgcgacgg
     5461 gctgggtccg ctgttcatct ccgccaactg tcggagctgc cacaacagcc tgggacgcgg
     5521 tcggcctcct gccgccgaca accagcagcc ggtctcactg gtgctgcaac tcggcgcccc
     5581 gcggggtgac ggcagctggg gcgacgagcc gacgtatggc cccaacttca atcccttcgc
     5641 ggtcccgggc gtcgaaccgg agggatccgt ggtcgtcacg cgcacgccgg tccgcggacg
     5701 ctatgccgac ggcgatgagt gggagttgct ggcgcccacc tatcgcttga cggacctgcg
     5761 ttacggcggg ctgcacccgc atacggcgat atcgccccgc ctggcgcagc cgatcatcgg
     5821 catggggctg ctggacgcgg tgccggagga ggtgatcctg gcgctggccg accccgacga
     5881 tgaggacggt gacgggattt ccgggcgccc caactggatc gacgccggcg atggtcgccg
     5941 gcgcctcggg cgattcggct ggaaggccaa ccagcccgac ctgcgcgccc agaccaccgc
     6001 cgcgctgttc gccgagatgg gcatcacctc tagcgaccgg ccggaacaga actgcacggc
     6061 cgtgcagacg gcgtgcctcg agggacccca cggcgacgag ccggaaatcg cagacgccga
     6121 cctggccgac ctggtgttgt tccagcgcgt gctcgcggtg ccgccgcggc gcgcactcga
     6181 cgaccccgaa gtgattcgcg gtgcagccct gtttcgcgag gcgggctgcg atgcctgcca
     6241 cgtgccccgg ctgctgaccg gcgacgtccc gggcctgccc gccctgtccc ggcaggagat
     6301 tttcccgttc accgacctgt tgctgcatga catgggtccg gagctggccg acggccgtcc
     6361 cgaccacgag gcctccggca ccgagtggcg gacgccgcca ttgtggggcc tgggacgcgc
     6421 cggcgacctc gcggacgaag ttttctacct gcacgacggg cgcgcccgca gcctcgagga
     6481 ggccatcctc tggcacggcg gcgaggccgc cggggcgcgc gcgcgcttca tggcctggga
     6541 cagggcgcag cgcaaagcgc tggtgagttt cctggagtcg ctctaggacc ggttcgcgtc
     6601 atcaaggagg atcattcgat gcgggaaacg tggtgcacag ggcgagccct cgccgtggcc
     6661 gggctgctgc tgactgctgc tgcgcctgcg gtccatgccg cctacaactc ccatgcggag
     6721 tccgattcgg agctgttctt cgcgacctac ccgcacttgc tcggcacacg gatcgacggc
     6781 tgcgacgttt gccacgtgcg catggtcggc ctgccgccgg gtgcggacga ggggccggcc
     6841 gtgccactga gcagctgcga ttcctgtcac ctcatcaccg accacgggca ccggccgggc
     6901 gacaccctga cggcgttcgg ccgcgactat ctcgaggcag ggcgcgacgc ggcggcgctg
     6961 tcggtcatcg gcgcgcgtga ctcggacggt gacggccatg ccaacgcgac agaactcgcg
     7021 gcggcgaccg acccgggcga cccgggcagc acgccggaag cgaagccggc gccccgggcg
     7081 gtggtgtcgc tggagggact gcgcgcggcc ggcgtgccgg cccacgagca gacgatcttc
     7141 gtgaacgtgt cgaagtcacg ggaaggagac agctattcca ccgtgcgcgg ctttcggctc
     7201 atcgacgtgc tggcggccgc cgggatgtct gcctcggcaa ccagcatcga cgtcatcagc
     7261 ctcgacggct accaggcgac tttctcgatc gagcagttgc gccggtcgta tccgcaggcc
     7321 gtccccgtgt tgggcctgga caaggaaacc ctcggtgagt gcgggtgggt ccggtacggg
     7381 gccggcaacc tggcgccgga cgagccattg gccgacgccg acgtcctgct caccttcgtc
     7441 atcaacggcg accgctacga ccccgcaatg ctggctgagg gcggtcgcct gcgcggcagc
     7501 gggccgtttc gcgtcgtcgc cccgcaaatg aagtcgcccg gcatccccga cctgtcgagc
     7561 cgcgccgatc cggcctgcct ggcgcaggtt cccccacgac atcactacca ccgggattac
     7621 gagaagaact ccgactactg cgtgaaggcc gtggtcgcca tccgcgtcaa tcccctcccg
     7681 ccggggacca tggacgtcca ctggccgcgg cacgcggccg cggccatcga ggacgagagc
     7741 atcatcgtgt tcggcgccct gcaggaccct tgagggtggc gtcgcgcggt cagcaaagcc
     7801 ccgagaaggg ctgtaccggc acctcggcgc cggcctccac gtcgccgcag tcttccggca
     7861 gcacgatgaa gcagttggcg cgggccattg agcgcaggat accggagccc tgccttcccg
     7921 cgggcgccac ggtgtagcga cccgcgtcgt ccatgctcag cacgccgcgc tggaattccc
     7981 ggcggccggg ttttttcttc agccgcgacg ccagcgtcgc ggtgacgcgc agcggggcgt
     8041 ccggcatatc caggccggcg aggatgcgca gcgccggctg caccagctgg tagaaggtca
     8101 ccatcaccga caccgggttg ccgggcaggc cgaagaacag cgccccggcc acgcggccga
     8161 aggcgatcgg ccggccgggc ttgatggcca cggtccagaa gccgacctcg ccgatcttgt
     8221 ccaaggtttc ggtgacgaaa tccgcttcgc cgaccgacac gccgccggac gtgatcacga
     8281 cgtccgcctg cgccgccgcc gccaggaaag cctgctcgag gtcttcgcgc cggtcgcgca
     8341 ccacgccgag atcgatcacc tcgaccccga gccgctggag catcccgtac agggtgtagc
     8401 ggttgctgtc gtagatctgg ccggggccga gcgcctcgcc gatcgaggcg agctcgtcgc
     8461 cggtggagaa aaacgccacg cgcggccgtc gccgcacgcg cacctcgccg acgcccagcg
     8521 aggcgatcag gccgagatgc gcggggcgca gcaggatgcc ggcatcgatg gcgatctcac
     8581 cggcctggag gtcctcgccg gcgcgccgca cgttctggcc cggcttgtgg cgtgcgccga
     8641 tgaacgccac ctcgccctcg cggcgcacct gctcctgcat caccaccgtg tcggcgcccg
     8701 ccggcaccgg cgcgccggtc atgatgcgca cgcattcgcc ggcgccggcc gtgccgtcga
     8761 agggccggcc ggcccaggcg gtgccgatga cgcgaaagcc acgctcgccc tcggcaggca
     8821 gctgcgtgcc gtcgagggca tagccgtcca tggcggagtt ggtgtgggcc gggacgtcca
     8881 gcgaggagcg gatcggcgca tacaacaccc ggtcgagcgc atcgcgggtg gcgacggtct
     8941 cgaaaccgtc caccggggtg atgtccgcgg tgatgcgcgc caatgcctcg tcgagtgtct
     9001 gggccgggtc gtcgtggccg ccgcagccgg tgttctggtg cttcatgccg ttgtcctgtc
     9061 gggcggccgc agccgccggt tcactgtttc ccatcgcgcg ctgccagccg cgcctcgacg
     9121 gccgcgcgct cctccgggtc gttgacgttg acgaagttct ccggccggtc ggagaaatcc
     9181 acccgggcga gccggtgctg cgcgaaccag cgatcgatct tgcggtcgcc gccgccgagg
     9241 taggccgcca ggctgtcgcc gagctcgcgc cgcaccagtg cgaacaccgg ctgcaggcgt
     9301 tcgccgtcgt gcggcaccgc gatctcggcg ccctcgtcct cgagcgcgga atatagccgt
     9361 tcggcaaggt cgggggccac cagcggcgaa tcgcagggcg cggtcagcac cagcggcgtc
     9421 gccgcggcgc gcagcccggc cgccagcccg gcgagcgggc cgagaaaacc gctgtcctcg
     9481 tccgtcacca ccggccagcc gaaggccgca tagcgcgcca ggttgcggtt ggcgttgatg
     9541 atcacgcgct cgacttgcgg cgcgagcgcg gccagcacgt gcacgatcat cggccgtccg
     9601 gcgagctcca ccaggccctt gtcctcgccg cccatgcgcg tggcgcggcc gccggcaagg
     9661 atgaccgccg tgatgggtgg actcatgcca gcaggccgcc gccgatgaag cgcgccgcgg
     9721 cggggttggc gggtcccgcg aagaagcgcg ttgcgggggc atgctccagc agccgccccc
     9781 ggtgcaggaa cagcacctcg tcggcgaggc ggcgcgcctg gccgatatcg tgggtggtca
     9841 tgatcacccg cgtgccggcg gcctggaagc cgaggatcag cgattcgatc gcgcgcgtcg
     9901 cctccggatc caggttggcg ctcggctcgt cgagcagcag cacgtccggc ttcagcaccc
     9961 aggcccgcgc gatggccagc cgctgctgct cgccaccaga caaggtgcgg gcggcctgcc
    10021 gggcccggtc ggcgagcccg gcagcctgca gcgcctcgtc caccagcgcc acgcggtccg
    10081 cacgcggcac accgcggacg gccagcgaat gctgcaggtt gccggctacc gagcggcgca
    10141 gcagcacggg gcgctggaac accatgccct ggcggcggcg tgcctggtcc ggcgtgcgcc
    10201 ccgcccagcg gatctcgccg gcggcgggtc gcagcagccc gtggcagagc ttcagcagca
    10261 cgctcttgcc ggcgccgttc ggtcccagca ccacggtgcg cgccgcgtcg cggaccgtga
    10321 acgacacgtc gtcgacgatg gccttgccgc ctgcggcgta gcggagcccg cggacctcca
    10381 gcggcatcag cgggttgccg cccgcgatgc ccgacggtgc cgtcacgggg cggagcgatg
    10441 gatcaggcat ggcggcgatg ggcggcgtct ccgagcaggg tcgcggcggc attgacgccg
    10501 gccgccagga cgatgagcac caggccgagc gcgagggcga gggcgaggtt gcccttgctg
    10561 gtctcgagcg cgatggtggt ggtcatcacg cgggtgtgat gcaggatgtt gccgccgacg
    10621 atcagcaccg cgccgacctc ggcgatcgcc cggccgaaac ccgccagcac cgccgtcagc
    10681 aggcggaagc gcccgtccca cagcagcgtc gccacgctgt cgagccggcc ggcgtcgagc
    10741 gagcgcaggt gttcgtcgta ttcctcgtgc aggtcctgca cggtctggcg ggtgagcgcg
    10801 gcgacgatcg gcaccaccag gatgaactgc gcgatgatca tcgcggtcgg cgtgaacagc
    10861 aggccgaact cgcccagcgg gccggagcgc gacagcagca tgtagaccac caggccgacc
    10921 accaccggcg gcaggcccat caacgcgttg agcaggatga tgacgacgcg ccgtcccggg
    10981 aagcggaaca tgccgatcac ggccccgagc ggcaggccga acagcgccgc caggccgacc
    11041 gcgaagacac tgacgtacag ggacagcagc acgatctcga ccagttccgg gtcgaggccg
    11101 acgacgagtc gcgcggcaag cgcaaaggcg gctgaaaaat cctgcatgtt tccttatgat
    11161 ccggtccgtc cggcacccct gcgcccagcg ccgccttcga acgtgcaagc agggtgccaa
    11221 agcgccaaat ccttgcgcat tttgtcattg tttgcgacat gtttccgctt aatcgatgga
    11281 aattctgccg caacatgaca gtttttcccg tttcagcgcc gatgttgcgg ctttttgacg
    11341 cagcgtatac cctctggcgt cccggctgaa cccgcaggag ttccgcgctt gaagcgtccc
    11401 caaccgagcg agccgctggc gttgcgcaac gcgtctgtgc tgattgtggg cggcgacgcc
    11461 ggctaccgca gccgactgcg cgccgcactc ggtcggcacg tcgccttcgt cgactcggcg
    11521 gatggcgcgg cccgggcggg ccaattgatg gcacgctgcc gtttcggctt cctggtggta
    11581 aatgccgggc tctcagacgc accggcgctc gagtggatcg gcggcctgcg ccaggatggc
    11641 gcggcgccgc gcgtcgtgct ggtcacggaa cgccccgacc gggagctgct gctggaggcg
    11701 atgcgtcgcg gcgtcaacga tgtgctcgag cggcccttcg agatcgaggc ggcacttgcc
    11761 gcgttgcagc gagtcgccgc tattcagccg gccggggatc gagtgccgat gcttggcgag
    11821 ggtcccggcg gcgagttgca gcgtcgggaa ctcatcggtg acagcgccgc gatggccgat
    11881 gtccgcagcc tggtgcggcg tatcgccccc ttgccgggca cggtgctcat cgagggcgag
    11941 accggcaccg gcaaggagct ggtggccagg tggctgcacg cccacagcgc gcgacgcggc
    12001 gccttcgtcg cggtgaactg cggcgccatc gccccggagc tgctggagag cgaactgttc
    12061 ggccatacca agggggcgtt caccagcgcc caccaggtgc gcgagggcct gttcgtcgct
    12121 gcgcgcggcg gcacgctgta cctggacgaa gtcagcgaga tgccgctgca cctcgaggtc
    12181 aagctcctgc gcgtcctcga ggagggcgcc attcgcccgg tgggcgccga ccaggagctc
    12241 gaggtcgatg tgcgcatcgt cgcctccacg cagcatgact tgcgtgccat ggtccgtgag
    12301 cggcgctttc gcgaggatct ctatttccgc ctcaacgtcg cccaggtgcg cctgccgcca
    12361 ttgcgcgaac ggcgtgacga catcgaggtg cttgcgcggc acttcatgga acaactggcc
    12421 ggcggcctcg ggatcgcgcc gctgccgatg gatgcgcagg tgctggcaca gcttcaggag
    12481 catcattggc cgggcaatgt ccgcgagctg aagaacttcg tcgagcgcag cctgatgatg
    12541 ggacgactga tgatcgacga actgaccgcg tccgcggcgg ccgaagcgga tgaggtggcc
    12601 gcctatccgc tcgactggac gctcgaggaa gtcaaggaac accacatggc ccgggtgctc
    12661 cgcgccctgg gcggcaacaa gtctgcggcg gcgcgccgcc tcggaatctc gcgcaagacg
    12721 ctggaacgcc gcgaattgcc ggatatcagt ggccgcaccg cctgaacctg cacgcggcgg
    12781 caggcacgcc atttcaacat cgaaaccaac gccaaggaga cattcatggg cgccatgaaa
    12841 ctggacgaag agaaattgaa cctggagatc cgcaagttcc tcaaggtcgt gggcatcacc
    12901 tcgcagcgcg agatcgaaca ggcggtacgg gaagcagcca ccgccggaac gctgccggcg
    12961 ggaacgggag tgctggccag ggtgacgctc gagctgccgg tactgggagt ccggagggag
    13021 atcacccagg acctcgacct gggctgattc gcacagggct ctcagacggg ccagatcagg
    13081 ttgccggttt ccgcgaactc gtagccgccg aacttctgcg cgacccggag gcactcggtg
    13141 ctgcgcaaaa aatcgagcag tttctggaac agggtgcgaa agaacatacc ccggtgcatg
    13201 gcgaagtcga aggcttccca gcccatgggc atgaagtcca gcccgaactc ggtggcggcg
    13261 gcgcgcacgc cgatggcgac gtccgcgtag cccatcgcga tcatggaggc ggcgtcgcgt
    13321 tccgagaacg cgcgtcccgc cacgcgccgg gccgccgggt cgaggccgtg tcgggcaatg
    13381 atctcgtgca ggaagcgctg ggagccggcg ccctcctggc gcatcaccca gcgcatttcg
    13441 cggctgaaca gctcagtgac gtcggtgccc gacaggccat tggccagcag caggccctgc
    13501 tcgcgcagga aggcgcgcac cagcacccag tcatggtgcg gcgggtgctg ccgcagcaga
    13561 gcggggtgcc gttgcctgct ttcctcggcc gggccccagt ggatgccgca gatgtccgcg
    13621 cgcttgcgtg acaacaacga cagccccagt tgcgtgccgg tcgatgaata gctgatcaac
    13681 gcgcgcgcct ggacttcgtt ggccacgtga atgatggccc ggtacagcaa cgggtcgtcg
    13741 ctgccggcga tcagcatccg atcggccagc aggccgccgt gactcgactc cagcagccac
    13801 tggtccacca ggtcgcgcgg aaacagccac ttcccggtgg ccttggtagc gggaatctcg
    13861 cctgcactga ccagtgcgta gactttcttc tcgttgatct gcaggtaggc ggccacctgg
    13921 cgcacgctca tgaagcgcgg cagcgctgcg gcttcggtcg ggccggcggg ctggctgtcg
    13981 tccatgattt tcgtcggcct cccccacgca agtcgtcaga tggcgatatg caagaagcat
    14041 tccggtcatc ggttagcgcg ccatgccgac ggattccggc cgcggcggaa acgccggccg
    14101 gtgcggtcgg ggacagaatg tctcattgat ccgggccgac gccgcgactg cccggcgagg
    14161 ggtccgtgct ggtaccctag tgagccggat tcgtcccggg actgaccgca ggaacccgat
    14221 cgccatgtca gctacgcccg tcgcgacatt tcccgtcggc attctgatgg cccgcaaggt
    14281 ggtgcgccgc agcggctggt cactgccgag ctgggatgtg cttggcgtgt tctgtggcga
    14341 gcaatttgcg gcgccgtcgc gcacgcgcga gctggtgcac gaggccgacg acctgcagca
    14401 ctttctgtac cgcggcttcc gcatcgatct ctaccgcgat gccgccgaga cctactggtg
    14461 gaacctgacg ggggaacggc cgtcgctgtt cgttctctgt ctgaaggagg acggcgaaga
    14521 actagtgccg aacgtcgtga ccgcagatca cgaggctgcg caggccgcgg tggaaagcaa
    14581 cggcgcggcg ttctctgtcc cgatcccgcc tgaaattcac acagaactgg agaaggtcgt
    14641 gatggaacat taccgcccgg agccgccacg caagcgcaag cagcacaagt ggcacaagga
    14701 ggacaagcat tgagtggggc tgaggaccag cggaaaaacg cggcgggaat ccagccggag
    14761 gagcctttcc tgagccgctg ggcgcgactc aagaaggcct cgcgggaagg tgccgggcag
    14821 gcgccggttc ccgcccccgc agccggcccg gacaaggctg atgtcgaggc gtcgaaccag
    14881 gccgaaccgg catcgaccga gccggaggtc gatgcggcga gcacggcaca gggcggcccc
    14941 gagaccctgg acctgccacc tttggagtcg ctcacccccg agtccgactt ttcggctttc
    15001 atgcgtcccg gggtggaccc cgggctgcgc cgcgaggcgc tgcgcaagct gttccagaac
    15061 cccaagtacg gcttgctcga cgaactcgac ccctatcgtg ctgattacgc agcgtttacc
    15121 ccgctgggcg acatcatcac cgccgatatg aagttccacg ccgagcggct cctgcgcaag
    15181 cagttggagg aagcgctcga ggcggaagag gccgacgact caagtgtggc ggacacgcag
    15241 ctcccggcgg gcgacgaccc ggatgaggtg cccgggagca tcgcagccgc tgatcggtcc
    15301 gagggacgtg atgtcgccgc gggcgcgggt tcaaacaatg atcgagagga tgacgatgag
    15361 caccgaaaca gctgagctgc cgagcacggg acccgccggg cgggcgcgcg cagcggcgct
    15421 ggcgttgccc gatgcgcctg tgtccagccc gacttcacgg gtgacctacc agtcccaggg
    15481 gcgggtcgtg gtcatcgggc cggagactga gtcgttgtcg gctgcgcggc agctcgccga
    15541 tcgcgtgccg gtgacaattc atgttcccgg cgttgagcgg ccggacccgg gaaccgtcga
    15601 gggcatcgtg atcgtccgtg gcggcacgcc cgaggtcgag ggtcgcctgg gccgtttcac
    15661 ggtcaacctc gcaacaccgg agggtgcagt tgcactggcg gaggcggcgg ggctggaaat
    15721 cgagcatttc gacctggtgc tggacctttc gccggagccg ttgctcagcc acgaagtgcc
    15781 gccggtcggc tactatgcgc cgcgcggcga cggccgggcg ctcgccaggg cggtcgccga
    15841 actgccggag atgagcggcg agttcgagaa gcccaagtat ttcaacttca agcccgaatt
    15901 gtgcgcgcac tcgcgcagcg aactggccgg ctgcacgcgc tgcatcgatg cctgccccac
    15961 cctggccatc acgtccaacg acgaggtggt tgccgttgat ccttacttgt gccagggcgc
    16021 ggggagttgc gtggcagcct gcccgagcgg cgcaatgagt tacgccttcc cgacggtcag
    16081 cgacctgttg acgcgggtgc gcggcctgct cgaggccttc cgggcggccg gaggagacgc
    16141 cgcgggcctg gtcctgcacg atggctggtc gcgagcccag gtgctcggcg agatcggggc
    16201 ggcgatgccc gaggacctgc tgccgctgga agtggaggag gtcgggtcgg tcggcatgga
    16261 cgcctggctt gcggcgctgg cttatggcgc gcgcggggtc gtcatatgtg tcaccgacta
    16321 cacgccaaaa cgcatgcgtc gtgaactcga ggcccaggtg caattcgccc ggggcgtgct
    16381 tgccggaatg ggctacccgc cgggcacgtt ggcagtcgcc aacgtcagca aaccggtgga
    16441 ggtggcagcc ggctgggcgg aattgccgga ttcgctgcgt ctgcgcccgg ctcgcttcgt
    16501 ggccccggac gacaagcgcg cggtcctcag gctggcgatc gaacacctgt tccaccaggc
    16561 gccgagcccg cgcaaggtcg cggagctgcc tccgggggcg ccgtttggcg aggtgcgggt
    16621 cgacaaggcc gcgtgcacgc tgtgcatggg ctgcgtcagc gtgtgcccgg cggccgcctt
    16681 gtcggccggt ggcgatttgc cgcagctgaa gttcacggaa tggaactgcg tacagtgcgg
    16741 cctgtgcgag aaggcctgcc cggaggatgc gatccggctg catccccgtt tcctgttcga
    16801 cgctgaacaa cgtcagcagc cgcgggtgct gcacgaggaa gcgccgttct gctgcgtgtc
    16861 ctgcggcaag ccgttcacga cctcgggact gctcgacaag ctgcagcgca agctggaggg
    16921 gcactggatg tttcagaccg aggaggcgcg gcgccggttg caaatgtgtg aacactgtcg
    16981 cgtcaaggac atgttcaggg cgggccacgg gcagggtggt ggcctggccc agtaagggac
    17041 atttttggcc gatccatgga gaatatgtcg taggtacatg ccgaaactcg gcgacctgag
    17101 cgtaatcctg gagattaatt gtcattaatt tacgattatt gatggcccgt ttgttgctgt
    17161 attcaacatt gtgatttgtg acgaggttca tatgacggaa gtcgccgggc ccggtgcggg
    17221 tgccgccaac ggggctgtgc agccacttga tgagccgccc cgcctgaatg catggaacct
    17281 gctgggtcgc ctgttggccg cgccgcccga tgaggcggtg ctggaattgc tggctgggct
    17341 ggacgccgcg caggtcgggg gcgataacct gctgggcgcg gcgtgggaga tgctcgccaa
    17401 ggcggccgcc ggcacttcgc cggccgcgct ggaggaggag taccaggacc tgttcattgg
    17461 cgtcggccgc ggtgagctga tgccctacgg ttcctggtat ctcacgggct tcttgatgga
    17521 gcagccgctg gcgcgccttc gcgacgagct tcgtatgctg ggttttgcgc gccgcgaggg
    17581 cgtttccgag ccggaggatc acgccgcggc tttgtgcgac gtgatggcga tgatcagcgc
    17641 cgccgacgat gcagcggtcc ctgtggaagt ccagtccggc tttttctcgc gtcacatagc
    17701 gccctggatg ggcaggtttt tccgcgacat gcagcaggcg ccgtcagcaa ggttctaccg
    17761 tgccgtcggg cagctgggcg agcagtatgt cgcgcttgaa gggcagtatc tcggtgcgcc
    17821 ggtcaaggtg tccggttccg gacagcgacc gtcggtgggt atggcatgaa tggcgtctcg
    17881 aacggagagt gcaatgagtg gtaaatcaat ggtagatgca actggacggc ggcgggtgct
    17941 gaagaccctg gtcgctgcgg gcggcgtcac ggccctggcg gccgtctcgg ggcgggccgt
    18001 cgcgggtccg ggcaacacct ctgtggaaag gaaggcgccg gtcgcgcagg gttaccgaga
    18061 gaccgaccat atccgcactt actattcgaa ggcccgtttc taggtcctgc gggcctggaa
    18121 acgagcattc cgactcataa cggggagtct cggcgatgaa gctgatcaag aagagtaacg
    18181 tttcgaattc cggtcgaccg gctggtgatg gcatcctggg tgccgccatt gaccggcgca
    18241 ccttcctgcg tcgtaccggc ctcgtgctcg gtggcggggc gctggcaact gcgctgcagc
    18301 cgtccatgat ggtgcgcaat gccgaagctg cgcgggtcgc gtcgagtgca cccatcgagc
    18361 tcaagcgctc ggtctgcggt cactgttcag tgggctgcgg cgtcattgcc gaagtgcaga
    18421 acggcgtctg gaccggccag gagccggact tcgattcgcc gctgaacctg ggcgggcatt
    18481 gcgccaaggg ggcggcggtg cgcgagcatg gccatggcga gcgtcgcatc aagtacccga
    18541 tgaagctggt ggacggcaag tggaagcgca tcagctggga cgacgcgatc aacgagatcg
    18601 gcgacaagat gctcaagatc cgcgaagagt ccggtcctga cgcgctgttt ctcgcgggct
    18661 cctcgaaggc gagcaacgag ggcgcctacc tgcagcgcaa gttcatggcg ttctgggggt
    18721 cgaacaactg cgatcaccag gcccgaatct gtcattcgac gaccgtggcc ggtgtagcca
    18781 acacctgggg ctacggcgcg atgacgaact cgtacaacga catgcagaac agcaagtgcg
    18841 cgttctacat cggctccaac gccg
//
LOCUS       DAOMPG010000012        18411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1326_length_18411_cov_3.135051, whole genome shotgun sequence.
ACCESSION   DAOMPG010000012 DAOMPG010000000
VERSION     DAOMPG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18411
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_18411_cov_3.135051"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLS34_01275"
     CDS             <1..246
                     /locus_tag="PLS34_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86134.1"
                     /translation="AGMLALLRDHLEAMRSCEERHGRALRSLLAGKLERSFRRGAGTT
                     VALFAHLVLDGLELERVRAGVVSRRLLPERAEGRAWA"
     gene            237..2084
                     /locus_tag="PLS34_01280"
     CDS             237..2084
                     /locus_tag="PLS34_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRP86135.1"
                     /translation="MGIRPQAARWFELLTPREELTKALECLAETQAVELQTRSQPEQA
                     LELPGLPQALDEFGEFSRRFGAWWPDGEIPPIEEALEPVSAMQDALARLRDWIADAQP
                     LISEGEELEVQAQKLKELGEALDAPELQLPDLHRLAAAGPVLGARFYRLPPKTELRSL
                     PTAVITQSLLSAEGVPHLLAVGPRVQLEGLDRQMTALKARQVTLPEWLPNDIAAAREE
                     VKLRRDRLLERRREVKAELDALDDRHGLKRAIGTMALMAWFAGHAPSLPVTDHFAWVT
                     GWTSDIDGHEINQALDRGKVRHLLRYREAPAGAECPVVMHNPRWAKPFELFPNLLGTP
                     DSNEADPSRIVALVAPLMFGFMFGDVGQGAVLLAIGLYLRKRVPVFGLLVPYGAMAVV
                     FGFLFGSVFSNEELIPALWMHPIAHPTETMAVALGFGVCILTLGLLLDALGAFWRRRF
                     IFWVATRGGILVAYLGLVGAFIKPALLNVMWLGFAWFIVGSVLMTTHNKLLEIPKALG
                     ELVEAMLQICVNTVSFVRVGAFALAHAGLSAAVVGMASAAGGGLGWLLIMILGNALVI
                     ALEGLIVGIQTTRLLLFEFFIRFLTAEGRRLRPLVGPGSAIRPTGRSST"
     gene            2081..2440
                     /locus_tag="PLS34_01285"
     CDS             2081..2440
                     /locus_tag="PLS34_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRP86136.1"
                     /translation="MRLGMYIVMAMTAVTVCVVLTATYLLFTAGFAGAQELEAAAGAA
                     LDPSVMRWGFISAAASAGLSAAAAGYAVAQVGSAAVGALAEKPELMGRVLVMVGLAEG
                     VAIYGLIVAILILNRLA"
     gene            2442..2738
                     /locus_tag="PLS34_01290"
     CDS             2442..2738
                     /locus_tag="PLS34_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRP86137.1"
                     /translation="MALPVLISDRLTASGFRLAGVRPLLTEPEDAAAVFREALALGGP
                     VMITAALARHVPQAQLAKAIGTADPPVAVIPDVVRRVEPADMAEQVRRALGVET"
     gene            2735..3373
                     /locus_tag="PLS34_01295"
     CDS             2735..3373
                     /locus_tag="PLS34_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86138.1"
                     /translation="MKQTDSIVRMQVDALMELLEQSRSAQCQQARDGAELQAAELLRR
                     ARRAARERVSKAAHEERARLEREVRMVEAEIETEQRRSARRRDSALIAAGRKLLAEAF
                     AARWARAEARREWAACALEEAAGVLLGRDWTLEHPADWPDAERKQAIEQARERFGAAV
                     ATQPADGLSAGLKIRSGGALVDMSIQGLLANERSIEGELLAEFNRAAEGEQS"
     gene            3370..5124
                     /locus_tag="PLS34_01300"
     CDS             3370..5124
                     /locus_tag="PLS34_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRP86139.1"
                     /translation="MSKAVIQWIGGPVLKARVADPFHIHEALAVGEQRLLGEVIQLSG
                     DMITAQVYEDTTGLKPGDPVEGTGLPLSVALGPGILGRIFDGLLRPLGDVTEHWFKPG
                     LAAKRTGSFAFTPSVEKDQQLAAGAIFGEVAGQGPGRACLVPPNVAGRVVSIVAPGEY
                     REDATLLVLEDEHGKRRELSMQHDWPVRRPRPAAARLPADAPMVTGQRILDTLFPVAR
                     GGRAALPGGFGTGKTVLQESLAKWCDADIIIYVGCGERGNEMAEVLDEFPELEDPRTG
                     RPLMERTVIIANTSNMPVAAREASIYTAITVAEYFRDQGLNVALMADSTSRWAEALRE
                     VSGRLGELPGESGYPAYLASRLADFYERAARVKTLCGKEGTVTVIGAVSPPAGDFSEP
                     VTSHTKRYVRSFWALDRSRAQARFYPAIHPLTSYAEDVDTLARWWQSQGNSDWKEHRR
                     RLLTLLEQQAKLERMARIVGKDALPDEQQMTLLAADLMNEGFLRQSSFSDIDRYCSPK
                     RQTAMLGLMMQFIDQAEEAVGRGIAPAKIHAMPILRKLQRLGEEFAEDQVDDKPKVAR
                     EIGDAFAQLEKQEAANAS"
     gene            5114..6583
                     /locus_tag="PLS34_01305"
     CDS             5114..6583
                     /locus_tag="PLS34_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRP86140.1"
                     /translation="MRADVILHGAAHRLEGPLLFARRDADVALNDAVEVIGSDGSARV
                     GRVSAVDEENMVIEVLESTTGLGLDETRIRILHRPLEMAVGPNLPGRVFNSVGQPIDG
                     GPPVPAEQMMRIDGLAINPAARDLPRDFIETSISTIDLMNSLVRGQKLPLFSCGGLPH
                     DRLATQIAQRARLRDAAAGEFCVVFVGIGVPYDVAASFTHAMEESGALAHTVMFLNHA
                     DEPSTQRLLTPRFALTAAEYLAFVEGRHVLVVMTDMTNYCEALREVSASHGEIPSRKG
                     YPGYMYSDLATIFERAGCIRGLPGTLTQLPILTMPGDDIGHPIPDLTGYITEGQIVLG
                     RELDRKGVFPPVEVLPSLSRLMDAGTGKGYTHKDHPALAHQLFASYARANKVRVLASV
                     MGVEGLPENDRKFLEFGDAFETRFVAQDGPRTLEQTMALGWELLRALPPSEMTRLSSA
                     QLAEYIEIEDAGVAPGGAAGAGSAAELAEAEAASEGSGY"
     gene            6587..7234
                     /locus_tag="PLS34_01310"
     CDS             6587..7234
                     /locus_tag="PLS34_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRP86141.1"
                     /translation="MPEAGLSPTRSAFLELKEERQLVREGFEFLDEKRVILAQEMLKR
                     LEAWRKARDEYDALHDVAAGALARALGRHGADGIGIYPTVRMEQAAIHLTEHRFLGVN
                     LLDGEFDAGWKKGTVPNSAGIGDCPQIPPAVNPSPEAERCREAFRALVPLAFEMAVMR
                     TSLERLIREYIRTERRARALENVVLPEIEGSLRFMDEQLEAMDQEEAIRVRNAGR"
     gene            7664..7816
                     /locus_tag="PLS34_01315"
                     /pseudo
     CDS             7664..7816
                     /locus_tag="PLS34_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
     gene            8002..>8427
                     /locus_tag="PLS34_01320"
     CDS             8002..>8427
                     /locus_tag="PLS34_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86142.1"
                     /translation="MGNSSGGEHRLHSHWIAAALLALSLAVAGCSGDDGATGPAGPAG
                     QPGPAGPPGAPGTPGAPGTPAPTGMPTDLAIEITDVEIASPPVVTFTVRNQDGVGYGE
                     LAASQIAWTIAKLVPDGATHTWRSYIRRVRAARPAAVTGW"
     assembly_gap    8428..8437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8438..10431
                     /locus_tag="PLS34_01325"
     CDS             <8438..10431
                     /locus_tag="PLS34_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HRP86143.1"
                     /translation="HIPTACANAAPWRVVSICRSSEPAMASAIQSDSEPGTRGTLEHL
                     GNGEYRYTFDTDIAAVTEPVAVEYEPTLTHRVALQVNAPLPRSNAWLDFVPASPGAAP
                     TITRNIATTESCNSCHLNLGKHGGSARVEVEYCVTCHNPEHTDPNSGESLDMAVMIHK
                     IHRGTALPRVADGERYLVWGFSAAPKEFDRVVYPFERRARHGGAAMQAVACQKCHTDD
                     ADWAAEFKSTPTPDGGNWKNVFSKAACASCHEDVPGLGPAGAPWIDPTKADHTAYTEN
                     DCGFCHRDGGLMPTPEQAHRLDLFERARSMQTLIAGAPAFDAAADTLTYRVRIEDADG
                     VDRTPDATWQISLPYPTVDYRTRGVGAGDDQAPNHTGITVAGLTPDSEGFYTVVQALN
                     ANQVDAMVAAGGSGTLVLRAALADAGDTLYIPNVTTAFAISDATPVPRRDPVLAWEGC
                     RNCHMGLTNHGAGTAYNDNAQTCAVCHNSNWYHEAGGGAMRAWWTDPASPTGGVPRNQ
                     GFMTTIHGVHNNSGFMRTAFANGKTPVVFPAMAPGNAGDRSLRNCLQCHVDGAFELPL
                     PEGIAGPSIGALGDINDVDLHVKLTPAAGACASCHVDYSRVDLTTGMSTDPAWNHFKT
                     MGGGISFGWTWGMPDMQGEACALCHGPGASFDLRPAHGL"
     gene            complement(10516..11265)
                     /locus_tag="PLS34_01330"
     CDS             complement(10516..11265)
                     /locus_tag="PLS34_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86144.1"
                     /translation="MLLFAVTSLSAFVRLSNSADAAAAPMAAEGTAVAIEGEAVLMAR
                     GAHRMAASAALLVVVALMVLSLGPRPYLRREGLLSLGMFALVVFLAVLGRWSSGTVGP
                     GVTLGNLLGGFLLFALCWRLAWPNAPAAAARRPALPALLALLALAVQVALGALSHPAH
                     PWVAIIVAALVLALALGAWRAGRRAAAAGFVALLGLQLLIGIGHTAAGSPPEFVLAHN
                     MAALLLFAAIFRMVGKSGKPGSDPHFFKMGV"
     gene            complement(11309..12190)
                     /locus_tag="PLS34_01335"
     CDS             complement(11309..12190)
                     /locus_tag="PLS34_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRP86145.1"
                     /translation="MSAALPEEKPGYARVRVGPIGLVRALLRLVLLVLVTLGFVLGWA
                     LLALWPWGHAGRVALRRGVSRRWAASICRVLGARIEVLGELPQGPGFLISNHVGYLDI
                     PVLLSLTGCRFVSKHEIADWPVIGFLARVGGTLFVRRGSGGRDAGVTLEGMARALEDG
                     DLVVFFPEGTTSAGERILPFRSGLLSLPARDGHPVWPAAIVYDTRDPDIDARLALAWW
                     GKQSLVPHVWKLVCMRGFTVRIAAADAVEARHRKHLAQALQHRVATMHAGLAGNKPGS
                     DPIFFQKMGSDPGLYGD"
     gene            12302..13750
                     /locus_tag="PLS34_01340"
     CDS             12302..13750
                     /locus_tag="PLS34_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP86146.1"
                     /translation="MGLTNLRVAILAFVVVPCLLATALVGWAGLRGLEEQVNARMQED
                     LELIARTLRLPLSRDLAADRPGLIQETIESTVGMDQVYAVHVYDRHGEQVAGIGRRRA
                     AVPTAEAVELAGQRERQEGSADVGEERLVSVFQPLTSDGGQISGLLQVTRHSGAVQGY
                     LDRIQDMALLAIGAIGLLLTGVIVFGHHFGVGRHVTRLEVGMRRIRDGDLEHRLAVRG
                     AAEFRHLARGINGMLDGITSSQREIERQREIERALRERLHRAEKMAAIGRLAAGIAHE
                     LGSPLSTLYGRAQQLLRRAEPDTPAHTALVDISDNAVRMEKTIRQLMDFGRSNPLNRT
                     SVAVAPLLQRLAADARARCAGAVEVEVACDPQLSVPADALRLEQALGNLLDNAAQAAQ
                     GQVRISVSQDAESVHIHVGDDGPGVPAEVGQLIFDPFFTTKPVGEGTGLGLSVATAAA
                     EDHDGRLALSRDPLGGARFTLTLPRETHDGAQ"
     gene            13737..15155
                     /locus_tag="PLS34_01345"
     CDS             13737..15155
                     /locus_tag="PLS34_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRP86147.1"
                     /translation="MGRNDRHSARILVVEDDGGLRELLRSELEDAGYRLTTAPDVAQA
                     QRSIARDRPELVVSDLRLPGDSGRDLLDHCRGLDAPPAFIMITAFGTVEQAVEALKAG
                     ADDFLTKPLRLDHLRLAVARVLEARELRTQLSRFRSLLADGDFHGMVGRSAPMRRLFD
                     AIRQVAPLNGPVIVSGESGTGKELVARALHAESPRRAQPFVPVNCAAMPPDLMESELF
                     GHAKGAFTGAAQQRAGLFVEADGGTMLLDEIGEMQADTQAKLLRVLEDGCIRPLGADR
                     EINVDVRLIAATNRDLEAEVAAGRFREDLFFRLETFPLSVPPLRERGEDLDLLAARFI
                     TRFAALAERHAPTLSEQALALLRAYPFPGNVRELANALERATAFCDGREIRAAHLPER
                     IRSRRINVAPAGNGPEAPRTAGYDAGMMPLLDGDQPVSLDTLERRYIRWVLDRVEGNK
                     RRAAEMLGIGRRTLYRKLDDGV"
     gene            15313..15702
                     /locus_tag="PLS34_01350"
     CDS             15313..15702
                     /locus_tag="PLS34_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HRP86148.1"
                     /translation="MSNRAKQSVALAAALGAGAALAAAPAMAQEYGQQPPPQQQTTEV
                     SERKINQFVDALAEISTIRQTAAAELEAAADMEEAQKVQQDAQQRMVEAVESAGLSVE
                     EYNHIASLMGSDPELAERINAELERRS"
     gene            complement(15791..16615)
                     /locus_tag="PLS34_01355"
     CDS             complement(15791..16615)
                     /locus_tag="PLS34_01355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRP86149.1"
                     /translation="MFASVARYPVHPELLPPIDRLSGSYRLRFGRSPADLAAVQRLRF
                     AVFNQELGEGLASSWDTGMDADPWDDIFHHIVIEHAPSGEIVGSYRLQTGDMAEHNGG
                     YYSEAEFDLCRMSREQLREAVEIGRACVHAEHCNGRVIHLLWRGLAAYLRWNAKRYLF
                     GCCSLPTLDPSVGLAVWRQLKREGFVSTRYSVAPQQGLDFALRRATPVQLGRNELPPL
                     FQSYLNLGAVVCGPPAVDEEFGTIDFFVLLDLERLPERARETFFRDLPAPVLSRSA"
     gene            complement(16758..16976)
                     /locus_tag="PLS34_01360"
     CDS             complement(16758..16976)
                     /locus_tag="PLS34_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86150.1"
                     /translation="MKKQVSLLLVPVALLGLVLSGCASTQTAGKPTARKYEQDKQYIA
                     AVENATRRAGLTVIWVNPPEKKVESSDN"
     gene            17185..17391
                     /locus_tag="PLS34_01365"
     CDS             17185..17391
                     /locus_tag="PLS34_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRP86151.1"
                     /translation="METVVISPAFEVRIPRRVREALGLAPGQRLRVLQYSGRIELVPY
                     RPAFECPGLFGDVVAADDSVPPAR"
     gene            complement(17434..>18411)
                     /locus_tag="PLS34_01370"
     CDS             complement(17434..>18411)
                     /locus_tag="PLS34_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRP86152.1"
                     /translation="RAVAASAGGAADGGSARGTRRRVDVHDAYVEHLLGYVDAAALAP
                     LKLVVNAGNGGAGLVIDRLEPRLPFGFVKLQHEPDGRFPHGVPNPLLPEHRAATAEAV
                     VARGADFGIAWDGDYDRCFLFDERGRFIEGYYIVGLLAEAFLLKHPGARIVHDPRLTW
                     NTVDIVTRGGGVPVMSKTGHAFIKERMRLEDAVYGGEMSAHHYFRDFAYCDSGMIPWL
                     LVAELVSRAGRPLSALVEERMAAFPASGEINRQLDDPPAVLAAIEARYRDYALGIDHT
                     DGLSMDFGNWRFNLRASNTEPLVRLNVESRGDPVLMEIRTVELLARLGG"
BASE COUNT         2921 a         6523 c         6420 g         2537 t
ORIGIN      
        1 gccggcatgc tcgcgctgct gcgcgaccat ctcgaggcca tgcgcagctg cgaggaacgg
       61 cacggtcgtg ccctgcgcag cctgctggcc ggcaagctgg agcgcagctt ccgccgcggt
      121 gcgggcacga ccgtggcgct gttcgcccac ttggtactgg acggcctgga gctggagcgc
      181 gtgcgcgccg gcgtggtcag ccgccggctg ctgccggaac gcgcggaggg tcgtgcatgg
      241 gcataaggcc gcaggcggcg cgctggttcg aactgctgac gccgcgcgag gaactgacca
      301 aggcactcga atgcctggcc gaaacgcagg ccgttgagct gcagacccgc agccagcccg
      361 agcaggcgct ggagctgccg gggctgccgc aggcgctgga cgaattcggc gagttctcgc
      421 gccgcttcgg cgcctggtgg ccggacggcg aaatcccgcc catcgaggag gccctcgagc
      481 cggtcagcgc catgcaggac gcgctcgccc ggctgcgcga ctggatcgcc gacgcccagc
      541 cgctgatcag cgagggcgag gaactcgaag tgcaggcgca gaagctcaag gagctgggcg
      601 aggcgctgga cgcgcccgag ctgcagctgc ccgacctgca ccgcctcgcg gcggccgggc
      661 ccgtgctcgg cgcgcgcttc tatcgcctgc cgccgaagac cgagctgcgc tcgctgccga
      721 ccgcggtgat cacccagtcg ctgctgagcg ccgagggtgt gccgcacctg ctggcggtcg
      781 ggccgcgcgt gcagctggaa gggctcgatc gccagatgac ggccctcaag gcacgccagg
      841 tcacgctgcc ggagtggctg cccaacgaca tcgccgcggc gcgcgaggaa gtgaagctgc
      901 ggcgcgaccg gctgctggag cggcgtcgcg aggtcaaggc cgaactcgac gcgctggacg
      961 accgccacgg cctcaagcgc gccatcggca ccatggcgct gatggcgtgg ttcgccggcc
     1021 acgcgccctc cttgccggtc accgaccact tcgcctgggt caccggctgg accagcgaca
     1081 tcgacggcca cgagatcaac caggcgctcg accgcggcaa ggtccgccac ctgctgcgct
     1141 acagggaagc gccggccggc gccgagtgcc cggtggtgat gcacaacccg cgctgggcga
     1201 agcccttcga gctgttcccc aacctgctcg gcacgcccga ctccaacgag gccgacccca
     1261 gccgcatcgt cgcgctggtc gcgccgctga tgttcggctt catgttcggc gacgtcggcc
     1321 agggcgcggt gctgctcgcc atcggcctgt acctgcgcaa gcgcgtgccg gtgttcggcc
     1381 tgctggtgcc ctacggcgcc atggcggtcg tgttcggttt cctgttcggc agcgtgttct
     1441 ccaacgagga actgattccg gcgttgtgga tgcaccccat cgcgcacccc accgagacca
     1501 tggcggtggc gctcggcttc ggcgtgtgca tcctcacgct cggcctgctg ctcgacgcgc
     1561 tgggtgcctt ctggcgccgc cgcttcatct tctgggtggc gacgcgcggc ggcatcctgg
     1621 tggcttacct cggcctggtg ggcgctttca tcaagcccgc gctgctgaac gtgatgtggc
     1681 tcggcttcgc ctggttcatc gtcggctcgg tgctgatgac cacgcacaac aagctgctgg
     1741 agatccccaa ggcgcttggc gagctggtcg aggccatgct gcagatctgc gtcaacaccg
     1801 tctccttcgt gcgcgtcggc gccttcgcgc tggcgcacgc cgggctgtcc gcggccgtcg
     1861 tcggcatggc ctcggccgcc ggcggcggtc tcggctggct gctgatcatg atcctgggca
     1921 acgcgctggt gatcgcgctc gaggggctga tcgtgggcat ccagaccacc cgcctgctgc
     1981 tgttcgaatt cttcattcgc ttccttaccg ccgagggtcg ccggctacgt ccgctggtcg
     2041 gcccaggctc cgccatccga ccgaccggaa ggagttccac atgagactcg ggatgtacat
     2101 tgtcatggcc atgacggccg tcaccgtctg cgtcgtgctg accgccacct acctgctgtt
     2161 caccgccggg ttcgccggcg cacaggaact cgaggcggcc gccggcgccg cgctcgaccc
     2221 ctcggtgatg cgctggggct tcatctccgc cgccgcctcg gccggcctgt cggctgcggc
     2281 cgccggctac gccgtcgcgc aggtcggcag cgccgcggtc ggcgcactgg ccgagaagcc
     2341 cgaactgatg ggccgcgtgc tcgtcatggt cggcctcgcg gaaggcgtcg ccatctacgg
     2401 gctgatcgtc gcgatcctga tcctgaaccg gctggcctga catggcgctg ccggtgctca
     2461 tcagcgaccg gctcacggcc tcgggcttcc ggctcgcagg cgtgcgcccg ctgttgaccg
     2521 aaccggaaga tgccgcggcg gtgttccgcg aggcgctggc gctcgggggg ccggtcatga
     2581 tcaccgcggc gctggcgcgg cacgtgccgc aggcgcagct ggcgaaggcc atcggcacgg
     2641 cggatccgcc ggtggcggtg atccccgacg tcgtgcgccg ggtcgagccg gcagacatgg
     2701 ccgagcaggt ccgacgcgcg ctgggagtcg agacatgaaa cagaccgaca gcatcgtccg
     2761 catgcaggtg gacgccctca tggagctgct ggagcaatcc cgcagcgcgc agtgccagca
     2821 ggcccgcgac ggggccgagc tgcaggcggc cgagttgctc aggcgcgcgc gcagggcggc
     2881 gcgcgaacgg gtcagcaagg cggcgcacga ggagcgcgcg cgactggagc gcgaggtgcg
     2941 catggtcgag gcggaaatcg agaccgagca gcggcgcagc gcgcgccggc gcgacagcgc
     3001 gctgatcgcg gcgggccgca agctgctggc cgaggcgttc gcggcccgct gggcgcgggc
     3061 cgaggcgcgg cgggaatggg cggcatgcgc gctggaagag gcggccggcg tgctgctggg
     3121 gcgcgactgg acgctggagc acccggcgga ctggccggac gcggaacgca agcaggcgat
     3181 cgagcaggcc cgcgagcgct ttggcgcggc ggtggcgacg cagcccgcgg acgggctcag
     3241 cgccgggctg aagatccgca gcggcggcgc gctggtggac atgagtatcc aggggctgct
     3301 ggcgaacgag cgcagcatcg aaggcgagct gctggcggaa ttcaaccgcg cggccgaggg
     3361 agagcaatca tgagcaaggc cgtcatccag tggatcggcg ggcccgtgct gaaggcacgc
     3421 gtggcggatc cgttccacat ccacgaggcg ctcgcggtcg gtgagcagcg cctgctgggc
     3481 gaggtgatcc agctctccgg cgacatgatc acggcgcagg tgtacgagga caccaccggg
     3541 ctcaagcccg gcgatccggt cgagggcacg gggctgccgc tgtcggtggc gctcggcccg
     3601 ggcattctcg gccggatttt cgacggcctg ctgcggccgc tgggcgacgt gaccgagcac
     3661 tggttcaagc cgggactcgc cgccaagcgg accggcagct ttgcgttcac gccctcggtg
     3721 gagaaagacc agcagctcgc cgccggcgcg atcttcggcg aggtcgccgg ccagggcccc
     3781 gggcgcgcct gcctggtgcc gccgaacgtc gccggtcgcg tggtgtcgat cgtggcgccg
     3841 ggcgagtatc gcgaggacgc cacgctgctg gtgctggaag acgagcacgg caagcgccgc
     3901 gagctgtcga tgcagcacga ctggccggtg cgccggccgc ggcccgcggc cgcgcgcctg
     3961 ccggcggatg cgccgatggt caccggccag cgcattctcg acaccctgtt tccggtagcg
     4021 cgcggcgggc gcgccgccct gcccggcggc ttcggcaccg gcaagaccgt gctgcaggaa
     4081 agcctcgcca agtggtgcga cgcggacatc atcatctacg tgggctgcgg cgagcgcggc
     4141 aacgagatgg ccgaggtgct ggacgagttt cccgagctcg aggatccgcg taccggccgg
     4201 ccgctgatgg agcgcacggt aatcatcgcc aacacctcga acatgccggt ggcggcgcgc
     4261 gaggcctcga tctacaccgc catcaccgtg gcggagtatt tccgcgacca gggcctgaac
     4321 gtggcgctga tggccgactc caccagccgc tgggcggagg cgctgcgcga agtgtccggg
     4381 cggctcggcg agctgccggg cgaatccggc tatccggcct atctcgcctc gcggctggcg
     4441 gatttctacg agcgcgcggc gcgcgtcaag acgctgtgcg gcaaggaagg cacagtgacg
     4501 gtgatcggcg cggtgagccc gccggccggc gacttctccg agcccgtgac cagccacacc
     4561 aagcgctacg tgcgcagctt ctgggcgctg gaccgcagcc gcgcgcaggc gcgcttctat
     4621 ccggcgatcc acccgctgac cagctacgcg gaggacgtcg acacgctggc gcgctggtgg
     4681 cagtcgcagg gcaactcgga ctggaaggag caccgccgcc ggctgctgac cctgctcgag
     4741 cagcaggcca agctcgagcg catggcgcgc atcgtcggca aggacgcgtt gcccgatgag
     4801 cagcagatga cgctgctcgc cgcagacctc atgaacgagg gcttcctgcg gcagagctcc
     4861 ttctcggata tcgaccgcta ctgctcgccg aagcggcaga cggccatgct ggggctgatg
     4921 atgcagttca tcgaccaggc cgaggaagcg gtcggccgcg gcatcgcgcc ggcgaagatc
     4981 cacgccatgc cgatcctgcg caagttgcag cggctcggcg aggagttcgc cgaggaccag
     5041 gtggacgaca agccgaaggt ggcccgggag atcggggatg ccttcgccca gctcgagaaa
     5101 caggaggccg ccaatgcgag ctgacgtgat cctgcacggt gcggcccacc ggctggaggg
     5161 gccgctgctg ttcgcgcggc gcgatgccga cgtggcgctc aacgacgcgg tcgaggtgat
     5221 cggcagcgac ggcagcgcgc gcgtcgggcg cgtgtcggcg gtggacgagg agaacatggt
     5281 gatcgaggtg ctcgagtcca ccaccgggct cggcctcgac gagacgcgca tccgcatcct
     5341 gcaccggccg ctggaaatgg cggtcggccc caacctgccc gggcgcgtgt tcaacagcgt
     5401 cggccagccg atcgacggcg gcccgccggt gccggccgag cagatgatgc gcatcgacgg
     5461 gctggccatc aatccggcgg cgcgcgatct gccgcgcgat ttcatcgaga catccatctc
     5521 gaccatcgac ctgatgaaca gcctggtgcg cggccagaag ctgccgctgt tctcctgcgg
     5581 cggcctgccg cacgaccgcc tcgccacgca gatcgcgcag cgcgcgcggc tgcgcgatgc
     5641 ggcggccggc gagttctgcg tggtgttcgt cggcatcggc gtgccctacg acgtggcggc
     5701 gagcttcact catgccatgg aggaatccgg cgcgctggcg cacaccgtca tgttcctcaa
     5761 ccacgccgac gagccgtcca cgcagcggct cttgacgccg cgcttcgcgc tcaccgcggc
     5821 cgagtacctg gccttcgtcg agggccgcca cgtgctggtg gtcatgaccg acatgaccaa
     5881 ctactgcgag gcgctgcgcg aggtgtcggc cagccacggc gagatcccga gccgcaaggg
     5941 ctatccgggc tacatgtact ccgacctggc caccatcttc gagcgcgccg gctgcatccg
     6001 cgggctgccc ggcacgctga cgcagctgcc gatcctgacc atgcccggcg acgacatcgg
     6061 ccacccgatc ccggacctga cgggctacat caccgagggc cagatcgtgc tgggccgcga
     6121 gctggaccgc aagggcgtgt tcccgccggt cgaggtgctg ccgagcctgt cgcggctgat
     6181 ggacgcgggc accggcaagg gttacacgca caaggatcac ccggcgctcg cgcaccagct
     6241 gttcgcgtct tatgcgcgcg ccaacaaggt gcgggtgctg gcctcggtga tgggcgtcga
     6301 ggggctgccg gagaacgacc gcaagttcct cgagttcggc gacgccttcg agacccgctt
     6361 cgtcgcccag gacggcccgc gcaccctcga gcagaccatg gcgctgggct gggagctgtt
     6421 gcgcgcgctg ccgccctcgg agatgacgcg cctctccagc gcccagctcg ccgagtacat
     6481 cgagatcgag gacgccggcg tcgcaccggg gggcgcggcc ggcgccggat cggccgcgga
     6541 gctcgccgag gcggaggccg ccagcgaagg ctcggggtac tagccaatgc cagaggccgg
     6601 cctcagcccg acgcgcagcg ccttcctcga gctcaaggag gaacgccagc tcgtccgcga
     6661 aggcttcgag ttcctggacg agaaacgcgt catcctcgcg caggaaatgc tcaagcgact
     6721 ggaggcctgg cgcaaggcgc gcgacgaata cgatgcgctg catgacgtgg ccgcaggcgc
     6781 gctggcgcgc gcgctgggcc gccacggcgc ggacggcatc ggcatctatc ccacggtgcg
     6841 gatggagcag gccgccattc acctgacgga acatcgcttc ctcggcgtca atctgctgga
     6901 cggcgagttc gatgccggct ggaaaaaggg gacagtcccc aattccgccg gaattgggga
     6961 ctgtccccaa atcccaccgg cggtgaatcc cagcccggag gcggagcgct gccgcgaggc
     7021 tttccgcgcg ctggtgccgc tggccttcga aatggcggtg atgcgcacca gcctggagcg
     7081 gctcatccgc gagtacatcc gcacggagcg gcgcgcccgc gcgctcgaga acgtcgtgct
     7141 gcctgagatc gagggctcgc tgcgcttcat ggacgagcag ctcgaggcca tggaccagga
     7201 agaagccatc cgcgtgcgca acgccgggcg ctgacaagac gggggctgac caaaacgggg
     7261 gcagacacca gtttcactgg cgttttggtc tgaccccgtt tctggtctga ccccgtttcg
     7321 aggagttaag ctaggcgcct gccaacggga gcctcctatg ctgaagccag cgctgatcgc
     7381 tttttccgcc gtcgccctcg ccggttgcgg caccgtcatc gatttcgggc ccgaacccgg
     7441 cacccccggg gacgaaggcc actggcacta ccagcccgag ctgcacggcc ccggcgctgc
     7501 cccacggccg gacggcgagg ccccggccgc tgcacaacaa gcgcctgcac ctgcaccggc
     7561 cgcccaaccg gcggcgccgg ccgcagcaac cagcccggca gccccgcagc agccggtgct
     7621 gcctcccccg ccccctgcca ccacctcggc gcccgggccg acctccgaag ccatgttcaa
     7681 cgacgccatc cggcgggcgg tggagtccgg cgacatcgac cgcgccctgc gcctgctgga
     7741 agaggccgag cgcctcggct cgcgcagcgc ccggcccacc ttcatcgagg cggtcggcgg
     7801 gcgcaacggc cagtgaccgc attgcatcat ttcagatgca tttagacttg gactaaatcc
     7861 atatacaaat cagttgcata gatcttcctc tccctgtatt aatgcatctg tgatgcctta
     7921 ataacagacc ggcgcaagta cagccggcga aggcgtcgca ggcgcaagca gtcgcaagtc
     7981 gatctctggg aggggacgca catggggaac tcatccggcg gtgagcaccg ccttcattcg
     8041 cattggattg cggccgcgct gctcgcgctg tcgctggcgg tcgccggatg cagcggcgac
     8101 gacggcgcca ccggccccgc ggggcctgcc ggccagcccg ggccggccgg tccgccggga
     8161 gcgcccggca cacccggcgc accaggcacg cccgcgccca ccggcatgcc caccgacctc
     8221 gccatcgaga tcaccgacgt cgagatcgcc agcccgccgg tggtcacctt cacggtgcgc
     8281 aaccaggacg gcgtgggtta cggcgagctt gcggccagcc agatcgcctg gacgatcgcc
     8341 aagctggtgc cggacggcgc cacgcacacc tggcgcagct acatccggcg cgtgcgcgcg
     8401 gcgcggcccg ccgcggtcac gggctggnnn nnnnnnnccc atataccgac cgcctgcgcg
     8461 aacgcggcgc cctggagggt ggtttccata tgccgctcga gcgagccggc catggcctcg
     8521 gccatccagt cggattccga gcccggcacg cgcggcacgc tggagcacct cggcaacggc
     8581 gagtaccgct ataccttcga caccgatatc gccgccgtga ccgagcccgt ggcggtcgag
     8641 tacgagccga cgctcacgca ccgcgtcgcg ctgcaggtca acgcgccgct gccgcgcagc
     8701 aacgcctggc tcgacttcgt gcccgcctcg cccggggccg cgccgaccat cacccgcaac
     8761 atcgccacca ccgagtcatg caacagctgc cacctgaacc tcggcaagca cggcggctcg
     8821 gcgcgcgtcg aggtcgaata ttgcgtgacc tgccacaacc ccgagcacac tgacccgaac
     8881 agcggcgaga gcctcgacat ggcggtgatg atccacaaga tccatcgcgg cacggcgctg
     8941 ccccgcgtgg cggatggcga acgttacctg gtgtggggtt tcagcgcggc gccgaaggag
     9001 ttcgaccggg tggtgtatcc cttcgagcga cgcgcgcgcc acggcggcgc agccatgcag
     9061 gcggtcgcct gccagaagtg ccacaccgac gatgccgact gggcagccga gttcaagagc
     9121 acgccgacgc ccgacggcgg caactggaag aacgtgttct cgaaggccgc ctgcgcctcc
     9181 tgccacgagg acgtcccggg actcggaccg gccggcgcgc cctggatcga ccccaccaag
     9241 gccgaccaca cggcctacac ggaaaacgac tgcggcttct gccaccgcga cggcgggctg
     9301 atgccgacgc cggagcaggc gcaccgcctc gacctgttcg agcgggcgcg cagcatgcag
     9361 acactcatcg ccggcgcgcc ggcgttcgac gccgcggcgg acacgctcac ctaccgggtg
     9421 cgcatcgagg atgccgatgg cgtcgatcgc acgccggacg ccacctggca gatctcgctg
     9481 ccctacccca cggtcgatta ccgcacccgc ggcgtcggcg cgggcgacga ccaggcaccc
     9541 aaccacacgg gcatcaccgt ggccgggctg acgcccgaca gcgagggctt ctacaccgtc
     9601 gtgcaggcgc tcaacgccaa ccaggtcgat gccatggttg cagcgggcgg ctccggtacg
     9661 ctggtgctgc gcgcggcgct ggcggatgcc ggcgacacgc tgtacatccc caacgtcacg
     9721 accgccttcg cgatcagcga cgcgacgccg gtgccgcggc gcgaccccgt gctggcgtgg
     9781 gaaggctgcc gcaactgcca catgggcctg accaaccacg gcgccggcac ggcgtacaac
     9841 gacaacgccc agacctgcgc cgtatgccac aactcgaact ggtatcacga ggccggtggc
     9901 ggcgcgatgc gcgcctggtg gaccgacccc gcttcaccga ccggcggcgt gccgcgcaac
     9961 cagggcttca tgaccaccat ccacggcgtg cacaacaact cgggcttcat gcggacggcc
    10021 ttcgccaacg gcaaaacgcc ggtcgtcttc ccggccatgg cgccgggcaa tgccggcgac
    10081 cgcagcctgc gcaactgcct gcagtgccac gtggacggcg ccttcgagct gccgttgccg
    10141 gagggcatcg ccggcccgag catcggcgcg ctcggggaca tcaacgacgt cgacctgcac
    10201 gtgaaactga cgccggccgc cggcgcctgc gcctcgtgcc acgtcgacta cagccgggtc
    10261 gacctcacca ccggcatgag tacggacccg gcgtggaacc acttcaagac gatgggaggc
    10321 ggcatctcct tcggttggac ctgggggatg ccggacatgc agggtgaagc ctgcgcgttg
    10381 tgccacgggc cgggcgcgtc cttcgacctc aggcctgcgc acggcctgta agccgctcgc
    10441 cagaggcggc aaggccacgg ggccccgcaa ggggccccgt ttttttggca ggccggtgac
    10501 agggcgaaac cggggtcaga cccccatttt gaaaaaatgg gggtctgacc ccggtttccc
    10561 cgatttgccg accatgcgga agatggcggc gaacaagagc agcgccgcca tgttgtgcgc
    10621 cagcacgaac tcgggcggcg agcccgccgc cgtgtggccg atgccgatga gcagctgcag
    10681 gccgagcagg gccacgaagc cggccgccgc ggcgcgccgg ccagcgcgcc aggcgccgag
    10741 cgccagcgca agcaccagcg ccgcgacgat gatcgcgacc cagggatgcg cgggatgcga
    10801 cagcgcgccg agcgccacct gcaccgcgag cgccagcagc gccagcaggg cgggcagggc
    10861 cggccgccga gccgcggccg ccggcgcatt cggccaggcc agccgccagc acagcgcgaa
    10921 caacaggaaa ccgcccagca ggttgccgag cgtcactccg gggccgaccg tgccggacga
    10981 ccagcggccc agcaccgcca ggaaaaccac cagcgcaaac atgccgagcg acagcagacc
    11041 ctcgcggcgc aggtagggcc gcgggcccag gctcagcacc atgagcgcga ccactaccag
    11101 cagggcggcc gacgccgcca tacgatgcgc gccccgcgcc atcaacaccg cctcgccctc
    11161 gatcgccacc gccgtgccct ccgccgccat cggcgccgcg gccgcatcgg ccgagttcga
    11221 cagtcgcacg aacgcgctca gcgaagtgac tgcgaacagc agcacggcac acagcagcgc
    11281 aaggcgtcgg accaggcggc ggcgggcctc agtctccata caaaccgggg tcagacccca
    11341 ttttctggaa gaaaatgggg tctgaccccg gtttgttgcc ggccagcccg gcatgcatgg
    11401 tcgcgacgcg atgctgcaac gcctgcgcga ggtgcttgcg gtgccgtgcc tcgacggcgt
    11461 cggcggccgc gatgcgcacc gtgaaaccgc gcatgcacac cagcttccag acatgcggca
    11521 ccagcgactg cttgccccac caggcgagcg cgaggcgcgc atcgatgtcc gggtcgcggg
    11581 tgtcgtagac gatcgcggcc ggccacaccg ggtggccgtc gcgtgccggc aggctcagca
    11641 ggccggatcg aaagggcagg atgcgttccc ctgcgctggt ggtgccttct gggaaaaaca
    11701 ccaccaggtc gccgtcttcg agggcgcgcg ccatgccctc cagcgtgacg ccggcatcgc
    11761 gcccgccgct gccgcgtcgt acgaacagcg taccgccgac acgcgccagg aagccgatga
    11821 ccggccagtc ggcgatctcg tgcttggaga cgaagcggca gccggtcagc gacagcagca
    11881 ccgggatgtc gaggtagccg acgtgattgc tgatcaggaa gccggggccc tggggcagtt
    11941 cgccgagcac ctcgatgcgc gcaccgagca cgcggcagat cgaggccgcc cagcgccgcg
    12001 acacgccgcg gcgcagggcc acgcggccgg catggcccca cggccacagt gccagcagcg
    12061 cccagccgag cacgaagccg agcgtcacca gcaccagcag cacgagccgc agcagcgcgc
    12121 gcaccaggcc gatcgggccg acgcgcacgc gggcatagcc gggcttctct tcgggcagcg
    12181 cagcgctcac ggcgggcatc gtgacagcgg gccccggtca ccgcaagctg cgctatgcat
    12241 cgttgcacta ttagcgcatg tgcaataatg acgcatccca gcgtcatcaa gcggtcgtcc
    12301 attgggcctt acgaatctcc gcgtcgccat cctcgccttt gtcgtcgttc cctgcctgct
    12361 ggccacggcc ctggtcggct gggccgggct ccgcggcctc gaggagcaag tcaatgcgcg
    12421 catgcaggag gatctcgagc tgatcgcccg gacgctgcgc ctgccgctca gccgtgatct
    12481 ggctgcggac cggcccgggc tcatccagga gaccatcgaa tccaccgtcg gcatggacca
    12541 ggtctacgcc gtccacgtct acgaccggca cggcgaacag gtcgccggca tcggccgccg
    12601 ccgcgctgcc gtgccgacgg ccgaggccgt cgaactcgcc ggccagcgcg aacgccagga
    12661 gggctccgcc gatgtcggcg aggagcggct cgtctcggtg ttccagccgc tgacctcgga
    12721 cggtggccag atctcggggc tgctccaggt cactcgccac agcggcgccg tgcaaggcta
    12781 tctcgatcgc atccaggaca tggccttgct cgccatcggc gcgatcggtc tgctgctcac
    12841 cggggtgatc gtgttcggcc atcactttgg cgtcgggcgg cacgtgaccc gcctggaggt
    12901 cggcatgcgc cgcatccggg acggcgacct ggagcaccgg ctcgcggtgc gcggcgccgc
    12961 cgaatttcgc cacctggcgc gcggcatcaa cggcatgctg gacggcatta cctcctcgca
    13021 gcgggaaatc gagcggcaac gcgaaatcga acgtgccctg cgcgagcgcc tgcaccgggc
    13081 cgagaagatg gcggccatcg gccggctggc ggccggcatc gcgcacgaac tcggctcgcc
    13141 gttgtcgacc ctctacgggc gcgcccagca gctgctgcgc cgggccgaac ccgacacgcc
    13201 cgcgcacact gcattggtgg acatcagcga caacgccgta cgcatggaga agaccattcg
    13261 ccagctgatg gacttcggcc gcagcaaccc gctcaaccgc acctccgtcg cggtcgcccc
    13321 gctgctgcaa cggctcgcgg ccgacgcgag ggcacgctgc gccggggcgg tcgaggtcga
    13381 ggtcgcatgc gacccgcagc tgagcgtgcc ggccgacgcc ttgcgactcg agcaggcgct
    13441 gggaaacctg ctcgacaatg ccgcccaggc cgcgcaaggc caggtgcgca tcagcgtgtc
    13501 gcaggacgcc gaatcggtgc acatccacgt gggtgatgac ggccccggcg ttcccgcaga
    13561 ggtcggccag ctgatttttg atcccttctt cactaccaag ccggtcggcg agggcaccgg
    13621 cctcggcctc agcgtggcca ccgccgccgc cgaggaccac gatggccggc tggccctgtc
    13681 gcgcgatccg ctcggcggcg cccgcttcac cctgaccctg ccgcgggaga cgcatgatgg
    13741 ggcgcaatga ccggcactcc gcccgcatcc tcgtcgtcga ggacgacgga ggcctgcgcg
    13801 agctgttgcg cagcgaactc gaggacgccg gctaccggct caccaccgcg cccgacgtcg
    13861 cccaagcgca acgctcgatc gctcgggacc ggccggagct ggtggtcagc gacctgcggc
    13921 tgcccggcga cagcggccgg gatctgctgg atcactgccg cggactcgac gccccgccgg
    13981 ccttcatcat gatcacggcc ttcggcaccg tggaacaggc ggtggaggcg ctcaaggcgg
    14041 gcgccgacga tttccttacc aagccgctgc ggctggatca cctgcgcctg gctgtggcgc
    14101 gcgtgctcga agcgcgcgag ctgcgcacgc agctttcccg attccgttcg ctgctcgccg
    14161 acggcgactt tcacggcatg gtcggacgca gcgcgcccat gcggcggctg ttcgacgcca
    14221 tccgccaggt cgcgccgctc aacggcccgg tcatcgtcag cggcgaaagc ggcacgggca
    14281 aggagctggt ggcgcgcgcc ctgcacgccg agagtccgcg ccgggcgcag ccgttcgtgc
    14341 cggtgaactg cgcggcgatg ccgccggacc tgatggaaag cgaactgttc ggccatgcga
    14401 agggcgcgtt caccggcgct gcgcagcagc gcgccgggct gttcgtggag gcggacggcg
    14461 ggaccatgct gctcgacgag atcggcgaga tgcaggccga cacccaggcc aagctgctgc
    14521 gcgtgctgga agacggctgc atccgaccgc tcggtgcgga ccgcgagatc aacgtggacg
    14581 tgcggctcat cgccgcgacc aaccgcgacc tggaggccga ggtcgccgcc ggacgctttc
    14641 gtgaggacct gttcttccgg ctcgagacct ttccattgag cgtgccgccg ctgcgcgaac
    14701 gcggcgaaga cctcgacctg ctcgcggcac gcttcatcac gcggttcgcc gcgctcgccg
    14761 aacgccatgc gccgacgctg tccgagcagg cactcgccct gctgcgcgcc tatccgtttc
    14821 cgggcaacgt ccgcgaactg gcgaacgcgc tggagcgcgc cacggcgttt tgcgacggac
    14881 gggaaatccg tgccgcgcac ctgccggagc gcatccggag ccggcgcatc aacgtggcac
    14941 cggccggcaa cggcccggaa gcgccgcgca cggccggcta cgacgccggc atgatgccgc
    15001 tgctcgatgg tgatcagccg gtgtcgctcg acaccctcga gcggcgctac attcgctggg
    15061 tgctggaccg ggtcgaaggc aacaagcggc gcgcggccga gatgctgggg atcgggcgac
    15121 ggaccctgta tcgaaagctg gacgatgggg tgtgaccagc cggcacatgc gcagcgcagg
    15181 tgtggcaaat tgacacaaac cgtccctcgg gcgcggccgg cgaagcgccg cgctccccgc
    15241 tgttgccggg ctgcagcggg ttggcatggc acttgctcca cttccgacac cccaatcacg
    15301 aatggagcat tcatgagcaa tcgagcgaaa caatccgttg cactcgccgc cgcgcttggc
    15361 gccggcgccg cgctggcggc cgccccggcc atggcgcagg agtacggcca gcagccgccc
    15421 ccgcagcaac agaccaccga ggtcagcgag cggaagatca accagttcgt cgacgcgctg
    15481 gcggagatca gcacgattcg ccagacggcc gccgccgagc tcgaggcggc cgcggacatg
    15541 gaagaagctc agaaggtcca gcaggacgcc cagcagcgca tggtcgaggc cgtcgaaagc
    15601 gcgggcctga gtgtcgagga atacaaccac atcgcatcgc tgatgggttc ggacccggag
    15661 ctcgccgagc gcatcaacgc cgagctcgag cggcgcagct agcgccgctt cgggcgcgga
    15721 cctccgacct aggagctcaa ttccctcccc aagggtaacc ctggcccgcg ccgcacggcg
    15781 cgggcatttt ttatgccgaa cgggacagca cgggcgctgg caggtcgcgg aagaacgtct
    15841 ccctggctcg ctcgggcaac cgctccaggt ccagcagcac gaaaaagtcg atggtgccaa
    15901 actcctcgtc caccgccggc gggccacaga ccactgcacc gaggttgagg tagctctgga
    15961 acagcggcgg cagttcgttg cgcccgagct gcaccggtgt cgcacgacgc aacgcaaagt
    16021 ccagcccttg ctgcggcgcc accgaatagc gcgtgctgac gaagccctcg cgcttcagct
    16081 gccgccagac ggcgaggccc accgagggat cgagcgtcgg cagcgaacag cagccgaaca
    16141 ggtagcgctt cgcgttccag cgcaggtagg cggccagccc gcgccacaac aggtggatca
    16201 cgcgcccgtt gcagtgctcg gcgtgcacgc aggcgcggcc gatttcgacg gcctcgcgca
    16261 gctgctcccg cgacatgcgg cacaggtcga attccgcctc ggagtaatag ccgccgttgt
    16321 gctccgccat gtcgccggtc tgcagccggt agctgccgac gatctcgccg ctcggcgcat
    16381 gctcgatgac gatgtgatgg aagatatcgt cccagggatc ggcgtccatg ccggtgtccc
    16441 agctcgaggc caggccttcg ccgagttcct ggttgaacac cgcgaagcgc agccgttgca
    16501 cggcggcgag gtccgccgga ctgcggccga agcgcaagcg atatgagccg gacagccgat
    16561 cgatgggcgg cagcagttcc ggatggacgg ggtaacgggc gacggacgcg aacatggcaa
    16621 tcccgtgtgg gggcttgccg cgcaagttaa ggtgccttca tgacgctccg gtgacgcgcg
    16681 cgttgccgtt cgcccaaaat aaaaaggacc gcccctcgcc gcagcaaggg tagcggtccc
    16741 gcggcagctc cactccccta gttgtcgctg ctctccactt tcttctcagg cgggttgacc
    16801 cagatgacgg tcagtcccgc acgccgggtc gcattttcga ccgcggcgat gtactgcttg
    16861 tcctgctcgt attttctggc cgtgggttta cccgccgtct gcgtcgaggc gcagcccgac
    16921 aggaccaggc ccagcagggc gaccggaacc agcaacagtg acacttgctt cttcatggcg
    16981 aacctccgac aggctcctct cggaccgccg cgatgccgga tcgggcggca ctggttaaat
    17041 aatagccagt ccggaacact ttgcaagatg cgcaacgcgg cgcatttccg gcccaaatgg
    17101 ctgttttatc tcactacccg actgccacgc cggcacgcag gctggtgcat ggaggggacg
    17161 agagccgagc cagggaggac agcaatggaa acggtggtca tatcgcccgc gttcgaggtg
    17221 cggatcccgc ggcgggtgcg ggaagcgctc ggcctggcgc cggggcagcg cctgcgcgtg
    17281 ctgcagtaca gcggccggat cgagctggtg ccgtaccggc cggcgttcga gtgcccgggg
    17341 ctgttcggtg acgtggtcgc ggccgacgat tccgtgccgc ccgcgcgctg acggtccgcg
    17401 gcgcttcggc gacagcgacg cagcttgccg gtctcaaccg ccgagacgcg ccagcaattc
    17461 caccgtgcgg atctccatca gcacgggatc gccgcggctc tccacgttga gccgcaccag
    17521 cggctcggtg ttcgaagcgc gcaggttgaa gcgccagttg ccgaagtcca tcgacagccc
    17581 gtcggtgtgg tcgatgccga gcgcgtagtc gcggtagcgc gcctcgatgg cggccagtac
    17641 ggccggcgga tcgtcgagct ggcggttgat ctcgcccgag gccgggaacg ccgccatgcg
    17701 ctcctctacc agcgccgaca gcgggcgacc ggcgcgcgat acgagttccg ccaccagcag
    17761 ccagggaatc atgccggagt cgcagtaggc gaaatcgcga aagtaatgat gcgcgctcat
    17821 ctcgccgccg tagacggcgt cctccaggcg catgcgttcc ttgatgaagg cgtgcccggt
    17881 cttggacatc actgggacgc cgccgccccg cgtcacgatg tcgacggtgt tccaggtcag
    17941 ccgcgggtca tgcacgatcc tggcgcccgg gtgcttgagc aggaaggcct cggccagcag
    18001 cccgacgatg tagtagccct cgatgaagcg gccgcgctcg tcgaacagga agcagcggtc
    18061 gtagtcgccg tcccaggcga tgccgaagtc ggcgccgcgc gcgacgacgg cctcggcggt
    18121 cgcggcgcgg tgctccggca gcaggggatt gggcacgccg tgcggaaacc ggccgtccgg
    18181 ctcgtgctgc agcttgacga agccgaacgg caggcgcggc tcgagccggt cgatgaccag
    18241 gccggcgccg ccgttgccgg cgttcaccac cagcttgagc ggcgcgagtg ccgccgcgtc
    18301 gacgtaaccc agcaggtgct cgacgtaggc gtcgtgcacg tcgacccggc ggcgcgtgcc
    18361 gcgggcggac ccgccgtccg ccgcgccgcc ggccgacgcg gcgacggccc g
//
LOCUS       DAOMPG010000013        18407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1327_length_18407_cov_4.016401, whole genome shotgun sequence.
ACCESSION   DAOMPG010000013 DAOMPG010000000
VERSION     DAOMPG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_18407_cov_4.016401"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLS34_01375"
     CDS             complement(<1..496)
                     /locus_tag="PLS34_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP86153.1"
                     /translation="MSAATGVNLRGMRAIYLFEMARFRRTLWQSLATPVITTALYFVV
                     FGSAIGGRIGTIDGVSYAAFIVPGLIMLTVFTESLSNAAFGIYMPKFTGTSYELLSAP
                     ISPLETVLAFVGAAATKSVILGLVILATAGLFVPLELQHPGWMFAYLVLVALAFSLAG
                     FIIGL"
     gene            complement(493..1416)
                     /locus_tag="PLS34_01380"
     CDS             complement(493..1416)
                     /locus_tag="PLS34_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP86154.1"
                     /translation="MQPIIAIRGLAKTYASGLTALKSVDLDIRRGEIFALLGPNGAGK
                     TTLISILCGLVTPSGGSAAVAGHDIMRDYRAARAVIGLVPQELHTDMFETVWDTTCFS
                     RGLFGLKQDKAHIERVLRDLSLWDKRHEKIMTLSGGMKRRVLIAKALAHEPRVLFLDE
                     PTAGVDVELRREMWVLMRRLRDDGVTIILTTHYIAEAEEMADRVGVIHKGELVVVDET
                     RALMSKLGKKQLTLHLQSPLAGIPAGLERWSLELDKDGSELRLVFQAGEEQSCIPALL
                     RRIGELGIEFRDLNTRQSSLEEIFVDLVNER"
     gene            1572..1985
                     /locus_tag="PLS34_01385"
     CDS             1572..1985
                     /locus_tag="PLS34_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP86155.1"
                     /translation="MLIQPYLFFDGRCEEAVAFYQAALGARLEMLMRYRDSPDPLPPG
                     MVPPERIDQVMHASLRIGDAVLMASDDCSGNARHFGGFSLSLSVPDAATADRVFSALA
                     DGGQVVMPLGKTFWSPRFGMVTDRFGVGWMVSQES"
     gene            2001..2354
                     /locus_tag="PLS34_01390"
     CDS             2001..2354
                     /locus_tag="PLS34_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRP86156.1"
                     /translation="MQYMLLIMEPVGQRRARTEQEGRALYDEMLRFSASLKERGLLQL
                     SQSLRSDAEAVRIRSSEGRFSTVDGPFAEAKEMVGGFFLLNCEDRDEAVAIARECPAA
                     RWATVEVRPLGPCFT"
     gene            2392..2832
                     /locus_tag="PLS34_01395"
     CDS             2392..2832
                     /locus_tag="PLS34_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRP86157.1"
                     /translation="MRCMVMVKADRDTEAGVMPSQALLEEMTRFNEQLVRAGVLLAGD
                     GLQPSARGARVIFEGDRRRVVDGPFTESKELVAGYWLWQVRSLEEAIEWAKRCPNPTG
                     ERGELEIRPVYEAADFGDEFTSELQARHARMRTAPGRTGDRDGE"
     gene            2829..4130
                     /locus_tag="PLS34_01400"
     CDS             2829..4130
                     /locus_tag="PLS34_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRP86158.1"
                     /translation="MKPEKVHRTVEAAWRMESARLISGLARMTGDVGLAEELAQDALL
                     AALENWPAGGIPDNPGAWLMAAAKFRAVDHFRRTQRHAEKQEQLGHEIELERLPDGEA
                     EDAALDDPVGDDVLRLIFVACHPVLSMDARVALTLKLLCGLRTDEIARAFLVPEPTMA
                     QRIVRAKRTLQEARVPFEVPRGAELSERLEAVLEVIYLVFNEGYTATSGDAWTRPTLC
                     DEALRLGRMLAELAPREPEAHGLVALMEIQASRLRARVDSRGAPVLLLQQDRSSWDPL
                     LIRRGLAALRRAERLGAVPGPYMLQAAIAACHARARSPDATDWARITALYDALAQVAP
                     SPIVALNRAVAVAMAYGPAAALELVDPLVESKVLRRYHLLPSVRGDLLQKLGRLDEAR
                     NEFQRAAALTGNARERALLLQRAEQCNQAIFAAHEARGDSK"
     gene            4127..4576
                     /locus_tag="PLS34_01405"
     CDS             4127..4576
                     /locus_tag="PLS34_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HRP86159.1"
                     /translation="MSRTTTTKSDEQQVRDLIETWCAAARKSDLDTIMACYTSDVLAF
                     DAIAALQFRGVEAYRKHWEFCIQYMPGGEFILDVHDLQVAVDGSVAFAHYLSHCGCRD
                     AEGNELTGWMRATVCCRRLADGWRIAHEHYSAPFDPESGKALTDLTP"
     gene            complement(4628..5710)
                     /locus_tag="PLS34_01410"
     CDS             complement(4628..5710)
                     /locus_tag="PLS34_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRP86160.1"
                     /translation="MSERLLVATRKGLFTLQRQGAWRITGTAFMGDPVSMVLHDPRNG
                     TLYAALNLGHFGVKLHRSRDGGESWQEIAVPAYPPQPEGAEGPDWSLVQIWCLETGGA
                     DQPGRLWAGTMPGGLFRSDDDGDSWMLVRSLWDRPERLEWFGGGADSPGIHSICVDPH
                     DSRRVTVAISCGGVWQTTDGGDSWQLLGDGLHAVYMPPERAADANIQDPHRVVACPAA
                     PDVFWMQHHNGVFRSTDNAHSWREVAPTKPPRYGFPVVVHPHDPDTAWLVPAAADESR
                     YAPDGAVCVHRTRDGGASFESLRDGLPQEHAYHLMYRHALDIDASGEVLAMGSTTGGL
                     WLSEDSGDHWHTLGLHLPPVYALRFA"
     gene            complement(5745..6014)
                     /locus_tag="PLS34_01415"
     CDS             complement(5745..6014)
                     /locus_tag="PLS34_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRP86161.1"
                     /translation="MPTVTFPPNLRRHLDAAGATVEGASVRAVLEAVFDGRPELRGYL
                     LDDQGMLRRHVAVFVDGRRVRDPRGLSDPVGPTSQVSVVQALSGG"
     gene            complement(6069..7661)
                     /locus_tag="PLS34_01420"
     CDS             complement(6069..7661)
                     /locus_tag="PLS34_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRP86162.1"
                     /translation="MAALPAERLQLRIGALGEDPRVAGREVADRWFLARFYERREFTP
                     AWDDRAKIDQLLTAVSGSAHHGLEPEDYHFGALAELAAASREAPRAGPATELEILATD
                     ALVRLAFHLRFGKVNPERIESTWNFGRDMGSVSPVTALQALLHADDLAAAVEAYAPDG
                     DYYRALMAHLQAYRDIAAQGGWPAVAAGETLRLGMRSPRVPALRALLAVTGDLEAAIY
                     AEEPEIYDQAVEAAVKRFQERHGLDADGAVGRQTLAELNVPVEARIDQLRINLERVRW
                     VFRDLEPRFLLVNIARYRVLLVENQQVVWSTRAVVGRPYRQTPVFKARMTYLEFNPTW
                     TVPPTILREDLLPDIRRDPTVLAQRNMTVLDHQGRRVDPVTIDWSVVTARTFNYMIRQ
                     EPGPENALGRVKFMFPNRYHVYMHDTPARGLFARAARSYSSGCIRLERPFELVDILLA
                     GTQWDRAAIEQVLASGRTRVVNLPQPITVLTLYGTAVPEAGRIHFAPDVYQRDARLLA
                     ALNAPFEFSPPAGYREALDSTG"
     gene            7774..8340
                     /locus_tag="PLS34_01425"
     CDS             7774..8340
                     /locus_tag="PLS34_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRP86163.1"
                     /translation="MCDLCEDELSNHGRARRRLVAAGLGAGALAMMPVAAFANRYPSR
                     ILSFYHTHTGEKLRVAYAEGGRLIREALDEVNHFLRDFRSGEAHTMDPQLLDILHHLQ
                     QSTGGKGPYEIISAYRSPQTNEMLRSNSNGVAQRSLHMEGRAMDVRLRGVDTRRLRQA
                     ALELKAGGVGYYQSSDFIHVDTGRVRYW"
     gene            complement(8351..9010)
                     /locus_tag="PLS34_01430"
     CDS             complement(8351..9010)
                     /locus_tag="PLS34_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRP86164.1"
                     /translation="MSTLLHIDSSPMLDASVTRRLTEYFVAQWRRRVPAGTIIRRDLA
                     ASPPPHPDALTLASASLPAAARSPLQRVTAALADVLLEELEAADVVLIGAPMYNFSVP
                     STLKAWFDLVSRPGRTFRYGPRGHEGLLANKTIIAVTGRGGFYQGPAAPAEATDLQEA
                     LFRHFFAFAGLTDVRFVHAEGQGIDAATAAEHEARARAAIDRLIEDGLGAGAGPAFAA
                     V"
     gene            9141..10523
                     /locus_tag="PLS34_01435"
     CDS             9141..10523
                     /locus_tag="PLS34_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRP86165.1"
                     /translation="MAMQQGDAGVHEAWSSRIVFLMAAVGAAVGLGNLWKFPYTAGVS
                     GGAAFVLVYLVAVSAVAVPIVIAELMIGRRGRMSPPNSFIALAREAGANPGWRFVGWM
                     NLLAVFLILSFYSVVAGWALAYVPKLALGHFTGASAEQVGREFSGLLASPARLALLHA
                     LFMALTVSIVAIGLKRGIERAVRLLMPALLVMLIALVLYAAVAGDLPRALRFLFQADF
                     SKINAQVVLQAIGQAFFSVSVAMGLLITYGAYLDRETRLGSSAVYIAGADTFVALLSG
                     LAIFPLVFANGLDPAEGPGLIFVTLPIAFGQMPAGALFGALFFLLVLVSALTSSIAIL
                     EPMVSWAEEHAWLRRGPAAIVAGAAAWCVGLATVFSFNLWAGWFPLGRFERFRSSTVF
                     DLVDYLTSNLLLPLGGLLIAIFVGWVLSSDTVREELGLGDSRTFRAWRFALRFVAPIG
                     VGWVLLANII"
     gene            10621..11436
                     /locus_tag="PLS34_01440"
     CDS             10621..11436
                     /locus_tag="PLS34_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86166.1"
                     /translation="MSDESEVLERATLEDIYRAAPAEVAEALGIRPFNSGAAFVGLAA
                     GLPMSGIVLNRCIGLGVAAPATEASVREIVTGYAAAGVTRYFVHLHPDSRPVDLAEWL
                     LSAGLVKARGWQKFERPPAPLAAPPTDLEIRAVGREHGEDFARIACHAFDLGEGAVPW
                     LAELPGRPGWHVFMSFAGDVPAGTGAIFVRDGLAWFDFGATAPAFRRRGSQGALLARR
                     IAHAIELGCRKLLTCTGEAVPGDAQHSYRNLLKAGFRETYARGNFTPQPPRPQ"
     gene            complement(11391..>11579)
                     /locus_tag="PLS34_01445"
     CDS             complement(11391..>11579)
                     /locus_tag="PLS34_01445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRP86167.1"
                     /translation="EAEASRNAYQHALTAAGRGRITTVISPAPAFYFAEDYHQQYLAK
                     NPDGYCGLGGCGVKLPRA"
     assembly_gap    11580..11589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11590..12040)
                     /gene="msrA"
                     /locus_tag="PLS34_01450"
     CDS             complement(<11590..12040)
                     /gene="msrA"
                     /locus_tag="PLS34_01450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRP86168.1"
                     /translation="MFASMLEQKSRMITAGEALPGRSTPMPVPEAHFVNGRTMTPPYP
                     DGLEVAEFGLGCFWGAERCFWQEPGVWVTAVGYAGGFTPNPEYREVCSGRTGHAEVVR
                     VVFDPRRVDYQTLLGIFWEVHDPTQGMRQGGDVGTQYRSVIHTHGETQ"
     gene            complement(12179..13168)
                     /locus_tag="PLS34_01455"
     CDS             complement(12179..13168)
                     /locus_tag="PLS34_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRP86169.1"
                     /translation="MDRSERFHKIIRLLKDRRIVSRDAFLEALAISRATFKRDLEYLR
                     DRMDAPILWDAEAGGYRLATEAGEPQNYELPGLWLNAGELYALLAMEQLLDGLQPGLL
                     GPHVRPLRDRIRRLIEVGEHSAEEVGRRIRVLEVGNRPVEPECFQVLASAVLSRRRLR
                     IRHYNRVRGESTERQVSPQRLVHYRDNWYLDAWCHERQALRTFSADAIDDAVLLDRGA
                     REISETKLDRHLGSGFGIFSGARTETAVLQFTPERACWVSRETWHPEQEGQFQLDGSY
                     LLKVPYSDPRELVMDILKYGPDVEVLAPVALAELVVGRLAEARGRYAARRREG"
     gene            13286..14503
                     /locus_tag="PLS34_01460"
     CDS             13286..14503
                     /locus_tag="PLS34_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRP86170.1"
                     /translation="MKLFLVLLAVIALGALWTVFRPRPAPQRRRAPRPAATPTPPRML
                     RGVDEPMKFPQPEARPIPAIAAILEAGAASELEAAEMPELLADLKLLTVEDLSESQRD
                     ELHGRLSTIPLPPRSAQQLMSPDFLAEAATRQFTDLVTREPLLSGKVIGRVNSAFYGL
                     SSPIVSVSHAVTYLGLNAVRNMALQFTLEQAFSSDDPELQAFHARLFDAGTIAAELCS
                     LLGPKIGVRDVGATSTQTVLSFLGDFAMPSLLPDGSAISNWPLGLLERTCNEQLALGV
                     NAAIVGSLLMEEWGLPAAVSDDVRDINRVLVTPAGENMKPRDARLALSYACARIAEGI
                     ALGRIRDPGQIELVGSPEPEYYHLQSYLKVPPLSRLQDMLHAPDARLALSRLVAASQR
                     SRENPQAVLRRRR"
     gene            complement(14546..15292)
                     /locus_tag="PLS34_01465"
     CDS             complement(14546..15292)
                     /locus_tag="PLS34_01465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP86171.1"
                     /translation="MAVIWRRESGGTRYEVRSHGATRRLLSDGVFHSAWNPRTGLTGR
                     TWDLFLPAAFTLAEPPRRILVLGVGAGTALLQMRRFFDPDTLVGVDLNPLHIALGRRF
                     FGLDEAGAALHVADARDWVAGWRGAPFDLVIDDLYGHADGEPLRAVPMNTDWARALSR
                     LVAPRGTLAANFISGRELMACAQLRSRRARAGFASGFRVWGPRDHNAVAVFCRRLTTA
                     ADTRARLRRIAALDDRRSGCVLGYSMRKLW"
     gene            15405..17567
                     /locus_tag="PLS34_01470"
     CDS             15405..17567
                     /locus_tag="PLS34_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP86172.1"
                     /translation="MNNKPTTRTPGRWRWAAAALVLGLLVAALLSGAGDALTLEQLKV
                     RQAELEAWTRANLWLASLLYFAGYVVVVALSIPGAAVMTLAAGALFGLLHGTVLVSFA
                     STFGATLAFLVARFLLREPLRARYGERLRAFDAGIERDGAFYLFSLRLVPLFPFFMIN
                     LLAGLTALRTWTYFWVSQLGMLPGTIVYVYAGTQLARIDSPGDVLSPGLVGAFALVGL
                     MPLLMRWLARWLQARRVYRGHRRPRRFDYNLVVIGAGSAGLVTAYIAAAVKAKVALIE
                     KHRMGGDCLNTGCVPSKALIRSARLVAEAREAGRLGLRDTELEFEFAAVMERVQDVIR
                     QIDPHDSVARYTSLGVEVIEAEARLVSPWEVEAGGRRLAARSIVIATGARPLVPAIPG
                     LEQVDYLTSDTLWQLRELPEKLVVLGGGPIGAELAQCFARFGSEVTIVEMAPRLLPRE
                     DEDAAAEVEARFRAEGMLVATAHRAVRVETGVGGPRLVCEHDGAEVALPFERLLIALG
                     RRANVEGFGLEELGVALSERDTVAVDGLLRTNFPNIYACGDVAGPYQFTHVASHQAWY
                     AAVNALLAPWWSFRADYRVIPWATFTDPEVARVGLSEDEARARGIDCEVTRYGIDDLD
                     RAIADGAARGFVKVLTPPGKDRILGATIVGADAGNLLAEFVLAMKHGLGLNKLLGTIH
                     VYPTMMEANKYAAGNWKRANAPQGALRMAKRVFAWRRG"
     gene            17552..18256
                     /locus_tag="PLS34_01475"
     CDS             17552..18256
                     /locus_tag="PLS34_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRP86173.1"
                     /translation="MAARLKLSVIIPVGPGDRSWRHLLGDLHSLREEAEIILVATPGA
                     IPRDFRMQDYDLEIPTSWLAAPTGRARQQNAGAAAAGGDVLWFLHADSRVTADTLAAA
                     RSFARSAAIGYFDLQFLDDGPRLVRLNALGARIRSRWLRLPFGDQGFILARRVFESLG
                     GFDESLPAGEDHALVWTARRAGVELAPLGAPLLTSARRFAERGWLRTTLRNAFLTVVQ
                     ARRFASARRTAAGERQ"
BASE COUNT         2743 a         6425 c         6411 g         2818 t
ORIGIN      
        1 gcaacccgat gatgaagccc gccaggctga aggccagcgc caccagcacc aggtaggcga
       61 acatccagcc cgggtgctgc aactccagcg gcacgaacag gccggccgtg gcgaggatca
      121 ccagcccgag gatcaccgac ttggtcgccg cggcgccgac gaaggcgagc acggtctcga
      181 gtggtgagat cggtgccgac aacaactcgt agctggtgcc ggtgaacttg ggcatgtaga
      241 tgccgaaggc ggcgttgctc aggctctcgg taaacacggt cagcatgatc agcccgggca
      301 cgatgaaggc ggcgtagctc acgccgtcga tggtgccgat gcgcccgccg atggccgagc
      361 cgaacaccac gaagtacagc gccgtggtga tcaccggcgt cgccaggctc tgccagagcg
      421 tgcgcctgaa gcgcgccatc tcgaacaggt agatggcgcg catgccgcgc aggttgacgc
      481 ccgtcgcggc gctcatcgct cgttcaccag gtccacgaag atttcctcca gcgagctttg
      541 gcgggtgttg aggtcgcgaa actcgatgcc gagctcgccg atgcgcctga gcagggccgg
      601 gatgcacgac tgctcctcgc ccgcctggaa caccagccgc agttcgctgc cgtccttgtc
      661 cagctcgaga ctccagcgct cgagccccgc cggaatgcct gccagcgggg actgcaggtg
      721 cagcgtgagc tgcttcttgc ccagcttgct catcagcgcg cgggtctcgt ccaccaccac
      781 cagctcgccc ttgtggatga cgccgacgcg gtcggccatc tcctcggcct ccgcaatgta
      841 gtgcgtcgtc aggatgatgg tcacgccgtc gtcgcgcagg cgccgcatca gcacccacat
      901 ctcgcgccgc agctcgacgt caacgccggc ggtcggctcg tcgaggaaca gcacgcgcgg
      961 ctcgtgcgcc agcgccttgg cgatcagcac gcggcgcttc atgccgccgg acagggtcat
     1021 gatcttctcg tggcgcttgt cccacagcga caaatctcgc aacacccgct cgatatgcgc
     1081 cttgtcctgc ttgaggccga acaggccgcg gctgaagcaa gtggtgtccc acaccgtctc
     1141 gaacatgtcg gtgtgcagtt cctgcggcac cagcccgatg acggcgcgcg cggcgcggta
     1201 gtcgcgcatg atgtcgtggc cggcgaccgc tgccgagccg ccgctgggcg tgaccagccc
     1261 gcacaggatg ctgatcagcg tcgtcttgcc ggcgccgttc gggccgagca gcgcgaaaat
     1321 ctcgccgcgc cggatgtcga ggtctacact tttgagcgct gtcaggccgg aggcataggt
     1381 cttggccagg ccgcgaatgg cgatgatcgg ctgcatcggc cgaattctag ctgcacggga
     1441 gaccggaatg taatggcgcg gcacagcacg cggcaccccc cgaaaaaatg tccggcccag
     1501 tgtcgaattt gcgccggctc gttcgacgca catgtacgac ccgttgaaaa cagaccgcga
     1561 ggtgactggc gatgctgatc caaccctacc tgttcttcga tggccgctgc gaggaggcgg
     1621 tcgcgttcta ccaggccgcg ctcggggcca ggctcgagat gctgatgcgc taccgggaca
     1681 gcccggaccc gctgccgccc ggcatggtcc cgcccgagcg gatcgaccag gtcatgcacg
     1741 ccagcctgcg catcggcgac gcggtgctca tggcctccga cgactgcagc ggcaatgcac
     1801 ggcattttgg cggcttctcg ctctccttga gcgtgcccga tgccgcgacg gccgaccgcg
     1861 tcttcagtgc gctggccgac ggcggccagg tcgtcatgcc gctcggcaag accttctggt
     1921 cgccgcgttt cggcatggtc acggatcgct tcggggtcgg ctggatggtc agccaggaaa
     1981 gctgaatggc gggggacaag atgcaataca tgctgctgat catggagccc gtcggccagc
     2041 gccgcgcacg cacggagcag gaagggcgcg ccctgtacga tgaaatgctg cgcttcagtg
     2101 catcgctcaa ggaacgcgga ctgctgcagc tgagccagtc gctgcgcagc gatgcggagg
     2161 ccgtgcggat ccggtcgagc gagggcaggt tcagcacggt ggacggcccg tttgcggagg
     2221 caaaggaaat ggtgggcggc ttcttcctgt tgaactgcga ggaccgtgac gaggccgtgg
     2281 cgatcgcccg cgagtgcccg gcggcgcgct gggccacggt cgaggtgcga ccgctcggac
     2341 cctgtttcac ctgagccgcc gcagcggcca caaccgacgg gagcgacttc gatgcgatgc
     2401 atggtgatgg tcaaggccga tcgcgacacc gaggcgggtg tcatgccgag ccaggcgctg
     2461 ctggaggaga tgacccggtt caacgaacaa ctggtgaggg cgggcgtcct gcttgcgggc
     2521 gacgggctgc agccgagcgc gcgcggcgcc cgggtgatct tcgagggcga ccggcgacgc
     2581 gtggtcgatg gccccttcac cgagagcaag gaactggtgg cgggctattg gctgtggcag
     2641 gtgcgctcgc tggaggaagc gatcgagtgg gcgaagcgct gccccaaccc gaccggcgaa
     2701 cgcggcgagc tcgagattcg gcccgtctac gaggccgcgg acttcggcga tgaattcacg
     2761 tccgagctgc aggcgcgtca cgcccgcatg cgcaccgccc cgggccggac cggcgaccgc
     2821 gacggcgaat gaagccggag aaggttcatc gtactgtcga ggccgcctgg cgcatggaat
     2881 ccgcacgact gatctcgggc ctggcccgca tgacgggcga cgtcggactg gccgaggaac
     2941 tcgcccagga cgcattgctc gccgcgctgg agaactggcc ggccggcggc atccccgaca
     3001 atcctggggc gtggctgatg gcggccgcca agtttcgcgc cgtcgaccac ttccgccgca
     3061 cgcagcgtca cgcggagaaa caggagcagc tgggccacga gatcgagctg gagcgactgc
     3121 cggacggcga ggcggaggac gccgcactgg atgatccggt gggcgacgac gtgctgcggc
     3181 tgatcttcgt ggcctgccac ccggtactgt caatggatgc acgcgtcgcg ctcacgctca
     3241 agctgctgtg cggcctgcgc acggacgaga ttgcgcgcgc attcctggtg cccgagccca
     3301 ccatggcgca acgcatcgtg cgcgccaagc gcacgctgca ggaggcgcgg gtgccgttcg
     3361 aggtcccgag gggagctgaa ctctccgagc ggctcgaggc cgtgctggaa gtgatctacc
     3421 tggtgttcaa cgagggctac accgccacca gcggggacgc ctggacgcgt ccgacgctgt
     3481 gcgacgaggc gctcaggctc gggcgcatgc tggcggagct ggcgccacgg gaacccgaag
     3541 cgcacggtct ggtggccttg atggagatcc aggcttcgcg cctgcgcgcc agggtggact
     3601 cacgcggtgc gcccgtcctg ctgctgcagc aggaccgtag cagctgggac ccgttgctga
     3661 tccgccgcgg gctggcggca ttgcgacggg ccgagcgact cggcgctgtg ccggggccct
     3721 acatgctgca ggcggccatc gccgcctgcc atgcgcgcgc ccgcagcccc gacgcgaccg
     3781 actgggcgcg catcaccgcc ctgtacgacg ccctggcaca ggtcgcacct tcaccaatcg
     3841 tggcgctcaa tcgggcggtc gcggtggcga tggcctacgg gcccgcggcc gcgctggagc
     3901 tggtcgaccc gctggtcgag agcaaggtgc tgcgcaggta ccacctcctg cccagcgttc
     3961 gcggcgacct gctgcaaaag ctcggaaggc tcgatgaggc gcgcaatgaa ttccagcgcg
     4021 ccgccgcgct gacgggcaat gcccgcgagc gggccttgtt gctgcagcgt gcagaacagt
     4081 gcaaccaggc gatattcgct gcccatgaag caagaggaga ttcaaaatga gcaggaccac
     4141 tacgacgaaa tccgacgaac agcaggtccg cgacctgatc gaaacctggt gcgcggctgc
     4201 gcgcaagtcc gacctcgaca ccatcatggc ctgctacacg tccgacgtgc tcgccttcga
     4261 cgccattgcc gcgctgcagt ttcgcggcgt ggaggcctat cgcaagcact gggaattctg
     4321 cattcagtac atgccgggcg gcgagttcat cctggacgtg catgacctgc aggtggcggt
     4381 ggacggcagc gtcgctttcg cccactacct gtcgcactgc ggctgccggg acgcagaagg
     4441 caacgagctg accggctgga tgcgggccac ggtctgctgc cggcggctcg cggacggctg
     4501 gcggatcgcg cacgagcact attcggcgcc cttcgatccg gaaagcggca aagcgctgac
     4561 ggacctgacg ccctgaacgg ccgcccggcc ggctgaaccg gtccggtccg ccgctcaccc
     4621 gcggtggtca ggcgaagcgc agcgcgtaga ccggcggcag gtgcagcccg agcgtgtgcc
     4681 agtgatcgcc gctgtcttcc gacagccaca gcccgccggt cgtgctgccc atcgccagca
     4741 cctcgcccga ggcatcgatg tccagcgcat ggcgatacat caggtggtag gcatgctcct
     4801 gcggcaggcc gtcgcgcagt gattcgaagc tggcgccgcc gtcacgggtg cggtgcacgc
     4861 acacggcgcc gtccggggca taacgcgatt cgtcggcggc cgccggcacc agccaggcgg
     4921 tgtccgggtc atgcgggtgt accaccacgg ggaagccgta gcgcggcggt ttggtcggcg
     4981 caacttcccg ccagctgtgt gcgttgtcgg tgctgcggaa gacgccgtta tggtgctgca
     5041 tccagaagac gtccggcgcc gcgggacagg ccaccacgcg gtggggatcc tggatattgg
     5101 cgtccgcggc ccgctccggc ggcatgtaga cggcatgcag gccatcgccg agcagctgcc
     5161 agctgtcgcc accatccgtc gtctgccaaa cgccgccgca ggagatcgcc accgtgacgc
     5221 gccggctgtc gtggggatcg acgcaaatgg aatggatgcc aggcgaatcc gcgccaccgc
     5281 cgaaccactc gaggcgctcc ggccggtccc acagcgaccg caccagcatc caggaatcgc
     5341 cgtcatcgtc cgagcggaac aggccgcccg gcatcgtgcc ggcccacaac cgtccaggct
     5401 ggtccgctcc gccggtctcc aggcaccaga tctgcaccag cgaccagtcg ggcccctcgg
     5461 cgccctccgg ctgcggcgga taggcgggta ccgcgatttc ctgccagctc tcgccgccgt
     5521 cgcgcgagcg gtgcagcttg acgccgaagt ggcccaggtt gagcgccgcg tagagcgtgc
     5581 cgttgcgcgg atcgtgcagc accatcgaca ccgggtcgcc catgaaggcg gtgccggtga
     5641 tgcgccaggc cccctgccgc tgcaacgtga acagcccctt gcgcgtcgcc accagcaatc
     5701 gttcgctcat cggatactcc tcccggagaa acgggccggc catatcagcc gcccgacagc
     5761 gcctggacca cgctcacctg gctggtcggg ccgaccgggt cactcagtcc tcgcgggtcg
     5821 cgcacgcgac ggccgtccac gaacaccgcc acgtggcgcc gcaacatgcc ctggtcgtcc
     5881 agcaggtagc cccgcaactc gggccggccg tcgaacaccg cctccagcac cgcgcgcacg
     5941 ctcgcgccct ctaccgtggc gccggcggca tccaggtggc ggcgcaggtt gggcgggaaa
     6001 gtgacggtcg gcatggtctg gcggcccggg cgtgctcctg gccacgaact atagaccatg
     6061 ccggcgattc agccggtcga gtccagggcc tcccggtagc ccgccggcgg gctgaactcg
     6121 aagggcgcgt tcagcgccgc gagcagccgc gcgtcgcgct ggtagacgtc cggcgcgaag
     6181 tggatgcgtc cggcctccgg caccgccgtg ccgtacaggg tcaacacggt gatcggctgc
     6241 ggcaggttga cgacccgcgt gcggccgctc gccagcacct gctcgatggc cgcccgatcc
     6301 cactgcgtgc cggccagcag gatgtccacc agctcgaagg gccgttcgag gcggatgcaa
     6361 ccggagctgt agctgcgcgc cgcccgcgcg aacagcccgc gcgcgggcgt gtcgtgcatg
     6421 tacacgtggt agcggttggg aaacatgaac ttgacgcggc cgagcgcatt ttccggaccc
     6481 ggctcctggc gaatcatgta gttgaaggtg cgcgcggtga cgaccgacca gtcgatggtg
     6541 acggggtcca cgcgtcggcc ctggtggtcc agcaccgtca tgttgcgctg tgcgagcacg
     6601 gtcgggtcgc ggcgaatatc cggcagcagg tcctcgcgca ggatggtcgg cggtacggtc
     6661 caggtgggat tgaactcgag gtaggtcatg cgggccttga acaccggcgt ctggcggtac
     6721 gggcggccga ccacggcgcg cgtgctccac acgacctgct gattctccac cagcagcacg
     6781 cggtagcggg cgatgttcac cagcaggaag cgcggctcga ggtcccggaa cacccagcgg
     6841 actcgctcca ggttgatgcg cagctggtcg atgcgtgcct cgaccgggac attgagctcc
     6901 gccagcgtct ggcggccgac cgcgccgtcg gcatcgaggc cgtggcgttc ctggaagcgc
     6961 ttcaccgcgg cctccacggc ctggtcgtag atctcgggct cctccgcgta gatcgccgct
     7021 tcgaggtcgc cggtgacggc cagcagggcg cgcagcgccg gcacccgcgg cgaccgcatc
     7081 ccgaggcgca gcgtttcgcc ggccgcgacg gccggccagc cgccctgcgc ggcgatgtcc
     7141 cggtaggcct gcaggtgcgc catcagcgcc cggtagtaat cgccgtccgg cgcgtaggcc
     7201 tccacggcgg cggccaggtc gtccgcgtgc agcagcgcct ggagcgcggt caccgggctg
     7261 acgctgccca tgtcgcggcc gaagttccag gtggactcga tgcgctccgg gttcaccttg
     7321 ccgaagcgaa gatggaaggc cagccggacc agcgcatccg tcgcgaggat ctccagctcg
     7381 gtggccggcc ccgcccgcgg cgcctcgcgg ctggccgccg ccagttccgc cagggccccg
     7441 aagtggtagt cctcgggctc aagtccgtgg tgcgcgctgc cgctaaccgc agtgaggagc
     7501 tggtcaattt ttgcccgatc gtcccaggcc ggggtaaact cccgccgctc gtaaaaacgg
     7561 gccaggaacc aacggtccgc cacctcgcgg ccggcgaccc gcggatcctc gcccagggcg
     7621 ccgatccgca gttgcagccg ctcggcgggc agagcggcca caaggccctc cgactggacg
     7681 ggcccaacga gtgccagcgt cagcatcaca agggtgatgc cattgagaat cagggaccta
     7741 ccggccaatc gggccggcgc cggagagcag cgcatgtgtg acctttgcga agatgaattg
     7801 tccaatcacg gccgcgcacg gcggcgcctg gtcgcagcgg ggctgggcgc cggcgccctg
     7861 gcgatgatgc cggtcgcagc ctttgccaac cgttacccgt cgcgaatcct gagcttctat
     7921 catacgcaca ccggcgagaa gcttcgtgtg gcctatgccg agggcggtcg cctgatccgc
     7981 gaggccctcg acgaggtcaa tcatttcctg cgcgatttcc gcagcggcga ggcgcacacc
     8041 atggacccgc agctgctgga cattctccac cacctgcagc agtccaccgg cggcaagggc
     8101 ccctacgaga tcatctcggc ttatcgttcg ccgcagacca acgagatgct ccgctccaat
     8161 tccaacggtg tggcccaacg cagcctgcac atggagggac gcgccatgga cgtccggctg
     8221 aggggcgtcg atacccgtcg cctgcgccag gcggcgctgg agctcaaggc cggcggcgtc
     8281 ggctactacc agagctccga cttcatccac gtcgacacgg gccgcgtccg ctactggtag
     8341 gcggccggcc tcagaccgcg gcgaaagccg gtcccgcgcc tgcccccagg ccgtcctcga
     8401 tcagccggtc tatggccgcg cgcgcccgcg cctcgtgctc cgcggcggtg gccgcgtcga
     8461 tgccctgccc ctccgcgtgg acgaagcgca cgtccgtcag gcccgcgaaa gcgaaaaagt
     8521 gccggaacag ggcctcctgc aggtccgtgg cctcggccgg cgccgccggt ccctggtaga
     8581 agcccccgcg gccggtcacg gcgataatgg tcttgttcgc cagcaggccc tcgtggcccc
     8641 gcggcccata gcgaaacgtc cgcccgggcc ggctcaccag gtcgaaccag gccttcaggg
     8701 tcgagggcac ggagaagttg tacatcggcg cgccgatcag cacgacgtcg gccgcttcga
     8761 gctcctccag cagcacgtcc gccagcgccg cggtgacccg ttgcagcggc gagcgcgccg
     8821 ccgccggcag cgacgctgat gccagcgtga gcgcgtcggg atggggcggc ggcgaggcgg
     8881 cgaggtcgcg gcgaatgatc gtgcccgccg gcacgcggcg gcgccactgc gccacgaaat
     8941 actcggtcag ccggcgcgtg acgctggcgt ccagcatggg gctggaatcg atgtgcagca
     9001 gtgtgctcaa gtgacgctca cgaggggacg acatccaagg gtaaagcctc cggggccgcg
     9061 atgcagcccc gtattcgcta tcatcacttg cttggagccg gcgcaagacc ggcccggtcc
     9121 gccgcgcggg aggagagcgg atggcaatgc agcaagggga cgccggcgtc cacgaggcct
     9181 ggtcctcgcg aatcgtgttc ctgatggccg cggtcggagc cgccgtcggc ctcggcaatc
     9241 tatggaagtt cccctacacc gcaggcgtca gcggcggcgc ggcgttcgtg ctggtctacc
     9301 tggtcgcggt gagcgcggta gcggtgccga tcgtcatcgc cgaactgatg atcgggcgac
     9361 gcggccgcat gagcccgccg aacagcttca tcgcgctggc gcgggaagcc ggcgccaatc
     9421 cgggctggcg cttcgtcggc tggatgaacc tgctggcggt gttcctgatc ctgtctttct
     9481 acagcgtggt ggccggctgg gcgctggcct acgtgccgaa gctcgcgctg gggcatttca
     9541 ccggcgcctc ggcggagcag gtcggccggg agttcagcgg cctgctggcc tcgccggcac
     9601 gcctggcgct gctgcatgcc ctgttcatgg cgctcaccgt gagcatcgtc gccatcggcc
     9661 tgaagcgcgg catcgagcgg gcggtccgct tgctgatgcc ggcgctgctg gtgatgctga
     9721 tcgcgctggt cctgtacgcg gcggtcgccg gcgacctgcc gcgcgccctg cgtttcctgt
     9781 tccaggcgga cttttcgaag atcaatgccc aggtggtgct gcaggccatc ggccaggcct
     9841 tcttctcggt gagtgtcgcc atggggctgc tgatcaccta tggcgcctac ctcgaccgcg
     9901 aaacccggct cggctcctcg gcagtctaca tcgccggcgc cgacaccttc gtggcgctgc
     9961 tctcgggact ggcgatcttc ccgctggtgt tcgccaacgg cctcgacccc gcggagggcc
    10021 cggggctgat tttcgtgacg ctgccgatcg ccttcggcca gatgccggcc ggcgcgctgt
    10081 tcggcgcgct gttcttcctg ctggtgctgg tgtcggcgct gacctcgtcc atcgccatcc
    10141 tcgaaccgat ggtgtcgtgg gccgaggaac acgcctggct gcgccgcggc ccggcggcga
    10201 tcgtcgccgg tgcggcggcc tggtgcgtcg ggctcgccac cgtgttctct ttcaacctgt
    10261 gggcgggctg gttcccactg gggcgcttcg agcgtttccg cagttcgacc gtcttcgacc
    10321 tggtcgacta cctgacctcc aacctgctgc tgccgctggg cgggctcttg atcgcgatct
    10381 tcgtcggctg ggtgctgtcg tccgacaccg tccgcgagga actgggcctc ggcgacagcc
    10441 ggacattccg cgcctggcgc tttgcgctgc gcttcgtcgc gccgatcggc gtcggctggg
    10501 tgctgctggc gaacatcatt taaccggggt cagagacacg tttccgggcg acagggtccg
    10561 ggcaggcgtt ttcgacggag cagcgagagt cgcacttcat ggaaccgggg aggacggcgc
    10621 atgtctgacg aatccgaggt actggaacgc gccacgctgg aggacatcta ccgggcggcg
    10681 cccgccgagg tggccgaagc cctcggcatc cggcccttca atagtggcgc agccttcgtc
    10741 ggcctggccg ctgggctgcc gatgagcggc atcgtgctga atcgctgcat cggcctcggc
    10801 gtcgccgcgc cggccaccga ggcgtccgtc agggagatcg tcacgggcta cgccgccgcc
    10861 ggcgtgaccc gctacttcgt gcacctgcat ccggacagcc ggccggtgga cctggcggaa
    10921 tggctgttgt ccgccgggct ggtaaaggcc cgcggctggc agaagttcga gcggccgccc
    10981 gcgccgctcg ccgcaccgcc caccgacctg gagatccggg cggtcggccg tgagcacggc
    11041 gaggacttcg cgagaatcgc atgccacgcc ttcgatctcg gcgagggcgc cgtgccgtgg
    11101 ctggcggaac tgccggggcg ccccggatgg cacgtgttca tgagtttcgc cggcgacgtt
    11161 cccgccggca ccggtgcgat cttcgtgcgg gacggcctcg cctggttcga cttcggcgcg
    11221 acggcgccgg cctttcgccg ccgcggcagc cagggcgcgc tgctggcgcg acgcatcgcc
    11281 catgccatcg agctcggttg ccgcaagctg ctcacctgca cgggcgaggc cgtgccgggc
    11341 gacgctcagc actcctaccg gaacctgctc aaggctggtt ttcgcgagac ctacgcgcgc
    11401 ggcaacttca ccccgcagcc cccgaggccg caatagccat cgggattctt cgccaggtac
    11461 tgctggtggt agtcctcggc aaagtagaac gcgggcgccg gcgaaatcac ggtcgtgatg
    11521 cggccgcggc cggcagccgt gagtgcgtgc tggtaggcgt tgcgtgaagc ctccgcctcn
    11581 nnnnnnnnng ctgcgtctcg ccgtgcgtgt gaatcaccga gcgatactgc gtgccgacgt
    11641 cgccaccctg gcgcatgccc tgggtgggat catgcacctc ccagaaaatg cccagcaggg
    11701 tctggtagtc gacccgccgc ggatcgaaca ccacccgcac cacctcggca tgcccggtgc
    11761 gtcccgaaca gacttccctg tattccgggt tgggcgtaaa cccgcccgcg tagcccacgg
    11821 cagtcaccca cacgcccggc tcctgccaga aacaccgctc cgcaccccag aagcagccca
    11881 gcccgaactc cgccacttcc agcccgtccg ggtacggtgg cgtcatcgtc cggccgttga
    11941 cgaaatgcgc ttccggcact ggcatgggcg tggaccggcc gggcagcgcc tcgccggcgg
    12001 tgatcatcct ggacttctgc tcgagcatgg aggcaaacat ctcgttctcc ggaaaattgg
    12061 cagctggaaa ccggggaaac cggggtcaga cacgggtttc gcgaacgaaa cccgtgtctg
    12121 accccggttt ccagccccgg ttttcgacct cattttcgca tcggagtgcc gcagcgattc
    12181 aaccctcgcg gcggcgggcc gcataccgtc ccctggcctc cgccaggcgg cccacgacca
    12241 gctcggcgag cgcgaccggc gcaagcacct cgacgtccgg cccgtacttg aggatgtcca
    12301 tcaccagttc acgcgggtcg gaataaggca ccttcagcag gtaggagcca tccagctgga
    12361 actggccctc ctgctcgggg tgccaggtct cgcgcgacac ccagcaggcg cgctcgggcg
    12421 tgaactgcag cacggcggtc tcggtgcgcg ccccggaaaa gatgccgaag ccggagccca
    12481 ggtggcgatc cagcttggtc tcggagatct cgcgcgcgcc gcgatccagc agcacggcgt
    12541 cgtcgatcgc gtccgccgag aaggtgcgca gggcctgccg ttcgtggcac caggcgtcca
    12601 ggtaccagtt gtcgcggtaa tgcaccaggc gctggggcga cacctgccgc tcggtgctct
    12661 cgccgcgcac gcggttgtaa tggcggatgc gcaggcgccg gcgcgacagc accgccgagg
    12721 ccagcacctg gaagcactcc ggctccaccg gccggttgcc gacctccagc acccggatcc
    12781 gtcgcccgac ttcctcggcg gaatgctcgc cgacctcgat gagccgccgg atccggtcgc
    12841 gcaggggccg cacgtgcggt cccagcaggc ctggctgcag gccgtcgagc agctgctcca
    12901 tcgccagcag cgcatacagc tcgccggcgt tcagccacag cccgggcagc tcgtagttct
    12961 gcggctcgcc ggcttcggtc gccaggcggt agccgccggc ctccgcgtcc cacaggatcg
    13021 gcgcatccat gcggtcgcgc aggtattcca ggtcccgttt gaaagtggcg cgcgaaatgg
    13081 cgagcgcctc gaggaaggca tcccgcgaga cgatccgccg atccttgagc aaccggatga
    13141 tcttgtgaaa gcgctccgac cggtccatgg ctctccgtgc ccgtgcctgt ggccgaatca
    13201 tagcccggcc ggcgttgcgg aacattgcgg cgacgcagca tgcgagattc aagtcggcgc
    13261 ggccaatggc cgataatcag ccggtatgaa gctgtttctc gtgctgctgg cggtgatcgc
    13321 gctcggcgcg ctgtggaccg tattccggcc acggccggcg ccgcagcgtc gccgtgcgcc
    13381 gcgtccggcc gccacgccga caccgccgcg catgctgcgc ggcgtggatg agccgatgaa
    13441 attcccgcag cccgaggcgc ggccgattcc cgccatcgcc gccatactcg aggccggcgc
    13501 cgcctccgag ctcgaagcgg ccgagatgcc ggagctgctc gccgacctca agctgctcac
    13561 cgtcgaggac ctgtcggaat cccagcggga cgagttgcac ggtcggctct cgaccattcc
    13621 gcttccgccc cgctccgcgc agcagctgat gtcgccggat tttctcgccg aggccgcgac
    13681 acgccagttc accgacctgg tcacccgcga gccgctgctg tccggcaagg tgatcggtcg
    13741 cgtgaactcc gctttctacg gcctgtcgtc gccgatcgtc agcgtctctc acgccgtgac
    13801 ctacctcggg ctgaacgcgg tacgcaacat ggcgctgcaa ttcacgctcg agcaggcctt
    13861 ctcgagcgac gacccggagt tgcaggcgtt ccacgcccgc ctgttcgacg ccggcaccat
    13921 tgctgcggaa ctgtgctcgc tgctgggccc caagatcggc gtgcgcgacg tcggcgccac
    13981 ctctacccag acggtgttgt cgttcctcgg cgacttcgcc atgccgagcc tgctgccgga
    14041 cggctccgcg atcagcaact ggccgctcgg gctgctggag cggacctgca atgaacagct
    14101 ggcgctcggc gtgaatgccg ccatcgtcgg cagcctgctg atggaggaat ggggattgcc
    14161 ggccgcggtc agcgacgacg tgcgcgacat caaccgcgtg ctggtcacgc ccgccggcga
    14221 gaacatgaag ccgcgcgacg cccggctggc gctcagctac gcctgcgcgc gcatcgccga
    14281 gggtatcgcg ctcggccgaa ttcgcgaccc gggccagatc gaactggtgg gctcgccgga
    14341 gcccgagtac taccacctgc agagctacct gaaggtgccg ccgctgtccc gcctgcagga
    14401 catgctgcac gcgccggacg cccgccttgc cctgtcgcgc ctggtcgcgg cgtcgcagcg
    14461 ctcacgggag aacccgcagg ccgtactgcg ccgccggcgc tgagccaggc acgttgcggg
    14521 ccggcaacgg ccggccgtca cgcgatcacc agagcttgcg catcgagtag ccgagcacgc
    14581 agccgctgcg ccggtcgtcg agcgccgcga tgcgccgcag ccgggcgcgg gtgtcggcag
    14641 cggtcgtcag gcgtcggcag aacacggcca cggcgttgtg gtcgcgcggc ccccacacgc
    14701 ggaacccgct ggcaaagccg gcgcgggccc ggcgcgagcg cagctgggcg caagccatga
    14761 gctcccggcc cgagatgaaa ttggccgcca gcgtgccccg cggcgccacc agccgcgaca
    14821 gggcgcgggc ccagtcggtg ttcatcggca ccgcccgtag cggctcgccg tccgcgtggc
    14881 cgtagagatc atcgatcacc aggtcgaacg gcgcgccgcg ccagcccgcc acccagtcgc
    14941 gcgcatccgc gacgtgcagc gccgcacccg cctcgtccag cccgaaaaag cgccgtccga
    15001 gcgcgatgtg cagcgggttc aggtccacgc cgacgagcgt gtcgggatcg aagaagcgac
    15061 gcatctgcag cagcgccgtg ccggcgccga cgccgagcac gagaatcctg cgtggcggct
    15121 cggccagggt gaaggcggcc ggcaggaaca ggtcccaggt gcgccccgtc agccccgtgc
    15181 ggggattcca ggcggaatga aacaccccgt cgctcagcag gcggcgggtg gcgccgtgac
    15241 tgcgcacttc gtaacgcgta ccgccggact cacgacgcca gatgactgcc acgcgacgag
    15301 ggtagcatgc ccccgaacag gccggcgcgg acacgcaacg accgccaggc agtaaactcc
    15361 gggctgcgct ccgggtctcc tcgaccaccg ggaggagtac gccgatgaac aacaagccga
    15421 ccacccgcac acccggccgc tggcgctggg cggccgcggc gctcgtgctg gggctgctcg
    15481 tcgccgcgtt gctgtccggg gccggcgacg cgctgacgct ggagcagcta aaggtgcgcc
    15541 aggcggaact ggaggcgtgg acccgcgcca atctctggct ggcgtcgctg ctgtattttg
    15601 ccggctacgt agtcgtggtg gcgctgtcca ttccgggcgc ggccgtcatg accctcgccg
    15661 cgggcgcgtt gttcgggctg ctgcacggca ccgtgctggt gtcgttcgcc agcaccttcg
    15721 gcgccacgct ggcgttcctg gtggcccgct tcctgctgcg cgagccgctg cgggcacgct
    15781 atggcgagcg cctgcgcgcc ttcgatgccg gcatcgagcg cgacggcgcc ttctacctgt
    15841 tctcgctgcg cctggtgccg ctgtttccct ttttcatgat caacctgctg gccgggctga
    15901 cggcgctgcg cacatggacc tacttctggg tcagccagct cggcatgctc cccggcacga
    15961 tcgtctacgt gtacgcgggg acccagctgg cgcgcatcga ttcgcccggc gacgtgctct
    16021 cacccggact ggtcggcgcc ttcgcgctcg tcgggctgat gccgctgctg atgcgctggc
    16081 tggcgcgctg gctgcaggcc cggcgcgtct atcgcggcca ccgcaggccg cgccgcttcg
    16141 actataacct ggtggtgatc ggcgccggct cggcggggct ggtgaccgcc tacatcgcgg
    16201 cggccgtcaa ggcgaaggtg gcgttgatcg agaagcatcg catgggcggc gactgcctca
    16261 acaccggctg cgtgccgtcg aaagcgctga tccgctcggc gcgcctggtg gcggaggcgc
    16321 gcgaggccgg ccgcctcggg ctgcgggaca cggagctgga gttcgagttt gccgccgtca
    16381 tggagcgcgt gcaggatgtg atccgccaga tcgatccgca cgattccgtg gcgcgctaca
    16441 ctagcctcgg ggtcgaggtc atcgaggccg aagcgcggct ggtgtcgccc tgggaggtcg
    16501 aggccggcgg ccgccgcctg gcggcgcgca gcatcgtcat cgccaccggc gcgcgcccgc
    16561 tggtgccggc gattcccggc ctcgagcagg tcgactatct caccagcgac accctctggc
    16621 agttgcgcga actgccggag aagctggtcg tgttgggcgg cgggccgatc ggcgcggaac
    16681 tggcgcagtg cttcgcccgc ttcggcagcg aagtgacgat cgtggagatg gcgccgcgcc
    16741 tgctgccgcg ggaggacgag gacgcggccg cggaggtgga ggcgcgtttc cgcgcggagg
    16801 gcatgcttgt ggccaccgcg caccgggcgg tgcgggtcga gaccggcgtg ggggggcccc
    16861 gcctggtctg cgagcatgac ggcgccgagg tcgccctgcc cttcgagcgg ctgctgatcg
    16921 cgctcggccg gcgcgccaat gtcgaaggct tcgggctcga ggagctcggc gtggcgctca
    16981 gcgagcgcga caccgtcgcg gtggacgggc tgctgcgcac caacttcccg aatatctacg
    17041 cctgtggcga cgtcgccggg ccataccagt tcacccacgt ggcctcgcac caggcctggt
    17101 atgcggcggt caacgcgctg ctggcgccct ggtggtcgtt tcgcgccgac taccgagtca
    17161 tcccgtgggc cacgttcacc gatcccgaag tggcgcgtgt cgggctgtcc gaggacgagg
    17221 cacgcgcccg gggcatcgac tgcgaggtga cgcgctacgg catcgacgat ctcgatcgcg
    17281 ccatcgccga cggcgccgcg cgcggcttcg tcaaggtgct gaccccgccc ggcaaggacc
    17341 ggatcctcgg cgcgaccatc gtcggcgccg atgcgggcaa cctgctggcg gagttcgtgc
    17401 tggcgatgaa gcacggactg ggcctcaaca agctgctcgg cacgattcac gtctacccga
    17461 ccatgatgga ggcgaacaag tacgccgccg gcaactggaa gcgggcgaat gcgccccagg
    17521 gcgcgctgcg catggcaaag cgcgtgttcg cgtggcggcg cggctgaagc tgtcggtgat
    17581 cattccggta gggccgggcg accggtcctg gcggcacctg ctgggcgacc tgcattcgct
    17641 gcgcgaggaa gccgagatca tcctggtggc gacgcccggt gcgattccgc gcgacttcag
    17701 gatgcaggac tacgaccttg aaataccgac cagctggctg gcggccccga ccgggcgcgc
    17761 gcggcagcag aatgccggcg cggcggctgc cggcggcgac gtgctgtggt tcctgcacgc
    17821 cgattcgcgc gtcaccgccg ataccctggc cgccgcccga agcttcgcgc gtagcgccgc
    17881 catcggctac ttcgacctgc agttcctcga cgacggccct cgcctcgtcc gcctgaacgc
    17941 gctcggcgcc cgcatccgca gccgctggct caggctgccc ttcggcgacc agggcttcat
    18001 tctcgcgcgg cgcgtgttcg agtcgctcgg cggcttcgac gagtcgctgc ccgccggcga
    18061 ggaccacgcg ctcgtctgga cggcccgccg ggctggtgtg gaactggcgc cgctgggcgc
    18121 cccgctgctg accagcgcgc gccgtttcgc cgagcgcggc tggctgcgca cgaccttgcg
    18181 aaacgccttc ctgaccgtcg tgcaggcacg gcgcttcgca agtgcgcggc gcacggccgc
    18241 cggcgagcgt cagtgaacgg cggggtggcg atcctggtga agacgcccgg cttgtcgccg
    18301 gtcaagacgc ggctggccgc cggcctgggc gcggcgcgcg ccgcgcgctg gcaccagctg
    18361 gccgcggcgg ccgtcgcgga agcagtggcc agagtggccg gactgag
//
LOCUS       DAOMPG010000014        17811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1397_length_17811_cov_5.138038, whole genome shotgun sequence.
ACCESSION   DAOMPG010000014 DAOMPG010000000
VERSION     DAOMPG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_17811_cov_5.138038"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2783)
                     /locus_tag="PLS34_01480"
     CDS             complement(<1..2783)
                     /locus_tag="PLS34_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP86174.1"
                     /translation="MITWFARNDVAANLLMVTLLALGAWALSARIPLEVFPAFELRTV
                     VVRVPFPGATPAEVEEGITVKIEEAIQDLDGIKQLRSTAVESLGTVVVEVDNRFDPRE
                     LMNDVKARVDAVSTFPADAERPVVYVPEIRRDVITVVISGDLPEHELRLVAERVRDEL
                     VDLEEITQVELDGVRPYEISIEISEQTLRQYGLTLEQVAAAVRRTSLDLAAGAVRTTG
                     GEVLIRTQGQARAGREFEDIVVRGESGTRLTVKDLGVVRDGFSEDPLEFRYNGRSAAF
                     IDVYRVGEQSAIEVARVVRDYIASPPGWLPPGVQLDYWRDQSRVVQARLNTLLKSALQ
                     GGILILVLLTLFLRPAVAFWVVVGIPVAFMAGIALMPVLGVTINLISLFAFILVLGIV
                     VDDAIVTGENIFTRLQKGEDPLQASIKGTQEIAVPVTFGVLTTVVAFMPLLMIEGVRG
                     QIFAQIPLIVIPVLLFSLVESKLILPAHLKHVRIRNGAGDGPLSRLQAAIQRGLERGT
                     QAAYTPVLKASLRNRYLAGAIFGALAIILFSIAIGGHLRFVFFPRIQAETASATLTMP
                     PGTPFDTTAAAIRRINAEAESLRERYRDQDTGESVIKGILASIGSAGGGSTPRTDLGR
                     VVFEIVPPEERTLEVTSSDLVREWRQAIGSIPGAKELSFRAEIGGGGSPLDVQITGPE
                     FGPLRTLAGQVRERLQTYPGVFDITDSFEDGKQEIKLRIRPEAELLGITLDDLARQVR
                     HAFFGFEAQRIQRGREEVRVYVRYPESERRSLDNLATMRIRTPDGAEVPFGEVASAEY
                     GRGFAQIRRVDRNRTINVTADVNKETADIEAIKRDLNDFLAEAVPGYAGVSFTLEGEA
                     REQRESFGSLGYGLMFVLFVIYALLAIPFRSYLQPLIVMSVIPFGAAGAMLGHMIMGM
                     SLTIMSLMGMLALT"
     gene            complement(2789..4021)
                     /locus_tag="PLS34_01485"
     CDS             complement(2789..4021)
                     /locus_tag="PLS34_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP86175.1"
                     /translation="MISKLLKWILPVAVLLAALAGGRYIVSQRPEAPQFQPPQVPVSI
                     DAIRVTPEDYRVVIRSEGTVEPRTESTLIPQVSGQIVAISPSFREGGFFEQGDVLVTL
                     DPRDYQLAIASAEAQVAQAESALEQELAQAKVVADDWRMLGREAPELGLRKPQIAAAR
                     ATLLSAQSQLERAKFDLERTRIRAPYDGQVLEKNVDVGQFVSPGTLLARVYATDYVEI
                     RLPLSNRQLEFVDLPERFRGETAQRGTTGPEAVIKATIGRQEWRWSGRIVRAEGAIDT
                     RTRQLFVVAQVDDPYERGVDGQPPLRIGQFVEAEIAGQVLANAFVLPRSALREATEVL
                     VVDAESRLQRRPVTVAWTDGESAVITAGLEPGEVVNITPLAVAANGTLVAATIDGVPP
                     APRRGPPGDRVAAEGRAQ"
     gene            4194..4886
                     /locus_tag="PLS34_01490"
     CDS             4194..4886
                     /locus_tag="PLS34_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP86176.1"
                     /translation="MPHRKAPCLGAFWVGRVNCERCSASAPAPFSVLDAAEHARPLAQ
                     VENYRAPAGTVIVSADDPAEAVFTIREGFVKLWRRTDKGNHRILRLLRPGEMIGLEAL
                     GQRRYQLSAAALSDVKLCRIPVEILDQLRRNAPELLAELDERWLVQQTRTDELLANIA
                     TGPAPERVLKLLRFLDKLASPDPCPRISRLDMASMLDISSETAARVIADLKKAGRLEE
                     RPDRLEFDPDRL"
     gene            5105..7756
                     /locus_tag="PLS34_01495"
     CDS             5105..7756
                     /locus_tag="PLS34_01495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRP86177.1"
                     /translation="MKRSTLTLLTAICAAFSIQAATAQSVNGDVLEFQPADEPSVVSD
                     GVSTNKVKILRTPDGTLFAVYGDEQDVNINGNSMAWNAKAHAPQKPTDIVIKYSIDNG
                     ATWSAALNIDNTAALSSARGILVQEGEPMLDPATGTPDLVNDPRAVDYPGDSDKPNVY
                     NIGNNIIVTWNGKYCAGDEQRFVVYPELFGVTVPFSCQYASRLVWNSTTKAFNTYPAW
                     GGLPYKTEQLTTGIRDAKQDANRGNMYAYVLNWQEDPLGLKLGEAEGPGSGASGANVN
                     NGTDVWYSHIELLNAAGTGVDPAKFVNSSWSPPTRITRNIFATQPLEGRDIERHPAGD
                     YNRGRVGASRPNIGQIDDHVIIAYEETKGSEGWEDGKYTRYHAFRFDTPPEGGEHGCI
                     ISDPWENSRRVRFLVQSASNEVPLLFIYKQGDFTQGGESDIMLRRAVGGFMPEHLQPP
                     VDVENCRVSIMDDLDPLYSIRLEEHGPAMNLSGSREIGGVPGTGRMGESIDTGANMLE
                     NALAHRGMMRGDLMLIGFSYVPNLAQFQNLDSEAPYTFMVRRSTDAGLTWSDAINLTP
                     EVTAESGYTVKEPRIVPTPGSGPRCATDPSDCQDPNVIYIAYGMQDNVFAYQEAGDVD
                     IYMMVTFDSGASFSAPKAITAGDALYGVADDFEDFETQIKLRPDGRASYTVWSGNDGE
                     FKHAMFRSGLVRGDTRELLTSGGAKSRLTVVPDARSDAVLTETSWKSLADYPAPPADY
                     DFPYGVLDFTVTRVALGGSIEVTLSFPEPLPEGTSYWKFGSTPAAPEAHWYEIPAVIE
                     GSTITYTLTDGLTGDDDLAVNGVIVDPAAPAVYNPTVTPPPPAPTPSSSSGGCTIGNG
                     DKVDPTLLLLASLSLLYLVRRRRMPVC"
     gene            complement(7850..8296)
                     /gene="mrtJ"
                     /locus_tag="PLS34_01500"
     CDS             complement(7850..8296)
                     /gene="mrtJ"
                     /locus_tag="PLS34_01500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF033192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JDVT-CTERM system glutamic-type intramembrane
                     protease"
                     /protein_id="HRP86178.1"
                     /translation="MITLQAATRPWRDPWFFFALAAGPAAWALLALWLPLAEPGWPAV
                     QPGRFLVLAVLYPVLEELVFRGWLQGALLNRGWGRRIAGPVSAANLAAALGFATAHLL
                     RTSPAWAAAVILPGLVFGGFRERHGVGAAIALHMFYNAGFFWLFGA"
     gene            8417..9253
                     /locus_tag="PLS34_01505"
     CDS             8417..9253
                     /locus_tag="PLS34_01505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRP86179.1"
                     /translation="MNLELSEAAIRLGAFAALLGLLLFAQWRWPARGDGRPARRQLVN
                     LGMVAIGTGLLRVGFPVLAVAWAVQVHGGGLFGLLDWPAWLAIPAAVLLLDVAIYWQH
                     RLMHAWPLLWRLHRVHHCDTAFDVTTGVRFHPFELVLSMGIKLALISLLGPHPAAVLA
                     FEVLLSLGALFTHTDIALPRALDRRLRLLFVTPSMHRIHHSTRRIETDSNYGFHLSVW
                     DRLFGSYTREPVEDERRMPIGLEEWREPRDQGLVSLLLNPFRAPGPSHGGNGPDTSER
                     HA"
     gene            9246..10205
                     /gene="arsS"
                     /locus_tag="PLS34_01510"
     CDS             9246..10205
                     /gene="arsS"
                     /locus_tag="PLS34_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637640.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HRP86180.1"
                     /translation="MRDTLPLLVDTDFPRIRRDRLETLQLNLGYLCNIACVHCHVAAG
                     PTRKELMDRATMELALAAAERHGVGTLDLTGGSPEMNPEFRWLVGEARARGLHVMDRL
                     NPTIMREPGYEWVGEFLASHQVEVIASLPCYSRENVDAQRGNGVFEASISALQELNAR
                     GYGRPDSGLVLNLVYNPLGPSLPPPQQALEADYKRLLGEEFDIVFNALFTITNMPIKR
                     FGAVLLAKGQYDGYMETLRNAHRAANLKNVMCRSLVSVDYRGFLYDCDFNQMLGMPLG
                     GAESGPHLRDLLSADESPRGIAVGGHCYGCTAGQGSSCGGALA"
     gene            complement(10202..10780)
                     /locus_tag="PLS34_01515"
     CDS             complement(10202..10780)
                     /locus_tag="PLS34_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86181.1"
                     /translation="MAFNRNHARPLCTDSEYQLFTASLADEIGKHTPVQLRGKIQRAR
                     KLRDKYRDLLKRQRLANRARTGTKKGDRAQTNVRTADKAKLFDEALGRFEAKAAKHAA
                     AEQREAKRAAAKAALATRIADKKKAGARRAARLGKGKARSRKAAGATGYAAESARVSA
                     LGKLARDTRAGARKGQKTAAGKRSQARRDSRR"
     gene            complement(10799..11926)
                     /locus_tag="PLS34_01520"
     CDS             complement(10799..11926)
                     /locus_tag="PLS34_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86182.1"
                     /translation="MNKGHVTTGITALAALLLAMPVTTKAEDEGINFGGAVRLNYGWR
                     DYDDQNKDRLGYFELELFRINARGTVGDVILDAEWRRYNDFQAIHHAWVGYRFDEDRH
                     VELGITQVPFGILPYASHSFWFGGTYYMGFEDDYDTGVKFVQKSGDWTFHYAFFKNPE
                     YVDDGRYGRYSFDLVTGGDQQNSETNQLNFRAARLLQHGNERSTEIGLSLEAGQIYNR
                     ATERDGDRWALGAHVNGNYGPWNLQLQGIRYEFKPENQAGVSADFVQLGAFDFPFLMA
                     ARGNVLSANIARSFEVEWGPITGLNCYNNFTYIDPDVDNSAESIQNVTGCSVSAGGVF
                     AYFDWIAGRNMWFAGGDGIGLDGASAGKWRSRLNINVGYYF"
     gene            12123..13472
                     /locus_tag="PLS34_01525"
     CDS             12123..13472
                     /locus_tag="PLS34_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP86183.1"
                     /translation="MNVTHARIPTLMLVLFGLVNLPLSMLMSPTAAILPNFYLEYSAV
                     TLAGLATATFVARLFDAVTDPLIGYLSDRSGSRKPWMILGTGVVAAGTWFLFNPASDA
                     GIGHLLAWYLFVTLGWTLVEIPHTAMAAELSTGYHERSRIALWRQLLGFAGGILFMAS
                     PMLLIGSATEFSPAVMRKLALFIMLALPLFVALMCLKVPEPARRVRARPVQPADLLRA
                     LRDTRPLQYFLLTQVLFGLATGAVASLFVIYTRWHLGLPDKVSHIAVPMTLAMALGMP
                     LWLQVMKHVDKHRAWAMAAVGMIVTLLLVPWIEPGPGALKPMTGVMASFGFFLGLSSI
                     ALPSLLADIVDYDIWRNRRDRAAIFFSFQALVTKLNQGIGGAIALAIPASFGFSTEAP
                     LSQQAVLGLKIAFVAWPCLLLLPMVVLAWRYPLGRRAHTTLARRIDRRGERRILRGS"
     gene            complement(13882..14511)
                     /locus_tag="PLS34_01530"
     CDS             complement(13882..14511)
                     /locus_tag="PLS34_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86184.1"
                     /translation="MTLRRRVLHWVAEHDDSWLFIVLYVGLGLVLSMTISLFWLAAVV
                     AAHGVLEWVAMGRQGIRHNRLGRVLWHLKLDIGLTLVALWLGLYIEVLFGLAGLGAAA
                     RAGAQTAARTIAWQRAIRGALMTADEAAHVVKAVATRNGAKRPAQPADEVPDDGPPWR
                     RPWSRGDKFAVVFTTTWAVLILLTPVLTDHTVGSALAVLAADLHPWPGR"
     gene            complement(14657..16372)
                     /locus_tag="PLS34_01535"
     CDS             complement(14657..16372)
                     /locus_tag="PLS34_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HRP86185.1"
                     /translation="MAAKTVDLLLLHPPSVYDFREKSILYGPVSDMVPSSVVFEIYPI
                     GFLTIAGYLKQRGLQVRIVNLGLRMLTDPDFDVRKFLAGVRPKAIGIDLHWMPHCHGA
                     IEIARLAKQVHPGVPVLFGGLSATYFHEELLQYPEIDFVLRGDSTEPPLHQLLLALRD
                     GGPLDGIPNLTWRDGDEVRVNPLGFVPPTLDYIDVHPELLIEMAIRYRDFASVLPFNG
                     WLKNPTTMVLPLKGCAFECVTCGSSNSTCQMMTGRRKPVFRSPANLAANLAAISRFSR
                     GPIVIPGDLLQGGRKYASRCIDEIAAAGVGNEICFEFFDVPPIDFLRYIDGKLPNWSF
                     EVSPESHDKTVRHAMEGQAGYENEAMETMLREALKLNCHRIDVFYMIGLPQQTYQSVM
                     ETVDYCEQLFRWGDKRLQCFISPMGPFLDPGSQIFEHPERFGYRKLAHTLEEHRRLLE
                     QPSWEHILNYETEWMTRAQLVDATYDAAERLNELKLRYGRISAKQARAVADGIAQARR
                     LRARVNAGMDGEMDPAAAEQLRGEIASYSQNTVCDKQELFWQRHMLSFKPAGIARVAL
                     NYLRS"
     gene            complement(16468..17667)
                     /locus_tag="PLS34_01540"
     CDS             complement(16468..17667)
                     /locus_tag="PLS34_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP86186.1"
                     /translation="MPAGRFKGIFVVTAVVALAIAWGFWPRPVLIETGEVARRPLQVT
                     VEEEGRTRVVDRFVLYAPVAGYLRRVELEVGDAVALGQALAVLDPLRPGVLDARARAE
                     AEARVAAGRAGLERSRAVLRQAAAEAELAAEEFRRREEMLARQLISRSEFDQARSRMN
                     AMAAAQASAASAVEVARFELEAALAALRHSGASEAEAPAETVPVRSPVAGRVLKVLQE
                     SAGVVSAGQALLEVGDPRRLEVEVEVLSRDAVRIEPGGRVLFERWGGNETLEGVVRTV
                     EPTGFTKISALGVEEQRVLAIADISSPPEAWQRLGDGYRVEARFIVWERPDALTVPAS
                     ALFRRDDGWAVFVVEGDRARLRPVRLGAGSGLLSEVLEGLGEGDAVIVHPDDAVDDGV
                     RVRPFRD"
BASE COUNT         2869 a         6210 c         5927 g         2805 t
ORIGIN      
        1 gtcagcgcca gcatgcccat caggctcatg atggtgaggc tcatgcccat gatcatgtgg
       61 ccgagcatgg cgccggccgc gccgaaggga atcacgctca tgacgatgag cggctggagg
      121 tacgagcgga acgggatcgc cagcagggca tagatcacga acagcacgaa catcaggccg
      181 tagccgaggc tgccgaagga ctcgcgctgt tcgcgcgcct cgccctcgag cgtgaagctg
      241 acgccggcgt agcccggcac cgcctcggcc aggaaatcgt tgaggtcgcg cttgatggcc
      301 tcgatgtcgg cggtctcctt gttgacgtcc gcggtgacgt tgatggtgcg gttgcgatcc
      361 acgcggcgga tctgcgcgaa gccgcgcccg tactcggcgc tggccacctc gccgaacggc
      421 acttccgctc cgtccggggt gcggatgcgc atggtggcca ggttgtccag cgagcggcgc
      481 tcgctctccg ggtagcgcac gtagacccgc acctcttcgc ggccgcgctg gatgcgctgc
      541 gcctcgaaac cgaaaaaggc gtgccgcacc tggcgcgcga gatcgtcgag cgtgatgccg
      601 agcagctccg cttccggccg gatgcgcagc ttgatctcct gcttgccgtc ctcgaagctg
      661 tcggtgatgt cgaacacccc ggggtaggtc tgcagccgct cccgcacctg gccggcgagg
      721 gtgcgcaagg ggccgaattc ggggccggtg atctgcacgt ccaacggcga gccgccgccg
      781 ccgatctcgg cgcggaagct cagctccttg gcgcctggaa tggacccgat cgcctggcgc
      841 cattcgcgca ccaggtcgga gctggtcacc tcgagggtgc gctcctccgg cggcacgatt
      901 tcgaacacca cgcggccgag gtcggtgcgc ggcgtgctgc cgccgccggc cgagccgatc
      961 gaggccagga tgcccttgat gacgctctcg ccggtgtcct gatcccggta gcgctcgcgc
     1021 agggactcgg cctcggcgtt gatgcgccgg atggcggcgg ccgtggtgtc gaacggcgtg
     1081 cccggcggca tggtcagcgt ggccgaggcc gtctccgcct ggatgcgcgg gaagaacacg
     1141 aagcgcaggt ggccgccgat cgcgatgctg aaaaggatga tggcgagcgc cccgaaaatg
     1201 gcgccggcca ggtagcggtt gcgcagcgag gccttcagca ccggcgtgta ggccgcctgg
     1261 gtgccgcgct cgaggccgcg ctggatggcc gcctgcagcc ggctgagcgg cccgtcgccg
     1321 gcgccgttgc ggatgcgcac gtgcttcagg tgggccggca ggatcagctt cgactcgacc
     1381 agcgagaaca gcagcaccgg gatcacgatc agggggatct gcgcgaagat ctggccgcgc
     1441 acgccctcga tcatcagcag cggcatgaag gccaccacag tggtcagcac gccgaaggtg
     1501 accggcacgg cgatctcctg cgtgcccttg atggacgcct gcagcgggtc ctcgcccttc
     1561 tgcaggcgcg tgaaaatgtt ctcgcccgtg acgatggcgt cgtccaccac gatgccgagc
     1621 accaggatga aggcgaacag gctgatcagg ttgatggtca ccccgagcac cggcatcagc
     1681 gcgatgcccg ccatgaaggc caccgggata ccgaccacca cccagaaggc caccgccgga
     1741 cgcaggaaca gcgtcagcag caccaggatc aggatgccgc cctgcaaggc gctcttcagc
     1801 agcgtgttga ggcgcgcctg cacgacgcgc gactggtcgc gccagtaatc gagctggacg
     1861 cccggcggca gccagccggg cggcgaagca atgtagtcac gcaccacccg cgccacctcg
     1921 atggcgctct gctcgccgac ccggtagacg tcgatgaagg ccgccgagcg gccgttgtag
     1981 cggaactcca gcgggtcctc gctgaagccg tcgcgcacca cgccgaggtc cttgacggtg
     2041 agtcgcgtgc cggactcgcc gcgcaccacg atgtcctcga actcgcggcc ggcacgggcc
     2101 tgcccctggg tgcggatgag cacctccccg ccggtcgtgc gcaccgcgcc ggccgccagg
     2161 tccagcgagg tgcggcgcac cgcggccgcc acctgctcca gcgtcagccc gtactggcgc
     2221 agcgtctgct cggagatctc gatggagatc tcgtagggac gcacgccgtc gagctccacc
     2281 tgggtgatct cctcgaggtc caccagctcg tcgcgcacgc gctcggcgac caggcgcagt
     2341 tcgtgctccg gcaggtcgcc cgagatcacc acggtgatga cgtcgcggcg gatctcgggc
     2401 acgtagacca ccggccgttc ggcatcggcg gggaaggtcg agaccgcgtc cacccgcgcc
     2461 ttgacgtcgt tcatcagctc gcgcggatcg aagcggttgt cgacttcgac gaccacggtg
     2521 ccgaggctct ccacggcggt cgagcgcagc tgcttgatgc cgtcgagatc ctggatggcc
     2581 tcctcgatct tgacggtgat gccctcctcg acctcggccg gcgtggcgcc cgggaacggc
     2641 acgcgcacta ccacggtacg cagctcgaag gccgggaaca cttccagcgg aatcctggcg
     2701 ctgagcgccc aggcgccgag cgccagcagg gtcaccatca gcaggttcgc cgcgacgtcg
     2761 ttgcgcgcga accaggtgat catgcctttc actgcgcgcg gccctcggct gcgacgcggt
     2821 cgccgggcgg gccgcggcgg ggcgcgggcg gcacgccgtc gatggtcgcg gccaccagcg
     2881 tgccgttggc ggcgaccgcc agcggcgtaa tgttgaccac ctcgcccggc tccagcccgg
     2941 ccgtgatcac cgcgctctcg ccatccgtcc aggcgaccgt gaccggccgg cgctgcaggc
     3001 ggctctccgc gtcgaccacc agcacctcgg tggcctcgcg caatgcgctg cgcggcagta
     3061 cgaaggcgtt cgccagcacc tggccggcga tctccgcctc gacgaactgg ccgatgcgca
     3121 acggcggctg gccgtccacg ccgcgctcgt agggatcgtc cacctgggcg accacgaaca
     3181 gctggcgggt gcgcgtgtcg atggcaccct cggcacgcac gatgcggccg ctccagcgcc
     3241 actcctggcg cccgatggtg gccttgatga ccgcttccgg acccgtggtg ccgcgctgcg
     3301 cggtctcgcc gcgaaagcgc tccggcaggt ccacgaactc gagctggcgg ttcgacagcg
     3361 gcaggcggat ctccacgtag tcggtggcgt agacgcgcgc cagcagggtg cccggcgaga
     3421 cgaactggcc gacgtcgacg ttcttctcca gcacctggcc gtcgtagggc gcgcggatgc
     3481 gggtgcgttc gaggtcgaac ttcgcacgct ccagctgcga ctgggccgac agcagggtgg
     3541 cgcgtgcggc ggcgatctgc ggcttgcgca ggccgagctc cggcgcctcg cggccgagca
     3601 tgcgccagtc gtccgccacc accttggcct gggcaagctc ctgctcgagc gcggactcgg
     3661 cctgcgccac ctgcgcctcg gcgctggcga tcgcaagctg gtagtcgcgc ggatcgaggg
     3721 tcaccagcac gtcgccctgc tcgaagaagc cgccctcgcg gaaggacggg gagatggcga
     3781 cgatctggcc ggaaacctgc gggatcaggg tgctctcggt gcgcggctcg accgtgccct
     3841 cgctgcggat gaccacgcgg tagtcctccg gcgtgacgcg gatggcgtcg atggacaccg
     3901 gcacctgcgg cggctggaac tgcggtgcct ccggacgctg cgaaacgatg tagcggccgc
     3961 cggcgagcgc cgcgagcagc acggccacgg ggagaatcca cttgagaagc ttgctgatca
     4021 tgcgggcctg tcgcagcggg ggcccgaagt cgcgggccga ccctggttga tatcgaaaca
     4081 atcacgccgc cggatgcagt gcagcggtgg gcgcgtgacg caaggcacgg attttacccg
     4141 cagtcggact gcatattgat attctgggtt ccgggccgga aaagggagtg atcgtgcccc
     4201 atcgcaaagc accctgcctg ggcgcattct gggtcggacg ggtcaactgt gaacgctgca
     4261 gtgccagcgc acccgcgcca ttcagtgtcc tggatgccgc cgagcacgcc cggccgctgg
     4321 cccaggtgga gaactaccgc gcgccggccg gcacggtgat cgtgtcggcg gacgacccgg
     4381 cggaagccgt cttcacgatc cgcgagggct tcgtgaagct gtggcgccgg actgacaagg
     4441 ggaatcaccg catcctgcga ctgctgcgtc ccggcgagat gatcggcctc gaagcgctgg
     4501 ggcagcgccg ctaccagttg tccgcggcgg cgctcagcga cgtcaagctg tgccgcatcc
     4561 cggtggagat cctcgaccag ctgcgacgga acgctcccga gctgctggcg gaactcgacg
     4621 aacgctggct ggtgcaacag acgcgcaccg acgagctgct ggcaaacatc gccaccggcc
     4681 cggcacctga gcgggtgctg aagctgctgc gctttctcga caagctggcc tctcccgacc
     4741 cctgcccgcg aatcagccgg ctggacatgg cctcgatgct cgacatctcc agcgagaccg
     4801 ccgcgcgcgt gattgcggac ctgaaaaagg cggggcggct cgaggaacgg ccagaccggc
     4861 tggaattcga ccccgaccgc ctctagcccg aattggggac attccccaat tcatggaatt
     4921 ggggaatgta cccaattcgg agaaactgtg acgggcgtca cacttttcgc cgcacccggg
     4981 cgttccgact gacggctgtc agtttcccgg cgccgaccgc agcgccccct gcctagcctc
     5041 aggttgcgag tcctggaacg gccggcgcgg ttgccggcgg caggacgcca acaggggcaa
     5101 taccatgaaa cgctcgacac tgacgctgct gacggcgatc tgcgccgcgt tcagcatcca
     5161 ggccgccacg gcccaatccg tcaacggcga cgtcctggaa ttccaaccgg ccgacgagcc
     5221 cagcgtggtc tccgacggcg tatccaccaa caaggtcaag atcctgcgca cgcccgacgg
     5281 cacgctgttc gcggtctatg gggacgagca ggacgtcaac atcaacggta actccatggc
     5341 gtggaacgcc aaggcccacg caccgcaaaa gccgacggac atcgtcatca agtactcgat
     5401 cgacaacggc gccacgtgga gcgcggcgct caacatcgac aacacggccg ccctgtcttc
     5461 cgccaggggc atcctggtcc aggaaggcga gccgatgctg gacccggcca ccgggacgcc
     5521 cgacctcgtc aacgaccccc gcgccgtgga ctatccgggc gactccgaca agccgaacgt
     5581 ctacaacatc ggcaacaaca tcatcgtgac ctggaacggc aagtactgcg cgggcgacga
     5641 acagcgcttc gtcgtgtacc cggagctgtt cggagtcacg gtgcccttca gctgccagta
     5701 cgcctcgcgg ctggtgtgga acagcacgac caaggccttc aatacctacc cggcctgggg
     5761 cgggctgccc tacaagaccg agcagctgac gaccggcatc cgcgacgcca agcaagacgc
     5821 caatcgcggc aacatgtatg cctacgtcct gaactggcag gaagacccgc tgggcctgaa
     5881 gctcggtgaa gccgaggggc cggggagcgg cgcttccggc gcgaacgtca acaacggcac
     5941 ggacgtctgg tacagccaca tcgagctgct caacgcagcg ggcaccggcg tcgacccagc
     6001 caagttcgta aacagctcct ggagcccgcc gacacgcatc acgcgcaata tttttgccac
     6061 tcaacccctc gagggacgcg acatcgagcg gcaccccgcc ggggactata accgcgggcg
     6121 cgtcggtgcg tcacggccga acatcggcca gatcgacgat cacgtgatca tcgcctacga
     6181 ggaaaccaag ggcagcgaag gctgggagga cggcaagtac acccgctacc atgccttccg
     6241 cttcgacacg cccccggaag gcggtgagca cggctgcatc atcagcgatc cctgggagaa
     6301 ttcccggcgg gttcgcttcc tggtgcagtc cgccagcaac gaggtcccgc tgctgttcat
     6361 ctacaagcag ggcgacttca cccagggcgg cgagtccgac atcatgctgc gccgcgcggt
     6421 cggcggcttc atgccggaac acctgcagcc gccggtggac gtcgagaact gccgtgtcag
     6481 catcatggac gacctcgacc cgctgtacag catccgcctc gaggagcacg gcccggcgat
     6541 gaacctgagc ggctcccgcg agatcggcgg cgtgcccggc acgggccgga tgggcgagtc
     6601 catcgacacc ggcgccaaca tgctggagaa cgcgctggcg catcgcggca tgatgcgcgg
     6661 cgacctgatg ctgatcggct tcagctacgt gcccaacctg gcgcagttcc agaacctcga
     6721 cagcgaggcg ccgtacacct tcatggtgcg ccgctcgacg gatgcgggcc tgacctggag
     6781 cgacgccatc aacctcacgc ccgaggtcac ggccgagtcc ggctacaccg tgaaggaacc
     6841 gcgcatcgtg ccgacgcccg gcagcgggcc gcgctgcgcg acggacccga gtgattgcca
     6901 ggacccgaac gtcatctaca tcgcctacgg gatgcaggac aacgtgttcg cataccagga
     6961 ggccggcgac gtcgacatct acatgatggt gaccttcgac tccggcgcca gcttctcggc
     7021 gcccaaggcc atcaccgccg gcgacgcgct gtacggcgta gccgacgact tcgaggactt
     7081 cgagacgcag atcaagctgc ggcccgacgg acgggcgagc tacacggtct ggtccggcaa
     7141 cgacggcgag ttcaagcacg ccatgttccg cagcggcctg gtgcgcggtg atacgcgcga
     7201 gctcctcacc agcggcggcg ccaagtcgcg cctcaccgtg gtgccggatg cccgctccga
     7261 cgccgtgttg accgagacga gctggaagtc cctggcggac tacccggccc cgccggccga
     7321 ctacgacttc ccctacggcg tacttgattt caccgtcacg cgcgtcgcgc tcggcggctc
     7381 gatcgaggtc acgctgagct tcccggagcc gctgcccgag ggcaccagct actggaagtt
     7441 cggcagcact ccggcggcgc ccgaggccca ctggtacgag atccccgcgg tcatcgaggg
     7501 cagcaccatc acctatacgc tgaccgacgg cctgacgggt gacgacgacc tggcggtgaa
     7561 cggcgtgatc gtggacccgg cggcaccggc cgtctacaac cccaccgtca ctccgccgcc
     7621 cccggcgccg acgccgagca gcagctccgg cggctgcacc atcgggaacg gcgacaaggt
     7681 cgacccgacc ctgttgctgc tggcatctct cagcctgctg tacctcgtac ggcggcgcag
     7741 gatgcctgtc tgctgagctt ccccctgtaa gttcagccaa ggcaggaggg ggcgcccgca
     7801 agggcgccct cttcttttcg cggcgagctc ccgccgccgg cgcggcagtt caggcgccaa
     7861 acagccagaa gaagccggcg ttgtagaaca tgtgcagcgc gatggcggcg ccgacgccgt
     7921 ggcgctcgcg gaaaccaccg aacaccagcc ccggcaggat cacggccgcc gcccaggccg
     7981 gcgaagtccg cagcaggtgc gccgtggcga agcccagcgc cgcggcgaga ttggcggcgc
     8041 tcaccgggcc cgcgatgcgt cgcccccagc cgcggttcag cagcgcgccc tgcaaccagc
     8101 cccggaacac cagctcctcg aggaccggat acagcaccgc cagcaccagg aagcgccccg
     8161 gctggacggc cggccagccg ggctctgcga gtggcagcca cagggccagc agcgcccacg
     8221 cggctggacc ggcggcaagg gcgaagaaga accaggggtc ccgccatggg cgcgttgcgg
     8281 cctgaagcgt gatcatccag caagaatgcc acgcgcgacg ggatggctgc attcgtacaa
     8341 gccagtgcga gggcggtggc atcgacccgt cgcagttggc ttgcaccgca cagtcggcat
     8401 caagcaacct gcaaccatga atctcgagct cagcgaagcc gccatccgtc tcggcgcctt
     8461 tgccgcacta ctcggcctgc tgctgttcgc ccagtggcgc tggccggcgc gcggcgacgg
     8521 ccggccggca cgacgccagc tggtcaatct cggcatggtg gccatcggca ccggcctgct
     8581 ccgcgtcggc tttcccgtgc tcgcggtggc ctgggccgtg caggtccacg gcggcggcct
     8641 gttcggcctg ctggactggc ccgcctggct ggccatcccg gcggcagtat tgctgctcga
     8701 cgtcgccatc tactggcagc accggctgat gcatgcctgg ccgctgctgt ggcgactgca
     8761 ccgcgtgcac cactgcgaca cggccttcga cgtcactacg ggcgtacgct tccacccttt
     8821 cgagctcgtg ctgtcgatgg gcatcaagct ggcgctgatc tcgctgctcg ggccccaccc
     8881 cgcggcggtg ctcgccttcg aggtgctgct cagcctgggc gcgctgttca cccacaccga
     8941 catcgccctg ccgcgggcgc tggaccgcag gctcaggctg ttgttcgtga cgccgtccat
     9001 gcatcgcatc catcactcca cccgccggat cgagacggac agcaattacg gctttcacct
     9061 gtccgtctgg gaccggctgt tcggcagcta caccagggag ccggtggagg acgagcgacg
     9121 catgcccatc gggctggagg agtggcggga accgcgcgac cagggactgg tctcactgct
     9181 cctgaacccc ttccgcgcgc ccggcccaag tcatgggggg aacgggccgg atacgagcga
     9241 gcgccatgcg tgacaccctg cccctgctgg tcgacaccga cttcccgcgc atccggcgtg
     9301 accgcctgga gacgctgcag ctcaatctcg gctacctgtg caatatcgcc tgcgtgcact
     9361 gccacgtggc ggccgggcca acgcgcaagg aactgatgga ccgcgccacc atggagctgg
     9421 cgctggccgc ggcggaacgc cacggcgtcg gcaccctgga cctgaccggc ggctcgccgg
     9481 agatgaaccc ggagttccgc tggttggtgg gcgaggcgcg ggcgcgcggc ctgcacgtca
     9541 tggaccggct gaacccgacc atcatgcgcg agcccggcta cgagtgggtg ggcgagttcc
     9601 tcgcgtcgca ccaggtggag gtcatcgcct cgctaccctg ctattcgaga gaaaacgtgg
     9661 acgcgcagcg cggcaacggc gtgttcgaag cctccatcag cgcgctgcag gaactgaatg
     9721 cccgcggcta cggccggccg gattccgggc tggtgctgaa cctggtctac aacccgctcg
     9781 ggccgtcgct gccgccgccg cagcaggcgc tggaggccga ctacaagcgg ctgctcggcg
     9841 aggagttcga catcgttttc aacgccctgt tcaccatcac caacatgccc atcaagcgct
     9901 tcggcgccgt gctgctggcc aagggccagt acgacggtta catggagacg ctgcgcaatg
     9961 cccaccgcgc ggccaatctc aagaacgtca tgtgccgcag cctggtcagc gtggattacc
    10021 ggggcttcct gtacgactgc gacttcaacc agatgctcgg catgccgctg ggcggggcgg
    10081 agtccgggcc gcacctgcgc gacctgctgt ccgccgacga gtcgccgcgc ggcatcgccg
    10141 tcggcggcca ctgctacggc tgcaccgccg gccagggctc cagctgcggc ggcgcgctcg
    10201 cctagcggcg gctgtcgcgg cgcgcctggc tgcgtttccc cgcagccgtc ttctggccct
    10261 tgcgcgcacc ggcgcgcgtg tcccgggcca gcttgccgag cgccgacacc cgcgccgatt
    10321 ccgccgcata accggtggcg ccggctgcct tgcgactccg tgccttgccc ttgccgagcc
    10381 tggcagcgcg gcgcgcgccg gccttcttct tgtcggcgat gcgtgtggcc agcgccgcct
    10441 ttgccgcggc gcgcttcgct tcgcgttgct ccgccgcagc gtgtttcgca gccttcgcct
    10501 cgaagcgccc aagcgcctcg tcgaacagtt tcgccttgtc cgcggtgcgc acattggtct
    10561 gcgcgcggtc gcccttcttg gtacccgtgc gcgcgcgatt ggccagacgc tggcgtttca
    10621 ggaggtcacg gtacttgtcg cgcagcttgc gcgcgcgctg gatcttgccg cgcaactgaa
    10681 ccggggtgtg cttgccgatc tcgtcggcca ggctggcggt gaacaactgg tactcgctgt
    10741 ccgtgcacaa ggggcgggca tgattgcggt tgaaggccat gggcgatgtt ccttcgcgtc
    10801 agaagtagta gccgacgttg atgttgagcc gggagcgcca cttgccggct gaagcgccat
    10861 ccagaccgat cccgtcgccc ccagcaaacc acatgttgcg ccccgcgatc cagtcgaaat
    10921 aggcgaacac gccgccggcg gataccgagc agccggtgac gttctggatg gactcggcgg
    10981 aattgtcgac gtccggatcg atgtaagtga agttgttgta gcagttgagt ccggtgatcg
    11041 ggccccattc gacctcgaag gatcgcgcga tgttggccga cagcacgttg cctctcgccg
    11101 ccatcaggaa gggaaagtcg aaggccccca gctgtacgaa gtctgccgac accccggcct
    11161 ggttctcggg cttgaactcg tagcgaatac cctgcagctg caagttccac ggcccgtaat
    11221 tgccgttgac gtgcgcgccg agcgcccagc gatccccatc gcgctccgtg gcgcggttgt
    11281 agatctggcc ggcctccagg ctcaagccga tctcagtgct gcgctcattg ccgtgctgca
    11341 gcaaccgcgc cgcgcgaaag ttgagctggt tggtttcgct gttctgctgg tcgccgcccg
    11401 tgacgaggtc gaaggagtag cgtccgtagc ggccgtcgtc gacatactcg gggttcttga
    11461 aaaaggcgta gtggaaggtc cagtcgccac tcttctgcac gaacttgacg cccgtgtcgt
    11521 agtcgtcctc gaaacccatg taatacgtgc cgccgaacca gaagctgtgc gaggcgtagg
    11581 gcaggatgcc gaacggtacc tgcgtgatgc cgagttcgac atggcggtcc tcatcgaagc
    11641 ggtaaccgac ccaggcgtgg tgaatggcct ggaaatcgtt gtagcggcgc cattctgcgt
    11701 cgaggatcac gtcgccgacc gtgccgcgcg cgttgatgcg gaacagctcg agctcgaagt
    11761 agccgaggcg gtccttgttc tggtcgtcgt agtcacgcca gccgtaattc agccgcactg
    11821 cgccgccgaa attgatgccc tcgtcctctg ccttcgtggt taccggcatg gccagcagaa
    11881 gcgcggcaag cgccgtgata ccagtcgtga cgtgtccctt gttcatttca gatcctttcg
    11941 aactcttcgc ccgtgcaaat ttgtggcggc gccgcaagcg ttgttaagat ggccacctcg
    12001 acccaccagt gtaggcactc tggagcggag cgcaacccgc tgttttgctt aggttttaac
    12061 tgccctgggc aacgggcggg tgacgcctgt tgggcgcccg ggcgcagcgg cgacgaggcg
    12121 cgatgaacgt cacccacgcc cgcattccga cgctgatgct ggtcctgttc gggctggtca
    12181 acctgccgct gtccatgctg atgtcgccga ccgcggcgat cctgcccaac ttctatctcg
    12241 aatacagcgc ggtcaccctc gccggtctgg ccacggccac cttcgtcgcg agactgttcg
    12301 acgcggtgac cgatccgctg atcggctacc tgtccgaccg gtccgggtcg cgcaagccct
    12361 ggatgattct cggcacaggc gtggtggccg ccggcacctg gttcctgttc aacccggcga
    12421 gcgacgcggg catcggccac ctgctggcct ggtacctgtt cgtgacgctc ggctggacac
    12481 tggtcgagat tccgcacacc gcgatggcgg ccgagctgtc cactggctat cacgagcgct
    12541 cacgcatcgc gctgtggcgc cagctgctcg gcttcgccgg cggaatcctg ttcatggctt
    12601 ccccgatgct gctcatcggc agcgccacgg agttcagccc ggccgtgatg cgcaagctgg
    12661 cgctgttcat catgctggcg ctgccgttgt tcgtcgccct gatgtgcctg aaagtgccgg
    12721 aaccggcccg ccgggtgcgt gcgcgaccgg tacagcccgc cgacctgctg cgcgcgcttc
    12781 gcgatacccg cccgctgcag tacttcctgc tcacccaggt gctgttcggg ctggcgacgg
    12841 gagcggtggc cagcctgttc gtgatctaca cgcgctggca tctcgggctg ccggacaagg
    12901 tctcgcacat cgccgtgccg atgaccctcg ccatggccct cggcatgccg ttgtggctgc
    12961 aggtcatgaa acacgtcgac aagcatcgcg cgtgggcgat ggcggcggtc ggcatgatcg
    13021 tcacactgtt gctggtgcca tggatcgagc ccggacccgg cgcgctgaag ccgatgacgg
    13081 gcgtcatggc cagcttcggc tttttcctcg gcctgtcttc catcgcgctg ccctcgctgc
    13141 tcgcagatat cgtcgactac gacatctggc gcaaccgccg ggaccgcgca gcgatcttct
    13201 tttcgttcca ggcgctggtc accaagctca accagggcat cggcggcgcc attgcgctgg
    13261 ccataccggc ctcgttcggc ttttccaccg aggcgccgct gagccagcag gcggtactgg
    13321 gcctgaaaat cgccttcgtg gcgtggccgt gcctgttgtt gttgccgatg gtggtgctgg
    13381 cgtggcgcta cccgctcggg cggcgcgcgc acaccacgct ggcgcggcgc atcgatcgcc
    13441 ggggtgaacg gaggatattg cgggggtctt gacgtttcag ggcgaaggca gcgcggtgat
    13501 ctcggtcact gcgtgcgctt cgccctcgtg gtcgaccggg tcagcggggt gctcccggtc
    13561 aagcgcgatc cgcggccttc aggcgcgacg cgcttgcgcc tggcggcgcc tccgacggga
    13621 ccggcgacca agccgggcct ccatgtcgct gggggacgtt cttgcccttg cagcgctctt
    13681 gcgggtttcc atggcagtct ccgtggctct gaataaagct taaccggcca gaataatatc
    13741 gacgggggca ttaccggaac ttgatctgga tcaagttcgt agccgattgt tttaagtact
    13801 tctgctgatc gtgaagcccg tcacacggcg cagttcgccg gcgtatttgc gacggattca
    13861 gcggccgccg gcggggctca ttcagcgtcc cggccaggga tgcaggtcgg cggccagcac
    13921 ggccagcgcg gaaccgacgg tgtgatcggt caataccggc gtgagcagga tcagcaccgc
    13981 ccaggtggtg gtgaagacca cggcgaactt gtcgccacgg ctccatggcc gccgccaggg
    14041 cgggccgtcg tcgggcactt cgtcggccgg ctgcgcgggt cgtttcgccc cgttgcgggt
    14101 cgccacggcc ttcaccacgt gcgccgcctc gtcggccgtc atcagggcgc cgcggatggc
    14161 ccgctgccag gctatcgtgc gcgctgcagt ctgggcgccg gcgcgggccg cggcgccgag
    14221 gcccgcgagg ccgaacagca cctcgatgta gaggcccagc cacagcgcga ccagcgtgag
    14281 gccgatgtcg agcttcaggt gccagagcac ccggccgagc ctgttgtggc ggatgccctg
    14341 ccgccccatc gccacccact cgagcacgcc gtgcgcggcc acgaccgccg ccagccagaa
    14401 caacgagatg gtcatgctga gcacgagccc gaggcccacg tacagcacga tgaacagcca
    14461 gctgtcgtcg tgctccgcaa cccagtgcaa cacgcgccgg cgcagcgtca tttcggcggc
    14521 aggtgtcgat gagatggcgc gtcctccccc gtggcagggc cgagtctata aaaaaaccgg
    14581 gggaaagcag aaaaaatcgg ggccaggtac gcatttcgaa cgaaatgcgt acctggcccc
    14641 gattttttcc cggttttcag ctgcgcaggt agttcagcgc caccctggcg ataccggccg
    14701 gcttgaagct gagcatgtgg cgctgccaga acagctcctg cttgtcgcat accgtgttct
    14761 ggctgtagct ggcgatctcg ccgcgcagct gctcggcggc ggcgggatcc atctcgccgt
    14821 ccatgccggc attcaccctc gcacgcagcc gccgggcctg ggcgatgccg tcggcgaccg
    14881 ccctggcctg cttggcagaa atccgcccgt agcgcagctt gagttcgttc agacgctcgg
    14941 ccgcatcata ggtcgcgtcc accagctggg cgcgcgtcat ccactcggtc tcgtagttga
    15001 ggatgtgctc ccagctcggc tgctcgagca agcggcgatg ctcctccagg gtatgggcca
    15061 gcttgcggta gccgaagcgc tccgggtgct cgaagatctg gctgccggga tcgaggaacg
    15121 gccccatcgg ggaaatgaag cactgcagcc gcttgtcgcc ccagcggaac agctgctcgc
    15181 agtagtcgac cgtctccatg acggactggt acgtctgctg cggcaggccg atcatgtaga
    15241 acacgtcgat gcggtggcaa ttcagcttga gcgcctcccg cagcatggtc tccatggcct
    15301 cgttctcgta gcccgcctgg ccctccatgg catggcggac agtcttgtcg tggctctccg
    15361 ggctgacctc gaagctccag ttgggcagct tcccgtcgat gtagcgcagg aagtcgatcg
    15421 gcggcacgtc gaagaactcg aagcagatct cgttgccgac gcccgccgcg gcgatctcgt
    15481 caatgcaccg cgacgcgtac ttgcgcccgc cctggagcag gtcgccgggg atgacgatcg
    15541 gcccgcggga gaagcgactg atggcggcga gattggctgc caggttggcc gggctgcgga
    15601 acaccggctt gcggcggccg gtcatcatct ggcaggtgga attcgagctg ccgcaggtca
    15661 cgcactcgaa ggcacagccc ttcagcggca acaccatggt ggtggggttt ttcagccagc
    15721 cgttgaaggg cagcacgctg gcgaaatcgc ggtagcggat cgccatctcg atcaacagct
    15781 cgggatggac gtcgatgtag tcgagcgtcg gcggcacgaa acccagcgga ttgacccgga
    15841 cctcgtcccc gtcccgccag gtcaggtttg gaatcccgtc cagcgggccg ccgtcacgca
    15901 gcgccagcag cagctggtgg agcggcggct cggtgctgtc gccgcgcagc acgaagtcga
    15961 tctccggata ctgcagcagc tcctcgtgaa agtaggttgc cgagaggccg ccgaacagca
    16021 ccggcacccc gggatgcact tgcttggcga gccgcgcgat ctcgatcgcg ccgtgacagt
    16081 gaggcatcca gtgcaggtca atgccgatgg ccttcggccg aaccccggcg aggaacttcc
    16141 gcacgtcgaa atcggggtcg gtgagcatcc gcagcccgag attgacgatg cgcacctgca
    16201 ggccgcgctg cttgagatag ccggcgatgg tcaggaagcc gatcgggtag atctcgaaca
    16261 ccaccgacga cggcaccatg tcgctgaccg ggccgtacag gatcgacttc tcgcggaagt
    16321 cgtacacgct cggggggtgc agcagcagca ggtcgacggt cttggcagcc atgccggaac
    16381 agcctcgtga ctccgggagc ccagtctgtg gcaaggcgcc ggcccgcgcc ataccggatt
    16441 accccacccg acaagggtgg acaggcccta gtccctgaag ggcctcacgc gcaccccgtc
    16501 gtccaccgca tcatccggat gcacgatgac cgcgtcgccc tcgcccagcc cctccagcac
    16561 ctccgacagc aacccgctgc ccgcgccgag ccgcaccggc cgcagcctcg cgcgatcgcc
    16621 ctcgaccacg aacaccgccc agccgtcgtc gcgacggaac agcgcgctgg caggcaccgt
    16681 cagggcgtcg ggccgttccc agacgatgaa cctggcctcc acgcggtagc cgtcgccgag
    16741 ccgctgccag gcctccggcg gcgagctgat gtcggcgatg gccagcacgc gctgctcctc
    16801 gacgccgagc gcgctgattt tcgtgaagcc ggtcggctcg acggtgcgca ccacgccctc
    16861 cagcgtctcg ttgccgcccc agcgctcgaa cagcacgcgg ccgcccggct cgatgcgcac
    16921 cgcatcgcgg gacagcacct ccacctcgac ctcgagacgc cgcggatcgc ccacctcgag
    16981 cagcgcttgc ccggccgaca ctacgccggc gctttcctgc aggactttca gcacgcgccc
    17041 ggccaccggg gagcgcaccg gcacggtctc ggccggcgcc tcggcctcgc ttgcgccgga
    17101 atggcgcagc gccgccagcg ccgcctccag ctcgaagcgc gccacctcga ccgcggaggc
    17161 ggcggacgcc tgcgccgccg ccatggcgtt catgcgactg cgcgcctggt cgaattcgga
    17221 ccgggaaatc agttgccgtg ccagcatttc ctcgcgccgg cggaactctt cggcggccag
    17281 ctcggcctcc gccgccgcct ggcgcagcac ggcccgggag cgctccaggc cggcgcggcc
    17341 cgcggccacc cgtgcctcgg cctcggcccg ggcgcgcgca tccagcacgc ccggccgcag
    17401 cgggtcgagc accgcgagcg cctggccgag agcgaccgcg tcgcccacct ccagctcgac
    17461 gcgccgcagg tatccggcca ccggcgcgta cagcacgaaa cgatccacca cccgggtgcg
    17521 gccttcttcc tcgaccgtga cctgcaacgg ccggcgcgcc acctcgccgg tctcgatcag
    17581 gaccgggcgc ggccagaagc cccacgcgat cgccagcgcg accaccgcgg tgacgacgaa
    17641 tattcccttg aagcgtcctg cgggcacgat ctactccctg gtcttcagca cctcgacgag
    17701 gtcgagctgc gcgagcttgc gccagatcag catgccggtc acggcaaaag ataccagcac
    17761 gatcgaggcg gcgagcgcat aagtggccgg ctcgacgatc agcgcgatgc g
//
LOCUS       DAOMPG010000015        17797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1399_length_17797_cov_4.159790, whole genome shotgun sequence.
ACCESSION   DAOMPG010000015 DAOMPG010000000
VERSION     DAOMPG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17797
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_17797_cov_4.159790"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLS34_01545"
     CDS             complement(<1..307)
                     /locus_tag="PLS34_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="controls initiation of DNA replication by
                     inhibiting re-initiation of replication, promotes
                     hydrolysis of DnaA-bound ATP; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HRP86187.1"
                     /translation="MRQLPLAVRLRDFAVFDSFEPGPNGAVVAALAEPAATAPALWVW
                     GPDGSGKSHLLQAACAAHDSAAYLPLRELLTAGPEVLQGWEDRGMVCIDDVDELAGRR
                     "
     gene            complement(307..855)
                     /locus_tag="PLS34_01550"
     CDS             complement(307..855)
                     /locus_tag="PLS34_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRP86188.1"
                     /translation="MRARDIPNLLCVLRIALTVPIGWSIVEGRYPAALVMFFIAGLTD
                     ALDGFLAKRFHWQSRLGGFLDPAADKLLLVTAFVTLWAAGYVPGWLLAAVVLRDLVIV
                     AGAGVYQWRVGDFTAEPTIISKCNSFLQLLYILLSLAALVFALPPRGLIDGLGWAVLA
                     TTIVSGCDYVIRWSGRARAANA"
     gene            complement(852..1778)
                     /locus_tag="PLS34_01555"
     CDS             complement(852..1778)
                     /locus_tag="PLS34_01555"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HRP86189.1"
                     /translation="MEGLYQAEAQLTDGRDEAFRAALGQVLVRITGRRDAAGRPEVAK
                     LLDNAAAYAQQFRQPAPDRLWVAFDGAALERELGRLEQPVWGPERPSTLLWIAVDAGG
                     GRRFVVASGAESSEEAAIRAEVLEAAAERGIPVVFPLMDAEDRARASFAEVWGGFEDS
                     ILAASERYGVDAVLVGRLSAGDLGHGRWTLHGPDGAERWSGGIPDSVGRLADRFAARF
                     AVVASGTGRILQVAVSGVATVEDYGRVSTFLGRLTAIESVGVESVVGDRVVFRVAVRG
                     EASALDEAVRLGGLLRPDRDNLAHDLNYRVAR"
     gene            1933..2964
                     /gene="purM"
                     /locus_tag="PLS34_01560"
     CDS             1933..2964
                     /gene="purM"
                     /locus_tag="PLS34_01560"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRP86190.1"
                     /translation="MKDTSSLTYKDAGVDIDAGNALVERIKPFVAATRRPGVLSGIGG
                     FGGLFEIPSGYRRPVLVAGTDGVGTKLKLAIDTGRHGGVGIDLVAMCANDVIVQGAEP
                     LFFLDYYATGRLDVDSAAAVIEGIAEGCRQAGAALLGGETAELPGFYAGKDYDLAGFC
                     VGIVEYERIIDGSRVAPGDAIVGIASSGLHSNGYSLARKVLERSGAPPDMLLDGVPLI
                     ERLLAPTRIYVKPLLALLAAVEVHAMAHITGGGLPENLPRVLPEGAAARIELASWRRP
                     AVFDWLQAEGGIADAELYRTFNCGLGMAVVLPAADADRAVALLNEHGEQAWLAGRIEA
                     GERRVVLAG"
     gene            2961..3605
                     /gene="purN"
                     /locus_tag="PLS34_01565"
     CDS             2961..3605
                     /gene="purN"
                     /locus_tag="PLS34_01565"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRP86191.1"
                     /translation="MSRRPLPVAVLVSGFGSNLQALLDAADAGRLNVEFRLVASDRPE
                     APGLKRARRAGLATAALPPDDYADRTAWNDAMATALRDSGAELVVLAGFMRVLGAELV
                     RAWQGRMLNVHPSLLPLHRGLHTHRRALQAGDRMHGTSIHFVTEELDGGPVILQARVP
                     VLPGDTEERLTARVQAREHEVYPRVVQWIADGWLEWRDGGPWLFGRRLAEPVVV"
     gene            complement(4003..4569)
                     /gene="dcd"
                     /locus_tag="PLS34_01570"
     CDS             complement(4003..4569)
                     /gene="dcd"
                     /locus_tag="PLS34_01570"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRP86192.1"
                     /translation="MSIKSDRWIRRMAEQHGMIEPFEPGQVRAVDGRKVVSYGTSSYG
                     YDVRCAPDFKIFTNINSAVVDPKAFDESSFVDVSGAVCIIPPNSFALARTVEYFRIPR
                     QVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFL
                     ESDEECEVSYADRGGKYQGQRGVTLPKT"
     gene            complement(4570..5664)
                     /gene="apbC"
                     /locus_tag="PLS34_01575"
     CDS             complement(4570..5664)
                     /gene="apbC"
                     /locus_tag="PLS34_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRP86193.1"
                     /translation="MTEATIDTARALLAQRIDPVIEQDLVSAGAIKDLALKGGVLSVV
                     LRLGFPADRYERELQAEFTGLLGSLPGVSSVKVEIQWDVETRSVQPGLSPLAGIRNII
                     AVASGKGGVGKSTTAVNLALALASEGAKVGILDADIYGPSQPRMLGLSGRRPTSTDGK
                     RIHPLEAFGIACMSIGFLIDEDQPMIWRGPMVTQALQQLLGETHWGDIDYLIVDMPPG
                     TGDIQLTLSQRVPVSGAVIVTTPQDIALLDARKGLRMFEKVKVPVLGIVENMSLHVCS
                     NCGHQEPIFGSGGGERMAEQYGVPLLGNLPLDIHIREEADAGQPSVVSDPDGPAARAY
                     LDIARRMSARLAAGGELPGAKFPTISFEDD"
     gene            5836..8232
                     /locus_tag="PLS34_01580"
     CDS             5836..8232
                     /locus_tag="PLS34_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP86194.1"
                     /translation="MSLIGGFVADRLVAGIRGPADLESAAGKKAIARLRKVGGPAVTK
                     LVAELESADKERAAALARVLGTLISVRTLPEAADALRHTNARGLKGLVWALSNSKDYN
                     PNKLLDYLSEEGMPRAAVLEILEAQKDRLEFRPVLNRAYQVESTEKAALFKLLGEMAD
                     DEMLPELVSRLSGKDVAAKVHIMDILSRFDRPEVRHALTEELGSDNKLVRQTALAALG
                     RLGGELDIARLCKLLMDPDISVQNRAVDVIIKQRHPDTLRHLVEVLKSESDFARRAAV
                     EVLNEIGDQESIKDLLSALSDDDWWVRARATDALARIGGPRVIDAVLGLINDEDETVR
                     RSAVEILNTTKDERAVQHLIKATTDPDWWVRERSADALAEIGDVRAVPALIKMLGGEP
                     RSVPAAVRALAKLAGGDAVAHLKPLLEHKDPQVRVEVMAALAETADPSAAESIIQRIA
                     KEGSSKETTVSEAAASAIQRLSERMGTATTSGTLASGTGGAAAAASLADTKDLRELLK
                     AAAQQSDALDITKLEPGDTIEGRYKYIRKIGKGAFGTVLLVEDMVVDERLVLKFLNPG
                     VAADEEMMKRFVHELRYSRKITHPNVIRIYDFIALGGLYAISMEYFASHPLSDELKDE
                     KPLPLKRTLQYGMDIATGMQVAHQAGVIHRDLKPANVLINDDSLVKVVDFGVAAARAS
                     GDTQLTRTGFVIGSPKYMAPEQILGKKVDEKADVYSLGVMLYEMLTGFAPYTRGDHMS
                     VMYQHVQGKAKRVRELNPNVPQELSDLVARCMAVDKSKRYPSMDELRHALAPFVGTET
                     "
     gene            8236..9327
                     /locus_tag="PLS34_01585"
     CDS             8236..9327
                     /locus_tag="PLS34_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRP86195.1"
                     /translation="MARIDAFLQLAMAQGCSDIHLAVGVPPMLRLRGDLMPIKFRDLS
                     PSELESYVNEILTPAQKKTFSGGHDLDFSYVSGDGGRFRVNLFRKDTGPGAVFRFIPA
                     EVPTLESLGLPPIIRQLCDYHQGMVLVTGATGTGKSTTLAAMIDFLNSTRKMNIISLE
                     DPIEFVHKCKNSQVVQRELGTHLTSFSAGVRAALREDPDVILVGEMRDAATISMAMTA
                     AETGHLVLGTLHTTGAVKTIDRIIDALPAQQREMTKSFLSQSLVAVVTQVLVKTADGQ
                     RRKAIHEILVQNKAIAKLIMSDQTHLIPSQLQTGKEAGMQLMDQALLEAIQRREIDPD
                     DAYRFTTDKKQFARFVTDTGILPKLDLAE"
     gene            9324..10466
                     /locus_tag="PLS34_01590"
     CDS             9324..10466
                     /locus_tag="PLS34_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRP86196.1"
                     /translation="MTGTNPTLTQRNSQLVDRLLRAMVERGGSDLHLIAGNPPRFRLH
                     GDLQVLEQTFLDQQELAQNLQAIATPLAVATFEKEEQADFAYMIQGVSRFRVNVFRHL
                     GGMGAVMRAIPSEAVSLESLKLPEVVRSLCQHRQGMILVTGKTGSGKSTTLAAMINEI
                     NLRRKGHIITIEDPIEFVHQRQGCLISQREVGNHTPTFSEALISALREDPDVVLVGEL
                     RDLETMSTAITAAEMGILVMGTLHTNGAAATVDRIVHAFPPEKQPHVRTMLSTSLRGV
                     ISQQLCRRKTGDGRIAALEILINTPAVSNLLRQGKLDQLETAMQSGANVGMRTMDSAL
                     QELLDRRFITPREAYDKALSKDRFRKLVEQSEPPPLRGGAKPPDMA"
     gene            10504..12537
                     /gene="metG"
                     /locus_tag="PLS34_01595"
     CDS             10504..12537
                     /gene="metG"
                     /locus_tag="PLS34_01595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRP86197.1"
                     /translation="MSESREILVTSALPYANGPLHLGHLVEYIQTDIWVRFQRMRGHH
                     CLYVCASDAHGTPIMLKARQDGITPEQLIREVHAGQLEDFAGFHIAFDNYHTTHSPEN
                     RALVEEIFHALEAAGHIETRSIRQSYDEQEGMFLPDRFVRGTCPRCGAADQYGDSCEV
                     CGATYRPSELLEPVSVLSGTRPVERESEHLFFRLGDFEDMLKRWMNEGRLHESMRNKL
                     EEWFEAGLQDWDISRDAPYFGFEIPGRPGKYFYVWLDAPIGYIASFRQLCDRRPDLDF
                     DAWWRPGGDKELYHFIGKDIVYFHSLFWPAVLHGSGFRTPTAVFAHGFLTVNGEKMSK
                     SRGTFIRARTWLEHLDPEALRYYYATKLGPGTEDIDLNLQDFVQRVNSDLVGKLVNIA
                     SRCAGFIHRVAEGRLAATLPEPALHREMAAAGPAIAECFEQRRYGQAVRQVMALADRA
                     NQYIDREKPWQMAREPGREAEVQSVCTQGLNAFRSLVTWLKPVLPRLAEAAEQFLQCP
                     PLAWDDAGTPLLDHRIGPFEPLMQRVDPAAVQGLLEASRDSLEKKPAPGAARAAPKQA
                     EELPAEIGIEEFMKVDLRVARILEADYVDGADKLLKLVLDVGNGRRQVFSGIRSAYRA
                     EDLPGRLTVVVANLKPRQMRFGLSEGMVLAAAGGDGIFLVAPDSGAEPGMRVT"
     gene            12565..13146
                     /gene="rsxA"
                     /locus_tag="PLS34_01600"
     CDS             12565..13146
                     /gene="rsxA"
                     /locus_tag="PLS34_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HRP86198.1"
                     /translation="MKELGLILVATVLVNNFVLVKFLGLCPFMGVSRKLETATGMALA
                     TTFVLTLSSVCSYLVQEWLLAPLGLEYLRTIAFILVIAGVVQFTEMVVHKTSPLLYQV
                     LGIYLPLITTNCAVLGVALLNVQESHGFLESFVYGFGAALGFSLVLVLFAGVRERVAA
                     ADVPVAFRGASIALVTAGLMSLAFMGFAGLVRG"
     gene            13160..13717
                     /gene="rsxB"
                     /locus_tag="PLS34_01605"
     CDS             13160..13717
                     /gene="rsxB"
                     /locus_tag="PLS34_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018875020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HRP86199.1"
                     /translation="MISAVVVIAGLATLFGLALGFAAVRFRTEGDPVTDRIDALLPQT
                     QCGQCSFAGCRPYAEAIAAGEADINRCPPGGEATIRALADLLERDPKPLDPESGEIRP
                     RTVAVIDEPLCIGCTLCIQACPVDAILGAGKQMHTVITSECTGCELCVAPCPVDCIAM
                     VEEPVTLKTWKWPRPDDPAQPAERR"
     gene            13714..15270
                     /gene="rsxC"
                     /locus_tag="PLS34_01610"
     CDS             13714..15270
                     /gene="rsxC"
                     /locus_tag="PLS34_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRP86200.1"
                     /translation="MSKPRLWHFHGGLKLDPRTARPASVPLGQVPVPARLVLPLSQHV
                     GQPAEPVVTIGERVLKGQPVARPSGYIAAKVHASSSGTVVEIGDHPVPHPSGLSAPCI
                     VIETDGLDEAWEGYEPLAEFAQLNSNELRARVRAAGVVGLGGAAFPTAVKLGSGTGLK
                     LLVLNASECEPYICCDELLIRERPEEVLTGARVLMRALGIDRCCIAIEEDKPEAKRVL
                     LDAIQAAGETRIEVVAVPAVYPEGGERQLIKVLTGEEVPARGVPPDIGYLCQNVGTAA
                     AVARAVVRGEPLISRIVTVTGAGVARPQNVEVRLGTPVADLVRACGGYTDDAAYLLMG
                     GAMMGFPLADDTLPVIKGMNCVVVASREEIRPRAPAMPCIRCGECAEVCPAQLLPQQL
                     YWHARAGELHRLDEYHLFDCIECGCCDVACPSQLPLTQYFRYAKTELWSRGRDRARSD
                     LARERFEARRQRLDAEQEARKRRLEEKTRAADQARGDEQARKAMIDEALERVQARREQ
                     DQDGRAEREE"
     gene            15267..16310
                     /locus_tag="PLS34_01615"
     CDS             15267..16310
                     /locus_tag="PLS34_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRP86201.1"
                     /translation="MKFERTAPPHVRPATSVTRVMGEVLLALVPATVAWVWFFGPGLL
                     YNMAVAAAVALACEAGVLALRRRPVGPQLLDLSALVTAMLLAFCLPPLTPWWITATGA
                     AFAIVFAKQLYGGLGYNPFNPAMAGYVVLLISFPEQMTRWLPPLGDSLGLAAASLDPW
                     QTLRFTLTGLLPDGLSWDAVSGATPLDDLRTQLSMARTVEEVRAGALYGSLGARGWEW
                     TANWIALGGLYLLWRKIIRWQIPVAVILGVCVPAAFFWLIDPGAHPAPTFHLFSGATL
                     LCAFFIATDPVSAATSRSGRLVYGFGIGVLIFVVRSWGGYPDGVAFAVLLMNMAVPAI
                     DHFTRPRTYGHAG"
     gene            16300..16977
                     /gene="rsxG"
                     /locus_tag="PLS34_01620"
     CDS             16300..16977
                     /gene="rsxG"
                     /locus_tag="PLS34_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HRP86202.1"
                     /translation="MRAEIVRSMLRAAGILAGFAVFGALLVAVTWDSTEERIAANERA
                     FLLRSLADVLPDDGYDNAVHEDVVMVTDAALLGTSAPLSVYRARLRNRPVALVLTPVA
                     PGGYSGPIKLLVGVLADGTVSGVRVVAHRETPGLGDKIELERDDWILDFDGRRIGQPP
                     RERWAVRRDDGVFDQFTGATITARAVVTAVRDALLYFEAHRDELFAAPAGATLAPPAQ
                     RDDEDEQ"
     gene            16974..17660
                     /locus_tag="PLS34_01625"
     CDS             16974..17660
                     /locus_tag="PLS34_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRP86203.1"
                     /translation="MSVTPAEVVRTGLWTNNAGLVQLLGLCPLLAVSTSLVNGLGLGL
                     ATTLVLVASNLTVSMIREFVRPEIRIPVFVLVIASFVTAVELTMNAWFHELYRVLGIF
                     IPLIVTNCAIIGRAEAFASRQPPGLAALDGLSMGVGFTGVLVTLGAARELIGQGTLFA
                     GAHMMFGEGARAIELQFADGGFLLALLPPGAFLGLGLMVAAKNFLDARLERRPAPAPA
                     TPAAAAGETA"
     gene            17675..>17797
                     /locus_tag="PLS34_01630"
     CDS             17675..>17797
                     /locus_tag="PLS34_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRP86204.1"
                     /translation="MNNTRRRELYERLRAANPAPTTELNYRSPFELLVAVVLSAQ"
BASE COUNT         2757 a         6225 c         6110 g         2705 t
ORIGIN      
        1 cacgccgtcc ggccagctcg tcgacgtcgt cgatgcacac cattccacgg tcttcccagc
       61 cctgcagcac ttcgggtccg gcggtcagca gctcccgcaa cggcaggtaa gccgccgagt
      121 cgtgcgccgc gcaggcggcc tgcagcaggt ggctcttgcc actgccgtcc ggtccccaga
      181 cccacagggc cggtgcggtc gccgccggct ccgccagcgc cgcgaccacc gcgccgttcg
      241 gccccggttc gaagctgtcg aagacggcaa agtcgcgcag ccggacggcg agcggcagct
      301 ggcgcatcag gcgttggcgg cccgcgcgcg gccgctccag cggatcacgt agtcgcagcc
      361 gctgacgatg gtggtcgcca gcaccgccca gcccaggccg tcgatgaggc cgcgcggcgg
      421 cagcgcgaac accagcgcgg ccaggctcag caggatgtag agcagctgga ggaaggagtt
      481 gcacttgctg atgatcgtgg gctcggccgt gaagtccccg acacgccact ggtagacgcc
      541 ggcgccggcc acgatcacca ggtcgcgcag gaccaccgcc gccagcagcc agcccggcac
      601 gtagcccgcc gcccacagcg tcacgaaggc ggtcaccagc aggagcttgt ccgccgccgg
      661 atccaggaag ccgccgaggc ggctttgcca gtggaagcgc ttggccagga agccgtccag
      721 ggcgtcggtc aacccggcga tgaagaacat caccagtgcg gccgggtagc gcccctcgac
      781 gatcgaccag ccgatcggca cggtgagcgc gattcgcaac acgcacagca ggttggggat
      841 gtcccgggct ctcacctggc cacgcgatag ttgagatcgt gggcgagatt gtcgcggtcg
      901 ggcctcagca agccgccgag ccgcacggcc tcgtcgagcg cggatgcctc gccgcgcacc
      961 gccacgcgga acacgacgcg gtcgccgacg accgattcca cgcccaccga ctcgatcgcc
     1021 gtcagccggc ccaggaaggt gctgacgcga ccgtaatcct cgaccgttgc cacgcccgag
     1081 accgccacct ggaggatgcg gccggtgccc gacgcgacca ccgcgaagcg agccgcgaag
     1141 cgatccgcga gccggcccac gctgtccggg atcccgccgc tccagcgctc ggccccgtcc
     1201 gggccatgga gagtccagcg accgtggccc aggtcgcccg ccgatagcct gccgaccagc
     1261 accgcgtcca cgccgtagcg ctccgatgcg gccaggatgg aatcctcgaa accgccccag
     1321 acctctgcga aggaggcccg cgcgcggtcc tccgcgtcca tcagcggaaa caccaccggg
     1381 ataccgcgct cggcggcggc ctcgagcacc tcggcgcgga tcgccgcttc ctcgctcgac
     1441 tccgcgccgg aggccaccac gaagcgcctg ccgccgccgg catcgaccgc aatccacagc
     1501 aacgtgctcg gccgttcggg accccatacc ggctgctcca gccggccgag ctcgcgctcc
     1561 agcgcggcgc cgtcgaacgc aacccacagg cgatccggcg cgggctggcg gaactgctgg
     1621 gcgtaggctg cggcattgtc cagcaatttc gcgacttccg gccggccggc ggcgtcccgc
     1681 cgtcccgtga tgcgcaccag cacctggccg agcgccgcgc ggaacgcctc gtcgcgcccg
     1741 tcggtcagct gcgcctcggc ctggtagagg ccctccacgc gggcaccagc agcgccatgc
     1801 cggcggccag cgccgccgcg actaggggat gaatcgattt ggtcactttc cgggttatcc
     1861 tgccgcgctt ggaagcgtcg gataaaatac cacgctaccg ccccctgggc cgccaaactg
     1921 gatgcaatcc cgatgaagga cacatcgtcg ctgacgtaca aggacgccgg cgtggacatc
     1981 gatgccggca acgcgctggt cgagcgcatc aagccgttcg tggcggccac gcgccggccg
     2041 ggcgtgctgt cgggcatcgg cggcttcggc ggcctgttcg agattccgtc cggctaccgg
     2101 cgcccggtgc tggtcgccgg caccgacggc gtgggcacca agctgaagct ggcgatcgac
     2161 accggccggc acggcggcgt cggcatcgac ctggtcgcga tgtgcgccaa cgacgtcatc
     2221 gtgcagggtg ccgagccgct gttcttcctc gactactacg ccaccggccg gctcgacgtg
     2281 gacagcgctg cggccgtcat cgagggcatc gccgagggct gccggcaggc cggggcggcg
     2341 ctgctcggcg gcgagaccgc cgagctgccc ggcttctacg cgggcaagga ctacgacctg
     2401 gccggcttct gcgtcggcat cgtcgaatac gagcgcatca tcgacggcag ccgcgtcgcg
     2461 ccgggcgacg cgatcgtcgg catcgcctcc tccggcctgc actcgaacgg ctattcgctg
     2521 gcccgcaagg tgctggagcg cagcggtgcc ccgccggaca tgctgctcga cggcgtgccg
     2581 ctgatcgagc ggctgctggc gcccacccgc atctacgtga agccgctgct ggccctgctg
     2641 gcggcggtgg aagtgcacgc catggcccac atcaccggcg gcggcctgcc ggaaaacctg
     2701 ccgcgcgtgc tcccagaggg cgcggccgcg cgcatcgagc tggcgagctg gcggcgcccg
     2761 gcggtgttcg actggctgca ggccgagggc ggcatcgccg acgcggagct gtatcgaacc
     2821 tttaactgcg gcctcggcat ggccgtggtg ttgcccgcag cggatgcgga ccgcgccgtc
     2881 gcgctgctga acgagcacgg cgagcaggcc tggctcgccg ggcgcatcga ggcgggcgag
     2941 cgccgggtgg tgctcgccgg atgagccgcc ggccgctgcc cgtcgcggta ctcgtctcgg
     3001 gcttcggcag caacctgcag gccttgctcg atgccgccga cgccggtcga ctgaacgtcg
     3061 agttccggct ggtggccagc gaccggcccg aggcgccggg cctcaagcgc gcgcggcgcg
     3121 ccgggctggc caccgcggcg ttgccgccgg acgactatgc cgaccgcacg gcctggaacg
     3181 acgccatggc aacagcgctg cgcgattccg gcgccgagct ggtggtgctc gccggcttca
     3241 tgcgcgtgct gggtgctgag ctggtgcgcg cctggcaggg ccgcatgctc aacgtacacc
     3301 cctcgctgct gccactgcac cgcggcctgc acacccaccg tcgcgccctg caggccggcg
     3361 accgcatgca cggtaccagc atccatttcg tcaccgagga actggatggt ggcccggtga
     3421 tcctgcaggc gcgcgtgccg gtgctgccgg gcgacaccga ggagcgcctc accgcgcgcg
     3481 tccaggcgcg cgagcacgag gtctacccgc gggtggtgca gtggatcgct gacggctggc
     3541 tcgagtggcg cgacggcggg ccctggctgt tcggccgccg gctcgccgag ccggtcgtcg
     3601 tctagccaaa accggggtca gacaccattt tccggtcgac cgcgggcgcg cgccatcagc
     3661 gcttcgttcc ggtcgaccgc ggggccgtgc cgccatgggc gcgcttcgct cggcgaactc
     3721 taagctcgct cagcacgtcc atggcgtccc acccccgctg ccttgtcgac aacgccggcg
     3781 tcgaccgttg cctggctcga cgaggtcgcc ttggggttgg ccgaccaccg gctggcgcag
     3841 cgtttgtcgc tgccgtagcg ggtgtggggt gtcataggcc ggccgagcga gtttttcctt
     3901 gtccgagcgc aggccggcct atggcgccgc gcgcccgcgg tcgactggca accgaaggcc
     3961 ccgcgcgcgg gcaaacggaa atgggtctct gaccccggtt tttcaggtct tgggcagggt
     4021 gacgccgcgc tggccctggt acttgccgcc gcgatcggcg tacgagacct cgcattcctc
     4081 gtccgactcc aggaacagca tctgggccac gccctcgttg gcgtagatct tggcgggcag
     4141 cggcgtggtg ttggagaact ccagggtgac gtggccctcc cactccggct ccagcggcgt
     4201 gacgttgacg atgatgccgc atcgcgcata ggtgctcttg cccaggcaga tcgtcagcac
     4261 ctggcgcggg atgcggaaat attccacggt gcgcgccagc gcgaaggagt tcggtgggat
     4321 gatgcagacc gcgccgctga cgtccacgaa actcgactcg tcaaaggcct tcggatcgac
     4381 caccgccgag ttgatattgg tgaagatctt gaaatcgggc gcgcagcgca cgtcgtagcc
     4441 gtagctggac gtgccgtaac tgacgacctt gcgaccgtcc actgcccgga cctggccggg
     4501 ttcgaagggc tcgatcatgc cgtgctgctc cgccatgcgg cggatccagc gatcagactt
     4561 gatgctcaat caatcgtcct cgaagctgat cgtcgggaat ttcgcgcccg gcagctcgcc
     4621 gccggccgcc agccgcgcgg acatgcgccg tgcaatgtcc aggtaggcgc gtgccgccgg
     4681 gccatccgga tcggacacca ccgaaggctg accggcatcc gcttcctcgc ggatgtggat
     4741 gtcgagcggc aggttgccga gcagcggcac gccgtattgc tccgccatcc gctcaccgcc
     4801 gccggagccg aagatgggtt cctggtggcc gcaattcgag cagacgtgca ggctcatgtt
     4861 ctccacgatg ccgagcaccg gcaccttcac cttctcgaac atcctgaggc ccttgcgggc
     4921 atccagcagg gcgatgtcct gcggcgtggt gacgatgacg gcgccgctga ccgggacccg
     4981 ctgcgacagg gtcagctgga tgtcgccggt accgggcggc atgtcgacga tcaggtagtc
     5041 gatgtcgccc cagtgggtct cgccgagcag ctgctgcagg gcctgggtga ccatcggccc
     5101 gcgccagatc atcggctggt cctcatcgat caggaagccg atcgacatgc aggcgatgcc
     5161 gaaggcctcc agcgggtgga tccgcttgcc gtccgtgctg gtcggccggc ggcccgacag
     5221 gccgagcatg cgcggctggc tggggccgta gatgtcggcg tcgaggatac ccaccttggc
     5281 gccctcggag gccagcgcca gcgccaggtt caccgcggtc gtggacttgc ccacgccgcc
     5341 cttgcccgag gcgacggcga tgatgttgcg aatgccggcc agcgggctga ggccgggctg
     5401 gaccgagcgg gtctcgacat cccactggat ctcgaccttg accgagctca cccccggcag
     5461 cgaccccagc agtccggtga actccgcctg cagctcgcgc tcgtagcggt cggccgggaa
     5521 gcccaggcgc aggaccacgc tgagcacacc gcccttgagc gcgaggtcct tgatcgcccc
     5581 ggctgagacc aggtcctgct cgatgacagg gtcgatccgc tgcgccagca gcgcgcgcgc
     5641 ggtgtcaatg gttgcctcgg tcatgcctct ccccgggggt gtcaaaagta ggctccagga
     5701 gccgcgcatt gtagcccatg cgcggaccgg cggcagcgac tcttggacgg aaaaccgcgg
     5761 tgcgtgtaac ttagggcgga tgtggtcgga cctcccccct gacaacagcc ttctcgaccg
     5821 accgagggga ctgtcatgag cctgatcggt ggcttcgttg ccgaccgcct ggttgcgggc
     5881 attcgcggcc cggcggacct cgagtcggcg gccggcaaga aggccattgc gcggctgcgc
     5941 aaggtgggcg gcccggcggt gaccaagctg gtggcggagc tcgagagtgc ggacaaggag
     6001 cgcgcagcgg cgctggcccg cgtgctcggc acgctgattt ccgtgcgcac cctgccggag
     6061 gcggccgacg ccctgcgcca caccaacgcc cgcggactga agggtctggt ctgggcgctg
     6121 tccaacagca aggactacaa ccccaacaag ctgctcgact acctgtccga ggaaggcatg
     6181 ccgcgcgcgg cggtgctgga gatcctcgag gcgcagaagg accggctgga gttccggccg
     6241 gtgctgaacc gcgcctacca ggtggaaagc accgaaaagg cggcgctgtt caagctgctc
     6301 ggtgaaatgg ccgacgacga gatgctgccg gagctcgtca gccgcctgtc gggcaaggac
     6361 gtcgcggcca aggtgcacat catggacatc ctgtcgcgct tcgaccggcc cgaagtgcgc
     6421 catgccctta ccgaggagct cggcagcgac aacaagctgg tgcgccagac cgcgctcgcc
     6481 gcgctcggcc gcctcggcgg cgagctggac atcgcccggc tgtgcaagct gctgatggac
     6541 ccggacatca gcgtccagaa tcgggccgtc gacgtcatca tcaagcagcg ccacccggac
     6601 acgctcaggc atctcgtcga ggtgctcaag agtgagtcgg attttgcccg ccgcgccgcg
     6661 gtcgaggtac tcaacgagat cggcgaccag gaatccatca aggacctgct cagcgcgctg
     6721 agcgacgacg actggtgggt gcgtgcgcgc gccaccgacg cgcttgcgcg gatcggcggc
     6781 ccgcgagtca tcgacgcggt gctcgggctg atcaacgacg aggacgaaac cgtgcggcgc
     6841 tcggcggtgg agatcctgaa caccaccaag gacgagcgcg ccgtgcagca cctcatcaag
     6901 gccaccaccg accccgactg gtgggtgcgg gagcggtccg ccgacgcgct cgccgaaatc
     6961 ggcgacgtgc gcgcggtacc ggcgctgatc aagatgctgg gaggcgagcc gcgctccgtg
     7021 ccggcggcgg tccgggcgct cgccaagctg gccggcggcg acgccgtggc ccacctgaag
     7081 ccgctgctgg agcacaagga tccgcaggtt cgcgtcgagg tgatggcggc gctggcggag
     7141 accgccgatc cgtcggccgc ggagtccatc atccagcgca tcgccaagga gggttcctcc
     7201 aaggagacga ccgtcagcga ggcggccgcc tcggcgatcc agcggctcag cgagcggatg
     7261 ggcacggcca ccacctcggg gacgctggcg tcgggcacgg gcggagcagc agcagcagcc
     7321 tcgctggccg acaccaagga cctgcgggaa ttgctcaagg ccgcggccca gcagtccgac
     7381 gcgctggaca tcaccaagct cgagcccggc gacaccatcg agggccgcta caagtacatc
     7441 cgcaagatcg gcaagggcgc cttcggcacc gtgttgctgg tcgaggacat ggtggtggac
     7501 gagcggctgg tgctgaagtt cctgaacccg ggcgtggcgg ccgacgagga gatgatgaag
     7561 cgcttcgtcc acgagctgcg ctactcccgc aagatcacgc acccgaacgt catccgcatc
     7621 tacgacttca tcgcgctcgg cggcctgtac gccatctcga tggagtactt cgcctcgcac
     7681 ccgttgtcgg acgagctcaa ggacgagaag ccgctgccgc tgaaacggac cctgcaatac
     7741 ggcatggaca tcgccacggg catgcaggtg gcccaccagg cgggcgtcat ccatcgcgac
     7801 ctcaagccgg ccaacgtgct catcaacgac gacagcctgg tgaaggtcgt ggacttcggc
     7861 gtggcggccg cgcgcgccag cggcgacaca cagctcaccc gcacgggctt tgtgataggc
     7921 tcgcccaagt acatggcgcc ggagcagatt ctcggcaaga aggtcgacga gaaggccgac
     7981 gtgtacagcc tgggcgtgat gctttacgag atgctgaccg ggttcgcgcc ctatacgcgt
     8041 ggagaccaca tgtccgtgat gtaccagcat gtgcagggca aggccaagcg ggtgcgcgag
     8101 ctgaacccga acgtcccgca ggagctgtcc gacctggtgg cgcgctgcat ggcggtggac
     8161 aagagcaagc gctacccctc gatggacgag ctgcgccatg cgctggcgcc cttcgtcggc
     8221 acggagacct aggccctggc gagaatagac gccttcctgc agctcgccat ggcccagggc
     8281 tgctcggaca tccaccttgc ggtcggcgtg ccgccgatgc tgcgcctgcg cggcgacctg
     8341 atgccgatca agttccgcga cctgtcgccg tccgagctgg aaagttacgt caacgagatc
     8401 ctgaccccgg cgcagaagaa gaccttcagc ggcgggcacg atctcgactt ttcctacgtc
     8461 agcggcgacg gcgggcgttt tcgcgtcaac ctgttccgca aggacaccgg cccgggcgcc
     8521 gtgtttcgct tcatcccggc cgaggtgccc acgctggagt cgctcgggct gccgccgatc
     8581 attcgccagc tgtgcgacta ccaccagggc atggtgctgg tgacgggcgc caccggcacg
     8641 ggcaagtcca ccaccctcgc cgcgatgatc gatttcctca actcgacgcg caagatgaac
     8701 atcatcagcc tcgaagaccc gatcgagttc gtgcacaagt gcaagaattc gcaggtcgtg
     8761 cagcgcgaac tcggcactca cctgacgagt ttctcggccg gcgtgcgcgc ggcgctgcgc
     8821 gaggatccgg acgtcatcct ggtcggcgag atgcgcgacg ccgcgaccat cagcatggca
     8881 atgaccgccg cggaaaccgg gcacctcgtg ctcggcacgc tgcacaccac cggtgcggtc
     8941 aagaccatcg accggatcat cgacgcgctg ccggcgcagc aacgcgagat gaccaagagc
     9001 ttcctgtcgc agagcctggt ggccgtggtg acgcaggtgc tggtgaagac cgcggacggg
     9061 caacgccgca aggccattca cgagatcctg gtgcagaaca aggccatcgc caagctcatc
     9121 atgagcgacc agacccacct gattccctcg cagctgcaga ccggcaagga ggcgggcatg
     9181 cagctgatgg accaggcgct gctggaggcc atccagcggc gcgagatcga cccggacgac
     9241 gcctatcgct tcaccaccga caagaagcag ttcgcgcgct tcgtcaccga caccggcatc
     9301 ctgccgaaac tggacctcgc ggaatgaccg gcaccaaccc cacgctgacc caacgcaact
     9361 cgcaactggt ggaccggttg ctgcgcgcca tggtggagcg cggcggctcg gacctgcacc
     9421 tgatcgcggg caatccgccc cgcttccgcc tgcatggcga cctccaggtg ctggagcaga
     9481 ccttcctcga ccagcaggaa ctggcccaga acctgcaggc catcgccaca ccgctcgcgg
     9541 tcgcgacttt cgagaaggaa gagcaggcgg atttcgccta catgatccag ggcgtgtcgc
     9601 gattccgggt caacgtcttc cggcacctcg gcggcatggg cgccgtgatg cgggccattc
     9661 cctccgaagc ggtcagcctg gagtcgctca agctgccgga agtcgtgcgc agtctttgcc
     9721 agcaccgcca ggggatgatc ctggtcaccg gcaagaccgg ctcgggcaag tccacgaccc
     9781 tggcggcgat gatcaacgag atcaacctgc gtcgcaaagg gcacatcatc accatcgagg
     9841 acccgatcga attcgtccac cagcggcagg gctgcctgat cagccagcgc gaggtgggca
     9901 accacacgcc gaccttctcc gaggcgctga tctcggcgct gcgcgaggac ccggacgtcg
     9961 tgctggtcgg cgagttgcgt gacctcgaga cgatgagcac ggccatcacg gccgcggaaa
    10021 tgggcatcct ggtgatgggc accctgcaca ccaacggcgc ggccgccacg gtggaccgca
    10081 tcgtgcatgc cttcccgccg gagaagcagc cgcacgtgcg caccatgttg tccacctcgc
    10141 tgcgcggtgt gatctcgcag cagctctgcc ggcgcaagac gggtgacggc cggatcgcgg
    10201 cgctggagat cctgatcaac accccggcgg tgtcgaacct gctgcgccag ggcaagctgg
    10261 accagctcga gacggccatg cagtccggcg ctaacgtcgg catgcgcacc atggactcgg
    10321 cgctgcagga gctgctggac cggcgcttca ttacgccgcg cgaggcctac gacaaggcgc
    10381 tgtccaagga tcgcttccgc aagctggtcg aacaatccga gccgccgccg ctgcggggcg
    10441 gggccaagcc tccggatatg gcataatttc gggtttgacg ccgcgtcccg gcggaaaggc
    10501 cccatgagcg aaagccgcga aatcctggtc accagcgccc tgccctacgc gaacgggccg
    10561 ctgcatctcg gccacctggt ggaatacatc cagaccgaca tctgggtgcg tttccagcgc
    10621 atgcgcgggc accactgcct gtatgtctgc gccagcgacg cgcacggcac gccgatcatg
    10681 ctcaaggccc gccaggacgg catcacgccg gagcagctga tccgcgaggt ccatgccggg
    10741 cagctcgagg acttcgccgg cttccacatc gccttcgaca actaccacac cacgcattcg
    10801 ccggagaatc gcgcgctggt ggaggaaatc ttccacgcgc tggaagccgc cgggcacatc
    10861 gagacgcgca gcatccgcca gtcctacgac gagcaggaag gcatgttcct gccggaccgt
    10921 ttcgtgcgcg gcacctgccc ccgctgcggc gccgccgacc agtacggcga cagctgcgag
    10981 gtctgcggcg ccacctaccg tccgtccgag ctgctggaac cggtctcggt gctgtccggc
    11041 acccgcccgg tggagcggga atcggagcac ctgttcttcc gcctcggcga cttcgaggac
    11101 atgctcaagc gatggatgaa cgagggccgc ctgcacgaat ccatgcgcaa caagctggag
    11161 gaatggttcg aggccggcct gcaggactgg gacatcagcc gtgatgcgcc ctatttcggc
    11221 ttcgagattc cgggccggcc gggcaagtac ttctatgtct ggctggacgc gccgatcggc
    11281 tacatcgcca gtttccgcca gctctgcgac cgccgtcccg acctcgactt cgacgcctgg
    11341 tggcgccccg gcggcgacaa ggagctctac cacttcatcg gcaaggacat cgtctacttc
    11401 cactcgctgt tctggccggc ggtgctgcac gggtccggct tccgcacccc gaccgcggtg
    11461 ttcgcgcacg gcttcctgac ggtgaacggc gagaagatgt ccaagtcgcg cggcaccttc
    11521 atccgggcgc gtacctggct ggagcacctc gacccggagg cgttgcgtta ctactacgcg
    11581 accaagctgg gaccgggcac cgaggacatc gacctcaacc tgcaggactt cgtgcagcgc
    11641 gtgaactcgg acctggtcgg caaactggtc aatatcgcca gccgctgtgc cggcttcatt
    11701 caccgcgtgg cggagggccg gctggctgcg acgctgcccg agccggccct gcaccgggag
    11761 atggcggcgg ccggtcccgc gatcgccgag tgcttcgagc agcgccgtta cggccaggcc
    11821 gttcgccagg tcatggcact cgccgaccgg gccaaccagt acatcgaccg cgagaagccg
    11881 tggcagatgg cccgcgagcc ggggcgcgag gccgaggtgc agtccgtctg cacccaggga
    11941 ctgaacgcct tccgtagcct ggtcacctgg ctcaagcccg tgctgccgcg cctggccgag
    12001 gcggccgagc agttcctgca atgcccgccg ctggcctggg acgatgcggg cacgccgctg
    12061 ctggaccatc gcatcgggcc gttcgaaccg ctgatgcagc gggtcgatcc ggccgcggtg
    12121 cagggactgc tggaggcctc gcgggacagc ctcgagaaga agcccgcgcc cggggcggcc
    12181 agggctgcgc ccaaacaggc ggaggaactc cccgccgaga tcggcatcga agagttcatg
    12241 aaagtcgacc tgcgggtcgc acgcattctc gaggccgact acgtcgatgg cgccgacaag
    12301 ctgctcaagc tggtgctgga cgtaggcaat ggccggcgcc aggtgttctc gggcatccgc
    12361 tccgcctacc gcgccgagga cctgcccggc aggctcacgg tggtggtggc gaacctcaag
    12421 ccgcgccaga tgcgcttcgg cctgtccgaa ggcatggtgc tggcggccgc gggcggcgac
    12481 ggcattttcc tcgtcgcgcc ggacagcggc gccgagcccg gcatgcgggt cacctgacac
    12541 ggcgcgcttt cgaaccaggc gggcatgaaa gaactcggac tcatcctcgt ggcgacggtg
    12601 ctggtgaaca acttcgtgct ggtgaagttt ctcggcctgt gcccgttcat gggcgtgtcc
    12661 cgcaagctcg agacggccac cggcatggcg ctcgccacga ccttcgtgct gacgctgtcc
    12721 tcggtgtgca gttacctggt acaggagtgg ctgctggcgc cgctggggct ggagtacctg
    12781 cgcaccatcg ccttcatcct ggtgatcgcc ggcgtggtcc agttcaccga gatggtggtg
    12841 cacaagacca gccccctgct ctaccaggtc cttggcatct acctgccgct gatcaccacc
    12901 aattgcgccg tgctgggcgt ggccctgctg aacgtgcagg agagccacgg cttcctcgag
    12961 tccttcgtgt acggattcgg cgccgcgctc ggtttctccc tcgtgctggt gttgtttgcc
    13021 ggcgtacgcg agcgggtggc ggccgccgat gtgccggtgg ccttccgcgg cgcctccatc
    13081 gcgctggtga ccgccgggct gatgtcgctt gcgttcatgg gcttcgccgg cctggtgcgc
    13141 ggttgagcgg ggccggcgaa tgatctcggc agtcgtcgtc atcgccgggc tcgccaccct
    13201 gttcgggctg gcgctcggtt tcgccgcggt gcgcttccgc accgaggggg acccggtgac
    13261 cgatcggatc gacgcactgc tgccgcagac ccagtgcggc cagtgcagct tcgccggctg
    13321 ccgaccctat gcggaggcga tcgccgccgg cgaggcggac atcaatcgct gcccgcccgg
    13381 cggcgaggcg accattcgcg cgctggccga cctgctggag cgcgatccca agccgctcga
    13441 cccggagagc ggtgaaatca ggccgcgcac cgtcgccgtg atcgacgaac ccctctgcat
    13501 cggctgcaca ctgtgcatcc aggcctgccc ggtggacgcc atcctcggcg ccggcaagca
    13561 gatgcacacc gtgatcacca gcgaatgcac cggctgcgag ctgtgcgtgg cgccctgccc
    13621 ggtggactgc atcgccatgg tcgaggaacc ggtcacgctc aagacctgga aatggccgcg
    13681 tcccgacgac cccgcgcagc cggcggagcg ccggtgagca agccgcggct ctggcatttc
    13741 cacggcggcc tgaagctcga tcctcgcacc gcgaggccgg catccgtccc gctcggccag
    13801 gtgccggtgc cggcgcggct ggtgctgccg ctgtcgcagc acgtcggcca gcccgccgaa
    13861 ccggtggtga cgatcggcga gcgcgtcctg aagggccagc ccgtcgctcg tcccagcggc
    13921 tacatcgccg ccaaggtgca tgcctccagc tccggcacgg tggtggagat cggcgaccat
    13981 cccgtgcccc acccgagcgg cctgtcggcg ccctgcatcg tcatcgagac cgacggcctg
    14041 gacgaggcct gggaaggtta cgagccgctc gccgaattcg cgcagctcaa cagcaacgaa
    14101 ctgcgcgcgc gggtgcgcgc tgccggcgtc gtcggccttg gcggcgccgc ctttcccacc
    14161 gcggtgaagc tgggttcggg caccgggctg aagctgcttg ttctcaacgc ctctgaatgc
    14221 gagccctaca tctgctgcga cgagctgttg atccgcgagc gaccggagga agttctcacc
    14281 ggggcgcggg tgctgatgcg tgcgctcggc atcgatcgct gctgcatcgc catcgaggaa
    14341 gacaagccgg aagcgaagcg tgtgctgctc gacgccatcc aggccgccgg cgagacccgc
    14401 atcgaggtgg tcgccgtgcc ggcggtctat ccggaaggcg gcgagcgcca gctcatcaag
    14461 gtgctcacgg gcgaggaagt gccggcacgc ggcgtgccgc cggacatcgg ctacctgtgc
    14521 cagaacgtcg gcacggcggc cgccgtggcg cgcgccgtgg tgcgcggcga gccgttgatc
    14581 tcgcgcatcg tcacggtcac cggcgcgggt gtcgcccggc cgcagaatgt cgaggtgcgg
    14641 ctcggcacgc cggtcgcgga cctggtgcgt gcctgcggcg gctataccga tgacgccgcc
    14701 tacctgctga tgggtggcgc gatgatgggc tttccgctgg cggacgacac gctgccggtc
    14761 atcaagggta tgaactgcgt ggtggtcgcc tcgcgcgagg agatccggcc gcgcgcgccg
    14821 gccatgccct gcatccgctg cggcgagtgc gccgaggtct gccccgccca gctcctgccg
    14881 cagcagctct actggcacgc acgggccggc gagctccacc ggctcgacga gtatcacctg
    14941 ttcgactgca tcgagtgcgg ctgctgcgat gtggcgtgcc cgagccagct gccgctcacg
    15001 cagtactttc gctacgccaa gaccgagctg tggtcacgcg gccgggaccg ggcccgctcg
    15061 gacctggccc gcgagcgctt cgaggcccgt cgccagcggc tcgacgcgga gcaggaggcc
    15121 cgcaagcgtc gtctcgagga gaagacccgc gccgccgacc aggcgcgcgg cgacgagcag
    15181 gcgcgcaagg caatgatcga cgaggccctg gagcgggtac aggcacgacg cgagcaggac
    15241 caggacggac gcgcggagcg cgaggagtga agttcgagcg caccgcgccg ccgcacgtca
    15301 ggccggccac cagcgtcaca cgggtgatgg gcgaagtgct gctggcactg gtgcccgcga
    15361 ccgtcgcatg ggtgtggttc ttcgggccgg ggctgctcta caacatggcg gtggccgcgg
    15421 ccgtggcgct ggcctgtgaa gccggcgtgc tggccttgcg ccggcgaccg gtgggtccgc
    15481 aattgctcga cctcagcgct ctggtgacgg ccatgctgct ggccttctgc ctgccgccgt
    15541 tgacgccttg gtggatcacc gccaccggcg ccgcgttcgc catcgtgttc gccaagcagc
    15601 tctacggcgg gctcggctac aaccccttca accccgccat ggccggctat gtcgtgctgc
    15661 tgatctcctt cccggagcag atgacgcgct ggctgccgcc gctgggcgac tccctcggcc
    15721 tggccgccgc ctcgcttgat ccctggcaga cgctgcgttt caccttgacc ggcctgctgc
    15781 cggacggcct gtcgtgggat gccgtcagcg gtgcgacgcc gctggacgac ctgcggacgc
    15841 agctgtcgat ggcgcgcacg gtcgaggagg tgcgcgcggg cgcgctgtac ggcagcctcg
    15901 gggcacgcgg ctgggagtgg accgccaact ggatcgcgct gggcgggctc tacctgctgt
    15961 ggcgcaagat cattcgctgg cagatcccgg tcgcggtgat cctcggcgtg tgcgtacccg
    16021 ccgcgttctt ctggctcatc gacccgggcg cccatccggc gccgaccttc cacctgttca
    16081 gcggcgccac cttgctgtgc gccttcttca tcgccaccga cccggtctca gccgccacca
    16141 gccgctcggg caggctggtc tacggcttcg gcatcggcgt gctgatcttc gtcgtccgca
    16201 gctggggcgg atatcccgac ggcgtcgcct tcgccgtctt gctgatgaac atggcggtgc
    16261 cggccatcga tcatttcacg cggccaagga cctatgggca tgcgggctga gatcgtccgc
    16321 agcatgttgc gcgccgccgg catcctggcg ggcttcgccg tcttcggcgc cctgctcgtc
    16381 gccgtcacct gggactcgac cgaggaacgc atcgccgcga acgagcgcgc cttcctgctg
    16441 cgcagtctcg cggacgtgct gccggacgac ggctacgaca atgccgtcca cgaggacgtc
    16501 gtcatggtca cggacgccgc gctgctgggc acgtcggcgc cgctcagcgt ctatcgcgcc
    16561 cggctgcgca accggccggt ggcgctggtg ctgaccccgg tcgcgcccgg cggctacagc
    16621 gggccgatca agctgctggt cggcgtactc gccgacggca cggtcagcgg tgtgcgcgtc
    16681 gtcgcacacc gggaaacgcc cggcctgggc gacaagatcg agctcgagcg agacgactgg
    16741 atccttgatt tcgacgggcg ccgcatcggc cagcccccgc gtgaacgctg ggcggtgcgc
    16801 cgcgacgacg gcgtgttcga ccagttcacg ggcgccacca ttacggcgcg cgccgtggtc
    16861 acggccgtgc gtgacgcact gctatacttc gaggcccatc gcgacgagct gttcgcggcg
    16921 ccggccggcg cgacactggc gccgccggcg caacgtgacg acgaggacga gcaatgagcg
    16981 tgacccccgc ggaagtggtg cgcaccgggc tgtggaccaa caatgccggc ctggtccagc
    17041 tgctcgggct gtgcccgctg ctggcggtca gcaccagcct ggtgaacggc ctggggctgg
    17101 ggctcgcgac cacgctggtg ctggtcgcct ccaacctgac ggtgtcgatg atccgcgaat
    17161 tcgtgcggcc ggaaatccgc ataccggtgt tcgtactggt gatcgcatcc ttcgtgaccg
    17221 cggtcgagct caccatgaac gcctggttcc atgagctgta ccgcgtgctg ggcatcttca
    17281 ttccgctgat cgtcaccaat tgcgccatca tcggccgtgc cgaggctttc gcctcgcgcc
    17341 agccgccggg ccttgccgcg ctggacggcc tgtcgatggg tgtcggcttc accggcgtgc
    17401 tggtcacgct gggcgcggcg cgcgagctga tcggccaggg cacgctgttc gccggcgccc
    17461 acatgatgtt cggtgagggc gcccgcgcga tcgagctgca gttcgccgac ggcggcttcc
    17521 tgctcgcgct gctgccgccg ggcgcctttc tcgggctcgg cctgatggtg gcggcgaaga
    17581 acttcctcga tgcgcgtctc gagcgccgtc ccgcgccggc gccggcgacg cctgcggcgg
    17641 cggccggcga gacggcctga ggcccgggcg catcgtgaac aacaccaggc gtcgggaact
    17701 gtacgagcgt ctccgggcgg ccaacccggc gcccaccacg gagctgaact accgcagccc
    17761 ctttgagctg ctggtggccg tggtgttgtc ggcacag
//
LOCUS       DAOMPG010000016        17692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1408_length_17692_cov_5.308953, whole genome shotgun sequence.
ACCESSION   DAOMPG010000016 DAOMPG010000000
VERSION     DAOMPG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_17692_cov_5.308953"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..2288
                     /gene="uvrD"
                     /locus_tag="PLS34_01635"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             132..2288
                     /gene="uvrD"
                     /locus_tag="PLS34_01635"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HRP86205.1"
                     /translation="MDITPILDPLNDAQREAVTAPSDAPVLVLAGAGSGKTRVLVHRI
                     AWLVQVEGVSPWGVLAVTFTNKAAGEMRSRIEELLGMPAGNLWVGTFHGLAHRLLRLH
                     WREAKLPQTFTVLDSEDQLRLIRRLLKALELDENRWVPREVQWFINARKDEGLRAAHL
                     DAGDDPNRRMFIRIYEAYQAQCEQSGLVDFAELLLRAFELWRENPELLDHYRRRFRHL
                     LVDEFQDTNAIQYAWLRLLAGPEGKPFIVGDDDQSIYGWRGARVEHIRRFRKDFPGTR
                     LVRLEQNYRSTGNILNAANALIANNRERLGKKLWTEGHAGEPIRLYAAYNERDEAEFV
                     VGRIRAAIEQGAANSDMAVLYRSNAQSRVFEEALIQARIPYRVYGGLRFFERAEIKDA
                     LAYLRLIANRDDDSAFERVVNLPTRGIGARTLEALRDYARANATSMWRAAGALVTDGL
                     SARAGQALHGFLALIEALDRDTRALELHEQVDHVIQASGLIAHHGKEKGERGEARIEN
                     LEELVSAARGFEPEPDSEMRLLDEFLAHAALEAGEGQGGAWEDCVQMMTLHSAKGLEF
                     PIVFLAGMEDGLFPHKRSLLDADGLEEERRLAYVGTTRAMRELYLSYAEQRRLHGVDN
                     YGIPSRFIGELPAELVEEIRPAVRVSRPAAAPSGRFRDQAPGGISLGQRVRHGQFGEG
                     VVLNHEGHGSHARVQVNFEQAGPKWLVLAYARLEVM"
     gene            2324..3736
                     /gene="trkA"
                     /locus_tag="PLS34_01640"
     CDS             2324..3736
                     /gene="trkA"
                     /locus_tag="PLS34_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRP86206.1"
                     /translation="MKILILGAGTVGSTAAESLSEENEITVVDLKGQHLRELQDRLDI
                     RTVEGHASHPRVLEQAGAADADILVALTDSDETNMIACQIAYSLYRTPVRIARIRATE
                     YTSRKELFTNKTIPVDTIISPEQLVTEHIEQLIHYPGAHQVLDFAEGRVRLVGLMAKA
                     GGPLIGQELRELRRHIPHTDARVAAIYRRSTIPPVGNEEPLYETILPEGDTVIRPNDL
                     VFFLAARKDIRVMMSELLKLEHPARSIIIAGGGHIGFELARRLENTNRVKVIERDHAR
                     ARYIAERLRHAVVLTGDAVDEELLAEENIDKVDVFCALTSAEEANILSSFLAKRMGAS
                     RVIALINRPSYAKLVEDQYVDIAVSPQEITLSALLAKVRGMRLDVGQVHALRSGTAEA
                     IEAVAHGDSRTSKVVGKAIEDVPLPPGATIGAIVRDEEVLMAHHDTEIRSEDHVILFL
                     TDRSQIPAVEKLFQVGVTYT"
     gene            3753..5282
                     /locus_tag="PLS34_01645"
     CDS             3753..5282
                     /locus_tag="PLS34_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRP86207.1"
                     /translation="MNGLVVLRIIGLLLSLFSLTMLPPVVVSLLYNDGAWPAFVEAFF
                     IFLAAGLALFLPLRRRRAELRIRDGFLVVAGFWVVLGFAGAVPLVLVEIPIMSITDAV
                     FEAVSGFTTTGATVLVGLDTLPHSVLWYRQQSHWIGGMGIIVLAVAILPVLGVGGMQL
                     YRAETPGPMKDTKLTPRITETAKALWITYVGLTAICAVSYRLAGMNWFDAIGHAFSSI
                     ATAGFSTHDASIAYFDSGLIEWLTALFMFLGATSFALHFYAMRRLMPDIHLTTRGGVP
                     RFSWPHRRKVSLKASIGGYFNDPEFRAFMAIQAGLIAIIAAYLTLKGHHAPGESLTKA
                     VFQSVSFGTTTGYTSTDYSAWPGALPTLLILASFIGGCAGSTAGGMKVLRWLLIFKQG
                     WRELARLLHPNAVMMVRLGERGVPSRVTEAIWGFFSVYVVVFCILMAVMMTIGLDQVT
                     AFSAVAATLNNLGPGLGEVSITFGTVPDTAKWVGVLGMILGRLEIFTLLVLLTPEFWR
                     R"
     gene            5282..5605
                     /locus_tag="PLS34_01650"
     CDS             5282..5605
                     /locus_tag="PLS34_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016712793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86208.1"
                     /translation="MRQALEQLLAGGRDDALLRFSLGGACLKEGDVAAAAEHLARAVA
                     HDPEYSAAWKLLGRALVDAGRPAEAAEAWSRGVEAAERRGDVQAAKEMRVFLKRLDKP
                     GGGAR"
     gene            5605..6993
                     /gene="mgtE"
                     /locus_tag="PLS34_01655"
     CDS             5605..6993
                     /gene="mgtE"
                     /locus_tag="PLS34_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRP86209.1"
                     /translation="MAQNTAAVPGGEMHHEELVAAIREATSAGDAAALRTLIDDEHPA
                     DVAGALEEFEPHHVWAMLDLLPLEDQAGVFGYFERDFQVELAHTAPRAKLARIVTEMH
                     SDERADLFNELSEEQQDALLPALAQAEREDIRRLAGYDEGTAGSIMTSDYALLNETLS
                     AAQAIEKLRHEAPDKETIYRAYVTSPDRRLVGAVRLQDLILATPRTLVRDLMEEEPVF
                     VTVDEDQEEVARKISRYDVLALPVVDAEHRIVGIVTHDDAMDVIQEEATEDFHKVGSV
                     GELDTSVRDASIGLLYRKRIAWLVLLVFGNLLSGAGIHYYEDTIASVVALVFFLPLLI
                     GSSGNAGSQAATLMVRALATGDVQLKDWGKMLGREVLIALLLGLTMALAVSGIGWYRG
                     GPGVAIAVALTMVLVVVVGSLIGMLLPFLLSRFKLDPATASAPLVTTLADASGVFIYF
                     GVATAILFPAGG"
     gene            complement(6998..7993)
                     /locus_tag="PLS34_01660"
     CDS             complement(6998..7993)
                     /locus_tag="PLS34_01660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRP86210.1"
                     /translation="MRIALVSDAWLPQTNGVVRTLRETTRCLEAGGHAVCTVTPTPFM
                     TLPCPTYPEIRLAAWPWRRVRRILDDFRPDAVHIATEGPIGLAGRHWCRDRGLTFTTS
                     FHTRFPEYVRLRAPIPEGWTWRVLRWFHDAAARTLVPTATQQSELAARGFRSPVVWGR
                     GVDTELFHPRRRTGLPGPGPHLVYLGRVAVEKNIGAFLALDVPGTKWVIGGGPALESF
                     REAWPGVNFLGARHGVELASLLASGDVFVFPSRTDTFGIVLLEAMACGMPVAAYPVPG
                     PADVVRHGETGVLDEDLAVAVRGALELDRGACRRQALENTWERATEQFSAHLVPA"
     gene            complement(7990..8790)
                     /locus_tag="PLS34_01665"
     CDS             complement(7990..8790)
                     /locus_tag="PLS34_01665"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRP86211.1"
                     /translation="MSQVKNLRPMTFRTIFISDVHLGSRGCQAGMLLDFLRSVRCETL
                     YLVGDIIDFWSMRRGFYWPQEHNEVVRTVLGMARHGTRVVYVPGNHDELFRDHIGTVF
                     GNVEILARSIHRTADGRRLLVLHGDEFDGVVRCSPLLARIGSRAYDMLIWLNGYLNAC
                     RRYLGYGYWSLAAFLKHRVKNAVQYIGDFEHAVARAARQEGVDGLVCGHIHRPEVRMV
                     DEILYCNDGDWVESCTALAEHRDGRLELLHWAEISAREQQQTLLESAA"
     gene            9095..9568
                     /gene="dtd"
                     /locus_tag="PLS34_01670"
     CDS             9095..9568
                     /gene="dtd"
                     /locus_tag="PLS34_01670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRP86212.1"
                     /translation="MIALLQRVLEASVTVDGEVVGRIGPGLLALAAVERGDDARRAAR
                     LAERILAYRVFEDAEGRMNRSLADTGGGLLLVSQFTLAADTRKGARPSFTPAADPDTG
                     RVLFDALLAAARARHRPVATGRFGARMQVALVNDGPVTFWLRVNPEGGDTGIHSV"
     gene            9621..9863
                     /gene="tatA"
                     /locus_tag="PLS34_01675"
     CDS             9621..9863
                     /gene="tatA"
                     /locus_tag="PLS34_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRP86213.1"
                     /translation="MGLGGISLWQLLIILLIVVLIFGTKRLKSIGSDLGGAVKGFRSS
                     MQDGESEARKEQLADKRKEDADFSESKVEEKDQNRT"
     gene            9869..10231
                     /gene="tatB"
                     /locus_tag="PLS34_01680"
     CDS             9869..10231
                     /gene="tatB"
                     /locus_tag="PLS34_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRP86214.1"
                     /translation="MFDIGFWELALIMLLALLIVGPERLPGLVSTIGNWAGRAKFMAR
                     SLRMQVERELAKEVAEKAEQEGKAPPKTTPAEKSPVQQAAEAAQSAQEEALEAARAKQ
                     LDVQELAESRADERSRQD"
     gene            10209..10982
                     /gene="tatC"
                     /locus_tag="PLS34_01685"
     CDS             10209..10982
                     /gene="tatC"
                     /locus_tag="PLS34_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010672360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRP86215.1"
                     /translation="MSEADRTDNKGEGTLLSHLVELRTRILRMVVAVLLVFVCLVPFA
                     DTIFSLVASPLMARLPEGTSMIATQVASPFLTPFKLALAAAVFLAMPILLYQAWAFVA
                     PGLYKHERRLVLPLMASSIVLFYGGVAFAYYIVFPLMFAFFTTVAPSGVTVMTDIAAY
                     LDFVLALFFAFGFAFEVPIATVLAVWAGFTTPEKLAQKRPYVLLGAFVMGMLLTPPDV
                     ISQTLLAVPVYILFEAGIVMARVMVPGHKEVEAQQREGS"
     gene            complement(10989..12002)
                     /locus_tag="PLS34_01690"
     CDS             complement(10989..12002)
                     /locus_tag="PLS34_01690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRP86216.1"
                     /translation="MSPELALRALLGLLPVIVFLAALLWLDSYKLVPLRSVLATMAAG
                     TLIAGACYGVNLALLGALPMDFGSYSRFVAPIVEESLKGLVLVYLVRRHAIGFPTDAA
                     IFGFAVGTGFAVVENLYYLAMFADSHIAVWVVRGFGTAIMHGGATAIFGIVAHTLAEQ
                     RQSHGVTEFLPGLLGATLVHSLYNQFLFAPVYSTLGIILLLPPIIYFVFQRSERTLQR
                     WLAVDFDADTQLLELINSGAVEDSRLGRHLESVRQHFSGPIVADMLCYLRLHVELSMR
                     ASATLLMRESGIEPEVDPEVRAMVEEMRFLEKSIGKTGQHAMKPFLRLSSRDLWQLYL
                     LGK"
     gene            complement(12045..14996)
                     /locus_tag="PLS34_01695"
     CDS             complement(12045..14996)
                     /locus_tag="PLS34_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86217.1"
                     /translation="MRAANVAIIIAVLSCGVADPAGAQALSLLETENQRLLYIDPATT
                     YLAPYAAQSFENSLLGQRRIFGYEPAEKVTVLLMDFRDSGNAGAVPLPRNAVLVDVAP
                     KPFTFETSESAERMYSWMNHELVHLVSTEMTSADDRRWRKLFGGKVAATADHPETILY
                     EYLTNPRLASPLWYHEGIAVFAQTWMAGGFGRAQGAYDEMVFRSMVRDGSRFYDPLGL
                     VAEGVKSDFQVGVNAYLYGTRFMSYLAYVHSPQQVVDWVARREGSRRHYSSEFRRVFG
                     MSLEQAWADWVRWEHEFQGANLESVRQVPPTRYRNLSSRALGSVSRAFVDEATGTMYA
                     GVRYPGIVAHIAAISLVDGSVRRLVDIKGPMLFRVTSLAWDPAARVLYYTTDNGAFRD
                     VMALEVDSGRTRMLLEDARIGELVLNPSDRALWGVRHLNGIATLVRIPHPYTEWHQVH
                     SLPYGVTMYDLDISPDGRLLAASFGEINGDQSVRLMQIEAVLAGDMAPVASFDFGLSV
                     PEGFVFSPDGSFLYGSSYYTGVSNIFRYQLASGEIEAVSNAETGFFRPLPRADGSLVV
                     FQYSGEGFMPALITPEPLDSLGTIDFFGRRIIERHPVLEEWRVGSPADIPLRDMTIAE
                     GHYDAFSSMGLESIYPVLEGYKSSIGAGLHAAISDPVGFHRLGLTGSITPNQPGSEQG
                     HVRIRHDYQRIDQLLPGSWTTELTWNRADFYDIFGPTRLSRKGYSGEVTYRRPLIYDK
                     PRLMDLTVRAGYWGNLEELPYAQDVGTTFDALAQASARLDYEYLHRSLGAVDDEKGFK
                     WRAIAGANHANSSATPFSHAGFDVGWALPGGHSSIWLRSAAGFAVGDRGEEFSNFYFG
                     GFRNNYVDHQEVKRYREPFSLPGFEIDEIRGRTFARTMLEWNLPPARFRRVGTPDFHL
                     SWARPAIFAAALVTDPDVDSRQWYSAGFQVDFQFTLLSRKEMTFSIGYAAAFSDDGHE
                     GNEVMASLKLF"
     gene            complement(15129..16490)
                     /locus_tag="PLS34_01700"
     CDS             complement(15129..16490)
                     /locus_tag="PLS34_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86218.1"
                     /translation="MSEINRSNTGRSKTVGIVAGLAVAVAVVVFITSNQPPAGTETAG
                     TIAPATRYRAEPIAAADVNLGDQALQEFLQTDVYDRLIKDEAFQNAMQSEAFRNALQD
                     AALRNAMQSTALQNAMQDVAFRNAIQDVAFQNAMQSTAFQNALQSTAFQNAMQSTAFQ
                     NAMQSTAFQNALQDTAFQNALQSTAFQNAIQDTAFQNAMQDTAFQNALQSTAFQNAMQ
                     DTAFQNAMQNTALQNALQNAAFLNALASQSLQDAMQSDAFQAAMRNDAALQNALQSDA
                     MQNALQSTAFQNALQDVAFQNALQSTAFQNAMQSTAFQNAMQDVAFRNALQSTAFQNA
                     LQSTAFQNAMQSTAFQNAMQDVAFQNALQNTAFQNALQSTAFQNALQSTAFRDALQST
                     AFQNALQSTAFQDALQNTAFQNAMQSTAFQNALQNNAFLNALQSQAFQNAMANAMADV
                     MRN"
     gene            complement(16578..17333)
                     /locus_tag="PLS34_01705"
     CDS             complement(16578..17333)
                     /locus_tag="PLS34_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86219.1"
                     /translation="MDQRILELIHGDVDGELTPSEQEELQHWLAVSETARQEHARARS
                     LHDLLAAVQAQDPPSGMRATILDALPPRATPTDDFATGRRRSGLGLVAALAATAAGVV
                     FLLQRDPDVPQLDPASLAGTISRPAAGPDAPVWRLEDAAVSGSLRLRQAEHGLYMEVD
                     VEAEGPITLVARSDGRPLEVEGLVPIDTPPASPKLADGGIHMLHSGKHRYAILLRRPA
                     GADEPVELAVYAGDRLVGETRLDIGRAGAAPQD"
     gene            complement(17333..>17692)
                     /locus_tag="PLS34_01710"
     CDS             complement(17333..>17692)
                     /locus_tag="PLS34_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP86220.1"
                     /translation="DFGHRFYSWIYRIAINESINFLHSRQRYAPLDDVAECVDENDGP
                     PEAYGNAELHRRVQAAMMTLSPEHRAVITLRHFTECSYQDIANVLGIPEKTVKSRLFD
                     ARRLLRGQLADDGTGAP"
BASE COUNT         2891 a         5825 c         6031 g         2945 t
ORIGIN      
        1 cggcccggtc ccgcggacaa ccgccaaccg aagcgcaccc atggcggcgc tgccccgtcg
       61 gcggccgtaa accggtgtct gaccccggtt ttgcgcccgc aggcgcccgg cgtggctagt
      121 acaatggcgc gatggacatc acccccatcc tcgacccgct gaacgacgca cagcgcgagg
      181 ccgtcacggc gccgtccgac gcgccggtgc tggtgctggc gggcgcgggc agcggcaaga
      241 cccgcgtgct ggtccaccgc atcgcctggc tggttcaggt cgagggcgtg tcgccgtggg
      301 gcgtgctggc ggtgacgttt accaacaagg ccgccggcga gatgcgcagc cgcatcgagg
      361 agctgctcgg catgccggcc ggcaacctct gggtcggcac tttccacggg ctggcgcacc
      421 gcctgctgcg gctgcactgg agggaagcga agctgccgca gaccttcacg gtgctcgact
      481 cggaggacca gctgcggctg atccggcggc tcctcaaggc gctggagctg gacgagaacc
      541 gctgggtgcc gcgcgaggtg cagtggttca tcaatgcgcg caaggacgag ggcctgcgcg
      601 ccgcccacct ggacgccggc gacgacccga accgccgcat gttcatccgt atctacgagg
      661 cctaccaggc acagtgcgag cagagcgggc tggtggattt tgccgagctg ctgttgcgcg
      721 cgttcgaact gtggcgcgag aaccccgaac tgctggacca ttaccggcgg cgcttccggc
      781 acctgctggt ggacgagttc caggacacca acgccatcca gtacgcctgg ctgcgcctgc
      841 tggcgggacc ggagggcaag ccgttcatcg tcggcgacga cgaccagagc atctacggct
      901 ggcgcggtgc ccgggtcgag cacatccgac gcttccgcaa ggactttccc ggcacgcggc
      961 tggtgcgcct ggagcaaaat taccgctcta ccggcaacat cctcaacgcg gccaacgcgc
     1021 tgatcgcgaa caaccgcgag cgcctcggca agaagctctg gaccgaggga cacgccggcg
     1081 aaccgatccg cctgtatgcg gcgtacaacg agcgcgacga ggccgagttc gtggtgggac
     1141 gcatccgcgc cgccatcgag cagggcgcgg ccaacagcga catggccgtg ctgtatcgct
     1201 ccaatgcaca gtcgcgcgtg tttgaagaag cgctcatcca ggcccgcatt ccctatcgcg
     1261 tctacggcgg cctgcgcttc ttcgagcgcg ccgagatcaa ggatgcgctg gcctacctgc
     1321 ggctgatcgc gaaccgcgac gacgattccg ccttcgagcg agtcgtgaac ctgcccacgc
     1381 gcggcatcgg cgcccgcacc ctcgaggcat tgagggacta cgcccgtgcc aacgccacct
     1441 ccatgtggcg tgccgccggc gcgctggtca cggacgggct gagcgcccgt gcgggccagg
     1501 ccctgcacgg ctttctcgcg ctgatcgagg cgctcgatcg cgacacgcgc gcgctggagc
     1561 tgcacgagca ggtggaccac gtcatccagg ccagcgggct gatcgcgcac cacggcaagg
     1621 agaagggcga gcgcggcgag gcgcggatcg agaacctcga ggagctggtc agcgcggcgc
     1681 gtggcttcga gccggagccg gactccgaga tgcgcctgct ggacgagttc ctggcgcatg
     1741 ccgcgctgga ggcgggcgaa gggcagggcg gcgcctggga ggactgcgtg cagatgatga
     1801 cgctgcactc cgccaagggc ctggagtttc ccatcgtgtt cctggcgggc atggaggacg
     1861 ggctgttccc gcacaagcgc agcctgctgg acgccgatgg cctcgaggaa gagcggcgac
     1921 tcgcctatgt cggcacgact cgggccatgc gcgagctgta tctcagctat gcagagcagc
     1981 gccgcctgca cggcgtggac aattacggga ttccgtcgcg cttcatcggc gagctgccgg
     2041 ccgagctggt cgaggaaatc cggccggcgg tgcgcgtgtc gcggccggcc gcggcgccga
     2101 gcggccgatt ccgcgaccag gcaccgggtg gcatttccct gggccagcgc gtacgccacg
     2161 gccagttcgg cgagggcgtc gtgctgaacc acgagggcca cggcagccac gcgcgggtgc
     2221 aggtgaactt cgagcaggcc ggcccgaagt ggctggtgct cgcctatgcg cgccttgaag
     2281 tgatgtaaca tcggcagcgc cgcccaggcc ggaaacaagc aacatgaaga tcctcatcct
     2341 cggcgcgggc acggtcggat ccaccgccgc agaatcgctt tccgaggaaa acgagatcac
     2401 cgtggtggac ctcaaggggc agcacctgcg tgaactgcag gaccgtctcg acatccggac
     2461 cgtggagggc cacgcctccc atccgcgcgt gctggagcag gcgggcgccg ccgacgccga
     2521 cattctcgtc gccctcaccg acagcgacga aaccaacatg atcgcgtgcc agatcgccta
     2581 cagcctgtac cggacgccgg tacgcatcgc gcgcatccgt gccaccgagt acacctcgcg
     2641 caaggagttg ttcaccaaca agaccatccc ggtcgacacc atcatcagcc ccgagcagct
     2701 ggtcaccgag cacatcgaac agctgatcca ttatcccggt gcgcaccagg tgctggattt
     2761 tgccgaggga cgggttcggc tggtcggcct catggcaaaa gccggcgggc cgctcatcgg
     2821 ccaggagctc cgcgagctgc ggcggcacat tccgcacacc gacgcgcggg ttgccgccat
     2881 ctaccgccgc agcaccatcc cgcccgtggg caacgaggag ccgctctacg agaccatcct
     2941 gccggagggt gatacggtca tccggccgaa cgacctggtg tttttccttg ccgcccgcaa
     3001 ggatatccgc gtcatgatga gcgagctgct gaaactcgag cacccggcgc gcagcatcat
     3061 catcgcgggc ggcggccaca tcggcttcga gctggcgcgg cggctcgaga acaccaatcg
     3121 ggtcaaggtg atcgagcgcg accacgcgcg cgcgcgctac atcgccgagc gcctgcgcca
     3181 cgccgtggta ctcaccggag acgcggtcga cgaggagctg ctggcggaag agaacatcga
     3241 caaggtcgac gtgttctgcg cgctcaccag cgcggaagag gccaacatcc tgtcgtcgtt
     3301 cctggccaag cgcatgggcg cgtcgcgggt catcgccctg atcaaccgcc cgtcctacgc
     3361 gaaactggtc gaggaccagt acgtcgacat cgcggtgtcg ccgcaggaga tcacgctgag
     3421 cgccctgctc gccaaggtgc ggggcatgcg gctggacgtc ggccaggtgc atgcgctgcg
     3481 cagcggcacg gcggaagcca tcgaggccgt cgcccacggc gactcccgca catccaaggt
     3541 cgtcggcaag gcgatcgagg acgtgccgtt gccgccgggc gccaccatcg gcgccatcgt
     3601 gcgcgacgag gaggtgctga tggcgcacca cgacaccgag attcgcagcg aggaccacgt
     3661 cattctcttc ctcaccgacc gcagccagat tcccgcggtc gagaagctgt tccaggtcgg
     3721 cgtgacctac acctgatggc gagccgcgcg cggtgaacgg tctcgtcgta ctgcggatca
     3781 tcgggctgct gttgtcgctg ttcagcctga cgatgctgcc gccggtggtc gtctccttac
     3841 tgtataacga cggcgcctgg ccggcgttcg tcgaggcgtt tttcatcttc ctcgcagccg
     3901 gactggcatt gttcctgccg ctgcgccggc gtcgcgccga gctgcgcatc cgcgacggct
     3961 tcctggtcgt ggccggtttc tgggtggtgc tgggcttcgc cggcgccgtg ccgctggtgc
     4021 tggtcgagat cccgatcatg agcatcacgg acgcggtgtt cgaggccgta tccggcttca
     4081 ccaccaccgg ggccacggtg ctggtcggcc tcgataccct gccgcactcg gtactgtggt
     4141 accggcagca gagccactgg atcggcggca tgggcatcat cgtgctggcg gtggcgatcc
     4201 tgccggtgct gggcgtgggc gggatgcagc tctaccgtgc cgagacgccg gggccgatga
     4261 aggacaccaa gctgacgcca cgcatcaccg agacggccaa ggccttgtgg atcacttacg
     4321 tagggctcac cgcgatctgc gcggtgtcct accggctggc gggcatgaac tggttcgatg
     4381 ccatcggcca cgccttcagt tcgatcgcca ccgccggttt ttcgacacac gacgccagca
     4441 tcgcctattt cgacagcggc ctgatcgagt ggctgacggc gctgttcatg ttcctcggcg
     4501 ccacgagttt cgcgctgcac ttctacgcaa tgcgccggct gatgcccgac attcacctga
     4561 ccacgcgcgg cggagtgccc aggttttcct ggccgcatcg gcgcaaggtg tccctgaaag
     4621 catccatcgg cggctatttc aacgatccgg aattccgtgc gttcatggcc atccaggccg
     4681 gactgatcgc gatcatcgcc gcttacctga ccctcaaggg gcaccatgcc cccggagaat
     4741 cgctcaccaa ggccgtgttc cagtcggtct cgttcggcac cacgacgggc tacacgtcga
     4801 cggattactc cgcctggccg ggggcgctgc cgacgctgct gatcctggcg agcttcatcg
     4861 gcggctgcgc cggctccacg gccggcggca tgaaagtgtt gcgctggctg ctgattttca
     4921 agcagggctg gcgcgagctg gcccgcctgc tgcaccccaa tgcggtgatg atggtgcgcc
     4981 tcggtgagcg cggcgtgccc agccgggtga cggaggccat ctggggcttc ttctcggtct
     5041 acgtggtggt gttctgtatc ctgatggcag tgatgatgac catcggactc gaccaggtca
     5101 ccgccttttc ggcggtggcc gcgacgctga acaacctcgg cccgggcctc ggcgaggtca
     5161 gcatcacgtt cggcaccgtg ccggatacgg ccaaatgggt gggggtgttg ggcatgatcc
     5221 tcggccgcct cgagattttc acgctgctcg tgctgctgac cccggaattc tggcggcgtt
     5281 gatgcgacag gcgctcgaac agctgctggc cggggggcgg gacgacgccc tgctgagatt
     5341 ctcgctgggc ggcgcgtgcc tgaaggaggg ggacgtggcc gcggcggccg agcacctggc
     5401 gcgcgccgtt gcacacgacc cggagtactc ggcggcctgg aagctgctgg gccgggcgct
     5461 ggtcgacgcc gggcggccgg cggaggccgc ggaggcctgg tcgcgcggcg tcgaggcagc
     5521 cgagcgacgg ggtgatgtac aggctgcgaa ggaaatgcga gtgttcctga agcgcttgga
     5581 caagccgggt ggaggggcac gctgatggcg caaaacacgg cagccgtccc cggtggggaa
     5641 atgcaccacg aggagctcgt cgcggcgatc cgcgaggcca ccagcgccgg tgacgccgcc
     5701 gcgctgcgca cgctgatcga cgacgagcat ccggcggacg tggcgggcgc gctcgaggag
     5761 ttcgagccgc accacgtctg ggcgatgctc gacctgttgc cgctcgagga ccaggccggc
     5821 gtgttcggct atttcgagcg ggacttccag gtcgagctgg cgcacaccgc gccgcgcgcc
     5881 aagctggcca ggatcgtcac ggaaatgcac tcggacgagc gggccgacct gttcaacgag
     5941 ctgtccgagg agcagcagga cgcgctgttg ccggccctgg cgcaggcgga gcgggaggac
     6001 atccgtcgtc tcgccggcta cgacgagggg accgccgggt cgatcatgac ctcggactac
     6061 gccctgctca acgagaccct ctcggcggcg caggccatcg agaagctgcg tcacgaggcg
     6121 ccggacaagg aaacgatcta ccgcgcctat gtcaccagcc cggaccgccg gctggtcggt
     6181 gccgtccgcc tgcaggacct catcctcgcc acccccagga cgctggtgcg ggacctgatg
     6241 gaagaggagc cggtgttcgt caccgtggac gaggaccagg aagaggtggc ccgcaagatc
     6301 tcgcgttacg acgtgctggc gctgccggtg gtggacgcgg agcaccgcat cgtcggtatc
     6361 gtcacgcacg acgacgccat ggacgtcatc caggaagagg ccaccgagga cttccacaag
     6421 gtcggctcgg tcggcgagct ggacaccagc gtacgcgacg cctccatcgg cctgctgtac
     6481 cgcaagcgca tcgcgtggct ggtgctgctg gtgttcggca acctgctgtc gggcgccggc
     6541 atccactatt acgaggacac catcgcctcg gtggtggcgc tggttttttt cctgccgctg
     6601 ctgatcggct cgagcggtaa cgccggctcg caggccgcca cgctgatggt gcgcgcgctg
     6661 gccaccggcg acgtgcagct caaggactgg ggcaaaatgc tcgggcgcga ggtgctgatc
     6721 gcgctgctgc tcggactgac catggcgctg gcggtttccg gcatcggctg gtaccgcgga
     6781 ggtcccggcg ttgctatcgc cgtcgcgctg acgatggtgc tggtggtggt ggtgggcagc
     6841 ctcatcggca tgctgctgcc gttcctgctc agccgcttca agctggaccc ggccacggcc
     6901 agcgcgccgc tggtgacgac gctggccgac gcatcgggcg tgttcattta tttcggcgta
     6961 gcgacggcga tcctctttcc cgccggcggc tgagcgctca ggccggcacc aggtgggcgc
     7021 tgaactgctc ggtggcccgc tcccaggtgt tctccagcgc ctggcggcgg catgcgccgc
     7081 gatcgagctc gagcgccccg cgcaccgcca cggcgagatc ttcgtcgagc accccggtct
     7141 cgccgtgccg gacgacgtcg gctggtccgg gcacgggata ggcggcaacc ggcatgccgc
     7201 aggccatggc ttccagcagc acgatgccga aggtatcggt gcggctcgga aagacgaaca
     7261 cgtctcccga cgccagcaac gaggccagtt cgacgccgtg gcgggcgccg aggaagttca
     7321 ccccgggcca ggcctcgcgg aacgattcca gcgccgggcc gccaccgatg acccacttgg
     7381 tgcccggcac gtccagcgcc aggaaggcgc cgatgttctt ctccaccgcg acgcggccca
     7441 ggtacaccag gtgcggtccg gggcccggca ggccggtgcg gcgccgcgga tggaacagct
     7501 cggtgtcgac gccgcgcccc cacaccaccg ggctgcggaa gccgcgtgcc gcgagttcag
     7561 actgctgggt agccgtgggc accagcgtgc gggcggctgc gtcgtggaac cagcgcaaca
     7621 cgcgccaggt ccagccttcg ggaatcgggg cgcgcaggcg gacgtactcg gggaagcgcg
     7681 tgtggaagga ggtggtgaaa gtcaggccgc gatcccggca ccagtgacgg ccggccaggc
     7741 cgattgggcc ctcggtggcg atgtgcaccg catcgggccg gaaatcgtcg aggatgcgcc
     7801 ggacccggcg ccagggccag gcggccaggc ggatctcggg gtaggtcggg cagggtagcg
     7861 tcatgaacgg cgtcggcgtc acggtgcaga ccgcgtggcc gccggcctcc aggcagcgcg
     7921 tggtttcccg caacgtgcgc actacgccgt tggtctgcgg cagccaggcg tccgacacga
     7981 gtgcgatcct catgcggcgc tctccagcag ggtctgctgc tgctcgcgtg cggagatctc
     8041 tgcccagtgc agcagctcga gtcgcccgtc gcgatgctcg gccagtgccg tgcaactctc
     8101 cacccagtcg ccgtcgttgc agtacagaat ctcatcgacc atgcgcacct ccgggcggtg
     8161 gatgtggccg cagaccagtc cgtccacgcc ctcctgccgc gcggcgcggg ccactgcgtg
     8221 ctcgaagtcg ccgatgtact gcaccgcgtt cttcacgcgg tgcttgagga acgcggccag
     8281 cgaccagtac ccgtagccga gatagcgccg gcacgcattc aggtagccgt tcagccagat
     8341 gagcatgtcg taggcgcggc taccgatccg cgccagcaac gggctgcagc gcaccacgcc
     8401 gtcgaactcg tcaccgtgca gcaccagcag ccgccggccg tcagccgttc gatggatgct
     8461 gcgggcgagg atttccacgt tgccgaagac ggtgccgatg tggtcgcgaa acagctcgtc
     8521 gtgattgccg gggacgtaca caacgcgggt gccgtgccgc gccatgccga gcacggtgcg
     8581 cacgacctcg ttgtgttcct ggggccagta aaagccgcgg cgcatgctcc agaaatcgat
     8641 gatgtcgccg accaggtaga gcgtttcaca gcgcaccgag cgcaggaagt cgagcagcat
     8701 ccccgcctgg cagccgcgtg agccgagatg cacgtcggag atgaaaatgg tccggaaggt
     8761 catcggccgc aggttcttca cttgggacat gtatggcctc cgccgcttgc ccgggggcac
     8821 aatggccgcg ggtggttaca ggcgcatcac cgcgtggtga cacgggtatt gcagtgcaat
     8881 gcagagtcgg gtggggccat ccacccgggc cggccgcacc gggcgggcgg gaagcgtgtt
     8941 gctaattccc actttggcaa acaaattatg ctcgtgggaa acatggctac ttgttaacga
     9001 tcttcttcgc gattggtggg ataatgatgt caacaaaccc gggggctcgc gtggtctggg
     9061 ccggaaattg gggtctgacc ccggttcttg gcggatgatc gcgctgctcc agcgggtcct
     9121 cgaggcctcc gtcacggtgg acggcgaagt ggtcgggcgg atcggcccgg ggctgctggc
     9181 gctggccgcc gtggagcgcg gcgacgacgc ccggcgggcg gcccgcctgg cggagcggat
     9241 cctggcttac cgggtgttcg aggacgccga gggccggatg aaccgctcgc tggcggacac
     9301 cggcggcggg ctgctgctcg tttcccagtt caccctcgcg gcggacaccc gcaagggggc
     9361 aaggccgagc ttcacgccgg cggcggaccc ggacaccggc cgggtcctgt tcgacgccct
     9421 gctggccgcc gcgagggctc gccaccggcc ggtggcaacg ggccgcttcg gggcacgaat
     9481 gcaggttgcg ctggtcaatg acggccccgt gactttctgg ttaagggtga acccggaggg
     9541 cggcgacacg ggcatccata gcgtatgatt gcaggtcttc gcggcgtcac ggccgtgggc
     9601 cgtctaattc ggaggatcgc atgggtctcg gtggcatcag tctttggcaa ctcctgatca
     9661 tcctgctgat cgtggtgttg attttcggta ccaagcgcct gaagagcatc ggctccgatc
     9721 tcggcggcgc cgtcaagggc ttccgcagct cgatgcagga cggtgagtcg gaggcgcgca
     9781 aggagcagct cgcggacaag cgcaaggagg acgccgactt ctccgagagc aaggtcgagg
     9841 agaaggacca gaacaggacc tgacgccggt gttcgacatc ggtttctggg agcttgcgct
     9901 gatcatgctg ctggccctgc tcatcgtggg gccggagcgg ctgcccggct tggtgagcac
     9961 gatcggcaac tgggccggac gggcgaagtt catggcgcgc agcctgcgca tgcaggtcga
    10021 gcgcgagctg gccaaggaag tggccgagaa agccgagcag gaaggcaagg cgccgccgaa
    10081 gacgacgccg gcggaaaagt ccccggtgca gcaggccgcc gaggcggcac agagtgcgca
    10141 ggaagaggcc ctggaagcgg cgcgtgcgaa gcagctggac gtgcaggagc tggcggagag
    10201 ccgggccgat gagcgaagcc gacaggactg acaacaaggg cgaaggcacg ctgctgtccc
    10261 atctggtcga gctccgcacc cggatcctga ggatggtcgt ggcagtgctg ctcgtgttcg
    10321 tctgcctggt gccgttcgcc gacaccatct tctcgctggt ggccagcccc ctgatggccc
    10381 gcctgcccga ggggaccagc atgatcgcca cgcaggtggc gtcgcccttc ctgacgccgt
    10441 tcaagctggc gctggccgcc gcggtgttcc tggccatgcc gatcctgctc taccaggcct
    10501 gggccttcgt cgcacccggc ctctacaagc acgagcgccg cctggtgctg ccgctgatgg
    10561 cctccagcat cgtgttgttc tacggtgggg tagcctttgc gtactacatc gtgtttccgc
    10621 tgatgttcgc cttcttcacc accgtggcgc cctccggcgt gaccgtgatg accgacatcg
    10681 cggcctacct ggacttcgtg ctggcgctgt tttttgcctt cggcttcgcc tttgaagtac
    10741 cgatcgccac cgtgctggcg gtctgggccg gcttcacgac gccggaaaag ctggcgcaga
    10801 agcgtcccta cgtgctgctc ggggcgttcg tgatgggcat gctgctgacg ccgccggacg
    10861 tcatctcgca gaccctgctg gccgtgccgg tctacatcct gtttgaggcc ggcatcgtca
    10921 tggcccgcgt catggtcccc ggccacaagg aagtcgaggc gcaacagcgc gaggggtcgt
    10981 gagcccgctt acttgcccag caggtagagc tgccaaaggt cacgcgaact gagccgcagg
    11041 aagggcttca tggcgtgctg gccggtcttg ccgatgctct tctccaggaa gcgcatctcc
    11101 tccaccatgg cgcgtacttc cgggtcgact tccggctcga tgccgctctc gcgcatcagc
    11161 agcgtcgcgc tcgcgcgcat ggacagctcg acgtgcagcc gcaggtagca caacatatcg
    11221 gccactattg gtccggaaaa atgctggcgc acactctcca ggtgccggcc gaggcgtgaa
    11281 tcctccactg cgcccgagtt gatcaattcg agcagctgcg tatccgcgtc gaaatcgacc
    11341 gccagccagc gctgcagggt gcgctcactg cgctggaaca cgaaatagat gatcggcggc
    11401 agcagcagga tgatgcccag cgtcgagtac accggcgcga acaggaactg gttgtacagc
    11461 gagtgcacca gcgttgcgcc cagcagcccg ggcaggaact ccgtgacgcc gtgcgactgc
    11521 cgttgctcgg ccagcgtatg cgccacgatg ccgaagatcg ccgtggcgcc gccgtgcatg
    11581 atggccgtgc cgaagccgcg cacgacccac accgcgatgt gcgaatccgc gaacatcgcg
    11641 aggtaataga ggttctcgac gaccgcgaag cctgtgccga ccgcgaaacc gaagatcgcc
    11701 gcgtcggtgg gaaagccgat ggcatgcctg cgcaccaggt agaccagcac cagccccttg
    11761 agcgactcct cgacgatcgg cgccacgaaa cgggagtaac tgccgaaatc catcggcagc
    11821 gcgcccagca gggcgagatt gacgccgtag caggcgccgg cgatgagtgt tccggccgcc
    11881 atcgtcgcca gcaccgatct cagcgggacc agcttgtagc tgtcgagcca cagcaacgcc
    11941 gccaggaaaa cgatcaccgg cagcaatccc agcagggccc gcaacgcgag ttccggcgac
    12001 atcagcagat cttctccccg accgcctggc cgatgctcag gctctcagaa cagtttcagc
    12061 gacgccatga cctcgttgcc ctcgtggccg tcgtcgctga acgcggccgc gtagccgatc
    12121 gaaaacgtca tctccttgcg tgacaggagg gtgaactgga agtccacctg gaaacccgcc
    12181 gagtaccatt gcctgctgtc tacatccgga tcggtcacca gcgccgcggc gaagatggcc
    12241 ggccgcgccc aggagagatg gaagtcgggc gtgcccaccc ggcggaagcg ggccggcggc
    12301 aggttccact ccagcatggt gcgcgcgaag gtgcgcccgc ggatctcgtc gatctcgaag
    12361 ccgggcagtg agaacggctc gcggtagcgc ttcacctcct ggtggtccac gtagttgttg
    12421 cggaacccgc cgaagtagaa gttcgagaac tcctcgccgc ggtcgccgac ggcgaagccg
    12481 gccgcacttc gcaaccagat ggacgagtgc ccgccaggca gcgcccagcc gacatcgaaa
    12541 ccggcgtgag agaagggcgt ggcgctggag ttggcgtgat tggcgccggc gatcgcccgc
    12601 cacttgaaac ccttctcgtc gtccaccgca ccgagcgagc gatgcaggta ctcgtagtcg
    12661 agccgcgcgc tcgcttgcgc cagcgcgtcg aaagtcgtgc cgacgtcctg cgcgtagggc
    12721 agctcctcga gattgcccca gtagccggcc cgcaccgtca ggtccatcag ccgcggcttg
    12781 tcgtagatca gcgggcgacg atacgtcact tcgcccgagt aacccttccg actcagccgg
    12841 gtcgggccga agatgtcgta gaaatcagcc cggttccacg tcagttcagt ggtccagctg
    12901 ccgggcagca gctggtcgat tctctggtaa tcgtggcgga tgcgcacgtg tccctgctcg
    12961 ctgcccggct ggttcggcgt gatcgagccc gtcagcccca gccggtggaa gccgacgggg
    13021 tcactgatgg cggcgtgcag gccggcgccg atcgagctct tgtagccttc caggaccgga
    13081 tagatcgact cgaggcccat gctggaaaat gcgtcgtagt gtccctcggc gatggtcatg
    13141 tcacgcaggg ggatgtcggc cggtgagccg acgcgccatt cctccagcac cgggtgccgc
    13201 tcgatgatcc gccgaccgaa aaagtcgatg gtcccgaggc tgtcgagcgg ctccggcgtg
    13261 atcagcgccg gcatgaagcc ttcgccgctg tactgaaaca ccaccagcga cccgtccgcc
    13321 cgcggcaggg gccggaaaaa tcccgtctct gcgttgctca ccgcctcgat ctcgccggag
    13381 gccagctggt agcggaagat gttggacacg ccggtgtaat agctgctgcc gtacaggaag
    13441 ctgccgtcgg gtgagaacac gaaaccctcg ggcaccgaca gcccgaaatc gaagcttgcg
    13501 accggcgcca tgtcgcccgc cagcacggcc tcgatctgca tcaggcggac cgactggtcg
    13561 ccgttgatct ccccgaagga ggcagccagc agccggccgt cgggcgaaat gtcgaggtcg
    13621 tacatggtta cgccataagg aaggctgtgc acctggtgcc actccgtgta aggatgcgga
    13681 atcctgacca gggtggcgat gccgttgaga tggcgaacgc cccacagtgc acggtccgaa
    13741 gggttgagca ccagctcccc gattcgcgcg tcctccagca gcatgcgggt cctgccgctg
    13801 tcgacctcca gcgccatgac atcgcgaaag gcgccattgt ccgtcgtgta gtaaaggacc
    13861 cttgcggccg gatcccaggc cagcgaggtc acccggaaca acatcggccc cttgatgtcg
    13921 accagccgcc tgaccgaccc gtccaccagc gagatggccg cgatgtgcgc aacgatgccg
    13981 ggatagcgga cgcccgcgta catcgtgccc gtggcctcgt ccacgaaggc ccgcgacacg
    14041 gaacccagcg cacgggagga gaggttgcga tacctcgtgg gcggaacctg ccggacggac
    14101 tcgagattgg cgccctggaa ctcgtgctcc cagcggaccc agtccgccca ggcctgctcg
    14161 agcgacatgc cgaacacccg gcggaactcg ctggagtaat gccgccggct gccctcgcgc
    14221 cgcgcgaccc agtcgaccac ctgttgcggc gaatgcacgt aggccaggta gctcatgaac
    14281 cgagtgccgt agaggtacgc gttgacgccg acctggaagt ccgacttcac gccttccgcc
    14341 accaggccga gcggatcata gaagcgcgag ccgtcccgta ccatggagcg gaacaccatt
    14401 tcgtcgtaag cgccctgggc gcgcccgaag ccgccggcca tccaggtctg ggcgaacacg
    14461 gcgatgccct cgtgatacca gagcgggctc gccaggcgcg ggttggtcag gtactcgtac
    14521 aggatggtct ctgggtgatc ggcggttgcc gcgaccttgc cgccgaacag cttccgccag
    14581 cgccgatcgt ccgcgctggt catctcggtg ctcaccaggt gcaccagctc gtggttcatc
    14641 cacgagtaca tgcgctcggc cgactccgag gtctcgaagg tgaacggctt gggcgccacg
    14701 tccaccagca cggcgttgcg cggcagcggc accgcgccgg cattgcccga atcgcgaaaa
    14761 tccatcaaca ggacggtcac cttctcggcc ggttcgtagc caaagatgcg ccgctgtccg
    14821 agcagcgaat tctcgaagct ctgcgcagca tagggtgcca ggtaggtggt ggccggatcg
    14881 atgtagagca gccgctggtt ctcggtctcc agcaggctca gcgcctgcgc gccggccggg
    14941 tccgccacgc cacacgacag cacggcgatg atgattgcga catttgctgc gcgcattcgg
    15001 ctccgcactt cttattcgtg tgctgcggca aaaaaagagg atacaacgaa aagtctccgt
    15061 tgcatcctct gtttttggcg gtgcgtcggc gccatgccgt gacccgcccg cgtcggcact
    15121 cgccggcgct agttgcgcat cacgtcagcc atggcgttcg ccatcgcgtt ctggaaagcc
    15181 tggctctgca gcgcgttgag gaaggcattg ttctgcagcg cattctggaa ggcggtgctc
    15241 tgcatggcgt tctggaaggc cgtgttttgc agtgcgtcct ggaaggcggt gctctgcagc
    15301 gcattctgga aggccgtgct ctgcagcgca tcccggaaag cggtgctctg caacgcgttc
    15361 tggaaggcgg tgctctgcag cgcattctgg aaggccgtgt tttgcagcgc gttttggaac
    15421 gcgacatcct gcatggcgtt ctggaaggcc gtgctctgca tggcattctg gaaggcggtg
    15481 ctctgcagcg cattctggaa ggccgtactc tgcagcgcgt tccggaacgc gacatcctgc
    15541 atggcattct ggaaagccgt gctctgcatg gcattctgga aggcggtgct ctgcagtgca
    15601 ttctggaaag cgacgtcctg cagggcgttc tggaaggccg tgctctgcaa ggcgttctgc
    15661 attgcgtcac tctgcagcgc attctgcagc gccgcatcgt tccgcatggc cgcctggaaa
    15721 gcatcgcttt gcatcgcgtc ctgcagggac tggctggcca gggcattcag gaaggcggcg
    15781 ttctgcagcg cgttttgcag cgcagtgttc tgcattgcgt tctggaaggc cgtgtcctgc
    15841 atggcgttct ggaaggccgt gctctgcagc gcgttctgga aggccgtgtc ctgcatggcg
    15901 ttctggaagg ccgtgtcctg gatcgcgttc tggaaggccg tgctctgcaa ggcgttctgg
    15961 aaggccgtgt cctgcagcgc gttctggaag gcggtgctct gcatggcgtt ctggaaggcc
    16021 gtgctctgca tggcgttctg gaaggccgtg ctctgcagtg cattctggaa agcggtgctc
    16081 tgcatggcgt tctggaacgc gacgtcctgg atggcgttcc ggaaggcgac gtcctgcatg
    16141 gcgttctgca gggccgtgct ctgcatggcg ttgcgcagcg cggcgtcctg cagcgcattc
    16201 ctgaacgcct cgctctgcat cgcgttctgg aaggcctcgt ccttgatcag ccggtcatag
    16261 acgtcggtct ggagaaattc ctgcaaggcc tggtcgccca ggttgacgtc tgccgcggcg
    16321 atcggttcag cccgatagcg ggtcgccggg gcaatggtcc cagcggtctc tgtgcccgcg
    16381 ggtggctggt tgctggtgat gaacacgacg actgcgacag cgacagccag gccggcgacg
    16441 atgcccacgg tcttgctgcg cccggtgttg cttctgttga tctcagacat ctcacttccc
    16501 cttgtggtcc gcgtcgcttg cgcggcttgt tgtcttggcc cttgttgtga actaccggcc
    16561 ggccccccga aagttggtca gtcctgcggc gcagcacctg cgcgaccgat gtccagccgg
    16621 gtttcgccca ccaggcgatc ccccgcgtag accgccaact cgaccggttc gtccgcgcct
    16681 gccggccgcc gcagcaaaat ggcatagcga tgtttgccgc tatgcagcat gtggatgccc
    16741 ccatcagcca gcttcggcga cgccggcggc gtatcgatcg gcactagtcc ttcgacctcc
    16801 agcgggcggc cgtcgctacg cgccaccagc gtaatcggcc cctccgcctc gacatcgact
    16861 tccatgtaca ggccatgttc ggcctgccgc aagcggagac tccccgagac cgccgcatcc
    16921 tccagccgcc acaccggcgc atccgggccg gccgccggcc gcgagatcgt tcccgccagc
    16981 gatgcagggt cgagctgcgg cacgtcggga tcgcgctgca aaaggaacac cacgccggcc
    17041 gcggtggccg ccagcgcggc gacgagcccg aggccggacc ggcgccgtcc agttgcgaaa
    17101 tcgtctgtcg gggtggcgcg gggtggcaac gcatcaagaa tggtggcgcg catgccggac
    17161 ggcggatcct gggcctgcac ggcggcgagc aggtcgtgca gggaccgcgc ccgcgcgtgc
    17221 tcttgccggg cggtctcgga gacggccagc cagtgctgca gttcctcctg ctccgacgga
    17281 gtgagctccc cgtcgacgtc gccgtgaatc agctcgagaa ttctctggtc catcacggtg
    17341 cgcccgtgcc gtcgtcggcc agctgtccgc gcagcagccg gcgcgcatcg aacagcctgg
    17401 acttcaccgt cttctccgga atgccaagga cgttggcgat gtcctggtag ctgcactccg
    17461 tgaaatggcg cagcgtgatc acggcccggt gctcgggaga cagtgtcatc atggccgcct
    17521 ggacccgccg gtggagctcg gcgttgccgt aggcctcggg cgggccatcg ttctcgtcca
    17581 cgcactccgc cacgtcatcg agcggggcgt agcgctgccg gctgtggagg aagttgatgg
    17641 attcattgat ggcaatccgg taaatccagc tgtagaacct gtgcccgaag tc
//
LOCUS       DAOMPG010000017        17499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1437_length_17499_cov_3.954655, whole genome shotgun sequence.
ACCESSION   DAOMPG010000017 DAOMPG010000000
VERSION     DAOMPG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17499
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_17499_cov_3.954655"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /locus_tag="PLS34_01715"
     CDS             complement(<1..82)
                     /locus_tag="PLS34_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008912304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRP86221.1"
                     /translation="MELLCPAGNLPSLRAAVDAGADSIYLG"
     gene            206..688
                     /locus_tag="PLS34_01720"
     CDS             206..688
                     /locus_tag="PLS34_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRP86222.1"
                     /translation="MPRFPSVDQLLPVLARPLRRLPFPLQRAPLQLALEQLFRARVAE
                     GDLEFLEGRCLAVEIDDLGWRWPIGLQRGRLQVLAPAHPADVTIRGLSPAFFAMAGKL
                     EDPDTLFFQRRLVIEGDTELGLGVKNFLDGLDEEQLPLPARLGLRVVREVDSRLRKIS
                     "
     gene            874..3240
                     /locus_tag="PLS34_01725"
     CDS             874..3240
                     /locus_tag="PLS34_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP86223.1"
                     /translation="MVELTDMQPGARPAARITALLLAVALLAACGGGGSGGGGASRQL
                     LADVPGSSSGVVGQAVAEPPGVRVVDSSGAPVSGATVTFRVEQVGASVSPTTMTTNAD
                     GRARPSSWVLGTVAGPNTLVASAAGISRQVSFSATAAAGPASAIRHASRVEQTGDAGL
                     PVAFPPAVRVADSYDNPVAGATVSFAVQQGGGTISAASAVSDANGIARLESWVLGSEL
                     GLNSLQATLPGIGNLSFSAEALAPLELSIESVQLNQATQTVTGDIAAVAGRPGLLRVV
                     VRASRANTATPDVRLRLFRDGVQLWERTISPPSGSVPLNPSLASLSQTWNIALSAAEV
                     QPGLAVEAVVDPQQQISLPSRENTRFPRGEGQAPILVRDLPPLRVLFIPIQATRHNAT
                     GQIFPSTVESFLASTRLWLPVGALESELRGTPFVTDRDLTDQDEISSLLSDLQAARAM
                     ESAGDRYYHGIMPAVNNIPISGIAFRPTSPSSSFRSALSYDRLPRAAETVAHELGHNL
                     GREHSPCGDVEGADPSFPHANGGIGETGYDIVDGVLRGPSGLSDYMGYCTPRWTSDYT
                     YRNILEWRRNDPLAVGADGGADPMAATLPGKESGLLLWGRINDLGVELNPAFELAARP
                     VLPDADGPQLLRGIAIDGSVLFELSFEGAVVPHARNPTERQFAWFVPLAAAQLGALER
                     IELSSPHGFAQYLARREAQPGGPAVLAATADGAAVQERLPGGELRLRWDAVRNPVAVL
                     RDRRTGQVIGIGRSGELRLAAETAAGLDPEWLFSDGVRSRVVVPVQVQ"
     gene            3237..3920
                     /locus_tag="PLS34_01730"
     CDS             3237..3920
                     /locus_tag="PLS34_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86224.1"
                     /translation="MNIDRAQRAGRPLAMALLLLGGWGCQAPEPTAGAAPAPDPRPVE
                     HGAVLLRGWIGEAGADLEPVIPTAAAPSVIAGERNPYRLRGFDDRGEVLFDLRFGEES
                     LASIAGRPGHHFMLVAPVGAGGSLALAAVELDAAQDRTASRQARWSAAELLDGLRGLR
                     LARRDDGTVHVQWDAQRFELLQLRDPAGGAILALDRDGEVAVTAVGGELEAALSDGVR
                     SAATLLHIP"
     gene            4059..6695
                     /locus_tag="PLS34_01735"
     CDS             4059..6695
                     /locus_tag="PLS34_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRP86225.1"
                     /translation="MTRDDTRRCGRRAGRMFAAVAAVAMLAGCLGSSDNEPVVQPPPP
                     PDRVNAAPGGPVATIRRTTNGIPHISAANLESAAFGMGYAQAQDLVCYLADAFVKARS
                     ERAKYFGPGPGNIHIINDFSYLAQRILSGAAAEYAAMSPESRAMVRGFTAGYNKYVRD
                     TDPGDLPPECRNGPWVVEIEPEDLVAHYRIVAQYASGDLFATGAVFIAVPPGVDPTPA
                     PVVQVVEADTLATIEDTVRFARQAAERSDFTDMGLASNAWGVGSELSETGRGALLGNP
                     HFPYTGVRRFYESQITVPDFINIRGAGLHGTPIPLIGFNENLGWSHTVSTSRRFTVYQ
                     LILKPGDPLTYIKAGVEKPITAETFEIEVNVGAPQTVRFARTFYFSEYGPMLAADAVT
                     GGGLPAWGAAGTAYTYRDANADTNGLLDTWLHMARAGDIDELFAVFQNCGSTLWVNTT
                     GADDQGNAFYIDSSSVPYLSPIALGVIAAKRAASVPYDTLFNQGLTLLDGDNIRDDWV
                     QGQCNGLTPFAQKPKLVRNDFVQNSNDSYWSTNVAQPLEGFSPLYGPERTALNPRTRM
                     GLTMLQNPTDPGFADGAPAGQDGLFGAEELYRVIVNNRSWYAEEFLAELRARCTAAGA
                     TEINLPAGGSRSVASACAVLADWDGVYDLDSVGAHVFRVFIVDYRSKFGAHLTEPFDP
                     ADPVGTPGAPAPLDAGDIANDPMLVSLAAGLDRLDLGGIDYDARLGDVQYFQPSGGAP
                     PGGTPMDLGPSFPWHGGDGTLDGAFNAIGVVNQDTAEDTLLPRVNPPQISSTAGLSAT
                     PGQGWRMARGTSWHFGLEFTDQGPRGFGLVSYSQSVDPDSPFFNDQNARYSDKNFRPL
                     LFTEAEIAANVLPGGEITIRDD"
     gene            6733..8199
                     /locus_tag="PLS34_01740"
     CDS             6733..8199
                     /locus_tag="PLS34_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRP86226.1"
                     /translation="MTPRPFFLVLDQGSHAGKAMVFDGNGRIMAAAERCIGTRRPQPG
                     WVEHDAEEVVSSLRDAVADAVASSGVAGITSAGLAIQRSSVVCWDRNTGEALSPVLSW
                     QDRRNAGWLESQHFDPDRLREITGLVASPHYGASKLRWCLDHLAGVQRALVERRLCCG
                     PLASFVLQRLLLERPCLVDPANASRTLLWDLRSRDWSEVLLAACGVPAGCLPRCVPSR
                     YHFGQLDAAGVRVPLTVATGDQSAALFAHGEPSPGQLFVNIGTGAFVQRVFAGTPGNA
                     PGLLRSLAWQDPSSTVGVLEGTVNGAGAAMQWLAEERGTAVETLVAGADRWLAEITAP
                     PLFLNGVGGLGAPYWVADCPIEFSSPGSLEEATAAVLESVVFLLAVNIEALQAASTGA
                     TLGRAEAIVVSGGMARFDGLCQRLADLSGLVVRRSAAIEATAQGLAWLLGRVPAPTDG
                     REVFFQPRANQPLAQRYARWRGWLESRLAELVGPATRG"
     gene            8231..9172
                     /locus_tag="PLS34_01745"
     CDS             8231..9172
                     /locus_tag="PLS34_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86227.1"
                     /translation="MNAFLALIEFLVAIGILIALVSNYLIINKLWKRRMLREVTESIS
                     LWAALLGLFNAVPFLFLFLLVDHSVAGAAKMAISVVTAVVFVLIGSGLWVAELRGRGF
                     RSLFLRALNLERKESGDLIKQLVQPKGAEQILKILHQLASVDGEVDEREAAMIAQFAG
                     EWRLPVPDDRGDGRSPDLVTLRDSVIDYLKLRPPVRQAGQLWDVLQVLAESDARVTLH
                     EAVALEEIEGMLRKYIAGEELDLSLHEVLIVPQSERQVEAVRTLLPGREEKTLRGGRV
                     FSAGHYYSPRYAEFICQKYIGLGIFSTVVPEHPPVDA"
     gene            9165..9566
                     /gene="crcB"
                     /locus_tag="PLS34_01750"
     CDS             9165..9566
                     /gene="crcB"
                     /locus_tag="PLS34_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRP86228.1"
                     /translation="MPEMALVSLGAALGGMARFGVSELAARWCGRQFPWGTLAVNLSG
                     AFAIGLFIGLAPAGMELGNGPHGPVWQFTGVGFLGAYTTVSSFSLETLSLTHDRKWGR
                     ALANIAVTVSGCLTAAAVGLAAGAWLAGMPA"
     gene            9563..9982
                     /gene="crcB"
                     /locus_tag="PLS34_01755"
     CDS             9563..9982
                     /gene="crcB"
                     /locus_tag="PLS34_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRP86229.1"
                     /translation="MNWSHARSLLSLYLAVGLGSAIGGTLRWWLSVVLHAPPGTAFPW
                     GTLLANVSGSLLIGGYAALIAPPRTRAVSLRLRLFIMTGLCGGYTTFSIFSLETLTLL
                     QLGRHGLAAANVGGSLLLWLMSVWLGYQAVSSMRRAA"
     gene            complement(9946..11619)
                     /locus_tag="PLS34_01760"
     CDS             complement(9946..11619)
                     /locus_tag="PLS34_01760"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HRP86230.1"
                     /translation="MRSRIAPLSPAFLTLLCSLALVVFYNGQFWGTLLAAMPARGAGE
                     LAFVASFGVLLVILFNLFLQAVPATLLKPVLILALVAAAAISHFAGTYGTVIDRHMIA
                     SLLQTDFREARDLLSTSMALRLLLLGLLPSLIVWRIPLARRTWRRASAVRLGTIVSSV
                     ALLVLILWPGYQDYAFFLREHRQLGGMVNPATALIATVKLAMDEPLAAGPPRQVAGDL
                     ARPAALGGGDKPMVMVLVLGETARAANFSLNGYGRDTNPELAALPVISFSNVRSCGTS
                     TAESVPCMFSHLGRAEYSKRAAATAENLLDIAQRAGLAVRWIENNSGCKGVCERVSTE
                     RAAAGAADVHCPDGECPDEVMLHPLQRLLQETSDDSLIVLHMNGSHGPAYFRRYPEGF
                     KTFSPTCDTSEIRSCSPQAVMNTYDNTIVYTDHVLASAISALQAEAERIDAVMLFVSD
                     HGESLGESSTWLHGLPYAVAPDVQKHVPMIVWSSEGYRLRQGLDYDCLHRQSDASISH
                     DHLFHSVLGLLGVRTDIYEPRLDFAVQCTATGSASGSLQAARRIDDTAW"
     gene            complement(11680..12789)
                     /gene="zapE"
                     /locus_tag="PLS34_01765"
     CDS             complement(11680..12789)
                     /gene="zapE"
                     /locus_tag="PLS34_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRP86231.1"
                     /translation="MDPADSASLPVTPLARYRAEIARLGWSHDPAQEDVARRLDDLRA
                     TLVSRPPPRRRLFARARPVAPVRGLYLWGDTGRGKTWLVDLFYKCLPFPGKTRRHFHR
                     FMADVHADLREHRAHADPLALVADGIAARARVICFDEFFVSDIADAMILGTLFEKLFA
                     RGVTLVATSNVPPDALYQDGLQRARFLPAIEQLERHTEVVHIGGDVDYRLRLLERAEI
                     YHQPLGPAAEAGLAALFEASAPADTRNRGELRVLGRSIPVRHRADGVAWFDFEAICGG
                     PRSQSDYIEIARSFHTVLVSGVPQFTAENENEARRFIALVDEFYDRKVKLILSAAVPL
                     ESLYGGRRLAFEFKRTTSRLEEMRAQPYLAAPHLP"
     gene            complement(12789..14741)
                     /locus_tag="PLS34_01770"
     CDS             complement(12789..14741)
                     /locus_tag="PLS34_01770"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRP86232.1"
                     /translation="MNDEPLWKPSAERVAHAQLTQFIKAVSGVLAPNIHGWDELWRWS
                     VEHPEHFWPAVWKFCGIKSSHRWDTVLERPDAMPGAKWFSGARLNFAENLLRHDDSRP
                     ALVWRGEDGRRRVHTHAELHREVARLAGAMRALGVGPGDRVAGFLPNCPEAVIAMLAS
                     ASLGATWSSCSPDFGIQGVLDRFGQIRPRVLFTADGYFYSGKRIDSMERMRGVLQRLE
                     GVERVVLVPFLDSNPDVSTLPGAVAWDDFLSAATTLEYAQLPFDHPLYILYSSGTTGV
                     PKCIVHGAGGTLLQHLKEHRLHTDIGPGDVVFYFTTCGWMMWNWLVSGLATGATLVLY
                     DGNPFHPGPGALWDMAEAEGISVFGTSAKYLAAVEKAGYAPGEHHRLDKLRAVLSTGS
                     PLMPASFDFVYRSIKQDVQLASISGGTDIVSCFALGCPILPVHRGELQCRGLGMAVEI
                     FDDAGRAQTDEPGELVCTRPFPSMPVGFWNDPDGAKYRAAYFERFAGVWHHGDWARLT
                     PHGGLIIYGRSDAVLNPGGVRIGTAEIYRQVEKLDEIVEALAIGQDWGDDQRIVLFVR
                     LRDGVALDEAVQAKIRKTIRDNTTPRHVPAKILEVRDIPRTLSGKIVELAVRNVVHGR
                     PVKNTDALANPAALEEFRNRPELAAD"
     gene            complement(14811..16481)
                     /locus_tag="PLS34_01775"
     CDS             complement(14811..16481)
                     /locus_tag="PLS34_01775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRP86233.1"
                     /translation="MRAKLIVLSCLALLSPAAVAAGDATVSERVDALLAPFGDDTPGV
                     AVGVVRDGRLVFTRSAGMADLGFSVPFTVGTPTNIGSTAKQFTGFALALLHQRGELSL
                     DDDIRRYLPDLPDFGTPVTLRHLASHTSGYREFLNALALAGRRIEKGDWIDADEAIEL
                     ARRQPELQNPPGGEWNYNNTGYLMLAMVIEQVTGRPFDEWVTQEILHPLGMRATVFRT
                     TPDVIIRGGTTGYVKADDGFREARDVGGALGAGSVYTTIGDMARWMQHLGRFELGGAE
                     VRTLMTTPFVLNDGGATKYGLGLIIDEWRGLQRWQHGGGDIGHLAAFHYYPELDAGLM
                     VFANHHDIGAGFTAALAEIFFSEQLAPAAAEQAPPADEDAPFDDALFDRYAGRYELEA
                     APGFVLRFFRDGARYMSQATGQPAVEITPLSASSFQVAGVNARVSFEADEQGRITALT
                     LHQNGAHRAARLPDDELPPAPDFSEFAGRYFSAELETYYAIELAQGQLQLRHRRFGPV
                     ALHQVEGDAFRGGFPVFSVDFERDDDGVVTGLRAGNVRARDIVFRRVD"
     gene            complement(16556..17497)
                     /locus_tag="PLS34_01780"
     CDS             complement(16556..17497)
                     /locus_tag="PLS34_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRP86234.1"
                     /translation="MADHDGKTLSTGIARSVACARQLGNVHVAVFHAPDADAPCAAAA
                     KLEGVSRVLSVRHPANGAPLAAVLAPQIAQLAREHEGGYDYVLGPSTTFGKDVLPRVA
                     ALLGVPQVSDLMEVVDARTFKRPIYAGNVITTVKVPDGIPVLATVRIASFQAVGEAGE
                     APIIAVTVDARLPTHTRYLGLAESGGSDRPDLQSAACVVAGGRGVGSKENFEVVFRLA
                     DKLGAAVGASRAAVDAGYVPNDMQVGQTGKIIAPDLYFAIGISGAIQHMTGIKDAGCI
                     VAINKDGDAPIFEAADFGLVGDLFEILPQLERALDAS"
BASE COUNT         2658 a         5947 c         6147 g         2747 t
ORIGIN      
        1 agccgaggta gatcgagtcc gcgccggcgt ccaccgcggc gcgcagcgac ggcaggttgc
       61 cggcggggca gagcagttcc atgcggtctc ccgggttcgc gcagtgagcg gcgagaatag
      121 ccagccgggc gtggcagggc cttgaccaag atcaagccgg cggtgccggg atgggttatc
      181 ttgcgacagt tgcgcggagg cccccatgcc gagattcccg tctgtcgacc agctgttgcc
      241 ggtgctggca cgaccgctgc ggcggttgcc ttttccgctc cagcgcgcgc cgctgcaact
      301 ggcgctggag cagctgtttc gcgcccgggt ggccgagggc gacctcgagt tcctggaggg
      361 gcgttgcctg gcggtggaga tcgatgacct gggctggcgc tggcccatcg gcctgcagcg
      421 tggccgcctg caggtgctgg cgcctgccca cccggcggac gtcaccattc gcggtctctc
      481 gccggcgttt ttcgccatgg cgggcaagct ggaggatccg gatacgctgt tcttccagcg
      541 ccgcctggtg atcgagggcg acaccgagct cgggctcggc gtcaagaatt tcctcgacgg
      601 cctcgacgag gagcagctgc cgctgccggc tcgcctgggc ttgcgggtcg tgcgcgaggt
      661 ggattccagg ctgagaaaaa tcagttagtt gcgcgcaagt atccgccctg cccggggaat
      721 tgcagcccag ggccctcccg gccgatataa tcctggccag cgttcgtacc gcccggggcc
      781 cggtgaatcc ccggcatctg gcaggtcgaa caccttattc ggacgtcctc cggtctgccg
      841 cggcgcggcg ccggcgcaac gaggaaagaa ttgatggttg aactgaccga catgcagccg
      901 ggcgcacggc cggcggcgcg catcaccgcc ctgttgctgg ccgtcgcgtt gctggcggcc
      961 tgcggaggcg gcggcagtgg cggcggcggg gcttcgcgcc aattgctcgc ggacgtgccg
     1021 ggcagcagca gcggggtggt cggccaggcg gtggccgagc cacccggcgt acgggtcgtg
     1081 gacagcagcg gcgccccggt ctccggcgcg accgtcacct tccgcgtgga acaggtcggc
     1141 gcttcggtgt cgccgaccac catgacgacc aatgccgatg gccgggcccg ccccagcagc
     1201 tgggtactcg gcacggtcgc cggcccgaac accctggtcg ccagcgcggc cggcatcagc
     1261 cggcaggtga gcttctcggc cacggcagcc gccgggccgg ccagcgcgat ccgccatgcc
     1321 agccgtgtcg agcagaccgg tgatgccggc ctgccggtcg cctttccgcc ggccgtgcgg
     1381 gtcgcggatt cctacgacaa cccggtggcc ggcgccacgg tgagcttcgc cgtgcagcag
     1441 ggcggcggca cgatttccgc cgcgtcggcc gtcagcgacg ccaacggcat cgcgcggctc
     1501 gaatcgtggg tcctcggcag cgagctcggc ctgaactcgc tccaggcgac gctgccgggt
     1561 atcggcaacc tgagcttctc ggccgaggcg ctggcgccgc tcgagttgtc gatcgagtcg
     1621 gtgcagctca accaggccac ccagaccgtc acgggcgata tcgcggccgt ggcgggccgg
     1681 ccgggcctgt tgcgcgtcgt cgttcgggcc tcccgcgcca acacggcgac gccggacgtc
     1741 cggctgcggc tgttccgcga cggtgtgcag ctgtgggagc ggaccatttc gcctccgtcg
     1801 gggagcgtgc cgctgaaccc cagcctggcg tccctgagcc agacctggaa catcgcgctg
     1861 agcgccgcgg aagtgcagcc cggcctcgct gtggaggcgg tggtggaccc gcagcagcag
     1921 atcagcctgc cgtcgcgcga gaacacgcgc ttcccgcgcg gcgagggcca ggcaccgatc
     1981 ctggtgcgcg acctgccgcc cttgcgcgtg ctgttcattc ccatccaggc gacgcgccac
     2041 aacgccaccg gccagatctt cccgtccacg gtcgagagtt tcctcgcctc gacgcggctg
     2101 tggctgcccg tgggcgcgct cgagtccgaa ctgcgcggca cgccgttcgt caccgaccgc
     2161 gacctgacgg accaggacga gatcagcagc ctgctgagcg acctgcaggc ggcgcgcgca
     2221 atggagtccg ccggcgaccg ctattaccac ggcatcatgc cggccgtgaa caacattccg
     2281 atctcgggca ttgcgttccg tccgacctcg ccgtccagtt ccttccgcag cgccttgtcc
     2341 tatgaccgcc tgccgcgagc cgcggaaacc gtggcccacg agctcggcca caacctcggg
     2401 cgtgagcaca gcccttgcgg ggacgtggag ggtgcggacc cgtcctttcc gcacgccaac
     2461 ggcggcatcg gcgagacggg ctacgacatc gtcgacggtg tgctgcgcgg cccgagcggg
     2521 ttgtcggact acatgggcta ctgcacgccc cgctggacca gcgactacac ctaccgcaac
     2581 atcctcgagt ggcgtcgcaa cgatccgctg gccgtcggcg cagacggcgg cgccgatccg
     2641 atggcggcga cgctgccggg caaggagtcc ggcctgttgc tgtgggggcg gatcaacgac
     2701 ctgggcgtgg aactgaaccc cgccttcgag ctcgcggcgc ggccggtgct gccggacgcc
     2761 gacggcccgc agctgctgcg cggtattgcc atcgacggca gcgtgctgtt cgagctgtcc
     2821 ttcgagggcg cggtcgtgcc gcatgcgcgc aacccgaccg agcgccagtt cgcctggttc
     2881 gtgccgctgg cggcggcgca gctgggtgcg ctggagcgca tcgagctgag cagcccgcac
     2941 ggcttcgcgc agtacctcgc gcgccgcgag gcgcagccgg gcgggccggc cgtgctggcc
     3001 gctacggcgg atggcgcggc ggtgcaggag cgactgcccg ggggcgagct gcggctgcgc
     3061 tgggacgcag tgcgcaaccc ggtggccgtg ctgcgcgacc gacgcaccgg ccaggtcatc
     3121 ggcatcggcc gcagcggcga gctgcgcctt gcggccgaaa cggcggccgg actcgatccg
     3181 gagtggctgt tctccgacgg cgtccgctcg cgagtcgttg tgccggtgca ggtccagtga
     3241 acattgaccg ggcgcagcgc gcgggccggc cgctggcgat ggcgctgctc ctgctgggcg
     3301 ggtggggctg ccaggcgccc gagcccacgg caggtgccgc cccggcgccg gacccgcggc
     3361 cggtcgagca cggcgccgtg ctgctgcgcg gctggatcgg cgaagcgggt gccgacctcg
     3421 aaccggtcat acccacggcc gctgcgccga gcgtgatcgc cggcgagcgg aatccctacc
     3481 ggctgcgcgg attcgacgac cgcggcgaag tgctgttcga cctccgcttc ggcgaggaat
     3541 ccctcgccag catcgccggc cgccccggtc atcacttcat gctggttgcg ccggtcggcg
     3601 ccggcggttc actggcgctg gccgccgtcg aactggacgc ggcacaggat cgaacggcat
     3661 cccgacaggc acggtggtcg gctgccgagt tgctggacgg tctgcgcggg ctgcggctgg
     3721 cgcgtcgcga cgacggcacg gtgcacgtcc aatgggacgc gcagcgcttc gaactgctgc
     3781 agcttcgcga cccggccggc ggtgcgatcc tggcgctcga ccgcgacggc gaggtggccg
     3841 tgacggccgt cggcggcgag ctcgaggcgg cgctgtcgga cggcgtccgg agtgccgcga
     3901 cgctgttgca catcccatag cccggcacgc attcgcggcc aggaaacccc cgcggcgaga
     3961 ttgacgcggt gtgctttgac cgcacccggg cgctcgatta tcgttgcctt tctcgtgggg
     4021 agccgtcagg cgaaaaccag aacaaaagga catggaatat gactcgtgat gacacgcgcc
     4081 gctgcgggcg gcgcgcagga aggatgttcg ctgccgtggc ggcagtcgcc atgctcgccg
     4141 gctgtctcgg cagctcggac aacgagccgg tcgtccagcc gccgccgccg ccggatcgtg
     4201 tgaacgcggc ccccggtggt cctgtggcca ctattcgccg caccaccaac gggatcccgc
     4261 acatcagtgc cgccaacctc gagtctgcgg ctttcggcat gggttacgcc caggcccagg
     4321 acctggtctg ctacctcgcc gacgccttcg tcaaggcgcg cagcgagcgc gcgaaatact
     4381 tcgggcccgg ccccggcaac atccacatca tcaacgactt cagttacctg gcgcagcgca
     4441 tcctcagcgg cgccgccgcc gagtatgccg ccatgtcgcc ggagtcgcgc gcgatggtgc
     4501 gcggcttcac cgccggctac aacaagtacg tgcgcgacac cgacccgggc gacctgccgc
     4561 ccgagtgccg caatggtcct tgggtagtcg agatcgagcc cgaagacctg gtggcgcact
     4621 atcgcatcgt cgcgcagtac gccagcggcg acctgttcgc cacgggcgcg gtgttcatcg
     4681 ccgtgccgcc gggcgtcgac ccgacccctg cgccggttgt gcaggtcgtc gaggccgaca
     4741 cgctggcgac gatcgaggac acggtgcgct tcgcccggca ggcggccgag cgcagcgact
     4801 tcaccgacat gggtctggcg agcaacgcct ggggtgtcgg cagcgagctg agcgaaacgg
     4861 ggcggggcgc gctgctgggg aatccgcatt tcccctacac cggcgtgcgg cgattctacg
     4921 agagccagat caccgtgccc gatttcatca acatccgcgg cgccgggctg cacggcacgc
     4981 cgatcccgct gatcggcttc aacgagaatc tcggctggtc gcacaccgtg agcaccagcc
     5041 ggcgcttcac cgtgtaccag ctcattctca agccgggcga cccgctgacc tacatcaagg
     5101 cgggcgtgga gaagccgatc actgccgaga ccttcgagat cgaggtcaat gtcggcgccc
     5161 cgcagacggt gcgctttgcg cgtactttct acttctccga gtacgggccg atgctggcgg
     5221 ccgatgcggt caccggcggc ggcctgcctg cctggggcgc cgcggggact gcctacacct
     5281 atcgcgacgc caatgccgac accaacggcc tgctggacac ctggctgcac atggcccggg
     5341 ccggcgacat tgacgagctg ttcgccgtgt tccagaactg cggcagcacc ctgtgggtga
     5401 acaccaccgg cgccgacgac cagggcaacg ccttctacat cgacagcagc tcggtgccct
     5461 acctgtcgcc gatcgcgctc ggcgtcatcg ccgccaagcg tgccgccagc gtgccctacg
     5521 acacgctgtt caaccagggc ctgacgctgc tcgacggcga caatattcgc gacgactggg
     5581 tccagggcca gtgcaacggc ctgacgccct ttgcgcagaa gccgaagctg gtgcgcaacg
     5641 acttcgtgca gaactccaac gacagctatt ggtcgaccaa cgtcgcgcag ccgctggagg
     5701 gcttctcgcc gctgtacggg cccgagcgca ccgccttgaa cccccgtacg cgcatgggcc
     5761 tgacgatgct gcagaacccc accgatccgg gcttcgcaga cggggcgccg gccggccagg
     5821 acgggctgtt cggcgcggag gagctgtacc gcgtgatcgt caacaaccgc agctggtatg
     5881 ccgaggaatt cctcgccgag ctgcgtgccc gttgcacggc cgcaggcgcg acggagatca
     5941 atctcccggc cggcggcagc cgcagcgtgg ccagcgcctg cgcggtcctg gcggactggg
     6001 acggggtgta tgacctcgac agcgtcggcg cgcacgtgtt ccgggtgttc atcgtcgact
     6061 accgcagcaa gttcggcgcc cacctgaccg agcccttcga tccggccgac ccggtcggca
     6121 cgcccggcgc gccggcgccg cttgatgcgg gcgacatcgc caacgacccg atgctggtgt
     6181 cgctggccgc cggcctcgac cgcctcgacc tgggcgggat cgactatgat gcgcgcctcg
     6241 gcgacgtgca gtacttccag ccgtccggcg gcgcgccgcc cggcggcacg ccgatggacc
     6301 tcgggccgtc cttcccgtgg catggcggcg acggcacgct ggacggcgcg ttcaacgcca
     6361 tcggcgtggt caaccaggac acggccgagg acaccttgct cccacgcgtc aacccgccgc
     6421 agatctcgag caccgccggt ctctcggcga cgccggggca gggctggcgg atggcgcgcg
     6481 gcacgagctg gcatttcggc ctcgagttca ccgaccaggg accgcgtggt ttcggcctgg
     6541 tgagctactc gcagtcggtg gatccggact cgccgttctt caacgaccag aacgcacgct
     6601 actcggacaa gaacttccgg ccgctgctgt tcaccgaggc ggagatcgcc gccaacgtgc
     6661 tgccgggcgg cgagatcacc atccgcgacg actgagcgac gtggctggca gggtcaagcc
     6721 gggccggggc gcatgacgcc ccggcccttt tttcttgtcc tggaccaggg cagccacgcc
     6781 ggcaaggcga tggtgttcga cggcaacggc cgcatcatgg cagcggccga gcgttgcatc
     6841 gggacgcgcc gcccgcagcc gggctgggtg gagcacgacg ccgaggaggt cgtgtcctcg
     6901 ctgcgcgacg ccgtggcgga tgcggtggcc tcgagcgggg tggcgggcat cacgtctgcc
     6961 ggcctcgcga tccagcgctc cagcgtggtg tgctgggatc gcaacaccgg tgaagcattg
     7021 tcgccggtgc tgtcgtggca ggaccggcgc aacgccggct ggctcgagag ccagcatttc
     7081 gacccggacc gcctgcgcga gattaccggg ctggtggcct cgccacacta tggcgccagc
     7141 aagctgcgct ggtgcctgga ccatctcgcc ggcgtgcagc gcgcgctggt ggagcggcga
     7201 ttgtgctgtg gtccgctggc gagtttcgtg ctgcagcgcc tgctgctcga acggccctgc
     7261 ctggtcgatc cggcgaacgc atcgcgcacg ctgctgtggg acctccgcag tcgcgactgg
     7321 tcggaagtac tgctggcggc ctgcggcgtg ccggcggggt gcctgccgcg ttgcgtgccc
     7381 agccgctacc acttcggcca gctggacgcg gccggggtcc gcgtgccgtt gaccgtcgca
     7441 accggcgacc agtccgcggc actgtttgcc cacggcgagc cctcgcccgg ccagctgttc
     7501 gtgaatatcg gcaccggcgc tttcgtgcaa cgggtgttcg ccggcacgcc gggcaatgcg
     7561 cccggcctgc tgcgcagcct ggcctggcag gacccgtcgt cgacggtggg cgtgctcgag
     7621 ggcacggtga atggcgccgg cgccgcaatg cagtggctcg ccgaggagcg cggcacggca
     7681 gtggagaccc ttgtcgccgg agccgatcgc tggctggcgg agatcacggc gccgccgctg
     7741 tttctcaacg gcgtcggcgg actgggcgca ccgtactggg tggccgattg cccgatcgag
     7801 ttcagcagcc cgggcagcct ggaggaggcc acggccgcgg tgctggagag cgtcgtgttc
     7861 ctgctggccg tgaacatcga ggccctgcag gccgcatcca ccggggcgac cttggggcgc
     7921 gccgaagcga tcgtggtcag cggtggcatg gcacgtttcg acgggctgtg ccagcggctg
     7981 gcggacctga gcggcctcgt ggtacggcgc tccgccgcca tcgaggccac tgcacaaggc
     8041 ctggcctggc tgttgggccg cgtgccggcg ccgacggacg gacgcgaagt atttttccag
     8101 ccgcgcgcca atcaaccgct ggcgcaacgc tatgcgcgct ggcgcggctg gctggaatcg
     8161 cgcctcgccg aacttgtggg gccggccacg cgcgggtaac ttgcccacag gttggcggcg
     8221 gagcttgacc gtgaatgcct ttcttgcctt gatcgaattc ctggtggcca tcggcatatt
     8281 gattgcgctg gtcagcaatt acctgatcat caacaagctc tggaaacgcc gcatgctgcg
     8341 cgaggtgacc gagagcattt cgctgtgggc cgcgctgctc ggcctgttca atgccgtgcc
     8401 gttcctgttt ctcttcctgc tggtcgacca cagcgttgcc ggtgcggcca agatggcgat
     8461 cagcgtcgtc accgccgtgg tgttcgtgtt gatcggcagc ggcctctggg tggccgaact
     8521 gcgcggccga gggttccgga gcctgttcct gcgggcgctg aacctggagc gcaaggaatc
     8581 cggcgatctc atcaagcagc tggtgcagcc gaaaggcgcg gaacagatcc tgaagatcct
     8641 ccaccagctg gcatccgtgg atggcgaagt ggacgagcgc gaagcggcga tgatcgcgca
     8701 gttcgccggc gaatggcgct tgcccgtgcc ggatgacagg ggcgacggtc ggtcgcccga
     8761 tctcgtcacc ttgcgcgaca gcgttatcga ttacctgaag ctgcggccgc cggtccggca
     8821 ggcgggtcag ctgtgggacg tgctccaggt gctggccgag tcggatgcgc gcgtgacgct
     8881 gcacgaagcc gtcgcgctcg aggagatcga gggcatgttg cgcaagtaca tcgccggcga
     8941 ggaactcgac ctgtcgctgc acgaggtgct gatcgtgccg cagagcgagc ggcaggtgga
     9001 ggccgtgcga acgctgttgc cgggtcgcga ggagaaaact ctgcgtggcg gtcgtgtgtt
     9061 ctccgccggc cattactact cgccgcgcta cgccgagttc atctgccaga agtacatcgg
     9121 gctggggatc ttctcgacgg tggttccgga gcatccgccc gtcgatgcct gagatggcgc
     9181 tggtttctct cggcgccgcc ctcgggggca tggcccgctt cggcgtgtcc gagctggccg
     9241 caaggtggtg cggcaggcag ttcccatggg gcacgctcgc ggtcaacctg agcggtgcat
     9301 tcgcgatcgg actgttcatc ggcctggcgc ccgccggcat ggaattgggc aacggccccc
     9361 acggacccgt ctggcagttc accggcgtcg gattcctggg cgcctacacc accgtctcct
     9421 ccttcagcct ggagaccctg tcgctcaccc atgaccgcaa gtgggggcgg gcactggcca
     9481 atattgccgt gaccgtcagc ggctgcctca cggccgctgc cgtcgggctg gccgccgggg
     9541 cctggctcgc gggcatgccg gcatgaactg gtcgcacgcg cgctctctgc tctcgctgta
     9601 ccttgcggtc ggtctcggca gtgcgatcgg cggaacgctg cgctggtggc tgtcagtcgt
     9661 gctgcatgcg ccgccgggca cggcctttcc ctggggaacc ctgctggcca acgttagcgg
     9721 ctccctgctg atcggcggct atgcggcgct gattgccccc cccaggaccc gcgccgtgag
     9781 cctgcgcctc aggctgttca tcatgaccgg cctttgcggg ggctacacga ccttctccat
     9841 cttcagcctg gagactctca ccctgctgca gctcggccga catggcttgg cggctgcaaa
     9901 cgtcggcggc tcgctgctct tgtggcttat gtcggtctgg ctgggctacc aggcggtgtc
     9961 atcgatgcgg cgggctgcct gaagtgatcc tgacgcgctg cctgtcgccg tgcattgaac
    10021 cgcaaaatcc agcctgggtt cgtagatgtc cgtgcgcacg ccgagcaatc cgagcacgga
    10081 atgaaacaag tggtcgtggg agatcgaggc gtcgctttgc ctgtgcaggc agtcgtaatc
    10141 gaggccttgc cggaggcggt aaccttcgga gctccagacg atcatcggaa cgtgtttctg
    10201 gacgtcgggc gcgacggcat aggggaggcc gtgcagccag gtggaggatt cccccaggga
    10261 ctcgccgtga tccgatacga acagcatgac cgcatcgatc cgctcggcct cggcttgcag
    10321 cgcagagatg gcgctcgcca ggacgtggtc cgtgtacacg atggtgttgt cataggtgtt
    10381 catgacggct tgcggactgc aggatcgtat ttcagaggtg tcgcaggtcg gagagaaggt
    10441 cttgaagcct tcgggataac gcctgaaata ggccggcccg tggctgccgt tcatgtgcag
    10501 cacgatcagt gagtcatcgc tggtctcctg cagcagtcgc tgcagcgggt ggagcatgac
    10561 ttcgtcgggg cactcgccgt ccggacaatg cacatcggcg gcgccggctg cagcacgctc
    10621 cgtggacacg cgctcgcaga cgcctttgca gccggagttg ttctcgatcc agcggacggc
    10681 gagaccggcg cgttgcgcga tgtcgagaag gttctcggcg gtcgccgccg cccgtttcga
    10741 atactcggcg cgtcccaggt gcgaaaacat gcacggcacg gattccgccg tggacgtgcc
    10801 acaggagcga acgttgctga agctgatcac gggcaacgca gccagttcgg ggttggtgtc
    10861 gcgtccatag ccgttcagcg agaaattcgc ggcgcgcgcg gtttcgccca gcaccagcac
    10921 catgaccatc ggcttgtcgc caccgccgag cgctgcgggg cgcgcgaggt cgcccgccac
    10981 ctgcctgggc gggcccgcgg cgagcggttc gtccatggcc agcttgaccg ttgctatgag
    11041 ggcggtggcg gggttgacca tgccgccgag ctgtcggtgt tcccgcagga agaatgcgta
    11101 gtcctggtac ccgggccaca ggatgagcac cagtaatgcg acactggaca caatggtgcc
    11161 gagccgtact gccgatgccc ggcgccaggt gcgcctggca agcggaatcc gccagacgat
    11221 gagcgagggc aataagccca gaagcagcag gcgcaaggcc attgaggtgc taagcaggtc
    11281 gcgagcttcg cggaaatcgg tttgcagcag gctcgcgatc atgtggcggt cgatgaccgt
    11341 gccgtaggtg ccagcaaaat gagagatggc ggcggccgcg acgagcgcca ggatgagaac
    11401 cggcttcagc agtgtcgcgg gaacagcctg gagaaacaag ttgaacagga tgaccagcag
    11461 gacgccgaag gacgcgacaa aggccagttc gccggcgcca cgcgccggca tcgccgcgag
    11521 cagggtgccc cagaactgcc cgttgtagaa gactacgagt gccaggctgc acagcaacgt
    11581 caggaacgcc ggagagagtg gggcaatgcg cgatcgcatg gacgcactct agcggcgcgg
    11641 ccttaaatcg atcttaagcg cgcagcgcca ggcagcggct caaggcaggt gcggcgccgc
    11701 gagatagggc tgagcgcgca tttcctccag tcggctggtc gtgcgcttga actcgaaggc
    11761 cagccgcctg ccgccataca gcgactcgag cggcacggcg gccgagagga tcagcttcac
    11821 cttgcgatcg tagaactcgt cgaccagcgc gatgaaacgg cgcgcctcgt tctcgttctc
    11881 ggccgtgaac tgcggcacgc cggagaccag tacggtgtgg aagctgcggg cgatctcgat
    11941 gtaatcggac tggctgcgcg gacccccgca gatggcctcg aaatcgaacc aggcgacacc
    12001 gtcggcacgg tggcgcaccg ggatggaccg gccgagcacg cgcagctcgc cacgattgcg
    12061 ggtatccgcc ggggcggagg cctcgaacag cgcggccagg ccggcctctg ccgccgggcc
    12121 cagcggctgg tgataaatct cggcgcgttc cagcaggcgc agccggtaat cgacgtcgcc
    12181 gccgatgtgc accacttcgg tgtgccgctc gagctgttcg atcgccggca ggaaccgggc
    12241 gcgctgcagg ccgtcctggt agagcgcgtc gggcggtacg ttggaggtcg ccaccagcgt
    12301 cacgccgcgg gcgaagagct tttcgaacag cgtgccgaga atcatggcat cggcgatgtc
    12361 cgagacgaag aactcgtcga aacagatcac ccgggcgcgc gccgcaatgc cgtcggccac
    12421 cagcgccagc gggtcggcat gcgcgcggtg ctcgcggagg tcggcatgca cgtccgccat
    12481 gaagcggtgg aagtggcgcc gggtcttgcc gggaaagggc aggcacttgt agaacaggtc
    12541 caccagccag gttttgcccc gaccggtgtc gccccacaag tacagcccgc gcaccggtgc
    12601 gaccgggcgg gcgcgggcga acaggcgcct gcgcggtggc gggcgtgaga ccagcgtggc
    12661 gcgcaggtca tcgagtcgtc gcgcaacgtc ctcctgcgca gggtcgtgcg accagccgag
    12721 ccgcgcaatc tcggcgcgat agcgcgccag cggcgtaacc ggcaggctgg cgctgtcggc
    12781 gggatccatc agtccgcggc gagctccggc cggttgcgga actcctccag cgccgccggg
    12841 ttggcgagcg cgtcggtgtt cttcaccggg cggccatgca ccacgttgcg caccgccagc
    12901 tcgacgatct tgccgctcag cgtgcgcggg atgtcccgca cctcgagaat cttcgccggc
    12961 acgtgccgcg gcgtggtgtt gtcgcggatg gtcttgcgga ttttcgcctg cacggcctcg
    13021 tccagcgcga cgccgtcgcg caaccgcacg aacagcacga tgcgctggtc gtcgccccag
    13081 tcctggccga tggccagcgc ctcgacgatc tcatccagct tctccacctg ccggtagatt
    13141 tcagcggtgc cgatgcgcac gccgcccggg ttcagcacgg cgtcgctgcg gccatagatg
    13201 atgagcccgc cgtgcggcgt gagccgggcc cagtcgccgt ggtgccagac gcccgcaaag
    13261 cgctcgaagt acgcagcacg gtatttcgcg ccgtccggat cgttccagaa tcccaccggc
    13321 atggagggga acggccgcgt gcacaccagc tcgcccggtt catcggtctg cgcacggccc
    13381 gcgtcgtcga agatctccac cgccatgccc aggccgcgac attgcaactc gccgcgatgc
    13441 accggcagga tcggacagcc gagcgcgaag cagctgacga tgtcggtgcc gccggaaatc
    13501 gaagccagct gcacatcctg cttgatgctc cggtagacga aatcgaagga cgcgggcatc
    13561 aacggcgaac cggtcgagag gacggcgcgc agcttgtcca gccggtgatg ctcaccgggc
    13621 gcatagccgg ccttctcgac cgccgccagg tacttggcgc tggtgccgaa caccgatatg
    13681 ccttccgcct cagccatgtc ccagagcgcc ccggggcccg ggtggaacgg gttgccgtcg
    13741 tagagcacca gcgtggcgcc cgtcgccagg ccggacacca gccagttcca catcatccag
    13801 ccgcaggtcg tgaaatagaa cactacgtcc cccgggccga tgtcggtgtg cagccggtgc
    13861 tccttgaggt gctgcaacag cgtgccgccg gcgccgtgca cgatgcactt gggcacgccc
    13921 gtcgtgccgg aggaatagag aatgtagagc gggtgatcga aaggcagctg cgcgtattcg
    13981 agcgtggtcg ccgcggacag gaagtcgtcc caggccaccg caccgggcag ggtggaaaca
    14041 tccggattcg agtcgaggaa cggcacgagc acgactcgct cgacaccctc caggcgctgc
    14101 aggacgccgc gcatgcgctc cattgaatcg atccgcttgc cgctgtagaa atagccgtcg
    14161 gccgtgaaca gcaccctggg ccggatctgg ccgaagcggt ccagcacccc ctggatgccg
    14221 aaatccggag agcaggacga ccaggtcgcg ccgaggctgg cgctcgccag catggcaatc
    14281 acggcctcgg gacaattggg caggaagccg gcgactcgat cgcccgggcc gacgcccagg
    14341 gcccgcatcg caccagccag tcgcgcgacc tcgcgatgca gctcggcgtg cgtgtggacc
    14401 cgccgccggc cgtcctcgcc gcgccacacc agggccgggc gagaatcgtc atggcgcagc
    14461 aggttttcgg cgaagttcag gcgcgcgccg ctaaaccatt tcgcacccgg catcgcgtcg
    14521 ggcctctcga gcaccgtgtc ccagcgatgc gaggacttga tcccgcagaa cttccagacc
    14581 gccggccaga agtgctcggg atgctcgacg ctccagcgcc agagctcgtc ccagccatgg
    14641 atgttcggcg ccagcacgcc gctgacggcc ttgatgaact gcgtcagctg cgcgtgcgcc
    14701 acgcgttccg ccgagggttt ccagagaggc tcgtcgttca tgtccgcgtc cggaccatca
    14761 ggcgcacaag gttagcatgc gggccggcgc gtcatggcgc gcgccgctcc tcaatccacg
    14821 cgccggaaca cgatgtcgcg cgcccgcacg ttgccggcgc gcagtcccgt cacgacgccg
    14881 tcatcgtccc gctcgaagtc cacgctgaac accgggaacc cgccgcggaa ggcgtcgcct
    14941 tcgacctggt gcagcgccac cgggccgaaa cggcgatggc ggagctgcag ctggccttgc
    15001 gccagttcga tggcgtagta ggtctccagc tcggcgctga aatagcgccc ggcaaactcg
    15061 ctgaaatcgg gcgccggcgg cagctcgtca tccggcagcc gcgcggcccg gtgcgcgccg
    15121 ttctggtgca gggtcagggc ggtaatccgg ccctgttcat cggcttcgaa gctgacacgc
    15181 gcattgacgc cggcgacctg gaagctgctg gcggacaagg gcgtgatctc caccgccggc
    15241 tggcccgttg cctggctcat gtagcgcgcg ccgtcgcgga agaaccgcag cacgaagccg
    15301 ggcgctgctt cgagctcgta acggccggca tagcggtcga acagggcatc gtcgaacggc
    15361 gcgtcctcgt ccgccggcgg cgcctgttcc gcggccgccg gtgcgagctg ctcgctgaag
    15421 aaaatctcgg ccagtgccgc agtgaacccg gcgccgatgt cgtggtggtt ggcgaacacc
    15481 atcaggccgg cgtccagctc cggataataa tggaacgccg cgagatggcc gatgtcgccc
    15541 ccgccgtgtt gccagcgctg caggccccgc cattcgtcga tgatcaggcc aagaccgtac
    15601 ttggtggcgc cgccgtcgtt gagcacgaag ggcgtggtca tgagcgtgcg cacctcggcc
    15661 ccgccgagct cgaaccgtcc caggtgctgc atccagcgcg ccatgtcgcc gatcgtcgtg
    15721 tagacgctgc cggcgcccag cgcgccgccc acgtcgcggg cctcgcggaa gccgtcgtcc
    15781 gccttgacgt agccggtcgt cccgccgcgg atgatgacgt ccggcgtggt cctgaaaacg
    15841 gtggcgcgca tgccgagcgg atgcaggatc tcctgagtca cccactcgtc gaacggccgg
    15901 cccgtgacct gctcgatcac catcgccagc atgaggtagc cggtgttgtt gtagttccac
    15961 tctccgcccg gcgggttctg cagttccggc tggcgcctgg cgagttcgat cgcttcgtcg
    16021 gcatcgatcc agtcgccctt ctcgatgcgc cgtccggcga gcgccagggc gttcaggaac
    16081 tcgcggtagc cgctggtgtg gctcgccagg tgccgcagcg tgaccggcgt gccgaaatcc
    16141 ggcaggtccg gcaggtagcg gcggatgtcg tcgtcgagcg acagttcgcc gcgctggtgc
    16201 agcaaggcca gcgcaaagcc ggtgaactgc ttggcggtcg agccgatgtt ggtcggcgtg
    16261 ccgaccgtga agggtacgct gaagccgaga tccgccatgc ccgcactgcg ggtgaacacc
    16321 agtcggccgt cccgcaccac gccgacggcg acgccgggcg tgtcgtcgcc gaagggggcc
    16381 agcaacgcgt cgacgcgctc ggacaccgtg gcgtccccgg ctgcaacggc ggccggggag
    16441 agcagggcca ggcatgaaag gacgatcagt ttcgcgcgca tggttgcacc cctggtggac
    16501 ggcggcgacc ggacatcagc ccatcaccgg cggcgccggg ccacggattg tcgagctatg
    16561 aggcgtcgag cgcccgttcc agctgcggca ggatttcgaa caggtccccg accagcccga
    16621 agtcggcggc ttcgaagatc ggcgcgtcgc cgtccttgtt gatggcgacg atgcagcccg
    16681 cgtccttgat gccggtcatg tgctggatgg cgccggaaat gccgatcgcg aagtaaaggt
    16741 cgggagcgat gatcttgccg gtctggccga cctgcatgtc gttcggcacg tagcctgcgt
    16801 cgaccgcggc gcgactggcg ccgaccgcgg ctcccagctt gtcggccagc cggaagacga
    16861 cctcgaagtt ctccttgctg cccacgccgc ggccgccagc caccacgcat gccgcgctct
    16921 gcaggtcggg gcggtcgctg ccgccggact ccgccagccc gaggtagcgc gtgtgggtcg
    16981 gcagcctggc gtccaccgtc accgcgatga tcggcgcctc gccggcctcg cctaccgcct
    17041 ggaaggaggc aatgcgcacg gtggccagca ccgggatacc gtccggaacc ttgacggtgg
    17101 tgatgacgtt gccggcgtag atcggccgct tgaaagtgcg cgcatcgacg acttccatca
    17161 ggtccgagac ctgcggcacg ccgagcagcg ccgcgacacg gggcaggacg tccttgccga
    17221 aggtggtgga cggaccgagc acgtagtcgt agccgccctc gtgctcgcga gcgagctgcg
    17281 cgatttgcgg cgcgagcacg gcggccagcg gcgcgccgtt ggccgggtgg cgcaccgaaa
    17341 gcacccgtga cacgccttcg agcttcgctg cggcagcgca aggcgcgtcc gcatccggcg
    17401 cgtgaaatac cgccacgtgc acgttgccga gctgccgtgc gcatgcgacc gagcgcgcga
    17461 tccccgtgct cagagtcttg ccgtcgtggt cggcgatca
//
LOCUS       DAOMPG010000018        17407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1446_length_17407_cov_4.264292, whole genome shotgun sequence.
ACCESSION   DAOMPG010000018 DAOMPG010000000
VERSION     DAOMPG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_17407_cov_4.264292"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /gene="fliF"
                     /locus_tag="PLS34_01785"
     CDS             <1..1605
                     /gene="fliF"
                     /locus_tag="PLS34_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRP86235.1"
                     /translation="QVGLLLSLALVVALLGAAFTYATRPAMVPVLAGMTERDTADAMD
                     ALSRSGFKFKLDAASGRLLVPASSLHEARIRLATEGLPRAATNGFENLGRESGFGTSR
                     MVETARYHHALEGELARSIMTLHGVDSARVHLALPRPTVFVRDRATPTASVIINLHPG
                     RNLDDAQVAGIVHLVASSVPDLDAQRVTVVDQRGRLLTSPHDQAGPGLTGRDLDYVQR
                     LEESYVRRISELLAPVLGADGFRAQVAADVDFTLVESTRESFDPDSTVLRSEQFYEDV
                     NSVPGGPMGVPGALSNQPPAGGVAMPAAAGVTAGVAAGTRVEGAIVEPPPPPQNTSRR
                     STRNFEVDRTVSHTKQAPASLRRLSVAVVVDHRSATGEEGTPVRQPLSEAEMEHISTL
                     VREAVGFDATRGDSVNVVNASFVPQALPEPMAAPELWREPWVADLVKQGVLALLALII
                     VPLVLRPMLRNLSSHPVGRAAPALAGAPGVPALAGATAGGAPAGVPAKRPGGEDQLKV
                     AQDMVREDPRLVAQVVKQWVASDGNQ"
     gene            1592..2605
                     /gene="fliG"
                     /locus_tag="PLS34_01790"
     CDS             1592..2605
                     /gene="fliG"
                     /locus_tag="PLS34_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRP86236.1"
                     /translation="METSKAANLSGTDRAAVLLMSLGEENAAGVLKHMGPKEVQRIGT
                     AMASLQSISRQDMNRVLQDFVQTVEHQTALGVGSDEYVRKVLTSALGEEKAGGVIDRI
                     LTGRNSKGLEALKWMDARAVADMIRMEHPQIVAIVLSFLDPDQAAEVLALLPEKSQSE
                     IVMRIATLDTVQPNALEELDQILEKQFSSSNAQQPAAMGGVKNAANIMNYLDPSIESR
                     IIEQIRKSDAALGGTIQDLMFTFDNFLDLDDRSVQALLREVPGDRLVIALKGADEAVR
                     EKIFRNMSKRAAEMLREDLESKGPVRLSDVEAAQKEILGTVRRMAENGEIVIGGRGGD
                     EYV"
     gene            2598..3254
                     /locus_tag="PLS34_01795"
     CDS             2598..3254
                     /locus_tag="PLS34_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HRP86237.1"
                     /translation="MSEDGAGSALARAGGILSGAEARPWAVPAVGRSAGGHGGPLTAG
                     RLESIEEEARESGRAAGHQEGYAAGQAEVMRRVEQLEQIIAGLAQPVSHLDAEVEQEL
                     LEMVMAIARRLIRRELKTNPGEIIGVVREGIASLPIGERKVTLQLHPEDAALVRDVFG
                     MNEGGPACRIVEDPSLSRGGARISTDISIIDATLETRISRVFDRLLGGERQADEGDAE
                     "
     gene            3251..4630
                     /gene="fliI"
                     /locus_tag="PLS34_01800"
     CDS             3251..4630
                     /gene="fliI"
                     /locus_tag="PLS34_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRP86238.1"
                     /translation="MTQVVPLPAPEQRRTALCASLERRRRRVQQAEPMIAEGKLVRMV
                     GLTLEAVGCEAPVGSRCMIVGGGGAQTEAEVVGFAGERVFLMPVGDIHGLTPGARVRP
                     LSGSRNTPVGEGLLGRVVDGSGRPLDGRGPVECDAWMPLRGRAVNPLSRAPLEQPLDV
                     GVRAINGLLTVARGQRMGLFAGSGVGKSVLLGMMTRYTAADVTVVGLIGERGREVNDF
                     VQKTLGPEGMKRAVVVATPADHPPLMRLHGAMLATTIAEYFRDRGLNVLLLMDSLTRF
                     AQAQREIGLAIGEPPATKGYTPSVFARLPQLVERAGNGEPGGGSITAFYTVLAEGDDT
                     NDPIADAARAVLDGHVVLSRRVAEGGRYPAIDIEASISRAMNDICAPAHRDAAQRFRR
                     LYATWSQHRDLLSVGAYKAGSDPAVDEAVAYQPQLEGWLRQDMHDAVPLVQSLQSLDK
                     VVAPLETEV"
     gene            4635..5081
                     /gene="fliJ"
                     /locus_tag="PLS34_01805"
     CDS             4635..5081
                     /gene="fliJ"
                     /locus_tag="PLS34_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRP86239.1"
                     /translation="MTESLRSSRLEPIARIADHREQAAARDFGRLQEVLVAQEQRLAE
                     LRAWWQEYADGLDKQSGRGSGLMREGRLFLAQLNDAIERQRGMVEYARRDLEAARARW
                     LASRMDHEALDKVVARCKDEESRRARRGEQREQDECAARSTRPEIT"
     gene            5170..6168
                     /locus_tag="PLS34_01810"
     CDS             5170..6168
                     /locus_tag="PLS34_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRP86240.1"
                     /translation="MLDMPPTLAEADAARAPTPRAAGAEPGNAGDTAQPFALLLAAGE
                     PVDPAAWSRLMSGAGDGAPETATEEALAAALAAAIESGKFVPPAGEFLPPGHMIRAPA
                     GLPGDPAAPDDAVLTRMIDMLRTLQGHAGQALPTGAGEMPMQPGLEMKVVEQFDSARL
                     LSTTLGPQAQAPVSAASPATTGTTPAAATPPSVPIAVPPGRPDWSAAVGQRVLWMVSH
                     KTQAAELRLNPPELGPVEVKVRTDDDGVRLSFAAGNAAVREALESAAPRLREMFLAEG
                     LRLENMDIGQRHAGAGRDDGSGGIAGPDDATGEDVEAQASERSAQPQRAGLVDCFV"
     gene            6347..6880
                     /locus_tag="PLS34_01815"
     CDS             6347..6880
                     /locus_tag="PLS34_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRP86241.1"
                     /translation="MINPEAEGFDEQLDAAAAAGVAGPRRGKGRMVILLVVLAALGGG
                     GWFAWQTFMVEKAADGALGSADAPGSRSNANYLPLDPPLVVNFAAGGQLRYLQVSVEV
                     MARDARAIGIIDRHAPAVRNSLIALFSERDYEALLTRAGKEALRQEALEVVRSELTML
                     TGQPQAEDVYFSSFVMQ"
     gene            6882..7907
                     /gene="fliM"
                     /locus_tag="PLS34_01820"
     CDS             6882..7907
                     /gene="fliM"
                     /locus_tag="PLS34_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRP86242.1"
                     /translation="MSKEDLLSQDEIDALLHGVDSGDVSTGDDLRARDGVARPIDLAS
                     QERIMRGRMPALDMTNERFARHLRNSMFNLLRRPVEVTVGEVRLEKFSEYLHGLPVPT
                     SLNMIRVKPLRGTALLVMDACLVFAMVDHFFGGAGRFNAKVEAREFTRTETRVMRMVL
                     DLVFKGLAEAWEPVQALEFEYLKSEANPQFADVVRPNELMIVCSFKLQLEGGGGELHF
                     ALPYSMIEPIRHLLDARPPAERSEGDGRWQKSLRDEMRLAPVEVSCKLCEIELSVAEL
                     LRLRPGDVLPMDMPERVTLYAEGIPAFHGRFGASQGRHAVQVIEPARREHAGADIKRE
                     LSQERGR"
     gene            7904..8332
                     /gene="fliN"
                     /locus_tag="PLS34_01825"
     CDS             7904..8332
                     /gene="fliN"
                     /locus_tag="PLS34_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019022109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRP86243.1"
                     /translation="MSERQTQASTEETGAARAGAAAAAADGGAGQGPRTAKAGTPVPP
                     KPLKDEGGAAGEDVNLEVVLDIPVTLSMEIGRAKLPIRNLLQLNQGSVVELERMVGEP
                     MDVLVNGTLIAHGEVVVVNEKYGIRLTDVISPAERVRKLR"
     gene            8340..8771
                     /gene="fliO"
                     /locus_tag="PLS34_01830"
     CDS             8340..8771
                     /gene="fliO"
                     /locus_tag="PLS34_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRP86244.1"
                     /translation="MKRLLTLLAVLLLPLAAAGAEPAAAPGFAGAGQVGKMIAGLLLM
                     IVIIIGLAALLKRVNGLQRSLPGAIRVVSVTSIGARERLMLVEVGGRQLLVGAAPGRV
                     QTLLVLDQPVAMPQTAAGRQASGFAERLRDALGAPAKEARS"
     gene            8810..9541
                     /gene="fliP"
                     /locus_tag="PLS34_01835"
     CDS             8810..9541
                     /gene="fliP"
                     /locus_tag="PLS34_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583574.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRP86245.1"
                     /translation="MLAWPLSAGAETAGGNASGLELLSVSTAEDGSQQYSVTLQVLLL
                     MTLLSLLPAALIMMTSFTRIIVVLAILRQGMGTQQTPNNQILIGLALFLSMFVMAPVL
                     ERIYEDAARPYLEETLNAPAALQAASGPIREFMLSQVRTPDLQLFAGLGGHEGFEAPE
                     DVPFSVLVPAFVTSELKTAFQIGFLLLVPFLIIDLVVASVLMSMGMVMLSPLIISLPF
                     KIMLFVLVDGWSLVMSTLAASFMVS"
     gene            9545..9817
                     /gene="fliQ"
                     /locus_tag="PLS34_01840"
     CDS             9545..9817
                     /gene="fliQ"
                     /locus_tag="PLS34_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRP86246.1"
                     /translation="MSPELVMEVGRNALAVTVMLAAPMLVVALALGLFIGMIQAATQV
                     QEMTLSFIPKLLGLVAVLAIGGHWMLGLIVDYTRELITAIPGLLLG"
     gene            9829..10599
                     /gene="fliR"
                     /locus_tag="PLS34_01845"
     CDS             9829..10599
                     /gene="fliR"
                     /locus_tag="PLS34_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRP86247.1"
                     /translation="MEFTQLQLDTWISSLVLSFTRIAALLIAAPLFGGRSLPMRVRVS
                     FALVLSMMVLPILPPAPPLELLSFPMVLAVGRELLIGAMAGFILQLVFAALAIAGEVI
                     ALGMGLGFASLVDPDKGVQVPVVGQLLVIMATLLFLAMDGHLALVSFMVLSFTGVPIG
                     GGISFENFAAIAGWGSVMFGHAVMIALPAVGALLLANLAMGVVTRAAPQLNIFAVGFP
                     MTLMLGFVLLYATVGTIGGRFAELLAEGFGMIGWLLGG"
     gene            10604..11749
                     /gene="flhB"
                     /locus_tag="PLS34_01850"
     CDS             10604..11749
                     /gene="flhB"
                     /locus_tag="PLS34_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRP86248.1"
                     /translation="MAEQDKSQERTEQATPKRRQDAKKKGQAPRSRELNTTAVLLAGA
                     GTLVLFGGAIGEGIAAIMIDSLSPSRELAFDAPLLPTVFGQSAMVALVLLLPLFAAVL
                     IAALAGPAMLGGLVFSAQALQPKLEKLNPIAGLKRIFSLNGLMEFAKTLAKFVVVTGA
                     GLLVLRHIAPDLLMAGEASIETAVPGLAAAAAWVLVVLASVMLLIAAVDVPFQLWNHS
                     KQLRMTRQEVRDEMKETEGRPEVKGRIRRVQMEMTQRRMMMAVPEADVVVTNPTHYSV
                     ALKYDPDGMAAPRVVAKGADQIALKIREVAAASGVPRVEAPPLARALYFTTRLDEEIP
                     AELFLAVARVLAWVYQLKAAAGAAPPPQPEGLDVPDHLTRRQPGRRI"
     gene            11752..13845
                     /gene="flhA"
                     /locus_tag="PLS34_01855"
     CDS             11752..13845
                     /gene="flhA"
                     /locus_tag="PLS34_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRP86249.1"
                     /translation="MGNLATGFGTILRTGLGTPLLLVALLVMIVLPLPPIALDVFFTF
                     NIALALVVILMTVYAPRPLEFSVFPTVLLLATLLRLALNVASTRVVLIDGHTGTDAAG
                     KVIEAFGDFVVGGNYAVGLVVFAILVIINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNSGLITQDEARARRAETAAEADFYGSMDGASKFVRGDAVAGILILFINIIGGL
                     IIGTVTHDMALADASRTYILLTIGDGLVAQIPALLLSSATAIIVTRVSAAGDMGRDVV
                     RQLFGSARAIGVAAAIIGMLGLVPGMPNLVFLGLAAALGTAAWMLHKRSRQTEVEVLP
                     EESAEPAAPRELAWDDVSQVDPIGMEVGYRLIPLVDRAQGGVLLERMKGVRRKLSEEL
                     GFLVPPVHVRDNLDLGPNGYRITLNGVPAARGEIHADRHLAINPGRVYGQIAGTETTD
                     PAFGLPAVWVAAEQRDQAQTLGYTVVDPATVVATHLSQVLRDHAHELLGHDDAQQLLD
                     RLAKSHPKLVENLVPKVLSLGVVLRVMQNLLAEQIPIRDMRTIAETLAEHGARSQDPV
                     ALTAAVRTALARFIVQGITDSHEDLPLIALDPGLEQVLQRSLQATGSAGLGIEPRLAE
                     RLQRGASEAAQRQEIAGEPAVLVTSAELRPWLARWLRPVVRGLHVLAYTEIPDSRPVK
                     VVATLGAAEAQAATA"
     gene            13874..15181
                     /gene="flhF"
                     /locus_tag="PLS34_01860"
     CDS             13874..15181
                     /gene="flhF"
                     /locus_tag="PLS34_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRP86250.1"
                     /translation="MKIKRFFAPDMRQAIRMVRDEQGPDAVILSTRNTGGGVEIVAAI
                     DYDAELVNGMLGEPQTEQRDAHSAPPHPAEAAARAEVLPEPAAPAVPPATEALPEPEI
                     AEVPELLKRSAPSRTPRVEWTQDPTLAAMQDELKSLRAILQDQVTRLATADYDRREPV
                     RAEIARRLMKAGFEEAVARGVAAGTTSLATPAEAWREAQFGLAQRLPVVATDPVEQGH
                     VIALIGATGVGKTTTAAKLAARACLRYGSKSVALISTDDFRVGAQRQLASFGLLLGLP
                     VRQARSTAELADQLAEFDNRRLVIIDTAGMGQRDVRLIEETRKLSQVAELQSYLVMSA
                     NLQREVMDEVVDAFGRDQLAGCILTKLDEAARLGPALSTLLTHQLPVAFVSEGQRVPE
                     DLRPARARDLVSRALASPARAARPGPSPTAAAGTVQEVRHASA"
     gene            15168..16058
                     /locus_tag="PLS34_01865"
     CDS             15168..16058
                     /locus_tag="PLS34_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRP86251.1"
                     /translation="MHQPELARQANGLASFRQPRPVRVLAVASGKGGVGKTNVSVNLG
                     VAFAGLGRRTLLLDADLGLANVDVLLGLQVPYNLSHLLGGERELDEIIVSGPAGLRIV
                     PASSGVARLANLSPAEHAGIIGAFSSLAEPVDVLIVDVAAGISPAVLQFCVAAQEVMV
                     VVCDEPSSLTDAYALVKVLARDHGVRRFHTITNRVDSPHHGRRLHHKLQSVCDRFLDI
                     PLQHLGAVPEDPLLRRAVQMQAPVVTAYPSSPAARAFKDLARHADTLPEPRGMSGSIE
                     FFLERLAGQDPREDGLEAVG"
     gene            16061..16786
                     /locus_tag="PLS34_01870"
     CDS             16061..16786
                     /locus_tag="PLS34_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HRP86252.1"
                     /translation="MYAAAREFDGEHVVERYLGLVKRIACHLLGRLPANVQLEDLVQS
                     GTIGLLEAARHYDAGQGASFETYAGIRIRGAMLDEVRRNDWTPRSVHRNARRVSAAIR
                     AVESETGREAQPREIAAVLEVGLDEYHAMARDTVAARLFSLNDLTEEDGSFEDMLPGE
                     EAGPAEEFDREAFRRDLAVAISGLPEREQLVMSLYYEQEMNLREIGAVLGVTESRVCQ
                     LHGQALARIRARLRNWTDDEGMD"
     gene            16898..>17407
                     /locus_tag="PLS34_01875"
     CDS             16898..>17407
                     /locus_tag="PLS34_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HRP86253.1"
                     /translation="MDVLSVAGLALGVVAILGGSILKGSGLAALWNGAAFVIVIVGTL
                     AATLLQVRLETFKHAMKMFRWIIQPPANDPAQAITKIVSWSQVARKEGLLGLESTAEE
                     EPDEFLQKGLQLLVDGSEPEALRGILEVDISTREEFDNRGAKVFESMGIYAPTLGIIG
                     AVLGLIAVMG"
BASE COUNT         2607 a         5991 c         6269 g         2540 t
ORIGIN      
        1 caggtcggcc tgctgctgag tctcgcgctg gtggtggcgc tgctgggcgc cgccttcacc
       61 tatgcgaccc gccccgccat ggtcccggtg ctggccggga tgacggagcg cgacaccgcc
      121 gacgccatgg acgcgctcag ccgcagcggc ttcaagttca agctggacgc cgccagcggg
      181 cgcctgctgg tgccggcgag cagcctgcac gaggcacgca tccgtctcgc caccgagggg
      241 ctgccgcgcg cggccaccaa cggcttcgaa aacctcggcc gcgaatccgg cttcggcacc
      301 agtcgcatgg tcgagaccgc gcgctaccac cacgccctgg agggcgaact ggcgcgttcc
      361 atcatgaccc tgcacggcgt ggacagcgcc cgcgtccacc tcgccctgcc gcggcccacg
      421 gtgttcgtgc gggatcgcgc caccccgacg gcctcggtga tcatcaacct gcatcccggc
      481 cgcaacctcg acgacgccca agtggccggc atcgtgcacc tggtggcgtc cagcgtgccg
      541 gacctcgacg cccagcgcgt caccgtggtc gaccagcgcg gccgcctgct cacctcgccg
      601 cacgaccagg ccggacccgg cctgaccggt cgcgacctgg actacgtgca acggctcgag
      661 gagagctacg tgcgccgcat ctccgagctg ctggcgcccg tgctgggggc cgacggcttc
      721 cgcgcacaag tggcggccga cgtcgacttt acgctggtcg agagcacccg cgagtccttt
      781 gacccggaca gcaccgtgct gcgcagcgag cagttctacg aggacgtgaa cagcgtgccg
      841 ggcggcccga tgggggtgcc cggggcgctc agcaaccagc cgccggcggg cggggtggcg
      901 atgcccgccg cggccggcgt gacggccggc gtcgccgcgg gcacgcgggt cgagggggcg
      961 atcgtcgaac cgccgccgcc gccgcagaac accagtcgcc gttccactcg caacttcgag
     1021 gtcgatcgca ccgtcagtca caccaagcag gcgccggcca gcctgcgcag gctgtcggtg
     1081 gcggtggtgg tcgaccaccg cagcgccacc ggcgaggaag gcacccccgt gcgccagccg
     1141 ctctccgagg cggagatgga gcacatctcg acgctggtgc gcgaggccgt gggcttcgac
     1201 gcgacacgcg gcgacagtgt caacgtggtc aatgcgtcgt tcgtgccgca ggccctgccg
     1261 gagcccatgg cggcgccgga gctgtggcgc gagccgtggg tcgccgacct ggtgaagcag
     1321 ggtgtgctgg cgctgctggc gctcatcatc gtgccgctgg tgttgcggcc gatgctcagg
     1381 aatctctcca gccatccggt cgggcgcgcg gcgcccgccc tggccggggc gccgggtgtc
     1441 ccggcgctgg ccggcgcaac ggccggcggc gcccccgcgg gtgtgccggc caagcgcccg
     1501 ggtggcgagg accagctgaa ggtcgcgcag gacatggtgc gcgaggatcc caggctggtg
     1561 gcacaggtcg tgaagcagtg ggtggcgtcc gatggaaacc agtaaggcag cgaacctgtc
     1621 cggcaccgat cgcgcggccg tgctgctgat gagcctcggc gaggagaacg ccgccggcgt
     1681 gctcaagcac atgggcccga aggaagtgca gcgtatcggc acggccatgg cctcgctgca
     1741 gagcatttcc cggcaggaca tgaaccgcgt actgcaggat ttcgtgcaga cggtggaaca
     1801 ccagaccgcg ctcggcgtcg gcagcgacga atacgtgcgc aaggtgctga cctccgcgct
     1861 gggcgaggaa aaggccggcg gtgtcatcga tcgcatcctc accgggcgga actcgaaggg
     1921 cctggaggcg ctgaagtgga tggacgcccg cgcggtggcc gacatgatcc gcatggagca
     1981 tccgcagatc gtggccatcg tgctgtcctt cctcgacccc gaccaggccg ccgaggtgct
     2041 ggccctgctg ccggagaagt cgcaatcgga gatcgtgatg cgcatcgcca cgctggacac
     2101 cgtccagccg aatgcgctgg aggagctcga ccagatcctc gagaagcagt tctccagcag
     2161 caacgcccag cagcccgcgg ccatgggcgg cgtgaagaat gccgccaaca tcatgaatta
     2221 cctcgacccg tccatcgagt cgcgcatcat cgagcagatc cgcaagtcgg atgccgcgct
     2281 cggcggcacc atccaggacc tgatgttcac cttcgacaac ttcctcgacc tggacgaccg
     2341 ctcggtgcag gcgctgctgc gcgaagtgcc gggcgaccgg ctggtcatcg cgctcaaggg
     2401 cgcggacgag gcggtgcgcg agaagatctt ccgcaacatg tccaagcgcg ccgccgagat
     2461 gctgcgcgag gacctggagt cgaagggccc ggtgcggctg agcgacgtcg aggccgccca
     2521 gaaggaaatt ctcggcacgg tgcgccgcat ggcggagaac ggcgagatcg tgatcggcgg
     2581 ccgtggcggg gacgagtatg tctgaggacg gcgcaggatc ggcgctcgcc agggcgggcg
     2641 gaatcctctc cggcgccgag gcgcgtccgt gggccgtgcc cgccgtgggg cggtcggccg
     2701 gcgggcatgg cgggccgctg accgcgggcc gcctggagag catcgaggag gaagcgcgcg
     2761 agagcggccg ggcggcgggc caccaggagg gctacgcggc cggacaggcc gaggtgatgc
     2821 ggcgggtgga gcagctcgag cagatcatcg ccggactggc gcagccggtc agccacctgg
     2881 acgccgaggt cgaacaggag ctgctggaga tggtgatggc cattgcgcgg cgcctgatcc
     2941 ggcgggagct caagacgaat cccggcgaga tcatcggcgt ggtgcgggag ggcatcgcat
     3001 cgctgccgat cggcgagcgc aaggtgacgc tgcagcttca cccggaagac gccgccctcg
     3061 tcagggacgt gttcggcatg aacgagggcg gccccgcctg ccgcatcgtc gaggatccgt
     3121 cgctgagccg cggcggggcg cggatctcga ccgacatatc gatcatcgac gccacgctgg
     3181 agacccgcat cagccgggtg ttcgaccgcc tgctgggcgg cgaacgccag gccgatgaag
     3241 gggacgccga atgacgcagg tcgtgcccct gcccgctccc gagcagcgcc gtaccgcgtt
     3301 gtgcgccagc ctcgagcgtc gtcgccggcg cgtgcagcag gctgagccga tgatcgcgga
     3361 gggcaagctg gtgcgcatgg tcgggctgac gctcgaggcg gtcggttgcg aggcgccggt
     3421 gggcagccgc tgcatgatcg tcggcggcgg cggcgcgcag accgaggccg aggtggtcgg
     3481 cttcgccggc gagcgcgtgt tcctcatgcc ggtgggcgac atccacggcc tgacgccggg
     3541 cgcgcgcgtg cggcccctgt ccggttcgcg caacacgccg gtgggagaag gcctgctggg
     3601 ccgcgtggtg gacggctcgg gccggccgct ggacgggcgc ggcccggtgg aatgcgacgc
     3661 ctggatgccg ctgcgcggcc gggcggtcaa tcccctgtcg cgcgcgccgc tggagcagcc
     3721 gctggacgtc ggcgtccggg ccatcaacgg cctgctgacg gtggcgcgcg gccagcgcat
     3781 gggcctgttc gccggcagcg gcgtcggcaa gagcgtgctg ctcggcatga tgacccgtta
     3841 caccgcggcg gacgtcacgg tggtggggct gatcggcgag cgcggccgcg aggtgaatga
     3901 tttcgtgcag aagaccctcg gcccggaggg catgaagcgc gccgtggtcg tggcgacgcc
     3961 ggcggaccat ccgccgctga tgcgcctgca cggcgccatg ctggccacca ccatcgccga
     4021 gtattttcgc gaccgcggcc tgaacgtcct gctgctgatg gattcgctga cgcgcttcgc
     4081 ccaggcgcag cgcgaaatcg gcctcgccat cggcgagccg cccgccacca agggctacac
     4141 gccgtcggtg ttcgcgcgcc tgccgcagct ggtggagcgc gcgggcaacg gcgagccggg
     4201 cggcggctcg atcaccgcct tctacacggt gctggccgag ggcgacgaca ccaacgaccc
     4261 gatcgccgat gcggcgcgcg cggtgctgga cggccacgtg gtgctgtcgc gccgcgtggc
     4321 cgagggcggc cgttacccgg ccatcgacat cgaggcctcc atcagccggg ccatgaacga
     4381 catatgcgca cccgcgcacc gcgacgccgc acaacggttc cggcgcctct acgcgacctg
     4441 gtcgcagcat cgcgacctgc tgagcgtcgg cgcctacaag gcgggctccg atcccgccgt
     4501 ggacgaggca gtggcctatc agccgcagct ggagggctgg ctgcgacagg acatgcacga
     4561 tgccgtgccc ctggtgcaga gcctgcagtc gctggacaag gtggtcgcgc cactggaaac
     4621 agaggtctag ggacatgacc gaatcattgc gcagcagccg tctcgagccg atcgcccgga
     4681 tcgcggacca tcgtgagcag gcggccgcgc gggacttcgg ccgcctgcag gaagtcctcg
     4741 tcgcgcagga gcagcgcctg gccgaactgc gcgcctggtg gcaggagtac gccgacggcc
     4801 tggacaagca gtccggcagg ggctcgggcc tgatgcgcga gggacggctg ttcctcgcgc
     4861 agttgaacga cgccatcgag cgccagcgcg gcatggtcga atacgcccgg cgggacctgg
     4921 aggcggcacg tgcgcgctgg ctggcgtcgc gtatggacca cgaggccctg gacaaggtgg
     4981 tggcgcgctg caaggacgag gaaagccgcc gcgcccgccg tggcgagcag cgcgagcagg
     5041 atgaatgcgc cgcacgcagt acccggccgg aaatcacgtg aggcgccggg accggccagg
     5101 tggcacggat cctgcagccg gaatgacatc ggcggcgctt gcgcgccgcg cccggagaac
     5161 gacgccatga tgctggacat gcccccgacc cttgccgagg ccgacgcggc acgtgctcca
     5221 acgccgcggg ccgcgggcgc ggaacccggc aatgcagggg atacggcgca gcctttcgcc
     5281 ctgctgctgg cggccggcga gcccgtcgac ccggccgcgt ggtcgcggct gatgagcggc
     5341 gccggggacg gcgcgccgga aacggcgacg gaggaggcgc tcgcggcggc gctggccgcg
     5401 gccatcgaaa gcggcaagtt cgtgccgccc gccggcgagt tcctgccgcc cggacacatg
     5461 atccgcgcgc cggccggcct gccgggcgac ccggcggcgc cggacgacgc cgtcctgaca
     5521 cgcatgattg acatgctgcg caccctgcag ggccatgcgg gccaggcgct gccgacagga
     5581 gccggcgaga tgcccatgca gccgggcctc gagatgaagg tcgtcgagca gttcgactcc
     5641 gcccgcctgc tgtcgacgac gctgggcccc caggcccagg cgccggtgtc ggcggcgtca
     5701 ccggcgacga ccggcacgac gccggccgcc gccacgcccc cgtccgtgcc gatcgccgtg
     5761 ccgcccggcc gaccggactg gagcgcagcc gtcggccagc gggtgctgtg gatggtttcc
     5821 cacaagaccc aggcggccga gctccgcctc aacccaccgg aactcggccc ggtcgaggtc
     5881 aaggtcagga ccgacgacga cggcgtccgg ctgagcttcg cggcgggcaa tgcggcggtg
     5941 cgcgaagcgc tcgaatcggc tgcgccgcgc ctgcgcgaga tgttcctcgc cgagggcctg
     6001 cgcctcgaga acatggacat cggccagcgg catgccggcg ccggccgcga cgacgggtcc
     6061 ggcggtatcg ccggcccgga cgacgccacg ggcgaggatg tcgaggccca ggcatccgag
     6121 cggtcggccc agccgcagcg cgccggcctg gtggattgct tcgtctagcc ccgtggcggc
     6181 cgaccggtcg cacgcacttc tgacgccgcc gcgccggcag gcgtcaagac tctgacggcg
     6241 cgggatttgg tcgctccaac tacctgaatt taaaggtctc ttccggctgg cacggctctt
     6301 gctccagcta ttcgcgagtt attcgcgacc gcatggagtg cctcagatga tcaacccgga
     6361 agccgaaggc ttcgacgagc agctggacgc cgctgcggcg gcaggcgtgg ccggaccccg
     6421 gcggggcaag ggccgcatgg tgatcctgct ggtggtgctg gcggccctcg gcgggggcgg
     6481 ctggttcgcc tggcagacct tcatggtgga gaaggccgcg gacggcgcgc tcggcagcgc
     6541 cgacgcgccg ggcagccgca gcaacgccaa ctacctgccg ctcgacccgc cgctggtggt
     6601 gaacttcgct gccggcggac agctgcgcta cctgcaggtc tcggtcgagg tgatggcgcg
     6661 cgatgctcgt gccatcggca tcatcgatcg ccacgcgccg gccgtgcgca attccctgat
     6721 cgcgctgttc agcgagcggg actacgaggc gctgctgact cgggccggca aggaagcgtt
     6781 gcgccaggaa gccctcgagg tcgtgcgctc tgagctgacc atgctgaccg gtcagccgca
     6841 ggccgaggac gtctacttca gcagcttcgt gatgcagtga catgagcaag gaagacctgc
     6901 tttcccagga cgagatcgac gcgctgctgc acggcgtcga cagcggcgac gtgtccaccg
     6961 gcgacgacct gcgcgcgcgc gacggcgtcg ctcgtcccat cgacctggcc agccaggaac
     7021 gcatcatgcg gggccgcatg ccggccctcg acatgaccaa cgagcggttc gcgcgacacc
     7081 tgcgcaacag catgttcaac ctgctgcggc ggccggtgga ggtcaccgtc ggcgaggtgc
     7141 ggctggaaaa gttcagcgaa tacctccatg ggctgccggt ccccaccagc ctgaacatga
     7201 tccgcgtgaa gccgctgcgc ggcacggcgc tgctggtcat ggacgcatgc ctggtgttcg
     7261 ccatggtgga ccacttcttc ggcggcgccg gccgcttcaa cgccaaggtc gaggcgcgcg
     7321 agttcacgcg caccgagacg cgcgtcatgc gcatggtgct ggacctcgtc ttcaagggcc
     7381 tcgcggaggc ctgggagccg gtgcaggcgc tggaattcga atacctgaag tcggaggcca
     7441 acccgcagtt cgcggacgtc gtccgcccca acgagttgat gatcgtctgc agcttcaagc
     7501 tgcagctcga aggcggcggc ggcgaactgc acttcgcgct gccctactcg atgatcgagc
     7561 cgatccggca cctgctggat gcgcgcccgc cggccgagcg cagcgagggc gacgggcgct
     7621 ggcagaaatc gctgcgcgac gaaatgcggc tcgcgccggt cgaggtgagc tgcaagctct
     7681 gcgaaatcga gctgagcgtg gcggagctgc tgcggctcag gcccggggac gtcctgccca
     7741 tggacatgcc ggagcgggtg acgctctacg cggagggcat cccggcattc cacggtcgat
     7801 tcggcgcctc ccaggggcgg catgcagtac aggtcatcga accggcccgg cgcgagcacg
     7861 ccggcgccga catcaagcga gaacttagcc aggagcgcgg aagatgagtg agcgacagac
     7921 acaggcgagc acggaagaga cgggcgcggc gcgggccggc gccgcggcgg ctgccgcgga
     7981 cggcggcgcc ggccagggcc cgaggaccgc caaggccggt acgccggtgc cgccgaagcc
     8041 gctgaaggac gagggcgggg cggccggcga ggacgtcaac ctcgaggtcg tgctggacat
     8101 cccggtcacc ctgtcgatgg agatcggccg ggcgaagctg ccgatccgca acctgctgca
     8161 gctcaaccag ggctcggtgg tcgagctcga gcgcatggtc ggagagccga tggacgtgct
     8221 ggtcaacggc acgctcatcg cccacggcga ggtggtggtc gtcaacgaga agtacggcat
     8281 tcgcctcacc gacgtcatca gcccggccga gcgcgtgcgc aagctgcgct gagcacccca
     8341 tgaagcgctt gctgaccctg ctcgccgtac tgcttctgcc gctggccgcc gccggcgccg
     8401 agccggctgc cgcgcccggt ttcgccggcg ccggccaggt cggcaagatg atcgccggcc
     8461 tgttgctgat gatcgtcatc atcatcggcc tggctgcgct gctgaagcgc gtcaacggcc
     8521 tgcagcgcag cctgcccggc gccatccggg tggtttcggt cacctccatc ggcgcgcgcg
     8581 agcggctgat gctggtcgag gtcggcggac ggcagctgtt ggtgggcgcg gcgcccggac
     8641 gggtgcagac cctgctggtg ctggaccagc cggtcgccat gccgcagacc gcggcgggcc
     8701 gccaggcaag cggattcgcg gagcgcctgc gggatgcgct gggcgcccct gccaaggagg
     8761 cgcgctcgtg acgcgacgcc tcggatggct cctgctgttg ctggcggcgt tgctggcctg
     8821 gccgttgtcg gcgggcgccg agaccgccgg cggcaatgcc agcggcctcg agctgctctc
     8881 ggtgagcacc gccgaggacg gcagccagca gtactccgtc acgttgcagg tcctgctgct
     8941 gatgacgctg ctgagcctgc tgccggcggc gctcatcatg atgacctcgt tcacgcgcat
     9001 catcgtcgtg ctggcgatcc tccgccaggg catgggcacc cagcagacgc ccaacaacca
     9061 gatcctcatc ggcctggcgc tgtttctcag catgttcgtg atggcgcccg tgctggagcg
     9121 catctacgag gatgccgcca ggccctacct cgaggaaacc ctcaacgcgc cggccgcgct
     9181 gcaggccgcc agcggaccga ttcgcgagtt catgctcagc caggtccgca cgccggacct
     9241 gcagctgttc gccgggctcg gcggccacga gggcttcgag gcgccggagg acgtgccgtt
     9301 ctctgtgctc gtgccggcct tcgtcaccag cgaactcaag accgccttcc agatcggctt
     9361 cctgctgctg gtgccgttcc tgatcatcga cctggtggtg gccagcgtgc tgatgtccat
     9421 gggcatggtg atgctctcgc cgctgatcat ctcgctgccg ttcaagatca tgctgttcgt
     9481 gctggtggac ggctggtcgc tggtcatgag cacgctggcc gccagcttca tggtgagctg
     9541 acgcatgtcc cccgaactgg tcatggaagt tggacgcaac gcgctggcgg tcacggtgat
     9601 gctggccgcg ccgatgctgg tcgtcgccct ggcgctcggc ctgttcatcg gcatgatcca
     9661 ggcggccacc caggtccagg aaatgacgct gtccttcatt cccaagctgc tcggactggt
     9721 cgccgtgctg gccatcggcg ggcactggat gctcgggctg atcgtcgact acacccgcga
     9781 gctcatcacg gccatccccg gcctgctgct cggctgagga accgccccat ggagttcacc
     9841 cagctgcagc tcgacacctg gatttccagc ctcgtgctgt cgttcacccg catcgccgcc
     9901 ctgctgatcg cggcgccgct gttcggcggg cgcagcctgc cgatgcgggt gcgcgtgtcc
     9961 ttcgcgctgg tgctgtccat gatggtgctg ccgatcctgc cgccggcgcc gccgctcgag
    10021 ctgttgagct ttccgatggt gctggcggtc ggtcgcgagc tgctgatcgg cgccatggcc
    10081 ggcttcatcc tgcagctggt gtttgccgcg ctggccattg ccggcgaagt gatcgccctg
    10141 ggcatggggc tagggttcgc atcgctggtg gacccggaca agggcgtgca ggtgccggtg
    10201 gtcgggcagt tgctcgtgat catggcgacg ctgctgttcc tggccatgga cggacacctg
    10261 gcgctggtca gcttcatggt gctgagcttc accggcgtcc cgatcggcgg cggcatcagc
    10321 ttcgagaatt tcgccgccat cgccggttgg ggcagcgtga tgttcggcca tgcggtgatg
    10381 atcgcgctgc cggccgtcgg cgcgctgctg ctcgccaacc tggccatggg cgtggtcaca
    10441 cgcgccgccc cccagctgaa catcttcgcg gtcggcttcc cgatgacgct gatgctcggc
    10501 ttcgtgctgc tgtacgccac cgtcggcacg atcggcggtc gcttcgcgga gctgctggcg
    10561 gagggcttcg gcatgatcgg ctggctgttg ggaggctgag gcgatggccg agcaggacaa
    10621 gagccaggaa cgcaccgaac aggcgacacc caagcgccgc caggatgcga agaagaaggg
    10681 ccaggcgccc cgctcccgcg agctgaacac caccgcggtg ctgctggccg gcgccggcac
    10741 gctggtgctg ttcggcggcg ccatcggcga aggcatcgcg gccatcatga tcgacagcct
    10801 gagcccgtcg cgcgagctgg ccttcgacgc gccgctcctg ccgacggtct tcggccagtc
    10861 ggcgatggtc gcgctggtgc tgctgctgcc gctgttcgcc gcggtgctga tcgccgcact
    10921 ggcgggcccg gcgatgctgg gcggcctcgt gttcagcgcg caggccctgc agccgaaact
    10981 cgagaagctc aacccgatcg ccgggctgaa acgcattttc tccctgaacg gactgatgga
    11041 attcgccaag acgctggcca agttcgtcgt cgtcaccggc gccggcctgc tggtgctccg
    11101 ccacatcgct ccggacctgc tgatggccgg cgaggccagt atcgagaccg cggtgcccgg
    11161 ccttgccgct gccgcggcct gggtgctggt ggtgcttgcc tccgtgatgc tgctgatcgc
    11221 ggcggtggac gtgccgttcc agctgtggaa ccacagcaag cagctgcgca tgacgcgcca
    11281 ggaagtgcgc gacgagatga aggagacgga gggccggccg gaggtcaagg gccgcatccg
    11341 ccgcgtgcag atggaaatga cccagcgccg catgatgatg gcggtgcccg aggccgacgt
    11401 ggtggtcacc aacccgacgc actactcggt ggcgctcaag tacgacccgg acggcatggc
    11461 cgcaccgcgc gtcgtcgcca agggcgccga ccagatcgct ctcaagatcc gcgaagtcgc
    11521 cgccgccagc ggcgtgccgc gcgtcgaggc gccgccgctg gctcgcgccc tgtacttcac
    11581 gacccggctg gacgaggaaa tcccggccga actgttcctc gcggtcgccc gcgtgctggc
    11641 ctgggtatac cagctcaagg ccgccgccgg cgccgcgccg ccgccgcagc ccgaagggct
    11701 cgacgttcct gaccacctga cccgccgcca gcccggcagg agaatctgac gatgggcaat
    11761 ctcgcaaccg gtttcggcac catcctgcgt accgggctcg gcacgccgct gctgctggtc
    11821 gccctgctgg tgatgatcgt cctgccgctg ccgcccatcg cgctggacgt gttcttcacc
    11881 ttcaacatcg cgctcgcgct ggtggtcatc ctgatgaccg tgtacgcgcc gcggccgctg
    11941 gaattctcgg tgttcccgac ggtgctgctg ctggccacgc tgctgcggct ggcgctgaac
    12001 gtcgcgtcga cgcgcgtggt gctgatcgac ggccacaccg gcaccgacgc ggccggcaag
    12061 gtcatcgagg ccttcggcga tttcgtggtc ggcggcaact acgcggtcgg cctggtggtg
    12121 ttcgccatcc tggtcatcat caacttcgtg gtggtcacca agggcgccgg gcgcgtgtcc
    12181 gaggtcagcg cccgtttcac cctcgacgcc ctgccgggca agcagatggc catcgacgcc
    12241 gacctgaact caggcctgat cacccaggac gaggcccgcg cgcggcgcgc cgagacggcc
    12301 gcggaggccg atttctacgg ctcgatggac ggtgccagca agttcgtgcg cggcgacgcg
    12361 gtggccggca tcctgatcct gttcatcaac atcatcggcg gtctgatcat cggcacggtg
    12421 acgcacgaca tggcgctggc cgacgccagc cgcacttaca tcctgctgac catcggcgac
    12481 ggcctggtgg cgcagatccc cgcgctgctg ctgtcatcgg ccaccgcgat catcgtgacc
    12541 cgcgtgtccg ctgccggcga catgggccgc gacgtggtcc ggcagttgtt cggcagcgcg
    12601 cgcgcgatcg gcgtcgcggc cgccatcatc ggcatgctcg gcctggtgcc gggcatgccc
    12661 aacctggtgt tcctcggcct cgctgcggcc ctcgggaccg cggcgtggat gctgcacaag
    12721 cgctcccggc agaccgaagt cgaagtcctg cccgaggagt ccgccgagcc ggccgcgccg
    12781 cgcgaactgg cctgggacga cgtcagccag gtcgacccga tcggcatgga ggtcggctat
    12841 cggctgatcc cgctggtgga ccgcgcgcag ggcggggtgc ttttggaacg catgaagggc
    12901 gtgcgccgca agctttccga ggagctcggc ttcctggtgc cgccggtgca cgtgcgcgac
    12961 aatctcgatc tcggcccgaa cggctaccgg atcacgctca acggcgttcc ggcggcgcgc
    13021 ggcgaaattc acgccgaccg gcacctggcc atcaatccgg gccgcgtcta cggccagatc
    13081 gcgggcaccg agaccactga tccggccttc gggctgcccg cagtgtgggt cgccgccgag
    13141 cagcgcgacc aggcgcagac cctcggctac acggtcgtgg acccggccac ggtggtcgcc
    13201 acgcacctca gccaggtgct gcgcgatcat gcccacgagc tgctcggcca cgacgacgcc
    13261 cagcagctgc tggaccggct ggccaagagc catcccaagc tggtggagaa cctggtgccg
    13321 aaggtgctca gcctcggcgt ggtgctcagg gtcatgcaga acctgctggc cgagcagatc
    13381 ccgatccgcg acatgcgcac catcgccgaa actttggcgg aacacggcgc ccggagtcag
    13441 gatcccgtcg ccctcacggc cgccgtgcgc actgcgctgg cccgattcat cgtgcaggga
    13501 atcactgatt ctcatgaaga tttgccgctg attgcgctgg atcccgggtt ggaacaagtc
    13561 ttgcaacgga gtctgcaggc aacgggttcg gcgggtctcg gcatcgagcc ccggctcgcg
    13621 gaaaggctcc agcgcggagc cagcgaggcg gcgcagcgac aggagatcgc cggcgagccc
    13681 gcggtgctgg tcacttcggc cgaactgcgg ccctggctgg cgcggtggct gaggccggtg
    13741 gtccgggggc tgcacgtact ggcctacacg gagataccgg acagccggcc ggtgaaggtg
    13801 gtagcgactc tcggcgcggc cgaggcacaa gcggcgaccg cgtaggcggc atgaaaaggg
    13861 attgaggacg actatgaaga tcaagcgatt tttcgcgcca gacatgcgcc aggcgatccg
    13921 catggtgcgc gacgagcagg gtcccgacgc ggtgattctc tcgacccgca acaccggcgg
    13981 cggagtggaa atcgtcgccg cgatcgacta cgacgcggag ctcgtcaacg gcatgctcgg
    14041 cgagccgcag acagagcagc gcgatgccca ctccgcgccg ccgcatccgg cggaagcggc
    14101 ggcccgggca gaggtcctgc ccgagcccgc cgcgccggcc gtcccgccgg ccacggaggc
    14161 gctgccggag ccggagatcg cggaggttcc cgagttgctg aagcgcagtg cgccgtcgcg
    14221 gacgccgcgc gtcgagtgga cccaggaccc gacgctggcg gcgatgcagg acgagctgaa
    14281 gtcgctgcgc gccatcctgc aggaccaggt gacgcgcctg gccacggccg actacgaccg
    14341 gcgcgagccg gtgcgcgccg agattgcacg ccggctgatg aaggcaggtt tcgaagaggc
    14401 ggtggcccgc ggcgtggccg ccgggaccac cagcctcgcc accccggccg aggcctggcg
    14461 cgaagcgcag ttcgggctcg cccagcgcct gccggtggtc gcgacggatc ccgtcgagca
    14521 gggccacgtc atcgccctga tcggcgcgac cggcgtgggc aagacgacta ccgccgccaa
    14581 gctggcagcg cgcgcctgcc tgcgctacgg cagcaagtcc gtggccctga tcagcaccga
    14641 cgatttccgc gtcggcgccc agcgccagct tgcctccttc gggctgctgc tcggcctgcc
    14701 ggtgcggcag gcgcgctcca ccgccgagct ggccgatcaa ctggccgagt tcgacaatcg
    14761 ccgcctggtc atcatcgaca cggccggcat gggccagcgg gacgtccggc tgatcgagga
    14821 aacgcgcaag ctcagccagg tcgccgagct gcagagctac ctggtgatgt ccgcgaacct
    14881 gcaacgcgag gtcatggacg aggtcgtgga cgctttcggc cgcgaccagc tggccggctg
    14941 catcctgacc aagctcgacg aggcggcgcg gctcgggccg gcgctctcca ccctgctgac
    15001 tcaccagctg ccggtggcgt tcgtcagcga aggccagcgc gtgccggagg acctccgccc
    15061 ggcccgcgcc cgcgacctgg tgagccgcgc gctggcctcg cccgcgcgcg ctgcccggcc
    15121 gggaccgtcc ccgacagccg ctgccggcac cgtgcaggag gttcgccatg catcagcctg
    15181 agctcgcgcg ccaggccaac ggcctcgcct ctttccgtca gccccgcccg gtccgcgtgc
    15241 tggcggtggc cagcggcaag ggcggggtcg gcaagaccaa cgtgtccgtc aacctcggcg
    15301 tcgccttcgc cggcctcggc cgacgcaccc tgctgctgga cgccgacctg gggctggcca
    15361 acgtggacgt gctgctcggc ctgcaggtcc cttacaacct ctcgcacctg ctcggcggcg
    15421 agcgcgagct cgacgagatc atcgtctcgg gcccggcggg cctgcgcatc gtgccggcgt
    15481 cctccggcgt cgcgcggctg gccaacctga gcccggccga gcatgccggg atcatcggcg
    15541 ctttcagcag cctggccgaa ccggtggacg tgctgatcgt cgacgtcgcg gccggtatca
    15601 gcccggcggt gctgcagttc tgcgtggccg cgcaggaagt catggtcgtc gtttgcgacg
    15661 agccttcctc gctgaccgac gcctacgcgc tggtcaaggt gctggcacgc gaccacggcg
    15721 tgcgccgttt ccacaccatc accaaccgcg tggactcgcc gcaccacggt cgccggctgc
    15781 accacaagct gcagagcgtc tgcgaccgct tcctcgacat tcccctccag catctcggcg
    15841 ccgtgccgga ggatcccctg ttgcgacggg cggtgcagat gcaggcgccc gtggtcactg
    15901 catatccgtc cagccccgcg gcacgggcat tcaaggatct ggcccggcat gccgataccc
    15961 tccctgaacc ccgcgggatg agcggcagca tcgagttctt cctggaacga cttgccggcc
    16021 aggatccccg ggaggacggc ctcgaggcgg tgggctgaac atgtacgccg cagcgagaga
    16081 gttcgatggc gaacacgtgg tcgagcggta tctcggcctg gtcaaacgga tcgcgtgcca
    16141 cctgctgggc aggttgcccg ccaacgtgca actggaggac ctggttcagt ctggaacgat
    16201 cggcttgctg gaggcggccc ggcactacga cgccgggcag ggcgccagct tcgagaccta
    16261 cgcggggata cgcattcgcg gcgcgatgct cgacgaggtg cggcgcaacg actggacgcc
    16321 gcgctccgtg caccgcaacg cgcgccgggt gtcggcggcg atccgcgcgg tcgagagcga
    16381 gaccggccgc gaggcgcagc cgcgcgagat cgcggcggtc ctcgaagtgg gcctcgacga
    16441 gtatcacgcg atggcccggg acacggtggc cgcgcggctg ttcagcctga acgacctcac
    16501 ggaggaggac ggcagtttcg aggacatgct ccccggcgag gaagccggtc cggcggagga
    16561 attcgatcgc gaggccttcc ggcgggacct cgcggtggcg atcagcgggt tgccggagcg
    16621 cgaacagctg gtgatgtcgc tgtattacga gcaggagatg aacctgcggg aaatcggtgc
    16681 ggtgctcgga gtgacggaat cgcgcgtctg ccagctgcac ggccaggcgc tggcgcggat
    16741 ccgggcgcgg ctcaggaact ggacggacga tgagggcatg gactaggcgc ggcgcggccg
    16801 caacggagga cgcgtgagcg cgacgggaca gacggtcggt cagggagtcg gcggacggca
    16861 ggccacgggc gcgcctgcgc ctgcgggccg tggcggcctg gacgtcctca gcgtcgccgg
    16921 cctggcgctg ggtgtcgtcg ccatcctggg cggcagcatc ctcaagggca gcggtctcgc
    16981 ggcgctctgg aatggcgccg ctttcgtcat cgtcatcgtg ggtaccctcg ccgcgaccct
    17041 gctgcaggtg cgcctcgaaa ccttcaagca tgccatgaag atgtttcgct ggatcatcca
    17101 gccgccggcc aacgaccccg cgcaggccat caccaagatc gtcagctgga gccaggtggc
    17161 gcgcaaggag ggcctgctcg gactcgagag caccgcggaa gaggagcccg acgagttcct
    17221 gcagaaaggc ctgcagttgc tggtggacgg cagcgagccc gaggcgctgc gcggcatcct
    17281 ggaggtcgac atctcgaccc gcgaggaatt cgacaaccgc ggcgccaagg tattcgagag
    17341 catgggcatc tacgcgccca cgctcggcat catcggcgcg gtccttggcc tgatcgcggt
    17401 gatgggc
//
LOCUS       DAOMPG010000019        17335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1457_length_17335_cov_3.903762, whole genome shotgun sequence.
ACCESSION   DAOMPG010000019 DAOMPG010000000
VERSION     DAOMPG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_17335_cov_3.903762"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..644
                     /locus_tag="PLS34_01880"
     CDS             243..644
                     /locus_tag="PLS34_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86254.1"
                     /translation="MIALLLRALLVGILIAAIETVHGILRVRWLNRRIGDKRARRVTV
                     VSGSLLVLFTSWVTVPWLDIRSQAEAWITGSIWVALLLAYDLGLGRWYFGFSWQRLFA
                     DFDPRQGGMLGLGMLVLLAAPWLACVARGLL"
     gene            complement(667..1746)
                     /gene="pheA"
                     /locus_tag="PLS34_01885"
     CDS             complement(667..1746)
                     /gene="pheA"
                     /locus_tag="PLS34_01885"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRP86255.1"
                     /translation="MDLTPIRTQIDSLDRQIVELLNQRLALAAEIGKVKRSQGGRIYV
                     AEREDAVFRKISELNRGPIKNDALQAIYREVMSAAIALEKPLLIAFLGPEATNTHAAA
                     MKKFGASVDYHAMASISDIFTAVEKGEADYAVIPIENSTEGSVRETLDCFVESDLKIV
                     SQVYLEITHALISNSSLERIEKVYSKDQAIAQCRHWLQRHLPHAQLIDAASTSRAVQI
                     AKEEPGSAAIASALAAQYHGGTVVSKNIQDKADNTTRFFVLGKKPSGHVGNGRDMTSL
                     LISPDDEASGRSGTLLKMLMPLAERGINLSKIESRPSKRRPWDYYFFIDVSGHYDDPE
                     LKQALAELKVFCPLVKWLGSYPSVA"
     gene            complement(1746..2726)
                     /gene="scpB"
                     /locus_tag="PLS34_01890"
     CDS             complement(1746..2726)
                     /gene="scpB"
                     /locus_tag="PLS34_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRP86256.1"
                     /translation="MAFNLQKVLKALLFSSSQPLAIKDIQAVFSRFHEQATLPLAPEE
                     PSDDTKSAEPSPIPDGEGASSDASAASPVEDSPTAHEAAVEPVAASSDTEDAELYRDV
                     PSLVTATQIREAMAALIEAIRQENGVFLLVEGANGYRLVTHPRYARWIRILRDEPPPV
                     KLTQSALETLAIVAYRQPVTRTEIETIRGVSAEAGISKLLERDMIYIVGRADLPGRPL
                     QYGTTDKFLDFVGVKSLVELPSSDVLSPRQIDEWLKSAINPKPLTDAEMGLPLEEGEG
                     PSPNRESVTLEHGGHGGDNAQTESAPVENPGLSPSLAEADEGGQKESHSA"
     gene            2840..4531
                     /gene="ilvD"
                     /locus_tag="PLS34_01895"
     CDS             2840..4531
                     /gene="ilvD"
                     /locus_tag="PLS34_01895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRP86257.1"
                     /translation="MSTTPDSKRPYSSIVLDGPDRAPNRSMLRAVGFKDDDFKKPVVG
                     IASTWSMVTPCNMHIDQLAREAEAGANAAGGKAIIFGTITVSDAISMGTSGMRYSLVS
                     REVIADSIETVTGAEGFDALVTIGGCDKNMPGCVMAMTRLNRPSVFVYGGTILPGIHP
                     DSKKECDIVTVFEAVGQHAAHKLDDKGLETVEGCSIPGPGSCGGMYTANTMASAIEAL
                     GLSLPNSSAQEAVGAAKAEDCRRAGAAVLELLARGIRPSHILTREAFENAITVTIALG
                     GSTNAVLHLLAIADAAGVALELDDFNRIGARVPVLADLKPSGKYLMSELIEIGGIRPL
                     MKTLLDAGLLHGGCLTVSGRTLAEDLAGVAPYPPGQDVVRPLDRPVKADSHLVVLKGN
                     LAPAGAVAKISGKEGERFVGRARVFDGEQDALAAILAGRVVAGDVVVIRYLGPKGAPG
                     MPEMLSPTGAIIGRGLGDSVALITDGRFSGGSHGFVVGHVAPEAAVGGPIAAIADGDE
                     IEIDARRREITLRVGPAELARRLEDWRAPAGEKPGTVLEKYGRLVSCASKGAVLS"
     gene            4624..5172
                     /locus_tag="PLS34_01900"
     CDS             4624..5172
                     /locus_tag="PLS34_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP86258.1"
                     /translation="MFEFSKKGTPDETAEASSPDRSRAAPDSPYRATASGSSGARRDA
                     AVIGPSIQLDGDLRGQEDLLIEGEVSGTVQLRENSLTVGAQGKVKADVYAKEIHVEGT
                     VEGDLFGAERVVIRKSAQVRGNITSPRVAIEEGARFKGAIEMDSTAVEAALGKTRGAA
                     GAARAKPVEPAAAATTRSGAAS"
     gene            5183..5749
                     /locus_tag="PLS34_01905"
     CDS             5183..5749
                     /locus_tag="PLS34_01905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP86259.1"
                     /translation="MPELNAPLFRGLLQGMEDDARRVVLDLGPARSATVATFGRFRCR
                     LEITDLGAAVDTLNATEETAELRAALRALLPPSRPEATDLVLCWDLLNYLEPPVLKAL
                     MDEIQSRLRPGSLVHALIAYSTPRMPARPQHITPTPGYGLEIVPLDGAERAARRYTPD
                     ELGRLMGGYVVERAMLLKNGMQEFLFRH"
     gene            complement(5777..6238)
                     /locus_tag="PLS34_01910"
     CDS             complement(5777..6238)
                     /locus_tag="PLS34_01910"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRP86260.1"
                     /translation="MRALELKVPPVAQFLVAAGIMWLLAWLVPAASVAFPGQLLAASV
                     VLLVAGLVGMAGVRAFARAETTVNPLRPGSATRLVAHGIYRRSRNPMYLALLLALLAW
                     AVLLGNGLALLVPPVFLLAMNRLQIRPEERALAIVFGEEFLAYQRQVRRWL"
     gene            complement(6243..6728)
                     /locus_tag="PLS34_01915"
     CDS             complement(6243..6728)
                     /locus_tag="PLS34_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRP86261.1"
                     /translation="MKIHAHLLVAALAVALGCIASAAHAEEDGAVLERGRYLARVAGC
                     NDCHTTGYPQSGGMVPESEWLKGDALGWRGPWGTTYPGNLRLLAARITEQQWIELART
                     ARYRPPMPWFALRDMSDEDLVALYRLLRHLGPAGEPAPAWVPPDQEPQGPFVQFPAPP
                     G"
     gene            complement(6829..7674)
                     /gene="rhlP"
                     /locus_tag="PLS34_01920"
     CDS             complement(6829..7674)
                     /gene="rhlP"
                     /locus_tag="PLS34_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373625.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HRP86262.1"
                     /translation="MLEPSTQLRLGVVFLLGVALLATGCATTQSRQTTSIVDFLYPDA
                     KEPVADQGIPVLNLPMRVGVAFVPEAHGRGSTVLSEARKRQLLEEVAGHFRDREFVRA
                     IEIIPSAYLRPQGGFQNLEQLRTMHGVDVVALVSYDQAQFTDEGLSSLAYWTLIGAYI
                     VRGEKNSTHTMVDAVIIDIPSRKMLFRAPGTSFVAGTATPVNLSEQLRLDAGRGFEAA
                     VADMIGNLDQELVVFQDRVRSNPDEYRVVRTPDYKGGAGSPGLFDALLLLVLGGRMLR
                     RRRGG"
     gene            complement(7793..9145)
                     /locus_tag="PLS34_01925"
     CDS             complement(7793..9145)
                     /locus_tag="PLS34_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP86263.1"
                     /translation="MNGAYLLLNFNALFALREYFLGRRVDVLPRAYSDYEMPITIIVP
                     AYNEQATIVASVRALLQLRYPEYEIVVINDGSRDGTLQALIDAFRLQPFPEAYRQRLA
                     TAEVRRVYRSTLHDNVRVIDKANGGKADALNAGINASRYPLFCCVDADSILHPDSLYR
                     VLQPFLDDPRTVACGGTVRIVNGSTVRDGFVERVRLPRSPLALLQIVEYLRAFLFGRL
                     GWSPMNALLIISGAFGLFRKEVVVAAGGYRTDTVGEDMELVVRLHRVLRAAGKPYRIT
                     FVPDPICWTEAPEDLRTLARQRIRWQRGLGESLAKNIGLMTQRRAGVVGRFAYPFNLL
                     FELFGPLLEVAGYLFIIIAALNGWMSLEASLVFLLVAVGLGVVLSTSALLLEELSFHV
                     YPGLRSTLVLFAVAILENFGYRQLNAVWRLVGLLRWLGGGQAKWGEMRRGGKWSQPRS
                     "
     gene            complement(9196..10269)
                     /locus_tag="PLS34_01930"
     CDS             complement(9196..10269)
                     /locus_tag="PLS34_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP86264.1"
                     /translation="MPGFATTSDLLLRVALLTGLGAVSIALLVFLGILWLRARLMRSQ
                     RRRAAFNARWRPVFAEVAIGGGDDSSGPALPALPSRDRRLFLVEWNILHDLLRGEPKL
                     RLNALAARLGIDRYARQLLRTGRLADRLLAAATLGHLRDSGAWNELLAMLGSENTLVS
                     LVAARALISIDPEAALPRVLPVIRDRDDWSTPRVADLFMEAGPEAVSAPLEAAIAACP
                     PEQVPKLIAVLPEVALPVSERIVSRLLRAPTDDRIKSICLRILGNPRDLPVVRDLAAH
                     PRWHVRMNVAVVLGRMGLPQDSDTLVAMLSDPEWWVRYRAAQALLAMPFVTLAEVART
                     RDAMGDEFARDILDHVMAERAAC"
     gene            complement(10244..11308)
                     /locus_tag="PLS34_01935"
     CDS             complement(10244..11308)
                     /locus_tag="PLS34_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HRP86265.1"
                     /translation="MPARVLVLLLGLALLPVGAAMPDDARLAELEAQLAEDPGRLEAR
                     FEYARALSWAERWDEALREYDRLLAAAPGNPDYLLGKAQVLAWSGRPAAALPLLEEAR
                     QVAPDYQAVIRLEAQVRERLAARPAESLEAGLGWQDLGSGLPDWRSQYIEYQRSHALG
                     YRVFGSVQREERFDASDVEGRAGWARPWNDDWSFVLEGSVAPGAEVLPRWSGRIRLHR
                     DIGDGWGVYGGVSQSGYAGDDLATATVGAERYTGPWHAALTVQASRLQRADTTWSGHA
                     RLDRYYASDSRIGLIAAAGRETESIGDGRFLTSSTLGLALLGLHRVAPHWSVSWELLV
                     HEQGDIYTREGFRAGLRYDF"
     gene            complement(11308..11736)
                     /locus_tag="PLS34_01940"
     CDS             complement(11308..11736)
                     /locus_tag="PLS34_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP86266.1"
                     /translation="MTRTEGAAPPLLQDSPRALVIGPDEHTAFLLEYMLQREGFVVAS
                     FPEGASAAEHIDREPPADVVVTDIVMPFVDGFELITRIRADERWRAVPLIVLSAHSAD
                     QEVIRALELGANDYVTKPFNAGVLLARIRRHMQTATGARA"
     gene            complement(11823..13697)
                     /locus_tag="PLS34_01945"
     CDS             complement(11823..13697)
                     /locus_tag="PLS34_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP86267.1"
                     /translation="MALLEVDKLNVRFHTPDGEVHAVRDLGFSLQRGETLGIVGESGS
                     GKSQSMMSLIGLLASNGRASGSARFDGAELIGMPVAALRRIRGRRIGMIFQDPMTSLN
                     PYMTVFEQMAQVLAHHEKLGRKQTRARCIQLLEKVHIPEAAKRVDMYPHEFSGGMRQR
                     VMIASAMLCRPDLLIADEPTTALDVTVQAQILELMREVRRDFGTSIILITHDLGVVAG
                     LCDRVLVMHGGEEKEAGPVDDIFYRPRHAYTRALLAAVPRLDRAEATRLASLDASATS
                     ADGTALQGAAAAAAAAPVVRREPDYAAPPLLVVDDLRVHFRLIPETTFARPRLLKAVD
                     GVALDLRPGETLGVVGESGCGKSTLARAVLRLVPATTGRVCLLGEDITAKDKKAMRIH
                     RRDMQVIFQDPLASLNPRMTVGGIIAEPLWTHFPAMPRREVRQKVEAMLGRVGLGPEH
                     VNRYPHEFSGGQCQRIGIARALVLQPKLVICDEPVSALDVSIQAQIVNLLMDLQAEMN
                     LSLVFIAHDLAVVRHISHRIMVMYLGRIAEIADRNALYEQPMHPYTQALIRAVPIPDP
                     QKERAKPYAPLQGDLPSPVDPPSGCAFRTRCPIAVERCAIETPALRRVGEHRVACHEA
                     "
     gene            complement(13697..14602)
                     /locus_tag="PLS34_01950"
     CDS             complement(13697..14602)
                     /locus_tag="PLS34_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP86268.1"
                     /translation="MSAVPQLLPPSGGSGIEPVKGRSLWIDAWQVLRRNRAAVVAGAL
                     ILTMAVLVIVGPWLSPWAYDHTDWANTSIGPDWESRHYFGTDALGRDLFVRTLYGGRI
                     SLLIGVVATLVSLLIGISWGATAGYLGGRVDHVMMRIVDILYAMPFMFFVILLMVFFG
                     RNIILIFVAIGAINWLDMARIVRGQTLSLKHKEFVEAAEAQGVSTFNIIRRHIVPNLL
                     GVVVVYVTLTIPQVILVESFLSFLGLGVQEPMTSWGALVNEGAQEMGTAPWMLVFPAV
                     FLALTLFCFNFVGDGLRDALDPKDR"
     gene            complement(14599..15522)
                     /gene="oppB"
                     /locus_tag="PLS34_01955"
     CDS             complement(14599..15522)
                     /gene="oppB"
                     /locus_tag="PLS34_01955"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HRP86269.1"
                     /translation="MLRYALTRFLGAIPTLLILITIAFFMIRAAPGGPFDAEKALPAE
                     IEANLRAAYHLDEPLIQQFGRYVWNLVRGDFGPSFQYKDYSVTELIAAGFPVSLRLGG
                     SAMLLALLIGVTAGTIAALRQNSRTDHVVMAVSMTGISIPNFVMAPLLILLFAVMLGW
                     LPAGGIGDGGLRHMLLPVISLALPQVAYISRLTRGSMIEVLRSNFVRTARAQGLPERT
                     VILRHALKPALLPVVSYLGPATAAVITGSVVIEQIFGVPGLGRYFVQGALNRDYTLVM
                     GVVVFYGALIILFNLLVDLVYAWLDPKVKYA"
     gene            complement(15569..17215)
                     /locus_tag="PLS34_01960"
     CDS             complement(15569..17215)
                     /locus_tag="PLS34_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86270.1"
                     /translation="MTRCRWIAPLLLMMSLAAGCGDPASRAERTAPVRIGGESGAELA
                     ERQVLHRGNGAEPQSLDPHKSEGVPESNLQRDLYEGLTNDAPDGIVPGAARAWEISED
                     GLTYLFHMREDGRWSNGDPVTAHDFEYGLRRSVDPQTLSNYSIMLRPILNAEQIIAGE
                     LPPEALGVRAIDDLTLEIRLAGPTPYFLGVLTHSASYPVHRATVERYGDRWARPGRLV
                     SNGAFRLRDWVMQSHIVLERNEHYWDAANVTLEEVWYYPIENQSTELQRYRADALDYT
                     YDLPFRQLAWIRANLPEELQIHEYLGIYYFGLNVTRPPFKDNPELRRALYLAIDRDII
                     TSQVTTAGEIPAYGWVPHAANYHQYVPEWASWTQEERNAEARRLYEAAGYSRDRPLRL
                     QILYNTHENHRTISVAVSAMWNEVLGIQTELVNEEWKVFLETRRTKQTTQVFRAGWIG
                     DYNDANTFAEMMHSQSGLNDSGWNNPRYDELLALAAQENDMARRAEYLYEAERVLIHD
                     VPLIPIYFYVTKRLVKPWVGNYRPHIQDIVYSKDLKILKH"
BASE COUNT         2916 a         5910 c         5810 g         2699 t
ORIGIN      
        1 actcgagggg ataaggaccc aaccgcttcc caagtcgcgt atcgtcacgc aggcccgccg
       61 cacgtcgtga tacgggcagg atgcgcatgg caagatcgtc aatgcgggcg cgttcgtctg
      121 catccgctgt ggtcacctgc tccggcggtg tcgccagcac gtgtcggatc acttgtccgt
      181 cggccacagc aatccagacg gatcaccttg cacttcgtcc gaatgcgccg aatctcccgt
      241 acatgattgc gctgctgctt cgggcgctgc tggtcggcat tctgattgcc gcaattgaaa
      301 cggtgcacgg tatactgcgc gtgcgctggc tgaaccggcg tatcggagac aaacgagcga
      361 gacgggtcac cgtggtttcg ggatccctcc tcgtcttgtt cacttcctgg gtgacggtgc
      421 cctggcttga catacgcagt caggcagagg catggatcac aggctcgatc tgggtggcgc
      481 tgctgctggc ctatgacctg ggactcggcc gctggtactt tggtttttcg tggcagcgtc
      541 tgttcgcgga tttcgatccg cgccagggag gcatgctcgg cctgggaatg ctcgtactgt
      601 tggcagcgcc ctggctggcg tgcgttgcac gcgggctgct ttgagtgtcc tgaagaggtc
      661 agcccctcag gctaccgagg ggtagcttcc gagccacttc accaaggggc agaatacctt
      721 caattccgca agggcctgct tgagttcggg atcgtcgtag tggcccgaga cgtcgatgaa
      781 gaagtagtag tcccacggac gcctcttgct cgggcgcgat tcgatcttcg acaggttgat
      841 gccgcgctct gcgagcggca tgagcatctt gagcagggtg ccggagcgtc ccgatgcctc
      901 atcgtccgga gaaatgagga ggctggtcat gtcacgtccg ttgccgacat gccccgaggg
      961 tttcttgccg agcacaaaaa agcgcgtcgt gttgtcggcc ttgtcctgga tgtttttcga
     1021 caccacggta cctccgtgat actgcgcggc gagtgcgctc gcaatggcag cgctgccagg
     1081 ctcctccttt gcaatctgca ccgcgcgcga cgtgctggcc gcgtcgatca attgggcatg
     1141 cggcaggtgc cgttgcagcc aatggcgaca ctgcgcgatt gcctggtcct tggaatacac
     1201 cttctcaatc cgctccaggc tcgagttcga aatcagcgca tgcgtgattt ccaggtagac
     1261 ctgcgagacg atcttcagat cgctttccac aaaacaatcc agcgtctccc gcacgctgcc
     1321 ctccgtcgaa ttctcaatcg gaatcacggc gtagtccgcc tcaccttttt ccaccgcggt
     1381 aaagatgtcg ctgatcgaag ccatcgcgtg gtagtccacg ctcgctccaa acttcttcat
     1441 cgccgccgca tgcgtgttgg tggcttccgg tccgagaaac gcgatgagca gcggtttctc
     1501 cagcgcgatc gccgccgaca tgacctcccg ataaatcgcc tgcagcgcgt cgttcttgat
     1561 cggcccgcgg ttcaattcgc tgatcttccg gaataccgca tcctcgcgct ccgcaacgta
     1621 gatccgcccg ccctggctgc gcttgacctt gccgatctcc gccgcgagcg ccaggcgctg
     1681 attcagcagt tcaacgatct gccgatcgag gctgtctatc tgggtgcgaa tgggtgtgag
     1741 gtccatcagg ctgaatggga ttccttctga ccgccttcgt ccgcttctgc aagcgagggt
     1801 gacaaaccgg gattttcgac cggagccgac tccgtctgcg cattatcccc gccatggccg
     1861 ccgtgctcga gggtcaccga ttcacggttg ggcgaagggc cctccccttc ctcgagcggc
     1921 agacccattt cggcgtccgt aagcggcttg ggattgatcg cgctcttcaa ccattcatcg
     1981 atctgccggg gggacaacac gtcggaggag ggcagctcca ccaaagattt tacgcccaca
     2041 aaatcgagaa acttgtctgt cgtcccgtac tgcaagggcc gacccggcaa atccgcacgg
     2101 ccaacgatgt agatcatgtc gcgttcgagc agtttgctga tgcccgcctc cgcggaaacc
     2161 ccgcggattg tctcgatctc ggtgcgggtg accggttggc gataggcgac aatcgccagc
     2221 gtctccagtg ctgattgcgt cagtttcacg ggaggtggct catcgcgcag gatgcgaatc
     2281 caccgcgcat agcggggatg cgttaccagg cggtacccgt ttgccccctc aacgagcaga
     2341 aaaaccccgt tctcctgccg gatagcttca atgagtgcag ccatcgcctc tcgaatctgc
     2401 gtcgctgtca ccaacgatgg aacgtcccgg taaagctcag catcctccgt atcggaggaa
     2461 gcggccacgg gttcgaccgc ggcttcatgg gctgtgggag aatcctcaac agggctggca
     2521 gcgctggcgt ccgaggatgc tccctctcca tctggaatcg gcgagggctc agccgatttt
     2581 gtgtcatccg agggctcttc aggagccagc ggcagggttg cctgttcgtg gaaacgggag
     2641 aacacagcct ggatgtcttt gatggcaaga ggctggctcg aggaaaagag cagcgccttg
     2701 aggactttct ggagattgaa cgccatgcac ctaaagcggc tttgatgcgg ctctccctaa
     2761 cgggaagcaa cctcgaaaga gttggaaaac accacttgcc cgcaacggcg acgtctggtt
     2821 tcattggcac gatttgccca tgagcaccac tcccgactcg aaacgcccct attcctccat
     2881 tgtcctggac ggcccggacc gcgcgccgaa ccgttcgatg cttcgcgccg tcggcttcaa
     2941 ggacgacgat ttcaagaagc ctgtcgtcgg catcgcctcc acctggagca tggtcacccc
     3001 gtgcaacatg cacatcgacc agcttgcccg cgaggccgag gccggtgcca atgcggccgg
     3061 gggaaaggcg atcattttcg ggacaatcac cgtctccgac gccatttcca tgggcacctc
     3121 gggcatgcgc tattcactcg tttcccgcga ggtcatcgcc gactccattg aaacggttac
     3181 cggtgcggag ggtttcgacg cccttgtcac catcggcgga tgcgacaaga acatgccggg
     3241 ctgcgtcatg gccatgacgc gcctgaaccg tccgagcgtc tttgtctacg gcggcaccat
     3301 tctgccgggc atccatccgg attcgaagaa ggaatgcgac atcgtcaccg tcttcgaggc
     3361 cgtcgggcag catgccgccc acaagctcga cgacaaggga ctggagacag tcgaaggctg
     3421 ctccattcca ggccccggtt catgcggtgg catgtacacg gccaacacca tggcctccgc
     3481 catcgaggcg ctcggcctga gcctgcccaa cagctcggcc caggaggcgg tcggcgccgc
     3541 caaggccgag gattgccggc gcgccggggc ggccgtcctg gagctactgg cccgcggcat
     3601 ccggccgtcg cacatcctga cgcgcgaggc cttcgagaac gccatcacgg tgaccatcgc
     3661 gctgggcggc tccaccaacg ccgtgctgca cctgctggcc atcgcggacg ccgccggggt
     3721 ggcgctcgag ctcgacgact tcaaccgcat cggcgcccgc gtgccggtgc tggcggatct
     3781 caagccgagc ggcaagtacc tgatgtcgga gctgatcgag atcggcggca tccggccgct
     3841 gatgaagacg ctgctcgacg cgggactgtt gcacggcggc tgcctgaccg tgagcggccg
     3901 gaccctcgcc gaggacctgg cgggcgtggc gccctatccg ccgggacagg acgtggtgcg
     3961 accgctggac cgccccgtca aggccgacag ccacctggtg gtcctgaagg gcaatctcgc
     4021 gcccgccggc gccgtggcca agatctccgg caaggaaggc gagcgcttcg tcggccgcgc
     4081 gcgggtgttc gacggggagc aggacgcgct ggccgccatc ctggccgggc gcgtcgtggc
     4141 cggcgacgtg gtggtgatcc gctacctggg tccgaagggc gcgcccggca tgccggagat
     4201 gctctccccc accggcgcga tcatcggccg cggcctgggc gactcggtgg ccctgatcac
     4261 cgacggccgc ttctccggcg gcagccacgg tttcgtcgtc ggccacgtgg cgccggaggc
     4321 cgcggtcggg gggccgatcg cggcgatcgc ggacggcgac gaaatcgaga tcgacgcccg
     4381 ccgccgcgag atcaccctgc gcgtcggccc ggccgagctg gccaggcgtc tcgaggactg
     4441 gcgcgcgccg gcgggcgaga agcccggcac cgtgctggag aaatacggcc ggctggtgag
     4501 ctgcgcatcg aagggcgcgg tgctgagttg atgttcggcg gcggaagtgc ctatgcttgc
     4561 cgggcaagtc tctgggggga gcaacacgac tgacgcggtg tcgaggcacc cgggagaaac
     4621 accatgttcg agttcagcaa gaaaggcacg ccggacgaga ccgccgaggc gtcgtcgccc
     4681 gaccggtcgc gcgcggcacc tgattcgccc taccgggcca cggcgagcgg gtcgtccggc
     4741 gcccgccgcg acgccgcggt cattgggccc tccatccagc tggacggcga cctgcgcggg
     4801 caggaggacc tgctgatcga gggcgaagtc agcggcacgg tccagctcag ggagaacagc
     4861 ctgacggtcg gcgcccaggg caaggtcaag gccgacgtgt acgccaagga aatccacgtc
     4921 gagggcaccg tcgagggcga cctgttcggc gcggaacggg tggtcatccg caagtccgca
     4981 caggtgcgcg gcaacatcac ctcgccgcgc gtggccatcg aggaaggcgc ccgtttcaag
     5041 ggcgccatcg agatggactc cacggccgtc gaagccgcgc tcggcaagac ccgcggcgcc
     5101 gccggggccg cccgcgccaa gcccgtcgag ccggcggccg ccgcgaccac gaggagcggc
     5161 gccgccagct gagcggccga gggtgcctga gctcaacgcc ccgcttttcc gcggcctgct
     5221 gcagggaatg gaagatgatg cgcgccgggt cgtgctggac ctcggcccgg cgcgctcggc
     5281 caccgtggcg accttcggcc gctttcgctg ccggctcgag atcaccgacc tcggcgctgc
     5341 cgtcgacacc ctgaacgcca ccgaagagac tgcggaactc cgtgcggcgc tgcgggccct
     5401 gctgccgccg tcgcgcccgg aggcgaccga cctcgtgttg tgctgggacc tgctgaacta
     5461 cctcgagccg ccggtgctca aggccttgat ggacgaaatc cagtcacgtc tgcggcccgg
     5521 ctcgctggtg cacgccctga tcgcctactc gacgccacgg atgcccgccc ggccgcaaca
     5581 catcactccg acacccggct acgggctcga gatcgtgccc ctcgatggcg cggagcgggc
     5641 ggcgcggcgc tatacccccg acgagctcgg gcggctcatg ggcgggtacg tggtggagcg
     5701 cgccatgctg ctgaaaaacg gcatgcagga attcctcttc cgccactagc ggcccgtgca
     5761 cgccgcacgg ccggcctcac agccagcgcc gcacctgccg ctggtaggcc aggaattcct
     5821 cgccgaacac gatggccagc gcgcgctcct cgggccggat ctgcagccgg ttcatggcca
     5881 gcaggaacac cggcggcacc agcagcgcca agccattgcc gagcaggacg gcccaggcca
     5941 gcagcgccag cagcagcgcc aggtacatgg gattgcggct gcgccggtaa atgccgtggg
     6001 cgaccagccg cgtcgcgctg ccgggccgca acggattgac cgtggtctcg gcccgcgcga
     6061 aagcgcgcac ccccgccatg ccgaccaggc cggccaccag caacaccacg ctggcggcca
     6121 gcagctggcc ggggaaggcg acgctcgctg ccggaacgag ccacgccagc agccacatga
     6181 tccccgcggc gacgaggaac tgcgcgaccg gcggcacctt cagctcgagc gcgcgcaatc
     6241 gctcaaccgg gcggcgccgg gaactgcacg aagggaccct gcggttcctg gtcgggcggc
     6301 acccaggccg gcgccggctc ccctgccggc ccgaggtggc gcagcaggcg atacaaggca
     6361 accaggtcct cgtcgctcat gtcgcgcagc gcgaaccacg gcataggcgg ccggtagcgc
     6421 gccgtgcgcg ccagctcgat ccactgctgt tcggtgatcc gggcagccag caacctgagg
     6481 ttgcccgggt aagtcgtgcc ccacggcccg cgccatccca gcgcgtctcc cttgagccac
     6541 tcggactcgg gcaccatgcc gcccgactgg gggtagcccg tggtatggca atcgttgcag
     6601 ccggccaccc tggccaggta gcggccgcgc tccagcaccg ccccgtcctc ctccgcatgc
     6661 gccgccgacg ctatacaacc aagcgcgacc gccagcgccg caacaagcaa atgcgcatga
     6721 attttcatca cacactctcc ctggtcgttc tttttgtacc ggaaaccgtc acaagacgcc
     6781 ggctagagca tagcccaggc cggcgcgctt gcagagagtc cggggccgtc agccgccgcg
     6841 ccgccggcgc agcatgcggc caccaagcac cagcagcagg agcgcgtcga acaggccggg
     6901 cgatcccgca ccacccttgt aatccggcgt ccttaccacg cggtattcat ccgggttgct
     6961 gcgcacgcga tcctggaaga ccaccagctc ctggtcgagg ttgccgatca tgtcggcgac
     7021 cgcggcctcg aagccacggc cggcgtccag ccgcagctgc tcggagagat tgaccggcgt
     7081 ggccgtgcct gccacgaagc tcgtgccggg cgcacggaac agcatcttgc ggctcgggat
     7141 gtcgataatc acggcgtcca ccatcgtgtg ggtggaattc ttttcgccgc gcacgatgta
     7201 ggccccaatc agggtccagt aggcgagcga gctcaggcct tcgtcggtga actgcgcctg
     7261 gtcgtaggac accagcgcga ccacgtccac gccatgcatc gtgcgcagtt gctcgaggtt
     7321 ctggaatccg ccctgcgggc gcaggtaggc cgaggggatg atctcgatcg cgcggacgaa
     7381 ctcgcggtcg cggaaatgcc cggcgacctc ctccagcaac tggcgcttgc gcgcctcgct
     7441 gagcacggtg ctgccgcggc catgtgcttc gggcacaaaa gcaacaccga cgcgcatcgg
     7501 caggttgagc accgggatgc cctggtcggc caccggttct ttcgcatccg gatagagaaa
     7561 atcaacgatg ctggtggtct gccgcgactg ggtcgtcgca caaccggtag ccagcagcgc
     7621 cacgcccagc aggaacacga cgccgagacg aagttgagtt gatggttcaa gcattggccg
     7681 acctgcattg attgactgcc cggggactgg gccggtggca attctgcgcc gtgtcgcgtc
     7741 gatgcgccag acgatcggag accctgttgc gccgccttgg ccggtcaggc cctcagctgc
     7801 gcggctggct ccacttcccg ccgcggcgca tctcgcccca cttcgcctgg ccgcccccaa
     7861 gccagcgcag caggccgacg agccgccaca ccgcgttgag ctgccggtaa ccgaaattct
     7921 ccaggatggc gacggcgaac agcaccagcg tgctgcgcag ccccggatag acgtggaagg
     7981 acagttcctc cagcagcagc gcgctggtcg agagcaccac gcccagcccg acggccacca
     8041 gcaggaacac cagcgaggcc tccagcgaca tccagccgtt gagcgcggcg atgatgatga
     8101 ataggtaacc ggcgacttcc agcagcggcc cgaacagctc gaacagcagg ttgaacgggt
     8161 aggcaaagcg gccgaccacg ccggcgcgcc gctgggtcat cagcccgatg ttcttcgcca
     8221 ggctctcgcc gaggccgcgc tgccagcgga tccgctggcg cgccagcgtg cgcaggtctt
     8281 ccggcgcctc ggtccagcag atgggatccg gcacgaaagt gatccggtag ggcttgccgg
     8341 cggcccgcag cacgcgatgc aggcgcacca ccagctccat gtcctcgccg accgtgtcgg
     8401 tgcggtagcc accggcggcg acgacgactt ccttgcggaa caacccgaag gccccagaga
     8461 tgatcagcag cgcgttcatc ggcgaccagc cgaggcgacc gaacaggaag gcacgcaggt
     8521 attcgacgat ctgcagcagg gcaagcggcg agcgcggcag tcgcacccgc tcgacgaagc
     8581 cgtcgcgcac cgtggagccg ttgacgatcc gcaccgtgcc gccgcaggcg acggtgcgcg
     8641 gatcgtcgag gaagggctgc agcacgcggt acaggctgtc cgggtggaga atcgagtcgg
     8701 cgtcgacgca acagaacaag ggatagcggc tggcgttgat gccggcgttg agcgcgtcgg
     8761 ccttgccgcc gttggccttg tcgatgaccc gcacgttgtc gtgcagggtc gagcggtaga
     8821 cccggcgcac ttctgcggtc gccaggcgct gccggtaggc ctcggggaac ggctgcagcc
     8881 ggaaggcgtc gatcagcgcc tgcagcgtgc cgtcgcgcga gccgtcgttg atcaccacga
     8941 tctcgtattc cgggtagcgc agctgcagca gcgcgcgcac cgaggccacg atggtggcct
     9001 gttcgttgta cgccggcacg atgatggtga tcggcatctc gtagtcggag tacgcgcgcg
     9061 gcagcacgtc cacgcggcgg cccaggaagt actcgcgcag cgcgaacaag gcgttgaaat
     9121 tcagcaacag gtaggcgccg ttcatcagca cgaaatagcc gaggaacacc cactggccgg
     9181 tttccaccag ctcgttcagc acgccgcgcg ctccgccatc acgtggtcga ggatgtcgcg
     9241 ggcgaactcg tctcccatgg cgtcgcgggt ccgggccact tccgccagcg tcacgaaagg
     9301 catcgccagc agcgcctggg ccgcacggta gcgcacccac cactccgggt ccgacagcat
     9361 cgcgaccagc gtgtcactgt cctgcggcag ccccatgcgg ccgagcacca ccgccacgtt
     9421 catgcgcacg tgccagcgcg gatgcgcggc gagatcgcgg accaccggca ggtcgcgcgg
     9481 gttgccgagg atgcgcaggc agatcgactt gatgcggtcg tcggtcggcg cgcgcagcag
     9541 ccggctgacg atccgctccg acaccggcaa ggccacctcg ggcagcacgg caatgagctt
     9601 cggcacctgc tccggcgggc aggcggcgat ggccgcctcg agcggcgccg acaccgcctc
     9661 gggtcccgcc tccatgaaca ggtctgcaac ccgtggcgtg gaccagtcat cgcggtcccg
     9721 gatgaccggc aggacccgcg gcaacgccgc ttccgggtcg atgctgatca gggcgcgcgc
     9781 cgccaccagc gacaccagcg tgttctcgct gccgagcatc gcgagcaact cgttccaggc
     9841 acccgaatcg cgcaggtgac caagcgtggc ggccgcgagc agccggtcgg cgagccgccc
     9901 cgtgcgcagt agctgtcggg cataacgatc aatgccgagc cgggccgcca gcgcattgag
     9961 tcgcaacttc ggctcgccgc gcagcaggtc gtgcaggatg ttccattcga cgaggaacag
    10021 gcgacgatcc cgcgagggca acgcaggcag ggccgggccg gacgaatcat ccccgccacc
    10081 gatcgcgacc tcggcgaata ccgggcgcca gcgggcattg aaagccgcgc gccgccgttg
    10141 cgagcgcatc agccgggccc gcagccagag tatgccgagg aataccagca gggcgatgga
    10201 caccgcgcca agccccgtca gcagcgcgac ccgcagcagc aggtcagaag tcgtagcgaa
    10261 gcccggcacg gaaaccctcg cgcgtgtaga tgtcgccctg ctcgtgcacc agcagctccc
    10321 agctgacgct ccagtgcggc gccacgcggt gcagcccgag cagcgcgagt cccagcgtgc
    10381 tgctggtcag gaagcgcccg tcgccgatgc tttcggtttc gcgcccggcg gcggcgatca
    10441 gtccgatgcg actgtccgac gcatagtatc gatcgagtcg cgcatgaccg ctccaggtcg
    10501 tgtccgcgcg ctgcaggcgg ctggcctgca ccgtgagagc cgcgtgccac gggccggtgt
    10561 agcgctccgc accgacggtg gccgtggcga gatcatcgcc ggcatagccc gactgcgaca
    10621 cgccgccgta gacgccccag ccgtcgccga tgtcgcggtg cagccgaatg cggccggacc
    10681 agcgcggcaa cacttccgcg ccgggcgcga cgctgccctc cagcacgaat gaccagtcgt
    10741 cgttccaggg gcgcgcccag cccgcgcgac cctcgacgtc ggaagcatcg aagcgctcct
    10801 cccgctgaac gctgccgaac acccggtagc cgagcgcgtg gctgcgctgg tactcaatgt
    10861 actggctgcg ccagtcgggc aacccgctgc caaggtcctg ccagccgagc ccggcctcca
    10921 gcgactcggc cggccgcgcg gcgagccgtt cgcgcacctg cgcctccagc cggatgaccg
    10981 cctggtagtc cggtgcgacc tgacgcgcct cctccagcag cggcagcgct gccgccggcc
    11041 gtcccgacca ggccagcacc tgcgccttgc ccagcaggta gtccggattg ccgggcgccg
    11101 ccgcgagcag ccggtcgtac tcgcgcagcg cctcgtccca gcgctctgcc caggagagcg
    11161 cccgggcgta ttcgaaacgc gcctccagcc ggccgggatc ctcggccagc tgcgcctcca
    11221 gctccgcgag ccgtgcatcg tccggcatcg ccgcgccgac cggcagcagc gccagtccga
    11281 gcaagaggac caggacccgc gccggcatca agcccgcgct cccgtagcgg tctgcatgtg
    11341 cctgcggatg cgcgccagca gcacgccggc attgaacggc ttggtgacgt agtcgttggc
    11401 gcccagctcg agcgcgcgga tcacttcctg gtccgcgctg tgcgccgaca gcacgatcag
    11461 cggcaccgcg cgccagcgtt cgtccgcgcg gatgcgcgtg atgagctcga agccgtccac
    11521 gaagggcatc acgatgtctg tgacgacgac gtcggcgggg ggctcgcggt cgatgtgctc
    11581 ggcggccgag gccccctccg ggaacgaggc cacgacgaag ccttcccgct ggagcatgta
    11641 ctccagcagg aaggcggtgt gctcgtcggg cccgatcacc agggcgcgcg gcgaatcctg
    11701 cagcagtggc ggcgcggcgc cttcagttcg cgtcatgccg cgaagagtgc cagaactctc
    11761 cggcggggcc tatgcgccag tcctcaattc cgcgtgtgcg caagcatgac gccggacgtc
    11821 ggctaggcct cgtggcaggc cacccggtgc tcgccgaccc gccgcagcgc cggggtctcg
    11881 atggcgcatc gctccacggc gatggggcac cgcgtgcgga aggcgcagcc cgagggcggg
    11941 tcgaccggcg agggcaggtc gccctgcagc ggggcgtacg gcttggcacg ctccttctgc
    12001 gggtcgggga tgggcaccgc gcggatcaac gcctgggtgt aaggatgcat gggttgctcg
    12061 tagagcgcgt tgcggtccgc gatctcggcg atccggccca ggtacatcac catgatgcga
    12121 tggctgatgt ggcgcacgac ggcgagatcg tgcgcgatga acaccagcga caggttcatc
    12181 tcggcctgca ggtccatcag caggttgacg atctgcgcct ggatggacac gtcgagcgca
    12241 ctgaccggct cgtcgcagat gaccagtttc ggctgcagca ccagcgcgcg ggcgatgccg
    12301 atgcgctggc actggccgcc ggagaactcg tggggatagc gattgacgtg ctcgggcccc
    12361 agtccgaccc ggcccagcat ggcctcgacc ttctggcgca cttcgcggcg cggcatcgcc
    12421 gggaaatgcg tccacagcgg ctcggcgatg atgccgccaa cggtcatgcg cgggttcagc
    12481 gaggccagcg ggtcctggaa gatgacctgc atgtcgcgcc gatgaatgcg catcgccttc
    12541 ttgtccttgg cggtgatgtc ctcgccgagc aggcagacgc gcccggtggt ggcgggcacc
    12601 agccgcagca cggcacgcgc cagggtggac ttgccgcagc ccgactcgcc caccacgccg
    12661 agcgtctcac cggggcgcag gtcgagggcg acgccatcga ccgccttgag cagccgcggc
    12721 cgggcgaagg tggtctcggg tatcagccgg aagtgcaccc gcaggtcgtc gaccaccagc
    12781 agcggcgggg ccgcgtagtc gggctcgcga cggacgaccg gcgccgctgc cgctgccgct
    12841 gccgccccct gcaacgccgt gccgtcggcg ctggtggcgg aagcatccag cgaggccagc
    12901 cgcgtggcct ccgcccggtc gagacgcggc acggcagcca agagcgcgcg cgtgtaggcg
    12961 tgtcgtggcc ggtagaaaat gtcgtccacc ggcccggctt ccttttcctc gccgccgtgc
    13021 atcaccagca cgcgatcgca cagcccggcc accacgccga gatcgtgggt gatgagaatg
    13081 atgctggtcc cgaaatcacg gcgcacctcg cgcatcagct cgagaatctg cgcctgcacg
    13141 gtgacgtcga gggcggtggt cggctcgtcg gcgatcagca ggtccggccg gcacaacatg
    13201 gcggacgcga tcatcacccg ctggcgcatg ccgccggaga attcgtgcgg gtacatgtcg
    13261 acgcgcttgg cggcctcggg aatgtgcacc ttctcgagca gctggatgca gcgcgcgcgg
    13321 gtctgcttgc ggccgagctt ctcgtggtgc gccagcacct gcgccatctg ctcgaacacc
    13381 gtcatgtacg ggttgagcga ggtcatcggg tcctggaaga tcatgccgat gcgccgcccg
    13441 cgaatgcggc gcagcgccgc gacgggcatg ccgatgagct ctgcgccgtc gaagcgcgcc
    13501 gagccgctgg cgcgaccgtt cgaggccagc aggccgatca ggctcatcat ggactggctc
    13561 ttgccggagc ccgactcgcc gacgatcccc agggtctcgc cgcgttgcag cgagaaaccg
    13621 agatcgcgca cggcgtgcac ctcgccgtcc ggcgtgtgga agcgcacatt gagcttgtcg
    13681 acttcgagca gcgccatcag cggtccttcg gatccagcgc gtcgcgcagg ccgtcgccga
    13741 cgaagttgaa gcagaacagc gtcaacgcca ggaacaccgc cgggaagacc agcatccacg
    13801 gcgcggtgcc catctcttgc gcgccctcgt tgaccagcgc gccccacgag gtcatcggct
    13861 cctgcacgcc gaggccgagg aaactgagga aactctccac caggatcacc tgcgggatgg
    13921 tcagcgtgac gtacaccacc acgaccccga gcaggttcgg gacaatgtgg cggcggatga
    13981 tgttgaaggt agacacgccc tgcgcctcgg ccgcctcgac gaactccttg tgcttcagcg
    14041 acagggtctg gccgcgcacg atgcgcgcca tgtccagcca gttgatcgcg ccgatggcga
    14101 cgaagatcag gatgatgttg cgcccgaaga acaccatcag caggatcacg aagaacatga
    14161 agggcatggc gtagagaatg tcgacgatgc gcatcatgac gtggtcgacc cgcccgccga
    14221 gatagccggc ggtggcgccc cagctgatgc cgatcagcag gctgaccagc gtcgccacca
    14281 cgccgatcag caaggagatg cggccgccat agagggtgcg cacgaacagg tcgcggccca
    14341 gcgcgtcggt gccgaagtaa tgccggctct cccagtcggg cccgatcgag gtgtttgccc
    14401 agtcggtgtg gtcgtaggcc cagggcgaca gccagggacc gacgatcacc agcaccgcca
    14461 tggtgaggat caacgccccc gcgaccaccg cggcgcggtt gcggcgcagc acctgccagg
    14521 cgtcgatcca caggctgcgg cccttcaccg gttcgatgcc ggacccgccc gacgggggta
    14581 acaactgcgg cacggcgctc atgcgtactt caccttggga tcgagccagg cgtagaccag
    14641 gtcgaccagc aggttgaaca ggatgatcag cgcgccgtag aacaccacca cgcccatcac
    14701 cagggtgtag tcgcggttca gcgcgccctg cacgaaatac cgccccagcc cgggcacgcc
    14761 gaagatctgc tcgatgacca ccgagccggt gatcaccgcc gccgtcgccg ggccgagata
    14821 ggagaccacc ggcagcagcg ccggcttgag ggcgtggcgc aggatcacgg tgcgctccgg
    14881 cagcccctgg gcccgcgcgg tgcgcacgaa gttgctgcgc aggacctcga tcatgctgcc
    14941 gcgggtcagc cgcgagatgt aggccacctg cggcagcgca agcgagatca ccggcagcag
    15001 catgtggcgc agcccgccgt cgccgatgcc gcccgccggc agccagccga gcatcaccgc
    15061 gaacagcagg atcagcagcg gcgccatgac gaagttcggg atcgagatgc cggtcatcga
    15121 gacggccatc accacgtggt cggtgcggga gttctggcgc agcgccgcga tggttccggc
    15181 ggtcacgccg atcagcagcg ccagcagcat ggcggagccg ccgagccgca gcgaaaccgg
    15241 gaagccggcc gcaatcagct cggtgacgct gtagtccttg tactggaacg aaggcccgaa
    15301 gtcgccgcgc accaggttcc agacgtagcg tccgaactgc tggatgagcg gctcgtcgag
    15361 atggtaggcg gcgcgcaggt tggcctcgat ctcggcgggc agcgccttct cggcgtcgaa
    15421 aggaccgccg ggcgcggcgc ggatcatgaa gaacgcgatg gtgatgagga tgagcagcgt
    15481 cgggatggcg ccgaggaagc gggtcaatgc gtagcgcagc acgtcgccct ctccttccgg
    15541 ccggtcatgg cgtcatccct cgggtccatc agtgcttcag gatcttcagg tctttgctgt
    15601 agacgatgtc ctggatgtgc ggccggtaat tgccgaccca gggcttcacc agccgcttgg
    15661 tgacgtagaa atagatcggg atcagcggca cgtcgtggat cagcacgcgt tccgcctcgt
    15721 agaggtactc ggcacgccgc gccatgtcgt tctcctgcgc ggccagcgcc agcagctcgt
    15781 cgtaacgcgg gttattccag ccggagtcgt tgagcccgct ctgcgagtgc atcatctcgg
    15841 caaaggtatt ggcgtcgttg taatcgccga tccagccggc gcggaacacc tgggtggtct
    15901 gcttggtgcg ccgggtctcg aggaacacct tccactcctc gttgaccagc tcggtctgga
    15961 tgcccagcac ctcgttccac atggccgaca cggcaaccga gatggtgcga tggttctcgt
    16021 gcgtgttgta gaggatctgc agccgcaacg gccggtcgcg cgaatagccg gcggcctcgt
    16081 acagtcggcg ggcttctgca ttgcgctctt cctgggtcca gctcgcccac tccggcacgt
    16141 actggtggta gttcgcagcg tgcggcaccc atccgtacgc cggaatctcg cccgccgtgg
    16201 tgacctggct ggtgatgatg tcgcggtcga tggcgagata cagcgcgcgg cgcagctccg
    16261 ggttgtcctt gaacggtggc cgggtcacgt tcagcccgaa atagtagatc ccgaggtact
    16321 cgtggatctg cagctcctcg ggcaggttcg cccggatcca cgcgagctgc cggaagggca
    16381 aatcgtaagt gtaatccagc gcgtcggcgc ggtagcgctg cagctcggtt gactggtttt
    16441 cgatcgggta gtaccagacc tcctccagcg tgacgttggc ggcgtcccag tagtgctcgt
    16501 tgcgctccag cacgatgtgc gactgcatca cccagtcgcg cagccggaag gcgccgttgg
    16561 acaccagccg gccggggcgc gcccagcggt cgccgtagcg ctcaacggtc gccctgtgca
    16621 ccgggtagct cgccgagtgg gtcagcacgc cgagaaagta cggcgtcggc ccggcgaggc
    16681 ggatctccag ggtcaaatcg tcgatggcgc gcacacccag cgcctccggc ggcaattcgc
    16741 cggcgatgat ctgctcggca ttgagtatgg gccgcagcat gatgctgtag ttagacagcg
    16801 tctgcgggtc gaccgagcgc cgcaggccgt actcgaaatc gtgcgcggtg accgggtcgc
    16861 cgttcgacca gcggccgtcc tcacgcatgt ggaagaggta ggtcagcccg tcctcgctga
    16921 tctcccaggc ccgcgccgca cccggcacga taccgtccgg cgcatcgttg gtgagaccct
    16981 cgtagaggtc gcgctgcagg ttggactccg gcaccccttc gctcttgtgc ggatcgagcg
    17041 actggggctc ggcgccgttg ccgcggtgca ggacctggcg ctccgccagc tcggcgccgg
    17101 actcaccgcc gatgcggacc ggcgccgttc gctccgcgcg cgacgccgga tcgccgcagc
    17161 cggcggccag cgacatcatc agcaggaggg gagcgatcca tcggcaacgg gtcatcaggc
    17221 tcggtgtcat ggaaaatcgg ctgcggcaag gtatcacagt ggggggggac cagctaaatc
    17281 ggaccgcgcg gcgtccccgg gggttcccgc cgtcacccgc gtcgctgccg gttcg
//
LOCUS       DAOMPG010000020        17190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1472_length_17190_cov_4.390662, whole genome shotgun sequence.
ACCESSION   DAOMPG010000020 DAOMPG010000000
VERSION     DAOMPG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_17190_cov_4.390662"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PLS34_01965"
     CDS             complement(<1..1746)
                     /locus_tag="PLS34_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HRP86271.1"
                     /translation="MTGDALLLALLLGPLAGALAVPWLGRRSERLRDGWVLLVTGLVL
                     LGTVLLASRVLAAGRLSAGLSLMAGRLEFTADPVGALFALFSAFVWFCATLYSIPYMK
                     TQRSRDRYHAVSLVLLAAMLGVVVAADLVTLFLCFEALGLVAFLLVVHTGTPEAHRAA
                     IQYFWMTVLGGIALLSGIMLVYAMGGGPLLPGSSLGAGHRGAAATLLVLGFGVKAGMV
                     PVHFWLPNAHPVAPTPASALLSGVMIKAGAYGIYRSLAALFTPPQGTAYADAAWAFTA
                     QFGLVVTWIGIVTMAVGVVLALGQSNAKRMLAYHSISQMGFILAGLGAGAFLLGDGSM
                     GTAGGLMHTVNHALFKASLFLGVGAVAFRTGSLDMYALGGLWRRMPLTFLLMLVAAAG
                     ITGLPLFNGFVSKSMIHHALEAAGAESGLASLRVAERIFVLTAAGTAASFIKLIGLVF
                     LGPCKLPRPEEVREAPGAMLLAMALLAVPVIALGWRPGLLLEGMLVPAMLAGGVPTDG
                     IAHYLGHYFLSAGDVWMSLLVFAGGGLVFAIGMRFGLFHLHLPARLSPAYWFGRAGLG
                     VVAICEQGSMARHDGH"
     gene            complement(1762..3240)
                     /locus_tag="PLS34_01970"
     CDS             complement(1762..3240)
                     /locus_tag="PLS34_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP86272.1"
                     /translation="MSLLPALIVALPLLGGVLALPLRRLGERRRALALCMLCGAAMLG
                     VFALVPGALRGEVRELALVHFAPQVELYLRVDALGALFAATVATLFLLALVYSFRYLR
                     RDGHETRYFVFFMLSLGWMLGVAFAGNLVTLLVFYELFSILVYPLVVHEETPEAIRAG
                     TKYIVYILVGGSLILLGVIFCFYLAGNQPLTPGGILDPATDRTLLLVTFWCLVAGFGV
                     KAALMPLHGWVPDVHPAAPASFSALLSGVMVATGCFGILRVIYEVFGVELVAALGVAP
                     VLTAVAAISVLLAAILAAGEDNLKRRLAWSTISQMAYVVLAVSLLAPRVSVGAMVHIS
                     NHAFMKGALFFCAGLLASQAGIRRVSEMAGVARRMPLTMAAFTIAALGIVGVPPLAGF
                     ISKWVLGVGILEAGHPLVLLVLLGGALLAALYLLQPVWMAYFHAAPDSVAERPRPEAP
                     VSMLVPLVLAALLSILLGLLAAMPGLPLSLAERAAAVFFGAE"
     gene            complement(3255..4703)
                     /locus_tag="PLS34_01975"
     CDS             complement(3255..4703)
                     /locus_tag="PLS34_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP86273.1"
                     /translation="MSLACAILIFMLGRRDLWRAVFALGASALKLAIVLSMLPGSLAG
                     TVYVYDLVDFPPGIGIAFRADGLGMFFALVSSTLWLVTTVYAIGYMQGSHERVRFFGF
                     FALCVSTTVGLAFAENLLTLFLFYELLTICTYPLVIHDETPEAMKAGRKYLAYTLSGG
                     AAVLLGVVLVQHLAGTLTLSMPGVLSLDAGRPMLLVTFVALAGGFGVKAAIMPLHGWL
                     PTAMVAPVPVSALLHAVAVVKAGVFGLLRVIYNVFGIKLLGVLGYTDVLALFAGFTII
                     AASLMALAQSNLKLRLAYSTVSQLSYIVLAASLLTPLAALAAIAHIANQAFAKITMFF
                     VAGAVQRTTGRTQIHELAGIGRVMPWTMGAFTIAALSFIGVPLFAGFVTKWFLSLGAL
                     QAGAWWWVLLMVASSLLNAAYWLPILYLAWFREPRGLPPGIHEARAMLLGPILVSAAY
                     VLLLGMTSRIPGMPFSLAQATVRFVTGIQGGT"
     gene            complement(4747..6222)
                     /locus_tag="PLS34_01980"
     CDS             complement(4747..6222)
                     /locus_tag="PLS34_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP86274.1"
                     /translation="MAHWLPLAAVLPLLAGALTPALAAGRPGLARGWALATAVFCTVV
                     TAVLTHRVAQEGPFSYAMGGWPPPWGIELRFDEFSAAALFISFIMVLVLVYSGPYVRR
                     AVPAQRIPWYYSLLLVNLGAMIGFVVTADLFNLFVFMELVAVSSYALVAVGGGRTAAL
                     AAFKYLMAGSVSSALILLCIGVVFALTGSLNMADIASRLAEVESRAPVVLALVGMTVG
                     FLVKAGLFPLHVWLPDAHAIAPSPINAVSSALVVKLGIIGVLRVWPLFAAAATVSLAP
                     LYEALAWLGAVSILAGAFFALFQQDIKLMLAYSTISNIGYIVLGLGLAGELAVTGATV
                     HVFNHALIKATLFLAAGAMLHQSGYRTLSDLRGIGHAMPWTSLALLIGVLAIAGLPPT
                     AGFLGKWYIALGAVEAGRPGFAVVVLLGALLVFIYLGRMLNAFYFRAPIHEQMLAVRE
                     APAAILWPVLLLAMLCVLAGLLGRWPVALIAPAVTRLSGGG"
     gene            complement(6222..6572)
                     /locus_tag="PLS34_01985"
     CDS             complement(6222..6572)
                     /locus_tag="PLS34_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRP86275.1"
                     /translation="MTGLWQNIDYAAFVVLFCIGLYVIIAKQNLIKKLLGLNIMETAV
                     FAFVVTSGMVDGGTAPILGADATAPFASPLPHALILTGIVVALSITALALCLIIEIYR
                     HCGTIEADELKGAE"
     gene            complement(6574..7293)
                     /locus_tag="PLS34_01990"
     CDS             complement(6574..7293)
                     /locus_tag="PLS34_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRP86276.1"
                     /translation="MRKTLVALTIAAAAVPMLIGVAALPRPGDGGAPAHTHVAARYLA
                     DGAREAGADNLVTGILLNYRGLDTFGEVTVIFTALAAAMAVLTPAAPGGTGGVPPGGP
                     PGAPLSPVVAYVVRLTAPLIALFGAFVILKGHVSPGGGFQGGVILGALLMLLSVVMGR
                     GPRAPLVPTVAARWLQAAAPLTFAVVGLLGLALTGALLGFPDDPGMALLRELMMIGLE
                     LGIGIGGAAVIFGLFVGMRGD"
     gene            complement(7290..7532)
                     /locus_tag="PLS34_01995"
     CDS             complement(7290..7532)
                     /locus_tag="PLS34_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRP86277.1"
                     /translation="MSQVFDLVMLGLLIAMALAVARSKDLVAAAIIFSGYSLVMCLLW
                     QHRGAPDVAMTEAAVGAGVTTILVLVAIGRTCRKER"
     gene            complement(7529..7852)
                     /gene="mnhG"
                     /locus_tag="PLS34_02000"
     CDS             complement(7529..7852)
                     /gene="mnhG"
                     /locus_tag="PLS34_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRP86278.1"
                     /translation="MMGAALHSLTVLLALVGAGFFVAGTLGLLRLPDFYTRAHAVAKC
                     DTVGAGAILLALAVHVAPHPEALKILLLLALVLISSPTAGHALAHAAHGTGLESWEPS
                     AGDAP"
     gene            complement(7849..8127)
                     /locus_tag="PLS34_02005"
     CDS             complement(7849..8127)
                     /locus_tag="PLS34_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRP86279.1"
                     /translation="MTAFFWGLAIFLLLNATLCVWRAFAGPTVVDRILATNVVGTKAL
                     IVLVLLGLAFERSLLLDVAVVYGLLNFGITIAATRFLETGRLKGDWQS"
     gene            complement(8124..8633)
                     /locus_tag="PLS34_02010"
     CDS             complement(8124..8633)
                     /locus_tag="PLS34_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRP86280.1"
                     /translation="MANGPRSTPRFIAAGMVVFVFWLVISASLAWTDLAVGLVLSLLL
                     GGWSTRYLWAGDAPRLSMREAALLLRRSPGFAWQIFLSALHVARVVLDPRLPVRPRLV
                     TCRTRLRRDISRIAFAQAVTLTPGTLTVDLRGGDFLLHCLDEASAERILDGSLERQVA
                     RIFEPESPS"
     gene            complement(8833..10377)
                     /locus_tag="PLS34_02015"
     CDS             complement(8833..10377)
                     /locus_tag="PLS34_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRP86281.1"
                     /translation="MTRFLHVVEWLGNALPHPVTLFALFAFGVVLFSGLMGALGVAVP
                     DPRPVGADGRAADGMIRVVSLMNADGLRRIVQNLVTNFTSFVPLGTVLVAMLGVGVAE
                     RSGLLTAMVRAMVLRASPRMVTVTIVFAGVLSNTASEMGYVVLVPLAAMVYYSLGRHP
                     LAGLAAAFAGVSGGYSANLLVGTVDPLLAGITQEAARLIAPGYLVDATANWFFMVGST
                     FLVVIVVSWISLRIVEPALGPYDRGQARLGDDDEHRMDPLSDAERRGLRHAGIAALLM
                     VGLIALLVVPEGAVLRNPETGAIKDSPFLRGFVALIFVFFLAPGIAYGAAVGTMRSDR
                     DVINAMASAMSTLGLYMVLVFFAAQFVAFFGWTNLGAVTAVTGAELLKSIGLTGPLVF
                     VFFVLMCAFVNLMLGSASAQWAVTAPIFVPMLMLLGYSPEIIQAAYRIGDSTTNIITP
                     MMSYFGLIMAFAARYVPKAGIGTLISMMLPYSVALIICWTAFFFLWVFVLGLPVGPAT
                     ATYYTP"
     assembly_gap    10450..10459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10558..11001
                     /locus_tag="PLS34_02020"
     CDS             10558..11001
                     /locus_tag="PLS34_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86282.1"
                     /translation="MPALIALLLALVIGAFFVSRRAGMLMLAGASVAVAAMFAWTWHT
                     GPGVELRRDAIPVAEIEILDTRRRGNTTDYLVRNGSQGWTLTALYTERIARDAAGEVV
                     DRRTFKHIVEVPPGESSWQTLRFFGLEIGRQYELEVVGTEGARPR"
     gene            11012..11761
                     /locus_tag="PLS34_02025"
     CDS             11012..11761
                     /locus_tag="PLS34_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRP86283.1"
                     /translation="MARIMVLKHVAAEPLGLLDPMIRARGHRIRYVNFQRHPDAEPSL
                     DRYQALVVLGGPMNVGDTDRHPHLLTECRLIEQALAQRIPVLGICLGAQLLAHVLGAR
                     VAPARRPEIGWHLVRPRLPAAADDAVLHPLDAERPVFQWHSYGFDIPDGAAHLAESDD
                     CPGQAIGYDGCAWGFQFHLELDESLIRRWLGSPGYLRELAASGLDRTPDEILDDTRHH
                     LAATRVLAARVFGNWLDLLGQNGGRRLLPSR"
     gene            11791..13620
                     /locus_tag="PLS34_02030"
     CDS             11791..13620
                     /locus_tag="PLS34_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009287169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP86284.1"
                     /translation="MPITGVPDSPREFLGVFRYSRRALQLVWTTSRRLTVILAVVTVV
                     AGVLPAAIAFVGALIIDAVVAAARLYQESGAAQLREVMGLVAAEGLLVAALAGAQRAL
                     SASQSLLRAQLGQRVNVMILEKALQLELSQFEDSEFYDKLTRARREASSRPLSLVIRT
                     FGLAQNAVSLVSYGVLLAQFSAWAVLVLVLAGLPAFLAEAKFSGDAFRLFRWRSPETR
                     MQMYLETVLAREDHAKEVQLFGLGPRLLDRYRQIFQTLYREDRNLTLRRDGWGFVLGL
                     VGTLTLYGAYAWVALTAVLGRITLGQMTMYVMLFRQGQSAVSASLSAIGGMYEDNLYL
                     STLYEFLETPVRARGDTLAAGPQPGDGIRFEGVGFTYPGTEAPALQDIDLHIRPGQSL
                     ALVGENGSGKTTLIKLLARLYTPTAGRILLDGLDLERWDEHALRRRIGVIFQDFARYQ
                     LKVGENIGAGDEPRFEDRAGWLAAAEQGMALPFIESLPERFETQLGKWFKQGRELSGG
                     QWQKIALARAFMRSEADILVLDEPTAAMDAAAEATIFEHFRNLTRDRIAILISHRFST
                     VRMADQIVVIEGGRIIERGSHDELMALDGLYARLFSLQARGYR"
     gene            complement(13637..14962)
                     /locus_tag="PLS34_02035"
     CDS             complement(13637..14962)
                     /locus_tag="PLS34_02035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP86285.1"
                     /translation="MNERQDSTTGGPPGAAARPALRRPEFIYSLALFVAALAALSVAI
                     ADQGLLPPDAGPGLVAFFLVYALFTIFMGYTHPRVGYVSFDRIAQVAAILVLGPVAAA
                     WINGTASLLYPWQRLRRGRPFIEVLTAALHNAGLMSLMILVCGLLYQKLGGPVPLLAL
                     GWRDVATLLLLLLSMQALNDLGMRVLMTLEERRVPTDFSPFAFVVESGAGLGGILVAL
                     IINRMELSAVVLLLLVMTLGMFSLMQLAHIRTRLEALVAERTRKLQQKSLELERMASH
                     DPLTGLHNRRHADQYLEDRINEFERYGRDFAIALVDLDDFKRINDDHSHDTGDEVLRL
                     VAQLLRERCRDTDLVSRYGGEEFLLCFPQAGMVAAREACEKIRETVASTEWELLVPGV
                     RVTLSAGVATMREGVSRRELLGLADRALYEAKSGGRNQVRVAAERMRAL"
     gene            complement(14972..15691)
                     /locus_tag="PLS34_02040"
     CDS             complement(14972..15691)
                     /locus_tag="PLS34_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRP86286.1"
                     /translation="MTELGEIWVYLAAEPLLWLTVTLAIFVASTWLNHRLKGHPLAHP
                     VLIAMLVLIGLLLLTGTSYDTYFEGAQFIHFLLGPATVALAIPLYDNLRQIRRLLWPL
                     LFACLLGVTVAVSSAVGLAWLAGGRDQTLLSIAPKSVTTPIAMGIAEQIGGLPSLAAG
                     IVLLTGAVGAMAAGPLFRLAGIRDERAQGFALGIAAHGFGTAHALTIGLRAGAFAGLG
                     LGMAGLLTAFLLPLAVRLLAG"
     gene            complement(15684..16037)
                     /locus_tag="PLS34_02045"
     CDS             complement(15684..16037)
                     /locus_tag="PLS34_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRP86287.1"
                     /translation="MLQGLTLIFICQLAGEALVRLLGVPFPGPVAGMVLLLLLLVAAG
                     GPAAGVRRAGTGLLTYLPLFFVPAGVGLITHGARLRTDWFPILLAIVGSTLLTMLLVG
                     WLIARHAEPVEDASD"
     gene            complement(16229..16510)
                     /locus_tag="PLS34_02050"
     CDS             complement(16229..16510)
                     /locus_tag="PLS34_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86288.1"
                     /translation="MTAHVVLFLILGLLIGAVAAVPFAIPKDARGSTTKRGWVVYISC
                     MGICLGIAAAMGQISSHLRLDDQYNWIAAGFVISGLLVGIVILKWLRPL"
     gene            complement(16507..>17190)
                     /locus_tag="PLS34_02055"
     CDS             complement(16507..>17190)
                     /locus_tag="PLS34_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017385206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRP86289.1"
                     /translation="YNLGFPGQYYDAEGASWQNWFRTYDGSIGRYTQSDPIGLAGGLN
                     TYGYVGGNPVRFIDPTGEYAVLVPVGFGVVVGGTSALIAALNDPCSTPGSIALATAAG
                     MVVGGASGNLSVGGTVVSNIVRNGLAGATGNAIGQLLGSREFSGAQVLVQSMVGAVGG
                     NWGRAAGFTHGPRMLRSGATADQAISTSLAVGSVHAVGANGLQQMTLPSNFGGIGSTE
                     TSVACGCGK"
BASE COUNT         3166 a         6056 c         5790 g         2168 t
ORIGIN      
        1 gtgcccgtcg tgcctcgcca tggaaccctg ctcgcagatc gccaccacgc cgagcccggc
       61 gcggccgaac cagtacgcgg gactgaggcg cgccggcagg tgcaggtgga acaggccgaa
      121 cctcatgccg atcgcgaaga ccaggccgcc gccggcgaat accagcagag acatccacac
      181 gtcgccggcg ctgagaaagt agtgcccgag gtagtgcgcg atgccgtcgg tcggcacgcc
      241 gccggcaagc atcgcaggca ccaacatgcc ttcgagcagc agtccgggcc gccagccgag
      301 cgcgatcacc ggcaccgcga gcagcgccat ggcgagcagc atggcgcccg gcgcctcgcg
      361 tacctcctcg ggccgcggca gcttgcaggg cccaagaaac accaggccga tgagcttgat
      421 gaaggaggcg gcggtgccgg cggcggtgag cacgaagatg cgctccgcga cacgcagcga
      481 agccaggccg ctctccgcgc ccgctgcttc cagcgcatgg tggatcatgc tcttgctgac
      541 gaagccgttg aacagcggca gccccgtgat gccggccgcg gcgaccagca tcagcaggaa
      601 ggtcagcggc atgcgccgcc acagcccgcc gagggcgtac atgtccaggc tgccggtgcg
      661 gaaggccacg gcgccgacgc cgaggaacag gctggccttg aacagcgcgt ggttgacggt
      721 gtgcatgagc ccgccggcgg tgcccatcga accgtcaccg agcaggaagg cgccggcgcc
      781 gaggccggcg aggatgaagc ccatctggct gatgctgtga taggcgagca tgcgcttggc
      841 attggactgg ccgagcgcca gcaccacgcc gactgccatg gtgacgatgc cgatccaggt
      901 caccacgagg ccgaactggg cggtgaaggc ccaggcggcg tcggcgtacg ccgtaccctg
      961 cggcggcgta aagagcgctg cgaggctgcg gtagatgccg taggcgcccg ccttgatcat
     1021 cacgcccgac agcagggcgc tggccggcgt cggcgccacc gggtgggcgt tgggcagcca
     1081 gaagtgcacc ggcaccatgc cggccttgac gccgaagccg agcaccagca gcgtggccgc
     1141 ggcgccgcgg tgccccgcac cgagcgagct gcccggcaac agtggaccgc cgcccatggc
     1201 gtacaccagc ataatgccgg acagcagcgc gatgccgccc agcacggtca tccagaagta
     1261 ctggatcgcg gcgcggtggg cctccggcgt gccggtgtgt accaccagca ggaaggcgac
     1321 caggccgagg gcctcgaagc aaaggaacag cgtcacgagg tctgcagcca ccaccacgcc
     1381 gagcatggcc gccagcagca ccaggctgac agcgtggtaa cggtcgcgag atcgctgcgt
     1441 cttcatgtac gggatcgagt acagggtggc gcagaaccac acgaaggcgc tgaacagcgc
     1501 aaacagggcg ccgaccggat cggcggtgaa ctccagccgc cccgccatca gcgacagccc
     1561 ggcgctcagc cggccggcgg cgagcacccg ggatgccagc agcaccgtac ccagcaggac
     1621 caggccggtg accagcagca cccagccgtc gcgcagccgc tccgagcggc ggccgagcca
     1681 gggcaccgcc agcgcgccgg cgagcggccc gagcagcagc gccagcagca gggcgtcacc
     1741 ggtcatgctc gctgcgcctc ctcactcggc accgaagaac acggccgccg cccgttcggc
     1801 cagcgacagc ggcagtcccg gcatcgcggc gagcaggccg agcaggatgc tcaacagggc
     1861 ggccagcacc agcggcacca gcatgctgac cggtgcctcc ggccggggcc gctctgccac
     1921 ggagtccggc gccgcgtgga agtacgccat ccagacgggc tgcaacaggt acaacgcggc
     1981 cagcagcgcg ccgccgagca gcaccagcag caccagcggg tggccggcct cgaggatgcc
     2041 caccccgagc acccacttgc tgatgaatcc cgccagcggc ggcacgccga cgatgccgag
     2101 tgcggcaatg gtgaacgccg ccatggtcag cggcatgcgg cgcgccacgc ccgccatctc
     2161 ggagacccgc cgtatgcccg cctggctggc cagcaggccg gcacagaaga acagcgcacc
     2221 cttcatgaag gcgtggttgc tgatgtgcac catagccccg acggaaaccc gtggtgcgag
     2281 cagcgatacc gccagcacca cgtaggccat ctggctgatc gtggaccacg ccagccgccg
     2341 cttcaggttg tcctcgccgg cggccagaat ggcggccagc aggaccgaga tcgccgccac
     2401 ggccgtcagc accggcgcca cgcccagcgc cgccaccagc tctacgccga acacctcgta
     2461 gatcacgcgc aggatgccga agcagcccgt cgccaccatc acgccggaca gcagcgccga
     2521 gaaggaggcc ggcgcggccg ggtgcacgtc aggcacccag ccgtgcagcg gcatcagcgc
     2581 cgccttcacg ccgaaacccg cgaccaggca ccagaaggtc accagcagca gcgtccggtc
     2641 ggtcgccggg tcgaggatgc cgcccggcgt gaggggctgg ttgccggcca ggtagaagca
     2701 gaagatcacg cccagcagga tcaggctgcc gccgaccagg atgtagacga tgtacttggt
     2761 gccggcgcgg atcgcttccg gcgtctcctc gtgcaccacc agcgggtaaa ccaggatgga
     2821 gaacagctcg tagaacacca ggagcgtgac caggttgccg gcaaaggcca cgccgagcat
     2881 ccagcccagg ctgagcatga agaacacaaa atagcgcgtc tcgtggccgt cccgtcgcag
     2941 gtaccggaac gagtacacca gcgcgagcag gaacagcgtg gccaccgtcg cagcgaacag
     3001 cgcacccagg gcgtcaacgc gcaggtacag ctcgacctgc ggcgcaaagt gcaccagtgc
     3061 gagctcccgc acttcgccgc gcagcgcgcc gggcaccagg gcgaataccc cgagcatcgc
     3121 cgcgccgcac agcatgcaga gcgccagcgc ccggcgacgc tcgcccaggc ggcgcagcgg
     3181 cagcgccagc accccgccga gcagcggcag cgccacgatg agcgccggca gcaggctcat
     3241 ggccgttgcc gacctcacgt gcccccctgg atgccggtca cgaagcgcac ggtcgcctgc
     3301 gccagcgaga acggcatgcc gggaatgcgc gaggtcatgc cgagcaacag cacgtaggcc
     3361 gcgctgacca ggatcggccc cagcagcatg gcccgcgctt cgtggatgcc cggcggcagc
     3421 ccccgcggct cacggaacca ggcgagatac aggatcggca gccagtacgc cgcgttcagc
     3481 agcgagctcg ccaccatgag cagcacccac caccaggcac ccgcctgcag cgcacccagc
     3541 gacaggaacc acttggtgac gaaacccgcg aacagcggga cgccgatgaa gctcagcgcg
     3601 gcgatggtga aggcgcccat ggtccagggc atgacgcgcc cgatgcccgc cagctcgtgg
     3661 atctgggtgc ggccggtggt gcgctgcacg gcgccggcga cgaagaacat ggtgatcttg
     3721 gcgaaggcct ggttggcgat gtgcgcgatc gccgccagcg ccgccagcgg cgtcagcagc
     3781 gaggcggcca gcacgatgta ggacagctgg ctgacggtgg aataggccag ccgcagcttc
     3841 aggttgctct gcgccagcgc catgagcgat gcggcgatga tggtgaagcc ggcaaacagg
     3901 gccagcacgt cggtgtagcc gagcacgccg agcagcttga tgccgaagac gttgtagatg
     3961 acgcgcagca gcccgaacac gccggccttg accacggcca cggcatgcag cagggcactg
     4021 acgggcaccg gcgccaccat ggcggtgggc agccagccgt gcagcggcat gatcgcagcc
     4081 ttcacgccaa agccgccggc cagtgcgacg aacgtcacca gcagcatcgg acgccccgca
     4141 tccagcgaca gcactcccgg catcgacagc gtcagggtgc cggccaggtg ttgcaccagc
     4201 acgacgccga gcagcaccgc cgcgccgccg gacaaggtgt aggccaggta cttccgcccg
     4261 gccttcatgg cctcgggcgt ctcgtcgtgg atcaccagcg gataggtaca gatcgtcaag
     4321 agctcgtaga acaggaacag cgtcagcagg ttctcggcga aggccagccc cacggtggtc
     4381 gacacgcaca gcgcgaagaa gccgaaaaag cgcactcgct cgtgactacc ctgcatatag
     4441 ccgatggcgt agaccgtggt caccagccac agggtcgaag agacgagggc gaagaacatg
     4501 ccgaggccgt cggcgcggaa cgcgatgccg atgccggggg ggaagtctac gaggtcgtac
     4561 acgtagacgg tccccgcgag gctgccgggc agcatcgaga gcacgatcgc cagcttcagt
     4621 gccgaggcgc cgagcgcgaa caccgcgcgc cacaggtccc gccgcccgag catgaagatg
     4681 aggatggcgc aggccagtga caacgccggg gcaaggacta tgcggatgtc gacgacttcg
     4741 atggccctac cctccgcccg acaggcgggt gaccgccggg gcgatcagcg ccaccggcca
     4801 gcgcccgagc agcccggcca gcacgcacag catcgccagc agcagcaccg gccacaggat
     4861 cgcggcgggc gcctcgcgca ccgccagcat ctgctcgtgg atcggcgcac gaaaatagaa
     4921 ggcgttgagc atgcggccga ggtagatgaa caccagcagc gcacccagca gcaccaccac
     4981 ggcgaagccc ggccggccgg cttccacggc gccgagcgcg atgtaccact tgccgaggaa
     5041 gcccgcggtg ggcggcaggc cggcaatggc cagcacgccg atcagcaggg ccagcgaggt
     5101 ccagggcatg gcgtggccga tgccgcgcag gtcgctcagg gtgcggtagc cgctctggtg
     5161 cagcatggcg cccgccgcga ggaacagcgt ggccttgatc agggcgtgat tgaagacatg
     5221 gacggtcgcg ccggtcacgg ccagctcccc cgccagcccc agccccagca cgatgtagcc
     5281 gatgttggag atggtggagt acgccagcat cagcttgatg tcctgctgga acagggcgaa
     5341 gaacgccccg gcgaggatcg agaccgcccc gagccaggcc agcgcctcgt acaacggcgc
     5401 cagggacacc gtggcggccg ccgcaaacag cggccagacc cgcagcacgc cgatgatgcc
     5461 gagcttcacc accagcgccg aggacacggc attgatcggg ctgggcgcga tcgcgtgcgc
     5521 gtccggcagc cagacatgca gcgggaacag gccggccttg accaggaaac cgaccgtcat
     5581 gccgaccagc gccagcacga ccggcgcacg cgactcgacc tccgccagcc gcgacgcaat
     5641 gtccgccata ttcaggctgc cggtcaacgc gaacaccacg ccgatgcaca gcaggatcag
     5701 tgccgacgac acgctgccgg ccatcaggta cttgaaggcc gcgagcgccg ccgtgcgccc
     5761 gccgccgacg gccaccagcg cgtagctgga taccgcgacc agttccatga acacgaacag
     5821 gttgaacagg tcggcggtca cgacgaagcc gatcatcgcg ccgagattca ccagcagcag
     5881 gctgtagtac caggggatgc gttgcgccgg gacggcgcgc cgcacgtacg gcccggaata
     5941 caccagcacc agcaccatga tgaagctgat gaacagggcc gcggcgctga attcgtcgaa
     6001 gcgcagctcg atgccccagg gcggcggcca gccgcccatc gcataggaaa acgggccctc
     6061 ctgcgccacg cggtgcgtca gcacggccgt cacaacggtg cagaacacgg ccgtcgccag
     6121 cgcccagccg cgagccaggc cggggcggcc ggccgccagc gcgggcgtca gcgcgccggc
     6181 cagcaagggc agcacggcgg ccagcggcag ccagtgcgcc atcactccgc ccctttcagt
     6241 tcatcggcct cgatggtgcc gcagtggcgg tagatctcga tgatcaggca cagcgccaac
     6301 gccgtgatgc tcaacgccac cacgatgccg gtgagaatca gcgcatgcgg caaggggctg
     6361 gcgaagggcg ccgtggcgtc ggcgccgagg atcggcgcgg tgccgccgtc gaccatcccc
     6421 gaggtgacca cgaaggcgaa cacggccgtc tccatgatgt tcaggccgag cagcttcttg
     6481 atcaggttct gcttggcgat gatgacgtac aggccgatgc agaacagcac gacgaaggcg
     6541 gcgtagtcga tgttctgcca caggccggtc atctcagtca ccccgcatgc cgacaaacag
     6601 cccgaagatg accgccgcgc caccgatgcc gatgccgagt tcgagcccga tcatcatcag
     6661 ttcgcgcagc agggccatcc ccggatcgtc ggggaacccc agcaaggcac ccgtcagcgc
     6721 gagtccgagc aggccgacca cggcgaaggt caacggcgcc gccgcctgca gccagcgcgc
     6781 cgcgacggtc gggaccagtg gcgcgcgcgg accccgcccc atgacgacgc tgagcagcat
     6841 cagcagggcg ccgaggatga cgccgccctg gaagccgccg ccgggcgaga cgtggccctt
     6901 caggatcacg aaggcgccga acagcgcgat gagcggcgcc gtcagcctga cgacgtaggc
     6961 cacgacggga ctcagcggag cgccgggagg accgcccgga ggaacgccac ccgtcccacc
     7021 cggcgcggcg ggcgtgagca cggccattgc ggcggccagc gccgtaaaga tcaccgtcac
     7081 ctctccgaag gtgtccagcc cgcgatagtt cagcaggatg ccggtgacca ggttgtcggc
     7141 tccggcctcg cgcgcgccgt ccgcgaggta gcgggcggcc acgtgggtgt gcgccggcgc
     7201 cccaccatca cccggccgcg gcagcgcggc aacgccgatc agcatcggca cggccgcagc
     7261 ggcgatggtg agcgcaacca gcgtttttct catcgctcct tcctgcaggt gcggccgatc
     7321 gccaccagca ccaggatggt cgtcacgccg gcgccgaccg cggcctcggt catcgccacg
     7381 tcgggagcgc cgcggtgctg ccacagcagg cacatcacca ggctgtaacc ggagaagatg
     7441 atggcggccg cgaccaggtc cttgctgcgg gccacggcca gcgccatggc gatcagcagg
     7501 ccgagcatca cgagatcgaa gacctggctc atggcgcatc ccccgccgac ggctcccacg
     7561 actccaggcc ggtgccatgc gcggcgtgcg ccagggcgtg tccggcggtc gggctggaaa
     7621 tgagcaccag tgcgagcagc agcaggatct tcagcgcctc cgggtgcggc gcgacgtgta
     7681 ccgccagcgc cagcaggatc gcccccgccc cgacggtgtc gcacttcgcc acggcgtgcg
     7741 cgcgcgtgta gaagtccggc agcctgagca accccaatgt gcctgccacg aagaagccgg
     7801 cgccgaccag ggccagcagg acggtgagcg agtgcagcgc ggcgcccatc atgactgcca
     7861 gtcccccttc agccggccgg tctccagaaa gcgcgtcgcc gcgatggtga tgccgaagtt
     7921 cagcaggccg tagaccaccg ccacatcgag cagcaggctg cgttcgaagg ccagcccgag
     7981 cagcaccagc acgatcaggg ccttggtgcc gacgacattg gtggcgagaa tccggtcgac
     8041 cacggtcggg cccgcgaagg cgcgccacac gcacagcgtg gcgttgagca gcaggaagat
     8101 ggccagcccc cagaagaagg ccgtcacgac ggcgactccg gctcgaagat ccgcgccacc
     8161 tggcgctcga ggctgccgtc caggatccgt tccgccgagg cctcgtcgag gcagtgcagc
     8221 aggaaatcac cgccccgcag gtcgaccgtg agcgtgccgg gcgtcagggt cacggcctgc
     8281 gcaaaagcaa tccgggagat gtcgcgccgc aggcgggtcc ggcaggtcac cagccgcggc
     8341 cgcaccggca ggcgcggatc cagcaccact cgcgccacgt gcagcgcgga cagaaagatc
     8401 tgccaggcga aaccggggga ccggcgcagc agcaacgccg cctcgcgcat cgacagccgg
     8461 ggtgcgtcgc cggcccacag gtagcgcgtc gaccacccgc ccagcagcag cgagagcacg
     8521 aggccgaccg ccaggtccgt ccaggccagc gaggccgaga tcaccagcca gaacacgaat
     8581 acgaccatac cggccgcgat gaaccgtggc gtgctgcgtg gcccgttcgc catccctggc
     8641 ccgtccacgc gaaatgggtc gcgtgacgaa agcatgctgc gaaccccggt gcgggccgag
     8701 cccggccacc ggcgtcgtgc cgacggcctg cccggaaacc ctcaggcccc aggagtccta
     8761 tagttcaaca cggttgcgaa tgccttgacc cgcgtcaata ggtagccgcc accctcacgg
     8821 ccgcggcgcg ggtcagggcg tgtagtaggt cgcggtggca ggtccgaccg gcaggccgag
     8881 cacgaacacc cagaggaaga aaaaggccgt ccagcagatg atgagcgcca ccgaataagg
     8941 cagcatcatc gagatcagcg taccgatgcc cgccttgggc acgtagcgcg ccgcgaaggc
     9001 catgatgagt ccgaagtagc tcatcatcgg cgtgatgatg ttggtggtgg aatcgccgat
     9061 gcgataggca gcctggatga tctccgggct gtacccgagc agcatcagca tgggcacgaa
     9121 gatcggcgcc gtgaccgccc actgcgcgga ggccgagccc agcatcaggt tgacgaaggc
     9181 gcacatcagc acgaagaaca cgaacaccag tggcccggtc agcccgatcg acttgagcag
     9241 ctctgcgccc gtgaccgcgg tgaccgcccc gaggttggtc cagccgaaga atgcgacgaa
     9301 ctgggcggca aagaacacca gcaccatgta cagcccaagc gtgctcatgg cgctcgccat
     9361 ggcgttgatc acgtcgcggt cgctgcgcat ggtcccgacg gccgcgccgt aggcgatgcc
     9421 cggggcgagg aaaaatacga agatcagcgc gacgaagccg cgcaggaagg gcgagtcctt
     9481 gatcgcaccc gtttcggggt tgcgcagcac ggcaccctcg ggcaccacca gcagcgcgat
     9541 cagcccgacc atcagcagcg cggcgatgcc tgcatgtcgc aagccgcgac gctccgcatc
     9601 cgagagcgga tccatgcggt gctcgtcatc gtcgccgagc cgggcctggc cgcgatcgta
     9661 gggaccgagg gccggctcca cgatcctgag gctgatccag ctgacgacga tcaccaccag
     9721 gaaggtgctg ccgaccatga aaaaccagtt ggccgttgcg tccaccaggt agccgggcgc
     9781 gatgagtcgc gcggcctcct gggtgatgcc ggccagcagc gggtcgaccg taccgaccag
     9841 caggttggcg ctgtagccgc ccgacacgcc ggcgaaagcg gccgccaggc cggcgagcgg
     9901 atgtcgaccc agcgagtagt acaccatggc ggccagcggc acgagcacca cgtagcccat
     9961 ttccgaggcc gtgttggaaa gcactccggc gaacacgatg gtgacggtga ccatgcgcgg
    10021 gctggccctg agcaccatgg cgcgcaccat cgcagtgagc agtccggatc gctccgccac
    10081 gcccacaccc agcatcgcga ccagcacggt gcccagcggc acgaaactgg taaagttggt
    10141 gaccaggttc tgcacgatgc gccgcaagcc gtcggcgttc atgaggctga cgacccggat
    10201 catgccgtcc gcggcccggc cgtcggcgcc gacgggtcgc ggatcgggca ccgccacgcc
    10261 gagcgcgccc atcaatccgc tgaacagcac cacgccaaag gcgaacagcg cgaacagcgt
    10321 cacgggatgc ggcagcgcgt tgccgagcca ctcgaccacg tgaaggaagc gcgtgatgcg
    10381 gctcggcgcc tgcccggcct catgctcgcc cccgttcggc tccggttctc gctcgtccgc
    10441 cacagaatcn nnnnnnnnng cggccgaagg gcgccgcaag gcgcgcggcg ccgggcatcc
    10501 acacggccgc gcgtcccgaa atataatcgg cctgcagcaa attccggaga gtccatcgtg
    10561 cccgcattga tcgccctgtt gctcgcgctc gtcatcgggg cgttcttcgt gtcgcggcgc
    10621 gccggcatgc tgatgctggc cggcgcctcg gttgcggtcg cggccatgtt cgcctggacc
    10681 tggcacacgg gccccggcgt ggaattgcgc cgcgacgcca tccccgtggc cgaaatcgag
    10741 atcctcgaca cgcgccggcg cggcaacacc accgactacc tggtccgcaa cggcagccag
    10801 ggctggaccc tcaccgccct gtacaccgaa cgcattgccc gcgacgcggc cggcgaggtg
    10861 gtggaccgca ggaccttcaa gcacatcgtc gaggtgccgc cgggtgaatc cagctggcag
    10921 acgctgcgat tcttcggcct cgaaatcggt cggcaatacg agctggaagt cgtcgggacg
    10981 gaaggcgcgc ggccgcgcta gaatcgccgc catggccagg atcatggtgt tgaaacacgt
    11041 cgcggccgag ccgctcggcc tgctcgaccc gatgatccgc gcgcggggcc atcgcatccg
    11101 ctacgtgaac ttccagcgcc atccggacgc ggagccctcg ctggatcgtt accaggcgct
    11161 ggtcgtgctg ggcgggccga tgaacgtcgg cgacacggac cgccacccgc acctgctgac
    11221 ggaatgccgc ctgatcgagc aggcgctggc gcagcgcatc ccggtgctgg gtatctgcct
    11281 cggcgcgcag ctgctggcgc acgtgctggg cgcccgggtc gcgccggcgc ggcggcccga
    11341 aatcggctgg cacctcgtgc gcccgcgcct ccccgccgcc gcggatgatg cggtgctgca
    11401 cccgctggat gccgagcgcc cggtattcca gtggcacagc tacggcttcg acattccgga
    11461 cggcgcggcg cacctggcgg aaagcgacga ctgtcccggc caggccatcg gctacgacgg
    11521 ctgtgcctgg ggattccagt ttcacctcga gctcgacgag agcctgattc gccgctggct
    11581 cggctcgccg gggtatctcc gcgaactggc cgcctcgggc ctcgaccgga cgccggacga
    11641 gattctcgac gacacgcggc accacctcgc cgccacgcgc gtgctcgcgg cgcgcgtgtt
    11701 cggcaactgg ctcgacctgc tcggccagaa cggggggcgc cggctgctgc cgtcgcgctg
    11761 accttgcccc gcgcgacacg ccgcactgaa atgccgatca ccggcgtccc cgactcgccg
    11821 cgcgagttcc tcggcgtatt ccgctacagc cggcgcgcgc tgcagctggt ctggaccacc
    11881 agccgccggc tcaccgtgat cctggcggtc gtcaccgtgg tggccggcgt gctgccggcc
    11941 gccatcgcct tcgtcggcgc gctgatcatc gacgcggtgg tcgctgcggc gcgcctctac
    12001 caggagagcg gcgcggcgca gttgcgcgag gtgatgggcc tggtcgccgc cgagggcctg
    12061 ctggtggcgg cgctggccgg ggcacagcgc gccttgtccg cctcccagtc gctgctgcgc
    12121 gcccaactcg gccagcgggt caatgtcatg atcctcgaga aggccctgca gctggagctg
    12181 tctcagttcg aggactcgga gttctacgac aagctcaccc gagcccggcg cgaggcctcc
    12241 agccggccgc tgtcgctggt gatcaggacc ttcgggctgg cccagaacgc cgtgtcgctg
    12301 gtcagctacg gcgtgctgct ggcgcagttt tccgcctggg ccgtgctggt gctggtgctc
    12361 gccggcctgc ctgccttcct cgccgaggcc aagttctcgg gcgacgcctt ccggctgttc
    12421 cgctggcgct caccggaaac ccgcatgcag atgtacctcg agaccgtgct ggcgcgcgag
    12481 gaccatgcca aggaagtgca gctgttcggg ctcgggccgc ggctgctcga ccgctaccgc
    12541 cagatcttcc agacgctcta ccgcgaggac cgcaacctga ccctgcggcg tgacggctgg
    12601 ggtttcgtac tcggcctggt cggcacgctg acgctgtatg gcgcctacgc ctgggtggcg
    12661 ctcaccgctg tgctcggacg catcaccctc ggccagatga ccatgtacgt gatgctgttc
    12721 cgccagggcc agtcggcggt ttcggccagc ctctcggcga tcggcggcat gtacgaggac
    12781 aacctctacc tgtcgaccct ctacgaattc ctcgagaccc ccgtgcgcgc acgcggggac
    12841 accctggcgg cgggcccgca accgggcgac ggcatccgct tcgagggtgt cggcttcacc
    12901 tatccgggca ccgaggcgcc ggcgctgcag gacatcgacc tgcacatccg gccaggccag
    12961 tcgctggcgc tggtcggcga gaacggctcc ggcaagacca ccctgatcaa gctgctcgcc
    13021 cgcctgtaca cgcccaccgc cggccggatc ctgctcgacg ggctcgatct cgagcgctgg
    13081 gacgagcatg cgctgcgccg gcgcatcggc gtgattttcc aggacttcgc ccgctaccag
    13141 ctcaaggtcg gcgagaacat cggcgccggc gacgagccgc gcttcgagga ccgggcagga
    13201 tggttggctg cggccgagca gggtatggcg ctgccgttca tcgaatccct gccggagcgc
    13261 ttcgagacgc agctgggcaa gtggttcaag caggggcgcg agctgtccgg cggccagtgg
    13321 cagaagatcg cgctcgcacg agccttcatg cgatccgagg ccgacatcct ggtgctcgac
    13381 gagcccaccg cagcgatgga cgccgcggcc gaggccacca tcttcgaaca cttccgcaac
    13441 ctgacccgcg accgcatcgc gatcctgatc tcgcaccgct tctcgaccgt gcgcatggcc
    13501 gaccagatcg tggtcatcga ggggggacgg atcatcgagc gcggcagcca cgatgagctc
    13561 atggcgctgg acgggctgta cgcccggctg ttctcgctgc aggcgcgcgg ctaccgctag
    13621 cggcccccgt gcgggctcac aaggcgcgca tgcgctcggc cgcgacccgc acctggttgc
    13681 gcccgccgga tttggcctcg tacagagccc ggtccgcgag accgagcagc tcccggcgac
    13741 tcacgccctc gcgcatggtg gcgacgccgg cgctgagggt gacgcgcacc cccggcacca
    13801 gcagctccca ttcggtgctc gccaccgtct cgcggatctt ctcgcaggcc tcgcgcgccg
    13861 ccaccatgcc ggcctgcggg aagcacagca ggaattcctc gccgccgtag cgtgacacca
    13921 ggtcggtgtc gcggcagcgt tccctgagca gctgggccac cagtcgcagc acctcatcac
    13981 cggtgtcgtg cgaatggtcg tcgttgatgc gcttgaagtc gtccaggtcc accagcgcga
    14041 tggcgaaatc gcggccatag cgctcgaact cgttgatccg gtcttccagg tactggtcgg
    14101 cgtgccgccg gttgtgcagc cccgtgaggg gatcgtggct ggccatccgc tccagctcga
    14161 gactcttctg ctgcagcttg cgggtgcgct ccgcgaccag cgcttccagc cgggtacgga
    14221 tgtgcgccag ctgcatcagc gagaacatgc cgagggtcat caccagcagc agcaggacga
    14281 ccgcggacag ctccatgcga ttgatgatca gcgccaccag gattccgccc agccccgcgc
    14341 cggactcgac cacgaaggcg aaggggctga agtccgtcgg cacgcggcgc tcctcgagcg
    14401 tcatcagcac gcgcatgccg agatcgttca atgcctgcat gctgaggagc aacagcagca
    14461 gcgtggcgac gtcccgccag cccagcgcca gcagcggcac cgggccaccg agcttctggt
    14521 acagcaggcc gcagaccagg atcatcagcg acatcaggcc ggcattgtgc agcgccgccg
    14581 tcagcacctc gatgaaggga cggccccgcc gcaggcgctg ccacgggtac agcagcgatg
    14641 ccgtgccgtt gatccaggcc gcggcgaccg gccccagcac caggatggcg gcgacctgcg
    14701 cgatccggtc gaaggagaca tagcccacgc gcggatgcgt gtaacccatg aaaatcgtga
    14761 acagcgcata gacgaggaag aacgccacca ggccgggccc cgcatccggc ggcaagagac
    14821 cctggtccgc gatcgccacc gagagggcgg cgagcgccgc gacgaacagc gccagggagt
    14881 agatgaactc gggccggcgc aaggccgggc gagccgcggc gccgggcggg ccgccagtcg
    14941 tgctgtcctg ccgttcgttc atgctgcgtc ctcagcccgc cagcagacgg actgccaaag
    15001 gtagcagaaa agcggtcagc agcccggcca tgccgaggcc cagcccggcg aaggcgccgg
    15061 cgcgcaggcc gatggtcagc gcgtgggcgg taccgaagcc gtgcgcggca atgccgagcg
    15121 cgaagccctg cgcgcgctcg tcgcggatcc ccgccagccg gaacaacggc cccgccgcca
    15181 tcgcgccgac ggcgccggtg agcagcacga tgccggccgc cagcgacggc aggccgccga
    15241 tctgctcggc gatgcccatc gcgatcggcg tggtcacgga cttcggcgcg atggacaaca
    15301 gggtctggtc ccgccccccg gccagccagg cgagccccac ggcgctcgac accgccaccg
    15361 tgacgccgag caggcaggcg aacagcagcg gccacagcag gcgccggatc tggcgcaggt
    15421 tgtcgtacag cggaatggcg agcgcgacgg tcgccggccc gagcaggaaa tgaatgaact
    15481 gcgcgccctc gaaataggtg tcgtaggagg tgccggtgag cagcagcagg ccgatcagca
    15541 ccagcattgc aatgagcacc gggtgcgcca ggggatgacc cttgaggcga tgattcagcc
    15601 aggtgctggc gacgaagatc gccagcgtga cggtcagcca caagagcggc tcggcggcca
    15661 gatagaccca gatttcgccg agctcagtca cttgcgtcct ccaccggctc cgcgtggcgc
    15721 gcgatcagcc agccgaccag cagcatggtc agcagcgtgc tgccgacgat ggcgagcagg
    15781 atcgggaacc agtcggtacg caggcgcgcg ccgtgggtga tgaggccgac gccggccggc
    15841 acgaagaaca gcggcaggta ggtgagcagc ccggtgccgg cgcgccgcac gcccgcggcg
    15901 gggccgccgg ctgcgaccag caggagcagc agcaggacca tgccggcgac cggcccggga
    15961 aacggtacgc cgagcagccg caccagggcc tcgccggcca gctggcagat gaagatgagg
    16021 gtcagacctt gcagcaccgg ccaagtatgc cggagccgcc gtgctcgtgg gagcggcggc
    16081 tcaggctcgc gggtggaccg cgaggcggga acgacatggg tccccgtcgc tcaggaaatc
    16141 tttattcgct ccgtggaccc atgccatccc cgcctcgctg cggcgttgcc gggggaagcg
    16201 gacacacttc caagaaccca gttcgctgtc acagtggacg aagccacttc aagatcacga
    16261 ttccaacaag aaggccactg ataacgaagc cagcggcaat ccagttgtat tggtcgtcca
    16321 gcctaaggtg cgaggagatc tgccccatgg cagcagcgat ccccagacaa atacccatac
    16381 acgatatgta aaccacccat ccacgcttgg tggtcgagcc gcgtgcatcc ttcggtatgg
    16441 cgaagggaac ggcggcgacg gcaccaatca gcaggccaag gattagaaac aatacaacat
    16501 gagcggtcat ttcccgcaac cacaggcgac tgacgtttca gtcgatccaa tgccgccgaa
    16561 attgctcggc agcgtcatct gctggaggcc gtttgcgccg acagcgtgca ccgagccaac
    16621 agccaaactt gtagatatgg cttggtctgc cgtcgcacca cttcttaaca tccgcgggcc
    16681 gtgtgtgaat cctgctgcgc gcccccaatt tccaccgacc gcgccaacca tactttgtac
    16741 aaggacttgt gctccggaaa actctcggga gccgagaagt tgtcctatag cgttcccagt
    16801 cgcgcccgcc aatccatttc tgacgatgtt gctgacgacc gtgcctccaa cggacaagtt
    16861 gcccgacgct ccgcccacaa ccatccctgc ggccgtcgcc aaagcgattg aacctggagt
    16921 cgaacatgga tcattgagcg ctgcgatgag agccgaggtt cctccgacca caacgccaaa
    16981 accaaccggc actaataccg cgtactcgcc ggtgggatcg ataaaccgca ccggattccc
    17041 ccccacatac ccataagtat tcagcccgcc cgccaacccg atcggatcgg actgggtgta
    17101 gcggccgatg gacccgtcat acgtccggaa ccagttctgc cagctcgccc cctcggcatc
    17161 gtaatactgc cccgggaacc cgaggttgta
//
LOCUS       DAOMPG010000021        17116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1475_length_17116_cov_4.295645, whole genome shotgun sequence.
ACCESSION   DAOMPG010000021 DAOMPG010000000
VERSION     DAOMPG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 17116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_17116_cov_4.295645"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1324)
                     /gene="aceA"
                     /locus_tag="PLS34_02060"
     CDS             complement(41..1324)
                     /gene="aceA"
                     /locus_tag="PLS34_02060"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRP86290.1"
                     /translation="MTRLSAEALKKDWNENPRWKGVTRPYTAEDVVRLRGTVDIEYSL
                     ARQGAEKLWKYLHEKPFVNALGALTGNQAMQQVKAGLNAIYLSGWQVAGDANLAGEMY
                     PDQSLYPANSVPAVVRKINRTLLRADQIHHAEGNDKIDWLQPIVADAEAGFGGVLNAF
                     ELMKDMIDAGAAGVHFEDQLSSAKKCGHMGGKVLVPTAEAVSKLAAARLAADVCGVPT
                     LVVARTDADAANLLTSDIDERDAPFVTGERTSEGFFRVKAGLDQAIARGLAYAPLADL
                     VWCETSVPDLGMAKKFAEAMHKEFPGQMLAYNCSPSFNWKRHLDDATIAKFQKELAAM
                     GYKFQFITLAGFHALNYSMFTLAKGYKESQMKAYVELQEAEFASEKDGYTATKHQREV
                     GAGYFDAVTQVVTAGQSSLSALAGSTEEEQFEAAH"
     gene            complement(1360..2958)
                     /gene="aceB"
                     /locus_tag="PLS34_02065"
     CDS             complement(1360..2958)
                     /gene="aceB"
                     /locus_tag="PLS34_02065"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRP86291.1"
                     /translation="MTSTRTDAEYRILGPIREGYEAILSPEAMGFVAALAKRFGPRVD
                     ELLERRKARQAEIDAGRMPDFLPETAAVRAGDWRVGAIPADLQDRRVEITGPVDRKMI
                     INALNSGAKVFMADFEDSMTPSWDNLVLGQIALRDAVNRTISFTSPEGKRYRLNEQVA
                     TLFVRPRGWHLFEKHVEVNGRQVPGGLFDFGLYLFHNAQGTAAIGTGPYFYLPKLESH
                     LEARLWAEVFRFAEDTLGLARGTIKCTVLIETILAAFEIDEILYELREFIVGLNCGRW
                     DYIFSFIKKFQNFPEFTLPDRQQVTMATHFLRSYSQLVIKTCHRRGALAIGGMAAQIP
                     IKGDPEANEAALAKVRADKEREAGDGHDGTWVAHPGLVPVAMEIFDKHMPRPNQLERL
                     REDVQVSAADLLKVPTGEITEAGLRSNISVGIQYMAAWLGGNGCVPLHNLMEDAATAE
                     IARAQVWQWIRHPQGVLSDGRKVTYALYAQIRDEELAKIREQVGEEAFASGNYREAAG
                     LLDRITGEQHCMDFLTLVAYEAID"
     gene            complement(3139..>3616)
                     /locus_tag="PLS34_02070"
     CDS             complement(3139..>3616)
                     /locus_tag="PLS34_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86292.1"
                     /translation="GPTQRVEKDHRRTLQELDAWARAYPEGREDSGYQYARIWSLLWL
                     GEDAQANALLGEHFIDSNDPDRVANYLYLAFRSPTDVLLAEAEPRARQAIVRFPEQAA
                     RFNAAHGRLLRRLGRLQEAEAAFGRAVAGVSADNPSHGTYLGQLEFVRKELAAAAD"
     assembly_gap    3617..3657
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3658..3859)
                     /locus_tag="PLS34_02075"
     CDS             complement(<3658..3859)
                     /locus_tag="PLS34_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86293.1"
                     /translation="MAAIVGYPDDYVARLEAAVAGHDAIASAQAQLRDRNDVESLEAL
                     AEAFLAAGDAPAARRVYQSLLWR"
     gene            complement(3941..4243)
                     /locus_tag="PLS34_02080"
     CDS             complement(3941..4243)
                     /locus_tag="PLS34_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP86294.1"
                     /translation="MHRIAVLLALIALSAAPAAAAVRFTDWSGDSLPALVEEAAASDR
                     MVMVVITQPDWCPGCIALDRELLRNPAAEEFAEFTRDWVILEMLGYDQPDAGVLAE"
     gene            complement(4318..5961)
                     /locus_tag="PLS34_02085"
     CDS             complement(4318..5961)
                     /locus_tag="PLS34_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HRP86295.1"
                     /translation="MKRQVIPLALACAGVLLQAVPAGVAASEPTAPINEAALHRHIAA
                     LSADEMEGRWPGSKGEALTLEYLATEFARAGARPGNGDSFLQPVPLVSWQLDPAPSIT
                     IRGGEFSAELAYREEMVAVTRRLVDAVAVKDSPVVFAGYGVVAPEYGWNDYEGLDARG
                     KTVVVLVNDPGFASGDPELFNGRAMTYYGRWTYKYEEAARQGAEALLIVHETEPASYG
                     WEVIGRGKDEHFGLESADNQSRAAVEGWLSAEAAEMLFAAAGEDFATLKAAALKPGFR
                     ARPLGDLRADMRVSNTIGRTASNNVVALVPGAERPEEYVVFTAHWDHLGSEGEEIYNG
                     AVDNASGTAALLLLAEAYARQDPPPARSILFVAVTAEEQGLLGSAWYAANPLVPLEQT
                     AAVINVDVLNFIGPTRDISVVGYGASELDAFVDAAAAAHGRKVVPDQRPEAGTYYRSD
                     HFNFAKRGVPALYPKMGLDLVDGGIERGRELAGAYNAERYHKTTDEYSPDQDWSGAVR
                     DLKLYFDIGLGIANSSDWPNWMPGNEFRAVRDAQRPADR"
     gene            complement(6001..7353)
                     /locus_tag="PLS34_02090"
     CDS             complement(6001..7353)
                     /locus_tag="PLS34_02090"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRP86296.1"
                     /translation="MATNPEPREWHPASWQSKVAGQQPMYPDTDAVASVIARLSALPP
                     LVVSWEVEALQAQIARAQRGEAFLLQGGDCAESFDDCNSDQIARKLKILLQVSVVLLH
                     SLKKPVIRVGRMAGQYAKPRSADHETREGTSLPSYRGDLVNRNAFTLADRTPDPELML
                     TGYNRAALTLNFVRALAEGGFADLHHPEYWNIGFAQSTPFEQAYKRIVKSITDSMDFF
                     EFISGQPVHRTRRIDFFTSHEGLHLLYEQAQTRFIARNNRWYNLSTHFPWIGMRTAQV
                     DGAHVEYFRGIANPIAVKVGTGMSIEWLQSLIETLNPDNEPGRLALIHRFGAARIAEH
                     LPPMIEAVRAAGATVLWVCDPMHGNTESTSSGIKTRSFDRILSEVEQAFRIHEEMGSY
                     LGGVHFELTGDDVTECIGGARGLTEADLNRAYLSQVDPRLNYEQALELAMRIAELRGR
                     "
     gene            7533..8696
                     /locus_tag="PLS34_02095"
     CDS             7533..8696
                     /locus_tag="PLS34_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRP86297.1"
                     /translation="MHRILVLGAGKIGALISGLLAESGDYRVDLADVDAAAAQAVVKA
                     HGLDAVRAFGLDATDRAALDAHLDAHPADAVISGLPYFCNVPVAEACRARGMHYFDLT
                     EDVAVTEQVRKIAAGADTAFAPQCGLAPGFISIAANELAKHFAELRTVKLRVGALPQH
                     PNNVLKYSLTWSTDGLINEYGNMCEAVENGETIKVLPLEGLEEIEIDGTKYEAFNTSG
                     GLGSLADSWQGRVRRMNYKTMRYPGHCSQMRLLMNGLKLNNDRDTLKRILENAVPRTL
                     QDVVIVYVSVVGKQDGELREENYVNKIYPQVIAGRLWSAIQVTTAAGVCAVADLVLNN
                     PGAHRGFVTQESFQLREVLDNRFGRYYAHGGTYEVSARMIATGEVGHQRAKGA"
     gene            8698..10218
                     /locus_tag="PLS34_02100"
     CDS             8698..10218
                     /locus_tag="PLS34_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP86298.1"
                     /translation="MQDILQRLGLAEENSGAWAGTRELPGSGPVIESFNPATGQRIAS
                     VRSASEDEYEEIIGSAREAFHLWRAMPAPHRGEAVRLCAEALRRHKDALGSLVSLEMG
                     KIKAEGDGEVQEMIDIADFAVGQSRMLYGRTMHSERPGHRMYEQWHPLGVVGIISAFN
                     FPVAVWSWNALIAAVCGNVCVWKPSPKTPLCAIAVQKICNEALADGGFPPIFNVFVEA
                     DVAMAEAFVDDERVDLVSFTGSTPVGRRVAQRVAARLGRSLLELGGNNALIVDEKANL
                     ELAVPAIVFGAVGTAGQRCTTTRRVLVHEKLFDELAHRLMHAYSQVKVGDPLDPKTLM
                     GPLIDADAVLRYEAAIEKLIARGGRVLTGGHVIDRPGHFVQPTIVKASNEWDVVQEET
                     FAPILYLIPFHHFDEAVALNNAVRQGLSSAIFTDDLQAAERFLSATGSDCGIANVNIG
                     TSGAEIGGAFGGEKDTGGGREAGSDSWQAYMRRQTNTINYSHELPLAQGIKFDLEG"
     gene            complement(10249..10743)
                     /locus_tag="PLS34_02105"
     CDS             complement(10249..10743)
                     /locus_tag="PLS34_02105"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRP86299.1"
                     /translation="MDIVFLTTGGTIDKVYFDAMSRFEVGETVVGKILREGGVTFEFE
                     VIPLMRKDSLEMVDADRAAIHAAAAAREERRIIVTHGTDTMVETARALSGIEGKTIVL
                     TGALSPARFQASDAVFNIGMAVAAVQTCPPGVWIAMSGHVFACDQVRKNRGLNRFEPI
                     EPAG"
     gene            10845..12278
                     /locus_tag="PLS34_02110"
     CDS             10845..12278
                     /locus_tag="PLS34_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019017412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRP86300.1"
                     /translation="MKTLRALFLSLACLGLLAAFGQAASQGGGQDAGGRALLLELDGA
                     IGPATSDYIVRGIRKAAEEQAQLVIIQMDTPGGLDTAMRDIIKAILSSPVPVVTWVAP
                     SGSRAASAGTYILYASHVAAMAPATNLGAATPVPVGGGQPAPPAEEPAGRDRAEDRDA
                     DKADEQAAEADDRREPAPMRGGPEAKAINDAVAYIRGLAERRGRNADWAERAVRESAS
                     LTAERAVEQNVIDLVAADMGELLRKIDGRKVSTESGDVVIQGEGLAVERVEPDWRTEL
                     LAVITSPTVAYMLLLAGIYGLIFEGYNPGAILPGVVGAICILLALYAFQVLPVNYAGL
                     ALIVLGILLMIGEVFMPSFGALGLGGIVAFVVGSIILMDTDVPGFGIPIVMIGSVALL
                     GGSAVMAIIWFAVRARNRPIVSGREDMIGAAAIAVDDFAGRGHVRVHGERWNAESAVP
                     VHSGQPLRVTDMHGLVLKVEPDESKEN"
     gene            12280..13068
                     /locus_tag="PLS34_02115"
     CDS             12280..13068
                     /locus_tag="PLS34_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRP86301.1"
                     /translation="MLGYFGTLLLGGVLLVLLSAIKILKEYERGVIFQLGRFYKVKGP
                     GLIIVIPVIQQMVKVDLRIVVMDVPSQDVISQDNVSVKVNAVVYFRIVNPERAVIQVE
                     NVYEATSQLAQTTLRSVLGKHELDEMLSERDKLNTDIQNILDEHTDNWGIKVTNVEIK
                     HIDLNESMVRAIAQQAEAERARRSKVIHAEGEMQASARLVEAAQQLATQPQAMQLRYL
                     QTLSQIANEKTNTIVFPLPLDLIGPLMERLGQPGATATRDDQSR"
     gene            complement(13076..14017)
                     /locus_tag="PLS34_02120"
     CDS             complement(13076..14017)
                     /locus_tag="PLS34_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRP86302.1"
                     /translation="MRIGLVVDASCDLPDSVLEANGIAILPSKLELDGRSWLDERDPE
                     QTLMLYRRYIGDRAVAARSSACGASEICDIFLRDLVLDFDRVLVLTAGADFSDMFARA
                     TEASYAILQRYRERRQEEGQGGGFSVRVLDSGSICAGEAALLCRALQLLEEGRDGFEA
                     VRRTLREEVARTTCLLVPGDTWYLRRRGLDGRGRGIGRVDHALAIISDVKPVTEVQGG
                     RRRIVARARGFRAACAVALERATRAIGQGLGGGAVTLSFGGDPRVIRQMPAYQALESR
                     AATARLDLHFAVMSATMGARLGPGALSVAWIPASPAG"
     gene            complement(14073..>17116)
                     /gene="hrpA"
                     /locus_tag="PLS34_02125"
     CDS             complement(14073..>17116)
                     /gene="hrpA"
                     /locus_tag="PLS34_02125"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HRP86303.1"
                     /translation="EGDILVFLPGEREIRDTAEALRKHHPPGVEVLPLYGRLSATDQD
                     RVFRRAGRRRIVLATNVAETSLTVPGIRYVVDSGLARISRYSYRSKIQRLQVEPISQA
                     SAWQRRGRCGREAPGVCIRLYDEADHDARPEYTDPEIRRTNLASVILQMETLGLGDIA
                     EFPFLDPPDRRFINDGYRLLRELGAVDEQRVVTDLGRRLARLPVDPRLGRMLLAAGPA
                     GCLKEILVIAAGLSIRDPRERPADAAGSADEKHALFQDPRSDFLSLVKLWEAWQERRR
                     HDSGSAQRRWCRDHFLSFLRLREWQDVHRQLVEGVHQLRLRQNELPADYAAVHRAILS
                     GMLSHIGQLQEKGEYQGPRNLRFRLFPDSALAARPPRWVTVAALVETGRVWGRMAAAV
                     EPGWIESAGAHLVRRSWSDPHWQAERGFVAAREQVSLFGLVLAANRRVDFARVDAAGA
                     REIFLRDALAAGTLRTRGRFLAANRRLLREVQLLEAKLRRRELLAGDAAIAAFYAAHV
                     PPGICSTPAFERWRRKAEKAQPELLFMALDDLLTGAALPDPEAYPDTLEVGGNRLPLR
                     YRFEPAAEDDGVTVEVPRHLLPLLKEERVEWLVPGWLEEKVVAMLRALPKSARRRIVP
                     IPEYARACLALLRPGQGRLRAALAAALQRTAGLQVEPETWQRLELEPWLRMRIEVRGD
                     DGAPLAASRDLAALERAHSPGAGPVSAPAAAGWSGQGLKAWPECAMPATVEVTHGALR
                     QALFPALQDDGNTVSCRLFSSGAAAARVHRRGVLRLFMLALAQQHRALLQQARTDRTL
                     VLRGRDLAEGRDLAEDIVSAAFAAVFLPGGKDVPRTVDAFRERLDGGRADIMPEGERM
                     LALVRDILANRDACLARLADGLTGPGASDAAADIAAQLGALVRPGFLLAAPPERLRQV
                     PRYLQAVMLRMERLALGKGEARQVLDLAVQRRRLREGEATDWSTPAAAEAFEAFRWMV
                     EEYRVQLFAQQLGVNEKVSHARLEAQWQKVRELQAGLRPAN"
BASE COUNT         2629 a         6041 c         5780 g         2625 t
ORIGIN      
        1 gaccccggtt ttttgccgga aaccggtgtc tgaccccgtt ttagtgcgcg gcctcgaact
       61 gctcttcttc ggtcgagcct gccagggcgc tgagcgagga ctggccggcg gtgaccacct
      121 gggtcacggc gtcgaagtag ccggcgccga cctcgcgctg gtgcttggtg gcggtgtagc
      181 cgtccttctc gctggcgaac tccgcttcct gcagctccac gtaggccttc atctggcttt
      241 ccttgtagcc cttggccagc gtgaacatgc tgtagttcag ggcgtggaag ccggccaggg
      301 tgatgaactg gaacttgtag cccatcgccg ccagctcctt ctggaacttg gcgatggtgg
      361 cgtcgtcgag atggcgcttc cagttgaacg acggcgagca gttgtaggcc agcatctggc
      421 ccgggaattc cttgtgcatc gcctcggcga acttcttcgc catgccgaga tcgggcaccg
      481 aggtctcgca ccacacgaga tcggccagcg gcgcgtaggc cagcccgcgg gcgatcgcct
      541 ggtcgagccc ggccttgacg cggaagaagc cttccgaggt gcgctcgccg gtcacgaacg
      601 gcgcgtcgcg ctcgtcgatg tccgaggtca agaggttggc cgcatccgca tccgtgcgcg
      661 ccaccaccag cgtcggcacg ccgcacacgt cggccgccag ccgcgccgcc gccagcttgc
      721 tcaccgcctc ggcggtcggc accagcacct tgccgcccat gtggccgcac ttcttggccg
      781 aggacagctg gtcctcgaag tgcacgcctg cggcgccggc gtcgatcatg tccttcatca
      841 gctcgaaggc gttcagcacg ccgccgaagc cggcctcggc gtccgccacg atgggctgca
      901 gccagtcgat cttgtcgttg ccctcggcgt gatggatctg gtcggcgcgc agcagcgtgc
      961 ggttgatctt gcggaccacc gccggcaccg agttggccgg gtacagcgac tggtcgggat
     1021 acatctctcc cgccaggttg gcgtcgccgg ccacctgcca gcccgaaaga taaatggcgt
     1081 tcagcccggc cttgacctgc tgcatcgcct ggttgccggt cagcgcgccc agcgcgttga
     1141 cgaagggctt ctcatgcagg tacttccaga gcttctccgc gccctggcgc gcgagcgagt
     1201 actcgatgtc caccgtgccg cgaagtcgca ccacgtcctc ggcggtgtac ggacgggtca
     1261 cgcccttcca gcgggggttt tcgttccagt ccttcttgag tgcttcagcc gacaggcgtg
     1321 tcatcgcgcg ttcctctgtg gttgtgtccc cgaaatccct cagtcgatgg cctcgtaggc
     1381 caccagcgtc aggaagtcca tgcagtgctg ctcgccggtg atccggtcga gcaggccggc
     1441 cgcctcgcgg tagttgccag aggcgaaggc ctcctcgccc acctgttccc tgattttggc
     1501 cagttcctcg tctctgatct gtgcgtacaa ggcgtaagtc accttgcggc cgtcgctgag
     1561 cacgccctgc gggtggcgga tccactgcca cacctgggcg cgcgcgatct cggcggtcgc
     1621 ggcgtcctcc atgaggttgt gcagcggcac gcagccgttg ccgcccagcc aggccgccat
     1681 gtactggatg ccgaccgaga tgttgctgcg cagccccgcc tcggtgatct cgcccgtggg
     1741 caccttcaaa aggtcggccg cgctcacctg gacgtcctcg cgcagccgct cgagctggtt
     1801 gggccgcggc atgtgcttgt cgaagatctc catcgccacc ggcacgagcc ccggatgcgc
     1861 cacccaggtg ccgtcatggc cgtcgccggc ctcgcgttcc ttgtcggccc gcaccttggc
     1921 gagcgccgcc tcgttggcct ccgggtcgcc cttgatgggg atctgcgcgg ccatgccgcc
     1981 gatcgccagc gcaccgcggc ggtgacaggt cttgatcacc agctgcgagt aggagcgcag
     2041 gaaatgggtc gccatcgtga cctgctgccg atctggaagt gtaaactcgg gaaagttctg
     2101 gaactttttg ataaagctga atatgtagtc ccagcgaccg cagttcaggc cgacgatgaa
     2161 ctcgcgcagc tcgtagagga tctcgtcgat ctcgaaggcc gccaggatgg tctcgatcag
     2221 caccgtgcac ttgatggtgc cgcgcgcgag gcccagcgtg tcctcggcga agcggaacac
     2281 ctccgcccac agccgcgcct cgaggtggct ttccagcttc ggcaggtaaa aatacggccc
     2341 cgtgccgatc gccgcggtgc cctgggcgtt gtggaacagg tacaggccga aatcgaacag
     2401 cccgccgggc acttggcgcc cgttcacctc gacatgcttc tcgaacaggt gccagccgcg
     2461 cggccgtacg aacagcgtgg cgacctgctc gttcaggcgg taccgcttgc cctccggcga
     2521 ggtgaagctg atggtgcggt tgacggcgtc gcgcagcgcg atctggccga gcaccaggtt
     2581 gtcccagctc ggcgtcatcg agtcctcgaa gtccgccatg aacaccttcg cgccggagtt
     2641 cagcgcgttg atgatcatct tgcggtccac cgggccggtg atctcgacgc ggcggtcctg
     2701 caggtcggcg gggatcgcgc cgacacgcca gtcaccggcg cgcaccgcgg cggtctcggg
     2761 caggaaatcc ggcatccggc cggcgtcgat ctccgcctgg cgggccttgc ggcgctccag
     2821 cagctcgtcc acgcggggac cgaagcgctt cgccagcgcg gcgacgaagc ccatcgcctc
     2881 tggcgacagg atggcctcgt atccctcgcg gatcgggcca agaatgcggt attcagcgtc
     2941 cgtgcgggtg ctggtcatgg catgccttca cgttgcgggg ccaggtcgtg caccaccgcg
     3001 gggccgctgc aagcaggccc gcgccacccg gccgggggcc tgcggcggtg gttttctgca
     3061 ccgcgtcata ataagtccac gtcgcactca atgcaaaacc ggcgggccgg aattgccttg
     3121 tttcggcggt gggagcaact agtcggcggc ggccgccagc tccttgcgga cgaactccag
     3181 ctggcccagg taggtgccgt ggctcgggtt gtctgccgac acgcccgcca ccgcccggcc
     3241 gaaggccgcc tcagcctcct gcaggcgccc aagccggcgc aacagccggc cgtgcgccgc
     3301 gttgaaacgg gcggcctgct cggggaatcg cacgatggcc tgccgtgccc tgggctcggc
     3361 ttccgccagc aggacgtcgg tgggactgcg gaaggcaagg tacaggtaat tcgccacccg
     3421 gtccgggtcg ttgctgtcga tgaagtgttc gccgagcagc gcgttcgcct gcgcatcctc
     3481 gcccagccac agcagcgacc agatgcgcgc gtactggtag cccgagtcct cccgcccttc
     3541 cgggtacgcc cgcgcccagg catccagttc ctgcagggtg cggcgatggt ccttctcgac
     3601 gcgctgggtc gggccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     3661 gccacagcag cgactggtac acgcgtcgcg cggccggcgc gtcgccagcg gcgaggaacg
     3721 cctccgccag cgcctcgagc gactcgacgt cgttgcggtc gcgcagctgc gcctgcgcag
     3781 atgcgatggc gtcgtgtccc gccaccgcgg cttcgagccg cgcgacgtag tcgtccgggt
     3841 agccgacgat ggccgccacc tggcgcgcct cgcccagccg ctgttgcccg gcgccgggct
     3901 tgagcagcag cgtggtcggc gtgcctagga aactgagccc ctattcggcg aggacgccgg
     3961 catccggctg gtcgtaaccc agcatctcca ggatcaccca gtcgcgggtg aactcggcga
     4021 attcctccgc ggccgggttg cgcagcagct cccggtcgag cgcgatgcag cccgggcacc
     4081 agtccggctg cgtgatgacc accatcacca tccggtcgga agcggccgcc tcctcgacca
     4141 gcgcgggcag gctgtcgccg gaccagtcgg tgaaacgcac ggcggccgcg gccggagctg
     4201 cggacagggc aatcagcgcg agcaggacgg cgatgcgatg caattcactt actccaggca
     4261 acacggtcga cagattctgc ttgcgacacc cgccgcgtgc cgtggttccg cgcggtgcta
     4321 tcggtccgcc gggcgctggg cgtcgcgcac ggcgcggaac tcgttgcccg gcatccagtt
     4381 cggccagtcg ctcgagttgg cgatgccgag cccgatgtcg aagtacagct tgaggtcccg
     4441 gacggcaccg ctccagtcct ggtccggtga atactcgtcg gtggtcttgt gatagcgctc
     4501 ggcgttgtag gcgcccgcca gctcccggcc gcgctcgatg ccgccgtcga cgaggtccag
     4561 gcccatcttt ggatacagcg ccggcacccc gcgcttggca aagttgaagt ggtccgagcg
     4621 atagtaggtg ccggcctcgg gacgctggtc gggcaccacc ttcctgccgt gcgcggcggc
     4681 cgcggcgtcc acgaaggcgt ccagctctga ggccccgtag ccgaccaccg agatgtcccg
     4741 cgtcgggccg atgaaattga gtacgtcgac gttgatgacg gccgcggtct gctccagcgg
     4801 caccagcggg ttggcggcat accaggcgga tcccagcagc ccctgctcct cggccgtgac
     4861 cgcgacgaac aggatgctgc gagccggcgg tggatcctgc cgggcataag cctccgccag
     4921 cagcagcaac gccgcggtgc cggaggcatt gtccaccgcg ccgttgtaga tttcttcgcc
     4981 ctcgctgccg agatggtccc agtgcgccgt gaagacgacg tactcctcgg gacgctccgc
     5041 gcccggcacc agcgccacga cgttgttcga ggccgtgcgg ccgatggtgt tgctgacccg
     5101 catgtccgcg cgcaggtcgc cgagcggccg cgcccggaag ccgggcttca gcgccgccgc
     5161 cttgagcgtg gcgaagtcct cgcccgccgc cgcaaacagc atttcggctg cctccgcgct
     5221 gagccagccc tcgaccgctg cgcgcgactg gttgtcggcg ctttccaggc cgaaatgctc
     5281 atccttgccg cggccgatca cttcccagcc atagctcgcg ggctcggtct cgtggacgat
     5341 cagcagcgcc tcggcgccct ggcgcgcggc ctcctcgtac ttgtaggtcc agcggccgta
     5401 gtaggtcatc gcccggccgt tgaacagctc ggggtcgccg ctggcaaagc cgggatcgtt
     5461 gaccagcacc accacagtct tgccgcgtgc atccagccct tcatagtcgt tccagccgta
     5521 ttcgggcgcg accacgccgt agccggcgaa caccaccggg ctgtccttga cggcgaccgc
     5581 gtccaccagc cgccgcgtca ccgccaccat ttcttcgcgg taggccagct cggccgaaaa
     5641 ctcaccgccg cgaatggtga tcgagggcgc cgggtccagc tgccaggaca ccagcggcac
     5701 cggctgcaga aaactgtcgc cgttgccggg ccgcgcgccg gccctggcga actcggtcgc
     5761 gaggtactcg agcgtcagcg cctcgccttt cgagcccggc cagcggcctt ccatctcgtc
     5821 ggcggacagc gccgcgatgt gacggtgcag ggccgcctcg ttgatcggcg cggtcggctc
     5881 actcgcggcc acaccggccg ggacggcttg tagcaatacg ccggcacagg ccagcgcaag
     5941 agggatgact tgtcgcttca ctgcttcact ccattcgttg tgggccgcgc ctgccgatgc
     6001 tcagcggccg cgcagctccg cgatccgcat cgccagctcc agcgcctgct cgtagttgag
     6061 gcgcgggtcg acctgcgaaa ggtatgcccg gttcagatcg gcctcggtca gcccgcgcgc
     6121 cccgccgatg cactcggtga cgtcgtcgcc ggtcagctcg aagtgtacgc caccgaggta
     6181 gctgcccatc tcctcgtgga tgcggaaggc ctgctcgacc tcggacagga tgcggtcgaa
     6241 gctgcgcgtc ttgatgccgc tcgaggtgct ctcggtgttg ccgtgcatcg ggtcgcacac
     6301 ccacagcacc gtggccccgg cggcgcgcac ggcctcgatc atcggcggca agtgctcggc
     6361 gatcctggcg gcgccgaagc gatggatgag tgccaggcgg ccgggctcat tgtcgggatt
     6421 gagcgtctcg atgagcgact gcagccactc gatgctcatg ccggtgccga ccttcaccgc
     6481 gatcgggttg gcgatgccgc ggaagtactc cacatgcgcg ccgtccacct gcgccgtgcg
     6541 catgccgatc caggggaaat gggtggacag gttgtaccag cgattgttgc gcgcgatgaa
     6601 acgcgtctgg gcctgctcgt acagcaggtg caggccctcg tgcgaagtga agaaatcgat
     6661 gcgccgggtg cggtgcaccg gctggccgga aatgaactcg aaaaagtcca tcgagtcggt
     6721 gatgctcttg acgatccgct tgtaggcctg ctcgaagggc gtcgactgcg cgaagccgat
     6781 gttccagtac tccggatgat gcaggtcggc gaagccgccc tcggccagcg cgcggacgaa
     6841 attcagcgtc agcgcggcgc ggttgtagcc ggtcagcatc agctccggat cgggcgtccg
     6901 gtcggccagc gtgaaggcgt tgcgattgac caggtcgccg cggtagctgg gcagcgaggt
     6961 gccctcgcgg gtctcatggt cggcggagcg cggcttggca tactgcccgg ccatgcggcc
     7021 gacgcggatc acgggtttct tgaggctgtg cagcagcacc acgctgacct gcagcaggat
     7081 cttgagcttg cgggcgatct ggtcggagtt gcagtcgtcg aaactctcgg cgcagtcccc
     7141 gccctgcaac aggaaggcct cgccgcgctg ggcgcgggcg atctgggcct gcagcgcctc
     7201 gacctcccag gacactacca gcggcggcaa cgcgctgagg cgcgcgatca ccgacgccac
     7261 cgcgtccgtg tcgggataca tgggctgctg gccggccacc ttgctctgcc agctggccgg
     7321 gtgccactcg cgcggctcgg gattggttgc catgaggtca tcctaccgcg cattgccgca
     7381 ctgcaaaaga cccgccggcg gccccgtgac tggcgtgttt cgcgccgagt cgcgataatt
     7441 gccggaccat cgcgagcccg ctccccggag tccgggctcg cggtccatgt ggatccggcc
     7501 accgcgcggt cccagtctac ggagcaagcc agatgcatcg aattctcgtc ctgggggccg
     7561 gcaagatcgg ggcccttatt tccggcctgt tggccgagtc cggtgattac cgggtggacc
     7621 tggccgacgt cgacgccgcc gcggcgcagg ccgtggtcaa ggcgcacggc ctcgatgctg
     7681 tgcgggcctt cggcctggac gccaccgacc gggcggcact ggacgcccac ctggacgccc
     7741 atcccgccga cgccgtgatc tccggcctgc cctatttctg caatgtgccg gtggccgagg
     7801 cctgccgggc ccgcggcatg cattacttcg acctgaccga ggacgtcgcg gtcaccgagc
     7861 aggtgcgcaa gatcgcggcg ggcgccgaca ccgccttcgc gccccagtgc gggctggcgc
     7921 cgggcttcat cagcatcgcc gccaacgagc tcgccaagca ctttgccgag ctgcgcaccg
     7981 tgaagctgcg cgtcggcgcc ctgccccagc accccaacaa cgtactgaaa tactcgctga
     8041 cctggtccac cgacggcttg atcaacgagt acggcaacat gtgcgaggcg gtcgagaacg
     8101 gcgagaccat caaggtgctg ccgctcgagg gcctggaaga aatcgagatc gacggcacca
     8161 agtacgaggc cttcaacacc tccggcggac tcggatcgct ggccgattcc tggcagggtc
     8221 gggtgcggcg catgaactac aagaccatgc gctaccccgg ccactgcagc cagatgcggc
     8281 tgctgatgaa cgggctcaag ctcaacaacg accgcgacac gctcaagcgt atcctcgaga
     8341 acgcggtgcc gcgcaccctg caggacgtgg tcatcgtcta cgtctcggtg gtcggcaagc
     8401 aggacggcga gctgcgcgag gaaaactacg tcaacaagat ctacccgcag gtcatcgccg
     8461 ggcggctgtg gtccgcgatc caggtcacca cggcggccgg cgtatgcgcc gtggccgacc
     8521 tggtgctgaa caaccccggg gcacaccggg gcttcgtgac gcaggagagt ttccagctca
     8581 gggaggtgct cgacaaccgc ttcgggcgct actacgcaca cggcggcacc tacgaggtgt
     8641 cggcccgcat gatcgccacc ggcgaggtcg ggcaccaacg cgcaaaggga gcatgagatg
     8701 caggatattt tgcagcgcct cggactggct gaggagaact ccggcgcctg ggccggtacc
     8761 cgcgaactgc cgggcagcgg ccccgtcatc gagtccttca accccgccac cggccagcgc
     8821 atcgccagcg tgcgcagtgc cagcgaggac gagtacgagg agatcatcgg cagcgcccgc
     8881 gaggccttcc acctgtggcg cgccatgccg gcaccgcatc gcggcgaggc cgtgcgcttg
     8941 tgcgccgagg cgctgcgccg gcacaaggac gcgctcggca gcctggtgag cctcgagatg
     9001 ggcaagatca aggccgaggg cgacggcgag gtgcaggaaa tgatcgacat cgccgacttc
     9061 gcggtcggcc agtcgcgcat gctctacggc cgcaccatgc actcggagcg gcccggccac
     9121 cgcatgtacg agcagtggca tccgctcggc gtggtcggca tcatcagcgc cttcaacttc
     9181 ccggtcgccg tgtggagctg gaatgccctg atcgctgccg tgtgcggcaa cgtctgcgtc
     9241 tggaagccct cgcccaagac gccgctgtgc gccatcgccg tgcagaaaat ctgcaacgag
     9301 gcgctggccg acggcggctt cccgccgatc ttcaacgtgt tcgtcgaggc cgacgtcgcg
     9361 atggcggaag ccttcgtcga cgacgagcgc gtcgacctgg tctcgttcac cggctcgacg
     9421 ccggtcggcc gccgggtggc gcagcgggtc gcggcgcggc tcggccgctc gctgctggag
     9481 ctcggcggca acaacgccct gatcgtcgac gagaaggcca acctggaact cgccgtgccg
     9541 gcgatcgtgt tcggcgcggt cggcaccgcc ggccaacgct gcaccacgac ccggcgtgtg
     9601 ctggtgcacg agaagctgtt cgacgagctc gcccaccggc tgatgcatgc ctattcgcag
     9661 gtcaaggtcg gcgacccgct cgacccgaag accctgatgg ggccgctgat cgacgcagac
     9721 gctgtactgc gctacgaggc ggcgatcgag aagctgatcg cccgcggcgg aagggtgctc
     9781 accggcggtc acgtcatcga ccggcccggc cacttcgtgc agccgaccat cgtcaaggcc
     9841 agcaacgagt gggacgtcgt gcaggaggag accttcgcgc cgattctgta cctgatcccc
     9901 ttccatcact tcgacgaggc cgtcgcgctg aacaacgcgg tgcgccaggg cctgtcgtcc
     9961 gcgatcttca ccgacgacct gcaggccgcc gagcgcttcc tgtccgccac cggcagcgac
    10021 tgcggcatcg ccaacgtcaa catcggcacc tcgggcgcgg agatcggcgg cgcgttcggc
    10081 ggcgagaagg acaccggcgg cggccgcgag gccggctcgg attcctggca ggcctacatg
    10141 cggcggcaga ccaacaccat caactacagc cacgagctgc cgctggcgca gggcatcaag
    10201 ttcgacctgg agggttgagc ggccggcgcc ccttgccggc gcgcgctgtc agccggccgg
    10261 ctcgatcggc tcgaagcggt tcaggccgcg gttcttgcgc acctggtcgc aggcgaagac
    10321 atggccgctc atggcgatcc agacgcccgg cgggcaggtc tgcaccgcgg ccaccgccat
    10381 gccgatgttg aacacggcgt cggaggcctg gaaacgcgcc gggctcaacg cgccggtcag
    10441 cacgatggtc ttgccctcga taccgctcaa tgcacgcgca gtttcgacca tggtgtcggt
    10501 gccgtgggtg acgatgatgc gccgctcctc gcgcgcggcc gcggccgcgt gaatggcagc
    10561 gcggtcagcg tcgaccattt cgaggctgtc cttgcgcatc aacgggatga cctcgaactc
    10621 gaaggtcacg ccgccctcgc gcaggatctt gccgaccacg gtctcgccga cctcgaagcg
    10681 gctcatggcg tcgaagtaga ccttgtcgat cgtgccgccg gtggtcagga aaacgatgtc
    10741 catggccgtg ctcgcgaaaa ccgattgccg ggctgcggcg aaatgccgtg cacagccgga
    10801 aaacccgact ggcgtgccca ccaccgtgaa gataccatga gcctatgaaa accctgcggg
    10861 ccctgttcct gtccctcgcc tgcctcgggc tactcgcggc gtttggccag gccgcttcgc
    10921 aaggcggcgg ccaggacgcc ggcgggcgcg ccctgctgct ggagctcgac ggcgccatcg
    10981 gcccggccac cagcgactac atcgtgcggg gaatccgcaa ggcggccgag gagcaggcgc
    11041 agctggtaat catccagatg gatacgcccg gcggcctcga caccgcgatg cgggacatca
    11101 tcaaggccat cctgagttcg cccgtgccgg tcgtgacctg ggtcgcaccg agcggctcgc
    11161 gcgccgccag cgccggcacc tacattctct atgccagcca tgttgcagcc atggcgccgg
    11221 cgaccaatct cggcgccgcc acaccggtac cggtgggcgg cggccagccc gcacccccgg
    11281 cggaggaacc cgcggggcgt gaccgggcgg aagaccgcga tgccgacaag gcggacgagc
    11341 aagccgccga ggcggacgat cgcagggaac cggcgccgat gcgcggcggg cccgaggcca
    11401 aggcgatcaa cgacgcggtg gcctacatcc gcggtctcgc cgagcggcgc ggccgcaacg
    11461 ccgactgggc cgagcgggcg gtgcgggaat ccgccagcct caccgccgag cgcgccgtcg
    11521 aacagaacgt catcgacctg gtcgccgccg acatgggcga actgctgcgc aagatcgacg
    11581 gccgcaaggt gtccaccgag tccggcgacg tggtgatcca gggcgagggc ctggccgtcg
    11641 agcgggtcga gcccgactgg cgcaccgaat tgctggccgt catcaccagc cccacggtcg
    11701 cctacatgct gctgctggcg ggcatctacg ggctgatctt cgagggctac aatcccggcg
    11761 cgatcctgcc cggcgtggta ggcgcgatct gcattctgct cgcgctgtac gccttccagg
    11821 tgctgccggt gaattacgcg ggcctggcgc tgatcgtgct gggtatcttg ctgatgatcg
    11881 gcgaggtctt catgcccagc ttcggcgcac tcgggctggg cggcatcgtc gctttcgtgg
    11941 tcggctccat catcctcatg gacaccgacg tgccgggctt cggcattcca attgtaatga
    12001 tcggctcggt ggcgctgctc ggcggcagcg cggtcatggc catcatctgg ttcgcggtgc
    12061 gggcgcgcaa ccggccgatc gtcagcggcc gcgaggacat gatcggcgcc gcggcgatcg
    12121 cggtcgacga ctttgccggc cgcgggcacg tgcgcgtcca cggcgagcgg tggaacgccg
    12181 agagcgccgt gccggtgcac agcggccagc cgctgcgcgt cacggacatg catgggctcg
    12241 tgctcaaggt cgagccggac gaatccaagg agaactgaca tgctaggtta tttcggcaca
    12301 ttgctgctcg gcggcgtcct gctggtgctg ctcagcgcca tcaagatcct caaggagtac
    12361 gagcgcggcg tcatcttcca gctcggtcgc ttctacaagg tcaagggacc cggcctgatc
    12421 atcgtgatcc cggtcatcca gcagatggtc aaggtggacc tgcgcatcgt cgtgatggac
    12481 gtgccctcgc aggacgtcat ctcgcaggac aacgtgtcgg tcaaggtcaa cgcggtcgtg
    12541 tacttccgca tcgtgaatcc ggagcgcgcc gtgatccagg tcgagaacgt ctacgaggcc
    12601 accagccagc tggcccagac caccttgcgc tccgtgctcg gcaagcacga gctcgacgaa
    12661 atgctctccg agcgcgacaa gctcaacacc gacatccaga acatcctcga cgagcatacc
    12721 gacaactggg gcatcaaagt caccaacgtc gagatcaagc acatcgacct gaacgagagc
    12781 atggtgcgcg ccatcgcgca gcaggccgag gccgagcgcg ccaggcgctc caaggtcatc
    12841 cacgccgagg gcgaaatgca ggcctctgca cggctggtcg aggccgccca gcagctcgcc
    12901 acgcagcccc aggcgatgca gctgcgctac ctgcagaccc tgagccagat cgccaacgag
    12961 aagaccaaca ccatcgtctt cccgctgccg ctcgacctga tcgggccgct gatggagcgc
    13021 ctgggccagc cgggagcgac ggcaacgcgc gacgaccagt cccgctgagg cggcgtcagc
    13081 cggccgggct ggcggggatc caggccaccg acagggcgcc cggcccaagg cgcgcgccca
    13141 tggtcgcgct catcacggcg aagtgcaggt cgagccgcgc ggtggcggcg cgcgactcga
    13201 gggcctggta ggccggcatc tggcggatga cacgcggatc gccgccgaag ctcagggtga
    13261 cggcgccgcc gcccagcccc tgcccgatcg cccgcgtggc gcgctcgagc gccacggcgc
    13321 atgccgcgcg aaaaccccgc gcccgggcga cgatccggcg gcgcccgccc tgcacttcgg
    13381 tgaccggctt gacgtccgaa atgatggcca gcgcatgatc cacccggccg atgccccggc
    13441 cgcgaccgtc gaggccgcgt cggcgcaggt accaggtgtc gcccggcacc agcagacagg
    13501 tagttcgcgc cacctcttcg cgcagtgtgc gccgcacggc ctcgaagccg tcccggcctt
    13561 cctcgagcag ttgcagcgcc cggcacagca gcgccgcttc accggcgcag atcgaaccgc
    13621 tgtcgagcac gcgcacggag aatccgccac cctggccctc ctcttgccgc cgttcccggt
    13681 agcgctgcag gatcgcgtag gaggcttcgg tggcgcgcgc gaacatgtcg ctgaagtcgg
    13741 cgccggccgt cagcaccagc acgcggtcga aatcgagcac caggtcgcgc aggaagatgt
    13801 cgcagatctc gctcgccccg caggcgctcg aacgcgctgc cacggcgcga tcgccgatgt
    13861 aacgccggta cagcatgagc gtctgctccg ggtcgcgctc gtccagccag ctccgtccgt
    13921 ccagctccag cttcgaaggc aggatggcga tgccgttggc ctccagcacc gagtccggaa
    13981 gatcgcagga agcatcaacg acgaggccga ttcgcatcag gggcgccggt ggcggaactt
    14041 gcggggattg tggttctccc ggcgggaggg cgtcaattcg ccgggcgcag cccggcctgc
    14101 agctcccgca ccttctgcca ctgcgcctcc agccgcgcat gggagacttt ttcgttgacg
    14161 ccgagctgct gcgcgaacag ctgcacgcgg tactcctcca ccatccagcg gaacgcctcg
    14221 aaggcttcgg ccgccgccgg cgtcgaccag tccgtggctt cgccctcgcg cagccgccgg
    14281 cgctgcacgg cgaggtccag cacctgccgc gcttcgccct tgccgagcgc gagccgctcc
    14341 atgcgcagca tgacggcctg caggtagcgc ggcacctgcc ggagtcgctc gggcggcgcc
    14401 gccaacagga acccgggccg gaccagcgcc ccgagctgcg cggcgatgtc tgcagccgca
    14461 tcgctcgcac ccggcccggt caggccgtcc gccaggcgcg ccaggcaggc atcgcgattc
    14521 gccaggatgt cgcgcaccag cgccagcatg cgctcgccct ccggcatgat gtcggcccgg
    14581 cccccgtcca gccgctcgcg aaatgcatcc acggtgcgcg ggacgtcctt gccgcccggc
    14641 aggaacaccg ccgcgaacgc cgcgctgacg atgtcttccg cgagatcccg gccctcggcc
    14701 aggtcccgac cgcgcagcac cagcgtgcga tccgtccgcg cctgttgcag cagcgcacgg
    14761 tgctgttgcg ccagggccag catgaacaac cgcagcacgc cgcgacgatg cacccgggcc
    14821 gccgcggcgc cgctcgagaa cagccggcag gacaccgtgt tgccgtcgtc ctggagcgcc
    14881 gggaacaggg cctggcgcaa tgcgccgtgc gtgacttcga ccgtggccgg catggcgcac
    14941 tccggccagg ccttcagtcc ctgcccgctc cagccggctg cggccggggc cgacaccggg
    15001 cccgcgcccg gcgaatgcgc ccgctcgagc gcggccaggt cgcgcgatgc cgccagcggc
    15061 gcgccgtcgt cgccgcgcac ctcgatacgc attcgcagcc aaggttcgag ttccagtcgc
    15121 tgccaggttt cgggctcgac ctgcaggccc gcggtgcgct gcagggccgc ggcgagcgcc
    15181 gcgcgcaggc gaccctgtcc cggccggagc aaggccaggc aggcgcgcgc gtactccggg
    15241 atcggcacga tgcgccggcg cgccgacttg ggcagcgcgc gcagcatcgc gacgactttc
    15301 tcctcgagcc agcccggcac gagccattcg acgcgctcct ccttcaagag cggcagcagg
    15361 tggcggggca cttcgaccgt gacgccatcg tcctccgccg ccggctcgaa gcgatagcgc
    15421 agcggcaggc ggttgccgcc gacctcgagc gtgtccggat acgcctccgg gtccggcagc
    15481 gcagcaccgg tcagcaggtc gtcgagggcc atgaacagca actcgggctg cgccttctcc
    15541 gccttgcgcc gccagcgctc gaaggccggc gtcgaacaga tcccgggcgg cacgtgggcc
    15601 gcatagaacg ccgcgatcgc cgcatcgcca gccagcagct cgcggcgccg cagcttggcc
    15661 tccagcagct gcacctcgcg cagcagccgc cggttggcgg ccaggaaccg gccgcgggtg
    15721 cgcagcgtcc cggctgccag ggcgtcgcgc aggaagatct cacgcgcgcc cgccgcatcc
    15781 acgcgggcga aatccacgcg gcgattcgcc gccagcacca gcccgaacag gctcacctgt
    15841 tcgcgcgccg cgacgaagcc gcgttccgcc tgccagtgcg ggtcggacca gctgcgccgc
    15901 accaggtgcg cccccgccga ctcgatccag ccaggctcga ccgcggcggc catccggccc
    15961 cacacccggc cggtctcgac cagcgcggcg accgtcaccc agcgcggcgg acgcgccgcc
    16021 agcgcagagt cggggaacag ccggaaacgc aggttgcgcg gcccctggta ctcgcccttt
    16081 tcctgcagct ggccgatatg cgacaacatg ccgctgagaa tggcgcgatg cacggcggcg
    16141 tagtcggcgg gcagctcgtt ctgccgcagg cgcagctggt ggacgccctc gaccagctgg
    16201 cggtgcacgt cctgccattc gcgcagccgc aggaacgaca ggaagtggtc gcgacaccag
    16261 cgccgctgcg cactgccgga gtcgtgccgg cgccgttcct gccaggcctc ccacagcttc
    16321 accagcgaca ggaagtccga gcgtgggtcc tggaacaagg cgtgcttttc gtcggcgctg
    16381 cccgcggcat ccgccggccg ctcgcgcgga tcgcggattg acaggccggc cgcgatcacc
    16441 aggatctcct tcaggcagcc ggccggaccc gcggccagca gcatgcgccc cagccgcggg
    16501 tcgaccggca ggcgcgccag tcggcggccg aggtcggtga cgacccgctg ttcgtccacc
    16561 gcgccgagct cgcgcagcag ccggtagccg tcgttgatga aacgccggtc cggcgggtca
    16621 aggaaaggga actcggcaat gtcgccgagg cccagcgtct ccatctgcag gatgacgctc
    16681 gccaggttgg tgcgccggat ctcggggtcg gtgtactcgg gccgcgcgtc gtggtccgcc
    16741 tcgtcgtaga ggcggatgca gacccccggt gcctcgcggc cgcagcggcc gcggcgctgc
    16801 caggcgctgg cctgcgagat cggctcgacc tgcaggcgct ggattttcga gcgatagctg
    16861 tagcggctga tgcgggccag gccggagtcg accacgtagc ggatgccggg cacggtcaag
    16921 gacgtctccg cgacattggt ggcgagcacg atgcgccgcc gccccgcgcg ccggaacaca
    16981 cggtcctggt ccgtcgcgga aaggcggcca tacagcggca gcacttcgac gccgggcgga
    17041 tgatgcttgc gcagcgcctc ggctgtgtcg cggatttccc gctcgcccgg caggaagacg
    17101 aggatgtcgc cctcgc
//
LOCUS       DAOMPG010000022        16965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1487_length_16965_cov_4.985452, whole genome shotgun sequence.
ACCESSION   DAOMPG010000022 DAOMPG010000000
VERSION     DAOMPG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16965
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_16965_cov_4.985452"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="rph"
                     /locus_tag="PLS34_02130"
     CDS             <1..374
                     /gene="rph"
                     /locus_tag="PLS34_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRP86304.1"
                     /translation="CDVLQADGGTRTAAITGAYVALVDALGQLRRRGALQTDPVHGQV
                     AAVSVGLYRGLAVLDLDYAEDVEAETDMNVVMNEAGAFVEVQGTAEGHAFRREELDRL
                     LDLAAGGIGELLALQRQALQE"
     gene            371..997
                     /gene="rdgB"
                     /locus_tag="PLS34_02135"
     CDS             371..997
                     /gene="rdgB"
                     /locus_tag="PLS34_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRP86305.1"
                     /translation="MIAAGGRVVLASGNAGKAREITALLAPAGIEVVSQAAFGIEPAE
                     ETAATFIENALIKARHAAAGSGLPAIADDSGLVVDALSGAPGVHSARYSGSGDDAANN
                     RKLLAALGALPPERRGARFICVAVMLRHPGDPLPLVCEGRWEGSIALAARGDGGFGYD
                     PLFLPRGDTRTAAQLSPQEKNRLSHRGQAFGALLAGLPAAVSAAGGAP"
     gene            1042..2163
                     /gene="hemW"
                     /locus_tag="PLS34_02140"
     CDS             1042..2163
                     /gene="hemW"
                     /locus_tag="PLS34_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRP86306.1"
                     /translation="MHLPWCVRKCPYCDFNSHELRGAAPFAAYVDALLEDLDEDLPLA
                     AGRKVATVFFGGGTPSLCPPSLIGRFLEGLAARLPLASDTEVTLEANPGAVERGRFGE
                     LAAAGVNRVSLGVQSFQPHLLTAIGRIHDAREAHAAIDELARAGIGNFNLDLMFGLPG
                     QTVEEAMADIDAALAAAPPHLSLYQLTLEPGTPFHRHPPSLPDDDACWRMEEAAAERL
                     EAADYRRYEVSAWARPGMECRHNLNYWVFGDYLGVGAGAHGKLTLADGRIIRRQRRRR
                     PLGWLEGARVEEQRELSAEERLFEYMLNALRLADGCRLDDFERLTGLPAAAALPGLEQ
                     GRRLGLLASPDGAGWRSTPLGRRFLNDAQALFLPSSSPA"
     gene            2621..2830
                     /gene="rpmE"
                     /locus_tag="PLS34_02145"
     CDS             2621..2830
                     /gene="rpmE"
                     /locus_tag="PLS34_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRP86307.1"
                     /translation="MKPNTHPEYTEINVVCSCGNTFTTRSTLGKELHVEVCSQCHPFY
                     TGKQKIVDTGGRVDKFRRKYGGMSA"
     gene            3039..4340
                     /locus_tag="PLS34_02150"
     CDS             3039..4340
                     /locus_tag="PLS34_02150"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRP86308.1"
                     /translation="MSDKTLTLVNEADGSRLEMPVRSGTAGPDVVDIGALYKKFGVFT
                     LDPGYVSTASCESRITYIDGDNGVLLYRGYPVDQLAERSSFLEVSYLLLYGELPTRSQ
                     LEQFTNIITTHTMINQTLLRHFNGFHHNAHPMAMLASVVGSMSAFYHDSTDIENPRHR
                     EIFAHRIIAKIPTIAAAAYKHSVGQPFIYPRNELDYSSNLLNMFFAVPTADYKIDPVA
                     AEALDLLFILHADHEQNASTSTVRLAGSSGTNPYAAIAAGIATLWGPAHGGANEAVLQ
                     MLEKIGSVDNIPKFIAKAKDRDDPFRLMGFGHRVYKNFDPRAKLIRTMCHKVLNRLGH
                     TDSPLFELALRLEEIALEDEYFIERKLYPNVDFYSGIIYRAIGIPTSMFTVMFAIART
                     VGWVAHWQEMITDPQRRIGRPRQLYTGAGTRDYTPIERRGA"
     gene            complement(4374..5030)
                     /locus_tag="PLS34_02155"
     CDS             complement(4374..5030)
                     /locus_tag="PLS34_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86309.1"
                     /translation="MPKRAIQRAGELRIRVAEEAARIMREQGVRDFLLAKRKAAERLG
                     IVDRHALPANQEIADALAAQQRLFGGAAHHDNLRVLREAALDAMRLLQGFNPRLVGPV
                     LAGTATLHTDVSLHLFAATPEEVAFLLIERGGRYRSGERRVRLAGGEYASFPSFEFEV
                     HGVPVEVTVFPEHGLREAPVCPVNGGQMRRARREEVEALLAAASPAADDAAFRPSGRD
                     "
     gene            complement(5046..7472)
                     /locus_tag="PLS34_02160"
     CDS             complement(5046..7472)
                     /locus_tag="PLS34_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRP86310.1"
                     /translation="MRVLLRLFYGIVALVAMSLTALALAAIGAYYYVAPSLPGVESLR
                     DVRLQVPMRVYSRDGLLMAEFGEQRRIPLRIDEVPEQLQRAFLAAEDDRFFEHPGVDW
                     QGLTRATLAVIATGEPAQGGGTITMQVARNFFLSREKTVSRKVREIFLALRIEHLLSK
                     EEILDLYLNKIFLGQRAYGVGAAAEVYFGKQVGELSLAEMALIAGLPRAPSLDNPVTS
                     PQRARQRRAYVLRRLFETGAIDREAMEAASAEPVLSRIHGAQVRLRAPWVAEIARQEV
                     LDRYGPEAYTAGYSVVTTVDSRLQPSAQRAVRQGLESYDLRHGYRGPVAEVEALPEGA
                     EDVEALLDSYPEPAGLRSALVLAVDDTGADVFVRGAGEARLDFEGMRWARRYIDENNR
                     GPAPERPADVVATGHVLLLDRRDDAWALSQVPAVQGALVALDPRDGAVVALSGGLDFE
                     QSKFNRATQARRQPGSAFKPFVYSAALERGLTAASIMIDAPVVYDDPVLEDTWRPQNY
                     TGRFYGPMRLREALVQSRNLVSIRVLEQIGVPFTVNYVSRFGFPADSLPRNLTLALGS
                     GGVTPVELTAGYAAFANGGFRIAPYFIDRIYGPDDELLHQAEPPWACAECEYGRLPEF
                     AREPAAANDESGEATPADAASTQPPQAIDAGNAWIISSMLEDVVRRGTGARARELGRN
                     DLAGKTGTTNDGRDTWFAGYNGDLVATAWVGFDQERPLGRGETGATTALPVWMAFMRG
                     ALEGMRSSRPAEPPGLVTVRVSADTGRLARAGEPDAIFEVFRVGEVPPPPEGGEERYP
                     DGSSEEDPLF"
     gene            7792..8853
                     /locus_tag="PLS34_02165"
     CDS             7792..8853
                     /locus_tag="PLS34_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRP86311.1"
                     /translation="MLFGRSNKPLLGLDITTSSVKLIELSKSGDTYRVEAYAAEATPL
                     NAVNEKAIVDAQVVGEAVRRALKRSGARSREAAVAISGDAAITKIIQMPANFTGAELE
                     GQVELQADQYIPFPMDEVSYDFEVIGSTEKDPDMIDVLLVATRTENVEQRQAAVEAAG
                     LKTRIMDVEPFAVENACLLLRHQMLDEGMGHNVAVMDFGASSTTFSVLRDLKVIYTRD
                     FAFGGQQLTEEIMRTYGLTLEEAGRAKKEGGLPSNYEPEILEPFIDDMTQQVSRSLQF
                     FLASGTNREQPEQILICGGCANIPGVADVIGSRVGIPTQIGNPLGMMKVSSKARSQGV
                     LKDATALLTACGLALRSFD"
     gene            8853..9455
                     /locus_tag="PLS34_02170"
     CDS             8853..9455
                     /locus_tag="PLS34_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRP86312.1"
                     /translation="MHRINLLPWRAELRKERQKNFAVATVGAIVLGLLIMWAVNTILQ
                     GRIDYQNSRNQRLQQEIAVLDEQIKEIRNLRLRKDRLLLRMAAIDELQRARPEVAHLF
                     DEIVKVMPDGVYLTEFTQNGRSLLFKGVAESSTRVSALMRNIASSRSMADPRLQVVQS
                     GQQQRGRSAPAPRGPGRQAEFALAARQAGGADEGDEEVLQ"
     gene            9452..10081
                     /locus_tag="PLS34_02175"
     CDS             9452..10081
                     /locus_tag="PLS34_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRP86313.1"
                     /translation="MKFFDQLRSLDTQDVGRWPPLFRGLAIGIIFVLVAVGATYYFVW
                     RVKIPQLEQVQRQEQDLRTTFESRQRLAANLEAYRDQLEEVERTFGAMLRQLPGETEV
                     PSLLVDISQTGLAAGLEERLFQPMSEVSREAYSELPIKMQVVGTYHELGNFVSGVVAL
                     PRIVTLHDVVLKPEQAERGRATDAPPSNRLVMDVTAKTYRYLEDEGAGQ"
     gene            10078..10599
                     /locus_tag="PLS34_02180"
     CDS             10078..10599
                     /locus_tag="PLS34_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRP86314.1"
                     /translation="MNAPSRILLLTATLALALVASGCSRGMSDLEGYIAEVKARPGGR
                     IEPLPEIKTYETFRYNPGDARSPFVPDVPTAGSVAGGVRPDADRRREYLESFPLDTLN
                     MVGTVSMGGRLYGLVQTSDKLVHRVLAGNYIGQNDGRIVSITSSEIQLLEIIPDGLGG
                     YIERAASISLGDL"
     gene            10620..12836
                     /gene="pilQ"
                     /locus_tag="PLS34_02185"
     CDS             10620..12836
                     /gene="pilQ"
                     /locus_tag="PLS34_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRP86315.1"
                     /translation="MMRDQWRSNRTVGTGTRSRLGWWTVAVLPLLWWSGAASAQQDLQ
                     LTGLDVSTLPGNRVVLVLTTSGPAPEPLAFTIEDPARISLDLPGTKLGVAERRRDVKR
                     GVLDTVMLAEAQGRTRVVLNLDLMVPYETAVDGDRITVTLGGPAAPSQAARASAFGQP
                     SGSVAVAAPAAGAAITGVDFRRGADGAGRVLVNLSDPSVAIDIREDLDRTVVEFRNAS
                     LPDNLLRRLDVLDFATPVQTIDAVRFPGGARLVINAAGNYEQLAYQADNQFSIEFRPV
                     AEQDPSEYGLFDERKEYTGEALTLNFQDIEVRAVLQLLAEISGLNVVVSDTVRGNVTL
                     RLDSVPWDQALDIILTTKGLDMRQVGTVVLVAPAAEIAAREKQELEAKKDIQELAPLR
                     LEFLQVNYAKAADLAVLLTETAGRGVGSGTGTRTMLSERGNVSIDERTNTLLINDTAE
                     RIADIRRLVAALDIPVRQVLIESRIVVANDDFSRELGVRMGGTVVRENGSDGLITITG
                     SGQGTDTIVGSGLNNLNQTGSAFPVALPSLGDRYNVNLPANEPAGRIGLAILGSDYLV
                     DLELSAAQAEGRGEIVSSPRVITANQKQALIEQGVEIPYQEAASSGATATQFKKAVLS
                     LTVTPQITPDDRIIMDLEVTQDTVGQFVPSATGGQVPSIDTRRIITQVLVNDGETVVL
                     GGIYETERRDAEDKVPLLGDIPGLGRLFKRNSSVSNKRELLIFVTPKILREGSNIY"
     gene            12869..14797
                     /locus_tag="PLS34_02190"
     CDS             12869..14797
                     /locus_tag="PLS34_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP86316.1"
                     /translation="MRSSKFTRRVLGLPGLLAALFLVGCGSESTIVGPPGGGTPDSPV
                     ASITLIASSNVLPSGGAAVVTFSVLVRDANNNAVTGVPVTYSADSGLLQVIPDPDGVA
                     LPAAELSTGGDPTNRPITVTATTGSLSSSVTVNVTGTVLTLEGPDNVIQGDEVPFTAR
                     LQNSAGQGIAGRTVTIESAAGNTLSADSLTTNSQGQVSFTVTGTQGGADTISVSALGL
                     TATAPLSVSADDSVVFLEPAAGTEIPLNVAQPVTVEWRVNNVPQAGQTINFSTTRGSL
                     SALSAVLDAAGQATVNLSATTAGPATLTARVGAAGPQTQRSIEFVALTPASIVVQASR
                     FTVGPGEQSTIVATVRDAANNPVKNKVVQFQIEDVTGGSLSVGTAITGSAGTAQTVYT
                     GGETISGSGDVTITAIVQENPALTATLGLTVARRQVDISIGTGNELFEPNTAQYEREF
                     LVQVTDSQGVGVADAAVQLSVRSKLYRKGFYVEGANDRWVVGPGAILCPDEDLNNNGQ
                     LNANEDFNGSGSIEAGNVVSVTPGDVVTDESGTAIFRIRYAQEFGNWVIVTITARAAV
                     QGTEFEDRSDHALEITAADANVNQSPPGGLISRFGGGDLDPNPDPTDEGLTVPEYATR
                     LAELGFAGGRPLGCRTED"
     gene            14870..15397
                     /gene="aroK"
                     /locus_tag="PLS34_02195"
     CDS             14870..15397
                     /gene="aroK"
                     /locus_tag="PLS34_02195"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HRP86317.1"
                     /translation="MALPNIFLVGPMGAGKSAVGKQLARMLRRQFHDSDAEIEARTGV
                     DIPFIFEKEGEVGFRRREREVIEALTLLDGIVLATGGGAVLDPANRHALASRGLVVYL
                     EASVDQQLERTRLSSHRPLLDTVDPAERLARLLQERAPLYREVAVLAIPTDGRLVREV
                     AQEIKRTLAALSEED"
     gene            15406..16485
                     /gene="aroB"
                     /locus_tag="PLS34_02200"
     CDS             15406..16485
                     /gene="aroB"
                     /locus_tag="PLS34_02200"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRP86318.1"
                     /translation="MHALNVPLGDRTYPVLIGRGLLGRAEVLAPYLDGRQVLVVTNEI
                     VAPLWLKPLRAALADYELAVHVLPDGEEQKSLETLGLLFDVLAEHRFNRDATVLALGG
                     GVIGDVAGFTAACWQRVTAFVQLPTTLLAQVDAAVGGKTAVNIPAGKNLVGAFHQPSA
                     VIADVATLGTLGRKEYCAGLGEVVKYGLGLDSGLFEWLERDLDAVIDREPEALERMVY
                     WCCALKAAVVAEDEREQGRRALLNLGHTFAHAIEAATAFGAWLHGEAVAVGLVMASEL
                     AVRMGMLDRDIAARVEALIAAAGLPTRPPPVGAERLKAFMQLDKKIAGGRLRFVLPDG
                     LGSCVVRADVPEAELDAVLAMAAPR"
     gene            16482..>16965
                     /gene="dgt"
                     /locus_tag="PLS34_02205"
     CDS             16482..>16965
                     /gene="dgt"
                     /locus_tag="PLS34_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRP86319.1"
                     /translation="MSEAPAPWAACETRSPGRRYPEPPPRYRGEYHRDRDRIIHSTAF
                     RRLVYKTQVFVNHEGDLYRTRLTHSLEVAQIARTVAGALGLNTYLTEAICLAHDLGHT
                     AFGHAGQEALNRCMAEYGGFEHNLQSLRVVDELEEKYAEFPGLNLMFETREGILKHCS
                     A"
BASE COUNT         2722 a         5875 c         5786 g         2582 t
ORIGIN      
        1 actgcgacgt gctgcaggcg gatggcggca cccgcacggc cgccatcacc ggcgcgtacg
       61 tggcgctggt cgacgccctc ggccagctgc gtcggcgcgg cgcactgcag accgacccgg
      121 tgcacggcca ggttgcggcg gtgtcggtcg ggctctaccg gggcctcgcc gtgctggacc
      181 tcgactatgc cgaggacgtc gaggccgaga ctgacatgaa cgtcgtgatg aacgaggccg
      241 gtgcgttcgt cgaggtgcag ggcaccgccg agggccatgc ctttcgccgc gaggagctcg
      301 accggctgct cgacctcgcc gccggcggca tcggcgagct gctcgcgctc cagcggcagg
      361 cgctgcagga atgatcgcgg ccggcggccg ggtggtgctg gcgagcggca acgccggcaa
      421 ggcccgcgag atcaccgcgc tgctggctcc ggcgggcatc gaggtggtct cccaggcggc
      481 cttcgggatt gaaccggccg aggaaaccgc cgcgaccttc atcgagaacg ctctcatcaa
      541 ggcgcgccat gccgccgccg gcagcggcct gccggccatc gccgatgatt cgggcctggt
      601 cgtcgacgcg ctgtccggcg caccgggcgt tcactcggcg cgttactcgg gcagcggcga
      661 cgatgccgcc aacaaccgca agctgctggc ggcgctgggc gcgctgccgc cggaacggcg
      721 cggcgcgcgc ttcatctgcg tcgccgtgat gctgcgccac cccggcgacc cgctgccgct
      781 ggtctgcgag ggccgctggg agggctcgat cgccctggcg gcgcgcggcg acggcggctt
      841 cggctacgat ccgctgttcc tgccgcgcgg cgacacgcgc accgcggcgc aattgtcgcc
      901 tcaggagaaa aatcgcctga gccaccgggg acaggcgttt ggcgcgctgc tggccgggct
      961 gcccgctgcg gtgtcggccg ccggcggtgc gccatagcgg ccggcccccg ggtcgccgtc
     1021 ccgccggcgc tgggagtcta cgtgcacctg ccctggtgcg tgcgcaagtg cccctattgt
     1081 gatttcaaca gccacgagct gcggggcgcg gcgccgttcg cagcctatgt cgacgccctg
     1141 ctcgaggatc tcgacgagga cctgccgctg gccgccgggc gcaaagtggc gacggtgttc
     1201 ttcggcggcg ggacgcccag cctctgcccg ccgtccttga tcggccgctt cctggagggg
     1261 ctggcggccc gcctgccgct ggcgagcgac acggaagtga cgctcgaggc caatccgggc
     1321 gccgtcgagc gcggccgttt cggtgagctc gccgctgccg gcgtcaaccg ggtttcgctg
     1381 ggcgtgcaga gtttccagcc gcacctgttg acggccatcg gccgtatcca cgatgcgcgc
     1441 gaagcccatg ccgcgatcga cgaactggcg cgggccggca tcggcaattt caacctggac
     1501 ctgatgttcg gcctgccggg gcagacggtc gaggaagcca tggccgacat cgacgcggcg
     1561 ctggcggcgg cgccgcccca cctctccctc taccagttga cgctcgagcc gggcacgccc
     1621 tttcacaggc acccgccgag cctgcccgac gacgatgcct gctggcggat ggaagaggct
     1681 gccgctgagc ggctggaggc cgcggactac cggcgctacg aggtctcggc atgggcgcgg
     1741 cccggcatgg agtgccggca caacctgaac tactgggtgt tcggcgacta cctgggcgtc
     1801 ggtgccgggg cacacggcaa gctcacgctg gccgacggcc ggatcatccg ccggcagcgc
     1861 cgccgccgcc cgctcggctg gctggagggc gcccgggtcg aggaacagcg cgagctttcg
     1921 gccgaggagc gcctgttcga gtacatgctg aacgcgctgc ggctcgcgga cggttgccgg
     1981 ctcgacgact tcgagcgcct cacgggcctg cccgcggccg cggccctgcc gggactcgag
     2041 cagggccgtc gcctcggcct gctcgcctcg cccgacggtg ccggctggcg gtcgaccccc
     2101 ctcggccggc gcttcctgaa cgatgcgcag gccctcttcc tgccctcttc gagccctgcc
     2161 tgagccggcc acttatgcac atgaggggcg gcgcccgcct gccccgcggc tgctgcgccg
     2221 cggtctgctc ggcagtggct gaaagccacg gcggtgattg cgcaaccaat tgttttcaaa
     2281 agactgaacc atcgctggtc gctttttcgc caatctgaca accggggctt gattgacggc
     2341 tgcaaccgac ttgatcaagc cgctgttcac agagttttcc acagcttttg tggataaccg
     2401 ggaaaggcgg ttccgggttc gtcactagca cgctcaactt aaagtgtttc ctgagcagat
     2461 tgttgcgccg cagcgccgag gcaccgaaca gcccgatcca gcggtgccat ccgtgggcgg
     2521 catccggggc ggaaccgggc cggtgttgcc cttgcccggg accttcccag tcgctatcat
     2581 ggcgtgcttt tccactggtt gccgagtccg gaaggccgac atgaagccga acacccatcc
     2641 cgagtacacc gaaatcaacg tggtctgcag ctgcggcaac acgttcacga cgcgttccac
     2701 gctgggcaag gaactgcacg tcgaggtctg ctcgcagtgc catcccttct acaccggcaa
     2761 gcagaagatc gtcgacacgg gcggccgcgt cgacaagttc cggcgcaagt acggcggcat
     2821 gagcgcgtaa ctgcgcacgc caccaggatc gaacgcatca aggggcgcct cggggcgccc
     2881 cttttcgttg ccgccggccg ggacacgacc tgtggccttg gctataatcc gggattccgt
     2941 ccgtccgccc atggatccgc agcgcggccg ccagcgcgcc gccgcagcgt cttgcggccg
     3001 cggggcatcg cttcaacgca ccaggaaggc cagcagcaat gagtgacaag accctgactc
     3061 tcgtcaacga agcagacggc agccggctgg agatgccggt gcgcagcggt acggcgggcc
     3121 cggacgtggt ggacatcggc gccctgtaca agaaattcgg cgtgttcacg ctcgatcccg
     3181 gctatgtgtc gaccgcgagc tgcgagagcc ggatcaccta tatcgacggc gacaacggcg
     3241 tgctgctgta ccgcggctac ccggtcgacc agctcgccga gcgcagcagc ttcctcgagg
     3301 tgtcgtacct gctgctctac ggcgaactgc cgacccgcag ccagctcgaa cagttcacca
     3361 acatcatcac gacgcacacg atgatcaacc agacgctgct gcgtcatttc aacggctttc
     3421 accacaacgc ccacccgatg gctatgctcg cctcggtggt cggctcgatg tcggccttct
     3481 atcacgactc caccgacatc gagaatccgc ggcatcgcga aatcttcgcc catcgcatca
     3541 tcgccaagat ccccaccatc gccgccgcgg cctacaagca ctcggtcggc cagccgttca
     3601 tctacccgcg caacgagctc gactattcga gcaacctgct gaacatgttc tttgcagtgc
     3661 cgaccgccga ctacaagatc gacccggtcg ccgcggaggc cctcgacctg ctgttcatcc
     3721 tgcacgcaga ccacgagcag aacgccagca cctcgaccgt ccgcctggcg ggcagctccg
     3781 gcaccaatcc ctacgccgcg atcgcggccg ggatcgcgac cctgtggggc ccggcgcacg
     3841 gcggcgccaa cgaggccgtg ctgcagatgc tcgagaagat cggctcggtg gacaacattc
     3901 cgaagttcat cgccaaggca aaggaccggg acgacccctt ccggctgatg ggcttcggcc
     3961 atcgcgtcta caagaacttc gatccgcgcg ccaagctgat tcgcaccatg tgccacaagg
     4021 tgctgaaccg tctcggccac accgactcgc cgctgttcga actggccttg cggctggagg
     4081 agatcgcgct cgaggacgag tacttcatcg agcgcaagct gtacccgaac gtcgacttct
     4141 attcgggcat catctaccgg gcgatcggca tcccgacctc catgtttacg gtgatgtttg
     4201 cgatcgcgcg gaccgtcggc tgggtggctc actggcagga aatgatcacc gatccgcagc
     4261 ggcgcatcgg gcgcccccgc cagctttaca cgggcgccgg caccagggac tacacgccga
     4321 tcgagcgccg cggggcgtag tcgcgccacg ggcgccggcg tgccggccgg gcttcagtcc
     4381 cggcccgacg gcctgaaggc ggcgtcgtcc gccgccgggc tcgcggcagc gagcagcgcc
     4441 tccacctctt cgcgccgcgc ccggcgcatc tggccgccgt tgacgggaca gacaggcgcc
     4501 tcgcgcagcc catgttcggg gaataccgtc acttccaccg gtacgccgtg cacctcgaac
     4561 tcgaagctcg ggaagctggc gtactcgccg ccggccaggc ggacgcggcg ctcgccactg
     4621 cgataacggc cgccgcgctc gatgagcagg aaggccactt cttccggcgt cgcggcgaac
     4681 aggtgcaggg agacgtcagt gtgcagcgtg gcggtgccgg cgagcaccgg gcccaccagc
     4741 cggggattga agccctgcag caggcgcatg gcgtccagcg cggcctcgcg cagcacgcgc
     4801 aggttgtcgt ggtgggcggc cccgccgaac aggcgctgct gcgccgccag ggcatccgct
     4861 atttcctggt tggcgggcag cgcgtgacgg tcgacgatgc cgagccgctc cgcggccttg
     4921 cgcttggcca gcaggaaatc ccggacgccc tgttcgcgca tgatccgggc ggcttcctcg
     4981 gccacccgga tgcgcagttc gcccgctcgt tgtatcgcac gtttcggcat gctgctggcc
     5041 gccgttcaga acagcgggtc ctcctcgctg ctgccgtccg gatagcgctc ctcaccgccc
     5101 tccggcggcg ggggcacttc accgacgcgg aacacctcga agatcgcatc cggttcgccg
     5161 gcgcgcgcca gccggccggt gtcggccgac acgcgcaccg tgaccagccc gggcggttcc
     5221 gccggccggc tgctgcgcat gccctcgagc gcgccgcgca tgaatgccat ccagaccggc
     5281 agcgcggtgg tcgcgccggt ctcgccgcga cccagcggcc gctcctggtc gaagcccacc
     5341 catgcggtgg ccaccaggtc accgttgtag cccgcgaacc aggtgtcgcg cccgtcgttg
     5401 gtggtgccgg tcttgccggc gaggtcgttg cggccgagct cgcgcgccct ggccccggtg
     5461 ccacgccgca ccacgtcctc cagcatgctg gaaatgatcc aggcgttacc cgcatcgatg
     5521 gcctgcggcg gctgggtcga agcggcgtcc gccggcgttg cttcgcctga ctcgtcgttg
     5581 gcggcggccg gctcgcgcgc aaactccggc agtctgccgt actcgcactc ggcgcaggcc
     5641 cagggcggct cggcctggtg cagcagctcg tcgtcgggtc cgtaaatccg gtcgatgaaa
     5701 tagggcgcaa tccggaagcc gccgttggcg aatgcggcgt accccgccgt cagttccacc
     5761 ggcgttacgc cgccgctgcc cagcgccagc gtcaggttgc gcggcaagga atccgccggg
     5821 aagccgaagc ggctcacgta gttgaccgtg aacggtacgc cgatctgttc cagcacccgg
     5881 atggagacca ggttgcggga ttgcaccagc gcctcgcgca ggcgcatcgg cccgtagaaa
     5941 cggccggtgt agttctgcgg ccgccaggtg tcctcgagga ccgggtcgtc gtacaccacc
     6001 ggcgcgtcga tcatgatgct ggctgcggtg agtccgcgtt ccagcgccgc ggaatagacg
     6061 aagggcttga acgcggaacc cggctggcgt cgcgcctggg tcgcccggtt gaacttgctt
     6121 tgttcgaagt ccagcccacc gctgagcgcc accaccgcgc cgtcgcgggg gtcgagcgcc
     6181 accagcgccc cctgaaccgc cggcacctgg gacaatgccc aggcgtcgtc gcgccggtcg
     6241 agcaacagca cgtgcccggt cgccaccacg tcggccggcc gctccggcgc cggcccacgg
     6301 ttgttctcgt cgatgtagcg gcgcgcccag cgcatgccct cgaaatccag ccgcgcctcg
     6361 ccggcacccc gcacgaacac gtcggcgccc gtgtcgtcga ccgccagcac gagtgcgctg
     6421 cgcaggccgg ccggctccgg gtagctgtcg agcagtgcct cgacgtcctc cgcgccctcg
     6481 ggcagcgcct cgacctccgc caccggcccg cggtagccgt ggcgcaggtc atagctctcc
     6541 agcccctggc ggaccgcccg ctgggcggac ggttgcaacc ggctgtccac cgtcgtcacc
     6601 accgagtaac cggccgtgta cgcctccggg ccgtaacggt ccagcacttc ctggcgcgcg
     6661 atctccgcga cccagggcgc ccgcaggcgc acctgggcgc catggatccg cgacagcacg
     6721 ggctcggccg atgcggcctc catcgcctcg cgatcgattg cgccggtctc gaacaggcgg
     6781 cgcagcacgt aggcgcggcg ttgccgggcg cgttgcgggc tggtgacggg gttgtccagc
     6841 gacggcgccc gcggcaggcc cgcgatcagg gccatctcgg ccaggctgag ctcgccgacc
     6901 tgcttgccga agtacacctc ggccgcggcg ccgacgccat aggcgcgctg gccgaggaag
     6961 atcttgttca ggtagagatc gaggatttcc tccttcgaca gcaggtgctc gatgcgcagc
     7021 gccaggaaga tctcgcggac cttgcgcgaa acggtctttt cgcggctgag aaagaaattg
     7081 cgtgcgacct gcatggtgat cgtaccgccg ccctgcgccg gctcgccggt ggcgatgacc
     7141 gccagcgtcg cgcgcgtcag gccctgccag tcgacgcccg gatgctcgaa gaagcggtcg
     7201 tcctcggcgg ccaggaaagc ccgctgcaat tgttcgggca cctcgtcgat gcgcagtgga
     7261 atccggcgct gctcgccgaa ctcggccatc agcaggccgt cgcgcgagta gacccgcatc
     7321 ggcacctgca ggcggacgtc gcgcaacgac tccacgccgg gcaggctcgg cgccacatag
     7381 taataggcgc cgatcgcggc gagcgcgagg gcggtcagcg acatcgcaac cagggcgacg
     7441 atgccgtaga aaaggcgcag gagaactctc atcggacggg ggcagctggc ttgccttttg
     7501 ccaaggcgct cagcatatta ccacttcatg cgaacggccc cgaacagggc cgccggcggt
     7561 gcagggcgcg gcagaatggc caccgccgat cccctcttcg gagcgccgtg ggtgagcggt
     7621 ccgaaatatg tcaacccgtg accccgttca cggattcgcc ggcgctttct gctgcaatgt
     7681 cggcttccgg cttcgggcac gccggtgggc ggactggatg ggtccgttgc attagttaat
     7741 gtcgtagtct atcttcgcct tccaagcccc ggacgcaaaa ggaaattcaa agtgcttttc
     7801 gggagatcca acaagccact gctggggctg gatatcacga cttcctcggt gaagctcatc
     7861 gagctttcga agtccggaga cacttatcgc gtcgaggcct acgcggccga ggccacgccg
     7921 ctcaacgcgg tgaatgaaaa ggccatcgtc gacgctcagg tggtgggcga ggcggtgcgc
     7981 cgggcgctca agcgctcggg agcgcgttcc cgcgaggcgg cggtcgcgat cagcggtgat
     8041 gccgccatta ccaagatcat ccagatgccg gcgaacttca ccggcgccga actcgaaggc
     8101 caggtggaat tgcaggccga ccagtacatc cccttcccga tggacgaggt cagctacgac
     8161 ttcgaggtga tcgggtcgac cgagaaagac ccggacatga tcgacgtgct gctggtcgcg
     8221 acccgcaccg agaacgtcga gcagcgccag gccgcggtcg aggccgccgg cctgaagacg
     8281 cgcatcatgg acgtcgagcc gttcgcggtc gagaacgcct gcctgctgct gcgccaccag
     8341 atgctcgacg agggcatggg ccacaacgtc gcggtgatgg acttcggcgc cagcagcacc
     8401 accttcagcg tgctgcggga tctgaaggtc atctacacgc gcgacttcgc gttcggcggc
     8461 cagcagctca ccgaagagat catgcgcacc tacgggctga ccctcgagga ggccgggcgc
     8521 gccaagaaag agggcggtct gccgagcaac tacgagccgg aaattctcga gccgttcatc
     8581 gatgacatga cccagcaggt gagccgctcg ctgcagttct tcctggcttc aggcaccaac
     8641 cgcgagcagc cggagcagat cctgatctgc ggcggttgcg ccaacatccc cggcgtggcc
     8701 gacgtcatcg gcagccgcgt gggcatcccg acccagatcg gcaacccgct ggggatgatg
     8761 aaagtttcct ccaaggcccg ctcccagggc gtgttgaagg acgccaccgc gctcctgacg
     8821 gcctgcggcc tggcgctcag gagcttcgac tgatgcatcg cataaacctg cttccgtggc
     8881 gcgcggaact gcgcaaggaa cggcagaaga atttcgccgt cgcgaccgtt ggcgccatcg
     8941 tgctcgggct gctgatcatg tgggcggtga acaccatcct gcagggccgc atcgactacc
     9001 agaattcccg caaccagcgc ctgcagcagg aaatcgcggt gctggacgag cagatcaagg
     9061 agatccgcaa cctccggctg cgcaaggatc gactgctgct gcgcatggcc gccatcgatg
     9121 agttgcagcg ggccaggccg gaggtggcgc acctgttcga cgagatcgtc aaggtgatgc
     9181 cggacggcgt gtatctcacg gagttcaccc agaacggcag aagcctgctg ttcaagggcg
     9241 tcgccgaatc gagcaccagg gtctccgcac tgatgcgcaa catcgcgagt tcgcggagca
     9301 tggccgatcc gcgcctgcag gtggtgcagt cggggcagca gcagcgcggc cgctcggcgc
     9361 ccgcaccccg cgggcccggc cgccaggccg agttcgcgct ggctgcgcgc caggccggcg
     9421 gcgcggacga gggcgacgag gaggtgctgc agtgaagttt ttcgaccagc tccgctcgct
     9481 cgacacccag gacgtcgggc gctggccgcc gctgttccgc ggcttggcga tcggcatcat
     9541 cttcgtgctg gtggccgtcg gcgccaccta ctatttcgtg tggcgcgtga agatccccca
     9601 gctcgagcag gtgcagcgcc aggagcagga cctgcgcacc accttcgaga gccgccagcg
     9661 gctcgccgcc aatctcgagg cctaccggga ccagctcgag gaagtcgagc ggaccttcgg
     9721 cgcgatgctg cgccagctgc ccggcgagac tgaagttccc agcctgctgg tcgatatctc
     9781 ccagacgggc ctggcggccg gattggagga gaggctgttc cagccgatga gcgaagtcag
     9841 ccgcgaggcg tacagcgagt tgccgatcaa gatgcaggtc gtgggcacct atcacgagct
     9901 cggcaatttc gtcagcggcg tggtcgccct gccgcgcatc gtgacgctgc acgacgtggt
     9961 gctcaagccc gagcaggccg agcgcgggcg ggccaccgat gcgccgccga gcaaccgcct
    10021 ggtcatggac gtcaccgcca agacctatcg ttacctggag gatgagggcg caggccaatg
    10081 aacgcaccct cgcgcattct gctgttgacg gcgacgctgg ccctggcgct ggtcgcgagc
    10141 ggctgctcgc gcggcatgtc ggacctcgaa ggctacatcg ccgaagtcaa ggcgcgaccc
    10201 ggcggtcgca tcgagccgct gccggagatc aagacctacg agaccttccg ctacaacccg
    10261 ggcgacgcgc gttccccgtt cgtaccggac gttcccacgg ccggcagcgt ggcgggcggt
    10321 gtccggccgg atgcggaccg gcgccgggaa tacctcgaga gcttcccgct cgacacgctc
    10381 aacatggtag gtacggtcag catgggcggg cgcctgtacg gcctcgtaca gacctccgac
    10441 aagctcgtac accgcgtgct ggccggcaat tacatcggcc agaacgacgg ccgtatcgtt
    10501 tcaatcacca gttccgagat ccagttgctg gagatcatcc cggacggcct cggtggctac
    10561 atcgagcgtg cggcctccat cagcctgggt gacttatgaa gctcggggag atagcactca
    10621 tgatgcgcga tcaatggcga agcaacagga cagtcgggac cgggaccagg tcgcggctgg
    10681 gctggtggac cgtcgccgta ctgcccctgc tgtggtggtc cggtgcggcc tcggcgcagc
    10741 aggatctgca gctgaccggc ctcgatgtga gtaccctgcc cggcaaccgg gtggtgctcg
    10801 tcctgaccac cagcggtccc gcgcccgagc cgctcgcctt cacgatcgag gatccggcgc
    10861 gcatctcgct ggacctgccc ggcacgaaac tcggcgtggc cgagcggcgc cgtgacgtca
    10921 agcgcggcgt gctggacacg gtcatgcttg cggaagcgca gggccgcact cgcgtcgtgc
    10981 tcaacctgga cctgatggtg ccctatgaga ccgcggtcga cggcgaccgg atcacggtga
    11041 cgctcggcgg cccggcggcc ccgtcgcagg cggctcgcgc gagcgccttc ggccagccct
    11101 ccggcagtgt cgctgttgcc gcccccgcgg ccggtgcggc gatcaccggc gtcgacttcc
    11161 gccgcggcgc cgacggcgcc gggcgcgtgc tggtcaacct gagcgatccg tccgtcgcga
    11221 tcgatattcg cgaggacctc gaccggaccg tggtcgagtt ccgcaatgcc agcctcccgg
    11281 acaacctgct gcggcgtctc gacgtgctcg atttcgccac cccggtgcag accatcgacg
    11341 ccgtccgttt ccccggcggc gcgcggctgg tcatcaatgc cgccggcaac tacgagcagc
    11401 tggcctacca ggccgacaac cagttcagca tcgagttccg gccggtggcc gagcaggacc
    11461 cgagcgagta cgggctgttc gacgagcgca aggagtacac cggcgaggcc ctgacgctga
    11521 acttccagga catcgaggtc cgggccgtgc tgcagctgct ggccgagatc agcgggctca
    11581 acgtcgtggt gagcgacacc gtccgcggca acgtcacctt gcggctggac agcgtgccct
    11641 gggaccaggc gctcgacatc atcctcacga ccaagggcct ggacatgcgc caggtcggca
    11701 cggtggtgct ggtggcgccg gcggccgaga tcgccgcgcg cgagaagcag gagctcgagg
    11761 ccaagaagga catccaggag ctggcgccgc tgcggctcga gttcctgcag gtcaactatg
    11821 cgaaggcggc ggacctcgcc gtgctgctga ctgaaaccgc cgggcgtggc gtgggatccg
    11881 ggaccggcac ccgcaccatg ctgtccgagc gcggcaacgt cagcatcgac gagcgcacca
    11941 acacgctgct catcaacgac accgcggaac ggatcgccga catccggcgg ctggtcgcgg
    12001 cgctggacat cccggtgcgc caggtgctga tcgagtcgcg catcgtggtc gccaacgacg
    12061 acttcagccg cgaactgggc gtgcgcatgg gcggcaccgt ggtgcgcgag aacggcagcg
    12121 acgggctgat caccatcacc ggcagcggcc agggcaccga caccatcgtg ggatcggggc
    12181 tgaacaacct caaccagacc ggttccgcct tcccggtggc cctgccgagt ctgggcgacc
    12241 gctacaacgt caatctcccc gccaacgaac cggcgggccg gatcggactc gcgatcctgg
    12301 ggtctgatta cctggtcgac ctcgagttgt cggcggcgca ggccgagggc cgcggcgaga
    12361 tcgtctccag cccgcgggtc atcaccgcga accagaaaca ggccctgatc gagcagggcg
    12421 tcgagattcc ttaccaggag gccgcctcca gcggcgcgac cgcgacgcag ttcaagaagg
    12481 ccgtgctcag cctgacggtg acgccgcaga tcacgcctga cgaccgcatc atcatggatc
    12541 tcgaggtcac ccaggacacg gtcggccagt tcgtgcccag cgcgaccggc ggccaggtgc
    12601 ccagtatcga cacgcgccgg atcatcaccc aggtgctcgt gaacgacggc gaaacggtgg
    12661 tgctcggcgg catttatgag accgagcggc gcgatgccga ggacaaggtg ccgctgctgg
    12721 gcgacatccc cggcctgggc cggttgttca agcggaacag ttcggtatcc aacaagcgag
    12781 agctgctgat cttcgtgacg ccgaagatcc ttcgtgaagg cagcaacatc tattgaactc
    12841 ccacgagtgg acgcgagtga gccctaatat gcgcagcagc aaattcacac gtcgagtact
    12901 cgggttgccg gggctcctgg ccgccctttt cctcgtcggt tgcggcagcg agtcgacgat
    12961 cgtcggaccg ccgggcggcg ggacgcccga ttccccggtg gcgagcatca ccctgatcgc
    13021 cagcagcaac gtcctgccct cgggcggcgc ggccgtggtg actttcagcg tcctggtgcg
    13081 ggatgccaac aacaacgcgg tcaccggcgt gccggtgacc tactccgcgg actccgggct
    13141 gctgcaggtc attcccgacc cggacggcgt tgcgctgccg gccgccgaac tttcgaccgg
    13201 gggcgacccg accaaccggc ccatcacggt caccgccacc acgggctcgt tgtccagctc
    13261 cgtcacggtc aacgtgaccg gcaccgtgct gactctcgaa ggcccggaca acgtcatcca
    13321 gggcgacgaa gtgccgttca ccgcgcgcct gcagaattcc gccggccagg gcattgccgg
    13381 ccgcaccgtc accatcgagt cggccgcggg caacacgctg tccgctgact cgctcactac
    13441 caacagccag ggacaggtgt cgttcacggt gaccggcacc cagggcggtg ccgacacgat
    13501 ctcggtcagc gcgctcggcc tgaccgctac cgcgcccctg tcggtcagcg ccgacgacag
    13561 cgtggtgttc ctcgagccgg ccgccggaac ggagattccg ctcaacgtcg cgcagccggt
    13621 gaccgtcgag tggcgcgtca acaacgtgcc gcaggcgggc cagaccatca acttcagcac
    13681 cacgcgcggc tcgctgtcgg ccctgagcgc ggtgctcgac gccgcgggcc aggccaccgt
    13741 gaacctcagc gccacgacgg ccggtcccgc cacgctcacc gcgcgggtcg gggcggcggg
    13801 gccgcaaacg cagcgctcca tcgagttcgt ggcgctcacg cccgccagca tcgtggtcca
    13861 ggccagccgc ttcacggtcg gcccgggcga gcagagcacc atcgtcgcga cggtgcgcga
    13921 tgcggccaac aacccggtca agaacaaggt cgtgcagttc cagatcgagg acgtcaccgg
    13981 cggcagcctg tccgtcggta ccgccatcac cggcagcgcc ggtaccgcgc agaccgtcta
    14041 cacgggcggc gagaccatca gcggcagcgg cgacgtgacc atcacggcca tcgtgcagga
    14101 gaacccggca ctgaccgcga cgctcgggct gacggtggcc cgccgccagg tggacatcag
    14161 catcggcacg ggcaacgagc tgttcgagcc caacacggca cagtacgaac gtgagttcct
    14221 cgtccaggtg accgacagcc agggggtggg tgtcgcggat gcagccgtgc agctgtcggt
    14281 gcgttccaag ctttaccgca agggcttcta cgtcgaaggc gccaacgacc gctgggtggt
    14341 cggcccgggc gcgatcctgt gcccggacga ggacctcaac aacaacggcc agctgaacgc
    14401 caacgaggac ttcaacggct cgggctcgat cgaggcgggc aacgtcgtgt cggtcacgcc
    14461 gggtgacgtg gtcacggacg agagcggcac cgcgatcttc cgcatccgct atgcgcagga
    14521 attcggcaac tgggtgatcg tgacgattac cgcgcgcgcg gcggtgcagg gcaccgagtt
    14581 cgaggatcgc agcgatcatg cgctggagat cacggccgcg gacgccaacg tcaaccagag
    14641 cccgccgggc gggctcatca gccgcttcgg cggcggcgac ctcgacccga atccggaccc
    14701 gacggacgag gggctcactg tccccgagta cgcgacccgg ctggccgagc tcggcttcgc
    14761 cggcggtcgg cctctgggtt gccgcacgga agactagtcg cacgctgttg cggttatcat
    14821 tacggggccg gcgcaagccg gcctcgtgtt atggggcgac ggacaggcca tggcgctacc
    14881 caacatcttc ctggtcgggc cgatgggcgc cggcaagtca gcggtgggga agcagctggc
    14941 caggatgctg cggcgccagt ttcacgacag cgatgccgag atcgaggcgc gcactggcgt
    15001 ggatatccct ttcatattcg agaaggaggg cgaggtgggc tttcggcgcc gcgaacgcga
    15061 agtgatcgag gcgctgacct tgcttgacgg catcgtgctg gcgactggcg gcggcgcggt
    15121 gctggacccg gccaaccgtc atgcgctggc gtcgcgcggc ctggtcgtct accttgaggc
    15181 gtccgtggac cagcagctcg agcgcacgcg gctttccagc caccgcccgc tactcgacac
    15241 ggtcgatccg gccgagcgcc tggcgcggct cctgcaggag cgggcgccgc tgtaccgtga
    15301 agtggcggtc ctggcgatcc cgacggacgg tcggctggtg agggaagtcg cccaggagat
    15361 caagcgcacc ctggcggcat tgagcgagga agactgaggc aatccatgca tgcgttgaac
    15421 gtaccgctag gggacaggac ttacccggtg ctgatcggac gcggcctgct cggcagggcc
    15481 gaggtcctgg cgccgtatct cgacgggcga caggtgctcg tggtgaccaa cgagatcgtc
    15541 gcgccgttgt ggctcaagcc gttgcgcgcg gcgctggccg attacgagct cgcggtgcat
    15601 gtcctgcccg acggcgagga gcagaagtcg ctcgagacgc tgggattgtt gttcgacgtg
    15661 ctggcggagc accgcttcaa tcgtgacgcc acggtgctgg cgctgggtgg cggcgtcatc
    15721 ggcgacgtgg ccggcttcac cgcggcctgc tggcagcgcg tcacggcctt cgtgcagttg
    15781 ccgaccacgc tgctcgccca ggtcgacgcg gcggtcggcg gcaagaccgc cgtcaacatt
    15841 cccgccggca agaacctggt cggcgccttc caccagccga gcgcggtgat tgccgacgtc
    15901 gccacgctcg gcacgctcgg ccgcaaggag tattgcgccg gtctcggcga agtcgtgaag
    15961 tacgggctcg gcctggacag cggcctgttc gagtggctgg agcgggatct cgatgcggtc
    16021 atcgatcgcg agcccgaggc gctcgagcgg atggtgtact ggtgttgcgc cctgaaggcc
    16081 gccgtggtcg ccgaggacga gcgcgagcag ggccggcgcg cgctgctcaa cctcggccac
    16141 acctttgcgc acgccatcga ggcggcgacg gcctttggcg cctggctgca cggcgaagcg
    16201 gtggcggtcg gcctggtgat ggcgtcggag ctggccgtcc gcatgggaat gctcgatcgc
    16261 gacatcgcgg cgcgggtgga ggcgctgatc gccgcggccg gactgccgac ccggccgcca
    16321 cccgtagggg ccgagcggct gaaggccttc atgcagctcg acaagaagat cgccggcggc
    16381 cggctgcgct tcgtgctgcc cgacgggctc ggctcctgcg tcgtgcgcgc ggacgtgccg
    16441 gaggcggagc tcgatgcggt gctggcgatg gccgcgccgc ggtgagtgaa gcgccggcgc
    16501 cctgggccgc ctgcgaaacc cgctcgcccg gccggcgcta cccggagccg ccccctcgat
    16561 atcgcggcga gtaccatcgc gaccgcgacc ggatcattca ttccaccgcg ttccggcggc
    16621 tggtctacaa gacccaggtg ttcgttaacc acgagggcga cctgtaccgg acgcgcctga
    16681 cgcactcgct cgaggtcgcc cagattgcgc gcaccgtggc gggcgcgctg gggctgaaca
    16741 cctacctgac ggaagcgatc tgcctggccc acgacctggg ccacacggcc ttcgggcacg
    16801 ccgggcagga agcgttgaac cgctgcatgg cggagtacgg cggcttcgag cacaacctgc
    16861 aatccctgcg cgtcgtcgac gagctggagg aaaagtacgc ggagtttccg ggcctgaacc
    16921 tgatgttcga aacccgcgag ggcatcctca agcattgctc ggcgc
//
LOCUS       DAOMPG010000023        16707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1515_length_16707_cov_4.531408, whole genome shotgun sequence.
ACCESSION   DAOMPG010000023 DAOMPG010000000
VERSION     DAOMPG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16707
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_16707_cov_4.531408"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLS34_02210"
     CDS             complement(<1..322)
                     /locus_tag="PLS34_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HRP86320.1"
                     /translation="MSDAAAAWLRLSLTPGVPAEAARAAAAHPGGLEGLLAAGKGALM
                     GIGIPADAAQVLAGPAPDWLPKALDWLAAPDHDLLTLADADYPPLLRGISAPPVALFL
                     RGDRA"
     gene            complement(395..1543)
                     /locus_tag="PLS34_02215"
     CDS             complement(395..1543)
                     /locus_tag="PLS34_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRP86321.1"
                     /translation="MSKPIYRPTRIRLLSGLAAALLAAGCASTPEATPPPEATPAPAA
                     RPAATPPPAAPVAAAPAAPVRLNPQHPDRYVVKKGDTLWDISAMFLRDPWFWPEIWYA
                     NPQVRNPHLIYPGDVLTLVYMDGRPRIILERGETGARADRLEPRVREEALEDAIFTIP
                     YDRIAAFLSRPTVLDRETVDNAPYILTSRRGHLVHGSNTEVYVRGADFVEQDDFSVIH
                     VGAPLRDPDDNRLLGYEGIYVGEGQIVRAGDPATLYLRQTHREALNGDKLLPVETDFP
                     LHFVPRAPDEEIDGRIINVLDGVSLVSSYQIVTLNRGAKHGLEPGHVLAVWQAGENIR
                     DRIRGGRAQLPDEYAGFLMVFRSYEEISYGLIMRATNEIRVLDKVRNP"
     gene            1746..2273
                     /gene="def"
                     /locus_tag="PLS34_02220"
     CDS             1746..2273
                     /gene="def"
                     /locus_tag="PLS34_02220"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRP86322.1"
                     /translation="MAILNILEYPDPRLRTKAEPVAVVDDELRQLAADMLETMYAAPG
                     IGLAATQVNVHRRMLVADISDKQDAPHIFINPEILERRGTEVMEEGCLSVPGFYESVT
                     RSDWIRVKALGLDGQAFEKEVDGLLAVCIQHEIDHLDGKLFVDYLSELKRQRIRKRLE
                     KERKQREPSPAARAL"
     gene            2284..3213
                     /gene="fmt"
                     /locus_tag="PLS34_02225"
     CDS             2284..3213
                     /gene="fmt"
                     /locus_tag="PLS34_02225"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRP86323.1"
                     /translation="MSRLVFAGSSDFAVPTLAALLAGGWEIAGVLTQPDRPAGRRRRL
                     TPTAVKELAVAHGLLVQTPATLRAPAAVAAVAALRPEVMVVVDYGLILPPAVLGLPPA
                     GCINGHASLLPRWRGAAPIQHAILAGDAVTGVSVMLMDEGLDTGPVYKMQSTEIGVAE
                     TGGELRARLAQLCAQLLAETLAEILTGKLEPQQQDHARATYAGKLDPAAAELHWRGSA
                     EVLARQVRAFSPRPGAWTTWRGERLKLHRAVSLPAREGEPGQVWRAGPEGVDVAAADG
                     SVRVLELQPPGGRIMSARDFLNGRAVLGERLGA"
     gene            3210..4538
                     /gene="rsmB"
                     /locus_tag="PLS34_02230"
     CDS             3210..4538
                     /gene="rsmB"
                     /locus_tag="PLS34_02230"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRP86324.1"
                     /translation="MNAGGEPRAAAGRAVAAVARRGRSLDDALGKAVAGLHGADRSLA
                     AQLAYGTLRAWPRLDRWVDLLLARPLPARDEDVHDLLAVGLYQLEDTRVPDHAAVAAT
                     VEAVRLLGRHQYAGLVNACLRRWLRERETLKVRTARVPEVIHAHPRWWLDALAADWPK
                     DWESIVAAANQPGPMWVRVNRLHGTRAAWHERLLATGATAAPWEPAPDALLLASPMDV
                     EALPGFSAGDVSVQDAAAQLAAGLLDPQPGQRVLDACAAPGGKAAHLLERCPGIDLTA
                     LDIAPARLERVRENLARLGLAARVQAGDAGDPAGWWDGRPYDRILLDAPCTGSGVVRR
                     HPDIKLLRRPEDVAVMAARQRALLEALWPLLAPGGRLLYATCSVFRAENAGCVTAFLA
                     ANPEARAVDLGEPGWGRRAGPGRQVLTGEAAMDGFYYACLTRTAQAQSQA"
     gene            4543..5133
                     /locus_tag="PLS34_02235"
     CDS             4543..5133
                     /locus_tag="PLS34_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRP86325.1"
                     /translation="MQQGARYFDSPGWRAAAAGLFAALLLQPGLARADEGVEVRNAST
                     RLQAGVWFLDADIAYGLNATALEALDSGLVLDAELTIRLTQRRRIIWDPVFAELKQRY
                     SLQFHALTERYILRNLNSGEQETYGSLEAALAALGTVRSLPVIDDALLSRGERYYVEI
                     RAVVDIKELGGPLALITFLWNDWRIKGDWVRWRLNR"
     gene            5115..7343
                     /locus_tag="PLS34_02240"
     CDS             5115..7343
                     /locus_tag="PLS34_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP86326.1"
                     /translation="MATQSLKRWGLASLALLGLSMLVIALLFLASTVENADRFGEIYN
                     WIFLLNLAGASILLVLIVSNLVRLVREYRSQVPGSRLASRMVAIFVGLAVLPLGLVYY
                     FSVQFISRGIDTWFDVNVEAGLGDALDLSRATLESRMREHMEATRQIANSLRGRDDIE
                     LALRLESLRRNAGAIELTVVGQSARIIALSSERSLAELPVSPTEEVMLQIRQGRPFVS
                     LDPVRDGFVVRTAIAIPGGNPLDELRLLHALFPVPERVGQLANSVQEAYAQYRELSFL
                     RGPFTRSLTLTLTLVLLLSLLMAVWGAFFFARKLTEPIQTLVAGTRAVAKGDFDTRLP
                     MPARDEMGFLVHSFNDMMTRLAGARELARQSQQAVESQRAYLAVILGRLSTGVVSLER
                     DWRIKTANEAAGAILGVDLEGAIGRPLAEVAEDRPLLQQFIGVGREHVEAGEFEWREQ
                     IVLRNEAGRRVLMCACSGLPGEDGEPDGFVVVFDDITALLQAQRDAAWGEVARRLAHE
                     IKNPLTPIQLSAERLRRRFLEQMSDDDAMVLERATRTIVQQVEAMKEMVNAFSDYARA
                     PDMEIARVDLNQLVAEVADLYRVREIPVTVQLSLDPGVTAIEADSGRVRQILHNLIRN
                     AVEALEGHDDAVVRITTRGTDEFDLRAVFIMVEDNGPGIPPDSVDRIFDPYVTSKPKG
                     TGLGLAIVKKLVEEHGGWVEASNRAEGGVRVTIALPADEDARSAMLRHEVRASELRRA
                     RA"
     gene            7340..8710
                     /locus_tag="PLS34_02245"
     CDS             7340..8710
                     /locus_tag="PLS34_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRP86327.1"
                     /translation="MSTGRILVVDDEVDIRQLLDEILSEEGYEVSSAANAAQARRQRE
                     AVRPDLVLLDIWMPDADGISLLREWSTSEDLRPCPVVMMSGHGTVETAVEATRLGAFD
                     FIEKPVSLAKLLRTVERALESGRAPGRAARQLVPATVVPVGRSRLMQALREQSERVAA
                     HSTPVLVTGEPGTGRESFARYIHSLGRQARGPFVRMVASALQDADAEAALFGRVVDGG
                     TEPGFLERARGGTLFINELSDLCPMAQRLLLGALENGRFTPVGDAEPRAMDLRIISSA
                     PVGFEHVPATAAFRRELLTQLAVVVLKVPPLREYSEDVPELLRHYMDKLVDTDGLPFR
                     KLSVAAQNRLRNYPWPGNVRELRNLVHRLLIMGGEEEIGLEEVEQALGEQLSPDEGLL
                     SQDLMALPLREAREQFERAYLQQQLQLCEGKVGQLAKRVGMERTHLYRKLRALGIDFR
                     SSGTDD"
     gene            complement(8721..9281)
                     /locus_tag="PLS34_02250"
     CDS             complement(8721..9281)
                     /locus_tag="PLS34_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP86328.1"
                     /translation="MPRDYKRRRPRKPATPGWAWLLTGLALGLSVALAVHLRHTSSTA
                     PDEPVAARVAKPASQQADEATEIEPAPASGFDFYTMLPGLEVVVPEEEVRPRGAGPAA
                     PAPALAPGRYWIQAGSFRRYQDADRRKAELALLGLGSSIQAVTIEGQTWHRVRIGPLP
                     GAAEAEATRRRLADANIGALAVRERD"
     gene            complement(9284..11035)
                     /gene="argS"
                     /locus_tag="PLS34_02255"
     CDS             complement(9284..11035)
                     /gene="argS"
                     /locus_tag="PLS34_02255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRP86329.1"
                     /translation="MKKQIEGLLADALATLCEQLSLTDVAAARVERARDPRHGDFATN
                     AALLLAKPAGMNPRELADRIVGLLPASELVESVSIAGPGFINFRLSRRAFERETAGVL
                     AAGAAYGRSDVGAGQRVLVEFVSANPTGPLHVGHGRHAAYGASVANLLEATGHLVHRE
                     YYVNDAGRQMDILAASVWLRYLEALGEEFAFPANGYRGGYVQPIADKLVALEHARLRR
                     PAAEVFTGLPPDAPEGDKDDYIDAVVARMRELLGDADYRAVHKLGLDEILGDIRDDLA
                     EFGVTYDEWFSERSLTDLGLVAECIDRLKADGHAYERDGALWFRASALGDEKDRVVVR
                     DNGQTTYIASDIAYHLGKRQRGHDHLLDILGADHHGYVARVRAGLEAMGEPGDSLEVR
                     LVQFAILYRGGEKVQMSTRSGEFVTLRELREEVGNDAARLFYVLRSNDQHLDFDLELA
                     KSRSNENPVYYIQYAHARVRSVFRQLVEKGLEWSADEAVTHLRLLKTDQERVLMASLG
                     RFPEVVELAAANRAPQNIVHYLRELAQDFHTWYNAETFLVDEAPLRSARLALAEATRV
                     VIANGLALVGVSAPESM"
     gene            complement(11124..13283)
                     /locus_tag="PLS34_02260"
     CDS             complement(11124..13283)
                     /locus_tag="PLS34_02260"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRP86330.1"
                     /translation="MTILKVAVDAPLDTLFDYPAPDSGPPPAPGVRVRVPFGRRRPVG
                     LVMAHAAESDLPDERLKPVLEALDAAPLFDTALLKLLDWTARYYHEPPGPVVATALPA
                     PLRRGKPLPEPPARVVARGPAPEAMKKRAPKQAALLDLLAAAPRRTDDPALPPGWRAG
                     VARLAARGLVEIEPEPAAAAWTAALAGPTLNAAQAAAAAAIIGDLGRFSPRLLFGVTG
                     SGKTEVYLVAAAECLRRGLQVLVLVPEIGLTPQLVQRFAARLAAPVAVLHSGLPDGER
                     MTAWADARSGRAAVVIGTRSAVFVPLARPGLIIVDEEHDPSLRQHEGMRYSGRDVAVM
                     RASLADIPIVLGSATPSLETLKHARDGRYALSRLPERPGASTHPRVSVIDLRRHPATE
                     GLSAPLLGAMRAHLDAGGQAMLFLNRRGYAPALFCSSCGWAADCRHCDARYTLHRRIG
                     RLRCHHCGDDTALPAGCPACGADLRPAGEGTERVEDALARHFPGVTIARIDRDATRRR
                     GSVERILDDVRDGRTRILVGTQMMAKGHDFPGVTLVGVINADQGLFGTDFRAGERLAQ
                     TLVQVSGRAGRADRPGEVLVQTAYPEHPLLQRLLGGGYEAFAEAALEERRAAGWPPFS
                     HLALLRAEAARPGAALRFLAACRARLHPSATLVVLGPAPAPMEKRSGRWRAQLLLQSG
                     ARPTLHAALAELKAIAPDLPESRSVRWFFEVDPQDLF"
     gene            complement(13665..15044)
                     /locus_tag="PLS34_02265"
     CDS             complement(13665..15044)
                     /locus_tag="PLS34_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRP86331.1"
                     /translation="MGFATFMIKASYFIAALLFIIGIKRMSSPRTARGGILWAGWGMV
                     LATVVTFFWPGMSAVNIGLIIIAILLGGGLAWWTGKKVAMTDMPQMIALYNGMGGGAA
                     GAIAAVTLYKGAPDSLTVGFLAVLGGLIGSVSFSGSLIAFAKLQGWMTKPIRFKSINQ
                     LNAALLLSAFIIGMFLLGNYSAHGTLVTLFFVLALVAGIMLTSPIGGADMPVVISLYN
                     ALTGLAVAFKGFVLDNEAMIIAGTVVGAAGTLLTQLMAKAMNRSLASVLFSAFGDESV
                     DSGEVSGSMKPIEASDAGIMMAFADRVVIIPGYGMAVAQAQHKVWEMAQLLRERGVKV
                     SFAIHPVAGRMPGHMNVLLAEAGVPYDLIFDLDEINADFEKTDVALVIGANDVVNPVA
                     RTDPSSPIYGMPILNADKAKNVIVIKRGKGKGFSGIENRLFYLDNTRMLYGDGQAAVS
                     ELIHAIKAG"
     gene            complement(15044..15346)
                     /locus_tag="PLS34_02270"
     CDS             complement(15044..15346)
                     /locus_tag="PLS34_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRP86332.1"
                     /translation="MIGGIEGFFIALYIFMLAAFTGYEIIARVPVILHTPLMSGSNFV
                     HGIVLVGCMVALGKADSTLGQVIGFIGVVLGAGNAVGGYVVTERMLEMFKSSGERK"
     gene            complement(15343..16485)
                     /locus_tag="PLS34_02275"
     CDS             complement(15343..16485)
                     /locus_tag="PLS34_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRP86333.1"
                     /translation="MALVIGALRETAAGETRSPLVPEVAKKFAALGATICAEHRIAEA
                     ARIPDSRFEGVEFVGREEVVSRADVLIAVNPPPLEVAAGLRENAVLIGLLQPHRSDEL
                     VGLLRDRRITSFSMELVPRISRAQSMDALSSQAAVAGYRAVLIGAMHLDKFFPMLTTA
                     AGTIRPAKVLVIGAGVAGLQAIATAKRLGAIVEGYDVRSATREQVQSLGAKFVDTGIS
                     AEGTGGYARELTDEEKAKQAGILADHVAAADVVITTAAIPGRTAPRIIDAETVGRMQP
                     GSVIVDLAAETGGNCALTKAGEVVDASGVQVVGPVNLPAAVGRHSSEMYAKNLFNFLS
                     PAISEGTLAVDWEDEVFAGSVLTHDGRIGHAPTRERIEGASGGQST"
BASE COUNT         2464 a         5704 c         5997 g         2542 t
ORIGIN      
        1 gcgcccggtc gccgcgcagg aacagtgcga ccggcggcgc ggagatgccc ctgagcagcg
       61 gtggatagtc cgcatcggcc agcgtcagca ggtcgtgatc cggcgcggcc agccagtcga
      121 gcgccttggg cagccagtcg ggcgcgggtc ccgccaggac ctgggcggcg tccgcgggaa
      181 tcccgatgcc catcaacgcg cccttgccgg ccgccagcag gccctccagc ccgcccgggt
      241 gggccgctgc ggcgcgcgcg gcctccgccg gcacgcccgg cgtcagggac aggcgcagcc
      301 aggcagccgc tgcatcgctc atcgctggct cctcaaagca cgacgccggc agatgccggc
      361 gtcgtgggaa ggacggggca gacgcctggc gacgtcaggg attgcgcacc ttgtcgagca
      421 cgcgaatctc gttggtcgcc cgcatgatga gcccgtagct gatttcctca tagctgcgaa
      481 aaaccatgag aaagcccgca tattcgtcgg gcagctgggc gcgcccgccg cggatgcggt
      541 cgcgaatgtt ctcgccggcc tgccacaccg ccagcacgtg gcccggctcg aggccgtgct
      601 tcgcgccgcg gttcagggtg acgatctggt agctgctgac cagcgagacg ccgtccagca
      661 cattgatgat gcggccgtcg atctcctcgt ccggggcgcg gggaacgaag tgcagcggga
      721 agtcggtctc caccggcagc agcttgtcgc cgttgagcgc ctcgcggtgg gtctggcgca
      781 ggtacagggt cgcgggatcg ccggcgcgca cgatctggcc ctcgccgacg tagattccct
      841 cgtagccgag caggcggttg tcgtccgggt cgcgcagcgg cgcgccgacg tggatgacac
      901 tgaagtcgtc ctgctcgacg aagtcggcgc cgcggacgta gacctcggtg ttcgagccgt
      961 gcaccaggtg gccgcgccgg ctggtcagga tgtagggcgc gttgtcgacg gtttcacggt
     1021 ccagcactgt cgggcggctc aggaaggcgg cgatccggtc gtaggggatg gtgaagatcg
     1081 cgtcttcgag ggcctcctcg cggacccggg gctcgagccg gtcggcgcgc gcgccggtct
     1141 caccgcgctc caggatgatg cgcggccggc cgtccatgta caccagcgtc agcacgtcgc
     1201 cgggatagat caggtgcgga ttccggacct gcgggttggc gtaccagatc tccggccaga
     1261 accagggatc acgcaggaac atcgccgaga tgtcccacag cgtgtcgccc ttcttgacga
     1321 catagcgatc cggatgctgg ggattgagcc ggacgggcgc cgccggtgcg gccgccacgg
     1381 gcgcggcggg gggcggtgtc gccgccgggc gcgcggcagg ggccggcgtc gcttccgggg
     1441 gcggtgttgc ttcgggggtg ctcgcgcacc cggcagccag cagcgcggcg gcaaggccgc
     1501 tcagcaggcg aattcgggtc gggcggtaga tcggcttgga catggctggc ctgactccat
     1561 gctgtccgtt agttgagaag gggctgctgc ggcttggtat aatcagccaa gccttggccg
     1621 ccatcgcccg gacgccggtt ttcccggcgc ctttgcgtga gcggcaagtt agcacggcgg
     1681 acttgaaatg tcatactgtt agccgcactt tgtggcgtac tacacgagga accggcaagg
     1741 ccctcatggc gatcctgaac atacttgagt atcccgaccc gcgactccgc accaaggcgg
     1801 agccggtggc tgtcgtggac gacgagctcc ggcaactggc cgcggacatg ctcgagacca
     1861 tgtacgcggc cccgggcatc ggcctcgccg caacgcaggt caatgtccat cgtcgcatgc
     1921 tggtcgcaga tatctcggat aaacaggacg caccgcacat ttttatcaat cctgagatcc
     1981 tcgagcgccg cggcaccgag gtcatggagg agggctgcct gtcggtgccg ggcttctacg
     2041 agtcggtgac ccgctcggac tggatccggg tcaaggccct cgggctggac ggccaggcct
     2101 tcgagaagga ggtcgacggg ctgctcgccg tctgtatcca gcacgagatc gatcatctgg
     2161 acggcaagct gttcgtcgac tacctgtccg aactcaagcg gcagcgcatt cgcaagcgtc
     2221 tcgagaaaga gcgcaagcag agagagccca gccccgcagc acgcgcgttg taggccctgg
     2281 cgcatgtccc gcctggtgtt cgcgggcagt tccgatttcg ccgtgcccac gctggccgca
     2341 ctgctggccg gcggctggga gatcgccggc gtgctgaccc agccggaccg gccggccggc
     2401 cgccgtcgcc ggctgacacc caccgcggtc aaggaacttg ccgtcgcgca cggcctcctc
     2461 gtgcagacgc ccgccacgct ccgggcgcca gcggcggtgg ccgccgtcgc agcgctccga
     2521 ccagaggtca tggtggtggt ggactatggc ctgatactgc cccccgcggt gctcggcctg
     2581 ccgccggccg gctgcatcaa cggccacgcc tcgctgctgc cgaggtggcg cggcgcagcc
     2641 ccgatccagc atgccatcct cgccggtgac gcggtcaccg gcgtgtccgt aatgctgatg
     2701 gatgaagggc tggacacggg tccggtatac aaaatgcagt ccacggaaat tggcgtcgcg
     2761 gaaaccggcg gcgagctgcg cgcccgcctg gcacagctgt gcgcgcagct gctggcggag
     2821 acccttgcgg agattctcac gggcaagctc gagccgcagc agcaggacca cgcccgggcc
     2881 acctacgcgg gcaagctgga cccggccgcg gcggagctgc actggcgcgg gtcggcggag
     2941 gtcctggcgc ggcaagtgcg ggccttctcg ccccgaccgg gcgcctggac gacctggcgg
     3001 ggcgagcgac tgaagctgca tcgggccgtg tcgctgcctg cccgggaagg cgaaccgggg
     3061 caggtctggc gggcgggtcc cgaaggggtg gacgtggcgg ccgctgacgg cagcgtgcgg
     3121 gtgctcgagc tgcagccgcc cggcgggcgg atcatgagcg cacgcgattt cctcaatggc
     3181 cgggcggtgc tcggcgagcg gctcggcgcg tgaacgccgg cggcgagcca cgcgccgcgg
     3241 ccggccgggc ggtcgcggcg gtcgcacgcc gtggccgctc gctcgacgat gcgctcggca
     3301 aggccgtcgc gggactgcac ggcgccgacc gctcgctggc ggcccaactt gcctatggca
     3361 ccttgcgggc ctggccgcgg ctcgaccgct gggtggacct gttgctggcg cggccgttgc
     3421 cggcgcgcga cgaggacgtg cacgacctgc tcgcggtcgg cctgtaccag ctggaggaca
     3481 cccgtgtgcc cgaccatgcc gccgtcgcgg ccacggtcga ggccgtccgc ctgctcggtc
     3541 gccaccagta cgccggcctg gtgaacgcct gcctgcgtcg ctggctgcgc gaacgcgaga
     3601 ccctgaaggt gcggaccgcg cgggttccgg aggtcatcca cgcccatccg cgctggtggc
     3661 tggacgcgct ggcggccgac tggccgaagg actgggagtc catcgtcgcg gccgccaacc
     3721 agccgggacc gatgtgggtg agggtgaatc ggctccatgg cacgcgcgcg gcctggcacg
     3781 agcgcctgct ggcgacgggc gcgacagcag ccccctggga accggctccg gacgccctgc
     3841 tgctcgcctc gcccatggac gtcgaggccc tgcccggctt ctcggccggc gacgtgtcag
     3901 tgcaggacgc cgcggcgcag ttggccgccg gcctgctcga cccgcaaccg ggccagcgtg
     3961 tgctcgacgc ctgcgccgcg cccggcggca aggcggccca cttgctggaa cgctgcccgg
     4021 ggatcgacct gacggcgctc gacatcgcgc ccgcgcgcct cgagcgcgtg cgcgaaaacc
     4081 tcgcgcggct gggactggcg gcgcgagtcc aggcgggcga tgccggggac ccggccggct
     4141 ggtgggacgg ccggccctat gaccgcattc tgctggacgc gccgtgcacc ggcagcggcg
     4201 tggtgcggcg ccaccctgac atcaagctgc tgcgccgccc cgaggatgtt gcggtgatgg
     4261 cggcgcggca gcgggcgctt ctcgaggcgc tctggccgct gctcgcgcct ggcggccgcc
     4321 tgctgtacgc gacgtgctcg gtgttccgcg ccgagaacgc gggctgcgtg acggcgttcc
     4381 tcgcggcgaa tccggaggcg cgggcggttg acctgggcga gccgggctgg ggccgccggg
     4441 ccggccccgg gcggcaggtg ctgaccggtg aggcggcgat ggacgggttt tattatgctt
     4501 gtctgacccg gaccgcccag gcacagagtc aggcatagag tgatgcagca aggagcccga
     4561 tatttcgaca gccccggatg gcgcgccgcc gccgcggggt tgtttgctgc gctcctgctg
     4621 cagccggggc tcgcgcgcgc cgatgaaggc gtcgaggtcc gcaatgcctc gacccggctg
     4681 caggccgggg tgtggttcct ggacgccgac atcgcctacg ggctgaacgc gacggcgctc
     4741 gaggcgctgg acagcggcct ggtgctggac gcagagctca ccatccggct gacgcagcgc
     4801 cgtcgcatca tctgggaccc ggtattcgcc gagctcaagc agcgctactc cctgcagttt
     4861 catgcgctga ccgagcgcta cattctgcgc aacctcaaca gcggcgaaca ggagacctac
     4921 ggctcgctgg aggcggcgct ggccgcgctc ggcacggtgc ggtcgctgcc ggtgatcgac
     4981 gatgcgctgc tgagccgggg cgagcgctat tacgtggaga tccgggcggt ggtggacatc
     5041 aaggagcttg gcgggccgct cgcgctgatc accttcctgt ggaacgactg gcgcatcaag
     5101 ggcgactggg tccggtggcg actcaatcgc tgaagcgctg gggcctggcc agcctggccc
     5161 tgctgggcct gtcgatgctg gtcatcgccc tgttgttcct cgcctccaca gtcgagaacg
     5221 ccgatcggtt cggcgagatc tacaactgga ttttcctgct caatctcgcc ggcgcgtcca
     5281 tcctgctggt gctgatcgtg tccaacctgg tgcgcctggt gcgcgagtac cgcagccagg
     5341 tgccgggctc gcgcctcgcc tcgcgcatgg tggccatttt cgtcgggctg gcggtgctgc
     5401 cgctgggcct ggtgtattac ttctcggtcc agttcatcag ccgcggcatc gacacctggt
     5461 tcgacgtcaa cgtcgaggcc ggcctcggcg atgcgctcga cctgtcgcgg gcgacgctcg
     5521 agagccggat gcgcgagcac atggaggcca cgcgacagat cgcgaattcg ctccgcggcc
     5581 gtgacgacat cgagctcgca ttgcgactgg agtcgttgcg acggaacgcc ggcgccatcg
     5641 agctcacggt cgtgggccag agcgcacgca tcatcgcgct gtcctccgag cgcagcctgg
     5701 ccgagctgcc ggtgtcgccc accgaggagg tcatgctgca gatccgccag ggccggccgt
     5761 tcgtcagcct cgacccggtg cgcgacggct tcgtggtgcg caccgcgatc gccattcccg
     5821 gtggcaatcc gctggacgag ctgcggctgc tgcatgcgct gttcccggtg ccggaacgcg
     5881 tcgggcaact ggccaactcg gtgcaggagg cctatgcgca gtatcgcgag ctgtccttcc
     5941 tgcgcggccc gttcacgcgc agcctgaccc tgaccctgac cctggtgctg ctgctgtcgt
     6001 tgctgatggc ggtctggggc gccttctttt tcgcccgcaa gctcaccgag ccgatccaga
     6061 ccctcgtcgc gggcacgcgt gcagtggcga agggtgattt cgacacgcgc ctgcccatgc
     6121 cggcacgcga cgaaatgggc ttcctggtgc acagcttcaa cgacatgatg acgcggctcg
     6181 ccggcgcccg cgaactggcg cgccagagcc agcaggcagt ggagagccag cgcgcttacc
     6241 tggcggtcat cctcgggcgt ctctccaccg gcgtggtgtc gctggagcgt gactggcgca
     6301 tcaagaccgc gaacgaggct gccggcgcga tcctcggcgt ggacctggag ggcgcgattg
     6361 gccgtcccct ggcggaggtg gccgaggacc ggccgctgct gcagcagttc atcggggtcg
     6421 gccgcgagca cgtcgaggcg ggcgaattcg aatggcgcga gcagatcgtg ctgcgcaacg
     6481 aggcgggccg gcgggtgctc atgtgcgcct gctccggcct gcccggcgag gacggcgaac
     6541 cggacgggtt cgtggtcgtg ttcgacgaca tcacggcgct gcttcaggcg cagcgcgacg
     6601 ccgcgtgggg cgaagtggcg cgtcgcctgg cgcacgagat caagaacccg ctgacgccga
     6661 tccagctgtc cgccgagcgc ctgcgacgcc gcttcctcga gcagatgagc gacgatgacg
     6721 cgatggtgct cgagcgcgcc acccgcacca tcgtccagca ggtcgaggcc atgaaggaaa
     6781 tggtcaacgc gttctccgat tatgcacggg ccccggacat ggagatcgcg cgcgtggacc
     6841 tgaaccagct cgtcgcggag gtggcggacc tgtaccgggt gagggaaata ccggtcacgg
     6901 tccagctgag cctcgatccc ggcgtgacgg caatcgaggc cgacagcggc cgcgtcaggc
     6961 agatcctcca caacctgatc cgcaatgccg tcgaggcgct cgagggccat gacgacgccg
     7021 tcgtgcggat cacgacccgc ggcaccgacg agttcgatct tcgggcggtg ttcatcatgg
     7081 tcgaggacaa cggccccggc attccgcccg actcggtgga ccggatattc gatccctacg
     7141 tcaccagcaa gccgaagggc acgggtctcg gcctggccat cgtgaaaaag ctggtcgagg
     7201 agcacggcgg atgggtcgag gccagcaacc gcgccgaggg cggcgtgcgg gtcaccatcg
     7261 ccttgccggc ggatgaggat gcgcgcagcg cgatgctgcg gcacgaggtc cgcgcaagtg
     7321 aactcaggag agcgcgcgca tgagtaccgg caggatcctg gtggtggatg acgaggtcga
     7381 tatccggcag ctcctcgacg agattctctc ggaggagggc tacgaggtga gctcggccgc
     7441 gaatgcggca caggcgcggc gccaacgcga ggccgtcagg ccggacctgg tgctgctcga
     7501 catctggatg ccggatgccg acggcatcag cctgctgcgc gagtggtcga ccagtgagga
     7561 tctccgcccc tgcccggtgg tgatgatgtc cggccacggc acggtggaaa ccgccgtgga
     7621 ggcgactcgc cttggcgcct tcgacttcat cgagaagccc gtgtcgctgg ccaagctgct
     7681 gcgcacggtc gagcgcgcgc tcgagtcggg ccgggcgccc gggcgcgccg cccgccagct
     7741 ggtgccggcc accgtcgtgc cggtcggccg cagccgtctc atgcaggcgc ttcgggagca
     7801 gagcgaacgc gtcgccgcgc attcgacccc ggtactggtc accggcgagc cgggcaccgg
     7861 gcgtgaatca ttcgcgcgct acatccactc gctggggcgg caggcgcgcg ggccgttcgt
     7921 acggatggtc gccagcgccc tgcaggacgc ggatgcggag gcggcgctgt tcggcagggt
     7981 ggtcgacggc ggcaccgaac ccgggttcct ggagcgtgcc cgcggcggga cgctgttcat
     8041 caacgagctg tcggacctgt gcccgatggc ccagcgcctg ttgctgggcg cgctggagaa
     8101 cgggcgcttc acgcccgtcg gtgacgccga gccgcgcgcc atggacctgc gcatcatcag
     8161 ctccgcgccg gtcgggttcg agcatgtgcc ggccaccgca gcatttcgcc gcgaactgct
     8221 gacccagctg gcggtggtgg tcctcaaggt gccgccgctg cgcgagtact cggaagacgt
     8281 cccggaactg ctgcgccact acatggacaa gctggtcgac accgacggcc tgccgtttcg
     8341 caagctcagc gtcgccgcac agaaccgcct gcgcaattat ccgtggcccg gcaacgtgcg
     8401 ggagctacgc aacctcgtcc atcgcctgtt gatcatgggc ggcgaagagg agatcggcct
     8461 cgaggaagtc gagcaggcgc tcggcgagca actgtcgccg gacgaagggc tgctcagcca
     8521 ggacctgatg gcgctgccgc tgcgcgaggc gcgcgagcag ttcgagcggg cctacctgca
     8581 gcagcagctg caactctgcg agggcaaggt cgggcagctg gccaagcgcg tcggcatgga
     8641 gcgcacccac ctgtaccgca agctgcgcgc gctgggcatc gatttccgca gcagcggcac
     8701 cgacgactga tgccagtgcg tcagtcccgc tcgcgcaccg ccagtgcgcc gatgttggcg
     8761 tccgccaggc gacggcgggt ggcctcggcc tcggccgcgc cagggagcgg gccgatgcgc
     8821 acccggtgcc aggtctgccc ctcgatcgtc accgcctgga tgcttgagcc cagcccgagc
     8881 agcgcgagct ccgccttgcg ccggtcggcg tcctggtagc ggcggaagga accggcctgg
     8941 atccagtaac ggcccggcgc cagcgcgggt gccggcgcgg cggggccggc gccgcgggga
     9001 cggacctcct cctccggtac cacgacctcg agcccgggca gcatggtgta gaaatcgaag
     9061 ccggaggccg gcgccggctc gatctccgtg gcctcgtccg cctgctgtga ggccggcttc
     9121 gcgacacgcg cggcgaccgg ctcgtccggc gccgttgacg acgtgtgccg caggtgtacc
     9181 gccagcgcca cggacagccc cagcgccagg ccggtcagga gccacgccca gcccggcgtc
     9241 gctggcttcc tgggccgccg ccgcttgtag tcgcgcggca tgcctacatc gactccggcg
     9301 cgctgacccc gaccagcgcc agcccgttgg cgatcaccac gcgggtcgcc tcggccagcg
     9361 ccaggcgtgc ggagcgcagc ggcgcctcat ccaccaggaa ggtctcggcg ttgtaccagg
     9421 tgtggaagtc ctgggcgagc tcgcggaggt agtgcacgat gttctgcggc gcgcggttcg
     9481 cggccgccag ctccacgact tcggggaagc ggccgaggct ggccatgagc acgcgttcct
     9541 ggtcggtctt cagcaggcgc aggtgcgtga cggcctcgtc cgccgaccac tccagcccct
     9601 tttccaccag ctggcggaac acgctgcgca cccgggcgtg cgcgtactgg atgtagtaga
     9661 ccggattctc gttgctgcgg gacttggcca gctccaggtc gaagtccagg tgctggtcgt
     9721 tggagcgcag cacgtagaac aggcgtgccg cgtcgttgcc gacttcctcg cgcagctcgc
     9781 gcagcgtgac gaactccccg gagcgcgtcg acatctgcac cttttcgccg ccccgataga
     9841 ggattgcgaa ctgcaccagc cgcacctcga ggctgtcgcc cggctcgccc atggcctcga
     9901 gcccggcgcg cacgcgggcc acgtaaccgt gatggtcggc gccgaggatg tcgagcaggt
     9961 ggtcgtggcc gcgctgccgc ttgccgaggt ggtaggcgat gtccgacgcg atgtaggtgg
    10021 tctggccgtt gtcgcgcacc acgacacggt ccttttcgtc gcccagcgcg ctggcacgaa
    10081 accacaacgc gccgtcgcgt tcataggcgt gcccgtcggc cttgaggcgg tcgatacact
    10141 cggccacgag tcccaggtcg gtgaggctgc gctccgagaa ccattcgtca taggtgacgc
    10201 cgaactccgc caggtcgtcg cggatatcgc cgagaatctc gtccaggccg agcttgtgca
    10261 ccgcgcggta gtcggcatcg ccgagcagct cgcgcatgcg cgccaccacg gcgtcgatgt
    10321 aatcgtcctt gtcgccttcc ggcgcgtccg gcggcaggcc ggtgaacacc tcggcggccg
    10381 ggcggcgcag gcgggcgtgc tccagcgcca ccagcttgtc ggcgatcggc tggacgtagc
    10441 cgccgcgata gccgttggca gggaacgcga attcctcgcc cagcgcctcg aggtagcgca
    10501 gccacacgct ggccgcgagg atgtccatct gccggccggc gtcgttgacg tagtactcgc
    10561 gatgcaccag gtggccggtg gcctccagca ggttcgcgac cgaggcgccg taggcggcgt
    10621 gccggccgtg cccgacgtgc agcgggcccg tggggttggc ggaaacgaat tccaccagca
    10681 cgcgctgtcc cgcgcccacg tccgatcggc cgtaggccgc acccgcggcg agcacgccgg
    10741 ccgtctcccg ctcgaaggcg cgtcgactca gccggaagtt gatgaagccc ggccccgcga
    10801 tggagacgct ctcgacgagc tccgaggccg gcagcaggcc gacgatccgg tcggccagct
    10861 cgcgcgggtt catgcccgcc ggcttggcca gcagcaacgc ggcgttggtg gcgaaatcgc
    10921 cgtgacgcgg gtcgcgggcg cgctccacgc gcgccgctgc gacgtcggtc agcgagagct
    10981 gttcgcagag cgtcgcgagc gcgtcggcca gcaggccttc gatctgtttc ttcaagtcgg
    11041 gaataccggg ttgggtgggc ggttggccgc tcattgtacc gttccgcgcc cgcgctgttc
    11101 agggccctgg ccctgccgtg ttcctagaag aggtcctgcg gatcgacctc gaaaaaccag
    11161 cgtacgctgc ggctctcggg caggtccggg gcgatggcct tgagctcggc cagggcggcg
    11221 tgcagggtgg gccgcgcgcc ggactgcagc agcagttgcg cccgccagcg gccactgcgt
    11281 ttttccatcg gcgccggcgc cgggccgagc acgacgagcg tcgcggacgg gtgtaggcgg
    11341 gcccggcagg cggcgagaaa acgcagcgcc gcgccgggcc gggccgcctc ggcgcgcagc
    11401 agtgcaaggt ggctgaaggg tggccagccg gcggcacgcc gttcctccag cgcggcttcg
    11461 gcgaacgcct cgtagccgcc gcccagcagg cgctgcaaca ggggatgctc cggatacgcg
    11521 gtctgcacca gcacttcgcc gggacggtcg gcgcgccccg cccggccgga gacctgcacc
    11581 agcgtctgcg cgaggcgttc gccggcgcgg aagtccgtgc cgaacaggcc ctggtcggcg
    11641 ttgatgacgc cgaccagcgt gacgccgggg aagtcgtggc ccttcgccat catttgcgtg
    11701 ccgacgagga tgcgcgtgcg gccgtcgcgg acgtcgtcga ggatccgctc caccgaaccg
    11761 cgtcgccgcg tggcgtcgcg gtcgatccgc gcgatcgtca cgcccgggaa atgcctcgcg
    11821 agggcgtcct cgacgcgctc cgtgccctcg ccggccggcc gcaggtccgc gccgcaggcc
    11881 gggcagccgg cgggcagcgc cgtatcgtcg ccgcaatggt ggcagcgcag gcggccgatc
    11941 cgccggtgca gggtgtagcg cgcgtcgcag tggcggcagt cggcggccca gccgcaggaa
    12001 ctgcagaaca gggccggcgc gtagccgcgc cggttgagaa agagcatggc ctggccaccc
    12061 gcgtcgaggt gcgcgcgcat cgcgccgagc agcggcgccg acaggccctc ggtggcggga
    12121 tggcggcgca ggtcgatgac cgagacgcgc ggatgcgtcg acgcgcccgg ccgctcgggg
    12181 agcctggaga gcgcgtaacg gccgtcgcgc gcgtgcttaa gggtttccag cgagggcgtc
    12241 gcggagccga gcacaatcgg gatgtccgcc aggctggcgc gcatcaccgc cacgtcgcgt
    12301 cccgagtagc gcatgccctc gtgctgccgc agcgatggat cgtgctcctc atcgacaatg
    12361 atcaggccgg ggcgcgccag cggcacgaac acggcggagc gcgtgccgat gaccacggcg
    12421 gcacggccgc tccgggcatc cgcccaggcc gtcatgcgct cgccgtccgg cagcccggaa
    12481 tgcagcaccg ccaccggcgc ggccagccgc gccgcgaagc gctgcaccag ctgtggcgtc
    12541 aggccgattt ccggcaccag caccagcacc tgcagaccgc gtcgcaggca ttccgcggcg
    12601 gcgaccaggt agacctcggt cttgccgctg ccggtcaccc cgaacagcag ccggggcgag
    12661 aaccgtccca ggtcgccgat gatggcggct gcggccgcag cctgggcagc gttgagcgtc
    12721 ggtccggcca gcgcggctgt ccaggcggcc gccgccggct cgggctcgat ttccaccagc
    12781 ccccgggccg ccaggcgcgc caccccggca cgccaccccg gcggcagcgc cggatcgtcg
    12841 gtgcggcgcg gcgcggccgc cagcaggtcc agcagggcgg cctgtttcgg tgcgcgtttc
    12901 ttcatcgcct ccggcgcggg accccgggcg acgacccggg cagggggctc gggcagcggc
    12961 ttgcccctgc gcagcggcgc cggcagggcc gtggcgacca ccgggcccgg cggctcatgg
    13021 tagtagcgcg ccgtccagtc gaggagcttc agcagtgccg tgtcgaacag tggtgcagcg
    13081 tccagggcct cgagcaccgg cttgaggcgt tcgtccggca ggtcggactc ggcggcgtgc
    13141 gccatcacca ggccgacggg gcgccgccgc ccgaagggca cgcgcacgcg cacgccgggc
    13201 gccggcggtg ggcctgaatc gggggcggga tagtcgaaca gggtatcgag cggggcgtcc
    13261 accgcgacct tgaggatggt catgagtggc ggacctgcga ccgggccgca agttgtccac
    13321 agaacctgtg gataactctg tggaagagtg gttgcaatcg cggtgcggtc cgcatcactt
    13381 caagggggcg ctcagtttgg tcaaaaaaac atcataaaaa taactatata taaaacagtc
    13441 tattgcgtgc tcttgttggt gtactttgtt ttgtgacaac gatttgaagc ggtattttcc
    13501 gggtcttgtg aataagtgac tccggcgatg acctttgcgg gcagcgccaa gcccttgtcc
    13561 gggctcgatt cagccacccc gcggcggggc gccaacgctc cgttgagcgg ccgcttcggc
    13621 cactttgtta aggggtccgg gccgccgccg gtgcggccga ggcctcagcc ggccttgatg
    13681 gcgtggatca gctcggagac cgccgcctgg ccgtccccgt acagcatccg ggtgttgtcc
    13741 aggtagaaca gccggttctc gatgcccgag aaacccttgc ccttgccgcg cttgatgacg
    13801 atgacgttct tcgccttgtc ggcgttgagg atcggcatgc cgtagatcgg gctggaaggg
    13861 tcggtgcgcg ccaccgggtt gaccacgtcg ttggcgccga tcaccagcgc cacgtcggtc
    13921 ttctcgaagt cggcgttgat ctcgtccagg tcgaagatca ggtcgtaggg cacgcccgcc
    13981 tcggccagca gcacgttcat gtggcccggc atgcggcccg ccaccgggtg gatggcgaaa
    14041 ctgaccttga cgccccgctc ccgcagcagc tgggccatct cccagacctt gtgctgcgcc
    14101 tgcgccacgg ccatgccgta gcccgggatg atcaccacgc ggtcggcgaa ggccatcatg
    14161 atgccggcgt cggaggcctc gatgggcttc atgctgccgc tgacctcgcc gctgtccacg
    14221 gactcgtcgc cgaaggcgct gaacagcacg ctggccagcg agcggttcat ggccttggcc
    14281 atcagctgcg tcagcaaggt gcctgctgcg ccgacgacgg tgccggcgat gatcatcgcc
    14341 tcgttgtcga gcacgaagcc cttgaaggcg acggccaggc cggtcagcgc gttgtacagc
    14401 gagatcacca ccggcatgtc ggccccgccg atcgggctgg tcagcatgat gccggccacc
    14461 agggccagca cgaagaacag cgtcaccagg gtgccgtggg ccgagtaatt gcccagcagg
    14521 aacatgccga tgatgaaagc gctcagcagc aacgccgcat tgagctggtt gatgctcttg
    14581 aagcgtatcg gcttcgtcat ccagccttgc agcttggcga aggcgatcag gctgccggag
    14641 aaggacaccg agccgatcag gccgccgagc acggcgagga agccgaccgt gagcgaatcc
    14701 ggcgcgccct tgtacagggt gaccgccgcg atcgcgccgg cggcgccgcc gcccatgccg
    14761 ttgtacaggg cgatcatctg cggcatgtcg gtcatcgcga ccttcttgcc ggtccaccag
    14821 gccaggccgc cgccgagcag gattgcgatg atgatcagcc cgatgttgac ggcgctcatg
    14881 ccgggccaga agaaggtcac cacggtcgcc aggaccatgc cccagcccgc ccagagaatg
    14941 ccgccgcgcg ccgtgcgcgg cgagctcatg cgcttgatgc cgatgatgaa cagcagggcg
    15001 gcgataaagt agctcgcctt gatcatgaag gtcgcgaagc ccattacttg cgctccccgc
    15061 tggacttgaa catctcgagc atgcgctccg tgaccacgta gccgccgacg gcgttgcccg
    15121 cgccgagcac cacgccgatg aagccgatga cctgcccgag ggtgctgtcg gccttgccga
    15181 gcgcgaccat gcagcccacc agcacgatgc cgtgcacgaa gttggagccg gacatcagcg
    15241 gcgtgtgcag gattaccgga acccgcgcga tgatctcgta gccggtgaag gcggcgagca
    15301 tgaaaatgta cagcgcgatg aaaaaccctt cgattccgcc gatcatgtcg actgtcctcc
    15361 gctggctccc tcgatgcgct cgcgggtggg tgcgtggccg atccggccgt cgtgcgtcag
    15421 cacgctgccg gcgaacacct cgtcctccca gtccaccgcc agcgtgcctt cgctgatggc
    15481 gggcgacagg aagttgaaga ggttcttggc gtacatctcg ctggaatgcc gtcccacggc
    15541 cgccggcagg ttgaccgggc cgaccacctg cacgccgctc gcatcgacga cttcgccggc
    15601 cttggtcagg gcgcagttac cgccagtctc cgcggccagg tcgacgatta ccgagccggg
    15661 ctgcatgcgc ccgaccgtct cggcgtcgat gatgcgcggg gccgtgcgcc ccgggatcgc
    15721 cgcggtcgtg atcacgacgt ccgccgcagc cacgtggtcg gcgaggatgc ccgcctgctt
    15781 ggccttctcc tcgtcggtca gctcgcgcgc gtagccgccc gtgccctccg cgctgatgcc
    15841 ggtgtcgacg aacttggcgc ccagcgactg gacctgttca cgggtcgccg agcgcacgtc
    15901 gtagccctcg acgatggcgc cgaggcgctt ggcggtggcg atggcctgca gccccgcgac
    15961 gcccgcgccg atcaccagca ccttggccgg gcgaatggtg ccggcggccg tggtgagcat
    16021 ggggaagaac ttgtcgaggt gcatcgcgcc gatcagcacg gcgcggtagc cggccaccgc
    16081 cgcctgggac gacagcgcat ccatggattg cgcacgcgag atgcgcggca ccagctccat
    16141 ggagaagctg gtgatcctgc gatcacgcag caggcctacc agctcgtcgg aacgatgcgg
    16201 ctggagcagg ccgatcagca cggcgttctc gcgcagcccg gcggccacct cgagcggcgg
    16261 ggggttgacc gcgatcagta cgtccgcccg gctgaccact tcctcgcggc cgacgaactc
    16321 gacgccctcg aaacggctgt ccggtatacg tgcggcctcg gcaatgcggt gctcggcgca
    16381 aatcgttgcg cccagggcgg cgaatttctt cgcgacctcg ggaaccagcg gcgaacgcgt
    16441 ttcccctgcg gcggtttcgc gcagggcgcc gatgaccaat gccatgggca acctccccgg
    16501 agcctgactc ggcccttgtt cttacggcgg atgagtgtac aggaagatga ttggcagttg
    16561 tctgcctgat tgattttcgc cagcccttct ggaataacac gggcagcggc ggagccgcgc
    16621 aaacggaacc gagttcgcga tgggtatgtc cctgagccaa cagatcctgc gcaccgacgt
    16681 cgccggcatg ccgcttgagt gggtgga
//
LOCUS       DAOMPG010000024        16427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1537_length_16427_cov_3.630894, whole genome shotgun sequence.
ACCESSION   DAOMPG010000024 DAOMPG010000000
VERSION     DAOMPG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16427
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_16427_cov_3.630894"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLS34_02280"
     CDS             <1..757
                     /locus_tag="PLS34_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRP86334.1"
                     /translation="QWFDIALIAAVLLSVSVVMMATVESLDAKYGRLFYAVEWFFTVL
                     FTIEYGLRLYCVKHPKRYARSFFGVVDLLSVLPTYLGLILTGTAPMLVIRVLRILRLF
                     KIMHMDRYVGEANILLEALVASWRKTLVFVYAVLTLVVIFGTLMYFIEGAPAGFTSIP
                     QGMYWAVVTLTTVGYGDVTPLTTLGKVIASLIMITGYGIIAVPMGIYFSEFTQASKRR
                     GRTRSCPSCKLKGHETDAVHCRACGAPLDGEKA"
     gene            801..1790
                     /locus_tag="PLS34_02285"
     CDS             801..1790
                     /locus_tag="PLS34_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86335.1"
                     /translation="MPRRIVVGLNAVIFAVRDDEARVLTVDMPTPAGGAEPGDDGAAA
                     RERVALPSGPFDPARDRTLELGLRAWVREQTGRDLGYVEQLYTFGDEGRDPREHAGGP
                     RMLSIAYLALTQDLAAAHTGGQWISCYRFLPWEDWRTGRPAVLDQIAASLDGWAARAR
                     SAAERERRAGRIEICFGLGGAGWDGYRVLERYELLYEAQLTHESWLDRGRRPPATARL
                     FGKPMAHDHRRILATGLARLRGKLRYRPLVFELIDEHFTLARLQHVVEALSGLRLHKQ
                     NFRRLVESGGLVEKTGRIETRTGGRPAELFRFRREVLRERRAPGVGALSPRRT"
     gene            1909..2907
                     /gene="nadA"
                     /locus_tag="PLS34_02290"
     CDS             1909..2907
                     /gene="nadA"
                     /locus_tag="PLS34_02290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRP86336.1"
                     /translation="METELRYTPEVARQTRPLYEGLRHVIPEVEWGVHAPYIAAINKL
                     KREMNAVLLVHNYQTPEIFHGVADYTGDSLGLARQAAKTDADIIVQCGVHFMAETSKI
                     LSPDKTVLIPDLEAGCSLAASITGGDVRLLKERYPGVPVVTYVNTSADVKAESDICCT
                     SGNAVRIVESLGADRVIFLPDNYLGRYVAGQTGVELILWHGTCEVHERFTGDELREYR
                     QRFRDIQVIAHPECPPDVLAEADFVGSTKGMIDWVGEHRPARVVMITECSMSDNVAVE
                     HPEVEFIRPCNLCPHMKRITLPKILRSLQTRTHEVRVDPQTAARARRAVEAMLEVA"
     gene            2904..4532
                     /gene="nadB"
                     /locus_tag="PLS34_02295"
     CDS             2904..4532
                     /gene="nadB"
                     /locus_tag="PLS34_02295"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRP86337.1"
                     /translation="MNAAGDILVIGAGIAGASAALHLADAGRHVTVLARTPLNASASA
                     QALGGIAAAIGPDDAPALHLEDTLAVGGGLCREATVRGVVERAPAAIDWLLAQGVEFD
                     RDGDHLDLGQEGAHRRRRVVHVADATGRAIMAVLGRRLEAHPRITLLAQHAAVELLVR
                     SEPAVPGGRACIGARVLDQRHGLLHTLHADHIVLATGGASGVYSVSTNASRPVGDGIA
                     LAWQAGCRVADLEMVQFHPTALRHPAAEGWLVTEAVRGEGGRLLLPDGERFMFRHDPR
                     GELAPRDIVARAIHAEMQAHKLEYVLLDISHADAGFIRHHFPNTWAKCLELGIDITRE
                     PMPVAPAAHYTCGGIVTGGAGQTDLAGLYAIGETAWTGLHGANRLASNSLLEGLVYAE
                     AASAAILADVPRGPVLSPAGALGALQPVPATPAPQALGEARQLARAIRRLMSREVGIV
                     RSDHGLASAVAQLGRLQARCDVLYARHGPSATLLELRNLAAVAGLVASSARLRHESRG
                     AHYNRDHPEPGPRALSTVLQPAHDALLLDPRAVA"
     gene            4605..5102
                     /locus_tag="PLS34_02300"
     CDS             4605..5102
                     /locus_tag="PLS34_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRP86338.1"
                     /translation="MGLEDKLARTERKEEQAKRLAERIGDKLVETFQVAALFIIGAAI
                     FYSAAVFLLELVAAREVSLHDILLLFIYLELGAMVGIYFRTDELPVEFLLYITITVMT
                     RKLISAEDLSESRIMVTTGAVLILAVAVLLLRFGAHRFPSGGPGRDKQAAEIDSAPTP
                     KGTGA"
     gene            complement(5077..5667)
                     /locus_tag="PLS34_02305"
     CDS             complement(5077..5667)
                     /locus_tag="PLS34_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protein"
                     /protein_id="HRP86339.1"
                     /translation="MASIPEWFKILYALAVLAFMVVYWLRYGPRNFLWFSDIALIGAV
                     PAMWLESPLISGTLACMVLLPEVLWNVDYVLRIVLKRRITGLTEYMFDASIPRWLRAI
                     SLFHVPLPLVMIWLVVAYGYPSASLPVAAGTAAVVLALSYLLSSREKNINWVFGLGRV
                     QHRLPQPLYLALLYLGFVLVVFLPTHWLLMRLFPSA"
     gene            5712..7805
                     /locus_tag="PLS34_02310"
     CDS             5712..7805
                     /locus_tag="PLS34_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86340.1"
                     /translation="MPRPVRRGAMFRVLRLAGLLASLHCGPAAGDAALQFGIAALDGP
                     GWRATDVRVALHRADSGRLAATLSAARLELPAPVGALQDVSAECLDLAVSAARFECRS
                     LRLAPGATAPEPLRKLAGRVAFRRDTGAVSWALAPADTATLEFSGDLEADRWRVRLDG
                     REWPLASLASFAALLGRPLPVLGGSADFSITARGAGEQLEGAVFELRAAGVGGANDAG
                     TMAAEDLRLELKGSAWRVDDALAFDARGTITAGEAYVEPVYTDLSTHPVRLNLRGRLH
                     GERLEISRLVAVQDDVLHLDARGELLRTAADGWRPGGGRLRLAEAALPGAYVTFLQPF
                     LAGTPLGDLETSGLLRGEVEYTADGIAAVWLELAGVNLDDRTARLAVYELNGELAWAP
                     ARDGGMTARARPADLRWSGGFVYGIPFGAARLRMDSWGGRWSLPFPVSIPVLDGALEI
                     EQLEFGDFMAGDDDIRFEARLAPISMRELSRALDWPPLSGRLSGALPRLSYQDGVLSL
                     GGDLSAEMFSGSVAVRNLRIERPLQPLARLQADIEIRGVELAELTQALSFGLMTGRLD
                     GYVRGLEMIDWAPVAFDARLYTPPDSRARQRISQRAVDNIASIGGGGAGVLSTGFLRF
                     FEDFSYAGFALGCRLERDVCRMSGLETRADGYVILRGSGLPRIDVMGFARDVSWSALV
                     AQIAGIMAAEDAEIR"
     gene            7883..8506
                     /locus_tag="PLS34_02315"
     CDS             7883..8506
                     /locus_tag="PLS34_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HRP86341.1"
                     /translation="MRRLSLKLLLATTLFAAACVTINVYFPAAQAERAADRIITDVWG
                     TEPAKRDNEGEPQGRLDPQRWVPDAVDQVRLAALSALDFIVPRAEAQADFDIETPEIR
                     ALTASMNARHRQLKPSYDSGAIGLTADGLVDIRDRNLVPLAERNAIRQLVDAENRDRN
                     ALYKAIAQANGKPEWEADIRKTFAQRWIGRAEPGWWFRTAGGQWEQK"
     gene            8541..8876
                     /locus_tag="PLS34_02320"
     CDS             8541..8876
                     /locus_tag="PLS34_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86342.1"
                     /translation="MNDFVRMLAAWALVHESADEGWKAAVARGRKAEPGDLEQGPEAF
                     VDGLAALVAEEKDRLKAHLAARDDATTPARLDLDARLDAIAFELAELRGRLESLQASI
                     DAIAARGRD"
     gene            8883..10241
                     /locus_tag="PLS34_02325"
     CDS             8883..10241
                     /locus_tag="PLS34_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRP86343.1"
                     /translation="MSRRGRAIAAVAVRRLAAASWRARFGLRRGHRRREVVAREARLA
                     CEALGPAFIKLGQLASVRPDVFSPETVFELERLQDRIPAVPVDEIRAIIAAELGAPPE
                     ALFRSFDPDPIATASIAQVHSAVLACDYRPVTGAVLPAGTRLALKVVRPGVERVIAAD
                     LAVARRWADRAERLGRFARLRPRALLAEFENTLASELDLRNEGRTADRFARDFATDPL
                     VVVPRVVWRHTTRRVLATEFVNGWRLNDLGEAERSGIDARRLAAHGAEVFMRQVLVHG
                     RYHADLHPANLFVTPDGRICYLDFGITGRTTPAQRIAIAQVLAATVYGDAERALRYSR
                     ELGLAIPDHREPAVRAAVAALMKRHLAGPGRADVRGFALGFLSMLADFRIAIPEGYGL
                     LVKALVTVEGVARAIYPDIDIIEAAGPFATRLIAAQLARPERLRERVPAALKAATREL
                     LA"
     gene            10264..11325
                     /locus_tag="PLS34_02330"
     CDS             10264..11325
                     /locus_tag="PLS34_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRP86344.1"
                     /translation="MKFLAIALLVLLAWLAFWPVPIEPVAWDAPAAPALEGRYALNDR
                     LAGVTRHGRGIGVGPEDVAVDAAGNVYVGYEDGRVMHFPAGDAAPQLLADTGGRPLGM
                     ELDADGRLVVCDGHRGLLRIAMDGAVEVLATAADGLPFGFTNDLDIAADGRIYFTDAS
                     WKFGPAMKARDDILEHGGHGRLLEHDPATGRTRVLLDGLQFANGVALAPDGHAVLVAQ
                     TGRYDVLRYWLHGPLAGTHEVFIDNLPGIPDGLSSNGRDTYWLALFTVRNPALDALSD
                     WPWLRKVVYRLPQFLQPQPARHAFVLGLSLDGEVVHNLQHAAPDAFAPVTSATEAHGR
                     LYLGSLTQDAFAVLDLAGP"
     gene            complement(11339..13498)
                     /locus_tag="PLS34_02335"
     CDS             complement(11339..13498)
                     /locus_tag="PLS34_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86345.1"
                     /translation="MTDKNKAGGAHAWRFFRYGGFDQVAIETGEDLRHLAELDPKLWT
                     ALSCPTTGLEFDARTLALLDTDGDGQIRVPEILGAVRWACERVRDPQVLFEDGELPVE
                     AIDDSHAEGAVLKAAATQVLDYLGKSEAGRLGVADFADMTRLFTPDHFNGDGVLPAAL
                     AGDEALAQVVGEIVATQGGVPDRSGEPGITAETLEAFFAQVDEVLAWRHRAAESAALI
                     LPLGEATAAAAGAFEAVQAKVDDYFTRCRLAAFDARAGEALGAPVTVYGALAERVIDT
                     GDADVAALPLAQVAAGSPLPLGKGVNPAWAGRIAQLHDEVIEPLLGTRETLTAQEWAE
                     LSAKFTAYRAWMADRPATQVHELELGRLEQLKSGDTRARLAALIDKDLKAETSAGNID
                     AVERLVRYQRDLVTLLRNFVTLSDFYGGADKAVFQAGTLYLDQRSCELVMRVSDMPKH
                     ATLAPFSGCYLVYCTCVRAGSGPIDIVAALTGGEVDELMVPGRNGMFVDREGRDWKAT
                     VVKVVAQPVSIRQAFWTPYRRLGALIENQIRKFAASRDKDVENKTAANVEGAATKVEG
                     GQPAAAPPFDIAKFAGIFAAIGLAVGAIGTALAAAVAGLFKLAWWQLPLVVVALALII
                     SGPSMLLAYMTLRRRNLGPLLDANGWAVNARARINVPFGGSLTAVAELPKGASRSLQD
                     PFAEKKRPWKTWLFLLALAVVLLVLYRQGMFAGLLGG"
     gene            complement(13504..14019)
                     /locus_tag="PLS34_02340"
     CDS             complement(13504..14019)
                     /locus_tag="PLS34_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRP86346.1"
                     /translation="MGAGLARLAERQHLFVALLSGWLILTSPWVHMFRRIPGNAGWLD
                     YAHVVLGLAAAVLAVTFTASCTRHGRWRLYFPWLTGALAQPARELRGLLRGEVPSAEG
                     GGLFALVEGLLLLALLATALSGCAWLLVQGSAAAVDWRGYHILAARGMMALLVLHVVA
                     ASLHLLDFIRE"
     gene            complement(14027..14569)
                     /gene="hemG"
                     /locus_tag="PLS34_02345"
     CDS             complement(14027..14569)
                     /gene="hemG"
                     /locus_tag="PLS34_02345"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011864484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HRP86347.1"
                     /translation="MANILLLYSSVYGHTRKICDRIQARLVELGHQVEAVPVADGALD
                     PARYDAILVGASIRNGKHNPAVLDFIARHKDLLDARPSAFFSVNLVARKPAKNTPETN
                     PYTKAFVAKSPWQPKLLGVFGGDLDYQRYTAFDRNVIRFIMWLTKGPTDPATKDEYTD
                     WGEVDRFATRFAALLDGRPG"
     gene            complement(14653..15786)
                     /locus_tag="PLS34_02350"
     CDS             complement(14653..15786)
                     /locus_tag="PLS34_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRP86348.1"
                     /translation="MTFHSFHPPVRTLMGPGPSDVHPRVLEAMGRPTIGHLDPQFVAM
                     MDELKQLLQYAFRTTNPLTIPVSGPGSAGMEAAFVNLVEPGDTVIVCINGVFGGRMKE
                     NVERCGATAVVVEDPWGHPVDPDKVALALKEHPEASILAFVHAETSTGARSDAETLCR
                     LAHEHGCLVIMDAVTSLGGIPVEVDAWGVDAVYSGSQKCLSCTPGLSPVSFSPRAVER
                     IRGRTHKVQSWFLDLNLVMGYWGSGDATPKRAYHHTAPINALYGLHESLVMLAEEGLE
                     ASWARHRAMHEALGAGLEAMGLEFIVDAPWRLPQLNAVRVPVGVDEAAARQRLLEEFN
                     LEIGGGLGALAGKAWRIGLMGYSARPANVKLCLEALGEVLGAR"
     gene            complement(15812..>16427)
                     /locus_tag="PLS34_02355"
     CDS             complement(15812..>16427)
                     /locus_tag="PLS34_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86349.1"
                     /translation="DERQVAGAVEAAVERFGGIDVVVNNASAINLTPTEATPIRRFDL
                     MMGVNMRGTFLVTQACLPHLKQSAAAGRNPHVLTLSPPLNMDAKWFAPHVAYTMAKYG
                     MSMCVLGHSAEFKPFGIAVNALWPRTVIDTAALQMIPGVKPEHCRTPAIMADAAHAVL
                     TADAAATTGNFFIDDEVLRAVGVTDLDKYSVVPGSKALLPDLFL"
BASE COUNT         2418 a         5893 c         5689 g         2427 t
ORIGIN      
        1 ccagtggttc gacatcgcgc tgatcgccgc cgtgctgctc agtgtcagcg tggtgatgat
       61 ggccacggtc gagagcctcg acgcaaagta cggccggctg ttctacgcgg tggaatggtt
      121 cttcaccgtg ttgttcacca tcgagtacgg cctgcggctg tattgcgtga aacacccgaa
      181 gcgctacgcc aggagcttct tcggcgtcgt ggacctgctg tccgtgctgc cgacgtacct
      241 cgggctgatc ctcaccggca ccgcgccgat gctggtgatc cgtgtgctgc gcatcctgcg
      301 gctgttcaag atcatgcaca tggaccgcta cgtcggcgaa gcgaatatcc tgctcgaggc
      361 gctggtcgcg agctggcgca agacgctggt attcgtctat gcggtgctga cgctggtggt
      421 gatcttcggc acgctcatgt atttcatcga gggcgcgccc gccggcttca ccagcatccc
      481 gcaaggcatg tactgggccg tcgtcacgct caccacggtc ggctacggcg acgtcacgcc
      541 gctgaccacg ctcggcaagg tgatcgcgtc gctgatcatg atcaccggct acggcatcat
      601 cgcggtgccc atgggcatct atttctccga gttcacccag gcgagcaagc gccgcggcag
      661 aacccgcagc tgtccgtcct gcaagctcaa ggggcacgag acggacgcgg tccattgccg
      721 cgcctgcggc gcgccgctcg acggggagaa ggcctgatgt gccaggctgc gcccggtgta
      781 ccggccggag gggccttgcc atgccgcggc gcatagtcgt cggcctcaac gccgtgatct
      841 tcgccgtgcg cgacgacgaa gcgcgcgtgc tgacggtcga catgccgacg ccggccggtg
      901 gcgcggagcc cggcgacgac ggtgccgcgg cgcgcgaacg ggtcgccctg ccctccgggc
      961 cattcgatcc cgcccgcgac cggacgctgg agctcggcct gcgcgcctgg gtgcgcgagc
     1021 agaccggccg tgatctcggc tacgtcgagc agctgtatac cttcggcgac gagggccgcg
     1081 atccgcgcga gcacgccggc gggccgcgca tgctgtccat cgcctatctc gcgctgaccc
     1141 aggacctggc cgccgcccat accggcgggc aatggatcag ctgctatcgc ttcctgccct
     1201 gggaggactg gcggaccggc cgcccggcgg tgctggacca gattgccgcc tcgctggacg
     1261 gctgggcggc cagggcccgc tcggcggcag agcgggagcg gcgcgccggg cgcatcgaga
     1321 tctgtttcgg gctgggcggc gccggctggg acggctaccg ggtgctggaa cgctacgagt
     1381 tgctgtacga ggcacagctg acgcatgagt cctggctgga ccgcggccgc cggccacccg
     1441 ccaccgcccg attgttcggc aagcccatgg cacatgacca ccggcgcatc ctggccaccg
     1501 gcctggcgcg cctgcgcggc aagctgcgtt accggccgct ggtgttcgag ctcatcgacg
     1561 agcacttcac gctcgcccgc ctgcagcacg tggtcgaggc gctgtccggc ctgcggctgc
     1621 acaagcagaa tttccgccgg ctggtggaga gcggcggcct ggtggagaag accggccgca
     1681 tcgagacccg taccggcggc cggcccgccg aactgttccg cttccgccgc gaggtcctgc
     1741 gcgaacgccg cgcccccggc gtcggcgccc tctccccccg ccggacctga aatggggaca
     1801 ttccccaatt cagctggaca agcgatatgc tcaggcgtag catatttcgc aggccggacc
     1861 gatggccttt ttttagccgg tactattgct cacattgagt ataagtagat ggaaacagag
     1921 ttgcgttaca cgcccgaggt cgcccgccag acgcgaccgc tctatgaagg cctccggcac
     1981 gtcatcccgg aagtcgagtg gggcgtgcac gcgccgtaca tagcggccat caacaagctc
     2041 aagcgcgaga tgaacgccgt gctgctggtg cacaactacc agacaccaga gatcttccac
     2101 ggcgtcgccg actacaccgg tgattcgctc gggctcgccc gccaggcggc gaagaccgac
     2161 gccgacatca tcgtccagtg cggcgtgcac ttcatggccg agacctcgaa gatcctcagc
     2221 cccgacaaga ccgtgctgat ccccgacctc gaggccggct gctcgctcgc ggcctcgatc
     2281 accggcgggg acgtgcgcct gctgaaggag cgctaccccg gcgtgccggt cgtcacttac
     2341 gtcaacacca gcgccgacgt gaaggccgag tcggacatct gttgcacctc gggcaacgcg
     2401 gtgcgcatcg tcgaatccct cggcgccgac cgggtgatct tcctgccgga caactacctc
     2461 ggccgctacg tcgccggcca gacgggagtc gagctgattc tctggcacgg cacctgcgaa
     2521 gtacatgagc gcttcaccgg cgacgagctg cgcgagtacc ggcagcgctt ccgggatatc
     2581 caggtgatcg cacatcccga gtgcccgccc gacgtgctcg ccgaggccga tttcgtcggt
     2641 tccaccaagg gcatgatcga ctgggtcggc gaacatcgcc cggcccgcgt ggtgatgatc
     2701 accgagtgct cgatgagcga caacgtcgcg gtcgagcatc cggaggtcga gtttatccgg
     2761 ccgtgcaacc tgtgcccgca catgaagcgc atcaccctgc cgaagatcct gcgctcgttg
     2821 cagacccgca cccacgaagt gcgcgtcgat ccgcagaccg cggcgcgcgc acgccgcgcc
     2881 gtggaggcga tgctcgaggt cgcatgaacg cggccggcga catcctcgtc atcggcgcag
     2941 gtatcgccgg cgcgagcgcg gcgctgcacc tggccgacgc gggccgccac gtcaccgtgc
     3001 tggcgcgcac gccgctcaac gcctccgcga gcgcccaggc gctgggcggc atcgccgccg
     3061 ccatcggccc ggacgacgcc cctgccctgc acctcgagga cacgctggcg gtaggcggcg
     3121 ggctgtgccg cgaagccacc gtgcgcggcg tcgtggagcg cgcgccggct gccatcgact
     3181 ggctgctggc gcaaggcgtg gaattcgacc gtgacggcga tcatctcgac ctcggccagg
     3241 aaggggccca tcggcgccgc cgcgtcgtgc atgtcgccga cgccaccggc cgcgccatca
     3301 tggccgtgct cggccgccgg ctggaagcgc acccgcgcat cacgctgctg gcgcagcatg
     3361 ccgccgtcga attgctcgtc cgcagcgagc ccgccgtccc cggcggtcgc gcctgcatcg
     3421 gcgcccgcgt gctcgaccag cggcacggcc tgctgcacac gctgcatgcc gaccatatcg
     3481 tgctggcgac cggcggcgca tcgggcgtct actcggtctc caccaatgct tcgcgcccgg
     3541 tcggcgacgg catcgcgctg gcgtggcagg ccggctgccg cgtggcggac ctggaaatgg
     3601 tgcagttcca cccgacggcg ctgcgccacc cggccgccga aggctggctg gtgaccgagg
     3661 cggtgcgcgg cgaaggcggc cgtctgctgc tgcccgacgg tgagcgcttc atgttccgcc
     3721 atgacccgcg cggcgaactc gccccgcgcg atatcgtggc gcgcgccatc catgccgaga
     3781 tgcaggcgca caagctcgaa tacgtgttgc tcgacatcag ccacgccgac gccgggttca
     3841 tcaggcatca cttccccaac acctgggcga agtgtctcga actcggcatc gacatcaccc
     3901 gcgagccgat gccggtcgct ccggccgcac actacacttg cggcggcatc gtcaccggcg
     3961 gcgccggcca gaccgacctg gcgggcctct acgcgatcgg cgaaacggcc tggaccgggc
     4021 tgcacggcgc caaccggctc gccagcaatt cgctgctcga ggggctggtc tatgccgaag
     4081 cggccagcgc cgccatcctg gccgacgtgc cgcggggccc ggtgctctcg ccggccgggg
     4141 cgctcggagc gctccagccg gtaccggcca ccccggcgcc gcaggcactc ggcgaggccc
     4201 gccagctggc gcgcgccatt cgtcgcctga tgtcccgcga ggtcggcatc gtgcgcagcg
     4261 atcacgggct ggcatcggcc gtcgcccaac tcggcaggct gcaggcacgc tgcgacgtgc
     4321 tgtacgcgcg ccacgggccg tcggccacgc tgctcgagct gcgcaacctc gcagcggtgg
     4381 ccgggctggt cgcgagcagc gcccggcttc gccacgagag ccgcggcgcg cactacaatc
     4441 gcgaccaccc ggagcccgga ccgcgcgccc tgtcgaccgt cctgcagccc gcccacgacg
     4501 ccctgctgct cgatccgcgc gccgtcgcct gaacccgttc aacggcacca cgaggccgcg
     4561 ttttgcgcta gtctcccccg catcggtcat cgggaggcgt cgacatggga ctcgaagaca
     4621 agctggcgcg gacagaacgg aaagaagagc aggccaagcg acttgcggaa cgcatcggcg
     4681 acaagctggt ggagaccttc caggtcgccg cgctgttcat catcggcgcc gcgatattct
     4741 attcggccgc cgtgttcctg ctggagctgg tggccgcgcg cgaggtgtcg ctgcatgaca
     4801 tcctgctgct gttcatctat ctcgaactcg gcgccatggt gggcatctac ttccgcaccg
     4861 acgagctgcc ggtggaattc ctgctctaca tcaccatcac ggtcatgacg cgaaagctca
     4921 tcagcgccga ggacctgagc gaatcgcgca tcatggtgac caccggcgcg gtgctgatcc
     4981 tcgccgttgc cgtgctgctg ctgcgctttg gcgcgcatcg attcccgtcg ggcgggccgg
     5041 gccgcgacaa gcaggcggcc gaaatcgaca gcgcgcctac gccgaaggga acaggcgcat
     5101 gaggagccag tgggtgggca ggaacaccac cagcacgaag ccgaggtaca gcagcgccag
     5161 gtaaagcggc tgcggcagcc ggtgctgcac ccggccgagg ccgaataccc agttgatgtt
     5221 cttctcgcgg ctgctgagca ggtagctgag cgcaagcacc acggccgcgg tgccggccgc
     5281 caccggcaac gaggcggacg gataaccata ggcgacgacc agccagatca tcaccagcgg
     5341 cagcggcacg tggaacagcg aaatggcgcg cagccagcgg gggatcgatg cgtcgaacat
     5401 gtactcggtc agcccggtga tgcgccgctt gagcacgatc cgcagcacgt aatccacatt
     5461 ccagagcacc tcgggcagca gcaccatgca ggccagggtg cccgagatca acgggctttc
     5521 cagccacatg gccggcaccg cgccgatcag cgcgatatcc gagaaccaca ggaagttgcg
     5581 cggcccgtag cgcagccagt agacgaccat gaaggccagc acggccagcg catagaggat
     5641 cttgaaccat tcgggaatcg aggccatgcg tcgggactat actggattgc aaacctgcac
     5701 tggagccgtc attgccccgc cccgtccgcc gtggcgccat gttccgtgtg ctgcggctcg
     5761 ccgggctgct cgccagcctg cattgcggac cggcggccgg cgacgccgcg ctgcaatttg
     5821 gcattgcggc actggacggc ccgggctggc gcgcgaccga cgtccgcgtc gcactgcatc
     5881 gtgccgactc cggcaggctg gccgcgacgc tcagcgccgc gcgcctcgag ctgcccgcac
     5941 ccgtcggcgc cctgcaggac gtcagtgccg aatgcctcga cctggccgtc agcgcggcgc
     6001 gtttcgagtg ccgcagcctt cgcctggcgc cgggcgccac agcgccggag ccgctgcgca
     6061 agctggccgg gcgcgtcgca ttccggcgcg acaccggcgc cgtcagctgg gcgctcgccc
     6121 cggcggacac ggcgacgctg gaattcagcg gcgatctcga ggcggatcgc tggcgcgtgc
     6181 gcctggacgg ccgggagtgg ccgctggcct cgctggcgtc ctttgcagcg ctgctgggcc
     6241 ggccactgcc cgtgctcggc ggcagcgccg atttttcgat cacggcgcgc ggtgccggcg
     6301 agcagctcga gggggcggtg ttcgagctgc gcgccgctgg tgtcggcggc gccaacgacg
     6361 ccggcacgat ggcggccgag gatctccggc tcgaactaaa gggctcggcc tggcgcgtcg
     6421 acgacgcgct ggccttcgat gcgcgcggca cgatcactgc cggcgaagcc tatgtcgagc
     6481 ccgtctacac cgatctctcg acgcatcccg tgcgactcaa cctgcgcggg cgcctgcacg
     6541 gcgagcggct ggagatcagc cggctggtgg cggtgcagga cgacgttctc cacctcgacg
     6601 cccgtggcga gctgctacgc acggccgctg acggctggcg gccgggcggg ggccggctgc
     6661 ggctggcgga agccgccttg ccgggcgcct atgtcacctt cctgcagccc ttcctggctg
     6721 gcaccccgct cggcgacctg gaaacctccg ggctgctgcg cggcgaagtc gagtacaccg
     6781 ccgacgggat cgcggcagtg tggctggagc tggccggcgt caatctcgac gaccgtaccg
     6841 cccggctggc cgtctacgag ctgaacggcg agctggcctg ggcgccggcc cgggacggcg
     6901 gcatgacggc gcgcgcgcgg ccggccgacc tgcgctggtc gggcggcttc gtctacggca
     6961 tcccgttcgg cgccgcgcga ctgcgcatgg actcctgggg cgggcgctgg tcactgccct
     7021 tcccggtctc cattccggta ctcgacggcg cactggagat cgagcagctc gagttcgggg
     7081 atttcatggc cggcgacgac gacatccggt tcgaggcgcg cctggcgccg atcagcatgc
     7141 gcgagctgtc gcgtgcgctc gactggccgc cgctgtccgg ccggctgtcc ggggccctgc
     7201 cccgcctgag ctaccaggac ggcgtgttga gcctcggcgg cgacctgagc gccgagatgt
     7261 tcagcggttc ggtggcggtg cgcaacctgc gcatcgagcg gccgctgcaa cccctggcgc
     7321 gcctgcaggc ggacatcgag atccggggcg tcgagctggc cgaactcacg caggcgctgt
     7381 ccttcggact catgaccggg cggctggacg gctacgtgcg cggcctggag atgatcgact
     7441 gggcgccggt cgccttcgac gcccgtctct acaccccgcc cgacagccgg gcgcgccagc
     7501 gcatcagcca gcgtgcggtg gacaacatcg cctccatcgg cggcggcggt gccggggtcc
     7561 tctccaccgg ctttctgcgc ttcttcgagg atttttccta tgcgggcttc gcgctgggct
     7621 gccggctcga gcgcgacgtc tgccggatgt cgggtctcga gacgcgcgcc gacggctacg
     7681 tcatcctgcg gggcagcgga ctgccgcgca tcgatgtgat gggctttgcg cgcgacgtga
     7741 gctggtcggc gctggtggcg cagatcgccg gcatcatggc ggcggaagac gccgaaatcc
     7801 gctgacggcg gggccgctgg gcaggtccgg acccgggcga gtatgcttag cccatggcca
     7861 tgccgcgccg aggagaattg ccatgcgtcg cctgtcgctg aaacttctgc ttgccaccac
     7921 gctgttcgcc gccgcctgcg tcaccatcaa cgtgtacttc ccggccgcgc aggccgagcg
     7981 cgccgccgac cggatcatca ccgacgtctg gggcaccgag cccgccaagc gcgacaacga
     8041 aggcgagccc cagggtcgcc tcgatccgca gcgctgggtg ccggacgccg tcgaccaggt
     8101 ccgactcgcc gcgctctcgg cgctcgactt catcgtcccg cgcgccgagg cgcaggcgga
     8161 cttcgacatc gagacgccgg agatccgcgc gctgacggcc tcgatgaacg cccgccaccg
     8221 ccagctcaag ccgagctacg actccggcgc gatcggcctc accgcggacg gcctggtgga
     8281 tatccgcgac cgcaacctgg tgccgctggc cgaacgcaac gccatccgcc agctggtgga
     8341 cgccgagaat cgcgaccgta atgcgctcta caaggccatc gcgcaggcca acggcaagcc
     8401 ggaatgggaa gccgacatcc gcaagacctt cgcgcagcgc tggatcggcc gtgccgagcc
     8461 gggctggtgg ttccggacgg ccggcggcca gtgggagcag aagtagaagt aaacgtcacc
     8521 cgcagggaag ggggagcacc ttgaacgatt tcgtgcgcat gctggctgcc tgggcgctgg
     8581 tgcacgaaag cgccgacgag ggctggaagg ccgcggtggc gcgcgggcgc aaggccgagc
     8641 ccggcgatct cgagcagggc ccggaggcct tcgtcgacgg cctcgcggcg ctggtggccg
     8701 aggaaaagga ccgcctcaag gcgcacctcg cagcgcggga cgacgccacg acgccggcgc
     8761 ggctcgacct cgacgcccgg ctcgacgcca tcgccttcga gctcgccgag ctgcgcgggc
     8821 ggctcgagtc gctgcaggcc tcgatcgacg ccatcgctgc ccgcggccgg gactagcgcg
     8881 gcatgtcgcg ccgcggccgc gccatcgccg ccgtcgcagt ccggcgcctc gcggccgcga
     8941 gctggcgtgc acgcttcggg ctgcggcgcg gccatcgccg ccgcgaagtt gtcgcacggg
     9001 aggcacgact cgcctgcgag gcgctcggcc cggccttcat caagctcggc cagctcgcct
     9061 cggtgcgccc ggacgtgttc tcccccgaga ccgtgttcga gctcgagcgc ctgcaggacc
     9121 gcattcccgc ggtgccggtc gacgagattc gcgccatcat cgccgccgag ctcggcgcgc
     9181 cgccggaggc gctgttccgg agcttcgatc cggacccgat cgccaccgcc tcgatcgccc
     9241 aggtgcactc ggccgtgctg gcgtgcgact accggccggt caccggcgcc gtgctgccgg
     9301 ccggcacgcg cctcgcgctg aaagtcgtcc ggccgggcgt ggaacgtgtc atcgccgccg
     9361 acctcgccgt ggcgcgccgc tgggccgacc gtgccgagcg cctcggacgc ttcgcccggc
     9421 tgcgcccgcg ggcgctgctg gcggagttcg agaacaccct ggcgagcgag ctcgacctgc
     9481 gcaacgaggg ccgtaccgcc gaccgcttcg cgcgcgattt tgccaccgac ccgctggtgg
     9541 tcgtgccgcg cgtggtctgg cgtcacacca cgcggcgcgt gctggcgacg gagttcgtga
     9601 acggctggcg cctcaacgac ctcggcgagg ccgagcgcag cggcatcgac gcgcggcggc
     9661 tcgcggcgca cggcgccgag gttttcatgc gccaggtgct ggtgcacggc cgctaccacg
     9721 ccgacctgca cccggccaac ctgttcgtca cgccggacgg gcggatctgc tacctggatt
     9781 tcggcatcac cgggcggacc acgccggcgc agcgcatcgc catcgcccag gtgctggcgg
     9841 ccacggtgta cggcgatgcc gagcgcgcgc tgcgctactc gcgcgaactc gggctggcga
     9901 ttccggacca ccgggagccg gccgtgcgcg cggcagtggc ggcgctcatg aagcggcatc
     9961 tcgcgggacc ggggcgtgcc gatgtgcgcg gcttcgcgct cggtttcctg tcgatgctgg
    10021 cggatttccg catcgccatc ccggaaggct acggcctgct ggtcaaggcc ctggtgacgg
    10081 tcgagggcgt ggcgcgcgcc atttaccccg acatcgacat catcgaggcc gccgggcctt
    10141 tcgcgacgcg cctgatcgcg gcgcagctgg cgcgcccgga gcgactgcgc gagcgcgtac
    10201 ccgcggcgct caaagcggcc acgcgcgagt tgctggcctg aacggcaacg aggggagacg
    10261 accgtgaaat tcctggccat cgccctgctc gtgctgctcg cctggctggc cttctggccg
    10321 gtgcccatcg agccggtggc ctgggatgcg cccgccgcac cggcgctcga gggccgctat
    10381 gctctgaacg accggcttgc cggcgtcacg cggcacggcc gggggatcgg cgtcggcccc
    10441 gaggacgtcg ccgtggatgc ggccggcaac gtgtacgtcg ggtacgagga cggccgggtg
    10501 atgcattttc cggccgggga cgcggccccg caactgctgg ccgacaccgg tgggcgcccg
    10561 ctcggcatgg aactcgacgc ggatggccgg ctggtggtgt gcgacggcca ccgcggcctg
    10621 ctgcgcatcg ccatggacgg tgccgtggag gtgctcgcca ccgcggcgga cggcctgccc
    10681 ttcggcttca ccaacgacct cgacatcgcc gccgatggcc ggatctattt cactgacgcg
    10741 tcgtggaagt tcggcccggc gatgaaggcg cgcgacgaca tcctcgagca tgggggtcac
    10801 ggccggctgc tggaacacga tcccgccacc gggcgcacgc gcgtgctgct ggacggcctg
    10861 cagttcgcca atggcgtggc gctggcgccg gacggccacg cggtgctggt ggcgcagacc
    10921 ggccgttacg atgtgctgcg ctactggctg cacggccccc tggcgggcac ccacgaagtg
    10981 ttcatcgaca acctgcccgg cattccggac ggcctttcca gcaacggccg cgacacctac
    11041 tggctggcgc tgttcaccgt gcgcaacccg gcgctggatg ccctgtcgga ctggccgtgg
    11101 ctgcgcaagg tcgtctatcg actgccgcag ttcctgcagc cgcagccggc gcgtcacgcc
    11161 ttcgtgctcg gcctgtcgct cgacggcgaa gtcgtccaca acctgcagca cgccgcaccc
    11221 gatgccttcg cgccagtcac gagcgccacg gaggcgcatg gccggctgta cctcggcagc
    11281 ctgacccagg acgccttcgc ggtgctcgac ctggcgggcc cctgagggcc cgtcgccgtc
    11341 agccgcccag caaccccgcg aacatgccct ggcggtagag caccagcagc accacggcca
    11401 gcgccagcag gaacagccag gtcttccacg ggcgcttttt ctcggcgaag gggtcctgca
    11461 gcgagcggct ggcgcctttc ggcagctcgg ccaccgcggt cagcgagccg ccgaacggca
    11521 cgttgatgcg cgcccgcgcg ttcactgccc agccgttggc gtccagcagc gggccgaggt
    11581 tgcgccggcg cagcgtcatg taggccagca gcatggacgg tccggagatg atcagcgcca
    11641 gcgccacgac caccagcggc agctgccacc aggccagctt gaacagcccg gcgacggcgg
    11701 cggccagcgc cgtgccgatg gcgcccaccg ccagcccgat ggccgcgaag atgccggcga
    11761 acttggcgat gtcgaacggc ggcgccgccg cgggctggcc cccttcgacc ttggtggccg
    11821 cgccttccac attcgcggcc gtcttgttct cgacgtcctt gtcgcgcgac gcggcgaact
    11881 tgcggatctg gttctcgatc agcgcgccga ggcgccggta cggcgtccag aaggcctggc
    11941 gaatgctgac tggctgggca accaccttca ccaccgtcgc cttccagtcg cggccttcgc
    12001 ggtcgacgaa catgccgttg cggcccggca ccatcagctc gtcgacttcg ccaccagtca
    12061 gcgcggcaac gatgtcgatc gggccgctgc cggcccgcac gcaggtgcag tacaccaggt
    12121 agcagccgct gaacggcgcc agcgtggcgt gcttcggcat gtctgagacg cgcatcacca
    12181 gctcgcagct gcgctggtcc aggtacagtg tgccggcctg gaagacggcc ttgtcggcgc
    12241 cgccgtagaa atcgctgagg gtgacgaagt tgcgcagcag cgtgaccagg tcgcgctggt
    12301 aacgcaccag ccgctcgacg gcgtcgatgt tgcccgccga ggtctcggcc ttgaggtcct
    12361 tgtcgatgag tgccgcaagg cgggcgcggg tgtcgccgga cttgagctgc tcgaggcgcc
    12421 cgagttcgag ctcgtgcacc tgcgtggccg ggcgatcggc catccaggcg cgataggccg
    12481 tgaacttcgc cgacaactcg gcccattcct gcgcggtgag cgtctcgcgc gtccccagca
    12541 gcggctcgat cacctcgtcg tggagctggg caatgcgccc cgcccaggcg ggattgacgc
    12601 ccttgccgag cggcaacggg ctccctgccg caacctgggc cagcggcagg gccgcgacgt
    12661 cggcgtcgcc ggtgtcgatc acccgctccg ccagcgcccc gtagacggtg accggggcgc
    12721 cgagcgcttc gccggcgcgg gcgtcgaacg ccgccaggcg gcagcgcgtg aaatagtcgt
    12781 ccaccttggc ctgcaccgcc tcgaaggcgc cggcggcggc cgcggtggcc tcaccgagcg
    12841 gcaggatcaa cgcggccgac tcggccgcgc gatggcgcca tgccaggacc tcgtccacct
    12901 gcgcgaaaaa tgcctccagc gtctcagccg tgatgccggg ttcgccgctg cgatcgggca
    12961 cgccgccctg ggtggccacg atctcgccga cgacctgcgc cagcgcctcg tcacccgcca
    13021 gcgccgccgg cagcacgccg tcgccgttga agtgatcggg ggtgaacagg cgggtcatgt
    13081 ccgcgaaatc ggcgacgccg agccggccgg cttccgattt gccgaggtag tccagcacct
    13141 gggtcgcggc cgccttgagc accgcaccct cggcgtggct gtcgtcgatg gcctcgaccg
    13201 gcagctcgcc gtcctcgaac agcacctgcg ggtcccggac ccgctcacag gcccagcgca
    13261 ccgcaccgag aatctccggc accctgatct ggccgtcccc gtccgtgtcc agcagcgcca
    13321 gcgtgcgggc gtcgaactcc agcccggtgg tcgggcagct caaggcggtc cacagcttgg
    13381 gatcgagctc cgccaggtgc cgcaggtcct cgccggtctc gatcgccacc tggtcgaagc
    13441 cgccgtagcg gaagaagcgc caggcgtgtg cgccgccggc tttgttcttg tcggtcattg
    13501 tggtcactcc ctgatgaaat cgagcaggtg cagcgaagcg gcgacgacgt gcagcaccag
    13561 cagcgccatc atgccccgcg cggcgagaat gtggtagccg cgccagtcca ccgcggcggc
    13621 cgagccctgc accagcagcc aggcgcagcc gctcagggcc gtcgccagca acgccagcag
    13681 caacagcccc tcgaccagcg caaacagccc gccgccctcc gcgctcggca cctcgccacg
    13741 cagcaggccg cgcagctcgc gcgccggctg cgccagcgcg ccggtgagcc agggaaaata
    13801 gagccgccag cggccgtggc gggtgcacga tgccgtaaag gtgacggcca gcaccgcggc
    13861 ggccagcccg agcaccacgt gcgcataatc cagccagccg gcgttgccgg gaattcgccg
    13921 gaacatgtgg acccacggac tggtgaggat cagccagccc gacagcagcg cgacgaacag
    13981 gtgctgtcgc tcggccaggc gggccagtcc tgcgcccatg ccgggctcag ccggggcgcc
    14041 cgtcgagcag cgcagcgaaa cgtgtcgcga agcggtcaac ttcgccccag tcggtgtact
    14101 cgtccttggt cgcaggatcc gtggggccct tggtcaacca catgatgaag cggatgacgt
    14161 tgcgatcgaa ggccgtgtag cgctggtaat cgaggtcgcc accgaacacg ccgagcagtt
    14221 tcggctgcca cgggctcttg gcgacgaaag ccttggtgta gggattcgtc tcgggcgtgt
    14281 tcttggccgg cttgcgcgcc accaggttga cggagaagaa ggcgctgggc ctggcgtcga
    14341 gcaggtcctt gtggcgggcg atgaagtcca gcaccgccgg gttgtgcttg ccgttgcgga
    14401 tgctcgcgcc gaccaggatc gcgtcgtagc gcgccgggtc cagcgcgccg tcggcgaccg
    14461 gcacggcctc gacctggtgg ccgagctcga ccagccgcgc ctggatgcgg tcgcagatct
    14521 tccgggtgtg gccgtagacg ctggaataca gcagcagaat gttggccatg gacggtcctt
    14581 cctggcgccg gggatcgctt ggattctacc ggaacggcac cagcgccacg cccgtatgtt
    14641 ccgcaacccg cctcagcgcg ccccgagcac ctcgccgagc gcctcgaggc agagcttcac
    14701 gttggcgggc cgtgcgctgt agcccatcag cccgatgcgc caggccttgc cggccagcgc
    14761 tcccagcccg ccgccgatct ccaggttgaa ctcctcgagc aggcgctggc gcgcggccgc
    14821 ctcgtccacc ccgaccggca cccgcacggc gttgagctgc ggcagccgcc agggagcgtc
    14881 gacgatgaac tccaggccca tcgcctcgag gccggcgccg agcgcctcgt gcatggcgcg
    14941 gtggcgcgcc cagctcgcct ccagcccctc ctccgccagc atcaccagcg actcgtgcag
    15001 cccgtagagc gcattgatcg gtgcagtgtg gtgataggcg cgtttcgggg tggcgtcgcc
    15061 actgccccag tagcccatca ccaggttgag gtccaggaac cagctctgca ccttgtgggt
    15121 gcggccgcgt atacgctcca ccgcgcgcgg cgagaagctc accggcgaca gccccggtgt
    15181 gcaggacagg cacttctgcg agccggaata caccgcgtcc acgccccagg cgtcgacctc
    15241 caccgggatg ccgcccagcg aggtcactgc gtccatgatc accaggcagc cgtgctcgtg
    15301 cgccagccgg cacagggtct cggcgtcgga gcgcgcgccg gtcgaggtct cggcatgcac
    15361 gaaggcgagg atgctcgcct ccggatgctc cttcagggcc agcgcgacct tgtcgggatc
    15421 gaccgggtgg ccccaggggt cctccaccac caccgcggtc gccccgcagc gctcgacgtt
    15481 ctccttcatg cgcccgccga acacgccgtt gatgcagacg atcacggtgt cgccgggctc
    15541 gaccaggttg acgaaggccg cctccatgcc cgccgagccg ggcccggaga ccgggatggt
    15601 caaggggttg gtggtacgaa aggcgtactg caggagctgc ttgagctcgt ccatcatggc
    15661 gacgaactgc gggtcgaggt ggccgatggt cggccggccc atggcctcga gcacccgcgg
    15721 gtgcacgtcc gagggaccgg ggcccatcag ggtccgcacg gggggatgga aagagtggaa
    15781 tgtcatgccg tggctccgtc gggtttggca gctacaggaa caggtccggc aacagggcct
    15841 tgctgcccgg caccaccgag tacttgtcca gatccgtgac gccgacggcg cgcagcacct
    15901 cgtcgtcgat gaagaaattg ccggtggtgg ccgcggcgtc cgccgtcagc accgcatggg
    15961 ccgcgtcggc catgatcgcc ggcgtgcggc aatgctccgg cttcacgccg ggaatcatct
    16021 gcagcgccgc ggtatcgatc accgtgcgcg gccacagcgc attgaccgcg atgccgaagg
    16081 gcttgaactc ggccgagtgc ccgaggacgc acatgctcat gccgtacttc gccatggtgt
    16141 aggccacgtg cggcgcgaac cacttcgcgt ccatgttgag cggcggcgac agcgtcagca
    16201 cgtgcgggtt gcgaccggcg gccgcgctct gcttcaggtg cggcaggcag gcctgcgtca
    16261 ccaggaaggt gccgcgcatg ttgacgccca tcatcaggtc gaagcgccgg atcggcgtcg
    16321 cctcggtcgg cgtcaggttg atggcgctgg cgttgttgac cacgacatcg atgccaccga
    16381 agcgctccac cgcggcctcc accgccccgg cgacctggcg ctcatcg
//
LOCUS       DAOMPG010000025        16228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1557_length_16228_cov_4.224510, whole genome shotgun sequence.
ACCESSION   DAOMPG010000025 DAOMPG010000000
VERSION     DAOMPG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_16228_cov_4.224510"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLS34_02360"
     CDS             complement(<1..768)
                     /locus_tag="PLS34_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRP86350.1"
                     /translation="MAASKTVGDVLRANGVSRRGFMKFCAATASMMALPPSMVSAVAA
                     AIEKARRPSVLWLPFQECTGCTESLTRSHTPTVEGLIFDAISLDYHHTLQAAAGQRAE
                     DALYQAMEENYGNYLLLIDGSIPTGDYEAYSCTNGHTHLSMLKEAAAGAAAIVTIGTC
                     AAFGGIARANPNPTQAVGVRDLIKDKPIINVPGCPPIPTVITGVLAHYLTFGTIPELD
                     KWGRPMAFFGQNIHDRCYRRPFYDRGLFADTFDDEGAK"
     gene            complement(825..1250)
                     /locus_tag="PLS34_02365"
     CDS             complement(825..1250)
                     /locus_tag="PLS34_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HRP86351.1"
                     /translation="MSGLAGIGVKVESGGGLEPELRGNALPLLHEIRHALARLLDEGE
                     PTVIDIQSIPMGPGDLRRLFDALGEGEVRAELESLGKTVIRESRYSGVWIIEHMNASG
                     GIASRFVEITWIPSLLQAQAEDVQKGLKELADALASAGN"
     gene            complement(1391..1897)
                     /locus_tag="PLS34_02370"
     CDS             complement(1391..1897)
                     /locus_tag="PLS34_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HRP86352.1"
                     /translation="MTELAHEQSGTLVLGVGNTILTDEGVGPWVVERLRALNPEAPGI
                     HWMDGGTLSFSIAAFVEDAEHLIVVDATELRADPGTVKVFEDEDMDRMLGGHGRSIHE
                     VGLMDLMNMARLTERLPRRRALVGIQPEIVDWGTAPTAAVGAAMPVAARAVAELIGRW
                     TGTEIKAE"
     gene            2045..2278
                     /locus_tag="PLS34_02375"
     CDS             2045..2278
                     /locus_tag="PLS34_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRP86353.1"
                     /translation="MCLGIPMKVVSKDGFLARCEAKGVQRDVSLFMMQDEPVEVGEFV
                     MVHVGYAIQKMTEQEARSAWEVYDQMVLNEAGA"
     gene            2282..2653
                     /locus_tag="PLS34_02380"
     CDS             2282..2653
                     /locus_tag="PLS34_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRP86354.1"
                     /translation="MHELSLCQALVAQLESVSAANGGGAVELVRLRIGPLSGVEAPLL
                     ERAFPLAAAGTIAAGAELRIANAPLVVQCTECGAQSDALPNRMLCGSCGGFRVRTVSG
                     DEMLLESVELAAPGPASAEMR"
     gene            2692..3756
                     /gene="hypB"
                     /locus_tag="PLS34_02385"
     CDS             2692..3756
                     /gene="hypB"
                     /locus_tag="PLS34_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRP86355.1"
                     /translation="MCDTCGCNITDGNRYLVSPGGRLEQTADGRAAVTVLKGLLDEND
                     HQAQHNRAHFDARGILAVNLMSSPGAGKTALLEATIDALGDPARIVVIEGDLETENDA
                     ARIRAKGVEAVQIATGSACHLDAHMVHDALHRLDLGGVDLVFIENVGNLVCPASFDLG
                     QHLNVTLLSVTEGDDKPAKYPVMFRAADLMLLTKTDLLAVLDDFDTDKAEQALRLVGS
                     AAPVLHASPRKGAGIPEWVSWIEGELLAQRARIKAGPTRRPAVQAEGARLHAAEPARG
                     EHGHAHDHGADHGHAHAHSHSHEHRHGDGRIHSHAHTHTHSHAHDHAHGHDHTHGDPR
                     EHPHSHDHDHDHEHAGDGRS"
     gene            3753..4856
                     /gene="hypD"
                     /locus_tag="PLS34_02390"
     CDS             3753..4856
                     /gene="hypD"
                     /locus_tag="PLS34_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRP86356.1"
                     /translation="MSATARDWLDRIRALPLPERVRVMNVCGGHERSISMAGLRGALP
                     PQVELIPGPGCPVCVCPEEDVYHAMQIALHDPEVIVVSFGDMLRVPVNLPLKETRSQE
                     QAKAAGADVRPVASPMEAVEIALANPARRVVFFAAGFETTTAPVAAMLAGDPPDNLMV
                     LLSGRRTWPAVALLLESEDPGFDALIAPGHVSTVMGPEEWEFVVRDHGLPSAVAGFHP
                     ESLLAATYSVLRQIVEGKCFLDNCYPEVARPNGNATARRFLAETMDVVDASWRGIGVI
                     PASGYALRAHLAARDARLHFPDYDAHDRKRAGLMPKGCDCARVVLGKIYPNQCRIYGT
                     ACTPRTPIGPCMVSDEGACRIWWSNGIRTREVA"
     gene            4820..7177
                     /gene="hypF"
                     /locus_tag="PLS34_02395"
     CDS             4820..7177
                     /gene="hypF"
                     /locus_tag="PLS34_02395"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRP86357.1"
                     /translation="MVERHPDAGGGLSPARRLWITGQVQGVGFRPFLFRLARRHGLSG
                     WAKNTVGEVEVLVCGPHPALDAFMHDVVAAAPDIAAPRLRRAEDWHGEAPRDFSILPS
                     EAGAEPRIFVPPDYFTCDDCLAELADPADRRYRYPFINVQPWGTTIIDPADRRHRYPF
                     INCTQCGPRYTLIRALPYDRANTSMAAFPLCPDCRREYEDPADRRFHAEPVACPACGP
                     RLAWQDPAGDRQHGELALAACVASLAAGRLVAVKGVGGYHLVCDAASEAAIARLRRLK
                     PRPTKPLAVMFPLAGADGLDALRRAAHPGEAEAALLRSPQRPIVLVEKRAGVLPEALA
                     PGLAELGVMLPYSPLHALLLADLRRPLVATSANISGEPVLTDGDEVETRLAHLVEGCL
                     HHDRSIVRPADDPVWRTIGGRPRPLRLGRGSAPSEIDLATPVRRPVLAVGAHMKNTIA
                     LAWGHRCVVSPHIGDMGSERSMEVFEQVAADLQALYGVTAEALACDAHPGYATARWAR
                     RRGLPLREVLHHHAHASALAAELMPAGPLLVFAWDGVGFGGDGELWGGEAFLGGPGAW
                     RRMASFRPFRLSGGERAGREPWRSAAALCWEAGRDWPACPDPGGLARAAWERGLNTPP
                     TSAVGRLYDAAAALTGLCLQASYEGEGPMRLEAATQGAAPADAAALPLAMDAGGVLRA
                     DWAPLLAPLLDAGRPVAERAALFHESLAQTAVAIARALREHHQIAGIGATGGVLQNRR
                     LADRMAALFAAAGDELALPARLPANDACIAFGQVVEQAAADAQAG"
     gene            7225..8238
                     /gene="hypE"
                     /locus_tag="PLS34_02400"
     CDS             7225..8238
                     /gene="hypE"
                     /locus_tag="PLS34_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRP86358.1"
                     /translation="MALDEARIGLAHGNGGRFMRELIQEVFARHLGGGILDTDLDACP
                     LPRMPEGELVFTTDGFTVQPLEFPGGNIGSLAVHGTTNDLAVAGATPLYLSLNVFIEE
                     GLEVELLDRIVASLAAAAREAGVEVAAGDTKVLRRGEGGGLYLATTGIGVRPHGLRLS
                     MKEIRPGDQLLVSGSVGDHGICVMLAREQFGLSGDLLSDAASVLPLTRALLGFEGLRF
                     MRDPTRGGLATVAHEIARATGCSVRLSEAAIPVRDQVRSVCEMLGYDPYYLACEGRVV
                     AVLAPEDAERALAAWRGLPGGEHAAVIGQVEAEGRPVVIETELGGRRMLEELEDDPLP
                     RIC"
     gene            8256..9065
                     /locus_tag="PLS34_02405"
     CDS             8256..9065
                     /locus_tag="PLS34_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRP86359.1"
                     /translation="MSFAPGGLITLTTDFGTADPFAGILSGRIVSNFPGARIIDLSHG
                     MPRGRPDLAGFWLAQAWREFPPGTVHLAVVDPGVGTARGVLLALAGQHALLAPDNGLL
                     TEALRGCADVAWRRMDAGLPARLGLGALSRTFHGRDLFAPLAAALASGRLAPADFGAP
                     AEPCDPAPLPQPRHDADGVAGRIVLADRFGNLFTNIDAARVTACRQPTVEAAGRSWPL
                     VSTYGDVPPGAPLALLNAFGLLELAIHGGSAAELPGLAEGAFVRLRDGLSA"
     gene            9116..11164
                     /gene="fusA"
                     /locus_tag="PLS34_02410"
     CDS             9116..11164
                     /gene="fusA"
                     /locus_tag="PLS34_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRP86360.1"
                     /translation="MKNDRTDAIRNLALIGPAGTGKTTLAEALLQASGAIKQRGRTDR
                     GDTVCDFDPREIAMGHSLDTAICHCKWQDTEINIIDTPGFTDFLPRATAVLPAVECAA
                     LVLSASGGLETGVRRMMKAAGDAELGRLIIVNKMDSEPDQLGRLLEDIQEAFGARCLP
                     VNLPTGHGKGVADCFFRDSGDTADFSSVAEAHTRILDQVVEVDEALMEAYLESGQNLD
                     PQQLHEAFEKALRERHLIPVIFVSAETGAGIEALLDYIVKLLPSPREANPPPFMRGEG
                     ESAEPVAVSAEPDQHVVAHVFKVVVDPYVGRTAVVRVHQGTIRTGAQLFVGDSRKPVK
                     LNHLYRLQGKQTAEVVEAGPGDIIAVTRIENLNFNDVLHDSHDEDHFHVRLPEAAPPM
                     MGIAIKPAQRGNEQKLSDALHKLCAEDPALRVEHRPATNETVVLCSGELHLRLVVDAM
                     KERFNVEVETHPPTIPYRETITRPAEGHHRHKKQTGGAGQFGEVYLRIAPLPRGSGFE
                     FVSEVVGGTIPQQFIPAVEKGVRQALESGAIAGFPLQDIRVAVYDGKTHPVDSKEVAF
                     ISAGRRAFIEAIKNAGAIVLEPIVQLQVTVPFDSLGAVTGHLSSRRGRMLGSTSLSGQ
                     RIQIDAEAPLAELQDYANQLKAMTGGSGGWSMQLDRYEPVPPKVQQELVNAARTEESD
                     "
     gene            11258..11695
                     /locus_tag="PLS34_02415"
     CDS             11258..11695
                     /locus_tag="PLS34_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP86361.1"
                     /translation="MLDISNIMTPRHSLVIITPAATVDEARRTMAAHAIRHLPVVDEA
                     GGLVGLVSQTDVLVAGGDGARPVGEIMVREVDTVDERSNVRHAALLMFRHKRSCLPVL
                     RDGALRGIVTDADFLGVAIALMEQLEAAEPEPEDTEVADDVAS"
     gene            complement(11713..12909)
                     /locus_tag="PLS34_02420"
     CDS             complement(11713..12909)
                     /locus_tag="PLS34_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRP86362.1"
                     /translation="MSAISDEALRRFGLHYAPFEPESAGLSFYVGPQHREALGFLERA
                     LHSNDLLVALTGEPGSGKRATMDFALHQTMPGALVARLGPLTADPDEFLAALLGGFGF
                     EGVKASRDEMRGLLTVFLGHQRQKGVTTVIVADNPEVLSGGIVEEVGWLALLEPVRLG
                     RLKLVLLGGDTLEHQLAAPRMHAIRQMIRWQHRLEPLGIDETRDYLEFHAESAGCAHP
                     GDLFSPEAVARIHALSGGLPGRINQLAAQAMSAAAAAAESMVGGKHVQPVPAGKAGEE
                     KPRTRRIASLDILLDQEPKARIRLSASRLLIGRHPWNDVQLDHDSVSRYHAMLVREGG
                     HWTVVDLNSTNGIRVNEREVRQQRLRHGDIVQIGRYRLVLNEGVAPARNLPAMGDVSE
                     TTVIGG"
     gene            complement(12906..13280)
                     /locus_tag="PLS34_02425"
     CDS             complement(12906..13280)
                     /locus_tag="PLS34_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRP86363.1"
                     /translation="MDCPKCGHAMEQVVYADIEVDRCTLCRGLWFDMLEHEQLKEIHG
                     AESIDTGDREVGAVFNRDDRIRCPRCDGLMIRMVDAAQPHIWYESCGTCHGVFFDAGE
                     FSDYKRHTLADLFRRLRARERR"
     gene            complement(13347..13766)
                     /locus_tag="PLS34_02430"
     CDS             complement(13347..13766)
                     /locus_tag="PLS34_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP86364.1"
                     /translation="MKYLHTMVRVSDLDASLDFYCNKLGLVELRRRDSEAGRYTLVFL
                     AAPGDERAQVELTFNWDPEEYGSARNFGHLAYEVEDIYATCQRLMDGGVVINRPPRDG
                     RMAFVRSPDRISIELLQKGEALPPREPWVSMPNSGEW"
     gene            complement(13763..14842)
                     /locus_tag="PLS34_02435"
     CDS             complement(13763..14842)
                     /locus_tag="PLS34_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRP86365.1"
                     /translation="MDVILAWLRRQLENPQVVALALIVLVLAAAIWLFGKMLAPLLAS
                     LVIAYLLNGIAARLRARGVPNIVAVLFVFLLFLAILFFCFFWLLPLLTRQAAQLLQQV
                     PLMVGEVQRALLQLPERYPAMVSEQQIKEVFAGFNSELIRFGQRLVSFSVASLMTLIT
                     LLVYLVLLPILVFFLLKDKDLLIGWAAGFLPKDRHLTHEVWVEVDKQIGNYVQGKLVE
                     ILIVGLVAWIAFRFLGLQYAVLLAAATGLSVVIPYIGATVVTLPVALVAYFQFGLAPQ
                     FYYVLLAYGVIQFLDGNVLVPVLFSEVVDLHPVAIIAAILLFGGLWGFWGIFFAIPLA
                     TVIQAILRAWPRQVAYAPPVEDQGK"
     gene            14969..15289
                     /locus_tag="PLS34_02440"
     CDS             14969..15289
                     /locus_tag="PLS34_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86366.1"
                     /translation="MHWIRREALLLLIFGGFGLLGLPALVYLVGQVLLGEYRPGAGMG
                     TFYADLYGHLAAASPWAWLLVLGPYLAIQLLRLLWLPLGLLTHRPARRERRAKVDERI
                     EPTI"
     gene            15346..15912
                     /locus_tag="PLS34_02445"
     CDS             15346..15912
                     /locus_tag="PLS34_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRP86367.1"
                     /translation="MSLKVTFELADADLDHFRRVMRQARSVAKTRGPDEVLAAAEALL
                     GRIKGIEIPEFIRDRLERLRMLIDMLRDVEWKLPEPEATRVLNALAYFCEAEDLIPDN
                     VPGLGFLDDAIMVELVVRELRHEIEAYQDFCSYRATRVGESADAVTREEWLSARRKAL
                     QSRMRNRRRRDRERRRGGGGGRSPFSLF"
     gene            complement(15939..16175)
                     /locus_tag="PLS34_02450"
     CDS             complement(15939..16175)
                     /locus_tag="PLS34_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86368.1"
                     /translation="MKRGSLQSGDSMHETAPYIGALRRRLRLLLPDKWFTSGARAAAA
                     MLGRRLARARVELDPDAPASSWVAWEVSRRGRPG"
BASE COUNT         2583 a         5701 c         5469 g         2475 t
ORIGIN      
        1 cttcgcgccc tcgtcgtcga aggtgtcggc gaacaggccg cggtcgtaga acggccgccg
       61 gtagcagcgg tcatggatgt tctgcccgaa aaaggccatc ggccgacccc acttgtccag
      121 ctcggggatg gtgccgaagg tcaggtagtg cgccagcacg ccggtgatca cggtcgggat
      181 cggcgggcag ccgggcacgt tgatgatcgg cttgtccttg atgaggtccc gtacgccgac
      241 cgcctgggtc ggattcggat tggcccgggc aatgcccccg aaggcggcac aggtgccgat
      301 ggtcacgatg gcggccgcac ccgcagcagc ttccttgagc atcgacaggt gcgtatgccc
      361 gttggtgcag gagtacgcct cgtagtcacc cgtggggatg gaaccgtcga tcagcaacag
      421 gtagttgccg tagttctcct ccatcgcctg gtacagggcg tcctcggcgc gctggccggc
      481 ggccgcctgc agggtgtgat gatagtcgag cgaaatcgca tcgaaaatca ggccctcgac
      541 ggtgggggtg tgcgaacgcg tcagcgattc ggtgcagccc gtgcactcct ggaacggaag
      601 ccagagaacc gacggcctcc tggccttttc gattgccgct gccacggcgc tgaccatgct
      661 tggcggcaac gccatcatgg acgcggttgc cgcgcagaat ttcatgaacc cgcgacggct
      721 gactccgttc gcccggagga cgtcgcctac ggtcttgctt gctgccatgc tgtgctcctc
      781 cgcactgccc cgcctttcac gttcacaggg accttcggtt ggcttcaatt accggccgac
      841 gccagcgcgt cggcaagttc cttgagacct ttctggacgt cctcggcctg cgcctgcagg
      901 agcgatggaa tccaggtgat ctcgacaaaa cggctggcga tcccgccaga cgcattcatg
      961 tgctcgatga tccagacgcc ggaatagcgc gattcgcgaa tgacggtctt gccgaggctt
     1021 tccagctcgg ccctgacctc gccctcgccc agcgcgtcga acagccggcg caggtcgccg
     1081 gggcccatgg gaatgctctg gatgtcgatg acggtgggct cgccttcgtc gaggagacgc
     1141 gcgagcgcat gacggatttc gtgcaacagg ggcagcgcgt taccgcgaag ctcgggttcc
     1201 agtccgccgc ccgactccac cttgacccca attcccgcga gaccgctcat ggcgcctccg
     1261 ccgatggcgg ccagtgtcca tccgctggac ccgtccgcat cggtcttcgt cgttgcttca
     1321 ataccttacc gggcaatcta tgcgtccggc cgtcacccag caatacgcaa ttcacacatc
     1381 agtagcgggc tcattccgcc ttgatttcgg tgcccgtcca gcggccgatc agctcggcca
     1441 cggcgcgcgc cgccaccggc atcgccgcgc ccaccgcggc cgtcggcgcg gtgccccagt
     1501 cgacgatttc cggctggatg ccgaccagcg cgcggcgcct gggcaggcgc tccgtgaggc
     1561 gcgccatgtt catcaggtcc atgaggccca cctcgtgaat gctgcggccg tgcccgccga
     1621 gcatccggtc catgtcctcg tcctcgaaca ccttgacggt gccgggatca gcgcgcagct
     1681 cggtcgcgtc cactacgatc aggtgctcgg cgtcctcgac gaacgcggcg atcgagaagc
     1741 tcagcgtgcc gccgtccatc cagtggatgc cgggcgcttc gggattcagc gcccgcagtc
     1801 gctcgacgac ccaggggccg acgccctcgt ccgtcaggat ggtgttgccg acgccgagta
     1861 ccagcgtgcc gctctgttca tgtgccagtt cagtcatgaa tcctgctccg cgctgtccgg
     1921 accggggcgc cgctgcagaa tacccggatg agaactggac gcatcaggga atctacgggc
     1981 ttgcaccgct gcggccgcgg tggcagcttg atgccctgcg gagaagcgtg tcggagcaaa
     2041 gaatatgtgc ctgggcattc ccatgaaggt ggtctcgaag gatggcttcc tggcccgctg
     2101 cgaggccaag ggcgtccagc gggacgtcag cctgttcatg atgcaggacg agccggtcga
     2161 ggtcggtgag ttcgtcatgg tccacgtcgg ctacgccatc cagaagatga ccgagcagga
     2221 ggcgcgctcc gcctgggagg tctacgacca gatggtgctc aacgaagcgg gcgcctgaac
     2281 cgtgcatgag ctgtcgctct gccaggcact ggtagcccag ctggagtcgg tctccgcggc
     2341 caacggcggc ggcgccgtgg agctggtcag gctgcgtatc ggccccctct cgggggtgga
     2401 ggcgccgctg ctggagcgag ccttcccgct ggccgccgcc ggcaccatcg cggcgggcgc
     2461 cgaactacgc atcgccaacg ccccgctggt cgtgcagtgc accgagtgcg gggcgcagag
     2521 tgatgcgctg ccgaaccgga tgctgtgcgg cagctgcggt ggattccggg tgcgcaccgt
     2581 gtcgggggac gagatgctgc tcgagtccgt cgagctcgcc gcgcccggcc cggcgagcgc
     2641 ggaaatgcgc taaagttctc attctcattt acatttttcc tggagttcgc catgtgcgat
     2701 acctgcggct gcaacatcac tgacggcaac cgctacctgg tctcccccgg cggccggctg
     2761 gaacagaccg ccgacgggcg cgcagccgtc accgtgctga agggcctgct cgacgagaac
     2821 gaccaccagg cgcagcacaa ccgggcgcat ttcgacgcgc gcggcatcct cgccgtgaac
     2881 ctgatgtcgt cccccggggc cggcaagacc gccctgctgg aggcgaccat cgatgcgctc
     2941 ggcgacccgg cgcgcatcgt ggtcatcgag ggcgacctcg agacggagaa cgacgccgcc
     3001 cgcattcgcg ccaagggcgt ggaagccgtg cagattgcca ccggcagcgc ctgccacctg
     3061 gacgcccaca tggtccacga cgccctgcac cggctggacc tggggggcgt cgacctggtg
     3121 ttcatcgaga acgtcggcaa cctggtgtgc ccggccagtt tcgacctcgg ccagcatctc
     3181 aacgtgaccc tgttgtcggt caccgagggc gacgacaagc cagccaagta cccggtgatg
     3241 ttccgcgccg cggacctgat gttgctgacc aagaccgacc tgctggcggt gctggacgat
     3301 ttcgacaccg acaaggccga acaggcgctg cgcctggtcg gctcggccgc gccggtcctg
     3361 catgcatcgc cgcgcaaggg cgccggcatc cccgagtggg tttcgtggat cgagggcgaa
     3421 ctgctggccc agcgcgcgcg catcaaggcc gggcccaccc gccggccggc ggtgcaggcg
     3481 gagggcgcgc gactgcacgc cgccgagccg gcgcgcggcg aacacggcca tgcccatgac
     3541 cacggcgcgg accacggcca cgcgcatgcg cactcccaca gccacgagca ccgccacggc
     3601 gacgggcgca tccactcgca tgcccacacg cacacccaca gccacgccca cgaccatgcc
     3661 cacgggcacg accacacgca cggcgacccg cgcgagcatc cgcacagcca cgatcacgac
     3721 cacgaccacg agcacgccgg ggacggccgc tcgtgagcgc cacggccaga gactggctcg
     3781 accgcatccg ggcgctgccg ctgccggagc gcgtgcgcgt gatgaacgtc tgcggtgggc
     3841 atgaacgctc catttccatg gccggcctgc gcggcgccct gccgccccag gtcgagctga
     3901 tcccgggccc gggctgcccg gtgtgcgtct gccccgaaga ggacgtctat cacgctatgc
     3961 agatcgcgct gcacgatccc gaggtcatcg tcgtcagctt cggtgacatg ctgcgcgtgc
     4021 cggtcaacct gccgctcaag gaaacccgca gccaggaaca ggccaaggcg gccggtgccg
     4081 acgtgcgccc ggtggcctcg cccatggaag cggtggagat cgcgctggcc aacccggccc
     4141 ggcgggtggt gtttttcgcc gccggcttcg agacgaccac cgcgccggtg gcggccatgc
     4201 tggcggggga cccgcccgac aacctgatgg tgctgttgtc gggtcgccgg acctggccgg
     4261 ccgtcgccct gctgctcgag tccgaggatc cgggcttcga tgcgctgatc gcccccgggc
     4321 atgtctccac cgtgatgggc cccgaagagt gggagttcgt cgtcagggac cacggcctgc
     4381 cgtcggcggt ggcgggcttc cacccggaga gcctgctggc cgcgacttac tcggtgctgc
     4441 gccagatcgt cgagggcaag tgctttctcg acaactgcta cccggaggtc gcacgcccga
     4501 acggcaacgc caccgcgcgc cgcttcctgg ccgagaccat ggacgtcgtg gacgcctcgt
     4561 ggcggggcat cggcgtcatt ccggcctccg gctacgcgct ccgggcgcac ctcgcggcgc
     4621 gtgacgcccg actgcatttc ccggactacg acgcccatga ccgcaagcgc gccggactga
     4681 tgcccaaggg ctgcgattgc gcccgcgtgg tgctgggcaa gatctatccc aaccagtgcc
     4741 ggatctacgg caccgcgtgc acgccgcgca cgcccatcgg tccctgcatg gtgtcggacg
     4801 aaggcgcctg ccgtatctgg tggtcgaacg gcatccggac gcgggaggtg gcctgagccc
     4861 cgctcgccgg ctctggatca cgggacaggt ccagggggta ggctttcgtc cgttcctgtt
     4921 tcggctggcc cggcgccacg gcctctcggg ctgggcgaag aacaccgtcg gcgaagtcga
     4981 agtgctggtc tgcggcccgc atccggcgct cgacgccttc atgcacgacg tggtcgctgc
     5041 cgccccggac atcgccgcgc cgcggctgcg gcgcgccgaa gactggcacg gcgaagcacc
     5101 gcgggacttc agcatcctgc cgagcgaggc cggcgccgag ccgcgcatct tcgtgccgcc
     5161 ggactatttc acctgcgacg actgcctggc ggagcttgcc gacccggccg accgtcgcta
     5221 ccgctacccc ttcatcaacg tgcagccgtg gggcaccacg atcatcgacc cggccgaccg
     5281 ccgccaccgc tatccattca tcaactgcac ccagtgcggc ccccgctaca ccctgatccg
     5341 cgccctgccc tacgatcgtg ccaacacctc gatggcggcc ttcccgctct gcccggactg
     5401 ccgccgtgaa tacgaggatc ctgcagaccg ccgcttccat gccgagccgg tggcctgccc
     5461 ggcctgtggt ccgcgcctgg cctggcagga tcctgctggc gaccgccagc acggcgagct
     5521 cgcgctggcc gcctgcgtag ccagcctggc cgcggggcgg ctggtcgcgg tcaaaggggt
     5581 cggcggctac cacctcgtct gcgacgccgc cagcgaggcg gccatcgcgc ggctgcgacg
     5641 cctgaaaccg cgtcccacca aaccgctcgc cgtgatgttc ccgctggcag gcgccgacgg
     5701 cctcgacgcg ctgcgccgcg ctgcgcaccc gggcgaagcc gaggccgcgc tgctgcgatc
     5761 gccgcagcgc ccgatcgtgc tggtggaaaa gcgcgccggc gtcctgcccg aggcgcttgc
     5821 gccaggactc gcggagctcg gcgtcatgct gccctacagc ccgctgcatg ccttgttgct
     5881 ggcggacctg cggcggccgc tggtcgcgac ctcggccaac atcagcggcg agcccgtgct
     5941 gaccgacggc gacgaggtgg agacccgcct cgcgcacctc gtcgagggct gcctgcatca
     6001 tgaccggtcg atcgtccggc cggccgacga cccggtctgg cgcaccatcg gcgggcggcc
     6061 gcgcccgctg cgcctcggcc gtggcagcgc gccgtccgaa atcgacctgg cgacacccgt
     6121 ccgccggcct gtattggcgg tcggcgcaca catgaagaac accatcgcgc tggcctgggg
     6181 ccatcgctgc gtcgtctcgc cgcacatcgg cgacatgggc tcggagcgca gcatggaagt
     6241 attcgagcag gtagccgcgg atctgcaggc gctctacggc gtcacggccg aggccctcgc
     6301 ctgcgatgcg catcccggct atgccaccgc ccgctgggcc cgccgccgcg gcctgccgct
     6361 gcgggaagtg ctgcaccacc atgcccatgc ctccgccctg gcggccgagt tgatgccggc
     6421 cggcccgctg ctggtatttg cctgggacgg cgtcggcttc ggcggcgacg gcgagctatg
     6481 gggcggcgaa gcctttctcg gcggcccggg ggcctggcgc cggatggcca gtttcaggcc
     6541 gtttcggctg tcgggcgggg agcgcgcggg gcgcgagccc tggcgcagcg cggcggccct
     6601 gtgctgggaa gccggccgcg actggcccgc atgtcccgat cccggcgggc tcgcccgggc
     6661 ggcctgggaa cgtggcctga acacgccacc cacgagcgcc gtcgggcggc tgtacgatgc
     6721 cgccgccgcg ctgaccggcc tgtgcctgca ggccagctac gagggtgaag ggccgatgcg
     6781 cctcgaggcg gcgacgcaag gcgcggcgcc ggccgacgcc gcggcgctgc cgctggccat
     6841 ggatgccggg ggagtattgc gggccgactg ggcgccgttg ctggcgccgc tgctcgacgc
     6901 gggccgcccg gtggcggaac gcgccgcgct gtttcacgag agcctcgccc agacggccgt
     6961 ggcgatcgcc cgggcactgc gcgagcacca ccagatcgcc gggatcggcg ccaccggcgg
     7021 ggtgctgcag aaccggcgac tcgccgatcg catggcggcg ctgttcgccg cggccggtga
     7081 cgagctggcg ctgccggcac gactgccggc caacgacgcc tgcatcgcct tcggacaggt
     7141 cgtcgagcag gccgcagcgg atgcgcaagc cggttagaat ggcaggtcga ggcaacccac
     7201 cgaggccgac gaggagattc ctgcatggcg cttgacgagg cacgcatcgg gctcgcccat
     7261 ggcaacggcg gccgcttcat gcgggaactc atccaagagg tgttcgcccg ccatctcggc
     7321 ggcggcatcc tcgacaccga cctggacgcc tgtcctctgc cccgcatgcc cgagggcgaa
     7381 ctggttttca cgaccgacgg tttcaccgtg cagccgctgg agtttcccgg cggcaacatc
     7441 ggctcgctag cggtacacgg caccaccaat gacctcgccg tggccggcgc cacgccgctg
     7501 tatctctcgc tcaacgtgtt catcgaggaa ggcctcgagg tcgagttgct cgaccgcatc
     7561 gtggcgagcc tggccgcggc ggcgcgcgag gccggcgtcg aagtcgcagc cggagacacc
     7621 aaggtgctgc ggcgcggcga gggcggcggc ctgtacctgg ccaccaccgg catcggcgtg
     7681 cgtccccacg gcctgcggct gagcatgaaa gagatccgtc ccggcgacca actgctggtg
     7741 agcggcagtg tcggcgacca cggcatctgc gtgatgctgg cgcgtgagca gttcgggctg
     7801 agcggcgacc tcttgtccga tgcggccagc gtgctgccgc tgacgcgtgc cttgctggga
     7861 ttcgagggac tgcgcttcat gcgtgatccg acgcgcggcg ggctggcgac cgtggcccac
     7921 gagatcgccc gcgccacggg ctgctcggtg cgcctttccg aggccgcgat cccggtgcgc
     7981 gaccaggtgc gcagtgtctg cgagatgctg ggctacgatc cctattacct ggcctgcgaa
     8041 ggccgggtcg tggcggtact cgcgccggag gatgccgaac gggcgctggc ggcctggcgg
     8101 ggcctgccgg gcggcgagca tgctgccgtc atcggccagg tcgaggcgga gggacggccg
     8161 gtggtcatcg aaacagagct cggcggccgc cgcatgctcg aggaactgga ggatgatccg
     8221 ctaccgcgga tctgctgaac acacaggcgg gaaacatgtc tttcgccccg ggcgggctga
     8281 tcacgctcac gacggacttc ggcacggcgg accccttcgc cggcatcctc agcgggcgca
     8341 tcgtgagcaa ttttcccggc gcccggatca tcgacctcag ccacgggatg ccgcgcggcc
     8401 gaccggacct ggccggcttc tggctggcgc aagcctggcg cgaatttccg cccggcacag
     8461 tccatctcgc agtagtcgat ccgggcgtcg gcacggcgcg cggcgtgctg ctggcactgg
     8521 ccggtcagca cgccctcctc gcaccggaca acggcctgct caccgaggca ttgcggggct
     8581 gcgcggacgt ggcatggcgc cgcatggacg ccggcctgcc cgcgcgcctc ggtctcgggg
     8641 ccctgtcgcg tactttccac ggccgcgacc tgttcgcacc gctggccgcc gcgctggctt
     8701 ccggccggct ggcgcccgcc gatttcggcg ccccggccga gccctgcgat ccagcgccgc
     8761 tgccacagcc gcggcacgac gccgacggag tcgccggccg catcgtgctg gccgaccgct
     8821 tcggcaacct gttcaccaac atcgacgccg cgcgggtcac cgcctgccgc cagccgacgg
     8881 tcgaggcggc gggccgcagc tggccgctgg tgtcgaccta cggcgacgtc ccgcccggcg
     8941 ccccgctggc gctgctcaat gctttcggcc tgctggaact ggcgatccat ggcggcagcg
     9001 cggcggaatt gccgggcctt gccgagggcg ccttcgtgcg gctgcgcgac gggctcagcg
     9061 cctgaccgaa tgatttgact cttacattgg ctgcagacac ggaaaggtcc atgaaatgaa
     9121 aaacgacaga actgacgcta tccgtaatct cgcgctcatc ggccctgccg ggaccggcaa
     9181 gacgaccctt gccgaagcct tgctccaggc atccggcgcc atcaagcagc gcgggcggac
     9241 cgatcgcggc gacaccgtat gcgacttcga tccgcgcgag atcgccatgg gccactcgct
     9301 cgatactgcg atctgtcact gcaagtggca ggacaccgag atcaatatca tcgacacccc
     9361 tggattcacc gacttcctgc cgcgcgccac cgcagtgctg ccggcggtgg aatgcgcggc
     9421 acttgtgctg tccgcctccg gcggcctcga gaccggtgtg cgacggatga tgaaggccgc
     9481 cggcgacgcc gagctcgggc ggctgatcat cgtcaacaag atggactcgg agccggacca
     9541 gctcggccgg ctgctggagg acatccagga ggctttcggg gcacgctgcc tgccggtcaa
     9601 cctgccaacc ggtcacggca agggcgtggc cgactgcttc ttccgcgaca gcggcgatac
     9661 cgcggacttc tcgagcgttg cggaggcgca tacccgcatc ctcgaccagg tcgtggaagt
     9721 ggacgaggcg ttgatggagg cttaccttga atccggccag aacctggatc cgcaacaact
     9781 gcacgaggcc ttcgagaagg cgctccgcga gcggcacctc attcccgtaa tcttcgtgtc
     9841 ggccgagacc ggcgccggga tcgaagccct gctcgactac atcgtgaagc tgctgccgag
     9901 cccgcgcgag gcgaacccgc cgccgttcat gcgcggcgaa ggcgaatccg ccgagccggt
     9961 cgcggtgtcc gcggagcccg accagcacgt cgtcgcccac gtcttcaagg tggttgtgga
    10021 tccctacgtg gggcgcaccg ccgtggtgcg cgttcaccag ggcaccatca ggaccggcgc
    10081 gcagctgttc gtcggcgatt cacgcaagcc ggtgaagctt aatcaccttt accgcctgca
    10141 aggcaagcag accgccgagg tcgttgaagc gggccccggc gacatcatcg ccgtgacccg
    10201 catcgagaac ctgaatttca acgacgtgtt gcacgactcg cacgacgagg accacttcca
    10261 cgtgcggctg ccggaggccg cgcccccgat gatgggcatc gccatcaagc ccgcgcaacg
    10321 cggcaacgaa cagaaactgt cggatgccct gcacaagctg tgtgccgagg acccggcgct
    10381 gcgcgtagag catcgcccgg cgaccaacga aaccgtcgtc ctgtgcagcg gcgagctgca
    10441 cctgcggctg gtcgtcgacg cgatgaagga acgtttcaac gtcgaggtcg agacgcatcc
    10501 acccaccatt ccttatcgcg aaaccatcac ccggccggcc gagggccacc accggcacaa
    10561 gaagcagacc ggcggcgccg gccagttcgg cgaggtctac ctgcgcatcg cgccgttgcc
    10621 gcgcgggtcg ggcttcgaat tcgtcagcga ggtggtcggc ggcacgattc cgcagcagtt
    10681 catccccgcg gtcgagaaag gcgtccgcca ggccctcgag tccggcgcca ttgccggctt
    10741 cccgctgcag gacatccgtg tggcggtcta cgacggcaag acgcacccgg tggactccaa
    10801 ggaagtcgcc ttcatcagcg cagggcggcg ggccttcatc gaggcgatca agaacgccgg
    10861 ggccatcgtg ctcgagccca tcgtccagct gcaggtcacg gtgcccttcg actcgctcgg
    10921 cgcagtgacc ggccacctgt cgtcgcgccg cgggcggatg ctgggctcga cttcgctttc
    10981 cgggcagcgc atccagatcg acgcggaggc gccgctcgcc gagctgcagg actacgccaa
    11041 ccagctcaag gcgatgaccg gcggctccgg cggctggtcg atgcagctcg accgctacga
    11101 gcccgtaccg cccaaggtcc agcaggaact cgtgaacgcg gcgcgcaccg aggaatccga
    11161 ttagaatgcg aggcaggcgc cgcgggcttg acgcaggtca atgcaccgcg gcgccaccac
    11221 gccaagatgg acttacaacg cagggaaagc agagcacatg ctcgacatca gcaacatcat
    11281 gacgccgcgg cacagcctgg tgatcatcac gccggccgcc accgtcgacg aagcgcgccg
    11341 caccatggcg gcgcacgcga ttcgccacct gccagtggtc gacgaggccg gcggcctggt
    11401 gggcctggtg tcgcagaccg acgtgctggt ggccggcggc gacggcgccc ggccggtcgg
    11461 cgagatcatg gtgcgtgagg tcgacacagt ggatgagcgc agcaacgtcc gccacgcggc
    11521 gttgctgatg tttcgtcaca agcgcagctg cctgccggtg ctgcgcgacg gtgcattgcg
    11581 cggcatcgtc acggatgccg actttctcgg cgtggccatc gcgctcatgg aacagctgga
    11641 agccgcggag ccggagcccg aagacaccga agtggcggac gacgtggcgt cctgaggccc
    11701 gccgcgaccc ggtcagccac cgatgacggt ggtctctgaa acgtcgccca tggccggcag
    11761 gttgcgcgcc ggcgccactc cctcgttgag caccagccgg taacgaccga tctgcacgat
    11821 atcgccgtgg cgcagtcgct gctgccggac ctcccgctcg ttgacccgga tgccgttggt
    11881 gctgttcaag tccaccacgg tccagtgccc gccttcgcgc accagcatgg cgtggtagcg
    11941 gctgaccgaa tcgtgatcga gctgcacgtc gttccatgga tgacggccga tcagcaggcg
    12001 ggaagcgctc aaccggatgc gcgccttggg ctcctggtcc agcaggatgt cgagcgaggc
    12061 gatccgccgg gtgcggggct tctcctcgcc ggccttgccc gccggcaccg gctgcacatg
    12121 cttgccgccc accatgcttt cggcggcggc cgcggcggcc gacatggcct gggcggccag
    12181 ctggttgatg cgcccgggca accccccgga caaggcatgg atacgcgcca ccgcttccgg
    12241 gctgaacagg tcgcccgggt gggcgcagcc cgcagattcg gcgtggaact ccaggtagtc
    12301 gcgggtctcg tcgatgccca gcggctccag gcggtgctgc cagcggatca tctgccggat
    12361 ggcatgcatc cgcggcgccg caagctggtg ttcgagcgta tcgccgccga gcaacaccag
    12421 cttgaggcgg ccgagccgca ccggctccag cagcgcaagc cagcccacct cctcgacgat
    12481 accgcctgac agcacctcgg gattgtcggc cacgatcact gtggtcacgc ccttctggcg
    12541 ctggtgaccc aggaacacgg tcagcaggcc gcgcatctcg tcgcggctgg ccttgacgcc
    12601 ctcgaacccg aagccgccca gcaaggccgc caggaattcg tccgggtcgg ccgtcagcgg
    12661 cccgagccgc gccaccagcg cgccgggcat ggtctggtgc agcgcgaaat ccatggtggc
    12721 gcgcttgccc gagcccggtt ccccggtcag ggcgaccagc aggtcgttcg aatgcagggc
    12781 gcgctcgagg aagccgaggg cctcacggtg ctgcgggccg acatagaacg acaggccggc
    12841 ggactccggc tcgaacggcg catagtgcag cccgaatcgc cgcagcgcct cgtcggaaat
    12901 cgcgctcacc tgcgttcccg ggcgcgcagc cgccggaaca ggtcggccag cgtgtgacgc
    12961 ttgtagtcgc tgaactcgcc ggcgtcgaaa aacacgccgt ggcaggttcc gcaggactcg
    13021 taccagatgt ggggctgggc cgcatccacc atgcgaatca tgaggccgtc acagcggggg
    13081 cagcggatgc ggtcgtcccg attgaacacc gcgcccacct cgcggtcacc cgtgtcgatg
    13141 gactcggcac cgtgaatttc cttgagctgc tcatgctcca gcatgtcgaa ccagagcccc
    13201 cggcacaatg tgcagcggtc cacctcgata tcagcgtaga ccacctgctc cattgcgtga
    13261 ccgcatttcg gacagtccat cggcacatct ccgtcggaaa tccgaatcta ccgctgcgcg
    13321 tcccgcgcca caacaggcgc gcacaatcac cactcgcccg aatttggcat gctcacccag
    13381 ggttcgcgcg gcggcaaggc ctcgcccttc tgcagcaatt cgatcgaaat ccggtcgggc
    13441 gaacgcacga aggccatccg gccgtcccgt ggcgggcgat tgatgaccac gccgccgtcc
    13501 atcagccgct ggcaggtcgc gtagatgtcc tcgacctcat aggccaggtg accgaagttg
    13561 cgcgcgctgc cgtattcctc cggatcccag ttgaaggtca gttctacctg ggcgcgctcg
    13621 tcgccgggcg cggccaggaa caccagggtg tagcggcctg cctcgctgtc gcgccgcctg
    13681 agttcgacca ggcccagctt gttgcagtag aaatccagcg acgcatcgag gtcgctgact
    13741 cgcaccatgg tgtgcaggta cttcacttgc cttgatcctc cacgggcggc gcatacgcta
    13801 cctggcgcgg ccaggccctg aggatggcct ggatgaccgt cgccagcggg atggcgaaga
    13861 agattcccca gaatccccac aggccgccga acagcaggat cgcggcgatg atcgccacag
    13921 ggtgcaggtc gacgacctcg ctgaacagca ccgggaccag cacattgccg tcgagaaact
    13981 ggatgactcc gtaggcgagc agcacatagt agaactgcgg cgccaggccg aactggaagt
    14041 aggcaaccag cgccaccggt aacgtcacca cggtggcgcc gatgtaagga atcaccaccg
    14101 acaggccggt cgccgcagcc agcagcaccg cgtactgcag cccgagaaaa cgaaacgcga
    14161 tccaggccac cagcccgaca atgagaatct cgaccagctt gccctgcacg taattgccga
    14221 tctgcttgtc gacctcgacc cagacctcat gggtgagatg gcgatccttg ggcaggaaac
    14281 cggccgccca gccgatcagc aggtccttgt ccttcagcag gaagaacacc aggatcggca
    14341 gcagcacgag gtacaccagc agagtgatga gcgtcatcaa cgaggccacg gagaagctca
    14401 ccagccgctg gccgaagcgg atcagttcgc tgttgaaccc ggcgaacact tccttgatct
    14461 gctgctcgga caccatcgcc gggtagcgct ccggcaactg cagcagcgcc cgttgcacct
    14521 cgccgaccat cagcggcacc tgctgcaaca gctgcgcggc ctggcgggtc agcagcggca
    14581 gcagccagaa gaagcagaag aacaggatgg caaggaacag cagaaatacg aacagcacgg
    14641 ccacgatatt cggcacccca cgggcgcgca gccgcgcggc gatgccgttc agcaggtagg
    14701 cgatcaccag cgaggcaagc aacggcgcga gcatcttgcc gaacagccag atggccgccg
    14761 ccagcaccag cacgatgagc gcgagggcga cgacctgggg attctcaagc tgtcgtcgca
    14821 accacgcgag aatcacgtcc atggcaggat ccgtgtgaat tcggcgcaag gatatcaggt
    14881 cgcggcccgg ccggcgcccg cagttgtcgc ccggcagcgg gtctgtgaga atcgtgacct
    14941 ggaatcacgc tgcccggggg gccgtagctt gcactggatt cgccgcgagg ccctgctgct
    15001 gttgatattc gggggattcg ggctgctcgg tttgcctgcg ctggtctacc tggtcgggca
    15061 ggtcctgctg ggcgagtatc gtcctggcgc gggcatgggc accttctacg ccgacctcta
    15121 cggccacctg gccgcagcat cgccgtgggc gtggctgctg gtgctcgggc cgtacctggc
    15181 gatccagctg ctccggctgc tgtggctgcc gctcggcctg ctgacgcacc ggccggcccg
    15241 gcgtgagcgc agggccaagg tcgacgagcg catcgaaccc accatttgac gcttttccgg
    15301 gcgcgtcgta ctctccggct gcgcctgcca cggagtgaga gcaacatgtc cctgaaggtt
    15361 actttcgagc tcgccgacgc cgatctcgac cacttccggc gcgtcatgcg acaggcccgc
    15421 tcggtggcga agacccgcgg cccggacgag gtgctggccg cagcggaagc gttgctcggc
    15481 cggatcaagg gaatcgagat tcccgagttc atccgggacc gcctggagcg gctgcgcatg
    15541 ctcatcgaca tgttgcgcga cgtcgagtgg aagctgccgg agccggaggc cacccgagta
    15601 ctcaacgccc tcgcctattt ttgcgaggcc gaggacctga tcccggacaa cgtaccgggg
    15661 ctgggctttc tcgacgacgc catcatggtc gagctggtgg tgcgtgagct gcgccacgaa
    15721 atcgaggcct accaggactt ctgttcctat cgtgccacgc gcgtcggcga gtccgccgat
    15781 gccgttacac gcgaagagtg gctgtcggcc cgccgcaagg ccctccagtc acggatgcgc
    15841 aaccgccgcc gcagggaccg ggagcgccgc cggggcggcg gcggcggccg atcgcccttt
    15901 tccctgttct gaccccgttg tcctgtacgc tggtcgcgct agcccggccg gccgcgacgg
    15961 ctcacttccc acgccaccca ggagctggct ggcgcgtcgg ggtcgagctc gacacgtgcg
    16021 cgcgccagcc ggcgaccgag catggcggcc gcggcacggg caccggaggt gaaccacttg
    16081 tcgggcagca gcaagcgcag tcgcctgcgc agcgcaccga tgtagggcgc cgtttcgtgc
    16141 atcgagtccc cgctctgcag cgaaccgcgt ttcatggcag gtagtagagc acgaccaggc
    16201 ccagcgccgc ccacatcccg gcctgcgg
//
LOCUS       DAOMPG010000026        16126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1571_length_16126_cov_4.319706, whole genome shotgun sequence.
ACCESSION   DAOMPG010000026 DAOMPG010000000
VERSION     DAOMPG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16126
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_16126_cov_4.319706"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLS34_02455"
     CDS             complement(<1..633)
                     /locus_tag="PLS34_02455"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRP86369.1"
                     /translation="MTRSLGELAVRYGLELRGDPDVRIARVGTLQQAGPDAVSFLANP
                     RYRKYLGSTRAGAVVLEEALAGQCAAAVLIANNPYAAYARIAADLHPAPAFAPGVHAA
                     ASVAADAEVADSACIGPGAVIESGAQVGPRASVGPNCVVGMGARIGPDSRLVANVTVC
                     HGVRLGARVLVHPGAVIGADGFGLAREPEGWIKVPQVGSVVIGDDVEIGAN"
     gene            complement(653..1174)
                     /locus_tag="PLS34_02460"
     CDS             complement(653..1174)
                     /locus_tag="PLS34_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRP86370.1"
                     /translation="MNAMTRISGALLLAALLAVPATAMAENKIGVVKLNRLFEEAPQS
                     LALQRSLTEEFAPRERDIRAKRDELMNVEKRIQEGEGFMSDDERRRLEQTLRDGQRDL
                     QRAQNEFVEDLNLRRNERLGDLQRLFISEIQAYGKAQGFDLILVDGIVHASDKLDITQ
                     QVLQVLQRRHQGG"
     gene            complement(1236..3623)
                     /gene="bamA"
                     /locus_tag="PLS34_02465"
     CDS             complement(1236..3623)
                     /gene="bamA"
                     /locus_tag="PLS34_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRP86371.1"
                     /translation="MKQFFLAGTAVLMVVVLGLGALPVAAQQGEPFVVRDMRVEGLKR
                     ISEGTVFNYLPVSLGDRLDSQRIAEAIRAVFGTGLFSSVEMRRDGDVLVIAVRERPSI
                     RNLTITGNKDIKTEDLMESLRGVGLMRGGTFDRPVLEDLQQFLTDQYYSRGKYSVSVD
                     TRVTEDEAENTVDISVRIKEGERARIRQVAIVGNEVFSDDEILDTFESRTRNWLSWYR
                     QDDRYSRETLVGDLEKLQSYYMDRGYANFEVRSTQVQISPDRQSIFITINVDEGEPYR
                     ISDIRLAGNMVVPEEQLRRLVLSKPGDTFSRRQLTQTAELINFRLGEEGFAFARVDPV
                     PTVSEDSNEVSVTFYIDPGKRAYVRRISFVGTTAINDEVLRREMRQLEGGYLSNRALE
                     RSEQRLQRLPFIEQVSKETTPVVGSDDLVDIEFNIKEGLPGSFGGGIGYSGLQGVILN
                     ANFVHTNFLGSGNRIEADVNTGRFSTIYSFLHTDPYVTPDGVARTVSASYRDITQFVS
                     GASEFGTKTGQLGLEYSYPISEYTRLRFGAQVLDTSLITNTASPVQSIRWVLLNGDAT
                     VTDVPGGSIYGTDFMAYELLLGWSYDSRNRVFFADRGSRHRVSLNYTVPGSDVEYYTL
                     RYDLLKYFPFFGPTYLEWTGEVMYGDAFGDTTELPPYKRFFGGGPGSVRGFREGYMGP
                     RDTNNRPFGGNLRFSSQLEMIVPVRGELGSTTRFSLFLDAGNIFSTDSTPFFDPRTGE
                     PSSFDFEASNMKYSVGASFQWLAPLGLFKFSYGFPLNDDSFDDTERFQFTIGSAF"
     gene            complement(3631..4988)
                     /gene="rseP"
                     /locus_tag="PLS34_02470"
                     /pseudo
     CDS             complement(3631..4988)
                     /gene="rseP"
                     /locus_tag="PLS34_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
     gene            complement(5042..6223)
                     /gene="ispC"
                     /locus_tag="PLS34_02475"
     CDS             complement(5042..6223)
                     /gene="ispC"
                     /locus_tag="PLS34_02475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRP86372.1"
                     /translation="MKGLAILGATGSVGVSTLDVVARNEGRFAVRALTGHRNWRLLLE
                     QCRRFRPAVAVLADAEAAASLGAAVREEGLPVEVQAGADALQQAARRDDVEFVMAAIV
                     GAAGLLPTLAAARAGKRVLLANKEAVVMAGELLTAALAASGGTLVPIDSEHNALFQCL
                     PEGRGADPNGAGVVSLVLTASGGPFLDTPLEALETVTPAAACRHPNWEMGRKISVDSA
                     TMMNKGLEIIEACRLFAVGPDRVRVVIHPESIVHSLVEYRDGSVLAQLGNPDMRTPIA
                     HALAWPERIASGVAPLDLAALGRLSFRPADPHRFPCLALAIAAAEAGGAAPIVLNAAN
                     EAAVAAFLDGRIRFTAIPAVIDAVLAQYAGPDPGELDAILALDAQARASAEEKIRELA
                     L"
     gene            complement(6220..7020)
                     /locus_tag="PLS34_02480"
     CDS             complement(6220..7020)
                     /locus_tag="PLS34_02480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRP86373.1"
                     /translation="MLKERIITALLLAAALLTVLFFAHADVGVVLFALLLLVAAWEWA
                     GLAQAPRAVHRAAFTLVIAVAIGMLAWRMPTGHLPPALIWVQLAVWAVAAAALARFPV
                     KIPRGVVIAAGLVILPLVWLTFAHVLKTRAFGASWALYLFVIVAAADVGAYFFGRTFG
                     RLKLAPRVSPGKTWEGLIGGLSLVALVGLGGAQYFGVSAAGLVPLVLAVAGISVVGDL
                     GISMLKRRIGLKDTGQLFPGHGGVLDRIDSLLAAIPLYTLGLHWLVAP"
     gene            complement(7014..7760)
                     /gene="uppS"
                     /locus_tag="PLS34_02485"
     CDS             complement(7014..7760)
                     /gene="uppS"
                     /locus_tag="PLS34_02485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRP86374.1"
                     /translation="MSVPDMPAHVAVIMDGNGRWARQRSRPRQAGHRAGVRTARAIVE
                     ACARRGIGVLTLFAFSSENWRRPRREVGALMRLFVEALDREVEELHGNGVRLVFIGDR
                     DGLSDTLQARMAAAEERTRGNDRLVLVIALAYGGRWDIVQATRRLAEDAAAGRIDPTA
                     IDEDALAGRMALAGLPDPDLLIRTGGEHRISNFLLWNLAYAECWFTDCLWPDFGEAEL
                     DAALARYRSVERRFGRTSEQLDPPPEGAPC"
     gene            complement(7787..8344)
                     /gene="frr"
                     /locus_tag="PLS34_02490"
     CDS             complement(7787..8344)
                     /gene="frr"
                     /locus_tag="PLS34_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRP86375.1"
                     /translation="MLQDIKKDAAERMGKSVESLRGELKKLRTGRAHTSLLDHITVEY
                     YGSEVPLSQVANVGIEDSRTLTITPWERSMVQVVEKAIMTSDLGLNPNTAGTVIRVPL
                     PPLTEERRRDMGKVVRHEGENAKVAIRNIRRDALHTVKELLKEKEITEDDDRRAHDEI
                     QQLTDKHIALVDKVVEEKEQELLEV"
     gene            complement(8359..9099)
                     /gene="pyrH"
                     /locus_tag="PLS34_02495"
     CDS             complement(8359..9099)
                     /gene="pyrH"
                     /locus_tag="PLS34_02495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRP86376.1"
                     /translation="MSRKVPKRILLKLSGEALLGGVDYGIDPEVISRIASELRDVREL
                     GIQVAVVIGGGNIFRGAGLAEGGMDRVTGDHMGMLGTVINALAMQDALEKLGVHSRVM
                     SAIKINEICEDYIRRRAIRHLEKSRVVLLAAGTGNPFFTTDTAASLRAIEINADLLLK
                     ATKVNGVYSADPKRFPDAQPYSRLTYDAVLDARLGVMDATAVVMCRDNDLPLRVFNVH
                     NKGDLLRIINGEDIGTLVTRAPDPAAEA"
     gene            complement(9238..10113)
                     /gene="tsf"
                     /locus_tag="PLS34_02500"
     CDS             complement(9238..10113)
                     /gene="tsf"
                     /locus_tag="PLS34_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018862309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRP86377.1"
                     /translation="MNISATLVKELRERTGAGMMECKRALVETGGDIEAAVEHMRKQG
                     LAKADKKAGRIAAEGLVVITVSADQKTAAVVEVNCETDFVAGGDVFRNFAGAVGELVL
                     EQRPADLDALLALPLAGATVEETRRGLVAKIGENIGVRRFEILSSDGLVGTYLHGTRI
                     GVLVAVKGGDEELARDLAMHIAASRPQYVDESGIPGDFLAKEREILVEQAKGEGKPAE
                     IVARMVEGRLRKQLAEITLVGQPFVKDPDTTVGKLLAANGATVQGFARFEVGEGIEKK
                     ADNFVAEVMAQVRGG"
     gene            complement(10185..11051)
                     /gene="rpsB"
                     /locus_tag="PLS34_02505"
     CDS             complement(10185..11051)
                     /gene="rpsB"
                     /locus_tag="PLS34_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRP86378.1"
                     /translation="MASISMRQMLEAGVHFGHQTRFWNPKMSPYIFGERNKIHIINLE
                     KTLPLYIKAQDFIRKLATDGGSVLFVGTKRSARESIQKEAERCGMPFVSYRWLGGMLT
                     NYKTVKQSIKRLFDLEAMAEDGTFESLSKKEVLGLKREMEKLERSLGGIKHMGSLPDA
                     LFIIDIEHERIAVAEANKLGIPIVAIVDTNCSPEGIDYVIPGNDDAMRAIQLYTAGVA
                     DAVLEGKASVPQVPAGEDDFVEVDEAAPRKPARKVDGGRARVAKPKPAAAPEAAEPAA
                     PEADAAGEATPE"
     gene            11284..12054
                     /gene="map"
                     /locus_tag="PLS34_02510"
     CDS             11284..12054
                     /gene="map"
                     /locus_tag="PLS34_02510"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRP86379.1"
                     /translation="MPVTIKSTAAEREGMRRAGRLAAQVLDMIGPHVQPGVSTDQLDA
                     ICHEYITGTLDAIPAPLNYRGFPKSICTSVNHVVCHGIPGERKLKKGDIVNIDITVIK
                     DGWHGDTSRMFYVGEPTVGGRRVSEVAHEAMRRGIEMVRPGVRLGDIGHVIQSYVEAE
                     NCSVVREYCGHGIGREFHEDPQVLHYGKPGTGMELQPGMTFTIEPMVNAGKRQVRLLP
                     DNWTVVTKDHSLSAQWEHTLLVTEDGHEVLTLGAEGHI"
     gene            11982..14732
                     /gene="glnD"
                     /locus_tag="PLS34_02515"
     CDS             11982..14732
                     /gene="glnD"
                     /locus_tag="PLS34_02515"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRP86380.1"
                     /translation="MGTHPAGHRGRPRSAHAGRRGPHLSAPGDKPAPALAEPGAPLPE
                     AGAQPGPDTIRRFRDALEAGDRELRQRFKADEPVEHLVRDRARLIDVALVRAWRMHLG
                     EAAAQLALVAVGGYGRGELHPCSDIDIMVVVPEGDGAAWRDPVSAFLTFLWDIGLEVG
                     HSVRSVADCAREAEADVTVVTTLMESRLLDGPTALFDAVQAVTGPERMWAPPAFFEAK
                     RSEQTARHHRYHDTAHNLEPNIKGSPGGLRDIQVVCWVAKRHFGTGSLHQLAQQGFLT
                     DYEYRRLLAGQSFLWRIRWALHTITGRREDRLLFDHQVKLAQLFGYEDASFTLAVEQF
                     MQKYYRTVQELARLNEMLLQLFEEAILLDPAAPPRPLNARFQVRNDFVEVTHDEVFER
                     TPSALLEIFLVLQQEPRLKGVSAGTIRLIRRALPLIDEEFRQNPRHHRLFMDIIRAPE
                     GVTHELRRMNRYGVLGRYIPAFGRVTGRMQFDLFHAYTVDAHTLFVVSNLRRFALSRF
                     DHEMPMCSRIMQSLPKQELAYLAGLFHDIAKGRGGDHSELGAVDAESFCLEHGLSPYD
                     ARLVAWLVRHHLVFSITAQKQDIHDPAVVAEFARRVGDEAHLDYLYVLTVADVRGTSP
                     KLWNAWKASLFEEFYDSVKRALRAGLESPIDSDQLIAETQEAALALLAARGLRAERAR
                     EVWRGFAGEYFLRHTPEEIAWHIRVLAGLEDRGEQSAVSVEQQTGRGGTAIMVCTLTG
                     QHSFARATGALDELGLNILDARIVPVGDGRNVHTYVVLESDGSPIDDHERLVQIEAAV
                     RKATLEHGGRMPTVLRQPPRQVRLFSTPTRIAFSEDHEGRRTVMELVAADRPGLLCEV
                     GKAFVEINASLRAAKIMTVGERAEDVFYITTLEGETLSAEQREQLARQLKTRLDQPAS
                     AA"
     gene            14762..15958
                     /gene="dapC"
                     /locus_tag="PLS34_02520"
     CDS             14762..15958
                     /gene="dapC"
                     /locus_tag="PLS34_02520"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRP86381.1"
                     /translation="MNPLLDRLYPYPFERLARLNAGATPPADRPHIALSIGEPRHAPP
                     AFVGAALTAALDRLAVYPKAAGLPELRAACAGWLERRFALGSGAVDPATMVIPVNGTR
                     EGLFSFVQAVLDPAAGGLVLMPNPFYQIYEGAALLAGATPHYLDTTAATGFLPDLDAV
                     PAETWRQCRILFICSPGNPTGAVMDDAYLLRVLELAARHDFVVASDECYADIYLDEDA
                     PPPGLLGACLRAGNTRFERCAVFHSLSKRSSVPGLRSGFVAGDPRIMDGFRLYRTYHG
                     CAVPEMVQLASVPAWSDDAHAAANRRLYQQKFDAVVPILAEVMPVSRPDAAFYLWLET
                     PGDDEAFTRGLYAAENVTVLPGRYLSRDHAGGNPGAGRVRVSLVAELDECIEAAHRIA
                     RFVRSL"
     gene            15989..>16126
                     /gene="dapD"
                     /locus_tag="PLS34_02525"
     CDS             15989..>16126
                     /gene="dapD"
                     /locus_tag="PLS34_02525"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928777.1"
                     /note="catalyzes the formation of
                     N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and
                     tetrahydrodipicolinate in the lysine biosynthetic pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRP86382.1"
                     /translation="MSNLQEIIEAAFEERQRFTPEDTPGDIRQAVAEALDALDDGRER
                     VA"
BASE COUNT         2451 a         5776 c         5420 g         2479 t
ORIGIN      
        1 attcgcgccg atctccacgt cgtcgccgat cacgaccgag ccgacctgcg gcaccttgat
       61 ccagccctcc ggctcgcgcg ccaggccgaa gccgtccgcg ccgattaccg cgcccggatg
      121 gaccagcacg cgcgccccga gccgcacccc gtggcacacc gtgacgttgg ccaccagccg
      181 gctgtccgga ccgatccggg cgcccatccc gaccacgcag ttcggcccca ccgaggcgcg
      241 cgggccgacc tgcgcgccgg actcgatcac ggcgccggga ccgatgcagg cgctgtcggc
      301 cacctcggcg tcggcggcga cggaggccgc agcatggacg cccggcgcga acgccggcgc
      361 cggatgcagg tcagcggcga tcctggcgta ggcggcgtag ggattgttcg cgatcagcac
      421 cgccgcggcg cattgcccgg ccagcgcctc ctcgagcacc actgcgccgg cccgcgtgct
      481 gccgaggtat ttgcggtagc gcgggttggc caggaacgag acggcgtccg ggccggcctg
      541 ctgcagcgtg ccgacccggg caatgcgcac atcgggatcg ccccgcagct cgagcccgta
      601 acggacggcg agctcgccga gactcctggt catcgcaccg cggccgccgg cctcacccgc
      661 cctggtgccg gcgttgcagc acctgcagga cctgctgggt gatgtccagc ttgtcgctgg
      721 catggacgat gccgtccacc aggatcaggt cgaagccctg cgccttgccg taggcctgga
      781 tctcgctgat gaacagccgc tgcaggtcgc cgaggcgctc gttgcggcgc aggttcaggt
      841 cctcgacgaa ctcgttctgc gcccgctgca ggtcgcgctg gccgtcgcgc agggtctgct
      901 cgaggcgccg gcgctcgtcg tcggacatga agccctcgcc ttcctggatg cgcttctcga
      961 cgttcatcag ctcatcgcgc ttggcgcgga tgtcgcgctc gcgcggcgcg aattcctcgg
     1021 tcagcgagcg ctgcagcgcc agcgactgcg gcgcctcctc gaagagccga ttcagcttga
     1081 cgacgccgat cttgttttcc gccatcgccg tggcgggaac tgcgagcagt gcggcgagca
     1141 gcagcgcgcc ggagattcgg gtcattgcgt tcatgcgtac gctgtcctca atgattttgt
     1201 gtcgtattgg cctggccggc gcccggttcg ggacatcaga acgccgagcc gatggtgaac
     1261 tggaagcgct ccgtatcgtc gaaactgtcg tcgttcagcg ggaagccgta gctgaacttg
     1321 aagaggccga gcggcgcgag ccactggaac gatgcgccga ccgagtactt catgttggaa
     1381 gcctcgaagt cgaagctgct cggctcgccc gtccgcggat cgaagaacgg cgtgctgtcg
     1441 gtggagaata tattaccggc gtccaggaac aaactgaaac gcgtggtgct gccgagttcg
     1501 ccgcgcaccg ggacgatcat ttccagctgg ctgctgaagc gcaggttgcc gccgaaggga
     1561 cggttgttgg tgtcccgcgg tcccatgtag ccctcgcgga agccgcgcac cgagcccggg
     1621 ccgccgccga agaaacgctt gtagggcggc agctcggtgg tgtcgccgaa ggcgtccccg
     1681 tacatcacct cgccggtcca ctcgaggtag gtggggccga agaaagggaa atacttgagc
     1741 aggtcgtagc gcagggtgta gtactccaca tcgctgcccg gcaccgtgta gttgagcgac
     1801 acgcggtgcc gcgacccgcg atccgcgaag aacacccggt tcctcgagtc gtaggaccat
     1861 cccagcagca gctcgtaggc catgaaatcc gtgccgtaga tggagccgcc cggcacgtcg
     1921 gtgaccgtcg cgtcgccgtt gagcagcacc caccggatgg actgcacagg gctggccgtg
     1981 ttggtgatca ggctggtgtc gagcacctgc gcgccgaacc tgagacgcgt gtactcggag
     2041 atcggataac tgtattccag cccgagctgg ccggtcttgg tgccgaactc cgacgcgccc
     2101 gagacgaact gggtgatgtc ccggtaggag gcggacaccg tgcgcgccac gccgtccggc
     2161 gtgacgtagg gatcggtgtg caggaaactg tagatcgtgc tgaaccggcc ggtgttgacg
     2221 tcggcctcga tgcgattgcc gctgccgaga aaattggtgt gcacgaagtt ggcgttcagg
     2281 atgacgccct gcaggcccga gtagccgatg ccgccgccga agctgcccgg caggccctcc
     2341 ttgatgttga actcgatgtc caccaggtcg tcgctgccga ccacgggggt ggtctccttg
     2401 ctgacctgct cgatgaacgg cagtcgctgc aggcgctgct cggagcgctc cagcgcccgg
     2461 ttggacaaat agccgccctc cagctggcgc atttcccgcc gcagcacctc gtcgttgatg
     2521 gcggtggtgc cgacgaagct gatgcgcctg acgtaggctc gcttgcccgg atcgatgtag
     2581 aaggtcaccg agacttcgtt gctgtcctcc gacacggtcg gcaccgggtc cacgcgtgcg
     2641 aacgcgaaac cttcttcgcc gagccggaaa ttgatcagtt cggcggtctg ggtcagctgg
     2701 cggcgcgaga aggtgtcgcc gggcttgctc agcaccagcc gcctgagctg ctcctccggt
     2761 accaccatgt tgccggccag ccggatgtcc gagatcctgt acggctcgcc ctcgtcgacg
     2821 ttgatggtga tgaagatgct ctgccggtcg ggcgagatct gtacctgcgt cgagcggacc
     2881 tcgaagttgg catagccgcg atccatgtaa tacgactgca gcttctcgag atcgccgacc
     2941 agcgtctcgc gcgagtagcg gtcgtcctgc cggtaccacg acagccagtt gcgcgtgcgg
     3001 ctttcgaaag tgtccaggat ctcgtcgtcg ctgaacactt cgttgccgac gatcgccacc
     3061 tggcggatgc gggcgcgctc gccttccttg atccgcaccg agatgtcgac ggtgttctcg
     3121 gcctcgtcct cggtgacccg cgtgtccacc gacacgctgt acttgccgcg gctgtagtac
     3181 tggtcggtca ggaattgctg caggtcctcg agcaccggcc ggtcgaaggt accgccgcgc
     3241 atcaggccca cgccgcgcag cgactccatc aggtcctcgg tcttgatgtc cttgttgccg
     3301 gtaatggtga ggttgcggat cgagggccgt tcgcgcacgg cgatcaccag cacgtcgccg
     3361 tcgcggcgca tctcgacgct cgagaacagg ccggtgccga acacggcgcg aatcgcctcc
     3421 gcgatgcgct ggctgtcgag ccggtcgccg aggctgaccg gcaggtaatt gaacacggtg
     3481 ccctcggaaa tgcgcttcag cccctcgacc cgcatgtcgc gcaccacgaa gggctcgccc
     3541 tgctgcgccg cgaccgggag cgcgccgagg ccgaggacga ccaccatgag aacggccgtc
     3601 ccggccagga aaaactgctt cactcgcacc tcagccactg gtcagccgga cgatatcgtt
     3661 gaaaaaggcg aagctcatca agagcagcag cagcaggatg ccgacctgct ggccgatgat
     3721 ctgcacccgc tcggataccg ggctgccttt cgcgatctca acgaactggt agacgatctg
     3781 cccgccgtcc aacaggggaa tcggcagcag gttgatgatg cccaggctga tactgacgat
     3841 cgccaggaag ctcaggaagg cgatcacccc ggccgcggcc gtgaagcccg ccgcctgggc
     3901 gatgttgatc ggcccgctga tgttcttcac cgacacctgg ccgacgatca tgcggcccat
     3961 catggtgacc gtcagcgccg acatccgcca ggtctccgcc agcgcctcgc cgaccgcgcc
     4021 gagcggcccg aaacgctgct cggtgcgcgc cgggtcccac atctcgggcg tcaccagcgg
     4081 cgccgcgccg atccgcccga cccgctgccc gtcctcgtcg gccgcgccga tgaacagctc
     4141 gagcgattgc gcctggccgt cgcgctccac caccagccgg atggtttcgc cgggccgctc
     4201 acgcaccagc gccacccagt ccgcccagtc ggccaccggg cgcccgtccg cgctgagcac
     4261 gcgatcaccc gcggcgcggc cgcggtgccc tcgggcagca cttcaccgat cacggcggga
     4321 aattccggcc gccagaggcc gatgccgagg ccggggaaca tcgcgcccgg ctccgtcagg
     4381 gcatgggtgt cgccctggta ctcgatgcgc gcctggcggc ggctgccgtc ctcgccctcg
     4441 acctcgaggg tggcgcgccc gcccggcacc atggcgcgca gcagctcgag ctgcgcgtcc
     4501 atcatggtgg cggtgcgctc gccgtccacc gcgagaatcc ggtcgccggc cctgaggccg
     4561 gccacggccg ccggcgactc ggcttcgacc gtgccgacca gcggcttcat cgccggcacg
     4621 ccggccacga acatgaccca gtaggcggcg atggcgaaca ggaagttgaa agccgggccc
     4681 gcgaacaaca ccgcgatgcg gcgcgacggc ggctggctgt tgaagcagcg cggcaggtcc
     4741 tcgggcggca cgtcgccctc gcgctcgtcg agcatcttca cgtagccgcc cagcgggatg
     4801 gcggacaccc agtactcggt gccgtccgcg ccggtgcggc gccacaaggg gcggccgaag
     4861 ccgatcgaga agcgcagcac cttgatgccc aggcggcgcg ccacccagaa atggccgaac
     4921 tcgtgcaccg ccacgagcac ggcgatggcc acgacgaacg ccagggctga tgtcaggact
     4981 ccgctcaatt actgcctctg ttcacgatga cgggccggcg gcggtacagg cgccggactc
     5041 attagagtgc caattcccgg attttctcct cggccgacgc ccgggcctgc gcatccagcg
     5101 ccaggatcgc gtcgagctcg cccggatcgg ggcccgcgta ttgtgccaga actgcgtcga
     5161 tcactgccgg aatcgcggtg aaccggatcc ggccgtcgag gaatgccgcg accgccgcct
     5221 cgttggcggc gttcagcacg ataggcgccg cgccgccggc ctccgccgcg gcgatcgcca
     5281 gcgccaggca gggaaagcgg tgcgggtcgg ccggccggaa actcagccgg ccgagcgccg
     5341 cgaggtccag cggcgccacg ccggaagcga tgcgctccgg ccaggccagc gcgtgggcga
     5401 tcggcgtacg catgtcggga ttgccgagct gcgccagcac cgagccgtcg cggtactcca
     5461 ccagcgagtg gacgatgctc tcgggatgga tcaccacccg gacccggtcc ggcccgaccg
     5521 caaacaagcg gcaggcctcg atgatctcga gccccttgtt catcatggtg gccgagtcca
     5581 ccgagatctt gcggcccatc tcccagttgg ggtgccggca ggccgccgcc ggggtcaccg
     5641 tctcgagcgc ctccagcggc gtgtccagga aggggccgcc cgaggcggtc agcaccaggc
     5701 tcaccacgcc cgcgccgttc gggtccgcgc cgcgcccctc cggcaggcac tggaacagcg
     5761 cgttgtgctc gctgtcgatg ggcaccaggg tgccgccgga cgccgccagc gcggcggtca
     5821 acagctcccc ggccatcacc accgcttcct tgttggccag cagcacacgc ttgccggccc
     5881 gcgccgccgc cagcgtcggc agcagccccg ccgcaccgac gatggccgcc atcacgaact
     5941 cgacgtcgtc gcgccgtgcc gcctgctgca gtgcatccgc gccggcctgg acctcgacgg
     6001 gcaggccctc ctcgcgcacc gccgccccga ggctggccgc ggcctcggca tcggccagca
     6061 ccgccaccgc cgggcggaag cgccggcact gctcgagcaa cagccgccag ttgcggtggc
     6121 cggtcagcgc gcgcaccgcg aagcgcccct cgttgcgcgc caccacgtcc agcgtgctga
     6181 cgccgacgct gccggtcgcg ccgaggatgg ccagtccctt cacggggcga ccagccagtg
     6241 aaggccgagg gtatagagcg gaatggccgc cagcaggctg tcgatccggt cgagcacgcc
     6301 gccgtggccg ggaaagagct ggccggtgtc cttcaggccg atgcgccgct tgagcatgct
     6361 gatgcccagg tcgcccacca ccgagatgcc ggcgaccgcc agcaccagcg gcaccaggcc
     6421 ggcggcggac acaccgaaat actgcgcgcc gccgagcccc accagcgcga ccagcgacag
     6481 cccgccgatc agcccttccc aggtcttgcc ggggctgacc cgcggcgcca gcttgaggcg
     6541 gccgaaggtg cgcccgaaaa agtacgcccc gacgtccgcg gccgccacga tcacgaacag
     6601 gtagagcgcc caggaggcgc cgaaggcgcg tgtcttgagc acgtgcgcga aggtcagcca
     6661 gaccagcggc aggatcacga ggccggcggc gatgaccacg ccgcgcggga tcttcaccgg
     6721 gaagcgcgcc agcgcggcgg ccgccaccgc ccagaccgcc agctgcaccc agatcagcgc
     6781 cggcggcagg tggccggtgg gcatgcgcca ggcgagcata ccgatggcga cggcgatcac
     6841 cagcgtgaag gcggcgcggt gtaccgcgcg cggcgcctgc gccagccccg cccattccca
     6901 ggccgccacc agcagcagca gcgcgaacag caccacgccg acatcggcgt gcgcgaagaa
     6961 cagcaccgtg agcagcgcag ccgccagcag caaggccgtg atgatccgtt ccttcagcac
     7021 ggggcgccct ccggcggcgg gtcgagctgc tccgaagtgc gcccgaagcg gcgctcgacg
     7081 ctgcggtagc gcgccagcgc cgcgtccagt tcggcttcgc cgaaatccgg ccacaggcag
     7141 tcggtgaacc agcactccgc ataggccagg ttccagagca ggaagttgct gatgcggtgc
     7201 tcgccgccgg tgcggatcaa aaggtcggga tccggcaggc cggccagcgc catgcgcccg
     7261 gccagcgcgt cctcgtcgat cgccgtcggg tcgatccgcc cggcggcggc gtcctcggcc
     7321 agccgccgcg tcgcctggac aatatcccag cgcccgccgt aggccagcgc gatcaccagc
     7381 accaggcggt cgttcccgcg ggtgcgctct tcggcggccg ccatgcgcgc ctgcagcgta
     7441 tccgacagtc cgtcacggtc gccgatgaac accagccgga cgccgttgcc gtgcagctcc
     7501 tcgacctcgc ggtcgagcgc ctcgacgaac agccgcatca acgcgccgac ctcgcgccgt
     7561 ggccggcgcc agttctcgct ggaaaaagca aacagcgtca gcacgccgat gccgcgccgc
     7621 gcgcaggcct cgacgatcgc ccgcgccgtc ctgacgccgg cgcgatggcc ggcctggcgg
     7681 gggcgggacc gctggcgggc ccaacggccg ttgccgtcca tgatgacggc gacgtgggca
     7741 ggcatgtcgg gaacgctcat gggatttgcg gcgaggcccg gcggcctcac acctcgagca
     7801 gttcctgttc cttctcctcg acgaccttgt ccaccagcgc aatgtgcttg tcggtcagct
     7861 gctggatctc gtcgtgcgcg cggcgatcgt cgtcctcggt aatctccttc tccttgagga
     7921 gctccttgac ggtgtgcagc gcgtcccggc ggatgttgcg gatggccacc ttggcgttct
     7981 cgccctcgtg gcgcaccacc ttgcccatgt cgcggcgccg ctcctcggtg agcggcggca
     8041 gcggcacgcg gatcaccgtg ccggcggtgt tgggattgag gccgaggtcg gaggtcatga
     8101 tggccttctc gaccacctgc accatgctgc gctcccacgg cgtgatggtg agcgtgcgcg
     8161 agtcctcgat gccgacgttg gcgacctggc tcaacggcac ctcgctgccg taatactcga
     8221 ccgtgatgtg atcgagcagg ctggtgtggg cgcgccccgt acgcagcttc ttgagctcgc
     8281 cgcgcagcga ttcgacgctc ttgcccatgc gctcggcggc gtctttcttg atgtcctgca
     8341 acatgctcgt cctccccctc aggcttccgc ggccgggtcc ggcgcgcggg tcaccagcgt
     8401 gccgatgtcc tcgccgttga tgatcctcaa caggtcgccc ttgttgtgca cgttgaacac
     8461 gcgcagcggc aagtcgttgt cgcggcacat caccaccgcc gtcgcgtcca tcacgccgag
     8521 gcgcgcgtcg agcacggcgt cgtaggtcag gcggctgtag ggctgcgcgt ccggaaagcg
     8581 cttcgggtcg gccgagtaga cgccgttcac cttggtggcc ttcagcagca ggtcggcgtt
     8641 gatctcgatg gcccgcaggc tggccgcggt gtcggtggtg aagaagggat tgccggtacc
     8701 ggcggcgagc agcaccacgc ggctcttctc gaggtggcgg atggcgcgcc ggcggatgta
     8761 gtcctcgcag atctcgttga tcttgatcgc ggacatgacg cgcgaatgga cgccgagctt
     8821 ctccagcgcg tcctgcatgg ccagcgcgtt gatcaccgtg ccgagcatgc ccatgtggtc
     8881 gccggtgacc cggtccatgc cgccctcggc gagccccgcg ccgcggaaga tgttgccccc
     8941 gccgatgacc accgccacct ggatgccgag ttcgcggacg tcacgcagct cgctggcgat
     9001 gcggctgatg acctccgggt cgatgccgta gtcgacgccc ccgaggagcg cctcgccgct
     9061 cagcttgagg aggattcgtt tcggaacttt ccggctcatc tcgccccctg cgatcgattc
     9121 gtcggccttg cctgccgcaa aaaagaaccc cgctgtccgc ggggccctca agcttacctc
     9181 atctgccggc gccgtggccc atggcggtgg aggattcgac gcgcggcacc gcggccctca
     9241 gccgccgcgc acctgcgcca tcacttccgc gacgaagttg tcggccttct tctcgatgcc
     9301 ctcgccgact tcgaagcgcg cgaaaccctg cacggtggcg ccgttggccg caagcagctt
     9361 gccgacggtg gtgtcgggat ccttgacgaa gggctggccg accagcgtga tctcggcgag
     9421 ctgcttgcgc agccggccct cgaccatccg ggcgacgatc tcggcgggct tgccctcgcc
     9481 cttggcctgc tccaccagga tctcgcgctc cttggcgagg aagtcgcccg ggatgccgct
     9541 ctcgtccacg tactgcggcc gcgaggccgc gatgtgcatc gccaggtcgc gcgccagttc
     9601 ctcgtcgccg cccttgaccg ccaccagcac gccgatgcgg gtgccgtgca ggtaggtgcc
     9661 caccaggccg tccgacgaga ggatctcgaa gcggcgcacg ccgatgttct cgccgatctt
     9721 ggccaccagg ccgcggcgag tctcctcgac ggtcgcgccg gccagcggca gcgccagcag
     9781 ggcgtccagg tcggccgggc gctgctcgag cacgagctcg cccacggcac cggcgaagtt
     9841 gcggaagacg tcgccgccgg cgacgaaatc cgtctcgcag ttcacttcga cgaccgcggc
     9901 ggtcttctga tcggccgaca cggtgatgac caccaggccc tcggccgcga tccggccggc
     9961 cttcttgtcg gccttggcca gcccctgctt gcgcatgtgc tcgacggcgg cctcgatgtc
    10021 gccgccggtc tccaccagcg cgcgcttgca ttccatcatg ccggcgccgg tgcgctcgcg
    10081 tagttccttg accagggttg cggaaatatt catgaatctc ttacctcgaa acggggacac
    10141 gccttatctc gaaataagga atgtcccctt gcagtccggc gtggttactc gggagtggct
    10201 tccccggcgg cgtccgcttc gggcgcagcc ggctcggcag cctcgggcgc agccgcgggc
    10261 ttcggcttcg ccacacgggc acggccaccg tcgaccttgc gcgcgggctt gcgcggcgcg
    10321 gcctcgtcca cttccacgaa gtcgtcctcg ccggccggca cctgcggcac ggaggccttg
    10381 ccctccagca cggcgtcggc caccccggcg gtgtacagct ggatggcgcg catcgcgtca
    10441 tcgttgcccg ggatgacgta gtcgatgccc tcgggcgagc agttggtgtc cacgatggcg
    10501 acgatcggga tgccgagctt gttggcctcg gcgacggcga tgcgctcgtg ctcgatgtcg
    10561 atgatgaaca gcgcgtcggg caacgagccc atgtgcttga tgccgcccag gctgcgctcg
    10621 agcttctcca tctcgcgctt caggccgagc acctctttct tggagaggct ctcgaaggtg
    10681 ccgtcctcgg ccatcgcctc gaggtcgaac agtcgcttga tggactgctt gacggtcttg
    10741 tagttggtca gcatgccgcc cagccagcgg tagctgacga agggcatgcc gcaacgctcc
    10801 gcttccttct ggatggactc gcgcgccgag cgcttggtgc cgacgaacag cacgctgccg
    10861 ccgtcggtgg ccagcttgcg gatgaagtcc tgcgccttga tgtacagcgg cagggtcttc
    10921 tcgagattga tgatgtggat cttgttgcgt tcgccgaaga tgtagggtga catcttcgga
    10981 ttccagaaac gggtctggtg gccgaaatga acgccggcct ccagcatctg gcgcatggag
    11041 atagaagcca tgttgtactc ctcgggttag gcctccacgc accccacccg gcaacccgct
    11101 tttccatgga aaagaaggca cccagccgag cgtgacggtg cgtgtgtggg atttaattgc
    11161 gattaaggcg cgcgctttat accataggcg gccttgctga acaatctccc cggccacccc
    11221 ggggcccacc ctaagtggtt gaaccgggat gaagttttca cggctcggga ggccgacatc
    11281 gatatgcccg tgaccatcaa gagcaccgca gccgagcggg agggcatgcg ccgggccggg
    11341 cggctggcgg cgcaggtgct cgacatgatc ggcccgcacg tccagccggg cgtgtccacc
    11401 gaccagctcg acgccatctg ccacgagtac atcaccggca cgctggacgc gatcccggcg
    11461 ccgctcaact accgtggctt ccccaagtcc atctgcacct cggtcaacca cgtcgtctgc
    11521 cacggcatcc cgggcgagcg caagctcaag aagggcgaca tcgtcaacat cgacatcacc
    11581 gtcatcaagg acggctggca cggcgacacc agccgcatgt tctacgtcgg cgagcccacc
    11641 gtcggcggtc ggcgggtcag cgaggtggcg cacgaggcca tgcggcgcgg catcgagatg
    11701 gtccggcccg gcgtgcgcct cggcgacatc ggccacgtca tccagagcta cgtcgaggcc
    11761 gagaactgct cggtggtgcg cgagtactgc ggccacggca tcggccggga gtttcacgag
    11821 gacccgcagg tgctgcacta cggcaagccg ggcacgggca tggagctgca gccgggcatg
    11881 actttcacca tcgagcccat ggtgaacgcc ggcaagcgcc aggtgcggct gctgccggac
    11941 aactggacgg tggtcaccaa ggatcactcc ctgtccgccc agtgggaaca caccctgctg
    12001 gtcaccgagg acggccacga agtgctcacg ctgggcgccg agggccacat ctgagcgccc
    12061 cgggcgacaa gccggcgccc gccctcgccg agccgggcgc gccgctgccg gaggccggcg
    12121 cccagccggg cccggacacc atccggcgat tccgcgatgc gctcgaggcc ggcgaccgcg
    12181 agctccggca gcgtttcaag gccgacgagc cggtcgagca cctggtgcgc gaccgcgccc
    12241 ggctgatcga cgtcgcgctg gtgcgcgcct ggcgcatgca cctcggcgag gcggcggcgc
    12301 aactcgcgct ggtcgcggtc ggcggctacg gccgcggcga gctgcatccc tgctccgaca
    12361 tcgacatcat ggtggtcgtc cccgagggtg acggcgccgc ctggcgcgac ccagtgtccg
    12421 ccttcctgac cttcctctgg gacatcggcc tggaggtcgg ccacagcgtg cgctcggtgg
    12481 cggactgcgc gcgcgaggcc gaggccgacg tcaccgtcgt caccacgctg atggagtcgc
    12541 gcctgctgga cggcccgacg gcgctgttcg acgccgtgca ggcggtcacc ggcccggagc
    12601 ggatgtgggc cccgccggcc ttcttcgaag ccaagcgcag cgaacagacc gcgcgtcacc
    12661 accgctacca cgacaccgcc cacaacctcg agccgaacat caagggcagc cccggcggac
    12721 tgcgcgacat ccaggtggtc tgctgggtgg ccaagcgcca cttcggcacc ggcagcttgc
    12781 accagctcgc gcagcagggc ttcctcaccg actacgagta ccgccgcctg ctggccgggc
    12841 agagcttcct gtggcggatc cgctgggcgc tgcacaccat caccggccgt cgcgaggacc
    12901 ggttgctgtt cgaccaccag gtcaagctgg cgcagctgtt cggctacgag gacgccagct
    12961 tcacgctggc cgtcgagcag ttcatgcaga agtactaccg caccgtgcag gagctggcgc
    13021 gcctcaacga gatgctgctg cagctgttcg aggaagcgat cctgctcgac cccgccgcgc
    13081 cgccgcggcc gctgaacgcc cgcttccagg tgcgcaacga tttcgtcgag gtgacccacg
    13141 acgaggtgtt cgaacgcacg ccctcggcgc tgctggagat cttcctggtg ctgcagcagg
    13201 agccgcgact caagggcgtc agtgccggca ccatccgcct gatccgccgc gcgctgccgt
    13261 tgatcgacga ggagttccgc cagaacccgc gccatcaccg cctgttcatg gacatcatcc
    13321 gcgcccccga gggcgtcacc cacgagctgc gccgcatgaa ccgctacggc gtgctcggcc
    13381 gctacatccc ggccttcggg cgggtcaccg ggcgcatgca gttcgacctg ttccacgcct
    13441 acaccgtgga tgcgcacacc ctgttcgtgg tgtcgaacct gcggcgcttc gcgctgtcgc
    13501 gcttcgatca cgagatgccg atgtgcagtc gcatcatgca gtcgctgccc aagcaggagc
    13561 tggcctacct cgccggcctg ttccacgaca tcgccaaggg ccgcggcggc gaccactcgg
    13621 agctcggcgc ggtggacgcc gagtcattct gcctcgagca cggcctgtcg ccgtacgacg
    13681 cgcggctggt cgcctggctg gtgcgccacc acctggtgtt ctccatcacc gcccagaagc
    13741 aggacatcca cgacccggcg gtggtcgccg agttcgcccg ccgggtcggc gacgaggcgc
    13801 acctcgatta cctctacgtg ctgaccgtgg ccgacgtgcg cggcaccagc ccgaagctgt
    13861 ggaacgcctg gaaggcgtca ctgttcgagg agttctacga cagcgtcaag cgcgcgctgc
    13921 gcgccgggct ggagtcgccg atcgactccg accagctgat cgccgagacc caggaggccg
    13981 cgctggcgct gctcgccgcg cgcggcctgc gcgccgagcg ggcgcgcgag gtgtggcgcg
    14041 gcttcgccgg cgagtacttc ctgcgccaca cgccggagga gatcgcctgg cacatccgcg
    14101 tgctggccgg gctggaggac cgcggcgagc agtcggcggt gtcggtcgaa cagcagaccg
    14161 gccgcggcgg caccgccatc atggtctgca ccctgaccgg ccagcacagc tttgcgcgcg
    14221 ccaccggcgc actggacgag ctcggcctga acattctcga cgcacgcatc gtgccggtcg
    14281 gcgacggccg caacgtgcac acctacgtgg tgctggagag cgacggctcg cccatcgacg
    14341 accacgagcg gctggtgcag atcgaggccg cggtgcgcaa ggcgaccctc gagcacggcg
    14401 gccgcatgcc gaccgtgctg cgccagccgc cgcgccaggt gcggctgttc tcgacgccca
    14461 cgcgcatcgc cttctccgag gatcacgagg gccggcgcac cgtcatggag ctggtggccg
    14521 cggaccgtcc cgggctgctg tgcgaggtgg gcaaggcctt cgtcgagatc aacgcctcgc
    14581 tgcgcgcagc caagatcatg accgtcggcg aacgcgccga ggacgtgttc tacatcacca
    14641 ccctggaggg cgagacgctg agcgccgagc agcgcgagca actcgcccgg caattgaaga
    14701 cgcgactcga ccagccggcc tccgccgcct gagccaacga cccgccaagg cagccctgcg
    14761 catgaatccc ctgctcgacc ggctctaccc ctaccccttc gagcggctcg cccggctgaa
    14821 tgccggcgcg accccgccgg ccgaccggcc gcacatcgcg ctgtccatcg gcgagccgcg
    14881 ccatgcgccg ccggccttcg tgggcgccgc gctgaccgcg gcgctcgacc ggctggcggt
    14941 ctatccgaag gccgccggcc tgccggagct gcgtgcggcc tgcgcgggct ggctggaacg
    15001 gcgcttcgcg ctgggctccg gcgcggtgga cccggcgacc atggtcatcc cggtaaacgg
    15061 cacgcgcgag gggctgttct ccttcgtaca ggcggtgctc gacccggccg cgggcggcct
    15121 ggtgctgatg ccgaatccct tctaccagat ctacgagggc gcggcgctgc tggccggcgc
    15181 gacgccgcat tacctcgaca ccaccgccgc caccggcttc ctgccggacc tcgacgccgt
    15241 gccggccgag acctggcggc aatgccgcat cctgttcatc tgcagcccgg gcaacccgac
    15301 cggcgcggtg atggacgatg cttacctgct gcgggtgctg gagctcgctg cgcgccatga
    15361 cttcgtggtg gcctcggacg agtgctacgc cgacatctat ctcgacgagg acgcgccgcc
    15421 gcccgggctg ctcggcgcct gcctgcgcgc cggcaacacc cgcttcgagc gctgcgccgt
    15481 gtttcacagc ctgtcgaagc gttccagcgt gccggggctg cgttccggct tcgtcgccgg
    15541 cgatccgcgc atcatggacg gcttccgcct gtaccggacc taccacggct gcgccgtgcc
    15601 cgagatggtg cagctcgcca gcgtgccggc ctggtccgac gacgcgcacg cggcggccaa
    15661 ccggcgcctg taccagcaga aattcgacgc cgtggtgccg atcctggccg aggtcatgcc
    15721 ggtgagccgg ccggacgccg ccttttacct ctggctggag acgcccggcg acgacgaagc
    15781 cttcacgcgt ggcctgtacg cggccgaaaa cgtcaccgtg ctgcccggcc gctacctgtc
    15841 gcgcgaccat gccggcggca accccggcgc gggccgcgtg cgcgtctcgc tggtcgcgga
    15901 actggacgaa tgcatcgagg cggcgcatcg tattgcccgg ttcgtccgtt cgctctgacg
    15961 ccgccatgtc atccacgggg ccacgcacat gagcaacctg caggaaatca tcgaggccgc
    16021 gttcgaggaa cgccagcgct tcacgccgga ggacacgccg ggcgacatcc gccaggcggt
    16081 ggctgaggca ctcgacgcgc tggacgatgg ccgcgagcgc gtcgcc
//
LOCUS       DAOMPG010000027        15998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1583_length_15998_cov_4.513705, whole genome shotgun sequence.
ACCESSION   DAOMPG010000027 DAOMPG010000000
VERSION     DAOMPG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15998
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_15998_cov_4.513705"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLS34_02530"
     CDS             <1..577
                     /locus_tag="PLS34_02530"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRP86383.1"
                     /translation="DFPGEYGSGTLPRGGVDIHRTRIHMVSDSSFPKAWLAAGLCAAF
                     ALAACGRSQDPPAGAASSPPPAATQPADAEPSRPAPGGQPRAAATVTITDADDGRAVA
                     LQRGQVVELRLKANRTAGFTWIPVQNVLPVMNTDGVPLYESEGDDTAGTEIGRFIALE
                     PGHAHLVFEYRRPFEPGAPPQKSITLHFDVE"
     gene            574..1605
                     /locus_tag="PLS34_02535"
     CDS             574..1605
                     /locus_tag="PLS34_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRP86384.1"
                     /translation="MTAGRPATRITEPRAAPPADPAVPLTLLTGFLGAGKTTLVNRAL
                     NGDFGGRIGVLVNDFGALNIDQALIADRTDGIVSLENGCICCSLRGDLVAATMRLLDR
                     PDAPQRVLLEASGVADPAPIAGTFLAPAFRRRLRLDGAVCVVDAEQALEAPELEALQL
                     RQIGFSDLVIINKTDLAGPQRVRRLAERIRREFQGARLLEAVRCAVPLEVLFSTPAGT
                     ARAAGPAATHDEAPSVSFDSWSYESARAFSADALHDLARRLPACVYRAKGIVRLRGSG
                     RRDDRRGILQIVGRRADLVAAGPWRDRERSSRLVFIGAAGELEPGRIRALVTDCLSKD
                     QPEMPTCGD"
     gene            1593..1940
                     /locus_tag="PLS34_02540"
     CDS             1593..1940
                     /locus_tag="PLS34_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86385.1"
                     /translation="MWRLIAPIIALLLLGASFLRAGDTALVATCGLLLLLLFVPRPWA
                     ARIVQLALVLAALRWLWLTWLLAGARAAAGVPYTRMVAILGTVALLTLLATLVFRAVR
                     LRRYYRLDRAAAP"
     gene            1955..2395
                     /locus_tag="PLS34_02545"
     CDS             1955..2395
                     /locus_tag="PLS34_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRP86386.1"
                     /translation="MTAPRPPKHRLHEPQTYLDHAARLMLIAAGLICVGLGALGILLP
                     GLPTTPFLLLAAYCFARSSEHFHNWLLNHRWFGSYVRNFESGRGMTRPAKATTLLVMW
                     LSFGFTIVLFVPVVWGQVSMFLLAVTVSVYIMRLPTPAPEATTD"
     gene            complement(2347..4305)
                     /locus_tag="PLS34_02550"
     CDS             complement(2347..4305)
                     /locus_tag="PLS34_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRP86387.1"
                     /translation="MIAQGLLAAALGTTLLLAEPDAAGLTDEVLAKVDAAALALEIGA
                     PANVRLPDGTALELIVTARRRHPSGNVTVLARPTDPAQSGYLVTLTGRPETGLAGTVL
                     APQGEYRLEAREGALQPPRMVRVVPPANAWLDDVAWHARPASAPAVTFDEDILSDEHT
                     TPLNVLVLYTAGARDGRNDAEWTAFLDDLFAVTNDAYAHSGAQVEFNRVGERLVAASE
                     TAGSFELLSQVTPRSGSFDAAVFGAVEDWRLAADAHLVALLRRWQPSQSNCGAAGLQA
                     CGDDAQCYNANLGYSVTSLGDCTALTLAHELGHNLGSAHEPGLDWGGTYPYAHAHVTP
                     GGVGTVMSRTGTTRTAQFSSPAADCDGYPCGIEEVSDNVRSLRQARHYIARWISDRSI
                     TLWDFAPAQVTRGGSLALNFLWFGVLTPRFHIDLHRDGAAVARLADTESLPEGFKLLA
                     IPASLPLGTGYSLRVTAADLPAIYTESGQFELVPPAGPPGVIAMGAAGVTAPQGASFV
                     ELTVHRTGGTAGLAVVEFETVDGTARAGTHYTATTGSFAWPDGADGAYNITVPITTAA
                     AQGQDRTFSVVLRNPSAGTTLGTPAATVVTIRAPAGGGGGGGGGGSPGPWSLLLAAAL
                     LACPAGARAGRRGQSVVASGAGVGSRMM"
     gene            complement(4379..4942)
                     /locus_tag="PLS34_02555"
     CDS             complement(4379..4942)
                     /locus_tag="PLS34_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRP86388.1"
                     /translation="MNARVSRLCVAAVACVAAATAVADAPDRAAEGRAMAGQFGAELR
                     TALQDAMAAGGPIAAIKVCNEDAPRIAAAAAQASGATVGRTSLKLRNPDNRPDEHERA
                     VLEEFAAAVAANPGAPPPERLDTLADGRVRFMSAIIVQPPCLACHGDALAPPVAAAIG
                     ALYPDDQARGYQAGELRGAFTITWPAD"
     gene            complement(4954..5820)
                     /locus_tag="PLS34_02560"
     CDS             complement(4954..5820)
                     /locus_tag="PLS34_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP86389.1"
                     /translation="MAKAPEVRGLYHQPTSTFSYVAWDPGSRRAAIVDAVLDYDGAAG
                     RTGTSAAAALLAIVAAEGLEVQWILETHAHADHLSAANYLHQRTGAPVGTGAGVTAVQ
                     ATFSEIYNLGPDFATDGRQFDRLFVDGETFMLGDLQVEVLATPGHTSDSITFLVGDAA
                     FVGDTLFSPDYGSARCDFPGGDAATLFRSVQRLYRLPPATRLFLCHDYPPGGREPRCE
                     WSVAEQREGNIHIGADTRMEDFVTMREARDQTLSMPALIIPSIQVNIRAGRLPEPDNN
                     GTRYLRVPLDTL"
     gene            5988..6278
                     /locus_tag="PLS34_02565"
     CDS             5988..6278
                     /locus_tag="PLS34_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRP86390.1"
                     /translation="MRAHAHDAARLLKALSNSSRLMILCSLAKGELTVGQINERVPLS
                     QSALSQHLAVLRRDGLVRTRRSAQTIYYRLAHGPAGRIIDTLYEVFCGQTSA"
     gene            6453..7001
                     /locus_tag="PLS34_02570"
     CDS             6453..7001
                     /locus_tag="PLS34_02570"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRP86391.1"
                     /translation="MNFRSEFFGVMLAGGLLACTATLATALSPPLDDLVEVGRGDMRW
                     FGIDLYEARLLNRDGRYQFGNDAGPLALEITYRRNISRERLVSTTEREWRRLAAELGL
                     RNDGRVASWLEALAGIWPDVAPGDRILALREAAGPTRFYGNDGLLGIIEDPDFGPAFL
                     GIWLHPETRAGDLRAALIGTSP"
     gene            6998..7537
                     /locus_tag="PLS34_02575"
     CDS             6998..7537
                     /locus_tag="PLS34_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HRP86392.1"
                     /translation="MKRTTLAMVAALLLAGCTASVRDYEGREPRLDLQAYFDGPLVAW
                     GIVQDRSGEASRSFRVDMVGRWDGDTGVLEEDFTWSDGATERRVWTFRKIDEHTYSGT
                     AGDVVGEARGEAFGNALRWRYTLALPWNDGTINVALDDWMWLVQDDVLINRSEIRKFG
                     LRVGEVTIFFQKAESGHES"
     gene            7527..8417
                     /locus_tag="PLS34_02580"
     CDS             7527..8417
                     /locus_tag="PLS34_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRP86393.1"
                     /translation="MNRDVSRELAANGMSEHEAADAFSGGSVGALFPIRVVARLTGIN
                     PVTIRAWERRYRLVQPERTPGGHRLYSRADVELLRAASRLVDQGVSISRATRLLDDPA
                     PRRESRNDRLLARFLERLAALDDDGMSAVFEASLARRSSGGFAALLDELPAVAPSLPR
                     LEQQFLDAWLEGLLAFRTYQRMAQAGERRVLVCSPAEPARRTWALVFALGLVGSGLRP
                     ALLAGPGRDDLGEAARRTACVAIVLGGTGADWADVQPAELPAPVFLEDARDEFLPLGG
                     DLDDARRRVMDFLQAGTGRA"
     gene            8414..8863
                     /locus_tag="PLS34_02585"
     CDS             8414..8863
                     /locus_tag="PLS34_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRP86394.1"
                     /translation="MSAAMLADGGAPSRLVARFQQTFQELRHDNLSRLAAVYADDVLF
                     EDPLHRVEGLAALTEYFERMYAGVESISFEFGEVIDAPGQAMLTWTMRMTHRRLRPGE
                     PLALPGASHIRYDQRVHYHRDYFDAGALLYERLPLLGTVVRAVRRRV"
     gene            8887..10164
                     /locus_tag="PLS34_02590"
     CDS             8887..10164
                     /locus_tag="PLS34_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP86395.1"
                     /translation="MRIAIIGSGISGLTAARRLCPDHDVTVFEANHYIGGHTNTVDVD
                     LDGRRWAVDTGFIVFNDRTYPNFISLMNEIGVESQPSDMGFSVRCDRSGLEYCGSSLN
                     QLFAQRRNLVRPGFWRMLRDILRFNREARELLEGREDTLELGAYLAREGYSRQFTEQY
                     IVPMGAAIWSTDPQTMLRFPARYFVDFFNNHGLLSVNDRPQWRVIRGGSRSYIGPLTA
                     PFADRIQLNAPVMRVHRNAAGVELVLRDGHRAWFDGVVFACHSDQALAMLAQPTEAER
                     SILGAIPYQPNRAVLHTDERLLPRRRAAWAAWNYHIPAERRSAVSVTYNMNILQGLDA
                     ERQFLVTLNPASEIAADKVVREIVYQHPVYTPEGVAAQRRRAELMGANRSFYCGAWWS
                     YGFHEDGVKSGLVAAAAVQAWSQDAKLSLRRAS"
     gene            10133..10921
                     /locus_tag="PLS34_02595"
     CDS             10133..10921
                     /locus_tag="PLS34_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRP86396.1"
                     /translation="MQSCLYAGRVSHRRHGGGDDPVSNRFEYRLFMVWLDLAELDTVF
                     RGRWLWSTRRRNLAWLRREDHFGDPSQPLDTAVRDLVLQRTGQRPAGPIRMLTHLRYF
                     GHCFNPVTFYYCYDRAGEHTETIVAEVSNTPWGERHCYVLPRSAARLRGPSQEFALDK
                     QFHVSPFLPMETRYQWRFTEPDARLLVHLGNEQQGRHAFDATLTLERRDITRASLAAA
                     LLRFPFMTLRVVLLIYWQALKLKLKGARFYTHPAKRDAGMETPS"
     gene            10948..12201
                     /locus_tag="PLS34_02600"
     CDS             10948..12201
                     /locus_tag="PLS34_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRP86397.1"
                     /translation="MAPDSAAGRITPTRLQRFARRQLVSRMARLEHGALEVHDGEGML
                     RFGKDREGTGLRARIEVLHPQFWADAVFGGTTGAGEAYIHGLWKCDDLTALVRMMVAN
                     RHVLEEVDGAATRFAALGRRLGHWLNRNSRAGSRRNISAHYDLGNDLFALFLDPTMNY
                     SSGIFETPDATMEQASIAKMDAACRKLDLQPGDHLLEIGTGWGGLAIHAAGRYGCRVT
                     TTTISREQHALAAQRIRDAGLEDRIELLLCDYRDLEGRYDKLVSIEMIEAVGHQYLDT
                     YFRTCSSLLKPDGLMLLQAITIRDEHYEQALKSVDFIQRFVFPGGFLPSVSAMSASLA
                     RTGDLQMLHLQDIGLHYATTLKRWRERFFANLDAVRALGYPDSFIRLWEFYLCYCEGA
                     FLERNIGNVQLLLAKPRNRRASLGY"
     gene            complement(12198..13154)
                     /locus_tag="PLS34_02605"
     CDS             complement(12198..13154)
                     /locus_tag="PLS34_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRP86398.1"
                     /translation="MNESKLAPVLVVMDPADGAGHALARARRVAAALGAPLELFACEF
                     DQAMEGSHFRVTERLEAAREARVVARREWLESVAAPLSADGLEVRTAAEYANPRHEAV
                     ARRALAVGAAYVVMRTHYHEWLARATLSAADWQLIRICPAPLLLVKGAAWPATPRLLA
                     AVDPSHRDDRHARLDHAILDEAECLGEALGGEAWVAHCMLSMASLESTAVLGMMPEAL
                     GSSPEEYAGDLLQRLRAAVLRLLEGRGIPAARLRIAEGRPERELPRLVDDLGIDLLVA
                     GGISRSRLEQVFIGGTAERLLDRVGCDLLVVKPPGFGCPVEL"
     gene            complement(13160..13930)
                     /locus_tag="PLS34_02610"
     CDS             complement(13160..13930)
                     /locus_tag="PLS34_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP86399.1"
                     /translation="MPAARDSNVVSIAQLRQSCRSCSLRDLCLPLGLGADDMQKLESI
                     VHSRGPIRSGEHLFREGDAFHALYAVKSGAVKTYTIDNQGREHVLGFHIAGEVAGLDG
                     IHSGRNRCNAVALQATTVCAVPFARLEQVIHEVPGLQAQMLRIMSRELSASAQLATDH
                     SAEERLAGFLLSLSKRYARRGLPPDLLSLPMPRRDLASHLRLATETISRLFARFQEDG
                     LVSVRRREVQILDAEALEALGGSFLPDGDGQRRSPQLR"
     gene            14112..14792
                     /locus_tag="PLS34_02615"
     CDS             14112..14792
                     /locus_tag="PLS34_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HRP86400.1"
                     /translation="MRTFASALALAAALGLAAPAYGHEAGDWLVRFGASNVDPKSDNG
                     SIDLTATGILGVQDVTVDSKVGVTFNVAYMYTDTIGIEVLAALPFKHDVNVAGLPEPA
                     ASVTHLPPTVSLQYYFLPGSNFRPYAGIGLNLTWFMDEDKKAGLAAADTALGVTTDIK
                     VDSTSVGLAAQLGFDYKINQQWFLNVDVRYIDISTDATLTVNGANFATAKVDVDPLVY
                     GIHLGYRF"
     gene            14981..>15998
                     /gene="ccoN"
                     /locus_tag="PLS34_02620"
     CDS             14981..>15998
                     /gene="ccoN"
                     /locus_tag="PLS34_02620"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRP86401.1"
                     /translation="MTTASAYNDKVVRQFTIMTIVWGVVGMLVGVIIAAQLLWPALNF
                     DISWLSYGRLRPLHTNAVIFAFGGCALFASSYYVVQRTCHVRLFGGGLAAFTFWGWQA
                     VIVLAAVTLPLGLTQGKEYAELIWPIDILIAAVWVSYAVVFFGTIAVRKVKHIYVANW
                     FFGAFILTVAVLHIINSLALPTSMLHSYPVYSGVVDAMVQWWYGHNAVGFFLTAGFLG
                     MMYYFVPKQAGRPIYSYRLSVVHFWALISLYMWAGPHHLHYTTLPDWAQSLGMVFSLI
                     LLAPSWGGMINGIMTLSGAWHKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNALS
                     HYTDW"
BASE COUNT         2461 a         5621 c         5335 g         2581 t
ORIGIN      
        1 ggattttccc ggagaatacg ggtccgggac gctgccgcga ggcggcgtcg acatccatcg
       61 aacgaggatt cacatggtgt ctgacagcag tttcccgaag gcctggcttg ccgccggcct
      121 gtgcgccgct ttcgccctgg cggcctgcgg ccggtcgcag gacccgcccg ccggggccgc
      181 atcgagccca ccgcccgcgg cgacgcaacc ggccgatgcc gagccgtccc gccccgcacc
      241 tggcggacag ccgcgtgcag ccgcgaccgt gaccatcacc gatgccgatg acgggcgtgc
      301 ggtggcgctg cagcgcggcc aggtggtgga actgcgactc aaggccaacc gcaccgccgg
      361 cttcacctgg atcccggtgc agaacgtcct gccggtgatg aacaccgacg gcgtgccctt
      421 gtacgagagc gagggggacg ataccgccgg aacggaaatc gggcgtttca tcgcgttgga
      481 gcccggacac gctcacctgg tgttcgagta ccggcgcccc ttcgagccgg gtgcaccgcc
      541 gcagaaatcc atcacgctcc acttcgacgt cgagtgacgg caggccggcc cgccacccgc
      601 atcaccgagc cccgggcggc cccgccggcc gacccggcgg tgccgctcac cttgctgacc
      661 ggattcctgg gcgccggcaa gaccacgctg gtgaatcgcg cgctcaacgg cgatttcggc
      721 ggccggatcg gcgtgctggt caacgatttc ggcgcgctga acatcgatca agcgctgatc
      781 gccgaccgga ccgacggtat cgtgagcctg gaaaacggct gcatctgctg ttcgctgcgc
      841 ggtgacctgg tcgccgccac gatgcgcctg ctggatcgcc cggatgcccc gcaacgcgtc
      901 ctgctggagg cgagcggcgt ggcggatccg gcgcccatcg ccggcacttt cctggcgccg
      961 gccttccgcc gcaggttgcg gctcgacggc gcggtttgcg tggtcgatgc agagcaggcg
     1021 ctcgaggcac ccgaactgga agccctgcag ctgcgccaga tcggcttctc ggacctggtg
     1081 atcatcaaca agaccgacct ggccggcccc caacgggtgc gtcgcctggc ggagcggatc
     1141 cgccgcgagt tccagggggc caggctcctg gaggcggtgc gctgcgcagt tcccctcgaa
     1201 gtgctgtttt ccactcctgc cgggacggca agggctgcgg ggcctgcggc cacgcacgat
     1261 gaggcgccgt ccgtgtcgtt cgactcctgg tcgtacgaat ccgcacgggc attttccgcc
     1321 gatgcgctgc acgacctggc gcggagactg ccggcctgcg tgtaccgcgc caagggcatc
     1381 gtgcgcctcc gcggcagcgg acgccgggac gaccggcgcg gcatcctgca aatcgtcggc
     1441 cggcgtgccg acctggtcgc cgccggaccg tggcgcgacc gggagcgcag ctcgcggctg
     1501 gtcttcatcg gcgctgccgg tgagctggag cccgggcgca tccgcgcgct ggtgacggac
     1561 tgcctcagta aagaccaacc ggagatgccc acatgtggcg actgatcgcg ccgataatcg
     1621 ctttgctgct actgggcgcc agcttcctgc gcgccggcga cacggcgctg gtcgccacct
     1681 gcggcctgct gctgctgcta ctgttcgtgc cccgcccatg ggctgcgcgg atcgtccagc
     1741 tggcgttggt gctggcggcg ctccgctggt tgtggctgac ctggctgctg gccggtgcgc
     1801 gcgccgccgc cggcgtgcct tatacacgca tggtggcgat cctcggcacg gtcgcgctgc
     1861 tgaccctgct cgccacgctg gtgttccggg cggtgcggct gcgccgctat taccggctgg
     1921 accgggccgc ggcgccctga gcacccgcat cgcgatgact gccccgcgcc cgccgaaaca
     1981 tcgactccac gagccgcaga cctacctgga tcatgcggcg cggctgatgc tgatcgcagc
     2041 cgggctgatc tgcgtcgggc tcggggcgct cggcattctg ctgcccggac tgccgaccac
     2101 accgttcctg ctgctggccg cgtactgctt tgcgcgcagc tccgagcact tccacaactg
     2161 gctgctcaac catcgctggt tcggcagcta tgtgcgcaat ttcgagtccg ggcgcggcat
     2221 gacgcgcccc gccaaggcca ccaccctgct ggtgatgtgg ctgtcgttcg gcttcaccat
     2281 cgtgctgttc gtgccggtgg tctgggggca ggtctcgatg ttcctgctcg ccgtgaccgt
     2341 gtcggtctac atcatgcggc tgccgacgcc ggcgccggag gcgaccacgg actgaccgcg
     2401 tcgcccggcc cgggcgcccg ccggacaggc cagcagcgcg gccgccagca acagcgacca
     2461 cggacccggg gatccgcctc cgccgcctcc gccgccacca ccggccggtg cgcggatggt
     2521 cacgacggtg gctgcgggcg tgccgagcgt cgtgcccgcg gatgggttgc gcagcacgac
     2581 cgaaaacgtc cggtcctgcc cctgcgcggc ggcggtggtg atgggcacgg tgatgttgta
     2641 ggcgccgtcg gcgccgtccg gccacgcgaa cgagcccgtg gtcgccgtgt agtgcgtccc
     2701 ggcccgcgcc gtaccgtcca cggtttcgaa ttcgacgacc gccaggcccg cggtgccgcc
     2761 cgtgcggtgc accgtgagct cgacgaagct cgccccctgc ggcgcagtca ctccggcagc
     2821 gcccatggcg atgacgccgg gcggcccggc cggcggcaca agctcgaact ggccggactc
     2881 ggtatagatg gcgggcaaat ctgcggccgt cacgcgcagc gagtagccgg tcccgagcgg
     2941 cagtgacgcg gggatcgcga gcagcttgaa gccctcgggc agggactcgg tgtccgccag
     3001 cctggcgacc gcggcgccgt cacgatgcag gtcgatgtgg aagcgcgggg tcagcacgcc
     3061 gaaccacagg aagttcagcg ccagggagcc gccgcgtgtc acctgcgcag gggcgaaatc
     3121 ccacagcgtg atgctgcgat cgctgatcca gcgcgcaatg tagtggcgcg cctggcgcag
     3181 cgagcggaca ttgtcgctga cttcctcgat gccacagggg tagccgtcgc aatccgcagc
     3241 aggcgacgag aactgcgccg tccgcgtggt cccggtccgc gacatcacgg tgcccacgcc
     3301 gccaggcgtc acatgcgcat gcgcgtaggg ataggtgccg ccccagtcaa ggccgggctc
     3361 gtgcgcggat cccaggttgt ggccgagttc atgggccagg gtgagtgccg tgcaatcgcc
     3421 gagtgacgtc acggagtagc cgaggttggc gttgtagcat tgcgcgtcgt ccccgcaggc
     3481 ctgcaggccc gccgcaccgc aattggattg cgacggctgc cagcgccgca acagcgcgac
     3541 gaggtgcgca tcggcggcga gccgccagtc ctccactgcg ccaaacacgg ctgcgtcgaa
     3601 agaaccgctg cgcggcgtca cctgtgacaa caactcgaaa ctgccggccg tctcgctggc
     3661 cgccaccagt cgttccccga cacggttgaa ctcgacctgg gcgccgctgt gcgcataggc
     3721 gtcattggtc acggcgaaca ggtcgtcaag gaacgcggtc cactcggcat cattgcgccc
     3781 gtcgcgcgcg ccggccgtgt acagcaccaa tacgttgagc ggcgtcgtgt gctcgtccga
     3841 caggatgtcc tcgtcgaagg tcacagccgg cgcggatgcc gggcgggcgt gccaggcgac
     3901 gtcgtcgagc caggcgttcg caggcggcac gacgcgcacc atgcgcggcg gctgcagcgc
     3961 tccttcgcgc gcctccagcc ggtattctcc ctgtggagca agcaccgtac cggccagccc
     4021 cgtttccggc cggccggtca gcgtcaccag gtagcccgac tgcgcgggat ccgtggggcg
     4081 cgccagcacg gtgacgttgc cgctggggtg ccggcgacgt gccgtgacga tcaactcgag
     4141 cgcggtcccg tccggcagcc ggacgttggc cggcgccccg atttccagcg caagggccgc
     4201 cgcgtcaacc ttcgcgagga cttcatcggt caggcccgca gcatcgggtt ccgccagcag
     4261 cagggtcgtc ccgagcgccg cggccagcag gccttgggca atcatgggga aattatgccg
     4321 cagcggccgg cgacgtggga gccgcgccga gggctgcgcc cggccgactg ccggagcgct
     4381 agtccgccgg ccaggtgatg gtgaacgcgc cgcgcaattc gccggcctgg tagccgcgcg
     4441 cctggtcgtc cggatacagc gccccgatcg ccgcggcgac cggcggcgcg agtgcatcgc
     4501 cgtgacaggc caggcagggc ggctggacga tgatggcgct catgaagcgc acgcgcccgt
     4561 ccgccagcgt gtcgaggcgc tccggcgggg gcgcgccggg attcgccgcc actgctgcgg
     4621 caaactcctc gagcaccgcc cgctcatgct catccggccg gttgtcggga ttgcgaagct
     4681 tgagcgaggt acggccgacc gtcgcaccgg acgcctgcgc cgcggccgcc gcgatgcgcg
     4741 gcgcatcctc gttgcagacc ttgattgccg cgatcgggcc gcctgcagcc atggcgtcct
     4801 gcagggccgt gcgcaactcg gcaccgaact ggccggccat ggcgcgcccc tcggccgcgc
     4861 gatccggtgc gtcggccaca gcggtcgcgg ccgccacgca ggcaactgcg gcgacacaga
     4921 ggcgtgatac tcgtgcgttc atcgggattt tccttacagc gtgtcgagcg gcacgcgcag
     4981 gtagcgggtg ccgttgttgt cgggctcggg caggcgtcct gcccggatgt tcacctggat
     5041 ggagggaatg atcagcgccg gcatgctgag cgtctggtcg cgcgcctcgc gcatggtcac
     5101 gaaatcttcc atgcgggtgt cggcgccgat gtggatgttg ccctcacgct gctcggcaac
     5161 gctccattcg caacgcggct cccggccacc cggaggatag tcgtggcaga ggaacaaccg
     5221 ggtcgccggg ggcaatcggt acaggcgctg caccgagcga aacagcgtcg ccgcatcccc
     5281 gcccggaaaa tcgcagcgtg ccgaaccgta gtcgggcgag aacagcgtgt cgcccacgaa
     5341 agcggcgtcg ccgaccagga aggtgatgct gtcgctggtg tgcccaggcg tggccagcac
     5401 ttcgacctgc aggtcgccca gcatgaaagt ctccccgtcc acgaacagcc ggtcgaactg
     5461 ccggccgtcg gtggcgaaat ccgggcccag gttgtagatc tcgctgaacg tcgcttgcac
     5521 ggccgtgacc cctgcgccgg tccccaccgg cgcgccggtg cgttggtgca ggtagttggc
     5581 ggccgacagg tggtcggcat gcgcatgggt ctccaggatc cactgtacct cgagcccctc
     5641 cgcggcaacg atcgccagca gcgcggcggc ggcgctcgtg ccggtgcgcc cggcggcccc
     5701 gtcgtagtcc aggacggcgt cgacaattgc cgcccggcgg ctgccgggat cccacgcgac
     5761 gtaggaaaag gttgaggtcg gctggtgata cagccccctg acttccggcg ccttggccat
     5821 gctgctatct ccggggcgac tcaggcaccc attatttctt attatcctaa tttagtaaat
     5881 ttctctttta tggttgcttc ggctagcata gcggcatgaa cagtcaaccc gccaagctga
     5941 cgccgccgga gctgtgcggg ccggatctcg cggtcgatcc ggaacagatg cgcgcgcacg
     6001 cgcacgacgc cgcgcggctc ctcaaggccc tgtcgaacag ctcaagactg atgatcctct
     6061 gcagcctggc caagggcgaa ctgacggtcg gccagatcaa cgagcgcgta cccttgtccc
     6121 agtcggccct gtcgcaacac ctcgcagttc tgcgccgcga cgggctggtg cgcacgcgcc
     6181 gctcggcgca gaccatctac taccgcctgg cccatgggcc ggccggacgc atcatcgaca
     6241 ccctctacga ggtcttctgc ggccagacct ccgcctgacc tccgcccctc ccgaaacgac
     6301 gcctgactga atccggcgga ccgcccgccg gcgaactccc ctgtactgct gccctgcgcc
     6361 tgtcgaaccg gtgtacaggc cggggccgct gaatccatcg cggccggcgc agcgatgtac
     6421 agcttgtggc aacttttggg gagatctggc ggatgaactt ccggagcgag tttttcggcg
     6481 tgatgctggc cggcggcctg ctggcctgca cggccaccct ggcgacggcg ctttcgccac
     6541 cgctggacga cctggtcgaa gtcggccggg gcgacatgcg ctggttcggc atcgacttgt
     6601 acgaggcgcg gctgctcaac cgcgacggcc gctaccagtt tggcaacgac gctggtccgc
     6661 tggcgctcga gatcacctat cgccggaata tctcccgcga gcgcctggtc agcactaccg
     6721 agcgcgagtg gcggcggctg gcagccgaac tcggcctgcg gaacgacggc cgggtcgcca
     6781 gctggctcga ggcgctggcc ggaatctggc ccgacgtcgc acccggcgat cgcattctcg
     6841 ccctgcggga ggcggctgga ccgacgcgct tctacggcaa cgacggactg ctcggcatca
     6901 tcgaggaccc cgacttcggc ccggcctttc tcggcatctg gctgcatccg gagacccgtg
     6961 ccggcgacct gcgggccgcg ctgatcggta cctcgccatg aagcgcacca cactcgcaat
     7021 ggtcgccgcc ctgctgctgg ccggctgcac cgcttcggtg cgtgattacg aaggacgcga
     7081 gccgcgcctc gacctgcaag cctacttcga cgggccgctg gtcgcatggg gcattgtcca
     7141 ggatcgctcc ggcgaggctt cgcgcagctt ccgggtcgac atggtggggc gctgggacgg
     7201 cgacaccggc gtcctcgagg aggatttcac gtggagcgac ggcgccaccg agcgccgcgt
     7261 gtggacgttc cgcaagatcg acgagcacac ctattcaggc accgccggtg acgtggtcgg
     7321 cgaagcgcgc ggcgaggctt tcggcaacgc cctgcgctgg cgctacacgc tggccctgcc
     7381 ctggaacgac ggcaccatca acgtggcgct cgatgactgg atgtggctgg tccaggacga
     7441 cgtgctgatc aaccgctcgg agatccgcaa gttcgggctc cgcgtcggcg aggtcacgat
     7501 cttcttccag aaagcggagt ccggtcatga atcgtgatgt ttcccgcgaa ctcgccgcca
     7561 acgggatgag cgagcacgag gctgccgacg ccttcagcgg cggcagcgta ggggcgttgt
     7621 tccccatccg cgtggtggcg cgcctgaccg gcatcaaccc cgtgacgatt cgcgcctggg
     7681 agcgacgcta tcgcctggta cagccggagc gcacgccggg cgggcaccgc ttgtacagtc
     7741 gggccgacgt ggagcttctg cgcgccgcct cgaggctggt cgaccagggc gtgtcgatca
     7801 gccgggcgac ccgattgctg gacgatccgg cgccgcggcg ggaatcgcgc aacgaccggc
     7861 tgctggcgcg ctttctcgag cggctcgcgg ccttggacga cgacggcatg agcgcggtct
     7921 tcgaggcctc gctggcgcgg cgctcgagcg gcgggttcgc agccctgctg gacgaactgc
     7981 ccgccgtcgc gccgtccctg ccgcgcctgg agcagcagtt cctcgacgcc tggctggagg
     8041 gactgctggc cttccgcact taccagcgca tggcgcaggc cggcgagcgg cgcgtgctgg
     8101 tgtgcagccc ggcagagccg gcccgccgga cctgggcgct ggtgttcgcg ctgggcctgg
     8161 tcggttccgg gctgcgtccc gccctgctcg cgggtccggg gcgcgacgat ctcggcgaag
     8221 cggcacgtcg tacggcctgc gtcgcgatcg tactcggcgg caccggcgcc gactgggccg
     8281 acgtccagcc cgcggaattg ccggcgccgg tgtttctcga ggacgcccgc gacgagttcc
     8341 tgccacttgg cggcgacctg gacgacgccc ggcgccgcgt catggacttt ctccaggccg
     8401 ggacgggtcg ggcatgagtg cggcgatgct tgcggacggc ggggccccgt cgcgcctcgt
     8461 ggcgcggttc cagcagacct tccaggagtt gcgccacgac aacctgtcgc gcctcgccgc
     8521 agtctacgcg gacgacgtgc ttttcgagga tccgctgcac cgtgtcgagg ggcttgccgc
     8581 gctcactgag tatttcgagc gcatgtacgc cggcgtcgag tcgatcagct tcgagttcgg
     8641 cgaggtcatc gacgcgccgg gacaggcgat gctcacctgg accatgcgca tgacgcaccg
     8701 gcggctgcgt cccggcgagc cactcgcact gccgggcgcc agccatatcc gctacgacca
     8761 gcgggtgcac taccaccgcg actacttcga cgcgggggcc ctgctgtacg aacgcctgcc
     8821 gctgctcggc accgtggtgc gcgccgtgcg ccgccgcgtc tgacccctta ttcacaggac
     8881 aagcacatga gaatcgccat tatcggcagc ggcatctccg gcctgaccgc ggcccgcagg
     8941 ctctgcccgg accacgacgt cacggttttc gaagccaacc actacatcgg cggccacacc
     9001 aacaccgtcg acgtcgatct cgacggccgg cggtgggccg tcgacaccgg cttcatcgtg
     9061 ttcaacgacc ggacctaccc gaacttcatc agcctgatga acgagatcgg cgtcgaatcg
     9121 cagcccagcg acatgggttt cagcgtgcgc tgcgaccgca gcgggctgga gtactgcggc
     9181 agctcgctca accagctgtt cgcccagcgc cgcaacctgg tccgccccgg cttctggcgc
     9241 atgctgcggg acatcctgcg cttcaaccgc gaggcccgcg agctgctcga aggccgcgag
     9301 gacacactcg agctcggcgc ctacctggcc cgcgagggct attcacggca gttcaccgag
     9361 caatatatcg tgccgatggg cgcggcgatc tggtccaccg acccgcagac catgctcaga
     9421 ttcccggcgc gctacttcgt ggacttcttc aacaaccacg gcctcttgtc ggtgaacgat
     9481 cgcccgcagt ggcgcgtcat tcgtggcggc agccgcagct atatcggtcc gctgaccgca
     9541 ccgtttgcgg accggatcca gctcaatgcg ccggtgatgc gtgtacatcg caacgccgca
     9601 ggcgtcgagc tggtgctgcg cgacggccac cgcgcatggt tcgacggcgt ggtgttcgcc
     9661 tgccattcgg accaggccct ggccatgctc gcgcaaccga ccgaggccga gcgcagcatt
     9721 ctgggcgcca tcccctacca gccgaaccgt gccgtgctgc acaccgacga gcgcctgctg
     9781 ccgcgtcgtc gggcggcatg ggcagcctgg aactaccaca tcccggcgga gcgccggtcc
     9841 gccgtgtcgg tcacctacaa catgaacatc ctgcagggcc tggacgccga gcggcagttc
     9901 ctggtgacgc tgaacccggc cagcgagatc gcggccgaca aggtcgtcag ggaaatcgtg
     9961 taccagcacc cggtctacac ccccgaaggc gtcgctgcgc agcggcggcg tgccgagttg
    10021 atgggcgcca accgcagctt ttattgcggc gcctggtgga gctacggctt ccacgaagac
    10081 ggggtcaaga gtggactggt cgccgcagcg gccgtgcagg cctggagcca agatgcaaag
    10141 ctgtctctac gccgggcgag ttagccaccg ccggcatggc ggcggcgacg atccggtctc
    10201 gaaccgtttc gagtaccggc tgttcatggt gtggctcgac ctggccgagc tggacaccgt
    10261 gttccgtggc cgctggctct ggtcgacccg gcgcaggaac ctggcctggc tgcgccgcga
    10321 ggaccatttc ggcgatccgt cacagccgct ggacaccgcg gttcgcgatc tcgtgctgca
    10381 acgcaccggt caacgtccgg ccgggccgat ccgcatgctc acgcacctgc gctacttcgg
    10441 ccactgcttc aacccggtga ctttttacta ttgttacgac cgggccggcg aacacaccga
    10501 gacaatcgtg gcggaagtga gcaacacgcc ctggggcgag cggcactgct acgtgctgcc
    10561 acgcagtgcg gcccggttgc gtgggcccag ccaggaattc gcgctggaca agcaattcca
    10621 tgtctcgccg ttcctgccca tggaaacccg ttaccagtgg cgcttcaccg aacccgatgc
    10681 ccgcctgctg gtgcatctgg gcaacgaaca gcagggacgg catgccttcg acgccacgct
    10741 gaccctggag cggcgtgaca ttacccgcgc gtcgctcgcc gccgcgctgc tgcgattccc
    10801 gttcatgacc ctgagggtcg tgctgctgat ctactggcag gcgctcaagt tgaagctcaa
    10861 gggcgcccgc ttctacacgc atccggcgaa acgcgatgcc ggcatggaga ccccgtcatg
    10921 atgagagagc acatcattcc cgccggcatg gcaccggact cggccgccgg ccgcatcacg
    10981 ccgacccgct tgcaacgctt cgctcgccgg caactggtca gtcgcatggc gcggctggag
    11041 cacggcgccc tcgaggtgca cgacggcgaa ggcatgctgc gcttcggcaa ggatcgcgaa
    11101 ggcaccgggc tgcgcgcacg catcgaggtg ctgcacccgc agttctgggc cgacgcggtg
    11161 ttcggcggca cgaccggcgc cggcgaggcc tatatccacg ggctgtggaa atgcgatgac
    11221 ctgacggccc tggtgcgcat gatggtggcc aataggcacg tgctcgagga ggtggacgga
    11281 gccgccacgc gcttcgcggc gctcggccgg cgtctcggcc actggctcaa ccgcaacagc
    11341 cgcgccggca gccgccgcaa catctcggcg cactacgacc tcggcaacga tttgtttgcc
    11401 ctgttcctcg acccgaccat gaactattcc agcggtattt tcgaaacgcc ggacgccacc
    11461 atggaacagg cctcgatcgc caagatggac gctgcatgcc ggaagctcga cctgcaaccc
    11521 ggcgatcacc tgctggagat cggcaccggc tggggcggcc tcgcgattca cgccgccggc
    11581 cgctacggct gccgcgtcac gaccaccacg atttcgagag agcagcatgc gctggccgca
    11641 caacgcattc gcgacgcggg gctggaagac cgcatcgaac tgctgttgtg cgactaccgc
    11701 gacctcgaag gccgatacga caagctggtg tccatcgaga tgatcgaggc ggtgggccac
    11761 cagtacctcg atacctattt ccgcacctgc tcgagcctgc tgaagcccga cggcctgatg
    11821 ctgttgcagg cgatcaccat ccgcgacgag cattacgaac aagcgctcaa gtcggtggac
    11881 ttcatccagc gcttcgtatt tcccggtggc ttcctgcctt cggtgtccgc gatgagtgcc
    11941 agcctggcgc gcaccggcga cctgcagatg cttcatttgc aggacatcgg gctgcactac
    12001 gccaccaccc tgaagcgctg gcgtgagcgc tttttcgcaa acctcgacgc ggtgcgcgcg
    12061 ctcggctacc cggacagctt catccggcta tgggagttct atctctgcta ctgcgagggg
    12121 gcgttcctcg agcgcaacat cggcaatgtg cagctgttgc tggcgaaacc ccgcaaccgg
    12181 cgcgcctcgc tcggctacta gagctccacc gggcagccga accccggcgg cttgaccacc
    12241 agcaggtcgc agccgacgcg gtccagcagc cgctcggccg tgccgccgat gaacacctgc
    12301 tcgagccggc tgcgggaaat gccgcccgcc accagcaggt cgatgccgag atcgtccacc
    12361 agcctgggca actcccgctc cgggcgacct tcggcgatgc gcaggcgcgc ggccggtatg
    12421 ccgcggcctt ccagcagtcg cagcaccgcc gcgcgcaagc gctgcaacag gtcgccggcg
    12481 tattcctccg gcgaggagcc gagcgcctcg ggcatcattc ccagcaccgc cgtggactcc
    12541 agcgacgcca tcgagagcat gcagtgcgcc acccaggcct cgccgcccag tgcctcgccg
    12601 aggcactcgg cctcgtccag gatggcgtga tcgagccgcg catgacggtc gtcccggtgg
    12661 ctcgggtcca ccgcagcgag cagtcttggt gtagccggcc aggccgcgcc cttcaccagc
    12721 agcagcggcg ccgggcagat gcgaatcagc tgccagtcgg ccgcgctcag cgtcgcgcgc
    12781 gccagccact cgtggtagtg cgtgcgcatc accacgtacg ctgcgccgac ggcgagcgca
    12841 cggcgcgcca cggcttcgtg acggggattg gcgtactcgg cggccgtacg cacttcgagc
    12901 ccgtccgcgc tcagcggcgc ggccacggac tccagccact cgcgccgcgc aacgacgcgc
    12961 gcctcccgcg cggcctcgag acgttcggtg acgcgaaaat ggctgccctc catcgcctgg
    13021 tcgaactcgc aggcgaacag ctcgagtggc gcaccaagcg ccgcggccac acgtcgcgca
    13081 cgcgccaggg cgtggcccgc cccgtccgcc gggtccatga cgaccagcac cggcgcgagc
    13141 ttggattcgt tcatcgggat tacctcagct gcgggctccg ccgctggccg tcaccatccg
    13201 gcaggaagga cccgcccagc gcctcgagtg cctcggcatc caggatctgc acctcgcgcc
    13261 ggcgcaccga caccagcccg tcttcctgga accgggcgaa cagccggctg atggtctcgg
    13321 tggcgagcct caggtggctg gcaaggtccc ggcgcggcat gggaagactc agcaggtcgg
    13381 gcggcaggcc gcgccgggcg tagcgcttcg acaggctgag caggaagccc gccagccgtt
    13441 cctcggcgct gtgatccgtg gccagttgcg cgctggccga cagctcgcga ctcatgatgc
    13501 ggagcatttg tgcctgcagg cccggcacct cgtggatcac ctgctcgagc cgcgcgaacg
    13561 gcaccgcaca cacggtcgtc gcctgcaggg cgaccgcatt gcagcgattg cgcccggagt
    13621 gaatgccgtc cagcccggcc acctcgccgg cgatgtggaa gcccagcacg tgctcgcggc
    13681 cctggttgtc gatggtgtaa gtcttcaccg cgccgctctt caccgcatag agcgcgtgga
    13741 aggcgtcgcc ttcccggaac aggtgttcgc cgctgcgaat cggcccgcga gaatgcacga
    13801 tggactcgag cttctgcatg tcgtccgcgc cgagccccag cggcaggcac aggtcgcgga
    13861 gcgagcaact gcggcagctc tggcgaagct gcgcgatgct gacgacattc gagtcgcgcg
    13921 cggcaggcat ggtttgcctc gaatattagc tactacggat tgtaaacaaa gacttgcacg
    13981 cgggtggcta gtgaaagcac ctatttaccc ttcggaagac cgcagtccag aattgatcca
    14041 aatcaactcc ggaattgtgc gcgtgattag aattccatgc aaattcaaca acaaagcatg
    14101 ggagttttgt catgcgtaca tttgcttcag cactcgcact ggcggccgcg ctcggcctgg
    14161 ccgcccccgc ttacggacac gaggcaggcg actggctggt ccgcttcggc gccagcaacg
    14221 tcgacccgaa gagcgacaac ggctcgatcg acctgacggc gaccgggatc ctcggcgtgc
    14281 aggacgtcac ggtcgacagc aaggtcggcg tgaccttcaa cgtggcctat atgtacaccg
    14341 acaccatcgg catcgaggtg cttgcggcgc tgcccttcaa gcacgacgtc aacgtggcgg
    14401 ggctgccgga gccggccgcc agcgtcacgc acctgccgcc gaccgtcagc ctgcagtact
    14461 acttcctccc gggctcgaat ttccggccgt acgccggcat cggcctgaac ctcacctggt
    14521 tcatggacga ggacaagaag gcgggcctgg cggcggccga cacggcgctc ggcgtgacca
    14581 cggacatcaa ggtggacagc acctccgtgg gcctggcggc acaactcggc ttcgactaca
    14641 agatcaacca gcagtggttc ctgaacgtcg acgtgcgcta catcgacatc agcacggatg
    14701 cgacgctgac cgtcaacggc gccaatttcg ccaccgccaa ggtcgacgtc gacccgctgg
    14761 tgtacggtat ccatctcggc tatcgcttct gagcccgatg cggggtatct tccccgcggc
    14821 gtcgaacgag tcccggggcg gcccgccgcc ccgggcacac tgcgaaaagc cgcgccgagc
    14881 gcggcttttc gcgtccggac ccgcgtccag cgatcgaatg aatcagcccg ggtgtgcgcc
    14941 tgcgccgccc gtaccatcct ccggcagccg gtagaggaca atgacgacag caagcgcata
    15001 caacgacaag gtcgtccgcc agttcaccat catgaccatc gtctggggag tggtcgggat
    15061 gctggtgggc gtgatcatcg ccgcccagct cctgtggccg gcgctcaatt tcgacatctc
    15121 gtggctcagc tacggccggc tgcggccgct gcacaccaac gcggtcatct tcgcgttcgg
    15181 gggctgcgcg ctgttcgcgt cttcgtacta tgtcgtgcaa cgcacctgcc atgtccggct
    15241 gttcggcggc ggcctggccg ccttcacctt ctggggctgg caggcggtaa tcgtgctcgc
    15301 ggccgtcacg ctccccctgg gcctgaccca gggcaaggaa tacgccgagc tcatctggcc
    15361 catcgacatc ctcatcgccg cggtgtgggt ctcctacgcg gtcgtgttct tcggcaccat
    15421 cgccgtccgc aaggtcaagc acatctacgt ggccaactgg ttcttcggcg ccttcatcct
    15481 cacggtcgcg gtgctgcaca tcatcaacag cctggccctg ccgaccagca tgctgcactc
    15541 ctacccggtg tattccggcg tggtggacgc catggtgcag tggtggtacg gccacaacgc
    15601 ggtgggcttc ttcctgacgg ccggcttcct cggcatgatg tattacttcg tgcccaagca
    15661 ggccgggcgg ccgatctact cctaccgcct gtccgtggtg catttctggg cgctgatctc
    15721 cctgtacatg tgggcgggcc cgcaccacct gcactacacc acactgcccg actgggcgca
    15781 gtcgctgggc atggtgttct cgctgattct cctggcgccc tcctggggcg gcatgatcaa
    15841 cggcatcatg accctgtccg gcgcgtggca taagctgcgc accgacccga tcctcaagtt
    15901 cctcatcgtg tcgctgtcct tctacggcat gtcgaccttc gaggggccga tgatgtcgat
    15961 caagacggtc aacgcgctgt cgcactacac cgactgga
//
LOCUS       DAOMPG010000028        15955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1586_length_15955_cov_4.358868, whole genome shotgun sequence.
ACCESSION   DAOMPG010000028 DAOMPG010000000
VERSION     DAOMPG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_15955_cov_4.358868"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLS34_02625"
     CDS             <1..1002
                     /locus_tag="PLS34_02625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRP86402.1"
                     /translation="RTKYTAVEGILELREAICEKFRRDNQIEYQPDQVLVSCGAKQSI
                     YNLAVALLGPGDEVIIPAPYWVSYPDIVLLADATPVIVSAGADQNFKITPAQLEAAIT
                     PRTRLLIINSPSNPTGVAYSRAELEGLAEVLRAHPQVITATDDIYEHIFWGAEPFCSL
                     LTAAPDLHERVVTINGVSKVYAMTGWRIGYAAGPRKLIGAMAKIQGQSTSNPASVAQY
                     ASLAALNGDQSCIAEMNAAFRARHDWLLEALNALPGFECLPADGAFYAFPDVRGAISA
                     ADTVDDDTGFAELLLDKAGVAVVPGTAFGAPGHLRLSYACSLDTLRAAVDRIAAALS"
     gene            1065..1140
                     /locus_tag="PLS34_02630"
     tRNA            1065..1140
                     /locus_tag="PLS34_02630"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Asn,seq:gtt)
     gene            1265..2674
                     /locus_tag="PLS34_02635"
     CDS             1265..2674
                     /locus_tag="PLS34_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86403.1"
                     /translation="MAEEAQAEREAGQANAGGAELARLRDRTDEIELIISGLTTFALF
                     TLPGWLFERIAEHYTHLSVTWLIGGTMMLVVLTGLCYGLGTCFLVHLLARAYWVGLIG
                     LRTAFPRGIDWQRTPGIGPVTRQRYRERLPDLQTAIERSDRLASSLFAVISVIALGIF
                     WIGALVAVTGTVGGLVGARFGATNVGIEIAFFTLIGLAAGTPVLLWLLDAGLAARWPR
                     LQQAEWFRTLLGGLARVNGWLVPQRLVLPVQLTLQSNTRPILFIMLFTLAIISIVMIG
                     QLSYGRATRFTLSNEFHYLGDAQLGGVAFRSSYYEDMRDGRDRLRAWPTIPSFEQRGS
                     AIRLFLPYRPLRDNLVLERLCPGEDAALGAGCLRRLWSVSLNGVAIDLGGFLAGERLD
                     LGMRGLVGAVPLEHLAPGIQVLTVVWNPDADEHDAPLDDRYSAARLGYEIPFLFAPDY
                     ERELESPGARRQPEGDPGQ"
     gene            complement(2721..3230)
                     /locus_tag="PLS34_02640"
     CDS             complement(2721..3230)
                     /locus_tag="PLS34_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRP86404.1"
                     /translation="MFEITSDQVDVEALRRGLMEPGCGGYTSFEGWVRNHHQGRAVSG
                     LHYEAYADLARAEGLKILAEAEERYGPVRLACVHRVGDLAVGDLAVWVGAAAPHRDEA
                     FKACRYVIDELKQRLPIWKKEHYIEGESAWVDCERCRAAGHGKHEHGHEHHHGHEHGP
                     RTRTEDEPA"
     gene            complement(3237..3482)
                     /gene="moaD"
                     /locus_tag="PLS34_02645"
     CDS             complement(3237..3482)
                     /gene="moaD"
                     /locus_tag="PLS34_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRP86405.1"
                     /translation="MSRQLQVSYFAWLRERAGCDDETISTTATTPAQLWTELDARHDF
                     ATERRHLRVAVNDAFSTWDAPLADGDRVVFIPPVSGG"
     gene            complement(3479..3961)
                     /gene="moaC"
                     /locus_tag="PLS34_02650"
     CDS             complement(3479..3961)
                     /gene="moaC"
                     /locus_tag="PLS34_02650"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRP86406.1"
                     /translation="MTRNKLTHLDERGRPRMVDVGGKAVTRRTAHARVAVAFPPEAAR
                     HFAENEYSTAKGPVFHTAIVAGVLAAKRTHELIPFCHPLGIEDCDIQIAMEGNEAVID
                     CRVSLHGQTGVEMEALTGASVAALTVYDMCKSLSKGLVIRETKLLEKTGGKSGDWRAA
                     "
     gene            complement(3967..5025)
                     /gene="moaA"
                     /locus_tag="PLS34_02655"
     CDS             complement(3967..5025)
                     /gene="moaA"
                     /locus_tag="PLS34_02655"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRP86407.1"
                     /translation="MKQTASTPQGTQTDSLGLDDRRGRPLKDLRISVMDRCNFRCPYC
                     MPENQYHRDFQFLHADERLSFGEITRLARLFVERGVTKLRLTGGEPLLRPGLVDLIGE
                     LSLIDGVEDLALTTNGILLAQHAAALKGAGLDRVTVSLDALDEATFRRMSGGRGTPER
                     VLEGIDAAVEAGLTPVKVNTVVQRGINERGIYALLERFRHTGVVLRFIEYMDVGTLNK
                     WNEKELVPSAELVERIRQRWAIRPIEAGYRGEVAERWEFEDGAGEVGFISSVTQPFCG
                     DCTRARLSSDGSLYTCLFAARGTDLRGPMRAGIEDVELGRMIDRVWTSRADRYSEQRA
                     EGGREVRTEKIEMYYIGG"
     gene            complement(5207..7351)
                     /locus_tag="PLS34_02660"
     CDS             complement(5207..7351)
                     /locus_tag="PLS34_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HRP86408.1"
                     /translation="MNGNRTLKVIVAALLGAGTAAGPAVMAQEMPDFEQWQCRFCPFP
                     EKGVSGSADASVLNVSDDSARFGNYTGLDEKGTYVNADADIQYRADGGYAVSLYARDL
                     GLDSRLIELSAGRQGSWVVDLTWDEIPRRLDDSGRTVYSGLGSDSLVLPTGWVRGNFT
                     SDMSALDASLRDFTLGWDRKTAGLGLEFVQSERLRYEADWTQQTKRGRGLTWGNFLGT
                     AEDLVKPLDYQTDQVDAALIYTGDGWNVRAGYYGSFFSNKDLWLTWQNPFNGADSGRS
                     ALAPDNRYHQGQLSGAYRFDTWDTTLNASYAFGRMEQTDTLLAYTINPAIAIQPLPLD
                     KFDGRVDTTAANLRLTARPMERLRVTGEYRYTERDNKSGQYDWAAVQADSFPTLPFRN
                     PAYSFENTDWSLAADYRFNEMIAGAAGVDRKIRKRDYQNVARTEDDSVWARVRFRPAS
                     ALALSVKAESASRDASDYQQLPTTGAGAEQNPLLRKYYLTDRDRDLLQVQLDVVPGAR
                     GSISMRYEDARDRYDDSIVGLVASDYDQFSADASVQLWKSLVMTAYFSRENYDSDMVG
                     AASFLAPNLAGPNWRGRTNDRHDVLGVVFSLPGLMDGKLDLTADWSEADTRGDISIEN
                     PLGVAGGAFPSLRSRLTGTQLTADYHLNPRWTLNAGWRREKFSADDWSKDGVGPATIA
                     SVLTFGAQTLDYDVDVFLLGFRYHFVKDKTEE"
     gene            complement(7373..8305)
                     /locus_tag="PLS34_02665"
     CDS             complement(7373..8305)
                     /locus_tag="PLS34_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HRP86409.1"
                     /translation="MLLRACFVSIALLLLGVADFARAQDEAPSYSRRGADGCIGCHSD
                     WEPPLMGLFQTPHGNPHDARSPFGEQQLQCEACHGPGATHGGRVPRGQPRPPIINFGS
                     DAWTPVPQQNAMCLACHDGHVGQQWAGNVHDVNNVACADCHTLHTPKDAMLDARTQSE
                     ACYACHAEVRSDFLKPSAHPVRQGQLGCTDCHNPHGTNTEKLLVRATLNETCFSCHAE
                     KRGPLVWEHAPVAEDCSICHDPHGAVHASMLTRRAPLLCQSCHTPSGHPSVPYDSGSL
                     AGGTASPFVLAGSCLSCHTQVHGSNHPSGKALMR"
     gene            complement(8371..10677)
                     /locus_tag="PLS34_02670"
     CDS             complement(8371..10677)
                     /locus_tag="PLS34_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HRP86410.1"
                     /translation="MTGKNGRFSGAGALAALGLLSLALAGCSGSDGPAGPAGPTGPTG
                     PTGPVGDSGAILIGNGSTLNAAQVAALGRIVARIDSVAMSTTPVVEFTLFSETGIRIL
                     GLAPNTLGFTLAKLSAGSSGEQWVSYINRVQTAGATTPQVLPQAVQANTESGAAGTLE
                     ELGEGRYRYTFATNPANVTSPVPVSYEPSVVHRVGLEIRLQAPANRIFPDNPVYDFIP
                     ATGAAVPLARTIADNANCANCHERLEFHGGPRVTVEYCVTCHNPGTVDPDSGNSLDMV
                     YMTHAIHMGALRAAPYVIYGFGGTPHDYSHVTYPQSPLFCETCHEASDVTPLGDTWNA
                     TATASSCGGCHADGLLVGTPDAVTGRSSYAYQHSFGAAVDGTCVSCHAPGGPAGANAD
                     NHLRGAREAVQIGREQFAYEVVEVSDTAPGERPVITFAVNNPAAGTRYDINSDAPFNQ
                     GAASLTLNIAWNTGDYSNEGSGSAGPASGAPAQPFALNLAFLKANATRNADGSYTVTA
                     PEPLPAAATGGVAVALEGRPSVALVSTGVLTNIPVKGDVAFAGAPRREIVAIDSCNAC
                     HESLALHGNNRADNVQLCATCHNPDATDIRRREGFSWAAPSPLDGKGEESVDMRYMIH
                     AIHAAQTVVYGFGNTAHDYREVGYPQSPGNCLACHAPGTYYPTPPTARSVTINTGADR
                     SDWRDDVAMTPTAAACWACHQASSSSPPELVKIHIELSGGYLPDPTDTSVTKEMLESH
                     ATSTYLETCGVCHGQGAPFDVGVLHGLL"
     gene            complement(10955..11827)
                     /gene="galU"
                     /locus_tag="PLS34_02675"
     CDS             complement(10955..11827)
                     /gene="galU"
                     /locus_tag="PLS34_02675"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRP86411.1"
                     /translation="MSSTIRYAVFPVAGKGTRFLPATKASPKEMLPVVDKPLIQYAVE
                     EAIAAGAVNLVFITGRAKRAIEDHFDVEPELEQLLASKDKEALRDMVRNIVPPHVNCI
                     YVRQGEPLGLGHAVLCARPVVGEQPFFVHLADDLIDAQEACLGQMARVFDATGGSVLG
                     VEEVPPDQTRQYGIIDGEQAQAGLRRLRGIVEKPRPEAAPSNLAVVGRYILTPRIFAL
                     LEDTRAGAGGEIQLTDAIARLLREEAVYGFEFEGRRFDCGSKLGYLEATVHYGLKHPE
                     LGAEFRAHLEGLFG"
     gene            complement(11942..12220)
                     /locus_tag="PLS34_02680"
     CDS             complement(11942..12220)
                     /locus_tag="PLS34_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRP86412.1"
                     /translation="MIRIAKTLLAILALLPVMAWAGPVDINTADAATLARELKGVGPA
                     RAEAIVAWRDANGPFRSPEDIVLVQGIGERVLEDNRAILTVGEQAEDE"
     gene            complement(12310..13533)
                     /gene="lapB"
                     /locus_tag="PLS34_02685"
     CDS             complement(12310..13533)
                     /gene="lapB"
                     /locus_tag="PLS34_02685"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HRP86413.1"
                     /translation="MSVADTLLLLLLFAAAAGLGWYFARRTGTRAGPGRPRQVSAEYF
                     QGLNYLLNEEPDKALEVFIRMVDMDADTVDTHFALGALFRRRGETERAIKVHQNLVAR
                     PDLPREARARALNELAEDYFKAGLFDRAEQVYAQVARGTAQREQGLRGLVRLYELQRD
                     WEKAIAAMDQLVSSARDANGTVVAHYCCELAQEAFNRGDMLAARHWLKRAQGADRDGV
                     RGAMMRAAVAQREGDHRLAVRLYEQALERDGGFLADVLPALRLSYMELGKRDAFERWL
                     GEALARHPEWRPGVAFAAITDGTFDDQVTQQCIADFVSGNPILTEILETLRPTLESEG
                     GKEAAIRRISRAMRRIAKSSPRYRCNVCGFSGNALYWQCPSCRSWDTTRPVTRFRFEA
                     MLDPRVVPPESVRKR"
     gene            complement(13520..13804)
                     /locus_tag="PLS34_02690"
     CDS             complement(13520..13804)
                     /locus_tag="PLS34_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HRP86414.1"
                     /translation="MVKRIFTAVIFLTALALSAFFTSLNPGEINLDLGFAALKAPLGL
                     AFVAALALGWLLGVLSLFGWAARLAADRRRLRGELKRASAGGFAGQDERS"
     gene            complement(13887..14189)
                     /locus_tag="PLS34_02695"
     CDS             complement(13887..14189)
                     /locus_tag="PLS34_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRP86415.1"
                     /translation="MTKSELIEAIARRQSHLNAKDVELAVKYLIEVMSNSLASGERIE
                     IRGFGSFSLHFRPPRIGRNPKTGEAVALSGKYVPHFKPGKDLRERVNEGRDHPIKA"
     gene            complement(14278..15954)
                     /gene="rpsA"
                     /locus_tag="PLS34_02700"
     CDS             complement(14278..15954)
                     /gene="rpsA"
                     /locus_tag="PLS34_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRP86416.1"
                     /translation="MSESFAELFEQSFASQQIRPGAILMGRVVEINDDVVIVSAGLKS
                     ESVIPAEQFKNERGEWEVAVGDEVEVALDAVEDGFGETRLSRDKAKRSRTWTVLEEAF
                     EKGEIVQGMITGRVKGGFTVELELVRAFLPGSLVDVRPVRDPSYLENKMLEFKVIKLD
                     RKRNNVVVSRRAVVEAESSVEREELMKNLTEGAVLPGIVKNLTDYGAFVDLGGIDGLL
                     HITDMAWRRVKNPSEVVNVGDEIQVKVLKFDRERNRVSLGLKQLGEDPWQDIARRYPT
                     GTRLFGKVTNLADYGAFVEIEDGVEGLVHVSEMDWTNKNVNPSKIVQIGDEIEVMVLE
                     IDEERRRVSLGIKQCMPNPWNEFATSFNKGDRVKGVIKSITDFGIFIGLDGGIDGLVH
                     LSDLSWDLPGEEAVRNYRKGEEVEAVVLSIDPERERISLGVKQMDRDPMSAWLAENPK
                     GSIVKGVVTEVDAKGAIVDVGGLEGYLRASELSRERVEDARAVLKQGEEIEARFIGLD
                     RKSRQISLSIKAKEVWEEAEAMQSYKKSDTTTGTSLGDLLKEQISSQENS"
BASE COUNT         2447 a         5487 c         5269 g         2752 t
ORIGIN      
        1 cggaccaagt acaccgcggt cgagggcatc ctcgaactgc gcgaggccat ctgcgagaag
       61 ttccgccgcg acaaccagat cgagtaccag cccgaccagg tgctggtctc ctgcggtgcc
      121 aagcaaagca tctacaacct cgccgtcgca ctgctcggcc ccggcgacga ggtcatcatc
      181 ccggcgccgt actgggtctc ctatcccgac atcgtgctgc tcgccgacgc cacgccggtg
      241 atcgtctccg ccggcgcaga ccagaacttc aagatcacgc cagcgcagct cgaggcggcg
      301 atcacgccgc gcacccgcct gctgatcatc aacagtccga gcaacccgac cggcgtcgcc
      361 tacagccgcg ccgaactgga agggctcgcc gaggtactgc gggcacatcc gcaggtcatc
      421 accgccacgg acgacatcta cgagcacatt ttctggggag cggagccgtt ctgcagcctg
      481 ctgaccgcgg ccccggacct gcacgagcgc gtggtgacca tcaacggcgt gtccaaggtc
      541 tacgccatga ccggctggcg catcggctac gccgccggac cacgcaagct gatcggcgcg
      601 atggcgaaga tccagggcca gagcacctcc aacccggcct cggtcgccca gtatgcgtcc
      661 ctggctgcgt tgaacggcga ccagtcctgc atcgccgaga tgaacgccgc gttccgcgcc
      721 cgccacgact ggctgctgga ggcgctcaac gccctgcccg gcttcgagtg cctgcctgct
      781 gacggggcct tctacgcctt cccggacgtc cgtggcgcca tttcggctgc cgacaccgtc
      841 gacgacgaca cgggatttgc cgaactcctg ctcgacaagg ccggcgtggc ggtcgtgccg
      901 ggcacggcgt ttggcgcgcc gggccacctc cgtctgtcct acgcctgctc gctggacacc
      961 ttgcgagcag ctgtcgacag gatcgccgcg gcgctttctt gacaccatca tccgccttgc
     1021 cggaaaatta cctgcttggg cggcgctgat ccgccactga tttttccccg gtagctcagt
     1081 tggtagagcg acggactgtt aatccgttgg tcgctggttc gagtccggcc cggggagcca
     1141 ggcaccgaga agggctgcgc cgaatgcgca gcccttttct tttgcgcagg ggacgcattt
     1201 cccggctcat cggctaggat gtaggccgcc ccgcactgtg cggacacggg cgaggagctg
     1261 ccgaatggct gaagaggcgc aggccgaacg cgaagctggc caggcgaacg ccggcggcgc
     1321 cgaactcgcg cgactgcgcg accgcacgga cgagatcgaa ctgatcatct ccggcctgac
     1381 cactttcgcg ctgttcacgc tcccgggctg gctgttcgag cgcatcgccg agcactacac
     1441 gcacctgtcg gtgacctggc tcatcggcgg cacgatgatg ctcgtcgtgc tcaccggttt
     1501 gtgctacggc ctgggcacct gcttcctggt ccacctgctg gcgcgcgcct actgggtggg
     1561 actgatcggg ctgcgcacgg cctttccccg cggcatcgac tggcagcgta cgccgggcat
     1621 cgggccggtc acccggcagc gttatcgcga gcgcctgccg gacctgcaga cggcgatcga
     1681 gcgctccgac cggctggctt cctcgctgtt cgcggtgatc agcgtcatcg cgctcggcat
     1741 cttctggatc ggtgcgctgg tggcggtgac cggcaccgtc ggtggactgg tgggcgcccg
     1801 cttcggcgcg accaacgtcg gcatcgagat cgccttcttc accctgatcg ggctggcagc
     1861 gggcaccccg gtgctgctgt ggctgctgga cgccgggctt gcggcccgct ggccgcggct
     1921 gcagcaagcc gagtggttcc gcacactgct cggcggtctg gccagggtca acggctggct
     1981 ggtgccgcag cgactcgtac tgcccgtgca gctcaccctg caaagcaaca cgcgtccgat
     2041 cctgttcatc atgctgttta cgctcgcgat catctcgatc gtcatgatcg gccagctgag
     2101 ttacgggcgt gcgacccgct ttacgctctc gaacgagttc cactacctcg gcgatgcaca
     2161 gcttggcggc gtcgccttcc gcagcagcta ttacgaggat atgcgcgacg gccgggaccg
     2221 gctgcgtgcc tggcccacca ttccgtcctt cgagcagcgc ggcagcgcca tccggctgtt
     2281 cctgccctat cgcccgctgc gcgacaacct ggtgctggag cggctctgcc ccggcgagga
     2341 cgccgcgctg ggcgccggct gcctgcggcg gttgtggtcc gtgtcgctga acggcgtggc
     2401 gattgacctc ggcggcttcc tggcggggga gcgtctcgat ctgggaatgc ggggcctggt
     2461 gggcgccgtc ccgctcgagc acctcgcacc cggcatccag gtgctgaccg tggtctggaa
     2521 cccggatgcc gacgaacacg acgccccgct ggacgatcgc tattcagcgg cgcgactcgg
     2581 ctacgaaatt cccttcctgt tcgcgcctga ctacgagcga gagctcgaat caccgggcgc
     2641 gcgccgccag cctgaaggcg acccgggcca ataacgccgc acgcgtgtcc gggagtcttt
     2701 gtgcgccgcc acgtcgcgtg tcaggccggt tcgtcctcgg tccgggtcct ggggccgtgt
     2761 tcatggccgt ggtggtgttc atggccgtgc tcgtgcttgc cgtgcccggc cgcccgacag
     2821 cgctcgcaat ccacccaggc cgactcgccc tcgatgtagt gctctttctt ccagatcggc
     2881 agccgctgct tgagctcgtc gatgacatag cggcaggcct tgaaggcctc gtcgcgatgc
     2941 ggcgcagcgg cgccgaccca gacggccagg tcgcccacgg ccaggtcgcc gacgcggtgc
     3001 acgcaggcca gcctcaccgg cccgtagcgc tcttccgctt cggccaggat cttcaggccc
     3061 tcggccctgg cgagatcggc ataggcctcg tagtgcaggc cggataccgc gcggccctgg
     3121 tgatggttgc gcacccaccc ctcgaaggag gtgtagccgc cgcatcccgg ctccatcagg
     3181 ccgcggcgca gcgcctcgac gtccacctgg tcgctggtga tctcgaacat cgtggttcag
     3241 cctcccgata ccggcgggat gaatacgacc cggtcgccgt ccgccagcgg cgcatcccag
     3301 gtcgagaaag cgtcgttgac ggccacccgc aaatggcggc gctcggtcgc aaaatcgtga
     3361 cgcgcatcca gctcggtcca cagctgcgcc ggcgtcgtgg cggtcgtgct gatcgtctcg
     3421 tcgtcacagc cggcgcgttc gcgcagccag gcgaaatagc tgacctggag ttgtctgctc
     3481 atgccgcccg ccagtcgccg ctcttgccgc cggtcttctc cagcagcttc gtttcgcgga
     3541 tcaccaggcc cttggataac gatttgcaca tgtcgtagac ggtaagcgcg gccacgctcg
     3601 cacccgtcaa ggcctccatt tcaacgccgg tctggccgtg caggctgaca cggcaatcga
     3661 tcaccgcctc gtttccctcc atggcgatct ggatgtcgca atcctcgatg cccagcggat
     3721 ggcaaaaggg aatcaactca tgggtgcgct tggccgccag cacccccgcc acgatggcgg
     3781 tatggaagac cggtcccttg gctgtcgaat actcgttctc ggcgaagtgc cgcgcggcct
     3841 ccggcgggaa cgccaccgca acgcgcgcat gcgccgtgcg ccgggtgacc gccttgccgc
     3901 cgacatcgac catgcgaggc cggccgcgtt catcgaggtg ggtgagcttg ttccgtgtca
     3961 tgccggctat ccgccgatgt agtacatctc gatcttctcg gtgcggactt cgcgcccgcc
     4021 ctccgcccgc tgctcgctgt agcggtcggc acgactggtc cagacgcgat cgatcatgcg
     4081 gccgagttcg acgtcctcga tgcccgcccg catgggaccg cgcaggtccg taccacgggc
     4141 cgcgaacagg cacgtgtaca agctgccgtc cgacgacaat cgcgcacggg tgcagtcgcc
     4201 gcagaacggc tgggtcaccg agctgatgaa gccgacctcg cccgcgccgt cctcaaactc
     4261 ccagcgctcg gcgacctcgc cgcggtagcc cgcctcgatc ggccggatcg cccagcgttg
     4321 cctgatgcgc tcgaccagct ccgccgaggg caccagctcc ttttcgttcc atttgttgag
     4381 cgtgccgacg tccatgtact cgatgaagcg cagcacgacg ccagtgtgac ggaaccgctc
     4441 cagcagcgca tagatgccgc gttcattgat gccgcgctgg acgaccgtgt tgaccttgac
     4501 cggcgtgagg ccggcctcga cggcggcatc gatgccctcc aggacgcgct ccggggttcc
     4561 ccgcccgccg ctcatgcggc ggaaggtcgc ctcgtcgagg gcatcgaggc tgacggtcac
     4621 ccgatcgagc ccggcaccct tcagcgcggc ggcatgctgc gccagcagga tgccgttggt
     4681 ggtcagggcg aggtcttcga cgccgtcgat caggctcagt tcgccgatca ggtcgaccag
     4741 gccgggccgc agcagcggct cgccgccggt gagccggagc ttggtgacgc cgcgttccac
     4801 gaacaggcgt gccagccggg tgatctcgcc gaaggacaaa cgctcgtcgg catggaggaa
     4861 ctggaagtcc cggtggtact gattttccgg catgcagtaa gggcagcgga aattgcaccg
     4921 gtccatcacc gagatgcgga gatccttgag tggacgcccg cgccggtcat caaggccgag
     4981 cgagtcggtc tgcgtgccct gcggcgtact ggccgtctgc ttcactaccc tcctcccccg
     5041 gaatcatgat tatcggcaaa ttatcgcatg gcccccaggc aggatgccac cagacgctgg
     5101 cgcgctggat gctgcgtcgc gcaatcatcc cggacaatcc tcaatgcaag aaaaagggcg
     5161 ccctctcggg cgccctcgcg gttcgatcaa cagcgctgcc ccaggctcac tcctcggtct
     5221 tgtctttcac gaagtgatag cggaaaccca gcagaaacac gtcgacgtcg taatcgagcg
     5281 tctgcgcgcc gaaagtcagc acgctggcga tggtcgccgg gccgacgccg tccttcgacc
     5341 agtcatccgc cgagaacttt tcgcgtcgcc agccggcgtt cagcgtccaa cgcgggttca
     5401 ggtggtagtc ggccgtcagt tgggtcccgg tgagccggga gcgcagcgac gggaacgcgc
     5461 cgccggcgac tcccagcggg ttctcgatcg agatgtcccc gcgcgtgtcc gcttcgctcc
     5521 agtccgccgt caggtccagc ttgccatcca tcaggccagg caggctgaac accacgccga
     5581 gcacgtcgtg gcgatcattg gtgcggcctc gccagttcgg tcccgctagg ttcggcgcga
     5641 ggaagctggc cgccccgacc atgtcggaat cgtagttctc ccggctgaaa taggccgtca
     5701 tcaccaacga cttccacagc tgcacgctgg cgtcggcgct gaactggtcg taatcggaag
     5761 ccaccagccc gacgatgctg tcgtcgtacc ggtcccgggc gtcctcgtag cgcatcgaga
     5821 tgctgccgcg cgcccccggc acgacgtcga gctgcacctg caacaggtcg cggtcgcgat
     5881 ccgtcaggta gtacttgcgc agcagcgggt tctgctccgc ccccgccccg gtggtgggca
     5941 actgctggta gtcggaggca tcgcgcgatg cgctctccgc cttgacgctg agcgccagcg
     6001 cgctggccgg gcggaagcgc acgcgcgccc atacgctgtc gtcctcggtg cgcgcgacgt
     6061 tctggtagtc gcgcttgcgg atcttgcggt cgacgcccgc ggcgcccgca atcatctcgt
     6121 tgaaacggta atccgccgcc agcgaccagt cggtgttctc gaagctgtag gccggattgc
     6181 ggaacggcag cgtcgggaag gaatcggcct gcaccgcggc ccagtcatac tgccccgact
     6241 tgttgtcccg ttccgtgtaa cggtattcgc cggtaacccg cagccgctcc atgggccgcg
     6301 cggtcagccg gaggttggcg gcggtcgtgt ccacccgtcc gtcgaacttg tccagcggta
     6361 gcggctggat cgcgatcgcc ggattgatgg tgtaagcgag cagggtgtcg gtttgttcca
     6421 tccggccgaa ggcgtagctg gcgttcagcg tcgtgtccca ggtgtcgaaa cggtaagccc
     6481 cggacagctg gccctggtga tagcggttgt cgggggccaa cgccgagcga ccgctgtcgg
     6541 ccccgttgaa gggattctgc caggtcagcc agaggtcctt gttgctgaag aaggagccgt
     6601 aataaccggc ccggacgttc cagccgtcgc ccgtgtagat gagcgccgcg tcgacctggt
     6661 cggtctggta atccagcggc ttgaccaggt cctcggccgt gccgaggaag ttgccccaag
     6721 tcaggccccg accccgcttg gtctgctgcg tccagtcagc ctcgtagcgc aggcgctccg
     6781 attggacgaa ctccagcccg agccccgcgg tcttccgatc ccatcccagc gtgaaatccc
     6841 gcaggctggc atcgagcgcg gacatgtcgc tggtgaaatt gccgcgcacc cagccggtcg
     6901 ggagcacgag cgaatcgctg ccgaggccgc tgtaaaccgt gcgcccggag tcgtcgagcc
     6961 gccggggaat ttcgtcccag gtcaggtcta ccacccagct tccctgccgt ccggcgctca
     7021 gctcgatcag gcgtgaatcc agcccgaggt cgcgcgcgta cagtgacacg gcatagccgc
     7081 cgtcggcgcg gtactggatg tccgcgtcgg cgttgacgta agtgcccttc tcgtccagcc
     7141 ctgtgtagtt gccgaaacgc gcggagtcgt ccgagacgtt cagcacggag gcgtccgcgc
     7201 taccgctcac gcccttctcc gggaacggac agaaacggca ttgccactgt tcgaagtccg
     7261 gcatctcctg cgccattacg gcaggccccg ccgcggtccc ggcgcccagc agcgccgcca
     7321 cgatgacctt gagggtacgg tttccgttca tttcatgcgc ctcctgcgac gctcagcgca
     7381 tcaacgcctt gccggacggg tgattcgagc cgtgcacctg ggtgtggcag ctcaggcagc
     7441 tgccagcgag cacgaacggc gaggccgttc cgccggcgag cgaaccgctg tcatagggca
     7501 cggacggatg cccgctcggg gtgtggcacg actggcacag caacggggcg cgccgcgtca
     7561 gcatggaagc atgcacggcg ccgtgggggt cgtgacagat gctgcagtct tcggccaccg
     7621 gcgcgtgctc ccagaccagc gggccgcgtt tctcggcatg gcagctgaag caggtctcgt
     7681 tgagggtggc gcggaccagc aacttctcgg tgttggtgcc gtgcggattg tggcaatcgg
     7741 tgcagcccag ctggccctgg cggaccggat gggccgaggg cttcaggaag tcgctccgga
     7801 cctcggcgtg gcaggcgtag caggcctcgc tctgggtgcg cgcgtcgagc atggcgtcct
     7861 tcggcgtatg cagcgtgtgg cagtcggcgc aggctacgtt gttgacgtca tgcacgttgc
     7921 ccgcccactg ctgaccgacg tggccgtcgt ggcacgccag gcacatcgcg ttctgttgcg
     7981 gaaccggcgt ccacgcgtcc gagccgaaat tgatgatcgg cggacgcggc tggccgcgcg
     8041 gcacccgacc gccgtgcgtc gcccccggcc cgtggcaggc ctcgcactgc agctgctgct
     8101 cgccgaacgg cgaacgggcg tcgtgcggat tgccgtgcgg cgtctggaac agccccatca
     8161 atggcggctc ccagtcgctg tgacagccga tgcagccgtc tgcgccgcgc cggctgtagc
     8221 tcggggcctc gtcctgtgcc cgtgcgaagt cggctacgcc gagcagcaac agggcgattg
     8281 aaacaaagca cgctctcaac agcatcttgc gtcccttccg gtaagccgcg acctcccgcc
     8341 cggtgatcgg ccggggggcc cggaaatgaa tcaaagcagc ccgtgcagca cgccgacgtc
     8401 gaacggagcg ccctggccat gacatacgcc acaggtctcg aggtaagtcg aggtggcgtg
     8461 cgattcgagc atttccttgg tgacgctggt gtccgtcggg tccggcaggt agccgccact
     8521 gagctcgata tggatcttca ccaactccgg gggagaactc gaacttgcct ggtgacaggc
     8581 ccagcaggcc gccgccgtcg gcgtcatcgc cacgtcgtca cgccagtcgg aacgatcggc
     8641 gccggtgttg atggtcaccg agcgcgccgt gggcggggtc gggtagtagg tgcccggagc
     8701 gtgacatgcc aggcagttgc cggggctttg cgggtaaccg acctcgcgat agtcgtgggc
     8761 cgtgttgccg aagccgtaga ccacggtctg cgctgcgtgg atcgcgtgga tcatgtagcg
     8821 catgtcgacg ctctcctcgc ccttgccgtc cagcggcgaa ggcgcggccc agctgaagcc
     8881 ctcacgccgt cggatgtcgg tggcgtccgg gttgtggcag gtggcgcaga gttgcacgtt
     8941 gtcggcgcgg ttgttgccgt ggagcgcgag cgactcgtgg cacgcgttgc agctgtcgat
     9001 ggctacgatt tcgcgccgcg gcgcgccggc gaatgcgacg tcgcccttga ccggaatgtt
     9061 ggtcagcacg ccggtcgaca ccagcgccac cgaggggcgg ccctcgagcg caaccgccac
     9121 gccgccggtg gctgccgccg gcaagggctc gggcgccgtg accgtgtaac tgccgtcggc
     9181 attgcgcgtc gcgttggcct tgaggaaggc gaggttcaag gcgaatggct gcgcgggcgc
     9241 gccgctggcc gggccggcgc tgccgctgcc ctcgttcgag tagtcgccgg tgttccaggc
     9301 gatattgagc gtcaggcttg cggcgccctg gttgaacggc gcatcgctgt tgatgtcgta
     9361 acgcgtgccc gccgcggggt tattgaccgc gaacgtaatc accggccgtt cgccgggcgc
     9421 cgtgtcgctg acctcgacga cttcgtaggc gaactgctcg cgcccgatct gcacggcttc
     9481 cctggcgccg cgcaggtggt tgtcggcatt tgccccggcc ggaccgcccg gcgcgtggca
     9541 gctgacgcaa gtgccgtcca ccgccgcgcc gaagctgtgc tggtaggcat atgaggagcg
     9601 gcccgtgacc gcgtcgggcg tgccgaccaa aagcccgtcg gcgtggcagc cgccgcacga
     9661 ggatgccgtc gcggtcgcgt tccaggtgtc gccgagcggc gtcacgtcgc tggcctcgtg
     9721 acaggtctcg cagaacagcg gcgactgcgg ataggtcacg tgcgaatagt cgtgcggcgt
     9781 gccgccgaaa ccgtagatta cgtagggggc ggcgcgcaac gcgcccatgt ggatggcatg
     9841 ggtcatgtac accatgtcca gcgagttgcc ggaatccggg tcgaccgtgc ccggattgtg
     9901 gcaggtcacg cagtattcca ccgtgacgcg cggcccgccg tgaaactcga gccgctcgtg
     9961 gcagttggcg cagttggcgt tgtccgcaat cgttcttgcc agcggcaccg cggcgccggt
    10021 ggccgggatg aagtcgtaga ccgggttgtc ggggaagatt ctgttggccg gcgcctgcag
    10081 gcggatttcc agcccgacgc gatggaccac gctcggctcg tagctcaccg gcaccgggct
    10141 ggtgacgttc gccgggttgg tggcgaaagt gtagcgatag cggccctcac ccagttcctc
    10201 gagcgtgccg gccgcgccgg actcggtatt ggcctgcacg gcctgcggca gcacttgggg
    10261 cgtcgtcgcg cccgccgtct gcacccggtt gatgtaactg acccattgct cgccgctgct
    10321 gccggccgac agcttggcca gcgtgaagcc gagcgtgttg ggggcgagcc cgaggatccg
    10381 gatgcccgtc tcgctgaaca gcgtaaactc gaccaccggc gtagtgctca tggcgaccga
    10441 gtcgatccgc gccacgatgc gcccgagcgc cgccacctgt gccgcgttga gcgtgctgcc
    10501 gttgccgatg aggatggcgc ccgagtcacc gacgggcccg gtggggccgg tcggaccggt
    10561 ggggcctgcg ggtccggcgg ggccgtcact gccgctgcaa cccgccagtg cgagggaaag
    10621 caagccgagt gcagccagtg cgcccgcgcc cgagaaacgt ccgttcttac cggtcattga
    10681 tggtatcccc aatccgcaac attggaccca ggcgccgccc agggcgcggc aactcgcgga
    10741 atttcttcaa gttgacccaa gcatttcagt ggagatgggc gcaattcagt atcagggctt
    10801 atacctaact ccgcgcaatt tatgcattcc agatgaataa atccaggcat ggcagggaaa
    10861 ttgtgcgctg cggcagccag cgtatgggcc tcgtccaggc gagaggggcc ctgctgaacc
    10921 ggccgcttcc gcgcggggcg acccgacgcc cggctcagcc gaacaggccc tcgaggtggg
    10981 cgcgaaactc cgcaccgagc tccggatgct tcaagccgta atggacggtg gcctccaggt
    11041 agccgagctt gctgccgcag tcgaatcgcc ggccctcaaa ctcgaagccg tagaccgctt
    11101 cctcacggag caggcgggcg attgcgtcgg tgagctggat ttcgccgccg gcgccggcgc
    11161 gggtgtcttc cagcaacgcg aatatccgcg gcgtcaggat gtagcggccc acgacggcga
    11221 gattggacgg tgccgcctcg ggacggggct tctcgacgat gccgcgcagg cgccgcagcc
    11281 cggcctgtgc ctgctccccg tcaatgatgc cgtactggcg ggtctggtcg gggggcacct
    11341 cctccacgcc cagcacgctg ccacccgtgg cgtcgaacac ccgggccatc tggccgagac
    11401 aggcctcctg ggcgtcgatg aggtcgtccg ccaggtgcac gaagaacggt tgctcgccca
    11461 ccaccgggcg agcgcagagc acggcgtggc ccagccccag cggctcgccc tgccggacat
    11521 agatgcaatt cacgtgcggc ggcacgatgt tgcgcaccat gtcacggagc gcttccttgt
    11581 ccttgctggc gagcagctgc tcgagctcgg gctcgacgtc gaagtggtcc tcgatcgccc
    11641 gcttcgcccg cccggtgatg aaaaccaggt tgaccgcgcc cgccgcgatc gcctcttcca
    11701 ccgcgtactg gatcagcggc ttgtccacca ccggcagcat ttccttgggg ctggccttgg
    11761 tggcggggag gaagcgcgtt cccttgccgg ccaccgggaa tacggcgtag cgaatggtgc
    11821 tgctcaaggc gcgcctcctg catgtgcggt tccgtggacc gcggcgatgc tggcacatcc
    11881 cggcaggcac gcgcaacgcc acgggcggcg gccggctgcg ggccgccgcc cgcgtgtcgc
    11941 gtcattcgtc ctcggcctgc tcgcccaccg tgagtatcgc gcggttgtcc tccagcacgc
    12001 gctccccgat gccctggacc aggacaatgt cctccggtga ccggaacggc ccattggcgt
    12061 cgcgccaggc gacgatcgcc tcggcgcggg ccggcccgac gcccttgagc tcgcgcgcca
    12121 gcgtcgccgc atcggccgtg ttgatgtcca ccggtccggc ccaagccatg accggcagca
    12181 atgccagtat ggccagcaat gtcttcgcga ttcgaatcat ttcatgctcc cttgtgacga
    12241 agatgcgcgg ccggatcggc cgcaacgcca atcgtccgtc ctgcaggggc cggcgaggag
    12301 gtggaaaaat cagcgcttgc gcacggattc ggggggaacc acccgcgggt cgagcatcgc
    12361 ctcgaaccgg aatcgggtga ccggccgggt ggtgtcccag ctgcggcagc tcggacattg
    12421 ccagtacagg gcattgccgg agaagccgca gacgttgcag cggtagcgag ggctcgattt
    12481 ggcaatccgg cgcatggcac ggctgatgcg ccggatcgcg gcttccttgc ccccctcgct
    12541 ctcgagggtc ggccgcaagg tctcgaggat ttcggtcagg atcggattgc cgctcacgaa
    12601 gtcggcaata cactgttgcg tcacctggtc gtcgaaggtg ccgtccgtga tggcggcaaa
    12661 ggccacgccg ggccgccatt cggggtgcct cgccagggcc tcgcccagcc agcgctcgaa
    12721 cgcgtcacgc ttgccgagct ccatgtagct caggcgcagc gccggcagga cgtcggcgag
    12781 aaagccgccg tcacgctcca gggcctgttc gtagagcctg accgcgaggc ggtgatcacc
    12841 ctcgcgctgc gccacggccg cgcgcatcat ggccccgcgc accccgtcgc gatcggcccc
    12901 ctgggcgcgc ttcagccagt gccgggccgc cagcatgtcg ccacgattga aggcctcctg
    12961 cgccagttcg cagcagtaat gcgccaccac ggtcccgttc gcgtcgcgcg cactcgacac
    13021 cagctggtcc atggcggcga tggccttctc ccagtcccgc tgcagctcgt acaggcgcac
    13081 cagccctcgc agcccctgct cccgctgcgc cgtaccgcgt gccacctggg cgtaaacctg
    13141 ctcggcccga tcgaacagcc cggccttgaa gtaatcctcc gccagctcgt tcagggcgcg
    13201 cgcccgggcc tcgcgaggca ggtccgggcg ggccaccagg ttctggtgca ccttgatggc
    13261 gcgctccgtc tcgccgcgcc gccggaacag cgcgccgagc gcgaagtggg tatcgacggt
    13321 gtccgcgtcc atgtccacca tgcggatgaa cacttccagg gccttgtcgg gctcctcgtt
    13381 gagcaggtag ttcaggccct ggaagtactc cgcgctgacc tgccgggggc ggcccgggcc
    13441 ggcgcgggta cccgtgcgcc gcgcgaagta ccagccgagg cccgccgccg cggcgaacag
    13501 cagcagcaac aggagcgtgt cagctacgct catcctgccc cgcaaatcca ccggccgaag
    13561 cccgcttcaa ctcaccgcgc aggcggcgcc gatccgccgc caggcgagcg gcccagccga
    13621 acagggacag cacgccgagc agccagccga gggccagggc ggcgacgaag gccagcccca
    13681 ggggcgcctt gagcgctgcg aaaccgaggt cgaggttgat ttcgcccgga ttgagcgaag
    13741 tgaagaaggc cgacagcgcg agcgcggtaa ggaagatgac cgccgtgaaa atccgcttga
    13801 ccatgatgat cctgccaaca gcgcgccgcg gcgcgattca gcgcggattg tatcagcgag
    13861 aacgggcccg ggtggccctg cggccgtcag gccttgatgg gatggtcgcg cccctcgttg
    13921 acccgctcgc gcaggtcctt gccgggcttg aagtgcggaa cgtacttccc ggacagcgcc
    13981 accgcttcgc ccgtcttcgg gttgcggccg atgcgcggcg ggcggaagtg cagggagaaa
    14041 ctgccgaaac cgcgaatctc gatgcgctcg ccgctggcca ggctgttgct catgacctcg
    14101 atcaggtact tgaccgccag ttcgacgtcc ttggcgttca agtggctctg gcgacgggca
    14161 attgcctcga tcagttcaga ctttgtcatg ttcttgttcc gtcagcggcc ccaaaccgat
    14221 gttccccgca gcgccggaaa cggcgggccg ccagccggcg gcccgcctcc ggtgtcctca
    14281 ggaattctcc tggctcgaga tctgctcctt gagcaggtcg ccgagactcg tcccggtagt
    14341 cgtgtccgac ttcttgtagc tctgcatggc ctcggcctct tcccagactt ccttggcctt
    14401 gatggacagc gaaatctgcc ggctcttccg atccaggccg atgaacctgg cctcgatctc
    14461 ctcgccctgc ttgagcaccg cacgcgcatc ctcgacccgc tcacgggaaa gctcggaagc
    14521 ccgcaggtag ccctcgaggc cgcccacgtc gacgatcgcg cccttggcat cgacctcggt
    14581 gaccacaccc ttgacgatgc tgcccttcgg gttctccgcc agccaggccg acatcgggtc
    14641 gcgatccatc tgcttcacgc cgagggaaat gcgctcgcgc tccgggtcga tcgacagcac
    14701 cacggcttcg acctcctcac ccttgcggta attgcggacc gcttcctcgc ccggcaggtc
    14761 ccaggaaagg tcggagaggt gcaccagtcc gtcgatgccg ccgtccagcc cgatgaagat
    14821 gccgaaatcg gtgatcgact tgatgacgcc cttgacccgg tcacccttgt tgaagctcgt
    14881 ggcgaattcg ttccaggggt tcggcatgca ctgcttgata ccgagggaca cgcgacggcg
    14941 ctcctcgtcg atctccagca ccatgacctc gatctcgtcg ccgatctgca cgatcttgga
    15001 cgggttgacg ttcttgttcg tccagtccat ctcggagacg tgcaccaggc cctcgacgcc
    15061 gtcctcgatc tccacgaagg cgccgtaatc ggccaggttc gtaaccttgc cgaacaggcg
    15121 ggtgccggtc gggtagcgac gcgcgatgtc ctgccagggg tcctcgccca gctgcttgag
    15181 ccccagcgat acgcggttgc gctcgcggtc gaacttgagc accttgacct ggatctcgtc
    15241 gccgacgttg acgacttccg agggattctt gacgcggcgc caggccatgt cggtgatgtg
    15301 caacaggccg tcgatgccgc ccaggtccac gaaggcgccg tagtcggtca ggttcttgac
    15361 gatgcccggg agcacggcgc cctcggtgag gttcttcatc agctcctcgc gctcgacgct
    15421 gctctcggcc tcgaccaccg cgcgacgcga aaccaccacg ttgttgcgct tgcggtcgag
    15481 cttgatgacc ttgaactcga gcatcttgtt ctcgagatag ctggggtcgc gcaccggcct
    15541 gacgtccacc agggagcccg gcaggaaggc gcgcaccagt tcgagctcga cggtgaagcc
    15601 gcccttgacc cgcccggtga tcatgccctg cacgatctcg cccttctcga aggcttcctc
    15661 caggacggtc caggtacgcg agcgcttggc cttgtcgcgg gacaggcggg tttccccgaa
    15721 gccgtcctcg acggcgtcca gcgcgacttc gacctcgtcg ccgacggcga cttcccactc
    15781 gcctctctcg ttcttgaact gctcggcggg aatgacggac tcagacttga gtccagccga
    15841 cacgatgacg acgtcatcgt tgatctcgac cacgcggccc atgaggatgg cgccggggcg
    15901 aatctgctga ctggcaaaac tttgctcgaa gagctctgcg aaactttctg acatg
//
LOCUS       DAOMPG010000029        15838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1608_length_15838_cov_3.902300, whole genome shotgun sequence.
ACCESSION   DAOMPG010000029 DAOMPG010000000
VERSION     DAOMPG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15838
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_15838_cov_3.902300"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLS34_02705"
     CDS             complement(<1..1224)
                     /locus_tag="PLS34_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRP86417.1"
                     /translation="MRRSFRVPHTLVLLFGMMVAALVATWLLPSGEFETVVNDSGRAV
                     VVPGTYAVLADARRLMPWELFTVIPRALADAQDIIFFVLIVGGAIRVLRATGTIDAML
                     GTVLRRIGHSPGLLIGIGMGVFAAGSATLGVAEEYIPLTLVLISLCVAMRMDAVTAIG
                     IMVCGYGIGYGVAALNPFTVLVAQRVADLPPASGLGFRLAIFLPFLAIGIHHVWSYAR
                     RVQADPQASLVHDIEGAQHEAAEMHPGMSGRHKAVVVLTLAALGAMVYGIAVPKWYIL
                     ELGAIFLGLALVGGLVGGLGANDTAKRFAEGAAELAGTALLIGFARSIALILEDGQVL
                     HTIVNGLAAPLQVMGAEFAAIGMLAIQTLINLFIPSGSGQAYVTMPLMAPIGDLVGLT
                     RQTAVLAFQFGDGFAN"
     gene            1365..2921
                     /locus_tag="PLS34_02710"
     CDS             1365..2921
                     /locus_tag="PLS34_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005491083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRP86418.1"
                     /translation="MAGAREITETTTPNPRIARILDWIERIGNKLPDPAVLFLLALVI
                     TWIVSALLAPVSFSAIDPRTGEAVVVKNLLAGQELTGFLSQMVQVFVAFPPLGVVLVA
                     MLGIGVAEHTGFINAGLRGLLSFTHRGLLTPMIITVGILSHTAVDAGYVLVVPLGGVI
                     FYAAGRHPLAGIAAAFAGVSGGFSANFVPSSVDPMLAAITQVGGQVLDQALLINPLNN
                     YFFTAISSLLIIGLGWYLTDKIVEPRLQKTALDGDLKDLPTMEALEPAERRALRWSLL
                     SMVIALALLVVTAMPAASPWRDPAGELATSAAPLMRSIVPLIFLLFVIPGMVYGYLSG
                     TVKSHRDIVQGMSKAMSGMGYYVVMAFFAAQFIFAFGQSNLGALLAIEGANFLRALGM
                     PAQVTLIGIILVTGLVNLVVGSASAKWALLAPILVPMLMSLGISPDLTQAAYRVGDST
                     TNIITPLMPYFPLIVVFCQRYVTNAGIGTLVAMMLPYSVVFMVAWSAFLLLYWTLGLP
                     LGLQSSYVYP"
     gene            complement(2972..3760)
                     /locus_tag="PLS34_02715"
     CDS             complement(2972..3760)
                     /locus_tag="PLS34_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRP86419.1"
                     /translation="MSSCKSVVALAGLCWPLVVGAWGHDGHRAAAAVTADRLCPAAAV
                     AVERLLDGMTLAESSTWPDGIRRQAEWAHTRDWHYINIGDEQPMSTLVEGAPGHGRLL
                     SAIRENLALLGNERADATRRRQALSFVLHLVADVHQPLHVGRLEDRGGNAVTVSFEGG
                     ETSLHRLWDSGLLRSSGLRATDYRRSLAPLVMIGATSWERGSLEDWATESQRLRPWVY
                     DFDTRRRVPHISKRYAETGRQLTALRLAQAGVRTAWLLNQVWCD"
     gene            complement(3757..4434)
                     /locus_tag="PLS34_02720"
     CDS             complement(3757..4434)
                     /locus_tag="PLS34_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP86420.1"
                     /translation="MRTVLYSLLLLASFAGPTTAANPASALASAEITVREALQSLSGM
                     VSDCRLQLAADPVALRGIIDHHLRPRADVLYAGQLILGRHWSEAEPEQRRRFAEALYG
                     TLANRYASGLLLLTGRNVAVVGSNETGDQGPVQVELRIQTGLASPLPVFLQLRLGGER
                     WRIFDARWEGQSYVLSLRHAVSQQIRRDGIEAVIRRLEATAGAPAGPPEERQTAAGRC
                     LGARARS"
     gene            4575..6992
                     /locus_tag="PLS34_02725"
     CDS             4575..6992
                     /locus_tag="PLS34_02725"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRP86421.1"
                     /translation="MNIPQSTLPEPLGFDDASLRHDTLKELLYGVGKDVDTATLHDLY
                     SAVALAIRDRLVGRWHETQRRIRHNGPRKVYYLSLEFLMGRTLLNAAINLDLDASARK
                     LLEEIGVRLERLVEEEQDAGLGNGGLGRLAACFLDSMATLDLAGFGYGIRYDYGLFEQ
                     RISPDGQQIELPNTWLRFRNLWEIKREDIRYEVRIGGQSIEAVAYDTPVPGNNGRTVN
                     HLRLWGARYAPEIDLALFNAGDYEAAVRAKNEAENISRVLYPDDSTEEGKELRIRQEF
                     FFVSASLQDILHEHRAAGLPWSGLPSRVAIQLNDTHPALAVPELMRLLADEHGLDWDE
                     AWSITRGVFSYTNHTLLPEALETWPVRMLEKLVPRHLEIIYRINREFLDEVWQRHPGD
                     NERRRRMSIVDEAGHDGRRVRMAWLATVGSRKVNGVAALHSDLVRRTLFRDFDEYYPG
                     RFVNVTNGVTPRRWMRAANPRLSALLDERLGRGWANDLHRLRELAQLADDTDFRQRLR
                     EIKHANKERLARHIQRETGVVVSPDSLFDIQIKRIHEYKRQLLNLLHVITRYNRIRQD
                     PERDWLPRTVVFAGKAAPAYTMAKRIIQLINNVAETVNGDWAVGDRLKVVFLPNYSVS
                     AAELIIPAADLSEQISTAGMEASGTGNMKFAMNGALTIGTLDGANIEIRDAVGEENIF
                     IFGLTADEVAARRAHGYYPIELARNNAELGEVLRQVRDGFFCPAELRRFAPVVDALIE
                     DGEHFLCLADYEHYVDTQERVDGEYRDADGWMRKAAINISAMGRFSSDRAIDEYARRI
                     WESGPIG"
     gene            6996..9191
                     /gene="glgB"
                     /locus_tag="PLS34_02730"
     CDS             6996..9191
                     /gene="glgB"
                     /locus_tag="PLS34_02730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRP86422.1"
                     /translation="MEDAATIHLLSELHQARCAVPFQFLGLHESPDGKGMVLRAWRPA
                     VDWVELIDLEARHNLGRLDRVGDGDLFIKRLPRRRHAFAYRLRLGVGARVLEDDDPYQ
                     FRRAVFARGSEDRNRLYRWFGAHAETLESDSGRAVEGVRFMVHAPAARSVSVVGDFND
                     WDGRRHPMQSSYEGDWRLFVPGLKPGTLYKYEIKGPDGRLLPLKADPFGVYHEQPPGN
                     ASIVAAPAGHSWSDADWINARREAGYRNNQPLAIYELHAGSWRRRDGRPLSYRELADE
                     LVPYLRDTGFTHVEFMPLAEHPFAASWGYQPTGMFAASSRFGPPDDLRFLVDRLHAAG
                     IGVILDWVPGHFPADPHGLGRFDGTPLYEHHDPRRGWHPDWDTHVYDFGSAWVKDFLV
                     SSAMYWVEQFHVDGLRVDAVASMLYLDYSRQAGQWLPNMFGGNQNLEAIDFIKRFNEV
                     IHAEHPGVLTIAEESTAWPGVSRPTYDGGLGFGFKWNMGWMHDTLKYMARDPVHRRFH
                     HGDLTFGLIYAWDENFVLPLSHDEVVHGKGSLLNKMPGDDWQKLANLRLYLAFMYAHP
                     GKKLLFMGAELAQPAEWNHDAELAWGLLDEPSHRGVHELVRDLNRIYRDQAAFWEADH
                     SPDGFEWIDYSDADHGVLAFLRHDAARARHVLCVCNFTPVVQHGYRLGVPRVGAYREV
                     LNTDSALYGGSNAGNQGRVEVGAVPSHGRGQSVELTLPPLAVLYLVPEA"
     gene            9197..11317
                     /gene="glgX"
                     /locus_tag="PLS34_02735"
     CDS             9197..11317
                     /gene="glgX"
                     /locus_tag="PLS34_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRP86423.1"
                     /translation="MLRSAPAMLAGRATPLGATWTGDGVNFALFSAHAEAVDLCLFDA
                     LGQRELARLPLAQRSGDTWHGFLPGAGPGLVYGYRVHGRYAPRQGHRFNKHKLVLDPY
                     ARALVGELRYGDEIYGFSRGAAEAWRQDISDSAGRVPKARVVDAHAFDWRGDLPPRTP
                     MDRSVIYELHTRGFTRLHPGVPPHLRGTWLGLAQPAVLDYLVGLGVTAVELMPVQQFV
                     TEPHLHARGLSNYWGYNTLAFFAPHAGYALADPLREFREMVRAMHGAGLEVIIDVVFN
                     HTAEGGDGGPTLSMRGIDNLSYYRLVPDDLRHYLNWTGCGNTLNMDHPAVLRLVIDCL
                     RYWVAEMHVDGFRFDLATTLGREGETFHRDGRFFTAIREDPLLAGVKLIAEPWDLGPD
                     GYRLGSFPPEWAEWNDRFRDTVRSYWRGDPGVVPQLAERLAGSSDLFASGGRGPPESV
                     NFVACHDGFTLRDTVSYAVRHNEANGENGQDGDHHAVSWNNGVEGTTEDRKVVALRYR
                     QQRNLLATLLLSQGVPMLQAGDEFGRTQRGNNNAYCQDNETSWVDWRLARSNSALLEF
                     VRHLLRIRADNAVFRRQGFLAGVQREAGRFKDVVWLSSDGQELAEPDWVDPGRQAFAM
                     LLDSTGLPPTQLMPDVGDSFLVLFNAAPEAVEFTLPAPLSNAVWEVVLDTSQEALTVP
                     AEGYRQGHGYLLADRSLALLVDHG"
     gene            11310..13460
                     /gene="malQ"
                     /locus_tag="PLS34_02740"
     CDS             11310..13460
                     /gene="malQ"
                     /locus_tag="PLS34_02740"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRP86424.1"
                     /translation="MADRGELLDRLRRLRGIAGSFTDYRGETHELGESSLLGLLAALG
                     HTVDNPAALVREAEALETRDWIRVLGPVEVLRGSRTVPFTVLAPLLPAIRWRVTTESG
                     DTLDGEIVPAALPVLEERGIGALWYVRLALELPQLPAGYHRLWLEKPDGQPLGNTRLI
                     VAPERCFEPIVIRDGGRPWGLAVQLYALRSGRNWGIGDFTDLAGFAAAAGGLGADLIG
                     LNPLHALFPADPALCAPYSPSSRYFLNALYIDPEAIPEFRDSVEAQRLVGTPEFQARL
                     EALRAAPFVDYPGVTACKLEVLRTLHSEFATRASTARRHDFNEFLKKHGQVLERFAVF
                     HALHEQFVARGERGGWPEWPAGYQRPGEPAVQAFAEVEPAAVDFHCWLQWTAASQLAA
                     AEQCARAAGLRLGLYRDLAVGPNGGGAETWTARELYARGATVGAPPDPLALQGQDWGI
                     PPFDPDALRERAYAPFIRLLRANMGQDGALRIDHVMMLMRLWWVPRGMPSSAGGYVHY
                     RLDEFMGIVALESQRRRCLVVGEDLGTVPPEVRTAMSAHGLYSYRVLLFEREADGRFR
                     APADYPREALVTPSTHDLPTLASFWAGTDIELRQRLALYPEPGLAEEQRRARQDAREA
                     LLAALRDAGLQAVATNLPPTCEVQRFLAMAPAAVLMLQPEDWLSMETPVNVPGTHTEY
                     PNWGRKLSADWPEFMQRADLRAFAADISRLRQSG"
     gene            13507..>15838
                     /locus_tag="PLS34_02745"
     CDS             13507..>15838
                     /locus_tag="PLS34_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRP86425.1"
                     /translation="MTDFAEILAGTEAVERVPAKALAEALASGALIVTATRRLARRLQ
                     AEHARAMREPGWDTPAVVPWSAWLQSTYADLRDFGALATPQPCLDDWQSAALWEEAFH
                     ADPVAGTLLMAGGAVDGFRDAWRLVQEWRLPWPELQKRAGEDCGVFLRLAGRYRGRLQ
                     ALDCIDRAGLPAVLATALQDRSGPDVFFAGFDALHPAQALLIRSLGRRAKLAAPPHHA
                     AAPTRAAYPDSRRELAAAAAWARRRLEQDPGARLGIVVPDLDAQASLLEDLLDEALVP
                     ARLLPGRSDVPRPWNLSLGRPLADAPIVAAALLALGSTSRDGIETAEASRLLRSPFFG
                     GAAAEAGHRARLEAWLRGHAPDRIPIAALPGWLAGRAGAPECPQFADGIGACLEEVRR
                     GPRRQRPSDWAAAMTRALARLGWPGDTPLDSEAWQTVQAWSELLEDFSRLDVVAGPLT
                     FADALTKVRRMGVERRFQPETPEVPVQVLGLLETAGLEFDGLWVTGMHDGALPAPLRP
                     CALLPAALQRELGMPRACPDTELALARRMVTRLSGAAREVRFSHPATRQDEPLRPSPI
                     IAALPAATDSDDFTRVGAAARAFATRRLESRPDDTGPPVRGEVGGGTGLLAAQSACPF
                     RAFAMHRLAAHELARPEAGIDGLTRGSFMHEALRFLWGELRDQAGLASLDPPARMARV
                     RAAVERAASTALAGLPPGLVGIELAEAVRRISELLEVELLRPPFEVLQREERVAIEIG
                     PLRINGQVDRIDRTTHGVVIIDYKTGRVPSPKELEGL"
BASE COUNT         2475 a         5406 c         5456 g         2501 t
ORIGIN      
        1 gttggcgaag ccgtcgccga actggaaggc cagcacggcc gtctggcgcg tcaagccgac
       61 caggtcgccg atcggcgcca tcagcggcat ggtgacgtag gcctggccag agcccgacgg
      121 gatgaacagg ttgatcagcg tctggatggc cagcatgccg atggcggcga actctgcgcc
      181 catcacctgc aggggtgccg ccaggccgtt gacaatggtg tgcaggacct ggccgtcctc
      241 caggatcagg gcgatcgagc gggcgaaacc gatcagcaag gccgtgccgg ccagttcggc
      301 cgcgccctcc gcgaagcgct ttgccgtgtc gttcgcccca agcccgccga ccaggccacc
      361 gaccagtgcc agcccgagga agatggcgcc gagttccagg atgtaccact tcggcacggc
      421 gatgccgtag accatggcgc cgagcgcggc gagggtcagc acgacgaccg ccttgtgccg
      481 gccgctcatg ccgggatgca tctcggccgc ctcgtgctgg gctccttcga tgtcgtgcac
      541 caggctcgcc tgcggatcgg cctgcacgcg gcgcgcgtaa ctccagacgt ggtggatgcc
      601 gatcgccagg aagggcagga agatcgccag cctgaagcca agtccggagg cgggcggcag
      661 gtccgcgacc cgctgcgcca ccagcacggt gaacgggttg agtgccgcca cgccgtagcc
      721 gatgccgtag ccgcatacca tgatgccgat ggcggtgacg gcgtccatgc gcattgcaac
      781 gcacaacgag atcagcacca gggtcagggg gatatattcc tcggccacgc ccagcgtggc
      841 ggagccggct gcgaacacac ccatgccgat gccgatcagc agccccgggc tgtggccgat
      901 gcggcgcagc acggtgccga gcatcgcgtc gatggtgccg gtggcgcgca gcacccggat
      961 ggccccgccg acgatcagca cgaaaaaaat gatgtcctgc gcgtcagcca gcgcgcgggg
     1021 aatcaccgtg aacaattccc agggcatcag tcgccgggca tcggcaagga ccgcgtaagt
     1081 ccccggtacg acgacggcgc gtccgctgtc gttgacgact gtttcgaatt ccccggacgg
     1141 cagcagccag gtggctacca gggcggccac catcatgccg aacagcagca ccagcgtgtg
     1201 gggaacgcga aaagagcgac gcatgccggt gagctcccaa agtgaagaag tcaaagtggc
     1261 gccagatgct gaccgatcgg ccgcccggag gcaaacggcg cgtcccggct ggtcttgctg
     1321 cgccacgcgg ctatactgcc gccttcgcgc aatggaggca gccgatggcc ggggcacgag
     1381 aaatcacaga aaccaccacc cccaacccgc gcattgcgag gatcctcgac tggatcgagc
     1441 ggatcggcaa caagctgccg gacccggcgg tgctgttcct gctggcgctg gtcatcacct
     1501 ggatcgtgtc ggcgctgctg gcgccggtga gcttctcggc gatcgacccg cgcaccggcg
     1561 aagcggtggt agtgaagaac ctgctggccg gccaggaact gacgggcttc ctgtcgcaga
     1621 tggtgcaggt gttcgtcgcc ttcccaccgc tcggcgtcgt gctggtggcg atgctcggca
     1681 tcggcgtcgc cgagcacacg ggcttcatca atgcaggctt gcgcgggctg ctgtcgttca
     1741 cgcaccgggg gctgctgacg ccgatgatca tcacggtcgg catcctcagc cacaccgccg
     1801 tggatgcagg ctacgtgctc gtcgtgccgc tgggcggcgt cattttctac gcggcgggcc
     1861 gccacccgct ggcaggcatc gccgcggcct tcgccggtgt atcgggcggc ttttccgcca
     1921 atttcgtgcc ctcgagcgtc gatccgatgc tggccgccat cacccaggtg ggcggacagg
     1981 tgctcgacca ggcactgttg atcaatccgc tcaacaacta tttcttcact gcgatttcat
     2041 cgctgctgat catcggcctc ggctggtacc tgacggacaa gatcgtcgag ccccggctgc
     2101 agaaaactgc actggacggc gacctgaagg acctgcccac catggaggcg ctggagccgg
     2161 ccgagcggcg cgcgctgcgc tggtcgctgc tgtccatggt gatcgcgctg gcattgctgg
     2221 tcgtgacggc catgccggcc gcttcgccct ggcgcgaccc ggccggcgag ctggccacct
     2281 cggcggcgcc gctgatgcgc tcgatcgtgc cgctgatctt cctgctgttc gtcatccccg
     2341 gcatggtgta cggctacctg tccggcaccg tgaaatcgca tcgtgacatc gtccagggta
     2401 tgtccaaggc gatgagcggc atgggctatt acgtggtaat ggccttcttc gccgcgcagt
     2461 tcatcttcgc gttcggacaa tcgaacctcg gcgccctgct ggccatcgag ggcgcgaact
     2521 tcctccgcgc gctcggcatg cccgcacagg tgacgctgat cggcataatc ctggtgaccg
     2581 ggctcgtcaa cctggtggtg ggctcggcgt ccgccaagtg ggcgctgctg gcgccgatcc
     2641 tcgtgccgat gctgatgagt ctcggcatct cgccggatct gacccaggcg gcctaccgcg
     2701 tcggcgactc gaccaccaac atcatcaccc cgctgatgcc gtactttccg ctgatcgtag
     2761 tgttctgcca gcgttacgtc accaatgccg gcatcggcac gctggtcgcg atgatgctgc
     2821 cgtactcggt ggtgttcatg gtggcctggt cggcgttcct gctgctgtac tggacgctgg
     2881 gcctgccgct cggcctgcaa tccagctacg tctatccctg aaattgggga cattccccaa
     2941 ttccagacgg aattggggaa tgtccccaat ttcagtcgca ccacacctgg tttagcagcc
     3001 aggcggtgcg gacacccgcc tgggcaagcc gcagtgcggt cagctgccgg cccgtttccg
     3061 catagcgctt cgaaatatgc ggcacgcgcc ggcgcgtgtc gaagtcgtac acccaggggc
     3121 gcaggcgctg cgactccgtg gcccagtcct ccagcgagcc ccgttcccat gaggtcgcgc
     3181 cgatcatcac cagcggcgcg aggctgcgcc gatagtccgt cgcgcgcagg cccgagctgc
     3241 gcaacaggcc gctgtcccag aggcgatgca ggctggtctc gccgccctcg aagctcacgg
     3301 tgaccgcgtt gccgccgcgg tcctcgagcc gaccgacgtg cagcggctgg tggacgtcgg
     3361 cgaccaggtg cagcacgaag ctgagtgcct ggcgacggcg cgtcgcgtca gcacgctcat
     3421 tgccaagcag ggcgaggttc tcgcggatgg cggacagcag gcgaccatgc cccggcgcgc
     3481 cttcgaccag tgtgctcatc ggctgctcat cgccgatatt gatgtagtgc cagtcgcgtg
     3541 tgtgcgccca ctccgcctgc ctgcgaatgc catcaggcca ggtggacgac tcggccaggg
     3601 tcataccgtc cagcaaccgc tcgaccgcga cggccgcggc gggacagagc ctgtccgcag
     3661 tcacggcggc ggcggcgcga tgaccgtcat gcccccatgc gccgactacc agcggccagc
     3721 acaggccggc cagggcgacc acggacttgc agctgctcat gaccgtgctc gcgcgcccag
     3781 gcatcggccc gcggcggtct ggcgctcctc cggagggccg gcgggggcac cggctgtggc
     3841 ttcgagtcga cggatcaccg cctcgattcc gtcgcgccgg atctgttgcg agacggcatg
     3901 gcgcaggctc agcacgtagg actgcccctc ccagcgcgca tcgaaaatgc gccagcgctc
     3961 cccgcccagc cgcaactgca agaacaccgg caggggcgaa gccagcccgg tctggatccg
     4021 cagctccacc tggactggac cctggtctcc ggtctcgttc gagcccacca ccgccacgtt
     4081 gcgtccggtc agcaacagca ggccggacgc atagcggttg gccagcgttc catagagcgc
     4141 ctcggcaaag cgacgtcgct gttcgggctc agcctccgac cagtgtcgcc cgaggatcag
     4201 ctgccctgcg tacagcacgt cggccctcgg ccggaggtgg tggtcgatga tgccgcgcaa
     4261 cgccaccggg tcggccgcca gctgcaggcg gcagtcggac accatgccgg aaagcgactg
     4321 cagcgcttca cggaccgtga tctccgccga cgccagtgcc gacgccgggt tggcggcggt
     4381 ggtgggcccg gcgaaagacg ccagtagcag caggctgtac agaacggtgc gcatttggcc
     4441 ccacagtatc cgggccggtc gcccacgggg caaacctcgg cggcgtgatt tggtgctcag
     4501 caacgggcgg cacgtctgtt atgctccggc gcgaaccagc gggtcgccga tgtcggatcc
     4561 aggagccaca ggcattgaat atcccccaga gcaccttgcc cgagccattg ggtttcgatg
     4621 acgcgagcct gcgacacgat acgctgaaag aactgctcta cggggtcggc aaggacgtcg
     4681 acacggcgac gctgcacgac ctgtacagcg cggtcgccct ggcaatccgc gaccggctgg
     4741 tcggccggtg gcacgagacg caacggcgca tccgccataa cgggccccgc aaggtgtatt
     4801 acctgtcact cgagttcctg atgggacgca ccctgctcaa tgcggcgatc aacctggatc
     4861 tcgacgccag cgcccgcaag ctgctcgagg aaatcggcgt ccggctcgag cgcctggtag
     4921 aagaagagca ggatgccggg ctcggcaatg gcggcctcgg gcgcctggca gcgtgctttc
     4981 tcgactccat ggcgacgctc gacctggcgg gcttcgggta cggcatccgt tacgactacg
     5041 ggctgttcga gcaacggatc tcgcccgacg ggcagcagat agaactgccc aacacctggc
     5101 tgcgcttccg caacctgtgg gagatcaagc gagaggacat ccgctacgag gtccgcatcg
     5161 gcggccagag catcgaggcg gtggcctacg acacaccggt gccgggcaac aatggccgga
     5221 ccgtgaacca cctgcgtctc tggggtgcgc gctacgctcc ggaaatcgac ctggccctgt
     5281 tcaatgccgg cgactacgag gccgcggtgc gcgccaagaa cgaggccgag aacatttcgc
     5341 gcgtgctgta ccccgacgac tcgacggagg agggcaagga actccgcatc aggcaggaat
     5401 tcttcttcgt gtccgccagc ctgcaggaca ttctccacga gcatcgcgcc gccgggctgc
     5461 catggtcggg gttgccatcg cgcgtcgcga tccagctcaa cgacacccat ccggcgctgg
     5521 cggttcccga gctcatgcgg ctgctcgccg acgagcatgg cctggactgg gacgaggcct
     5581 ggtcaatcac gcgcggggtc ttcagctaca ccaatcacac gctcctgccc gaggccctgg
     5641 agacctggcc ggtacgcatg ctggagaagc tggtgccgcg gcacctcgag atcatctacc
     5701 gcatcaatcg cgagttcctt gatgaggtct ggcagcgaca cccgggcgac aacgagcgcc
     5761 ggcggcgcat gtccatcgtg gacgaggcgg gccacgacgg gcgacgggtc cgcatggcct
     5821 ggctggccac cgtagggtcg cgcaaggtca acggcgtcgc cgcgctgcac tcggacctgg
     5881 tgcgccggac cctgttccgt gatttcgacg agtattaccc ggggcgattc gtcaacgtca
     5941 ccaacggcgt cacgccgcgt cgctggatgc gcgccgccaa tccgcggctg tcggcgctgc
     6001 tggacgagcg gctcggccgg ggctgggcga acgacctgca ccggctccgc gagcttgcgc
     6061 aactggcgga cgatacggat ttccggcaac gcctgcgcga gatcaagcat gccaacaagg
     6121 agcgcctggc ccgccacatc cagcgcgaga caggcgtcgt ggtcagcccc gactcgctgt
     6181 tcgacatcca gatcaagcgc atccacgagt acaagcgaca gctgctcaac ctgctgcacg
     6241 tcatcacgcg ctacaaccgc atccgccagg atcccgagcg cgactggctg ccccgcaccg
     6301 tggtgttcgc cggcaaggcc gcaccggcct acaccatggc caagcgcatc atccagctga
     6361 tcaacaacgt cgccgagacc gtcaacggcg actgggcggt gggcgaccgg ctgaaggtcg
     6421 tgttcctgcc caactacagc gtctccgccg ccgagctcat catccccgcc gccgatctgt
     6481 ccgagcagat ctccacggcc ggcatggaag cctcgggcac ggggaacatg aaattcgcga
     6541 tgaacggcgc gcttaccata ggcactctcg atggcgccaa tatcgagatt cgcgacgcgg
     6601 tcggggagga gaacatcttc attttcgggc tcaccgcgga cgaggtggct gcgcgtcgcg
     6661 cgcacggcta ctacccgatc gagctggcgc gcaacaatgc cgagctcggc gaggtgctgc
     6721 gccaggtccg cgacggtttc ttctgcccgg ccgagctgcg tcgttttgcg cccgtggtgg
     6781 acgcgctgat cgaggacggc gagcatttcc tgtgcctggc ggattacgag cactacgtgg
     6841 acacccagga gcgcgtcgat ggggagtacc gcgacgccga cggctggatg cgcaaggccg
     6901 ccatcaacat ctccgcaatg gggcgcttct cgagcgaccg cgccatcgat gagtacgcgc
     6961 ggcgtatctg ggagtcgggg ccgatcggct agcccatgga agatgcggcc acgatccacc
     7021 tgttgagcga gctgcaccag gcacgctgcg cggtgccgtt ccagttcctt ggcctgcacg
     7081 aatcgccgga tggcaaggga atggtactca gggcctggcg gccggcggtc gactgggtcg
     7141 aactcatcga cctcgaggca cgtcacaacc tgggccgcct cgaccgagtc ggtgacggcg
     7201 acctgttcat caagcgattg ccgcgccgcc gccatgcgtt cgcctatcgg ctgcgcctcg
     7261 gcgtcggcgc gcgcgtactc gaggacgacg atccctacca gttccgccgc gcggtgttcg
     7321 cccgggggtc agaggaccgc aaccggctgt atcgctggtt cggcgcgcat gccgagacgc
     7381 tggagagcga cagcggcagg gccgtcgagg gggtgcgttt catggtccac gcgccggcgg
     7441 cgcgcagcgt cagcgtggtc ggcgatttca acgactggga cgggcgccgc catcccatgc
     7501 agagctctta cgagggtgac tggcggctgt tcgtccccgg gctcaagccg gggacgctct
     7561 acaagtacga gatcaagggt ccggacggtc gcctgctgcc gttgaaggcc gatcctttcg
     7621 gcgtctacca cgagcagccc ccgggcaacg catcgatcgt cgcggcgccc gccgggcaca
     7681 gctggagtga tgcggactgg atcaacgcac gccgcgaggc cggctatcgc aacaatcagc
     7741 cgctggccat ctacgaactg catgccggct cctggcgccg ccgcgacggc cggccgctgt
     7801 cctatcgcga gctggccgac gaactggtgc cctacctgcg ggacacgggc ttcacgcatg
     7861 tcgagttcat gccgctggca gagcatccct ttgccgcttc ctggggctat cagccgaccg
     7921 gcatgttcgc ggcgtccagt cgcttcgggc cgccggacga cctgcgattc ctggtggacc
     7981 gcctgcatgc ggcgggcatc ggcgtcattc tcgactgggt gcccgggcat tttcccgccg
     8041 atccgcacgg cctcgggcgc ttcgacggca cgccgctgta cgagcaccac gacccgcggc
     8101 gcggctggca tccggactgg gatacccacg tgtacgattt cggcagcgcc tgggtgaagg
     8161 atttcctggt gtccagcgcc atgtactggg tcgagcagtt ccacgtcgat ggcctgcgcg
     8221 tcgacgccgt cgcgtcgatg ctgtacctgg attactcccg gcaggccggc cagtggctgc
     8281 ccaacatgtt cggcggtaac cagaacctcg aggccatcga tttcatcaag cgcttcaacg
     8341 aggtcatcca cgccgagcac ccgggcgtgc tgaccatcgc cgaagagtcc accgcctggc
     8401 ctggcgtgtc gcggccgacc tacgacggcg gcctcggctt cggcttcaag tggaacatgg
     8461 gctggatgca cgacaccctg aaatacatgg ccagggaccc ggtacaccgg cgcttccacc
     8521 acggcgacct caccttcggg ctcatctacg cctgggacga gaacttcgtg ctgccgctct
     8581 cacacgacga agtcgtgcac ggcaagggtt cgctgctaaa caagatgccc ggcgacgact
     8641 ggcagaagct ggccaacctg cgcctgtacc tggccttcat gtacgcccat ccgggcaaga
     8701 agctgttgtt catgggcgcc gaactggcgc agcccgcgga atggaaccac gatgccgagc
     8761 tggcctgggg cctgctcgat gagccgtcac accgtggcgt gcacgagctg gtgcgtgatc
     8821 tcaaccgcat ctatcgagat caggctgcct tctgggaggc cgaccatagc ccggacggct
     8881 tcgagtggat cgattacagc gacgccgacc acggggtgct ggcgttcctg aggcatgacg
     8941 cggcgcgcgc acgccatgtg ctctgcgtgt gcaacttcac gccggtggtg cagcacggct
     9001 accggctcgg cgtgccgcgc gttggagctt atcgcgaggt gctgaacacc gactcagccc
     9061 tgtacggcgg cagcaacgcc ggcaaccagg gccgggtcga ggtgggcgca gttccgagcc
     9121 acgggcgcgg ccagtcagtg gagctgacgc tgccgccgct ggccgtgctc tacctggtgc
     9181 cggaggcatg agcccgatgc tgaggagtgc gcccgccatg ctggcgggac gggccacgcc
     9241 gctgggcgcg acctggacgg gcgacggcgt gaacttcgcg ctgttctcgg cacacgccga
     9301 agccgtcgac ctgtgcctgt tcgacgccct cgggcagcgc gagctggcgc gcctgccgct
     9361 ggcgcaacgc agcggcgaca cctggcacgg cttcctgccg ggcgcaggcc cgggcctcgt
     9421 ctacggttac cgggtgcacg gccgctatgc gccgcgccag ggccatcgct tcaacaagca
     9481 caagctcgtg ctcgacccct acgcacgcgc cctggtgggc gagttgcgct acggcgacga
     9541 gatctacggc ttcagccgcg gcgccgccga agcgtggcgc caggacatca gcgacagcgc
     9601 gggccgggtg cccaaggccc gggtcgtgga cgcgcacgcc ttcgactggc gcggcgacct
     9661 gccgccgcgc acgcccatgg accggagcgt catctacgaa ctgcacacca ggggcttcac
     9721 gcgcctgcat cccggcgtgc cgccgcacct gcgcggcacc tggctcgggc tggcccagcc
     9781 cgcggtgctc gactacctgg tcggcctcgg cgtcactgcc gtcgaactga tgccggtaca
     9841 gcagttcgtc accgagcctc acctgcatgc gcgtggactg agcaactact ggggctacaa
     9901 cacgctggct tttttcgcgc cgcacgccgg ctacgcactg gcggacccgc tgcgcgagtt
     9961 ccgcgaaatg gtgcgggcca tgcacggcgc cggtctcgag gtgatcatcg acgtcgtgtt
    10021 caaccatacg gcggagggcg gcgacggcgg cccgaccctg tccatgcgcg gcatcgacaa
    10081 cctgagctac taccgcctgg tgccggacga cctgcgtcac tacctgaact ggacgggctg
    10141 cggcaacacc ctgaacatgg accatccggc ggtgctgcgc ctggtcatcg actgcctgcg
    10201 ctactgggtg gccgagatgc acgtggacgg cttccgcttc gacctcgcga ccacgctggg
    10261 cagggaaggc gagaccttcc atcgcgacgg ccgcttcttc accgccattc gtgaggaccc
    10321 gctgctggcg ggcgtgaagc tcatcgcgga gccgtgggac ctcggccccg acggctaccg
    10381 gctgggcagt ttcccgccgg aatgggccga gtggaacgac cgcttccgcg acaccgtgcg
    10441 cagctactgg cgcggggatc cgggcgtcgt gccgcagctg gcggaacggc tggccggctc
    10501 cagcgacctg ttcgcctcgg gtggccgcgg gccgccggaa agtgtcaatt tcgtcgcctg
    10561 ccacgacggt ttcacgctac gcgataccgt aagctacgcg gtcaggcaca acgaggccaa
    10621 cggcgagaac ggccaggacg gtgaccatca tgccgtgtcg tggaacaacg gcgtggaggg
    10681 caccaccgag gatcgcaagg tcgtcgcatt gcgctatcgg cagcagcgca acctgctggc
    10741 gacgttgctg ctgtcgcagg gcgtgccgat gctgcaggcc ggcgacgagt tcggccggac
    10801 gcagcgcggc aacaacaatg cctattgcca ggataacgag acgtcctggg tcgactggcg
    10861 cctggcgcgc agcaattcgg ctttgctcga gttcgtgcgg cacctgctga ggattcgtgc
    10921 cgacaacgcg gtgttccggc gccagggctt cctggccggc gtacaacgtg aggccggtcg
    10981 tttcaaggac gtcgtctggc tcagcagcga cggccaggag cttgccgagc cggactgggt
    11041 cgaccccggc cggcaggcct tcgccatgct gctggacagc accggcctgc cgccgaccca
    11101 gctgatgcct gacgtgggcg acagcttcct ggtgttgttc aacgccgcgc ccgaagcagt
    11161 cgagttcacc cttccggcgc cactcagcaa cgccgtctgg gaggtcgtgc tggataccag
    11221 ccaggaggcg ctcaccgtgc ccgccgaagg ctatcgccag ggccacggct acctgcttgc
    11281 ggaccgttca ctggcgctgc tggtggacca tggctgacag gggcgagctc ctggaccgcc
    11341 tgaggcggct caggggcatt gcaggcagct tcaccgacta ccgcggcgaa acgcatgagc
    11401 tgggcgagtc gtcgctgctc gggctgctgg cggcgctcgg acacacggtc gacaatccgg
    11461 cagcgctcgt gcgcgaagcc gaggccctcg agacgcgcga ctggatccgc gtgctcgggc
    11521 cggtggaggt gctgcgcggc tcacgcacag tgccgttcac agtgctggcg ccgctgttgc
    11581 cggcgatccg ctggcgggtg acgacggagt cgggcgacac gctcgacgga gagatcgtgc
    11641 cggcagcgct gcccgtgctc gaggaacgcg gcatcggggc gctctggtac gtgcggctcg
    11701 cgctcgaact gccgcagctg cccgccggct accaccgcct gtggctggaa aagcccgacg
    11761 gacagccgct cggcaacacc cgcctcatcg tggcgccgga gcgctgcttc gaaccgatcg
    11821 tcatccgcga cggcggacgg ccgtgggggc tggcggtgca gctgtacgcc ctgcgttccg
    11881 ggcgcaactg gggcatcggc gattttaccg acctggccgg attcgccgcg gcagcggggg
    11941 ggctcggggc cgacttgatc gggctgaacc cgctgcatgc cctttttccc gccgacccgg
    12001 cgctgtgcgc gccctacagt ccttccagcc gctatttcct caacgcgctg tacatcgatc
    12061 ccgaagccat acccgagttc cgggactccg tggaggcgca gcgactggtc ggcacgccgg
    12121 agttccaggc acggctcgag gcgctccggg ccgcgccctt cgtggactat cccggcgtga
    12181 cggcctgcaa gctggaagta ctgcggaccc tgcattccga gtttgccacc cgtgcctcga
    12241 ccgcacgccg gcacgacttc aatgaatttc tgaagaaaca cggccaggtc ctggaaagat
    12301 ttgccgtgtt tcatgcgctg cacgaacaat ttgtcgcccg cggcgagcgc ggcggctggc
    12361 ccgagtggcc ggccgggtac cagcggcccg gcgagcccgc cgtgcaggcc tttgccgagg
    12421 tcgagccggc cgccgtggat tttcactgct ggctgcagtg gaccgccgcc agccagctgg
    12481 cggccgcgga gcaatgcgcc cgcgcggcgg gcctgcggct cggcctctac cgggatctcg
    12541 ctgtcggccc caacggcggc ggcgccgaaa catggaccgc gcgcgagctc tacgctcggg
    12601 gggcaacggt cggcgctcca ccggacccgc tggcactgca gggacaggac tggggcattc
    12661 cgccgttcga tccggacgcc ctcagggagc gggcctatgc gcccttcatc cgcctgctgc
    12721 gcgccaatat ggggcaagac ggggcgctgc gcatcgatca cgtgatgatg ctgatgcggc
    12781 tgtggtgggt gccgcgcggc atgccctcgt cggccggcgg ctatgttcat taccgcctcg
    12841 acgagttcat gggcattgtc gccctggaaa gccagcgccg gcggtgcctg gtggtcggcg
    12901 aggacctggg cacggtgccg ccggaggtgc gcacggccat gagcgcgcac gggctgtaca
    12961 gttatcgcgt gctgctgttc gagcgcgagg ccgatggccg cttccgtgcg cccgcggact
    13021 acccgcgcga ggccctggtg acaccgagca cgcatgacct gcccacgctg gccagtttct
    13081 gggcgggcac ggacatcgag ctgcggcaac ggctggcgct gtatccggaa cccgggcttg
    13141 ccgaggaaca gcgtcgcgca aggcaggatg cgcgcgaagc gctgcttgcc gccctgcgtg
    13201 atgcggggct gcaggccgtg gcgacgaacc tgccgccgac atgcgaagtg cagcgatttc
    13261 tcgccatggc gccggccgcg gtgctgatgc tccagccgga agactggctc agcatggaga
    13321 ccccggttaa cgtgccgggg acgcacaccg agtacccgaa ctggggccgc aagttatcgg
    13381 ccgactggcc cgaattcatg cagcgcgcgg acctgcgcgc cttcgcggca gacatcagcc
    13441 gcctccgcca gtccggctga atggccgggt cgtaatttac gaactgacat caggtatatc
    13501 tcttaattga cggattttgc ggaaatatta gccggcacag aggccgtcga gcgagtcccg
    13561 gccaaggcgc ttgccgaggc gctcgcgagt ggcgcgctga tcgtgaccgc cacgcggcgc
    13621 ctggcgcgcc ggctgcaggc cgagcacgcg cgggccatgc gcgagccggg ctgggacacg
    13681 ccggccgtgg tgccgtggtc cgcctggctg caatcgacct acgctgactt gcgcgacttc
    13741 ggggcgctgg ctacgccgca gccctgcctg gacgactggc agtcggcagc cttgtgggag
    13801 gaggccttcc atgccgaccc ggtcgccggg acgctgctga tggcaggcgg cgccgtggac
    13861 gggtttcgcg acgcctggcg cctggtgcag gaatggcggc tcccgtggcc ggagctgcag
    13921 aagcgcgccg gtgaggattg cggcgtgttc ctgcgcctcg ccggccgtta ccgtggccgg
    13981 ttgcaagcgc tggactgcat cgaccgggcc ggcctgccgg ccgtccttgc cacggccttg
    14041 caggaccgga gcgggcccga cgttttcttt gccggcttcg acgcgctgca tcctgcgcag
    14101 gcgctgttga tccgctcgct cgggcggcgt gcgaagctgg ctgcgccgcc ccaccatgcg
    14161 gccgcgccga cgcgcgccgc gtacccggac agccggcggg agctggcggc ggcggcggcc
    14221 tgggcgcggc gacgcctcga gcaggacccc ggcgcgcgcc tgggaatcgt cgtgccggat
    14281 ctcgatgccc aggcgtccct gctggaagac ttgctggacg aggccctggt gcccgcgcgg
    14341 ctgctgccgg ggcggagcga tgtgccccgg ccgtggaacc tgtcgctcgg tcgaccgctg
    14401 gcggacgcgc cgatcgtggc ggcagccctg ctggcgctcg ggtcgacgag ccgggacggc
    14461 atcgagacgg ctgaggcgag ccgactgctg cgctcgccgt ttttcggcgg ggccgccgcg
    14521 gaggccggcc accgcgcgcg cctcgaggcc tggctgcgcg gccatgcgcc ggaccgcatt
    14581 ccgatcgcag cgctgccagg ctggctggcc gggcgggccg gcgccccgga atgcccgcag
    14641 ttcgcggacg gcattggcgc ctgtctcgag gaagtccggc gcgggccgcg ccggcagcgg
    14701 ccctcggact gggcagcggc catgacgcgc gcattggccc ggctcggctg gccgggcgat
    14761 acgcccctcg acagcgaagc ctggcagacg gtgcaggcct ggagcgaact gctggaggat
    14821 ttctcgcgac tcgatgtcgt tgccgggccg ctgaccttcg ccgacgcgct gacgaaggtg
    14881 cggcgcatgg gcgtagagcg gcgtttccag cccgagacgc ccgaagtgcc ggtgcaggtc
    14941 ctcggcctgc tggagaccgc cggactggaa ttcgacggcc tgtgggtcac ggggatgcat
    15001 gacggcgccc tgccggcccc gctgcggccc tgcgcgttgc tgccggcggc cctgcagcgg
    15061 gaactcggca tgccgcgtgc ctgcccggac accgagctgg cattggcccg acgcatggtg
    15121 acccgcttgt caggggcggc gcgcgaggtg cgattcagtc accctgcaac acgccaggac
    15181 gagccgctgc ggccgagccc gatcatcgct gcgctgccgg ccgcgacgga cagcgacgat
    15241 ttcacgcggg tgggcgcggc cgcgcgggcc tttgcgacgc ggcgcctgga gtcgcggccg
    15301 gacgacacgg ggccgcctgt caggggtgag gtcggcggcg gcaccggctt gctggcggcc
    15361 cagtcggcat gtcccttccg ggctttcgcg atgcatcgcc tcgccgcgca tgagctggcg
    15421 cggcctgaag ccggcatcga cggcctgacg cgcggcagtt tcatgcatga agcactgcgc
    15481 ttcctgtggg gcgaactgcg ggaccaggcc ggcctggcct cgctcgaccc gccggcaaga
    15541 atggcgcggg tcagggccgc cgtcgagcgc gcggcaagca ccgcgttggc cggattgccg
    15601 ccgggtctcg tgggcatcga actcgccgag gccgtccgca gaatcagcga gttgctcgag
    15661 gtagagctgt tgcggccccc cttcgaagtg ctgcagcgtg aggaacgcgt ggcgatcgaa
    15721 atcggaccgc tgcgcatcaa cgggcaggtc gatcggatcg accggacaac gcacggcgtg
    15781 gtgatcatcg actacaagac cggccgcgtg ccttcgccca aggaactgga gggcttgt
//
LOCUS       DAOMPG010000030        15757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1622_length_15757_cov_4.185964, whole genome shotgun sequence.
ACCESSION   DAOMPG010000030 DAOMPG010000000
VERSION     DAOMPG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_15757_cov_4.185964"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1214
                     /locus_tag="PLS34_02750"
     CDS             84..1214
                     /locus_tag="PLS34_02750"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRP86426.1"
                     /translation="MKKWISFPRVEGTASRQAHADLPPGTYERELGKEGFFGPATHMY
                     HRRPPTGWSDFEGPLRPRAFDTTRLAADADTPWGARLLLHNPAVKFRHWTLARSMPAL
                     ARNSDGDELLFLHAGTARLFCDYGHLELAEGDYLVLPRGTMWRLEVDAPVQVLLIEAT
                     NESYRLPDKGMLGPHAIFDEAMLDTPVIDDAFLAQQDEREWRVEVKRRDQVSVITYPW
                     NPLDAVGWKGDLAPVRINWRAIRPLMSHRYHLPPSVHTTFVAGRFVVCTFVPRPFESD
                     PDALKVPFFHNNDDFDEVLFYHAGEFFSRDNIHPGMITLHPCGFTHGPHPKALKNMLS
                     QPAAGTNEVAVMLDTRDALEVADDALGIEWPGYVDSWKGGGA"
     gene            1211..2197
                     /locus_tag="PLS34_02755"
     CDS             1211..2197
                     /locus_tag="PLS34_02755"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRP86427.1"
                     /translation="MKLGSLKSGGRDGTLIVVSADLSRAAPAVDIAPTLQAALDDWDR
                     SAGGLRRLAEDLAAGRAPGAFALRIEDLAAPLPRAYQFLDGSAYLAHVERVRRARGAE
                     MPPSFLHDPLMYQAVSDGFLPPVGTIPVASEEFGIDFESEVCVVTDDVPMGVSPADAA
                     GHVRLVCLVNDVSLRNLIPGELAKGFGFLQSKPRSALSPVMVTPDELGDAWRDGKLHL
                     PLLTWLDGERFGAPEAGVDMQFSFHELIAHAARTRPLAAGTIIGSGTVANQDESLGAS
                     CMAEKRMLEIIRDGQPSTPFLSFGARVRIEMLDRDGRSIFGAIEHVYEPYRR"
     gene            2172..2861
                     /gene="maiA"
                     /locus_tag="PLS34_02760"
     CDS             2172..2861
                     /gene="maiA"
                     /locus_tag="PLS34_02760"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRP86428.1"
                     /translation="MSTNPTAADPAPARLYGYWRSSAAWRVRIALNLKGLTWESIPVH
                     LVRDGGEQRAPAYLALNPQGLVPALEIDGQLLTQSLAIIEYLDETRPRPPLLPADSSG
                     RARARSLAQLVVADIHPLNNLRVLQWLERHGGLDDARREAWYRHWVVEGLGALEARLA
                     GEAATGRFCHGDAPGLADCCLVPQLYNARRYAVGLEPFPTLLRIEAACMALDAFQAAA
                     AESQADAVPAP"
     gene            2934..3410
                     /locus_tag="PLS34_02765"
     CDS             2934..3410
                     /locus_tag="PLS34_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86429.1"
                     /translation="MSAFSLNKIPDGPGVYALYEQDRPTLVGSAPNLRSLAEAHLVGS
                     RGPSDELRQVLPHPERLTRISWWQHPALADDGRRNAARWVAIEVLAPVSRPPFNLSGL
                     GEVALKDPEFVKAMNALFHGPPAGMFVPQSLDEIARSVYELKDKVAELERRLAEKG"
     gene            complement(3421..4050)
                     /locus_tag="PLS34_02770"
     CDS             complement(3421..4050)
                     /locus_tag="PLS34_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP86430.1"
                     /translation="MKLYDMRVAPNPRRVRIFLAEKGIEIPTVEINIRAGENLAPAFL
                     AVNPRGVLPTLQLEDGTVIDESTAICRYFEELRPDPPLLGRGALDKAMVECWTRRVEA
                     DAGQPVVDAFRNSYPPYADRAVPGRKGIRAIPELVERGRQRLADFYPLMDQRLGATEF
                     VAGAGFSFADITLLCIVDFARAIKLPTPDGLAHFERWHAQVSARPSAAA"
     gene            complement(4117..5700)
                     /locus_tag="PLS34_02775"
     CDS             complement(4117..5700)
                     /locus_tag="PLS34_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86431.1"
                     /translation="MNRCLAAALLCLAVSLPGQAADGVLRVANPGHPASLDPHRITGV
                     WENRVVGDMLMGLTTEGPGGEVDGGAASDWEVSEDGLRYEFRLREQRWSDGPPVTADD
                     FVYSFRRMMSGETTSPYAQFFWVIENGREVTAGDLPPSALGVAALDADRLEIRLTRRT
                     AYFPGLLMHFAAFPVPRHAVEAHGRSWTEPGRMPANGPFRLAARVPNDHVLLEKNPHF
                     HAADDVRLDGVRYYGLEDRDAALLRFRAGELDVLRDFPAGRTGWLREHMPETVRTAPY
                     LGLSFLAVNHEREALRDRRVRTALSLAVDRRIITERVLGSGEQPAWSLVPPGSSGYPQ
                     PAQAPWREEPMPARQARARALMEAAGYSRSRPLRLRLRYPVSENERRVAIALQSMWRG
                     ILVATELERAETAIHYAALQQGDFDLGLASWLAVYDDPQTFTLLLQTESGPNNLGRYS
                     DAQYDRLTDEAAREAEPASRAALLREAEALAMREQALIPLYHHAARNLVSTRVLGWQD
                     NLLDVHRSRYLGLASGGAE"
     gene            5770..6405
                     /gene="pdxH"
                     /locus_tag="PLS34_02780"
     CDS             5770..6405
                     /gene="pdxH"
                     /locus_tag="PLS34_02780"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRP86432.1"
                     /translation="MDISDLRNEYSGAPLRRADLDPDPGKQFAKWFEQARQVKQPEVN
                     ACTIATAGLDGRPNARTVLLKYFDADGFVFFTNLQSRKAREMAENPSVAMLFFWQTLE
                     RQVCIRGKVERVSATEAMKYFARRPRDSQLGAWVSEQSAIISTRTVLEMKMEEFKRKF
                     AAGEVPLPSFWGGFRVRPEEIEFWQGRASRLHDRLRYRRVDDTWVIERLAP"
     gene            complement(6383..7378)
                     /locus_tag="PLS34_02785"
     CDS             complement(6383..7378)
                     /locus_tag="PLS34_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HRP86433.1"
                     /translation="MLLSLARQRRILFFGGKGGVGKTTVASCVALAEAEAGRRVLVIS
                     TDPAHNLGHLWDRAVGPRAVRLAPNLDGVELDPERVVEEHLAEVGAALRRLMPEHLAG
                     EVDRHMALSREAPGMQEAALLERIAESVEQGLRDYDLLVFDTAPSGHTARLMALPEMM
                     AAWTEGLLARRERAGRFDQALANLASDQHLGDHVLGGGGESGTRDDRIRHILNRRRER
                     FARLRDTLADPEVTAFIIVLAAERLPVLETIELERKLRAAGITVGALVINKRAPPDPG
                     DFLAERRAQEERHLATLSEALPALPRLDLPLAPRDVVGATALTAFAAELRARDAR"
     gene            complement(7369..7641)
                     /locus_tag="PLS34_02790"
     CDS             complement(7369..7641)
                     /locus_tag="PLS34_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015401855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cory-CC-star protein"
                     /protein_id="HRP86434.1"
                     /translation="MGEDRRDGLLEQLSRGLHEYYVAPYRRGFARLKREEEDLFMLLV
                     FAETLGVPNPASWYTMELMPLLYEQFHDWHRRMGMEHSPLDHLRCC"
     gene            complement(7647..9320)
                     /locus_tag="PLS34_02795"
     CDS             complement(7647..9320)
                     /locus_tag="PLS34_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRP86435.1"
                     /translation="MNAVTMLLFGLAMLALGYLFYSKFIAEKIYRLDPDFRTPAHEFR
                     DGVDFVPTNKYILWGHHFTSVAGAAPIVGPAIAVIWGWGPAFAWVIFGTIFFAGVHDF
                     GALWASVRNKGKSIGTLTGSVIGARARNLFLVVIFLLLLMVNAAFAVVIANLLVSTPT
                     AVIPTWGAILVALVIGQMIYRYQMSLLWVSIIGVVALYALIFVGNATPIVLPESVMGL
                     GPRSQWILLLFAYAGIASILPVWVLLQPRDYINGLQLFLGLGLLYVAVLVSAPEIVAP
                     AFNDNVPAGTPSLVPLLFVTIACGAISGFHGLVSSGTTSKQLDKETDARFVGYFGSVG
                     EGMLALAAIICTTAGFATLADWEAMYSRFGGGGVVAFVKGGDAILMAGFGVPEQLGAT
                     MLAVMAILFAATTMDTGLRLQRYVIQEAAELGGIKVNVVLSTLIALGCCLALAFGAGA
                     DGAGGMIIWPLFGTTNQLLAGLTLSIISVILIRQGRPAWFTAVPLAFLLTMSVYALLV
                     QLGQFYQQKNWLLLGMDIVILLAALWVALEAAIAMSRGTKGAAAAQQRG"
     gene            complement(9360..10103)
                     /locus_tag="PLS34_02800"
     CDS             complement(9360..10103)
                     /locus_tag="PLS34_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86436.1"
                     /translation="MARSPSAPPASLVIGKRFCGPPESGNGGYTCGLLAAALPPPVEV
                     TLRRPPPLERKLAVLRSPDGVALTDGDATVAEARGAPFSLEVPHCPGLGAATEAAARY
                     HGLVEHAFPTCFVCGTGREHGDGLRIFAGEVAGDGLVASPWTPDQALADAQGAVRPEF
                     VWAALDCPGAWAWLAELERPLVLGRLAVVVDAPVLAGRPHIVGGWRLGHDGRKHYSGT
                     AIWAADGKVCARARATWIEIDPGRFGANG"
     gene            complement(10114..10869)
                     /locus_tag="PLS34_02805"
     CDS             complement(10114..10869)
                     /locus_tag="PLS34_02805"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRP86437.1"
                     /translation="MLAAKQTVLVLALSTTAVAACGRSERPTEPPQQVKPPTAQLDAP
                     ADQEPPPDLEALIDAGTAVGSFSFEAYAYDCEGLRIAVRPGDGELTLMLPDRAIVLPQ
                     VEAASGARYTDGRHGFWGKGVDRALLTLDGEDRQCELDRRETPWVDARARGAVFRGVG
                     QEPGWQLEIHPERIVMVYQYGERRVVVPNGGVITDADHPRRRWQATTEAHELAVVVED
                     RGCTDVMSGETYPASVAVVLDGRNYSGCGRDLD"
     gene            complement(10941..11618)
                     /locus_tag="PLS34_02810"
     CDS             complement(10941..11618)
                     /locus_tag="PLS34_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005302815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRP86438.1"
                     /translation="MRTELTPLEARVIGSLIEKEITTPDQYPLTLNALTAACNQKSNR
                     EPVLELSGGEVQTVLDGLMKKHLVTDRSGGVGGRVTRYRQRFCNTQFGNLHLSPPELA
                     IVCELLLRGPQTPGELRSRASRMAPLRDVSEVDAALAALAAREPPLVAQLPREPGRRE
                     SRWMQLFTGEAPPEPVVARDVPAPRATADAARLDELESRVTALQHEIEALQRKLDAFS
                     ARLDRHG"
     gene            11729..13639
                     /locus_tag="PLS34_02815"
     CDS             11729..13639
                     /locus_tag="PLS34_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP86439.1"
                     /translation="MLLRFDQVSLRFGDQLILSAADFGIEPGERVCLIGRNGAGKTSL
                     LKLISGTLSPDDGAIQARAGLRISRLEQTLPDELDLTVRESVMLGLAGVRGLVDDYQR
                     LAGRALDKLGLQQLEELHRRIDAVDGWHIERAVDTMLTEMNLPAGRRLGELSGGWRRR
                     AALARALVGKPDLLLLDEPTNHLDLSSIQWLEQRVAAWPGAVLFITHDRAFLQRLATR
                     IIEIDRGKLVSWPGDYREYLRRKAEAIEEEERHNAEFDRKLAEEEAWIRQGIKARRTR
                     NEGRVRALMELRDIRAQRVSRPGSARIRIGEADSSGRKVIQAKNVSYGYGGEPLISGL
                     DLKIMRGDRIGLVGNNGVGKSTLLRLLLGELEPQAGTIKHGTNLEIAYFDQLRGQLDA
                     DKSVAHNVGDGRDHVTINGKPQHVVGYLRGFLFSAKRALTPVGSLSGGERNRVILAKL
                     FTRPSNLLVLDEPTNDLDVETLEVLEERLGEYSGTLLAVSHDREFLDNVVDRILVFEA
                     GGRIVEYAGGYSDWARRGRELAQLDAEAQAPASSPVAPPEERRRVRKPAKLGYLEQRE
                     LDLLPERIAAVEAEIAALEAEIAAPDFYTRSWEQTQPVLDRLAERNTEQDRLTERWLA
                     LEDRQAALAAGD"
     gene            complement(13649..13855)
                     /locus_tag="PLS34_02820"
     CDS             complement(13649..13855)
                     /locus_tag="PLS34_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HRP86440.1"
                     /translation="MSDDRFEQLELKLAYLERAAQELGDVVYRQQQALDALAARLERL
                     AEQVRANAEAARPYTDAEEAPPHY"
     gene            complement(14269..15216)
                     /locus_tag="PLS34_02825"
     CDS             complement(14269..15216)
                     /locus_tag="PLS34_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86441.1"
                     /translation="MRRIAWVVLALLAASVGWAAWWLADHARPRHEAFVDRRGGEISA
                     ELEAPVTEPGGFISQGVHISSDSGLRVDARVLRPAMQDGPLPVLVLLGGHRTGRDAVA
                     LVGHPGETVMVALDYPYHGTLEVRSTASFFRAIRDIQQALLDTPPAASTVLDWLATQG
                     WADTGRAELVGVSFGVPFVAVAGALDPRFRRVWFIHGGAGNRGWIEHNLRDRVPQRWL
                     RPLLASLVHLLVYGNSFDTEEWVARIAPREVVVIGARDDERLPPHKVVNLYEAAGQPK
                     ELQWTEGGHVDPARPDIVRALLAIVRERLDESARPPAAP"
     gene            complement(15263..>15757)
                     /locus_tag="PLS34_02830"
     CDS             complement(15263..>15757)
                     /locus_tag="PLS34_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP86442.1"
                     /translation="GVEVDVVVSDVRMPGVDGLELCRRLREARGIPVVLLTTFDEEGL
                     VVAAAAAGAHGFLLKDASPADLAAAIHKAARGEDWLQPVATEPVRERFAYHDSSPPSG
                     AFSERELAILRLMAGGYSNREIAATLFLAEGTVKNYVSEILAKLETRDRTRAVLKAIT
                     LHII"
BASE COUNT         2494 a         5470 c         5519 g         2274 t
ORIGIN      
        1 cccattccgc gctcgccggc tggaagccat gttgctcgag gcgacgaaga gccggactgt
       61 atcccgggca gaggaaactg ggcatgaaga aatggatctc tttcccgcgg gtggagggca
      121 cggcctcgcg ccaggcacat gccgacctgc cgccgggcac ctacgagcgc gaactgggca
      181 aggaaggctt cttcggcccg gcgacgcaca tgtaccatcg gcggcctccc accggctggt
      241 ccgacttcga gggaccgctc aggccgcgcg ccttcgacac caccaggctt gccgccgacg
      301 cggacacgcc ctggggcgcc cgcctgctgc tgcacaaccc tgcggtgaag ttccggcact
      361 ggacgctggc caggagcatg cccgcactgg cccgcaactc cgacggcgac gaactgctgt
      421 tcctgcatgc ggggacggcg cgactgttct gtgactacgg gcacctggag ctcgccgagg
      481 gcgactacct ggtgttgccg cggggcacca tgtggcgcct cgaggtcgat gcgccggtgc
      541 aggtactgct catcgaggcg accaacgaga gctatcggct gccggacaag gggatgctcg
      601 ggccgcacgc catcttcgac gaggcgatgc tcgacacacc ggtcatcgac gacgccttcc
      661 tggcgcagca ggacgagcgc gagtggcgcg tggaagtgaa acgtcgcgac caggtgtccg
      721 tgatcaccta tccctggaac ccgctggacg cggtcggctg gaaaggcgac ctcgcgccgg
      781 tgcgcatcaa ctggcgcgcc atccgcccgc tgatgtcgca ccgctatcat ctgccgccat
      841 cggtgcacac tactttcgtg gccgggcgct tcgtggtctg caccttcgtg ccgcggccct
      901 tcgagagcga tccggacgcg ctcaaggtgc ccttctttca caacaacgac gatttcgacg
      961 aggtgctgtt ctatcatgcc ggcgagtttt tctcgcgcga caacattcac cccggcatga
     1021 tcacgctgca cccctgcggc ttcacccacg ggccgcaccc caaggcgctg aagaacatgc
     1081 tgagccagcc cgcagcgggc accaacgagg ttgccgtgat gctggacacc cgcgatgccc
     1141 tggaggttgc ggacgacgcc ctcgggatcg agtggccggg ctacgtcgac agctggaagg
     1201 ggggcggcgc gtgaagctcg gcagcctgaa gagcggggga cgggacggca cgctgatcgt
     1261 ggtcagcgca gacctgtcgc gggcggcgcc ggcggtcgac atcgcaccca cactgcaggc
     1321 ggcgctggac gactgggacc gcagcgccgg tggcctgcga cggcttgcgg aggacctggc
     1381 ggcgggccgc gcgccgggcg cgttcgcgct ccggatcgag gatctcgccg cgccgctgcc
     1441 gcgggcctac cagttcctgg acggcagcgc ctacctggcc catgtcgagc gggtgcgtcg
     1501 cgcacgcggc gccgagatgc cgccgagttt cctgcacgac ccgctgatgt accaggcggt
     1561 gtcggacggc ttcctgccgc cggtcggcac gattcccgtg gccagcgagg aattcggcat
     1621 cgacttcgag tcggaggtct gcgtggtcac cgacgacgtg cccatgggcg tcagcccggc
     1681 ggatgcggcg ggccatgtcc ggctggtctg cctcgtcaac gacgtctcgc tgcgcaacct
     1741 gatccccggc gagctcgcca agggcttcgg cttcctgcag agcaagccgc gctcggcgct
     1801 gtcgccggtg atggttacgc cggacgagct cggcgacgcg tggcgcgacg gcaagttgca
     1861 cctgccgctg ctcacctggc tggacggcga gcggttcggc gcgccggagg ccggcgtcga
     1921 catgcagttc tcctttcacg agctgattgc gcacgcggcg cggacccggc cgctggcggc
     1981 cggcaccatc atcggttccg gcaccgtggc caaccaggac gagtcactcg gcgcttcctg
     2041 catggcggag aagcgcatgc tggaaatcat tcgcgacggc cagccgagca cgccgttcct
     2101 gtccttcggg gcccgggtcc gcatcgagat gctcgaccgc gacggccgca gcatcttcgg
     2161 cgccatcgag catgtctacg aaccctaccg ccgctgatcc ggcgccggcc aggctgtacg
     2221 gctactggcg cagcagcgcg gcctggcgcg tgcgcatcgc cctgaatctc aagggactca
     2281 cctgggaatc gatcccagta cacctggtgc gcgacggggg cgagcagcgc gcaccggcct
     2341 acctggcgct gaatccccag ggcctcgtgc cggcgctgga gatcgacggc cagctgctga
     2401 cccagtcact ggcgatcatc gagtacctcg acgagacgcg cccccgtccg cccctcctgc
     2461 cggccgactc gtcgggccgc gcaagggcca ggagcctggc gcagctggta gtggccgaca
     2521 tccacccgct caacaacctg cgggtgctgc agtggctcga gcgccacggc gggctcgacg
     2581 acgcgcggcg cgaggcatgg tatcgacact gggtggtcga gggactgggc gcgctcgagg
     2641 cgcgccttgc gggcgaggcc gcgaccgggc gtttctgtca cggcgacgcg cccgggctgg
     2701 cggactgctg cctggtgccg cagctctaca atgcacgacg ctacgcagtc gggctggagc
     2761 cgttcccgac gctgctgcgc atcgaggccg cctgcatggc gctggacgca ttccaggcgg
     2821 cggccgccga atcccaggcc gacgccgtcc ctgcgccctg actgcggaaa accgggatgc
     2881 ccggcgcggt gaaagggcta gaatagaggg cgacgcagcc agggacgatc gccatgagcg
     2941 ccttcagcct taacaagatt cccgacggcc cgggcgttta cgccctctac gagcaggacc
     3001 ggcccacgct ggtcggttcc gcgccgaacc tgcgctcgct ggcagaggcc cacctggtcg
     3061 gcagccgcgg gcccagcgac gagctgcgcc aggtgcttcc ccatcccgag cggctcaccc
     3121 ggatcagctg gtggcagcac ccggcgctgg cggacgacgg tcgccgcaat gccgcccgct
     3181 gggtggccat cgaggtgctg gcgccggtca gccggccgcc cttcaacctg tccgggctgg
     3241 gcgaggtcgc gctcaaggat ccggaattcg tgaaggccat gaacgcgctg ttccatggcc
     3301 cgccggccgg catgttcgtg ccgcagtcgc tcgacgaaat cgcgcgcagc gtctacgagc
     3361 tcaaggacaa ggtcgcggag ctggagcgcc gcctggccga aaaaggctga gccgcgcggt
     3421 tcaggccgcg gcgctgggcc gggccgatac ctgggcgtgc cagcgctcga agtgcgccag
     3481 gccgtcgggg gtcggcaact tgatggcgcg tgcgaaatcg acgatgcaca gcaaggtgat
     3541 gtcggcgaag gaaaagcccg cgccggccac gaattccgtc gcgccgagcc gctggtccat
     3601 cagcgggtag aaatcagcca gccgctgccg gccacgctcg accagctccg gaatcgcccg
     3661 gatgcccttg cgcccgggaa cggcgcgatc cgcgtagggc ggatagctgt tgcgaaaggc
     3721 gtctaccacc ggctgcccgg cgtcggcctc cacgcggcgc gtccagcatt ccaccatcgc
     3781 cttgtcgagc gcgccccggc ccagcagcgg cgggtcgggg cgcagttcct cgaaataccg
     3841 gcagatggcg gtgctctcgt cgatcaccgt gccgtcctcc agctgcaggg tcggcagcac
     3901 gccgcgcgga ttgacggcca ggaacgccgg cgcgagattc tcgccggcgc ggatgttgat
     3961 ctccaccgtg ggaatctcga tgcccttttc ggcgaggaag atgcgtacgc gacgcggatt
     4021 cggcgcgacg cgcatgtcgt acagtttcac ggccgaagcc ctcctggagt ctgtcgcaaa
     4081 gcataacccg gccgcgcccg cggcggacct gccgggtcac tcggcgccgc cggatgccag
     4141 gccgaggtaa cggctgcggt ggacgtccag caggttgtcc tgccagccca gcacgcgcgt
     4201 gctcaccagg ttgcgcgcgg cgtggtgata aagggggatc agcgcctgct cgcgcatggc
     4261 gagcgcctcg gcttcacgca gcagggcggc gcgggatgcc ggctccgcct cgcgcgcggc
     4321 ctcgtcggtg agccggtcgt attgcgcgtc ggagtaacga ccgaggttgt tcgggccgct
     4381 ctcggtctgc agcagcagcg tgaaagtctg gggatcgtcg tagaccgcga gccagctggc
     4441 gaggccgagg tcgaagtcgc cctgctgcag cgcggcatag tgaatcgccg tctctgcgcg
     4501 ctcgagttcg gtggccacca ggattccccg ccacatggat tgcagggcga tcgccacgcg
     4561 ccgctcgttc tcggaaaccg ggtaacgcag ccgcaggcgc agcgggcggc tgcgactgta
     4621 tccggccgct tccatcagcg cgcgcgcccg cgcctggcgc gccggcatcg gctcctcccg
     4681 ccagggtgcc tgcgccggtt gcgggtagcc ggagctcccc ggtggtacca ggctccaggc
     4741 cggctgctcg ccgctgccca gcacccgctc ggtgatgatg cgccggtcca cggccagcga
     4801 tagcgctgtg cggactcgcc ggtctcgcag cgcctcgcgc tcgtgattca cggccaggaa
     4861 ggaaaggccg aggtacgggg ccgtgcgtac cgtctcgggc atgtgctccc gcagccaccc
     4921 ggtgcgtccg gccggaaagt cgcgcaatac gtcgagttca ccggcgcgaa agcgcagcag
     4981 ggccgcatcg cggtcctcga ggccgtaata ccggacgccg tcgagccgca cgtcgtcggc
     5041 ggcgtggaaa tgcgggttct tctccagcag cacgtgatcg ttgggcaccc tggccgccag
     5101 gcggaacggg ccgttggccg gcatccggcc gggctcggtc caggagcggc catgcgcctc
     5161 gacggcgtgg cgcggcaccg ggaaggccgc gaagtgcatc agcaggccgg ggaagtaggc
     5221 ggtcctgcgg gtcagccgga tttccagccg atccgcgtcc agcgccgcca ccccgagggc
     5281 cgacggcggc agatcgccgg cagtcacctc gcggccgttc tcgatgaccc agaagaactg
     5341 agcgtagggc gaggtggtct cgcccgacat catgcgacgg aaggaataga cgaaatcgtc
     5401 ggcagtgacc ggcgggccgt ccgaccagcg ctgctcccgc agccggaatt cgtagcggag
     5461 cccgtcctcg gacacctccc agtcgcttgc cgcgccgccg tccacctcgc cgcccgggcc
     5521 ctcggtggtg agccccatca acatgtcgcc gacgacgcga ttctcccaga ccccggtgat
     5581 gcgatgcgga tccagcgagg ccgggtgacc cggattggcc acgcgcagga ccccgtccgc
     5641 ggcctggccg ggcagggaca cggcaaggca cagcagcgcg gcggccaggc agcggttcat
     5701 gcggatctcc cgagcgcgat ttgcgaacta gaataacaac tgatgccgcc gaccacaggg
     5761 agtgctggaa tggacatcag tgacttgaga aacgaataca gcggtgcgcc gctgcgccgc
     5821 gcggacctcg acccggaccc gggcaagcag ttcgcgaagt ggtttgagca ggcccgccaa
     5881 gtgaagcagc cggaggtgaa cgcctgcacg atcgccactg ccgggctgga cggccggccg
     5941 aatgcgcgca ccgtgctgct gaagtatttc gacgcggacg ggttcgtgtt cttcaccaac
     6001 ctgcagagcc gcaaggcccg cgagatggcg gagaatccct cggtcgccat gctgttcttc
     6061 tggcagacgc tggagcggca ggtctgcatc cggggcaagg tggagcgggt cagcgccacc
     6121 gaggcgatga agtatttcgc gcgccgcccg cgcgacagcc agctcggcgc ctgggtgtcg
     6181 gagcagagcg caatcatcag cacccggacg gtgctggaga tgaagatgga agagttcaag
     6241 cgcaaattcg cggccggcga agtgccgctg ccgtctttct ggggcggttt ccgtgtgcgg
     6301 ccggaggaaa tcgagttctg gcagggccgc gcctcgcgcc tgcacgatcg cctgcgttac
     6361 cggcgggtcg acgacacctg ggtcatcgag cgtctcgcgc cctgagctcg gccgcaaagg
     6421 cagtcagcgc cgtggcgccg accacgtcgc gcggagccag cggcaaatcg aggcgcggca
     6481 gtgcgggcag cgcctcgctc agggtagcga ggtgccgttc ttcctgggcg cgccgctcgg
     6541 ccaggaaatc gccggggtcc ggcggcgcgc gcttgttgat caccagcgcc ccgaccgtga
     6601 tgccggcggc gcgcagcttg cgctcgagct cgatggtctc gagcaccggc aggcgttctg
     6661 ccgccagcac gatgatgaag gccgtgactt cgggatcggc gagcgtgtcc ctgagccggg
     6721 cgaagcgctc gcggcgccgg ttcaggatat ggcgtatgcg atcgtcgcgc gtgccgcttt
     6781 ctccgccgcc acccagcacg tggtcgccga ggtgctggtc ggacgcaagg ttcgccagcg
     6841 cctggtcgaa gcgcccggcg cgctcgcgcc gcgccagcag gccttcggtc caggcagcca
     6901 tcatttccgg cagcgccatc aggcgcgccg tgtggcccga cggcgcggtg tcgaacacca
     6961 gcaggtcgta gtccctgagg ccctgttcga ccgactcggc gatgcgttcg agcagcgccg
     7021 cctcctgcat gccgggcgct tcgcgcgaca gcgccatatg gcggtcgacc tcgccggcga
     7081 ggtgctcggg catgagccgg cgcaacgccg cgccgacctc ggcgaggtgt tcctcgacca
     7141 cgcgctcagg atccagttcc acaccgtcga ggttgggcgc cagccgcacc gcccgcggcc
     7201 cgacggcgcg gtcccacagg tgccccaggt tgtgtgccgg gtcggtggaa atgacgagta
     7261 cccggcggcc ggcctcggcc tccgccagcg ccacgcagga ggcgaccgtg gtcttgccga
     7321 ccccgccctt gccgccgaag aacaggatgc gccgttggcg ggcgaggctc agcagcaccg
     7381 caggtgatcc agcggcgagt gctccatgcc catgcggcga tgccagtcgt ggaactgctc
     7441 gtagagcagc ggcatcaact ccatcgtgta ccacgaggcg gggttgggca cgccgagcgt
     7501 ctcggcgaac accagcagca tgaacaggtc ttcctcttcc cgcttgaggc gtgcgaagcc
     7561 ccgccggtac ggcgccacgt agtactcgtg cagcccccgc gagagctgct cgagcaggcc
     7621 gtcccggcgg tcctcgccca ccggcgctag ccgcgctgct gcgccgccgc cgcgcccttg
     7681 gtgccgcggc tcatcgcgat cgccgcctcg agcgcgaccc acagcgccgc cagcaggatg
     7741 acgatgtcca tgccgagcag cagccagttc ttctgctggt agaactgccc gagctgcacc
     7801 agcagtgcat acacggacat ggtcagcagg aaggccagcg ggacggccgt gaaccaggcc
     7861 ggccggccct ggcggatcag gatcaccgag atgatcgaca gcgtgaggcc cgccagcagc
     7921 tggttggtgg tgccgaacag cggccagatg atcatgccgc cggcgccgtc cgcgccggca
     7981 ccgaaggcga gtgccaggca gcagccgagc gcgatcagcg tgctgagcac gacattgacc
     8041 ttgatgccgc cgagctcggc cgcttcctgg atgacgtagc gctgcagacg caggccggtg
     8101 tccatggtgg tggcggcgaa caggatcgcc atcaccgcga gcatggtggc gccgagctgc
     8161 tccggcacgc cgaaaccagc catcaggatg gcgtcgccgc ccttcacgaa ggccacgacg
     8221 ccgccgccgc cgaagcgcga gtacatggcc tcccagtccg ccagcgtggc aaagcccgcc
     8281 gtggtgcaga tgatcgccgc cagcgccagc atgccctcgc cgacggagcc gaagtaaccg
     8341 acgaagcgtg cgtccgtttc cttgtcgagc tgcttcgatg tggtgcccga ggacaccagg
     8401 ccgtggaagc cgctgatggc gccgcaggcg atcgtcacga acagcagcgg caccaggctg
     8461 ggcgtgccgg ccggtacatt gtcattgaag gccggcgcga cgatctccgg cgccgagacc
     8521 agcacggcca cgtacagcag cccgaggccg aggaacagct gcaggccgtt gatgtagtcg
     8581 cgtggctgca gcagcaccca gacgggcagg atcgaggcga tgcccgcata cgcgaacagc
     8641 agcaggatcc actgcgagcg cggcccgagg cccatgacgc tctccggcag cacgatggga
     8701 gtggcgttgc cgacgaagat cagcgcgtac agcgccacca cgccgatgat cgagacccac
     8761 agcaggctca tctggtagcg atagatcatt tgcccgatga ccagcgccac caggatcgcg
     8821 ccccaggtcg ggatcaccgc ggtgggcgtg gacaccagca ggttggcgat caccaccgcg
     8881 aaggccgcgt tgaccatcaa cagcagcagg aagatcacca ccagaaacag gttgcgcgcc
     8941 cgcgcgccga tgacgctgcc ggtgagcgtg ccgatcgact tgcctttgtt gcggacactg
     9001 gcccacagcg cgccgaagtc gtgcacgccg gcaaagaaaa tggtgccgaa gatcacccag
     9061 gcaaaggccg gcccccagcc ccagatgacg gcgatggccg gtcccacgat cggtgcagcg
     9121 cccgcgaccg aggtgaagtg atggccccag aggatgtact tgttggtggg gacgaaatcc
     9181 acgccgtcac ggaactcgtg cgccggcgta cggaagtccg ggtccagccg atagatcttc
     9241 tcggcgatga acttggagta gaacaggtag ccgagcgcca gcatggcgag cccgaacaac
     9301 aacatcgtca cagcattcat agccccgtcc ccctgatgtg aatcgtcgtc accgtagcgt
     9361 caaccgttcg ccccgaatcg cccgggatcg atttcgatcc aggtcgcccg ggcgcgtgcg
     9421 cacaccttgc cgtcggcggc ccagatcgcg gtcccggagt aatgcttgcg gccgtcatgc
     9481 ccgaggcgcc agccgccgac gatgtgcggc cgcccggcca gcaccggcgc atcgacgacc
     9541 accgccagcc ggcccagcac gagcggtcgc tcgagctctg ccagccaggc ccaggcgccg
     9601 ggacaatcca gcgccgccca gacgaattcc gggcgcaccg cgccctgcgc gtcggcgagt
     9661 gcctgatccg gggtccatgg cgacgcgacc aggccgtcac ccgcgacttc gccggcaaag
     9721 atgcgcaagc cgtcgccatg ctcacggccg gtgccgcaga cgaagcatgt cggaaaggcg
     9781 tgctcgacga gcccgtggta gcgcgccgcc gcctccgtgg cggcgcccag gcccggacag
     9841 tgcggcacct ccagcgaaaa tggcgcgcca cgcgcctcgg cgaccgtggc gtcgccgtcc
     9901 gtcagcgcga ctccgtccgg gctgcgcagc accgccagct tgcgttccag cggtggcgga
     9961 cgccgcagcg tcacctcgac cggtgggggc aacgcggcgg cgagcaggcc gcaggtatag
    10021 ccgccgttgc ccgactcggg cggcccgcag aagcgcttcc cgatgacgag tgacgcgggc
    10081 ggtgcagagg gtgaccgtgc cacgcgcgtg ggcctagtcc aggtccctgc cgcagccgga
    10141 ataatttcgg ccgtcgagca ccaccgcgac gctcgcgggg taggtttcac cgctcatcac
    10201 gtcggtgcag ccgcgatcct ccaccaccac ggcgagttcg tgcgcttcgg tggtggcctg
    10261 ccagcgccgc cggggatggt cggcatccgt gatgacgccg ccgttgggca cgaccacgcg
    10321 gcgctccccg tactggtaga ccatgacgat gcgctcggga tggatctcca gctgccagcc
    10381 gggctcctgt ccgacgccac gaaacaccgc gccgcgggcg cgcgcatcga cccagggagt
    10441 ctcccgacgg tcgagttcgc actgccggtc ctcgccatcg agcgtcagca gcgcgcggtc
    10501 gacgcccttg ccccagaagc cgtgccggcc gtcggtgtag cgggcgccgg acgcagcctc
    10561 cacctgcggc agcacgatgg cacggtccgg cagcatcagc gtcagttcgc cgtcgccggg
    10621 ccgcaccgcg atacgcagtc cctcacaatc gtaggcgtaa gcctcgaagc tgaacgagcc
    10681 gaccgccgtg ccggcgtcga tcagcgcctc gagatccggc ggcggctcct ggtcggcggg
    10741 ggcgtcgagt tgcgcggtcg gcggcttgac ctgctgcggc ggctcggtcg gccgctcact
    10801 gcggccgcag gccgccactg ccgtcgtcga cagcgcaagc accaggacag tctgctttgc
    10861 tgcgagcata tcggccgttc ccccggcggc gaatcccgca atgctagcaa acctcccgga
    10921 ggggtcgcga atgcgtattt tcacccatgc cggtcgaggc gcgcggagaa tgcgtccagc
    10981 ttgcgctgca gcgcttcgat ttcatgttgc agcgcagtta cgcgactttc gagctcatcg
    11041 aggcgcgcgg catccgccgt ggcgcgcggg gcggggacgt cccgagccac aaccggttcc
    11101 ggcggcgcct cgccggtgaa cagctgcatc cagcgcgatt cgcgccggcc gggctcccgc
    11161 ggcaactggg ccaccagcgg cggttcgcgt gccgccagcg ccgccagcgc tgcatccacc
    11221 tcgctcacgt cgcgcaacgg cgccatgcga ctggcgcggc tgcgcagctc cccgggtgtc
    11281 tgggggccgc gcagcaggag ctcacagacg atcgcgagct ccggcggcga gagatgcagg
    11341 ttgccgaact gggtgttgca gaaacgctgc cggtaacgcg tgacccggcc gccgacgccg
    11401 ccgctgcgat cggtcaccag gtgcttcttc atcagcccgt cgagtaccgt ctgcacttcg
    11461 ccgccgctca gttcgagcac cggctcgcgg ttgctcttct ggttgcacgc ggcggtgagc
    11521 gcgttgagcg tcagcgggta ctggtccggc gtggtgatct ccttctcgat gagactgccg
    11581 atgacgcggg cttcgagtgg cgtgagttcg gtgcgcaaga gggctctccg caaggcaggc
    11641 cggtcggggc cgacagacta agggataacg acacgcggcg gtagcgggcg cgctagaatc
    11701 gcgctcccgt ccagtgtgcc gtgtgcccat gctgctccgc ttcgaccagg tttcgctgcg
    11761 cttcggcgac cagctgatcc tctcggccgc cgatttcggc atcgagccgg gcgagcgcgt
    11821 ctgcctgatc gggcgtaacg gcgcgggcaa gacgtcgctg ctgaaactga tatccggaac
    11881 cctctcgccc gacgacggcg cgatccaggc gcgcgccggg ctgcgcatca gccgcctgga
    11941 gcagaccttg ccggacgagc tcgacctcac ggtgcgcgag tccgtcatgc tggggctggc
    12001 cggcgtgcgc gggctggtgg acgactacca gcgactggcc ggccgggcgc tcgacaagct
    12061 cggcctgcag cagctggaag aactgcatcg gcgcatcgac gcggtggacg gctggcatat
    12121 cgagcgggcc gtggacacga tgctgaccga gatgaacctg cccgccgggc ggcggctcgg
    12181 cgaactttcg ggcggctggc gacgccgcgc cgcgctggcg cgcgcgctgg tcggcaagcc
    12241 ggacctgctg ctgctcgacg agccgaccaa ccatctcgat ctttcgtcca tccagtggct
    12301 ggagcagcgc gtggccgcct ggccgggcgc cgtgctgttc atcacgcatg accgtgcgtt
    12361 cctgcaacgg ctggccaccc gcatcatcga gatcgaccgc ggcaagctgg tgagctggcc
    12421 gggcgactac cgcgagtacc tgcggcgcaa ggcggaggcg atcgaagagg aggagcggca
    12481 caacgccgaa ttcgaccgca agctggccga ggaggaagcc tggatcaggc agggcatcaa
    12541 ggcacggcgg acgcgcaacg agggccgcgt ccgggcattg atggagctgc gcgacattcg
    12601 cgcgcagcgc gtcagccgcc cgggcagcgc gcgcatccgc atcggcgagg cggacagctc
    12661 cgggcgcaag gtcatccagg cgaagaacgt cagctacggc tacgggggtg agccgctgat
    12721 ctcagggctg gacctgaaga tcatgcgcgg cgaccgcatc ggcctggtgg gcaacaacgg
    12781 cgtcggcaag agcacgctgc tcaggctgct gctcggtgag ctcgagccgc aggccggcac
    12841 gatcaagcac ggcaccaacc tggagatcgc atacttcgac cagctgcgcg ggcagctcga
    12901 tgccgacaag agcgtggcgc acaacgtggg tgacgggcgt gatcacgtca ccatcaacgg
    12961 caagccgcag cacgtggtgg gttatctgcg cggcttcctg ttcagcgcga agcgcgcgct
    13021 cacgccggtc ggctcgttgt cgggcggcga gcgcaaccgc gtgatcctcg ccaagctgtt
    13081 cacgcggccg agcaacctgc tggtgctcga tgagcccacc aacgacctgg atgtcgagac
    13141 cctcgaggtg ctcgaggagc ggctcggcga gtactccggc acgctgctgg cagtcagtca
    13201 cgaccgcgag ttcctcgaca acgtggtgga ccggatcctg gtgttcgagg ccggcgggag
    13261 gatcgtcgag tacgccggcg gctacagcga ctgggcacgg cgcggccggg agctcgcgca
    13321 actcgatgcc gaggcccagg cgccggcctc gagtcccgtg gcgccgccgg aggaacggcg
    13381 cagggtccgc aagccggcca agctgggcta cctggagcag cgcgagctcg acttgctgcc
    13441 ggagcggatc gcggcggtgg aggcggagat cgccgcactg gaggccgaga ttgcggcgcc
    13501 ggacttctat acccggtcgt gggaacagac gcagccggtg ctcgaccggc tggccgaacg
    13561 gaatacagag caggaccggc tgacggaacg ctggctggcg ctggaggatc ggcaggcggc
    13621 gctcgcggct ggagactgag ggcgcgcctc agtaatgagg cggcgcttcc tccgcgtcgg
    13681 tgtagggccg ggccgcctca gcattggcac gcacctgctc cgccagtcgt tccaggcggg
    13741 ccgcgagggc atccagcgcc tgctgctgac ggtagacgac gtcgcccagt tcctgcgcgg
    13801 cgcgctccag gtaggcgagc ttcagctcca gctgttcgaa tcggtcgtca ctcaaggttg
    13861 cttcgctccg gcccgaaaag aggggacatt ccccaattcc attcggaatt ggggaatgtc
    13921 cccattgtcc accaaaaaga aaggggccgg caggccgacc cctttcgctc gcggtgcctg
    13981 ggattcacgc tggccgccgg gctcccctgt cgcggtgtgt ctccgcggcg cgccagaggc
    14041 cgcgttgcgg ctttgctcgg cgccatgccc gggtgcatgg tcctgcaccg gtgacgcgaa
    14101 cggaccggcg ccgggcgccg caggtctcag gcatgcgcgg gactcgacgt ggagtcctgc
    14161 aaccggtatc ccaccgggcc gcacgctgcc ttgtaatccg tccgcgtgca gcctgaacat
    14221 aggacagcca ccgccgggtg tcgctacggg ctttccacta cgtcggactc aaggcgcagc
    14281 gggcggcctg gcactttcgt cgaggcgttc gcggacgatg gccagcaggg cccggacgat
    14341 atccgggcgc gccgggtcga cgtgcccgcc ctcggtccac tgcagttctt tcggttgccc
    14401 ggccgcctcg taaaggttca ccaccttgtg cggcggcagc cgctcatcgt cccgggcgcc
    14461 gatgaccacc acttcgcgcg gggcgatcct cgccacccac tcctcggtgt cgaaggaatt
    14521 gccgtacacc agcaggtgca ccagcgacgc cagcagcggc cgcagccaac gttgcggcac
    14581 gcggtcacgc aggttgtgct cgatccagcc acggttgccc gcgccgccgt ggatgaacca
    14641 gacgcgccgg aagcgggggt cgagcgcgcc ggccaccgcg acgaagggca cgccgaaact
    14701 gacgcccacc agctcggcgc gaccggtgtc cgcccagccc tgcgtggcca gccagtcgag
    14761 caccgtcgat gccgccggcg gcgtgtccag caacgcctgc tggatgtcgc ggatggcgcg
    14821 gaaaaaactt gcggtggagc gcacctcgag cgtgccgtgg tacggatagt ccagcgccac
    14881 catcaccgtt tcgccgggat gaccgaccag cgcgaccgca tcgcggccgg tgcggtgccc
    14941 gccgagcagt acgagcaccg gcagcggtcc gtcctgcatt gccggccgca gtacgcgggc
    15001 atccacccgc agcccggaat ccgagctgat gtgcacgccc tggctgatga atccgccggg
    15061 ctccgtcacc ggggcctcga gttcggcgct gatctcgccg ccgcgacgat cgacgaacgc
    15121 ctcgtggcgg gggcgggcat ggtcggcaag ccaccaggcc gcccagccca cggacgccgc
    15181 gaggagcgcc aggacgaccc atgcaatgcg tctcatgggg cgcgattatc gcatgccggc
    15241 cggccccgcc cggccgctgg catcagatga tgtgcagcgt gatggccttc agcactgcgc
    15301 gggtccggtc gcgcgtctcc agcttggcga ggatctcgga gacgtagttc ttcacggtgc
    15361 cctcggccag gaacagcgtt gcggcgatct cgcgattgga atacccgccg gccatcagcc
    15421 gcaggatggc gagctcgcgc tcggagaagg cgccgctggg tggcgagctg tcgtgatagg
    15481 cgaagcgctc gcgcaccggt tccgtggcga ccggctgcag ccagtcctcg ccgcgcgccg
    15541 ccttgtggat cgctgcggcc aggtccgcgg gcgaggcatc cttcagcagg aagccgtgcg
    15601 ccccggcggc agcggccgcc accaccaggc cctcttcatc gaaagtggtg agcagcacca
    15661 cggggatgcc ccgcgcttcg cggagccgcc ggcagagctc gagcccgtcc acgccgggca
    15721 tgcggacgtc ggacacgacg acgtcgacct cgacgcc
//
LOCUS       DAOMPG010000031        15624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1645_length_15624_cov_4.205472, whole genome shotgun sequence.
ACCESSION   DAOMPG010000031 DAOMPG010000000
VERSION     DAOMPG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15624
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1645_length_15624_cov_4.205472"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLS34_02835"
     CDS             complement(<1..764)
                     /locus_tag="PLS34_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86443.1"
                     /translation="MRQQGRQPSRQSRRQGSPQRRRPRTGSDHGRIVARHIGERKHHH
                     PPAGAARREPAGLQQRQPGADPVDGRDRQPAGEQPVVGARQPGCIDPARQRLGQRGAA
                     AGQEAQQRHARRQGVDELQQFAPGQQAALAGHRMVAEQDPGVRHAGFAGPRGAGAGDA
                     QRRSTPGRERIRQAGQHGRRRLAEGQHGPVRTGPELGGRPRHHAPAVDGLERRREAPA
                     QQFAGVLRHRQAGRRRGRFPRYRISAVRPATTGRRAV"
     gene            877..1350
                     /locus_tag="PLS34_02840"
     CDS             877..1350
                     /locus_tag="PLS34_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HRP86444.1"
                     /translation="MRLALAAGAQAAGLAVQMHAAPLGGTTDQAAGEILAALSQGPPG
                     LHGWSGETTVRLPENPGAGGRNSHLALTLGRALDGRRDVVALCAATDGSDGTGDAAGG
                     WADGGLLERGRALGLDARAALAAADSGRYLAATGDALLTGPTGTNVMDLVLALRR"
     gene            1429..2640
                     /locus_tag="PLS34_02845"
     CDS             1429..2640
                     /locus_tag="PLS34_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRP86445.1"
                     /translation="MPQRILIVDDHRDYRTWLSHHLAAARPQAQVECHDPVGDDPLPA
                     DFEPARWDLVFLDHSLGEHDGLEVLRRLKAGPDCPPIVFLTPQGDQQTIVRALEAGAD
                     DFLSKGRSTHDHISRVVREALRRGQGLKARPGRARKLEQPTFRLKGHRFLKALGSGAT
                     ASVYLMERERNQRLVVAKVFRHVPDRVDQFLPLQRFLREYEVISGIRHPNVVEIFDLG
                     IADDMAFIVMEFFAGGHLGERLSRGMPAPQALEYLVQIARALDAIHAVGVLHRDLKPA
                     NIMLRDDGSLALIDFGVAKLRDSAAELTGLGEVFGTPYYMSPEQGEGAPVDERADIYS
                     VGVVFHEMLTGRKPYVAGSPMAIIWKHRHAPLPVLPAPLASYQPLLERMMAKDPAGRF
                     ESARALLDALP"
     gene            2675..3283
                     /locus_tag="PLS34_02850"
     CDS             2675..3283
                     /locus_tag="PLS34_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HRP86446.1"
                     /translation="MHAELNDFLGAATPEAWAEAAADPAVLPMLLVDHANCEKKAAST
                     ALAMLFRYEEHAVLCDRLSRLAREELRHFEQVRQIMRRRGIAWRRVPASRYAAGLAAA
                     VRKDEPGRLVDRLVVGAFIEARSCERFAMLAPRLDAELGRFYNGLLASEARHFRNYLA
                     LAEGFAGDDFGARIDAIRQVENALATEPDTELRFHSGPPVSG"
     gene            complement(3409..4320)
                     /locus_tag="PLS34_02855"
     CDS             complement(3409..4320)
                     /locus_tag="PLS34_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86447.1"
                     /translation="MPALSEHHLTERVDRFRDELRILVDQADSTNTRPDRDRIEGLYQ
                     KYFPNPRLIKTSTKHTLAELAALHAGIEDAFSYTLSPLMLGRLGQLVGQITEQLRQDV
                     QRGPDAALRYLTRFHADLLRARRFTQARDLVERYELNAPHWNLIDESPAGAGPAVLDI
                     AADRPPVTLTRRWVDLGPDPMLVAVVHPHCGPSRRAMMHIENHPDLAALFANRTVWLA
                     DARQVNLEPLTDWNAQARETKISISYDNYAWPEAIGFMRTPVFYFLRDAVVRDVVIGW
                     PGVEQVERLHAGFDAIGVDSGISPIAR"
     gene            complement(4489..5028)
                     /locus_tag="PLS34_02860"
     CDS             complement(4489..5028)
                     /locus_tag="PLS34_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86448.1"
                     /translation="MNLPYLALGVLCAISMTTASAEPHVQSHPTRGAHSIELDRSAIS
                     AAPVQSRADLESHLADNPDSAFHRLSHGGRETFLASLVLTEKGLGSFNYKVLEDELTP
                     TQIHEVLSLFGLGELVSHFKNARVETPHDAQLLRSDQSATSSTSCLVIENHYCSPPAT
                     CRYENGAHCITCNCGMDEP"
     gene            complement(5238..5960)
                     /locus_tag="PLS34_02865"
     CDS             complement(5238..5960)
                     /locus_tag="PLS34_02865"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRP86449.1"
                     /translation="MTTLFISDLHLDPARPDITGQALEFLATETQGAEALYILGDLFE
                     AWVGDDDPEHEKRRVIAALKQLTDGGLPCYFMHGNRDFLVGEGFAAATGCRLLADPTI
                     VELHGTKVMLMHGDTLCTDDHQYQAFRQMVRNPSWQQAMLARPLAERVALAKQMREAS
                     AASMAGKSEEIMDVNQDAVESAMREHGVYTLLHGHTHRPAVHRFELDGREAVRIVLGD
                     WYTQGSALSWHADGPVLRTLPR"
     gene            complement(5974..6549)
                     /locus_tag="PLS34_02870"
     CDS             complement(5974..6549)
                     /locus_tag="PLS34_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP86450.1"
                     /translation="MRPILSALALSLVAAPAGADDPGSKAPKVELVTTHGSLVLELDP
                     VNAPITTENFLRYVRDGHYDGLIFHRVIAGFMAQGGGYQADYSERETREPIKNESHNG
                     LSNERGTIAMARTSDPHSATAQFFINLVDNQRLDGAPGRWGYTVFGQLVEGIDVLDKI
                     GAVPTGSGGPFRSDVPQAPVVIQQARVIEAE"
     gene            6705..8096
                     /gene="gltX"
                     /locus_tag="PLS34_02875"
     CDS             6705..8096
                     /gene="gltX"
                     /locus_tag="PLS34_02875"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRP86451.1"
                     /translation="MSIVTRFAPSPTGRLHAGNLRTALFNWCLARGAAGRFLLRSEDT
                     DVAREGEGDLAHQLADLAWFGLDWDGGPDAPAPEGPFRQSERGELYREYLGRLAESGR
                     AYTCYRTEQELKQRRAEQRRRGLPPRYDREWAQLPHEEIVRRRQGGQMPVIRFAAPDT
                     GEIAWQDLVRGARSLPATEVADFVLSRSDGSPGFLFANAIDDALMGVTHVLRGEDHLV
                     NTARQLVILEALGLPAPLYGHMPLLSGPDGAKLSKRSGAVSVGGFRDAGVLPLALANT
                     LARLGHPWEEQGLLSMAELARGFDPAKLSSAAQRFDPGQIGHWQRLALDALPESELAA
                     WAGEAALAPVPVADRPALLAAVRHNVARPADVAEWAGLLFGAAELEPRPETGAAPALF
                     AAALAAWDAGATDHKALAGPVKEAVGASGKGFFRPLRMALTGRPDGPELGALFAAMPR
                     ATIRARLAQHAGH"
     gene            8108..9490
                     /gene="cysS"
                     /locus_tag="PLS34_02880"
     CDS             8108..9490
                     /gene="cysS"
                     /locus_tag="PLS34_02880"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRP86452.1"
                     /translation="MLHIHNSLTGAKEPFQPIEPGRVRMYACGMTVYDYCHIGHARAH
                     VVFDVVARYLRWSGYQVEFVRNITDIDDKIIKRAAERGETLQQLTQRYIDAMNEDFAA
                     LGILPADHEPRATEHMPGIIAMIEALVARGHAYAAGNGDVYYRVASFPEYGKLSGKRL
                     ADLRAGARVEVDEAKGDPLDFVLWKAAKPGEPAWDSPWGPGRPGWHIECSVMSTEILG
                     AHFDIHGGGLDLKFPHHENEIAQSCGATGAKFVNYWMHNGFVRVDEEKMSKSLGNFFT
                     IREVLERYPAEAIRYFVLSSHYRSPLNYTEQNLDQAEASVRRLYTALRDVEPGANVDP
                     AWVERFRAVMDDDFNTAEALALFQQLAGALNSAKAGGDAAGAATLAATLRTVAGVLGI
                     AQADADAWLKRGGETFPLCEHEIEARIEARMQARKRKDFAEADRIRDELAVAGIVLED
                     GPGGTTWRRG"
     gene            complement(9531..10796)
                     /locus_tag="PLS34_02885"
     CDS             complement(9531..10796)
                     /locus_tag="PLS34_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86453.1"
                     /translation="MRYRDACIVLLAWLGCGHVVLAGGPDEARARDQFAAGTQHFQAQ
                     DYDAALAAFRAAVASGLSGPAVHYNIGVSAWQLGRLDEAEAAFRIVAGYPAMATVARY
                     NLGLVALRRGHDDAARDWFEAAHAGDDDAIRHLAAAQLEALEPAPPSVEAAPRPVVFL
                     STRAGYDDNVVLAADGEVLGVSDTGSSVIEAQLAGLVPLAAGIRLAGSAFMLRYPQLG
                     EFDQAGGRVGLLARRNLGEWQGEIGVEYEFNRLDGRHFEDRIGLSATAYRSLPSDWDL
                     RLRVRHEDIDGRRPYASLSGDRQELDVRLRRQTAGQELRVGYRYERNDRAGAELSPDR
                     HTLEAEWELGLRDGVRAVLGTAWRHSRYAPAAGGWTERRSFVSAGVRGALAGAWTWTA
                     GYEWTDNDSADAWFDYRRQRIMVGVEALF"
     gene            complement(10892..11359)
                     /locus_tag="PLS34_02890"
     CDS             complement(10892..11359)
                     /locus_tag="PLS34_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86454.1"
                     /translation="MIGVPNSRAYRAASRSGTMGYRKIVSAIVLCSLGGLPLAVAEEQ
                     PPRRTGAAEGLEITMRIIEDPDAIAAEAITRRLELPTPRGARDSGPPPGPAEGAGKSE
                     AAGQQGKEFGAEVSERARELARQAGEQREEFGRSKAEEMRPDPPRPPAPPPRP"
     gene            complement(11362..12069)
                     /locus_tag="PLS34_02895"
     CDS             complement(11362..12069)
                     /locus_tag="PLS34_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRP86455.1"
                     /translation="MNDLDCTLVRRRLEDLLDGYLSATDSAAAELHLARCRGCTAERA
                     AIESLRGELKALPVPEPSPGFAAAALVAAARRNVPAAAAERGFQALPAGRSAPRRRLE
                     LWIGAALGAAATAVFMVLLSGLPQREPPPGIEAPSGIRLALHEVREIGVAIDVDAAVS
                     GAMMTVLLEGGVDLVGFGEQREISWRTDLDAGTNVLSLPIIAHSMEGGRLTALLRHGD
                     TTRRVDLSVSVAPPAVN"
     gene            complement(12066..12683)
                     /locus_tag="PLS34_02900"
     CDS             complement(12066..12683)
                     /locus_tag="PLS34_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRP86456.1"
                     /translation="MADRSVPRRREAGNSHQEFERLLQPLLPTLFRHAFRWTAARDQA
                     EDLVQELLVRLYPRLDELAALDRVQPWVLRVMYRIFVDQHRRNTNSPVRPAHEMPGMI
                     EDGEDDPAARCADQAPQPPELVERELDGRRLAAAWTALSEDHRVVVALHDIEGYRLEE
                     LSAVLEVPVGTLKSRLHRARAQLRRLLATEPLSTAARVAEGRGQQ"
     gene            12894..13844
                     /locus_tag="PLS34_02905"
     CDS             12894..13844
                     /locus_tag="PLS34_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRP86457.1"
                     /translation="MISAKNSLRVTLLAMATLIFAACGSDSKPGTGTLSMSVTDAPVD
                     EAHAVVIAMTEFEFKPVDGPSFRVPVIEPGRELNLLAFSNGEAALVIDGEEVPAGDYE
                     WLRIYFDEAATYVVLEEGGGTYPLFIPSGAQTGFKVVSGFTVPANESVAYMLDFDLRK
                     SLHSPPGLAGRFGEDRVFLLRPTVRILNMAETGGVWGVVADGLLAMNNDEASCAGGDA
                     VYAFEGLDVDPRGEGVLPLVSDIVELNETEGWHEYQLAYLLPGDYTLAFTCSASADDG
                     EPETYPLDDLLFSEVINVTVIEEQHKRCDIPPAAGQADPC"
     gene            complement(13936..14790)
                     /gene="folD"
                     /locus_tag="PLS34_02910"
     CDS             complement(13936..14790)
                     /gene="folD"
                     /locus_tag="PLS34_02910"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRP86458.1"
                     /translation="MTAQIIDGRQVAANLQREVRARVENRLAAGRRRPGLAVVMVGED
                     PASTIYVRNKRKACDDAGIRSVAYDLPADTGQEALDALIAGLNEDPAIDGILVQLPLP
                     AQLDARRVIEAIDPAKDVDGFHPYNIGRLAQRIPLLRPCTPAGVMQLLRHYEIPVRGR
                     HAVVIGASNIVGRPMALELLLAGATTTVCHRFTRDLAAELVRAEIVVVAVGKPGLVRG
                     EWLQPGAVVVDVGMNRLNDGRLCGDVEFEAAMERVAWITPVPGGVGTMTVAMLMKNTL
                     DAAERRDP"
     gene            14915..14991
                     /locus_tag="PLS34_02915"
     tRNA            14915..14991
                     /locus_tag="PLS34_02915"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14949..14951,aa:Pro,seq:tgg)
     gene            15381..15456
                     /locus_tag="PLS34_02920"
     tRNA            15381..15456
                     /locus_tag="PLS34_02920"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15414..15416,aa:His,seq:gtg)
     gene            15548..15623
                     /locus_tag="PLS34_02925"
     tRNA            15548..15623
                     /locus_tag="PLS34_02925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15581..15583,aa:Lys,seq:ctt)
BASE COUNT         2370 a         5310 c         5437 g         2507 t
ORIGIN      
        1 acggcgcgcc ggcccgtggt cgccggccgc acggcagaga tcctgtagcg cgggaatcgc
       61 ccgcgccgcc ggcctgcctg cctgtgccgc aagacccccg cgaactgctg cgccggtgcc
      121 tcgcggcggc gctcgaggcc gtcgacggcc ggcgcgtggt ggcgcgggcg gccgccgagt
      181 tcggggccgg tgcggactgg cccgtgctgg ccctcggcaa ggcggcgccg gccatgctgg
      241 ccggcctggc ggatgcgctc ccggcctggc gtggaccggc gctgtgcatc acccgcgccg
      301 gcaccgcgcg gcccggcgaa cccggcgtgg cgcacgcccg ggtcctgctc ggcgaccatc
      361 cggtgcccgg cgagcgcagc ctgctggccg ggcgcgaatt gctggagttc gtcgacgcct
      421 tgccgccggg cgtgccgctg ctgtgcctct tgtccggcgg cagctccgcg ctggccgagg
      481 cgctgccggg cggggtcgat gcagccgggc tggcgcgcgc caaccactgg ctgctcgcca
      541 gcgggctgcc gatcgcggcc gtcaaccggg tccgcgccgg gctgtcgctg ttgaaggccg
      601 gccggctcgc ggcgcgctgc gccggccggc gggtggtggt gcttgcgctc tccgatgtgc
      661 cgggcgacga tccggccgtg atcgcttccg gtccgtggac ggcgtcgctg tggcgacccc
      721 tgccggcggg attgccggga tggctgacgg ccctgctggc gcacgcgccg gcgccgccgg
      781 acggcgccgg cctggccgag gtcgactacc gggtgctcac gagcgggcgc cgcgccctgt
      841 cggccgcggc ggccgtcgcg caggaaggac gccgacgtgc ggctggcgct ggccgcgggc
      901 gcgcaggctg ccggcctcgc ggtgcaaatg cacgccgcgc cgctgggcgg caccacggac
      961 caggccgcgg gcgagattct cgccgcgctg tcgcagggcc cgccgggcct gcacggctgg
     1021 tcgggcgaga ccacggtgcg gctgccggag aacccaggcg cgggcggccg caacagtcac
     1081 ctggcgctga cgctcggccg tgcgctcgat ggacgtcgcg acgtggtcgc gctgtgtgcc
     1141 gcgaccgacg gcagcgacgg gaccggggac gctgccggcg gctgggccga cggcgggctg
     1201 ctcgagcgcg gccgggcgct cggactcgat gcgcgcgccg cgctggcggc ggccgattcg
     1261 ggccgctatc tcgccgccac cggcgatgcg ctgttgaccg ggccgaccgg cacaaacgtc
     1321 atggacctgg tgctggcgct gcgccggtga ggtctgcgat gcgcgtgatg ctggcccgcg
     1381 ggggatcggg atgtttatgc taccggctgc cgttcgcctg gagtgctcgt gccgcagcgc
     1441 attctcatcg ttgacgatca ccgcgattac cggacgtggc tgagtcatca cctcgccgcg
     1501 gcgcggccgc aggcgcaggt cgagtgccac gacccggtcg gcgacgatcc cttgccggcg
     1561 gacttcgagc ccgcgcgctg ggacctggtg ttcctcgacc acagcctggg tgagcacgac
     1621 ggcctggagg tgctcaggcg actgaaggcc gggcccgact gtccgccgat cgtgttcctc
     1681 acgccgcagg gcgaccagca gaccatcgtg cgcgcgctgg aggcgggcgc cgacgatttc
     1741 ctttccaagg gccgtagcac ccacgatcac atcagccggg tggtgcgtga ggcgctgcgc
     1801 cggggccagg gactcaaggc gcgcccgggc cgcgcgcgca agctcgagca gccgaccttc
     1861 cggctcaagg gccatcgctt cctgaaggcg ctgggcagcg gcgccaccgc ctcggtgtac
     1921 ctcatggagc gcgaacgcaa ccagcgcctg gtggtggcga aggtgttccg ccacgtgccc
     1981 gaccgcgtcg accagttcct gccgctgcag cgcttcctgc gcgagtacga ggtcatctcg
     2041 ggcattcgcc atcccaatgt cgtggagatt ttcgacctcg gcatcgccga cgacatggcc
     2101 ttcatcgtca tggagttctt cgcgggtggc catctcggcg aacgcctgtc acgcggcatg
     2161 cccgcgccgc aggcgctcga atacctggtg cagatcgcac gcgcgctcga cgccattcat
     2221 gcggtcggcg tgctgcatcg cgacctcaag ccggccaaca tcatgctgcg cgacgacggc
     2281 agcctggcgc tgatcgactt cggcgtcgcc aagctgcgcg actccgccgc cgagctgacc
     2341 ggcctcggcg aagtgttcgg cacgccctat tacatgagcc cggagcaggg cgaaggcgcg
     2401 ccggtcgacg agcgcgccga catctacagc gtgggcgtgg tgttccacga gatgctcacc
     2461 gggcgcaagc cctacgtcgc cggctcgccc atggcgatta tctggaagca ccggcatgcg
     2521 cccttgccgg tactgccggc gccgctcgcg tcctaccagc cgctgctcga gcggatgatg
     2581 gccaaggacc cggccggccg cttcgagtcc gcgcgcgccc tgctcgatgc cctgccgtga
     2641 aaatggggac attccccatt tcgggagacg ggtcgtgcac gcggagctga atgattttct
     2701 cggcgcggcg acgcccgagg cctgggcgga ggcggccgcg gacccggcgg tgttgccgat
     2761 gctgctggtg gaccacgcca actgcgagaa gaaggcggcg tccaccgcgt tggcgatgct
     2821 gtttcgctac gaggagcacg cggtgctgtg tgatcggctg tcgcggctgg cgcgcgagga
     2881 gttgcgccat ttcgagcagg tgcgccagat catgcgccgg cgcggcatcg cgtggcggcg
     2941 ggtgccggcg tcgcgctacg ccgccgggct ggccgcggcg gtgcgcaagg acgagccggg
     3001 ccgcctggtg gatcgcctcg tggtcggcgc tttcatcgag gcgcgttcat gtgagcgctt
     3061 cgcgatgctg gcgccgcggc tcgacgcgga gctcggccgt ttttacaacg ggctgctcgc
     3121 ttcggaggcg cggcactttc ggaattatct tgccctcgcc gagggattcg ccggcgacga
     3181 tttcggcgcc cgcatcgacg ccattcgcca ggtcgagaat gctcttgcca ctgagcccga
     3241 cacggagctt cgtttccaca gcgggccgcc tgtgtctggt tgaccgcgag ggcgcgccca
     3301 tggcgtctcg cccccgctac cgtgtcgatc gaggcggcgc tgaccgttgc ccacggggac
     3361 acaagtgatt gcgaggcgtc gttggtcggc gttgctgcgc ggagcttgtc agcgggcgat
     3421 cgggctaatg ccagagtcga ccccgatggc gtcaaagcca gcgtgaagac gctcgacctg
     3481 ctcgacgcct ggccagccga ttacgacgtc gcgcaccacg gcatcccgaa ggaaatagaa
     3541 cacgggtgtg cgcatgaatc cgatcgcctc aggccacgcg taattgtcat aggagatgga
     3601 tatcttggtc tctcgagcct gggcgttcca gtccgtaagt ggctcgagat tcacttggcg
     3661 tgcgtccgca agccacactg tccggttggc gaacagtgca gccaggtccg ggtggttttc
     3721 gatgtgcatc atggcgcgcc tggaaggtcc gcagtgcgga tgaaccaccg ccacaagcat
     3781 cgggtctggt ccaagatcca cccaccttcg cgtgagtgtc acaggaggcc ggtctgcggc
     3841 gatgtcgaga accgctggtc cagcgcctgc aggcgactcg tcgatcaagt tccagtgtgg
     3901 tgcattgagc tcgtatcttt ccaccagatc gcgggcctgg gtgaagcggc gcgcccggag
     3961 aaggtccgcg tggaatctcg tcaggtacct cagtgcggca tccgggcccc tttgaacgtc
     4021 ctgccgaagc tgctccgtaa tctgcccaac tagctggccg agtcggccca gcatgagtgg
     4081 cgacaaggtg tatgagaacg catcctcaat cccggcgtgc agggctgcaa gctcagccaa
     4141 tgtgtgtttc gttgaagtct tgatgagccg agggtttggg aaatatttct ggtataggcc
     4201 ttcgatccgg tcgcgatcgg gccgggtgtt ggtcgagtcc gcttggtcca ccagaatgcg
     4261 caattcatcg cgaaatcggt cgacgcgctc ggtgaggtga tgctcgctca gtgcaggcaa
     4321 gggacaaagc aaccccgccc atatccagag gatgatcgtc cgtaatgtgt acattatggg
     4381 tcagcgtgcc cggctgcgac ttggacgtcg cgaactccaa ggtcgccatc gggtttgggg
     4441 gatgccgcct gcatgcagtc aggggaactt gctgattatc cctattggct agggttcatc
     4501 catgccgcaa ttacaagtga tgcaatgcgc tccgttttcg tacctgcacg tagcaggtgg
     4561 agaacagtag tggttctcga taacgaggca tgaagtgctg cttgttgcag attggtcgga
     4621 cctcaacagc tgcgcatcat gcggagtttc gacgcgcgcg ttcttgaaat gggaaacgag
     4681 ttcaccgagt ccgaacagcg aaaggacttc gtgaatctga gtcggggtca gttcgtcctc
     4741 cagtacctta taattgaagc tgccgaggcc tttctcggtc aacaccaggc tcgcgagaaa
     4801 ggtttcgcga ccgccatggg acagccgatg aaacgccgag tcagggttgt cagcgaggtg
     4861 gctttcgagg tccgcacgag actggacagg agctgctgaa atcgctgaac gatctaactc
     4921 gatagagtga gcgccgcgcg ttggatggct ttggacatga ggttcagcag atgcggtggt
     4981 catggatatt gcacaaagaa cgccaagtgc aagataaggc agattcattg cagtttcctc
     5041 ctcgatagtc acatcctatt ctggttagta acaccagggt gagttggcag gatcattgcc
     5101 caagcacact taacaaagcc gccagcctgt gtcaaatttg atcgcagtcg aatggtgttt
     5161 tgtcgacgtc agtcatcagc gactgctaaa tgccgatcga acaacgagag aaagacttgc
     5221 ccgcagcctc gggtgggcta gcgcggcagc gtcctgagca cggggccatc ggcatgccag
     5281 ctgagcgcgc tgccctgcgt gtaccagtcg cccagcacga tccgcacggc ttcgcggccg
     5341 tcgagctcga agcggtgcac cgccggtcga tgagtgtggc cgtgcagcag cgtgtagacg
     5401 ccgtgctcgc gcatcgccga ctcgaccgcg tcctggttga cgtccatgat ctcttcggac
     5461 ttgccggcca tggacgccgc actggcctcg cgcatctgct tcgccaatgc cacccgctca
     5521 gcaagcggtc gcgccagcat cgcctgttgc caggacgggt tgcgcaccat ctggcggaag
     5581 gcctggtact ggtggtcgtc ggtgcacagc gtgtcgccgt gcatcagcat gaccttcgtg
     5641 ccgtgcagct cgacgatggt cgggtcggcc agcagcctgc agccggtggc ggcggcgaag
     5701 ccctcgccga caagaaaatc ccggttgccg tgcatgaagt aacacggcaa tccgccatcg
     5761 gtaagttgtt tgagcgccgc gatgacgcgg cgcttctcgt gctccgggtc gtcatcgccg
     5821 acccacgcct cgaacaggtc gccgaggatg tacaacgcct cggcgccctg cgtctcggtg
     5881 gcgagaaact ccagcgcctg gccggtgatg tcgggccgcg ccgggtcgag gtgcaggtcg
     5941 gagatgaaca gcgtcgtcac tggccgggtc cgctcattcc gcctcgatga cgcgcgcttg
     6001 ctggatcacg accggcgcct gcggcacgtc gctgcggaac gggccgccgc tgccggtcgg
     6061 cacggcgccg atcttgtcga gcacgtcgat gccctcgacc agctggccga acacggtgta
     6121 cccccagcgg ccgggcgcgc cgtcgaggcg ctggttgtcc accaggttga tgaaaaactg
     6181 cgcggtggcg gagtgcgggt cgctggtgcg ggccatggcg atggtgccgc gctcgttgga
     6241 gaggccgttg tgcgactcgt tcttgatcgg ttcgcgcgtc tcgcgctccg agtagtcggc
     6301 ctggtagccg ccgccctggg ccatgaagcc ggcgatgacg cggtggaaga tcagcccgtc
     6361 gtagtggccg tcacgcacat agcgcaggaa gttctcggtg gtgatcggcg cattgaccgg
     6421 gtcgagctcg agcaccagcg agccgtgcgt ggtgaccagc tcgaccttgg gggccttcga
     6481 gccgggatcg tcggcgccgg ccggcgcggc cacgagcgaa agcgcgagtg cagagagaat
     6541 cgggcgcatg ggaagctcct cggtgacaga ggcgggaaca ttagtcgagc ccgcgtgcgc
     6601 gcgcaaaccg gaattgggga cattccccaa ttcgcgcgaa ttggggaatg tccccaattc
     6661 cgggcatgtc gcggtttcac gcagtccgtt accattacaa gcttatgtcc atcgtcaccc
     6721 gcttcgcgcc gagcccgacc ggccgcctgc acgccggcaa cctgcgcacc gcgctgttca
     6781 actggtgcct ggcgcgcggt gcggcggggc gatttctcct gcgctccgag gacaccgacg
     6841 tcgcgcgcga gggcgagggc gacctggcgc accagctggc cgacctggcg tggttcgggc
     6901 tcgattggga cggcgggccg gatgcgccgg cgcccgaggg cccctttcgc cagtcggagc
     6961 gcggcgagct ctaccgcgag tacctgggcc ggctggccga gtccggacgc gcctacacct
     7021 gctatcgcac cgagcaggag ctgaagcagc gccgcgccga gcagcgccgc cgcggcctgc
     7081 cgccgcgcta cgaccgcgaa tgggcgcagt tgccgcacga ggagatcgtg cgccgccgcc
     7141 agggtggcca gatgcccgtg atccgctttg ccgcgccgga caccggcgag atcgcctggc
     7201 aggacctggt gaggggagcg cggtcgctgc cggccaccga agtggccgat ttcgtgctga
     7261 gccgcagcga cggctcgccg ggcttcctgt tcgccaacgc catcgacgat gcgttgatgg
     7321 gcgtgacgca tgtgctgcgc ggcgaggacc acctggtgaa caccgcgcgc cagctggtga
     7381 tcctcgaggc gctcggcctg cccgcgcctc tctacggcca catgccgctg ctgtcggggc
     7441 cggacggcgc caagctgtcc aagcgcagcg gcgcggtcag cgtcggcggc ttccgcgacg
     7501 cgggcgtgct gccgctggcg ctggccaaca cgctggcgcg gctgggccat ccgtgggagg
     7561 aacagggcct gctgtccatg gcggagctcg cccgcggctt cgatcccgcc aagctcagca
     7621 gcgccgcgca gcgcttcgac ccgggccaga tcggccactg gcagcggctc gcactggacg
     7681 cgctgccgga gagcgagctg gccgcatggg ccggagaagc cgcgttggcg cccgtgccgg
     7741 tggccgaccg gccggccttg ctggccgcgg tgcggcacaa cgtcgcgcgc ccggccgacg
     7801 tggcggagtg ggccgggctg ctgttcggcg ccgccgagct cgagccgcgg cccgagaccg
     7861 gcgcggcccc ggcgttgttc gcggccgcgc tggcggcctg ggatgccggc gcgaccgacc
     7921 acaaggcgct ggccgggccg gtcaaggagg ccgttggcgc ctccggtaaa ggatttttca
     7981 ggccgctgcg catggcgctg accggccggc ccgacggccc cgagctcggc gcgctgttcg
     8041 cggcgatgcc gcgcgctacc attcgtgcgc gcctcgcgca gcacgccggc cactgacagg
     8101 gaacgccatg ctccacattc acaattctct gaccggcgcc aaggagccct tccagcccat
     8161 cgagcccggc cgggtgcgca tgtacgcctg cggcatgacg gtgtacgact actgccacat
     8221 cggtcatgcg cgcgcccacg tcgtgttcga cgtggtggca cgttacctgc gctggtccgg
     8281 ctaccaggtc gagttcgtcc gcaacatcac cgacatcgac gacaagatca tcaagcgcgc
     8341 ggcggaacgc ggcgagacgc tgcagcaact gacgcagcgc tacatcgacg ccatgaacga
     8401 ggatttcgcg gcgctcggca tcctgccggc cgaccatgag ccgcgcgcca ccgagcacat
     8461 gcccggcatc atcgccatga tcgaggcgtt ggtcgcgcgc gggcatgcct acgcggcagg
     8521 caacggcgac gtgtattacc gcgtcgccag ctttcccgag tacggcaagt tgtcgggcaa
     8581 gcggctcgcc gacctgcgcg ccggcgcgcg cgtcgaggtc gacgaggcca agggcgaccc
     8641 gctggacttc gtgctctgga aggccgccaa gccgggcgag ccggcgtggg actcgccgtg
     8701 gggcccgggg cggcccggct ggcacatcga gtgctcggtg atgtccaccg agatcctcgg
     8761 cgcgcatttc gacatccacg gcggcggcct cgacctgaag ttcccgcacc acgagaacga
     8821 gatcgcgcag agttgcggcg ccaccggcgc gaagttcgtc aattactgga tgcacaacgg
     8881 cttcgtgcgc gtggatgaag agaagatgtc caagtcgctg ggcaacttct tcaccatccg
     8941 cgaggtgctg gagcgctacc cggccgaggc gatccgttac ttcgtgctgt cgagccatta
     9001 ccgcagcccg ctcaactaca ccgagcagaa tctcgaccag gcggaggcct cggtgcggcg
     9061 cctctacacg gcgttgcgtg acgtcgagcc cggcgcgaat gtcgatccgg cctgggtcga
     9121 gcgcttccgc gcggtgatgg acgacgattt caacaccgcc gaggcgctgg cgctgttcca
     9181 gcagctcgcc ggcgcgctca attcggccaa ggcgggcggc gacgcggccg gcgccgcgac
     9241 gctcgccgcg accttgcgca cggtcgccgg cgtgctcggc atcgcgcagg ccgatgcgga
     9301 cgcctggctg aagcgcggcg gcgaaacgtt cccgctgtgc gagcacgaga tcgaggcgcg
     9361 catcgaggcg cgcatgcagg cgcgcaagcg caaggacttt gcggaggcgg atcgcattcg
     9421 cgatgagctc gcggtcgcgg gcatcgtgct ggaggacggg ccgggcggca cgacctggcg
     9481 acgcggctag cgcggtcgcg gacggcgccg ctccggccgt cgatctgggc tcagaacagt
     9541 gcttcgacgc cgaccatgat ccgttgccgc cggtaatcga accacgcgtc ggcggagtcg
     9601 ttgtccgtcc actcgtagcc ggcggtccag gtccaggcgc ctgccagcgc gccgcgcacc
     9661 cccgcggaga cgaagctgcg gcgttcggtc cacccgccgg cagccggtgc gtagcggctg
     9721 tgtcgccacg ccgttcccag tacggcccgg accccgtcgc gcaaaccgag ctcccattcc
     9781 gcctccaggg tatgcctgtc cggggacaac tcggccccgg cgcgatcgtt gcgctcgtag
     9841 cgatagccga cacgcagctc ctgcccggcc gtctgccgtc gcagcctgac gtccagctcc
     9901 tggcgatcgc cgctcagtga agcgtagggc cggcggccgt cgatgtcctc gtggcgaacg
     9961 cgcagccgca ggtcccagtc ggatggcaac gagcggtacg cggtcgccga cagcccgatg
    10021 cggtcttcga aatgccggcc gtcgagccgg ttgaactcgt actccactcc gatctcgccc
    10081 tgccattcgc cgagatttcg ccgggccagc aagccgaccc gcccgcccgc ctggtcgaac
    10141 tcaccgagtt gcggatagcg cagcatgaaa gcgctgcccg ccagccggat gcctgcggcc
    10201 aatggcacga gccccgccag ctgggcttca atgacactgc tgccggtatc gctgacgccg
    10261 aggacttcgc catcggccgc cagcacgacg ttgtcgtcgt agccggcccg ggtggacagg
    10321 aatacgaccg ggcgcggcgc tgcctcgacg gacggcgggg ccggttccag cgcctccagt
    10381 tgcgcagccg caaggtgccg gatggcatcg tcgtcgccgg cgtgcgcggc ttcgaaccag
    10441 tcccgggccg cgtcgtcgtg accgcggcgc aacgcgacca gtccgaggtt gtagcgcgca
    10501 acggtagcca tggccggata gccggcgacg atcctgaagg ccgcctcggc ttcatcgaga
    10561 cgcccgagct gccatgcgct cacgccgatg ttgtagtgca cggccggccc cgacaacccg
    10621 gacgccacgg cagcccggaa cgcagcaagc gccgcgtcgt agtcctgtgc ctggaagtgc
    10681 tgcgtaccgg ctgcgaactg gtctcgtgcc cgcgcttcgt cgggcccgcc ggccagcacg
    10741 acgtggccgc aaccgagcca ggcgagcagc acgatgcagg cgtcgcggta tctcaaagcc
    10801 gatccctggt cgagaatcga ctcaagaaga tagcaggttc cggcagtaca gggcccggct
    10861 cagcgcagcc gactgcaacc aggatccttt ctcaggggcg cggcggcggc gcaggcggcc
    10921 gcggtggatc gggccgcatt tcctcggcct tcgagcgtcc gaactcctcg cgctgctcgc
    10981 cggcctgccg ggcgagttcc cgcgcccgtt ccgagacttc cgcgccgaat tccttgccct
    11041 gctgcccggc ggcctcgctc ttgcctgcac cctccgccgg cccgggcggt ggcccgctgt
    11101 cgcgcgcccc gcgcggcgtt ggcagctcca gccggcgcgt aatcgcttcg gccgcaatgg
    11161 cgtcggggtc ttcgatgatc cgcatggtga tctcgagacc ttcggcggct ccggtgcggc
    11221 gcggcggctg ctcttcggcc acggcgagcg gcagcccgcc cagcgagcag aggacgattg
    11281 ccgaaacgat cttgcggtaa cccatggtcc cggacctcga cgcggcgcga tacgcacggc
    11341 tgtttggtac gccgatcatt ttcagttcac cgcgggcggc gccacgctga cgctcaagtc
    11401 gactcgccgg gtcgtgtcgc cgtgccggag caacgcagtc agccgaccgc cttccatgga
    11461 atgcgcgatg atcggcaaag atagcacgtt ggtgcctgca tccaggtccg tccgccagct
    11521 gatctcgcgt tgttcgccga agcccacgag gtcgacgccg ccctccagca ggaccgtcat
    11581 catggcgccg gagaccgcag cgtcgacgtc gatcgccacg ccgatctccc gcacctcgtg
    11641 caatgccagg cggatgccgg agggagcctc gattccgggt ggcggctcgc gttgcggcag
    11701 gcccgacagc agcaccatga agacggccgt ggcggcagca ccgagcgccg cgccaatcca
    11761 gagctcgagt cgccgccgcg gcgccgaccg acctgctggc aacgcctgga agccgcgctc
    11821 cgctgccgcg gccggcacgt ttcgccgggc cgccgcaacg agcgccgcgg ccgcgaagcc
    11881 gggcgatggc tccgggaccg gcagggcctt caattccccg cgcagcgact cgatggccgc
    11941 gcgctccgcg gtgcagccac ggcaacgggc gagatgcagt tcggcggccg cgctgtccgt
    12001 ggcggacagg taaccgtcga gcaggtcctc gaggcgtcgc cgcaccagtg tgcaatccag
    12061 gtcgttcatt gctgtcctcg tccctcggca acacgagcgg cggtgctcaa aggttccgtg
    12121 gcgagcagcc ggcgcagctg cgccctggcc cgatgcagtc gcgacttgag cgtgccgacg
    12181 ggcacttcga gcacggcgga cagctcctcc agccgatacc cctcgatgtc gtgcagcgcg
    12241 accaccaccc ggtggtcctc gctcagcgcc gtccaggccg ccgccaggcg acgcccgtcg
    12301 agttcgcgct cgaccagctc gggcggctgc ggcgcctggt ccgcacagcg tgcagcggga
    12361 tcatcctcgc cgtcctcgat catgcccggc atctcgtgtg ccggtcgcac cggcgagttc
    12421 gtgttgcggc gatgctggtc gacgaagatc cggtacatca cgcgcaacac ccagggctgg
    12481 acccggtcca gcgccgccag ttcgtcgagg cggggataca gccgcaccag cagttcctgc
    12541 accaggtcct cggcctggtc tcgcgccgcg gtccagcgga aggcgtggcg aaacagcgtc
    12601 ggcaacagcg gctgcagcag gcgctcgaac tcctggtggg aattcccggc ctcccgtcgc
    12661 cgcggtactg atcgatctgc catcaggaac acacacgcgc gggccgggac aatggttcca
    12721 gcggccgctc ggaacggcgt cgcgcggctg cagcgcgcgg cgcgggatgg aaccctatgg
    12781 gcgccccact cgtgtcatcc acagggccga ggctcggtgg ccgcgcgcgc gatccggcgg
    12841 ccgcacggcc gcagtgaccc ggtatgaaca ccagacaaat tgggagtgca cgcatgatca
    12901 gtgcaaagaa cagcctccgg gtgacgttgc tcgccatggc caccctgata ttcgcggcct
    12961 gcggcagcga cagcaagccc ggcaccggca cgctcagcat gtcggtcacc gacgcgcccg
    13021 tcgacgaggc ccatgcggtc gtcatcgcga tgaccgagtt cgagttcaag ccggttgacg
    13081 ggccctcgtt ccgcgtaccg gtcatcgagc cggggaggga gctcaacctg ctggccttca
    13141 gcaacggcga ggccgccctg gtcatcgacg gcgaggaagt gccggccggc gactacgagt
    13201 ggctgcggat ctacttcgac gaggccgcga cctacgtcgt tctcgaggag ggcggcggca
    13261 cctatcccct gttcattccc agcggggcgc agaccggctt caaggtggtg agcggattca
    13321 cggtcccggc caacgagtcc gtcgcgtaca tgctcgactt cgacctgcgc aagtcgctcc
    13381 actcgccgcc cggcctcgcc ggccgtttcg gcgaggaccg cgtgttcctg ctgcggccga
    13441 cggtgcgcat cctgaacatg gcggagaccg gcggagtgtg gggcgtggtg gccgacgggc
    13501 tgctggcgat gaacaacgac gaggcgagct gtgcgggcgg cgacgccgtg tacgccttcg
    13561 agggcctgga cgtcgatccg cgcggcgagg gcgtgctgcc gctggtcagc gacatcgtcg
    13621 aactgaacga gaccgagggc tggcacgaat accagctcgc ctaccttctg ccgggcgact
    13681 acacgctcgc gttcacttgc tcggcgagtg ccgatgacgg cgagccggaa acctatccgc
    13741 tcgatgacct gctgttcagc gaggtgatca acgtcaccgt gatcgaggaa cagcacaagc
    13801 gctgcgacat cccgccggca gcaggccagg cggatccctg ctgagcgttg cccgccgcca
    13861 ggggcctgat ttcctgaagg gccgctgccc tgtcatcctc cccccctttc gggggcagcg
    13921 gccctctttc caagcctagg gatctcgccg ttccgcggcg tccagcgtgt ttttcatcag
    13981 catcgccacg gtcatcgtgc ccacgccgcc cggcaccggc gtgatccacg cgacgcgctc
    14041 catggcggcc tcgaactcga catcgccgca cagccgcccg tcgttcagcc ggttcatgcc
    14101 gacgtccacg accacggcgc ccggctgcag ccactcgccg cgcaccagcc ccggcttgcc
    14161 gaccgccacc accacgatct cggcgcggac cagctcggcg gccaggtcgc gcgtgaagcg
    14221 gtggcagacg gtggtggtgg cgcccgccag cagcagctcc agcgccatcg gccgaccgac
    14281 gatgttcgag gcgccgatca ccacggcatg gcggccacgc accggaatct cgtagtggcg
    14341 cagcagctgc atgacgccgg ccggcgtgca gggccgcagc aggggaatgc gctgggcgag
    14401 gcggccgatg ttgtaggggt ggaagccgtc cacgtccttc gccgggtcga tggcctcgat
    14461 gacacggcgg gcgtccagct gggcgggcag cggcagctgc acgaggatgc cgtcgatggc
    14521 cgggtcctcg ttgaggccgg cgatcaacgc gtcgagcgct tcctggccgg tgtctgccgg
    14581 caggtcatag gccaccgagc ggatccccgc atcgtcgcag gccttgcgct tgttgcgcac
    14641 gtagatggtg gacgccggat cctcaccgac catgaccacc gcgagtcccg ggcgccgccg
    14701 gccggcagcc aggcggttct cgacccgcgc cctgacttcc cgctggaggt tggcggcgac
    14761 ctgccgcccg tcgatgattt gtgccgtcac gcgcctcggc cccggctgcg gaggggccgg
    14821 gattgtctca cgcaagcatc ggagcgaaca agaaaaaccg gggtttttcg cgttgacggc
    14881 tccgcggccc cggcgtatta tccgcagccc ttgccggggc atagcgcagg ctggtagcgc
    14941 gtctgctttg ggagcagaag gtcgggggtt cgaatccctc tgccccgacc agatttcgtg
    15001 tgagggggtt gattagcctg gtcaagcgac gcgcccgtag ctcaaccgga tagagcatcg
    15061 gcctttgagt gaggagcctg caacgggaaa cagaaccgct gcagtggacc gcaccataac
    15121 ggggaaacct taacaggtag tgctgatggc aatcccgtgg aaacctgccg attgcccgtg
    15181 caatctgcgg ggctccgtag agactagacg tgcggcgcct gagatggcga agggatagtc
    15241 cagaccacaa acctgccggg tggcggggcc gcgaaagcgg tagttggtaa gctaagccga
    15301 cggttgcagg ttcgagtcct gccgggcgcg ccaattccga gattcggggt tacaattccc
    15361 cgccttgtac ctcagttatg gtggacgtag ctcagttggt agagccccgg gttgtgatcc
    15421 cggtcgtcgt gggttcgagt cccatcgtcc accccacctt tccggttggc cgccgcggca
    15481 aagctttaca aaggccggct gcatcgccag aattccgccc ctgcccggca ggtgcagcgc
    15541 acttcacggg ccgctagctc aattggcaga gcaacggact cttaatccgc aggttgtagg
    15601 ttcgattcct acgcggccca ccaa
//
LOCUS       DAOMPG010000032        15571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1652_length_15571_cov_4.654357, whole genome shotgun sequence.
ACCESSION   DAOMPG010000032 DAOMPG010000000
VERSION     DAOMPG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_15571_cov_4.654357"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLS34_02930"
     CDS             complement(<1..848)
                     /locus_tag="PLS34_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP86459.1"
                     /translation="MKCHRLPLLFAALAMLAASPAQAVSLLDAYRDALRSDPTLREAA
                     ANRLAALEARPQARAALLPQVEGTGSYEYQYSSGTQTFPQRLDSGEVITVTSGFEQDT
                     EPRRAWQLRLTQTLFRWDQWVALRQADKQLARAEAEFRAAEQDLMARVAQRYFDVLGA
                     RATLQAAEAAKEAIGRQLEQAEKRFEVGLSAITDVQEAQASFDSAAAAVIEAKRTLAV
                     AGEFLREITGEYYEGLADAGPDTPLVSPEPLSVDDWVGTALQRNLSLEAARLGAEISR
                     DQIEANR"
     gene            complement(945..1265)
                     /locus_tag="PLS34_02935"
     CDS             complement(945..1265)
                     /locus_tag="PLS34_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRP86460.1"
                     /translation="MPREMSPKEFALRARTDPPPVLLDVREDWELAIAQLGDAVHIPM
                     GEVPSRLGELDPEREIVVMCRSGGRSATVARYLEQQGFGKVWNLGGGILAWSEQLDPS
                     IAQY"
     gene            complement(1265..1924)
                     /locus_tag="PLS34_02940"
     CDS             complement(1265..1924)
                     /locus_tag="PLS34_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRP86461.1"
                     /translation="MNLDAARHQMIGQQLRAWDVLDGRVLDVMGALPREQFLPLELRA
                     LAYADTDLPLGPGRRLAAPKLQGRALQALLPQPTDEALEIGSGSGYLTACLAALAGQV
                     TSTESDSAMAVHSRDNLAAVGVGNATVLEGDGLSMEFPGRFDVICVNGSLPDDAGMLD
                     RLRRLLKMGGRLFVVTGRPPIMQARRILRTGESEWSDATVFETCLPPLIDAPHTPEFV
                     F"
     gene            2108..4000
                     /gene="thiC"
                     /locus_tag="PLS34_02945"
     CDS             2108..4000
                     /gene="thiC"
                     /locus_tag="PLS34_02945"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRP86462.1"
                     /translation="MSANPRDELRDPARLGDAVTRPFAGSTKLHVAGSRPDIRVAMRE
                     IRQAPTPGIRGAEPNPPIVVYDTSGPYTDPDYHVDLLAGLPALRAAWVEARGDSAPLD
                     GQSSRFARARAGNPDAAALAFPARRVARRALAGSNVTQMHYARRGIVTPEMEYVALRE
                     NAGLEARREAWAAAGLLARHPGEAFGARLPELVTPEFVRAEVAAGRAIIPCNLNHPEL
                     EPMIIGRNFRVKVNANIGNSAVTSSIAEEVEKLVWATRWGADTVMDLSTGKHIHETRE
                     WIIRNSPVPIGTVPIYQALEKVDGRAEELNWELFRDTLVEQAEQGVDYFTIHAGVRLP
                     FIPLTAGRVTGIVSRGGSIMAKWCLAHHQENFLYTRFAEICEIMAAYDVAFSLGDGLR
                     PGSIADANDRAQFAELETLGELTRKAWEHEVQVMIEGPGHVPMQLIRENMDKELAACM
                     EAPFYTLGPLTTDIAPGYDHITSAIGAAQIGWYGTAMLCYVTPKEHLGLPDRKDVREG
                     MIAYRIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLGLDPDRAREYHDQTMP
                     REAHKLAHFCSMCGPQFCSMKITQEVRDYARTHGLDDPAAAIRSGMEEKSIEFRADAA
                     QVYRRG"
     gene            4023..6263
                     /locus_tag="PLS34_02950"
     CDS             4023..6263
                     /locus_tag="PLS34_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP86463.1"
                     /translation="MAVIETLTIGVAAGVLGLLGVIIWRRKAAAHQGGGAVPMRMSEV
                     RERAAPRADARAAPYAHEQSQPLSVLADQPMFLRLAERVDDAVLLHDDEIHYANAAAE
                     ALLAAGEPLAGRRLVDLVQPEHLEGLQAWLKARRAGRQSAVRLRLRDGNDLPIECELA
                     GFPLPAGSRIVGTVVRATRDELLSSARQQGTRVAEATLESISEGVITTDMRGHVDYLN
                     SAAEALLGCQRDAVHGRPFGEVVTLVDEGDRRPLGDPVMRCLEERRRVDLGRRALLIS
                     RASGNEFSVELRASPIRAGGQELSGCVVVLHDVSEIRGLTRQMSYQASHDPLTGLVNR
                     REFERRLEEGLKTARSGNGGHVLCYLDLDRFKAVNDTSGHVAGDNMLREIAGVLKDKI
                     RDSDVVARVGGDEFGMLLIGCPLEKARQIADDVCAAIRDYRFVWRDKIFNVGVSVGLV
                     QIGPESGSIEDTLSAADSACYIAKQQGRGRIHVYSSRDEAAARQRGEIVWLQRLQAAL
                     KENRFELYTQPIVALAGRPDTGPAFEVLLRLKDADGESQPMDFMQAAERYHLMPSVDR
                     WVVQATFAAIAAGALKLPSGRSAAINISSQTLADPQFLEFVVECLDRSGVRPEQVCFE
                     LSESVVAASMSHTARFIAVLHGLGCRFTLDDFGSGLGSFANLKQLALDYLKIDGSFIR
                     GLGNDEVDVAMVTAMIELARTLGIKVIAEHVETERALELVRALKVDFVQGYSVGRPQP
                     LGSARA"
     gene            6424..>8184
                     /locus_tag="PLS34_02955"
     CDS             6424..>8184
                     /locus_tag="PLS34_02955"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRP86464.1"
                     /translation="MLIEYGLVAALGCAIVAILYGAWSVRWVLAQSAGTPRMQEIAGA
                     IQEGAQAYLNRQYTTIGIVGVILFLVVGLALDWLTAGGFAIGAILSAATGYIGMFVSV
                     RANVRTAEAARRGLSPALDVAFRGGAVTGILVVGLGMLGTAGYYWILLATGHEDHAVH
                     ALIGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYAVTIIATMLLAVVMATEVGPNGVLYPLVLGGASILASVVGTF
                     FVKISGSGKIMNALYKGVIVSGVLAAVAFYPVTTSMMGDSPLGATNLFLCGVVGLVLT
                     AAMVVITEYYTATEYSPVRSVAAASETGHATNIIAGLAVSMKSTALPVLAVCIAIWTT
                     HSLGGLYGIAIAATAMLSMAGMVVALDAYGPITDNAGGIAEMSDLPSEVRDITDPLDA
                     VGNTTKAVTKGYAIGSAGLAALVLFADYTHGLEAAGKATEFLLDSPMTIIGLFIGGLI
                     PFLFAAMAMEAVGRAAGSVVNEVRRQFREIPGIMESTARPDYSRAVDMLTKAAIKEML
                     VPSLLPVAAPIVVGFLLGPEA"
     assembly_gap    8185..8241
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8242..8428
                     /locus_tag="PLS34_02960"
     CDS             <8242..8428
                     /locus_tag="PLS34_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRP86465.1"
                     /translation="AWDNAKKYIEDGNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAIN
                     PLIKIINIVALLLVPLL"
     gene            complement(8524..9780)
                     /locus_tag="PLS34_02965"
     CDS             complement(8524..9780)
                     /locus_tag="PLS34_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRP86466.1"
                     /translation="MKKKRNLFYAQSGGVTAVINATACGVIETARARRDRIGKVYAGR
                     MGIVGALREELIDTGKESAKDIAALRHTPGGAFGSVRYKLKSIEQSAAEYQRLIEVFR
                     AHDIGYFIYNGGGDSMDTANKVAQVARQMDYEITCVGVPKTVDNDLPVTDNCPGFGSV
                     AKYVAVSTLEAGIDVGSMPQTKVFILEVMGRHAGWIAAAGGLAGKDRSEPPHIILFPE
                     VPFDEERFVEMVRKSVEQHDSCVIVASEGIRRPDGSFVAESGTRDAFGHAQLGGVAPH
                     LAEIVRGRLGYKFHWAVADYLQRSARHIASKVDVEQAYAVGKAAVKMALAGVHGKLPV
                     IVRSSDQPYRWKVGEADLSAVANVEKKLPKKYITEDGFHITKACRRYLEPLIKGEDYP
                     PYRAGLPVYVKLKNKLAKKKLPDWTG"
     gene            9987..10631
                     /locus_tag="PLS34_02970"
     CDS             9987..10631
                     /locus_tag="PLS34_02970"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRP86467.1"
                     /translation="MRIVLLGAPGSGKGTQAKKLVEDFGYPQVSTGDLLRDAVARSTA
                     HGLKAKAAMDAGKLVSDDIVLGILRERVAQPDAARGFILDGFPRNLAQAEALEEVLAE
                     LGQPLDAAVLMDLDHAILLKRLTGRRSCTGCGRVYNIYFLPPPADGRCGSCGGELVQR
                     SDVKEETIGRRLEVYRAETEPLVDYYRQLGKLRTVDADAPFDEVYARLKDALGL"
     gene            complement(10658..11755)
                     /gene="rnd"
                     /locus_tag="PLS34_02975"
     CDS             complement(10658..11755)
                     /gene="rnd"
                     /locus_tag="PLS34_02975"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRP86468.1"
                     /translation="MSDYEFIDKAADAASLAREWAGVRELAVDTEFVRESTYRARLCL
                     MQVSDGERVACLDMLALDGPGPFADLLLDPTVRKVFHSARQDLEVLNEHLGQVPAPIW
                     DTQIAAAMLGHPDQVGYTQLTGAELGVTLPKDHARTDWGRRPLSDEQLRYAAADVRWL
                     LPLAARLDQALEQLGRGEWADAECSVLCDPALYAFDAAGAWRRVKGAGQLEGAALARA
                     AALAEWRELEARRADRPRRWIIRDEAIVALATRAPRDPAELEQVEGLPPALLRRHGET
                     LLRIARDADTAPPPERPAAGRLEPAEERRARRLMDRLREIARNQRISAPLLATRRDIE
                     ALVQGQRDLPLLHGWRRELAGEELLRMLATD"
     gene            11848..12384
                     /locus_tag="PLS34_02980"
     CDS             11848..12384
                     /locus_tag="PLS34_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86469.1"
                     /translation="MQALIRLFIEVALHRRGPQDVPTSGAVFGLALFAYLAIGAAVLW
                     PSAAGTEALLGQLALDVALLLLVFGGLLAGTGRAGRLRQTLTALLGTGALLSAVALPF
                     VWLAARALGNGAPVPGMELPALVSTTALFMLLLASLLVTGHILRHAMDWSYAAGVLTA
                     VLYFALSVAMFRRYFPVA"
     gene            12384..13769
                     /gene="mpl"
                     /locus_tag="PLS34_02985"
     CDS             12384..13769
                     /gene="mpl"
                     /locus_tag="PLS34_02985"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HRP86470.1"
                     /translation="MHLHILGIGGTFMAGIAALARAQGHRVTGSDRALYPPMSTQLAA
                     LEIELIEGYDNDRLDPAPDCVVVGNVLRRGLPVVERLLDSGLPYCSGPEWLAREVLRD
                     RWTLAVAGTHGKTTTTSILAFLLESAGLRPGFLVGGIPADFGVSARLGEAPFFVVEAD
                     EYDTAFFDKRSKFVHYRPRTLILGNLEFDHADIFPDLAAIQRQFHHLVRTVPGTGLIV
                     SKAGDAALEEVLAQGCWTPVERFRGPGEATPDAGHAARLVPGGFEVLAPDGASLATVH
                     WAMPGAHNAENALAAMLAARHAGVPMEVSARALANFRGVKRRQEWIGTVDGVELFDDF
                     AHHPTAIARTLEAMRDGLSAGRLVAVLEPRSNTMRMGVHAGSLARSLAGADRVFVHAP
                     ADLGWDAAAALAALGDEARVCAELAELETILLEELRAGDRVVLMSNGSFGGLHGRLRD
                     GLEARARRATE"
     gene            13766..14386
                     /locus_tag="PLS34_02990"
     CDS             13766..14386
                     /locus_tag="PLS34_02990"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HRP86471.1"
                     /translation="MSMHVALAMTGASGAQYGLRLLECLLGAGCRVTLMISRPAQVVI
                     GMETDLDLPARPAEIRRFFCARHGVGESRLAVHGREEWTAPVASGSAAPDAMVVCPCT
                     TATVSALATGASRSLIERAGDVVLKERRRLVLVVRETPFSPIHLENMLTLARAGAVIL
                     PANPGFYHRPAQVADLVDFVVARVLDHLGIAQSLVAPWGGTQDADE"
     gene            14383..14967
                     /locus_tag="PLS34_02995"
     CDS             14383..14967
                     /locus_tag="PLS34_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRP86472.1"
                     /translation="MSGRLEIPLFPLQTVLFPGGPLPLRIFEPRYVDMISRCLKTGVG
                     FGVLLIRAGEETGPAATYEIGTVASIVDWDQLSDGTLGVVVLGGARFVLRSARRQDDG
                     LNLGEVELLEPEPTVSLPARFADMARLLEGVFEDLGPHYRHVTPDFEDAGWVGCRLAE
                     LLPIDIEHKQHCLELFDPVARLEYLEPLLKGLGS"
     gene            14967..15314
                     /locus_tag="PLS34_03000"
     CDS             14967..15314
                     /locus_tag="PLS34_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRP86473.1"
                     /translation="MYRAGDGEPLDVAGLVCVHRGTDPIEASLLQGLLESEGIPATVT
                     GADLVGGYSGVPRVCDVRLLVPARRRKDADVVLERYRQAHPADGEWRCAACREVNAAS
                     FESCWSCGREARA"
     gene            complement(15304..>15571)
                     /locus_tag="PLS34_03005"
     CDS             complement(15304..>15571)
                     /locus_tag="PLS34_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRP86474.1"
                     /translation="ADAAGVPFTGHSVGGMFGLFFTGDGPVRSFRQVMACDQKRFKAF
                     FHGMLAEKVYLAPSAFEAGFVSLAHTDEDIEATVAAAARVLARL"
BASE COUNT         2258 a         5329 c         5487 g         2440 t
ORIGIN      
        1 cggttcgcct cgatctggtc acggctgatc tcggcgccca gccgcgccgc ctcgaggctc
       61 aggttgcgct gcagcgcggt gccgacccag tcgtcgacgg acagcggttc cggggagacc
      121 agcggcgtgt ccggtcccgc atcggccagg ccctcgtaat actctccggt gatctcgcgc
      181 aggaactcgc cggccacggc cagcgtgcgc ttggcctcga tcacggcggc ggccgccgag
      241 tcgaagctgg cctgggcctc ctgcacgtcg gtgatcgccg agagcccgac ctcgaagcgc
      301 ttctcggcct gttcgagctg ccggccgatc gcctctttcg ccgcttcggc cgcctgcagc
      361 gtggcgcgcg cgccgagcac gtcgaaatag cgctgtgcga cccgcgccat gaggtcctgc
      421 tcggccgcgc ggaactcggc ctcggcgcgc gccagctgct tgtccgcctg gcgcagcgca
      481 acccactggt cccagcggaa cagggtctgg gtgagccgca gctgccaggc gcgacggggc
      541 tcggtgtcct gctcgaagcc ggaggtcacg gtgatcacct cgccgctgtc gaggcgctgc
      601 ggaaaggtct gcgtgccgga actgtactgg tactcgtagc tgccggtacc ctcgacctgc
      661 ggcagcaggg cggcgcgcgc ctgggggcgt gcctcgagcg cggccaggcg gttggcggcc
      721 gcctcgcgca gggtcgggtc gctgcgcagc gcatcccggt aggcatccaa caggctgacc
      781 gcctgggcgg gagaggctgc cagcatggcc agggcggcga acagcagggg caaacgatgg
      841 cacttcatag gtgaatcctt gaatcttcga tccagaccag tgatatagtt gactatatca
      901 ccatatcgct acgcccgagg caagtggctg ccggcattgc gttctcaata ctgggcaatc
      961 gaggggtcga gctgctcgga ccacgcgagg atgccgcccc ccaggttcca caccttcccg
     1021 aagccctgct gctcgagata gcgggccacg gtcgcgctgc gcccgccgct gcggcacatc
     1081 acgacgatct cgcgctccgg atccagttcg ccgagccggg agggcacttc gcccatcggg
     1141 atatgcaccg cgtcgccgag ttgcgcgatt gccagctccc agtcttcccg cacgtccagc
     1201 aggacaggcg gcggatcggt ccgggcgcgc agcgcgaact ccttcggact catttctctt
     1261 ggcatcagaa cacgaactcc ggggtgtgcg gggcatcgat cagcggcggc aggcaggtct
     1321 cgaatacggt cgcgtcgctc cattcggatt cgccggtgcg caggatgcga cgggcctgca
     1381 ttatcggcgg tcggccggtc accacgaaca ggcgaccgcc cattttgagc aagcggcgca
     1441 gccgatcaag catcccggcg tcgtcgggca gcgagccgtt gacgcagatg acgtcgaaac
     1501 gcccgggaaa ttccatgctc agtccgtcgc cctccagcac cgtcgcattc ccgacgccca
     1561 ccgccgcgag attgtcgcgt gagtgcaccg ccatggcgga atccgattcg gtgctggtca
     1621 cctggccggc gagtgccgcg aggcaggcgg tcaggtagcc cgagccgctg ccgatttcga
     1681 gcgcctcgtc agtgggctgc ggcagcagcg cctgcagcgc gcggccctgc agcttcggcg
     1741 cggccagccg gcggccgggc ccgagcggca ggtcggtgtc ggcataggcc agggcacgca
     1801 actccagcgg caggaactgc tcacgaggca gcgcgcccat cacgtcgagc acgcgcccgt
     1861 cgagcacgtc ccacgcccgg agctgctgtc cgatcatctg gtgtcgtgcg gcgtcgaggt
     1921 tcatccggaa gtgcgctgct acgcgccgcg cggcggcggg aaggctgctc aggttagtgg
     1981 ctcgacgaag gtgtgacaag cgggcgaggc tgcattatgg tgaatgcttg ttcgtgggtc
     2041 acggcccatg ggctaagctg ttgtcttttc gggggacggc gtcgtctcaa ccagtccggg
     2101 ggtctgcatg agtgccaatc cgcgcgacga gctccgcgac cccgcacgcc tcggcgatgc
     2161 ggtcacgcgg ccgttcgccg gctcgacgaa gcttcacgtc gccggcagcc gccccgatat
     2221 ccgcgtagcc atgcgtgaga ttcgacaggc cccgacgccc ggcatccgcg gcgcggagcc
     2281 gaacccgccc atcgtggtct acgacacgtc cggcccctac acggaccctg actaccatgt
     2341 cgacctgctc gccggattgc cggcgctgcg cgcggcctgg gtggaagcgc gcggcgacag
     2401 cgcgccgctg gacgggcaat cttcccggtt tgcgcgggcc cgcgcaggca acccggatgc
     2461 cgccgcgctg gcctttcccg cccggcgcgt ggcacgccgg gcgctggcgg gaagcaacgt
     2521 cacgcagatg cattacgcgc ggcgcggcat cgtcacgccg gagatggaat acgtcgcgct
     2581 gcgcgagaac gccggcctgg aagcccgccg cgaggcatgg gctgccgccg gcctgctcgc
     2641 gcggcacccg ggcgaggctt tcggcgcccg tctgcccgag ctggtgacgc cggagttcgt
     2701 gcgcgccgag gtcgcagccg gacgggccat cattccctgc aacctgaacc accccgagct
     2761 cgagccgatg atcatcggcc gcaatttccg cgtgaaggtg aacgccaata tcggcaattc
     2821 cgcggtgacc tcgtcgatcg ccgaggaagt cgagaaactg gtctgggcga cccgctgggg
     2881 cgcggacacg gtcatggacc tgtccacggg caagcacatc cacgagaccc gcgagtggat
     2941 catccgcaat tccccggtgc cgatcggcac ggtgccgatc taccaggcgc tggagaaggt
     3001 cgacgggcgc gccgaggagc tcaactggga gttgtttcgc gacaccctcg tcgagcaggc
     3061 cgagcagggc gtcgactatt tcaccatcca cgccggcgtc cgcctgccgt tcatcccgct
     3121 gacggcgggg cgcgtcaccg gcatcgtatc gcgcggcggc tcgatcatgg ccaagtggtg
     3181 cctggcgcat caccaggaga atttcctcta cacccgcttc gccgagatct gcgaaatcat
     3241 ggctgcgtac gatgtggcgt tttccctcgg cgacggtctg cgccccggca gcatcgccga
     3301 tgccaacgac cgcgcccagt tcgcggagct cgagacgctc ggcgagctga cgcgcaaggc
     3361 ctgggagcac gaggtgcagg tcatgatcga gggtcccggc cacgtgccga tgcagctgat
     3421 ccgcgagaac atggacaagg agctggcggc ctgcatggag gcgccgttct acacccttgg
     3481 cccgctcacg accgacatcg cgccgggcta cgatcacatc acctcggcga tcggcgcggc
     3541 gcagatcggc tggtacggca ccgcgatgct ctgctacgtg actcccaagg agcacctcgg
     3601 cctgcccgac cgcaaggacg ttcgtgaagg catgatcgcc taccggatcg ccgcgcacgc
     3661 cgccgatctc gccaaggggc atcccggcgc gcagctgcgc gacaatgccc tgtcgaaagc
     3721 gcgcttcgag ttccgctggg aggaccagtt caacctcggg ctggacccgg accgggcgcg
     3781 cgaataccac gaccagacca tgcccaggga ggcgcacaag ctggcgcatt tctgctccat
     3841 gtgcggtccg cagttctgct ccatgaagat cacgcaggaa gtgcgcgact acgcgcggac
     3901 ccacgggctc gacgacccgg ccgcggccat ccgcagcggc atggaggaga aatcgatcga
     3961 gttccgcgcg gatgccgcgc aggtctatcg gcgcgggtga cccgacaggt gagcaagcgg
     4021 acatggcagt gatcgagacc ctcaccatcg gcgtcgcggc cggtgtgctg ggcctgctcg
     4081 gggtaatcat ctggcggcgc aaggcggcgg cgcaccaggg cggtggcgcc gtgccgatgc
     4141 gcatgagcga ggtccgggag cgcgccgcgc cacgggcgga cgcacgcgcc gcgccgtacg
     4201 cccacgagca gtcccagccg ctgtcggtgc tcgccgacca gcccatgttc ctgcgcctgg
     4261 ccgagcgggt cgatgacgcc gtcctgctgc acgacgacga gatccattac gccaacgcgg
     4321 ccgctgaagc gctcttggcc gccggcgagc cgctggccgg ccggcgcctc gtcgacctgg
     4381 tccagccgga gcacctggaa gggctgcagg cctggctgaa ggcgcgccgc gcggggcggc
     4441 agtcggcggt gcggctgcgg ctgcgcgacg gcaacgactt gcccatcgaa tgcgaactgg
     4501 cgggcttccc gctccctgca ggcagccgca tcgtcggcac ggtggtgcgc gccacgcgcg
     4561 acgagctgct aagttctgca cggcagcagg gcacgcgcgt tgccgaggcg acgctggaat
     4621 ccatcagcga aggcgtcatc accaccgaca tgcgcgggca cgtcgattac ctcaactcgg
     4681 ctgcggaggc gctgctgggc tgccagcgcg acgccgtgca cggccggccc ttcggcgagg
     4741 tggtcacgct ggtcgacgag ggcgaccggc ggccgctcgg cgacccggtg atgcgctgcc
     4801 tcgaggagcg ccgcagggtg gacctcgggc ggcgggcgct cttgatatcg cgcgcctcgg
     4861 gcaacgaatt ctcggtcgag ttgcgcgcct cgccgatccg cgccggcggc caggagctca
     4921 gcggctgcgt ggtggtgctg cacgacgtat ccgagattcg gggcctgacg cgccagatgt
     4981 cctaccaggc cagccacgac ccgctcaccg gcctggtcaa ccggcgcgaa ttcgagcgac
     5041 gcctcgagga gggcctgaag accgcgcgtt cgggcaacgg cggccacgta ctctgctacc
     5101 tggacctcga tcgcttcaag gcggtcaacg acaccagcgg ccacgtggcg ggcgacaaca
     5161 tgctgcgcga gatcgctggc gtgctcaagg acaagatccg cgattcggac gtcgtggcga
     5221 gagtcggggg cgacgagttc ggcatgctgc tgatcggctg cccgctcgag aaggcgcgcc
     5281 agattgccga cgacgtatgc gcggcgatcc gcgattaccg cttcgtctgg cgggacaaga
     5341 tcttcaatgt gggcgtcagc gtcggcctgg tgcagatcgg gccggagagc ggctcgatcg
     5401 aggacacgct gagcgcggcg gactccgcct gctacatcgc caaacagcag ggccgcggcc
     5461 gcattcacgt ctattcctcg cgcgacgagg ccgcggcgcg ccagcgcggt gaaatcgtct
     5521 ggctgcagcg gctgcaggcg gcgctcaagg aaaatcgctt cgagctgtac acccagccga
     5581 tcgtggcact ggcgggccgg ccggacaccg gcccggcctt cgaagtgttg ctgcggctga
     5641 aggacgcgga cggcgagagc cagccgatgg atttcatgca ggccgccgag cgctaccacc
     5701 tcatgccgag cgtggaccgc tgggtggtgc aggccacgtt cgcggccatc gccgccggtg
     5761 cgctcaagct gccctcggga cgcagcgccg ccatcaacat ttccagccag acgctcgccg
     5821 acccgcagtt cctcgagttc gtggtcgagt gcctggaccg tagcggcgtg cgcccggagc
     5881 aggtgtgctt cgagctgtcc gagtccgtcg tggccgccag catgagccac acggcccggt
     5941 tcatcgccgt gctccacgga ctgggctgcc gcttcacgct cgacgatttc ggcagcggtc
     6001 tcgggtcctt cgccaacctg aagcagctcg ccctggatta cctgaagatc gacggctcct
     6061 tcatccgcgg actgggcaac gacgaggtcg acgtggccat ggtgaccgcg atgatcgagc
     6121 tggcgcggac cctcggcatc aaggtgatcg ccgagcacgt cgagaccgag cgggcgctcg
     6181 aactggtcag ggcgctcaag gtcgatttcg tccagggcta ctcggtgggc cgcccgcagc
     6241 cgctcggatc cgcgcgggcc tgagccccgc cgcctaccaa gccgccgccg agccgctata
     6301 atgcgcggct ggcaattgcc acgcgggccg gttcggccgg cgcaatcgaa gttggggggc
     6361 ggtgccggct gcgtcagccg gccccggaaa cgcaggatcg gatgagactg gagagacgct
     6421 gtcatgctga ttgaatacgg actggtcgcg gccctgggct gcgccatcgt ggcgatactc
     6481 tacggcgcct ggtcggtacg ctgggtgctc gcccagtcgg ccggcacgcc gcggatgcag
     6541 gagattgcgg gagccatcca ggagggcgcg caggcctacc tgaaccgcca gtacacgacg
     6601 atcggcatcg tcggggtgat cctgttcctc gtggtgggac tggccctcga ctggctgacc
     6661 gcgggcggct tcgccatcgg cgcgatcctg tcggcggcga ccggctacat cggcatgttc
     6721 gtctcggtgc gcgccaacgt gcgcactgcg gaggccgcgc gccgcgggct gtcgccggcc
     6781 ctcgatgtcg catttcgcgg cggcgccgtc accggcatcc tggtggtcgg gctcggcatg
     6841 ctgggcacgg cgggctatta ctggatcctg ctggccaccg gacacgagga ccacgcggtg
     6901 cacgcgttga tcgggctggc cttcggctcg tcgctgatct cgattttcgc gcgcctgggc
     6961 ggcggcatct tcaccaaggg cgccgacgtc ggcgcggacc tggtcggcaa ggtcgaggcg
     7021 ggcatcccgg aagacgatcc ccgcaatccc gccgtgatcg cggacaacgt gggcgacaac
     7081 gtgggcgatt gcgccggcat ggcggccgac ctgttcgaga cctacgcggt gaccatcatc
     7141 gcgaccatgc tgctggcggt ggtgatggcg accgaggtcg gcccgaacgg cgtgctgtac
     7201 ccgctggtcc tgggtggcgc ttccatcctg gcgtcggtgg tgggcacgtt cttcgtcaag
     7261 atcagcggca gcggcaagat catgaacgcc ctgtacaagg gcgtcatcgt gtccggcgtg
     7321 ctggccgcgg tggccttcta ccccgtgacc accagcatga tgggtgattc gccgctcggc
     7381 gcgaccaacc tgttcctctg cggcgtggtg ggcctggtgc tgaccgccgc aatggtggtg
     7441 atcaccgagt actacacggc gacggaatac agcccggtgc gctcggtggc ggcggcctcc
     7501 gagaccggtc atgcgaccaa catcatcgct ggcctggcgg tctcgatgaa gtccaccgcc
     7561 ttgccggtgc tggcggtctg catcgccatc tggaccacgc attcgctggg cggcctgtac
     7621 ggcatcgcca tcgcggcgac ggccatgctg tcgatggccg gcatggtggt cgcgcttgac
     7681 gcctacggcc cgatcaccga caacgccggc ggcatcgcgg agatgtcgga cctgccgagc
     7741 gaggtgcgcg acatcaccga cccgctggac gcggtcggca acaccaccaa ggcggtcacc
     7801 aagggctacg ccatcggctc ggccggcctg gcggcgctgg tgctgttcgc ggactacacc
     7861 cacggcctcg aagcggccgg caaggcgacc gagttcctgc tcgacagtcc gatgaccatc
     7921 atcggcctgt tcatcggcgg actgatcccg ttcctgttcg ccgccatggc gatggaggcg
     7981 gtcgggcgcg ccgcgggttc ggtggtgaac gaggtgcggc gccagttccg cgagattccc
     8041 ggcatcatgg agagcacggc acggcccgat tactcgcgcg ccgtggacat gctgaccaag
     8101 gcggccatca aggaaatgct cgtgccgtcg ctgctgccgg ttgcggcgcc gatcgtggtc
     8161 ggtttcctgc tcggtccgga ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn ncgcctggga caacgccaag aagtacatcg aggacggcaa
     8281 cttcggcggc aaggggtccg aggcccacaa ggcggcagtc accggcgata cggtcggcga
     8341 cccctacaag gacaccgcgg gcccggcgat caacccgttg atcaagatca tcaacatcgt
     8401 cgcgctgttg ctggtgccgc tgctctagat atcagtggcg cgcgcggcgc cgccaggggc
     8461 cgcgccaccg gacgcaaacg gacgacgggc caggggaaac cctggcccgt ttttttcctg
     8521 tcctcagccg gtccagtccg gcagtttctt cttcgccagc ttgttcttga gcttgacgta
     8581 gaccggcagg cccgcacggt agggcggata gtcctcaccc ttgatcagcg gttcgagata
     8641 gcgccggcag gccttggtga tgtggaagcc gtcctcggtg atgtacttct tcggcagctt
     8701 cttttcgacg ttggcaacgg ccgagaggtc cgcctcgccc actttccagc gatagggctg
     8761 atccgagctg cgcacgatga ccggcagctt gccgtgcacg cccgcgagcg ccattttcac
     8821 cgccgccttg ccgaccgcgt aggcctgttc cacgtcgacc ttcgacgcga tgtgccgggc
     8881 ggagcgctgc aggtagtcgg ccaccgccca gtggaacttg tagcccagcc ggccgcgcac
     8941 gatctcggcc aggtgcgggg cgacgccgcc gagctgggcg tggccaaagg cgtcccgggt
     9001 gccggactcg gccacaaagc tgccgtcggg cctgcggatg ccctccgagg ccacgatcac
     9061 gcagctgtcg tgctgctcga cgctcttgcg caccatttcg acgaagcgct cctcgtcgaa
     9121 gggcacctcc gggaacagga tgatgtgcgg cggctcgctg cggtccttgc cggccaggcc
     9181 gccggccgcg gcgatccagc cggcatgccg gcccatgacc tccaggatga agaccttggt
     9241 ctgcggcatg gagccgacgt cgatgcccgc ctcgagggtc gaaacggcca cgtacttggc
     9301 gaccgagccg aagccggggc agttgtccgt gaccggcagg tcgttgtcga cggtcttggg
     9361 aacgccgacg caggtgatct cgtagtccat ctgcctggcg acctgcgcga ccttgttggc
     9421 cgtatccatg gagtcgccgc cgccgttgta gatgaagtag ccgatgtcat gcgcgcggaa
     9481 cacctcgatg aggcgctggt actcggcggc gctctgctcg atgctcttga gcttgtagcg
     9541 caccgagccg aaggcgccgc ccggtgtgtg ccgcagggcc gcgatgtctt tcgccgactc
     9601 cttgcccgtg tcgatgagtt cctcccgcag cgcgccgacg atgcccatgc gaccggcgta
     9661 gaccttgccg atacggtccc gcctggcgcg cgcggtctcg atgaccccgc aggcggtggc
     9721 gttgatcacg gcggtcacgc cgccggactg ggcgtagaac aggttgcgct tcttcttcat
     9781 tctgcctccg tggtggggcc tgcttgcagg ccggcgagga gcccggtgcg gcgcttcatc
     9841 atcgcgccgg ggcggcgtcg agagcatagc ggattaccgt ggggccggtc atgccgggcc
     9901 cgtgaaggtt tgaccgcgcg cgggcggggc ggcaagcttg ggcgcccggc ctggacggcc
     9961 gtgcgacagc agtgggagaa gattgcatgc gcattgtatt gctcggcgcc cccggctccg
    10021 ggaaagggac ccaggcgaag aagctggtgg aggatttcgg ctacccgcag gtgtccaccg
    10081 gcgacctgct ccgggacgcc gtggcccgca gcaccgcgca cgggctcaag gcgaaggcgg
    10141 cgatggacgc cggcaagctg gtgtccgatg acatcgtgct gggaattctc agggagcgtg
    10201 tggcccagcc ggacgccgcc cgcggcttca tcctggatgg ctttccgcgc aatctcgccc
    10261 aggccgaggc gctcgaggag gtgctggccg agctcgggca gccgctcgac gccgcagtcc
    10321 tcatggacct ggatcatgcc attctgctca agcggctcac cggacggcgc agctgcaccg
    10381 gctgcggccg cgtgtacaac atctacttcc tgccgccgcc cgccgacggg cgctgcggca
    10441 gttgcggcgg cgaactcgtg cagcgctcgg acgtcaagga ggagaccatc ggtcgccgcc
    10501 tcgaagtcta tcgggcggag accgagcccc tggtggacta ctaccggcag ctcggcaagc
    10561 tcaggaccgt cgatgccgat gcgcccttcg acgaggtcta cgcacgcctc aaggacgcac
    10621 tcggcctgta acaaactgcg gcggccgcgc gtcctgctca gtccgtcgcg agcatccgca
    10681 gcagctcttc gccggccagc tcgcgccgcc agccgtgcag cagcggcagg tcgcgctgac
    10741 cctgcaccag cgcctcgatg tccctgcgcg tggcgagcag cggcgccgag atccgctggt
    10801 tacgggcgat ttccctgagg cggtccatca gccggcgggc ccgtcgctcc tcggccggtt
    10861 cgaggcggcc ggcggcggga cgctccggcg ggggcgcggt atcggcgtcc ctggcgatcc
    10921 gcaacagggt ctcgccgtgc ctgcgcaaca gcgctggcgg caggccctcg acctgctcca
    10981 gttcggcggg gtcccgtggc gcgcgcgtcg ccagcgccac gatcgcctcg tccctgatga
    11041 tccagcgccg cgggcggtcg gcccggcgcg cctccagctc ccgccattcg gccagcgccg
    11101 ccgctcgcgc gagcgcggcc ccctccagtt gcccggcgcc cttgaccctg cgccacgccc
    11161 cggccgcgtc gaaagcgtac agggcagggt cgcacaggac cgagcattcg gcgtccgccc
    11221 actcgccgcg gccgagctgc tccagcgcct ggtccagcct tgctgccagc ggcagcagcc
    11281 agcgaacgtc cgcggcggca taccgcaact gctcgtcgga cagcggccgg cggccccagt
    11341 cggtacgcgc atggtccttc ggcaaggtca cgccgagctc ggcgccggtc agctgcgtgt
    11401 agcccacctg gtccggatgg ccgagcatcg ccgcggcgat ctgcgtatcc cagatcgggg
    11461 cgggcacctg gccgaggtgc tcgttgagca cctccaggtc ctggcgcgcc gaatggaaaa
    11521 ccttgcgcac ggtggggtcg agcagcaggt cggcgaacgg cccggggccg tcgagcgcca
    11581 gcatgtcgag gcaggcgacg cgctcgccgt cggacacctg catcaggcac agcctggccc
    11641 ggtaggtgct ctcgcgcacg aactcggtgt cgacggcgag ttcccggacg cctgcccact
    11701 cgcgggccag cgacgccgca tcggcggcct tgtcgatgaa ttcgtaatca ctcatggcgg
    11761 cattatccca gaacggcagg tcggggtccg gcgcttgcgc ggcgcggccg ggcgccgggt
    11821 atgatgccgg ctggcaaagg tgacaccatg caggccctga tccgcttgtt catcgaagtc
    11881 gcactgcatc ggaggggacc gcaggacgtg cccacctccg gcgccgtgtt cggactggcg
    11941 ctgttcgctt acctggcgat cggcgccgcc gtgctgtggc cgtccgccgc cggcaccgag
    12001 gcgctgctgg gccagctggc cctcgacgtt gcgctgctgc tgctggtgtt cggcgggctg
    12061 cttgcgggta ccgggcgagc gggccggctg cggcagaccc tgaccgcgct gctcggcacg
    12121 ggcgcgctgc tcagtgccgt cgcgctgccg ttcgtgtggc tggcggcgcg ggcgctgggc
    12181 aacggcgccc cggtcccggg catggaactg ccggcgctgg tgagcaccac ggcgctgttc
    12241 atgctgctgc tggcctcctt gctggtgacc gggcacatcc tgcgccacgc catggactgg
    12301 agctacgccg cgggcgtgct cacagccgtg ctgtatttcg ccctgagcgt ggcgatgttc
    12361 cgccgctact tcccggtcgc gtgatgcacc tccatatcct cggcattggc ggcaccttca
    12421 tggcgggcat cgcggcgctg gcgcgcgcgc agggtcatcg tgtcaccggc tcggaccgcg
    12481 cgctgtaccc accgatgagc acgcagctcg ccgcgctgga gatcgagctc atcgagggct
    12541 acgacaacga ccggctggac ccggcgccgg actgcgtcgt ggtcggcaac gtgctgcgcc
    12601 gcggcctgcc cgtggtagag cggctgctgg actcgggcct gccgtactgc tccgggcccg
    12661 agtggctggc gcgcgaggtg ctgcgcgatc gctggacgct ggccgtggcc ggcacccacg
    12721 gcaagacgac caccaccagc atcctggcct tcctgctgga atccgccggc ctgcggcccg
    12781 gcttcctggt cggcggcatc ccggcggact tcggcgtgtc ggcgcggctg ggcgaggcgc
    12841 cctttttcgt cgtcgaggcc gacgagtacg acacggcctt tttcgacaag cgctccaagt
    12901 tcgtccatta ccgcccgcgc acgctgatcc tgggcaacct ggaattcgat cacgcggaca
    12961 tcttcccgga cctcgccgcc atccagcgcc agtttcatca ccttgtgcgg acggtgcccg
    13021 gcaccggctt gatcgtgtcc aaggcgggtg acgcggcgct ggaagaagtg ctggcgcagg
    13081 gctgctggac gccggtcgag cgcttccgcg ggcccgggga ggccacgccg gacgccggcc
    13141 atgcggcacg actcgtgccg ggcggcttcg aagtcctcgc gccggacggt gcgtcgctgg
    13201 cgacggtgca ctgggccatg ccgggcgcac acaatgccga gaacgcgctg gcggcgatgc
    13261 tcgcggcgcg ccacgccggc gtgccaatgg aagtctcggc gcgggcgctg gcaaattttc
    13321 ggggcgtgaa gcgccggcag gagtggatcg gaacggtcga cggcgtcgaa ctgttcgatg
    13381 atttcgcgca ccacccgacg gccatcgcgc gcacgctgga ggcgatgcgc gacggcctgt
    13441 cggccgggcg actggtggcg gtgctggagc cgcgctccaa caccatgcgc atgggcgtcc
    13501 atgcgggcag cctcgcccgg tcgctggcgg gtgctgaccg cgtgttcgtg catgcgcccg
    13561 ccgacctcgg ctgggacgcg gccgctgcct tggcggcgct gggcgacgag gcgcgcgtgt
    13621 gcgccgaact ggcggagctg gagactatac tgctggagga attgcgcgcc ggcgaccgcg
    13681 tggtgctgat gtccaacggc agcttcggcg gcctgcatgg gcgtttgcgt gacgggctcg
    13741 aggcgcgcgc gaggagagcg accgaatgag catgcacgtg gcgctggcga tgacgggagc
    13801 ctcgggggcg cagtacgggc tgcgcctgct tgagtgcctg ttgggcgcag ggtgccgggt
    13861 cacgctgatg atctcgaggc ccgcccaggt cgtcatcggc atggagaccg acctcgacct
    13921 gcccgcgcgc cccgccgaga ttcgccgctt cttctgtgcc cgccacggcg tgggggaatc
    13981 acggctggcg gtgcacggcc gtgaggaatg gactgcgccg gtggccagcg gctcagccgc
    14041 gcccgacgcc atggtggtgt gtccctgtac gacggccacc gtctcggcgc tggcgaccgg
    14101 cgccagccgc agcctgatcg aacgcgccgg cgacgtcgtc ctgaaagagc gccgccggct
    14161 ggtactggtg gtgcgcgaga cgccgttctc gccgatccac ctggagaaca tgctgacgct
    14221 ggcgcgcgcc ggcgcggtga tcctgcctgc caacccgggc ttctaccatc gtccggcaca
    14281 ggtcgcggac ctggtcgact tcgtggtggc gcgcgtgctc gaccacctgg ggatcgccca
    14341 gtcgctcgtg gccccctggg gcggaacgca ggacgcggac gaatgagcgg ccggctggag
    14401 attccgctgt ttcccctcca gacggtgcta ttcccgggcg gaccgctgcc gctgaggatt
    14461 ttcgagccgc gctacgtcga catgatcagc cgctgcctca agacgggcgt cggcttcggc
    14521 gtgctgctga tccgcgccgg cgaggagacc gggccggccg ccacgtacga gatcgggacc
    14581 gtggccagca tcgtggactg ggaccagctg agcgacggca cgctcggcgt ggtggtgctc
    14641 ggcggagcgc gcttcgtgct gcgctccgcc cggcggcagg atgacggcct gaacctcggc
    14701 gaggtcgagt tgctggagcc ggagccgacg gtatcgctgc ccgcccgctt tgccgacatg
    14761 gcgcggctgc tggaaggcgt gttcgaggac ctcggaccgc attaccgtca cgtgacgccc
    14821 gacttcgagg acgcgggctg ggtgggctgc cggctggccg agctgctgcc gatcgacatc
    14881 gagcacaagc agcattgcct ggaactgttc gacccggtcg cgcggctcga atatctcgag
    14941 ccgctgctga aggggctcgg gagctgatgt accgcgcggg cgacggcgag ccgctggacg
    15001 tcgccggcct ggtttgcgtc catcgcggca ccgacccgat cgaggccagc ctgctgcagg
    15061 gcctgctgga gagcgagggc attcccgcca cggtcaccgg cgcggatctc gtcggcggtt
    15121 acagcggcgt gcccagggtc tgcgacgtgc gcctgttggt gcccgcgcgc cggcgtaagg
    15181 acgccgatgt ggtgctcgag cgctaccggc aggcacaccc tgccgacggt gaatggcgct
    15241 gcgccgcttg ccgcgaggtc aacgccgcga gcttcgagtc ctgctggtcc tgcgggcgcg
    15301 aggctagagc ctagccagca cgcgcgcggc cgcagccacg gtcgcctcga tatcctcgtc
    15361 cgtgtgcgcc agcgacacga agccggcctc gaaggccgac ggcgcaaggt acaccttttc
    15421 cgccagcatg ccgtggaaga aggccttgaa ccgcttctgg tcgcaggcca tgacctgcct
    15481 gaagctgcgc accggcccgt cgccggtgaa aaacaggccg aacatgccgc caaccgagtg
    15541 gcccgtgaac ggcacgcccg ccgcatccgc g
//
LOCUS       DAOMPG010000033        15410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1670_length_15410_cov_4.602475, whole genome shotgun sequence.
ACCESSION   DAOMPG010000033 DAOMPG010000000
VERSION     DAOMPG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_15410_cov_4.602475"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLS34_03010"
     CDS             complement(<1..703)
                     /locus_tag="PLS34_03010"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRP86475.1"
                     /translation="MSEEQIESNRRLYGISRWSDGYFDIGRRGRLVVRPFRNGAEIDL
                     VELVRRARLAGLAPPILFRFTDILRDRLDRLHNAFRKAMAAAEYESRYRVVYPVKVNQ
                     QRSVVDTLVSHGGARVGLETGSKPELMAVLGVSPPGGLIVCNGYKDHEYLELALIAQR
                     LGHEVYIVVEKLSELDRIIQLAREFELEPRLGVRLRLSSVGAGNWQDTGGEKSKFGLT
                     AGQLLQAAERATQAGM"
     gene            complement(867..1310)
                     /locus_tag="PLS34_03015"
     CDS             complement(867..1310)
                     /locus_tag="PLS34_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRP86476.1"
                     /translation="MRFLLPALLAAMLATPALANEVYRTVDERGVVHYSDRPLSDRSQ
                     RVKVESKATDPARAAEESRALLEGDPQREQRGTERQAVAAAQAEQAQIRAEACRQARA
                     AVETYERSPRLYETLADGGRRYLSDEEMIAARAQARQAVLDYCDD"
     gene            complement(1325..1663)
                     /gene="glnK"
                     /locus_tag="PLS34_03020"
     CDS             complement(1325..1663)
                     /gene="glnK"
                     /locus_tag="PLS34_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRP86477.1"
                     /translation="MKMVCAIIKPFKLDDVREALADLGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLELAVPDDSLERVLESIVNTARTGKIGDGKIFVYDLEQAIRI
                     RTGETDEKAL"
     gene            complement(1867..3120)
                     /locus_tag="PLS34_03025"
     CDS             complement(1867..3120)
                     /locus_tag="PLS34_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRP86478.1"
                     /translation="MEQVMQLSYALDTFYFLMSGALVMWMAAGFTMLEAGLVRAKNTA
                     EILTKNVGLYSIACLMYMLCGYGIMYGDGSGIIPGFGMLGSADNAVAEVVAGGDDAPY
                     YSNLSDFFFQVVFVATAMSIVSGAVAERMKLWSFFAFAVVMTAVIYPVQGYWTWGGGF
                     LGETFGYSDYAGSGIVHMTGAAAALAGVLVLGARKGKYGAKGQVNAIPGSNLPLATLG
                     TFILWMGWFGFNGGSELKVSDVESANNVALVFTNTNLAAAAGLVAALLLARAWFGKAD
                     LTMALNGALAGLVSITADPLSPTPLLAMIIGAVGGLLVVSSIVLLDRKFRIDDPVGAI
                     SVHGTAGIWGVLAVLLSNGDATVSGQLIGLAVIFAWVFFASLLVWFVLKKTVGVRVTE
                     QQEYEGVDLHECGLEAYPEFTKAGD"
     gene            complement(3137..3475)
                     /locus_tag="PLS34_03030"
     CDS             complement(3137..3475)
                     /locus_tag="PLS34_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRP86479.1"
                     /translation="MKLVTAIIKPFKLDDVRQALADVGVKGVTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFLPKMKVEVAIDDDLLEQVVEVISNTARTGKIGDGKIFIMPLEQVIRI
                     RTGETGPEAV"
     gene            complement(3481..4107)
                     /locus_tag="PLS34_03035"
     CDS             complement(3481..4107)
                     /locus_tag="PLS34_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRP86480.1"
                     /translation="MGRSRYLLVALALGTAQPALASDFSANIGMMSDYVYRGIKQNDA
                     AAFAGLDYENSGFYVGTWAAEVGEGLEYDLYAGYGGSIGDFSYGIGYTGYFYTDDFDD
                     TYHELNLTAGYGLFSLEYSLGRYDNFSGPTLDYGFIAGTVEYQGFYATYGSFSKDFEG
                     DYVEVGYGAEVSGFDLGVGLVVSDSDLSGTGSSESTLMFSIGKTFDLR"
     gene            4296..4559
                     /locus_tag="PLS34_03040"
     CDS             4296..4559
                     /locus_tag="PLS34_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRP86481.1"
                     /translation="MMDTKTLNIDELARRLADALPSGLKALQADAEQNFRAVLQAGLA
                     RLDLVTREEFDVQAAVLKRTREKLAALETRLAALEQAPERTAD"
     gene            4657..6153
                     /locus_tag="PLS34_03045"
     CDS             4657..6153
                     /locus_tag="PLS34_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRP86482.1"
                     /translation="MAMATVLSRAQCGLDALPVSVEAYVGGGLPGFSLVGLPATAVRE
                     SRDRVRAALLTCGFDFPDGRVIVNLAPADLPKEGGRFDLPIALGVLAASGQVPRNGLK
                     VTEFIGELSLNGDLRPVHAALCAAIATARDGRGLVVPAASAAEAAAAGEGEVRAAASL
                     LAVAAHLQGRQPLPVVAAPPRVMAGIHRGPDLSEVRGQLRGRRLLELAAAGGHNLLFV
                     GPPGTGKTMLARRLPGLLPPLDARTALRVAAIASAAGKPFRPDDWGRRPFRAPHHSAS
                     SVALTGGGSIPRPGEVTLAHHGVLFLDELPEFRRDALESLRQPLEDGELTISRAALQR
                     TFPARFMLVAAMNPCPCGHLGDPRGNCRCSAEAVRRYRARISGPLLDRVDLVVELPRP
                     SADELLGPPGEESAEVRKRVLAVRQASLKRQGCVNARLEGKALDRHAPLGPAQRALLA
                     RSADRLGLSARAQTRVRRVARTIADAAGADAIEEKHLAEALACRGMPE"
     gene            complement(6164..6643)
                     /locus_tag="PLS34_03050"
     CDS             complement(6164..6643)
                     /locus_tag="PLS34_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRP86483.1"
                     /translation="MTVLLWILAVLLVVAGLAGLVLPALPGPPLLFLGLLLAAWAENF
                     VHVGAGTLTALGVMAVLAFLADLVAGAFGARRYGASPRSVVGATLGAVVGIFFGLPGL
                     LLGPFVGAVLGELSAQRDLASASRAGWGATVGLAIGIAAKLALGIAMVGLFLVVRFV"
     gene            6761..8758
                     /locus_tag="PLS34_03055"
     CDS             6761..8758
                     /locus_tag="PLS34_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP86484.1"
                     /translation="MSLNPAQLEAVRYTAGPLLVLAGAGSGKTRVITEKIAALVAGGM
                     PAYSIAAVTFTNKAAREMRQRAGALLDAETAKQLRISTFHTLGLNILRREYGNLGYKR
                     GFTIFDANDSRNLVKELLKNDNADTEGAIEAACAAISAWKSELVAPEAALAAATDELE
                     QARARQYAAYQRALQAYNALDFDDLIARPVFLFRELPAVLAAWQDRINYLLGDEYQDT
                     NFAQYQLMGQLVGARGAFTVVGDDDQSIYAWRGARPDNLKSLAADYPALKVIKLEQNY
                     RSSGRILAAANGLIANNPHLFDKQLWSAHGPGDRIRVLVARDGADEADRVAMEIMRMR
                     MERGGEWRDFAVLYRGNHQSRVFEQALRALEIPYSVTGGISFFDRAEVKDVLAYLRLI
                     ANPDDDAAFLRIVNVPRREIGAATLEKLGAWASSRGLGLLAASLEAGLHAVLPARTVE
                     RLHDFARRMIELGDEAEDGARVDELARELIEELGYRDWLFENARDPRQAETANENLAE
                     LLDWLGRASRSGDEPTLADALRRLSLMDMLDRQSEDETDDRVRLMTLHAAKGLEFPTV
                     FLVGCEEELLPHRANLVGPGLEEERRLAYVGITRARQRLFLTYARRRKRFGETLECEP
                     SRFLEELPAEALEWEGRVELSAEETKARGQSAIAGLRSLLE"
     gene            complement(8858..9400)
                     /locus_tag="PLS34_03060"
     CDS             complement(8858..9400)
                     /locus_tag="PLS34_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP86485.1"
                     /translation="MSAEDDRKFFDTFMLVLGILVVVTIAIYFLATAMSNRTQAVHIK
                     EDPMYQQRVAERIRPVGQVAVAGRDNSALRDPGGVAPATMEPPAAAPQAAADLDGEAV
                     YKAACTACHGAGIAGAPKTGDADAWAGRVDQGMDTLVRHAIEGFQGQAGYMPPRGGNS
                     SLTDQQVRDAVQYMVDQLGG"
     gene            complement(9512..9937)
                     /locus_tag="PLS34_03065"
     CDS             complement(9512..9937)
                     /locus_tag="PLS34_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRP86486.1"
                     /translation="MTLIQLIYVSTARQEYDTTELDRILASSVRHNAAHSVTGMLLYL
                     NGSFMQVLEGAEAAVDETYGRIERDPRHTDLFLLERTPIEARSFAGWHMGFRRLGEVE
                     AQALPGFAPFLSHGFDAASIGARPGLALDILRGFASAGR"
     gene            10014..10090
                     /locus_tag="PLS34_03070"
     tRNA            10014..10090
                     /locus_tag="PLS34_03070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10048..10050,aa:Arg,seq:ccg)
     gene            complement(10378..10701)
                     /locus_tag="PLS34_03075"
     CDS             complement(10378..10701)
                     /locus_tag="PLS34_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006012642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HRP86487.1"
                     /translation="MKRDFSKSRRSILRGSALGLAALAGSALLPVSRLQAADSRVSED
                     DPTAKSLKYVHDAEKSARPSADQFCYNCRYFKGTQADAWAPCDLFPGKQVAGPGWCNV
                     WAKKG"
     gene            complement(10771..12216)
                     /locus_tag="PLS34_03080"
     CDS             complement(10771..12216)
                     /locus_tag="PLS34_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86488.1"
                     /translation="MRFLNKGLLGPAGILFIALFAANEAYAVAGFTRQTGMNCNSCHT
                     LHGAPTPNFTFTGKKFNAVGYRLPGKPHHTQERAMEQGTPDDLGEFLTMLPTTFSGRM
                     QYTPYQKTKGATATDWGEATSNPTSRLAFFPFLGPIGDHFGVWTEFYIVPFTSEDNEW
                     GIADTSYEEFDFRYIINPENMDYTIGLAFNNQSIYELFGFGPYPGLPSYLNRGVIGGY
                     THPNKGTLTAYGWMYDRWIWALGMNTGDTNMGWDENNLLGFFGYALRNRNDNELWINI
                     AARTGSDVMPLVTANGAQASSRSFQYRDAVGGVSNTRRPPGDPCPGVAGRRPAGSCAY
                     LAEEIDSATSVDVEVRWGGQNVGSFFNSQGTTGPWSFETVFRVGFNSEDYIDGAKTKR
                     NTWGVQTVIGYNHTYFIKPSIQDSFTYEFTDLAGTKYDIDTKISWNLTLAYKPVENFL
                     IYLAYSNNQSNSLTGSANTGRVINVTADISF"
     gene            complement(12281..12643)
                     /locus_tag="PLS34_03085"
     CDS             complement(12281..12643)
                     /locus_tag="PLS34_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP86489.1"
                     /translation="MKAIGLGVVALGSLLALGAVGISASKASAEEKFDGKWRYYANCA
                     ACHGQDGSGIYAFGPALKGNAFVQNAPAQIIIEVIQKGRNYGNRTHTAYMGMPAFHFI
                     RGGEAEALVAYLKGDLQE"
     gene            12901..13410
                     /locus_tag="PLS34_03090"
     CDS             12901..13410
                     /locus_tag="PLS34_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRP86490.1"
                     /translation="MDERERYWRRVKGMLMLQTGVPQAEVARRLGVTRAAVSGWKGRL
                     AGGETPHTLPKHRGRVPRLSHEDCRVLRELLQRESEGGRRLKLEEIGRLIAAHFSIEF
                     SVAQVSRIAHGIGWSVQWGRLVPPRSERFRAGFRRAQQARLARLATLMNNIAVVHDGD
                     EQLGRQDPQ"
     gene            13493..14173
                     /locus_tag="PLS34_03095"
     CDS             13493..14173
                     /locus_tag="PLS34_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86491.1"
                     /translation="MLLGIVLLLAFVHPPAQADSEALGDPLHRYDEHTPSANTMQRAR
                     RAYRANRHRAAFEYYKVAAFWADKLAQYNVGVMYLQGQGVEFDPVRGWAWLELSAERK
                     YPDMVAAADQLSALLDGAQRQAARRILEEELLPEYGDDVAIERTAKEMRRELRRGTGS
                     RLGSRASLSAVTIIDGNGMTRRGDEFYDPAKWDFENIVRYETRLMFQLARGRVEAGEL
                     VIVEDEDQ"
     gene            14179..15180
                     /locus_tag="PLS34_03100"
     CDS             14179..15180
                     /locus_tag="PLS34_03100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRP86492.1"
                     /translation="MRRRAAILVACGLLLVFIVLSMTRDEEYGRDWLSWLPGQSRHVF
                     AHVPCGIPVHYALAAVDPGFGLDRDTVMAALVEAVSLWQPPSGPLLFLESDHALAIQV
                     SLRYDERQQAANTRSSLRHGLERDRGAFEDDEAMLLQWGERIDAARASHERAAAALAA
                     RARAYEQDVTAWNAGRGERTETRRQALETESALLRDALADLERAGLALNADVEAYNRR
                     AADLRQRADDFQARVARYNAASSASPVESGRYSYAPGEGRRIEVYRAESFDELVLVLA
                     HELGHALGIGHVDEPGAVMHAMLHDGGLLQSDPTRPLELSAADKAALVAVCGQRLRP"
BASE COUNT         2420 a         5148 c         5383 g         2459 t
ORIGIN      
        1 ccatgccggc ctgcgtggca cgctcggcgg cctgcagcag ctggccggcc gtgaggccga
       61 acttggattt ctcgccgccg gtgtcctgcc agttgccggc gccgacgctc gacagccgca
      121 ggcgcacgcc gaggcgcggc tcgagctcga actcgcgcgc cagctggatg atgcggtcga
      181 gctcggagag cttctccacc acgatgtaca cctcgtggcc gaggcgctgc gcgatcagcg
      241 ccagctcgag gtactcgtgg tccttgtagc cgttgcagac gatcagcccg cccggcggcg
      301 agacgccgag caccgccatc agctccggct tgctgcccgt ctcgaggccg acgcgcgcgc
      361 caccgtggct gaccagcgtg tccaccacgc tgcgctgctg gttgaccttg acgggataga
      421 ccacgcggta gcggctctcg tactcggccg cagccatggc cttgcggaag gcgttgtgca
      481 gccggtcgag gcggtcccgc aggatgtccg tgaagcggaa caggatgggc ggcgcgagcc
      541 ccgccagccg cgcgcggcgc accagctcga ccaggtcgat ctcggcaccg ttacggaacg
      601 ggcgcaccac cagccggccg cgcctgccga tgtcgaagta gccgtcgctc cagcgggaga
      661 tcccgtacag gcggcgattg gactcgatct gttcctcgct catccgcgga cgcctccagg
      721 gggtgccgag ccggggaaaa agggcgcgca gtatagcgca atcgtgcgcc cggcaagggc
      781 gtcgacctca ggcatgggca ggaagcgccg ggcgcgggcg cggcggtcgg gacggggcgc
      841 cgcaagggct gcgggcggcg gcgggcctag tcgtcgcagt agtcgaggac ggcctggcgc
      901 gcctgggcgc gcgcggcgat catttcctcg tcgctcaggt agcgacggcc gccgtcggcc
      961 agcgtttcgt acaggcgcgg cgaacgctcg taggtctcca ccgcggcgcg cgcctggcgg
     1021 caggcctcgg cgcggatctg ggcctgctcg gcctgcgcgg cggccacggc ctggcgctcc
     1081 gtgccgcgct gttcacgctg cgggtcgccc tcgagcaggg cgcgcgactc ttcggcggcg
     1141 cgcgccgggt cggtggcctt ggattcgacc ttgacccgct gggagcggtc cgagagcggc
     1201 cggtcgctgt agtgcaccac gccgcgctca tccaccgtgc ggtagacctc gttggccagc
     1261 gccggcgtcg cgagcatcgc ggccagcagg gcgggaagca ggaatctcat cacggttgac
     1321 ccccctagag cgccttctcg tcagtctccc cggtgcggat ccggatcgcc tgctcgaggt
     1381 cgtacacaaa aatcttgccg tcgccgatct tgccggtgcg ggccgtgttg acgatcgact
     1441 cgaggacccg ctccagcgaa tcgtcgggta ccgccagctc gagcttcacc ttgggcagga
     1501 aatccaccac gtattcggcg cctcgataca actcagtgtg gcctttctgg cgcccgaaac
     1561 ccttgacctc cgtcacagta atgccctgca cgccgaggtc ggcgagcgcc tcgcggacgt
     1621 cgtcgagctt gaagggcttg atgatggcgc agaccatttt catctcggtt ctccagattc
     1681 ctgtcatgcg agcggcgcag cggccgggca tggctgccgg tagttcgtcg cggaatgcga
     1741 gcctcgccgc agtctagcat tgcctcatcg ggcatgtaga ggcctgataa acaaaggccg
     1801 ggtgcggata gtccgccccc ggccggggtt gcggcgcggg gggcgcgccg cggttgtggc
     1861 caggcctcag tcgccggcct tggtgaactc cgggtaggcc tcgaggccgc actcgtgcag
     1921 gtccacgccc tcgtactcct gctgctcggt gacgcgcacg ccgaccgtct tcttgagcac
     1981 gaaccagacc agcaggctgg cgaagaacac ccaggcgaag atcaccgcca ggccgatgag
     2041 ctggcccgag acggtcgcat cgccgttgga gagcagcacc gcaagcacgc cccagatgcc
     2101 ggccgtgccg tgcaccgaga tggcgccgac cgggtcatcg atgcggaact tgcggtccag
     2161 caggacgatc gagctcacca ccagcaggcc gccgacggca ccgatgatca tcgccagcag
     2221 cggcgtcggc gagagcgggt cggcggtgat cgacaccagg ccggcgagcg cgccgttcaa
     2281 tgccatggtc aggtcggcct tgccgaacca cgcgcgcgcc agcagcagcg cagcgaccag
     2341 gccggccgcc gccgccaggt tggtgttggt gaacaccagc gccacgttgt tggccgactc
     2401 gacgtcggag accttcagct ccgagccgcc gttgaagccg aaccagccca tccacaggat
     2461 gaaggtgccg agcgtggcca gcggcaggtt ggacccgggg atggcattga cctggccctt
     2521 cgcaccgtac ttgcccttgc gcgcgccgag caccagcacg ccggccagtg cggcggccgc
     2581 gccggtcatg tggacgatgc ccgaccccgc gtagtcggag tagccgaagg tctcgccgag
     2641 gaagccgccg ccccaggtcc agtagccctg caccgggtag atcaccgccg tcatgacgac
     2701 cgcaaaggcg aaaaaggacc acagcttcat ccgttcggcg accgcgcccg agacgatgga
     2761 catggccgtg gccacgaaca ccacctggaa gaagaagtcc gagaggttgg agtagtaggg
     2821 tgcgtcgtcg ccgccggcga ccacctcggc gaccgcgttg tcggcgctgc cgagcatgcc
     2881 gaagccgggg atgatcccgc tgccgtcgcc gtacatgatg ccgtagccgc acagcatgta
     2941 catcaggcag gcgatcgagt agaggccgac gttcttggta aggatttccg ccgtgttctt
     3001 ggcgcggacg aggccggcct cgagcatggt gaagccggcg gccatccaca tgaccagcgc
     3061 gcccgacatc aggaagtaga aggtgtccag cgcgtagctg agttgcatta cttgttccac
     3121 gttctgcctc ccctgctcag acggcttcag ggccggtttc gcccgtgcga atccggatca
     3181 cctgttccag cggcatgatg aagatcttgc cgtcgccgat cttcccggta cgggcggtgt
     3241 tgctgatcac ctcgacgacc tgctcgagca ggtcgtcgtc gatcgccacc tccaccttca
     3301 tcttcggcag gaaatcgacg acgtactcgg cgccgcggta gagctcggtg tggccgcgct
     3361 ggcggccgaa gcccttcacc tcggtgacgg tgacgccctt gacgccgacg tccgccagcg
     3421 cctgccggac gtcgtcgagc ttgaacggct tgatgattgc tgtgacgagt ttcatgccgc
     3481 tcacctgagg tcgaaggtct tgccgatgct gaacatcagc gtgctctcgc tcgaaccggt
     3541 gcccgacagg tcgctgtcgc tgaccaccag gccgacgccc aggtcgaagc cggacacttc
     3601 cgcgccatag cccacttcga cgtagtcgcc ctcgaagtcc ttggagaagg agccgtaggt
     3661 ggcgtagaag ccctggtact cgacggtgcc ggcgatgaag ccgtagtcga gggtcgggcc
     3721 ggagaagttg tcgtagcggc cgagcgagta ctcgaggctg aacagcccgt agccggccgt
     3781 caggttgagc tcgtggtaag tgtcgtcgaa gtcgtcggtg tagaagtagc cggtgtagcc
     3841 gatcccgtag ctgaagtcgc cgatgctgcc gccatagccg gcgtacaggt cgtactcgag
     3901 gccttcgccc acctcggcgg cccaggtgcc cacgtagaag ccggagtttt cgtagtcgag
     3961 gccggcgaag gcggcggcgt cgttctgctt gatgccgcgg tagacgtagt cggacatcat
     4021 gccgatgttg gcgctaaaat ccgacgccag ggcgggttgt gcggtgccca gcgcgagcgc
     4081 gacgagcagg taacgggatc gacccatcgt tgtatcctcc gagtgtgttg caggacccgg
     4141 ccgtggagcc gcggttgggt tgtgcaaagc acaatgcgtg ccagccttga aataacagag
     4201 acttgcgtga tttcaggccg gccgggaggg gctgttcgca ccgtcgtggt gcatggctgc
     4261 agggggtgct gccccacggc ggggcgcgag gaagggtgat ggataccaag acgctgaaca
     4321 tcgacgagct cgcccgccgg ctggcggatg cgctgcccag cggcctgaag gcgctgcagg
     4381 ccgacgccga gcagaacttc cgcgcggtgc tgcaggccgg ccttgcgcgg ctcgacctgg
     4441 tgacccgcga ggaattcgac gtgcaggccg ccgtgcttaa gcgcacccgc gagaagctcg
     4501 ccgcgctcga gacgcggctc gcggcgctgg agcaggcacc ggagcgcacg gctgactagg
     4561 gccgccccgc caatcccgtc atagccgctg gaaagcggcc gttcccgcgg ccgtcggcgc
     4621 catgcacgct ggccggacgt tgccgggaga ttccgcatgg cgatggcgac cgtgctgagc
     4681 cgcgcgcagt gcgggctcga cgccctgccc gtgtcggtcg aagcctacgt cggcggcggc
     4741 ctgccgggct tctcgctggt cggactgccg gcgacggcgg tgcgcgagag ccgcgaccgg
     4801 gtgcgggccg cgctgctcac ctgtggcttc gatttcccgg atggccgggt gatcgtcaat
     4861 ctcgccccgg cggacctgcc caaggagggc gggcgcttcg acctgccgat cgcgctcggc
     4921 gtgctggccg cgtcgggcca ggtgccgcgc aacgggctga aggttacgga gttcatcggc
     4981 gagttgtcgc tcaacggcga tctgcggccg gtgcatgccg cgctgtgcgc agcgatcgcg
     5041 acggcgcgcg acggtcgtgg cctggtggtg ccggcggcct cggccgcgga ggccgcggcc
     5101 gccggggagg gcgaggtgcg cgccgcggcg agcctgctgg cggttgcggc gcacctgcag
     5161 ggccggcagc cgctgccggt cgtggcggcg ccgccgcggg tgatggccgg catccaccgc
     5221 ggcccggacc tgtccgaggt gcgcggccag ctgcgtggac ggcgcctgct ggaactggcc
     5281 gcggcgggcg gtcacaacct cttgttcgtc ggcccgcccg gcaccggcaa gaccatgctg
     5341 gcgcgacgcc tgccgggcct gctgccgccg ctggacgcgc gcaccgcact gcgggtcgcg
     5401 gcgatcgcat cggcagccgg caagccgttc cggcccgacg actggggccg ccggccgttc
     5461 cgcgcgccgc accactcggc gtcgtcggtg gcgctgaccg gcggcggctc catcccgcgc
     5521 cccggcgaag tgaccctggc gcaccacggc gtactgttcc tcgacgagtt gccggagttt
     5581 cgccgcgacg cgctggagtc cctgcgccag ccgctggagg acggcgagct gaccatcagc
     5641 cgcgccgctc tccagcgcac cttcccggcg cgcttcatgc tggtggcggc gatgaacccc
     5701 tgcccctgtg ggcacctcgg cgatccgcgc ggcaactgcc ggtgcagcgc cgaggcggtg
     5761 cgccgctacc gcgcccggat ctcggggccc ctgctcgatc gcgtggatct cgtggtggag
     5821 ctgccgcgcc cgagtgccga cgagctgctc ggtccgccgg gagaagaatc ggcggaggtg
     5881 cgcaagcgcg tgctggcggt gcgccaggcg agcctcaagc ggcaaggctg cgtgaacgcg
     5941 cggctggagg gcaaggcgct ggaccggcat gcgccgctcg gcccggccca gcgggcgctg
     6001 ctggcgcgct cggcggaccg gctcggcttg tcggcgcggg cgcagacgcg cgtgcggcgg
     6061 gtggcgcgca ccatcgcgga cgccgccggg gcggacgcca tcgaagagaa acacctggcg
     6121 gaggcgctgg cgtgccgggg tatgccggag tgaacgggcc ccgtcagacg aagcgcacga
     6181 ccaggaacag ccccaccatg gcgatgccga gcgccagctt ggcggcgatg ccgatggcca
     6241 gcccgacggt tgcgccccag ccggcccggc tggcggaggc caggtcgcgc tgggcagaca
     6301 actcgcccag cacggcgccg acgaagggcc cgagcagcag gccgggcagg ccgaagaaga
     6361 tgcccacgac ggcgccgagc gtagcgccga ccacggagcg cggtgaggcg ccgtagcgcc
     6421 gcgcgccgaa agcgccggcc accaggtccg caagaaaggc cagcaccgcc attacgccga
     6481 gcgcggtgag cgtgcctgcg ccgacgtgca cgaagttctc cgcccaggcg gccagcagca
     6541 ggccgaggaa cagcagcggc ggcccgggca gcgcaggcag taccaggccc gccaggccgg
     6601 ccaccaccag cagcacggcg agaatccata gcagcactgt catgggcgcg actatagcgg
     6661 cgaccatttg cggcgcctat gccggcattt gcccggctga gtccgccgcg ctgattcggg
     6721 tatagtgccg ccctcccgcg ccctgccagt ccggtcaccg atgtcgctga accccgccca
     6781 gctcgaggcc gtgcgctaca cggccggccc cctgctcgtg ctcgccggtg cgggcagcgg
     6841 caagacgcgc gtgatcaccg agaagatcgc cgcgctggtg gccggcggca tgccggcgta
     6901 ttccatcgcg gccgtgacct tcaccaacaa ggcggcgcgc gagatgcgcc agcgcgccgg
     6961 cgcgctgctg gacgcggaga ccgcgaagca gctgcgcatc tcaacctttc acacgctggg
     7021 gctgaacatc ctcaggcgcg agtacggcaa cctcggctac aagcgcggct tcaccatctt
     7081 cgacgccaac gacagccgca acctggtcaa ggagctcttg aagaacgaca acgccgacac
     7141 cgagggcgcc atcgaggctg cctgcgcggc gatctcggcg tggaagagcg agctggtcgc
     7201 gcccgaggcc gcgctcgccg ccgcgaccga tgagctggaa caggcgcgcg cccgccagta
     7261 cgccgcctac cagcgcgcgc tgcaggccta caacgcgctg gacttcgacg atctcatcgc
     7321 ccggccggtg ttcctgttcc gcgaactgcc ggctgtgctg gcggcgtggc aggaccgcat
     7381 caattacctg ctcggcgacg agtaccagga caccaacttc gcccagtacc agctgatggg
     7441 ccagctggtg ggcgcgcgcg gcgcgttcac ggtggtgggc gacgacgacc agtccatcta
     7501 cgcctggcgc ggcgcacggc ccgacaacct caagagcctg gccgccgact acccggcgct
     7561 gaaggtcatc aagctggagc agaactatcg ttcgtccggg cggatcctcg cggcggcgaa
     7621 cggcctgatc gccaacaacc cgcacctgtt cgacaagcag ctgtggagcg cgcacggccc
     7681 gggcgaccgc atccgcgtgc tggtggcgcg cgacggcgcc gacgaggcgg accgcgtcgc
     7741 catggagatc atgcgcatgc gcatggagcg cggcggcgaa tggcgcgact tcgcggtgct
     7801 gtatcgcggc aaccaccagt cgcgtgtgtt cgagcaggcg ctgcgggcgc tggagatccc
     7861 ctactcggtg accggcggca tctccttttt cgatcgcgcc gaggtcaagg acgtgctggc
     7921 atacctgcgc ctgatcgcca acccggacga cgacgcggcc ttcctgcgca tcgtcaacgt
     7981 gccgcggcgc gagatcggcg ccgccacgct ggagaagctc ggcgcctggg cctcgagccg
     8041 cgggctcggc ctgctggccg ccagcctcga ggcggggctg cacgccgtgc tgccggcgcg
     8101 caccgtcgag cggctgcacg acttcgcgcg gcgcatgatc gagctgggcg acgaggccga
     8161 ggacggcgcc cgcgtcgatg agctggcgcg cgaactgatc gaggagctcg gctaccgcga
     8221 ctggctgttc gagaatgccc gcgatccgcg ccaggccgag accgccaacg aaaacctcgc
     8281 cgagctgctc gactggctgg gccgggcctc acgcagcggc gacgagccga cgctggccga
     8341 cgcgctgcgc cggctgtcgc tgatggacat gctcgaccgc cagtcggagg acgagacgga
     8401 cgaccgcgtg cggctgatga ccctgcatgc ggccaagggg ctggagttcc cgacggtgtt
     8461 cctggtcggc tgcgaggaag agctgctgcc gcatcgcgcg aacctcgtgg gaccgggcct
     8521 ggaggaggag cgccggctgg cctacgtcgg catcacgcgt gcccggcagc ggctgttcct
     8581 gacttatgcg cgccggcgca agcgcttcgg cgagacgctg gagtgcgagc cgagccgctt
     8641 cctggaggag ctgccggccg aggcgctcga atgggaaggg cgcgtcgagc tcagtgcaga
     8701 agaaacgaag gcccgcgggc agagcgccat cgcgggcctc cggtcgctgc tggagtgaag
     8761 gcgaaaactg gggtcaaaat tggggtcaga cacctatttc gcgaacgaaa taggtgtctg
     8821 accccaattt tgaccccaat ttatgaccgc cgatttttca gccgccgagc tggtcgacca
     8881 tgtattgcac ggcgtcgcgc acctgctggt cggtgagcga cgagttgccg ccgcgcggcg
     8941 gcatgtagcc ggcctggccc tggaagccct cgatggcgtg ccgcaccagc gtgtccatgc
     9001 cctggtcgac tcgcccggcc caggcgtcgg cgtcgccggt ctttggcgcc ccggcgatgc
     9061 ccgcgccatg gcaggccgtg caggccgcct tgtagacggc ttcgccgtcg agatccgcgg
     9121 ccgcctgcgg cgccgctgcg ggcggctcca tcgtggccgg cgccacgccg ccagggtcac
     9181 gcagcgcgct gttgtcgcgg ccggcgactg ccacctggcc gaccggacgg atccgctcgg
     9241 cgacgcgctg ctggtacatg gggtcttcct tgatgtgcac cgcctgggtg cggttcgaca
     9301 tcgcggtcgc caggaagtag atggcgatgg tgaccaccac caggatgccc agcaccagca
     9361 tgaaggtgtc gaagaacttg cggtcgtcct ctgcgctcaa ggtcgtgccc tctcggattt
     9421 cggaagccgg cgcccgtcca gggtcccggg aagcggggga ttataggggc ttgccgcagg
     9481 cccgccaaac ccgccggccg gcccggtgtc gtcatctgcc ggcggaggcg aagcctcgca
     9541 ggatatcgag ggcgagtccg ggccgggcac cgatgctggc ggcgtcgaag ccgtggctga
     9601 ggaacggagc aaagcccggc agggcctgcg cctcgacctc gccgaggcgg cggaaaccca
     9661 tgtgccagcc cgcgaaactg cgcgcctcga tcggtgtgcg ctccagcagg aacaggtcgg
     9721 tatggcgtgg atcccgctcg atccgcccat aggtctcgtc gaccgcggcc tccgcgcctt
     9781 ccagcacctg catgaagctg ccgttgaggt acagcagcat gccggtgacc gagtgtgcgg
     9841 cgttgtgccg gacgctggac gcgagaatcc tgtccagttc ggtcgtgtcg tattcctgcc
     9901 gcgccgtgct tacgtagatc agctggatga gtgtcatgac ggccccgatt gtgcctccgg
     9961 cgccgcatgg acgctaatgg cggttgccgg tatcattgcc gcccttcccg tgtgcgcccg
    10021 tagctcagct ggatagagcg ctgccctccg gaggcagagg ccagaggttc gaatcctctc
    10081 gggcgcgcca atacacaaag acccccgaag cggggttttt gtgtattcgc cctttccggg
    10141 tgattcgaac ctctgaccaa ctcgaccggt tcgtcgtccg gctgccaggc cgggcgggcg
    10201 tcccgacggg gcgccgcatc ctctcgggcg ctgcgaccag ccggccgaga cgccccgcag
    10261 cacgggacgc ctcggtctcg gtcaagcgac attgaagagt ggccgacttc ggccctgttc
    10321 gttggctgaa tgcggcagcg gtaacagaca ccgcaccgcg ccaggcggac ccggcgtcta
    10381 gcccttcttc gcccagacgt tgcaccagcc gggtcccgcg acctgcttgc ccggaaacag
    10441 gtcacacggc gcccaggcgt cagcctgggt gcccttgaag tagcggcagt tgtaacagaa
    10501 ctgatctgcg ctcggccgtg ccgacttttc agcatcatga acatacttca gcgacttggc
    10561 ggtgggatcg tcctcagaca ccctggagtc tgccgcctgc agtctggaaa ctggcagcag
    10621 ggccgaaccg gccagtgccg ccagcccaag ggctgagcct cgcaagattg accgtcttga
    10681 cttggagaaa tcgcgcttca tgaatggctc ctcagaagaa agcgccgtcc ggggcgcacc
    10741 ccggacggca aatgtggaac tgttgctgtg tcagaacgaa atgtcggctg tcacattgat
    10801 gacccgcccc gtgttcgcag agcccgtcag cgagttgctc tggttgttgc tataggccag
    10861 gtagatcagg aaattctcca cgggcttgta cgcgagcgtc aggttccagg aaatcttcgt
    10921 gtcgatgtcg tacttcgtgc cggcgagatc cgtgaactcg taagtgaagc tgtcctggat
    10981 cgacggcttg atgaagtacg tatggttgta gccgatgacc gtctggacgc cccaggtgtt
    11041 gcgcttggtc ttggcgccgt caatgtagtc ctcggagttg aagccgacgc ggaagacggt
    11101 ctcgaagctc cacgggcccg tggtgccctg gctgttgaag aacgagccga cgttctggcc
    11161 gccccagcga acctccacgt cgacggacgt ggcgctgtcg atttcttccg ccaggtaggc
    11221 gcagctgccc gccggtcgcc gtcccgcgac gccggggcag gggtcaccgg gcgggcggcg
    11281 cgtgttgctg acgccgccga ccgcgtcccg gtactggaag gaccgcgatg acgcctgcgc
    11341 gccgttggcc gtcacgagcg gcatgacgtc gctacccgtg cgggccgcga tgttgatcca
    11401 gagctcgttg tcattgcggt tgcgcaacgc gtagccgaag aacccgagaa ggttgttttc
    11461 atcccagccc atgttcgtgt cgccggtgtt catgcccagg gcccagatcc agcgatcgta
    11521 catccagccg taggcggtca gcgtaccctt gttcggatgc gtgtagccgc cgatcacgcc
    11581 gcgattgaga tagctcggca gcccgggata gggcccgaat ccgaacaatt catagatcga
    11641 ctggttgttg aaggccaggc cgatcgtgta gtccatgttt tccgggttga tgatgtagcg
    11701 gaagtcgaat tcctcgtacg aggtgtcggc gatgccccat tcgttgtctt cgctcgtgaa
    11761 cggaacgatg tagaactcgg tccagacacc gaagtggtcg ccgatcggcc cgaggaacgg
    11821 gaagaaggcc agccgtgagg tcgggttgct ggtggcttcg ccccagtccg ttgcggtcgc
    11881 gcccttggtc ttctggtagg gcgtgtactg catgcgaccg ctgaaggtgg tcggcagcat
    11941 cgtcaggaac tcgccgaggt cgtccggcgt gccctgttcc atggcgcgct cctgggtgtg
    12001 gtgcggtttg ccagggagtc gatagcccac cgcgttgaac ttcttaccgg taaaggtgaa
    12061 gttcggtgtt ggcgcaccgt gcaaggtatg gcaggagttg cagttcatgc ccgtctggcg
    12121 cgtgaagccc gccacggcat aggcctcgtt cgcagcgaac aaggcgatga acagtattcc
    12181 cgccgggccg agcaggccct tgttcaagaa tctcatgaca gcttcccctc tttgtcgttc
    12241 tgtaggaaat ccacgcaatc aggtgtcggc tggccgcagg ctattcctgc aggtcaccct
    12301 tcaggtaagc cacgagcgcc tcggcctcgc cgccgcggat gaaatgaaac gccggcatcc
    12361 ccatgtacgc ggtgtgcgta cggttgccgt agttcctgcc cttctggatg acctcgatga
    12421 tgatttgtgc aggtgcgttc tgcacaaagg cattgccctt cagggccggt ccgaatgcgt
    12481 agatgccgct gccgtcctga ccgtggcacg cagcgcagtt cgcgtagtaa cgccacttgc
    12541 cgtcaaattt ctcctctgcg gatgctttgg acgccgagat ccccacagcg cccagggcga
    12601 gcagacttcc cagtgccaca actccgagtc cgatggcttt catttttttc ccccaggaat
    12661 ttgtacatgg tcctccacag tggaatcagt tagcccgcta attgatccaa gtcaaaaacc
    12721 gacaggccga aacaggctgc atgtgggctg cggttcccaa aacgcagcga attgacctgt
    12781 ttgtctaggt tgttttcaca aatagttaac gctaccttca tcaggggaaa ttgctgcatc
    12841 caatgaattc tgtgctgcac cgcgaaacgg actttgctgc gacatcgcgg gggtccgaca
    12901 ttggacgagc gggagcgtta ctggcggcgc gtgaaaggca tgctcatgct gcagaccggc
    12961 gtgccgcagg ccgaggtggc ccggcggctc ggggtcacgc gggcggccgt ctccggatgg
    13021 aaggggcggc tggcgggcgg ggagaccccc cacacgcttc ctaagcatcg cggccgggtc
    13081 cctcgtctca gccacgaaga ctgccgggtg ttgcgcgagc tgctgcagcg ggagagtgaa
    13141 ggcggacgac gcctaaagct ggaggagatt ggtcgcctga tcgctgcgca cttctccatc
    13201 gagttcagcg tcgcgcaggt gtcccgaatt gcgcatggca tcgggtggag cgtgcaatgg
    13261 gggcgcctgg taccgccgcg gtccgaaagg tttcgagcag gctttcggcg cgcacaacag
    13321 gccaggttgg ccaggctggc aacattgatg aacaatattg cggtcgtaca cgatggagat
    13381 gagcaacttg ggcgccagga cccgcagtaa cggaacgggc aaatcgccgg cactgccacg
    13441 gaccatggcg cccctcggcc gcgtccgggc tggctggcag ctgcgtagcg gtttgctact
    13501 cggaatcgtc ctgctgctgg ccttcgtcca cccccccgcg caggccgact ccgaggccct
    13561 cggcgacccg ctgcatcgct acgacgagca cacgccgagc gccaacacga tgcagcgcgc
    13621 gcgccgcgcc taccgggcca acaggcaccg cgccgccttc gagtactaca aggttgcggc
    13681 cttctgggcc gacaagctcg cgcagtacaa cgtcggcgtg atgtatctgc agggccaggg
    13741 cgtcgagttc gacccggtgc gcggctgggc gtggctggag ctctcggccg agcgcaagta
    13801 ccccgacatg gtggcggcgg ccgaccagct ctccgcgctg ctcgacggcg cacaacgcca
    13861 ggccgcgcga cgtatcctcg aggaggaact gctgccggag tacggcgacg acgtcgccat
    13921 cgagcgcacg gcgaaggaaa tgcgccgcga gctcaggcgg ggcacgggct cgcgtctcgg
    13981 cagtcgcgcc tcgctgtcgg cggtgaccat catcgacggc aatggcatga cccgccgcgg
    14041 cgacgagttc tacgaccccg ccaagtggga tttcgaaaac atcgtgcgct acgagacgcg
    14101 gctcatgttc cagctggccc gcggtcgcgt tgaagcgggc gagctggtca tcgtcgagga
    14161 cgaagaccag tagccggcat gcgtcggcgc gccgccatcc tcgtcgcctg cgggctgctg
    14221 ctggtgttca tcgtgctgtc gatgacccgc gacgaggaat acggccgcga ctggctgtcc
    14281 tggctgcccg gccagtcgcg ccacgtgttc gcccacgtgc cctgcggcat cccggtgcat
    14341 tacgcgctgg cggcggtcga ccctggattc ggcctggatc gcgacacggt gatggcggcg
    14401 ctggtcgaag cggtgagcct ctggcagccg ccgtccggcc cgctcctgtt cctcgaatca
    14461 gaccacgcac tcgcgattca ggtcagcctg cgctacgacg agcgccagca ggcggccaac
    14521 acgcgcagtt ccctgcgtca tggcctggag cgggaccgcg gcgccttcga ggatgacgag
    14581 gcgatgctcc tgcagtgggg tgaacggatc gatgcggccc gcgcatccca tgagcgcgca
    14641 gcggcggcgc tggcagcccg cgcgcgggcc tacgagcaag atgtgaccgc ctggaacgcc
    14701 ggccgcggcg agcgtaccga gacacgccgg caggcgctcg agactgagag cgccctgctg
    14761 cgcgacgcgc ttgccgacct cgagcgcgcc ggcctggcgc tcaacgccga cgtcgaagcc
    14821 tacaaccgcc gcgccgccga cctgcggcag cgggccgacg atttccaggc gcgggtggcg
    14881 cgctacaacg cggcgtcgtc cgcctcgccg gtcgagtcgg ggcgctacag ctacgcgcct
    14941 ggcgagggcc ggcgcatcga ggtgtaccgc gccgagagct ttgacgaact ggtgctggtg
    15001 ctggcccacg agctcggcca cgcgctcggc atcggccacg tcgacgagcc gggcgcggtc
    15061 atgcatgcca tgctgcacga cggcggcctg ctgcagtccg acccaacccg gccgctcgaa
    15121 ttgtccgcgg ccgataaggc cgcgctggtc gcggtctgcg ggcagcggct gcgtccgtag
    15181 cagaaggacc cgcctgacgg cgccgcggtc cgctcgcata tactgcggcg gtcgcttgcg
    15241 ggagaggact gcgtggaaga ggtcgagccc gggctgctgt cggcgctgct gctgggcggg
    15301 cacatgctgc tggtcgccgg gctggcggtc cgcatcctgt tccgtcacct gccggtcggc
    15361 accacgctgg cctggctgat cgtgctgttc gtgctgccgg tgttcgggcc
//
LOCUS       DAOMPG010000034        15173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1696_length_15173_cov_4.290713, whole genome shotgun sequence.
ACCESSION   DAOMPG010000034 DAOMPG010000000
VERSION     DAOMPG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15173
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1696_length_15173_cov_4.290713"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLS34_03105"
     CDS             <1..644
                     /locus_tag="PLS34_03105"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP86493.1"
                     /translation="PQQLSCSAVALGGEPGALLLEFREVEQRRRADRERRLLDSAKAT
                     RELVRNLAHEIRNPLGGLRGAAQLLERELPTPELREFTRIIISEADRLTALTEGLLGP
                     VPHRSVGASTAVFAALGLLGSLGWAGRRHSVQGWIYRFGPIVAAVALLAYTGAGGERT
                     DVGAHIWGFAAGLGAGWLAAFIPRAVLQRAVVQAFAGLAAVGALVAAWLLALA"
     gene            complement(676..2130)
                     /gene="putP"
                     /locus_tag="PLS34_03110"
     CDS             complement(676..2130)
                     /gene="putP"
                     /locus_tag="PLS34_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HRP86494.1"
                     /translation="MVETGSAVTLTFVVYLIVVLAIGVVAWRSTANLRDYILGGRRLG
                     RWTTALSAQASDMSGWLLLGLPGYAYLAGLESLWLVGGLLVGTWLNWRLTASRLRTAT
                     EAYGDALTLPEYLQRRFADPYGLLRLLGAAVVLLFFTFYTASGLVAGGKLFEAVFHLP
                     YVWAVAAGAGAVLAYTFIGGFLAVSWTDALQGMLMLVALVLVACVGVAVTGGFDGLAN
                     GLEAANPALLSAFTDSAGAPLTFIAVASLLGWGLGYFGQPHILARFMAVRSAGELTAS
                     RRIAVGWVTIALLAATLVGLAGAAWLDPPLQGADSEKVFMAMAAALLHPVVAGICLAG
                     ILAAVMSTADSQLLVASSAVSEDLYRDLLRPNAAPGELLWIGRFAVIVIAIGAFLLAL
                     DPDSKVLDLVAYAWAGFGASFGPTILASLYWRRMSLAGAYAGILTGGVTVVVWRSLSG
                     GVFDLYEIVPGVVLSALAVAIFGRLGKPAPMPGS"
     gene            2244..3323
                     /locus_tag="PLS34_03115"
     CDS             2244..3323
                     /locus_tag="PLS34_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP86495.1"
                     /translation="MSSPALRILAGPTALSLLRADGFDPDAFSVMAGASGGAKWLVLC
                     GLDHALAEHFVARRRSKLFLVGSSIGAWRSCCHAQADPLAAFARFEEAYLDQRYSAHP
                     SAAEVSRVTAGILDALLGERGVTEILAHPLLRLNIVADRARGVAAVETPWVQKLALGA
                     AAMANIVSPAAVRMFFARTLFHDPRDAPPFMDPRRLPAVNVPLSPDNLAPAVMATGAV
                     PLVLRGVPAIPGAPPGLYRDGGIIDYHFNCDLSGGDGLVLYPHFYPHVVPGWFDKMLP
                     WRRAGAAALDRTVVLCPSDAFVAGLPGGKIPDRQDFWNFDAAERLRRWRFALQASRRL
                     GEEFAELVAAGRLPARVEPLAQVRD"
     gene            3397..3864
                     /locus_tag="PLS34_03120"
     CDS             3397..3864
                     /locus_tag="PLS34_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRP86496.1"
                     /translation="MSLAKTVDYALSLTRVDYQILRHHHTSTSLGSARTAHVDPAELA
                     KAVVLKDQIGPLLAVVPASERLDLDRLRATLRRPGLDFLPEDQLDALFYDCDKGAIPP
                     LGPDYRVPTVVDTHLRDADNVYFEAGDHEDLVHVNQTSFRALMRGAEYLDIVK"
     gene            3984..4700
                     /locus_tag="PLS34_03125"
     CDS             3984..4700
                     /locus_tag="PLS34_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012156509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP86497.1"
                     /translation="MARKPRFEVVGLPQHVVQRGNNRQVCFRDDEDRRRYLADLEEAL
                     HAHDVELHAWVLMSNHVHLLATPRTSRGISRMMQKLGSRYVRWFNDRHERSGTLWDGR
                     FRASLVETDSYLLACMRYIELNPVRANMVRSPGEYRWSSFAANALGWPDPLIVPHPTY
                     LALRATVNQRCAAYRRLCSQALDEGMLADIRDALASELVLGSEKFKTLVESATARPAR
                     RRRGGRPPREPEAGDSETGV"
     gene            4825..6636
                     /locus_tag="PLS34_03130"
     CDS             4825..6636
                     /locus_tag="PLS34_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP86498.1"
                     /translation="MKPTDTQHPDWFHRVVDCQWACPAHTDVPEYIRLIAQGRYTDAY
                     MVNRESNVFPGVLGRVCDRPCEPACRRGRVEAKPVAICRLKRVAADHKGEIADRLPQI
                     PQQKNGRRIACIGAGPASLTVANDLLPLGYEVTIFEALQKHGGLMRSNIPSFRLPAET
                     LDEEIGVIEGMGAEIRLGHRIESMKALLEEGWDAVFVGSGAPRGKDLELPGRKEAAAN
                     IHIGIDWLESVAFRHIDRIGKRVLIIGVGNTAMDCCRSSLRLGAEDVKVMARKPRGFF
                     KASDWELEDAEAENVEIVVNHSPKAFVLEAGKLAGMLFELMEYELDERGRITGQRVAG
                     EKLYPCDDVVLAIGQENAFPWIERDLGIEFDQWEVPVVDPTTHASTRPGVFFGGDAAF
                     GPKNIIWAVAHGHQAAVSIHLHCEGRPLESRRPRQLRMEPRKMGLHEWAYNNDFDPRE
                     RRLVPHVELKKRFEKLDIEVELGFTAEQAAQEVERCLNCDVQTVFSAKLCIECDACID
                     ICPVDCLAILPDADEQTLRGGVKNPAVNPDQPFFVSSPLKHTARVMVKDENLCVHCGL
                     CAERCPTAAWDMQKSRVLIPYASDEEPGCPEKQRRSA"
     gene            6606..8429
                     /locus_tag="PLS34_03135"
     CDS             6606..8429
                     /locus_tag="PLS34_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRP86499.1"
                     /translation="MPGKAAPQRVNDFTLKLANVNGTGSASANGLLMKSFFRMGIPVS
                     GKNYFPSNIQGLPTWYEIRVSREGYRARSGRVDLVVAMNAQTYARDLAEVSPGGYLLY
                     DSTWPRSHVLSREDVTVLGAPFAQLCNEAFDGVRTRILMKNMVYVGTLAALLDIDMAA
                     LEQLAKDSFGKRPGLVEANMKAIMMGWDYARQHFDCPLPFRVEPMNATSGMVMMDGNT
                     TAGLGCVYAGATVGAWYPITPSTSLMDAYRSFCARFRADPETRERRYCVIQAEDELAA
                     IGMVLGASWAGARAFTPTSGPGISLMSEFIGFGYFTEIPAVIFDVQRVGPSTGLPTRT
                     QQGDILLAAYASHGDTRHPVLFPADPTECFEMAVQAFDLAERLQTPVFMLSDLDIGMN
                     DWMCRAPTWDDAYVPDRGKVLSAAELERIEKFRRYADVDGDGIPWRSLPGVHPKGAYF
                     TRGSGHNEAAGYTEDAGEYERLMERLKRKFATAATLVPAPVLQRADKKTSLGLVSVGS
                     CDDAVSEARDRLQAEGIRSDYLRIRAFPFNEDVQAFLDAHDRVFVIEQNRDGQLRTLL
                     INECRVDGRRIESILHYDGLPMAAGPLVDMVRARLEKGAAA"
     gene            8426..9481
                     /locus_tag="PLS34_03140"
     CDS             8426..9481
                     /locus_tag="PLS34_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRP86500.1"
                     /translation="MTYIAKPKVDHPGLTRNALGLSRRDYEGALSTLCAGCGHDSVTA
                     AIVQACWELSLPPHRMVKLSGIGCSSKTPAYFVSQAHGINTVHGRMPSVATGAAAANR
                     NLNYLGVSGDGDSLSIGLGQFCHAMRRNVRMLYIIENNGVYGLTKGQFSASADLGSKA
                     KKGEINLQPPIDPVLTALAAGATFVARSFSGDKGQLVPLIKAGLSHEGFALIDVLSPC
                     VTFNDHEDSTKSYAFSRQFYHPAVHTDFVPPADEIRAGYEQGDAMEVELHDGSHLALR
                     KVEADYDPTNRDGAFVKIHERMQAGEWLTGLLYVETGRQDMHGLNQTVAEPLASMPHA
                     ALCPGAEVLDKTLARYR"
     gene            9673..11505
                     /locus_tag="PLS34_03145"
     CDS             9673..11505
                     /locus_tag="PLS34_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRP86501.1"
                     /translation="MTPLEPHGIAIMLLVFGALVLFSRDRIPMETSALVVLGVLVAGF
                     ELFPYVAPDGQLVRATVFLAGFGNAALVTVMSLLVCTRGLEVTGALRPVADRLARLWL
                     RRPMLALLAALSLACGLSMFLNNTPVVAMLLPILVSVSLRTGQPASSMLMPMGYATII
                     GGMCTTIGTSTNLLVVEVATDLGMAPMGMFHFTPIALAGAALGLVFLWLVAPRLLPAR
                     TPPLPDTSPRVFEAVLRIVPGSAAVGMTLAQLHEQSGGKLRLGAVERGAGLFLARRPE
                     LVLAAGDRLHLRDTPEQLKHFEAMLGARLHDPAAPGPPPGRSGRPKASGQRMAEIVVT
                     RGSPLHQRTLSRVPFFAHHGLLPVAVHRARSPGAEVADIGEVYLRAGDIVLVQGSAEA
                     LDGVKRLGNVLVVDGSFDLPSARKAPVAIAIMVTVVALSAFGLLPIQVAALGGVLAMI
                     VTGCLDWRGALGALDRRLILVIVASLGLGHALTVTGGTRYIADLFASAAAPLPTFVAL
                     GGLMLVMALLTEVVTNNAAAVLGTPVAMFIAERLGVPPEPFVLAVLFGANMSYITPIG
                     YQTNLMVLSAGGYRFTDFYRVGIPLQIIMWLGLTVFLGLAYGLL"
     gene            11623..13458
                     /locus_tag="PLS34_03150"
     CDS             11623..13458
                     /locus_tag="PLS34_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRP86502.1"
                     /translation="MPPPPDPHGIAIMLLAVVALFLFSRDRIPMETSALVVLGILVVG
                     FQVFPYVSPDGRSLATGDFLAGFGNSALITIMALLICTRGLEVTGALQPVADRLARIW
                     LHSPMLALLVALVLAAGLSMFLNNTPVVAMLLPILISVAMRTGQPASSVLMPVGYATI
                     IGGMCTTIGTSTNLLVVEVSTQMGLAPMGMFHFTGIALSAAAFGILYLWLVAPRLVPA
                     RTPPMPDTSPRVFEAILHIGEDSGTVGLSLTELLARTEGKMKLGAIERGPGLFVARLP
                     SVTIRAGDRLHVRDTPEKLKRFETQLGATLYQGGDSPDGPAEGERTKEAGQRMAEIVV
                     TRGSPLHRRTLNTVPFFAHHRLLPVAVHRPRAPGADVTDIGDVLLRAGDIVLVQGSVP
                     ALDGVKRLGNVLVVDGSMDLPHTSKAPVAAAIVTAVVVSAASGLLPIGVAALVGVLAM
                     VISGCLDWRGALGALDRRLIIVIVASLGLGRALTVTGGTQYIADLFVVAAAPVPTIVA
                     LGGLMLVIALLTEVVTNNAAAVLGTPVAVFIAQRLGVPPEPFVLAVLFGANMSYLTPI
                     GYQTNLMVLSAGGYKFSDFFRVGTPLQIIMWLALTVFIGLSYGLF"
     gene            complement(13531..14214)
                     /locus_tag="PLS34_03155"
     CDS             complement(13531..14214)
                     /locus_tag="PLS34_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRP86503.1"
                     /translation="MNLLQRFRLARARRNCRLPVQSRLLEAPLPSPGSRVSELEIVAL
                     DFETTGLDMRQDRIIAAGWALLRGDRIIMASAREVRVCNGGAEGVGQSAVIHGITDSD
                     LDDADSVEAMLEHLLPDLAGRAIVAHAVSIERGFLAALLRRIGGVPLPNPFIDTMALE
                     RRLLEGSGGSARELHGDLTLDACRARRELPDHQRHSAGADAVACAELLLAQIDQLGGA
                     ARVKLRDLT"
     gene            complement(14211..>15173)
                     /locus_tag="PLS34_03160"
     CDS             complement(14211..>15173)
                     /locus_tag="PLS34_03160"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRP86504.1"
                     /translation="VPKLFELMLASGMRAEEVPRVLTMITDTVTRRLIKLAQDRLGPP
                     PAPFAWLAFGSQAREEQSLKTDQDNGLVYADDAPPETDAYFKALAEFVCDGLDACGYV
                     YCPGKIMATTDKWRQPLRGWLRHFADWAGMPDPEAVLRVSIFFDLRPVCGDRELAAQL
                     SRALGECGSGTGKAVFLSALARQAAGYDVPLGFFRRFVLESKGEHRETLEIKRAGLMP
                     LTDLVRVRALQGGVTVPGTQDRLARLVEAEKMGRKEADRLAGAHRLLAGLRVRLHAQQ
                     ARKGETPHNHLDVRAISHAERAALKDAFLVIRDAQKGLALDFPS"
BASE COUNT         2290 a         5277 c         5190 g         2416 t
ORIGIN      
        1 aaccgcagca gctgtcgtgc agcgcggtcg cgctgggcgg cgagcccggc gcgctgctgc
       61 tggagttccg cgaagtcgag cagcggcggc gcgccgatcg cgaacgcagg ctgctcgatt
      121 cggccaaggc cacccgtgag ctggtgcgca acctggcgca cgagatccgc aatccgctcg
      181 gcggcctgcg cggcgctgcc cagctgctcg aacgcgagct gccgacgccc gagctgcgtg
      241 agttcacccg catcatcatt agcgaagccg accgcctgac cgcactgacg gaaggcctgc
      301 tcggcccggt gccgcaccgc tcggtgggcg catcgaccgc ggtgttcgcc gcgctcgggc
      361 tgctcggctc gctgggctgg gccgggcgcc gccacagtgt gcagggctgg atctatcgct
      421 tcgggccgat cgtcgccgcc gtcgcgctgc tggcctacac cggcgccggc ggcgagcgca
      481 cggatgtcgg cgcccacatc tggggcttcg cagcgggcct cggagccggc tggctggccg
      541 cgttcatccc gcgtgcggtg ttgcagcgcg ccgtcgtcca ggcgttcgcc gggctggccg
      601 ccgtcggcgc gctcgtggcc gcgtggctgc tcgcgctcgc ctgatgggcc attggggaat
      661 gtccccaatg gcccgtcagg accccggcat cggcgcgggc ttgcccaggc gaccgaaaat
      721 cgccaccgcc agcgccgaca ggaccacgcc tgggacgatc tcgtagaggt cgaacacacc
      781 gcccgacagc gaacgccaga ccaccaccgt gacgccgccg gtgagaatac cggcgtaggc
      841 gccggcgagg ctcatgcgtc gccagtacag cgacgcgagg atggtcgggc cgaaagaggc
      901 gccgaacccg gcccaggcgt acgcgaccag gtccagcacc ttgctgtccg ggtcgagcgc
      961 gagcaggaag gcgccgatgg cgatcacgat gacggcgaag cggccgatcc acagcagctc
     1021 gccgggcgcc gcgttcggcc gcagcaggtc gcggtacagg tcctcggaca ccgccgagga
     1081 ggccaccagc agctgggaat cggccgtgct catcacggcc gcgagaatcc cggccaggca
     1141 gatcccggcg accaccgggt gcagcaaggc cgcggccatg gccatgaaca ccttttcgct
     1201 gtcggcgccc tgcagcggcg gatccagcca ggccgcgccg gccagcccga ccagggtggc
     1261 tgccagcagc gcgatggtca cccagcccac cgcgatgcgg cgtgatgccg tcaactcgcc
     1321 ggcggagcgc accgccatga agcgcgcgag gatgtgcggc tggccgaagt agccgagccc
     1381 ccagccgagc aaggaggcga ctgcgatgaa cgtcagcggc gcgcccgccg agtcggtgaa
     1441 ggcgctcaac agcgccgggt tggccgcttc gagaccgttg gccaggccat cgaagccgcc
     1501 cgtcaccgcc acgcccacgc aggccaccag cacgagcgcc accagcatca gcatgccctg
     1561 cagggcgtcc gtccagctga cggcgagaaa gccgccgatg aaggtgtagg ccagcaccgc
     1621 gcccgcgccg gccgccaccg cccacacgta gggcaggtgg aacacggcct cgaacagctt
     1681 cccccccgcc accagcccgg acgccgtgta gaaggtgaaa aacagcagca cgacggccgc
     1741 gccgagcaag cgcaacaggc catagggatc cgcgaaacgg cgttgcaggt actccggcag
     1801 ggtcagggcg tcgccgtaag cctcggtcgc ggtgcgcagc cggcttgccg tgagccgcca
     1861 gttgagccag gtgccgacca gcagcccgcc gaccagccag agcgactcca gcccggccag
     1921 gtaggcatag cccggcagac cgagcagcag ccagccgctc atgtcggagg cctgcgccga
     1981 cagcgccgtg gtccagcggc cgaggcgccg cccgccgagg atgtagtcgc gcaggttggc
     2041 cgtgctgcgc caggccacca cgccgatcgc cagcacgacg atcaggtaca cgacgaaggt
     2101 cagcgtgacg gcggaacccg tttcaaccat gcccggatgc taaagcaaat cggggtcatt
     2161 ccccattctt gcgccggcga attggggact gtccccaatt cccgtgatga aaaacgggcc
     2221 acggccccgt atcctctggc gcgatgtcct cccccgcgtt gagaatcctg gccggcccca
     2281 ccgcgctgag cttgctgcgc gcggacggct tcgatccgga tgccttcagc gtcatggcgg
     2341 gcgcatccgg cggcgccaag tggctggtgc tgtgcggcct ggaccacgcg ctggcggagc
     2401 atttcgtcgc gcgccgccgc agcaagctgt ttctggtggg gtcgtcgatc ggcgcctggc
     2461 ggtcctgctg ccatgcgcaa gcggatcccc tggcggcttt cgcccgcttc gaggaggcct
     2521 acctggacca gcgctacagc gcgcatccga gcgcggcgga ggtcagccgg gtcacggccg
     2581 gcatcctcga cgcgctgctg ggcgaacgcg gggtcaccga gatccttgcc catccgctgc
     2641 tgcgcctgaa catcgtggcg gaccgggccc gcggcgtcgc cgccgtcgag accccgtggg
     2701 tgcagaagct ggcgctcggc gccgccgcca tggcgaacat cgtctcgcct gccgccgtgc
     2761 ggatgttctt cgcgcgcacc ctgttccatg atccgcggga cgcgccgccc ttcatggacc
     2821 cgcggcgcct gcccgccgtc aacgtgccgc tgtcaccgga caacctggcg ccggcggtca
     2881 tggccaccgg ggcggtgccc ctcgtcctgc gcggcgtacc ggcgatcccc ggggcgccgc
     2941 cgggcctgta ccgcgacggc ggcatcatcg actaccactt caactgcgac ctgtcgggcg
     3001 gcgacggcct ggtgctctac ccgcatttct acccgcacgt ggtgccgggc tggttcgaca
     3061 agatgctgcc atggcggcgc gcgggcgccg ccgcgctgga ccgcacggtc gtgctgtgcc
     3121 cgtcggacgc cttcgttgcg ggcctgccgg gcgggaagat tcccgaccgg caggacttct
     3181 ggaatttcga cgctgcggag cgactgcgtc gctggcggtt cgcgctgcag gcctcgcggc
     3241 gactcggcga ggagttcgcg gagctggtgg ccgccggccg tttgccggcc cgggtggagc
     3301 cgctggcgca agtccgggac tgactctgga accagtccag atggctgccg gcagggccgc
     3361 tataattgcc gtgtacccat gcggcggagc cttccgatga gcctagccaa gaccgtggac
     3421 tacgcgctgt ccctgacgcg cgtggattac cagattctcc gtcaccacca caccagcacc
     3481 tcgctgggca gcgcgcgcac cgcgcacgtc gacccggccg aactggccaa ggcggtcgtg
     3541 ctcaaggacc agatcggtcc gctgctcgcg gtggtgcccg ccagcgagcg cctcgacctc
     3601 gaccgactgc gtgcgacgct gcgccgtccc ggtctcgatt tcctgcccga ggaccagctc
     3661 gacgcgctgt tctacgattg cgacaagggc gccatcccgc cgctcgggcc ggattaccgc
     3721 gtgcccaccg tggtcgacac gcacctgcgc gatgccgaca acgtgtactt cgaggccggc
     3781 gaccacgagg acctcgtgca cgtcaaccag accagtttcc gcgcgctgat gcgcggcgcc
     3841 gagtacctcg acatcgtcaa ataaccgggg tcagacaccc gtttccaatt tccggggtca
     3901 gacaccggtt tctccggcta ccctggtgcg cccatcaacg ttttcatgca aggaggcaca
     3961 tccacttgca tgagtgacgt tgcatggcga gaaagccgcg gttcgaagtc gtcggcctgc
     4021 cccagcatgt cgttcagcgc ggcaacaaca ggcaagtctg cttccgcgac gacgaggatc
     4081 gtcgccgcta tcttgccgac ctggaagagg ccctccatgc gcatgacgtc gagctgcacg
     4141 cgtgggtgct gatgtcgaac cacgtgcacc tgttggcgac accacgcaca tcccgcggca
     4201 tttcgcgcat gatgcagaag ctggggtccc gttacgtgcg ctggttcaac gaccgccacg
     4261 agcgcagcgg cacgctgtgg gacgggcgct ttcgcgccag cctggtggag acagacagct
     4321 acctgctggc gtgcatgcgg tacatcgagc tgaaccccgt gcgcgcgaac atggtgcgaa
     4381 gtccgggcga gtaccggtgg tcgagctttg cggctaacgc gctaggttgg cccgacccgc
     4441 tgatcgtgcc gcaccctaca tacttggcgc tgcgcgcgac ggtcaaccag cgatgcgcgg
     4501 cgtatcgtcg attgtgctcg caggcgctcg acgaagggat gctggccgac attcgtgacg
     4561 cgttggccag cgagctcgta ctcggatccg agaaattcaa gaccctggtg gaatccgcca
     4621 ccgctcgtcc ggcgcgtcgg cgtcgtggtg gccgaccgcc gcgtgagccg gaagcgggcg
     4681 actcggaaac tggtgtctga ccccgatttt ttgttgcttt ccgcgcgata gtggttaatc
     4741 ttcccagctg acgcccgcag cagacgggtg cgaacaggag gagagcgagc ggcgcagcat
     4801 tgctgcagcc ggtcgcaacg gctcgtgaag ccaaccgata cccagcaccc ggactggttt
     4861 caccgggtcg tggactgcca gtgggcctgc cccgcccaca ccgacgtgcc ggaatacatc
     4921 cggctgatcg cccaggggcg ctacaccgac gcgtacatgg tcaaccggga atccaacgtg
     4981 ttcccgggcg tgctgggccg cgtctgcgac cggccctgcg agccggcctg ccggcgcggg
     5041 cgggtcgagg cgaagcccgt tgcgatctgc cgcctgaagc gcgtcgccgc cgatcacaag
     5101 ggcgagatcg ccgaccggct gccgcaaatt ccgcagcaaa agaacggccg gcgcatcgcc
     5161 tgcatcggcg ccggtcccgc ctcgctcacg gtcgccaacg acctcttgcc gctcggctac
     5221 gaggtgacga ttttcgaggc cctgcagaaa cacggcggcc tgatgcgctc caacatcccg
     5281 tccttccggc tgcccgccga gacgctcgac gaggagatcg gcgtgatcga gggcatgggc
     5341 gccgagatcc gcctcggcca ccgcatcgag agcatgaagg cgctgctcga ggaaggctgg
     5401 gacgccgtgt tcgtcggcag cggcgcgccc cgcggcaagg atctcgagtt gcccggccgc
     5461 aaggaggccg cggccaacat ccacatcggc atcgactggc tggagtcggt cgccttccgg
     5521 cacatcgacc ggatcggcaa gcgcgtgctg atcatcggcg tcggcaacac cgcgatggat
     5581 tgctgccgca gctcgctccg gctcggtgcc gaggacgtga aggtgatggc gcgcaagccg
     5641 cgcggcttct tcaaggcctc cgactgggag ctcgaggacg ccgaggccga gaacgtcgag
     5701 atcgtcgtca accattcgcc gaaggccttc gtgctcgagg ccggcaagct ggcgggcatg
     5761 ctgttcgagc tgatggaata cgagctcgac gagcgcgggc ggatcaccgg ccagcgcgtc
     5821 gccggcgaga agctctatcc ctgtgacgac gtggtgctgg cgatcggcca ggagaacgcg
     5881 tttccgtgga tcgagcgcga cctcggcatc gagttcgacc agtgggaggt gccggtggtg
     5941 gacccgacca cccacgcctc gacacgcccc ggcgtcttct tcggcggcga cgcggccttc
     6001 gggccgaaga acatcatctg ggcggtggcg cacggtcacc aggccgcggt gtccatccac
     6061 ctgcactgcg agggccgccc gctggagtcg cgccggccgc gccagctgcg catggagccg
     6121 cgcaagatgg gcctgcacga gtgggcctac aacaacgact tcgacccgcg cgagcggcgc
     6181 ctggtgccgc acgtcgagct caagaagcgc ttcgagaaac tcgacatcga ggtggagctc
     6241 ggctttaccg cggagcaggc ggcccaggag gtcgagcgct gcctgaactg cgacgtgcag
     6301 accgtgttca gcgccaagtt gtgcatcgag tgcgacgcct gcatcgacat ctgcccggtg
     6361 gattgcctgg cgatcctgcc ggatgccgat gagcagacgt tgcgaggagg cgtcaagaat
     6421 cccgccgtca acccggacca gcccttcttc gtctcctcgc cgctcaagca cacggcgcgc
     6481 gtcatggtga aggacgagaa cctctgcgtg cactgcggac tgtgcgccga gcgctgcccg
     6541 acggcggcct gggacatgca gaaaagccgc gtgctgattc cctacgcgtc ggacgaggag
     6601 ccgggatgcc cggaaaagca gcgccgcagc gcgtgaacga cttcacgctc aagctcgcca
     6661 acgtcaacgg caccggttcg gccagcgcca acgggctgct gatgaagagc ttcttccgca
     6721 tgggcatccc ggtgtcgggc aagaactact tcccgtcgaa tatccagggc ctgcctacct
     6781 ggtacgagat ccgcgtcagc cgcgagggct accgggcgcg ctccggccga gtggacctgg
     6841 tggtggcgat gaacgcgcag acctacgccc gcgacctcgc cgaggtcagc cccggcggct
     6901 acctgctgta cgactccacc tggccgcgca gccacgtgct gtcgcgcgag gacgtcactg
     6961 tgctgggcgc gcccttcgcg cagctctgca acgaggcctt cgacggcgtg cgcacgcgca
     7021 tcctgatgaa gaacatggtc tatgtcggca cgctggccgc gctgctggac atcgacatgg
     7081 cggcgctgga acagttggcc aaggacagct tcggcaagcg tcccggtctc gtggaggcca
     7141 acatgaaggc catcatgatg ggctgggact acgcgcggca gcacttcgat tgtccgctgc
     7201 cgttccgggt cgagccgatg aacgccacca gcggcatggt gatgatggac ggcaacacta
     7261 ccgccgggct cggctgcgtc tacgcgggcg ccacggtcgg cgcctggtac ccgatcacgc
     7321 cctcgacctc gctgatggac gcctatcgct ccttctgcgc gcgcttccgc gccgatcccg
     7381 agacccgcga gcgacgctac tgcgtcatcc aggcggagga cgagctcgcc gccatcggca
     7441 tggtgctggg cgcgagctgg gcgggcgcac gtgccttcac gcccaccagc ggtccgggca
     7501 tttcgctgat gtcggagttc atcggcttcg gctacttcac cgagatcccg gcggtcattt
     7561 tcgacgtgca gcgcgtcggg ccctcgacgg gcctgccgac gcgtacccag cagggcgaca
     7621 tattgctggc cgcctacgct tcgcacggcg acacgcgcca cccggtgctg ttcccggccg
     7681 atcccaccga gtgcttcgag atggcggtgc aggccttcga cctggccgag cggctgcaga
     7741 ccccggtgtt catgctctcc gacctcgaca tcggcatgaa cgactggatg tgccgggcgc
     7801 cgacgtggga cgatgcctac gtgccggacc gcggcaaggt gttgtcggct gccgagctcg
     7861 agcgcatcga aaagtttcgc cgctacgccg acgtggacgg cgacggcatc ccctggcgga
     7921 gcctgcccgg cgtgcacccg aagggcgcct atttcacacg cggttcgggc cacaacgagg
     7981 ccgccggcta caccgaggac gccggcgagt acgagcgcct gatggagcgg ctgaagcgca
     8041 agttcgccac cgccgcgacg ctggtgcccg cgccggtgct gcagcgggcc gacaagaaga
     8101 cctcgctggg actggtctcg gtgggcagct gcgacgacgc ggtcagcgag gcgcgcgacc
     8161 gtctgcaggc cgaggggatc cgcagcgact acctgcgcat tcgtgccttc ccgttcaacg
     8221 aggacgtgca ggccttcctc gacgcccacg accgcgtgtt cgtcatcgag cagaatcgcg
     8281 acggccagct gcgcaccctg ctgatcaacg agtgccgcgt ggacgggcgc cgcatcgagt
     8341 ccatcctgca ttacgacggc ctgccgatgg ccgccgggcc gctggtcgac atggtgcgcg
     8401 cacggctcga gaagggggct gccgcatgac ctacatcgcc aagccgaaag ttgaccatcc
     8461 cggcctgacc cgcaatgcgc tgggactgag ccgccgcgat tacgagggcg cgctgtccac
     8521 gctgtgcgcc ggctgcggcc acgactcggt caccgccgcc atcgtgcagg cctgctggga
     8581 gctgtcgctg ccgccgcacc gcatggtgaa gctgtcgggc atcggctgct cgtccaagac
     8641 gccggcgtat ttcgtcagcc aggcgcacgg catcaacacc gtgcacggcc gcatgccctc
     8701 ggtcgccacc ggcgcagcgg cggccaaccg caatctcaat tacctcggcg tgtcgggcga
     8761 cggcgattcg ctgtcgatcg ggctcggcca gttctgccac gccatgcggc gcaacgtccg
     8821 catgctctac atcattgaga acaacggcgt gtacggcctg accaagggcc agttctccgc
     8881 ctcggccgac ctcggcagca aggccaagaa gggcgagatc aacctgcagc cgccgatcga
     8941 cccggtgctg acggcgctgg cggcgggcgc gaccttcgtg gcacgcagtt tctccggcga
     9001 caaggggcag ctggtgccgc tgatcaaggc aggcctgtcg cacgagggtt tcgcgctgat
     9061 cgacgtgctg tcaccctgcg tgaccttcaa cgatcacgag gactcgacca agagctacgc
     9121 tttcagccgc cagttctacc acccggcggt gcacaccgat ttcgtgccgc cggccgacga
     9181 gatccgcgcc ggctacgagc agggcgacgc catggaggtg gagctgcacg acggcagcca
     9241 cctcgcactg cgcaaggtcg aggccgacta cgatccgacc aaccgggacg gcgccttcgt
     9301 caagatccac gagcgcatgc aggccggtga gtggctcacc gggctgctgt acgtcgaaac
     9361 cgggcgccag gacatgcacg gactgaacca gaccgtggcc gagccgctgg cgtccatgcc
     9421 ccacgcggcg ctgtgccccg gcgccgaggt gctcgacaag accctcgccc gctaccgcta
     9481 gcagcggtcg aaaaccgggg tcagacacct atttcgaaaa ccggggtcag acacctattt
     9541 tctttggaaa ttaggtgtct gaccccggtt ttcgacccgg ttttcgaccc cggttcttga
     9601 aatttgcccg ccggcggcgg cgcgtgctag aagttgagca gtccgggccg ccgcccttcc
     9661 tggacgcttc gtatgacgcc gctggaaccc cacggcatcg ccatcatgct gctagtgttc
     9721 ggcgcgctcg tactgttcag ccgcgaccgt attcccatgg agacctccgc actcgtcgtg
     9781 cttggggtgc tggtcgccgg cttcgagctg ttcccctatg tcgccccgga cggccagttg
     9841 gtgcgggcga cggttttcct cgccggcttc ggcaacgccg cactggtgac ggtgatgtcg
     9901 ttgctggtgt gcacgcgggg gctcgaagtc accggcgcat tgcgtcccgt ggccgaccgg
     9961 ttggcgcggc tgtggctgcg ccggccgatg ctcgcgctgc tggcggcact gtcgctggct
    10021 tgcggactat ccatgttcct caacaacacg ccggtcgtgg cgatgctgct gccgatcctg
    10081 gtcagcgtgt ccttgcgcac cggccagccg gcctcctcga tgctgatgcc gatgggctac
    10141 gcgaccatca tcggcggcat gtgcaccacc atcggtacgt ccaccaacct gctcgtagtc
    10201 gaggtcgcca cggacctggg catggcgccg atgggcatgt tccacttcac gcccatcgcg
    10261 ctggcggggg cggcgctggg gctggtattc ctgtggctcg tggcgccgcg gctcctgccg
    10321 gcgcgcacac cgccgttgcc ggacacctcg ccgcgggtat tcgaggcggt gctgcgcatc
    10381 gttccgggca gcgcggcggt cggcatgacg ctggcccaat tgcacgagca gtctggcggc
    10441 aagctcaggc tcggcgcagt cgagcggggt gccggtctgt ttctcgcgcg ccgaccggag
    10501 ctggtactgg cggccggcga ccggctgcac ttgcgagaca cgccggagca gctgaagcat
    10561 ttcgaggcca tgctcggcgc gcgcctgcat gacccggccg caccggggcc gccgccaggc
    10621 aggtccggcc gtccgaaagc ctcgggccag cgaatggcgg agatcgtcgt gacacgcggt
    10681 tctccactgc accagcgcac gctcagccgc gtgccgttct ttgcccatca cggcctgctg
    10741 ccggtggcgg tgcaccgcgc ccgctccccc ggggccgagg ttgcggacat cggcgaagtg
    10801 tatctgcgcg ccggcgacat cgtgctggtg cagggctcgg ccgaggccct cgacggcgtg
    10861 aaacggctgg gcaacgtgct ggtcgtggac ggctccttcg acttgccaag tgcccgcaag
    10921 gcgccggtcg ccatcgccat catggttacg gtggtggcgc tgtcggcgtt cgggctgctg
    10981 cccatccagg tggcggcgct gggcggggtg ctggcgatga tcgtcacggg ctgcctcgac
    11041 tggcgcggcg cgctcggcgc gctcgatcgc cggctgatcc tggtgatcgt cgccagtctc
    11101 gggctcggcc atgcgctcac cgtcaccggc ggcacacgtt acattgccga cctgttcgcg
    11161 tcggcggccg cgccgctgcc tacctttgtc gcactcggcg gcctgatgct ggtgatggcg
    11221 ctgctgaccg aagtcgtcac caacaatgcc gcggcggtgc tcggcacgcc ggtcgcgatg
    11281 ttcattgccg agcggctcgg cgtgccgccc gagcccttcg tgctcgccgt gctgttcggc
    11341 gcgaacatga gctacatcac accgatcggc taccagacca acctgatggt gttgtcagcc
    11401 gggggctatc gtttcacgga cttctatcgc gtcggaattc cgctgcagat catcatgtgg
    11461 ctggggctga cggtattcct gggcctggcc tacgggctcc tttagcgagg cgccggacgc
    11521 cttgctccgg ccggccaccg ggcgtgccgg cttggccggg cgtgccatgc gtgctagaag
    11581 tgtcccgccc cgggcgagcc gcccgacgac tgcagaggcc caatgccgcc gccgccggat
    11641 ccccatggca tcgccatcat gctgctggcc gtggtggcgc tgttcctgtt cagccgcgac
    11701 cgcatcccga tggagacttc ggcgctggtg gtgctgggca tcctggtggt cggcttccag
    11761 gtctttccat acgtttcgcc ggacgggcgg agcctggcca cgggcgattt cctggccggc
    11821 ttcggcaaca gcgcgctgat caccatcatg gcgctgctga tctgcacgcg cggactcgag
    11881 gtgaccggcg cgctgcagcc ggtggccgac cggttggcgc ggatctggct gcacagcccg
    11941 atgctggcgc tgctggtggc gctggtgctc gccgccggtc tcagcatgtt cctgaacaac
    12001 acgccagtcg tggccatgct gttgccgatc ctgatcagcg ttgccatgcg caccggccag
    12061 ccggcctcgt cggtgctgat gccggtaggc tacgcgacca tcatcggcgg catgtgcacc
    12121 accatcggca cttcgaccaa cctgctggtg gtggaggtgt cgacgcaaat gggcctggcg
    12181 cccatgggca tgtttcactt tacgggcatc gcactttcgg cggccgcgtt cggaatcctc
    12241 tacttgtggc tggttgcgcc acgactggta ccggcccgga cgccgccgat gccggacacc
    12301 tccccgcggg tcttcgaggc aatactgcac atcggcgagg acagcggcac cgtcgggctg
    12361 tccttgacgg agttgcttgc ccgcacggag ggcaagatga agctcggtgc catcgagcgc
    12421 gggcccgggc tgttcgtcgc gaggcttcct tcggtgacca tccgggccgg cgaccggctg
    12481 catgtccgcg acaccccgga gaagctgaaa cgcttcgaaa cgcagctcgg cgcaaccctg
    12541 taccaggggg gtgactcacc ggacgggccg gcggagggtg aacggaccaa ggaggccggc
    12601 cagcgcatgg cggagatcgt cgtcacgcgt ggctcaccct tgcatcgccg cacgctcaac
    12661 accgtgccgt tcttcgcgca ccacaggctt ttgccggtcg cggtgcaccg gcctcgcgcg
    12721 cccggcgcgg acgtcaccga catcggcgac gtattgctgc gcgccggcga catcgtgctc
    12781 gtgcagggct cggttcccgc gctggacggt gtgaagcgac tgggcaacgt cctggtggtg
    12841 gacggttcca tggacctgcc gcataccagc aaggcgccgg tggcggcagc catcgtgacc
    12901 gcggtggtgg tgagcgccgc ctccggtctc ctgccgatcg gtgtcgccgc gctggtcggc
    12961 gtgctggcga tggtgatcag cggctgcctc gactggcgcg gtgcgctggg tgcgctcgac
    13021 cgccgcctga tcatcgtgat cgtcgccagc ctggggctgg gccgggcgct gacggtcacc
    13081 ggcggcacgc agtacatcgc agacctgttc gtcgtggctg cagcgccggt gccgacgatc
    13141 gtcgcgctcg gcgggctgat gctggtgatc gcgctgctga ccgaggtcgt caccaacaat
    13201 gcggcggcgg tgctcggcac gccggtcgcc gtgttcatcg cgcagcgact cggggtgccg
    13261 ccggagccgt tcgtgctcgc ggtgctgttc ggcgccaaca tgagctatct cacgccgatc
    13321 ggttaccaga ccaacctgat ggtgctgtcg gcgggtggct acaagttcag cgatttcttc
    13381 cgggtcggca cgccgctgca gatcatcatg tggctggcgc tgacggtgtt cattggcttg
    13441 agctacggcc tgttctaggt aaaccgggaa aaccggggtc agacaccaat ttcgttcgaa
    13501 attggtgtct gaccccggtt ttcccggttt tcaggtcagg tcgcgaagct tgaccctggc
    13561 cgcaccgccg agctggtcga tctgcgccag cagcagctcg gcgcaggcga ccgcgtcggc
    13621 gccggcggag tggcgctggt gatcgggcag ttcgcggcgc gcgcggcagg cgtccagcgt
    13681 caggtcgccg tgcagttcgc gagcactgcc gccgctgccc tccagcaggc gtcgctccag
    13741 cgccatggtg tcgatgaacg ggttcggcag cggcacgccg ccgatgcggc gcagcaacgc
    13801 ggccaggaag ccccgctcga tcgataccgc gtgcgccacg atggcgcgcc cggcgagatc
    13861 cggcagcagg tgctcgagca tggcctcgac gctgtcggcg tcgtccaggt cgctgtccgt
    13921 gatgccgtga atgaccgcgc tctggccgac gccttccgcg ccgccgttgc agacccgcac
    13981 ctcgcgggcg ctggccatga tgatgcggtc gccgcgcaac agcgcccagc cggcggcgat
    14041 gatgcggtcc tggcgcatgt ccaggccggt ggtctcgaaa tccagcgcga cgatttccag
    14101 ctcggacacg cgcgagccgg gggagggcag cggcgcttcc agcaaccggc tctgcactgg
    14161 caggcggcaa ttccgccgcg cgcgtgcgag ccggaagcgc tgcagcaggt tcaagacggg
    14221 aaatccagcg ccaggccttt ctgtgcgtcg cggatcacca ggaacgcgtc cttgagcgcg
    14281 gcccgctccg catgggagat ggcgcgtacg tcgagatggt tgtgcggtgt ctcgcccttg
    14341 cgcgcctgct gggcgtgcag gcgcacccgc aggccggcca gcagccggtg cgccccggcc
    14401 aggcgatccg cctccttgcg ccccattttc tcggcctcca cgaggcgcgc gaggcgatcc
    14461 tgggtgcccg gcacggtgac gccaccctgc agcgcgcgca cccgcaccag gtcggtcagc
    14521 ggcatcaggc cggcgcgctt gatttccagc gtctcgcggt gctcgccctt gctctccagc
    14581 acgaagcgcc ggaagaaacc cagcggcacg tcgtagccgg ccgcctgccg agccagcgcc
    14641 gacaggaaca ccgccttgcc ggtgccgctg ccgcattccc ccagcgcccg gctgagctgt
    14701 gccgccagct cgcggtcgcc gcacactggc cgcaggtcaa agaagatgct cacccgcagc
    14761 accgcttccg ggtcaggcat gccggcccag tccgcgaagt gcctcagcca gccgcgcaac
    14821 ggctggcgcc acttgtcggt ggtggccatg atcttgcccg ggcagtagac gtacccgcag
    14881 gcgtcgagcc cgtcgcagac gaactccgcg agggccttga agtacgcatc cgtctccggc
    14941 ggcgcgtcgt cggcgtacac caggccgttg tcctggtcgg tcttgaggct ctgttcctcg
    15001 cgcgcctgcg agccgaaggc cagccacgcg aacggcgcgg gcggggggcc gagccggtcc
    15061 tgcgccagct tgatcagccg ccgcgtgacg gtgtcggtga tcatggtgag cacgcgcggc
    15121 acctcttcgg cccgcatgcc cgaggcgagc atcagctcga acagcttcgg cac
//
LOCUS       DAOMPG010000035        15096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1708_length_15096_cov_4.832790, whole genome shotgun sequence.
ACCESSION   DAOMPG010000035 DAOMPG010000000
VERSION     DAOMPG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1708_length_15096_cov_4.832790"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLS34_03165"
     CDS             <1..448
                     /locus_tag="PLS34_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRP86505.1"
                     /translation="PIEPEFYGEFIDKLGLDRERFVPHGFRPGRTDTTRWAELKAELA
                     AVFRTRSRDEWCALLEGSDACFAPVLGLSEAPAHPHNAARGTFIEVGGVAQGAPAPRF
                     SRSAPAMPEAPHRPGADTRAVLGEAGYDPTALAGLEAAGVIPEEQD"
     gene            452..1252
                     /locus_tag="PLS34_03170"
     CDS             452..1252
                     /locus_tag="PLS34_03170"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRP86506.1"
                     /translation="MSEQRVTIERRDHVAVVTLARPDKRNALDPAMFDALDGAARELS
                     ADHELRAVVLAGAGKSFCAGIDLAAFAAGEAVIRKLLARRDGEAANLAQRVGWGWREC
                     PVPVIAALHGEVFGGGLQVALGADLRIARPDARLSVMEVRWGLIPDMSGSQTLRGLVR
                     RDVALELAWTGRVVPGEEALALGLVTRLADDPLAEALALADTIAGHSPDAVRGVKQLF
                     NRAWDCDSRTALALEAELQMKILGQANQMEAVRAGLSGSRPNFGPARE"
     gene            1255..2154
                     /locus_tag="PLS34_03175"
     CDS             1255..2154
                     /locus_tag="PLS34_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP86507.1"
                     /translation="MSETGVRPAARSAGGTGKRISLVLGSGGARGLAHIGVIQHLESL
                     GHEIVYISGCSMGALVGGIYAAGQLDSYAEWAKKLEKRDMFSLLDLAFGWTGLFKGDR
                     LMGVLKELVGDHVIEDLPIGFTAVATDLTEQREIWLNKGPLFDAIRASIAIPTVFTPH
                     VYLGRVLVDGGLLNPIPIAPALNQNADMIVAVNLNAHSRRSRRRASELAGRPTGEEAA
                     GQEEAAGEGGDPRVPGLIDVVTASLDLMQNSIARMKLASNTPDLVIDVPRDACLFYEF
                     YRAEEMIELGRECARGALAPRNA"
     gene            complement(2177..2689)
                     /locus_tag="PLS34_03180"
     CDS             complement(2177..2689)
                     /locus_tag="PLS34_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86508.1"
                     /translation="MQIRYLVIIVAALACAACASGQSSRTTSVDDDLAPKPVAPAPKR
                     VAVPEYLAFLDELTQSVEDEKPRVFNSRELREYRRVESELRASLAGVDNIDQLSHEQQ
                     IKVFNLHEELQAVFIGDPKYQVICRREQTVGTNFRRTSCMTVQEFRSYQDGGQSVLRG
                     AWPYMAPPAN"
     gene            complement(2812..3558)
                     /gene="gpmA"
                     /locus_tag="PLS34_03185"
     CDS             complement(2812..3558)
                     /gene="gpmA"
                     /locus_tag="PLS34_03185"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRP86509.1"
                     /translation="MGHKLILLRHGQSEWNLKNLFTGWVDVDLTEQGRAEAAEAARLL
                     QAEGLDFDLAFTSVLKRAIRTLWIVMDGMDRMWVPVTRAWELNERHYGALQGLDKAET
                     VRRHGEEQVLVWRRSYDVPPPPLAADDERHPRFEPRYAGIDPARLPAAESLKDTLARV
                     QPYWEAHIAPQILAGRQVMIAAHGNSLRALVKMLDGLSEEQILKVNIPTGIPLVYELD
                     DRLRSRGGRYLGDPEAARRAAEAVANQAKG"
     gene            complement(3595..4353)
                     /locus_tag="PLS34_03190"
     CDS             complement(3595..4353)
                     /locus_tag="PLS34_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRP86510.1"
                     /translation="MATEPLIAVFIDFENLALGVRDMKGDNKFKVELVIKRLLEKGRL
                     VFKRAYCDWSGYRDYTREFHSNGIEMIDIPQSRHSGKNSADIHMVVDALDLCYAKQHI
                     DIFALLSGDSDFSPLVSKLKENNKRVIGCGVKSSTSDLLIHGCDEFIYYDDLARVAAK
                     PRRTRGKSEPKDKKVEAMDKLVEIVESLDTDYDQLWGSMVKQTIRRVHPGFSESYYGY
                     NSFSDLLKDAESRGLIRLEYDEKRGNYLVSATAR"
     gene            complement(4385..9226)
                     /locus_tag="PLS34_03195"
     CDS             complement(4385..9226)
                     /locus_tag="PLS34_03195"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRP86511.1"
                     /translation="MHRRTADARSRFLEQVAAAATAGASDDLGLDVAAYIHQYYRNTS
                     LDDLKDREVENLAGAALSHLRLGLRREPDTPRILVFNPSRRTDGWSSDHTIVQVVNDD
                     MPFLVDSTSMVLNRHGSYIHLTVHPVLHTRRDERGRLVEVPTPEHAGPDARLESFMHL
                     EIDRETAPDVLQSVARELERVLGDVRAACQDWGAMRGRAREICAELETSPPPLDADIV
                     KESRELLEWMADDHFTFLGYREYELVRGDEADQLRALPDTALGILRRPPPPEVVGGTS
                     LAGREIRRQARSRDLLIITKANSRATVHRNSYLDYVGVKRFDAEGRVVGERRFLGLWT
                     SAAYSRSPREIPLLRDKLRRVIASSGLRPDSHGGKALVHILDTYPRDELFQSSLAELL
                     RISTGIYSLQERQRTKLFVRRDAFRRFFSCLVFVPRDRYNTQVRERIQEILLDAFRGV
                     SIESSVEMSESSLARVHFIVRTPQGTVPRIKVAAVERRIADGVRTWADHLRDELVERF
                     GEEDGLALLREYGKVFPAAYLEDVTPREATFDIERLEAIKAAPRRLRMSLYRPVDFGE
                     DRVRFKLFHGGSPMPLSEILPILENMGLRVISERPYHLRAGDGIDRDVWIQDFEMSVA
                     DGQAIKPGKVKDLFQDAFENIWIGRVENDGFNRLILKARLDWRQAALLRALCRYLLQT
                     GLPFSQAYMEQVLAEHALIAQMLVELFEGRFDPDLPEKRRERTVLTLNQAVSHALDQV
                     ASLDADRMLNAFRALILATLRSNYFQRDEHGRPKRYLSLKLDPHKIPDLPLPRPMFEI
                     FVYSPRVEGVHLRGGLVARGGLRWSDRREDFRTEVLGLMKAQAVKNVLIVPVGAKGGF
                     VPKRLPSGDRTALMKEVVDCYKTFINGLLDLTDNLVDGVVVPPHDVVRHDGDDSYLVV
                     AADKGTATFSDIANSVAAEHGYWLDDAFASGGSMGYDHKKMGITARGGWEAVKRHFRE
                     IGVDTQSDAFTAAGIGDMSGDVFGNGMLLSSCMRLRAAFNHQHIFLDPDPDPDTGFAE
                     RRRLFELPRSTWDDYDRSLISTGGGVWSRSDKLIQLSPQAREMLDLDQEGVTPDELIR
                     SILRMRVDLLWNGGIGTYVKASTETHPEVGDRANDAVRVNGCDLRCRVVGEGGNLGFT
                     QRGRIEFALRGGRINTDFVDNSAGVDCSDHEVNIKILISVANRHRALTVARREKLLVA
                     MTDEVAELVLRNNYLQTQAISIAESTAAERINEHAWFIRQLEKRVALNRTLEYLPDDE
                     EIELRRKAGKGLTRPELAVVMSYAKMALYGEMVRSNVPEDPYLAKELERYFPTPLRRR
                     YTDYLREHPLAREIIATMTTNSLVNRMGSVFAFRMQDETGSDIASVARAFSIAREAFE
                     IRAIWEAIEALDNKVSASVQHSMLRASQALLKQATRWLLDRREDLPDIAAAVRRLNPG
                     AMELIQCLPEVLLGSRLERYEGAAQLYADTGLPAPLARKVAALAPMHAALDIIALAEN
                     CAVRVRHAAEIYFRVGDALQLDWIADQIDGLRVEGMWQAKARGSLRDNLYGLHRALCA
                     NMLSAAPAEHDARAIVERWLTERADQAGHFSQTLEEMRDAGQADFATLSVALQEIRRL
                     VQE"
     gene            complement(9238..>9905)
                     /locus_tag="PLS34_03200"
     CDS             complement(9238..>9905)
                     /locus_tag="PLS34_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRP86512.1"
                     /translation="RQAPKLKLPPGKPEQLRGPRRAMAQSMSAARDHVSQCTIFDDAD
                     IHYWTRGQDITARLLRAIAAGCRAEPALNGFFDGGAMSRQLETRVDVAIAVDTPEGLI
                     VPVIRDVGQKSPQELRADLNAIKQATRERSVKPEDMRDFTFTLSNFGMMAGRYATPVV
                     VPPTIAILGAGGLRHDVVPVMGGIETHRRIPLSLTFDHRCATGGEACRFLAAVIEDLQ
                     RHD"
     assembly_gap    9906..9941
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9942..10449)
                     /locus_tag="PLS34_03205"
     CDS             complement(<9942..10449)
                     /locus_tag="PLS34_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRP86513.1"
                     /translation="MKTFNLPDLGEGLPEAEIVSWLVAEGDMVKVDQPMLSVETAKAV
                     VEVPAPYSGRIAKIHAPDGTILPTHSPLVDIDTSGAAAAKPAAPKSEPKAATAAAGKD
                     REDTGTVVGKMMTSDEVLEDADITGRGRRRGAGRVKAAPAVRALARQLGVDLERCRPT
                     GRHDRIIAD"
     gene            complement(10467..11444)
                     /locus_tag="PLS34_03210"
     CDS             complement(10467..11444)
                     /locus_tag="PLS34_03210"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRP86514.1"
                     /translation="MARITLIEAVTMALAWEMEHDDSVVVLGEDVGVNGGVFRATAGL
                     QQRFGPDRVMDTPLAESMIAGMSIGMAAQGIKPVAEIQFMGFIYPTVDMMINHAGRLR
                     NRTRGRLHVPMVLRAPFGGGIHAPEHHSESTEALFAHMPGIRVVIPSSPRRAYGLLLA
                     AIRDPDPVVFLEPKRIYRLQKQEVEDNGQALPLDTCYILREGDDVTIVTWGAMTFETL
                     KAAEELDKEGISAEVIDLATISPLDIDTILESVEKTGRLVIVHEAARTCGVGAEVAAG
                     VAEKGIWDLKAPIVRVTGYDTVMPLFRLEYEYLPSVARIIDGVKKTFED"
     gene            complement(11444..12526)
                     /gene="pdhA"
                     /locus_tag="PLS34_03215"
     CDS             complement(11444..12526)
                     /gene="pdhA"
                     /locus_tag="PLS34_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRP86515.1"
                     /translation="MKTIAEFRIPYRQVLDPEGRVVAELPDFARDPDELLRLYRLMTL
                     TRTFDTKAVNLQRTGKLGTYASCLGHEAAHVGIGAAMRPEDCLAPMYREYGAQFWRGV
                     RMEEVLLYWGGDERGNDFAGPKHDFPWCVPIATQCLHAAGAALAFKLREEPRCAVSVI
                     GDGGTSQGDFYEALNLAAVRDLPVVFVVVNNKWAISVPLALQTASKTLAQKAIAAGMP
                     GIQVDGNDVIAVRQTMEEALERARAGHGPMLVEAVTYRLSDHTTADDASRYREKGEVE
                     AAWKTEPMIRIRRYLIETGAWSEQQEEELKAECAQQVDAAVAAYLDTPVQPVEAMFDS
                     MFAELPEAMAEQRETARRFVNSGGHH"
     gene            12664..13503
                     /locus_tag="PLS34_03220"
     CDS             12664..13503
                     /locus_tag="PLS34_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRP86516.1"
                     /translation="MQRREMEAGIHRDLRDRLSYAGYLGLDRILSAQQPLSDPPHHDE
                     MLFIVQHQVTELWMKLIIHELEAAIASIRADALEPCFKVLARVKQVQQQMFHQWGVLE
                     TLTPAEYARFRHVFGQASGFQSVQFRLLEFLLGNKDAGYLAVFEHDAAASARLRAALE
                     APSIYDEFLRLLARRGLPVPAERVERDWSEPVDSHPGVVAIFKTIYEDTRQHWDAYDM
                     CEKLVDMEVNFQLWRFRHLKTVERIIGHKRGTGGSSGVKFLRAAIDIRLFPELIDVRT
                     AIE"
     gene            complement(13512..13973)
                     /locus_tag="PLS34_03225"
     CDS             complement(13512..13973)
                     /locus_tag="PLS34_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRP86517.1"
                     /translation="MNDDVLVLEEFLPYRLSVLSNRLSAAIAEAYSRRFGLSIPEWRV
                     MAVLALEPGLSAAEVADRTAMDKVAVSRAVGRLISTGRARREVSAGDRRRSMLELTAD
                     GRRIYRRIAPALQRYEAELLAVLDPPDRRQLEALLGRLESRAAALQPPRIE"
     gene            14073..>15096
                     /gene="hppD"
                     /locus_tag="PLS34_03230"
     CDS             14073..>15096
                     /gene="hppD"
                     /locus_tag="PLS34_03230"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRP86518.1"
                     /translation="MSEVQDNPLGIDGFEFVEFAAPDAALLHSLFRHMGFTAIGRHRE
                     RKVTLYRQGDINFLVNEEPDSFAADFAREHGPSACGFAIRVADAARARAWAIDRGAAA
                     LSNKPGTLAARSPVIEGIGGSALYLVDRYGKRGSIYDAEFEYFDGADRHPAGFGLTFI
                     DHLTHNVYQGNMDKWAGFYERIFNFREIRYFDIKGAQTGLVSRAMTAPDGMVRIPINE
                     ATDDKSQIAEYLEEYHGEGIQHIALFTDDIYATVEALRADGIEFLDVPDTYYEMVDGR
                     VPGHKEDLARMCENRILIDADKETKQQLLLQIFTQTCVGPIFFEIIQRKGNEGFGEGN
                     FQALFES"
BASE COUNT         2325 a         5013 c         5156 g         2566 t
ORIGIN      
        1 gccgatcgag cccgagttct acggcgagtt catcgacaag ctgggcctcg atcgcgagcg
       61 ttttgtgccg cacggcttcc ggccgggcag aacagacacc acgcgctggg ccgagctgaa
      121 ggccgagctg gccgcggtat tccgcacccg cagccgcgac gaatggtgcg cgctgctgga
      181 gggcagcgat gcctgtttcg cgccggtgct cgggctttcc gaggcgccgg cgcatcccca
      241 caatgcggcg cgcggcacgt tcatcgaagt cggcggcgtg gcgcagggcg cgccggcgcc
      301 gcgcttcagc cgcagtgcgc cggccatgcc ggaggccccg catcggcccg gcgcggacac
      361 gcgggccgtg ctcggcgagg cgggctatga tccaactgcg ctggccgggc tcgaggccgc
      421 cggcgtcatt ccagaggagc aggactgagc catgagcgag caacgcgtta ccatcgaacg
      481 gcgggaccac gtggcggtcg tcaccctggc gcggccggac aagcgcaatg cgctcgaccc
      541 ggcgatgttc gatgcgctgg acggcgcggc gcgcgaactg tcggccgacc acgagctgcg
      601 ggccgtggtg ctggcgggcg ccggcaagag cttttgcgcc ggcatcgacc tggcggcctt
      661 cgccgccggc gaggcggtga tccgcaagct gctggcgcgg cgcgacggcg aggctgcgaa
      721 tcttgcccag cgggtgggct ggggctggcg cgaatgcccc gtgccggtga tcgccgccct
      781 gcacggcgaa gtgttcggcg gcggtctgca ggtcgcgctc ggcgcggacc tcaggattgc
      841 gcgtccggat gcgcggttgt cggtcatgga agtgcgctgg ggcctgatcc ccgacatgtc
      901 cggcagccag acgctgcgcg ggctggtacg ccgcgacgtg gcgctggagc tggcctggac
      961 cgggcgcgtc gtgcccggcg aggaagcgct cgcgctgggc ctggtgacgc gcctggccga
     1021 cgacccgctg gccgaggcgc tggcgcttgc ggacacgatc gccggccact cgccggacgc
     1081 ggtgcggggc gtgaagcagc tgttcaaccg tgcctgggac tgcgattcgc gcaccgcgct
     1141 ggcgctggaa gccgagttgc agatgaagat cctcggccag gccaaccaga tggaagcggt
     1201 gcgcgccggg ctgtctggca gcaggccgaa tttcggcccg gcgagggagt gaccatgagc
     1261 gagaccgggg tgcggccggc cgcgcgcagc gccgggggca cgggcaaacg aatctcgctg
     1321 gtgctcggca gcggcggtgc ccgcggcctg gcgcacatcg gcgtcatcca gcatctcgag
     1381 tcgctgggtc acgagattgt ctacatttcc ggctgctcga tgggggcgct ggtgggcggg
     1441 atttacgcgg ccggccagct cgactcctat gccgagtggg cgaagaagct cgagaagcgc
     1501 gacatgttca gcctgctgga cctggcgttc ggctggaccg ggttgttcaa gggcgaccgc
     1561 ctgatgggag tcctcaagga gctggtcggc gaccacgtca tcgaggacct gccgatcggc
     1621 ttcaccgcgg tggcgaccga cctgaccgag cagcgcgaga tctggctgaa caaggggccg
     1681 ttgttcgacg ccatccgcgc gtccatcgcg atccccaccg tgttcacgcc ccacgtgtac
     1741 ctcggccgtg tgctcgtgga cggcggcttg ttgaacccga tcccgatcgc gccggcactg
     1801 aaccagaacg ccgacatgat cgtcgcggtg aacctgaacg cccattcccg cagatcacgc
     1861 cgccgcgcca gcgaactggc ggggcgcccg acgggggagg aggcggcggg acaggaagaa
     1921 gcggcagggg agggcggcga ccccagggtg cccggcctca tcgacgtggt gaccgcttcg
     1981 ctggacctga tgcagaattc catcgcgcgc atgaagctgg cgtcgaatac gccggacctc
     2041 gtcatcgacg tgccgcgcga cgcctgcctg ttctacgagt tctaccgcgc cgaggaaatg
     2101 atcgagctgg gccgggaatg cgcccggggg gcgctggccc cccggaacgc ctagccggcg
     2161 gcgcagcccg acgacatcag ttggcgggcg gcgccatgta gggccaggcg ccgcgcagca
     2221 ccgactggcc gccgtcctgg tagctgcgga attcctgcac cgtcatgcaa ctggtgcggc
     2281 ggaagttggt gcccaccgtc tgctcccggc ggcagatgac ctggtacttg ggatccccga
     2341 tgaacaccgc ctgcagttcc tcgtgcaggt tgaacacctt gatctgctgc tcgtggctca
     2401 gctggtcgat gttgtcgact cccgccaggc tggcacggag ctcgctctcg acgcgacgat
     2461 attcccggag ttcgcgcgag ttgaacacgc gcggcttctc gtcctcgacg gactgcgtca
     2521 gctcgtcgag gaacgccagg tactcgggca cggcgacgcg cttgggtgca ggcgcgaccg
     2581 gcttcggcgc caggtcgtca tcgacggagg tcgtgcggga actctgcccg gaggcgcagg
     2641 ccgcgcaggc gagcgcggcg acgatgatca caaggtaacg aatctgcatg aggcctgaat
     2701 ccaattaaag cgaaggaagt cgcgaagtat atcaattatt tgtacggccg ggtcgcctgt
     2761 cccgggcgcc gcatcagccg tggcggagac ctgccgcgga tggtgccttg atcagccttt
     2821 ggcctggttg gccaccgcct ccgcggcccg cctggcagcc tcgggatcgc cgaggtagcg
     2881 gccgcccctc gagcgcagcc ggtcgtcgag ctcgtagacc agcgggatgc cggtgggaat
     2941 gttgaccttg aggatctgct cctcgctgag cccgtcgagc atcttgacca gcgcacgcag
     3001 gctgttgccg tgcgccgcta tcatcacctg gcggccggcg aggatttgtg gcgcgatgtg
     3061 cgcttcccag tacggctgga ccctggcgag ggtgtccttg agcgactccg ctgccggcag
     3121 ccgcgccggg tcgatgccgg cgtaacgcgg ctcgaagcgg gggtggcgct cgtcgtcggc
     3181 ggccagcggc ggcggcggga cgtcgtagct ccgccgccag accagcacct gttcttcgcc
     3241 gtgacgacgc accgtctcgg ccttgtcgag accctgcaac gcgccgtagt gacgctcgtt
     3301 gagttcccag gcgcgggtca ccgggaccca catgcggtcc atcccgtcca tcacgatcca
     3361 cagggtgcga atggcgcgct tcaggaccga ggtaaaggcc aggtcgaagt ccagcccttc
     3421 ggcctgcagc agccgtgctg cttcggcggc ctcggcacgg ccctgctcgg tgagatcgac
     3481 gtcgacccac cccgtgaaca ggttcttgag attccattca ctctgcccgt ggcgcagcag
     3541 gatcagcttg tgtcccatcg ctcgtactcc cttgtgtccg tgtgccggcg cgtcctagcg
     3601 cgcggtggcg ctgaccaggt agttgccccg cttttcatcg tactccagcc gtatcaggcc
     3661 gcgggactcg gcgtccttga gcaggtcgga gaagctgtta tagccgtaat agctctcgct
     3721 gaacccggga tgtacgcggc ggatggtctg cttgaccatc gagccccaca gctggtcgta
     3781 atcggtgtcg agtgactcga cgatctccac cagcttgtcc atcgcttcga ctttcttgtc
     3841 tttcggctcg ctcttgccgc gcgtgcgcct gggtttggcc gccacccgcg cgagatcgtc
     3901 gtagtaaatg aactcgtcac agccatggat cagcagatcg gaggtggaac tcttcacgcc
     3961 gcagccgatg acccgcttgt tgttctcctt cagcttggag accagcggcg agaaatcact
     4021 gtcgccggac agcaaggcga agatgtcgat gtgctgcttg gcgtagcaga ggtcgagcgc
     4081 gtccaccacc atgtggatgt cggcgctgtt cttgccgctg tggcggctct gcgggatgtc
     4141 gatcatctcg atgccgttgg agtggaactc gcgggtgtag tcgcgatagc cgctccagtc
     4201 gcagtaggcg cgcttgaaca ccagccggcc tttttcgagc aggcgcttga tgaccagttc
     4261 gaccttgaac ttgttatctc ccttcatgtc ccgcacgcca agcgccaggt tctcgaaatc
     4321 gatgaaaact gcgatcagag gttcagtggc catgagaaac ttccttgcgt gatgcggccc
     4381 gcgttcattc ctgcacgagc cggcgtattt cctggagcgc gacggagagc gtggcgaagt
     4441 ctgcctggcc ggcgtcgcgc atttcctcca gggtctggct gaagtgtccg gcctggtcgg
     4501 cgcgttcggt gagccatcgc tcgactatag cccgggcatc atgctcggcc ggcgccgcac
     4561 tcagcatgtt cgcgcacagt gcccggtgca ggccgtagag attgtcccgc aggctgccgc
     4621 gggccttggc ctgccacatt ccctccacgc gcaggccgtc gatctggtcg gcgatccagt
     4681 ccagctgcag cgcatcgccc acccggaaat agatctcggc cgcgtggcgt acgcgcaccg
     4741 cgcagttctc ggccagcgcg atgatgtcga gtgccgcgtg catcggcgcg agcgccgcga
     4801 ctttgcgcgc cagcggcgcc ggcaggccgg tgtcggcata gagctgggcg gccccctcgt
     4861 agcgctccag ccggctgccc agcagcactt cgggcaggca ctgaatgagc tccattgcgc
     4921 cgggattgag ccttcgcacc gccgccgcga tgtcgggcag gtcttcgcgg cggtcgagca
     4981 gccagcgggt cgcctgcttg agcagggcct gggacgcgcg cagcatcgag tgctggaccg
     5041 aagcgctgac cttgttgtcc agcgcctcga tggcctccca gatggcacga atctcgaaag
     5101 cctcgcgcgc gatgctgaag gcacgggcca ccgaggcgat gtcggagccg gtttcgtcct
     5161 gcatgcggaa ggcgaacacg gagcccatgc gattgaccag gctgttggtg gtcatggtgg
     5221 cgatgatctc cctcgccagc gggtgctcgc gcaggtaatc cgtgtagcga cgccgcagcg
     5281 gcgtggggaa ataacgctcc agttccttcg ccaggtacgg atcctcggga acgttggaac
     5341 gcaccatttc cccgtacagc gccatcttgg cgtagctcat caccaccgcc agctccgggc
     5401 gtgtcaggcc cttgccggcc ttgcggcgca gctcgatctc ctcgtcgtcg ggcaggtact
     5461 cgagcgtgcg gttgagggcg acccgttttt ccagctgccg gatgaaccag gcgtgttcat
     5521 tgatccgctc ggccgccgtc gactccgcga tgctgatggc ctgggtctgc aggtagttgt
     5581 tgcgcaacac cagttcggca acctcgtcgg tcatcgccac cagcagtttt tcgcggcgcg
     5641 ccaccgtcag cgcccggtgc cggttggcga cactgatgag aatcttgatg ttgacctcgt
     5701 gatccgaaca gtccacgccg gctgagttgt cgacgaagtc ggtgttgatg cggccgccgc
     5761 gcagcgcgaa ctcgatccgg ccacgttgcg tgaatcccag gttgccgccc tcgccgacca
     5821 cgcggcagcg caggtcgcaa ccgttgacgc gcacggcgtc gttggcgcgg tcacccactt
     5881 cgggatgcgt ttcggtactt gccttgacgt aggtgccgat gccgccgttc cagagcaggt
     5941 ccacgcgcat gcgcaggatg ctgcggatga gctcgtccgg cgtgacgcct tcctggtcga
     6001 ggtccagcat ctcgcgcgcc tgcggcgaca gctggatgag cttgtcgctg cgcgaccata
     6061 cgccgccgcc ggtggaaata aggctgcggt cgtagtcgtc ccaggtggag cggggcagtt
     6121 cgaacagccg ccggcgctcg gcgaagccgg tgtccgggtc ggggtccggg tcgaggaaaa
     6181 tgtgctggtg gttgaaggcg gcgcgcaggc gcatgcagga cgagagcagc atgccgttgc
     6241 cgaacacgtc gccgctcatg tcgccgatgc cggcggccgt gaaggcgtcg ctttgcgtat
     6301 cgacgccgat ctcgcggaag tggcgcttga cggcttccca gccgccacgc gcggtgatgc
     6361 ccatcttctt gtggtcgtag cccatcgagc cgcccgaggc gaaggcgtcg tcgagccagt
     6421 agccgtgctc cgcggcgacc gaattcgcga tgtcggagaa agtggccgtg cccttgtcgg
     6481 ccgccacgac gagatagctg tcgtcgccgt cgtggcgcac cacgtcatgc ggcggcacca
     6541 ccacgccgtc caccaggttg tcggtcaggt cgagcaatcc gttgatgaag gtcttgtagc
     6601 agtccaccac ctccttcatc agcgccgttc ggtcgccgct cggcagccgc ttcggcacga
     6661 agcccccctt ggcgccgacc ggcacgatga ggacgttctt cacggcctgg gccttcatca
     6721 ggccgagcac ctcggtacgg aagtcctcgc gccggtcgga ccagcgcagc ccgccacggg
     6781 cgaccaggcc gccgcgcagg tggacgccct cgacgcgcgg cgaataaacg aagatctcga
     6841 acatcggccg cggcagcggc aggtcgggaa tcttgtgcgg gtccagcttc agcgaaagat
     6901 agcgcttggg acggccgtgc tcgtcgcgct ggaagtaatt gcttcgcagg gtcgccagga
     6961 tgagcgcacg aaaggcgttc aacatgcggt ccgcatcgag gctggccacc tggtcgaggg
     7021 cgtggctgac ggcctggttt agcgtcagca cggtgcgttc ccgccgcttc tccggcaggt
     7081 cgggatcaaa gcggccctcg aacaattcga ccagcatctg ggcgatcagc gcgtgttcgg
     7141 ccagcacctg ttccatgtag gcctgggaaa acggcaggcc ggtctgcagc aggtagcggc
     7201 acagcgcccg caacagcgcc gcctggcgcc agtcgagccg cgccttcagg atcaggcggt
     7261 tgaagccgtc gttctccacc cggccgatcc agatgttttc aaaggcgtcc tggaaaaggt
     7321 ccttgacctt gcccggcttg atggcctggc cgtcggccac gctcatctcg aaatcctgga
     7381 tccagacgtc gcgatcgatg ccgtcgccgg cgcgcaggtg gtagggccgc tcgctgatga
     7441 cgcgcaaacc catgttctcg agtatgggca ggatttccga cagcggcatc gggctgccgc
     7501 catggaacag cttgaagcgc acccgatcct cgccgaagtc cacgggacga tacaagctca
     7561 tgcgcagccg gcggggcgcg gccttgatgg cctcgaggcg ctcgatgtcg aaagtggctt
     7621 cccgcggcgt gacgtcctca aggtaagcgg ccgggaagac tttgccgtac tcccgcagca
     7681 acgccagccc gtcttcctcg ccgaagcgct cgacgagctc gtcgcgcagg tggtccgccc
     7741 aggtccgcac gccgtcggcg atgcgccgct ccaccgccgc caccttgatg cgcggcaccg
     7801 tgccctgcgg cgtgcgcacg atgaagtgca cgcgcgccag gctggattcg gacatctcga
     7861 ccgacgactc gatgcttacg ccgcgaaacg cgtccagcag tatctcctgg atgcgctcgc
     7921 ggacctgggt gttgtagcgg tcgcggggca cgaagaccag gcaggaaaaa aatcgccgga
     7981 aggcgtcgcg gcggacgaac agcttggtgc gctgccgctc ctgcagcgag tagatgccgg
     8041 tgctgatgcg cagcaactcg gccaggctgc tctggaacag ctcgtcgcgc gggtaggtgt
     8101 cgaggatgtg caccagcgcc ttgccgccgt ggctgtcggg gcgcagcccg gagctggcga
     8161 tgacgcgtcg cagcttgtcg cgcagcaacg ggatctcgcg cgggctgcgg ctgtaggccg
     8221 cggaagtcca gagcccgagg aagcggcgct cacctaccac gcgaccctcg gcgtcaaagc
     8281 gcttgacgcc gacgtaatca agatagctgt tgcgatggac ggtagcgcga gagttggcct
     8341 tggtgatgat caggagatcg cgggagcgcg cctggcggcg gatttcgcga cccgccaatg
     8401 acgttccacc gaccacctcc ggcggcggcg gcctgcgcag gatgccgagc gcggtgtccg
     8461 gcagggcgcg cagctggtcg gcctcgtcgc cgcggaccag ctcgtactcg cggtaaccga
     8521 ggaaagtgaa gtgatcgtcc gccatccact ccagcagctc gcggctttcc ttgacgatgt
     8581 ccgcatccag cggcggcggg ctcgtctcca gctcggcaca gatctcgcga gcccgcccgc
     8641 gcatcgcccc ccagtcctgg cacgccgccc gaacgtcgcc gagcacgcgc tcgagctcac
     8701 gggccacgga ttgcagcacg tcgggggcgg tctcgcggtc gatctcgaga tgcatgaagg
     8761 attcgagccg tgcgtcgggg ccggcatgct ccggggtggg cacctccacg agccggccgc
     8821 gctcgtcgcg ccgcgtgtgc aggaccgggt ggacggtcag gtggatgtag ctgccgtgcc
     8881 ggttgagcac catgctcgtc gagtcgacca ggaacggcat gtcgtcgttg acgacctgca
     8941 cgatggtgtg atccgagctc cagccgtcgg tgcgccgcga gggattgaac accaggattc
     9001 gcggcgtgtc cggctcgcgg cgcagtccga gccgcaggtg ggagagcgcg gcgcccgcca
     9061 ggttttccac ctcccggtcc ttcaggtcgt ccagggaggt gttgcgatag tactggtgga
     9121 tgtatgcggc gacgtccagc ccgaggtcgt cgctggcgcc ggccgtggcg gccgcggcca
     9181 cctgttccag gaagcgggag cgggcgtcag cggtgcgtcg atgcatgccg gagtccgtca
     9241 gtcatgcctt tgcaggtcct cgatcacggc agccaggaag cggcacgcct cgccgccggt
     9301 cgcacagcga tggtcgaagg tcagcgacag cggaatcctg cggtgcgtct cgatgccgcc
     9361 catcaccggc acaacgtcgt ggcgcagccc gccggcgccg agaatggcga tggtcggcgg
     9421 caccacgacc ggcgtggcgt agcgcccggc catcatgccg aaattggaga gcgtgaaggt
     9481 gaagtcccgc atgtcctccg gcttcacgct gcgctcgcga gtcgcttgct tgatggcgtt
     9541 cagatcggca cgcagctcct gcggggactt ctgtccgacg tcgcgaatca ccggcacgat
     9601 cagcccctcg ggcgtgtcca cggcgatcgc gacgtcgacc cgcgtctcga gctgccggct
     9661 catggcgccg ccgtcgaaga agccgttcag cgccggctcg gcgcggcagc cggccgcgat
     9721 ggcgcgcagc agccgcgcgg tgatgtcctg tccgcgcgtc cagtagtgga tgtcggcgtc
     9781 gtcgaagatc gtgcattgcg agacgtgatc gcgtgcggcg ctcatgctct gcgccatggc
     9841 gcgccgcgga ccccgcagct gctccggctt gcccggcggc agcttgagct tcggcgcctg
     9901 gcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtcggcga tgatgcggtc
     9961 atgccggccg gtggggcggc accgttcgag gtcgactccc agctgcctgg cgagcgcgcg
    10021 caccgccggg gcggccttga cgcgccccgc accgcgtctc cggccgcgtc cggtgatgtc
    10081 ggcgtcctcc agcacctcgt cgctggtcat catcttgccc accaccgtgc cggtgtcctc
    10141 gcgatccttg cccgcggccg cagttgcagc cttcggctcg gacttcggcg ccgcgggctt
    10201 cgccgcggcc gcgccgctgg tgtcgatatc caccagcggg gaatgggtcg gcaggatggt
    10261 gccgtccggg gcgtggatct tcgcgatgcg gccgctgtag ggcgccggca cctcgaccac
    10321 tgccttggcg gtctccaccg acagcatcgg ctggtccacc ttgaccatgt cgccctcggc
    10381 gaccagccag ctgacgattt ccgcttccgg cagtccctcg ccgaggtccg ggagattgaa
    10441 tgtcttcacg gtcatgaacg gcctccctag tcctcgaagg tcttcttcac gccgtcgatg
    10501 atgcgcgcga cgctgggcag gtactcgtac tcgagccgga acagcggcat caccgtgtcg
    10561 tagccggtca cgcgcacgat cggtgccttc aggtcccaga tgcccttctc cgcgaccccg
    10621 gcggcgactt cggcgccgac gccgcaggtg cgcgccgcct cgtgcacgat caccaggcgg
    10681 ccggttttct cgaccgactc caggatggtg tcaatgtcca gcgggctgat cgtggccagg
    10741 tcgatgacct ccgcgctgat gccctccttg tcgagttcct cggccgcctt gagcgtctcg
    10801 aaggtcatcg cgccccaggt gacgatggtg acgtcgtcgc cttcgcgcag gatatagcag
    10861 gtgtccagcg gcaatgcctg gccgttgtcc tccacctcct gcttctgcag ccggtagata
    10921 cgcttgggct cgagaaacac caccgggtcg ggatcgcgga tggccgccag cagcaggccg
    10981 taggcgcgcc gcggcgagga ggggatcacc acccggatgc cgggcatgtg ggcgaacagc
    11041 gcctcggtgc tttcggaatg gtgctccggg gcgtggatgc cgccgccgaa gggcgcgcgc
    11101 agcaccatcg gcacgtgcag gcggccgcgc gtacgattcc gcagccggcc cgcatggttg
    11161 atcatcatgt cgacggtggg atagatgaag cccatgaact ggatctcggc gaccggcttg
    11221 atgccctggg cggccatgcc gatggacatg ccggcaatca tcgactccgc cagcggcgtg
    11281 tccatcaccc ggtccggccc gaagcgctgc tgcaggccgg cagtggcgcg gaatacgccg
    11341 ccgttgaccc cgacgtcctc gcccagcacc acgacgctgt cgtcgtgctc catctcccag
    11401 gccagcgcca tggtcacggc ctcgatgagc gtgatgcgcg ccatcagtgg tggcctcccg
    11461 agttgacgaa gcgtcgcgcc gtttcgcgct gctccgccat ggcctccggc agctccgcga
    11521 acatggaatc gaacatggcc tcgaccggtt gcacgggcgt gtccaggtac gccgccacgg
    11581 ccgcgtcgac ctgctgcgcg cactcggcct tgagctcttc ctcctgctgc tcgctccacg
    11641 cgcccgtctc gatcaggtag cgacggatgc ggatcatcgg ctccgtcttc caggcggcct
    11701 cgacctcgcc cttctcgcga tagcggctgg cgtcgtccgc cgtggtgtgg tcggacaagc
    11761 ggtaggtcac ggcctcgacc agcatcgggc cgtggccggc cctggcgcgc tccagcgcct
    11821 cttccatggt ctggcgcacc gcgatgacgt cgttgccgtc gacctggata ccgggcatgc
    11881 ccgcggcgat cgctttctgc gccagcgtct tggacgccgt ctgcaaggcg aggggcacgc
    11941 tgatggccca cttgttattg accacgacga acacgacggg caggtcccgg accgcagcca
    12001 ggttgagagc ttcgtaaaag tcgccctgcg aggtgccgcc atcgccgatc acgctgacgg
    12061 cgcaacgcgg ctcctcgcgg agcttgaacg ccagcgccgc cccggcggca tgcaggcact
    12121 gggtggcgat cgggacgcac caggggaaat cgtgtttcgg cccggcgaag tcgttgccgc
    12181 gctcgtcgcc gccccagtag agcagcacct cttccatgcg cacgccgcgc cagaactgcg
    12241 cgccgtattc gcggtacatc ggcgcgaggc agtcctcggg gcgcatggcc gcgccgatcc
    12301 cgacatgcgc ggcctcgtga ccgaggcagg aggcgtaggt gcccagcttg ccggtacgct
    12361 gcaggttgac ggccttcgtg tcgaaggtcc gcgtcagcgt catgagccgg tacagtcgca
    12421 gcaattcatc cggatccctg gcgaaatcgg gcagctcggc cacgacccgg ccttcaggat
    12481 cgagtacctg ccggtacggg atccggaact cggcaatcgt cttcaccagg cgttcctcct
    12541 gatactctgg tcgcgggccg gaagagcggt ccgcaaatag cgcggcatta tacacttcgg
    12601 cgacggtcgc cgacctgccg cggggtgccc gcggatgttg cgacgccgca gcaggagacg
    12661 tcgatgcaga gaagggaaat ggaggccgga atccaccgcg acctgcggga ccggctgagt
    12721 tacgccgggt acctcgggct ggaccgcatc ctgtccgcgc agcagccgtt gtccgatccg
    12781 ccgcaccacg acgagatgct gttcatcgtg cagcaccagg tgaccgagtt gtggatgaaa
    12841 ttgatcatcc acgaactgga agcggccatt gcgtcgatcc gcgcggacgc gctggagccc
    12901 tgcttcaagg ttctggcccg ggtcaagcag gtacagcagc agatgttcca ccagtggggc
    12961 gtgctggaga ctctcacgcc cgccgagtac gcgcgcttcc ggcacgtgtt cggccaggcc
    13021 tcgggcttcc agtcggtgca gttccgcctg ctcgagttcc tgctcggcaa caaggacgcc
    13081 ggctatctcg cggtgttcga gcacgacgcc gctgcatcgg cgcgcttgcg cgcggcgctg
    13141 gaggcgccct cgatctacga cgagttcctg cgcctgctgg cgcgccgtgg cctgcccgtg
    13201 ccggccgagc gggtcgagcg cgactggtcg gagcccgtcg actcccaccc cggcgtggtg
    13261 gcgatattca agaccatcta cgaggacacc cggcagcact gggacgccta cgacatgtgc
    13321 gagaagctgg tcgacatgga ggtcaatttc cagctgtggc gcttccggca tctcaagacg
    13381 gtcgagcgca tcatcggcca caagcgcggc acgggcgggt cgtccggcgt caagttcctg
    13441 cgcgcggcca tcgacatccg gctgttcccg gaactgatcg acgtgcgcac cgccatcgag
    13501 tagcgccgcg ctcattcgat gcgcggtggc tgcagcgcgg cggcgcgcga ctcgaggcgc
    13561 cccagcaatg cctcgagctg ccggcggtcg ggcgggtcca ggactgccag caactcggcc
    13621 tcgtagcgtt gcagcgccgg cgcgatgcgg cgatagattc gtctgccgtc ggcggtgagc
    13681 tccagcatgc tgcggcggcg gtcgccggcc gagacttcgc gccgtgcgcg gccggtcgag
    13741 atcagccggc cgacggcgcg gctgaccgcg accttgtcca tggccgtgcg gtcggcgact
    13801 tcggccgcgg acaggccggg ctccagcgcc agcacggcca tcacgcgcca ttccggaatg
    13861 gacagcccga agcgcctgga gtaggcctcc gcgatcgcgg cgctcagccg attggacaac
    13921 accgacaggc gatagggcag gaattcttcc aggaccagca cgtcgtcgtt catggtgcat
    13981 cgcagcttgt tggcctgaca cccacaggac tataatggtt tcacttgtaa ctaatcaact
    14041 cttcctgtac atgcattccg ccggagattg ccatgagcga agttcaggac aaccccctgg
    14101 gcattgacgg tttcgagttc gtcgaattcg ccgcgccgga cgcggccttg ctgcacagcc
    14161 tgttccggca tatgggtttc accgccatcg gcaggcaccg tgagcgcaag gtcacgctgt
    14221 atcgtcaggg cgacatcaat ttcctggtca atgaagagcc ggacagcttc gcggccgact
    14281 tcgcgcgcga acacggtccg agcgcctgcg gcttcgcgat ccgcgtggcc gatgccgcgc
    14341 gcgcgcgggc ctgggcgatc gaccgcggcg ccgctgcgtt gagcaacaag cccggcaccc
    14401 tggccgcccg tagtccggtg atcgagggca ttggcggcag cgcgctttac ctggtggatc
    14461 gctacggcaa gcgcggctcc atctacgatg ccgaattcga gtacttcgac ggcgcggacc
    14521 gccacccggc gggtttcggc ctgactttca tcgatcacct gacccacaac gtctaccagg
    14581 gcaacatgga caagtgggcg ggtttctacg agcgcatctt caacttccgc gagatccgct
    14641 acttcgacat caagggcgcc cagaccgggc tggtgtcgcg cgccatgacc gcgccggacg
    14701 gcatggtgcg gatccccatc aacgaggcga ccgacgacaa gtcgcagatc gccgagtacc
    14761 tggaggagta ccacggtgag ggcatccagc acatcgcgct gttcaccgac gacatctatg
    14821 cgacggtcga agcgctgcgc gccgacggca tcgagttcct cgacgtgccg gacacctact
    14881 acgagatggt cgacggccgt gtgcccgggc acaaggagga cctggcgcgg atgtgcgaga
    14941 accgcatcct gatcgacgcc gacaaggaaa cgaagcagca gctgctgttg cagatcttca
    15001 cgcagacctg cgtcgggccg atctttttcg agatcatcca gcgcaagggc aacgagggct
    15061 tcggcgaggg caacttccag gcgctgttcg aatcga
//
LOCUS       DAOMPG010000036        15073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1712_length_15073_cov_5.114796, whole genome shotgun sequence.
ACCESSION   DAOMPG010000036 DAOMPG010000000
VERSION     DAOMPG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_15073_cov_5.114796"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLS34_03235"
     CDS             <1..307
                     /locus_tag="PLS34_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRP86519.1"
                     /translation="LFFPQHFLGLAGMPRRIPDYNVAFSDWNLVSSIGAFGMFVTPFM
                     MAFILLHSLRRGEKASARVWEGARGLEWTVPSPAPHHTFARPPVIRPGDLAHEDTLH"
     gene            348..476
                     /locus_tag="PLS34_03240"
     CDS             348..476
                     /locus_tag="PLS34_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008577456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86520.1"
                     /translation="MNPPIQDNDTQARRRGVRRTVVVTTVLVVLIYAAFVAGLIGR"
     gene            473..1036
                     /locus_tag="PLS34_03245"
     CDS             473..1036
                     /locus_tag="PLS34_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRP86521.1"
                     /translation="MSAPDASTRVLKRGLWVAALAFGLCFSLVPLYRIACEQLFGIKL
                     GGPGGAVAAEIDTSRWVTVEFDAGVNSGLPWAFRPAQAEVKVRPGEPTDVLYYARNES
                     ALALVGQAVPSVAPSRASAYFNKTECFCFTEQMLAAGEERPMPVRFVVDPDLPTDIHR
                     LTLSYTFYKNEPATQRLAGELAAHAAP"
     gene            1064..1939
                     /locus_tag="PLS34_03250"
     CDS             1064..1939
                     /locus_tag="PLS34_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRP86522.1"
                     /translation="MAHATHDADLYFVPKQSAWPLIGSIGMFTLAVGTANWFNGHLWG
                     KAVFFLGIAIMIGMFIGWFGAVIRESVAGSYNRQVDVSFRMGMIWFIFSEVMFFAAFF
                     GALYYARNFSVPWIGGEGHGVMTNALLWSGFDPAWPSNGPAQVGGSFQTMSAWGLPLI
                     NTVLLLTSGVTLTIAHHALKAGNRSKVLLWLGLTVLLGATFLFFQVEEYIHAYTELNL
                     TLGSGIYGSTFFMLTGFHGAHVTLGAIMLTVIWFRVLKGHFTADDHFGFEAVAWYWHF
                     VDVVWLLLFLFVYVL"
     gene            complement(2058..2267)
                     /locus_tag="PLS34_03255"
     CDS             complement(2058..2267)
                     /locus_tag="PLS34_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRP86523.1"
                     /translation="MWPKVIIFTFLVLILYNLGAGLYYMIVDKGRTKRTVNALSWRVG
                     LSVALVVLLILGIWTGMISPHGVGG"
     gene            2367..3086
                     /locus_tag="PLS34_03260"
     CDS             2367..3086
                     /locus_tag="PLS34_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRP86524.1"
                     /translation="MNAGARVAWAALALIVAGGFASLGFWQAGRADEKTRMLAAREAA
                     QATGPALLADALSADSPSLPMRVEGEVVIRPAPLLLLDNQQRDGRVGVRAYVPADAGR
                     GGVVLMELGWLPLAADRTLPAVEVPAGRRRIQGLLLPWPGQGLRLADNPWPEGADTIL
                     LAFLERDDIAAHLDVQVWNGILQPDPADPADPLGYAREPGLLPDPMPPERHRGYAVQW
                     WGLSLTVIITYLLLALRRKSR"
     gene            3083..3652
                     /locus_tag="PLS34_03265"
     CDS             3083..3652
                     /locus_tag="PLS34_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86525.1"
                     /translation="MNQTLPFDPVRRRRNRIMLVLLVLCFVLPLMVAVALQQSGWRPE
                     HTANRGTLLQPPVALADLALRHADGSEYRYAPQEARWQIAVVPPADCGAACGELIDSL
                     AKVWQLQGRRADRLQVLWFGTVPEQADGFRALLPMQVDPALNARLPGLATSGAPSAYL
                     IDPRAFLVMHYAPGFDPGDLRADVAKLLK"
     gene            3649..4812
                     /locus_tag="PLS34_03270"
     CDS             3649..4812
                     /locus_tag="PLS34_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRP86526.1"
                     /translation="MSLMTPHRNFHRLAWIATLLALFVIVFGAFVRLSNAGLSCPDWP
                     TCYGKATWPVHAHEIDRANDAFDRPVESHKAWREQVHRHLAATLGMLVFALAFLGSRR
                     LRGGKAVVIGASLLVALAIPLYMRGEYGLSSALAVAGETILLGWALRPGSVASSRGDY
                     SRISALLLAVIVFQALLGMWTVTWLLKPVVVMGHLIGGMTTMALLAWMACRASPQASL
                     VLPAAWRLRPLLIGAIAVVAIQIALGGWVSANYAALACGIDYPTCLGQWWPEHDFREA
                     LVLWRGIGTDYEGGVLDGPARVAIQMMHRLFALVALGWLVWLGRRLRAVPEISGWGTA
                     VLILIGLQFGLGILNVISGLPLKIAVAHNAVAALLLFVLVVLLARLRRPEAAA"
     gene            4812..5684
                     /gene="cyoE"
                     /locus_tag="PLS34_03275"
     CDS             4812..5684
                     /gene="cyoE"
                     /locus_tag="PLS34_03275"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRP86527.1"
                     /translation="MGPKLRQYWTLTKPGVVALIAFTAVIGILLAVPGLPPLVPALAG
                     LVGIWLAASSAAAINHLIDQRIDVVMARTAHRPLPSGQLRPRQVLAFACALAALSMTV
                     LVLWTNVLTAVLTFASLIGYAIVYTAWLKRATPQNIVIGGLAGAAPPLLGWVAVTGEV
                     HPHALLLLLIVFVWTPPHFWALAIFRRDDYARALIPMLPVTHGVVYTRWHVLFYTLLL
                     TVATMLPWLTGMSGPFYLGGALVLDAVFIYYAIRLLNPPSERFAMAVFGYSIVYLMLL
                     FAFLLIDHWLPPPA"
     gene            complement(5660..6655)
                     /locus_tag="PLS34_03280"
     CDS             complement(5660..6655)
                     /locus_tag="PLS34_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRP86528.1"
                     /translation="MNLRFLFDRFSLALIATVVLASLWPVAGVAATWFSHLTTAGIAL
                     LFFLHGARLPREAVVAGLRHWPLHATVLAATFALFPLLGLAVQPLAGALLTPELYLGV
                     LFLCALPSTVQSSIAFTSIARGNIAAAVVAASVSNLVGVFLTPLLAALLLATHGAMGS
                     PWRAIIDILLLLLAPFVLGHLLRPWLARWVQRRARLLSLTDRGTILLVVYGAFSHAVI
                     EGLWAQVPGRTLLGLVAVACALLALVMGLIWSATRRLGFSRADRITILFCGSKKSLAT
                     GVPMANILFAGQATLGMIVLPVMVFHQIQLMVCAVLAQRFAQQVPADDQAGGGSQ"
     gene            6841..8199
                     /locus_tag="PLS34_03285"
     CDS             6841..8199
                     /locus_tag="PLS34_03285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP86529.1"
                     /translation="MPTLTFLGGAGTVTGSKHLVSDGDARILVDCGLFQGLKNLREQN
                     WEPLPVRARDVDAVVLTHAHLDHSGYLPRLVRDGFSGRIHATPATCEVAALILRDSAH
                     LQEKDAEYANRKGFSRHRPALPLYRSSDAERAIGRFAPTPPHREFRLPGEARCLLRRA
                     GHILGAATVQMDIGGRRLVFSGDLGRYDDPVMFDPEPVPEADYVVIESTYGDRLHERI
                     DPVQALGAVIERTVRRGGTVVIPAFAVGRTQSLLWYLWRLRQDGRLNNTPVYLDSPMA
                     TSATELMREWPDEHRLDPADYEAACVAVTCVRDVEESKALSANPYPKVIISASGMATG
                     GRVLHHIAAFGRDHANTLLFSGYQASGTRGRALLAGRREVKMFGQWIPIAAEVTDLPM
                     LSAHADSDELMRWLSGFRRRPERVFIVHGEPDASEALRVRIGRELGWNACVPREGQRF
                     SL"
     gene            complement(8276..11188)
                     /locus_tag="PLS34_03290"
     CDS             complement(8276..11188)
                     /locus_tag="PLS34_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRP86530.1"
                     /translation="MKPAARPSVNPTLLILAATLLPAGGAAGAVPDAVTPPAAAPRVM
                     LDAGAANEAVSGFIVYYRDDAAPGEENAKAAVDTRKAVDQDLARVKAALSIEAKRDRR
                     LATGGHLLSVPKALGKSAALGFMREMATNPQIDSIEPNVRHWPTAVPNDPHYSSQWGL
                     REAVGGLNIEPAWDHTVGSGVVIAVIDTGRTAHPDLDAKTVPGYDFISNAAEARDGSG
                     RDSDPSDMGTWNSAGECGAGEPAKDSSWHGTHVAGIAAALTHNGLGIAGAAPGAAIQH
                     VRVLGRCGGTTADIAEGIIWASGGQVSGVPTNPTPARVLNLSLGGSGECGTTYQRAID
                     SARSRRSVLVVAAGNDSMAAALARPANCSGVITVAANNREGKKSDYSNYGLAVNVAAP
                     GGEGSGDGMILSTVNSGRTTPLSPTYAGMNGTSMAAPYVAGVVALMLERNLALTPDQV
                     AGILRDTARPFPSYCLGGCGTGIVDAAAAVRRVRGGPFYAFPASVAIYGNGAGTVTSL
                     PLGITCGTSCASRFDANTTITLRATPEEGYEFTGWAGACQGTSRTCELAMTRGHAVFA
                     TFKIPVLAMSNGSVRTGLAASGSRKLMFSIDVPAGATNLAFEMSGGSGDADLYVRRDA
                     EPTTDGETFDCRPWKIGNSETCSFEAPVAGRYFAMISADGSFSGVQLRTRYTAGPNGG
                     SSLARNAPVRNVSIPADGARYYRFTVPQGATDLSIKMQGSSGDADLYVRRGGVPTFDT
                     FDCRPYLVGSNETCATATPAAGTYYVMLHAEAAVSGLTLTANYTPFVPTRQLTIGWAG
                     LGGGSVAIRRAATGETLATCGAFPCTVAVPENISFDLIPTAAFGSSFAEWQGVCDSRP
                     GANYCRIRLAAARSLTARFVMSRPNSPALTIERQGSGNGTVLVKRLSTGETLGTCTQY
                     PCRLGPPDATHELIATPGAGSRFLGWMPSQCDSIVGGHCRVRISRPMAMTARFSPQ"
     gene            11561..11677
                     /locus_tag="PLS34_03295"
                     /pseudo
     CDS             11561..11677
                     /locus_tag="PLS34_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11746..12408)
                     /locus_tag="PLS34_03300"
     CDS             complement(11746..12408)
                     /locus_tag="PLS34_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86531.1"
                     /translation="MADLEAKAAAGSVIARRRLAMIYGACFGYSLDPDAHFAMIDAFA
                     AHSGGHRQPFESLKSRTDQRCLGANGGKPISIDEIRHLMESAAQGGDLVAQIDVETHS
                     IEPIKAEKMEELIAQVISSRDPDAMLAMSSLARKGIDGSLDMPYSMLVGDPISEAAWA
                     IAGCQAGANCGPGSIVLDSICTGTGACGYTSYEMFLRQHMVPPGEVSRLNEYLLLILN
                     GP"
     gene            14091..14786
                     /locus_tag="PLS34_03305"
     CDS             14091..14786
                     /locus_tag="PLS34_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP86532.1"
                     /translation="MRAIIAVALAGAMLFWAALAQAACVDNVVLVHGNAGSPSDFDNT
                     YTELRARGYAASQIFRPGWGNRLCPACNDHSGTEETPVRNALVDAIAGSCTGRIDVIG
                     HSMGVTLAARQIDVWGLKSYVDSFVGIAGAYRGLWSCGSYPWNGWTSTCGAQGLSIGS
                     PLLNGLAGKKPGARAYSIKSNIDQIVCSTGVCTVGGVHSSTIPGENASYTFATGHFGL
                     LTGTAVTQANLIQ"
     gene            complement(14813..>15073)
                     /locus_tag="PLS34_03310"
     CDS             complement(14813..>15073)
                     /locus_tag="PLS34_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HRP86533.1"
                     /translation="HLAQGDLRFEPETALASGADGLDALRTLARGAPGHLAPGGWLLL
                     EHGLDQGEAVRGLLRDAGFHRIETLRDLEGRERVTLGCRAPD"
BASE COUNT         2355 a         5111 c         5021 g         2586 t
ORIGIN      
        1 gctgttcttc ccgcagcact tcctcggcct ggccggcatg ccgcgccgca tcccggacta
       61 caacgtggca ttctccgact ggaacctggt cagctcgatc ggtgccttcg gcatgttcgt
      121 cacgccgttc atgatggcct tcatcctgct gcactcgctg cgccgcggcg agaaggccag
      181 cgcgcgcgtc tgggaaggtg cccgcggcct ggagtggacg gtaccttcgc cggcaccgca
      241 ccataccttc gcccgcccgc cggtgatccg ccccggagac ctggcgcacg aggacactct
      301 gcactgaggc ggcgctgcgg cgaagccccg tgccggacac catggccatg aatccaccga
      361 tccaggacaa cgacacccag gcgcgccgcc gcggcgtgcg ccgcacggtg gtggtgacca
      421 ccgtgctggt cgtcctgatc tacgcggcgt tcgttgccgg gctgatcggc cgatgagcgc
      481 gccggacgcc agcacccggg tgctcaagcg cgggctgtgg gtcgcggccc ttgcctttgg
      541 cctgtgtttc tcgctggtgc cgctgtatcg catcgcctgc gaacagctct tcgggatcaa
      601 gctcggcggg ccgggcggcg cggtggcggc ggaaatcgac acctcgcgct gggtcacggt
      661 ggagttcgat gccggggtga actcgggcct gccctgggcc tttcggccgg cccaggccga
      721 ggtgaaggtg cgcccgggcg aaccgaccga cgtgctctac tacgcccgca acgagagcgc
      781 gctggcgctg gtcgggcagg cggtgccctc ggtggccccg agccgcgcct cggcctattt
      841 caacaagacc gaatgcttct gcttcaccga gcagatgctg gcggccgggg aagagcggcc
      901 gatgccggtg cgcttcgtgg ttgatccgga cctgccgacc gatatccacc gcctcacgct
      961 ttcctatacc ttctacaaga acgagccggc cacgcagcgc ctcgccggtg aactcgccgc
     1021 gcacgcggcc ccctgaccga ccagccaaac ctcgggaatc gccatggccc acgctaccca
     1081 cgacgcagat ctctacttcg ttcccaagca gagcgcctgg ccgctgatcg gctccatcgg
     1141 catgttcacg ctcgccgtgg gaaccgccaa ctggttcaac ggccacctct ggggcaaggc
     1201 cgtcttcttc ctcggcattg cgatcatgat cggcatgttc atcggctggt tcggcgcggt
     1261 catccgcgag tcggtggccg gttcctacaa ccgccaggtc gacgtgtcct tccgcatggg
     1321 catgatctgg ttcatctttt ccgaagtgat gttcttcgcc gcgttcttcg gcgcgctcta
     1381 ctacgcgcgc aacttctcgg tgccgtggat cggcggcgag ggccacggtg tgatgaccaa
     1441 cgcgctcctg tggagcggct tcgatccggc ctggccgagc aacggcccgg cgcaggtggg
     1501 cggcagcttc cagacgatgt cggcctgggg cctgccgctg atcaacacgg tgctgctgct
     1561 cacttccggc gtgaccctga ccatcgccca ccacgcgctc aaggccggca accgcagcaa
     1621 ggtgctgctg tggctggggc tgaccgtcct gctgggtgcg accttcctgt tcttccaggt
     1681 cgaggagtac atccacgcct ataccgagct gaacctgacg ctcggctcgg gcatctacgg
     1741 ttcgacgttc ttcatgctta ccggcttcca cggtgcacac gtcacgctgg gcgcgatcat
     1801 gctgacggtc atctggttcc gcgtactcaa ggggcatttc accgccgacg accatttcgg
     1861 tttcgaagcg gtggcctggt actggcactt cgtcgacgtg gtgtggctgc tgctgttcct
     1921 gttcgtgtac gtgctctgag gccgggattc gggaatcggg agctgggaat cgtcgagggc
     1981 gtcggggtgg ctgaaccttt cactccggca tcaacctgct cctgaccctt cccgattctc
     2041 cattcccggc gtttgaatca gccgcccacg ccgtgcggcg agatcatccc ggtccagatg
     2101 cccaggatca gcaggaccac gagcgccacg ctgaggccga cgcgccagct cagggcgttg
     2161 acggtgcgct tggttcgacc cttgtccacg atcatgtagt acagccccgc gccgaggttg
     2221 tagaggatca ggaccaggaa cgtgaagatg atgacttttg gccacatggt gatatccgac
     2281 tctgcgtgcg tggggtggcg gccaggtgcc ctgggcggga acttcgaccg gacaaacgat
     2341 tttcgcacgg cggcgggcgc gcgcgcgtga acgccggtgc gcgcgtcgcc tgggccgcgc
     2401 tggccctgat cgtggccggc ggctttgcct cgctgggatt ctggcaggcg ggccgcgccg
     2461 acgaaaagac gcgaatgctg gcggcgcgcg aagcggcgca agcgaccggc cccgcactgc
     2521 tcgccgatgc cttgagcgcc gattcgcctt cgttgccgat gcgggtcgag ggcgaggtcg
     2581 tgatccgccc ggcaccgctg ctcctgctcg acaaccagca gcgcgacggg cgcgtgggcg
     2641 tgcgcgccta tgtccctgcc gatgccggga ggggtggcgt ggtgctgatg gaactgggct
     2701 ggctgccgct ggcggccgac cgcacgctgc cggcggtgga agtccccgcc ggccgccgcc
     2761 ggatccaggg gctgctgctg ccgtggccgg ggcagggcct gcgtctggcc gacaatccct
     2821 ggccggaagg cgcggacacg atcctgctcg ccttcctgga gcgcgatgac atcgccgcgc
     2881 atcttgacgt ccaggtgtgg aacggcatcc tccagcccga tccggccgat ccggccgatc
     2941 cgctcggcta cgcacgcgag cccgggctcc tgcccgaccc gatgccgccc gaacggcacc
     3001 gcggatacgc ggtgcagtgg tggggcctgt cgctgactgt catcatcacc tatttgctgc
     3061 tggccctgcg ccggaaatcc cgatgaacca gaccctgccc ttcgatcccg tccgccgtcg
     3121 ccgcaaccgc atcatgctgg tgctcctggt gctctgcttc gtgctgcccc tgatggtcgc
     3181 cgtggccctg cagcaaagcg gctggcgccc ggagcacacg gccaaccgcg gcacgctcct
     3241 gcagccgccg gtggcgctgg ccgacctcgc gctgcgccat gccgacggca gcgagtaccg
     3301 ctatgccccg caggaagcgc gctggcagat cgccgtggtg ccgccggccg actgcggcgc
     3361 ggcgtgcggc gagctgatcg acagcctcgc caaggtctgg cagctgcagg gtcggcgcgc
     3421 cgatcggctc caggtgctgt ggttcggcac cgtgccggaa caggccgacg gtttccgcgc
     3481 gctgctgccg atgcaggtcg atccggcgct caatgcgcgc ctgcccgggc tggccacgag
     3541 cggggcaccg tccgcctacc tgatcgatcc gcgcgccttc ctggtgatgc actacgcgcc
     3601 tggcttcgat ccgggcgacc tgcgcgcgga cgtggccaag ctgctgaaat gagcctcatg
     3661 acaccgcacc gcaacttcca ccgcctggcg tggatcgcga ccctgcttgc gctgttcgtg
     3721 atcgtcttcg gtgccttcgt gcggctttcc aacgccggat tgtcctgccc ggactggccg
     3781 acctgctacg gcaaggcgac ctggccggtg catgcgcacg agatcgaccg ggccaacgac
     3841 gccttcgacc gcccggtcga atcgcacaag gcctggcgcg agcaggtgca ccgccacctg
     3901 gccgccacgc tcgggatgct ggtgttcgcg ctggcgttcc tcggctcgcg ccgcctgcgc
     3961 ggcggcaagg ccgtcgtgat cggggccagc ctgctggtgg cgctggcgat cccgctgtac
     4021 atgcgcggcg agtacgggct gtcttccgcg ctggccgtgg ccggagagac gatccttctg
     4081 ggctgggcgc tgcgcccggg cagcgtcgct tcgtcgcgcg gcgactattc ccgcatttcc
     4141 gcgctgctgc tggcggtgat cgtgttccag gcgctgctgg gcatgtggac ggtgacctgg
     4201 ctgctcaagc cggtggtggt gatggggcac ctcatcggtg gcatgaccac gatggcgctg
     4261 ctggcctgga tggcctgccg cgcctcgccg caggcctcgc tggttcttcc ggcggcgtgg
     4321 cggctgcgcc cgctgctgat cggtgcgatc gccgtcgtcg ccatccagat cgcgctgggc
     4381 ggctgggtgt cggccaacta tgcggcgctg gcctgcggca tcgattaccc gacctgcctg
     4441 ggccagtggt ggcccgagca cgatttccgc gaagccctgg tgctgtggcg cggcatcggc
     4501 accgactacg agggcggcgt gctggacggg ccggcgcggg tggcgatcca gatgatgcac
     4561 cgcctgttcg cgctggtggc gctgggctgg ctggtgtggc tggggcgcag gctgcgcgcg
     4621 gtgccggaaa tttccggctg gggcacggcc gtgctgatcc tgatcggcct gcagttcggc
     4681 ctcggcatcc tcaacgtcat ttccggcctg ccgctgaaga tcgcggtcgc gcacaacgcg
     4741 gtggcggcgc tgctgctgtt cgtgctggtg gtgttgcttg ctcggctgcg ccgacccgag
     4801 gcggccgcct gatgggcccc aagctgcgcc agtactggac gctgaccaag cccggcgtgg
     4861 tggcgctgat cgcattcact gcggtcatcg gcatcctgct ggcggtgccg ggcctgccgc
     4921 cgctggtgcc ggcgctcgcg ggtctggtcg gcatctggct ggcggcttcg tcggccgcgg
     4981 ccatcaatca tctgatcgac cagcgtatcg acgtggtgat ggcgcgcacc gcgcaccggc
     5041 cgctgcccag cggccagctg cggccgcgcc aggtgctcgc gttcgcctgc gcgctggcgg
     5101 cgctgtcgat gacggtgctg gtgctgtgga ccaacgtgct cacggcggtg ctgaccttcg
     5161 cctcgctgat cggctacgcc atcgtctaca ccgcctggct caagcgcgcc acgccgcaga
     5221 acatcgtgat cggcggcctg gcgggcgccg cgccgccgct gctgggctgg gtcgcggtca
     5281 ccggcgaggt gcatccgcac gcgctgttgc tgcttctgat cgtgttcgtc tggaccccgc
     5341 cgcacttctg ggcgctggcg atctttcgcc gcgacgacta cgcccgcgcc ctgatcccga
     5401 tgctgccggt cacccacggc gtggtctaca cgcgctggca cgtgctgttc tacaccctgc
     5461 tgctgaccgt ggcgacgatg ctgccctggc tcaccggcat gagcggaccg ttctacctgg
     5521 gcggggcgct ggtgctcgat gcggtcttca tctactacgc gatccgcctg ctgaatccgc
     5581 cgtcggagcg tttcgcgatg gcggtgttcg gctattcgat cgtctacctg atgctgcttt
     5641 tcgccttcct gctgatcgat cactggctgc cgccgccggc ctgatcatcc gccggcacct
     5701 gctgcgcgaa gcgctgcgcc agcaccgcgc agaccatcag ctggatctgg tggaacacca
     5761 tcaccggcag cacgatcatg cccagcgtgg cctggccggc gaacaggatg ttggccatcg
     5821 gcacgccggt ggcgaggctc ttcttcgagc cgcagaagag aatcgtgatg cggtcggcgc
     5881 ggctgaaccc gagccggcgc gtcgcgctcc agatcagccc catcaccagc gccagcagcg
     5941 cgcaggctac cgccacgagg cccagcagcg tgcgccccgg cacctgcgcc cacaggcctt
     6001 cgatcaccgc gtggctgaag gcgccgtaga ccaccagcag gatcgtgccg cgatcggtca
     6061 gcgacagcag ccgcgcgcgc cgctgcaccc agcgcgcgag ccagggccgc agcaggtgtc
     6121 ccagcacgaa cggagccagc agcaggagca ggatgtcgat gatcgcgcgc cagggcgaac
     6181 ccatcgcccc gtgcgtggcc agcagcagcg ccgccagcag cggggtgagg aagaccccga
     6241 ccaggttcga gaccgaggcc gcgaccacgg cggcggcaat gttgccgcgc gcgatcgagg
     6301 tgaaggcgat ggacgactgc acggtcgagg gcagggcgca caggaacagc acgcccaggt
     6361 acagctccgg cgtcagcagc gcgccggcca gcggctgcac ggcaaggccc agcagcggga
     6421 acagcgcgaa ggtggcggcc agcaccgtcg catggagcgg ccagtggcgc aggcccgcca
     6481 ccaccgcctc gcgcggcagc cgcgcgccgt gcaggaagaa aagcagcgcg atgcccgccg
     6541 tggtgaggtg cgaaaaccag gtggccgcca cgcccgcgac cggccacagg ctcgccagca
     6601 ccacggtggc gatcagcgcc agcgaaaacc ggtcgaacag gaagcgcagg ttcatcccgg
     6661 cattgtgccc aagtgccgca gcgtcgggcg cggttgccgc gccgcgcagg ctagaatcgc
     6721 gcaacacgaa tcggcggagg acgtgatgga tcgaacgagc gaagggcgcg ccgccagccg
     6781 cgcgcgcagg ggcttgccgg acagcaggtc cacgtcgcaa tccatcgccg cggcccgccc
     6841 atgccgacgc tgacctttct cggcggcgcc ggcaccgtca ccggctccaa gcacctggtc
     6901 agcgacggcg atgcccgcat cctggtcgat tgcggcctgt tccaggggct caagaacctg
     6961 cgcgagcaga actgggagcc gctgccggtc cgcgcccgcg acgtcgatgc cgtggtgctc
     7021 acccacgcgc acctggacca ttcgggctac ctgccgcggc tggtgcgcga cgggttttcc
     7081 ggcaggatcc acgccacccc ggcgacctgc gaggtggccg ccctgatcct gcgcgacagc
     7141 gcccacctgc aggaaaagga cgccgagtac gccaaccgca aggggttttc caggcacagg
     7201 ccggcgctgc cgctgtatcg ctccagcgac gccgagcgcg ccatcggccg tttcgcgccg
     7261 accccgccgc accgcgagtt ccgcctgccg ggcgaggcga ggtgcctgct gcggcgcgcc
     7321 ggccacatcc tcggcgccgc caccgtgcag atggacatcg gcggccggcg cctggtgttc
     7381 tcgggcgatc tcgggcgcta cgacgacccg gtgatgttcg atcccgagcc ggtgcccgag
     7441 gccgactacg tcgtcatcga atcgacctac ggcgatcgcc tgcacgaacg catcgatccg
     7501 gtgcaggcgc tgggcgcggt gatcgagcgc acggtgcggc gcggcggcac cgtggtgatc
     7561 ccggccttcg ccgtgggccg cacccagtcg ctcctgtggt acctgtggcg cctgcgccag
     7621 gacgggcggc tgaacaacac gccggtctac ctcgacagcc ccatggccac cagcgccacc
     7681 gagctgatgc gcgagtggcc cgacgagcat cgcctcgatc cggccgatta cgaagccgcc
     7741 tgtgtcgcgg tcacctgcgt gcgcgacgtg gaggagtcca aggcgctgtc ggccaacccg
     7801 tatcccaagg tcatcatttc cgccagcggc atggcgaccg gcgggcgcgt gctgcaccac
     7861 atcgcggcct tcgggcgcga ccacgccaat accctgcttt tctccggcta ccaggccagc
     7921 ggcacgcgcg gacgcgcgct cctggcgggc cggcgggagg tcaagatgtt cggccagtgg
     7981 attccgatcg ccgccgaggt gaccgacctg cccatgcttt cggcccatgc cgacagcgac
     8041 gagttgatgc gctggctgtc cggcttccgc cgccgtcccg aacgggtctt catcgtgcac
     8101 ggcgagccgg acgcctccga agccctgcgc gtgcgcatcg gcagggagct gggctggaac
     8161 gcctgcgtgc cgcgcgaggg acagcgcttt tccctgtagc cacgaaggtg tgcgtaccgg
     8221 acccgaagac cgaaccgggg tgccgcggca ccccgatgtc cggtgccgga tggcgctatt
     8281 ggggagaaaa ccgcgccgtc atcgccatgg gccgggaaat gcgcacgcgg cagtggccgc
     8341 cgacgatgct gtcgcactgg ctgggcatcc atccgaggaa gcgcgaaccg gcgccgggcg
     8401 tggcgatcaa ctcgtgggtg gcgtcgggcg gcccgaggcg gcagggatac tgcgtgcagg
     8461 tgcccagcgt ctccccggtg gacagacgct tcaccagcac ggtgccgttg cccgaaccct
     8521 ggcgttcgat cgtcagcgcc gggctgttgg gccgcgacat cacgaagcgt gcggtcaggc
     8581 tgcgtgcggc cgcgagcctg atgcggcagt agttcgcgcc gggccggctg tcgcagacgc
     8641 cttgccactc ggcgaagctg gaaccgaacg ccgcggtggg gatcaggtca aaggagatgt
     8701 tctcgggtac ggcgaccgtg caggggaagg cgccgcaggt ggccagggtc tccccggtgg
     8761 ccgcgcgccg tatggccacg ctgccgccgc ccaggcccgc ccagcctatg gtgagctgcc
     8821 gggtcggcac gaagggggtg tagttcgcgg tgagggtcag cccgctcacc gccgcctcgg
     8881 cgtgcagcat gacgtagtag gtgccggcgg ccggcgtggc cgtggcgcag gtctcattgc
     8941 tgccgaccag gtagggacgg cagtcgaagg tgtcgaaggt cggcacgccg ccgcggcgca
     9001 cgtacaggtc cgcatcgccg ctgctgccct gcatcttgat gctgaggtcg gtggcgccct
     9061 ggggcacggt gaaccggtag tagcgcgcgc cgtccgccgg gatcgacaca ttgcgcaccg
     9121 gcgcgttgcg ggccagggag ctgccgccgt tcgggccggc ggtgtagcgg gtccgcagtt
     9181 gcaccccgct gaagctgccg tcggcggaaa tcatcgcgaa ataccgcccg gccacgggcg
     9241 cctcgaagct gcaggtttcg ctgttgccga ttttccaggg tcggcagtcg aaggtttcgc
     9301 cgtccgtcgt cggctccgca tcgcggcgca cgtagaggtc cgcatcgccg ctgccgccgc
     9361 tcatctcgaa cgccaggttc gtggcgcccg ccggaacgtc gatggagaac atcagcttcc
     9421 tgctgccgga cgccgccagg ccggtgcgca ccgagccgtt gctcatcgcc agcaccggga
     9481 tcttgaaggt ggcgaagacg gcgtgccccc gcgtcatggc cagctcgcag gtgcgcgacg
     9541 ttccctggca ggcgccggcc cagccggtga attcgtaacc ctcttccgga gtggcgcgca
     9601 gggtgatcgt ggtgttcgca tcgaaccggc ttgcgcacga agttccgcag gtgatgccca
     9661 ggggcaggct cgtcaccgta cccgcaccgt tgccgtagat cgccacgctc gccgggaacg
     9721 cgtagaaggg cccgccgcgc acgcgccgca ccgcggcggc cgcatcgacg atgccggtgc
     9781 cgcagccgcc caggcagtag ctggggaagg ggcgcgcggt gtcgcgcagg atgccggcca
     9841 cctggtcggg cgtgagcgcg aggttgcgtt ccagcatgag ggccaccacg ccggccacgt
     9901 agggcgcggc catggaggtg ccgttcatgc cggcgtaggt gggcgagagc ggcgtggtcc
     9961 ggcccgaatt gaccgtggaa aggatcatcc cgtcgcccga gccctctccg cccggcgcgg
    10021 cgacgttcac cgccagcccg tagttcgagt agtcggactt ctttccctcg cggttgttgg
    10081 ccgccacggt gatgacgccg ctgcagttgg ccgggcgcgc cagcgcggct gccatcgaat
    10141 cgttgccggc cgcgaccacc agcaccgagc ggcgcgagcg cgcgctgtcg atcgcgcgct
    10201 gataggtggt gccgcattcg ccgctgccgc ccaggctcag gttgagcacg cgcgccgggg
    10261 tggggttggt gggtacgccg ctgacctgcc cgccgctggc ccagatgatg ccttcggcga
    10321 tgtcggccgt ggtgccgccg caccggccca gcacccgtac gtgctggatc gccgcgcccg
    10381 gcgcggcccc ggcgatgcca agcccgttgt gggtcagggc cgcggcgatg ccggccacgt
    10441 gggtgccgtg ccagctggaa tccttcgccg gctcgccggc gccgcactcg ccggcgctgt
    10501 tccaggttcc catgtcggat ggatcgctgt cgcgcccgct gccgtcgcgc gcttccgccg
    10561 cgttgctgat gaagtcgtag ccgggcacgg tcttggcatc gagatccggg tgcgcggtgc
    10621 ggccggtgtc gatgaccgcg atcaccaccc cgctgcccac ggtgtggtcc caggccggct
    10681 cgatgttgag cccgccgacc gcctcgcgca gcccccactg gctggaatag tggggatcgt
    10741 tcggcacggc ggtgggccag tgccggacgt tgggctcgat ggagtcgatc tgcggattgg
    10801 tggccatctc gcgcatgaag cctagcgccg cgctctttcc cagcgccttg ggaacgctca
    10861 gcagatggcc gccggtggcc agccgccgat cgcgcttggc ctcgatggac agcgccgcct
    10921 tgacgcgcgc cagatcctga tccaccgcct tgcgcgtatc caccgcggcc ttggcgttct
    10981 cctccccggg cgcggcgtcg tcgcggtagt agacgatgaa tccgctgacc gcctcgttcg
    11041 ccgctcccgc gtcgagcatc acgcgcggcg cggccgcggg cggcgtgacc gcatcgggca
    11101 cggcccccgc cgcgccaccg gcgggcagga gggtggcggc gagaatcagc agggtggggt
    11161 tcacgctggg gcgggctgcg ggtttcatgc ggcacctcgg tctgagtgaa ggggcgggcg
    11221 catcgcgtcg cgtccggcag ggagagaagg cgcaagaccg cgccgaaccg gctcacccat
    11281 gggatcagta cgcaatcggc gacggatcgt gtcacgccgc cgccggcgtg cgcagcggcg
    11341 ctcgggcgtg ccgcacacgc ggctcctcca cccccaatgc tccggactgt ctagtctcgg
    11401 gactcagccg gcagttccct ctcccccatg caaatggggg agagggtcgg ggtgaggagg
    11461 aactgtccgt gattaaagca accgaacctg cggcgctttc ccttcatccc aagaaacctc
    11521 tgaacgagct gctgcagaaa atctggcagc caatactgtt cgcgcgaaca aggcgcacgt
    11581 ctttgactac atcgatgtct actacaatcg aatccggctc cattcgactc tgggctatcg
    11641 aagccctgag cagttcgagc catcccgtgt cgcttaaacg ggtgtccgag aaacaggggc
    11701 aggatcagat cacgagagag cccaaaagtc ggagcggttc ggggtctatg ggccattcaa
    11761 gatcaacagg agatattcgt tcagcctgga tacctcgcca ggtggaacca tgtgttgacg
    11821 aaggaacatc tcataggatg tatagccaca tgctccagtc cctgtacata ttgaatccag
    11881 gacaatcgag cctggcccgc agtttgcgcc cgcctggcag ccagcaatgg cccacgcggc
    11941 ctcggaaatc gggtctccaa ccagcatcga atagggcatg tccaacgatc catcaatacc
    12001 ttttctagcc aaggaggaca tggcgagcat ggcatctgga tcacgactcg aaatgacctg
    12061 ggcgatcaat tcttccatct tctcggcctt tattggctca atggaatgcg tctccacgtc
    12121 tatctgcgca accaagtcgc caccctgtgc agcggactcc atcagatggc gtatctcatc
    12181 gattgaaatg ggcttgcctc catttgctcc gaggcaacgt tgatcagtac gggatttcag
    12241 gctttcaaac ggttgcctat gacctcctga atgtgcagca aacgcatcta tcatggcgaa
    12301 atgggcatct ggatcaagac tatacccgaa gcatgcgcca taaatcatgg cgagcctccg
    12361 ccgggctatt acagagccgg ctgcggcttt tgcttccagg tctgccacct catgtctcag
    12421 gtcttggctt tcccccaagg aataatcggg cgcgctacca gtcgcagttg attggtggct
    12481 atcgtgtatg gtgcttaccg gagtctgact ggcctccctt gtgccagata tggcagcgct
    12541 ctcaggctcc gtctcagaga agaatccgaa taaattcccc catgccatta gcgacagaac
    12601 cacgagcacg agcacgaaaa gcgccacaat ctttaaattt ttatttcttg atacgggcat
    12661 cgtcttctcc ttaagacaag tcagacatct cgacgacttc aagttcctaa gtaattacgc
    12721 tataatacgt attaattttc aatcaactgt cgatcatagc tttgcacttc cccatacacc
    12781 aatcttgaag tctctagaca caaacaacga cgactccagt ggattcacct cttgaggaac
    12841 aaccggagtg aaccagcaaa atcaattggt tacgcaggca tggacgaccg ggatctggct
    12901 gttcctctgc gaacgcctgc cccgtgtgca gggttgagaa gctgattagc gtcgaatctc
    12961 agtggtgcct cggatcatct tcccacgaaa tcaatagatt gcatgtttag ggccgtaatg
    13021 aaaataatcc acaggcaggg caggattaac ctgttgccct gcccgggact aaactcacct
    13081 catcccaaca cccagcgtca tggatgctct aattccccaa cttcaaccgt accacaccca
    13141 ctgccataac accccggagg aggcaggggt ggattccagg gaggaagacc gccgccgccg
    13201 ccgccaccac cgccaccact cggtggtggc tgctgtgtat tagctattgg ctgcacacaa
    13261 tagcttccta tcaagcagac gacagtaaat ttttcttttg agccatcctg atatgtaact
    13321 gtgacaacag tgccaactgg gactgacaag gcaccatgtg ttgttcgata catccctact
    13381 agccctagcc cggctgctat gcgaagatcg ttcatatcac ctataccaat cccctgagcc
    13441 ggggcaactt gattatacgg tgaattcctt gcgtcaatgg atataccttg cgaatatgcc
    13501 ggagcagaag cgaagagcaa aaaaagaact gcgaaaacta ttctaaccat ctacttatcc
    13561 ccaatcaatt aattaattga atcccctgtg gagcttaatc tacccagcct ctaacgagct
    13621 gtcaacacta caagttatcc tgaccagctc cggccgggga aaagcgggcg aaaatgctgc
    13681 gcagcggcag tcgggacgaa agaatcgggg ccatggggaa atacctccgc aagcggcaaa
    13741 taaactcccc cgttcccgca gcctagccca tgcgcgccgg ctcgtccatt acccggatgg
    13801 ggtggggggg cttgatcggc ctcgaatctc agctcgatgc cccggaattc agctgccgtc
    13861 attctggcgc ggcgctctac aaaaactgaa aggcttgcca aaccggaatg acgccagttt
    13921 tttgaggtgc ccagaggttc ctcatttcgg tcccgtaccc cggttcgggt gatcggacgg
    13981 caggccgccg cctcgtacct tcgtacagat tccttccgtg acgttccgcg ctagggtcgt
    14041 tgcgtgaccg ggcgattcgc cccggcgcga tccttgggag gggaaacacc atgcgtgcaa
    14101 tcattgccgt cgcgctggct ggcgcgatgc tgttctgggc ggcgctggcg caggcggcct
    14161 gcgtcgacaa cgtggtgctg gtgcacggca acgccggcag cccgtcggat ttcgacaaca
    14221 cctacaccga actgcgcgca cggggctatg cggcgtcgca gatcttccgg ccgggctggg
    14281 gcaaccggct ctgcccggcc tgcaacgacc acagcgggac cgaggaaacc ccggtcagga
    14341 acgccctggt cgatgcgatc gccggctcgt gcaccggcag gatcgacgtg atcgggcatt
    14401 cgatgggcgt gaccctggcg gcgcgccaga tcgacgtctg gggcctgaag tcctacgtcg
    14461 acagcttcgt gggcatcgcc ggggcctatc gcggcctgtg gtcgtgcggc agctacccgt
    14521 ggaacgggtg gacctcgacc tgcggcgcgc aggggctgtc catcggcagc ccgctgctca
    14581 acgggctggc gggcaagaag ccgggcgcac gcgcctattc gatcaagtcc aacatcgacc
    14641 agatcgtgtg ctcgaccggc gtgtgcaccg tcggcggcgt gcactcctcg accattccgg
    14701 gcgagaacgc cagctacacg ttcgccaccg gccacttcgg cctcctgacc ggcaccgcgg
    14761 tcacccaggc caacctgatc cagtagccga tccctggcgt tggagccgcg cgtcaatccg
    14821 gcgcgcggca gccgagcgtg acgcgctcgc ggccttccag gtcgcgcagc gtctcgatcc
    14881 ggtggaatcc ggcgtcgcgc agcaggccgc gcacggcttc gccctggtcc agcccgtgtt
    14941 cgagcagcag ccagccgccc ggcgcgaggt ggcccggcgc gccgcgcgca agggtgcgga
    15001 gggcgtcgag gccgtcggcg ccgctggcca gggccgtctc cggctcgaag cgcaggtcgc
    15061 cctgggcgag gtg
//
LOCUS       DAOMPG010000037        15002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1721_length_15002_cov_4.277848, whole genome shotgun sequence.
ACCESSION   DAOMPG010000037 DAOMPG010000000
VERSION     DAOMPG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15002
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_15002_cov_4.277848"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PLS34_03315"
     CDS             complement(<1..1428)
                     /locus_tag="PLS34_03315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRP86534.1"
                     /translation="MAGKDVAPNPFFTASTLPYSVPPFDQITDDHYLPAFERGMEEEL
                     AEIELIANNPEPPTFENTIVAMERTGPILERVSRVFFNLNSAHTNAQMQEVQKTVSPL
                     LAAHQDAIYLNGALFARVEALHARRDELGLDPESKFLLERYHTDFVRAGARLSEADKE
                     KLRELNRELASLATQFAQNVLQETINSAVVVDTAEELDGLTESQIRGMANAARERGLE
                     GKYLVTLQNTSGQPPLSSLTNRALRQRIHEASVNRGARDNENDNRPVIARTAALRAER
                     AKLLGYPNHAAFVLENATAGTTDAVNSMLTGLAPAAVANARREAADIQALIDAQGGDF
                     QLQAWDWDFYAEQVKQARYDLDPAQLKPYFELESVLNNGVFYAANQVFGLTFKPRPDL
                     PLYHPDARAWEVFEADGTPLGIFIGDFYARDSKRGGAWMNAYVPQNGLEGTKPVVANH
                     QNIPKPPEGEPTLMTFTEVTTMFHEF"
     gene            1826..2962
                     /locus_tag="PLS34_03320"
                     /pseudo
     CDS             1826..2962
                     /locus_tag="PLS34_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            3423..3749
                     /locus_tag="PLS34_03325"
     CDS             3423..3749
                     /locus_tag="PLS34_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP86535.1"
                     /translation="MLNVADGNFPSEFATGKPALIEEERRLLYVAMTRAKSSLHLVAP
                     LKYWVPQQPRHGDRHVYGARSRFIDDAMMACLEKRFHGAAEAGVSGSPRPAEVVDLGA
                     RMRTMW"
     gene            3760..4512
                     /locus_tag="PLS34_03330"
     CDS             3760..4512
                     /locus_tag="PLS34_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019992415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRP86536.1"
                     /translation="MSVLGIDIGGSGIKGAPVNLVEGRLATARHKLPTPQPATPEAVT
                     ASVVELAGQFELRGSVGVAVPAVVKHGVTQTAANIDPAWVGCDIGRLLHDALGRPVVV
                     LNDADAAGLAEMRYGAGRGRRGTVVMLTLGTGIGSALFVDGVLWPDTEFGHLDVRGKA
                     AEHRAAAQLRKAEGLSWEKWAARVSEVLVAIERLLWPDLYIIGGGVSRKQEKFLPLLE
                     CRTPVVPAALGNDAGIIGAALAAIGDTPHQSH"
     gene            4509..5333
                     /locus_tag="PLS34_03335"
     CDS             4509..5333
                     /locus_tag="PLS34_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP86537.1"
                     /translation="MKSLGTILLFVALPYAAMVAFLWLRQESMLFLPHMPSRDIVATP
                     ADIGLRYEPLQLPTADGETLAAWFVPAPEARAVLLFFHGNAGNISHRLDSLRLFHDLG
                     LSVLIIDYRGYGQSTGRPTEPGTYEDARAAWRHLVEERGIPEQRIVLFGRSLGGAIAT
                     WLAAHTAPRALIVESAFRSVPDMAAEIYWFLPVRLLARLHYPVESLLPQVTAPVLIIH
                     SRDDEIIPFAHGEVLHAAAPEPKKLLELQGDHNTGFLQSRSHYSAGIDAFLSSHGL"
     gene            complement(5366..7486)
                     /locus_tag="PLS34_03340"
     CDS             complement(5366..7486)
                     /locus_tag="PLS34_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRP86538.1"
                     /translation="MQQKITVALLCATLAACAATSPRPAAPGYPETRRVNHVDEYFGT
                     RVADPYRWLEQLDSAEVDAWVAAQNEYSAKVVEALPDYERIRARLEVLWTTERMSVPR
                     VHDGRYFFEYGDGRATLSKLMVADSLDGEWRMLLDPEALSADGTTLLARWNVSPDGQY
                     VAWAATVGGSDWTRWKVRRTDTGEDLPHGLEGTKFTSAAWLKDASGFFYARYPKNAAG
                     EWDDLQQAEVWLHRIGTPQAEDTHVFSITDHPTRTPFPQASSDGRHLVLGIGQDSRAN
                     GIYLLPLDEPGAEVIRLVDSWDGRAGYLGTVGDTVYISTSIGAPRGRVVAIDLARPAQ
                     ADWREVVPESALPIEGVSLIGAQLIVRYMEDAKSRVELFALDGTPQGALALPGIGKVS
                     GFTGSPESAETFYQFESFDNPGTIYRYRSADGEVGRFHQKATGLDTSVLETRQVFYES
                     KDGTRVPMFIVHRKGIELDGRNPTMLYGYGGFRVSMQPDWDLRLLGWLDLGGVLAIAN
                     LRGGGEYGADWHLAGTRERKQTTFDDFIAAAEWLIAQRWTSPDKLAIYGRSNGGLLIG
                     AVQLQRPELFAAAGPAVGVLDMLRYHTPSANARAWAGDFGLSENETDFRAQLAYSPVH
                     NVRPGTCYPATIIHTGKFDDRVVPWHSYKFAAALQHAQGCDRPVLLRTETRAGHASGG
                     AKPRYLLIDEFAELYAFFAWALGM"
     gene            complement(7491..8648)
                     /locus_tag="PLS34_03345"
     CDS             complement(7491..8648)
                     /locus_tag="PLS34_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP86539.1"
                     /translation="MDFKFTEEQQMIQEAARDFAQREIAPIAAEFDASGQFPEQTLRK
                     AGELGFMGVEVPEEYGGAGLDAIGFALMMEEIAAADAAHSTVISVHNSLYCNGILQYG
                     TEEQKQKYVTPVASGEVFAAYGLTEPQSGSDAANMRTRAVRKGDHYVINGKKSWITSG
                     PVAKYVMLFAMTDPSAGPKGVSAFMIDTEKKGFACGKTEPKLGIRASATCEIELADYV
                     CPVEDRLANEGDGFKIAMSILDAGRIGIGAQAVGIARAAYEASLEYVRERHAFGAPIG
                     SFQMIQARIADMKMRLEAARLLTLKAAWNKKVAAAAGLRNTTEASCAKLFASEAAMFI
                     THAAVQIHGGMGYSKEMPIERYFRDAKITEIYEGTSEIQRMVIARQETGLR"
     gene            complement(8711..9214)
                     /gene="greB"
                     /locus_tag="PLS34_03350"
     CDS             complement(8711..9214)
                     /gene="greB"
                     /locus_tag="PLS34_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HRP86540.1"
                     /translation="MSRYRPPQPKSSPYITPEGAARLREELDRLWRVDRPEVVRALAD
                     AAAEGDRSENAEYIYRKKQLGGLDRRIRHLRRRLGALKVVDRSPADTTQVFFGAWVTV
                     EDETGAESRFRIVGPDEFDHAPGYISMDAPLARALMKRSPGDEVTLELAAGRKTWLLL
                     EVRYEPA"
     gene            9308..9742
                     /locus_tag="PLS34_03355"
     CDS             9308..9742
                     /locus_tag="PLS34_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP86541.1"
                     /translation="MKNLLIVPLLLAWCGVATAADEVEWFSKGPGGEDRIVMHYFYSD
                     TCPVCTEAKPFIEALREVAWLEVRAFEVSANRDNAIRYRDTAAAIGGQARSVPAYIAC
                     GAMLTGYNGPEPTWERLSVIFKRCMPAGGEPSAAETPPAAGD"
     gene            9743..10426
                     /locus_tag="PLS34_03360"
     CDS             9743..10426
                     /locus_tag="PLS34_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRP86542.1"
                     /translation="MHFLLLTILLLGLVFGPGLWVKHVLAKYATPADRYRGSGAQLAR
                     FLLDRRGLESVAVEQTEQGDHYDPLAKAVRLSPDNFRGRSLTAITVAAHEVGHAVQDH
                     TGYPPLKLRHRLVTSMRHVERLGAGLLMVSPFIGMLTRALIVGIVMFVGGLLTLGTAT
                     LVHLVTLPTEIDASFGRALPMLEEEEILIEDDLPHAERLLRAAAFTYVAASLMSLLNV
                     ARWWAIIRR"
     gene            10465..11517
                     /locus_tag="PLS34_03365"
     CDS             10465..11517
                     /locus_tag="PLS34_03365"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRP86543.1"
                     /translation="MSTVKAYAAQSPTSAVEPFAIKRRPPRPDDVVIEIDYCGICHSD
                     IHTAHNDWGFTSYPVVPGHEIVGHVTAVGNEVKNFKPGDRVGVGCLVDSCRRCGSCES
                     GLEQYCERGWVGTYNGKDLHDGSPTYGGYSERIVVSERFVLRIPDTLDAAAAAPLLCA
                     GITTWSPLKHFGVGKGHRIGVVGMGGLGHMGVKFARALGAEVTVFTRTVAKVDEAKKQ
                     GAHHVIVSTNDKQMAAAAASFDFILDTVPVPHDLNPYVNALKRDGTLILVGLLEPIEP
                     ALQAFPIVLGRRRIAGSLIGGLPETQEMLDFCAEHGIVCDIEMLDIRNINEAWERTQR
                     SDVRYRFVIDMATLRD"
     gene            11517..12218
                     /locus_tag="PLS34_03370"
     CDS             11517..12218
                     /locus_tag="PLS34_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86544.1"
                     /translation="MRRMALRLAVAAVVLLALSVPAFWPQYLSRPGESDAYTHFHAGL
                     GLAWLLLLIGQPLLVRARRLDLHRRIGRVGWLLGAAFAVSGVLVAHRGVARMDEAELA
                     RDGFFLYMPLAQSALFAVALALGIAWRSEPATHARYMACTALPLLDPLLARLLHFYFP
                     PLPAGFLYQLPAFFLTVGVLAVLSGSMPRSSRGRGGFRAFAAGTIVTLLLFFVTPYSA
                     AWATFVAWFRALPVT"
     gene            12241..12927
                     /locus_tag="PLS34_03375"
     CDS             12241..12927
                     /locus_tag="PLS34_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRP86545.1"
                     /translation="MIEPDDQEASTTSSRLRHGSLLLLGLAVAYSPSYINVALGLAGV
                     EWEFTKGPPSVLLWNWLAVAVLLVFILKVEGRGLGSIALRRPSGRDIQWAVMFWAVST
                     VASGMLHQFLPPPPSTGMATVLQLSLLTILALVFTTAVTEEVLFRGYSIERLSELTGY
                     RPLAVAVSFGLFVLPHVAFFGVQWLLYHGLGVVLLYVLYVWRRNLWACMIMHLLGNAL
                     LLIPALGLAD"
     gene            12957..13280
                     /locus_tag="PLS34_03380"
     CDS             12957..13280
                     /locus_tag="PLS34_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86546.1"
                     /translation="MIGNRFEYGPLARGIYIALAWMTPLVAIVWWELLLWHLLLLAFL
                     GLGLRPLLEKTGIHALYQHVVATLEERLYRGFTERRRKEIERAARRKRYKRFGPEDPR
                     LPKNW"
     gene            complement(13295..13735)
                     /locus_tag="PLS34_03385"
     CDS             complement(13295..13735)
                     /locus_tag="PLS34_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRP86547.1"
                     /translation="MTTEILYRSKVDGWFRTTLLLSALCCTIAVLAPGLAGAPLLSLA
                     VSPMLLVGVGLPLWLLSSTCYLLRERELEARCGPFRWHIPLAEIHGLARTRSWLASPA
                     LSLDRIRIDYGQDRWIMVSPDDPESLLAELQRRISIQARPTAAG"
     gene            complement(13830..>15002)
                     /locus_tag="PLS34_03390"
     CDS             complement(13830..>15002)
                     /locus_tag="PLS34_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRP86548.1"
                     /translation="AEVARTLGCNRRQVFFRVGLPLARPAIAAGTGLALMEALADFGT
                     VQHFAVRTFTTGIYRVWLTMGDRVAALQLAACLMGVVALLLALERAARGPQRFHNTSS
                     KHQALTRSPLGGATGTAAMLACALPVMLGFLLPAYVLLRYALTEGDPLFGPTILPFAR
                     NSLMLSGVAALIATAIAVVVAYGVRLRGSFTMRAAARTAAMGYAIPGSVIAVGVLIPL
                     TLADHGLRGWLTSWFGIDSGLMLTGGILGLLFAYVARFLAVSLQSVDAGLARITPAMD
                     DAARALGEGPGRTLRRVHLPILRANLLTAGLIVFVDVMKELPATLILRPFDFDTLAVQ
                     AYKLAADERLAEASTASLAIVVAGLIPVILLARQVSRPAQRRKRSEVAPLMAPATA"
BASE COUNT         2172 a         5140 c         5242 g         2448 t
ORIGIN      
        1 gaactcgtgg aacatggtgg tgacctcggt gaaggtcatc agcgtcggct cgccctcggg
       61 cggcttggga atgttctggt ggttggccac cacgggcttg gtgccctcca ggccgttctg
      121 cggcacgtag gcgttcatcc aggcgccgcc gcgcttggag tcgcgggcgt agaagtcgcc
      181 gatgaagatg cccagcggcg taccgtccgc ctcgaagacc tcccaggcgc gcgcgtccgg
      241 gtggtacagc ggcaggtcgg gccgcggctt gaaggtcagg ccgaacacct ggttggcggc
      301 atagaacacg ccgttgttga gcacgctctc cagctcgaag tagggcttga gctgtgccgg
      361 gtcgaggtcg tagcgggcct gcttcacctg ctcggcgtag aagtcccagt cccaggcctg
      421 cagctggaag tcgccgccct gcgcgtcgat cagcgcctgg atgtcggcgg cttcgcggcg
      481 cgcgttggcg accgccgccg gcgccagccc ggtgagcatg ctgttgaccg cgtcggtggt
      541 gccggcggtg gcgttctcga gcacgaaggc ggcgtgattc gggtaaccca gcagcttggc
      601 ccgctcggcg cgcagcgcgg ccgtgcgcgc gatcaccggg cggttgtcgt tctcgttgtc
      661 acgggcgccg cggttcaccg aggcctcgtg gatgcgctgg cgcagcgccc ggttggtgag
      721 cgagctgagc ggcggctggc cggaggtgtt ctgcagcgtg acgagatact tgccctcgag
      781 gccgcgctcc ctggcggcat tggccatgcc gcggatctgg ctctccgtga gaccgtccag
      841 ctcttcggcg gtgtccacga cgacggccga gttgatggtc tcctgcagca cgttctgcgc
      901 gaactgcgtg gccagggagg ccagctcgcg gttcagctcg cgcagcttct ccttgtcggc
      961 ctcggagagc cgcgcgccgg cgcgcacgaa atccgtgtgg tagcgctcca gcaggaactt
     1021 cgactccggg tcgaggccga gctcgtcgcg cctggcatgc agcgcctcga cgcgtgcgaa
     1081 cagtgcgccg ttcaggtaga tggcgtcctg gtgggccgcc agcagcggcg agacggtctt
     1141 ctgcacttcc tgcatctgcg cgttggtgtg cgccgagttg aggttgaaga acacgcgcga
     1201 aacccgctcg aggatcgggc cggtgcgctc catcgcgacg atggtgttct cgaaggtcgg
     1261 cggttccggg ttgttggcga tcagctcgat ctcggcgagt tcctcttcca tgccgcgctc
     1321 gaaggccggc aggtagtggt cgtcggtgat ctggtcaaaa ggcggcacgc tgtagggcag
     1381 ggtgctggcc gtgaagaagg ggttgggagc gacgtccttg ccggccatgg cggcctcggt
     1441 ggtctctgcg atctggtcgg cggccttgtc ggcggcggcg gcaggcgcct gaggctcggg
     1501 ctgcttgccg caggcgacca gcagggcggc ggtcgcaagg gccaggggaa gggcgaagcg
     1561 tcgtgtcata tggtctctcc ggtgtggctc aggcgccggg cgcccggtcg aaaagcggcc
     1621 attctacccg ctcggggggg atttccaggc attcggcgcc aaaatggcct ctgttatagt
     1681 ccccttcatg ctccctgccg ccaccctcga gggcctcaac gaggcccagc gccgcgccgt
     1741 gaccctcggc acgcggcggc cggacggccg cgggctggcg gcgccgcccc tgctggtggt
     1801 ggcgggcgcc gggactggca agaccatgac cctggcccac cgggtgggac acctggtgct
     1861 cgccggggtt ccgccggagc gcatcctgct gttgaccttc agccggcgag cggcccgcga
     1921 aatgctgcac cgtgcccggc gcatcgtcgg ccaggcgctg gccgaccagg gccgcggcga
     1981 ggcagtccag cttccctggg cggggacctt ccactccgtc gccaaccgcc tgctgcgcga
     2041 gtacgcccgc aaccttcggc tggaccccgg cttctcggtg ctggatcgcg gcgacgccgc
     2101 cgacctcatg gacgtggcgc gccaggagct cgagctcggc gaggccgcgc gccgcttccc
     2161 gcgcaaggac acctgcctcg tgatctattc ccgcacggtg aacgcgcgcg ccgcgctcgc
     2221 cgactgcctg gccgagggct ggccctggtg cgaggactgg cgggacgagc tgcgccggct
     2281 gttccgccgc tacgtggagc tcaagcaggc cgacagcctg ctggactacg acgacctgct
     2341 gctgtggtgg cacggcctga tggaatcgcc gccgctggcg gcggcggtcg gcgagcgctt
     2401 cgaccacgtg ctggtggacg agtaccagga caccaacgtg ctgcaggccg agatcctgct
     2461 gcggctgaag ccagacggtc gcgggctcac ggtggtcggt gacgacgcgc agagcattta
     2521 ttccttccgc gccgcggagg tggagaacat cctgcggttc cccggccagt tcacgccgcc
     2581 ggccgagatc gtcacgctgg aggagaacta tcgctccgtg cagcccgtgc tggatgcagc
     2641 caatgcgctg atgcgcccgg ccgagcgcca gttccggaag gacctgcgct cggcgaagcc
     2701 ctccggacag aagccgtatt acgtcaccgt gaccgacgac gatgcacagg ccgactacgt
     2761 ggtgaatgcc atcctcgctg cgcgcgagac cgggcagcag ctgcgccacc aggcggtgct
     2821 gttccgcgcc agtcatcaca gcgatcggct ggagctggag ctcgtgcggc gcaacatccc
     2881 cttcgtgaag tacggcgggc tgaagttcct cgaggcctcg cacgtcaagg acctgctggc
     2941 ggtgctgcgc tgggcggaca atccgcgcaa ccgcatcgcc gccttccgca gcctgcagct
     3001 gctgccgggc atcgggccgg cgctggctgc cagggccttc ggctggctgg agtcccacgg
     3061 cttctcgccg gcggcgctgg ccgactggcg cccgccggcc gcggcggcgc cctactggcc
     3121 gggactggtc gagctgctcg ccgggatcgg cgccgacgag ggcgactggc ggccgcgccc
     3181 gcgactggta ccggccgcag ctggagcgcc tttacgagga atggccggtg cgcctcggcg
     3241 acctcgatgc gctggagcag atcgccgggc gctacgcgac ccgcgagcag ttcctcaccg
     3301 agctgtcgct cgacccgcca caggccagcg gcgatctcgc cggcccgccg ctgctggacg
     3361 aggactacct ggtgctgtcc accatccact ccgccaaggg gcaggagtgg gactcggtgt
     3421 acgtgctgaa cgtggccgac ggcaatttcc cctccgagtt cgccaccggc aagccggcct
     3481 tgatcgagga ggaacgacgg ctgctgtacg tcgccatgac gcgcgcgaaa tcgtcgttgc
     3541 acctggtcgc gcccttgaag tactgggtgc cgcagcagcc gcgccacggc gaccggcacg
     3601 tctacggtgc gcgcagccgc ttcatcgacg acgccatgat ggcctgcctg gaaaagcgct
     3661 ttcacggcgc cgctgaggcg ggcgtgagcg ggtcgccgcg gcccgccgaa gtcgtcgacc
     3721 tcggcgcgcg catgcggacc atgtggtgag cggcggatca tgagcgtgct gggcatcgac
     3781 atcggcggct cgggcatcaa gggcgcaccg gtgaacctcg tcgagggccg gctggccacc
     3841 gcccgccaca agctgccgac gccgcagccc gccacgccgg aggcggtgac ggcgtccgtg
     3901 gtcgagttgg ccgggcagtt cgagctgcgc ggcagtgtcg gcgtggccgt accggcggtc
     3961 gtcaagcacg gcgtcaccca gaccgccgcc aacattgacc cggcctgggt cggttgcgac
     4021 atcggccgcc tgctgcacga tgcgctcggc cggccggtcg tggtgctcaa cgacgccgat
     4081 gcggccggcc tcgccgagat gcgctacggc gccggccgcg gccggcgcgg cacggtggtg
     4141 atgctgacgc tcggcacggg catcggcagc gccctgttcg tggacggcgt gctatggccc
     4201 gacaccgagt tcggccacct ggacgtccgc ggcaaggcgg ccgagcatcg cgcagccgcg
     4261 cagctgcgca aggccgaagg gctgagctgg gagaaatggg cggcgcgcgt ctcggaagtg
     4321 ctggttgcca tcgagcgact gctgtggccg gacctctaca tcatcggcgg cggcgtgagc
     4381 cgcaagcagg agaagttcct gccgctgctc gagtgccgca caccggtcgt gcccgcggcc
     4441 ctcggcaacg acgccggcat catcggcgcc gcgctcgccg cgatcgggga cactccccac
     4501 caatcccatt gaagagcctc ggcacgatat tgctgttcgt ggcgctgccg tacgcggcga
     4561 tggtggcgtt tctgtggctg cggcaggaaa gcatgctgtt cctgccgcac atgccttcac
     4621 gcgacatcgt cgccacgccg gccgatatcg ggctgcgcta cgagccgctg cagctgccga
     4681 ccgccgacgg cgagacgctg gctgcctggt tcgtgccggc gcccgaggca cgcgccgtgc
     4741 tgctgttttt ccacggcaac gccggcaaca tttcccaccg cctggattcg ctgcggctct
     4801 ttcacgacct cgggttgtcg gtgctgatca tcgattaccg cggctatggc cagagcaccg
     4861 gccggccaac ggagcccggc acttacgagg acgcgcgtgc ggcctggcgc cacctggtgg
     4921 aggagcgcgg tattccggag cagcgcatcg tgctgttcgg ccgctcgctc ggcggcgcga
     4981 tcgcgacctg gctggccgcc cataccgcgc cgcgcgcgct gatcgtggaa tcggccttcc
     5041 gctcggtgcc cgacatggcg gcggagatct actggttcct gccggtccgg ctgctggcgc
     5101 gcctgcacta cccggtggag tcgctattgc cgcaggtcac ggcaccggtg ctgatcattc
     5161 acagtcgcga cgacgagatc attccctttg ctcatggcga ggtactgcac gccgctgcgc
     5221 ccgaaccgaa gaagctcctc gagctgcaag gcgatcacaa caccggcttc ctgcagagcc
     5281 gttcgcatta ctccgccggc atcgacgcct ttctcagcag tcacgggctg taaggctgga
     5341 attggggaat gttcccaatt ccgggtcaca tcccaagcgc ccaggcgaag aaggcgtaca
     5401 gctcggcgaa ctcgtcgatc agcaggtagc gcggcttggc gccgcccgag gcgtgcccgg
     5461 cgcgggtctc ggtgcgcagc agcaccggcc ggtcgcagcc ctgggcatgc tgcagggcgg
     5521 cggcgaactt gtagctgtgc cagggcacca cgcggtcgtc gaacttgccg gtgtggatga
     5581 tggtcgccgg gtaacaggtc ccgggacgca cgttgtgcac cggcgagtag gccagctgcg
     5641 cgcggaagtc cgtttcgttc tccgacaggc cgaagtcgcc agcccaggcg cgcgcattgg
     5701 cgctcggcgt gtggtaacgc agcatgtcga gcacgccgac tgccggcccg gcggcggcga
     5761 acagctccgg ccgctgcagc tgcaccgcgc cgatcagcag gccgccgttg ctgcgcccgt
     5821 agatggccag cttgtcgggc gaagtccacc gctgcgcgat cagccattcc gccgccgcga
     5881 tgaagtcgtc gaaagtggtc tgcttgcgct cgcgggtgcc cgccaggtgc cagtccgcgc
     5941 cgtactcgcc gccaccgcgc aggttggcga tcgccagcac gccgccgagg tccagccagc
     6001 cgagcagccg caggtcccag tccggctgca tggacacccg gaatccgccg tagccgtaca
     6061 gcatggtcgg attgcggccg tccagttcga tgcccttgcg gtgcacgatg aacatcggca
     6121 cccgcgtgcc gtccttgctc tcgtaaaaca cctggcgcgt ctccaggacg ctggtgtcga
     6181 gccccgtggc cttctggtgg aagcgcccca cctcgccgtc cgccgagcgg tagcgataga
     6241 tggtgcccgg gttgtcgaag ctctcgaact ggtagaaggt ctcggcggat tccggcgagc
     6301 ccgtgaaacc cgacaccttg ccgatgcccg gcagggccag cgcgccctgc ggcgtgccat
     6361 ccagcgcgaa cagttcgaca cggctcttcg cgtcctccat gtagcgcacg atgagctgcg
     6421 cgccgatcag cgacacgccc tcgataggca gtgcgctttc cggcacgact tcccgccagt
     6481 cggcctgcgc cggccgcgcc aggtcgatgg ccaccacgcg gccgcgcggc gcgccgatgc
     6541 tggtgctgat gtagaccgtg tcgccgacgg tgccgagata gcccgcgcgc ccgtcccagc
     6601 tgtccaccag gcggatcacc tcggcgccgg gctcgtccag cggcagcagg tagatgccgt
     6661 tggcgcggct gtcctggccg atgccgagca ccaggtgacg gccgtccgag gacgcctgcg
     6721 ggaacggcgt gcgggtcggg tgatccgtga tagagaacac gtgggtgtcc tcggcctggg
     6781 gcgtgccgat gcggtgcaac cagacctcgg cctgctggag gtcgtcccac tcgcccgcgg
     6841 cgttcttcgg atagcgggcg tagaaaaagc ccgatgcgtc cttgagccag gccgcgctgg
     6901 tgaacttggt gccctccagc ccgtgcggca ggtcctcgcc ggtatcggtg cgacgcacct
     6961 tccagcgggt ccagtcggag ccgccgaccg tggcggccca cgcgacgtat tgcccgtccg
     7021 ggctgacgtt ccagcgcgcc agcagcgtgg tgccgtccgc ggacagcgcc tccgggtcga
     7081 gcagcatgcg ccactcgccg tccagcgagt cggcgaccat cagcttggac agcgtcgccc
     7141 ggccgtcgcc gtactcgaag aagtagcggc cgtcgtggac gcgcggcacg ctcatgcgct
     7201 cggtggtcca gagcacttcg aggcgtgcac gaatccgctc gtagtcgggc agcgcttcaa
     7261 ccaccttcgc cgagtactcg ttctgcgccg cgacccaggc gtccacttcg gccgagtcga
     7321 gctgctccag ccagcggtaa gggtcggcca cccgggtgcc gaagtactcg tcgacatggt
     7381 tgacgcgacg ggtctcggga tagcccggcg ccgccgggcg gggggacgtc gcggcgcaag
     7441 ccgccagggt cgcgcacagg agcgcgacgg tgattttctg ctgcatcggt ttacctcagg
     7501 ccggtttcct ggcgggcgat gaccatgcgc tggatctcgc tggtgccctc gtagatctcg
     7561 gtgatcttgg catcgcggaa atagcgctcg atgggcattt ccttactgta gcccatgccg
     7621 ccgtgaatct gcaccgccgc atgcgtgatg aacatggcgg cctcggaagc gaacagcttc
     7681 gcgcaggagg cctcggtggt attgcgcagc ccggccgccg cggcgacctt cttgttccag
     7741 gccgccttga gcgtcagcag gcgcgcagcc tcgaggcgca tcttcatgtc ggcgatcctg
     7801 gcctggatca tctggaagct gccgatcggc gcgccgaagg cgtggcgctc gcgcacgtac
     7861 tcgaggctcg cctcgtaggc ggcgcgcgcg atgccgacgg cctgcgcgcc gatgccgatg
     7921 cggccggcgt cgagaatgct catggcgatc ttgaagccgt cgccctcgtt ggcgaggcgg
     7981 tcctcgaccg ggcagacgta gtcggcaagt tcgatctcgc aggtggccga ggcgcggatg
     8041 ccgagcttcg gctcggtctt gccgcaggcg aagcccttct tttcagtatc gatcatgaag
     8101 gccgaaacgc ccttcgggcc cgccgacggg tcggtcatgg cgaacagcat cacgtacttc
     8161 gccaccgggc cggaggttat ccaggacttc ttgccgttga tcacgtagtg gtcgcccttg
     8221 cgcaccgcgc gggtgcgcat gttggcggcg tcggagccgg actgcggctc ggtgaggccg
     8281 taggcggcga acacttcgcc cgaggccacg ggcgtgacgt atttttgttt ctgttcctcg
     8341 gtgccgtact gcaggatgcc gttgcaatac agcgagttgt gcacggagat gacggtggag
     8401 tgggcggcgt ccgccgccgc gatctcctcc atcatgagcg caaagccgat cgcatccagg
     8461 ccggcgccgc catactcctc cggcacttcg acgcccatga agccgagctc gccggccttg
     8521 cgcagcgtct gttccggaaa ctggccgctg gcgtcgaact cggctgcgat cggcgcgatc
     8581 tcgcgctgcg cgaagtcgcg cgcggcctcc tggatcatct gctgctcttc ggtgaacttg
     8641 aagtccatgg cgggtctccg gacaaggcgg taaacaatcg ttcgattcta acccgcacgg
     8701 cggtttcggg ctaagccggc tcgtagcgga cctcgagcag cagccaggtc ttgcgcccgg
     8761 cggccagctc cagcgtgacc tcgtcccccg ggctgcgctt catgagtgcc cgcgccagcg
     8821 gcgcgtccat gctgatgtac ccgggcgcat ggtcgaactc gtcgggcccg acgatgcgga
     8881 agcgtgattc ggcgccggtc tcgtcttcga ccgtcaccca ggcgccgaag aacacctgcg
     8941 tggtgtccgc cgggctgcgg tccaccacct tcagcgcgcc gagtcgccgg cgcaggtggc
     9001 ggatgcgccg gtcgaggccg ccgagctgct tcttgcggta gatgtactcg gcgttttccg
     9061 agcggtcgcc ctcggccgcc gcgtccgcga gggcgcgcac cacctcgggc cggtccacgc
     9121 gccacaaccg gtcgagttcc tcgcgcaggc gggcggcgcc ctccggcgtg atgtaaggcg
     9181 aggacttcgg ctgcggcggg cggtaacggc tcatcgggca ctatactcgc acgccagggt
     9241 ccaatgccga cgaagcgtgc agcgtggcgc agcccggcag cgctgttacc gacaggagag
     9301 ccatcacgtg aagaacttac tgattgtgcc gctgctgctg gcctggtgcg gcgtggcgac
     9361 cgccgccgac gaggtggaat ggttcagcaa gggcccgggc ggcgaggacc ggatcgtgat
     9421 gcactatttc tattcggaca cctgcccggt gtgcaccgag gccaagccct tcatcgaggc
     9481 cctgcgcgag gtcgcctggc tcgaggtacg ggccttcgag gtttccgcca atcgcgacaa
     9541 cgccatccgc taccgcgaca cggccgccgc catcggcggc caggcgcgca gcgtcccggc
     9601 ctacatcgcc tgcggcgcca tgctcaccgg ctacaacggg ccggagccga cctgggagcg
     9661 gctgtcggtg atcttcaagc gctgcatgcc ggcaggcggc gagccgtccg ccgccgagac
     9721 cccgcccgcc gccggggact agatgcattt cctgctgctg acgatcctgc tgctgggcct
     9781 ggtgttcggg ccgggcctgt gggtgaaaca cgtgctggcg aagtacgcga cgccggccga
     9841 tcgctatcgc ggcagcggcg cgcagctggc gcgcttcctg ctggatcgcc gcggcctgga
     9901 atcggtcgcc gtcgagcaga ccgagcaggg cgatcactac gaccccctcg ccaaggccgt
     9961 gcgtctgtcg cccgacaact ttcgcggccg ctcgctgacc gccatcacgg tggccgcgca
    10021 cgaggtcggc catgccgtcc aggaccacac cggctacccc ccgctgaagc tccgccaccg
    10081 gctggtcacg tccatgcggc acgtcgagcg gctcggcgcc ggcctgctga tggtctcgcc
    10141 cttcatcggc atgctgacgc gcgcgctgat cgtcggcatc gtgatgttcg tcggcggcct
    10201 gctgacgctg gggacggcga cgctggtgca cctggtgacc ctgccgacgg aaatcgacgc
    10261 cagtttcggg cgcgccctgc ccatgctgga agaagaggag atcctcatcg aggacgacct
    10321 gccgcacgcc gagcggctgc tgcgcgccgc agcattcacc tatgtggcgg cctcgttgat
    10381 gagcctgctc aacgtggctc gctggtgggc tatcatccgt cgctgagccc ccgcccgatc
    10441 ccctgccgcg aaaggaatcc cgacatgtcc accgtcaagg cgtacgccgc ccagtcaccg
    10501 acctcggccg tcgagccctt cgccatcaag cgccgcccgc cgcgtccgga cgacgtggtg
    10561 atcgagatcg actactgcgg catctgccac tcggacatcc acaccgcgca caacgactgg
    10621 ggcttcacga gttacccggt ggtgcccggc cacgagatcg tcggccacgt cacggcggtc
    10681 ggcaacgaag taaagaactt caagcccggc gatcgagttg gggtcggctg cctggtggat
    10741 tcatgccgca ggtgcggctc ctgcgagagc gggctggagc aatattgcga gcgcggctgg
    10801 gtgggtacct acaacggcaa ggacctgcac gacggctcgc cgacctacgg cggctactcc
    10861 gagcggatcg tggtcagcga gcgcttcgtg ctgcgcatcc cggacacgct cgacgcggcc
    10921 gccgccgcgc cgctgctgtg cgccggcatc accacctggt ccccactgaa gcatttcggc
    10981 gtcggcaagg ggcatcgcat cggcgtggtc ggcatgggcg ggctcggcca catgggtgtg
    11041 aagttcgcgc gcgcgctcgg tgccgaggtg acggtcttca cccgcaccgt cgccaaggtc
    11101 gacgaggcga agaaacaggg cgcgcaccac gtgatcgtct ccaccaacga caagcagatg
    11161 gccgcggccg ccgcgagctt cgacttcatt ctcgacacgg tgcccgtgcc gcacgacctc
    11221 aatccgtatg tgaacgcact caagcgcgac ggcacgctga tcctggtcgg cctgctggag
    11281 ccgatcgaac cggcgctgca ggccttcccg atcgtgctcg gccggcgccg catcgccggc
    11341 tcgctgatcg gcgggctgcc cgagacccag gaaatgctgg atttctgcgc cgagcacggc
    11401 atcgtctgcg acatcgagat gctagacatc cgcaacatca acgaggcctg ggagcgcacc
    11461 cagcgcagcg acgttcgcta tcgcttcgta atcgacatgg ccacgctgag ggattgatgc
    11521 gtcgaatggc gctgcgcctg gccgttgccg ccgtggtgct gctggcgctc tcggtgccgg
    11581 cgttctggcc gcagtacctg tcgaggccgg gagaatcgga cgcctacacg cattttcacg
    11641 ccggcctcgg gctggcctgg ctgctgctgc tcatcggaca gccgctgctg gtgcgtgccc
    11701 gccggctgga cctgcaccgc cgcatcggcc gcgtcggatg gctgctcggc gcagcattcg
    11761 cggtatccgg cgtgctggtt gcgcaccgtg gcgtggcccg catggacgag gccgaactgg
    11821 cgcgcgacgg ctttttcctc tatatgccgc tcgcgcagtc ggcattgttc gcagtcgccc
    11881 tggcgctcgg catcgcgtgg cgcagtgaac ccgcgactca cgcccgctac atggcctgca
    11941 ccgcgctgcc gctgctcgat ccgctgctgg cccgcctcct gcatttctat tttccgccct
    12001 tgccggccgg tttcctttac cagttgccgg cctttttcct caccgtcggc gtgctcgccg
    12061 tattgtccgg gtcgatgccc cgatcgtcgc ggggccgtgg cggattcagg gcattcgccg
    12121 cgggcaccat cgtcacgttg ttgctattct tcgtcacgcc ctacagtgcc gcatgggcga
    12181 cattcgtggc atggttccgg gcgctgcccg tgacttgagc atgtgactgg aggagaacgc
    12241 gtgatcgagc ctgacgacca ggaggcttcg accacgtcga gccggctgag gcacggaagc
    12301 ctgctcctgc tcggactcgc cgtcgcctac agcccgagtt acatcaacgt cgcgctgggc
    12361 ttggcgggcg tggagtggga gttcaccaag ggccccccgt cggtgctgct gtggaactgg
    12421 ctggcggtcg ccgtactgct ggtcttcatc ctgaaggtgg aaggccgtgg gctggggtcg
    12481 atcgcgctgc ggcggccgag cggccgggac atccagtggg cggtcatgtt ctgggcggtc
    12541 tcgacggtcg cgtcgggaat gctgcaccag ttcctgccgc cgccgccgtc caccggcatg
    12601 gcgaccgtgc tgcagctgtc tctgctgacc atcctcgcgc tcgtattcac cacggcggtc
    12661 accgaggagg tcctgttccg gggctattcg atcgagcgtc tgagtgaatt gacgggctac
    12721 cggccgctcg ccgtggcggt gagcttcggc ctgttcgtgc tgccgcacgt ggcgtttttc
    12781 ggggtccaat ggctcctcta tcacggcctg ggcgtcgtgc tgctgtacgt gctgtacgtc
    12841 tggcgccgca acctttgggc gtgcatgatc atgcacttgc tgggaaacgc gctgctgctg
    12901 atcccggccc tcggcctggc ggactgatcc gccgggcccg ggcgaggagc accggaatga
    12961 tcggaaaccg attcgagtac ggaccgctgg cgcgcggcat ctacatcgcc ctcgcctgga
    13021 tgaccccgct cgtggcgatc gtctggtggg agctgctgct ctggcacttg ctgctgctcg
    13081 cctttctcgg cctcggcctc cggccgctgc tcgagaagac cggcatccac gcgctctacc
    13141 agcacgtcgt ggcgaccctc gaggagcgcc tgtaccgagg tttcaccgag cgacgccgca
    13201 aggagatcga gcgcgccgcc aggcgcaagc gctacaagcg cttcggaccg gaagacccgc
    13261 gcctgccgaa gaattggtaa tgcgtgtccg ccgctcagcc ggcggcggtc ggccgggcct
    13321 ggatgctgat gcgtcgctgc agttcggcga gaaggctctc cggatcatcg ggcgacacca
    13381 tgatccaacg gtcctggccg tagtcgatgc gaattcggtc gagcgacagc gcgggactgg
    13441 ccagccagct gcgcgtcctg gccaggccgt ggatctccgc cagcggtatg tgccatctga
    13501 acgggccgca acgggcctcc agctcacgct cgcgcagcaa gtagcaggtc gagctcagca
    13561 gccacagcgg caagccgacc cccaccagca gcatgggcga aacggccagt gacagcagcg
    13621 gtgctcccgc caggcccggc gcgagcactg cgattgtgca gcacagggcc gagagcagca
    13681 gcgtggtgcg gaaccagccg tccactttcg agcgatacag gatctcggta gtcaaggcgt
    13741 cgtctctggc gcggtcagct gggatgagtc tcggcgccca cagtgtagag cagcggcggc
    13801 caatttgaag cgggagcgtc cggcctcgct caggccgttg ccggcgccat cagcggcgca
    13861 acttcgctgc gcttgcgtcg ttgcgccgga cgcgagacct ggcgcgccag caggatgacc
    13921 gggatcaggc ccgccacgac gatggccagc gaggccgtcg aagcctcggc gaggcgctcg
    13981 tccgcagcca gcttgtaggc ctgcaccgcg agcgtgtcga aatcgaaggg cctgaggatc
    14041 agcgtcgccg gcagttcctt catgacgtcc acgaacacga tcaggccggc cgtcagcagg
    14101 ttggcccgca ggatcggcag gtgcacacgg cgcagcgtgc ggcccggacc ctcgccgagg
    14161 gcgcgggcgg cgtcgtccat cgccggcgtg atgcgcgcca ggccggcgtc cacgctctgc
    14221 agcgagaccg cgaggaaacg cgcaacgtaa gcgaacagca gcccgaggat gccgccggta
    14281 agcatcagcc cgctgtcgat gccgaaccac gaggtcagcc agccgcgcag cccgtggtcc
    14341 gcaagcgtca gcgggatgag cacgccgacc gcgatcaccg agcccgggat cgcgtagccc
    14401 attgccgcgg tgcgcgcggc cgcccgcatc gtgaagctgc cgcgcagccg cacgccgtag
    14461 gcgaccacga ccgcgatcgc cgtggcgatc agcgcggcca cgcccgagag catcaggctg
    14521 ttgcgcgcga agggcaggat ggtcgggccg aacagcgggt cgccctcggt cagcgcgtag
    14581 cgcagcagca cataggccgg cagcaggaag ccgagcatga ccggcagcgc gcaggcgagc
    14641 atggcggccg tacccgtcgc gccgccgagc ggcgagcgcg tcagcgcctg gtgcttcgag
    14701 gaggtgttgt ggaagcgctg cggcccgcgc gcggcgcgct cgagcgcgag cagcagcgcg
    14761 accacgccca tcaggcaggc ggcgagctgc agcgccgcca cccggtcgcc catggtcagc
    14821 cagacccggt agatgccggt ggtgaaggtg cgcacggcga agtgctgcac cgtgccgaaa
    14881 tccgccagcg cctccatcag cgcgagcccc gtgccggcgg cgatcgccgg ccgcgccagc
    14941 ggcaggccga cgcggaagaa cacctggcgg cggttgcagc cgagcgtgcg cgcgacctcg
    15001 gc
//
LOCUS       DAOMPG010000038        14967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1727_length_14967_cov_4.710502, whole genome shotgun sequence.
ACCESSION   DAOMPG010000038 DAOMPG010000000
VERSION     DAOMPG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_14967_cov_4.710502"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLS34_03395"
     CDS             <1..966
                     /locus_tag="PLS34_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRP86549.1"
                     /translation="GQVRAEAERSLKLPAVQVLGEHAARLPESGVDAFVPAAGGGFRE
                     IRYEERDAVGWLHFDFHNGAMNTAQCRRLLAAWRHAAARPTRVIVLAGGRDFWSNGLN
                     LNVIEAADSPADASWDNINAIDDLAEAIVRETGKLTLSMVGGNAGAGGVFLALAADHV
                     LLRDGVVLNPHYRNMGNLYGSELWTYLLPRRVGESGVRAVMDHRLPMLARTAVASGLA
                     DELGPGEVEAFAAHAKARALTLAALPDWERRLADKQRRRAADEAERPLSDYRAAELER
                     MKRNFYGFDPSYHVARFRFVHRNPHAWTPLYLAAHRRLRGPSPRH"
     gene            1065..3080
                     /locus_tag="PLS34_03400"
     CDS             1065..3080
                     /locus_tag="PLS34_03400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRP86550.1"
                     /translation="MRKIVAVAFVLALSLAAPAGAKPLDAGTLWQLQRLASPAISPDG
                     KLAVVAVTRYEVPEDKGITNLWLVPTDGGAARPLTTHPGSDSSPAWSPDGRHIAFVSR
                     RGDDEQSQLYVIPLGGGEARRVTKVPTGASAPKWFPDSTELAFISRVYEDADGWDDMV
                     ARLKAEKDSKVSARTWDTAPLSFWDRWHDARRPHVYRVSVDGGEPRPVTLGSGVHLLI
                     SSADAASYDVSPDGAELAVVADSDASGVESNPDIYLVPAAGGAARNVTPDSPAPDFAP
                     VYSPDGRWLAFSQQRIPGFYGDRARLVLHDRRDGAHRVVTEDFDRSVSGVLWAPDSRN
                     VYAAIDDAAHERIYAIDTRNGATRAITREHSFGSPAMSQDGRVLVALRQSFIEPPTLV
                     RVEPRRGDVTKLSSFNDELLAGVDFGRYESVTYEGANGAPIQMWVVYPPGFDPEKKYP
                     LYLLLHGGPHNGIPDGFHFRWNAQVFSGWGYVTAWHNFHGSSGFGQDFTDAINPNQSE
                     LPYVDTIKAAEWFARKPWIDAERMAAGGGSYGGYLASILLGRDHPFRTLIAHAAVFNW
                     KTQYAADYGASRRRHGEYWVNPEHYELSSPHMGAANFDTPTLVIHGQLDYRVPLNHGI
                     ELFNILQNRGVRSRFVYYPDENHWILKPNNSLHWYREKQDWLAEFLR"
     gene            3272..3517
                     /locus_tag="PLS34_03405"
     CDS             3272..3517
                     /locus_tag="PLS34_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP86551.1"
                     /translation="MLTAAQLRAARALLDLSQRQLAEAAGVSVPTIRRMEASAGTVRA
                     NVDTLVKIIDALDVLGVELLGEGTPSLGGGRGIRLKQ"
     gene            3514..5562
                     /gene="hyfB"
                     /locus_tag="PLS34_03410"
     CDS             3514..5562
                     /gene="hyfB"
                     /locus_tag="PLS34_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HRP86552.1"
                     /translation="MTATDMLPMFVGLALGGSLLAAVLGLFTGRKRPAMQVLFCASLG
                     AGGLAGTAAGALMLSGAGGFGVELPVGLPWLRLHLQLDALGGTFLGLIGMVTIAVSMF
                     APRYTREYQRRRHSPGALGLATGLFVAGMQFVVLAGDAFGFMIAWELMSVASYLLVAY
                     EHEESAHRRAAFIYLLMAQIGALLILLAFGVLSGFEGDFTFDALREKALEPPWPGIAF
                     ALALLGFGMKAGLVPLHVWLPEAHPAAPAHISALMSGVMLKVAIYGFIRFSFDLLGAP
                     HWSWGVAVLLAGSITALYGVLYAIMQQELKRVLAYSSIENVGVIFIGLGLAITFIGTG
                     HPALAALGLVAALYHCVSHALFKGLLFLGAGAVVQASHTGTLDQMGGLIRVMPWTALF
                     FLVGCLGSAALPPFNGFVSEWLIFQTALQATVLDDGVLRAVIPVTAAMLALTGALAAA
                     CFVRAFGIAFLGQGRTRRVRHAREVSRGMIAGQGLLALLCLLFGILPTFAVAGLGRVA
                     EGLTGYALSSATDRGWLWLTPVAPEVASYSAPLVFLGVGGALAVWAIAYLAVRRQRHG
                     APMPRSDAWDCGFGGTSARAQYTATAFSMPIRQVFEPVFELQEQTVREMQPELVTRPA
                     HIRYLLHVEDRAWKIFYRPVERAVQRAARLIGRIQTGQLRHYLIYSFVTLLLLLWLIT
                     "
     gene            5547..6512
                     /locus_tag="PLS34_03415"
     CDS             5547..6512
                     /locus_tag="PLS34_03415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRP86553.1"
                     /translation="MADHLNPVAWLLALLQTLMFVLAAPVFAAWVKRVKCRLQNRRAP
                     SLLQPWRDIRRLFAKRLVLAHTASPVFRVAPYIVCGTTIMAASVVPLVALTLPLAAMA
                     DAIVLVALIALGRFFLALAGLDVGTAFGGMGSSREMTVSALAEPAMLMVVFTLAMIAS
                     STNLTTVVTHMLVEAQALRPSLLFALIALILVGIAETGRIPVDNPATHLELTMIHEAM
                     VLEYSGRHLAMMDLAAQLKLMIYAVLVANLFFPWGIAIENTPAAWLIATGLLLCKLTL
                     LGIGLAVAETAVAKMRLFKVPTFLAAAFIIALVGMLSALILEGGA"
     gene            6509..7189
                     /locus_tag="PLS34_03420"
     CDS             6509..7189
                     /locus_tag="PLS34_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HRP86554.1"
                     /translation="MTPYLELPLVEQAILTVAALVLVSSFALLAQSRVVATIHIFAWQ
                     GGLLATGCALVAWSTGETHLYLSAGLTLLLKSAFIPWLLVRQARRLGILKEFDAVIRP
                     GVTLLAGGAMVIFCYGVAQPLGAYAEGTTRDAIALSLAVVMLSMLMLVTRRKALTQVV
                     AFMALENGMFFAAITATRGMPMIVELGIAFDVLVAAIIFGIFFFHIRDSIESLDIEQL
                     NRLSEKEP"
     gene            7186..8661
                     /locus_tag="PLS34_03425"
     CDS             7186..8661
                     /locus_tag="PLS34_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HRP86555.1"
                     /translation="MSPLLATLLAPALAALLLAFLSSPRTGALLNIAASSCGLAAALW
                     LAVEVLRLGAIGGEQFYVDTFNVYLIVLTAFIGLTTTFFSRPYMRHELEIGRVTGRRL
                     RLYHASFQGFLFSMLLALSTNNLGVLWVALEAATLATVLLVSLYRTPESVEAAWKYFI
                     LCGVGIAQALFGTVLLYLAAARVIEDPDQALLWSVLHAHADQLEPVVMSIAFIFLLVG
                     YGTKVGLVPLHNWLPDAHSEGPTPMSAVLSGLLLNVALYAVVRVKMLTDAALGADAGE
                     PGLAGFLMMGFGLASFIVAGLFLHRQRDIKRLYSYSSIEHMGLMTFAFGLGGALATFG
                     ALLHMTVHSLAKSAIFVTVGHASQIAGTQSIDAIRGLIRTQPAVGWGLLVGSLAIAGF
                     PPFGVFVSEFLVLTATMQAYPWLTVPLLVGLAVAFAGLFRHLQPMVYGPCPPGQEPIR
                     VSMAPVILHLAIVLWLGLAIPAFLSRWFDEATRLISGSGLL"
     gene            8658..10235
                     /locus_tag="PLS34_03430"
     CDS             8658..10235
                     /locus_tag="PLS34_03430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRP86556.1"
                     /translation="MSGTLLDRFLDGCTAGEMASGAPDPRALAPAMLLPLRVSRWRDA
                     AALARSIGLRFSALWASERVDGLEVFSVLEADGRHIVLRTMVPDGQELYSLAPVYPAA
                     DRPERHAHDLLGLQLVQQPDSRRWLRHAAWSAQEYPLLRAFPAQRDPGPVPPDDRYPF
                     AVAQGAGVVEIPVGPVHAGIIEPGHFRFLAVGETVLNLEARLGYVHKGIEKIAVGRDP
                     AGLVRLAGRVSGDSTVAHAWAACMALERAAEIELPQRADYLRAVMLERERVANHIGDI
                     GAICMDVGFSFGQYQLSRMREEWQRLSGKVYGHRLMMDQVVPGGVATDLSAELAAALA
                     VQARAVAGEFRGIIERLEASESLEDRLMTTGRLAPELAAELGAIGYVGKASGQAYDVR
                     ARSPHGPYERFPITVPVYRAGDVAARARVRAEETLASLALIEQLLDALPDGPVLAPWR
                     ATAGAGEGVGMVEGWRGELISYVALDVRGRIARYYPRDPSLLNWPALELLVQGNIVPD
                     FPVCNKSINGSYSGQDL"
     gene            10237..10761
                     /gene="nuoB"
                     /locus_tag="PLS34_03435"
     CDS             10237..10761
                     /gene="nuoB"
                     /locus_tag="PLS34_03435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRP86557.1"
                     /translation="MLRMFAKILKVGVVTESPPSLRDSDTAAFEADGKALRAIVDRRF
                     RGSVAIRQVDAGSCNGCELEIQALNNPYHSIERFGVHFVASPRHADILLVTGPVSRHM
                     EAALRRTWAATPQPRWVIAAGDCASCGGEFGVSYASCGAVDKVVPVDLQIPGCPPTPG
                     QLLAGILALLQKRA"
     gene            complement(10803..12518)
                     /locus_tag="PLS34_03440"
     CDS             complement(10803..12518)
                     /locus_tag="PLS34_03440"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HRP86558.1"
                     /translation="MNNAGTRITLTLGLLALVVGSAPARAETYLVEPITDTAASSAEQ
                     GFSQAELDQMLAPIALYPDALLSQVLIAATYPLEIVEAARWSRQNPQLSGEEAVAAVA
                     EQGWDPSVTSLVAFPDLLARMDEDLEWTRKLGDAMLYQETQVMDSIQFLRARADATGT
                     LESTEHVKVIREEKTIVIEPAQTRIVHVPYYDPYVVYGTWWWPAYRPVVWAPPAYYYG
                     YGGYPGFYWGSGIHISTGFFYSSFYWPQRSVVIINTPRYYYPPRYWNSRPYYKHYRNY
                     AYTPGQKWKHNPVHRRGVAYRQPEVRDRYVRYSPGSKSVSTGNFSGTQRPSRDGAAHN
                     RGSRDERNAGNNNREWTRDNQRNAARGQVALPEDRSRTERTEQRFTQRDTAPRAGAGA
                     PAANTRPQQGARPDAGSLERRLGTTRGTGESTSRPTVAGATSRPSAQAVRPQVTQRAT
                     VPARAPLATRPQATQRSTVPTQTPQASRSQATQRTTAPSRAPQAARQPEAPRATAPAP
                     SRQASPPPAAPRLPSAPSASSRSEPAPAAAPSRSSAPRAAPERRSASGSSQSRAHGGS
                     RDSRR"
     gene            12703..12793
                     /gene="ffs"
                     /locus_tag="PLS34_03445"
     ncRNA           12703..12793
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS34_03445"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(12819..13055)
                     /locus_tag="PLS34_03450"
     CDS             complement(12819..13055)
                     /locus_tag="PLS34_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP86559.1"
                     /translation="MKHIQILGSGCSRCQVLADNAEAAAKSLGLEYTLEKITDVNRIA
                     EFGIMLTPALVVEGEVKTFGRVLSPENIRPLLEG"
     gene            complement(13100..14482)
                     /locus_tag="PLS34_03455"
     CDS             complement(13100..14482)
                     /locus_tag="PLS34_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRP86560.1"
                     /translation="MEAVHEAPILRREWRLLVAGVAMFVLVFTLPIGWPRFDGAVHEA
                     LALTRWYAQEHFLLCLVPAFLIAGAIAVFISQAAVMKYLGPAAPKPVAYGMGAVSGSI
                     LAVCSCTILPLFGSIWKRGAGLGPAIAFLYSGPAINVLAIVLTASVLGAELGMARAIG
                     AIGFSVLIGLAMHVIYRGEERARAGGALLIPEADETRPLWQTASYFAALIGVLVFANW
                     GRPEASSGPWFGIWNAKWALTGLFGALLGVMMVLWFGIRWWKMALLALAVALTAVLLR
                     VWALPTWGHLLPGFENGLPWHTMLPFTVAVAGIVLLCSGRDAGDQNREWVEQSWGFAK
                     QITPLLLIGVLVAGFLLGRPGEEGLIPGHWIGDLVGGNSLAANFIAALAGSVMYFATL
                     TEVPILQGLIGNGMGKGPALALLLAGPAVSLPNMLVIRAILGTQKTLVFVGLVVALST
                     LCGFLYGAFL"
     gene            complement(14484..14822)
                     /locus_tag="PLS34_03460"
     CDS             complement(14484..14822)
                     /locus_tag="PLS34_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRP86561.1"
                     /translation="MKARQTQPAWQRFELQADVLQGLAHPIRLAIADLLRDGEVCVCD
                     IAAAVGAERSNVSRHLAVMARSGIVGTRKESLKVFYRLRTPCVLDFMACATRTIEHDL
                     AERTRALADG"
BASE COUNT         2248 a         4875 c         5133 g         2711 t
ORIGIN      
        1 ggacaggtta gggccgaggc tgagcgcagc ctgaaactgc cggccgtgca ggtgctcggc
       61 gaacacgccg cgcgtctccc cgagtccggg gtcgatgcct ttgtgcccgc ggcgggcggc
      121 ggcttccggg agattcgcta cgaggagcgc gatgcggtcg ggtggctgca tttcgacttt
      181 cacaacggcg ccatgaacac ggcgcagtgc cgacgcctgc tggcggcctg gcgccatgcg
      241 gccgcgcgtc cgacgcgcgt gatcgtcctg gccgggggac gggacttctg gtccaacggg
      301 ctgaatctca acgtcatcga ggccgccgac agcccggcgg acgcatcctg ggacaacatc
      361 aacgcgatcg acgacctcgc cgaggccatc gtgcgcgaga ccggcaagct cacgctcagc
      421 atggtgggcg ggaacgcggg cgccggcgga gtatttctcg ccctcgcggc ggaccacgtg
      481 ttgctgcgcg acggcgtcgt cctcaacccg cattacagga acatgggcaa cctgtacggc
      541 tcggagctct ggacctacct gctgccgcgc cgggtgggcg agtccggggt cagggcggtc
      601 atggatcacc gtctgcccat gctggcgcgg accgccgtag cgagcggcct ggcggatgaa
      661 ctcgggcccg gcgaggtcga ggcgttcgcc gcgcacgcca aggcacgcgc cctgacgctg
      721 gcggcactgc cggactggga acggcggctc gccgacaagc aacggcgtcg cgcggccgac
      781 gaagccgaac ggccgctcag cgactatcgc gcggccgagc tcgagcggat gaaacggaac
      841 ttctatggat tcgatccgag ttaccacgtg gcacgattcc gcttcgtgca tcgcaacccg
      901 cacgcctgga cgccgctgta ccttgcggcg catcgccgcc tgcgcggacc gtcaccccgg
      961 cactgacgga gcccgctcgg ctcgagcgcc gggcgggcgc gagcccggcc ggcacattcg
     1021 ggtatcattc cgcgccacac aggttggccg agcggagcct cgagatgcgg aaaattgttg
     1081 cagttgcatt cgtgctggcg ctgtcgctgg cggcgccggc aggtgccaaa cccctcgatg
     1141 cggggacgct ctggcagctc cagcggctgg ccagcccggc gatctcgccg gacggcaagc
     1201 tggcggtggt ggcggtgacc cgctacgagg tgccggagga caagggcatc accaacctct
     1261 ggctggtacc gaccgacggc ggcgcggcgc ggccattgac gacgcatccg ggcagtgact
     1321 ccagcccggc ctggagtccg gacggccgcc acatcgcctt cgtctcgcgg cgtggcgacg
     1381 acgagcagag ccagctgtac gtcataccgc tgggcggcgg cgaagcgcgg cgcgtgacca
     1441 aggtgccgac cggcgccagc gcccccaagt ggttcccgga ctcgaccgag ctggcgttca
     1501 tcagccgcgt ctacgaggat gccgatggct gggacgacat ggtggcccga ctgaaggcgg
     1561 agaaggactc caaggtctcc gccaggacct gggacacggc gccgctgagt ttctgggacc
     1621 gctggcacga tgcgcgccgg ccgcacgtct accgcgtctc ggtcgacggc ggggagccgc
     1681 gaccggtgac cctcggctcg ggggttcacc tgctgatcag ctcggccgac gccgccagtt
     1741 atgacgtttc ccccgatggc gccgagcttg cggtggttgc ggacagcgat gccagcggcg
     1801 tcgagagcaa tcccgatatc tacctggtgc cggccgcggg cggcgcggcg cgcaacgtca
     1861 cgccggacag cccggcgccc gacttcgcgc ccgtctacag cccggacggc cgctggctgg
     1921 ccttctcgca gcagcgcatc cccggcttct acggggaccg cgcgcggctg gtgctgcacg
     1981 atcgccgcga cggggcgcat cgcgtggtca cggaagattt cgaccgctcg gtcagtggcg
     2041 tgctctgggc gcctgacagc cgcaacgtct atgcagccat cgacgatgcg gcgcacgagc
     2101 ggatctatgc catcgacacc cgcaacggcg ccacgcgcgc gatcacccgg gagcacagct
     2161 tcggcagccc ggcgatgtcg caagacggcc gcgtcctggt ggcgctgcgc cagagtttca
     2221 tagagccgcc gaccctggtg cgcgtcgagc cacggcgcgg cgacgtcacc aagttgtcga
     2281 gtttcaacga cgagctgctg gctggcgtgg acttcggccg ctacgagagc gtcacctacg
     2341 aaggcgccaa cggggcgccg atccagatgt gggtcgtcta tccgcccggc ttcgacccgg
     2401 agaagaagta cccgctctac ctgctgctgc acggcgggcc gcacaacggc attccggacg
     2461 gtttccattt ccgctggaat gcgcaggtgt tctccggctg gggttacgtg acggcctggc
     2521 acaacttcca cgggtcaagc ggcttcggcc aggacttcac cgatgccatc aacccgaacc
     2581 agtccgagct gccctacgtc gacaccatca aggctgcgga atggttcgcg cgcaagccat
     2641 ggatcgacgc cgagcgcatg gcggccggcg gcggcagcta cgggggctac ctcgcttcga
     2701 tcctgctggg tcgcgatcat cccttcagga cgctgatcgc ccacgcggcg gtgttcaact
     2761 ggaagacgca gtacgccgcc gactacggcg cgtcgaggcg ccgtcacggg gaatactggg
     2821 tcaatcccga gcactacgag ttgtcctcgc cccacatggg ggcggccaac ttcgacacgc
     2881 cgacgctggt gatccacggc cagctcgatt accgggtgcc gctgaaccac ggtatcgagc
     2941 tgttcaacat cctgcagaac cgcggcgtgc gcagccgctt cgtctactat ccggacgaaa
     3001 accactggat cctcaagccg aacaactccc tgcactggta ccgcgaaaag caggattggc
     3061 tggcggagtt tcttcgctag ctgcgtgagc ggtgccgggt cgaggcgcgt tcctggtccg
     3121 ccagagggct cggcagaggc cctcgtggcg agcaggcccg gcttgccttg gtgatccata
     3181 agggatcaga aagtagttct ggaggttgaa aaataccgac tcagggcata ttcttgatgc
     3241 attgccgcat catgatgact gacctggacg gatgctcact gccgcgcaac tacgtgccgc
     3301 ccgagccttg ctcgacctga gccagcgcca gctggccgag gccgctggcg tttccgtgcc
     3361 cacgatccgg cggatggagg ccagcgccgg cactgtgcgc gccaacgtcg acactctcgt
     3421 caagatcatc gatgcactgg atgtcctcgg ggtcgagttg ctcggcgagg ggaccccgag
     3481 cctgggcggc ggtcgcggga tccgcctcaa gcaatgactg caaccgacat gctgccgatg
     3541 ttcgtggggc tggctctggg cggatccctg cttgctgctg tcctggggct cttcacgggt
     3601 cgcaagcgcc cggccatgca ggtgttgttc tgcgccagcc tcggtgcggg cggcctggcc
     3661 gggaccgctg cgggcgccct gatgttgtct ggggccggcg gcttcggtgt cgagttgccg
     3721 gtcgggttgc cctggttgcg gttgcacctg caactcgacg cgctcggcgg cacgttcctc
     3781 ggcctaatcg gcatggtcac gatcgccgtc agcatgttcg cgccgcgcta cacgcgggaa
     3841 taccagcgac ggcgccattc gcccggtgcg ttgggcctgg ccaccggact gttcgttgcg
     3901 ggcatgcagt tcgtggtgct tgccggggac gcattcggat tcatgatcgc ttgggaattg
     3961 atgtcggtgg ccagttacct gctggttgcc tacgagcacg aagaaagcgc gcatcgcagg
     4021 gcggctttca tttacctgct gatggcacaa atcggcgccc tgctcatcct gcttgccttt
     4081 ggcgtattga gtggattcga gggcgacttc acgttcgatg cgctgcgcga gaaagccctc
     4141 gaaccaccgt ggccggggat cgcgttcgcg ttggcgctgc tggggttcgg tatgaaggct
     4201 ggtcttgtgc cgctccatgt ctggctgccg gaggcgcatc cggcagcgcc ggcgcacatc
     4261 tcggccctga tgtccggcgt gatgctgaag gtggcgatct acggcttcat tcgcttcagc
     4321 ttcgacctgc tcggcgcacc ccactggagc tggggcgtgg ccgtgctgct ggccggcagc
     4381 atcacggcgc tgtacggcgt gctgtacgcc attatgcagc aggagctgaa gcgcgtcctc
     4441 gcatactcct ccatcgaaaa tgtcggcgta atcttcatcg gcctgggcct ggcgatcact
     4501 ttcatcggca caggccaccc ggcgcttgcc gcccttgggc tggtcgctgc cctgtatcac
     4561 tgcgtgagtc acgccctctt caagggcctg cttttccttg gcgccggcgc ggtggtgcag
     4621 gcgagccaca ccgggacgct cgaccagatg ggtgggctga tccgggtaat gccatggaca
     4681 gcgttgttct ttctcgtggg ttgcctgggc agcgcggccc tgccgccgtt caatggcttc
     4741 gtttccgagt ggctgatttt tcagacggca ctgcaggcca ccgtgctcga tgatggggtg
     4801 ctcagggccg tcatacccgt gacggccgca atgctcgctt tgacgggcgc gttggcggct
     4861 gcctgcttcg tgcgcgcctt cgggatcgcc ttcctcggcc aggggcggac ccgtcgcgtg
     4921 cgtcatgcac gcgaggtttc ccgtggcatg atcgccgggc aggggctgct tgcgctgctt
     4981 tgcctgctgt tcggaattct ccccaccttc gcggtggcgg gcttggggcg cgtcgcggaa
     5041 ggcctcaccg gctatgcgct gtcatccgcg accgaccgcg gctggctctg gctgacgccg
     5101 gtcgcgcccg aggtcgcgag ctacagcgcg ccgctggtat tcctcggggt ggggggcgcg
     5161 ctggccgttt gggcaattgc gtacctggcg gtacggcggc agcgccacgg cgcacccatg
     5221 ccgcgaagcg acgcctggga ttgcggtttc ggcggtacca gtgcacgggc gcagtacacc
     5281 gcaacggcat tttccatgcc gatccggcag gtgttcgaac ccgttttcga gctgcaggag
     5341 cagacggttc gcgagatgca gcccgaactg gtcacgcgtc cggcgcacat ccgttacctg
     5401 ctgcacgttg aagatcgcgc ctggaagatc ttctaccggc cggtcgagcg ggcggtccag
     5461 cgcgctgcac ggctgatcgg cagaatccag accgggcagt tgcgacacta cctgatctat
     5521 tccttcgtca ccctgttgct gctgctatgg ctgatcacct gaatccggtt gcctggctgc
     5581 tggcgctgct gcagaccctg atgttcgtgc ttgctgcgcc ggtttttgct gcctgggtga
     5641 agcgggtgaa atgccggctg caaaaccgtc gcgccccgtc actgctgcag ccgtggcgtg
     5701 acatccgccg gctgttcgcg aagcggctgg tactcgcgca cactgcgtcg ccggtgttcc
     5761 gggtggcgcc gtacattgtg tgcggcacga ctatcatggc ggcgtcggtg gtgccgctgg
     5821 tggcgttgac cctgccgctg gcggctatgg cggacgcgat cgtgctggtg gcgctgatcg
     5881 ccttgggccg cttcttcctg gcgctcgccg gcctcgacgt ggggactgca ttcggcggca
     5941 tggggtcctc gcgcgagatg accgtctctg cgttggccga gcccgcaatg ctgatggtgg
     6001 tgttcacgct ggcaatgatt gcatcgagca ccaatctcac cacagtcgtg acgcacatgc
     6061 tggtcgaggc gcaggcactg cgtccttccc tgctatttgc gctcatcgca ctgatccttg
     6121 tcgggatcgc agaaaccggg cgaattccgg tggacaaccc ggcgacgcac ctcgagctca
     6181 ccatgatcca cgaggcgatg gtgctggagt attcgggccg gcacctggcg atgatggacc
     6241 tggccgcgca gctcaagctg atgatctatg cagtgctggt agccaacctg ttttttccgt
     6301 gggggatcgc gatcgagaac acccctgcag cctggttaat cgccacgggc ctgctgctct
     6361 gcaagctgac gctgctcggc atcggcctgg cagtggcgga aacggcggtt gccaagatgc
     6421 gcctgttcaa ggtgccgacc ttcctcgccg ccgccttcat tattgcgctg gtcgggatgc
     6481 tcagtgccct gatcctggag ggcggcgcat gactccctac ctcgagttgc cgctcgtcga
     6541 acaggccatt ctcacagtcg ccgcactggt gctggtgagc agttttgcgc tgctggcgca
     6601 gtcgcgcgtg gtggctacca tccatatatt tgcatggcag ggcgggttgc tggccaccgg
     6661 ctgcgcgctg gtggcgtgga gcaccggcga aacgcacctg tacctgtcgg ccgggctcac
     6721 gttgttgctc aagtccgcct tcattccctg gctgctggtg cggcaggccc gacgactcgg
     6781 catcctcaag gagttcgacg ccgtgatccg gccgggtgtc accctgttgg ccggcggcgc
     6841 catggtgatt ttctgctatg gcgtggccca gcccctcggc gcgtatgccg agggcacgac
     6901 ccgagatgca atcgcgctca gccttgcggt cgtgatgttg tcgatgctga tgctcgtgac
     6961 gcggcgtaag gcgctcactc aagtcgtggc cttcatggca ctggaaaacg gcatgttctt
     7021 cgcggcgatc accgccacgc gtggcatgcc gatgatcgtg gagttaggca ttgccttcga
     7081 cgtgctggtc gcggccatca ttttcgggat tttcttcttc cacatccgcg acagcatcga
     7141 gtcgctggac atcgagcagc tcaaccggct gtccgagaaa gagccatgag ccccctcctg
     7201 gccactctgc tggcacctgc actggcggcg ctgttgctgg cgttcctcag ttctccccgc
     7261 acgggcgcgt tgctcaacat cgccgcgtcg tcctgcgggc tggcggccgc gctctggctg
     7321 gcggtcgaag tattgcggct cggtgcgatc ggtggtgagc agttctacgt tgacaccttc
     7381 aacgtctacc tgattgtcct caccgcgttc atcggcctga cgacgacgtt tttctcgcgt
     7441 ccctacatgc ggcacgagct cgagatcggc cgcgtcacgg ggcgccggtt gcggctctac
     7501 cacgcgagtt tccagggctt cctgttcagc atgctgttag cgctgtcgac caacaatctc
     7561 ggcgtgctgt gggtggcgct ggaagcggcg acgctggcta cggtgttgct cgtcagcctg
     7621 taccggaccc cggaatcggt ggaagcggcc tggaagtatt tcattctatg cggagtggga
     7681 attgcgcagg ccctgttcgg cacggtcctg ctgtaccttg cggccgcacg cgtcatcgag
     7741 gaccctgacc aggcgttgct ctggagcgtg ctccatgcgc atgcggacca gctcgagccg
     7801 gtggtgatga gtatcgcctt cattttcctg ctggtgggct atggcacgaa ggtaggcctg
     7861 gtgccgctgc acaactggct gccggacgcg cattccgagg gcccgacgcc gatgtctgcg
     7921 gtgctctcgg gcctgctgct caacgtggcc ctgtatgccg tcgtgcgcgt caagatgctc
     7981 accgatgcgg ccctcggcgc ggacgccggt gagccggggc tggccggctt cctgatgatg
     8041 ggattcgggc ttgcctcatt catcgtcgcc ggcctgttcc tgcatcgcca gcgcgacatc
     8101 aagcggctgt acagctactc gtccatcgag cacatgggcc tgatgacttt cgctttcggg
     8161 cttggcggcg cactcgccac atttggagcc ctgctgcaca tgaccgtgca ctccctggcc
     8221 aagtcggcca ttttcgtcac cgtcggtcat gccagccaga tcgccggcac ccagtccatc
     8281 gatgccatcc gtggcttgat ccggacacag cccgcggttg gctggggcct gctggtcgga
     8341 tccctggcga tcgccggctt tccgcccttc ggcgtgttcg tcagcgaatt cctggtgctg
     8401 acggcgacca tgcaggccta tccctggttg acggtgccgc tgctggtggg cctagccgtg
     8461 gcgtttgccg ggctgttccg gcaccttcag ccgatggtct acggcccatg cccgcccggg
     8521 caagagccca tccgggtgag catggcgccg gtgatcctgc acctggccat cgttttgtgg
     8581 ctcggcctcg cgattccggc attcctgtcg cgctggttcg acgaggcgac acggcttatc
     8641 tcggggagcg gattgctgtg agcgggaccc tcctggaccg gtttctggac ggttgcacgg
     8701 ccggggagat ggcctccggg gcgcccgatc ccagggcgct tgcgccggcg atgctcctgc
     8761 cactgcgtgt ttcccggtgg cgggatgccg cggccctggc ccgctccata gggctcaggt
     8821 tcagcgcgct gtgggcgagc gagcgagtgg acggcctcga ggtgttcagc gtgctggagg
     8881 cggacggccg tcacatcgtg ctccgcacaa tggtgcccga cgggcaagag ctctactcgc
     8941 tggcgccggt gtacccggcg gcggaccggc cggaacggca tgcacacgac ctgctcggcc
     9001 tgcagttggt gcagcagccc gacagtcgcc gctggctgcg ccatgccgcg tggtccgcac
     9061 aggagtaccc gttgctgcgg gcgttcccgg cgcagcgcga cccgggcccg gtgccgccgg
     9121 acgatcgtta tccattcgct gttgcgcaag gcgccggcgt ggtcgagatc ccggttggac
     9181 cagtgcatgc gggcatcatc gagcccgggc acttccgctt tcttgcggtc ggcgaaaccg
     9241 tgctgaacct cgaggcgagg ctggggtatg tccacaaggg aatagagaag attgcggtcg
     9301 gtcgcgaccc ggccggcctg gtgcggctgg ccggccgcgt atcgggcgac tccacggtgg
     9361 cgcatgcctg ggccgcatgc atggcgctgg agcgggccgc ggaaatcgag ctgccgcagc
     9421 gggccgatta cctgagggcc gtcatgctgg agcgcgagcg tgtcgccaat cacatcggtg
     9481 acattggcgc catctgcatg gacgtgggtt tcagtttcgg ccagtaccag ctgtcgcgca
     9541 tgcgcgagga atggcaacgg ctcagtggca aggtctacgg ccaccgactg atgatggatc
     9601 aggtcgttcc cggcggcgtg gcaaccgacc tgtcggcgga acttgcggct gcgttggccg
     9661 tccaggcccg ggcggtcgcc ggtgagttcc gcggcattat cgaacgactc gaggcgtccg
     9721 aatcactgga agaccggttg atgacgaccg gacggcttgc gccggagctg gctgctgagt
     9781 tgggcgcaat cggttatgtc ggcaaggcct cgggccaggc ctatgacgtg cgagcgcgct
     9841 cgccacacgg tccctatgag cgcttcccga tcaccgtgcc ggtgtatcgc gcaggcgacg
     9901 tcgccgcccg agcgcgggta cgtgccgagg aaaccctggc atcgctggcc ctcatcgagc
     9961 agctgctcga tgcgctgccg gacggtccgg tgctggcccc atggcgtgcc acggccggcg
    10021 caggcgaggg cgtgggcatg gtcgagggct ggcgcggcga gctgatcagc tacgtggcgc
    10081 tcgacgtgcg ggggcggata gcgcggtact acccccggga tcccagtctg ttgaactggc
    10141 cggccctcga gctgctggtc caggggaaca tcgttccgga ctttccggta tgcaacaagt
    10201 cgatcaacgg ctcgtactcc gggcaggatc tctagcatgt tgagaatgtt cgccaagatt
    10261 ctgaaagttg gcgtcgtgac ggagtcgccc ccctcgttgc gcgattccga cacagcggct
    10321 ttcgaggccg acgggaaagc actcagggca atcgtggatc ggcggttccg cggcagcgtg
    10381 gcgattcgcc aggtcgatgc cggctcatgt aacggctgcg agctcgagat ccaggcgctc
    10441 aacaatccct atcactcgat cgagcgcttc ggtgtgcatt tcgtggcatc gccccgccac
    10501 gccgacatcc tgctggtcac cggcccggtg tcgcgccaca tggaggccgc gttgcgccgt
    10561 acatgggccg cgaccccgca gccccggtgg gtcatcgcgg cgggcgactg tgcgagttgt
    10621 ggcggcgagt tcggggtcag ctatgcaagc tgtggcgcgg tcgacaaggt ggtcccggtg
    10681 gacctgcaaa ttccaggttg cccgccaacc cccggccaac tgctggccgg tatcctggcg
    10741 ctgctgcaga agcgcgcctg agggcgccgt acccgcgcgc cgccaggagt cgaggcctga
    10801 gcctagcgcc ggctgtcgcg cgagcctcca tgggcgcgcg actggctgct gccggaggcc
    10861 gaacgtcgct caggcgccgc acgtggcgcg gacgacctcg acggtgcagc tgccggcgca
    10921 ggctcgctgc gggaggaggc actcggtgca gaaggtagac gcggcgccgc cggaggcgga
    10981 ctggcctggc gggacggcgc aggcgctgtg gcccttggcg cctcgggctg gcgcgccgcc
    11041 tgtggagcgc gactcggcgc ggtggtgcgc tgggtcgcct gggaacgcga cgcctgaggt
    11101 gtctgagtcg gtaccgtgga ccgctgggtc gcctggggcc gtgttgccag gggcgcccgg
    11161 gcaggcaccg tggcgcgctg ggtcacctgc ggccgcaccg cctgcgcact tggccggctg
    11221 gtcgcaccgg ccaccgtcgg acgcgaagta ctttcaccag tgccccgcgt cgtgcccagg
    11281 cggcgctcga gactgcccgc atcgggtcgc gcaccctgct gcggccgggt attcgcagca
    11341 ggcgctcctg cgcctgcgcg cggcgcggtg tcgcgctgag taaagcgttg ctccgtgcgc
    11401 tcggttcggc tgcggtcctc cggcagcgcc acctggccgc gcgcagcatt gcgctggttg
    11461 tcgcgggtcc actcccggtt gttgttgccg gcgttgcgtt catcccggct gccgcgattg
    11521 tgagcggcgc cgtctcgcga cggccgctgc gtgccgctga aattcccggt ggacacggac
    11581 ttcgagcccg ggctgtagcg cacgtagcga tcgcggacct cgggctggcg ataggccacg
    11641 ccgcggcgat gcaccgggtt gtgtttccac ttctgtcccg gcgtataggc gtaattccgg
    11701 tagtgcttgt aatacgggcg tgaattccag tagcgcggcg gatagtaata acgcggcgtg
    11761 ttgatgatca cgacgctgcg ctgcggccag taaaagctgc tgtagaagaa tcctgtggag
    11821 atgtggatgc cgctgcccca gtagaaaccg ggatagccgc cgtagccgta atagtaggcc
    11881 gggggcgccc aaacgaccgg gcgataggcg ggccaccacc aggtgccgta caccacgtag
    11941 ggatcatagt agggcacatg cacgatgcgc gtctgagccg gctcgatgac gatggtcttc
    12001 tcttcgcgaa tgaccttcac gtgctcggtc gactcgagcg taccggtggc gtcggcgcgc
    12061 gcacgcagga actggatgga atccatcacc tgcgtctcct ggtacaacat ggcgtcgccc
    12121 agcttgcggg tccactcgag gtcttcgtcc atgcgtgcca gcaggtccgg gaaggcgacc
    12181 agcgaggtca cgctcggatc ccatccctgc tcggccacgg ccgccaccgc ttcctcaccg
    12241 gacagctgcg ggttctggcg cgaccagcgc gccgcctcga cgatctcgag cgggtaggtg
    12301 gcggcgatca gcacctgcga cagcagggcg tccgggtata gggcgatcgg cgcgagcatc
    12361 tggtctagct cggcctgcga gaagccctgc tcggccgacg acgcggccgt atcggtaatg
    12421 ggttcgacca ggtaggtctc cgcccgggcc ggggcgctcc ccacgaccag ggccagcagc
    12481 cccagcgtca gcgtaatgcg agtgccagcg ttgttcatgg ttcgctcccg gttttcacca
    12541 cggtctcagc ctaccaccgc caacctgaac gtacggcaac ggtgttctgt tgccggcagc
    12601 cccctgtatg gccgatattg attatgactc tttcaagccg tggggagttt ccggttaaca
    12661 tagcgcggcc cggatttccc gggttccgtc catggtggtc cgcgtcggtc ccctcgcgac
    12721 ggccagccgt gaaccccgcc aggcccggaa gggagcaacg gtagcggcga cgccgggcgc
    12781 cggggtgtgg ctggcgcggg ccgccaccct cttgccgctc agccctcgag cagcgggcgg
    12841 atattttccg ggcttagcac ccgtccgaag gtcttgacct cgccctcaac gacgagggct
    12901 ggcgtcagca tgatgccgaa ctcggcgatg cggttgacgt ccgtgatctt ttccagggtg
    12961 tattcgaggc cgagcgattt ggcggccgcc tccgcattgt ctgccagcac ctggcacctg
    13021 gagcagccgc taccaagtat ttgtatatgt ttcatatctt tgtgccttga aattaatgtg
    13081 acttattgtc gcgccggggt tacaagaacg cgccgtacag gaagccgcac agggtactca
    13141 gggcgaccac caggccgacg aagaccagcg tcttctgcgt gccgaggatc gcccggatga
    13201 ccagcatgtt cggcagcgag accgccggtc cggcgagcaa cagggccagc gcagggccct
    13261 tgcccatgcc gttgccgatc agtccctgca ggatgggtac ttcggtcagc gtggcgaaat
    13321 acatcacgga gcccgccagg gccgcgatga aattggcggc cagcgagttg ccgccgacca
    13381 ggtcgccgat ccagtggccg gggatcagtc cttcctcgcc gggcctgccc agcaggaagc
    13441 cggccaccag cacgccgatg agcagcaacg gggtgatctg cttggcgaag ccccagcttt
    13501 gctcgaccca ctcgcggttc tggtcgccgg cgtcgcggcc ggagcacagc agcacgatgc
    13561 cggccaccgc gacggtgaac ggcagcatgg tgtgccatgg caggccgttt tcaaatccgg
    13621 gcaacaggtg gccccaggtc ggcagcgccc agacgcgcaa caggacggcc gtgagcgcaa
    13681 cagcgagcgc cagcagcgcc atcttccacc agcggatgcc gaaccacagc accatcatga
    13741 cgcccagcag ggcgccgaac agcccggtca gcgcccactt ggcgttccag atcccgaacc
    13801 acggccccga gctggcttcc ggccggcccc agttggcgaa caccagcaca ccgatgaggg
    13861 cggcgaagta actggcggtc tgccacagcg gtcgcgtctc gtccgcctcg gggatcagca
    13921 gggcgccgcc ggcgcgggcg cgctcctcgc cccgatagat cacgtgcatg gccaagccga
    13981 tcagcacact gaagccgatg gcgccgatgg ctcgcgccat gccgagttct gcgcccagca
    14041 ccgaggccgt gagcacgatt gcaagcacgt tgatggccgg gccggaataa aggaacgcga
    14101 tcgcagggcc gaggcccgcg ccgcgcttcc agatgctgcc gaacagcggc aggatcgtgc
    14161 aggagcaaac tgcgaggatc gagccgctca cggcgcccat gccgtaggcg accggcttcg
    14221 gcgccgccgg acccaggtat ttcatgaccg ccgcctggct gatgaacact gcgatcgcgc
    14281 cggcgatcag gaacgcgggc accaggcaga gcaggaaatg ctcctgggcg taccagcgcg
    14341 tcagcgccag cgcctcgtgg accgcgccgt cgaagcgcgg ccagccgatc ggcagcgtga
    14401 acaccagcac gaacatcgcc acaccggcga cgagcaggcg ccattcgcgg cgcaggatcg
    14461 gggcctcgtg aactgcttcc atgtcagcca tccgccaggg cgcgggttcg ctcagcaagg
    14521 tcgtgttcga tcgtgcgagt ggcgcaggcc atgaagtcca ggacgcatgg cgtgcgcagg
    14581 cgatagaaca ccttcagact ctccttgcgg gtgccgacga tgccgctccg ggccatcacc
    14641 gccaggtggc gcgagacatt ggagcgctcg gcgccgacgg ctgcggcgat gtcgcaaacg
    14701 cagacctcgc cgtcgcgcag gaggtcggcg atggcaagac ggatggggtg cgcgaggccc
    14761 tggaggacgt cggcctgcag ttcgaagcgc tgccaggcag gctgggtttg acgtgctttc
    14821 ataatgtgag tatttactca cctatacatc aaggctattg gggtttgcca cgatgcctcg
    14881 tgagagtgaa tccgggtgct gtgccggcgc cgggacggcc cggctggggt gttacacttc
    14941 cgtcccttcg gaactgaacc cggttga
//
LOCUS       DAOMPG010000039        14285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1832_length_14285_cov_4.721082, whole genome shotgun sequence.
ACCESSION   DAOMPG010000039 DAOMPG010000000
VERSION     DAOMPG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 14285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14285
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_14285_cov_4.721082"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLS34_03465"
     CDS             <1..347
                     /locus_tag="PLS34_03465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRP86562.1"
                     /translation="LLFLSGLGPRAPGGGPIPGVTLDEAGNVAGHDIEIQCRAVFANV
                     RSVLAAAGSSWERLLDVTVFLTDMQRDFATFNRLWAEHFPDDPPCRTTVEVTRLPTPI
                     AIELKCIATVED"
     gene            349..861
                     /locus_tag="PLS34_03470"
     CDS             349..861
                     /locus_tag="PLS34_03470"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRP86563.1"
                     /translation="MKPLRAFNFRQWLDDHRDRLKPPVCNQVVFEEAEFIVMVVGGPN
                     SRTDYHWDPGEELFYQLEGDMLLKTVQHGRVVDLPIRAGEMLLLPPEVPHSPQRFANT
                     VGIVVERRRRPDERDGFMWFCEQCGHKLYEEYLHVSDIVAQLPPIFDRFYGNPAHLRC
                     DACGHQMPPR"
     gene            871..1902
                     /locus_tag="PLS34_03475"
     CDS             871..1902
                     /locus_tag="PLS34_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86564.1"
                     /translation="MSDAPPLKIDVHTHVLPPGWPDLEARYGYGGWVQLAEQGPGCAH
                     LMKDGKFFREIKSNCWDPARRLAECDGHGVHVQVLSTVPIMFSYWAKPADALDLSKLL
                     NEHIAGVAAGAPDRFVGLGTVPMQAPDLAVRELERCVRELGLAGIEIGSHVNGMNLDH
                     PDLFPIFEAARDLGAAVFVHPWEMLGRDRMAKYWLPWLVGMPAETSLSICSMIFGGVF
                     ERLPGLRVLFAHGGGAFPATLGRIEHGFRVRPDLVAVDNDKNPRDYVGRFYVDALVHD
                     PDMLRFMLKLMGAERIALGSDYPFPLGETVPGAMIERMDDLSPATRERLLGGTALEWL
                     GLGAERFRR"
     gene            1899..2741
                     /locus_tag="PLS34_03480"
     CDS             1899..2741
                     /locus_tag="PLS34_03480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRP86565.1"
                     /translation="MIETGQAAGLVLERSGQHYTVDAARGTSIAIPLDVHGPQPAHFG
                     APAARAEPLAGGSFIGDTRRGGSCNCEAVTLVPHCNGTHTEGPGHLTEARLSVHASAL
                     RPLYFAALISVTPEDAAESVETSAPGPQPGDRLITARVIAPALATIGAPPVEALVVRT
                     LPNGAGKRTRDWMKPPMPPYFSREAMALLVRRGVRHLLTDLPSVDRLLDEGQLAGHRV
                     FFGMPPQGRAVADIGRPDATITEMIYVPDALSDGCYALSLQLAAWVSDAVPSRPVLFP
                     LAPA"
     gene            2738..3997
                     /gene="kynU"
                     /locus_tag="PLS34_03485"
     CDS             2738..3997
                     /gene="kynU"
                     /locus_tag="PLS34_03485"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRP86566.1"
                     /translation="MSATDAALLDARDPLAGFRDRFHVPPHGDGDCVYLCGNSLGLQP
                     KSAARYLDEELEAWQLLGVEGHFRGKRPWKDYHEQFAAPLAALTGAHPHEVVCMNGLT
                     VNLHLLMVSFFRPTRSRYKLLIERSAFPSDRYAAVSQLRFHGLEPDDALIEVGRREGE
                     THLRDEDIAAAIRDAGDSLALVLLPGVQYLSGEALDIARLTAEAHAVGALAGWDLAHA
                     IGNLPLALHDAGADFAAWCSYKYLNGGPGAVAGAFVHERHAHESGLPRFAGWWGHDKA
                     ARFRMEPDFVPMPGSEGWQLSNPPVLGMAALLASLEVFVDAGMARLRDKSLALTGLLE
                     QRLEEGLARHVEILTPRDPARRGCQLSLRLRNGAEAGRAVHARLGDAGIVCDWREPDV
                     IRVAPVPLYNRYTDVERFVTALEEALR"
     gene            3994..5352
                     /locus_tag="PLS34_03490"
     CDS             3994..5352
                     /locus_tag="PLS34_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP86567.1"
                     /translation="MSRRISIVGAGLGGALAAIFLARRGLEVTLYERRPDLRRVPQPA
                     GRSINLALANRGLTPLRKAGLGDEVRKLLTVMRGRMIHPVSGALELQPYGRRAHEVIY
                     SVSRPGLNALLLDAAEAAGVRLCFGMRCTAVDFDHDRLEFEHEDSGTRETVALTPCIG
                     ADGAGSVLRKALAVLPGHTASEEILSHGYKELSVLPNGLGRHRMESNALHIWPRGGYM
                     LIALPNHDGTFTVTLFLPLEGEPSFASLDSPAAVRAFFAAEFPDALPLIHDLEAAFFA
                     NPTGMLGTVRCQPWNLDGRVALLGDAAHAIVPFHGQGMNCAFEDCLELDACFDESGGD
                     WPRTFELLSVRRKPNADAIADMALENYVEMRDSVRDPGFLLRQEVAFELERRFPERFA
                     RRYGLVMFRDDVPYAEAQQRGRIQEQILLEITTGIDDAAAVDYDRAGRLIKRRLFPLP
                     GH"
     gene            complement(5378..7198)
                     /locus_tag="PLS34_03495"
     CDS             complement(5378..7198)
                     /locus_tag="PLS34_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86568.1"
                     /translation="MSLFAELRRRNVIRVGGAYAVVAWLLIEVSDTVFPRLGLPEWTV
                     TLVIVLLALGFPVALFMAWAFELTPEGVKRTEHAVAGTAPPGGRVLDWVIALGLLVVI
                     GMFVAERFAVTTPDRAASDPAAVADDGGRPAGSIAVLAFDNMSPDPENAYFAEGISEE
                     ILNILAGVEGLHVASRTSAFSFRGKGVPIPQIAGELNVLHVLEGSVRRQGNRVRITAQ
                     LIRADTDGHLWSQTYDRELVDIFKVQEEIAQAITGALGEILGTRRVSVRAATADIEAY
                     ERFLRGRALFHQRRDLGRAIADLNYVVEQDPEFGEAWVYLAAANFVAPGYDVGSFDQD
                     AMIPAARTGVERARALLPEHPMVIGLQGMLLREEGKPEALELLQRASELSAQDSTPTM
                     WHGLTLFVSGYLEEAETALARAANMDPLVGINHGYLGMVRLALGRDAEAEASLRKAAE
                     LNWPAGLWVHAIELANRGETDRAVALLRELLAQPGPYEASVAAFIVALREPERADSVL
                     DLEGSARPPEEWLALGMTDRYLDLLLDLAVSGRLYNQQGWLRSAWLPSTGALREDPRF
                     FAVAEVLGMVPLWEARGYPKGCRRVVAAAGDRLACTASGP"
     gene            complement(7195..>8404)
                     /locus_tag="PLS34_03500"
     CDS             complement(7195..>8404)
                     /locus_tag="PLS34_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86569.1"
                     /translation="YLRARAFRHDWEEEQIRERIALYRQALEHDLRFALAMGELGREF
                     VNLYWFTTRRDADRAEGRRWLDRALALQPEDPQLRLAMAEYHYRAHLDYEAALKNLDL
                     ATRGLPGSAEAVSLRAYIMRRAGRTAATLDALHSAVLLDPRSGEVIRGLVETYWLTGN
                     MEAALRWQEQLDALPDDHSTNRFWYPNARMQVLGDLEPIRRAFAADTPRTFTLVGAAL
                     GNTFILAYFERDFDKAAAVLDSYEGDLSEDQFGLGIESLSRAYLERARGGEQAAAKAA
                     GGALAVTDRILAEHPDDYRALMARAQALAIRGEGAAAREATRQALSQTIPTRDVVIRS
                     EQRMRELLVLAMVADSTELAAAMDEYLQLEMKYWHFDGLMLDPVFDAHRNHPAIQALA
                     AKYSRRGSGA"
     assembly_gap    8405..8455
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8456..9304)
                     /locus_tag="PLS34_03505"
     CDS             complement(<8456..9304)
                     /locus_tag="PLS34_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86570.1"
                     /translation="MSSSSGLWAELKRRNVFRVAAAYIVVGWLILQVAEIMLGFTGAP
                     EWVGKALIALLLLGLVPALALAWVFEVGPEGVRRDDGSNPRDVSPQARRLDVITLGAV
                     VLVVALLAWEHLGPALSGTRDGSGERQTSAPAVTDPPAAQRVRQEPPPFEAPPGSIAV
                     LPFTNRSAEPDTAYFVDGIHDDLLTQLARNGGLTVISRTSMMEYRDTTKNLRQIGEEL
                     GVATILEGAVQRAGRRVRINAQLIDAASDAHLWADTFDRELTPENVFDIQSEIAAAIS
                     AALGQAL"
     gene            complement(9366..11216)
                     /locus_tag="PLS34_03510"
     CDS             complement(9366..11216)
                     /locus_tag="PLS34_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86571.1"
                     /translation="MSARPGLFAELKRRNVFRVGLFYVVTAWLVIEVAETVLPLFDVP
                     DGVLRGLVVLLVIGLVPALGLSWIYELTPEGIKRDAEMSAADMRGSSTGRKLNWATLA
                     MAVVAIGFMAVERLRPAPPVAAPVVAPAPATVADEAGMVNAASIAVLPFADLSPGADQ
                     EYFSDGMAEEILNALVRVKGLQVASRTSSFGFKGQESLGMPVIAERLQVRHVLEGSVR
                     RAGNTLRITAQLIDAEVDRHLWSDTFDRPLTAENVFAIQEEIASAIVAALVDSLGIEI
                     EDEVRLAQPTENLTAYDMYLRARGLFQGRIDLDVADDLLRQALEQDPEFVNAWELRAA
                     VQTVIVEYGYTRLSRDEHERRGIQFAERALALEPDSGMALATLANLRMGAAAKLLQRH
                     DLGTIIRDLERAVELDPHNSSAHNWLGLSLGLVGRLEDALQTFVRCIEVAPRFGPCRE
                     NHYDILWTLGRPDEAFERLQEALAEGAVVSEYGNFALLAHFEEQTAFMLLANQSKWLP
                     GWHRHRDLYEAFRDLDGDHAELLAALREFQGARPVDAASFTALLLVPLGAFDMLPDYG
                     MLVWGPEYAGFRRSPQFKEYIRGTGVFDYWRDHGYPPMCRPVGEDDFSCD"
     gene            complement(11213..11608)
                     /locus_tag="PLS34_03515"
     CDS             complement(11213..11608)
                     /locus_tag="PLS34_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP86572.1"
                     /translation="MSQVLVVDASVVVDLIGRFRPQPIEALLWAEDSVLAAPELLDVE
                     VLQALRRLDNDGAIPPTRADVIELLRALRIRRYRHYSLRDAIWSLRKNMTAYDAAYVA
                     LARLLDATLVTRDAKLARAPGLDIQVVVP"
     gene            complement(11605..11826)
                     /locus_tag="PLS34_03520"
     CDS             complement(11605..11826)
                     /locus_tag="PLS34_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86573.1"
                     /translation="MIQIRNVPVKIHRALKARAALAGKSMSELILEELQAIMALPSEQ
                     ELRERLQQAEPFAMKKSSAGLIRRERDAA"
     gene            complement(11875..13725)
                     /locus_tag="PLS34_03525"
     CDS             complement(11875..13725)
                     /locus_tag="PLS34_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86574.1"
                     /translation="MSLFAELKRRNVFRVGLFYVVTAWLVIEVAETVLPLFDVPDGVL
                     RGLVVLLVIGLVPALGLSWIYELTTEGIKRDVGPSVAGPQSSDTGRKLNWATLAVAVV
                     AIGFMAVDRMRPAPPVAGPSATPASAAKAPAAVTNMPDNVDAASIAVLPFADLSPGGD
                     QEYFSDGIAEEILNALTRVDGLAVASRTSAFQFKGREIGIPEIAQALAVRHVLEGSVR
                     KAGDTLRITAQLIDASNDRHLWSETFDRSLTAENVFQIQDEIAAAITRALGVAMKLGG
                     EKLVSSAGGTDDIDAYELYLEARTLYHQRKDLDRADALLERALEQDPSFARAWELRAP
                     IPTLAIGYGHSSELLETAEARTREYAARALELNPGSSLALATLGKLLMEKGQYLEGFA
                     DWARALDYFERAIDADANNASAHIWLGNMLESIGLLEEALAAYEQCVTLEPLYLPCVN
                     NRPYVLVALGRFDEAMAMYRRDLGRGLSLYGLHLIPLWVETESDILFMEAANHPEVLQ
                     GFPRLAELYAAHLDPRGDHSAVVTEAVQYLPETPTDLGGQIMDLLLVPLGRNEIHVLG
                     AQLWSDSFRRFRQTEAFRQIIRDTGVLAFWREHRFPQLCRPVGDDDFECD"
     gene            13773..14006
                     /locus_tag="PLS34_03530"
     CDS             13773..14006
                     /locus_tag="PLS34_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRP86575.1"
                     /translation="MKTTLNIDDSVMQRLREAAARQGRTMSELVEAGLRRVLEESEET
                     TNQVREPLPQWQSGGAYVDVADRDALQDRMERD"
     gene            14016..>14285
                     /locus_tag="PLS34_03535"
     CDS             14016..>14285
                     /locus_tag="PLS34_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HRP86576.1"
                     /translation="MFVVDTNVLLYAADVDSEHHAPCRTLIEQARAQAAPWFLTWGIC
                     YEFLRVSTHPRVYRSPWTNAAAWRFIDTLLASPSLSVLAATRRHAA"
BASE COUNT         2332 a         4947 c         4709 g         2246 t
ORIGIN      
        1 gactcctgtt cctgtccggg ctcgggccgc gcgccccggg cggcggcccg atccctggcg
       61 tgacgctcga cgaggcaggc aacgttgccg gccacgacat cgagatccag tgccgggccg
      121 tgttcgccaa cgtgcgcagc gtgctggcgg cggccggatc gagctgggag cggctgctgg
      181 acgtgacggt ctttctcacc gacatgcagc gcgatttcgc gaccttcaat cgcctctggg
      241 ccgagcattt cccggatgat ccgccctgcc gcacgacggt ggaggtgacg cgcctgccga
      301 cgccgatcgc catcgagctc aagtgcatcg cgaccgtgga ggactgacat gaagcccctg
      361 cgtgccttca acttccgcca atggctggat gatcaccgcg accggctcaa gccgccggtg
      421 tgcaaccagg tagtgttcga ggaagccgag ttcatcgtca tggtggtcgg cggcccgaac
      481 agtcgcaccg actatcactg ggacccgggc gaggagctgt tctaccagct cgagggcgac
      541 atgctgctca agaccgtgca gcacggccgg gtggtcgacc tgccgatccg cgcgggcgag
      601 atgctgctgc tgcctccgga agtgccgcat tcgccacagc gcttcgcaaa caccgtcggc
      661 atcgtcgtcg agcgccggcg ccgtccggac gagcgggacg gcttcatgtg gttctgcgaa
      721 cagtgtggcc acaagctcta tgaggaatac ctgcacgtca gcgacatcgt ggcgcagctg
      781 ccgccgatct tcgatcgctt ctacggcaac cccgcccacc tgcgctgcga cgcctgtggc
      841 catcaaatgc cgccgcgcta gcctgccgac atgagcgacg ctccaccgct gaagatcgac
      901 gtccatacgc acgtcctgcc gccgggatgg ccggacctcg aggcgcgtta tggctacggc
      961 ggctgggtgc agctcgccga gcagggcccg ggctgtgccc acctcatgaa ggacggcaag
     1021 ttcttccgcg agatcaagtc gaattgctgg gacccggcgc ggcggctcgc cgagtgtgac
     1081 ggccacggcg tccatgtcca ggtgctgtcg acggtcccga tcatgttcag ttactgggca
     1141 aagccggccg acgctctcga cctctcgaag ctgctcaacg agcacatcgc cggcgtggcc
     1201 gccggcgcgc ccgaccgctt cgtcggcctc ggcacggtgc cgatgcaggc gcccgacctc
     1261 gcggtgcggg agctggagcg ctgcgtgcgc gagctcggtc tcgccggcat cgagatcggc
     1321 tcgcacgtca acggcatgaa cctcgaccac ccggacctgt tcccgatatt cgaggcggcg
     1381 cgggatctcg gcgcggccgt attcgtgcat ccctgggaaa tgctcggccg cgaccgcatg
     1441 gccaagtact ggctgccctg gctggtgggc atgccggcgg agacctcgct gtccatctgc
     1501 tcgatgatct tcggcggggt gttcgagcgc ctgcccgggc tgcgcgtgct gttcgcgcac
     1561 ggcggcggcg ccttccccgc gacgctcggg cgcatcgagc acggctttcg cgtccggcca
     1621 gacctggtcg cggtggacaa cgacaagaac ccgcgcgact acgtcgggcg cttctacgtc
     1681 gacgcgctgg tgcacgatcc cgacatgctg cgcttcatgc tcaagcttat gggcgcggag
     1741 cgcatcgccc tcggcagcga ttaccccttc ccgctcggcg aaaccgtgcc gggcgccatg
     1801 atcgagcgga tggacgatct gtcgccggcc acccgcgagc ggctcctggg cggcacggcg
     1861 ctggagtggc tggggctcgg cgcggagcgg ttcagacgat gatcgaaacc ggccaggctg
     1921 cgggactggt gctggaacgt tccgggcagc actacacggt ggacgccgcc aggggcactt
     1981 cgatcgccat cccgctggac gtccacggcc cgcagccggc ccatttcggg gccccggcgg
     2041 cgcgtgctga gccgctggcc ggcggcagct tcatcggcga cacgcgtcgg ggcggcagct
     2101 gcaactgcga ggccgtcacg ctggtgcccc attgcaacgg cacccacacc gagggacccg
     2161 gccacctgac cgaggcgcgc ctgtcggtgc acgccagcgc gctgcggccg ctgtactttg
     2221 cagcgctgat ctcggtgacg cccgaagatg cagccgaatc ggtcgagacc agcgcgccag
     2281 gaccccagcc tggcgaccgg ctgatcacgg cgcgcgtcat tgcgccggct ttggcgacca
     2341 tcggcgcgcc gccggtcgag gccctggtcg tgcgcacctt gccgaacggc gccggcaagc
     2401 ggacgcgcga ctggatgaag ccgcccatgc cgccgtattt ttcgcgcgag gcgatggcgc
     2461 tgctggtgcg gcgcggggtg cggcacctgc tgaccgacct gccgtcggtg gaccggctgc
     2521 tcgacgaggg ccaactggcc ggccaccggg tgttcttcgg catgccgccg caaggtcgcg
     2581 ccgtcgccga tatcggacgc ccggatgcca ccatcaccga aatgatctat gtccccgatg
     2641 ccctgtcgga cggctgttat gcgctgtcgc tgcagctcgc tgcctgggtg agcgacgcgg
     2701 tgccgtcgcg cccggtgctg tttccgctgg cgcccgcatg agcgcgaccg acgcagcgct
     2761 gctggacgcc cgcgacccgc tggccggctt ccgggatcgc ttccacgtgc cgccgcacgg
     2821 tgacggtgac tgcgtgtatc tctgcggcaa ctcgctgggc ttgcagccaa agagcgcggc
     2881 gcgttacctg gacgaggaac tggaggcctg gcagctgttg ggcgtggaag gccacttccg
     2941 cggcaagcgt ccctggaagg actaccacga gcagttcgcc gcgccgctgg ccgcactcac
     3001 cggcgcgcat ccccatgaag tcgtgtgcat gaacggcctg accgtcaacc tgcacttgct
     3061 gatggtcagc ttcttccggc ccacgcggtc gcgctacaag ctgctcatcg aacgctcggc
     3121 ctttccctcg gatcgctacg ctgccgtctc gcagctgcgc ttccatggcc tggagccgga
     3181 cgacgcgctg atcgaggtgg gccggcgcga gggagagact cacctgcgcg acgaagatat
     3241 tgcggcagcg atccgggacg ccggcgactc gctggcgctg gtgctgttgc cgggtgtcca
     3301 gtacctgtcg ggcgaagcgc tcgacatcgc ccgactcact gccgaggcgc acgccgtcgg
     3361 tgcgctggcc ggctgggacc tggcgcacgc catcggcaac ctgccgctgg cactgcacga
     3421 tgccggcgcg gacttcgccg cctggtgcag ctacaagtac ctgaacggcg ggcccggcgc
     3481 cgtcgcgggc gccttcgtgc acgagcgcca cgcccatgaa tccgggctgc cacgttttgc
     3541 cggctggtgg ggacacgaca aagcggcgcg attccgcatg gaaccggact tcgtgcccat
     3601 gcctggctcc gaaggctggc agctgtcgaa ccctccggtg ctgggcatgg ccgccctgct
     3661 cgcctcgctg gaggtgttcg tggatgcagg catggcgcga ttgcgcgaca agtcgctggc
     3721 actcacgggc ctgctggagc agcgcctgga ggagggcctg gcccggcatg tcgaaatcct
     3781 caccccgcgc gatcccgcgc ggcgcggctg ccagctttca ctgcggctgc gcaacggcgc
     3841 cgaagccggg cgggcggtgc atgcgcgcct cggcgacgcc ggcatcgtct gcgactggcg
     3901 cgagccggac gtcatccggg tcgcgccggt gccattgtac aaccgctaca cggacgtcga
     3961 gcgcttcgtg acggcactcg aggaagcgct gcggtgagca ggcgcatcag catcgtcggc
     4021 gccggcctgg gcggcgccct ggccgccatc ttcctggcgc gccgcggcct cgaggtcacg
     4081 ctctacgagc gccgccccga cctgcggcgc gtgccgcaac cggcgggacg gtccatcaac
     4141 ctggcgctcg ccaaccgggg actgacgcca ctgcgcaaag ccggccttgg cgacgaggtg
     4201 cgcaagctgc tgaccgtgat gcgcgggcgg atgattcacc ccgtgtccgg agcgctcgag
     4261 ctccagccct atggccggcg cgcccacgag gtcatctact cggtgtcgcg tcccggcctg
     4321 aacgccctgt tgctggatgc cgccgaggcg gccggcgtgc gcctgtgctt cggcatgcgc
     4381 tgcacggcgg tggacttcga ccacgaccgg ctggagttcg agcacgagga cagcggcacc
     4441 cgcgagaccg tggcactgac gccctgcatc ggcgcggacg gcgccggctc ggtgctgcgc
     4501 aaggcgctgg cagtgctgcc gggccacacg gcaagcgagg aaattctctc ccacggctac
     4561 aaggagctga gcgtcctgcc caacgggctc ggccgccacc gcatggagag caatgcgctg
     4621 cacatctggc cgcgcggcgg ctacatgctg atcgcgctgc ccaatcacga cggcaccttc
     4681 accgtgacgc tgttcctgcc gttggagggc gagccgtcgt tcgcgagcct cgactcaccg
     4741 gcggcggtgc gcgcattctt cgcggccgag ttcccggacg cattgccact gattcatgat
     4801 ctcgaggccg catttttcgc caacccgacc ggcatgctgg gcaccgtgcg ctgccaaccc
     4861 tggaatctgg acggccgggt ggcgctcctc ggcgacgcgg cgcacgccat cgtccccttc
     4921 cacggccagg gcatgaactg cgccttcgag gattgcctgg agctcgacgc ctgttttgat
     4981 gaatcgggtg gcgactggcc acgcaccttc gagctgctgt cggtgcgccg caagccgaac
     5041 gccgacgcga tcgccgacat ggcgctggaa aactacgtgg agatgcgcga ttcggtgcgc
     5101 gaccccggtt tcctgctccg ccaggaagtc gccttcgagc tggagcggcg ctttccggag
     5161 cgtttcgcgc gacgctacgg actggtgatg ttccgtgacg acgtgcccta tgcggaggcg
     5221 cagcagcggg gacgcatcca ggagcagatc ctgctcgaga tcacgacggg catcgatgac
     5281 gccgccgccg tggattacga ccgtgcgggc cggctgatca agcggcggct gttcccgctg
     5341 ccaggacact gactggcagc acgcccgcgg gagatcgtca cggcccggac gcggtgcacg
     5401 ccagccggtc gccggctgcc gccaccacgc gccggcagcc cttgggatag ccgcgcgcct
     5461 cccagagcgg gaccatgccg agcacctcgg ccacggcgaa gaaacgcgga tcctcgcgga
     5521 gtgcgccggt ggacggtagc caggccgacc gcagccagcc ctgctggttg tacaggcgcc
     5581 cgctcaccgc caggtcgaga agcaggtcga gataccgatc cgtcatgccc agcgcaagcc
     5641 attcctcggg cggccgagcg gaaccctcca ggtcgagcac ggagtccgcg cgttccggtt
     5701 cgcgcagggc aacaatgaag gcggcgacgg aggcctcgta gggccctggc tgggccagca
     5761 gctcgcgcag caatgccacc gcccggtccg tttccccccg gttcgccagc tcgatggcat
     5821 gaacccagag ccccgcgggc cagttgagct cggccgcctt gcgcaagctg gcctccgcct
     5881 cggcgtcccg tcccagcgcc aggcggacca tcccgagata gccgtggttg atgccgacca
     5941 gggggtccat gttcgcggcc cgcgcgagcg cggtctccgc ttcctctagg taaccggata
     6001 cgaacagcgt caggccatgc cacatggtcg gcgtggaatc ctgcgcgctc agttcgctgg
     6061 cccgctgcaa caattccagg gcctcgggct tgccctcttc acgcagcagc atgccctgca
     6121 gcccgatgac catcgggtgt tccggcaaca gtgcccgggc ccgctcgaca ccggtccggg
     6181 ccgccgggat catcgcatcc tggtcgaagg atccgacgtc ataaccgggc gccacgaaat
     6241 tggcggcggc gagatagacc caggcctcgc cgaactcggg atcctgctcg accacgtagt
     6301 tgagatcagc gatggcgcgg ccgaggtccc tgcgctggtg gaagagcgcg cggccgcgca
     6361 ggaagcgctc gtaggcttcg atgtccgcag ttgcagcgcg taccgatacg cggcgcgtgc
     6421 cgagaatctc gccgagggcg ccggtgatcg cctgggcgat ctcctcctgc accttgaaaa
     6481 tgtccaccag ttcccggtcg taggtctgcg accagaggtg gccgtcggtg tccgcccgga
     6541 tgagctgtgc ggtgatgcgt acgcggttgc cctgcctgcg caccgaaccc tcgagaacgt
     6601 gcagcacgtt cagctcaccg gcgatctgcg gaatgggaac acccttgccg cggaatgaga
     6661 aggctgaagt gcgcgacgca acgtgcagcc cctcgacgcc ggccaggatg ttgagaattt
     6721 cctcggaaat accctccgca aaataggcgt tttcagggtc cgggctcatg ttgtcgaagg
     6781 ccagcacggc gatggaccct gccggccggc cgccgtcatc agccaccgcc gccggatcgg
     6841 acgccgccct gtccggcgtg gtgaccgcga accgctccgc cacgaacatg ccgatgacga
     6901 cgagcagccc gagggcgatc acccagtcca acacccgacc gccgggcggc gcggtccctg
     6961 cgacggcatg ctcggtgcgc ttcacgccct ccggcgtcag ctcgaaggcc caggccatga
     7021 acagcgcgac cgggaatccg agggccagca ggacgatcac cagggtgacg gtccattcag
     7081 gcaggccgag acgcgggaac actgtgtcac tgacctcgat cagcagccag gccaccacgg
     7141 cataggcccc gcccacgcgg atcacgttgc gccgtcgcag ttcggcaaac aggctcaagc
     7201 cccgctcccg cgccgcgagt acttcgccgc cagcgcctgg atcgccggat gatttcgatg
     7261 cgcgtcgaac acggggtcga gcatcaggcc gtcgaaatgc cagtacttca tctcgagctg
     7321 caggtattcg tccatggccg cggcgagctc cgtcgagtcg gcaaccatgg ccagcaccaa
     7381 cagctctcgc atgcgctgct ccgatcgaat gacgacgtcg cgcgtcggga tggtctgcga
     7441 cagggcctgc cgggtcgctt cgcgagccgc cgcaccttcg ccgcgaatgg caagtgcctg
     7501 cgcgcgcgcc atcaacgcac gatagtcatc gggatgctcg gccaggatgc ggtcggtcac
     7561 cgccagcgcc ccgccagccg ccttcgccgc ggcttgctcc ccgccgcgcg cccgctccag
     7621 gtaggcgcgt gacagggact cgatgcccag cccgaactgg tcctcggaca agtctccttc
     7681 atagctgtcc agcacggcag cggccttgtc gaagtcacgc tcgaaatagg ccaggatgaa
     7741 ggtatttccc agcgccgcgc cgaccagcgt gaacgttctc ggcgtgtcgg ccgcgaaggc
     7801 cctgcggatc ggctccaggt cccccaacac ctgcatgcgg gcgttcggat accagaaccg
     7861 gttggtcgaa tggtcatcag gcagcgcatc gagctgctcc tgccagcgca gcgccgcctc
     7921 catgttgccg gtgagccagt acgtctcgac caggccgcgg atgacttctc ccgagcgggg
     7981 atcgagcaac accgccgagt ggagcgcgtc cagggttgcc gcggtccggc cggcccgccg
     8041 catgatgtag gcgcgcaggc tgaccgcttc ggcgctgccc ggaaggccgc gcgtggcgag
     8101 gtcgagattc ttcagcgcag cctcgtagtc caggtgggcc cgatagtgat actccgccat
     8161 ggcgagccgc agctgaggat cctccggctg caacgccagg gcacgatcga gccagcgccg
     8221 cccctcggca cggtcggcgt cgcgccgcgt ggtgaaccag taaaggttga cgaactcccg
     8281 gcccagttcg cccatcgcca gcgcgaatcg caaatcgtgc tcgagcgcct ggcggtacag
     8341 cgcgatgcgt tcccgtatct gctcctcttc ccagtcatgg cgaaacgccc gggcgcgaag
     8401 gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagcg
     8461 cctggcccag ggcggccgag atcgcggccg cgatctcgga ctggatgtcg aagacgttct
     8521 cgggcgtcag ctcgcggtcg aaggtgtccg cccacaggtg ggcgtcgctg gcggcgtcga
     8581 tgagctgcgc attgatgcgc acacgccggc cggcgcgctg cacggcgccc tcgaggatgg
     8641 tcgccacgcc cagctcctcg ccgatctggc gcaggttctt ggtggtgtcg cggtactcca
     8701 tcatcgaggt gcgcgagatg accgtcagtc cgccgttgcg cgccaactgg gtcaacaggt
     8761 cgtcgtggat gccgtccacg aagtaggccg tgtccggctc ggcactgcga ttggtgaacg
     8821 gcagcacggc gatcgagcct ggcggggcct cgaaaggcgg cggttcctgg cgcactcgct
     8881 gtgcggccgg cgggtccgtc acggcaggtg ccgaagtttg acgttcgccg ctgccgtccc
     8941 gggtgccgct caacgccggt ccaaggtgct cccaggcgag caaggccacc accaggacga
     9001 ccgcaccgag ggtaatcacg tccagccgtc gcgcctgcgg gctgacgtcg cgagggttgc
     9061 tgccgtcgtc gcggcgcacg ccctccgggc ccacctcgaa cacccacgca agcgccagcg
     9121 ccggcaccag acccagcagc agcagggcga tcagcgcctt gccgacccac tcaggcgcac
     9181 cggtgaagcc gagcatgatc tccgcaacct ggaggatcag ccagccaacg acgatgtatg
     9241 cggcggcgac gcggaacacg ttgcgccgct tgagttcggc ccacaagccg ctgctggagc
     9301 tcatggcgca gcccttctga tgggtttcag acccattcca gcgtacggca ggcggggcac
     9361 gcggttcaat cgcatgagaa atcgtcctcg cccaccggcc ggcacatcgg cgggtagccg
     9421 tggtcccgcc agtagtcgaa gacgcccgtc ccccgaatgt actccttgaa ctgcggtgac
     9481 ctacggaaac ctgcgtactc cggcccccag acgagcattc cataatccgg cagcatgtcg
     9541 aaggccccca gcgggacgag cagcaaggcc gtgaaggagg cggcgtccac gggcctcgcg
     9601 ccctggaact cccgcaatgc cgccagcaac tcggcatgat cgccgtcgag gtcacgaaac
     9661 gcttcgtaga gatcccggtg gcggtgccag cccggcagcc acttcgactg gttggccagc
     9721 aacatgaacg cggtctgctc ttcgaaatgg gccagcaggg cgaagttgcc gtactcggaa
     9781 accaccgcgc cctcggccag cgcctcctgc aggcgctcga acgcctcgtc cggacggccg
     9841 agggtccaca agatatcgta atggttctcg cgacaggggc cgaagcgcgg tgcgacttcg
     9901 atacaacgta cgaaggtctg cagtgcatcc tcgagccggc cgacgaggcc gagagacaat
     9961 ccgagccagt tgtgtgccga actgttatgc ggatcgagct ccacggcgcg ctcgaggtcc
    10021 cggatgatgg tgccgagatc atgccgctgg agcaatttcg cggctgcacc cattcgcagg
    10081 ttggccagtg tggccagcgc catgccgctg tccggctcca gggccaaggc cctttccgcg
    10141 aactgaattc cccggcgttc atgttcatcc cgggacaaac gcgtgtagcc gtactcgacg
    10201 atcacggtct gcactgccgc ccgcagttcc caggcattga cgaattcggg atcctgctcg
    10261 agcgcctggc gcagcaggtc atcggcgaca tcgagatcga tgcggccctg gaacaggccg
    10321 cgcgcgcgca ggtacatgtc ataggccgtc aggttttccg tcggctgggc cagccggacc
    10381 tcgtcctcga tctcgatgcc caatgaatcg accagcgccg cgacgatcgc cgaggcgatc
    10441 tcctcctgga tggcgaagac attttcggcg gtcagcgggc ggtcgaaggt gtcggaccac
    10501 aggtgccgat ccacctccgc gtcgatcagc tgcgcggtga tgcgcaaggt gttgcccgcg
    10561 cggcgcacgc tgccctccag cacgtggcgc acctgcaggc gctcggcgat cacgggcatg
    10621 cccaggcttt cctggccctt gaacccgaac gaggaagtgc gggaagcgac ctgcaggccc
    10681 ttcacccgca ccaacgcgtt gagaatttcc tcggccatgc cgtctgagaa gtactcctgg
    10741 tcggcaccgg gcgaaaggtc ggcgaacggc aacaccgcaa tggacgcggc gttgaccatg
    10801 cccgcctcat cggcaaccgt cgccggcgcc ggcgcgacca ccggtgcggc gaccggcggc
    10861 gccgggcgca gccgttcaac ggccatgaag ccgatggcca ccaccgccat cgccagcgtc
    10921 gcccagttga gcttccgtcc ggtgctcgag ccccgcatgt cggcggccga catctcggca
    10981 tcacgcttga tgccctcggg cgtgagttcg tagatccacg acaggcccag cgccggcacc
    11041 aggccgatca ccagcagcac gaccagcccg cgcagcacgc cgtcggggac gtcgaacagt
    11101 ggcagcaccg tctcggccac ctcgatgacc agccacgccg tcacgacgta gaacagtccc
    11161 acgcggaaca cgttgcggcg cttgagttcg gcgaacaagc ccggccgtgc gctcacggca
    11221 ccaccacctg gatatcgaga ccgggcgcac gcgccagctt cgcatcgcgc gtgaccagcg
    11281 tggcgtccag caggcgcgcc agcgccacat acgctgcgtc gtaagcagtc atgttcttgc
    11341 gcaatgacca gatggcgtcg cgaaggctgt agtggcgata gcggcggatg cgcagggcac
    11401 gcaacagctc aatgacatct gcacgggtcg gcggaatggc gccgtcattg tccagccggc
    11461 gcagcgcctg cagcacctct acatcgagca actcgggcgc agccaggacg ctgtcctcag
    11521 cccacaagag cgcttcgatc ggctgcggcc ggaatcgccc gatcaggtcg acgacgaccg
    11581 atgcatcgac gaccaggacc tgactcacgc tgcgtcacgc tcgcggcgga tcagcccggc
    11641 ggacgatttc ttcatggcga atggctcggc ctgctgcagc ctttcgcgca gctcctgctc
    11701 cgaagggagc gccataatgg cctgcagctc ctccagaatc agttcgctca tgctcttgcc
    11761 ggccagcgcc gcgcgcgctt tcagcgccct gtggatcttc accgggacat tgcgaatctg
    11821 gatcatgctt gacatgcgac caccctcctt tcatgtggat cacatgatag catgttagtc
    11881 acactcgaaa tcgtcatcac cgacaggccg gcacaattgc gggaagcggt gctcgcgcca
    11941 gaaggccagg accccggtgt cgcggatgat ctgcctgaaa gcctcagtct ggcggaaccg
    12001 ccggaacgag tcgctccaga gctgcgcacc cagcacgtgg atttcgttcc tgcccagggg
    12061 taccagcagc aagtccatga tctgaccgcc gaggtccgtc ggggtttcgg gcaggtattg
    12121 cactgcctcg gtcaccaccg ccgagtgatc gccccgagga tcgagatggg cggcatagag
    12181 ttctgccagg cgtggaaagc cttgcagcac ctcgggatga ttggcggcct ccatgaaaag
    12241 aatgtccgat tccgtctcca cccagagagg gatcaggtgc aagccatata gcgacaaacc
    12301 ccggccgagg tcccgccggt acatggccat cgcttcgtca aaacggccca gcgcaaccag
    12361 gacgtagggg cgattgttga cgcaaggcaa atacaggggc tcgagcgtga cgcactgctc
    12421 gtaggccgcc aacgcctcct cgaggaggcc gatcgattcg agcatgttgc ccagccagat
    12481 gtgcgcgctg gcattgttcg cgtcggcgtc gatcgcccgc tcgaaatagt cgagtgccct
    12541 tgcccagtcc gcgaagcctt ccaggtattg ccccttttcc atcagcagct ttcccagcgt
    12601 ggcgagcgcc agtgaactcc ctgggttcag ttccagcgcc ctggccgcat actcgcgcgt
    12661 ccgcgcctcg gcagtttcca gcagctcgct ggagtgaccg taaccgatcg ccagcgtggg
    12721 aatgggcgca cgcagttccc aggcgcgcgc aaaggacgga tcctgctcca gcgctctttc
    12781 cagcagcgcg tccgcccggt cgaggtcctt gcgctggtgg tagagtgtcc gcgcttcgag
    12841 atacagctcg taggcgtcga tatcgtcggt gccgccggcg gacgagacca gtttctcgcc
    12901 gccgagcttc atcgccaccc cgagcgcccg ggtgatcgcg gccgcgattt cgtcctggat
    12961 ctggaaaacg ttctcggccg tcagagaacg gtcgaaggtc tccgaccaca ggtgacggtc
    13021 gttggaggcg tcgatcaact gcgccgtgat gcgcagcgta tcgccggcct tgcgcacgct
    13081 gccctcaagg acgtggcgga cggcaagggc ctgtgcaatc tcggggatac cgatctcgcg
    13141 ccccttgaac tggaaggccg aagtgcgcga cgcgacggcc agaccatcca cacgggtcag
    13201 cgcattgaga atctcttctg cgatcccgtc ggagaagtat tcctggtcgc cgccgggaga
    13261 caggtcggcg aaaggcaaca ccgcgatcga ggcggcgtcg acgttatccg gcatgttcgt
    13321 gactgccgct ggcgccttgg ccgcggacgc cggtgtcgcc gacggccccg cgaccggcgg
    13381 cgcggggcgc atccggtcca ccgccatgaa gccgatggcc accaccgcca ccgccagcgt
    13441 cgcccagttg agcttgcggc cggtgtccga cgactgtggg ccggcgacag agggcccgac
    13501 gtcgcgcttg atgccctcgg tcgtaagctc gtagatccag gacaggccca gcgccggcac
    13561 cagcccgatc accagcagta ccaccagccc gcgcagtacg ccgtccggca cgtcgaacag
    13621 cggcagcacc gtctcggcca cctcgatgac cagccacgcc gtcaccacgt agaacagccc
    13681 cacgcggaac acgttgcggc gcttgagttc ggcgaacagg ctcaacacga gtttccttga
    13741 catactgcca tactggtaat atggttttcc atatgaaaac caccctgaac atcgatgaca
    13801 gcgtgatgca gcgcctgcgc gaagcggccg cgcgccaggg ccgcacaatg tccgagctgg
    13861 tcgaggccgg tctgcgccga gtgctcgaag agtcggaaga aaccaccaac caggtgcgcg
    13921 agccgttgcc gcaatggcaa agcggcggcg cgtacgtcga cgtggccgat cgcgatgcac
    13981 tgcaagaccg gatggagcga gactgagcgc cggccgtgtt cgtcgtcgat accaacgtgc
    14041 tgttgtatgc ggctgatgtg gacagcgagc accacgctcc ctgccgaacg ctgatcgagc
    14101 aggcccgggc ccaggcggcg ccctggttcc tgacctgggg gatctgctac gaattcctgc
    14161 gcgtcagcac gcacccgcgc gtgtaccgca gcccgtggac gaacgccgcc gcctggcgct
    14221 tcatcgacac gctgctggcc tctcccagcc tgagcgtcct ggcagcgacc cgccggcacg
    14281 cggcc
//
LOCUS       DAOMPG010000040        13887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1892_length_13887_cov_4.490818, whole genome shotgun sequence.
ACCESSION   DAOMPG010000040 DAOMPG010000000
VERSION     DAOMPG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_13887_cov_4.490818"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLS34_03540"
     CDS             <1..785
                     /locus_tag="PLS34_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP86577.1"
                     /translation="RTPRSNPATYSGLFTPIRELFAGTAESRSRGYKAGRFSFNVKGG
                     RCEACQGDGVLKVEMHFLPDVYVACDACKGQRYNRETLEIRYKGRNIHEILEMTIEDA
                     LEFFANVPVVRRKLQTLMDVGLSYVRLGQNATTLSGGEAQRVKLAKELSKRDTGKTLY
                     ILDEPTTGLHFHDIAQLLGVLGRLRDHGNTVVVIEHNLDVIKTADWIIDLGPEGGSGG
                     GRLVAEGTPEQVAAQRGSFTGQYLRPILAAAAPDKPRRRRRA"
     gene            790..>975
                     /locus_tag="PLS34_03545"
     CDS             790..>975
                     /locus_tag="PLS34_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HRP86578.1"
                     /translation="MGKRFALIALLLAVAALLTLLERGPLGPAPGPASPSQSAPAGLP
                     PEAVETLRLIERGGPFPH"
     assembly_gap    976..1019
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1020..1164
                     /locus_tag="PLS34_03550"
     CDS             <1020..1164
                     /locus_tag="PLS34_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRP86579.1"
                     /translation="YYREYTVRTPGAGDRGARRIVTGGDPPEVYYYTADHYRSFRRFE
                     VPR"
     gene            1161..1592
                     /locus_tag="PLS34_03555"
     CDS             1161..1592
                     /locus_tag="PLS34_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRP86580.1"
                     /translation="MNTVGATWRLTQPADNGVLWLAAAEVDALCAAGDAAGLHCMRVV
                     LGDLDGKAALLERLADALAFPDWFGHNWDALADCLGDLSWLPASGYLLVLEDCRRFAR
                     RSPGDYGILLEILADAAGTWRARAVPFRVFLSMAAPHGPLA"
     gene            1589..3151
                     /locus_tag="PLS34_03560"
     CDS             1589..3151
                     /locus_tag="PLS34_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012943578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRP86581.1"
                     /translation="MSDTAQDIRKADRDLDGPGAYGERPEIRFRGGKAASAVPILFFI
                     AWAIFQSGLLGISDTAGLIVGMLVGLTLGMLLVRGSWTVYANAIFAGMAQKVAVTAIV
                     AWLWAGMFAATLQVGGFVNGLGWLAEAAGMGPAVFPAVTFVLAALLATGIGTGYGTVI
                     AFVALFFPAGVVLGADPLLMFGAILSGAVFGDNLAPVSDTTIVSAVTQDSDVGGVVAS
                     RFKYAIIAAVLAFFAYLVAGSMSTGAPVDADAAALFSAQSNPRGLLHLLPMGIVLFSA
                     IRGRHIIEAISWGLIAAIAVNLLFGLATGSDMLVFRVADGSWAAGFAHWPLVTAVPAG
                     DAGVGGSLYAGAIGYAELAVLILLVLAGAHVMIRGGGFHALQDWLLRKVARTVRSAEV
                     TMVLATATINAAITINTAAQIAIAPYIATIGKRFNLNGYRRANILDANTSALGYIFPW
                     AGGVLVGYTQMTGLADTYPWFTPAMVVEPIQAWPWVFHGWLLVAVFLFAAWTGFGREY
                     TTDRVSKGGAGL"
     gene            3148..3402
                     /locus_tag="PLS34_03565"
     CDS             3148..3402
                     /locus_tag="PLS34_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86582.1"
                     /translation="MIAGLFANWRFRSSRPVFTPGEEIRAYLTGFDPATGEGTARIGD
                     TVLTIKGCHAAQLDRLVELQIEHFDAGRGTARLAHPHAVD"
     gene            complement(3436..4506)
                     /locus_tag="PLS34_03570"
     CDS             complement(3436..4506)
                     /locus_tag="PLS34_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86583.1"
                     /translation="MPRPGLLALLWVVAAAGLLVLMLGQPQQRWAPPTPTEVVLDGEH
                     YRLDAPQLEWLREFSVLHFSAGRAEARRLLESELDGGLEDVFAAVRARLPEFADWYYS
                     LGGEYSRLGMAALAGLNLADGDFIARRASALLFPADDWEEALAGLDRRMLAKLEAHRQ
                     RVRADWLAQLSDRLAAHRVPAPLHAGDGTRTAPLQLEGFIRQLVAEEQRAFAARVRLS
                     SVTGGATAGVVLWRQAAARAATATGRAVAARGASRGAARAGAAATGGLAVCAATGPAA
                     AGCALLAGAVAWVATDWALLQIDELRHRDELVAALEAGLEQLRTQMHAEVLAAYDVLS
                     RELETRENTAITTEFRPARAGR"
     gene            complement(4529..5323)
                     /locus_tag="PLS34_03575"
     CDS             complement(4529..5323)
                     /locus_tag="PLS34_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86584.1"
                     /translation="MPDAARLWRDLLCLLGLVALAWLYHVFARVLPSIGLAATWALFA
                     TLIATALWQRARIRRRAFLAVYIAAASPLQRLLRGGPLLALRPLLLGGLLAILLMVAL
                     ARTSDHWAWVALMASGPALLLLRARLQRRLQGHAGALYLPILSWRAAAWPVGAALMAV
                     LAWLGFQQAYPDFAGVSLERAVWHMVDQERARSGPAEVLLQAAAAKDGVRLWLAQQLM
                     PAPATSLLQLLGWLLILAEEVLFVWSYLLLCHGTLLGAGAIDRSDH"
     gene            complement(5331..6545)
                     /locus_tag="PLS34_03580"
     CDS             complement(5331..6545)
                     /locus_tag="PLS34_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP86585.1"
                     /translation="MSMPPHNLAADLRGASRLIVDAGSQVTDLVEAMHGTIAGLAGLI
                     PGSSNQRTRGVTGLVYRCVGGGMRLVGGGLDRLLASSAMLVGTRERPASHETALAVLN
                     GVLGDHLAATGNPLAITMSLRRDGVPLHLEAGAIRRTIPAPGPRVLIQAHGLCMHPGQ
                     WARTGRDYLAELGHALGYTSLHLHYNSGLHIAANGRTLAALLESLSGHWPVPLEELAI
                     VGHSMGGLVVRSAYHHGEAAGHEWPRRVGKLVFLGTPHHGAPLERAGNGFDALLGWSP
                     YTRPFMRLGRTRSHGITDLRYGILLEEDWQACDRYAPMWKDPRTPLPLPAGVACYAAA
                     ALRAERDDTPAARLVGDGLVPLASALGEHEDARFALRFPAANRWIGTGLGHLDLLSTP
                     RVFAQVGRWLAQ"
     gene            complement(6562..6711)
                     /locus_tag="PLS34_03585"
     CDS             complement(6562..6711)
                     /locus_tag="PLS34_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86586.1"
                     /translation="MTLCPVALAAGCRKCAIFSVCPLKGVIGDYRKGDDASPKRDPKH
                     EQKPR"
     gene            complement(6761..7102)
                     /locus_tag="PLS34_03590"
     CDS             complement(6761..7102)
                     /locus_tag="PLS34_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRP86587.1"
                     /translation="MRNLPINEGAFTVLCVFDCNPGESERLAREIAAWVEARVRFHPG
                     FLSSMIYLSEDGGRIVEFFQWARGEDWEAYRASEDGRAAVAQLAGRNPKIEFLEMIAA
                     AAAPPPGHCAA"
     gene            complement(7124..8830)
                     /gene="ggt"
                     /locus_tag="PLS34_03595"
                     /pseudo
     CDS             complement(7124..8830)
                     /gene="ggt"
                     /locus_tag="PLS34_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(8901..9749)
                     /locus_tag="PLS34_03600"
     CDS             complement(8901..9749)
                     /locus_tag="PLS34_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86588.1"
                     /translation="MLAILVAAGCDRIDTLRLAYANNGTPIEWPSGVPAVELALETTV
                     DGRIWIPVSVDDNPPVPFLLQASAGAVALTGARASGFGPVGAGSLALRDDVLPGIRGG
                     LLIKQRRFALGSLVLDGQSLLLVAPEEWPHGTPGGAAAGVLGYDLLRRFVVELDAGNS
                     RLAVYRAGRLDVGGMPEVYRLAVLQRVPYFEVSMESGGPAARWLRLQFEPAAPVGICL
                     DEEPDPGVVTLAGRTIPIDVAPCPAVGPRGQRQGRDGVFGAGALRGLVVAVDYERGQI
                     GFRARD"
     gene            complement(9767..10318)
                     /locus_tag="PLS34_03605"
     CDS             complement(9767..10318)
                     /locus_tag="PLS34_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP86589.1"
                     /translation="MSIPDHREYRALGYMIDGTPFFLRALREWDRGAILNLFTRSSQQ
                     SRYFRYFETKNSLSDAELDYYTRLDFYRHVALAAEAPDEDGIIAVGRYIEVQPETDPR
                     RAEVAFLVRDDYQGRGVATQLLKHLIRIARDNGIQQFEAEMLPSNTKMIEVFDHSGYQ
                     LKRSREPDSVRVVFPIHDERIPF"
     gene            complement(10330..11232)
                     /locus_tag="PLS34_03610"
     CDS             complement(10330..11232)
                     /locus_tag="PLS34_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRP86590.1"
                     /translation="MTTDLSTCLVVGISSRALFDLRREDALFVSAGLDAYRAEQLGRE
                     DQVLPPGTGFPLARAVLALNGPAAARRAEVVVMSRNSTETSLRIFNSIRHYGLDITRA
                     ALSGGAPLAPYLRAFKVDLFLSCYEDDVRRALDAGVAAALLYDRPENPLEAADEIRIA
                     FDGDAILFSDESERIYQADGLEAFARHEQARALEPLAEGPFARLLHKLSALQQAAARD
                     GITPIRTALVTARGGPAHERVIRTLLAWGIVVDEAFFLGGVPKTEILEAFRPHIFFDD
                     QAAHCERAAARVPTGRVPVGTSKT"
     gene            complement(11412..11786)
                     /gene="rplQ"
                     /locus_tag="PLS34_03615"
     CDS             complement(11412..11786)
                     /gene="rplQ"
                     /locus_tag="PLS34_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRP86591.1"
                     /translation="MRHRKAGRQLGRKSPHRTAMYRNMASSLVLHETIRTTLPKAKEL
                     RKVVEPLITLAKQDGVAQRRRAFDRLRDKEVVGKLFNELGPRYRARPGGYLRILKIGN
                     RPGDSAPMALVQLVDQPEKTAE"
     gene            complement(11816..12814)
                     /gene="rpoA"
                     /locus_tag="PLS34_03620"
     CDS             complement(11816..12814)
                     /gene="rpoA"
                     /locus_tag="PLS34_03620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRP86592.1"
                     /translation="MQASVNEFLRPRVVKVQPESRTRAKVTIEPLERGFGHTLGNALR
                     RILLSSMPGCAVVEAEIDGVLHEYTSIEGVQEDVVDILLNLKQLAVKMHSRDEIDLNL
                     SKKGPGVVTAADIETGHDVEVINPDLVIATLTKAGNLSMRLKVRRGRGYQPVTQRPAF
                     EEQSGPIGRLQLDASFSPVRRVAYTVDSARVEQRTDLDKLIIEIETNGTIDSEEAIRR
                     AGAILKDQLAVFVDLQGEGLGPEPGDKAIDPILLKPVDELELTVRSANCLKAESINYI
                     GDLVQKTEVELLRTPNLGKKSLTEIKAVLENHGLQLGMRLDGWPPASLRHDDERVA"
     gene            complement(12834..13454)
                     /gene="rpsD"
                     /locus_tag="PLS34_03625"
     CDS             complement(12834..13454)
                     /gene="rpsD"
                     /locus_tag="PLS34_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRP86593.1"
                     /translation="MARYLGPKCKLSRREGTDLFLKSSVRPLESKCKLEVPPGGAPQR
                     RPRISDYGLQLREKQKLRRMYGVLERQFRNYYKRAAQQKGSTGENLLKLLEGRLDNVA
                     YRMGFASTRAEARQLVGHKGVSVNGQVVNIPSYQVNPGDEIAIRDKARKQLRVQNALQ
                     LAGEAGFPEWVEVDLTKMSGVLKQLPDRDDILPDINENLVVELYSK"
     gene            complement(13475..13864)
                     /gene="rpsK"
                     /locus_tag="PLS34_03630"
     CDS             complement(13475..13864)
                     /gene="rpsK"
                     /locus_tag="PLS34_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRP86594.1"
                     /translation="MAKQAPKVRKKIKKQVVDAIAHIHASFNNTIVTITDRQGNTLSW
                     ATSGGCGFRGSRKSTPFAAQVAAEKAGNAAAEYGVKNVEVRVKGPGPGRESAVRSLNA
                     VGFRVTNIEDVTPIPHNGCRPPKKRRV"
BASE COUNT         2119 a         4884 c         4655 g         2185 t
ORIGIN      
        1 gccgcacgcc gcgctccaac ccggcgacct acagcgggct gttcacgccc atccgtgagc
       61 tgttcgcggg caccgccgag tcgcgctcgc gcggctacaa ggccggacgc ttcagcttca
      121 acgtcaaggg cggccgctgc gaagcctgcc agggcgacgg cgtgctcaag gtggagatgc
      181 acttcctgcc cgacgtgtac gtggcctgcg acgcctgcaa gggccagcgc tacaaccgcg
      241 agacactcga gatccgctac aagggcagga acatccacga gatcctcgag atgaccatcg
      301 aggacgcgct ggaattcttc gccaacgtcc cggtggtgag acgcaagctg cagacgctga
      361 tggacgtcgg cctgtcctac gtgcggctgg gccagaacgc caccaccttg tcgggcggcg
      421 aggcgcaacg cgtcaagctg gccaaggaac tgtcgaagcg cgatacaggc aagacgctgt
      481 acattctcga cgagcccacc accgggctgc acttccacga catcgcgcag ctgctgggcg
      541 tgctcggccg gttgcgcgac catggcaaca ccgtggtggt gatcgagcac aacctcgacg
      601 tcatcaagac cgcggactgg atcatcgacc tcggtccgga aggtggcagc ggcggcggcc
      661 gcctggtggc ggaaggcacg ccagagcagg tggccgcgca gcgcggctcg ttcaccggcc
      721 agtacctcag gccgatcctc gctgcggcgg cgccggacaa gccacgccgc aggcggcgtg
      781 cctaggccga tgggcaagcg gttcgcgctg atcgcgctgc tgctggccgt cgcggccctg
      841 ctcacgttgc tggagcgggg accgctcggg cccgcgcccg ggccggcgag cccgtcgcag
      901 tcggcacctg ccggcctgcc gccggaagcc gtcgagaccc tgcggctgat cgagcgcggc
      961 ggccccttcc cccacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1021 tactaccggg aatacacggt acgcacaccg ggggcgggcg atcgcggcgc gcgccgcatc
     1081 gtcaccggcg gcgatccgcc cgaggtgtac tactacaccg cggatcacta ccggagcttt
     1141 cgccgtttcg aggtgccgcg atgaacaccg tcggtgcaac ctggcggctc acacagccgg
     1201 ctgacaacgg cgtgctgtgg cttgcggccg ccgaggtgga cgcactgtgc gccgccggcg
     1261 atgctgccgg cctgcactgt atgcgtgtcg tgttgggcga cctcgacggc aaggccgcgt
     1321 tgctcgagcg gctcgccgac gcgctcgcat ttcccgactg gttcggtcac aactgggacg
     1381 ccctggccga ctgcctcggc gacctgtcct ggttgccggc cagcgggtat ctgctggtgc
     1441 tggaggattg ccggcgcttt gcccggcgct caccgggcga ttacgggatc ctgctggaga
     1501 ttctcgccga cgcggccggc acgtggcgcg cgcgtgcggt accctttcgg gtattccttt
     1561 ccatggcggc gccgcacgga ccattggcat gagcgacacg gcacaggaca tacgcaaggc
     1621 cgaccgggac ctggacgggc cgggcgcata cggcgagcgg ccggagatcc gttttcgtgg
     1681 cggcaaggcc gccagcgccg tgccgatcct gttcttcatc gcctgggcga tcttccagtc
     1741 cggcctgctc ggcatcagcg acaccgccgg gttgatcgtc ggcatgctgg tcgggctcac
     1801 gctgggcatg ctgctggtgc gcggcagctg gaccgtttac gccaacgcga tcttcgccgg
     1861 catggcgcaa aaggttgcgg tcacggcgat cgtggcctgg ctgtgggcgg gcatgttcgc
     1921 cgcgaccctt caggtcggcg gcttcgtcaa cgggctcggc tggctggccg aggccgccgg
     1981 catggggccc gccgtgttcc cggccgttac cttcgtgctg gcggcgctgc tggccaccgg
     2041 catcggcacc ggctacggta cggtgatcgc cttcgtggcg ctgttcttcc ctgcgggcgt
     2101 ggtgctcggc gcggacccgc tgttgatgtt cggtgccatc ctgtccggcg cggtgttcgg
     2161 cgacaatctc gcgccggtga gcgacaccac catcgtcagc gcggtgaccc aggactcgga
     2221 cgtcggcggc gtggtcgcga gtcgcttcaa gtacgccatt atcgccgccg tgctggcctt
     2281 cttcgcctac ctggtggccg gctcgatgtc gacgggcgcg ccggtggacg cggacgccgc
     2341 ggcgctgttc agcgcccaga gcaatccgcg cggcctgctc catctcttgc cgatgggcat
     2401 cgtgctgttc agcgccatcc gcggccggca catcatcgag gcgatttcct ggggcctgat
     2461 cgcggcgatt gccgtgaacc tgctgttcgg cctggcgacc ggcagcgaca tgctggtgtt
     2521 cagggttgcg gacggcagct gggccgccgg cttcgcccac tggccgctgg tgacggcggt
     2581 cccggccggc gatgcgggcg tgggcggcag cctgtacgcc ggggccatcg gctacgccga
     2641 gctcgccgtg ctgatcctgc tggtgctggc cggcgcgcac gtgatgatcc gcggcggcgg
     2701 tttccacgcc ctgcaggact ggctgttgcg caaagtcgcg cgcacggtgc gctcggccga
     2761 ggtgaccatg gtgctggcga ccgccaccat caatgcggcg atcaccatca acacggccgc
     2821 gcagatcgcc atcgcgccct acatcgccac catcggcaag cgcttcaatc tcaacggcta
     2881 ccgccgcgcc aacattctcg acgccaacac ctccgcgctc ggctacatct ttccctgggc
     2941 gggcggcgtg ctggtgggct acacgcagat gaccggcctg gccgacacct atccctggtt
     3001 cacgccggcc atggtggtcg agccgatcca ggcctggccc tgggtgttcc acggctggct
     3061 gctggtcgcg gtattcctgt tcgcggcctg gaccggcttc gggcgcgagt acacgacgga
     3121 ccgggtcagc aagggagggg ccggactgtg atcgccgggc tgttcgccaa ctggcgcttc
     3181 cgcagcagcc gacccgtgtt cacgccgggc gaggaaattc gcgcctacct caccggcttc
     3241 gatccggcca ccggcgaggg gactgcgcgg atcggcgata ccgtgctcac catcaagggc
     3301 tgccatgccg cgcaactcga ccgcctggtc gagctgcaga tcgagcactt cgatgccggc
     3361 cgcggcaccg cgcgcctggc tcacccgcac gccgtcgatt gagccgacga cgggggccgc
     3421 gtccgggcgc cgcgatcagc gaccggctcg cgccggccgg aactccgtcg tgatggccgt
     3481 gttctcgcgt gtctcgagct cccggctcaa cacatcgtag gccgccagga cttcggcgtg
     3541 catctgcgtc cgcagctgct ccaggcccgc ctccagcgcg gccaccagtt cgtcgcggtg
     3601 gcgcagctcg tcgatctgca acagcgccca gtcggtggcg acccaggcga ctgcgcccgc
     3661 cagcagcgcg cagcccgccg ctgccggacc ggtcgccgcg cacaccgcca gcccgccggt
     3721 ggcggcggcg ccggcacgcg ccgcgccccg gctggcgccg cgcgccgcga ccgcacggcc
     3781 ggtggcggtc gccgcccggg ctgccgcctg tcgccacagc acgacgccgg cagtggcgcc
     3841 accggtcacc gagctcaagc ggacacgggc ggcaaaagcc cgttgctcct cggcgaccag
     3901 ctgccggatg aagccctcca gctgcagtgg tgcggtccgg gtgccgtcac cagcatgaag
     3961 cggcgccggc acgcggtggg cggccaggcg gtcgctgagc tgcgccagcc agtctgcgcg
     4021 cactcgctgg cgatgcgcct ccagcttggc gagcatgcgc ctgtccagcc ccgccagcgc
     4081 ctcctcccag tcgtccgccg ggaacagcag cgccgaggcc cggcgcgcga tgaagtcgcc
     4141 gtcggccagg ttgagcccgg cgagcgccgc cataccaagg cggctgtact caccgccgag
     4201 cgagtagtac cagtcggcaa attccggcag ccgggcgcgc accgcggcaa agacgtcttc
     4261 gagtccgccg tccagctcgg actccagcag ccgccgggct tccgcccgcc ctgcgctgaa
     4321 atgcaggacg gagaactcgc gcagccactc cagctgcggc gcatcgagcc ggtagtgctc
     4381 gccgtccagc accacctcgg tgggcgtggg cggcgcccag cgctgttgcg gctggcccag
     4441 catgagcacc agcaggccgg cggcggccac cacccacagc aacgccaaca gtcccgggcg
     4501 gggcaccgga cgaatcgggc gggtcgcttc agtggtcgct gcggtcaatg gcgcctgctc
     4561 ccagcagtgt tccgtggcac aacaacaggt atgaccacac aaacagcact tcttctgcaa
     4621 gtatcagcag ccagccgagc agttgcagca gggaagtcgc tggtgccggc atcagctgct
     4681 gggccagcca caggcgcacg ccgtccttcg ccgcggcggc ctgcagcaag acctcggccg
     4741 ggccgctgcg tgcccgctcc tggtcgacca tgtgccagac ggcgcgctcc aggctgaccc
     4801 cggcgaagtc cggataggcc tgctggaacc ccagccacgc cagcacggcc atgagcgccg
     4861 caccgaccgg ccaggcggcc gcacgccagc tcaggatggg caggtacagt gcgcccgcat
     4921 gtccttgcag gcgccgctgg agcctggcgc gcagcagcag cagcgcgggc ccggatgcca
     4981 tcagcgccac ccaggcccag tgatcgctgg tgcgcgcgag ggccaccatg agcaggattg
     5041 cgagcagccc gcccagcagc aagggacgca aggccagcaa cgggccaccg cggagcaggc
     5101 gctgcagggg actcgccgcg gcgatgtaga cggcgaggaa ggcgcgacgg cggatccgtg
     5161 cccgctgcca cagggccgtg gcgatgagcg tcgcgaacag cgcccatgtg gccgccagcc
     5221 caatgctcgg cagcacccgc gcgaacacat ggtagagcca tgccagggcg acgaggccca
     5281 gcagacacag caggtcacgc caaagccggg cagcgtccgg catggtcgac ctattgcgcc
     5341 agccagcgcc cgacctgcgc gaacaccctt ggcgtcgaca gcaggtcgag gtggcccagg
     5401 cccgtcccga tccagcgatt cgcggccggg aaacgcagcg cgaagcgtgc gtcctcgtgt
     5461 tcacccagcg cactggccag cggcaccagc ccgtcgccga ccaggcgtgc cgccggcgta
     5521 tcgtcgcgct cagcacgaag cgccgcggcc gcgtagcacg cgacccccgc cggcaacggc
     5581 aaaggtgtgc gcgggtcctt ccacatcggc gcatagcgat cgcaagcctg ccagtcttcc
     5641 tcgagcagga tgccgtaacg caggtcggtg atgccgtggc tgcgggtccg gccaaggcgc
     5701 atgaacggtc gcgtgtacgg actccagccc agcagcgcgt caaacccatt gccggcccgc
     5761 tccagcggcg cgccgtgatg aggcgtgccg agaaagacca gcttgccgac ccgccgcggc
     5821 cattcatgcc ccgcggcctc gccgtggtga tacgcgctcc gtaccacgag cccgcccatg
     5881 ctgtgcccga cgatcgccag ctcctccagg ggcaccggcc aatgccccga gagagactcc
     5941 agcagggcgg cgagcgtccg gccgttggcg gcgatgtgca gcccagaatt gtagtgcagg
     6001 tgcaggctgg tgtagccaag ggcgtgaccg agctcggcga gatagtcccg cccagtgcgc
     6061 gcccattggc cgggatgcat gcagagtcca tgcgcctgga tcagcaccct ggggccaggt
     6121 gctggaattg tcctgcgaat tgcgcccgct tcgaggtgca gcggcacacc gtcgcgacgc
     6181 aggctcatcg tgatggccaa cgggttgccg gtcgccgcca ggtggtcgcc gagcacgccg
     6241 ttgagcaccg ccagcgcagt ctcgtgcgac gccggccgct cccgtgtccc gacgagcatg
     6301 gcactcgacg ccagcaatcg gtcgagcccg ccgccgacga gtcgcatccc gcctcccacg
     6361 caacggtaca ccaggccggt cacgccgcgg gttcgctggt tgctggatcc cggaatgagg
     6421 ccggccaggc cggcgatggt gccgtgcatg gcctcaacca ggtcggtgac ctggctgccg
     6481 gcgtcgacaa tcaggcggct tgcaccccgc agatcggccg cgaggttatg cggcggcatg
     6541 ctcatttgcc gagccgcctg gtcaacgcgg cttctgttcg tgcttcgggt cgcgcttcgg
     6601 tgacgcatcg tcacccttgc ggtagtcgcc gatgaccccc ttcagggggc acaccgagaa
     6661 tatcgcgcat ttcctgcagc ctgcggccag ggcaaccggg caaagggtca tttcggtact
     6721 cctcggtggg atgctgacca cccgagccta gtggctccgg tcaggcggca caatggccgg
     6781 gcggtggcgc cgctgccgcc gcgatcattt ccaaaaactc gatcttcggg ttccggcccg
     6841 ccagctgcgc gaccgccgca cggccgtctt cgctggctcg atacgcctcc cagtcctcgc
     6901 cgcgcgccca ctggaagaac tcgacgatcc ggccgccgtc ctcgctcagg taaatcatcg
     6961 acgaaaggaa tccgggatga aatcgcactc gcgcctcgac ccaggcggcg atttctcgcg
     7021 ccaggcgctc gctctccccc ggattgcagt cgaacacgca cagcacggtg aacgcgcctt
     7081 cattgatcgg caagtttctc aacgaacatc ctccccgttt tgctcagtaa cccgccgcgt
     7141 gcccgtcctt gcgcggctcc gaagcgccta cccagacctc gccgtccttg cggatcgcct
     7201 gatagccacc gtagccgccg tcgttgatca cttcgacgcg gtgaccgcgc ttctccagct
     7261 cctcgatcgc cgccgccgga aagccgcgct cgacgcgcac cacgccgccg tcgctcatgc
     7321 gctcgccggt cggctcactg gagccgtcgt ggcggatgcg cggcgcatcg cccgccgcct
     7381 gcaggtccat gccgaaatcg atcatgttca ccagcacctg cacatggccc tggggctgca
     7441 tgccgccgcc catcacacca aagctcagcc agggcttgcc ctcgcgggtg acaaaggccg
     7501 gaatgatggt gtgaaacggc cgcttgccgg gcgcgtagac gttcgggtgg ccgggctcga
     7561 ggctgaacat ctcgccgcgg tcctgcagca cgaagccgag ccccggcggg gtcatgcccg
     7621 agcccatgcc gcggtagttg ctctggatga gcgacaccat gttgccgtcg gcgtcggcaa
     7681 ccgtcaggta taccgtgtcg ctgcgctcga gtacctccgg gtcgccgtgc gggaacgcct
     7741 gtgctgccct ggccggatcg atcagcttgc gccgctcggc cgcgtaggcc ttcgaaatca
     7801 gcgcctcggt cgggacctcg gcgaactccg gatcggcgta gaagcgcgcc cggtcctcga
     7861 aggcgagttt cttcgcctcg acgaacaggt gcaggtaatc ggcgctgccg aagcccatgg
     7921 cgccgatgtc ccaggcctcg agaatgttga gcatctgcaa gccggcgatg ccctgcccgt
     7981 tgggcggcag ttgccagacg tcgtagccgc ggtagttcac cgccaccggg tcgatccact
     8041 cggagcgatg cgcggcgaga tcctcgtagg ccaggaagcc cccgttggcc tgcatgtagt
     8101 cggcgatgac gtgcgcgatc ttgcccttgt agaaagcgtc gcggccgtcc tcggcaaggc
     8161 gctcgtaggt atcggccagc gccgggttgc ggaagattta gcccttggcc ggcgcccgcc
     8221 caccgggcat gaacacttcc gcgaagccgg gatattcgcc gatccgctcg acgttgcgcg
     8281 cccagtagta agcgatgacc tcggtcagcg gaaatccctc gcgggcatag cggatggccg
     8341 gcgccagcac atctcgcatc ggcgcgcggc cgaagcggcc gtgcagttcg aaccagccgt
     8401 cgacggtgcc ggggacgctc accggcagcg gcccgtaggg cgggatgctc tggtagccgt
     8461 tgtcgaggaa ccactcgagc tccaggccgc gcggcgaacg accgctggcg ttcaggccgt
     8521 gcaacttgcc ggattccgca tcccagacga tggcgaacag gtcgccgccg atgccgcagc
     8581 cggtgggctc caccaggccc agcatggcat tggcggcgat ggccgcatcc accgcattac
     8641 cgcccgcctt gaggacgtcg agcgccacct gcgtcgccag gggatggctg gtggccgcca
     8701 tgccgtgcgt cgccagcacc tcggagcgcg tgacgaattc ggggccggtg atgcgatctg
     8761 cgctcaggga cacgccgtgg accagggtgg cggccagcag ggccaggccg ccatatattc
     8821 tacgagacat tgctccctcc cttcttcccg gcatggtgct ccgccgggcg accgctgtcg
     8881 acccgtgcgg cgccatgacc tcaatcgcgc gcccggaacc cgatctgccc gcgctcgtaa
     8941 tccaccgcga ccaccaggcc ccgcagtgct cccgcgccga acacaccgtc acgcccttgc
     9001 cgctgcccgc gcggcccgac cgccggacac ggcgcgacat cgatcgggat cgtccgaccg
     9061 gcgagcgtca ccacgccggg atccggctcc tcgtccaggc agatgccgac cggggcggca
     9121 ggctcgaact gtaaacgcaa ccaccgcgca gccggtccgc cggattccat cgagacttcg
     9181 aaatagggga ctcgctgcaa cactgcgagg cgatacacct cgggcatgcc gccgacgtcc
     9241 agcctgcccg cgcggtacac ggccaggcgg ctgttgccgg catccagctc gaccacgaag
     9301 cgccgcagca ggtcatagcc caataccccc gccgcggcgc cgccgggcgt gccgtgtggc
     9361 cactcctccg gcgccaccag caacaggctc tggccgtcca gcaccagcga acccaaggca
     9421 aagcggcgct gcttgatcag cagcccgccg cggatgcccg gcagcacgtc gtcccgcaag
     9481 gccagcgagc cggcgccgac cggtccgaag ccggacgcgc gcgcaccggt caatgccacc
     9541 gccccggccg acgcctgcag caggaacggc accggcggat tgtcatccac cgacacgggg
     9601 atccagatgc ggccatcgac ggtcgtctcc agcgccagtt cgaccgccgg cacgcccgac
     9661 ggccattcga tcggcgtacc gttgttggca tacgccagcc gcagcgtatc aatcctgtcg
     9721 cagccggcgg cgaccaggat tgccagcaac agtgccagac gtgcgctcag aacggaatcc
     9781 gctcgtcgtg aatcggaaac accacccgca cgctgtccgg ctcgcggctg cgcttgagct
     9841 ggtagccgga gtgatcgaag acctcgatca tcttggtgtt cgagggcagc atctcggcct
     9901 cgaactgctg gatgccgttg tcgcgcgcga tgcgtatcag gtgcttgagc aactgcgtgg
     9961 ccacgccccg cccctggtag tcgtcgcgca ccaggaaggc tacctcggcg cggcggggat
    10021 ccgtctccgg ctgcacctcg atgtagcgtc ccaccgcgat gatgccgtcc tcgtcgggcg
    10081 cttcggccgc aagcgccaca tgccggtaga aatccaggcg tgtgtagtag tcgagttcgg
    10141 cgtcggagag cgagttcttg gtctcgaagt agcggaaata gcgcgattgc tggcttgacc
    10201 gggtgaacag gttcagtatc gcgccgcggt cccactcgcg cagcgcgcgc aggaagaacg
    10261 gcgtcccgtc gatcatgtag ccgagggcgc gatactcacg atggtccggg atgctcatcg
    10321 gtcgccctct caggttttcg acgtcccgac cggcacccgg ccggtgggca cgcgcgccgc
    10381 ggcgcgctcg caatgcgctg cctggtcgtc aaagaaaatg tgcggccgaa aggcctcgag
    10441 gatctctgtc ttgggcacac cgcccaggaa aaatgcctcg tccaccacga taccccatgc
    10501 cagcagggtg cgtatgacgc gctcgtgcgc cggtccgccg cgggccgtga ccagcgcggt
    10561 gcggatcggc gtgatgccgt cgcgcgcagc cgcctgctgc agggccgaca gcttgtggag
    10621 cagtcgtgcg aacggcccct cggccagggg ctcgagcgcc cgcgcctgct catggcgcgc
    10681 aaaggcctcc agcccatcag cctggtagat acgctcggat tcatccgaga acaggatcgc
    10741 atcgccgtcg aaggcgatgc gaatctcgtc cgcggcctcc agcgggttct ccgggcgatc
    10801 atagaggagc gcggcggcca cgccggcgtc cagcgcacgg cggacgtcgt cctcgtagca
    10861 ggacaggaac aggtccacct tgaaggcccg caggtagggc gcgagcggcg ccccgccgga
    10921 cagggcggcg cgcgtgatgt cgagaccgta gtggcggatg gagttgaaga tccgcaggct
    10981 ggtctcggtg gagttgcgcg acatcaccac gacctcggcg cggcgcgccg ccgccgggcc
    11041 gttcaaggcc agcacggccc tggcgagcgg aaagccggtg ccgggcggca ggacctggtc
    11101 ttcacgaccg agctgctcgg cccggtaggc gtccagcccg gcggagacga acagcgcatc
    11161 ctcgcggcgc aggtcgaaca gcgcgcgaga cgagatgccg acaacgaggc aagttgaaag
    11221 gtcggtcgtc acgggggcct ccgtgctcca ttatccgcgg tctcgactgt caccgcgacc
    11281 cgggacgtgc agggcggtgg cgctgctctc tcgagggccg cccgccagtt gccaatgctg
    11341 caggctcaaa acgaggaagg ccgggcacca ggcccggcct tccgctcatt gaaatgcggc
    11401 tctgaccgcg attattcggc ggtcttctcc ggctggtcga ccagctgcac cagcgccatg
    11461 ggcgcggaat cgccgggccg gttgccgatc ttgagaatcc gcaggtagcc gcccggccgg
    11521 gcccggtagc ggggcccgag ctcgttgaac agcttgccca cgacttcctt gtcccgcagc
    11581 cggtcgaagg cgcggcggcg ctgggccacg ccgtcctgct tggccagcgt gatcagcggc
    11641 tcgacgacct tgcgcagctc cttggccttc ggcagcgtgg tgcggatggt ctcgtgcagc
    11701 accagcgacg acgccatgtt gcgatacatg gcggtgcggt gcgggctctt gcggcctagc
    11761 tgccgccctg ctttgcgatg acgcatgact cgttatcctg ttgctgcggg cgcgctcagg
    11821 cgacccgctc gtcgtcgtgc cggaggctgg ccggcggcca gccgtcgagg cgcatgccga
    11881 gctgcaggcc gtggttttcc agcaccgcct tgatctcggt gagcgacttc ttgcccaggt
    11941 tcggagtgcg cagcagctcc acttccgtct tctggaccag gtcgccgatg tagttgatgc
    12001 tctccgcctt caggcagttg gccgagcgca ccgtcaattc cagctcgtcg accggcttga
    12061 gcaggatcgg gtcgatcgcc ttgtcgcccg gctccggccc gaggccttcg ccctgcaggt
    12121 ccacgaacac cgccagctgg tccttcagga tcgcgccggc gcgacggatc gcctcctccg
    12181 agtcgatcgt accgttggtc tcgatctcga tgatcagctt gtccaggtcg gtgcgctgct
    12241 cgacgcgggc ggagtcgacc gtgtaggcga cacggcgcac cgggctgaac gaggcatcca
    12301 gctgcagccg gccgatgggg ccgctctgct cttcgaaggc cggacgctgc gtgaccggct
    12361 ggtagccccg gccgcggcgc accttgaggc gcatcgacag gttgcccgcc ttggtcagcg
    12421 tggcgatgac caggtccgga ttgatgacct cgacgtcatg gccggtctcg atgtcggcgg
    12481 ccgtgaccac acccggcccc ttcttggaga ggttgagatc gatctcgtcg cgggaatgca
    12541 tcttgacggc cagctgcttg aggttcagca ggatgtcgac gacatcctcc tgcacgccct
    12601 cgatgctggt gtactcgtgc agcaccccgt cgatctcggc ctcgaccacg gcacagccgg
    12661 gcatcgacga cagcaggatg cgccgcaacg cgttgcccag cgtgtgaccg aaaccgcgct
    12721 cgagcggctc gatggtgact ttcgcgcggg tccggctctc cggctggacc ttgacgacgc
    12781 ggggacgtag aaattcgttg accgatgcct gcatgggctt cagtgcctcg caattacttg
    12841 gaataaagct cgacgaccag gttttcgttg atgtcgggca ggatgtcgtc gcgatccggc
    12901 agctgcttca gcacgccgct catcttggtc aggtccactt cgacccactc cgggaagccc
    12961 gcttccccgg ccagctgcag ggcgttctgc acccgcagct gcttcctggc cttgtcgcga
    13021 atcgcgatct cgtcgcccgg gttgacctgg tacgacggga tgttcacgac ctggccgttc
    13081 acgctgacgc ccttgtggcc gaccagctgg cgcgcctcgg cccgggtgga cgcgaagccc
    13141 atccggtacg cgacgttgtc caggcggccc tcgaggagct tcagcaggtt ctcgccggtc
    13201 gagcccttct gctgggcagc ccgcttgtag tagttgcgga actgccgctc cagcacgccg
    13261 tacatgcggc gcagcttctg cttttcacgc agctgcaggc cgtagtcaga aatacgcgga
    13321 cgacgctgcg gggcaccgcc gggcggcact tcgagtttgc acttggactc aagcggacga
    13381 acgctgctct tgaggaacag gtccgtgccc tcccgccggg agagcttgca ctttggtccg
    13441 agatatctag ccatattgat cgagcctctg cgaactagac gcggcgcttc ttgggcggcc
    13501 gacagccatt gtggggaatc ggggtcacgt cctcgatgtt cgtgacgcgg aatcccacgg
    13561 cattgagaga gcgtaccgcc gactcgcggc ccgggccggg gcccttgacg cgcacttcca
    13621 cgttcttgac gccgtattcc gcggcggcgt tgccggcctt ctcggcggca acctgcgcgg
    13681 cgaacggcgt gctcttccgc gagccgcgga aaccgcaacc gccggaagtc gcccaggaca
    13741 acgtgttgcc ctggcgatcc gtgatggtca cgatcgtgtt gttgaaggag gcgtggatat
    13801 gcgcaatcgc atccacgacc tgcttcttga tcttcttgcg caccttgggg gcttgcttgg
    13861 ccattcgttc gaaaccttca gcgttgg
//
LOCUS       DAOMPG010000041        13846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1900_length_13846_cov_4.408962, whole genome shotgun sequence.
ACCESSION   DAOMPG010000041 DAOMPG010000000
VERSION     DAOMPG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_13846_cov_4.408962"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..2602
                     /locus_tag="PLS34_03635"
     CDS             116..2602
                     /locus_tag="PLS34_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86595.1"
                     /translation="MRLYRALLGDPALRDAGPEWRDHWSYQLARAEAMAGREAEAEAV
                     LDALVVARPDSPLAPEAWFRVGEARFMARDWHAAERAYLATLELETDGPFSEQARYKL
                     GWTLFKQSLHEQSFAPFALVIDDRLAATPLDELDRAGRELVDDSFRAMAIGFAALEGA
                     ASVDAWLDGLESGDPEWAWLSYERLGELYVEQQRWTDAAAAYSAWATRAPRDGRAPLL
                     QARAIDALEEGGFAGEALAAMAAFADRFSLRGEWWQGRELAGHEAVRDRLKLSLDTLA
                     AHHHAVFQAGNGTAAAAAAWYRRWLDEFPAEPEAAERHFLLAELYYGDGALHEAAEAY
                     WSVAYGYGEHPRAAEAGYAAVVARRDLAALVPGDAGAELALVDAALTFSDAYPGEARA
                     PEVELEAAERLMNLGRLEPARAAAARVLARADAPAELRAAALLVSAHAAFDLEQYAAA
                     ESDYLAWRTETAVTGQPDATLAAGVAERIAASIYRQGQAAAAAGELAVAVGHYERIGA
                     AVPDSPVAATGQHDAAALLFNAAEWVQAAEAYETFLGRWPAHALAAEAQVSLAAALVE
                     LGDHARAAPALAAVAALPGETEVVRRAALWQAGELYQAGGDHAQAEEVLRQYLARFPV
                     PADAALEARHRLAELAGQRHDVVERRRWLESIITMHADAGGAATARSRTLAAAASLEL
                     AMPAVERFASLALGPPLERSVPEKAARMRVALDALGAAAAYGVAEVTTESAFRMAELY
                     RGMATALLESARPPGLDEDTLEQYEMLLEEQAFPFEEDAIEIHEANAARARDGLYDRW
                     VVASFEALAEMVPARWNRQETGEHVVELHR"
     gene            2586..3206
                     /locus_tag="PLS34_03640"
     CDS             2586..3206
                     /locus_tag="PLS34_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP86596.1"
                     /translation="MNCIASQGRPRDRGRQRWIPCTGALLLLLSGCAVSPDRPGQAPH
                     ADDRRFERAATAMQQGPQEDALELLDDLAADYPTLSGPWVNLGILHAAAGRDEDAEAA
                     FRRAIEVSPRHPVAHAELGIVLRRQGRFAEADEAYREALALDPDYALAWRNRGVLLDL
                     YLGRPAEALACYERYLELVGGTDADEQVARWVAELRLRSDSRVATR"
     gene            3203..3538
                     /locus_tag="PLS34_03645"
     CDS             3203..3538
                     /locus_tag="PLS34_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86597.1"
                     /translation="MNAVRPVAVLIALLLASSAGAEEQAKIELDTAKVTGNRELPRVM
                     AIVPWKKAPAGELPGRPVQSLLDEALAPADRLVMRRQSSQWAALEAARQNEVSGPSGT
                     RRASSIVEE"
     gene            3542..4204
                     /locus_tag="PLS34_03650"
     CDS             3542..4204
                     /locus_tag="PLS34_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP86598.1"
                     /translation="MDVYTAIVGFFQQGGVFMYPIVIVLVLGTAIAVERYVYLSAAGV
                     SNRKLWQQLMPMVKSGNYAEAMNVAGRSKVAIGNILAYGLSRLSTARRRDDVEKAMEE
                     SLLETLPRIEKRTHYLATFANIATLLGLLGTIIGLIKAFTAVAAANPAEKADMLSASI
                     SVAMNTTAFGLMVAIPLLLAHTVLQSKTTEIVDSLEMAAVKFLNAVTDPAPSPQGKAT
                     GG"
     gene            4206..4706
                     /locus_tag="PLS34_03655"
     CDS             4206..4706
                     /locus_tag="PLS34_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP86599.1"
                     /translation="MRLLKRRRRIREPGELDITAFMNLMVVLVPFLLITAVFSRIAVH
                     ELTLPGPSSQTTEAAEQGLNLEVVVREDALEVGDRRTGLLRRFEADGEGIFDYAALSE
                     FLAQVKLRHPEAREAMILLEPEIHYQRLVDVMDTLRVARVEQGTAELFPDISIGDAPA
                     RTGGRK"
     gene            4703..5197
                     /locus_tag="PLS34_03660"
     CDS             4703..5197
                     /locus_tag="PLS34_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP86600.1"
                     /translation="MNGSRRARRMDAHHKRRRGAPLSLVSLMDIFTILVFFLLVNSSD
                     VQQLTTPKSLQMPESIAQQPPRETVVVTVGPDAILVQGEVVAITADVLDGQDDFIPAL
                     AAALEQLSARRLRVDDGLPPEVTILGDRDLPYRLLRKVMVTCTRADYGRVSLAVVQRG
                     AESA"
     gene            5208..6152
                     /locus_tag="PLS34_03665"
     CDS             5208..6152
                     /locus_tag="PLS34_03665"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HRP86601.1"
                     /translation="MTTIAIRGLDLPWTLDPVVEQRFRRILKRTALVALLLSLLVPWL
                     PIPERDASLPPEVPERYARLLIERTPPPPPPVVAVEPEPEPVVEQAAAKPEPVETPKP
                     RPEEPPRPSARERAASTGVMAFADALADLRSNDAVAAATTADGLAAGAGETQHNERNV
                     VTARGGRASGGINTAGMSRNTGGTGLGGRAVTQVSSPVGGGGGGGGGGGASGTGGDGL
                     NARSREEIELVFDRNKAAIYALYNRALRSDPTLRGRLVLELTIAPSGEVTGCKVVSSE
                     LSDPEFERRLVARVMMFRFEDKDVATVTTTKPIEFFPA"
     gene            6152..6793
                     /locus_tag="PLS34_03670"
     CDS             6152..6793
                     /locus_tag="PLS34_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86602.1"
                     /translation="MAAAPRTRVRLFACVLALAAANGPGAAEPARLAATLVTPEGAAA
                     LLDFGAGQAWLRAGESMGGCQMESVATAAAELRCGDRRIRLALEPGLQPAAESVPAPA
                     PEFGQAALPPGMLRALAERPQAIALAADFAPVVEQGRLQGWRVARLDEGGPLAGMGLR
                     EADVIHAIDGAPAAEPAAFAAAIRALPGVRAFTLELSRDDRPLTLLVAAPPAP"
     gene            6944..7780
                     /locus_tag="PLS34_03675"
     CDS             6944..7780
                     /locus_tag="PLS34_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP86603.1"
                     /translation="MEEISSLVTPEQVEALVEGTSKFGLQIVAALVVFFVGRWLARRI
                     SNLVERGLTRAGTDPTLIGFFHNIAYFGLLVLVIIAAVGQLGVQTTSFIAVIGAAGLA
                     VGLALQGSLANFAAGVLMIIFRPFKAGDFIEAAGTGGVVEEIQLFTTKMRTPDNKTII
                     IPNAQITAGTITNYSARDTRRLDLVFGVSYADDLDKVKRVINEVLAEEKRLLDDPEPL
                     VGVLKLGDSSVDFAVRPWVNSSDYWAVFFDLQEAMKKRFDREGISIPFPQRDVHLYQQ
                     GS"
     gene            7812..10943
                     /locus_tag="PLS34_03680"
     CDS             7812..10943
                     /locus_tag="PLS34_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP86604.1"
                     /translation="MRRSWPILRLWLVQALLFSIPVILLLAWVISERREDHLREAHAR
                     AAILAESYARELGLLVRDGADLLRTFRDLLPLRAVSPEGCPAGGTQGLLDSSRRYGNL
                     LLVDAGGQVLCAAAGQAGGQAGFADRDWFRRTIQGSGLQVSGLVRGRLTGRDIVVISI
                     PVRDSPSEPPRVLALTLDLLTLSESLARDSLPDAAVVSLVDDQRVVLARTLGPQQWIG
                     ERVELANYPAEVSPGQVETHAGLDGVVRLYTAARVAEMPWTVFVGLPADDVEAVIRAD
                     LQRTALVVGIAALLLLLLAIWMSASVGTPVRRLAAALREVRLKGGGLPRGLQGPRELE
                     EVVQEFDRLVARQAETLEQVERSERRYRATFEQAPVGIVHQDETGRYVRVNVRFAEMV
                     GRDPEELLGMHDHQLTHPDDRPSSGAIYRRLLAGDVRFGNLQKRYLRMDGKEVWTDVV
                     ISRVGEADEEPLLISVIEDVSDSREESERRALAATVFESSTEGIIITDAARRIRAINR
                     AFVELTGFGERDIIGQPVEALQTDEHAQDFYQSIWSGVRRDAQWRGEIWLPQKAGGKF
                     PALMNLTEVRDARERITSYVAVFADISHEKRTEAELDYLVHHDALTKLPNRRLFHARL
                     EHTIQQAYRDPQSHFSLLFIDLDDFKVVNDSLGHAAGDQLLIELARRLSASVRAEDTV
                     ARMGGDEFMVLLDGAGTFNEINKVVGKLLAAAQQPFQIGPHEVFVSASIGVAIFPLDA
                     EDGATLARNADVAMYRAKDKGKNRFEFYEASMSEQAMERLSLANDMRRALEGSQFYLV
                     YQPKVGALDGRILGFEALLRWRHPERGEISAQRFIKVAEETGLIVPIGVEVLGMATRT
                     MAEWRARGLEGVTMAINLSAQELAHPRLFDRVREALAASGLPGAALEIEITESALIRD
                     PRHVAPLLRKLRALGLRIAIDDFGTGYSSLNYLRQFRVDVLKIDQSFIRELENSSRAR
                     IIPQAVVALGQALQMSTIAEGVETESQAAILREMGCDQLQGYGVGRPLPRAEAERMLA
                     AHGLLAAASPPGG"
     gene            complement(10976..11052)
                     /locus_tag="PLS34_03685"
     tRNA            complement(10976..11052)
                     /locus_tag="PLS34_03685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11016..11018),aa:Pro,seq:cgg)
     gene            complement(11077..12093)
                     /gene="ispB"
                     /locus_tag="PLS34_03690"
     CDS             complement(11077..12093)
                     /gene="ispB"
                     /locus_tag="PLS34_03690"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HRP86605.1"
                     /translation="MPTPLTSARPAEAAGRLSLSEIRDLAARDLDSLDRWIVASLDSG
                     VMLVNQVARHIVAGGGKRLRPLLVLLAARACGYQGDRHIGVAAVVEFIHTATLLHDDV
                     VDGSDLRRGRDTANAVFGNEASVLVGDFLYSRAFQMMVGMGQLRVMEIMADATNVIAE
                     GEVLQLMNCHDPETDESRYFDVIRCKTAKLFEAGARVAAVLAGSDEATEAALADYGMR
                     LGIAFQLVDDALDYSGAVEEMGKNMGDDLAEGKPTLPLIQAMREGSPEQRETIRRAIE
                     HGGLEELAAIRDAIESTGALNYTARLAREQAEAAVEALAALPESVHRDALASLARIAV
                     ERTA"
     gene            12277..12591
                     /gene="rplU"
                     /locus_tag="PLS34_03695"
     CDS             12277..12591
                     /gene="rplU"
                     /locus_tag="PLS34_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRP86606.1"
                     /translation="MYAVIATGGKQYRVSEGAVLRVEKLDADEGASIEFDRVLLVGDG
                     DSVSVGKPFLDGSKVQATVMSQGKARKVEVLKFRRRKNYRRTKGHRQNFTQVKITGIV
                     AG"
     gene            12618..12875
                     /gene="rpmA"
                     /locus_tag="PLS34_03700"
     CDS             12618..12875
                     /gene="rpmA"
                     /locus_tag="PLS34_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRP86607.1"
                     /translation="MAHKKAGGSTRNGRDSQSKRLGVKKYGGEQVLAGNIIVRQRGTR
                     FHPGVNVGCGRDYTLFAKADGRVLFETKGPHNRKFVSIVTD"
     gene            12994..>13846
                     /gene="obgE"
                     /locus_tag="PLS34_03705"
     CDS             12994..>13846
                     /gene="obgE"
                     /locus_tag="PLS34_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRP86608.1"
                     /translation="MKFVDEATITVQAGAGGNGCISFRREKFIPFGGPDGGDGGNGGS
                     VWLVADAGLNTLADFRRQRRFRAANGAPGMGSNCSGRGGESKEIMVPVGTVVHEESTG
                     EILGDLVEPEQRLLVAQGGIGGKGNTHFKSSVNRAPRKSTPGTPGELRHLRLELRLLA
                     DVGLLGLPNAGKSTLLRAVTAARPKVGDYPFTTLHPNLGVVDLEEGKTFVIADIPGLI
                     EGAAEGHGLGTRFLRHLQRTRLLLHVVDASQHEGEPPPEQGARDIIHELKSYSAELAS
                     WPRWLVIN"
BASE COUNT         2135 a         4612 c         5021 g         2078 t
ORIGIN      
        1 ggaacagctc gttggtgacg acgtcggcga ccaggcccgc gttcgggccg ttgatgtcgt
       61 cgcgccagcg cacgccgacg tcgcgcagcg caccgcggcg cacctcgacg aggccgtgcg
      121 tctgtatcgg gccctgctgg gtgaccctgc gctacgcgac gccggccccg agtggcgcga
      181 tcactggagt taccagctgg cgcgcgccga ggccatggcc ggacgcgaag ccgaggcgga
      241 ggcagtgctg gatgcgctcg tagtggcgcg tcccgactcg ccgctggcgc cggaggcgtg
      301 gtttcgcgtc ggcgaggcgc gcttcatggc gcgcgactgg cacgcggcgg agcgtgccta
      361 cctcgccacg ctggaactcg agaccgacgg gccgttttcg gagcaggccc gctacaagct
      421 gggctggacg ctgttcaagc agtcgctgca cgaacagagt ttcgcgccct tcgccctggt
      481 catcgacgac cggctggccg caacgccgct cgacgaactg gatcgggccg gccgggagct
      541 ggtcgacgac agcttccggg cgatggcgat cggctttgcc gccctcgagg gtgcggcatc
      601 ggtggatgcc tggctggacg gcctggaatc cggggatccg gagtgggcct ggctaagcta
      661 cgagcggctc ggtgaactgt acgtggaaca gcagcgctgg acggatgcag ctgctgccta
      721 cagcgcctgg gcgacgcgcg cgccgcggga cgggcgtgcg ccgctgctgc aggcacgcgc
      781 catcgacgcg ctcgaggagg ggggcttcgc gggtgaggcg ttggcggcga tggcggcatt
      841 tgcggaccgg ttttcgctgc gtggcgaatg gtggcagggc cgcgaactgg ccggacacga
      901 ggccgtgcgc gatcgcctga aactcagcct ggacaccctg gcggcgcatc accatgcggt
      961 cttccaggcc ggcaacggca ccgctgcggc ggccgccgcc tggtaccggc gctggctgga
     1021 cgaattcccc gcggagcccg aggccgccga gcggcacttc ctgctcgcgg agttgtatta
     1081 cggcgacggc gccctgcatg aggctgcgga agcgtactgg tcggtggcct atggctatgg
     1141 cgagcacccg cgtgcggccg aggcaggcta cgccgcggtg gtcgcccggc gcgacctggc
     1201 ggcgctggta ccgggtgacg ccggcgccga gctggcgctg gtggacgccg cgctgacctt
     1261 ttccgatgcg tatcccgggg aggcgcgcgc ccccgaagtg gagctcgagg ccgccgagcg
     1321 gctgatgaac ctgggccgtc tcgagccggc gcgcgccgcc gccgcgcggg tgctggcgcg
     1381 cgcggacgcg ccggcggaat tgcgtgccgc ggccctgctg gtgagcgcgc acgcggcgtt
     1441 cgacctggag caatatgccg ccgcagaaag cgactacctc gcctggcgca ccgagaccgc
     1501 cgtcaccggg cagccggatg cgacgctggc ggccggcgtc gccgaacgca tcgccgcgag
     1561 catctaccgc cagggccagg cggccgccgc ggccggcgag ctggcggtcg cggtcggaca
     1621 ttacgagcgt atcggcgcgg ccgtgccgga cagcccggtg gccgcgaccg gccagcatga
     1681 cgcggcggct ttgctgttca acgccgccga atgggtgcag gcggcggagg catacgagac
     1741 attcctcggc cgctggcccg cacacgcgct cgcggccgaa gcacaggtga gcctggccgc
     1801 cgcactggtc gaactcgggg accatgcccg cgcggcgccg gcgctggccg cggttgcagc
     1861 gctgccgggc gagaccgaag tcgttcgtcg cgcggccttg tggcaggcgg gcgagctcta
     1921 ccaggcaggc ggggaccatg cgcaggcgga agaggttctc cgccagtacc tggcgcgttt
     1981 cccggtgccg gcggacgcgg cgctcgaggc gcgccaccgc ctcgcggaac tcgccggaca
     2041 gcgccatgac gtcgtcgagc ggcggcgctg gctggaatcg atcatcacga tgcatgcaga
     2101 cgccggcggc gcggccaccg cccgcagccg gacgctcgcc gcggccgctt cgctggagtt
     2161 ggccatgccc gcggtcgagc gctttgcctc gctggcgctc gggccgccgc tggagcgcag
     2221 cgtgccggag aaggcggcgc gcatgcgcgt ggcgctcgac gcgctcggcg ctgcggccgc
     2281 gtacggcgtc gcggaggtca cgaccgagtc ggccttccgc atggctgaat tgtaccgggg
     2341 catggccaca gcgctgctgg agtcggcgcg tccgccgggc ctcgacgagg acacgctgga
     2401 acagtacgag atgctgctcg aggagcaggc ctttcccttc gaagaggacg ccatcgaaat
     2461 tcacgaggcg aatgcggcgc gcgcccgcga cggactgtat gaccgctggg tggtcgcgag
     2521 tttcgaggcg ctggccgaga tggtgccggc gagatggaac cgccaggaga caggagaaca
     2581 tgtcgttgaa ctgcatcgct agccaggggc ggccgcgtga ccgcggacgg caaaggtgga
     2641 ttccctgcac cggcgccttg ctgttgctgt tgtcgggatg cgccgtgtcg cccgaccggc
     2701 cggggcaggc gccacatgcg gacgaccggc gcttcgagcg cgcggcgacc gccatgcagc
     2761 agggaccgca ggaggacgcc ctcgaactgc tcgacgacct ggcggccgat tacccgacgc
     2821 tgtccgggcc gtgggtcaat ctcggcatcc tgcatgccgc cgcgggtcgg gacgaggacg
     2881 ccgaagcggc cttccggcgc gccatcgagg tatcgccgcg acacccggtg gcgcatgccg
     2941 agctgggcat cgtactgcga cgccagggac ggttcgcgga ggccgacgag gcctaccgcg
     3001 aggcgctggc gctcgacccg gactatgccc tcgcctggcg caaccgcggg gttctgctcg
     3061 acctgtacct cgggcggccg gccgaggcgc tggcgtgcta cgagcgctat ctcgagctgg
     3121 tcggcgggac ggacgccgac gagcaggtag cgcgctgggt ggccgagttg cgcctgcgca
     3181 gcgactcgcg ggtggcgacg cgatgaacgc cgtgcgcccc gtcgccgtgc tcatcgccct
     3241 gctgctggcg tcgtcggcag gggccgagga acaggcgaag atcgagctgg acactgccaa
     3301 ggtgaccggc aatcgcgagc tgccgcgcgt catggcgatc gtgccgtgga agaaggcgcc
     3361 ggccggcgaa ctgccgggcc ggccggtgca gagcctgctc gacgaggcgc tggcaccggc
     3421 cgaccgcctg gtgatgcggc gccagtcgag ccagtgggcc gcactcgagg cggcccggca
     3481 gaacgaagtt tcagggccgt ccggcacgcg ccgggcgtcg tcaatcgtgg aggagtgagt
     3541 aatggacgtt tacaccgcaa tagtcggttt ctttcagcag ggcggggtct tcatgtaccc
     3601 catcgtgatc gtgctggtgc tcggcaccgc cattgccgtc gagcgctacg tttacctgtc
     3661 ggccgccggc gtcagcaacc gcaagctctg gcagcagctg atgcccatgg tgaaatcggg
     3721 caactacgcc gaggccatga acgtggccgg ccgctccaag gtcgccatcg gcaatatcct
     3781 ggcctacggc ctgtcacgac tcagcacggc gcgccgccgc gatgacgtcg agaaggccat
     3841 ggaggaaagc ctcctggaga cgttgccgcg catcgagaag cgcacgcact acctggcgac
     3901 ttttgccaac atcgcgacgc tgctcggtct gctcggcacc atcatcggcc tcatcaaggc
     3961 gttcaccgcg gtggcggcgg ccaacccggc cgagaaggcc gacatgctgt cggcgagcat
     4021 ctcggtggcc atgaacacca cggccttcgg gctgatggtc gccattccgc tgctgctggc
     4081 gcacacggta ctgcagagca agaccaccga gatcgtcgac agcctggaaa tggcggcggt
     4141 gaagttcctc aacgccgtca ccgatccggc gccgtcgccg cagggcaagg cgacgggagg
     4201 ctgacatgag actcctgaag cgtcgccgcc ggatccggga gccgggcgag ctggacatca
     4261 ccgccttcat gaacctgatg gtggtgctgg tgccgttcct gctgatcacg gccgtgttct
     4321 cgcggatcgc ggtgcacgag ctgaccctgc ccggcccgtc gtcccagacc accgaggcgg
     4381 ccgagcaagg cctgaacctc gaggtcgtgg tgcgcgagga cgcgctcgag gtcggcgacc
     4441 ggcgcacggg gctgctgcga cgcttcgagg cggacggcga gggcatcttc gactacgcgg
     4501 cgctgtcgga attcctggcg caggtgaagc tgcgccatcc ggaggcccgc gaggcgatga
     4561 tcctgctgga gccggagatt cactaccagc gcctggtcga cgtcatggat acgctgcggg
     4621 tggcgcgcgt cgagcaaggg acggcggagc tgtttccgga catctcgatc ggcgacgcgc
     4681 cggcacgcac cggaggccgc aaatgaacgg atcaagacgc gcgcgtcgca tggacgccca
     4741 ccacaagcgc cggcgcggag cgccgctgag cctcgtgtcg ctgatggaca tcttcaccat
     4801 cctggtgttc ttcctgctcg tcaactcctc cgacgtgcag cagctcacca cgccgaaatc
     4861 gttgcagatg cccgagtcga tcgcccagca accgccgcgc gagaccgtcg tcgtgaccgt
     4921 gggcccggat gcgatcctgg tgcagggtga agtggtggcg atcaccgccg acgtactcga
     4981 cggccaggac gacttcattc ccgcgctggc ggcggcgctg gaacagctca gcgcgcgccg
     5041 cctccgggtg gacgacggcc tgccgcccga agtgacgatc ctgggcgacc gcgacctgcc
     5101 ttaccggctg ttgcgcaagg tcatggtgac ctgcacccgc gccgactacg ggcgcgtctc
     5161 gctggccgtc gtgcagcgcg gcgcggaaag cgcatgagga ggccggcatg accaccatcg
     5221 cgatacgcgg tctcgatctg ccctggacgc tcgacccggt ggtcgagcag cgcttccgcc
     5281 ggatcctgaa gcggacggcg ctggtcgccc tgctgctgag cctgctggtg ccctggctgc
     5341 cgatccccga gcgggacgcg tcgctgccgc cggaggtgcc ggagcgctac gcgcggctgc
     5401 tcatcgagcg cactccgccg ccaccgccgc cggtggtcgc ggttgagccg gagcccgaac
     5461 cggtcgtcga acaggcggcc gccaagccgg agccggtcga gacgcccaag ccgcgacccg
     5521 aggaaccgcc ccggccgagc gcgcgcgaac gcgccgcgtc gaccggcgtg atggcctttg
     5581 ccgacgccct ggccgacctg cgcagcaacg atgcggttgc cgcagccacc acggccgacg
     5641 gcctcgcggc gggcgctggc gagacgcagc acaatgagcg caacgtggtc actgcgcgcg
     5701 gtggacgggc cagcggcggc atcaacaccg ccggcatgtc gcgcaacacg ggcggcaccg
     5761 ggctcggcgg ccgtgcggtc acgcaggtgt cgtcgccggt cgggggtggt ggcggtggtg
     5821 gcggcggcgg cggtgcgtcc ggaaccggtg gcgacggcct caacgcgcgc agtcgcgaag
     5881 agatcgaact ggtcttcgac cgcaacaagg cggcgatcta cgcgctctac aaccgtgcgc
     5941 tacgcagcga tcccacgctg cgtggaaggc tggtgctgga attgacgatt gcacccagcg
     6001 gcgaagtgac gggttgcaag gtcgtgtcgt ccgaattgtc ggacccggaa ttcgagcggc
     6061 gcctggtggc gcgcgtgatg atgttccgct tcgaggacaa ggacgtcgcc acggtgacca
     6121 cgaccaagcc gatcgagttc ttcccggcct gatggctgcc gcgccacgca ctcgtgtgag
     6181 gctgttcgcc tgcgtactgg cgctggcggc cgcgaacggc cccggtgccg ccgagccggc
     6241 ccgcctggcg gcgaccctgg tgacgccgga gggcgcggcc gccctgctcg atttcggcgc
     6301 cggacaggcc tggcttcgtg cgggggagtc gatgggagga tgccagatgg aaagcgtcgc
     6361 gactgcagcc gccgagctgc gctgcggcga ccggcggatc cggctcgcgc tcgagccggg
     6421 tctgcagccg gccgcggagt ccgtgccggc gccggcgccc gaattcggcc aggcagcact
     6481 cccgccaggc atgcttcgtg cgctggccga gcggccccag gccatcgcgc tggccgccga
     6541 tttcgcgccg gtggtcgagc aggggcgcct gcagggctgg cgcgttgcgc ggctggatga
     6601 aggcgggccg ctggccggga tggggctgcg cgaggccgac gtgatccacg cgatcgacgg
     6661 cgcgccggcc gcggagccgg ctgcgtttgc cgccgccata cgggcgttgc cgggcgtccg
     6721 ggccttcacg ctcgaactgt ccagggacga ccggccgctg acgctgctgg tggctgcgcc
     6781 acccgccccc tgacgcagac cacccatgct cgacatcatc cggccgtcga acctccgggt
     6841 tgctgcgcgc ggcgcccgtc gttttgcgcg ccggtgacaa tgacggcaga ctatggcgac
     6901 ttttcacggg gcgtggtcgc ccgcggaaca aggagaatca aggatggaag agatcagctc
     6961 gctggtcacc ccggagcagg tcgaggcgct ggtggagggc acctcgaagt ttggcctgca
     7021 gatcgtcgcc gccctggtcg tgtttttcgt cggccgctgg ctggcacgac gcatcagcaa
     7081 tctcgtagag cgtggcctga cgcgcgccgg cacggacccg acgctgatcg gtttcttcca
     7141 caacatcgcc tatttcggcc tgctggtgct ggtgatcatc gcagcggtcg gccagctggg
     7201 cgtgcaaacg acctcgttca tcgcggtgat cggtgccgcc ggccttgccg tcggcctggc
     7261 gctgcagggt tccctcgcga acttcgccgc cggggtactg atgatcatct ttcgcccctt
     7321 caaggccggt gatttcatcg aggccgccgg cacgggcggg gtcgtcgagg aaatccagct
     7381 gttcacgacc aagatgcgga cgcccgacaa caagaccatc atcattccca acgcccagat
     7441 cacggccggg accatcacca attactcggc gcgcgacacg cggcgcctgg acctggtgtt
     7501 cggcgtgagc tacgcggacg atctcgacaa ggtgaagcgc gtgatcaacg aggtgctcgc
     7561 cgaggagaag cggctgctgg atgatccaga gccgctcgtc ggggtactca agctcggcga
     7621 cagcagcgtg gatttcgcgg tgcgaccgtg ggtgaacagc agcgactact gggcggtatt
     7681 cttcgacctg caggaggcca tgaagaagcg cttcgaccgc gaaggcatca gcatcccgtt
     7741 cccgcaacgc gacgtgcacc tttaccagca gggctcctga cgcaggacgc aatggggcct
     7801 gtgcgagcaa tatgaggcgt tcctggccga tcctgcgtct ctggcttgtc caggccctgc
     7861 tgttttcgat tcccgtcatc ctgctgctgg catgggtgat cagcgagcgg cgggaggatc
     7921 acctgcgcga agcgcatgcg cgtgcggcga tactcgccga gagctatgcg cgcgaactcg
     7981 gcctgctggt gcgcgacggc gccgacctgc tgagaacgtt cagggacctc ctcccgctgc
     8041 gcgccgtgtc cccggagggc tgcccggctg gcggcacgca ggggctgctc gattcatccc
     8101 ggcgatacgg caacctgctg ctggtggacg ctggcggcca agtgctgtgc gccgccgccg
     8161 gccaggcggg cgggcaggcc ggtttcgccg atcgcgactg gtttcgtcgc accatccagg
     8221 gctccggtct gcaggtcagc ggcctcgtcc gggggcgcct gaccggccgc gacatcgtgg
     8281 tgatctcgat tccggtacgg gattcaccga gcgagccgcc aagggtcctg gcgttgacgc
     8341 tcgacctgct cacgctgtcg gagagtctcg cccgggactc gctgccggac gcggcagtgg
     8401 tgagcctggt ggacgaccag cgcgtggtgc tggcgcggac cctcggccca cagcagtgga
     8461 tcggcgagcg ggtggagctg gcgaactacc cggccgaggt gtctcccggg caggtggaga
     8521 cgcatgcggg gctggacggc gtggtgcgtc tctataccgc ggcacgggtg gcggagatgc
     8581 cctggaccgt gttcgtgggg ctgcccgccg acgacgtcga ggccgtaatc cgggcggacc
     8641 tgcagcggac cgcgctggtg gtcggcattg cggcgctgtt gttgctgctg ctcgcgatct
     8701 ggatgagcgc cagtgtcggt acgccggtgc gccggctggc ggcggcgctg cgcgaagtgc
     8761 ggctcaaggg cggaggcctg ccgcgggggc tgcagggacc gcgcgaactc gaggaagtgg
     8821 tgcaagagtt cgatcggctg gtggccaggc aggcggagac gctcgagcag gtggaacgca
     8881 gcgagcggcg ttaccgcgcc acgttcgagc aggcgcccgt cggcatcgtg caccaggacg
     8941 agaccggccg ctacgtacgc gtcaacgtgc gctttgccga aatggtggga cgcgaccccg
     9001 aggagctgct gggcatgcat gaccaccagc tcacgcaccc cgacgaccga ccttccagcg
     9061 gcgccatcta tcgtcgcctg ctggccggcg acgtccgctt cggtaacctc cagaagcgct
     9121 acctgcgcat ggacggcaag gaggtctgga ccgacgtagt gatctcgcgg gtcggcgagg
     9181 ccgacgagga accgctgctg atcagcgtga tcgaggacgt cagcgactcc agggaggaaa
     9241 gcgagcgccg ggcactcgcc gccaccgtgt tcgagagctc caccgagggc atcatcatca
     9301 ccgacgcggc gcgccgcatt cgtgccatca accgggcctt cgtcgagctg accggctttg
     9361 gcgaacgcga catcatcggt cagccggtcg aagccctgca gaccgacgaa catgcgcagg
     9421 atttctacca gagcatctgg tccggcgtgc gccgcgatgc tcaatggcgg ggcgagatct
     9481 ggctgccgca gaaggccggc ggcaagtttc ccgccttgat gaacctcacc gaggtgcgcg
     9541 acgcgcgcga gcggatcacc agctacgtcg cagtgttcgc cgacatatca cacgagaaac
     9601 ggaccgaggc ggagctcgac tacctggtgc accacgatgc gttgaccaag ctgcccaacc
     9661 ggcggctgtt ccacgcccgg ctggagcaca ccatccagca ggcctatcgt gatccacaga
     9721 gtcacttcag cctgttgttc atcgatctcg acgacttcaa ggtggtgaac gactcgctgg
     9781 gccacgcggc cggggaccag ttgctgatcg agctcgcgcg gcggctgtcc gcctcagtgc
     9841 gcgccgagga caccgtggcc cggatgggcg gcgacgagtt catggtgctg ctggacgggg
     9901 ccggcacctt caacgagatc aacaaggtgg tcggcaagct gctggcggcc gcccagcagc
     9961 cgttccagat cggcccgcac gaggtgttcg tctcggccag tatcggggtg gcgatcttcc
    10021 cgctggacgc ggaggacggc gcgacgctgg cgcgcaacgc ggacgtcgcc atgtaccgtg
    10081 ccaaggacaa gggcaagaac cgcttcgagt tctacgaggc gtcgatgtcc gagcaggcca
    10141 tggagcggct gagcctggcg aacgacatgc gtcgcgcgct ggagggcagt cagttctatc
    10201 tcgtgtatca gcccaaggtg ggcgccctgg acgggcgcat cctgggcttc gaggccctgc
    10261 tgcgctggcg ccatccggag cgcggcgaga tcagtgcgca gcgcttcatc aaggtagccg
    10321 aggaaaccgg cctgatcgtg ccgatcggcg tggaggtcct cgggatggcc acgcgcacga
    10381 tggcggagtg gcgcgcccgg ggactcgagg gcgtcaccat ggcgatcaat ctctcggcgc
    10441 aggagctcgc ccacccgcgc ctcttcgacc gggtgcgaga ggcgctggca gcgagcgggc
    10501 tgccaggcgc cgcgctggag atcgagatca ccgaaagcgc cctcatcagg gacccgcggc
    10561 acgtcgcccc actgttgcgc aagctgcgcg cactcggtct gcgcatcgcg atcgacgatt
    10621 tcggcaccgg ctattcctcg ctgaattacc tgcgccagtt ccgggtggac gtgctcaaga
    10681 tcgaccagag cttcatcagg gagctcgaga attcctcgcg cgcgcggatc attccccagg
    10741 ccgtggtcgc cctcggccag gccctgcaga tgagtaccat cgcggagggc gtcgagacgg
    10801 agtcgcaggc ggcgatcctg cgggaaatgg gctgcgacca gctgcagggc tacggggtgg
    10861 ggcggccgct gccgcgcgcg gaagccgagc ggatgctggc tgcgcacggt ctgctggcgg
    10921 cggcgtcgcc gcctgggggg taattcaggg atccaaccgc ggcgcaatgg ccgggtggtc
    10981 ggggtgagag gattcgaacc tccgaccttc tgctcccgaa gcagacgcgc taccaggctg
    11041 cgctacaccc cgtgcacggt ccggcgaccg tatcaatcag gccgttcgct cgacggcaat
    11101 gcgcgccagg gaggcgaggg cgtcccggtg gaccgattcg ggcagtgccg ccagtgcttc
    11161 caccgcggcc tcggcctgct cgcgcgcaag ccgcgcagtg tagttgagcg ccccggtcga
    11221 ttcaatagcg tcacggatgg ccgccagttc ctccagcccc ccgtgctcga tggcgcggcg
    11281 gatggtctcg cgctgctccg gcgagccctc gcgcatggcc tggatgagcg gcagggtggg
    11341 cttgccctcg gcgaggtcgt cgcccatgtt cttgcccatc tcctcgaccg cgccgctgta
    11401 atcgagggca tcgtcgacca gctggaaagc gatgcccagc cgcatgccgt agtcggccag
    11461 cgcggcctcg gtcgcctcgt ccgagcctgc cagcacggcc gccacgcggg cgccggcctc
    11521 gaacagcttg gcggtcttgc agcggatgac gtcgaagtag cgcgactcgt cggtttccgg
    11581 gtcatggcag ttcatgagct gcaggacctc gccttccgcg atgacgttgg tcgcgtccgc
    11641 catgatttcc atgacccgca gctggcccat cccgaccatc atctggaagg cgcgggagta
    11701 gaggaaatcg ccgaccagca cgctcgcctc gttgccgaac acggcgttgg cggtgtcgcg
    11761 gcctcgacgc aggtccgagc cgtccaccac atcgtcgtgc agcagcgtcg cggtgtggat
    11821 gaactccacc accgcggcca cgccgatgtg acggtcgccc tggtagccgc aggcacgggc
    11881 cgcgagcagc accagcagcg gccgcaggcg cttgccgccg ccggccacga tgtgtcgggc
    11941 cacctggttg accagcatga cgccgctgtc gaggctggcg acgatccagc ggtccaggct
    12001 gtccaggtcc cgggcggcga ggtcccggat ctcggagagg gagagccggc cggcggcttc
    12061 ggcagggcgt gcggaggtca gtggtgtcgg catcgtcgct cgcggcggtg cggaagggcg
    12121 tcctgcgccc ggatggcgta ttatcgcgga ttccacgggg ggccgcagcc ccgggaaaac
    12181 ccgcacggca ttgtttgacc cggtcgggat aaatccatag aataaccggc tttctccggg
    12241 tcactccggc ccgggcgcgt gtttatggga gcatccatgt acgccgtgat cgccacaggc
    12301 ggtaagcaat acagggtcag cgaaggcgct gtcctgcgtg tcgagaagct tgacgccgac
    12361 gagggcgcca gcatcgagtt tgaccgggtc ctgctggtcg gcgatggcga cagcgtcagc
    12421 gtcggcaagc cgttcttgga cggcagcaag gtgcaggcga ccgtcatgtc ccagggcaag
    12481 gcccgcaagg tcgaagtcct gaagttccgc cggcgcaaga actaccggcg caccaaggga
    12541 catcgccaga atttcaccca ggtcaagatc accgggatcg tcgccggctg aggcggcgtt
    12601 cgcagaggta acgggcaatg gcacacaaaa aggcaggcgg cagcacgcgc aacggccgcg
    12661 actcacagtc caagcggctc ggcgtcaaga agtacggtgg tgagcaggtg ctggccggca
    12721 atatcatcgt ccgccagcgc ggcaccaggt ttcatcccgg cgtcaacgtc ggctgcggcc
    12781 gggactacac gctgttcgcc aaggcggacg gccgcgtgtt gttcgagacc aaggggcccc
    12841 ataaccgcaa gttcgtgagc atcgtcacgg actgagtccg tcggtcgcgc ttatgctcgc
    12901 aggccccggc atggaccggg gccttcgcgt ttctgggttc cacgagagtc gcggcgcagc
    12961 gggcaaggcg cgaagcacgc aagagatttc cgcatgaaat tcgttgacga ggcgaccatc
    13021 acggtccagg caggtgcggg cggcaacggc tgcatcagct tccggcgcga gaaattcatt
    13081 cccttcggcg gtccggacgg cggtgacggc ggcaacggcg gcagcgtctg gctggtcgcg
    13141 gacgctggcc tgaacacgct ggcggacttc cggcggcagc gccgcttccg tgccgccaac
    13201 ggcgcgccgg gcatgggcag caactgttcc gggcggggcg gcgagtccaa ggagatcatg
    13261 gtgccggtcg gcaccgtcgt gcacgaggag agcaccggcg aaatcctcgg cgacctggtc
    13321 gagccggagc agcggctgct ggtggcgcag ggcggcatcg gcggcaaggg caacacgcac
    13381 ttcaagagca gcgtcaaccg cgccccccgc aagtcgaccc ccggcacgcc gggcgagctg
    13441 cgccacctgc gcctggagct gcggctgctg gccgacgtcg gcctgctcgg cctgcccaat
    13501 gccggcaagt ccaccctgct cagggcggtc accgccgcca ggccgaaagt gggagattac
    13561 cccttcacca cgctgcatcc gaacctcggg gtcgtcgacc tggaggaggg caagaccttt
    13621 gtcatcgccg acattccggg gctgatcgag ggcgccgccg aagggcacgg cctgggcaca
    13681 cggtttctgc ggcacctgca gcgcacccgg ctgctgctgc atgtcgtcga tgcctcccag
    13741 cacgagggcg agccgccgcc ggaacagggg gcgcgcgaca tcatccatga gctcaagtcc
    13801 tacagcgcgg agctggccag ctggccgcgc tggctggtca tcaaca
//
LOCUS       DAOMPG010000042        13477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_1966_length_13477_cov_4.896886, whole genome shotgun sequence.
ACCESSION   DAOMPG010000042 DAOMPG010000000
VERSION     DAOMPG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13477
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_13477_cov_4.896886"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLS34_03710"
     CDS             complement(<1..234)
                     /locus_tag="PLS34_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86609.1"
                     /translation="MSGRAQGDLPRNSPARHRAAWSAAVAAAACFVPALAGCTTHEEP
                     GEQAAAPPPDFVEPRAPRQDPGAPVSNGGTQDLP"
     gene            complement(231..1901)
                     /locus_tag="PLS34_03715"
     CDS             complement(231..1901)
                     /locus_tag="PLS34_03715"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRP86610.1"
                     /translation="MEKIWLKEYPQGVPAEIDVDEFPSLREFLEQSFAKYRSLPAFTN
                     MGHSISFGELDEMSRYLGGWLQKNAGLAKGDRVAVMMPNIIQYAVAVAAILRAGLTVT
                     NINPLYTPRELEHQLKDSGAKAIIIVENFARTLEKVRHNTAVKTVVITAIGDMLPVPK
                     RQLVNFVIRKVKKMVPEYNIPDAVSFHKALKEGKWQTLDRPELDHDDVAFLQYTGGTT
                     GVAKGAILTHRNIIANLLQARAWLNATVTEGQSIVITPLPLYHIFSLTANFFTFTLMG
                     GNNILITNPRDMPGFIKELKKHKFTFITGVNTLFNGLLNTPGFDEVDFSSLKATLGGG
                     MAVQRAVAERWKEVTGKPLIEAYGLTETSPAACINPMTNTEFNGSIGLPISSTEVSIR
                     DEDGRELGIGAVGEICIRGPQVMAGYWNRPDETAKVMLEGGWFRSGDMGRMDDQGYIY
                     IEDRKKDMILVSGFNVYPNEVEGVVVMHPAVLEAAAVGIPDEKSGEIVKLFVVCREGQ
                     SVAEKELIDFCRESLTGYKVPRLVEFRKELPKTNVGKILRRELREGKA"
     gene            complement(1927..2556)
                     /locus_tag="PLS34_03720"
     CDS             complement(1927..2556)
                     /locus_tag="PLS34_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRP86611.1"
                     /translation="MYRILLLFLVLAAPAVSAAPPVVLIVGDSLSAGYGLAIHENWPS
                     VLQQRLREAGYPHRVVNASISGDTTSGGLARLPRALERNSPSIVLIGLGGNDGLRAIP
                     IPEFRRNLANMIQLSQARGAKVLLAGVHIPPNYGPEYTSAFHNVYHELAQEYGTGLIP
                     FILEDVALNPELMMDDGIHPTAAAQPVIVDNVWPALEPLLGATLRQAKQ"
     gene            2555..3238
                     /locus_tag="PLS34_03725"
     CDS             2555..3238
                     /locus_tag="PLS34_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP86612.1"
                     /translation="MQQLAGNQPVLKCSGLTKKVSSPEGELTILDRADLEIRGGESVA
                     ILGPSGAGKSTLLSLLAGLDLPTAGQVWVNGRELTALDEDGRAAVRARQVGFVFQSFH
                     LVPALTALENVMLPLELAGRREARREARAALDDVGLGPRVGHYPRQLSGGERQRVAIA
                     RAFVGSPSVLFADEPTGNLDSRTGAKVADLLFRLNAERGTTLVLVTHDRTLADRCDRQ
                     LEMDSGRIL"
     gene            3235..5706
                     /locus_tag="PLS34_03730"
     CDS             3235..5706
                     /locus_tag="PLS34_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRP86613.1"
                     /translation="MSGLSFGLAQVGRELKSGEVRVLAAALALAVAAMTAVGFFTDRV
                     AQGVQSRSAEVLAADLVLRSNRVIPDERETLAAGLRLATARTASFPSVVLAGEQSALA
                     DIEGVEEGYPLRGSLRVSSRIDAESETVRAVPPPGEAWADPRLLARLDADVGQVVRLG
                     RIELTVTRLLDYRPDQGFSFAELAPTLLVNHADVAASGLVAPGSRVSYRLLLAGPPAA
                     LAAYRDRVELGVGERLRGIEDAQPEIGNAVERAGRFIGLASLVSALIAAVAVAMAARR
                     FAHRRMDTVALMKCVGASRRFVLVAMLSQLLVVGLGAAMIGLGLGWLAQEGLVRLLAD
                     LVGDQLPPPTLAPAIGGVVLAAIVLAGFALVPIIELCRTPPGRVLRRDLLPPAAGPLV
                     LYGAAIGGVLALLAWQVRDATLVGWLALGTAAVVLGLAGGGWLLVQAASRLRGGGAAW
                     RYGLANVSRRGRDSIIQVGAFGIGLMVLLLLTLVRSGLLEQWRATLPPEAPNRFLINI
                     QPHEADGVREFLAQRFGGGELTPLIRARITAINGRPAAEIELATPRAQRFLDREANLS
                     WAREPASDNRVVAGRWWPTDDEAEVSVEVDFARDLGMSLGDRVEFDVAGERLEVKVTS
                     LREVQWDSFNPNFFMVLPPGLMRDYPLTYISSVYVDRERRGDVLELVRSYPSVTVIDI
                     EAAIEQVRAVMDQASLAVQYVFLFALGAGILVMLAVVQSTREERLYESAMLRTLGARR
                     SVVLAGVAVEFAALGMISGVLAAAGATGVAWLLAERVFQLDFTPDPMVWLAGLAAGLF
                     VVGATGVLATRTVVTHPPANVLRGY"
     gene            complement(5789..6694)
                     /locus_tag="PLS34_03735"
     CDS             complement(5789..6694)
                     /locus_tag="PLS34_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRP86614.1"
                     /translation="MKAINIHLSLRALGYLVALAETRHFGKAAERCFVSQPTLSAQIK
                     KMEDQLGVQLVERGQQVRLTDIGARIVERARRVIDEAREIEELARNVQDPLAGELRIG
                     LIPTVGPYLLPHIAGPLHERFPRLRLLLLEHQTHRLIELLRSGELDVAILALPIPMER
                     LVTRVLYTESFQVALPQGHALARRRRLALDDLEGETLLLLEDGHCLRDQALAACRLAK
                     VRETPDFRATSLETLRQMVAAGVGITLLPRLAVEQQDTRAPSMVVRRFQDPEPSRTIA
                     AAWRPGSAREETITHLCDAIEKLMG"
     gene            6794..7339
                     /locus_tag="PLS34_03740"
     CDS             6794..7339
                     /locus_tag="PLS34_03740"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005772008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRP86615.1"
                     /translation="MINIGKKFPAWKLKGVVSNDIDTAFKEFSSDSPDLKGQWKILFF
                     WPKDFTFVCPTEIAAFGKLNGEFKDRDARLFGVSTDSEFVHLAWRNDKEELRDLSFPM
                     LSDIKRELSSELGILDEAEGVAQRATFIVDQDDIVRFVYVTDLNVGRSPEEVLRVLDA
                     LQTDELCPCNWKKGDDTLKAA"
     gene            7350..7904
                     /locus_tag="PLS34_03745"
     CDS             7350..7904
                     /locus_tag="PLS34_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRP86616.1"
                     /translation="MNLEQVKALMPEYARDIKLNLSTVLGGTGTPGLTESQALGTALA
                     CAVAARSNTLAAAVEAVAGDKLDPARRNAARAAAAVMAMNNVYYRFTHFVGDEEYRKL
                     PARLRMNVIGNPGIDKAEFELYSLAVSAINGCEFCIQAHEKVVRDAGIGREGVQSAAR
                     IAAVIAAAAGVLATVSDAEAAQAA"
     gene            complement(7958..9364)
                     /locus_tag="PLS34_03750"
     CDS             complement(7958..9364)
                     /locus_tag="PLS34_03750"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRP86617.1"
                     /translation="MKLAIFWFRRDLRLIDNPGLCAALDAAESVLPVYLWSPGDEGEW
                     APGAASRWWLHQSLTALDKALRAKGSRLLVRRGPAADALARLVADTGATGVFWNRLYE
                     PALVARDAAIKSRLREAGVEAHSSKAALLHEPWELATGKGGPYRVFTPFWKAAQQGPQ
                     PAAAQPAPADLKAPRHWPAGEPPEALGLLPRSRWYDGLAAAWTPGEAGALARLDDWCE
                     ARIGAYAEQRNRPDCDGSSALSPHLHHGEISPRQAWHAATARRAELPARGIEAWLREL
                     GWREFAHHVLYHFPHTPTEPMYEKYAAFPWREDHEAMLAAWQRGRTGIPIVDAGMRQL
                     WATGWMHNRVRMIVASLLVKNIRAPWQSGARWFWDTLVDADLANNTMGWQWTAGSGAD
                     AAPYFRIFNPVTQGEKFDPQGDYVRRWVPELAEVPASGIHQPCEQRTDGYPAPLVDLK
                     ESREEALAALRAIAPEKG"
     gene            complement(9361..10335)
                     /locus_tag="PLS34_03755"
     CDS             complement(9361..10335)
                     /locus_tag="PLS34_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRP86618.1"
                     /translation="MKAVFPDFATVSRDDLDLAPLRATGIDLALYGVTAPAELPARLA
                     AAEILITNKIRIGAAELAAAPRLKLICLSATGVNNVDLEAAGARGIGVCNVTAYCTAS
                     VVQHVYALILALTHHLAAYQRLLQAGAWKTSPQFCLLDFPIRELAGRKLGIVGFGELG
                     RGVARAAPAFGLEVLVSERPGDSAGAARGTKDEPRRPFEQVLAEADILSLHCPLTDDT
                     RGLIDAAALGRMKRDALLINTARGALVDSAALADALRAGRIGGAGIDVLPQEPPVDGD
                     PLLDAAIPNLIITPHVAWAARESRQRAVNEIAANIRSFMAGGRRGRVI"
     gene            10401..11288
                     /locus_tag="PLS34_03760"
     CDS             10401..11288
                     /locus_tag="PLS34_03760"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRP86619.1"
                     /translation="MGTLRGILTMALIVLNLLFWATPLYLVAFLKLLPVPAWRHQCDR
                     ALVSLARNWIGGNDLIFRLTQDIRWDVQGIEGLEPDDWYLVLCNHQSWVDILVLQGVF
                     NRRIPFLKFFLKRQLIWVPVLGFAWWALDFPFMQRHSREYLEKHPEARGQDLAATRRA
                     CEHFSRMPTSVMNFVEGTRFTPAKHAAQGSPYRHLLKPRAGGVAFVMAAMGDILHRIL
                     DVTVVYPGGAPSLWEMCCGKLRKVVVRVAVREIPEWARRGDYQDDPEFRARFQDWLSA
                     LWEQKDRQFGALCAEEGRA"
     gene            11342..11992
                     /locus_tag="PLS34_03765"
     CDS             11342..11992
                     /locus_tag="PLS34_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRP86620.1"
                     /translation="MAARAREFRNELSRRRTVRDYADRAVPRALIEDCLRAAGSAPSG
                     ANLQPWHFVAVADPGLKREIRSAAEAEEREFYAHRAPDDWLAALAPLGTGPEKPFLEV
                     APWLIAIFEQRWGHDARGHKVKHYYPKESVGIATGLLIAALHHAGLATLTHTPSPMKF
                     LNRILGRPPEERPFLLLVVGHPAPDARVPGIARKPLDEIATFLEAAADQGPDDDKN"
     gene            12011..13378
                     /locus_tag="PLS34_03770"
     CDS             12011..13378
                     /locus_tag="PLS34_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRP86621.1"
                     /translation="MEQVTAWLDAVSGFVWGPYCLIPLLLLTGLYLTVLLRGLQFRQL
                     GPALHQALIVRREHGAEGDISHFAALMTAMAATVGTGNIVGVATAIALGGPGALFWMW
                     VTGLVGMATKYSEALLSVKYRVTNPRGEQAGGPMYYLAEGVRWKPLGRTLGWLFALFT
                     ACAAFGIGNMVQSNSVADAIAGAFGVPGWITGAVIAVAVAAVTLGGIRSIGGFTSLFV
                     PAMIVVYMGACTIVLLFNFTAIPAAFALVFTDAFTGSSAAGGFLGATIAQAMRYGVAR
                     GIFSNESGLGSAGIAAAAAKTQEPVRQALVSMTQTFIDTLVVCTFTGIAIIASGAWSS
                     GANGAALTQLAFGSAMPGPIGPAVVAICLAMFAFSTIIGWSHYGSRGIEYILGVRAIL
                     PYRLLFVLAAFLGSVYTLDFVWLLSDVMNGLMALPNLVGLLLLSGVIATETRSYFERQ
                     RSGLP"
BASE COUNT         1908 a         4609 c         4737 g         2223 t
ORIGIN      
        1 cggcaggtcc tgcgtcccgc cgttgctcac gggcgcccca gggtcctgcc gcggcgcgcg
       61 cggctcgacg aagtcgggcg gcggcgcggc ggcctgttcg cccggctcct cgtgtgtcgt
      121 gcagcccgcc agcgccggga cgaaacacgc cgcagcggcc accgccgcgg accaggccgc
      181 gcggtgacgg gcaggggagt tgcgcggcag gtcaccttgc gcgcggccgc tcaagccttg
      241 ccctcacgca gctccctgcg caggatcttg ccgacgttgg tcttgggcag ctccttgcgg
      301 aactccacca gccgcggaac cttgtagccg gtcaggcttt cgcggcagaa gtcgatcagc
      361 tccttctccg ccacgctctg gccctcgcgg cagaccacga acagcttgac gatttcgccg
      421 gacttctcgt ccggaatgcc gaccgcggcc gcctcgagca cggccgggtg catcacgacg
      481 acgccctcga cctcgttcgg gtacacgttg aagcccgaca ccaggatcat gtccttcttg
      541 cggtcctcga tgtagatgta gccctggtcg tccatgcggc ccatgtcgcc actgcggaac
      601 cagccgccct ccagcatcac cttggcggtc tcgtcgggcc ggttccagta gccggccatg
      661 acctggggcc cgcggatgca gatctcgccc accgcgccga tgccgagctc gcggccgtcc
      721 tcgtcacgga tgctgacctc ggtggaggag atcggcagcc cgatggagcc gttgaactct
      781 gtgttggtca tggggttgat gcaggccgcc ggcgatgtct ccgtcaggcc gtaggcctcg
      841 atcagcggct tgccggtaac ctccttccag cgctcggcga ccgcccgctg cacagccatg
      901 ccgccgccga gcgtggcctt caggctgctg aagtcgacct cgtcgaagcc gggggtgttc
      961 agcaggccgt tgaacagcgt gttgacgccg gtgatgaagg tgaacttgtg cttcttcagc
     1021 tccttgatga agccgggcat gtcgcgcggg ttggtgatga ggatgttgtt gccccccatc
     1081 agcgtgaagg tgaagaagtt cgccgtcagc gagaagatgt ggtagagcgg cagcggcgtg
     1141 atgacgatcg actggccttc ggtcacggtg gcgttgagcc aggcgcgcgc ctgcagcagg
     1201 ttggcgatga tgttccggtg cgtgaggatc gcgcccttgg ccacgccggt cgtgccgccg
     1261 gtgtactgca ggaaggccac gtcgtcgtgg tccagttccg gccggtccag ggtctgccac
     1321 ttgccctcct tgagggcctt gtggaagctg acggcatccg ggatgttgta ctccggcacc
     1381 atcttcttga ccttgcggat cacgaaattc accagctgcc gcttgggcac cggcagcatg
     1441 tcgccgatcg cagtgatcac gaccgtcttg acggccgtgt tgtggcgcac cttctccagg
     1501 gtcctggcga aattctcgac gatgatgatc gccttcgcac cggagtcctt gagctggtgc
     1561 tcgagctcgc gcggcgtgta cagcgggttg atgttggtca cggtcaggcc ggcgcgcagg
     1621 atcgcggcca ccgccacggc gtactggatg atgttcggca tcatcacggc gacgcggtcg
     1681 cccttcgcca gcccggcgtt cttctgcagc cagccgccga ggtagcggga catctcgtcc
     1741 agctcgccga agctgatgga gtggcccatg ttcgtgaacg ccggcaggct gcgatatttc
     1801 gcaaacgact gctcgaggaa ttcgcgcagc gaggggaact cgtcgacgtc aatctcggcg
     1861 ggcacgcctt gcgggtattc cttcagccag atcttttcca tggtttcctc gttgactccc
     1921 aggtcatcat tgttttgcct gacgaagcgt cgcgcccagc agcggctcca gcgccggcca
     1981 gacgttgtcc acgatgacgg gctgcgccgc ggcggtcgga tgaatcccgt cgtccatcat
     2041 cagttccggg ttcagcgcca cgtcctcgag gatgaagggg atgagcccgg tgccgtattc
     2101 ttgcgcaagc tcgtggtaga cattgtgaaa ggcgctggtg tattcgggcc cataattggg
     2161 cgggatgtgc acgcccgcca gcaggacctt tgcgcccctt gcctgtgaaa gttgaatcat
     2221 gttggcgagg ttgcgtcgaa actcagggat cgggatggcg cgcagcccgt cgttgccgcc
     2281 gagcccgatg agcacgatcg acggcgaatt gcgctccagg gcccgcggca ggcgggccag
     2341 gccgccgctg gtcgtgtcgc cgctgatgct ggcgttgacg acgcgatgcg ggtagccggc
     2401 ctcccggagg cgctgctgga gcacgctggg ccagttctcg tggatcgcca gaccatagcc
     2461 ggcgctcagg ctgtcgccga caatgagcac cacgggcggc gcggccgaaa cggccggcgc
     2521 ggccaggacc aagaaaagca gaaggattct gtacatgcag caattggccg gaaatcagcc
     2581 ggttctcaag tgttcggggc tgaccaagaa ggttagcagc ccggagggag agcttaccat
     2641 cctggaccgc gccgacctcg agatccgcgg cggcgagtcc gtggcgatcc tgggaccgtc
     2701 gggcgcgggc aagtcgacac tgttgtcgct gctggcaggg ctcgacctgc ccaccgcggg
     2761 gcaggtctgg gtgaacgggc gggagctcac ggccctcgac gaggacgggc gcgcggcggt
     2821 tcgggcacgg caggtgggct tcgtgttcca gtcctttcac ctcgtgccgg cgctcaccgc
     2881 gctggagaac gtgatgctgc cgctggaact ggccggccgc cgggaggcgc ggcgcgaggc
     2941 ccgcgcggcg ctggacgacg tcgggctcgg cccgcgcgtc ggccactatc cccgccagct
     3001 ctcgggtggc gagcggcagc gcgtcgccat cgcccgtgcc ttcgtcggca gcccctcggt
     3061 gctgtttgcc gacgagccca ccggcaacct ggattcccgt accggcgcga aggtggctga
     3121 cctgttgttc cgcctcaacg ccgagcgcgg caccacgctg gtgctggtga cccacgaccg
     3181 cacgctcgcc gaccgctgcg accggcagct cgagatggac agcgggagaa tcctttgagc
     3241 ggcctgtcct tcggcctggc gcaggtcggc cgggagctga agtcgggcga ggtgcgcgtg
     3301 ctggcggcgg cgctggcgct ggccgtggcg gcgatgaccg cggtcgggtt tttcacggat
     3361 cgagtcgccc agggcgtgca gtcgcgcagc gcggaagtgc tggcggcgga cctcgtgctg
     3421 cgttccaacc gggtcatccc ggacgagcgc gagacgctgg ccgccgggct gcgcctcgcc
     3481 acggcccgca ccgcgtcgtt ccccagcgtg gtgctggcgg gtgagcagag cgccttggcc
     3541 gatatcgagg gcgtcgaaga gggctacccg ctacgcggca gcctgcgggt cagcagccgc
     3601 atcgacgccg agtcggagac cgtgcgggcc gtgccgccgc cgggcgaggc ctgggcagac
     3661 ccacgcctgc tggcgcgtct cgacgccgac gtcggccagg tggtgaggct cggcaggatc
     3721 gagctgacgg tcacccggct gctcgattac cggccggacc agggcttcag cttcgcggag
     3781 cttgcaccca cattgctggt caaccacgcg gacgtcgccg cgagcggcct ggtggcgccg
     3841 ggcagcaggg tgtcctaccg gctgttattg gccggtccgc ccgcggcgct ggcggcttac
     3901 cgggaccggg tcgagctcgg cgtgggcgag cgcctgcggg gaatcgagga tgcgcagccg
     3961 gagatcggca atgccgtcga gcgcgccggc cgcttcatcg gcctcgcctc gctggtgagc
     4021 gcgctgatcg cggccgtggc cgtggcgatg gcggcgcgcc gcttcgcgca ccgccgcatg
     4081 gacaccgtgg ccctgatgaa gtgcgtcggc gcctcgcgcc gcttcgtgct ggtggcgatg
     4141 ctctcgcagc tgctggtcgt cggcctggga gcggcaatga tcggcctcgg cctgggctgg
     4201 ctcgcgcagg aggggctggt gcgcctgctg gcggacctgg tcggcgacca gttgccgccg
     4261 ccgaccctcg cgcccgcgat cggcggcgtg gtgctggccg ccatcgtgct ggccggcttt
     4321 gcgctggtgc cgatcatcga gctctgccgc acgccgcccg gccgggtgct gcggcgggac
     4381 ctgctgccac cggccgccgg gccgctggtg ctgtacggcg ccgcgatcgg cggcgtgttg
     4441 gccctgctgg cctggcaggt gcgcgacgcc acactggtcg gctggctggc gctcggcacg
     4501 gctgcggtgg tgctggggct ggccggcggc ggctggctgc tggtgcaggc ggcttcgcgt
     4561 ctgcgcggcg gcggcgccgc ctggcgctac ggcctcgcca acgtcagccg ccggggtcgg
     4621 gacagcatca tccaggtcgg ggcgttcggt atcgggctga tggtgctgct gctcctgacc
     4681 ctggtgcgct cgggcctgct cgagcagtgg cgcgccacgc tgccgccgga ggcgcccaac
     4741 cgtttcctga tcaatatcca gccgcacgag gcggacggcg tgcgcgagtt cctggcgcag
     4801 cgcttcggcg gcggcgagct gacgccgctg atccgcgcac ggatcacggc gatcaacggt
     4861 cgtccggccg cggagatcga gcttgcgacg ccgcgtgcgc agcgcttcct ggatcgggaa
     4921 gcgaatctca gctgggcccg cgaaccggcg tccgacaacc gcgtggtggc cggccgctgg
     4981 tggccgaccg acgacgaggc cgaagtctcc gtggaagtcg atttcgcccg tgacctgggc
     5041 atgagcctgg gcgaccgggt cgagttcgac gtcgccggtg agcggctcga ggtcaaggta
     5101 accagcctgc gcgaagtgca gtgggattcc tttaatccga acttcttcat ggtcttgccg
     5161 ccgggactga tgcgcgacta tccactgacc tacatttcca gcgtgtacgt ggaccgcgag
     5221 cggcgcggcg acgtgctgga gctggtgcgc agctacccga gcgtcacggt gatcgacatc
     5281 gaggcagcca tcgagcaggt gcgggccgtg atggaccagg cctcgctggc ggtgcagtac
     5341 gtgttcctgt tcgcactcgg cgcgggcatc ctggtgatgc tggcggtggt gcagtcgacg
     5401 cgcgaggagc gcctctacga gagcgcgatg ttgcgcacgc tgggtgcgcg ccgcagcgtg
     5461 gtgctggccg gtgtggcggt ggaattcgcg gcgctgggca tgatctccgg cgtgctggcc
     5521 gcggcgggcg ccaccggcgt cgcctggttg ctggccgagc gggtgttcca gctcgacttc
     5581 acgcccgacc cgatggtctg gctggcgggc ctcgcggccg ggctgttcgt cgtcggcgcc
     5641 accggtgtgc tggcgacccg caccgtcgtc acccatccgc ccgcgaacgt gctgcggggt
     5701 tactgaaaat ggagaaaccg gggtcagaca ctcatttcca aaagaaatgg gtgtctgacc
     5761 ccggtttctc cggtttctcc ccagttttct agcccatgag tttctcgatt gcgtcacaga
     5821 ggtgagtgat ggtctcctcg cgggcgctgc ccgggcgcca ggcggcggca atggtgcgcg
     5881 agggctccgg gtcctggaaa cgccgcacga ccatggacgg ggcacgcgtg tcctgctgct
     5941 cgacggcgag cctcggcaac agcgtgatgc cgacgcccgc ggccaccatc tggcgcagcg
     6001 tctcgaggct ggtggcgcgg aagtccggcg tttcgcgcac cttcgccagc cggcacgccg
     6061 ccagggcctg gtcgcgcagg cagtggccgt cctccagcag cagcagcgtc tcgccctcga
     6121 ggtcgtcgag agccaggcgc cggcgcctgg ccagcgcgtg gccctgcggc agcgcgacct
     6181 ggaaactctc ggtgtagagc acccgcgtca ccagccgctc cattggaatc ggcagggcca
     6241 ggatcgccac gtcgagctcg ccgctcctga gcagctcgat caggcgatgg gtctggtgct
     6301 ccagcaacag cagcctgagc cgcgggaatc gctcgtgcag cggaccggcg atgtgcggca
     6361 gcaggtaggg cccgacggtc ggaatcagcc cgatgcgcaa ctcgcctgcc agcgggtcct
     6421 ggacgttgcg cgccagctcc tcgatctcgc gcgcctcgtc gatgacccgc cgcgcgcgtt
     6481 ccacgatgcg cgccccgatg tcggtcagcc gcacctgctg gccgcgctcg accagctgta
     6541 cgccgagctg gtcctccatt ttcttgatct gcgcgctgag cgtcggctgg ctgacgaagc
     6601 agcgctccgc cgccttgccg aagtggcggg tctcggccag cgcgacgagg tagccgaggg
     6661 ccctcaggct gagatggata ttgatagcct tcatctatcg aaacgatagg ttcaattcat
     6721 tggataaatc aaggccctgt tggcatgatg cgaagcaaca ttttgcccag cacctgccgc
     6781 aaggagtacg gacatgatca acatcggcaa gaaattcccc gcctggaaac tcaagggcgt
     6841 cgtcagcaac gacatcgaca ccgcgttcaa ggagttctcc agcgacagcc cggacctcaa
     6901 gggccagtgg aagatcctgt tcttctggcc gaaggacttc accttcgtgt gccccaccga
     6961 gattgccgcc ttcggcaagc tcaacggtga attcaaggac cgcgacgccc gcctgttcgg
     7021 cgtctccacg gattccgagt tcgtgcacct cgcgtggcgc aacgataagg aggagcttcg
     7081 cgacctgtcc ttcccgatgc tgtccgacat caagcgtgaa ctgagcagcg agctcggcat
     7141 cctcgacgag gccgagggcg tcgcccagcg cgccacgttc atcgtcgacc aggacgacat
     7201 cgtgcgcttc gtctacgtca ccgacctgaa cgtcggccgc agccccgagg aagtgctgcg
     7261 cgtgctcgac gcgctgcaga ccgacgagct ctgcccctgc aactggaaga agggcgacga
     7321 caccctgaag gcggcgtaag ggggcaatga tgaatctcga acaggtcaag gcgctcatgc
     7381 ccgagtatgc gcgtgacatc aagctgaacc tttccaccgt gctggggggc accggcacgc
     7441 cggggctcac cgagagccag gcgctcggca cggcgctcgc ctgcgccgtg gcggcccgga
     7501 gcaatacgct ggcggccgcg gtcgaggccg tggcgggcga caagctcgac ccggcccgcc
     7561 gcaacgcggc ccgggcggct gccgcagtga tggcgatgaa caacgtctat taccgcttca
     7621 cccacttcgt gggcgacgag gagtatcgca agctgccggc ccggttgcgg atgaacgtca
     7681 tcggcaaccc gggcatcgac aaggcggagt tcgagctcta ttcgctcgcc gtctcggcga
     7741 tcaacggctg cgaattctgc atccaggcgc acgagaaggt cgtgcgcgac gccggcatcg
     7801 gccgcgaggg cgtgcaaagc gccgcgcgca tcgccgcggt gatcgccgcg gcggccggcg
     7861 tgctggccac ggtgtccgat gcggaggccg cacaagcggc ctgagggcgg ggcgccgccc
     7921 gcgagggtgg cgcccttttt tcactcgcgg ggggccttca gcccttttcc ggggcgatgg
     7981 cccgcagggc cgccagcgct tcctcgcgtg attccttcag gtccacgagc ggcgccggat
     8041 agccatcggt ccgctgctcg caaggctggt gaatcccgct tgccgggacc tcggccagct
     8101 cgggcaccca gcgccgcacg taatcgccct gcgggtcgaa tttctcgccc tgggtcaccg
     8161 ggttgaaaat ccggaaatag ggcgccgcgt ccgcccccga gccggccgtc cactgccagc
     8221 ccatggtgtt gttggcgaga tcggcgtcca ccagcgtgtc ccagaaccag cgcgcgccgg
     8281 actgccaggg cgcacgaatg ttctttacca gcagcgacgc gacgatcatg cgcacgcggt
     8341 tgtgcatcca gccggtcgcc cacagctggc gcatgccggc atccacgatc ggaatgccgg
     8401 tgcgcccgcg ctgccaggcc gccagcatgg cctcgtgatc ctcacgccag gggaaggcgg
     8461 cgtatttctc gtacatcggc tccgtcggcg tgtgcgggaa gtgatagagc acgtggtgcg
     8521 cgaactcccg ccagcccagc tcgcgcagcc aggcctcgat gccgcgcgcc ggcagctcgg
     8581 cgcgtcgcgc ggtcgccgcg tgccaggctt gccggggcga gatctcgccg tggtgcaggt
     8641 gtggcgagag cgccgagctg ccgtcgcagt cgggccggtt gcgctgctcg gcgtatgcac
     8701 cgatgcgcgc ctcgcaccag tcgtcgagcc gtgccagcgc cccggcctcg cccggcgtcc
     8761 aggccgccgc caggccgtcg taccagcggc tgcggggcaa cagcccgagc gcctccggcg
     8821 gctcgccggc cggccagtgc cgcggcgcct tcaggtcggc cggggcaggc tgcgccgctg
     8881 ccggctgcgg cccttgctgt gcggccttcc agaagggcgt gaacacgcgg tatggcccgc
     8941 ccttgccggt cgccagctcc cagggctcgt gcagcagcgc ggccttgctg ctgtgcgcct
     9001 cgaccccggc ctcgcgcagc cgcgacttga tcgcggcatc gcgcgccacc agcgccggct
     9061 cgtagagacg gttccagaac acgcccgtag cgcccgtgtc cgcgaccagc cgcgccagcg
     9121 cgtcggctgc cggtccgcgg cggacgagca gccggctgcc cttcgcacgc agggccttgt
     9181 ccagtgccgt gaggctctgg tgcagccacc agcggctggc cgcccccggc gcccactcgc
     9241 cctcgtcccc gggcgaccac aggtagaccg gcagcaccga ttccgcggcg tcgagcgcgg
     9301 cgcacagccc ggggttgtca atcagccgga ggtcccgacg aaaccagaag atcgcgagtt
     9361 tcaaatcacc cggccgcggc ggccgcctgc catgaatgag cgaatgttgg cagcaatttc
     9421 gttcaccgcc cgctggcggg attcacgcgc ggcccaggcg acgtgcggcg tgatgatgag
     9481 gttggggatg gcggcgtcga gcagcgggtc gccatccact ggcggttcct gcggcaagac
     9541 gtcgatgccg gcgccgccga tgcgccccgc gcgcagggcg tccgccagcg ccgccgagtc
     9601 caccagggcg ccgcgcgcgg tgttgatcaa gagcgcgtca cgcttcatgc ggccgagcgc
     9661 cgcggcatcg atcaggccgc gggtgtcgtc ggtcagcggg cagtgcagcg acaggatgtc
     9721 cgcctcggcc agcacctgct cgaagggccg ccgtggctcg tccttcgtcc cgcgggccgc
     9781 gccggccgag tcgcccggcc gctcgctcac cagcacctcc agcccgaagg caggtgcggc
     9841 ccgcgccaca ccccggccca gttcgccgaa gccgacgatg ccgagcttgc gcccggccag
     9901 ttcgcgaatg ggaaagtcga gcaggcagaa ctgcgggctg gtcttccacg cgcccgcctg
     9961 cagcagccgc tggtatgcgg cgaggtggtg ggtgagggcg agaatcagcg catagacgtg
    10021 ttgcacgacc gacgcggtgc agtaggccgt gacgttgcag acaccgatgc cgcgtgcgcc
    10081 ggcagcctcc aggtccacgt tgttcacgcc ggtcgcggac aggcagatca gcttcaggcg
    10141 cggcgcggca gcgagctctg cggcgccgat gcggatcttg ttggtgatca ggatctcggc
    10201 cgccgcgagc cgcgccggca attccgccgg cgcggtgacg ccgtacagcg ccaggtcgat
    10261 gccggtggcc cgcaggggcg cgagatcgag gtcgtcgcga ctgaccgtgg caaagtccgg
    10321 aaacaccgct ttcatggccg gcaagactac acgtccggcg ggcgacccgg ccagcgcgga
    10381 tggggctaca atgcgcggcc atgggtaccc tgcgcggcat cctcaccatg gcgctgatcg
    10441 tcctgaacct gctgttctgg gcgaccccgc tgtacctcgt cgcgttcctg aagctgctgc
    10501 ccgtcccggc gtggcggcac cagtgcgatc gtgcgctggt cagcctggcg aggaactgga
    10561 tcggcggcaa cgacctcatt tttcgcctca cccaggacat ccgctgggac gtgcagggca
    10621 ttgagggact cgagccggac gactggtacc tggtgctgtg caaccaccag agctgggtcg
    10681 acatactcgt gctgcagggc gtgttcaacc gccggattcc cttcctcaag ttcttcctca
    10741 agcgccagct gatctgggtg ccggtgctcg gcttcgcctg gtgggcgctg gacttcccgt
    10801 tcatgcagcg gcactcgcgc gagtacctgg aaaagcaccc cgaggcgcgc gggcaggatc
    10861 ttgccgcgac ccggcgggcc tgcgagcact tcagccgcat gccgacctcg gtgatgaact
    10921 tcgtggaggg cacgcgcttt acgccggcaa aacacgccgc gcagggctcc ccctaccgcc
    10981 acctgctcaa gccgcgcgcc ggcggcgtcg ccttcgtcat ggcggccatg ggcgacatcc
    11041 tgcatcgcat cctcgacgtc acggtggtgt atccgggcgg cgcgccgagc ctgtgggaaa
    11101 tgtgctgcgg caagctccgc aaggtcgtcg tgcgggtcgc cgtacgggag attccggagt
    11161 gggcgcggcg tggcgactac caggacgacc cggagttccg ggcgcgcttc caggactggc
    11221 tgagcgcgct ctgggaacag aaggaccgcc agttcggggc gctgtgcgcc gaggaaggcc
    11281 gcgcgtgagg tccgccggat tgcagccgct ggcgggctac ctggagcttc cggtcgagga
    11341 aatggcagcg cgggcgcgcg agttccgcaa cgagctctcc cggcgccgca ccgtgcgtga
    11401 ctacgccgac cgcgccgtgc cgcgtgcgct gatcgaggac tgcctgcgcg cggcgggcag
    11461 tgcgccctcc ggcgccaacc tgcagccgtg gcacttcgtc gcggttgcgg atcccggctt
    11521 gaagcgggag atccgcagcg cggccgaggc cgaggaacgc gagttctacg cgcaccgcgc
    11581 gcccgacgac tggctggccg cgctggcacc gctgggcacc ggcccggaaa agccgtttct
    11641 cgaagtggcg ccctggctga tcgcgatctt cgagcaacgc tgggggcacg acgcgcgcgg
    11701 ccacaaggtc aagcattact atccgaagga atccgtcggc atcgccaccg gcctgctgat
    11761 cgcggccctg catcatgccg gcctggcgac gctgacgcac acgccgagcc cgatgaagtt
    11821 tctcaaccgc attctcggcc ggccgcccga ggaacggccg ttcctgttgc tggtggtcgg
    11881 ccatcccgcg ccggacgcgc gtgtgcccgg catcgcccgc aagcccctcg acgagatcgc
    11941 caccttcctc gaggcggcgg ccgaccaggg ccccgacgac gacaagaact gacctgggag
    12001 cgcaagcacc gtggaacaag tgaccgcctg gctcgatgcc gtatcgggat tcgtctgggg
    12061 gccgtactgc ctcattccgc tgctgctgct caccggcctg tacctcaccg tcctgctgcg
    12121 cggcctgcag ttccgccagc tcggcccggc cttgcaccag gcgttgatcg tgcgccgcga
    12181 gcacggcgcc gagggcgaca tctcgcattt cgccgcgctg atgacggcca tggcggccac
    12241 ggtcggcacc ggcaacatcg tcggggtggc gaccgccatt gcgctcggcg gtcccggcgc
    12301 gctgttctgg atgtgggtca ccggcctggt gggcatggcg accaagtact cggaggcgct
    12361 gctgtcggtg aagtaccgcg tcaccaaccc gcgcggcgag caggccggtg gcccgatgta
    12421 ctacctggcg gagggcgttc gctggaagcc gctggggcgc acgctgggct ggctgttcgc
    12481 gttgttcacc gcctgcgccg ccttcggcat cggcaacatg gtgcagtcca actcggtggc
    12541 cgacgccatc gccggcgcct tcggcgtgcc cggctggatc accggcgcgg tcatcgccgt
    12601 cgccgtcgcg gccgtgacgc tcggcggcat ccgctcgatc ggcggcttca ccagcctgtt
    12661 cgtgccggcg atgatcgtgg tctacatggg cgcctgcact atcgtgctgt tgttcaactt
    12721 cacggccatc ccggcggcct tcgcgctggt gttcacggat gccttcaccg gcagttcggc
    12781 cgccggcggt ttcctcggcg ccaccatcgc ccaggcgatg cgctacggcg tggcgcgcgg
    12841 cattttctcg aacgagtccg gtctcggttc cgccggcatc gcggcggcgg cggccaagac
    12901 ccaggagccg gtgcggcagg cgctggtgtc catgacccag accttcatcg acacgctggt
    12961 ggtgtgcact ttcacgggga ttgccatcat cgccagcggc gcgtggagtt ccggcgccaa
    13021 cggcgcggcc ctgacccagc tggccttcgg ctcggcgatg cccggcccca tcggcccggc
    13081 ggtcgtcgcg atctgcctgg cgatgttcgc cttctccacc atcatcggct ggagccatta
    13141 cggatcgcgc ggcatcgagt acatcctcgg tgtccgcgcg atcctgccct accgcctgct
    13201 gttcgtgctg gctgccttcc ttggctccgt ctacacgctc gatttcgtct ggctgctgtc
    13261 ggacgtgatg aacggcctga tggcgttgcc gaacctggtc ggcctgctgc tgctgtcggg
    13321 cgtcatcgcg accgagaccc gcagctactt cgagcgtcag cggtccggct tgccctagcc
    13381 cttgcgcccg cccttggcgc gggcgcggcg gatgccgccg aacaccagcg cgaaagccgc
    13441 caccagcagg ggcaccagaa ggatattcag gaaggcc
//
LOCUS       DAOMPG010000043        13230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2022_length_13230_cov_3.832638, whole genome shotgun sequence.
ACCESSION   DAOMPG010000043 DAOMPG010000000
VERSION     DAOMPG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13230
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_13230_cov_3.832638"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLS34_03775"
     CDS             complement(<1..533)
                     /locus_tag="PLS34_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRP86622.1"
                     /translation="MTIRAAVLFDLDGTLADTAPDLAAALNAMLRADGRAPLPYEQVR
                     PWVSWGALRLIRLGYGEALDAATTAELRGRFLASYEACLCRESRLFPGIPEALAALES
                     AAVPWGIVTNKPGWLTEPLLGALGLGDRAGTIISGDTLPFAKPHPMPLLHAARELGLP
                     AARCVYVGDNVRDIEAGR"
     gene            complement(530..1249)
                     /gene="ubiG"
                     /locus_tag="PLS34_03780"
     CDS             complement(530..1249)
                     /gene="ubiG"
                     /locus_tag="PLS34_03780"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRP86623.1"
                     /translation="MTETRNANVDRAEIARFEALADRWWDTEGDFKPLHDINPARLEF
                     IEQRAGLAGRRVLDIGCGGGILAESMAARGARVTAIDMAEAPLAVARIHVRESGLDVE
                     YLKSTAEEHADARPGAYDTVTCLEMLEHVPDPGQVIAACHRLVKPGGHVFFSTINRNP
                     KAWVMAIFGAEYVLRLLPRGTHEYAKLIRPSELADWIRAAGLELTGFTGMHYNPFTRD
                     CTLGGNVHVNYLAHARKREAG"
     gene            complement(1242..2570)
                     /locus_tag="PLS34_03785"
     CDS             complement(1242..2570)
                     /locus_tag="PLS34_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HRP86624.1"
                     /translation="MEKVDTLIKARWVAPVVPAGGVREYHAVGFRQGRIVAVLPAAEA
                     AARFDAAEIVERPLHLLMPGLVNAHTHASMSLLRGLADDLPLMAWLQDHIWPAEQRWM
                     SAEFVEHGAELAMAEMLLGGTTTFNDMYFFPEVVARAAARIGMRACVGMILIKFPTVW
                     AGDSAEYLRKGLALRDEYKGHPLISTAFAPHAPYTVEDEYLAKIRRLADELEAPVHTH
                     VHETADEVLGSVVDCGERPLARLDRLGLVSPMLVAVHMTQLEGAEIERVAAAGASVVH
                     CPESNLKLASGFCPVEKLLAAGVNVCLGTDGAASNNDLDMLGEMRSAALLAKGVAGRA
                     DAVTAEQALAMATINGARALGLADEIGSIEAGKWADLCCVDLGRPSTWPVYDPVAQLV
                     YAAGRDQVEDVWVAGRRMVADGRLARMDDAAIMARADEWRKRIAGADRHD"
     gene            2688..3728
                     /gene="mtnA"
                     /locus_tag="PLS34_03790"
     CDS             2688..3728
                     /gene="mtnA"
                     /locus_tag="PLS34_03790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRP86625.1"
                     /translation="MEPLDPTGIHAVTWRDGRLDLLDQRRLPADVRRVVCEDVDAAIH
                     SIRDMVVRGAPAIGITAGYIAVLAARNRIAAGEADWLAGFRQDMVRLRKARPTAVNLA
                     WAVDRMLAAAASAGSDPVAGLLAEARRIHAEDIEMNLAMAARGADLIETRGSVITHCN
                     AGALATGGVGTALGVIREAWRRGLVTRVWVDETRPWLQGARLTAWELGRDGIPATVIT
                     DSAAAQVMRSESPSWAIVGADRVAANGDVVNKIGTYGLAVAARHHGVRFMVVAPSSTL
                     DAATASGDEVEIEQRDPAEIWAAAGGVPAGADARNAAFDVTPAALIDALVTERGVAAP
                     PLGPALEALLRG"
     gene            3862..6447
                     /gene="gyrA"
                     /locus_tag="PLS34_03795"
     CDS             3862..6447
                     /gene="gyrA"
                     /locus_tag="PLS34_03795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRP86626.1"
                     /translation="MSEVAKEILQVNLEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMRELGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSMRYVL
                     VDGQGNFGSVDGDSAAAMRYTEVRMAKLAHELLADIDKETVDFVANYDESEHEPSVLP
                     TRVPNLLVNGSAGIAVGMATNIPPHNLREIVNACIALIDDPELSIDGLMQHVPGPDFP
                     TAGVINGASGIVEAYRTGRGRVYIRARAHFEPFRDGSREAIVITELPFQVNKARLVEK
                     IAELVREKKLEGISELRDESDKDGLRVVIELKRGENGEVVLNNLFKHTPLQTVFGINM
                     VALVDGQPRLLNLKEILEAFIRHRREVVTRRTIFELRKARDRAHVLEGLAVALANIDE
                     VIALIKASPSSAEARVALTARGWAPGVVTGLLERAGAEATRPAGLAAEFGLGAGGYRL
                     SEAQAQAILDLRLHRLTGLEQEKIIKEYEEIIEKIKDYSEILESPDRLSAVIREELIA
                     VRDDYGDDRRTEILRNHLDLSMEDLISEEDVIVTLSHGGYAKAQPVTDYQAQRRGGRG
                     RAATRVKEEDFVDKLFIANTHDWLLCFSSRGKVYWLKVYELPIAGRGSRGKPIVNLLP
                     LGEGERINAVLPIREFIEGPYVFMATSRGTVKKTPLVAFSRPRRDGIIAIDLRDDDYL
                     IDVGITDGRQQIMLCASNGKAIRFKEADVRPMGRTAGGVRGLKLADEHQVIALIIADE
                     GHLLIATEHGYGKLTPVEDFPVHGRGGQGVIAIQTNERNGLMVGALLVQPDDQVMMIS
                     STGVLVRTTVEGIPALTRNTQGVRLIRLDEGGRLVGMGRVAADQADDLDDDGGDAGDG
                     ENGNE"
     gene            6472..7551
                     /gene="serC"
                     /locus_tag="PLS34_03800"
     CDS             6472..7551
                     /gene="serC"
                     /locus_tag="PLS34_03800"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRP86627.1"
                     /translation="MDRVFNFSAGPAAIPLPVLERARDELTDWRGCGMSVMELSHRGK
                     EFMSIAERAEADLRRLLDIPAGYRVLFLQGGASAQFGAVPFNLAGPDSVADYVVTGSW
                     SKKALGEGRRFLEARVAADSADNRHTAIPDPASWRLSPDAAYLHYTPNETIGGVEFHF
                     VPAASAPLVADMSSTILSRPLDVSRFGLIYAGAQKNIGPAGLAIVIVREDLLGRAREG
                     TPVMMDYGQQAENGSMLNTPPTWGWYVAGLVFEWLLEQGGLAEMERRNRHKAETLYAA
                     IDGSDFYANPVAKHCRSWMNVPFTLADPALDARFLEQAKAAGLLTLKGHRSVGGMRAS
                     LYNAMPQEGVDALVAFMREFERSHG"
     gene            7621..8802
                     /locus_tag="PLS34_03805"
     CDS             7621..8802
                     /locus_tag="PLS34_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRP86628.1"
                     /translation="MTFKILTLNNIALAGLERLPRSLYEVASEIGHPDAVMVRSADMH
                     KTEVPASVKAIGRAGAGVNNIPVEEMSRRGVPVFNAPGANANAVKELVLGGMLLAARN
                     LCESWDYVRTLDGSEDELHRAVESGKKRFVGFELPGRTLGVIGLGAIGVEVANAALSL
                     GMQVIGFDPKMTVQRAWQLNSGVRQAMSLDDLFSRAQVVSVHVPLAEGTRALVNADRL
                     ALMKPGGVLLNFSRGAIVDDAAVLASLDEGRLARYVTDFPSMMNKGHSKVIALPHLGA
                     STREAEENCAIMVAENLRDFLENGNIRHSVNFPEAILPRIERNTRIAIANANVPNMVG
                     QISTSLAEAGLNISDLLNKSRGDVAYTLVDVDGKVPGEVVEKLAAIEGVLSLRVIPDA
                     A"
     gene            8810..9919
                     /gene="pheA"
                     /locus_tag="PLS34_03810"
     CDS             8810..9919
                     /gene="pheA"
                     /locus_tag="PLS34_03810"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRP86629.1"
                     /translation="MSDAPANDMLEDIRRRIDAIDEQLQALISQRAELARAVGATKFP
                     EPAGLSNAPRDYYRPEREAEILRKAVARNSGPLKDEEIVRLFREIMSACLAQQEPMKV
                     AFLGPEGTFSQAAVLKHFGHSVRTLAVPSIEEVFHDVEAGIADFGVVPVENSTEGTVS
                     TTLDMLVVTPLRISGEIELRIRQHLLGRMAGLDEVRRVYSHQQSLAQCRAWLDQRLPG
                     VEQVAVASNAEAARLAAREAGAAAIAGDTAAEVYGLAVLMANIEDRPDNTTRFVVIGR
                     DMPGPSGRDKTSLLLSSRHAAGALQELLHPLSKHNVSMTRIESRPSRRGKWDYVFFVD
                     VEGHREDAHVAAALRELETAAAWCRVLGSYPTAIL"
     gene            9947..11068
                     /gene="hisC"
                     /locus_tag="PLS34_03815"
     CDS             9947..11068
                     /gene="hisC"
                     /locus_tag="PLS34_03815"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRP86630.1"
                     /translation="MTDIYLDLAAPGIRGLKPYQPGKPIAELQREYGVSDIVKLASNE
                     NPLGPAPRALTAIAAAAVELARYPDANGFELKQALADRHAVGPGQVTLGNGSNDVLVL
                     LAESFLGPGLEAVYSRHAFAVYALAVQATGAVHRVAEALPAGGPQPLGHDLDAMAELV
                     GPRTRMVFVANPNNPTGTWVDAAALERFVAGLPRHVVAVVDEAYFEYVQAPDYPDATR
                     WLDRFPNLVVTRTFSKAYGLAGLRIGYSVSSAAIADILNRIRQPFNTNSLAQAAALAA
                     LDDTAHLARSVELNTAEMARVATACRSLGLGENPSVGNFLLVDMGGPAQPLFDALLRE
                     GVIVRPVANYGLPQHLRISIGLPAENDRLIAALEKVTRQ"
     gene            11065..12384
                     /gene="aroA"
                     /locus_tag="PLS34_03820"
     CDS             11065..12384
                     /gene="aroA"
                     /locus_tag="PLS34_03820"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRP86631.1"
                     /translation="MNAPAWRVSPGGTLAGQLQIPGDKSVSHRVMLLGAIGSAPLEAH
                     GFLRSEDCLATRRAVEALGARVEDLANGGVRVAPAAALRSPAEPLDFGNSGTGIRLAT
                     GLLAGHRVDAVLTGDASLCRRPMERVAEPLRRMGALLATSHGCPPIRVSAAGPLRPLD
                     YTMPVASAQVKSALLLAGLSAEGRTVIRQPAVSRDHTERLLAALGCPVESGEWGAAVT
                     GPAQPRGGRVTVPGDFSSAAFLIVGGLLAAGDAPLELLGVGMNPTRTGLLDILTMMGG
                     RVEITRLREECGEPVADLRVWRSELQGVDVPPALVPLAIDEFPALFVAAALARGTTRV
                     RGAEELRVKESDRIAVMARGLAAVGVQVAERADGLLIEGGRLAGGTVDSGGDHRVAMS
                     FAVAAARAEAAIEILDVANVATSFPGFAAAAASLGLQIETLRAGAAA"
     gene            12381..13055
                     /gene="cmk"
                     /locus_tag="PLS34_03825"
     CDS             12381..13055
                     /gene="cmk"
                     /locus_tag="PLS34_03825"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRP86632.1"
                     /translation="MSAAPVLAVDGPGGAGKGTLCRRVAEQLGWQLLDSGALYRLVAV
                     AAADAGTALDDEPGLAALARDLPVRFAGERVLLGGRDVSAEIRSETCGERASRVAALP
                     AVRTALVGLQRSFRRPPGLVADGRDMGTVIFPDAELKVFLTASAEERAMRRYKQLKEK
                     GFDVNLRDLSAEVAARDRRDAERAVAPLRPAPDAVVLDSTELSIDQVVEAVLDELQRR
                     GLGPTG"
BASE COUNT         2056 a         4465 c         4714 g         1995 t
ORIGIN      
        1 cgtccggcct cgatgtcgcg tacgttgtcg ccgacgtaca cgcaacgggc ggccggcagc
       61 cccagttccc gcgcggcgtg cagcaggggc atgggatggg gcttggcgaa gggcagggta
      121 tcgccgctga tgatggtgcc ggcgcgatcg cccagaccca gcgcgccgag caaaggctcg
      181 gtgagccagc ccggcttgtt ggtcacgatg ccccagggca cggcggcgga ctcgagtgcg
      241 gcgagtgcct cggggatgcc gggaaacagg cgcgactccc ggcacaggca ggcttcgtaa
      301 ctggccagga agcggccccg cagctcggcg gtagtcgccg cgtccagtgc ctccccgtag
      361 cccagccgga tcagccgcag cgcgccccag gacacccagg gacgcacctg ttcatacggc
      421 agcggtgccc gcccgtcggc gcgcagcatg gcgttgagcg cggcggccag gtccggcgcg
      481 gtgtcggcaa gcgtgccgtc gagatcgaac aggaccgcgg cgcgaatggt cacccggcct
      541 cccgcttgcg cgcatgggcg aggtaattga cgtgcacatt gccgccgagc gtgcagtcgc
      601 gtgtgaacgg gttgtagtgc atgccggtga agcccgtaag ctccagcccg gccgcgcgaa
      661 tccagtcggc cagttcggag ggccggatca gcttggcata ttcgtgcgtg ccgcgtggca
      721 gcagccggag cacgtactct gcgccgaaaa ttgccatcac ccaggccttg ggattgcgat
      781 tgatggtcga aaagaacacg tggccacccg gcttcaccag gcggtgacat gcggcgatca
      841 cctggccggg atcggggacg tgctccagca tctccaggca agtcaccgtg tcataggcgc
      901 cgggccgtgc gtcggcgtgc tcctcggcgg tggacttgag gtattcgacg tcgaggcccg
      961 actcacgtac gtggatccgg gccaccgcca gcggcgcctc ggccatgtcg atggcggtga
     1021 cccgcgcgcc gcgcgcggcc atcgactcgg cgaggatgcc gccgccgcag ccgatgtcga
     1081 gcacgcgccg gcccgccagt ccggcccgct gctcgatgaa ctccagccgg gccgggttga
     1141 tgtcgtgcag cggcttgaaa tcgccctcgg tgtcccacca gcgatcggcc agcgcctcga
     1201 aacgcgcgat ctccgcccgg tcgacgttgg cgttacgggt ctcagtcatg tcggtcggct
     1261 cctgcaatcc gctttcgcca ttcgtcggcg cgcgccatga tggcggcgtc gtccatgcgc
     1321 gccaggcggc cgtccgccac catgcgacgc cccgccaccc agacgtcctc gacctggtcg
     1381 cggccggcgg cgtacaccag ctgcgcgacg gggtcgtaga ccggccaggt cgagggacgc
     1441 ccgagatcca cgcagcacag gtcggcccac ttgcccgcct cgatcgagcc gatctcgtcc
     1501 gccagcccga gcgcacgcgc gccgttgatg gtggccatgg cgagcgcctg ttcggcggtc
     1561 acggcatcgg cccggcccgc gacgcccttg gccagcagcg ccgcgctgcg catttcgccg
     1621 agcatgtcga gatcgttgtt gctggccgcg ccgtcagtgc cgaggcatac gttgacgccg
     1681 gcggcgagca gcttctccac cggacagaat ccgctggcga gcttcaggtt ggattcaggg
     1741 cagtgcacca cgctggcgcc cgcggccgcc acgcgctcga tctccgcgcc ctccagctgg
     1801 gtcatgtgga cggccaccag catgggtgaa accaagccga ggcggtcgag ccgcgccagc
     1861 ggccgctcgc cgcagtccac gacgctgccg agcacttcgt cggcagtctc gtggacgtgg
     1921 gtgtgaacgg gtgcctcgag ctcgtccgcc aggcggcgga tcttggccag gtactcgtcc
     1981 tcgaccgtgt agggcgcatg gggcgcgaag gcggtcgaga tcagcggatg gcccttgtac
     2041 tcgtcgcgca gcgccaggcc cttgcgcagg tactcggccg agtcgccggc ccacacggtc
     2101 ggaaacttga tgaggatcat gccgacgcag gcgcgcatgc cgatgcgcgc cgccgcgcgc
     2161 gccacgacct cggggaagaa atacatgtcg ttgaatgtcg tggtgccgcc gagcaacatc
     2221 tcggccatcg cgagctcggc cccgtgctcc acgaactcgg cgctcatcca gcgctgctcg
     2281 gcgggccaga tatggtcctg cagccacgcc atgagcggca ggtcgtcagc caggccgcgc
     2341 agcagcgaca tcgatgcatg agtgtgggca ttgaccagcc cgggcatcag caggtgcagc
     2401 ggccgctcga cgatctccgc cgcatcgaaa cgcgccgccg cctcggccgc cggcagcacc
     2461 gccacgatcc ggccctggcg gaaccccacg gcatgatact cgcgcacgcc gcccgccggc
     2521 acgacgggtg cgacccagcg ggccttgatc agcgtgtcga ctttctccat gctcgaagac
     2581 tctccaggcg gcggccgcgg cctcgcaacg gcctgccgaa gccacccatt ctagcgtccc
     2641 gcgcccgggc gaacgagttg gcagtcgtga cagaccggca gaaacagatc gaaccgctcg
     2701 accccaccgg catccacgcc gtcacctggc gggacggacg gctcgacctg ctcgaccagc
     2761 gtcggctgcc ggccgacgtc cggcgggtcg tatgcgagga cgtggatgcg gcgattcaca
     2821 gcatccgcga catggtggtg cgcggcgcgc cggcgatcgg catcacggcg ggctacatcg
     2881 cggtgctggc ggcccgcaac aggatcgccg ccggggaagc cgactggctg gcgggctttc
     2941 ggcaggacat ggtccgcctg cgcaaggcgc ggccgactgc ggtgaacttg gcctgggcgg
     3001 tggaccgcat gctggcggcc gcggcgtcgg ccggcagcga tcccgtggcc ggcctgctgg
     3061 ccgaagcgcg gcgcatccat gccgaggaca tcgagatgaa cctcgccatg gcggcgcgcg
     3121 gcgccgacct catcgagacg cggggcagcg tgatcaccca ctgcaacgca ggcgcgctgg
     3181 ccaccggcgg cgtcggcacg gcgctcggcg tgatccgcga ggcatggcgc cgcggcctcg
     3241 tcacgcgcgt ctgggtggac gagacccggc cgtggctgca gggcgcacgg ctgacggcct
     3301 gggaactggg gcgcgacggc atcccggcca cggtgatcac cgattccgcc gccgcccagg
     3361 tcatgcgcag cgagtcgccg agctgggcga tcgtgggcgc ggaccgggtt gccgccaacg
     3421 gcgacgtcgt caacaagatc ggcacctacg ggctggccgt ggcggcgcgc caccacggcg
     3481 tgcgcttcat ggtggtcgcg ccgagctcga cgctggacgc tgccaccgca tccggcgacg
     3541 aggtcgagat cgagcagcgc gatccggccg agatctgggc ggccgccggc ggcgtgccgg
     3601 ctggagccga cgcgcgcaat gcggcgttcg acgtgacccc ggcggcgctg atcgacgccc
     3661 tggtgaccga acgcggcgtt gccgcacccc ccctcgggcc cgctctcgag gcgctgctgc
     3721 ggggctgaca gcacgtctcc gggccgtttg cgcggccccc ctgtggtacc attgcacgct
     3781 tgtatgcgag gtcaattccg cactgcatca tgcagggcgc ggagtggcat aacttattgt
     3841 ttttaaacgg ccggttaccg catgtctgaa gtcgcaaaag agatcctcca ggtcaacctc
     3901 gaagacgaga tgaggcagtc ctacctcgac tacgcgatga gcgtgatcgt ggggcgggcg
     3961 ctgccggacg tcagggacgg cctcaagccc gtgcaccggc gtgtcctgta cgccatgcgg
     4021 gagctcggca acgactggaa taaggcctac aagaagtcgg cccgcgtggt cggcgacgtc
     4081 atcggtaaat accatccgca cggcgattcc gcggtgtacg acaccatcgt gcgcatggcg
     4141 cagccgttct cgatgcgcta cgtgctggtg gacgggcagg gcaacttcgg ctcggtcgac
     4201 ggcgactccg ccgcggccat gcgctacacc gaggtgcgca tggcgaaact ggcgcacgaa
     4261 ctgctcgccg acatcgacaa ggagaccgtc gacttcgtcg ccaactacga cgagtcggag
     4321 catgagccgt cggtgctgcc gacgcgggtg ccgaacctgc tggtcaacgg ctccgccggc
     4381 atcgcggtgg gcatggccac caacatcccg ccgcacaacc tgcgcgagat cgtcaacgcc
     4441 tgcatcgccc tgatcgacga cccggaattg agcatcgacg ggctgatgca gcacgtgccc
     4501 ggcccggatt tcccgaccgc cggcgtgatc aacggcgcct ccgggatcgt cgaggcctat
     4561 cgcaccggcc gcggccgcgt ctacatacgc gcccgggcgc acttcgagcc attccgcgac
     4621 ggcagccgcg aggccatcgt gatcacggag ctgccgttcc aggtcaacaa ggcgcggctg
     4681 gtcgaaaaga ttgccgaact ggtgcgcgag aagaagctcg agggcatcag cgagctgcgt
     4741 gacgagtcgg acaaggacgg cctcagggtc gtcatcgagc tgaagcgcgg tgagaacggc
     4801 gaggtggtgc tgaacaacct gttcaagcac accccgctgc agaccgtgtt cggcatcaac
     4861 atggtggcgc tggtcgacgg ccagccgcgg ctgctcaacc tcaaggagat actcgaagcc
     4921 ttcatccgcc atcgccgtga ggtcgtcacc cggcgcacca tcttcgagtt gcgcaaggcg
     4981 cgcgaccgcg cccacgtcct cgaggggctg gcggtggcgc tggccaacat cgacgaggtg
     5041 atcgcgctca tcaaggcctc gccgtcgtcg gccgaggcgc gcgttgccct gaccgcgcgt
     5101 ggctgggccc cgggcgtcgt caccgggctg ctggaacgcg ccggcgcgga ggccacgcgg
     5161 ccggccggcc tggccgcgga attcgggctg ggcgcgggcg gctaccgcct ctccgaggcc
     5221 caggcccagg ccattctcga tctgcgcctg caccgcctga ccgggttgga gcaggagaaa
     5281 ataattaagg aatacgaaga gataatcgag aaaattaagg attattctga gattctcgag
     5341 agcccggatc ggctctcggc agtgatccgc gaggagctca tcgcggtgcg cgacgattac
     5401 ggcgacgacc gccgcaccga gatcctgcgc aaccatctcg acctgtcgat ggaggacttg
     5461 atcagcgagg aggacgtcat cgtgacgctc tcgcacggcg gctacgccaa ggcccagccg
     5521 gtgaccgact accaggcgca gcggcggggt ggccgcggcc gggcggccac gcgggtcaag
     5581 gaagaggatt tcgtcgacaa gctgttcatc gccaacaccc acgactggct gctgtgcttt
     5641 tccagtcgcg gcaaggtgta ctggctgaag gtctacgagc tgcccatcgc cgggcgtggc
     5701 tcgcgcggca agccgatcgt caacctgctg ccgctgggcg aaggcgagcg catcaacgcg
     5761 gtactgccga tccgcgagtt catcgagggg ccgtacgtgt tcatggccac cagccgcggc
     5821 accgtcaaga agacgccgct ggtggcgttc tcgcggccgc ggcgcgacgg catcatcgcg
     5881 atcgacctgc gcgacgacga ttacctgatc gacgtcggca tcaccgacgg gcgccagcag
     5941 atcatgctgt gcgcgtcgaa cggcaaggcg atccgcttca aggaggccga cgtgcgcccg
     6001 atgggccgga ccgccggcgg ggtgcgcggg ctgaagctgg ccgacgagca ccaggtcatc
     6061 gcgttgatca tcgccgacga aggccacttg ctgatcgcga cggagcacgg ctacggcaag
     6121 ctcacgccgg tcgaggattt cccggtccac ggccgcggcg gccagggcgt catcgccatc
     6181 cagaccaacg agcgcaacgg gctgatggtg ggtgcgttgc tggtgcagcc cgatgaccag
     6241 gtgatgatga tttccagcac cggggtgctg gtgcgcacca ccgtggaagg catccccgca
     6301 ctgacccgca acacccaggg cgtgcgcctg atccggctcg acgagggcgg ccggctggtc
     6361 ggcatgggcc gcgtggccgc cgatcaggcc gacgacctcg acgacgacgg cggcgacgca
     6421 ggcgacggcg agaacggcaa cgaataaccg cgaacatcac ggagaatcaa catggatcgt
     6481 gtcttcaact tcagcgcggg tccggcggcc attccgctgc ccgtcctgga gcgcgcgcgc
     6541 gacgaactga ccgactggcg cggctgcggc atgtcggtga tggaattgtc ccatcgcggc
     6601 aaggaattca tgtcgatcgc ggagcgggcc gaggcggacc tgcgacggct gctcgatatt
     6661 ccggccggct accgcgtgct gttcctgcag ggcggcgcat cggcccagtt cggcgcggtg
     6721 ccgttcaatc tcgccgggcc ggattcggtg gcggactacg tggtcaccgg ttcatggtcg
     6781 aagaaggcgc tgggcgaggg ccgccgcttc ctcgaggcgc gcgtcgcggc ggattcggcg
     6841 gacaatcgcc acaccgcgat tcccgacccg gcgagctggc ggctcagccc ggacgcggcc
     6901 tacctgcact acacgccgaa cgagaccatt ggcggggtcg agttccactt cgtgcctgct
     6961 gcgtcggcgc cgctggtggc ggacatgtcc tcgacgatct tgtcgcggcc gctggacgtg
     7021 agccgcttcg gcctgatcta tgccggcgca cagaagaaca tcggcccggc gggactggcg
     7081 atcgtcatcg tgcgcgagga cctgctgggt cgcgcccgcg aaggcacgcc ggtgatgatg
     7141 gattacggcc agcaggccga gaacggctcc atgctcaaca cgccgccgac ctggggctgg
     7201 tacgtcgccg gcctggtgtt cgagtggctg ctggaacagg gtggcctggc cgagatggag
     7261 cggcgcaacc gccacaaggc cgagacgctt tatgcggcca tcgacggctc cgacttctac
     7321 gccaacccgg tggcgaagca ctgtcgctcc tggatgaacg tgcccttcac cctggcggac
     7381 ccggcgctcg acgcccggtt cctggagcag gccaaagccg ccgggctctt gaccctgaag
     7441 ggccaccgtt cggtcggcgg catgcgcgcc agtctctata acgccatgcc gcaggagggc
     7501 gtcgacgcgc tggtggcgtt catgcgggag ttcgagcgca gtcacggtta atccgtctgt
     7561 tgcattgccg cataaatctg ccactatcgg cggatgtgac ccgcgccgct ggaggcgacc
     7621 atgacgttca agattctcac cctcaacaac atcgccctgg ccgggctcga gcgcctgccg
     7681 cgcagtctct acgaagtcgc gtcggaaatc ggccacccgg acgccgtcat ggtgcgttcc
     7741 gcggacatgc acaagaccga ggtgccggcc agcgtcaagg cgatcggccg cgccggtgcc
     7801 ggcgtgaaca acatcccggt ggaggagatg agccggcggg gcgtgccggt gttcaacgcg
     7861 cccggcgcga atgccaatgc ggtcaaggag ctggtcctcg gcggcatgct gctggccgcg
     7921 cgcaacctgt gtgaatcctg ggactacgtg cgcaccctcg acggcagcga ggacgagctg
     7981 caccgtgccg tcgagagcgg caagaagcgc ttcgtcggct tcgagctgcc gggccgcacg
     8041 ctgggcgtca tcggtctcgg cgcgatcggc gtcgaggtcg ccaacgcggc gctcagcctc
     8101 ggcatgcagg tgatcggctt cgacccgaag atgacggtac agcgggcctg gcagctgaac
     8161 tcgggcgtgc gccaggccat gagcctcgac gacctgttca gccgcgcgca ggtggtgagc
     8221 gtgcacgtgc cgctcgcgga aggcaccagg gcgctggtca acgccgaccg tctcgcgctg
     8281 atgaagcccg gcggcgtgtt gctgaacttc tcgcgcggcg ccatcgtcga cgacgctgcg
     8341 gtgctggcct cgctggacga gggcaggctc gcgcgctacg tcactgattt tccgtccatg
     8401 atgaacaagg ggcattcgaa ggtgatcgcc ctgccgcatc tcggcgcctc cacccgcgag
     8461 gcggaggaga actgcgcgat catggtcgcg gaaaacctgc gcgacttcct cgagaacggc
     8521 aacatccgtc actcggtgaa cttccccgag gccatcctgc cgaggatcga gcgcaatacg
     8581 cggatcgcca tcgccaacgc caacgtaccc aacatggtcg gccagatttc cacctcgctg
     8641 gccgaggcgg ggctgaacat ttccgatctg ctcaacaagt cgcgcggtga cgtcgcctac
     8701 accttggtcg acgtcgacgg caaggtgccg ggggaagtcg tggagaagct ggcggcaatc
     8761 gagggcgtgt tgtcgttgcg ggtcattccc gacgccgcgt gacgtgccga tgagcgacgc
     8821 gccggccaac gacatgctcg aagacatccg gcggcggatc gacgccatcg acgagcaatt
     8881 gcaggcgctg atcagccagc gcgccgagct ggcgcgcgcg gtcggggcga ccaagttccc
     8941 ggaaccggcg ggcctgtcga acgcgccgcg cgattactac cgcccggagc gcgaggcgga
     9001 gatcctgcgc aaggccgtgg cgcgcaactc cggcccgctc aaggacgagg agatcgtgcg
     9061 gctgttccgc gagatcatgt cggcctgcct cgcccagcag gagccgatga aggttgcctt
     9121 tctcggcccc gagggcactt tttcccaggc ggccgtgctc aagcacttcg gccattccgt
     9181 gcggaccctc gcggtgcctt ccatcgagga agtgtttcac gacgtcgagg cgggcatcgc
     9241 ggatttcggc gtcgtgccgg tggagaactc caccgagggt acggtcagca ccaccctgga
     9301 catgctggtc gtcacgccgt tgcgcatcag cggcgagatc gagctgcgca ttcgccagca
     9361 cctgctcggc cggatggccg gtctcgacga agtccggcgc gtctattctc accagcagtc
     9421 gctcgcccaa tgtcgcgcct ggctcgacca gcggctgccc ggggtggagc aggtggcggt
     9481 cgcatccaac gccgaggccg cgcggcttgc agccagggaa gcgggcgccg ccgcgatcgc
     9541 cggcgacacg gcggccgagg tctacgggct ggccgtgctg atggcgaaca tcgaggatcg
     9601 cccggacaac accacgcgct tcgtggtcat cggccgcgac atgcccgggc ccagcggccg
     9661 cgacaagact tccctgctgt tgtcctcgcg gcatgccgcc ggtgcgctgc aggaattgct
     9721 ccatccgctg tcgaaacaca acgtaagcat gacgcggatc gaatcgcgcc cctcgcgccg
     9781 cggcaagtgg gactatgtgt tcttcgtcga cgtggaaggg caccgcgaag acgcgcacgt
     9841 cgcggcggcc ctgcgggagc tggagaccgc ggctgcatgg tgccgcgtgc tgggctccta
     9901 tcccaccgcg atcctgtaaa cttccggcct ccgttcggga gggtccatga cagacatcta
     9961 cctcgacctg gccgcgccgg gcattcgcgg cctcaagcct taccagccag gcaagccgat
    10021 tgccgagctg cagcgcgagt acggcgtgtc ggacatcgtc aagctggcgt ccaacgagaa
    10081 tccgctcggg ccggcaccgc gggcgctgac cgccatcgcc gcggccgcgg tcgagctggc
    10141 gcgctatccg gacgccaacg gcttcgagct gaaacaggcg ctggccgacc ggcacgcggt
    10201 gggtcccggg caagtgacgc tgggcaacgg ctcgaacgac gtgctggtgc tgctggccga
    10261 gagcttcctc ggcccgggtc tggaggccgt ttattcgcgg cacgccttcg ccgtatacgc
    10321 gctggcggtg caggcgacgg gcgccgtcca tcgcgtggcg gaggcactgc cggccggtgg
    10381 accgcagccg ctgggccacg acctggacgc catggccgag ctggtgggac cgcggacccg
    10441 catggtgttc gtcgccaatc cgaacaaccc gaccggcacc tgggtagacg cagcggcgct
    10501 ggagcgcttc gtggcgggac tgcccaggca cgtcgtcgcg gtcgtcgacg aggcgtactt
    10561 cgaatacgtg caggcaccgg actatccgga tgccacgcgc tggctggacc gcttccccaa
    10621 cctggtggtg acccgtacct tctccaaggc ctacggcctg gccggactgc gcatcggcta
    10681 cagtgtctcc agcgcggcga tcgccgacat ccttaatcgc atccgccagc cgttcaacac
    10741 caactcgctc gcgcaggcgg cggcattggc cgccctcgac gataccgcgc acctggctcg
    10801 cagcgtcgaa ctgaacacgg cggagatggc ccgcgtcgcg accgcctgtc gttcgctggg
    10861 gctcggcgag aacccctcgg tgggcaattt cctgctggtg gacatgggcg ggccggcgca
    10921 gccgctgttc gacgccttgc tgcgtgaggg cgtcatcgtc cggccggtgg ccaactacgg
    10981 cttgccgcaa cacctgcgca tcagcattgg cctgccggcg gagaacgacc ggctgatcgc
    11041 cgcgctcgag aaggtgacgc ggcaatgaat gcgcccgcat ggcgggtaag tcccggcggc
    11101 acgctggccg gccagctgca gattcccggc gacaagtcgg tgtcgcaccg cgtgatgctg
    11161 ctgggcgcca tcggttcggc cccactcgag gcgcacggct tcctgcgcag cgaggactgc
    11221 ctggcgacgc gacgggccgt cgaggcgctc ggcgcccggg tcgaggacct cgcgaacggc
    11281 ggggtacgcg tagcgccggc ggcggcgctg cgctcaccgg ccgaaccgct ggatttcggt
    11341 aactccggca ccggcatccg gctggccacc ggcctgctgg cggggcaccg ggtggacgcg
    11401 gtgctcaccg gcgacgcctc gctgtgccgt cggccaatgg agcgggtggc cgagccgcta
    11461 cgccgcatgg gcgcgctgct ggccacgagc cacggttgcc cgccgatccg cgtctccgcg
    11521 gccgggccgc tgcggccgct ggactacacc atgccggttg ccagcgccca ggtgaagtcc
    11581 gccctgctgc tggccggact gtccgcggag ggccgcacgg taatccgcca gccggccgtt
    11641 tcccgggatc acaccgagcg cttgctggcc gcgctgggct gtccggtgga gagcggcgaa
    11701 tggggggcgg cagtgaccgg tccggcccaa ccccgcggcg ggcgcgtgac ggtgcccggc
    11761 gatttttcct cggcagcgtt tctcatcgtg ggcggattgc tggcggcggg ggatgcgccg
    11821 ctcgagctgc tcggggtggg catgaacccc acccgcaccg gcttgctgga cattctcacc
    11881 atgatgggcg gacgggtaga gatcacccgc ctgcgggaag aatgcggcga gccggtggcg
    11941 gacctgcggg tctggcgcag cgaactccag ggagtcgacg taccgcccgc cctggtgccg
    12001 ctggcgatcg acgagtttcc ggccctgttc gtggccgcgg cgctggcgcg gggcacgacg
    12061 cgcgtgcgcg gcgcggagga gttgcgcgtc aaggagtcgg accggattgc ggtgatggcg
    12121 cgcggcctgg cggcggtcgg cgtacaggtc gcggagcggg ccgacggcct gttgatcgag
    12181 ggcggccggc tggcgggcgg cacggtggac agcggcggcg accaccgcgt ggcgatgtcc
    12241 ttcgcggtgg cggcggcgcg ggcggaggcc gccatcgaga tcctcgatgt ggccaacgtc
    12301 gcgacttcct tcccgggctt cgccgcggcc gcggcgtcgc tcgggctgca gatcgagacc
    12361 ctgcgggcag gggcggccgc atgagcgccg cgccggtgct ggcggtggac ggacccggcg
    12421 gtgccggcaa gggcaccttg tgccggcgtg tggcggaaca gctgggctgg cagctgctgg
    12481 acagcggcgc actgtaccgg ctggttgcag tggcggccgc cgacgccggg acagcgctcg
    12541 acgatgagcc cgggctcgcg gcgctggcgc gcgacctgcc ggtccggttt gccggcgaac
    12601 gcgtgctgct gggcggccgg gacgtgagcg ccgagatccg ctcggagacc tgcggcgagc
    12661 gggcctcgcg ggtggcggcg ctgccggccg tgcgcaccgc gctggtgggg ctgcagcgga
    12721 gctttcggcg cccgcccggc ctggtcgccg acgggcgcga catgggcacc gtgatctttc
    12781 cggacgcgga gttgaaggtc ttcctgaccg cgtccgccga ggaacgggcg atgcgccggt
    12841 ataagcagtt gaaggaaaag gggtttgatg ttaatcttcg cgacctttcg gcagaggtcg
    12901 cggcccggga caggcgggac gcggagcggg ccgtggcgcc gttgcggccc gcgcccgacg
    12961 cggtcgttct ggacagcacc gaactgagca tcgaccaggt ggtcgaggcg gtgctggacg
    13021 aactgcagcg gcgcggcctg gggccgacag gttgaattcg gttttcgcat gcggtcccga
    13081 cggagcgggg gccttttttc ttagatagcc cggcgtggca gggatgcgat gtcggtgtac
    13141 cactggtcgg cctgcgggcc gttggatcaa caacccatgt cagaaagttt cgcagagctc
    13201 ttcgagcaaa gttttgccag tcagcagatt
//
LOCUS       DAOMPG010000044        12755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2119_length_12755_cov_4.574094, whole genome shotgun sequence.
ACCESSION   DAOMPG010000044 DAOMPG010000000
VERSION     DAOMPG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_12755_cov_4.574094"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLS34_03830"
     CDS             <1..841
                     /locus_tag="PLS34_03830"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRP86633.1"
                     /translation="TGPETGVLIHNGGDVWRSVREGPVKRWGGILLIGVLAAIILFHL
                     VAGSVKLEQRTGRTITRWSLFERVIHWYTAILFIILAITGLSLLFGRTVLIPVMGKEA
                     FAAWAGLAKPVHDWLAVPFMIGLVLMMVPWIKDSLPKAYDWTWIKMGGGYLDKSKHPP
                     AGFVNAGEKIWFWLLFFGGIVLCASGVFLLFPNLGFERSAMQLASVLHAVSGVALIAF
                     SLGHIYLGTLGNEGSLEGMITGEVDEAWAKAHHNLWYDEVKKQGTRPATAAPPHGAGQ
                     ATT"
     gene            946..1647
                     /locus_tag="PLS34_03835"
     CDS             946..1647
                     /locus_tag="PLS34_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRP86634.1"
                     /translation="MRTALLTCVAALGVFVGAAAAAPAAPPVWDGNLETLGEFTAPSA
                     SGKTELMQAAGDGVLERVTALLEAGAEIDERNANGGTALMYAVSYNHLDVVRLLLEEG
                     ADPNAQARIGWTPLLVAAAKGRAEAARLLLEAGAAPAARDAYGWTPLMRAVSGGYIDA
                     VNELLASGEADLEAREESGATALHIAAGRGFTRIVARLLDAGAQRLAADGQGRTPADV
                     ARMLGHTDVEALLRT"
     gene            complement(1669..2418)
                     /locus_tag="PLS34_03840"
     CDS             complement(1669..2418)
                     /locus_tag="PLS34_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRP86635.1"
                     /translation="MSGDPVSRRGFLKLCASTGALVAGSPSLLANATPERVPSPPATL
                     VRPDGRPLRLEDLVVGDSYVFHYPYVSTPCFLINLGKPALPQDSLELDDGRRYRWRGG
                     VGPQRSVVAFSAICAHRMSYPTPTVSFIHYRHGPMAFTDKELNATQREQVIYCCSEGS
                     VYDPAEGCRVLGGPAPQPLAAIDLVLDERTGELRATGTYGGDMFERFFERFGFQLALQ
                     QRKDEEDIRALVTDTTEVVPLREYSANTNVC"
     gene            complement(2496..2897)
                     /locus_tag="PLS34_03845"
     CDS             complement(2496..2897)
                     /locus_tag="PLS34_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRP86636.1"
                     /translation="MNTVVTHNPADALVTPRLRREYRTMECMVRIWCANQHGERAQAA
                     PCEECREFLAYAARRLEKCPYGEAKPTCAKCPIHCYKRAQREHARVVMRYAGPRMTWR
                     HPWLALMHLVDKLRKVEHPMAERRRKSNRVQ"
     gene            3001..3282
                     /locus_tag="PLS34_03850"
     CDS             3001..3282
                     /locus_tag="PLS34_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86637.1"
                     /translation="MTDKTQKAAAGRQDHPFEVSSITRAEAPEGAGSATWYRYEIAQG
                     HNEIVGHREGSLKSVTEAVAEIVLRLNERRLGKSGRVHLTPGPKKNRGG"
     gene            complement(3305..4309)
                     /locus_tag="PLS34_03855"
     CDS             complement(3305..4309)
                     /locus_tag="PLS34_03855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRP86638.1"
                     /translation="MDRQTDNSLAYQDLQPDDILDSVESLGLRCDGRLQALNSYENRV
                     YQVGIEDGAPVVVKFYRPGRWSDAAILEEHGFAYELAARDIPVVAPLRHAGSSLHRHR
                     DFRFAVSPRVAGRIPELDDLRLLRQVGRLVARLHLCGQAARFEHRPRLTPRLLGDASR
                     DFLLDGGVIPAHVVQAYESLCADLLDAVNGAWARAGARELRLHGDFHPGNVLAQDGQV
                     HVVDLDDTCTGPAVQDLWMFLSGDRHEQEPQLAELLAGYLEFREFDPAELQLIEALRA
                     LRIMHYAAWLARRWEDPAFRAAFPWFGTARYWEDHVLSLREQLALLQDPPLDWRPPAP
                     "
     gene            4472..5101
                     /locus_tag="PLS34_03860"
     CDS             4472..5101
                     /locus_tag="PLS34_03860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HRP86639.1"
                     /translation="MGSSLRDAACDPSRRAFLRASGALSGGAWLALQFPGLAAAAEAA
                     AAARDAAAAFRHLAGTEAAALEALAARIIPDGETPGAREAGVIHFIDQALGGFMAEAA
                     GELQRGAQSLDELAAAAGRPFAELDAVAQDTILRGIEDTPFFGLMQFLTVAGMFSLPS
                     YGGNRHYAGWKLLGFEHRHGWLPPFGHYDAAATGVQEHGQDSGADHGHG"
     gene            5094..6755
                     /locus_tag="PLS34_03865"
     CDS             5094..6755
                     /locus_tag="PLS34_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRP86640.1"
                     /translation="MAEQPLFATRRPVDFVIVGSGSAGGILARELAVAGFEVVVLEQG
                     RYRRTAEFNHDELAVMFNHEFVGGPHGGDPQSFRRNAADTATVPEFPPAYYARMVGGS
                     SVHFSANFWRLRPVDFREGGLFGGIAGTDLVDWPIDYDELEPYYTRVDWEIGVSGAPG
                     PFDPPRSRPYPLPPMPLKSSGALLEKGARKLGLHPQVAPVAILSQPYNGRAPCIHCGF
                     CMGFGCETGAKSSTLVTMIPQAEATGRCEIRPGSTVHRLETGADGRISEVLYYDAEGR
                     EHGQRARAVIVAANGAETPRLLLMSASERFPHGLANGSGYVGRNLMFNAHSGTHAWFD
                     EPLHDYKSVQCSRIVHDFYESDPRRGFYGGGGIDARPFLAASPMLYALTAMPPDAPTW
                     GAGYKEALAHGFTRNMFLAASTTSLPVPTNTITLDPDRKDRFGRPSLCVTYHDHRDDL
                     ATAAFLQDWAVGIAEAAGAAKVWRNPVQPETVGAHLLGTCRMGDDPTRSVVDRFHRSH
                     EVANLFICDGSSMVTSGRGQPTMTIMALAFRAAQHIAGFARRGEI"
     gene            6769..7212
                     /locus_tag="PLS34_03870"
     CDS             6769..7212
                     /locus_tag="PLS34_03870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRP86641.1"
                     /translation="MIRRFILGLAVLVAGGALAADLEFHPMASDPDGRLPFSEAVRAG
                     DLLFLSGMLGIRPGSPGLVPGGIQPETRQALENIRAAVERHGAAMERIAKCTVFLADV
                     GEWAAMNEVYVEFFPAGKPARTALGVGGLPLGGRVEIECIAVFPD"
     gene            complement(7277..7531)
                     /locus_tag="PLS34_03875"
     CDS             complement(7277..7531)
                     /locus_tag="PLS34_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86642.1"
                     /translation="MKPTEWITEGMAMAANFPLDAQWRFAVAALSLFCMAGLLALLPE
                     IPELQLIWRAARVVVPIIKVRRVLAGRRQDWRGLLLDVLT"
     gene            7822..9204
                     /locus_tag="PLS34_03880"
     CDS             7822..9204
                     /locus_tag="PLS34_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP86643.1"
                     /translation="MSFDSLGLQAELLRAVAEEGYDTPTPIQQQAIPVILAGRDVLAG
                     AQTGTGKTAGFTLPMLQMLAAKPVEGRRRIRALVLVPTRELAAQVAASIETYGRHLPR
                     LSCAVVFGGVSINPQIATLRRGVDILVATPGRLLDHAQQRTVDLSSVEFLILDEADRM
                     LDMGFIRDIRRVLAMLPKRRQNLLFSATFSDEIRSLANGLLDAPAQIEVARRNTPTEL
                     TTQVVHPVDRSRKRELLSHLMRSGDWRQVLVFTRTKHGANRLATQLESDGISAAAIHG
                     NKSQAARTKALADFKAGKVRALVATEVAARGLDIDQLPHVVNFELPHVAEDYVHRIGR
                     TGRAGSEGEAVSLVCVDERRLLTDIERLLGKDIPRRMVAGFEPDPSIRPEPIENGRRP
                     GGAAARSAMVAGRGDRARRIEEFAPEGNSAGVALCVGRPLLLYRNNALLYGMSKRYEY
                     LCWKSGLQRD"
     gene            9164..9454
                     /locus_tag="PLS34_03885"
     CDS             9164..9454
                     /locus_tag="PLS34_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRP86644.1"
                     /translation="MNIYVGNLAYSVTEDDLRTAFSEFGSVTRATVITDKFSGQSKGF
                     GFVEMANSSEAEAAIRGLNDKPLNGRNIKVNQAKPREERPARSGGGGGGGRW"
     gene            9587..9988
                     /locus_tag="PLS34_03890"
     CDS             9587..9988
                     /locus_tag="PLS34_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86645.1"
                     /translation="MTDQDSGAGMNAADLYLEEIFTDRRVGTIQRLTPVDADGRPDSG
                     RTVSYVGQAQVMTPAGALPLSFEIPAASLSEAVAGFGAAAERAVQETMQRLQEMRREA
                     AGSIIVPESGGAGLGGGLPGMSGGGGKIRMP"
     gene            10000..10755
                     /locus_tag="PLS34_03895"
     CDS             10000..10755
                     /locus_tag="PLS34_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRP86646.1"
                     /translation="MTYCVAIAVEQGLVFASDSRTNAGPDQVSVFGKMHVLLEEPGRF
                     FVLLSAGNLGTTQAVRTRIRHDLEFDRRPNLKTVQSMAEAAEYLGAINAEQRRKHAEG
                     GESDTAMTATFILGGQIAGRAPGILLIYPEGNYIRASSQTPFLQIGELKYGKPILDRL
                     VRPELPLDDTARVALLSMDATMRSNVTVGPPIEVLIYDPRKPRALRHMVFGPDDDYLR
                     ELGRSWQGYVEKAFSVLPPLPPRPTELLRVVDE"
     gene            complement(10694..11731)
                     /locus_tag="PLS34_03900"
     CDS             complement(10694..11731)
                     /locus_tag="PLS34_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HRP86647.1"
                     /translation="MKLFSCRCGARVFFDDYWCTACGADLGYAPGLAMFLARDAGGPE
                     PFLAADGSAWKACRNRVLYNACNWLLPADDPAAFCQACRLNSVVPNLSLEHNVRLWRR
                     IEHAKRRLVYGLLQLGLPLDAAPGRPPLRFEFLEDQRSNPEVAESLVMTGHRAGIITI
                     NLLEADDVARHAVREQMSERYRTLLGHFRHESGHYYEALLVPDGAARDEYRALFGDER
                     ADYSAALARHHEAGPAPDWSERCVSSYASSHPMEDWAECWSHYLHITDGLETARATGF
                     VTDPPSADWESEIALWISVSVRLNELARGLGVDDPYPFVLNTPVRAKLAFIHRRLGAT
                     PSAAAAMAAAR"
     gene            complement(11728..12663)
                     /locus_tag="PLS34_03905"
     CDS             complement(11728..12663)
                     /locus_tag="PLS34_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRP86648.1"
                     /translation="MLSRVAERMYWAARYLERAENTARLVRVYDAALLDLPEQAGVGW
                     PILVDICGARETFTSRYPKGGDRETLKFLISDPDSPSSIVASLSQARENFRTTRDLCP
                     TEAWRSVNELYLFGRKHLPKMASPRRRHEVLSGIVQHAQQLTGLLSGTMSHGDAYSFI
                     CLGRNLERADMTTRIIDVAAATLLRSAVSEARLDNTLWMAVLKSLSGYQMYRQYVRRR
                     IRAEDVINFLVGDPLFPRAVTHCLIELEAAMHALPQHEGPLRAVLRLQRMVDAQQPEA
                     VEEAALRRLMDELQLELAGIHGQVSAAWFLPEHAG"
BASE COUNT         1997 a         4319 c         4425 g         2014 t
ORIGIN      
        1 cacggggccg gagaccggcg tcctgatcca taacggcggt gacgtctggc gctcggtccg
       61 cgaagggccg gtgaagcgct ggggcggcat cctgctgatc ggggtgctgg cagccatcat
      121 cctgttccac ctggtagccg gctccgtgaa gctggagcag cgcaccggca ggacgatcac
      181 acgctggtca ctgttcgagc gggtgatcca ctggtacacg gcgatcctgt tcatcatcct
      241 cgccattacc ggcctgagcc tgctgttcgg gcggacggtg ctgatcccgg tgatgggcaa
      301 ggaggcgttc gcggcctggg ccggcctcgc gaagccggtg catgactggc tggcggtgcc
      361 cttcatgatc ggcctggtgc tgatgatggt gccgtggatc aaggacagcc tgcccaaggc
      421 ctacgactgg acctggatca agatgggcgg cggctacctc gacaagagca agcatccgcc
      481 ggccggtttc gtcaacgccg gcgagaagat ctggttctgg ctgctgttct tcggcggtat
      541 cgtgctgtgc gcctcgggcg tgttcctgct gttcccgaac ctcggcttcg agcgcagcgc
      601 gatgcagctg gccagcgtcc tgcatgccgt cagcggcgtc gccctgatcg ctttctcgct
      661 gggtcatatc tacctgggta cgctcggcaa cgagggttcg ctggaaggca tgatcaccgg
      721 cgaggtcgac gaggcctggg ccaaggcgca ccacaacctg tggtacgacg aggtcaagaa
      781 gcagggtacg cgtccggcca ccgcggcacc gccgcacggt gcgggtcagg cgacgaccta
      841 gccgtcaggc tctgcagctc gacaacgacg ggagcgcttc ggcgctcccg tttttttgtg
      901 aaatgctact ggccttacgc gcgccgagac ggcatgcttt gcggcatgag gacagccctg
      961 ctgacatgtg ttgccgcgct cggcgtgttc gttggcgccg ccgcggcagc gccagccgct
     1021 ccaccggtgt gggacggcaa cctcgagacc ctcggcgagt tcacggcgcc gtcggcgagc
     1081 ggcaagaccg agttgatgca ggcggccggc gatggcgtgt tggagcgcgt cacggcgctg
     1141 ctcgaggccg gcgccgagat cgacgagcgc aatgccaacg gcggcaccgc actgatgtac
     1201 gcagtgtcct acaaccacct cgacgtggtg cggttgttac tggaggaggg ggccgacccc
     1261 aacgcccagg cgcgcatcgg ctggacgccc ctgctggtgg cggcagccaa gggacgggcg
     1321 gaggccgcgc ggctgctgct cgaggcaggg gctgcgcccg cggcgcgtga cgcctatggc
     1381 tggacgccgc tgatgcgtgc ggtctcgggt ggctacatcg acgccgtcaa cgagctgctc
     1441 gcctcgggcg aggcggatct ggaggcgcgc gaggagtccg gcgccacggc gctgcacatt
     1501 gccgccgggc ggggcttcac gcgaatcgtc gcgcgcctgc tggacgcagg ggcgcagcgc
     1561 ctggcggccg acggacaggg gcgcacgccg gccgacgtcg cgcgcatgct cggtcacaca
     1621 gacgtcgaag ccctgctgcg aacctaggcg gccacgcagc cggcggggct agcagacgtt
     1681 ggtgttggcg ctgtactcgc gcagcgggac gacctcggtg gtgtccgtga cgagcgcccg
     1741 gatgtcctcc tcgtccttgc gctgctgcag cgccagctgg aagccgaagc gctcgaagaa
     1801 gcgctcgaac atgtcgccgc cgtaggttcc tgtcgcccgc agctcgccgg tccgttcatc
     1861 cagcaccagg tcgatggcgg cgagcggctg cggcgccggg ccgccgagca cgcggcagcc
     1921 ttcggccgga tcatagacgc tgccctcgga gcagcagtag atcacctgct cgcgttgcgt
     1981 ggcgttgagc tccttgtccg tgaaggccat cggcccgtgc cggtaatgga tgaagctcac
     2041 ggtgggcgtc gggtagctca tgcggtgcgc gcagattgcc gagaacgcca ccaccgagcg
     2101 ctgcggcccg acgccgccgc gccagcgata gcggcgcccg tcgtcgagct cgaggctgtc
     2161 ctgcggcagc gccggtttgc ccaggttgat caggaagcag ggtgtgctga cgtacgggta
     2221 atgaaacacg taactgtcgc cgaccacgag gtcctcgagg cgcagcggcc ggccgtccgg
     2281 gcgcaccagg gtcgctggcg gggacgggac acgctcgggc gtggcgttgg ccagcaggga
     2341 agggctgccg gccaccagcg cgcccgtgct ggcacaaagt ttaaggaatc cgcggcggga
     2401 aacggggtct ccggacatgt ctgtcacctg ccaaatcgca ccgtgaacga tgctagcatt
     2461 cgccacgcgc gaggcaacag tttcgagtca cccggctact gcacccggtt ggacttgcgg
     2521 cggcgctcgg ccatcgggtg ctccaccttg cgcagcttgt ccaccaggtg catcagcgcc
     2581 agccagggat gccgccaggt catccgcggc cccgcatagc gcatgaccac gcgcgcgtgc
     2641 tcgcgctgcg cacgcttgta gcagtgaatc gggcacttgg cgcaggtcgg cttggcctcg
     2701 ccgtaggggc acttttcgag ccgacgcgcg gcgtaggcga ggaattcgcg gcactcctcg
     2761 cagggcgccg cctgggcgcg ctcgccgtgc tggttggcgc accagatacg caccatgcac
     2821 tccatggtgc ggtactcccg ccgcagccgg ggcgtcacca gcgcgtcggc gggattatgc
     2881 gtcacgaccg tattcatgcg gactatgttc atctttaggc cggactccat caataggcgt
     2941 tctccgcagc gccggcgacc ccggcgggca cggtgacggc agatggagta agctgcaccg
     3001 atgactgaca agacccagaa ggccgccgcg gggcggcagg accacccctt cgaggtgtcg
     3061 tccatcacgc gtgccgaggc gcccgagggc gccggctcgg ccacctggta ccgctacgag
     3121 atcgcgcagg gacacaacga gatcgtcggc caccgcgaag gcagcctgaa atcggtgacc
     3181 gaggcggtgg ccgagatcgt gctgcggttg aacgagcgcc ggctcggcaa gtcaggtcgc
     3241 gtgcacctga cgcccggccc gaagaagaat cgcggcggct gagcccgttt tcgtccgacc
     3301 ggcctcaggg cgccggtggt cgccagtcca gcggcgggtc ctgcagcagc gccagctgct
     3361 cgcgcaagga cagcacgtgg tcctcccagt aacgggccgt gccgaaccag ggaaaggcgg
     3421 cgcgaaaggc cggatcttcc cagcggcgcg ccagccacgc ggcatagtgc atgatccgca
     3481 gcgcgcggag cgcctcgatc agctgtagct cggcggggtc gaactcccgg aactcgaggt
     3541 agcccgccag cagctcggcg agctgcggct cctgctcgtg ccggtcgccg gacaggaaca
     3601 tccacaggtc ctggacggcc gggccggtgc aggtgtcgtc gaggtccacg acatggacct
     3661 gcccgtcctg cgccagcaca ttgcccggat ggaagtcccc gtgcagccgc aactcgcgcg
     3721 cgcccgcccg cgcccaggcc ccgttcaccg cgtccagcag gtccgcacac aggctctcgt
     3781 aggcctgcac gacatgcgcc gggatgacgc cgccgtcgag caggaaatcg cgcgaggcgt
     3841 cgccgagcag gcgcggcgtg aggcgcggcc ggtgctcgaa acgggccgcc tggccgcaca
     3901 ggtgcagccg cgccaccagc cgccccacct ggcgcagcag ccgcagatcg tccagttcgg
     3961 gaatgcggcc cgccacgcgc ggactcaccg cgaaacggaa gtcccggtgg cgatggaggc
     4021 tgcttccggc gtggcgcagc ggcgccacca ccgggatgtc gcgcgccgcc agctcataag
     4081 cgaatccgtg ctcctcgaga atcgccgcgt ccgaccagcg gcccggccgg taaaacttga
     4141 ccaccaccgg cgcgccgtcc tcgatgccca cctggtagac ccggttttcg tagctgttga
     4201 gggcctgcag ccggccgtcg cagcgcagcc cgaggctctc cacggagtcg aggatgtcgt
     4261 ccggctggag atcctggtaa gcgagcgagt tgtccgtttg cctgtccact ggtgtcgcct
     4321 gcgggtcggg ccgcgactat agcagcatga aattggggac attccccaat tcggacgcga
     4381 attggggaat gtccccaatt ttgctggcgg cgggaattga atgcgactac cttgcaatgg
     4441 ttcgttcacg aacagggccg ggggagcgta catgggttcg agtcttcgag acgcggcgtg
     4501 cgatccttcg cgccgcgcat tcctgcgggc gtccggcgcg ctgtctggcg gcgcctggct
     4561 ggcgctgcag tttcctggcc tggcggcggc ggccgaggct gcggcggcgg cacgcgatgc
     4621 cgcggccgcc ttccggcatc ttgccggcac cgaggccgct gcgctggagg cccttgctgc
     4681 gcggatcatc cccgatggcg agacgcccgg cgcccgcgag gccggcgtga tccacttcat
     4741 cgaccaggcg ctcggagggt tcatggccga ggctgccggc gagctgcagc ggggcgcgca
     4801 gtcgctcgac gagctggcgg cggctgcagg ccggcccttc gccgagctcg atgccgtggc
     4861 ccaggacaca atcctgcgcg gcatcgagga cacgccgttc tttggcctga tgcagttcct
     4921 gaccgtggcg ggaatgttct cgttaccttc atatggcggc aaccgccact acgccggctg
     4981 gaagctgctc ggattcgagc atcgacacgg ctggttgccg cctttcgggc attacgatgc
     5041 cgccgcgacc ggtgtgcagg agcacggcca ggactccggc gcagatcacg gccatggctg
     5101 agcaaccatt gttcgccact cgccggccgg tcgatttcgt catcgtcggc tcggggtccg
     5161 ccggcggcat cctggcgcgc gagctggcgg tggccggctt cgaggtcgtg gtgctggagc
     5221 agggacgcta tcgacggacg gcggagttca accacgatga actcgccgtg atgttcaacc
     5281 acgagttcgt cggcggcccg cacggcggcg acccgcagtc cttccggcgc aacgccgccg
     5341 ataccgccac ggtgcccgag tttccgccgg cctattacgc gcgcatggtg ggcggcagca
     5401 gcgtgcactt ctccgcgaat ttctggcggc tcaggccggt cgatttccgc gaaggcgggc
     5461 tgttcggcgg catcgccggg acggacctcg tcgactggcc catcgactac gacgaactcg
     5521 agccctacta cacgcgcgtg gactgggaga tcggcgtgtc cggcgcgccg ggccccttcg
     5581 atcccccgcg ctcccgtccc tacccgctgc cgcccatgcc gttgaaatca tccggcgcgt
     5641 tgctggagaa gggcgcccgc aagctcggcc tgcacccgca ggtggcgccg gtggcgatcc
     5701 tgtcgcagcc ctacaatggc agggcgccct gcatacactg cggcttctgc atgggtttcg
     5761 gctgcgagac tggcgccaag tcgtccacgc tggtcaccat gatcccgcag gccgaggcaa
     5821 ccggccgctg cgaaatccgt cctgggtcca ccgtgcaccg cctcgagacc ggggccgacg
     5881 gccgcatcag cgaggtgctg tattacgacg cggagggccg cgagcacggc cagcgggcca
     5941 gggcagtcat cgtcgccgcc aacggcgcag agacgccgcg gctgctgctg atgtccgcct
     6001 ccgagcgctt cccccacggt ctcgccaacg gcagcggcta cgtcggccgc aacctgatgt
     6061 tcaacgcgca ctccggcacg catgcctggt tcgacgagcc gctgcacgac tacaagagcg
     6121 tgcagtgcag ccgcatcgtg cacgacttct acgagtccga cccgcggcgt ggcttctacg
     6181 gcggcggcgg catcgacgcg agaccgttcc tggcggcctc gccgatgctt tatgcgctca
     6241 cggcgatgcc gccggacgcg ccgacctggg gcgccggcta caaggaggcg ctggcgcacg
     6301 gcttcacccg caacatgttc ctggccgcct cgacgacgtc actgccggtg ccgaccaaca
     6361 ccatcaccct cgacccggac cgcaaggacc gcttcggccg cccgtcgctg tgcgtgacgt
     6421 accatgatca ccgcgatgac ctggcgacgg cggcgtttct gcaggactgg gccgtcggca
     6481 ttgcggaggc cgcgggcgcc gcgaaggtct ggcgcaaccc cgtgcagccg gaaaccgtcg
     6541 gcgcgcacct gctcggcacc tgccgcatgg gcgatgatcc gacccgctcg gtggtggatc
     6601 gctttcaccg cagccacgag gtggcgaacc tgtttatctg cgacgggtcg agcatggtga
     6661 cctcggggcg cggccagccg accatgacca tcatggcgct ggcctttcgc gccgcgcagc
     6721 acatcgccgg ctttgcccgg cggggcgaga tctgaaggag acacaagcat gatccgacgt
     6781 ttcatcctcg gcctggcggt gctcgtcgcc ggcggcgcgc tggcggccga cctcgagttc
     6841 catcccatgg cgagcgaccc ggatggccgg ctgccgttct cggaggcggt gcgcgccggc
     6901 gacctgttgt tcctctcggg catgctcggc atcaggccgg gctcgcccgg gctcgtgccg
     6961 ggcgggatcc agccggagac ccgccaggcg ctcgagaaca ttcgcgcggc cgtcgagcgc
     7021 cacggcgccg ccatggagcg gatcgcgaag tgcaccgtgt ttctcgccga cgtgggcgag
     7081 tgggcagcga tgaacgaggt ctatgtcgag ttcttcccgg ccgggaagcc ggcgcgcacc
     7141 gcgctgggcg ttggtggcct gccgctcggc gggcgcgtcg agatcgagtg catcgccgtc
     7201 ttccccgact gaaaccggtg tctgaccccg ttttaaactg gggtcagaca ccggtttcca
     7261 gcctgcggcg ggcgcctcaa gtcaggacgt ccagcagcag cccgcgccag tcctgccggc
     7321 gtccggccag cacgcgccgc accttgatga tcggcacgac cacccgggcc gcccgccaga
     7381 tgagttgcag ttccgggatt tccggcagca acgccagcag tccggccatg cagaacaggc
     7441 tcagggccgc gaccgcgaag cgccactgcg cgtccagggg aaagttggcc gccatggcca
     7501 tgccctcggt gatccattca gtcggtttca tgtcagcctc ctccggccgg ggcatccggc
     7561 cttgggaggc gagtctggac gggcgatgta atccgacgat ttcgcaactg caagagattg
     7621 tcaccggatt gtttctgcaa attaatcctt tgcatcgccg ggacttgtgc tatagtccgc
     7681 gcactcctga tgcacaccgg tctgcatccg gcccttgtgg cacgcctctt tcggtcagcc
     7741 tcgagcacct cttaattcga gcctggaccg gccggagcaa agcttccggt gggcgttaca
     7801 cgatcaggag atcacgtttc tatgtcattt gattccctcg gcctccaggc cgagcttctt
     7861 cgtgcagttg ccgaagaggg ctacgacacc ccgaccccca tccagcagca ggccattccc
     7921 gtcatcctgg caggccgcga cgtgctggcc ggcgcccaga cgggcactgg caagacggcc
     7981 ggtttcacgc tgccgatgct gcagatgctg gccgccaagc cggtcgaagg ccggcggcgc
     8041 atccgcgcgc tggtgctggt gccgacccgc gaactggccg cccaggtggc cgccagcatc
     8101 gagacttacg gccgccacct gccgcgcctg agctgtgccg tggtgttcgg cggcgtcagc
     8161 atcaacccgc agatcgccac cctgcgccgc ggcgtcgaca tcctcgtcgc cacgccgggc
     8221 cggctgctcg accatgccca gcagcgcacg gtggacctgt cgtccgtcga gttcctcatc
     8281 ctcgacgagg cggatcgcat gctcgacatg ggcttcattc gcgacatccg cagggttttg
     8341 gccatgctgc cgaagcgtcg ccagaacctg ctgttctccg ccaccttctc ggacgaaatc
     8401 cgctcgctgg ccaacggcct gctcgacgcg cccgcccaga tcgaggtcgc gcggcgcaac
     8461 acgccgactg agctgaccac ccaggtcgta cacccggtgg atcgcagccg caagcgcgag
     8521 ctgctgtcgc acctgatgag atcgggcgac tggcgccagg tgctggtgtt cacgcgtacc
     8581 aagcatggcg ctaaccggct cgccacgcag ctggaaagtg acggcatctc cgccgcggcc
     8641 atccacggca acaagagcca ggccgcgcgc accaaggcgc tggccgactt caaggccggc
     8701 aaggtgcggg cgctggtggc gaccgaggtc gcggcgcgcg ggctggacat cgaccagttg
     8761 ccgcacgtgg tgaatttcga gctgccgcat gtcgccgagg actacgtgca ccgcatcggc
     8821 cgcaccggcc gcgcgggcag cgaaggcgag gccgtctcgc tggtgtgcgt ggacgagcgg
     8881 cgcctgctga ccgacatcga gcggttgctc ggcaaggaca ttccgcgccg gatggtggcg
     8941 ggtttcgagc ccgatccctc gatccggccg gagccgatcg aaaacggccg aaggcccggg
     9001 ggcgcggcgg cgcggagcgc catggtcgcc ggtcgcgggg accgcgccag gagaatcgaa
     9061 gaatttgccc ctgagggcaa ctcggcaggg gtcgctttgt gcgttggccg accgctgctg
     9121 ctgtatcgca acaacgcact cttgtatgga atgagcaaac gttatgaata tctatgttgg
     9181 aaatctggcc tacagcgtga ctgaagacga tctgcggacc gccttctccg agttcggaag
     9241 cgtcacccgt gccaccgtca tcacggacaa gttctcgggc cagtccaagg gcttcggctt
     9301 cgtcgagatg gcgaacagct ccgaggccga ggctgccatt cgcgggctga acgacaagcc
     9361 cctgaacggc cgcaacatca aggtcaacca ggccaagccg cgcgaagagc gccccgcgcg
     9421 tagcggtggt ggcggcggcg gcggtcgctg gtaggaccgt cgagcaaaga tcgggccttc
     9481 gggttcgttc tgcggaaggg gccccgaaag gggccctttt cgtttcccgg cctgccggaa
     9541 cttgctaagg ttcgcagcca gaaattcccc gcaacaggac caagtcatga cagaccagga
     9601 ttcgggcgcc ggcatgaatg ccgccgacct gtacctcgaa gagattttca ccgatcgccg
     9661 ggtgggcacc atccagcgcc tgacgccggt ggatgccgac ggccggcccg acagcggccg
     9721 cacggtgtcc tatgtcggcc aggcgcaggt gatgacgccc gccggcgccc tgccgctgtc
     9781 cttcgagatt cccgcggcgt cgctcagcga ggccgtggcc ggcttcggcg cggccgccga
     9841 gcgcgccgta caggagacca tgcagcgcct ccaggagatg cggcgtgagg ccgcaggctc
     9901 gatcatcgtg ccggagtcgg gcggcgcggg gcttggcggc ggcctgccgg gcatgtcggg
     9961 cggcggcggc aagatccgca tgccctgagg gcgtcatcca tgacctactg cgtcgcgatc
    10021 gccgtcgaac agggcctggt cttcgcctcg gattcgcgta ccaacgccgg cccggaccag
    10081 gtcagcgtgt tcggcaagat gcacgtgcta ttggaagagc cgggccgttt cttcgtattg
    10141 ctgtccgcgg gcaacctcgg caccacgcag gcggtccgga cccgcatccg gcacgacctc
    10201 gagttcgacc gtcgacccaa cctgaagacc gtgcaaagca tggcggaggc ggccgagtat
    10261 ctcggcgcga tcaacgcgga gcagcggcgc aagcacgcgg agggaggcga gtcggatacg
    10321 gcgatgaccg ccacgttcat cctgggcggc cagatcgccg ggcgggcgcc cggcatcctg
    10381 ctgatctacc cggaaggcaa ttacatccgg gcctcgagcc agacgccgtt cctgcagatt
    10441 ggcgagctca agtacggcaa gcccattctc gaccggctgg tgcggccgga gctgccgctc
    10501 gacgacactg caagggtggc gctgctgtcg atggacgcca ccatgcgcag caacgtcacc
    10561 gtggggccgc cgatcgaggt gctgatctac gacccgcgca agccgcgcgc gctccggcac
    10621 atggtgttcg gtcccgacga cgattacctg cgggagctcg gccgcagctg gcagggctat
    10681 gtcgagaagg ccttcagcgt gctgccgcca ttgccgccgc ggccgacgga gttgctccga
    10741 gtcgtcgatg aatgaaggcc agcttggcgc gcaccggcgt attcagcacg aaggggtagg
    10801 ggtcgtcgac gccgagcccg cgcgccagct cgttcaggcg caccgacacg gaaatccata
    10861 gcgcgatctc gctctcccag tcggcggacg gcgggtcggt gacgaagccg gtggcgcgcg
    10921 ccgtctccag cccgtcagtg atgtgcaggt agtgggacca gcactccgcc cagtcctcca
    10981 tcgggtggct ggacgcatag gagctgacac agcgctcgct ccagtcgggg gcgggtccgg
    11041 cctcgtgatg gcgggcgagc gcggccgaat agtctgcccg ctcgtcaccg aacagcgcgc
    11101 ggtactcgtc cctggccgcg ccgtccggca ccagcagcgc ctcgtagtag tggccgctct
    11161 cgtggcggaa atgccccagc aaagtgcggt aacgctccga catctgctca cgcaccgcgt
    11221 ggcgggccac gtcgtccgct tccagcaggt tgatggtgat gatacccgcg cggtgcccgg
    11281 tcatgaccag cgattcggcc acctcgggat tgctgcgttg gtcctcgagg aactcgaaac
    11341 gcagcggcgg gcgtcccggc gcggcatcca gcggcaggcc cagctgcagc aggccgtaga
    11401 ccagccgtcg cttggcgtgt tcgatgcgtc gccagagcct gacgttgtgc tcgagggaca
    11461 ggttcgggac gacactgttg agacgacagg cctggcagaa ggcggccggg tcgtcggccg
    11521 gcagcagcca attgcaggcg ttgtacagca cgcggttgcg gcaggccttc caggcgctgc
    11581 catcggccgc gaggaaaggt tcggggccac ccgcatccct ggcgagaaac atggccaggc
    11641 cgggcgcata gccgagatcg gcgccgcagg cggtgcacca gtagtcgtcg aagaatacac
    11701 gcgcaccgca gcggcagctg aacagcttca tccggcatgc tccggcagga accaggctgc
    11761 cgagacctgc ccatggatgc cggccagctc gagctgcagc tcgtccatga gccgccgcag
    11821 agcggcctcc tcgacggcct ctggctgctg cgcgtcgacc attcgttgca gccgcagcac
    11881 cgcgcgcagc ggcccttcgt gttgcggcag cgcgtgcatc gcggcttcga gctcgatgag
    11941 gcagtgcgtg acggcgcgtg ggaacagggg atcgccgacc aggaagttga tcacgtcctc
    12001 ggcacggatc cgacggcgca cgtactggcg gtacatctgg taacccgaaa gcgacttcag
    12061 cacggccatc cacagggtgt tgtcgaggcg cgcctcggat accgcggagc gcagcagcgt
    12121 ggcggcggcg acgtcgatga tgcgcgtggt catgtcggcc cgctcgagat tgcggccgag
    12181 gcagatgaag ctgtaggcgt cgccgtggct catggtgccg gacagcagcc cggtgagctg
    12241 ctgcgcgtgt tgcacgatac cggaaagcac ctcgtggcgg cgccgcggcg aggccatctt
    12301 cggcaggtgt ttgcggccga acaggtagag ctcgttgacg ctgcgccatg cctcggtcgg
    12361 gcacaggtcg cgcgtggtgc ggaagttctc ccgcgcctgc gacagcgagg cgacgatgga
    12421 gctcgggctg tcagggtccg agatcaggaa cttgagcgtc tcgcgatcgc cgcccttcgg
    12481 gtaacggctg gtgaaggtct cgcgtgcacc gcagatgtcc accaggatcg gccagccgac
    12541 accggcctgc tccggcaggt cgagcagcgc cgcgtcatag acgcgcacca ggcgcgccgt
    12601 gttttccgcg cgctccaggt agcgggcggc ccagtacatg cgctcggcga ctcgtgacaa
    12661 catgctcagg cctccccggt ctcgacgacc caggtgtcct tgctgccgcc gccctggctg
    12721 gagttgacca ccagcgagcc cttcaccagc gcgac
//
LOCUS       DAOMPG010000045        12674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2135_length_12674_cov_4.192963, whole genome shotgun sequence.
ACCESSION   DAOMPG010000045 DAOMPG010000000
VERSION     DAOMPG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_12674_cov_4.192963"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLS34_03910"
     CDS             complement(<1..423)
                     /locus_tag="PLS34_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRP86649.1"
                     /translation="MSRVLLIADHDGKTLSTGIARSVACARQLGNVHVAVFHAPDADA
                     PCAAAAKLDGVSRVLSVGNPANGAPLAAVLAPQIAQLAREHEGGYDYVLAPSTTFGKD
                     VLPRVAALLGVPQVSDLMEVVDGRTFKRPIYAGNVITTV"
     gene            complement(420..1169)
                     /locus_tag="PLS34_03915"
     CDS             complement(420..1169)
                     /locus_tag="PLS34_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004086235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRP86650.1"
                     /translation="MKILVGIKRVIDYNVRVQVSRDGTGVVKDGVKMSINPFDEIAVE
                     EALRMKERGDAAEVIVVSIGPEAAQQQLRTALAMGADRAILFQSEEEIQPLAAARALL
                     ALIKQEDPGLVLLGKQAIDDDANQVGQMLAALWDRPQATFVSRLEFSNGVAKATREVD
                     AGLENIEVELPAVVTVDLRLNEPRFVKMPDIMKAKRKPLDIKPFAELGVATPPQLETG
                     LHEPPPKRQGGTRVESVEALVKALRDKGVLA"
     gene            complement(1182..2843)
                     /locus_tag="PLS34_03920"
     CDS             complement(1182..2843)
                     /locus_tag="PLS34_03920"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRP86651.1"
                     /translation="MSEQDTGIERDVMAYDFAIVGGGPAGLACAIRLKERLPDASVCL
                     LEKGSEIGAHILSGCVMEPGPLDELMPGWRDQPPAICVPAGRDEFRLLTRGGSWRLPT
                     PPQMHNAGNFIVSLGELCKQLAAHAESLGVEVFPGFPARRPLLAGEGAETRVIGVRCG
                     DMGREADETPDGRPGDNFAPGVDIHAGTTILAEGCRGSVSKELIARFHLDRDATPQTY
                     GLGFKELWQLPPGRTTPGLIQHTVGWPLDSATYGGSFVYHLDQDRLYVGFVVGLDYAD
                     PRFSPFEAFQQFKHHPDLLELLEGGEIISSGARTIIEGGLQALPKMDMPGALLIGDAA
                     GTLNVPKIKGVHQALRSGMLAAEHLAATGAPAGYDAAFRASPAGRELHKVRNIRPGFH
                     RGLWLGMANGALETLLGGRSPWTLRTHADWDSLRTLASARAHGEQAAMAAHWHAERTL
                     PPRDRLASVYFANTAHEESQPVHLRVADTSICATRCAEEFGNPCTRFCPVGVYEMVAD
                     GHGGRRLQINASNCVHCKTCDIKDPYQIIDWVTPEGGSGPNYQTM"
     gene            complement(2895..3662)
                     /locus_tag="PLS34_03925"
     CDS             complement(2895..3662)
                     /locus_tag="PLS34_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86652.1"
                     /translation="MELSKLKAVVSGGASGLGHAVAKRIVAEGGQVAILDVNETQGRE
                     AAAALGERARFFRTDVSNDAEVIASVDAAAQAFGGINLAVSCAGILGAGRVLGREGPM
                     AGEFFAKVILVNLVGTFQLAKAAASHMQHNEPNAEGERGVIVNTASIAAFEGQVGQAA
                     YSASKAGVAGMALPLAREFARLGIRVNTIAPGIFLTPMMEGMPEHVQQSLAAQVPFPS
                     RLGRPEEFADLVAVICTSPMLNAETIRLDGAIRMQPK"
     gene            complement(3694..4593)
                     /locus_tag="PLS34_03930"
     CDS             complement(3694..4593)
                     /locus_tag="PLS34_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRP86653.1"
                     /translation="MPTHVKVVEVGPRDGLQNEQAVVPPAVKIEFIERLAAAGLPAVE
                     ATSFVSPRWIPQLADADEVFRGISRRPGVAYPVLVPNLKGLERAAAAGVEEIAVFTAA
                     SETFNQRNINASVAESLARIRALAAPALERGMRIRGYVSCVLGCPYEGTVDEARVIEV
                     AQALLELGCYEVSLGDTIGVGTPVAARRLLWAVAGECGMERLAVHFHDTRGQALANVF
                     ACLEAGVTVVDASVAGLGGCPYAQGASGNVATEDLVYQLHGLGIETGIDLPALVAAGH
                     FICRHLGREPGSRLGRLPEYRRD"
     gene            complement(4601..>6361)
                     /locus_tag="PLS34_03935"
     CDS             complement(4601..>6361)
                     /locus_tag="PLS34_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRP86654.1"
                     /translation="HPGYGFLSENAAFARDCVAAGLVFVGPRHETIRAMGSKSEAKAI
                     MEDAGVPVVPGYHGADQAPARLAERAAAIGYPVLIKAAAGGGGKGMRVVRSADDFAEA
                     LAAAKREANKAFGDDQVILERFIERPRHIEVQVFGDRHGNVIHLWERECSTQRRYQKV
                     IEESPSPLLDDATRQRMSEVAVEAARAVEYLNAGTVEFIVSPEREFWFMEMNTRLQVE
                     HPVTELLTGLDLVEWQLRVAAGEPLPLAQAEIPRRGHAVEARIYAENPANNFMPSTGR
                     IRRFRAPDRHPAIRLDSGFEEGDDVSHHYDPMLAKLVVHGLDRRECVTRLREALAHTA
                     IAGVTTNLPLLRAVAAHPAFEAGEVDTGFLDRELEEVLWNVPAPPLPVLAVAAWRIAR
                     DLPRPAAAADRWSPWGAGDGWRAAGAGGVPVALLDAAGRRHELRLELVAGGARVHSGD
                     HHVDALLEDLGGGRLRLECQGIAGDALVAAGDDRVYVGFGDHGWDFVVEPLHADASGH
                     GEEDTHPVAPMPGTIVALKVAVGDRVVVGQPLLIMEGMKMELTLAAPVAGRVERVLCS
                     EGDSVEADAVLVDILPDEAD"
     assembly_gap    6362..6371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6372..6588)
                     /locus_tag="PLS34_03940"
     CDS             complement(<6372..6588)
                     /locus_tag="PLS34_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRP86655.1"
                     /translation="MSHELRLRRLLIANRGEIACRVMRSCRRLGIETMAVHSSADARA
                     RHVREADTAVAIGGAAPADSYLNGARII"
     gene            complement(6585..7397)
                     /locus_tag="PLS34_03945"
     CDS             complement(6585..7397)
                     /locus_tag="PLS34_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRP86656.1"
                     /translation="MTSNVLLETDARGVATLTLNRPELHNAFDAALVARLTELLIEIK
                     SRQDIRVVVLTGAGRSFSAGADLNWMRAMAGCSEEENIEDALHLADLMSLLDTLPLPT
                     IARVNGHAYGGGVGLIACCDIAIASNEARFALSEVRLGLVPAVISPYVIAAIGARNAR
                     RLFLSAEAMNAKLARRVGLVHEIAKPNKLDAALDDQVEMLLRGGPRALRESKELVFTV
                     EGGGISADHALKRRTAQIIAQLRVSAEGQEGLAAFLQKRAPAWAPARDEDGG"
     gene            complement(7408..9018)
                     /locus_tag="PLS34_03950"
     CDS             complement(7408..9018)
                     /locus_tag="PLS34_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRP86657.1"
                     /translation="MPVLSTAVRPDSEEFNANRRHMEALVADLRAQLAQVARGGGERA
                     RDRHAGRGKLLPRERVDALLDAGSPFLELSALAAHGLYDGQAPAAGIITGIGRVAGRE
                     VVIVANDATVKGGTYFPITVKKHLRAQEVALENHLPCIYLVDSGGAFLPLQDEVFPDR
                     DHFGRIFYNQARMTAANVPQLAVVMGSCTAGGAYVPAMCDEAVIVRNQGTIFLGGPPL
                     VKAATGEEVDAETLGGGDVHSRISGVTDHLANDDAHALAIARELVATLNRVKPTHGEL
                     AEPVAPLYPAEDLYGIVPRDTRYPYDVREVIARIVDGSVLTEFKPLYGKTLVCGFARV
                     HGYPVGILANNGILFSESALKGAHFIQLCNRRGVPLLFLQNITGFMVGRKYEHAGIAK
                     DGAKMVNAVATATVPKFTVVIGGSFGAGNYAMCGRAYGARFLWTWPNARISVMGGEQA
                     ASVLATVRRDGLAARGDSWPEEDEASFRAAIREQYETQGHPYYASARLWDDGVIDPAD
                     TRRVLALALSASRNAPPAAEGPYGIFRM"
     gene            complement(9024..10202)
                     /locus_tag="PLS34_03955"
     CDS             complement(9024..10202)
                     /locus_tag="PLS34_03955"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRP86658.1"
                     /translation="MSNDSIVIAAARRTPIGAFQGGLGTVPAPQLAATAIRAALADAG
                     LAPGEVSEAIIGCVLPAGIGQAPARQAALAAGIPDAVGCTTVNKVCGSGMKAVMFGHD
                     LLRAGSAKVVLAGGMESMSNAPYLLPKARAGYRMGHQEVLDHMFYDGLQNPYDGNMMG
                     HFAELTADKYGFTREAQDAFSIDSVQHALTAVDGAFDTEIAPVTVKSRRGEVVVGRDE
                     EPGRCEIDKIPTMRPAFRKDGTVTAASSSSISDGAAALLLMTETEAARRGLEPLARIV
                     GHGGFAHAPEWFTTAPVAATRQLLERLGWGPDDVDLYEINEAFAVVAMAAIHDLGLDR
                     NKVNVNGGACALGHPIGATGARLLVTLVHALRARGLKRGLASLCIGGGEATAVAIEAL
                     "
     gene            complement(10220..11395)
                     /locus_tag="PLS34_03960"
     CDS             complement(10220..11395)
                     /locus_tag="PLS34_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRP86659.1"
                     /translation="MSVFNTGLGEDLDLLRDAVRRFAAERIAPRAADIDARNEFPQEL
                     WPELGEMGLLGITVGEEYGGAGLGYLAHVVAMEEISRASASVGLSYGAHSNLCLNNIW
                     LNGTDAQKRKYLPKLCSGAWVGALAMSEPGAGSDVIGSMSCRAEQRGDVWVANGSKMW
                     ITNGPEASVLLVYMRTADAAEGSRSITAFIVEKGMPGFRTAQKLDKLGMRGSNTCELV
                     FEDCEIPAENVLGEVGGGAYILMRGLDSERLVLSGGPIGIMQSCMDVVLPYLHERRQF
                     GQPIGTFGIMQAKVADMYTALQSSRAFTYRVAGQFDRGEPTRFDAAACLLLASKSAVD
                     LALEAIQALGGNGYINEYPAGRLLRDAKLYEIGAGTSEIRRMLIGRELFRATGGDAG"
     gene            complement(11400..12521)
                     /locus_tag="PLS34_03965"
     CDS             complement(11400..12521)
                     /locus_tag="PLS34_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRP86660.1"
                     /translation="MNLFQQLDGMGHEQVVFFQHKDSGLKAIVAIHNTALGPALGGLR
                     MWPYASDDEAVKDVLRLSRGMTYKAAVSGLNLGGGKAVLIGDPAKDKSEGLFRAFGRF
                     IGSLGGRYITAEDVGTTVDDMDYIYQETDRVVGVHRVHGGSGDPSPFTAFGTLQGIKA
                     CLLKKYGHDDPGKVSFAVQGVGSVGYYLTKFLSEAGAKVFACDINADRVQHVVDEFGA
                     EAVPLEQIYDVDANVFSPCALGGVINETSLPKLKFDIVAGSANNQLETNAHGTALEER
                     GILYAPDYAINAGGLMNVAIELQGYDRDRAYRTVSSIRDIMGRIFQLAERDGIPTWQA
                     ADRLAEERIAMITKVKVPYSKRFKDRLSGRRTHATAGDE"
BASE COUNT         1804 a         4438 c         4426 g         1996 t
ORIGIN      
        1 gacggtggtg atgacgttgc cggcgtagat cggccgcttg aaggtgcgcc cgtcgacgac
       61 ctccatcagg tcggagacct gtggcacgcc gagcagcgcg gcgactcgcg gcaggacgtc
      121 cttgccgaag gtggtggagg gcgccagcac gtagtcgtag ccgccctcgt gctcgcgggc
      181 cagctgcgcg atctgcggcg ccagcacggc cgcgagcggc gcgccgttgg caggattacc
      241 gaccgacagc acccgcgaca cgccgtcgag cttcgcggcc gccgcacagg gcgcgtccgc
      301 atccggcgca tggaacaccg ccacgtgcac gttgccgagc tgccgcgcgc aggcgaccga
      361 gcgcgcgatc cccgtgctca gagtcttgcc gtcgtggtcg gcgatcaaca ataccctgct
      421 catgccagca ctcccttgtc cctgagcgcc ttgaccagcg cctcaaccga ttcgaccctg
      481 gtgccgccct ggcgcttggg cggcggctcg tgcaggccgg tctcgagttg cggcggcgtc
      541 gccacgccga gctcggcgaa gggcttgatg tcgagcggct tgcgcttggc cttcatgatg
      601 tcgggcattt tcacgaagcg cggctcgttc agcctgaggt cgacggtgac gaccgccggc
      661 agttccacct cgatgttctc caggcccgcg tcgacctcgc gggtcgcctt cgccacgccg
      721 ttgctgaact cgagcctgga cacgaaggtc gcttgcggac ggtcccacag cgcggccagc
      781 atctggccga cctggttggc gtcgtcgtcg atggcctgct tgcccagcag caccagccct
      841 ggatcctcct gcttgatgag cgccagcagc gcgcgcgccg cggcgagcgg ctgaatctcc
      901 tcctccgact ggaagaggat tgcgcggtcc gcccccatgg cgagcgcggt gcgcaattgc
      961 tgctgggccg cctccggccc gatcgagacg acgatcacct ccgcggcgtc accacgctcc
     1021 ttcatgcgca gcgcttcctc gacggcgatt tcgtcgaagg ggttgatgct catcttgacg
     1081 ccgtccttga cgacgccggt gccgtcccgg ctcacctgga cccggacgtt gtaatcgatg
     1141 acacgcttga tgccgaccag gatcttcatc tcgtttcctt gtcacatggt ctggtagttg
     1201 ggcccgctgc cgccctcggg cgtcacccag tcgatgatct ggtacgggtc cttgatgtcg
     1261 caggtcttgc agtgcacgca gttggatgcg ttgatctgca ggcgccggcc gccgtgcccg
     1321 tcggccacca tctcgtagac gcccaccggg cagaagcgcg tgcagggatt gccgaattcc
     1381 tcggcgcagc gcgtcgcgca gatcgaggtg tcggccaccc gcaggtgcac cggctgggac
     1441 tcctcgtgcg ccgtgttggc gaagtacacg ctggccaggc ggtcgcgcgg tggcaaggtc
     1501 cgctcggcgt gccagtgcgc ggccatcgcc gcctgctcgc cgtgcgcgcg cgcactcgcc
     1561 agcgtccgca ggctgtccca gtccgcgtgt gtcctgagcg tccaggggct gcggccgccg
     1621 agcagggtct cgagcgcgcc gttggccatg cccagccaca ggccgcgatg aaagcccggg
     1681 cggatgttgc gcaccttgtg cagctcgcga ccggccggcg aggcgcggaa agcggcgtcg
     1741 taacccgccg gcgcgcccgt ggccgcgagg tgttccgccg ccagcatccc ggagcgcagc
     1801 gcctggtgca cgcccttgat cttcggcacg ttgagcgtgc ccgccgcgtc gccgatcagc
     1861 agcgcgccgg gcatgtccat cttcggcagc gcctgcaggc cgccctcgat gatggtgcgc
     1921 gcgccggagg aaatgatctc gccgccctcg agcaactcga gcaggtcggg atggtgcttg
     1981 aactgctgga aggcctcgaa gggcgagaag cgcgggtcgg cgtagtccag cccgacgacg
     2041 aagccgacgt acaggcggtc ctggtcgagg tgatagacga agctgccgcc ataggtggcg
     2101 ctgtccagcg gccagccgac cgtgtgctgg atcaggccgg gtgtcgtgcg ccccggaggc
     2161 agctgccaca gctccttgaa gccgagaccg taggtctgtg gtgtggcgtc gcggtcgagg
     2221 tgaaagcgcg caatgagttc cttcgagacg ctgccgcggc atccttcggc gaggatcgtg
     2281 gtgccggcat ggatgtcgac gcccggggcg aagttgtcgc cggggcggcc gtccggggtt
     2341 tcgtcggctt cgcggcccat gtcgccgcag cgcacgccga tgacgcgggt ctcggcgccc
     2401 tcgccggcga gcagcggccg gcgggcggga aagccgggga acacctcgac gccgagcgac
     2461 tcggcgtgtg ccgccagctg cttgcagagc tcgcccagcg agacgatgaa attgccggcg
     2521 ttgtgcatct gcggcggcgt cggcagacgc caggagccgc cccgggtcag caggcggaac
     2581 tcgtcgcggc cggccggcac gcagatggcg ggcggctggt cccgccagcc cggcatgagc
     2641 tcgtccagcg gcccgggttc catgacgcag cccgacagga tgtgcgcgcc gatctccgag
     2701 cccttctcga gcaggcacac gctcgcatcc ggcaggcgct ccttcagccg gatggcgcag
     2761 gccagcccgg ccggaccgcc gccgacgatc gcgaagtcgt atgccatgac atcgcgctcg
     2821 atgccggtgt cctgctcgct catggtcgcc tctggccgtg cggggcagcc ttgaatttta
     2881 cctgagccat ctctctattt gggctgcatg cggatcgcgc cgtcgaggcg aatggtctcc
     2941 gcgttgagca tcgggctggt gcagatgacg gcgaccaggt cggcgaactc ctccggccgg
     3001 ccgaggcggg aggggaaggg cacttgcgcg gcgagcgact gctgcacgtg ctccggcatg
     3061 ccttccatca tcggcgtcag gaagatgccc ggcgcgatgg tgttgacgcg gatgccgagg
     3121 cgtgcgaatt cgcgcgccag cggcagcgcc atgccggcca cgccggcctt ggaggcggag
     3181 taggcggcct ggccgacctg gccctcgaag gccgcgatcg acgccgtatt gacgatcacg
     3241 ccgcgctcgc cttccgcgtt gggctcgttg tgctgcatgt gcgaggccgc ggccttggcg
     3301 agctggaagg tgccgaccag gttcaccagg atgaccttgg cgaagaattc accggccatg
     3361 gggccctccc ggccgagcac gcgcccggcg ccgaggatgc cggcgcagct taccgcgagg
     3421 ttgatgccgc cgaaggcctg ggccgctgcg tccacgctgg cgatgacctc ggcatcgttg
     3481 ctgacgtcgg ttctgaagaa ccgcgcgcgt tccccaaggg cggcggcggc ctcccggccc
     3541 tgcgtctcgt tgacgtcgag gatcgccacc tggccgccct cggccacgat tcgctttgcg
     3601 accgcgtggc ccaggccgga ggcgccgccg gagacgacgg ctttgagctt ggagagttcc
     3661 attcaggggc ttcctttggc tacttgtgaa atttcagtca cggcgatatt ccggcagccg
     3721 gccgagccgg ctgcccggct cgcgaccgag gtgacggcag atgaagtggc cggcggccac
     3781 cagcgccggc aggtcgatgc cggtctcgat gccgaggccg tgcagctggt agacgaggtc
     3841 ttcggtggcg acgttgcccg aggcgccctg cgcataggga cagccgccga gcccggccac
     3901 cgaggcgtcc accacggtca cgccggcctc gaggcaggcg aagacgttgg ccagcgcctg
     3961 gccgcgcgtg tcgtggaaat gcaccgcgag gcgctccatg ccgcattcgc cggccacggc
     4021 ccagagcagc ctgcgcgccg ccaccggcgt gccgacgccg atggtgtcgc cgagcgagac
     4081 ctcgtagcag cccagctcca gcagcgcctg ggccacctcg atgaccctgg cctcgtcgac
     4141 cgtgccctca tagggacagc cgagcacgca ggaaacgtag ccgcgaatcc gcatgccgcg
     4201 ctcgagtgcg ggcgcggcga gtgcgcggat gcgcgccagg ctctcggcca cggaggcatt
     4261 gatgttccgc tggttgaacg tctccgaggc tgcggtgaac accgcgatct cctcgacgcc
     4321 cgctgccgcc gcccgttcca ggcccttcag gttgggcacc aggacggggt aggccacgcc
     4381 cggccggcgc gagatgccgc ggaagacctc gtccgcatcg gcgagttgcg gaatccagcg
     4441 cggactgacg aaactggtgg cctcgaccgc gggcagaccg gccgccgcca gccgctcgat
     4501 gaactcgatc ttcaccgccg gcggcacgac cgcctgttcg ttctgcagtc cgtcccgcgg
     4561 gccgacctcc acgactttca cgtgcgtggg catcgacacg tcagtcggcc tcgtccggca
     4621 ggatatcgac cagcacggca tccgcctcga cgctgtcacc ctcgctgcac agcacacgct
     4681 ccacccggcc cgccaccggc gccgccagcg tcagctccat cttcatgcct tccatgatca
     4741 acagcggctg gccgacgacg acccggtcgc cgaccgcgac tttcagcgcg acgatggtgc
     4801 ccggcatggg cgcgaccggg tgggtgtcct cctcgccgtg gcccgacgcg tcggcgtgca
     4861 acggctcgac cacgaaatcc cagccgtggt cgccgaagcc gacgtacacc cggtcgtcgc
     4921 cggcggccac cagtgcgtcc ccggcgatgc cctggcactc gagccgcagc cgcccgccgc
     4981 ccaggtcctc gagaagcgca tcgacatggt ggtcgcccga gtggacccgc gccccgccgg
     5041 cgaccagctc caggcgcagc tcatggcgac gcccggcggc gtccagcagg gcgaccggaa
     5101 cgccgcccgc gcccgccgca cgccagccgt cgccggcccc ccagggcgac cagcgatccg
     5161 ccgctgcagc cgggcgcggc agatccctgg cgatacgcca ggcagccacg gccagcaccg
     5221 gcagtggtgg tgccggcacg ttccacagca cctcctcgag ctcgcgatcg agaaagcccg
     5281 tgtccacctc gcccgcctcg aaggccgggt gcgccgccac tgcccgcaac agcggcaggt
     5341 tggtggtcac gccggcgatc gccgtgtgcg cgagggcttc gcggagtcgg gtgacgcatt
     5401 cgcgccggtc gaggccgtgc acgaccagct tggccagcat cggatcgtag tgatggctga
     5461 cgtcgtcacc ctcctcgaag cccgagtcca gccggatggc cggatggcga tcgggcgcgc
     5521 ggaacctgcg gatgcggccc gtcgagggca tgaaattgtt ggcgggattc tcggcgtaga
     5581 tgcgcgcctc gacggcatgg ccgcggcgtg gaatttcggc ctgggcgagg ggcaggggct
     5641 cgccggcggc gacccgcaac tgccactcga ccaggtccag tccggtgagc agctcggtga
     5701 ccgggtgctc cacctgcagg cgcgtgttca tttccatgaa ccagaattcg cgctccgggc
     5761 tgacgatgaa ctcgaccgtg ccggcattca ggtactcgac cgcgcgcgcc gcctccaccg
     5821 ccacctcgga catgcgctgg cgggtggcgt cgtcgagcag cggggagggc gattcctcga
     5881 tgaccttctg gtagcggcgc tgcgtggagc actcgcgctc ccacaggtgt atcacgttgc
     5941 cgtggcggtc gccgaacacc tgcacctcga tgtgccgcgg ccgctcgatg aagcgctcga
     6001 ggatgacctg gtcgtcgccg aaagccttgt tcgcctcgcg ctttgcggcc gccagcgcct
     6061 cggcgaaatc gtccgcgctg cgcaccaccc gcatgccctt gccgccgccg ccggccgcag
     6121 ccttgatgag caccgggtaa ccgatcgccg cggcgcgctc ggcgagcctg gccggcgcct
     6181 ggtccgcgcc gtgatagccc ggcacgaccg gcacgccggc gtcttccatg atcgccttgg
     6241 cttcgctctt cgagcccatg gcgcgaatgg tctcgtggcg cggccccacg aacaccagcc
     6301 cggccgcgac gcagtcgcgc gcgaacgcgg cgttctccga caggaaaccg tagcccgggt
     6361 gnnnnnnnnn ncgatgatgc gcgcgccatt caggtaactg tccgcgggcg ccgcgccgcc
     6421 gatggcgacg gcggtgtcgg cctcgcggac gtgccgcgca cgcgcgtcgg cgctggaatg
     6481 cacggccatc gtctcgatgc cgagccggcg gcagctgcgc atcacgcggc acgcgatctc
     6541 gccccggttg gcgatcaaca gcctgcgcaa gcgcagttcg tggctcaccc gccgtcctcg
     6601 tcccgcgccg gcgcccaggc cggcgcgcgc ttctgcagaa aggccgccaa gccttcctgt
     6661 ccctccgccg agacgcgcag ctgggcgatg atctgcgcgg tgcggcgctt cagcgcatgg
     6721 tcggcggaga tgccgccgcc ctctacggtg aacaccagtt ccttgctctc gcgcagcgcg
     6781 cgggggccgc cgcgcagcag catttccacc tggtcgtcga gcgcggcgtc gagcttgttg
     6841 ggcttggcga tctcgtgcac caggccgacg cgccgcgcca gcttggcgtt catcgcctcg
     6901 gcgctcagga acaggcggcg cgcattgcgc gcaccgatcg ccgctatgac gtagggactg
     6961 atgaccgccg gcacgaggcc gagccgcacc tcggagagcg cgaagcgagc ctcgttgctg
     7021 gcgatcgcga tgtcgcagca ggcgatcagc ccgacgccgc cgccgtaggc atggccgttg
     7081 acccgcgcaa tggtgggcag cggcaaggtg tcgagcaagg acatcaggtc cgcaagatgc
     7141 agcgcgtcct cgatgttctc ctcttcgctg cagccggcca tggcgcgcat ccagttcaag
     7201 tctgccccgg ccgagaacga gcggcccgcg ccggtgagca cgacgaccct gatgtcctgg
     7261 cgtgacttga tttcgatcag caactcagtg agccgcgcca ccagcgccgc gtcgaaggcg
     7321 ttgtgcagct ccggcctgtt cagcgtgagc gttgcgacgc cgcgggcgtc ggtttccagc
     7381 agtacgttgc ttgtcatggt cctgtcgtca catcctgaaa atgccgtagg ggccctcggc
     7441 ggccgggggc gcgttcctgc tcgccgacag cgccagcgcc agcacgcggc gcgtgtccgc
     7501 cggatcgatc acgccgtcgt cccagagcct cgcactggca tagtaagggt ggccctgggt
     7561 ctcgtactgt tcgcgtatgg cggcgcggaa gctcgcttcg tcttcctcgg gccagctatc
     7621 gccgcgcgcc gccaggccgt cgcgacgcac ggtcgccagc acgctggcag cctgctcgcc
     7681 gcccattacc gagatgcgcg cgttcggcca ggtccacagg aagcgcgcgc cgtaggcccg
     7741 gccgcacatc gcgtagttgc cggcgccgaa ggagccgccg atcacgaccg tgaacttcgg
     7801 caccgtggcg gtggcgacgg cgttcaccat cttcgccccg tccttggcga tgccggcgtg
     7861 ctcgtacttg cgcccgacca tgaagccggt gatgttctgc aggaacagca gcggcacgcc
     7921 gcgccggttg cacagctgga tgaagtgcgc gcccttgagc gcggattcgg agaacaggat
     7981 gccgttgttg gcgagaatgc ccaccgggta gccgtgaacg cgcgcaaagc cgcagaccag
     8041 cgtcttgccg tacagcggct tgaactcggt gagcacggag ccgtcgacga tccgcgcgat
     8101 cacctcgcgc acgtcgtagg gatagcgcgt gtcccggggc acgatgccgt agagatcctc
     8161 ggcggggtac agcggggcca ccggctcggc cagctcgcca tgggtgggct tcactcggtt
     8221 cagcgtcgcg accagctcgc gggcgatcgc cagggcgtgc gcgtcgtcgt tcgccaggtg
     8281 gtccgtgacc ccggaaatgc ggctgtggac gtcgccgcca cccagcgtct cggcgtcgac
     8341 ctcttcgccg gtggccgcct tcaccagcgg cggcccaccg aggaaaatgg tgccctggtt
     8401 gcgcacgatc accgcctcgt cgcacatcgc cggcacgtag gcgccgcccg ccgtgcagga
     8461 gcccatcacc acggcgagct gcggaacgtt ggcggccgtc atgcgcgcct ggttgtagaa
     8521 aatgcggccg aaatggtcgc ggtccgggaa cacctcgtcc tgcagcggca ggaaggcgcc
     8581 gccggaatcc accaggtaga tgcaggggag atgattctcc agcgccacct cctgcgcgcg
     8641 caggtgcttc ttgaccgtga tggggaaata ggtgccgccc ttgacggtcg cgtcgttggc
     8701 gacgatgacg acctcgcggc ccgcgacgcg cccgatgccg gtgatgattc cggctgccgg
     8761 ggcctgtccg tcgtagagcc cgtgggcggc cagcgccgag agctcgagga aagggctgcc
     8821 ggcgtcgagc agggcgtcga cgcgctcccg gggcaacagc ttgccgcgcc cggcatgccg
     8881 gtcccgcgcc cgctcgccgc cgccccgggc gacctgcgcc agctgcgcgc gaaggtcggc
     8941 caccagcgcc tccatgtggc ggcggttggc gttgaactcc tcgctgtcgg ggcgaactgc
     9001 ggtggagagg acgggcatcg cggctagagc gcttcgatgg cgaccgcggt cgcttcgccg
     9061 ccgccgatgc acagcgaggc caggccgcgc ttcaggccgc gggcgcgcag cgcgtggacc
     9121 agcgttacca gcaggcgcgc gccggtggcg ccgatcgggt gaccaagcgc gcaggcgccg
     9181 ccgttgacgt tcaccttgtt gcggtccagc ccgagatcgt ggatggcggc catcgccacc
     9241 accgcgaagg cctcgttgat ctcgtacagg tcgacgtcgt caggtcccca gccgagccgc
     9301 tcgagcagct gccgggtggc ggccacaggg gcggtggtga accactcggg ggcatgcgcg
     9361 aagccgccgt ggccgacgat cctggcaagc ggctcgaggc cgcgccgcgc ggcctcggtc
     9421 tcggtcatca gcagcagcgc ggcggcgccg tcggaaatcg acgacgagga cgcggccgtg
     9481 acggtgccgt ccttgcggaa ggccggccgc atggtcggga tcttgtcgat ttcgcagcgg
     9541 ccgggttcct cgtcacggcc gacgactacc tcgccacggc gcgacttgac cgtcaccggc
     9601 gcgatttccg tatcgaaggc gccgtccacc gcggtcaggg catgttgcac gctgtcgatc
     9661 gagaacgcgt cctgcgcctc gcgggtgaag ccgtacttgt ccgccgtcag ctccgcgaag
     9721 tggcccatca tgttgccgtc gtaagggttc tgcagtccgt cgtagaacat gtggtcgagc
     9781 acttcctggt ggcccatacg gtagccggcg cgggccttgg gcagcaggta gggcgcgttg
     9841 ctcatggatt ccatgccgcc cgccaggacg accttcgccg acccagcgcg caacaggtcg
     9901 tgaccgaaca tgaccgcctt catgccggag ccgcagacct tgttgacggt cgtgcagccg
     9961 accgcgtccg ggatgcccgc cgcgagcgcc gcctggcggg ccggcgcctg gccgatgccc
    10021 gccggcagca cgcagccgat gatcgcctcg gaaacctcgc cgggcgcgag cccggcgtcg
    10081 gccaacgcgg cgcgaatggc cgtggcggcc agttgcggcg ccggcacggt gcccaggccg
    10141 ccctggaagg cgccgatcgg ggtgcggcgc gcggccgcga tgacgatgct gtcgttgctc
    10201 atgtccggat tcctcgctgt cagccggcgt ccccgccggt ggcgcgaaac agctcgcgcc
    10261 cgatcagcat gcggcggatt tcgctggtcc ccgcgccgat ctcgtaaagc ttggcgtcgc
    10321 gcagcaggcg tcccgccggg tactcgttga tgtagccgtt gccgcccagc gcctggatgg
    10381 cctccagcgc caggtccacg gccgatttcg aagccagcag caggcaggcg gcggcgtcga
    10441 agcgggtcgg ctcgccgcgg tcgaactggc ccgccacgcg ataggtgaag gcgcggctgg
    10501 actgcagcgc cgtgtacatg tcggcgacct tggcctgcat gatgccgaaa gtgccgatcg
    10561 gctggccgaa ctgcctgcgt tcgtgcagat acggcagcac cacgtccatg caggactgca
    10621 tgatgccgat cggccccccc gacagcacca ggcgctcgga gtcgagcccc cgcatcagta
    10681 tgtaggcacc gccgccgact tcgccgagca cgttctccgc cgggatctcg cagtcctcga
    10741 acaccagttc gcaggtgttc gagccgcgca tgccgagctt gtccagtttc tgcgccgtgc
    10801 ggaagcccgg catgcccttt tcgacgatga aggcggtgat gctgcgcgag ccctcggccg
    10861 catcggccgt gcgcatgtag accagcagca cggaggcctc gggcccgttg gtgatccaca
    10921 tcttcgagcc gttcgccacc cacacgtcgc cgcgctgctc ggcgcggcag ctcatcgagc
    10981 cgatgacgtc ggagccggcg cccggctcgg acatggcgag cgccccgacc catgcgccgg
    11041 aacagagctt cggcaggtac ttgcgcttct gcgcatccgt gccgttgagc cagatgttgt
    11101 tcaggcagag gttggaatgg gcgccgtagg acaggccgac cgaggccgag gcgcgcgaga
    11161 tttcctccat ggccacgacg tgcgccaggt agccgaggcc cgcgccgccg tactcctcgc
    11221 cgacggtgat cccgagcagc cccatttcgc ccagttcggg ccagagctcc tgcggaaact
    11281 cattgcgggc gtcgatgtcg gccgcccgcg gcgcgatgcg ctcggcggca aaacggcgca
    11341 cggcgtcgcg cagcaggtcg aggtcctcgc ccagcccggt gttgaacacg ctcataggct
    11401 cattcgtcgc ccgcggtggc gtgggtgcgg cggccggaca ggcggtcctt gaagcgcttg
    11461 gagtagggca ccttgacctt ggtgatcatg gcgatgcgtt cctcggcgag ccggtcggcg
    11521 gcctgccagg tggggatgcc gtcgcgctcg gcgagctgga agatgcgccc catgatgtcg
    11581 cggatgctgg acacggtccg gtaggcgcgg tcgcggtcgt aaccttgcag ctcgatggcg
    11641 acgttcatca ggccgcctgc attgatggcg tagtccggcg cgtagaggat gccgcgctcc
    11701 tcgagggccg tgccgtgcgc attggtctcg agctggttgt tggcgctgcc cgcgacgatg
    11761 tcgaacttca gtttcggcag gctcgtttcg ttgatcacgc cgccgagcgc gcagggtgag
    11821 aacacgttgg catcgacgtc gtagatctgc tccagcggca cggcctcggc gccgaactcg
    11881 tcgaccacgt gctgcacgcg atcggcattg atgtcgcagg cgaacacctt ggcgcccgcc
    11941 tcggacagga acttggtgag gtagtagccg acgctgccga caccctgcac cgcgaagctg
    12001 accttgccgg ggtcgtcgtg gccgtacttt ttcaacaggc aggccttgat gccctgcagg
    12061 gtgccgaagg cggtgaaggg cgaggggtcg ccgctgccgc cgtgcacgcg atggacgccc
    12121 accacgcggt cggtctcctg gtagatgtag tccatgtcgt cgacggtcgt gccgacgtcc
    12181 tcggccgtga tgtagcggcc gccgagcgag ccgatgaagc ggccgaaggc gcggaacagg
    12241 ccctcgctct tgtccttcgc cgggtcgccg atcagcaccg ccttgccgcc gccgaggttc
    12301 aggccggaca ccgccgcctt gtaggtcatg ccgcgcgaca ggcgcagcac gtccttgacc
    12361 gcctcgtcgt cgctggcgta cggccacatg cgcaggccgc cgagcgccgg gccgagggcc
    12421 gtgttgtgga tcgcgacaat ggccttcagg ccactgtcct tgtgctggaa aaacacgacc
    12481 tgctcgtggc ccatgccgtc gagctgctgg aacagattca tcaagggaga ccctcctggt
    12541 ttgcggtctg gatcgctgtg tccgcccgtt gcatcgttat tctggtgcgc gcctccccgc
    12601 gggacccgga cggggtcgcg cgcggcaatt ctgcggtgtc gctggtcccg ccgcggctct
    12661 gacccgggag gcga
//
LOCUS       DAOMPG010000046        12518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2180_length_12518_cov_4.215117, whole genome shotgun sequence.
ACCESSION   DAOMPG010000046 DAOMPG010000000
VERSION     DAOMPG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2180_length_12518_cov_4.215117"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..426
                     /gene="yajC"
                     /locus_tag="PLS34_03970"
     CDS             103..426
                     /gene="yajC"
                     /locus_tag="PLS34_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRP86661.1"
                     /translation="MDFFISNAWAQAGGQPDPFMSFLPLILIFVIFYFLLIRPQTKRA
                     KEHRKMVSELKTGDEVVTGGGILGRITEAGEQFIKVEVADGVVLRIQRQTVIAILPKG
                     TFKSV"
     gene            452..2305
                     /gene="secD"
                     /locus_tag="PLS34_03975"
     CDS             452..2305
                     /gene="secD"
                     /locus_tag="PLS34_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRP86662.1"
                     /translation="MVLNRFPLWKNLLVVAVVLVGLVFALPNIYGDAPAVQVTAETGQ
                     ALDETALQRVTGILDARQISHGPAYLEDGRVTVRFPDVEEQLRALDLVREQLGRGYVA
                     ALTLAPRTPAFLRAVGLKPMSLGLDLRGGVHFMYEVDMEAAIGQTLDRFETDFRTMLR
                     DAGIRYKSVRRDGDAVRITLENEQDRSAAARVIRDADRQLATSERQVGTDFELVVRMT
                     EEQVRERQNFAIEQNTVTLRNRVNELGVSEPVVQRQGADHIVVQLPGIQDPAQAERVL
                     GATATLEFRLVDEVNSPFEARERGRAPLGSALYTDRNGQPILLRRDVIATGDQLTDAS
                     AGFNEGQPAVFVNLDARGARKMGETTRANLQKRMAVVFIEQKREVQVRDGVEREVTRT
                     EERVISVATIQGVFSNRFMITGLDPLEARDLALLLRAGSLAAPIFKVEERTIGPSLGQ
                     ENIERGMQAVVIGFVLVVLFMVVYYRVFGLIANLALLANLVLVIALLSMLQAALTLPG
                     IAGIVLTVGMAVDANVLIFERIREELRNGNTPQAAIHAGYEKAFSSIADANITTLIAA
                     VVLFTFGTGPIRGFAVTLSLGIITSMFTAIVGTRALVNALYGGRRVQSLPI"
     gene            2319..3257
                     /gene="secF"
                     /locus_tag="PLS34_03980"
     CDS             2319..3257
                     /gene="secF"
                     /locus_tag="PLS34_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRP86663.1"
                     /translation="MDFFYIESHIQFLKVRKVAYAISAVLVLVSLASLATRGLNFGIE
                     FRGGVVIEAGFPESVDLPPIRTALESAGIDRTVVQHFGSSREVTVQLMAEEGADPAGT
                     RDRVMDVLRTVDAGAELRRVEFVGPQVGRELAEQGGLAALFALLMILAYVSFRFQWKL
                     SLGAVAALAHDVIITLGFFSLVQMPFDLAVLAAVLAVIGYSLNDTIVIFDRIRENMLK
                     MRRKSVEDVMDSSLNQTLSRTIMTGVTTLLVLLALYFLGGEAIAGFSVALIVGVLIGT
                     YSSIYIASPVALALKVTSLDLIPPQPDKDDESGVMP"
     gene            complement(3283..5250)
                     /locus_tag="PLS34_03985"
     CDS             complement(3283..5250)
                     /locus_tag="PLS34_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRP86664.1"
                     /translation="MQEPLLQILIALGLAVGVVLAFQRLRLPPVLGYLLVGVLLGPNT
                     IGPIIDARQIQLLAELGIVFLLFTIGLNYSIPQINALRGLLLTLGTGQVVLTTLVVAA
                     IAWGLGLPPAAAFVVGAVFAQSSTTIISRQLSEQQEDQSRHGRLGTAMSVFQDVTAVP
                     FIVIIPVLGVAAGGTVLGSLGLAVAKAGLAFVLVFLVGRWLLRPLFMAVAGRRSAELF
                     TLTVLLVSLAAAWTTKSFGLSMAFGAFLAGMMLGETKFRHQVEATIRPFRDVLLGLFF
                     VSIGMLFDPRVLPDIWVYAVAGAAGLLAVKAVLVAVLARIAGVDWLTAWQTGLIVAVG
                     GEFGFALLALGLDAAVISPEVSQASLVAVLFSMIAGAFLIRFNRGIALRLAPSTGDRT
                     GPTKNYDDLRMPPSGSSSNHVIICGYGRIGQSLGHCLEEENIPYIAVDLDPLRVSDAR
                     LAGEPVFFGDATNRDVLNALGIDRARLLVISHGDIPASERILQLVRELREDLPIMVRT
                     RDESPLEMLRAAGATEVVPETHEASLMIASHALLLLDVPLSHVARRIQRQWSGRYRLL
                     RELIPGDSLLAEDEDKAERLRPLRLREGCGAVGKTLAELPLEDVIVTALARNGRREIN
                     PPEDTLLAADDVIVLFGRPQDLARAQQALLG"
     gene            complement(5349..6149)
                     /locus_tag="PLS34_03990"
     CDS             complement(5349..6149)
                     /locus_tag="PLS34_03990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRP86665.1"
                     /translation="MHPILNIGVRAARRAGDLIVRHINQLDAIRVDSKGRNDFVSEVD
                     RMAEQDIIRTVQRSYPDHAFLAEESGASGDAEYVWIIDPLDGTTNFLHGFPVFCVSIA
                     VMHRGRLEHGVIFDPLRNELFTASRGAGASVDGRKMRVSDTRLLEKSLLGTGYPYKPE
                     SDWLDPYMDMLKSAIRETSGVRRPGAAALDLAYVAAGRLDGFWEIGLNIWDIAAGALM
                     IQECGGIVTDLAGRDGWQQSGNVVAGNPKIHEALLKLIEPHLTQDLKR"
     gene            6262..7008
                     /locus_tag="PLS34_03995"
     CDS             6262..7008
                     /locus_tag="PLS34_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRP86666.1"
                     /translation="MSLPVRIVLVGTTHPGNIGAAARAMRTMGLDRLTLVAPREFPSD
                     EATARAAGADQVLADARVCATLPEALAECRFAVGASARLRGLSWSTLSPREVAPRLLA
                     ESRDGEAAVVFGRESSGLSNDELACCHALLHIPTDPGFSSLNLGMAVQVVAYELRLAM
                     LGEGAPEPAPERLDPLATIEDLERFYVHLERTLVDAGFLNPDNPRHLMLRLRRLFSRA
                     LLEEKEVRILRGILTALEPRRDGGPPDALR"
     gene            7052..8239
                     /locus_tag="PLS34_04000"
     CDS             7052..8239
                     /locus_tag="PLS34_04000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRP86667.1"
                     /translation="MRTPVYFDYAATTPVDERVVQCMVECMGRGGCYANPASSSHGPG
                     REARGRVEAARREVAALLGAGPTEIIWTSGATESNNLAIKGAARFHADRGRHIVTSRT
                     EHKAVLDTCRQLEREGWQVSYVSPGPGGVVTAAEIEPALRPDTVLVSVMFVNNEIGTI
                     NELAPIGRLCRERGVLFHVDAAQAAGKVAVDVDAIGADLLSVSAHKAYGPKGAGALYL
                     RGSPKSRLEPLIHGGGHEWGMRSGTLATHQIVGMGEAFRIAAAEQAEEAARLRMLRER
                     LWQGIAGLGGVHLNGDPERRVAGVLNVSFEGVEGESLLFALRELAVSSGSACTSASRE
                     ASYVLRALGRDDQLAQSSLRFSLGRFSTAEEVDFAIGAVREQVNRLRGLGPGGGERNR
                     EAM"
     gene            8236..8634
                     /locus_tag="PLS34_04005"
     CDS             8236..8634
                     /locus_tag="PLS34_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRP86668.1"
                     /translation="MSTQSVTLDYSPEVQHRFEAAPRHGRVLAARAERRTGGAGEIAR
                     GVRVEFEMRVRDGRIAECRFRAYGCPHVIAAASWVAEHAEGRSVDEVEWFEPRRLAEL
                     LEAPAYKLGSLLVVEDAYRACVAGRPAPGR"
     gene            8688..9011
                     /gene="iscA"
                     /locus_tag="PLS34_04010"
     CDS             8688..9011
                     /gene="iscA"
                     /locus_tag="PLS34_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HRP86669.1"
                     /translation="MAITLTESAATRVKDYLARRGSGAGIRLGVKRTGCSGWAYVVDY
                     ADKVDEQDVVFEDHEVRVFVSPEHLELIDGTEVDFVKQGLNEAFRFRNPNVKGECGCG
                     ESFSV"
     gene            9144..9575
                     /gene="ndk"
                     /locus_tag="PLS34_04015"
     CDS             9144..9575
                     /gene="ndk"
                     /locus_tag="PLS34_04015"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRP86670.1"
                     /translation="MAIERTLSIIKPDGVQKNLIGEIYRRFEAGGLRLVAARMVHLSP
                     ADAGAFYAVHRERPFYNDLVSYMTSGPVMVTVLEGEGAIAKHREIMGATDPAKADKGT
                     IRADFADSIEENVVHGSDGADTARQEIAFFFAGREICPRTR"
     gene            9583..10689
                     /gene="rlmN"
                     /locus_tag="PLS34_04020"
     CDS             9583..10689
                     /gene="rlmN"
                     /locus_tag="PLS34_04020"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRP86671.1"
                     /translation="MSDARVNLLGLSREGLEEFFAGLGEKPFRARQLMSWMYRLGVTD
                     FEAMTDMSKALRARLAEIAEIRLPEMRSLHHAADDTRKWTVSMDGGQAIETVFIPETA
                     RGTLCISSQVGCAMDCSFCATGKQGFNRNLSAAEILGQVWLANRELGYRPDGDRIITN
                     VVFMGMGEPLANYRNVLPAIRTMIDDYGFNISRRRVTVSTSGLVPQIDRLGEECNVAL
                     AVSLHAADDTLRDRLVPINRRHPIAELLEACWRYAERGTGRHITFEYVLLDGVNDRPE
                     DMQRLAKLLAHRPAKVNLIPFNPFPGTEYRRSPVDTVERCREFLLRKGIRTTIRRTRG
                     DDIDAACGQLAGQVNDRMRYRLGSKLVSVSARAG"
     gene            10689..11435
                     /gene="pilW"
                     /locus_tag="PLS34_04025"
     CDS             10689..11435
                     /gene="pilW"
                     /locus_tag="PLS34_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HRP86672.1"
                     /translation="MTYRTLILLILAGLATGCASTAPTGDRVSDQDAARYNVQLGMNY
                     LQRGDLEAAREKLERAVQQDPSLPAAHAALGILYERAGDMRRAGDHLRRATRLAPDDP
                     NMVNNYGGFLCRQGERREGIRHFDLAAANPFYRTPEAALTNAGVCARGIPDPEAAENY
                     FRRALDANRNYADALLQLADLSLETQRPLQARAFLQRYEAVSSASSYSLALGHRIETA
                     AGDRQAASEYASRLRREFPDSREARGLNRD"
     gene            11428..>11723
                     /locus_tag="PLS34_04030"
     CDS             11428..>11723
                     /locus_tag="PLS34_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP86673.1"
                     /translation="MTDVRQAPFDAAPQAPLSPGMQLRAARERAGWTAERMAAELCLP
                     LDRLRALEEDDHASFGGVVFVRGYLRRAAALLRLPQDELIAAFEACCDTRRPAE"
     assembly_gap    11724..11733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11734..12324
                     /locus_tag="PLS34_04035"
     CDS             <11734..12324
                     /locus_tag="PLS34_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRP86674.1"
                     /translation="PPRRGAPGWLGPATGVAVVIAAAAGSWWMVNPGQDAPQLADSSN
                     DSSTAGLEFLAQPAPETSPAQIVVADHALTRDATPQVVDQATAPPPAEQPASTAPRVV
                     QAPEPPLPPPGTAELRFEFSEDCWLEVTDADDGRLAYRLYRAGDVARLRGKAPLQVFL
                     GNAEGVRLTVNGAPIAVRPAARRDGTARLTVGGGAG"
     gene            12340..>12518
                     /locus_tag="PLS34_04040"
     CDS             12340..>12518
                     /locus_tag="PLS34_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRP86675.1"
                     /translation="MSAAIQPIRGMNDILPDATPAWQWVEAAAREVFSAYGYRELRIP
                     IVERTELFSRSIGEVT"
BASE COUNT         1974 a         4265 c         4346 g         1923 t
ORIGIN      
        1 cggagcggcg aggaatgagg caagcggcgc gcatcgtggc ataatagccc gctgttttac
       61 cgccaccccc ttcggggcgg cgcaatccgg ggacgtactt cgatggattt cttcatctcc
      121 aacgcgtggg cccaggccgg cggccagccc gatcctttca tgagtttcct gccgctgatc
      181 ctgatcttcg tgatcttcta cttcctgctc atccggccgc agaccaagcg cgccaaggag
      241 caccggaaga tggtgtccga gctcaagacc ggcgatgagg tcgtgaccgg cggcggcatc
      301 ctcggccgca tcaccgaggc cggcgagcag ttcatcaagg tcgaagtcgc ggacggcgtg
      361 gtgctcagga tccagcgcca gaccgtcata gccatcctgc ccaagggcac cttcaagtcc
      421 gtctgaccgg cccgccgaca ggaattcatc cgtggttctc aaccgttttc ctctctggaa
      481 gaatctgctc gtcgttgcgg tggtgctggt cgggctggtg ttcgccctgc cgaacatcta
      541 cggcgatgcg ccggcggtgc aggtgaccgc cgagaccggc caggcgcttg acgagacggc
      601 tctgcaacgc gtcaccggca tcctcgacgc ccggcagatc agccacggcc cggcgtatct
      661 cgaggacggg cgcgtcaccg tgcgcttccc cgacgtcgag gagcagctgc gggcactgga
      721 cctggtgcgc gagcagctcg gccgcggcta cgtggccgcg ttgaccctgg cgccgcgcac
      781 cccggctttc ctgcgggccg tcgggctcaa gccgatgagc ctcggactcg acctgcgcgg
      841 cggcgtgcac ttcatgtacg aagtcgacat ggaagcggcg atcggccaga cgctggaccg
      901 cttcgagacc gacttccgca ccatgctgcg ggacgcaggc atccgctaca agtcggtgcg
      961 ccgcgacggc gacgccgtgc gcatcacgct ggaaaacgag caggaccgca gcgccgcggc
     1021 gcgggtcatt cgcgacgcag accgccagct cgccaccagc gagcgccagg tgggcaccga
     1081 cttcgagctg gtggtgcgca tgaccgagga gcaggtccgc gagcggcaga acttcgcgat
     1141 cgagcagaac accgtgacgc tgcgcaaccg tgtcaacgag ctcggcgtct ccgagccggt
     1201 ggtgcagcgg cagggtgccg accacatcgt cgtgcagctg cccggcatcc aggacccggc
     1261 gcaggccgag cgggtgctcg gcgccaccgc gaccctggag ttccgccttg tcgacgaggt
     1321 caacagtccc ttcgaggcgc gcgagcgcgg ccgcgccccg ctcggctcgg cgctgtacac
     1381 ggatcgcaac ggccagccca tcctgctgcg ccgcgacgtc atcgccaccg gcgaccagct
     1441 caccgacgcg tcggccggct tcaacgaggg ccagccggcg gtgttcgtca acctcgacgc
     1501 gcgcggcgcc cgcaagatgg gcgagaccac ccgcgccaac ctgcagaagc gcatggcggt
     1561 ggtgttcatc gagcagaagc gcgaggtcca ggtgcgcgac ggcgtggagc gcgaggtcac
     1621 gcgcaccgag gagcgcgtca tcagcgtggc caccatccag ggcgtgttct cgaaccgctt
     1681 catgatcacc ggcctcgatc cgctcgaggc gcgcgacctg gcgctgctgc tgcgcgcggg
     1741 ctccctcgcg gcgccgatct tcaaggtcga ggaacggacc atcggcccga gtctcggcca
     1801 ggaaaacatc gagcgcggca tgcaggcggt cgtcatcggc ttcgtgctgg tggtgctgtt
     1861 catggtcgtc tattaccggg tgttcggact gatcgccaac ctggcgctgc tggccaacct
     1921 ggtgctggtg atcgccctgc tgtcgatgct gcaggccgcg ctgaccctgc ccggcatcgc
     1981 cggtatcgtg ctgacggtcg gcatggcggt ggacgccaac gtgctgatct tcgagcgcat
     2041 ccgcgaggag ctgcgcaacg gcaatacgcc gcaggctgcg atccacgccg gctacgagaa
     2101 agctttttcg tcgatcgccg atgcgaacat caccaccctc attgccgcag tagtgttgtt
     2161 cactttcggc accggcccga tccgcggctt cgcggtgacg ctgtcgctcg gcatcatcac
     2221 ctccatgttc acggccatcg tcggcacgcg tgccctggta aacgccctgt atggcggccg
     2281 ccgcgtccag tcgctgccga tctgaggaga cggtcggaat ggatttcttc tacatcgaat
     2341 cgcacatcca gttcctgaag gtccggaagg tagcctatgc catttccgca gtgctggtcc
     2401 tggtgtcgct ggcctcgctg gcgacccgcg ggctgaattt cggcatcgag ttccgcggcg
     2461 gcgtggtgat cgaggccggc ttccccgagt ccgtggacct gccgccgatc cgcactgcgc
     2521 tcgagtctgc cggcatcgac cggacggtcg tgcagcactt cggcagctcg cgcgaggtca
     2581 ccgtgcagct gatggccgag gagggcgccg acccggcggg cacgcgtgat cgggtcatgg
     2641 acgtgctgcg caccgtggac gccggtgccg aactgcgccg cgtcgagttc gtcggcccgc
     2701 aggtgggccg ggagctggcc gagcagggcg gactggcggc cctgttcgcg ctgctgatga
     2761 tcctcgccta tgtgtccttc cgcttccagt ggaagttgtc gctcggcgcc gtcgcggcgc
     2821 tggcgcacga cgtcatcatc acgctgggct tcttctccct ggtccagatg ccgttcgacc
     2881 tggcggtgct ggcggcggtg ctggccgtga tcggctattc gctgaatgac accatcgtga
     2941 tcttcgaccg catacgcgag aacatgctga agatgcgacg caagtccgtc gaggacgtaa
     3001 tggacagctc gctcaaccag accctgtcgc gcaccatcat gaccggcgtg acgacgctgc
     3061 tggtgctgct ggcgctttat ttcctcggcg gcgaggcgat cgccggcttc tcggtcgcgc
     3121 tgatcgtcgg cgtgctgatc ggcacctact cgtccatcta cattgcgagc ccggtggcgc
     3181 tggcgctgaa ggtgaccagc ctggacctca tcccgccgca gcctgacaag gacgacgaat
     3241 cgggcgtgat gccctgacgg acgattgcgt gcaggacgcg cctcagccca ggagggcctg
     3301 ctgcgcgcgc gccaggtcct ggggcctgcc gaacaggaca atcacgtcgt ccgccgcgag
     3361 cagcgtgtcc tcgggcgggt tgatctcgcg ccgcccgttg cgcgccaggg cggtgacgat
     3421 cacgtcctcc agcggcagct cggccagcgt cttgccgacc gcgccgcagc cttcgcgcag
     3481 ccgcagcggc cgcagccgct ccgccttgtc ctcgtcctcg gccagcaggc tgtcgcccgg
     3541 aatgagctcc cgcagcaggc gatagcgacc ggaccactgc cgctggatcc gccgcgccac
     3601 gtgcgacagc ggcacgtcca gcagcagcag ggcatgcgat gcgatcatca ggctggcctc
     3661 gtgggtctcc ggcaccacct cggtggcgcc ggccgcgcgc agcatctcga ggggtgactc
     3721 gtcgcgggtt cgcaccatga tcggcaggtc ctcgcgcagt tcgcggacga gctgcaggat
     3781 ccgctcggag gccggaatgt cgccatggct gatgaccagc aggcgggccc ggtcgatgcc
     3841 gagcgcattg aggacgtcgc ggttcgtggc atcgccgaag aacaccggtt cgcccgccag
     3901 tcgcgcatcg gatacgcgca gtggatcgag gtccaccgcg atgtacggga tgttctcctc
     3961 ctcgaggcaa tggcccagcg actggccgat gcgcccgtag ccgcagatga tgacgtgatt
     4021 gctggagctg ccgctcggcg gcatgcgtag gtcgtcgtag ttcttcgtcg gtccggtgcg
     4081 atcgccggtg gacggcgcca ggcgcaaagc gatgccgcga ttgaagcgga tcaggaaggc
     4141 gccggcgatc atggagaaca gcaccgccac caggctggcc tgcgatactt ccggcgaaat
     4201 cacggcggca tccagcccca gggccagcag ggcgaagccg aattcaccac ccactgctac
     4261 gatgaggccc gtctgccatg cggtcagcca gtcgacacct gcgatccggg cgagcacggc
     4321 aaccagcacc gccttgaccg ccagcagccc tgcggcgccc gccacggcat acacccagat
     4381 gtccggcaac acccgcgggt cgaacagcat gccgatgctg acgaaaaaca agcccagcag
     4441 cacgtcgcgg aacggccgga tggtggcctc gacctggtgc cggaacttgg tttcgcccag
     4501 catcatgccg gcgaggaacg cgccgaaggc catggacagg ccgaagctct tggtggtcca
     4561 tgccgcggcc agcgacacca gcagcaccgt cagggtgaac agctccgccg aacgccgccc
     4621 tgcgaccgcc atgaacagcg ggcgcagcag ccagcggccg accaggaaca ccagcacgaa
     4681 ggccaggccg gccttggcca ccgccagtcc cagcgagccc agtaccgtac cgccggccgc
     4741 cacgccgagt accggaatga tgacgatgaa cggcaccgcg gtgacgtcct ggaacactga
     4801 cattgcggtg cccaggcggc cgtgccggct ctggtcctcc tgctgttcgc tgagttgcct
     4861 ggagatgatg gtcgtcgagg actgcgcgaa caccgcgccg accacgaagg cggccgcggg
     4921 cggcaggccc aggccccagg caattgcggc caccaccagc gtcgtcagta cgacctgccc
     4981 cgtgccgagc gtcagcagca gcccgcgcag cgcgttgatc tgcgggatgg agtagttcag
     5041 cccgatagtg aacagcagga acacgatgcc cagctcggcg aggagttgta tctgccgcgc
     5101 gtcgatgatc ggcccgatgg tgttcggtcc cagcagcacg ccgaccagca ggtagcccag
     5161 cacgggtggc agccgtaatc gctggaaagc aagcacgacg ccaacggcga ggccaagagc
     5221 gatcaggatt tgcagcagcg gttcttgcac gggacctcga taagcgttgc cggaccgttg
     5281 cagggattat cgacgatcgc ggcgagtgta gcacctcgga cccggtgcgc cctctcggca
     5341 cattccggtc agcgcttgag gtcctgcgtc aggtgcggct cgatgagctt gagcagcgcc
     5401 tcgtggatct tggggttgcc ggcgacgacg ttgccgcttt gctgccagcc gtcgcgcccg
     5461 gcgaggtccg tgacgatgcc gccgcactcc tggatcatca gcgcaccggc ggcgatgtcc
     5521 cagatgttca ggccgatctc ccagaagccg tccagccggc ccgcggcgac gtaagcgagg
     5581 tccagcgcgg ccgcgcccgg gcgccgcacc cccgaggtct cgcggatcgc ggacttcagc
     5641 atgtccatgt agggatccag ccagtcggac tcgggcttgt aggggtagcc ggtgccgagc
     5701 aaggacttct ccagcaggcg ggtgtcgctg acgcgcatct tgcgcccgtc gaccgacgca
     5761 cctgcgccgc ggctggccgt gaaaagctcg ttgcgcagcg ggtcgaagat cacgccgtgc
     5821 tccagccggc cgcgatgcat gaccgcgatg gagacgcaga acaccgggaa gccgtgcagg
     5881 aaattggtcg tgccgtcgag cgggtcgatg atccagacgt actcggcgtc gccgctggcg
     5941 ccgctttcct cggcgaggaa ggcatggtcg ggatagctgc gctgcaccgt gcggatgatg
     6001 tcctgctcgg ccatgcggtc gacttcgctg acgaagtcgt tgcggccctt ggagtcgacc
     6061 cggatggcgt ccagctggtt gatgtggcgg acgatgaggt cgccggcgcg ccgcgcggcg
     6121 cggacgccga tgttcaggat gggatgcatt gaatattctc ctccggcggg gcgggcggca
     6181 tgccctgtcg cgcgcgccag agccggtcac agccggttag aatagcaatt cgccccgctt
     6241 cctgcaggcc tttcgactcc catgagcctc cccgtccgca tcgtgctggt cggcaccacc
     6301 caccccggca acatcggcgc cgcggcgcgc gccatgcgca ccatgggcct tgaccggctg
     6361 acgctggtgg cgccgcgcga gttcccgagc gacgaggcca cggcgcgggc cgcaggcgcc
     6421 gaccaggtgc tggccgacgc ccgggtgtgc gccaccctgc cggaggcgct ggccgagtgc
     6481 cgcttcgcgg tcggcgccag cgcgcggctg cgcggcctgt cctggtcgac gttgagcccg
     6541 cgcgaagtgg cgccgcggct gctcgccgag tcccgcgacg gcgaggcggc ggtggtgttc
     6601 ggccgcgaga gcagcggcct gagcaacgac gagctggcct gctgccatgc cctgctgcac
     6661 atcccgaccg accccggctt cagttcgctc aacctgggca tggcggtgca ggtggtggcg
     6721 tacgagctgc gcctcgcgat gctgggcgag ggcgcacccg agccggcgcc ggaacggctc
     6781 gatccgctcg ccaccatcga ggacctggag cgcttctacg tccatctcga gcgcacgctg
     6841 gtggacgcag gtttcctcaa tcccgacaac ccgcgccacc tgatgctgcg gctgcggcgg
     6901 ctgttcagcc gggcacttct ggaggagaaa gaggtcagaa tcctgcgcgg catcctcact
     6961 gcgctggagc cgcgccggga tggcggcccg ccggatgcgt taaggtaatg cctatgttcc
     7021 cgttcgggcc acgccagcag aaatgaccga aatgcgtacg ccagtctatt tcgactacgc
     7081 cgccaccacg ccggtggacg agcgcgtcgt gcagtgcatg gtcgagtgca tgggccgcgg
     7141 cggctgctac gccaatccgg cctccagttc ccacggcccc ggccgcgagg cgcgcggccg
     7201 cgtcgaggcg gcgcgccgcg aggtggcggc gctgctgggc gccggcccga cggagatcat
     7261 ctggacctcg ggggcgaccg agtccaacaa cctcgccatc aagggcgcgg cgcgcttcca
     7321 tgccgaccgg gggcgtcaca tcgtcaccag ccgcaccgag cacaaggccg tgctggacac
     7381 ctgccggcag ctcgagaggg agggctggca ggtcagctac gtatcgccgg gacccggcgg
     7441 ggtggtgacc gccgccgaga tcgagccggc gctcaggccc gacacggtgc tggtgtcggt
     7501 gatgttcgtg aacaacgaga tcggcaccat caacgagctc gcccccatcg gccggctgtg
     7561 ccgggagcgc ggcgtgctgt ttcacgtcga tgccgcgcag gcggcgggca aggtggcggt
     7621 cgacgtggac gcgatcggcg ccgacctgct gtcggtgtca gcccacaagg cctacggccc
     7681 gaagggcgcc ggcgcgcttt atttgcgcgg cagtcccaag agccggctcg agccgctgat
     7741 ccacggcggc ggccacgagt ggggcatgcg ctccggcacg ctcgccaccc accagatcgt
     7801 cggcatgggc gaggcgttcc ggatcgccgc cgcggaacag gccgaggagg ccgcgcgcct
     7861 gcgcatgctg cgcgaacggt tgtggcaggg catcgccggg ctgggcggcg tgcatctcaa
     7921 cggcgatccc gagcggcgcg tcgccggcgt gctcaacgtg agcttcgagg gcgtggaggg
     7981 cgaaagcctg ctgttcgcgc tgcgcgagct ggcggtctcc agcggctcgg cctgcacttc
     8041 cgccagccgc gaggcgtcct acgtgctgcg ggcgctgggc cgcgacgacc agcttgcgca
     8101 gagctcgctg cgcttcagcc tcggccgctt cagcaccgcc gaggaagtgg acttcgccat
     8161 cggcgcggtc cgggaacagg tgaaccggct gcgcgggctc ggccccggcg gcggcgaacg
     8221 caacagggag gcgatgtgag cacgcagtcg gtgacactgg attattcacc cgaggtgcag
     8281 caccggttcg aagccgcgcc gcgccacggc cgggtgctgg ctgcccgggc cgagcggcgg
     8341 acgggcgggg ccggcgagat cgctcgcggc gtgcgggtcg agttcgagat gcgcgtgcgt
     8401 gacggccgga tcgcggagtg ccgcttccgc gcctatggct gcccgcacgt catcgccgcg
     8461 gcgtcctggg tggcggagca tgcggagggc cggtcggtcg acgaggtaga atggttcgag
     8521 ccgcgccgcc tggcggagct gctggaggcg ccggcctaca agctgggcag cctgctggtg
     8581 gtcgaggacg cctaccgcgc ctgtgtcgcg gggcggccgg cgcccgggcg ctgagaattc
     8641 gttgcgcccc gctggggcgc gggacaagac aggaggacga ggacaagatg gcgattaccc
     8701 tgaccgaatc ggcggccacg cgcgtcaagg attacctggc gcggcgcggc agcggcgcgg
     8761 gcatccgcct cggggtcaag cggaccggct gttcgggctg ggcctatgtc gttgactatg
     8821 ctgataaagt cgacgaacag gacgtggtat tcgaggatca cgaggtgcgg gtgttcgtct
     8881 cacccgagca cctcgagctg atcgacggca ccgaagtgga cttcgtcaag cagggcctca
     8941 acgaggcctt ccgtttccgc aaccccaacg tcaagggcga atgcggctgc ggcgagagct
     9001 tcagcgtctg attgtgcgcc cgggtcccgg ggcttaagat agtccgttcc gcgctccgtt
     9061 gagacggaga ttaggttgtg gccgcaggat gcggttttgc aacgaatttc agccggaatc
     9121 cagactgaat cgaagggaac ccaatggcga tcgaacgcac actctccatc atcaagcccg
     9181 acggcgtcca gaagaacctg atcggcgaaa tctaccggcg cttcgaggcc ggtggcctgc
     9241 gcctcgtcgc agcccggatg gtccacctgt ccccggccga cgccggcgca ttctacgcag
     9301 tgcatcgcga gcgtccgttc tacaacgacc tggtgtccta catgacctcg ggaccggtga
     9361 tggtgaccgt gctcgaaggc gagggcgcca ttgccaagca tcgcgagatc atgggtgcga
     9421 ccgatccggc caaggccgac aagggcacca tccgcgcgga cttcgccgac agcatcgagg
     9481 aaaacgtcgt gcacggctcg gatggggccg acacggcgcg ccaggaaatc gccttcttct
     9541 ttgccggcag ggaaatctgc ccgcggaccc gctgagccgc cggtgagcga cgcgcgcgtc
     9601 aacctgctgg gactgtcccg cgagggactg gaagagtttt tcgccggtct cggcgagaag
     9661 ccctttcgcg cgcgccagct gatgagctgg atgtaccggc tcggtgtcac cgacttcgag
     9721 gccatgaccg acatgtcgaa ggcgctgcgc gccaggctcg cggaaatcgc cgagattcgc
     9781 ctgccggaaa tgcgctcgct gcatcatgcc gccgacgaca cccgcaagtg gaccgtgtcc
     9841 atggacggcg gccaggcgat cgagacggtg ttcatcccgg agaccgcgcg cggcacgctg
     9901 tgtatctcca gccaggtggg ctgcgcgatg gattgctcgt tctgcgccac cggcaagcag
     9961 ggtttcaatc gcaacctctc cgcggccgag attctcggcc aggtgtggct ggccaaccgc
    10021 gagctcggtt accgccccga cggcgatcgc atcatcacca acgtggtgtt catgggcatg
    10081 ggcgagcctt tggccaacta tcgcaacgtg ctgccggcca tccgcaccat gatcgacgac
    10141 tacggcttca acatttcgcg ccggcgcgtc acggtgagca cctcgggcct cgtgccgcag
    10201 atcgaccggc tcggcgagga atgcaatgtg gcgcttgccg tgtcgctgca cgccgccgac
    10261 gacacgctgc gcgaccggct ggtgccgatc aaccgccgcc accccatcgc ggagctgctg
    10321 gaagcctgct ggcgctatgc cgagcgcggc accggccggc acatcacctt cgagtacgtg
    10381 ctgctcgacg gggtcaacga ccggcccgag gacatgcagc gtctcgccaa gctgcttgcg
    10441 caccgcccgg ccaaggtcaa cctgatcccg ttcaatccat ttcccggcac cgaataccgc
    10501 cgctcgcccg tcgatacggt cgagcgctgc cgcgagttcc tgctgcgcaa gggcatccgc
    10561 accaccatcc gtcgcacgcg cggcgacgac atcgacgccg cctgcggaca gttggccgga
    10621 caggtgaacg accggatgcg ataccggctg ggcagcaagc tggtcagcgt ttcggcgagg
    10681 gctggctgat gacgtaccgc acgttgatcc tgctgatcct ggccggcctg gcgacaggct
    10741 gcgcaagcac cgcgccgacg ggcgaccggg tctctgacca ggacgcggcg cgctacaacg
    10801 tgcagctcgg catgaactac ctgcagcgcg gcgacctcga ggcggcgcgc gagaagctcg
    10861 agcgcgccgt ccagcaggat ccgtccctgc ccgccgcgca tgcggcgctg ggcatcctct
    10921 acgagcgggc cggcgacatg cgccgcgccg gcgatcacct gcgccgcgcc acccgcctgg
    10981 cgccggacga ccccaacatg gtgaacaact acggcggctt tctctgccgc cagggcgagc
    11041 gccgcgaggg catccgccat ttcgacctgg cggcggcgaa cccgttctac cgcacaccgg
    11101 aggcggcgct caccaacgcg ggcgtctgcg cgcgcggcat ccccgatccg gaggcggcgg
    11161 agaactattt tcgccgggcg ctggatgcga accgcaacta cgccgatgcg ctgctgcaac
    11221 tcgccgacct gagcctggag acgcagcggc cgctgcaggc gcgtgccttc ctgcagcgct
    11281 acgaggccgt gtcgtcggcc tcgtcctaca gcctggccct gggtcatcgc atcgagactg
    11341 cggcgggcga ccgtcaggcg gcgagcgaat acgccagccg cctgcggcgg gaattccctg
    11401 attcgcgcga ggcgaggggg ctgaatcgtg actgacgtga gacaagcgcc gttcgacgcg
    11461 gcgccgcaag ccccgctttc accaggcatg cagctgcgtg cggcgcgcga gcgcgccggc
    11521 tggacggccg agagaatggc tgcagagctt tgcctgccgc tcgaccgctt gcgtgccctg
    11581 gaagaggacg accacgccag cttcggcggc gtggtgttcg tgcgcggcta cctgcgccgc
    11641 gccgcagcgc tgctcaggct gccgcaggac gagttgattg ccgccttcga ggcctgctgc
    11701 gatacccgcc gcccggcgga gatnnnnnnn nnnccgccac ggcgcggcgc gcccggatgg
    11761 ctcggtccgg cgacaggggt ggcggtagtg atcgcagcgg ccgccgggag ctggtggatg
    11821 gtcaatcccg gccaggacgc cccgcagttg gcggacagca gcaacgattc gtccacggcc
    11881 gggctggaat tcctggcgca gcccgcaccg gagacgtcgc cggcgcagat cgtagtggcc
    11941 gaccacgcgc tgacgcgcga tgcgacgccc caagtcgtgg accaggcgac cgcgccgccg
    12001 ccggccgagc agccggcgtc aaccgcgccg cgcgtggtgc aggcaccgga gccgccgctg
    12061 ccgccaccgg gcacggcgga actgcgtttc gagttcagcg aggattgctg gctggaggtc
    12121 acggacgccg atgacggccg gctcgcctat cgtctctacc gcgccggcga cgtcgccagg
    12181 ctgcgcggca aggcgccgct gcaggtattt ctcggcaacg ccgaaggtgt gcggctgacc
    12241 gtgaacggcg caccgatcgc ggtgcggccg gcggcgcgcc gcgacggcac cgcgcgactg
    12301 acggtcggcg gcggcgccgg ctgatcacgg gattcgacga tgtcggcggc catccagccc
    12361 atcaggggca tgaacgacat cctgccggat gcaacgcctg cgtggcagtg ggtggaggcg
    12421 gcggcacgag aagtgttttc cgcctacggt tatcgtgagc tgcgcatccc gatcgtggag
    12481 cgcaccgagc tgttcagccg ctccatcggc gaagtcac
//
LOCUS       DAOMPG010000047        12390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2201_length_12390_cov_3.847507, whole genome shotgun sequence.
ACCESSION   DAOMPG010000047 DAOMPG010000000
VERSION     DAOMPG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_12390_cov_3.847507"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLS34_04045"
     CDS             <1..529
                     /locus_tag="PLS34_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRP86676.1"
                     /translation="AERGLADADAAFISGGSAGGYTVLSALAFTDAFRAGASYYGVAD
                     LEGLFDTTHKFESRYDHRLLGVHGDPATRQRIAERSPLRYAHRIRCPVIFFQGGEDRV
                     VPPEQSRQMHAALRTAGIPTAYLEFPDERHGFRRAENIIAALEAELAFFCRVLGVNPA
                     GAGPERAFDNDAALH"
     gene            complement(593..1279)
                     /locus_tag="PLS34_04050"
     CDS             complement(593..1279)
                     /locus_tag="PLS34_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HRP86677.1"
                     /translation="MSLELETVERETAPAPAASVIWLHGLGADGYDFDPIVPALRLPG
                     GPEVRYVFPHAPIRPVTINGGAEMRAWYDIVALQRGAYEDEAGIRESASAIAALIRRE
                     TERGIPAHRVVLAGFSQGGAVALHAGLRYPERLAGIIGLSTYLPLRAAAGEFHAANRQ
                     TPVFMAHGSLDPMVPAVLGEESAAFLVESGYEVEFKTYTMPHSVCPEEVEDIRRWMGR
                     TLRGPLPPLS"
     gene            1412..1864
                     /locus_tag="PLS34_04055"
     CDS             1412..1864
                     /locus_tag="PLS34_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HRP86678.1"
                     /translation="MPSVNAQQNERREAILALLRQHRVTRQSDLVALLADRGYVATQS
                     SVSRDLRDLGVAKVGERYLAPQNVGGPATDFGALSGFLSGWSTAGPHLTVIRTSVGAA
                     QSVAVAVDRAGWNEVVGTISGDDTIFVATASARGQQALLARLNKIFPR"
     gene            1873..3084
                     /gene="argG"
                     /locus_tag="PLS34_04060"
     CDS             1873..3084
                     /gene="argG"
                     /locus_tag="PLS34_04060"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRP86679.1"
                     /translation="MNASPKTGDNPVLLAFSGGLDTSFCVAWLQETLGRPVITVTVDT
                     GGEVDAAALEQRALALGAARHITVDARREFFDTVIRYLVFGNVRRGQLYPLCVGAERG
                     LQARMVAEQAAALGSDTVAHGSTAAGNDQVRFEVALRTLRPGLEVLAPVRDHDWQRAA
                     QVAWLQERGLPVPPGGGAYSINRGLWGTTIGGKETLDSRESIPESAWVVTAGAFERPR
                     APERHRIGFERGVPVSLDGEALDPVALIERLEGLAGPFGIGRGIHLGETILGSKGRVA
                     FEAPAAETLLTAHRELEKLTLTGTQQKIKEPLALAYGDLVHEGQHLEPAARDIEALLA
                     SSQARVTGEVHLLFRPGVLFVEGVTSPHSLLAATRGKYGESAGEWSAADALGLARMKS
                     LAGMLHTRAGG"
     gene            3087..4148
                     /locus_tag="PLS34_04065"
     CDS             3087..4148
                     /locus_tag="PLS34_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86680.1"
                     /translation="MRPLIVDKIASVAQSCRLGREVRIAPEIPCEEGVVIAVEVLSNK
                     STYNTLELVSGRMAQVKRGDIVVGALGHRHALFGYSGHIPEKLAPGDKINLLNLGGVL
                     GICDSVNRDRGQPFECRVLGTVLHFPYLGERIGTPALVGSLAQPTDPVVDTRGIPAIA
                     LAGTCMDAGKTAAAAAIISRFSHYGYRVAAFKATGVSLRRDVLAMEDAGASATSIFTD
                     FGVVTTGPKNGPRLARTMLTRMADTRPDVIVFELGDGIMGAYGVEAILRDEQVRQALS
                     AVVLCANDPVGAFGGVHLLRERFGIEPALVTGRSTDNEVGVSIIREQMGVPAFNALTQ
                     EAELGEHLRGVLFGPEAGA"
     gene            4145..5203
                     /gene="argC"
                     /locus_tag="PLS34_04070"
     CDS             4145..5203
                     /gene="argC"
                     /locus_tag="PLS34_04070"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRP86681.1"
                     /translation="MSRRHGVAVLGGTGYVAGEFLRLLAGHPSLKLVAVSSESRAGQA
                     VAATFPNLALAWPDTRFCDEAGLEAAVRDGRVRALFSAAPHGAAAARIDALLHTAAAA
                     GAPLTIVDASADFRFRDAAAYQRVYGHPHPCPERLAEFASALPEHLDGCPAPHVGHPG
                     CFVTSVLLGIVPLLAAGLAEPEFFVSAVTGSTGVGRKLGETTHHPERHANMFAYQPLV
                     HRHRPEIEQLAAAAAAPCKVHFVPHSGAWARGIHATIHGRLAAGADGPALRAALDAAY
                     AGAPFVEVLDAPPRIKDVAGSNRARLAVAADGESFVVMSVIDNLVKGAAGGALQWMNR
                     LLDLPEDAGLRVPGPAWT"
     gene            5295..6353
                     /locus_tag="PLS34_04075"
     CDS             5295..6353
                     /locus_tag="PLS34_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP86682.1"
                     /translation="MADGRRVLDMYGGHAVAALGYGHPRFVAALQEQVKRLTFQSNLL
                     PLELRARACEALARFAPPGLDRVFLVNSGAEANENALKIAFGKPGRSRVLAVEGSFHG
                     RTAAAAAVTWGSEKWYGFPHKPFEVSFVSPHDPAALEHKLDEQVAALIVEPVQGVAGA
                     VDLPQAFLAAARQATRKAGALLIFDEVQCGMGRTGLPFAADYFGVTPDIITTAKGLAG
                     GFPAGAVITTAEIAAGLKIGELGTTFGGGPMACAAIETVLAVIREEGLLERVRTLSEQ
                     IRATCRVGPVTAIQGAGFLLGLVCSRPAREVQAELLARDILAGTSADPRVLRLLPPFI
                     LEPEHVARLAAALAEIAP"
     gene            6350..7360
                     /locus_tag="PLS34_04080"
     CDS             6350..7360
                     /locus_tag="PLS34_04080"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HRP86683.1"
                     /translation="MKRFIDLAEHAPEEVAELLELAERLDRHPEPQALTGKVLGMLFF
                     NPSLRTVASFQAAMGRLGGSSFVITPGAGTWKLETRNGIVMDGDAAEHVREAVPVLAS
                     YADVLGIRAFAGGIDLEADLEERKFMEMASVCPAPLVNLESEVNHPCQALADWKTMND
                     LGVPANGGRFVLSWANHPNALPLAVPSAVVHMAAMRGMDVTVLRPEGYALPAPIMQRA
                     RAAAAAYGGSLRETDDRAEALQGANVIYAKSWASTAHYGRPAEDRALRRELEHWMVRE
                     SWFADAAAEAIFMHCLPVRRNVVVADEVLDGPRSRVLPQARNRMTVQMAILHRMLKGA
                     GQ"
     gene            7366..8313
                     /gene="argB"
                     /locus_tag="PLS34_04085"
     CDS             7366..8313
                     /gene="argB"
                     /locus_tag="PLS34_04085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRP86684.1"
                     /translation="MLSSRGERAIAVTALKAAVPYIRMFKRRVFVVKAGGAVFGDEES
                     TRELVEQLGILHEVGIHVVLVHGGGPQSTELAAALDVPSQFVEGRRVTDERSLQVATM
                     VLNGIINTRVLAICRDIGLPAIGMSGVDAGLIQAHRRPPVALAGAQGGTVDYGFVGDI
                     DGVDSGVLRRQLDAGFLPIISPLSADGDGTLLNINADTVAAAIAVALQAEKLILVTGA
                     PGILEDHTDPGSLVSYLDLADLERMRAEGRLDAGMKPKADAIRFAVSGGVPRVHVISW
                     KVPDSLLLEVFTNEGTGTLVVQDTRSLSPEEQAAGEDDA"
     gene            8313..9527
                     /gene="argH"
                     /locus_tag="PLS34_04090"
     CDS             8313..9527
                     /gene="argH"
                     /locus_tag="PLS34_04090"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRP86685.1"
                     /translation="MSRMWDKGDPLDGRVLAYTAGEDHLLDNRLVPYDVRASIAHARM
                     LNARGLLSAEHLAAIEKGLQELAAAHARGDWRIGLDEEDVHNALEHRLSLVSPEAAAR
                     MHLGRSRNDQVLAALRLYLKDAARTLHDGALHVAAALESLGEREKDTPLPGYTHLQPA
                     MPSSVRLWAEGFAAELRDDAEGLLAASRADRFPLGSAAGYGAPGLPLDREHTRESLGF
                     AAIHEPVTAVQLSRGKAESQLVFEIHLLMQDLGRLAADLILFCSQEFSYVALPNSMTT
                     GSSIMPQKRNPDVFELVRGRAATTLGCLAETLAVPAKLTSGYHRDLQLLKAPLFRAID
                     LAEATLGIMAHALPEVNFIRENIRMEQQLYATEDAYRLVTEEGLSFRDAYRRIGEKLR
                     GLRDRTTGKDET"
     gene            9524..9955
                     /locus_tag="PLS34_04095"
     CDS             9524..9955
                     /locus_tag="PLS34_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRP86686.1"
                     /translation="MSIWHRRPSVEDLNKLSKNTLLEQLEIVFTEVGDDYLVATMPVD
                     HRTHQPFGLLHGGASAVLAESLGSSAANCCLDMTAEYAVGLEINANHLRSVRSGKVTG
                     TTRALHIGRRTQVWETRIEDDAGRLACVSRLTMAVVPRAAS"
     gene            9952..10194
                     /locus_tag="PLS34_04100"
     CDS             9952..10194
                     /locus_tag="PLS34_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86687.1"
                     /translation="MSRPARAWPLALLLLSGCGMYGDLYLEEEAPRLPEITEAPPIAV
                     DQPATGDASNGVNTTTPSEDEEREDRERGDNGAITS"
     gene            10191..11453
                     /gene="lysA"
                     /locus_tag="PLS34_04105"
     CDS             10191..11453
                     /gene="lysA"
                     /locus_tag="PLS34_04105"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRP86688.1"
                     /translation="MKPAFAYRDGRLCAEDVPLEQVAAKFGTPCYVYSRAALEARFDA
                     YLDAFAGHPARVCYAVKANANLAVLGVLAARGAGFDIVSGGELARVLAAGGAARHVVF
                     SGACKLDEELRYALEQEIACFNVESAPELERLSALAAEAGREARVSIRVNPDVEAGGH
                     RHISTGKREHKFGLAMEDAGALIERARALPGIRLTGVACHIGSQMMDLGPLAAALEQL
                     AAFVRGLQADGVVLEHVDAGGGLGIDHAGKRAPTPAAYAEVVIAALGGLGLEIVLEPG
                     RSIAGPAGLLLTRVAYLKQGHGRKFALVDAAMNDLLRPALYDAWHDILPATAGGTPDP
                     EPVDVVGPVCETGDFLGRDRRLAVRAGELLAVMDGGAYGFAMSSNYNARPRACEVMVS
                     GADAHLVRQRETVEDLWRGESLFEFAAR"
     gene            11450..12253
                     /locus_tag="PLS34_04110"
                     /pseudo
     CDS             11450..12253
                     /locus_tag="PLS34_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
BASE COUNT         1764 a         4489 c         4383 g         1754 t
ORIGIN      
        1 ggccgagcgg ggcctcgcag acgccgatgc ggccttcatc agcggtggga gcgcaggcgg
       61 ctacaccgtg ctctcggcgc tggccttcac cgatgccttc cgggcgggtg cgagctacta
      121 cggcgtggcc gatctcgagg gcctgttcga caccacccac aagttcgagt cgcgttacga
      181 ccaccggctg ctgggcgtgc acggggaccc cgccacccga cagcgcatcg ccgagcgctc
      241 gccgctgcgc tacgcgcacc gcatccgctg cccggtgatc ttcttccagg gcggcgagga
      301 ccgcgtggtg ccgcccgagc agagccgcca gatgcatgcc gccctgcgca cagccggcat
      361 cccgacggcc tatctcgagt ttcccgacga gcgccacggc ttccggcgcg ccgagaacat
      421 catcgcggcg ctcgaggcgg agctggcctt cttctgcagg gtgctcggcg tgaatcctgc
      481 cggtgcgggg ccggagcggg cgttcgacaa cgacgcggcg ctgcactgac gaccgcgggg
      541 tgcccatggt ggcgcgcccg cgcggtggcg gcgcgacaag tgtggacagg ccctaggaca
      601 gtggcggcag cggcccgcgc agcgtgcggc ccatccagcg gcggatgtcc tcgacttcct
      661 ccgggcacac cgagtgcggc atcgtgtagg tcttgaactc cacctcgtag ccggactcga
      721 ccaggaaggc cgcgctttcc tcgcccagca cggccggcac catgggatcg aggctgccgt
      781 gcgccatgaa gaccggcgtc tgccggttgg cggcgtgaaa ctcgccggcg gcggcgcgca
      841 gcggcagata ggtcgacagg ccgatgatcc cggcgagccg ctccgggtag cgcagcccgg
      901 catgcagcgc cacggcgccg ccctgcgaaa agccggccag caccacgcgg tgggccggaa
      961 tgccgcgttc ggtctcgcgc cggatcagcg cggcgatcgc cgaggcgctc tcgcggatgc
     1021 ccgcctcgtc ctcgtaagcg ccgcgctgca gggcgacgat gtcgtaccag gcgcgcatct
     1081 cggcgccacc gttgatggtc accggccgga tcggcgcatg cggaaacacg tagcggacct
     1141 ccgggccgcc gggcaggcgc aacgcgggca cgatcgggtc gaagtcgtag ccgtcggcgc
     1201 cgagcccgtg cagccagatc acgctggcgg ccggcgcggg cgcggtttcg cgttcgacag
     1261 tttccagttc gaggctcatc cggcggcaat ctccacgggg ggtggccggc agtgtacagc
     1321 gcccccggtg gcgcgacgag acttgacact ctatgcgcat tactgcaaat taatgcgcat
     1381 ctttaaccac gcctgcccgc cccacacccg catgccctca gtcaacgccc agcagaacga
     1441 gcgccgggag gccattcttg cgctcctgcg ccagcaccgc gtcacccgcc agtcggatct
     1501 cgtggcgctg ctggccgacc gcggctacgt cgccacccag tccagcgtca gccgcgacct
     1561 gcgcgatctc ggcgtggcca aggtcggcga gcgctacctg gcaccgcaga acgtcggcgg
     1621 gccggccacc gacttcggcg ccctcagcgg cttcctgtcc ggctggtcca cggccgggcc
     1681 gcacctgacc gtcatccgca cctcggtggg cgccgcccag agcgtggcgg tcgcggtgga
     1741 ccgcgccggc tggaacgaag tcgtgggcac gatctccgga gacgatacaa ttttcgtcgc
     1801 caccgcctcc gcccgcggcc agcaggccct gctggcccgc ctcaacaaga tcttcccgag
     1861 gtaaagctgc ccatgaacgc ctctcccaag acgggtgaca accccgtcct cctcgccttc
     1921 tccggcgggc tcgacacgtc gttctgcgtg gcgtggctgc aggagactct cggccggccg
     1981 gtgatcaccg tgacggtgga caccggcggc gaggtcgacg ccgcggcgct cgagcagcgc
     2041 gccctggcgc tcggcgcggc gcgccacatc accgtcgacg cgcgccgcga gttcttcgac
     2101 acggtgatcc gttacctggt gttcggcaac gtgcgccgcg gccagctcta cccgctgtgc
     2161 gtcggcgcgg agcgcggcct gcaggccagg atggtcgccg agcaggcggc cgcactcggc
     2221 agcgacaccg tcgcgcacgg cagcaccgcg gccggcaacg accaggtgcg cttcgaagtg
     2281 gcgctgcgca ccctgcgccc gggcctggag gtgctggcgc cggtgcgcga ccacgactgg
     2341 cagcgtgccg cccaggtcgc ctggctgcag gagcgcggcc tgcccgtgcc gccgggcggc
     2401 ggcgcctatt ccatcaaccg cggcctgtgg ggcacgacca tcggcggcaa ggagacgctg
     2461 gactcgcgcg agtccatccc ggaaagcgcc tgggtggtca ccgccggcgc cttcgagcga
     2521 ccgcgcgccc ccgagcgcca tcgcatcggc ttcgagcgcg gcgtgccggt ttcgctggac
     2581 ggcgaggcgc tcgatccggt cgcactgatc gagcggctcg agggcctggc cggccccttc
     2641 ggcatcggcc gcggcattca ccttggcgag accatcctgg gctcgaaggg gcgcgtggct
     2701 ttcgaggcgc ccgccgcgga aaccctgctg acggcgcatc gcgagctcga gaagctcacg
     2761 ctgaccggca cccagcagaa gatcaaggaa ccgctggcgc tggcctacgg cgacctggtg
     2821 cacgagggcc agcacctcga gcccgcggcg cgcgacatcg aggcgctgct ggcgtcctcg
     2881 caggcacggg tgaccggcga ggtacacctg ctgttccggc cgggcgtgct gttcgtcgag
     2941 ggcgtcacct cgccgcactc gctgctcgcc gccacgcgcg gcaagtacgg cgagtcggcg
     3001 ggcgagtgga gcgcggcgga cgcgcttggc ctggcgcgca tgaagtcgct ggcgggcatg
     3061 ctgcacaccc gggcgggagg ttgaacatgc gcccgctgat cgtcgacaag atcgcctcgg
     3121 tcgcgcaaag ctgccggctc ggccgcgagg tgcgcatcgc ccccgaaatc ccctgcgagg
     3181 aaggcgtggt catcgccgtc gaggtgctgt ccaacaagtc cacctacaac accctggagc
     3241 tggtctcggg ccgcatggcg caggtcaagc gcggcgacat cgtggtgggc gcgctgggtc
     3301 accgccatgc tctgttcggc tactcgggcc acattccgga gaagctcgcg ccgggcgaca
     3361 agatcaacct gctgaacctc ggcggcgtgc tgggcatctg cgactcggtg aaccgcgatc
     3421 gcggccagcc cttcgagtgt cgcgtgctcg gcaccgtgct gcatttcccg tacctcggcg
     3481 agcgcatcgg cacaccggcg ctggtcggca gcctggcgca gccgaccgat ccggtggtcg
     3541 acacgcgcgg catcccggcg atcgcgctgg ccggcacctg catggacgcc ggcaagaccg
     3601 ccgccgccgc ggccatcatc agccgcttct cgcactacgg ctaccgcgtc gccgcgttca
     3661 aggccaccgg cgtgtcgctg cggcgcgacg tgctggcgat ggaggacgcc ggcgccagcg
     3721 cgacctcgat cttcaccgat ttcggcgtcg tgaccaccgg cccgaagaac ggcccgcgcc
     3781 tggcccgcac catgctgacg cgcatggccg acacccgccc cgacgtgatc gtcttcgaac
     3841 tgggtgacgg catcatgggc gcctatggcg tcgaggccat cctgcgcgac gagcaggtgc
     3901 gccaggcgct gtcggcggtg gtgctgtgcg ccaacgaccc ggtcggcgcc ttcggcggcg
     3961 tgcacctctt gcgcgagcgc ttcggcatcg agcccgcgct ggtcacgggt cgttccaccg
     4021 acaacgaagt cggcgtctcc atcatccgcg aacagatggg cgtgccggct ttcaacgcgc
     4081 ttacccagga ggccgagctc ggcgagcacc tgcgcggcgt gctgttcggc cccgaggccg
     4141 gcgcatgagc cggcgccacg gcgtcgccgt gctgggcggc accggctacg tggccgggga
     4201 attcctgcgc ctgctggcgg gacatccatc gctgaagctc gtcgccgtgt cctccgagag
     4261 ccgcgccggg caagccgtgg ccgcgacctt cccgaacctc gccctggcct ggccggacac
     4321 ccgcttctgc gacgaagccg ggctcgaggc ggcggtacgg gacggccgcg tccgcgcgct
     4381 gttctcggcc gcgccgcacg gtgcggccgc ggcgcgcatc gatgcgctgc tgcacactgc
     4441 cgccgcggcc ggcgcccctt tgacgatagt ggatgcctcg gccgacttcc gctttcgcga
     4501 cgccgccgcc taccagcggg tctacggcca cccccatccc tgcccggaac ggctcgccga
     4561 gttcgccagc gcgctgcccg agcacctcga cggctgcccg gcgccgcacg tcggccaccc
     4621 gggctgcttc gtcacctccg tgttgcttgg catcgtgccg ctgctggcgg ccgggctggc
     4681 cgagccggaa tttttcgtct ccgccgtgac cggcagcacc ggcgtcggcc gcaagctggg
     4741 cgagaccacc catcaccccg aacgccacgc caacatgttc gcctaccagc cgctggtgca
     4801 ccggcatcgc ccggagatcg agcagctggc ggccgcggcc gcggcgccct gcaaggtgca
     4861 tttcgtgccg cactccggcg cctgggcgcg cggcatccat gccaccatcc acggccggct
     4921 cgccgccggg gcggatggcc cggcgttgcg cgccgcgctc gatgccgcct atgcgggcgc
     4981 gccgttcgtc gaggtcctgg atgcgccgcc gcgcatcaag gacgtcgccg gctccaaccg
     5041 cgcgcggctc gcggtcgccg ccgacggcga gtctttcgtc gtcatgtcgg tgatcgacaa
     5101 tctcgtcaag ggcgccgccg gcggcgcgct gcaatggatg aaccgcctgc tcgacctccc
     5161 ggaggacgcc gggctccgcg tgccggggcc ggcctggaca tgacgccaca agccactccc
     5221 gcaccgcctg gacgctgccg gtctacgccc agctcgacct gcatcccgtc ggcggggaag
     5281 gcgcctggct gctcatggcc gacggccgcc gggtactcga catgtacggc ggccacgcgg
     5341 tcgcggcgct cggctacggc catccgcgct tcgtcgccgc gttgcaggag caggtcaagc
     5401 ggctgacctt ccagagcaac ctgctgccgc ttgagctgcg ggcgcgtgcc tgcgaggcgc
     5461 tggcgcgctt cgcgccgcct ggcctcgatc gagtgttcct ggtcaacagc ggcgccgagg
     5521 ccaacgaaaa cgccctgaag atcgccttcg gcaagccggg ccgcagccgc gtgctggccg
     5581 tcgagggcag ctttcacggc cgcaccgcgg cggcggctgc cgtgacctgg ggcagcgaga
     5641 agtggtacgg cttcccgcac aagcccttcg aggtcagctt cgtgtcgccg cacgatccgg
     5701 cggcgctcga gcacaagctc gacgagcagg tggcggccct gatcgtcgag ccggtgcagg
     5761 gggtcgccgg cgcggtcgac ctgccgcagg ccttcctggc cgccgcccgc caggcgaccc
     5821 gcaaggccgg cgccctgctg atcttcgacg aggtccagtg cggcatgggc cgcaccggcc
     5881 tgccgttcgc cgccgactac ttcggcgtca cgcccgacat catcaccacc gccaagggcc
     5941 tggcgggcgg cttcccggcc ggcgccgtga tcaccaccgc cgagatcgcc gccggcctga
     6001 aaatcggcga gctgggtacg actttcggcg gcgggccgat ggcctgcgcc gccatcgaga
     6061 cggtgctcgc ggtgattcgc gaggagggcc tgctggagcg cgtgcggacc ctctccgagc
     6121 agatccgcgc aacctgcagg gtcggcccgg tcaccgccat ccagggcgcc ggcttcctgc
     6181 tcggcctggt ctgcagccgg ccggcgcggg aagtccaggc cgagctgctg gcgcgcgaca
     6241 tcctcgccgg caccagcgcc gatccgcgcg tgctgcggct gctgccaccg tttatcctcg
     6301 aaccggaaca cgttgcccgc ctcgcggcgg cccttgcgga gatcgctcca tgaaacgctt
     6361 catcgacctg gcagaacacg cccccgagga agtcgccgag ctgctcgagc tcgccgaacg
     6421 actcgatcgt cacccggaac cgcaggcgct caccggcaag gtgctcggca tgctgttctt
     6481 caatccctcg ctgcgcaccg tcgcgtcctt ccaggccgcg atgggccggc tcggcggctc
     6541 ttctttcgtc atcacgccgg gcgccggcac ctggaagctc gagacgcgca acggcatcgt
     6601 catggacggc gacgcggcgg agcacgtgcg cgaggccgtg ccggtgctgg cgagctacgc
     6661 cgacgtgctg ggcatccgcg cctttgccgg cggcatcgat ctcgaggccg atctcgagga
     6721 gcgcaagttc atggagatgg ccagcgtctg cccggcgccg ctggtcaacc tcgaatccga
     6781 ggtcaaccat ccctgccagg cactggcgga ctggaagacc atgaacgacc tcggcgtgcc
     6841 ggcgaacggc gggcgcttcg tgctgtcctg ggccaaccat ccgaacgcgc tgccgctggc
     6901 ggtgccctcg gcggtggtgc acatggccgc gatgcgcggc atggacgtca cggtgctgcg
     6961 accggagggc tatgcgctgc cggcgccgat catgcaacgg gcccgcgcgg ccgcggcggc
     7021 ctacggcggc tcgctgcgcg agaccgacga ccgtgccgag gcactgcagg gcgcaaacgt
     7081 gatctatgcc aagagctggg catcgaccgc gcattacggg cgcccggccg aggaccgcgc
     7141 cctgcgccgc gagctggagc actggatggt gcgcgagtcc tggttcgccg acgccgcggc
     7201 cgaggcgatt ttcatgcact gcctgccggt gcggcgcaac gtcgtggtcg ccgacgaggt
     7261 gctcgacgga ccgcgcagcc gggtgctgcc gcaggcgcgc aacaggatga ccgtgcagat
     7321 ggcgattctc caccgcatgc tgaaaggagc cggccagtga gcccgatgct gagttcgcgc
     7381 ggcgaacgcg ccattgcggt gacggccctg aaggccgcgg tgccctatat ccgcatgttc
     7441 aagcgccggg tgttcgtggt gaaagccggc ggcgcggtgt tcggcgacga ggagagcacg
     7501 cgcgaactgg tcgagcaact cggcatcctc cacgaggtgg gcattcacgt ggtgctggtg
     7561 cacggcggcg gcccgcagtc gaccgagctg gcggccgcgc tcgacgtgcc ctcgcagttc
     7621 gtggaaggcc ggcgcgtcac cgacgagcgc tcgctgcagg tcgccaccat ggtgctgaac
     7681 ggcatcatca acacccgcgt gctggcgatc tgccgcgaca tcggcctgcc ggccatcggc
     7741 atgagcggtg tcgacgccgg cctgatccag gcgcaccgcc gcccgccggt ggcgctcgcc
     7801 ggcgcgcagg gtggcacggt cgactacggc ttcgtcggcg acatcgacgg cgtcgacagc
     7861 ggcgtgctgc gccgccagct cgatgccggc tttctgccga tcatcagccc gctgtcggcc
     7921 gacggcgacg gcacgctgct gaacatcaac gcggacaccg tggccgcggc gatcgccgtg
     7981 gcgctgcagg ccgagaagct gatcctggtg accggcgcgc ccggcatcct cgaggaccac
     8041 accgatcccg gctcgctggt gtcctacctc gacctcgccg acctcgagcg catgcgtgcc
     8101 gagggacggc tggacgccgg catgaagccc aaggcggacg ccatccgctt cgcggtgagc
     8161 ggcggcgtgc cgcgcgtgca cgtgatctcc tggaaggtgc cggacagcct gctgctcgag
     8221 gtgttcacca acgagggcac cggcacgctg gtggtgcagg acacgcgcag cctgtcgccg
     8281 gaggagcagg ccgccggcga ggacgacgcc tgatgagccg catgtgggac aagggcgatc
     8341 cgctcgacgg ccgggtgctg gcctacaccg ccggcgagga tcacctgctc gacaaccgcc
     8401 tggtgcctta cgacgtgcgc gcctcgatcg cccatgcccg catgctgaat gcccgcgggc
     8461 tgttgtcggc cgagcacctc gccgccatag aaaagggcct gcaggaactc gccgcggcgc
     8521 acgcgcgcgg cgactggcgc atcggcctcg acgaggaaga cgtgcacaac gccctcgagc
     8581 accggctgtc gctggtctcg ccggaggccg ccgcgcgcat gcacctcggc cgctcgcgca
     8641 acgaccaggt gcttgccgcg ctgcgcctgt acctcaagga cgcggcgcgg acgctgcacg
     8701 acggtgcgct gcacgtcgcc gccgcgctgg agtcgctcgg ggagcgggag aaagacacgc
     8761 cgctgcccgg ctacacccac ctgcagccgg ccatgccgag ctcggtgcgc ctgtgggcgg
     8821 agggttttgc cgccgagctg cgcgacgacg ccgagggcct gctcgcggcc agccgcgccg
     8881 accgcttccc gctcggctcg gcggccggct acggcgcccc ggggctgccg ctggaccgcg
     8941 agcacacccg cgagagtctc ggcttcgccg ccatccacga gccggtgacc gcggtgcaat
     9001 tgtcgcgcgg caaggccgag tcccagctgg tgttcgagat tcacctgctg atgcaggacc
     9061 tcggccggct ggccgccgac ctgatcctgt tctgcagcca ggaattcagc tacgtcgccc
     9121 tgccgaactc gatgaccacc ggctcgtcga tcatgccgca gaagcgcaat cccgacgtgt
     9181 tcgaactggt gcgcggccgc gccgccacca cgctcggctg cctggcggag acactggcgg
     9241 tgccggccaa gctgacctcg ggctatcatc gcgacctgca gctgttgaag gcgccgctgt
     9301 tccgcgcgat cgacctcgcc gaggcgactc tcgggatcat ggcgcacgcg ctgcccgagg
     9361 tgaacttcat ccgcgagaac atccgcatgg aacagcagct ctacgccacc gaggacgcct
     9421 accgcctggt caccgaggaa ggcctgtcgt tccgcgacgc ctaccggcgc atcggcgaga
     9481 agctgcgggg gctgcgcgac cggacgacgg gcaaggacga gacatgagca tctggcaccg
     9541 gcggccctcg gtcgaggacc tgaacaagct gtcgaagaac acgctgctgg agcagctcga
     9601 gatcgtgttc accgaggtcg gcgacgatta cctggtggcc accatgccgg tggaccatcg
     9661 cacccaccag cccttcggcc tgctgcacgg cggcgcctcc gcggtgctgg ccgagtcatt
     9721 gggcagctcg gcggcgaatt gctgcctgga catgaccgcc gagtacgcgg tggggctcga
     9781 gatcaacgcc aatcacctgc ggtcggtgcg cagcggcaag gtcacgggca ccacccgcgc
     9841 gctgcacatc gggcggcgca cgcaggtgtg ggagacgcgc atcgaggacg acgccggcag
     9901 gctggcctgc gtgtcgcgcc tgaccatggc cgtggtgccg cgggccgcgt cgtgagtcgc
     9961 ccggcgcgcg cctggccgct ggccctgctg ctgctttcag gttgcggcat gtacggcgac
    10021 ctgtacctgg aagaagaggc gccgcgcctg cccgagatca ccgaggcgcc gccgattgcc
    10081 gtggaccagc cggcgacggg cgacgcctcg aacggcgtga acaccaccac gccgagcgag
    10141 gacgaggagc gcgaggaccg ggagcgcggg gacaacgggg caatcacctc gtgaagccgg
    10201 cctttgccta ccgcgacggg cggctgtgcg ccgaggacgt gccgctcgag caggtcgcag
    10261 cgaaattcgg cacgccgtgc tacgtctatt cgcgcgccgc gctcgaggcg cgcttcgacg
    10321 cttacctcga tgcttttgcg ggccacccgg cgcgcgtctg ctatgccgtc aaggcgaacg
    10381 ccaacctcgc ggtgctcggc gtgctggcgg cgcgcggcgc gggcttcgac atcgtctccg
    10441 gcggcgagct ggcgcgcgtg ctcgcggccg gcggcgccgc ccgccacgtg gtgttctcgg
    10501 gcgcctgcaa gctggatgaa gaactgcgtt atgcgctcga gcaggagatc gcctgcttca
    10561 acgtcgaatc cgccccggaa ctcgagcggc tgtcggcact cgccgccgag gcgggccggg
    10621 aggcgcgcgt gtccattcgc gtcaacccgg acgtcgaggc cggcggccac cgccacatct
    10681 ccaccggcaa gcgcgagcac aagttcgggc tggccatgga ggacgccggc gcgctgatcg
    10741 agcgtgcccg cgccctgccc ggcatccggc tgaccggcgt cgcctgtcac atcggctcgc
    10801 agatgatgga tctggggccg ctggccgcgg cgctcgagca gctcgccgcc ttcgtgcgcg
    10861 gcctgcaggc cgacggcgtc gtgctggagc acgtcgacgc cggtggcggg ctgggcatcg
    10921 atcacgccgg caagcgcgca cccacgccgg cggcgtacgc cgaggtggtg atcgcggcgc
    10981 tcggtgggct gggcctggaa atcgtgctcg agccgggccg ctccatcgcc gggccggcag
    11041 gcctgctgct gacccgggtc gcctatctca agcagggcca cgggcggaag ttcgcgctcg
    11101 tcgacgcggc gatgaacgac ctgctccggc cggcgctgta cgatgcctgg cacgacatcc
    11161 tgccggccac cgcgggcggc acgccggacc ccgagcccgt cgacgtggtc ggcccggtgt
    11221 gcgagacggg ggatttcctc ggccgcgacc gccggctggc ggtgcgcgcg ggcgaactgc
    11281 tggcggtgat ggacggcggc gcctatggtt tcgccatgag ttccaactac aacgcccggc
    11341 cgcgcgcctg cgaggtcatg gtgtccggcg cggatgcgca cctcgtccgc caacgggaga
    11401 ccgtcgagga tctctggcgt ggcgagtcgc tgttcgagtt cgcggcgcga tgagcgacaa
    11461 gccgctggtc ctggtggacg gctcctcgta cctgtatcgc gccttccacg cgatgccgcc
    11521 cttgaccaac agcaaggacg agccggtcgg cgcggtgctc ggggtggtca acatgctgct
    11581 caagctggcg gacgacaccc aggcggagca cttcgcggtg gtgttcgacg cgcgcggcaa
    11641 gactttccgc gacgagctgt tcgccgagta caaggccacc cggccgccga tgccggacga
    11701 gctctcggca caggtggcgc cgctgctcga ggtggtcgag gcgctcggcc tgccgatgct
    11761 gcaggtgccg ggcgtcgagg ccgacgacgt catcggcacg ctcgcccacc aggcctgcgc
    11821 ggcgggcatg aagacgctga tctcgaccgg cgacaaggac atggcgcagc tggtcaacgg
    11881 cgacgtcacg ctggtcaaca ccatgtccgg cacgacgctc gaccggcagg gcgtgatcga
    11941 caagttcggc gtgccgccgg agcgcatcgt cgactggctg gcgctggtcg gcgacacctc
    12001 cgacaacatt cccggcgtgc cgaagtgcgg ccccaagacg gccaccaagt ggctcgccga
    12061 gtacggcgac ctcgacaccc tcaaggcgcg cgccgacgaa gtgggcggca agatcggcga
    12121 gagcctgcgc gagaatctcg accggctgga gttgtcgcgc gagctggcca ccatccggct
    12181 ggacgtcgag ctggacctcg gcccggcgga cctggcgcgg cgcccgccgg acaaggcacg
    12241 gctgcgcgag ctgtacggca agctcgaatt caacacgctg ctgcgcaagc tcggcgacgg
    12301 cgaggacccg cccgaggagg cggcagactc agccgatccg cccaccagtc gatgaacttg
    12361 cggccctgag acgtcatccg gacgccgagg
//
LOCUS       DAOMPG010000048        11964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2311_length_11964_cov_5.109917, whole genome shotgun sequence.
ACCESSION   DAOMPG010000048 DAOMPG010000000
VERSION     DAOMPG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2311_length_11964_cov_5.109917"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLS34_04115"
     CDS             complement(<1..247)
                     /locus_tag="PLS34_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP86689.1"
                     /translation="MSKDPDERLVRACRNGDRAAFETLLARYERPVYNVAIRMLRHPE
                     DARDVAQTTFLKAFEKLEQFDFGHRFYSWIYRIAINES"
     gene            complement(291..593)
                     /locus_tag="PLS34_04120"
     CDS             complement(291..593)
                     /locus_tag="PLS34_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP86690.1"
                     /translation="MFDIQSRGEGRIVISGRLDASQARRAQEFIDGIENPREFDLARL
                     EYISSAGLGILLRTQKRVMSQGGALRLVNVGNHIRDVFRYSGFDKIFVIEPSPGAD"
     gene            complement(598..999)
                     /locus_tag="PLS34_04125"
     CDS             complement(598..999)
                     /locus_tag="PLS34_04125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP86691.1"
                     /translation="MERLFRRDFDSLAEIFALTEEFFAAAGIDPEQLYTVNLAVEELF
                     TNMVKFNPDGDEHISLQLQHADRAVRVRLCDRRVEPFDVTVPRLVDRNAPVEQRETGG
                     LGLYLVQQMVDTLDYVHRDGVTTVTFTKNLG"
     gene            complement(1068..2339)
                     /locus_tag="PLS34_04130"
     CDS             complement(1068..2339)
                     /locus_tag="PLS34_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP86692.1"
                     /translation="MSTGAARQLGSETLAGILEATVELGRPIEHLAILDEVAAAATRL
                     LDAERSSVFLLDPATDELVMTVVGDMPPIRVPNGRGIVGHCARERRLVNVPDCAADPR
                     FNAAVDRLSGFHTRCLLSLPLLHQDGRLFGVLQVLNKRGGTFDAHDELVGSILAAQSA
                     SALQRAQAVEERLAADHLHEEIRVAREIQMSGLPDVMPPLAGYDGAGLFLPTDQTGGD
                     LFDFVPVGTHGMFLLLGDATGHGIGPALSATQVRAMIRVALRLGADLDDIHTHVNNQL
                     LDDLPDDRFVTAFLGMLDARSHTLRFHAAGQAPLLHLHAAGGEAAWHDATTIPMGAMP
                     LAEPFPAKTIEFAPGDLFALLSDGVYEYEDAARRPFGEQRVADLLRQLQHRPMREAAR
                     ELHAALREFGGGAAQADDVTIVLLRRLPGME"
     gene            complement(2491..2811)
                     /locus_tag="PLS34_04135"
     CDS             complement(2491..2811)
                     /locus_tag="PLS34_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HRP86693.1"
                     /translation="MNDRIVADEAARTEIEARVFRKLVEHLRAHPEVQNLDMMNLAYF
                     CRNCLSKWYVGAAAEAGVVLDYEQAREIVYGMPYGEYKRRHQREATPEQLEKFAAAEQ
                     KATP"
     gene            complement(2846..5737)
                     /locus_tag="PLS34_04140"
     CDS             complement(2846..5737)
                     /locus_tag="PLS34_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRP86694.1"
                     /translation="MKLSVAMLAMLLTVACATPPAGAGGNAGAAVEAAGGDDKARVFD
                     LPWQERELANGLKVIVVKTDFPDIVSLQIPVQTGSRNEVEPGKTGFAHFFEHMMFRGT
                     ENVSPEEYNAALKLAGADTNAYTTDDYTNYHIDFTKADLDTVLALEADRFMNLSYGED
                     VFRTEALAVKGEYIKNSANPIRKIFEQVRATHYREHTYRHTTMGFFEDILEMPEQYEY
                     SRLFFDRWYRPEKSAVILVGDLEFEPTFALVEKHFGPWQRGSYQAEIPVEPPGEGPTS
                     VYVPVDMPTQPWIAVAFRGPGFDPRDPDTAAVQMIAQHYFSDNSDLYRKLVLERQVVD
                     QLFAYMPMNRDPGLFWVLARVVDPAATAEVRDEILATFARARTETLSPDKLQRIKDRM
                     RFGFLAGLDNSPAIGAALAGFVQYERSVETVDALYATFAALAPADLTAAADRHFRDAV
                     RTVGIIASGDSLAGLEAGGPSVDALAAAATTGEAAFRLVERHSDASPLVDVSYVFRAG
                     AMLDPPGKKGLAQLTAMMLTEAGSASRSIDQINDALYPMAAGFGAQVDKEMVRLSGTV
                     HRDNLAAWYGLTAEQLLAPGWREEDFRRLQTQLVNAILTDLVANNDEELGKEALYQFI
                     YGPEHPYGTLTLGRVEDVQALTLDDVQAFYAAHYHPANLTVGLAGGYPAEFVATLRRD
                     LARLPTVDPAAAPASPGSPAPVRGHQALILEKDTMAHAVSFGFPIELKRGDPDWVPLW
                     LATQWLGQHRSQNARLFQAIREQRGMNYGNYAYIEYFPRGMYLTQPDANLFRRQQIFQ
                     VWLRPLTGNNEAHFATRAALYELDRLIEQGLSEAEFEATRAFLDKWVAQLVSSQPALL
                     GYAIDSDWYGIPDFPAYVRQALAGLTLDDVNRVIRERLSTRDIKFVFVTPDAADLKRR
                     LLGNERSPMSYNSPKPEALLAEDAVIEVMPLGFGADSIEVVPADSVFRR"
     gene            5900..7720
                     /locus_tag="PLS34_04145"
     CDS             5900..7720
                     /locus_tag="PLS34_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRP86695.1"
                     /translation="MTRNFRAHRSILVLATLAGLLASAPVRATDAGTTETAEEFLARL
                     NVEFTELRLEVAKAFWVQSTYITPDTGFLAAKANEKLLAFTSRAIAESTRFIGQDLDP
                     DTARQLDLLRRGTTMPAPNDPAARAELAKLATEMEAMYGSAKVCPAEGECLDETAIIR
                     LMARERDYQRLLDAWRGWHDTAAPTRAMYERFVELANEGAVEIGFADLGELWKSGYDM
                     PPADFEAEAERLWQQVKPLYEQLHCHVRDKLADHYGEDRVPREGLIPAHLLGNIWAQE
                     WGNLYELLEPVPGVAQFDLDGTLESQGWDEIRMTRVAENFFVSLGMPPLPDSFWERSL
                     FRKPQDRDVVCHASAWPMDGGDDVRLKQCIEPTAQHLTTLHHELGHIYYFLLYKDQPP
                     LYQDGAHDGFHEGIGDTLELSMTPAYLREIGLIDEIEQNPDATLNLQMRLALDKIAFL
                     PFGKLIDQWRWDVFAGRTPPGQYNAAWWQLRADYQGIAPAVARDESDFDAGAKYHIPG
                     NTPYTRYFLARILQFQFHRALCEAAGHVGPLHECSIYGSEEAGRRLIAMLEMGASRPW
                     PDALELLTGSREMDASAIIDYFQPLMSWLETQNAGRSCGW"
     gene            7759..8670
                     /locus_tag="PLS34_04150"
     CDS             7759..8670
                     /locus_tag="PLS34_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRP86696.1"
                     /translation="MDAAPGRLAIVAAFAIVYVVWGSTYLAIRIAVQDLPPGLLAGIR
                     FIIAGVVLGALALARGQSLPRSMAEWRTTAILAVAFIVLGNGVVTWAEQWVPSNQAAL
                     IIASSALFTAWFGTFGRRAVPLSRATRVGLFAGLAGTALLVWPEASADAAPGGDLLWP
                     KAAILLSTLAWSWGVMFSRNSTVTMKPLMFAACQMLIGGSLLTLIGLANGELARWTWT
                     LPGIGGLLYLTVFGSCIAYATYMWLLNETTPARLGTVAYVNPAIATLLGWWVLDESLS
                     GLQLGGMAIILASVAWVTITARAIGRR"
     gene            complement(8723..9097)
                     /locus_tag="PLS34_04155"
     CDS             complement(8723..9097)
                     /locus_tag="PLS34_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRP86697.1"
                     /translation="MTGFLVRMLITAVSLWLAALLVPGMDLHGVGTVLGAALLLGIVN
                     AFVRPVVVLLTLPLTILTLGLFLLVVNAMMLGLVSVMFAGFTLGGFWSAMLGAVFVSF
                     FSWLGSAFIGRNGRYEVLVVRR"
     gene            complement(9108..9830)
                     /locus_tag="PLS34_04160"
     CDS             complement(9108..9830)
                     /locus_tag="PLS34_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HRP86698.1"
                     /translation="MASPSEEPAIVSDQPQRRESVLLSLGINIAIPAVILMKFSGENA
                     LGPVWGLVVALTFPLGYGIVDFARRREWNIVSILGFVSVLLTGGIGLLQLDPKWIAVK
                     EAAVPAVIGIAVLLSLRTRYPIVRTFLYNDKVIRVGEVDEALQRHGNREAFERTLVNA
                     SWMLAASFFVSAVLNFVLAKIIVKSPPGTTAFNEELGRMTALSYPVIVVPSMIIMVAA
                     FWYLFSRIRRLTDMDLEQILKT"
     gene            complement(9832..10728)
                     /locus_tag="PLS34_04165"
     CDS             complement(9832..10728)
                     /locus_tag="PLS34_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRP86699.1"
                     /translation="MQPDARRARLLRLATTASVATAAMLVAAKLGAWLLTGSVSLLAS
                     LVDSMMDVLASAVNLAAVRWSLLPPDEEHRFGHGKAEALAGLGQAAFIAGSALFLLFH
                     VIDRLLNPQPLEHLAVGLGIMAFSMVATALLIGFQRYVIRHTGSLAIRADSLHYLSDL
                     LTNAGILLALGLVLAGWAWADPLIGLAVAAYILWSAIRIGLDGIDVLMDRELPAEDQE
                     RIVAIASATPGVLGVHDVRTRQSGATRFIQLHLELPADLRLAESHALVSRVQAALLQA
                     FPDADVMIHQDPVAPDRRPVSG"
     gene            complement(10733..11035)
                     /locus_tag="PLS34_04170"
     CDS             complement(10733..11035)
                     /locus_tag="PLS34_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRP86700.1"
                     /translation="MSNEGYHEPVDELSDETRDMHRAIISLMEELEAVDWYNQRVDAC
                     KDAELRVILEHNRDEEKEHAAMVLEWIRRRDPLFSKELKEQLFTTGPIGHHGEHEH"
     gene            complement(11080..11895)
                     /gene="ppk2"
                     /locus_tag="PLS34_04175"
     CDS             complement(11080..11895)
                     /gene="ppk2"
                     /locus_tag="PLS34_04175"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRP86701.1"
                     /translation="MPAGRPKSWYSNTGALKRKAYEEELAQLQIELVKLLAWVRHRKL
                     KVVVIFEGRDAAGKGGAIKRVTEKLNPRACRIVALPTPTERERGQWYFQRYVAHLPTA
                     GEMVLFDRSWYNRALVERVMGFCDNDEYLEFARAVPEFERMLVRSGIILIKYWFSVSD
                     EEQERRFQARIHNPEKRWKLSPMDLESRRLYREYSQAKDDMLQFSDIPEAPWWMIPAD
                     DKRRARLNCIAHLLGSFAYEDVMPDPPSLPPRSNGHHHERPPIAEQNFVPIVY"
BASE COUNT         1924 a         4044 c         4057 g         1939 t
ORIGIN      
        1 tggattcatt gatggcaatc cggtaaatcc agctgtagaa cctgtgcccg aagtcgaatt
       61 gctcgagttt ctcgaacgcc ttgagaaacg tggtctgcgc gacatccctg gcgtcttccg
      121 gatggcgcag catccggatc gcgacgttgt aaaccggccg ttcgtagcgc gccagcagag
      181 tctcgaaggc ggcacggtcg ccgttcctgc acgctcttac cagtctttcg tccgggtcct
      241 tactcataca tacggcgcac tggacaaaaa gttggcgggc gtcgcgaggg ctaatcggcg
      301 cctggcgacg gctcgatcac gaagatcttg tcgaacccgg aatagcggaa gacgtcgcgg
      361 atgtgattgc cgacgttcac gagccgcaac gcgccgccct ggctcatgac ccgcttctgc
      421 gtgcgcagca ggatgccgag cccggcgctg gaaatgtact cgagcctcgc gaggtcgaac
      481 tcgcgcgggt tctcgatgcc gtcgatgaac tcttgcgccc tgcgggcctg cgaggcgtcg
      541 aggcgcccgg agatcacgat cctgccctcg cccctggatt ggatatcgaa catgctgcta
      601 gcccaggttc ttggtgaaag tcacggtggt tactccgtcc cggtggacgt agtccagcgt
      661 gtcgaccatt tgctgcacga ggtacaggcc cagcccgcct gtctcgcgct gctccacggg
      721 tgcgttcctg tcgaccaggc ggggcacggt gacgtcgaag ggctcgaccc ggcgatcgca
      781 caagcgtacc cggaccgccc ggtcggcatg ctgcagttgc agcgagatgt gctcgtcacc
      841 atccggattg aacttcacca tattggtgaa cagctcctcg accgcgagat tgaccgtgta
      901 cagctgctcc ggatcgatcc ccgctgcggc gaagaactcc tccgtcaggg cgaaaatctc
      961 cgccagcgag tcgaaatcgc gcctgaacag tcgctccatc tccgctcccc ctgccgtctc
     1021 gcgagtatac gccgcgaaaa ccgcgttcga gttccacccg ccgcaggcta ctccatgccg
     1081 ggcagcctgc gcagcaatac gatggtgacg tcgtcggcct gcgccgcgcc gccgccgaac
     1141 tcacgcaatg ccgcgtgcag ctcacgcgcc gcctcgcgca tcggccgatg ctgcagctgc
     1201 cgcagcaggt cggcgacccg ttgctcgccg aacggccggc gcgccgcgtc ctcgtattcg
     1261 tagacgccgt cggaaagtag cgcgaacagg tcccccggcg cgaactcgat ggtctttgcg
     1321 gggaacggct cggccagcgg catggcgccc atcgggatcg tggtggcgtc gtgccaggcg
     1381 gcctcgccgc cggcggcatg caggtgcagc aacggcgcct ggcccgcggc atgaaaccgc
     1441 agggtgtggc tgcgggcgtc cagcatgccg aggaaggcgg tgacgaagcg gtcgtccggc
     1501 aggtcgtcca gcagctggtt gttcacgtgc gtgtggatat cgtcgaggtc cgcacccagc
     1561 cgcagagcca cgcggatcat ggcgcggacc tgggtggccg agagcgccgg accgatgccg
     1621 tggccggtgg cgtcgccgag cagcaggaac atgccgtgtg tgcccacggg aacgaagtcg
     1681 aacaggtcgc cgcccgtctg gtccgtcggc agaaagaggc cggcgccgtc gtacccggcc
     1741 agcggcggca ttacgtccgg caggccgctc atctggatct cgcgggctac ccggatttcc
     1801 tcgtgcaggt ggtcggccgc gagccgctcc tcgaccgcct gcgcccgttg cagcgcgctg
     1861 gcggactgcg cggcgagaat cgatccgacc agctcgtcgt gggcatcgaa ggtgccgccg
     1921 cgcttgttga gcacctgcag cacgccgaac agccggccgt cctggtggag caggggcagg
     1981 ctcagcaggc agcgcgtgtg gaagccgctg agccgatcca ccgcggcgtt gaagcgcggg
     2041 tcggcggcgc agtcgggcac gttgaccagg cgccgctcgc gcgcgcagtg ccccacgatg
     2101 ccccggccgt tgggcacgcg gatcggcggc atgtcaccga cgaccgtcat caccagctcg
     2161 tcggtggcgg ggtcgagcag gaacaccgag ctgcgctccg catcgagcag gcgggttgcg
     2221 gccgccgcta cctcgtcgag gatcgcgagg tgctcgatcg gccggcccag ttccaccgtg
     2281 gcctcgagga tgcccgccag cgtctcggac ccgagctgtc gcgccgcgcc cgtactcatg
     2341 cggccgccgt ggcagcaagt ggtcggcccg gcgactgccg tcgcatatgc gccaatcggc
     2401 ccacgagtcc tcccccaaag acccccgggg aaggaaaata gcacgctttc ccgcatgttg
     2461 cggcgcaatt cacgccgccg cgagcgggcc tcagggcgtc gctttctgct ccgccgccgc
     2521 gaacttctcc agctgttcgg gcgtggcctc gcgctggtgg cgccgcttgt actcgccgta
     2581 gggcatgccg tagacgatct cgcgcgcctg ctcgtagtcc agcaccacgc ccgcctcggc
     2641 cgccgcgccg acgtaccact tcgacaggca gttccggcag aagtaggcca ggttcatcat
     2701 gtcgaggttc tggacctctg gatgggcgcg gaggtgctcc accagcttgc ggaacacgcg
     2761 cgcttcgatc tccgtgcggg cggcctcgtc cgccacgatg cggtcattca ttctgcatct
     2821 ctccctgcgg gccggctcgg cggcatcagc gccggaacac gctgtccgcc ggcacgacct
     2881 cgatcgagtc cgcgccgaac ccgagcggca tgacctcgat gacggcgtcc tcggcgagca
     2941 gcgcctccgg cttcggcgag ttgtagctca tcggcgagcg ctcgttgccg agcaggcggc
     3001 gcttcaggtc ggcagcgtcc ggcgtcacga acacgaactt gatgtccctc gtcgacagcc
     3061 gctcgcggat gacccggttg acgtcgtcca gcgtgagccc ggccagcgcc tggcgcacgt
     3121 aggcgggaaa gtcgggaatg ccgtaccagt cgctgtcgat ggcatagccg agcagagccg
     3181 gctgcgagct gaccagctgg gccacccact tgtcgaggaa tgcccgcgtg gcctcgaact
     3241 ccgcctcgct gaggccctgc tcgatcagcc ggtccagctc gtacagggcg gcccgggtgg
     3301 cgaagtgcgc ctcgttgttg ccggtcagcg gccgcagcca gacctggaag atctgctgcc
     3361 gccggaacag gtttgcatcc ggctgcgtca ggtacatgcc gcgcgggaag tactcgatgt
     3421 aggcgtagtt gccgtagttc atgccgcgct gttcgcgaat ggcctggaac agccgcgcgt
     3481 tctggctgcg atgctggccc agccactggg tcgcgagcca cagcggcacc cagtccggat
     3541 cgccgcgctt gagctcgatc gggaagccga aggagaccgc gtgcgccatg gtgtccttct
     3601 ccaggatcag cgcctggtga ccgcggaccg gagccgggct gcccggcgat gccggtgcgg
     3661 ccgccggatc aaccgtgggc aggcgcgcca ggtcgcggcg cagcgtggcg acgaactccg
     3721 cgggatagcc gccggccagg cccacggtga gattcgccgg gtggtagtgc gccgcgtaga
     3781 acgcctgcac gtcgtcgagc gtgagcgcct ggacgtcctc cacccggccg agcgtcagcg
     3841 tgccgtaggg atgttccggt ccgtagatga actggtagag cgcttccttg ccgagctctt
     3901 cgtcgttgtt ggccaccagg tcggtgagaa tggcgttgac cagctgggtc tgcagccgcc
     3961 ggaagtcctc ctcgcgccag ccgggggcga gcagctgctc ggccgtcagg ccgtaccagg
     4021 ccgccaggtt gtcacgatgg acggtgccgg acagccgcac catttccttg tcgacctgcg
     4081 cgccgaagcc ggccgccatg ggatagaggg cgtcgttgat ctggtcgatg gagcgcgacg
     4141 ccgagccggc ctccgtcagc atcatcgcgg tcagctgcgc gaggcccttc ttgcccggcg
     4201 ggtccagcat ggcgccggcc cggaagacat acgagacgtc caccagcggc gaggcgtcgc
     4261 tgtgccgttc caccaggcgg aaggcggcct cgccggtggt cgccgcggcg gccagggcat
     4321 cgaccgaggg gccgccggct tccagcccgg cgagggaatc gccgctggcg atgatgccga
     4381 cggtgcgcac cgcgtcacgg aagtgccggt cggccgccgc cgtcaggtcg gctggcgcca
     4441 gcgcggcaaa ggtcgcgtac agggcgtcga ccgtctcgac gctgcgctcg tactgcacga
     4501 agcccgcaag ggcggcgccg atcgccggcg aattgtcgag gccggcgagg aatccgaagc
     4561 gcatgcggtc cttgatgcgc tgcagcttgt ccggcgagag cgtctccgtg cgggcgcgcg
     4621 cgaaggtcgc gaggatctcg tcgcgcactt cggcggtcgc ggccgggtcg acgactcgcg
     4681 ccagcaccca gaacagcccc ggatcgcggt tcatcggcat gtaggcgaac agctgatcga
     4741 ccacctggcg ctccagcacc agctttcgat aaaggtcgga gttgtccgaa aaatagtgct
     4801 gcgcgatcat ctgcaccgcc gccgtgtccg ggtcgcgcgg atcgaagccc ggcccgcgga
     4861 acgccaccgc gatccacggc tgggtcggca tgtccaccgg cacgtacacc gaggtgggcc
     4921 cctcgccggg cggctcgacc gggatctccg cctggtagct gccgcgctgc cacggcccga
     4981 agtgcttttc gaccagcgca aaggtcggct cgaactcgag gtcgcccacc aggatcaccg
     5041 ccgacttctc gggccggtac cagcgatcga agaacagcct ggagtactcg tactgctccg
     5101 gcatctccag gatgtcctcg aagaagccca tggtggtgtg gcggtaggtg tgctcgcggt
     5161 aatgggtggc gcgcacctgc tcgaaaatct tgcggatcgg gttggcactg ttcttgatgt
     5221 actcgccctt gaccgccagc gcctcggtgc ggaacacgtc ctcgccgtag gacaggttca
     5281 tgaagcggtc cgcctccagc gccagcactg tgtcgaggtc ggccttggtg aagtcgatgt
     5341 gatagttggt gtaatcgtca gtggtgtagg cgttggtgtc ggcgccggcc agcttcaacg
     5401 cggcgttgta ctcctccggc gacacgttct cggtgccgcg gaacatcatg tgctcgaaga
     5461 agtgggcgaa gccggtcttg ccgggctcga cctcgttccg cgagccggtc tgcacgggaa
     5521 tctgcaggct gacgatgtcg gggaagtcgg tcttgacgac gatcaccttc aagccgttgg
     5581 cgagctcgcg ctcctgccag ggcaggtcga agaccctggc cttgtcgtcg ccaccggcgg
     5641 cctcgacagc ggcgcccgca ttgccgcctg caccggcggg cggggtcgcg caggcgacgg
     5701 tcaacagcat cgccagcatg gcgacggaaa gtttcacggg caggtggctt gtcatgactt
     5761 ctccaggcgt cggtatgccg gcggcggacg gtggcgcatt atagcgccgg ccccgggccg
     5821 gatggcgcgg gctgcgccgg gcagtacaat gccggcacgc cccgcggggc cccgtttcaa
     5881 cctgccagga tgtcagccga tgaccaggaa ctttcgcgcg caccgcagca ttctcgttct
     5941 cgccacgctc gccggcctgc tcgcctcggc gccggtccgc gccaccgacg caggcaccac
     6001 ggaaactgcc gaggaattcc tggcccgcct caacgtcgag ttcaccgagt tgcgcctcga
     6061 ggtagccaag gccttctggg tgcagtcgac ctacattacg ccggacaccg gcttcctggc
     6121 cgccaaggcc aacgaaaaac tgctggcatt caccagccgc gcgatcgccg agtcgacgcg
     6181 cttcatcggc caggacctcg acccggacac ggcgcgccag ctcgacctgc tgcgccgggg
     6241 caccaccatg ccggcgccca acgacccggc cgcgcgcgcc gagctggcga agctggccac
     6301 cgagatggaa gccatgtacg gctcggccaa ggtctgtccc gcggaggggg agtgtctcga
     6361 cgagacggcc atcatccgcc tgatggcgcg ggagcgggat taccagcggc tgctggacgc
     6421 ctggcgcggc tggcacgaca cggctgcacc gacacgggcg atgtacgagc gcttcgtcga
     6481 actcgccaac gagggtgcgg tcgagatcgg cttcgcggat ctcggcgagc tctggaaatc
     6541 aggttacgac atgccgccgg cggatttcga ggccgaggcg gagcgtctgt ggcagcaggt
     6601 gaagccgctg tacgagcagc tgcactgtca cgtccgggac aagctggccg accactacgg
     6661 cgaggaccgg gtgccgcgcg aggggctgat tccggcacac ctgctgggca acatctgggc
     6721 gcaggaatgg ggcaacctct acgagctgct ggagccggtc cccggggtcg cgcagttcga
     6781 cctcgacggc acgctggagt cacagggctg ggacgaaatc cggatgacgc gcgtcgccga
     6841 gaacttcttc gtgtcgctcg gcatgccgcc gctgcccgac tcgttctggg agcgttcgct
     6901 gttccggaag ccgcaggatc gcgacgtcgt ctgccatgcc agcgcctggc cgatggacgg
     6961 cggcgacgac gtgcggctga aacagtgcat cgagcccacg gcgcagcacc tcaccacgct
     7021 ccaccatgag ctggggcaca tctactattt cctgctctac aaggaccagc cgccgctgta
     7081 ccaggacggg gcccacgacg gcttccacga gggcatcggc gacacgctgg agctgtcgat
     7141 gacgcccgcc tacctgcgcg agatcggcct gatcgacgag atcgagcaga atccagacgc
     7201 gacgctgaat ctgcagatgc ggctggcgct cgacaagatc gccttcctgc cgttcggcaa
     7261 gctgatcgac cagtggcgct gggacgtgtt tgccggccgc acgccgccgg ggcagtacaa
     7321 cgcggcgtgg tggcagctgc gcgccgacta ccagggcatt gcgcccgccg tggcgcgcga
     7381 cgagagcgat ttcgacgccg gggccaagta ccacattccg ggcaacacgc cgtacacgcg
     7441 ctatttcctg gcgcgcatcc tgcagttcca gttccatcgc gccctgtgcg aggcggccgg
     7501 gcacgtcggc cccctgcacg agtgttccat ctacggcagc gaggaagccg gacgccgcct
     7561 gatcgccatg ctggaaatgg gtgccagccg gccctggccg gacgccctgg agctgttgac
     7621 gggatcgcgc gaaatggacg cctcggccat catcgactat ttccagccgt tgatgagctg
     7681 gctggagacc cagaacgccg ggcgcagctg cggatggtga ggcgctcgcg ccggggcgac
     7741 ggtccgccgt cctgctgaat ggacgccgca cccggccggc tggccatcgt cgcggccttc
     7801 gcgatcgtct acgtcgtctg gggctcgacc tacctggcga tccggatcgc ggtgcaggac
     7861 ctgccgcccg ggttgctggc cggtatccgc ttcatcattg ccggcgtcgt gctcggcgcc
     7921 ctggcgctgg cgcgcggcca gtcactgccg cgctccatgg ccgagtggcg caccaccgca
     7981 atcctcgcgg tggccttcat cgtgctcggc aatggcgtcg tcacctgggc cgagcagtgg
     8041 gtgccgtcca accaggcggc gctgatcatc gccagttcgg cgctgttcac cgcctggttc
     8101 ggcacgttcg ggcgccgtgc ggtgccgctc agccgcgcga cccgggtggg gctgtttgcc
     8161 ggcctcgccg gcactgcgct gctggtctgg ccggaggctt cggcggacgc ggcgccgggc
     8221 ggggacctgc tgtggccgaa ggcggcgatc ctgctgtcga cgctggcgtg gtcctggggc
     8281 gtcatgttca gccgcaactc gaccgtgacc atgaagccgc tgatgttcgc cgcctgccag
     8341 atgctcatcg gcggcagcct cttgacgctc atcggcctgg cgaacggcga gctggcacgc
     8401 tggacctgga ccctgccggg catcggcggg cttctgtacc tgacggtgtt cggctcctgc
     8461 atcgcctacg ccacctacat gtggctgctc aacgagacca cgcccgcccg gctgggcacg
     8521 gtcgcctacg tcaacccggc cattgcgacg ctgctgggct ggtgggtgct cgacgaatcg
     8581 ctcagcggcc tgcaactcgg cggcatggcc atcatcctcg ccagcgtcgc ctgggtgacc
     8641 atcaccgctc gcgccatcgg tcgccgctga gggaattggg gacattcccc aattggccaa
     8701 ttggggaatg tccccaattc cctcagcgac ggacgacgag gacctcgtaa cggccgttcc
     8761 ggccgatgaa ggccgagccc agccagctga agaagctgac gaagaccgcg ccgagcatcg
     8821 cgctccagaa gccgccgagc gtaaagccgg cgaacatcac ggacacgagc ccgagcatca
     8881 tggcgttcac caccagcagg aacaggccga gcgtcaggat cgtcagcggc agcgtcagca
     8941 gcacgaccac cggccgcacg aaggcattta cgatgccgag cagcagcgcc gccccgagca
     9001 cggtgcccac accgtgcagg tccatgccgg gcacgagcag cgccgccagc cacagcgaca
     9061 cggcggttat cagcatacgc accagaaacc cggtcatcgg cacctcctca agtcttgaga
     9121 atctgctcca ggtccatgtc cgtcaagcgc cggatccggc tgaacaggta ccagaaagct
     9181 gcgaccatga tgatcatgga cggtaccacg atgacggggt aggacagcgc cgtcatgcgg
     9241 cccagctcct cgttgaaagc ggtcgttccg ggggggctct tgacgatgat cttcgccagc
     9301 acgaagttca gcacggcaga gacgaaaaac gaggccgcca gcatccacga ggcattcacc
     9361 agcgtgcgct cgaaggcttc ccggttgccg tggcgctgca gcgcctcatc gacctcgccg
     9421 acgcggatga ccttgtcgtt gtacaggaag gtcctgacga tgggatagcg cgtgcgcagc
     9481 gacaggagca cggcgatgcc gatcaccgcg ggcaccgccg cctccttgac tgcgatccat
     9541 ttcgggtcca gctgcagcag gccgataccg ccggtgagca gcacgctcac gaagccgagg
     9601 atggagacga tgttccattc ccgccggcgg gcgaagtcga cgatgccgta gcccagcggg
     9661 aacgtgagcg cgacgaccag gccccagacg ggacccagcg cgttctcgcc cgagaacttc
     9721 atcaggatga cggcggggat cgcaatattg atccccaggg aaagcagcac gctttcgcgg
     9781 cgctgcggct ggtcgctcac tatggcgggt tcctcggagg gggacgccat tctagccgga
     9841 aaccggccgc cggtcgggcg ccacggggtc ctggtgaatc atcacgtcgg cgtccggaaa
     9901 agcctgcaac agcgcggcct ggacccgcga caccagggcg tgcgactccg ccaggcgcag
     9961 gtcggcgggc agctcgagat gcaactggat gaaccgggtc gcgccggatt ggcgggtgcg
    10021 cacatcatgc acgcccagca cgccgggcgt cgcggaggcg atcgcgacaa tccgctcctg
    10081 gtcctccgcc ggcaactccc ggtccatcag cacgtcgatg ccgtccaggc cgatccggat
    10141 ggcgctccag aggatgtatg cggccaccgc caggccgatc agcggatcgg cccaggccca
    10201 gcccgccagc accaggccca gcgccagcag gatgccggcg ttggtcagca ggtcggacag
    10261 gtagtgcagg gaatccgccc ggattgccag cgagccggtg tgacggatga cgtagcgctg
    10321 aaagccgatc aacagcgccg tcgccaccat cgagaaggcc atgatcccga gccccacggc
    10381 gagatgctcc agcggctggg ggttcaacag ccggtcgatc acgtggaaca gcagaaacag
    10441 cgccgagccg gcgatgaagg cggcctgccc gaggccggca agcgcctccg ccttgccgtg
    10501 gccgaagcgg tgttcctcat ccggcgggag cagcgaccag cgcacggcgg cgaggttcac
    10561 cgccgaggcc agtacgtcca tcatcgagtc caccagcgag gcaagcaggc tgacggagcc
    10621 cgtgagcagc cacgcgccca gcttggccgc gaccagcatg gcggccgtgg cgaccgatgc
    10681 ggtcgtggcg agcctcagca gccgggcgcg gcgcgcgtcc ggttgcaggc gctcagtgtt
    10741 cgtgttcgcc gtgatggccg atgggaccgg tggtgaacag ctgctccttg agctccttgg
    10801 agaagagcgg atcgcgccgc cggatccatt ccaggaccat cgccgcgtgc tccttttcct
    10861 cgtcgcggtt gtgctcgagg atcacccgga gctccgcatc cttgcatgca tccacgcgct
    10921 ggttgtacca gtccactgcc tccagctcct ccatcagtga aatgatggcg cggtgcatgt
    10981 cccgggtctc gtcggagagc tcgtcaaccg gttcgtggta tccctcgtta gacatatcgt
    11041 gtccctcggt ctctggtcag tgtgcaatcc acgccgccgt caatagacga tcggcacgaa
    11101 attctgctcg gcgatcggcg gtcgctcgtg gtggtggccg ttgctccgcg gcggcagcga
    11161 gggcgggtcc ggcatgacgt cctcgtaggc gaacgacccg agcaggtgcg cgatgcagtt
    11221 gagacgcgca cgccgtttgt cgtccgcggg aatcatccac cacggcgcct cgggaatgtc
    11281 tgagaactgc agcatgtcgt ccttggcctg cgagtactcc cggtacaggc gccgcgattc
    11341 gaggtccatg ggcgacagct tccagcgttt ctccgggttg tgaatccggg cctggaaacg
    11401 gcgctcctgc tcctcgtcgc tcaccgagaa ccagtacttg atcaggatga ttcccgagcg
    11461 caccagcatc cgctcgaatt cgggcacggc gcgggcgaac tcgagatact cgtcattatc
    11521 gcagaagccc atcacgcgct cgaccagcgc gcggttgtac cagctgcggt cgaacagcac
    11581 catttcgccg gcggtgggga ggtgggcgac gtagcgctgg aagtaccact ggccgcgctc
    11641 ccgctcggtg ggtgtaggca gcgccacgat gcggcaggct ctcgggttca gcttctcggt
    11701 cacgcgcttg atggcgccgc ctttgccggc ggcatcgcgg ccctcgaaga tcaccacgac
    11761 cttgagtttc cggtgccgca cccaggccag caacttgacc agctcgatct ggagctgggc
    11821 cagttcttcc tcgtaggcct tgcgcttgag cgcgccggtg ttgctgtacc agctcttcgg
    11881 gcggccggcc ggcagggcgt acaggtcgct caggctgtgc accgggcaga tcgccgtgcc
    11941 gctgctctcg agggtcgcct gctt
//
LOCUS       DAOMPG010000049        11887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2330_length_11887_cov_3.934753, whole genome shotgun sequence.
ACCESSION   DAOMPG010000049 DAOMPG010000000
VERSION     DAOMPG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2330_length_11887_cov_3.934753"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..>1209)
                     /gene="purF"
                     /locus_tag="PLS34_04180"
     CDS             complement(8..>1209)
                     /gene="purF"
                     /locus_tag="PLS34_04180"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006010991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRP86702.1"
                     /translation="NLTNADELADLVIREDHRYLNTSSDSEVLLNVFAHELEQVDGRF
                     PGPDEVFRAAEGIYRRCRGGYAVVALIMHTGILALRDPCGIRPLVLGYRDVGGRREHM
                     VASESVALDVAGFELLRDVLPGEAVFIDLQGRLHTRQCVPAERRSPCIFEYVYFARPD
                     SIIDNISVYKARLRMGEALADRIIAQRPEHDIDVVIPVPDTSRTSALPLAHRLGVKYR
                     EGFIKNRYIGRTFIMPGQTLREKSVRRKLNAIDLEFRKKNVLIVDDSIVRGTTSRQII
                     DMAREAGARKVYFASAAPPVRYPNVYGIDMPAAAELIAHGRTEQEVQEYIGADWLIYQ
                     TLEDLVRAVKHDNADIREFDTSCFSGEYLTGDVTSEYLHKIETARSDSAKTARRPVVA
                     GRTAEIL"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1220..1481)
                     /locus_tag="PLS34_04185"
                     /pseudo
     CDS             complement(1220..1481)
                     /locus_tag="PLS34_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251798.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
     gene            complement(1489..1983)
                     /locus_tag="PLS34_04190"
     CDS             complement(1489..1983)
                     /locus_tag="PLS34_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRP86703.1"
                     /translation="MVIVDYVLLGAFAVSVIIGFFRGFFREALSLVNWALALWLAWRF
                     SGLLDPLLASVSSPVLKLWLGRVVVFVGALLAGGLIGHLIVLLVRKSGLTGVDRTLGM
                     VFGAGRGVLVVGALVIVFQMLEMDQESWWQESVIVPRTAELTKALRDYIDAGLDWAGD
                     AMAE"
     gene            complement(2005..2649)
                     /locus_tag="PLS34_04195"
     CDS             complement(2005..2649)
                     /locus_tag="PLS34_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP86704.1"
                     /translation="MERHLKERIVGATVIVALGIIIIPWLLDGPAQSSRPVEQPIELP
                     PVEQPGRTYTIPLDPAAGPAVPVDSGNGQPTQTRPAAGTIQRPSDDPPAAPVRQPDPQ
                     VTRPAEQPAQPPPRPSPTPQPAPAAQPPAPAADAEAWTVQVGSFSQADNAQALQARLK
                     AEGFESFISRVATDAGTMHRVRVGPVPERAEADRLLTRLRAAGHGGARVVRAQD"
     gene            complement(2678..3949)
                     /gene="folC"
                     /locus_tag="PLS34_04200"
     CDS             complement(2678..3949)
                     /gene="folC"
                     /locus_tag="PLS34_04200"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HRP86705.1"
                     /translation="MADKDLAAWLAWQERLHPREIDLGLERVRAVLGAMGLRAPPYRI
                     VTVGGTNGKGSCVATLEALASEAGLLTGAYTSPHLLRYNERVRIGGAEAGDAELVAAF
                     EAIDAARGDTSLTYFEFGTLAALEIFRRRGVELAVLEVGLGGRLDAVNAVDPEVAVVT
                     SVGLDHMEWLGPDRESIGREKGGIFRPGRPAVIGDRRPPASLLLAAGADALRLGLDFD
                     WREDGAGWCWQSAARRLAGLPRGPLAAPALLDNAACAIAAFAILEPGSATPGLAARAL
                     PRIALVGRLQRIPGQTEWWLDVAHNPDGAAVLAGALAAAPVRGRTLAVIGMLADKDVA
                     AVGAALDGGIDAWFAATLPPPRGLDATTLAARLGGHVQTPVRTAPSVAAACEAARAAA
                     RPGDRVVVCGSFHAVGPALEWLGVYCEPLTE"
     gene            complement(3957..4835)
                     /gene="accD"
                     /locus_tag="PLS34_04205"
     CDS             complement(3957..4835)
                     /gene="accD"
                     /locus_tag="PLS34_04205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRP86706.1"
                     /translation="MSWFDKLMPSRIKTQGGSQGRSRSVPEGLWTKCPACDAVLYRAE
                     VERSLFVCPKCSHHMRIDARTRLKMFLDPGTGRELGQSLQPEDPLKFRDSKRYRDRLV
                     QAQKATGESDAMVAMTGSLLGLRLAACAFEFQFMGGSMGSVVGERFRRAADAACDERM
                     PLVCFSASGGARMQEGLYSLLQMSKTAAALRRLGDAGMPFVSVMTDPTTGGVSASLAM
                     LGDVNIAEPKALIGFAGPRVIQQTVGETLPEGFQRSEFLLKHGALDMIVDRREMRDTI
                     ARLLSMLTGRPLQQPG"
     gene            complement(4927..5730)
                     /gene="truA"
                     /locus_tag="PLS34_04210"
     CDS             complement(4927..5730)
                     /gene="truA"
                     /locus_tag="PLS34_04210"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRP86707.1"
                     /translation="MTRPAPGYARIAVGVEYDGAGLFGWQRQKDGPSVQEALERALSA
                     VADETVEVVGAGRTDAGVHATGQVAHFDTTAQREARAWVLGANTVLPAGVCLRWATAA
                     PPQFHARFSAVARSYSYLLLNRQTRSALWRERAWWTRRPLDAALMHEVGQVLVGEHDF
                     SAFRAAECQSHSPVRRIDSLAVTRRGDFLRLDVTANAFLHHMVRNIVGTLAAVGRGHR
                     PPEWVADVLAGRDRCEAGATAPAEGLYLVRVDYGPLLPTPPAERPGPLA"
     gene            complement(5727..6443)
                     /locus_tag="PLS34_04215"
     CDS             complement(5727..6443)
                     /locus_tag="PLS34_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86708.1"
                     /translation="MRPAVVGTTPDEGPSGLHPAFRILLLLVLAAMLFRYSLAAMALV
                     LAALLAGAAWQGADTLRAILKSVRRIRWLLLSIVVIYLWVAPEPAVDGQPWYLPGWSD
                     LDMALRRSGVLVVLVSAVELLRRRTPAPRMAAGLVMLLSPLAWIGVDTGVFARRLALT
                     LDAVPLTAERVAQAAGRRQIKRGFAGWGEAAAALVRDIEAGSGQAPREAALPALGRPG
                     RRDWLVLAVGVAACLLAGQL"
     gene            complement(6447..9428)
                     /locus_tag="PLS34_04220"
     CDS             complement(6447..9428)
                     /locus_tag="PLS34_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HRP86709.1"
                     /translation="MSRKLILLGVLSLILAPGAALSLGLGDIRLNSYLNQPMNAEIAL
                     SIASPAELESLQIRLAPAAAFSRYGLDRPAFLDDLRFQVRRTGAASAVVQVTSTRPVA
                     EPFVTFLVEAAWSGGQMLREYTVLLDPPTFMPAPEAAPASPAAVDTPRQAPVARPAPA
                     PAPSAAPTPLRSEYGAEFGPVQRNDTLWAIAQRVRPDTGLSTNQVMVALFRANPNAFD
                     GNINRLRAGAILRVPSRSEMAATSGGEATAEVRRQNEAWRGAAAPVAERRLELAPPAD
                     TPPSAPAADPVRDATSPSPALAEAVDELRGELAETRRLMEVKDAEIAALQARLAELEE
                     EGVPVAELPIAPVERPIVEAEPADELPAEEGVPADAEAEAEPVAEPEPAPTPAAAPTA
                     PEPTLMDRIMALLGNLWLWLVLAVVLLAAAIVVFLRKRRDEERSIEEDLAETGTWTAL
                     EESGAKSGAAAVKVQPRPARQDDLESILVEEAERPAPARAAEPAPIPAPEAEPQNEDD
                     YQYPFEDTIAGETGINLDQTDPMAEADFHMAYGLYDQAAEIIKKAIEREPDRYDLRRK
                     LIDICFVWGNAQEFLNQAKAVRQLGGDAQAADWAKVAIMGRQICPGEPLFAVGDAPSV
                     DVDFGGEELDLPELGKDTGSSGEWLDFDVGESDEPVQALGDTREQPAPGARKPIEQTA
                     ELDLEELGIDLDLGESGQHALQDLGDLPEDEADDEAHVGTLRMSEPPDFGAAGQDEAA
                     DTRISPARDEDEEGGTLMMDTRELASGSDGATQRGERLDLSEDEPTLSGVTGLDEEDF
                     NDRTAVQQGPAVDEGDEIPTLSGLGALPEDEDDEGTLITRYEASDSSPTAEVGAIEQD
                     LDLDLDDLTQVLESDVEAGREADEDATQLARGPAGDGDATMMAPGLGFRGKDDDATLM
                     APAGDAPEGYDYQEGDTREMPPMEISADDVNEIGTKLDLARAYIDMGDPEGARSILEE
                     VLEEGDETQRDEARELLDSLA"
     gene            complement(9533..10561)
                     /locus_tag="PLS34_04225"
     CDS             complement(9533..10561)
                     /locus_tag="PLS34_04225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRP86710.1"
                     /translation="MSRKKGFSVAVVGATGLVGEAMLACLGERKFPVAELKLLASERS
                     AGTRIDFGGKSHRVAELASCDFSGVDIALFSAGGSVSKEHAPRAAAAGAMVIDNTSCF
                     RYDDDVPLVVPEVNAGALEQAFARNIIANPNCSTIQMVVALKPIYDAVGITRINVATY
                     QSISGAGKRMVDELARQTAQLMNGRPLGKTLTPRQLAFNVLPQIDVFEDNGYTREEMK
                     MCWETKKILADPEIGVNATAVRVPVFFGHSEAVHIETREKISAREARRLLRQAPGVEV
                     LDRRVPGGYPTAVTEASGADAVWVGRIREDISHPRGLSLWIVADNIRKGAALNSVQIA
                     ETFVKTYL"
     gene            complement(10579..11808)
                     /locus_tag="PLS34_04230"
     CDS             complement(10579..11808)
                     /locus_tag="PLS34_04230"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP86711.1"
                     /translation="MPRAERPTLAEHGTAGGLYPRLSAFYLFYFGAIGVFVPYFSFYL
                     QHRGFDAVAIGQLMAIPMAMRVLAPGLWGWAADRSGRRDLTLRVGALLAAGGAALLLG
                     ATGFVATALALAAFALPWSGLLPQFEATTLSHLGRRPQRYGHIRLWGSVGFILAVLLG
                     GWLFRGEQIGAVPVALLLVVLGTAVAVWRVPPAPAPVAAHVPGAFAAALRRPWVLALL
                     AVCILQQASFGPYYVFFTIYLDELGYGTRMAGLLWAWGVAAEILMFIYTPRLIGRFGF
                     RGLLVFALGASVLRWLLTAWAAASLPWLLFAQTLHMAAFGLFHAVAVLLVHRAFAGRL
                     QGRGQALYSAVGFGVGGAIGSLASGYAWSLGGADAAWIAAAVVAAVAMAVAWWGLRAG
                     EAGLDRGWGGADARRDG"
BASE COUNT         1758 a         4346 c         4112 g         1661 t
ORIGIN      
        1 tcccgcgcta caggatctct gccgtgcggc cggcgaccac gggccggcgc gccgtcttgg
       61 cggaatccga gcgcgccgtc tcgatcttgt ggagatattc ggacgtgacg tcgccggtca
      121 ggtactcgcc cgagaaacac gaggtgtcga actcgcggat gtcggcgttg tcgtgcttga
      181 cggcgcgcac caggtcctcg agcgtctggt agatcagcca gtccgcgccg atgtactcct
      241 gcacctcctg ctcggtccgg ccgtgcgcaa tcaattccgc ggccgccggc atgtcgatgc
      301 cgtagacgtt gggatagcgc accggcggcg cggccgaggc gaagtacacc ttgcgggcgc
      361 ccgcctcgcg cgccatgtcg atgatctgcc tggaggtggt gccgcgcacg atggagtcgt
      421 ccacgatcag cacgttcttc ttgcggaact cgaggtcgat ggcgttcagc ttgcggcgca
      481 cggatttctc gcgcagcgtc tggcccggca tgatgaaggt gcgaccgatg taacggttct
      541 tgatgaagcc ctcgcggtac ttgacgccga gccggtgcgc cagcggcagc gcactggtgc
      601 ggctggtgtc cggcaccggg atgaccacgt cgatgtcgtg ctcgggccgc tgcgcaatga
      661 tgcggtcggc gagcgcctcg cccatgcgca gccgcgcctt gtagaccgag atgttgtcga
      721 tgatcgaatc gggccgcgcg aagtacacgt actcgaagat gcaggggctg cggcgctccg
      781 cgggcacgca ctggcgcgtg tgcaggcgcc cctggaggtc gatgaacacc gcctcgccgg
      841 gcagcacgtc gcgcagcagt tcgaagcccg cgacgtcgag cgccacgctt tccgaggcca
      901 ccatgtgttc gcgccggccg cccacgtcgc gataaccgag caccagcggc cggatgccgc
      961 agggatcgcg cagggcgaga atacccgtgt gcatgatcaa ggcgaccacg gcgtagccgc
     1021 cgcggcaccg gcggtagatg ccctctgcgg cgcgaaacac ctcgtcgggt cccgggaatc
     1081 tgccgtccac ctgctcgagc tcgtgcgcga acacgttcag cagcacctcg gagtcggagc
     1141 tggtgttgag gtagcgatgg tcctcgcgga tcaccaggtc ggcgagctcg tcggcgttgg
     1201 tcaggttgcn nnnnnnnnna gaagggctgc gcctcggtgc ggctcgaggt gcccgcggtc
     1261 gggtacctgc agtggccgat gccgatattg ccggtcagct tcagcatgtg gcggcgctcg
     1321 aacacatcgc gcaccaggcc gttgttcttg cgaatgtgca ggcgctcgtc ggcggtgacg
     1381 atgcccgcgg cgtcctggcc gcgatgctgc agcaccgtca gcgcgtcgta gagcacctgg
     1441 ttgaccgcgc cgttgccgac gatccccacg attccgcaca tcgctcgctt actccgccat
     1501 ggcgtcgccg gcccagtcca ggccggcatc gatgtaatcc cggagcgcct tcgtcagctc
     1561 cgccgttctc ggcacgatga ccgactcctg ccaccacgac tcctggtcca tctccagcat
     1621 ctggaacacg atgaccagcg ccccgaccac cagcacgccg cgcccggcgc cgaacaccat
     1681 gccaagtgtc cggtccacac ccgtcaagcc gctctttctc accagcagca cgatcaggtg
     1741 gccgatcagc ccgccggcca gcagcgcgcc gacgaacacc accacgcgtc ccagccagag
     1801 ctttagcacc ggcgaactga ccgacgccag cagcgggtca agcaggccgg agaagcgcca
     1861 ggcgagccac agcgccagcg cccagttcac cagcgacagc gcctcgcgga aaaagccgcg
     1921 gaaaaagccg atgatcacgg atacggcgaa tgcgccgagc aggacgtaat caacgatgac
     1981 catggcgcct cgcgtgctcg ttcatcagtc ctgcgcccgc actactcggg cgccgccatg
     2041 tcccgccgcc cgcaagcgcg tcagcagccg gtccgcctcg gcgcgttccg gcaccgggcc
     2101 gacacgcacc cggtgcatgg tgcccgcgtc ggtcgccacg cgcgatatga aggactcgaa
     2161 accctcggcc ttgagccgcg cctgcagtgc ctgcgcgttg tcggcctgcg aaaagctgcc
     2221 gacctgcacg gtccaggctt ccgcgtccgc agccggcgcc ggtggctgcg cggcgggtgc
     2281 cggttgcggg gtcggcgacg ggcgtggcgg cggttgcgcc ggctgctcgg ccggccgcgt
     2341 gacctgcgga tcgggctgcc ggaccggggc cgccggcgga tcatccgagg gccgctggat
     2401 cgtgcccgcc gcagggcgcg tctgcgtcgg ctgcccgttg ccggagtcga ccggcacggc
     2461 gggacccgct gccggatcga gaggaatggt gtaggtgcgc ccgggttgct ccacgggcgg
     2521 cagctcgatg ggctgctcga cggggcgcga ggactgcgcc gggccgtcga ggagccaggg
     2581 aatgatgatg atgccgagcg ccacgatgac cgtggcgccg acgatgcgct ccttcagatg
     2641 ccgctccatt ttgccagggc cgaaacgccg gccggcgcta ctctgtcagg ggctcgcagt
     2701 ataccccaag ccactccagc gccggaccca cggcgtgaaa agaaccgcac accacgacgc
     2761 ggtcgccggg gcgggcggcg gcccgcgccg cctcgcatgc ggccgcgacc gacggcgccg
     2821 tccgcaccgg ggtctgcacg tgcccgccga gacgcgcggc cagcgtcgtc gcatcgaggc
     2881 cgcgcggcgg cggcagcgtg gcggcgaacc atgcatcgat gccgccatcg agcgcggcgc
     2941 cgaccgccgc cacgtccttg tccgccagca tgccgatcac cgccagcgtg cgaccgcgga
     3001 ccggcgcggc ggccagggcg ccggccagca cggctgcccc atcgggattg tgcgcgacgt
     3061 ccagccacca ctcggtctgg cccggaatgc gctgcaggcg acccaccagc gcgatgcgcg
     3121 gcagcgctcg cgccgccagg ccgggtgtgg ccgaccccgg ctcgaggatc gcgaaggccg
     3181 cgatggcgca ggcggcgttg tcgagcagcg ccggcgccgc cagcggcccg cgcggcaggc
     3241 cggcaagccg ccgtgccgcg ctttgccagc accagcccgc gccgtcctcg cgccagtcga
     3301 agtcgagccc gagccgcaag gcatccgcgc ccgcggccag caacaggctg gcaggcgggc
     3361 ggcggtcgcc gatgacggcg ggccggcccg ggcggaagat cccgcccttc tcgcggccga
     3421 tgctctcgcg atcgggtccc agccactcca tgtgatcgag cccgaccgag gtcaccacgg
     3481 cgacttcggg gtccacggca ttgacggcat ccagccggcc gcccagcccg acctcgagga
     3541 cggcgagttc gacgccgcgc cggcggaaga tctccagcgc ggccagtgtg ccgaactcga
     3601 agtaggtcag gctggtgtct ccccgcgcgg cgtcgatcgc ctcgaaggcc gcgaccagtt
     3661 cagcgtcgcc ggcctcggcg ccgccgatgc gtacccgctc gttgtagcgc agcaggtggg
     3721 gcgaagtgta ggcgccggtg agcaggccgg cttcgctggc cagcgcttcg agcgtcgcga
     3781 cgcagctgcc cttgccgttg gtgccgccga cggtgacgat gcgatacggc ggggcgcgca
     3841 accccatcgc gccgagcacc gcgcgcacgc gttcgaggcc gaggtcgatc tcgcggggat
     3901 gcagccgctc ctgccaggcc agccaggccg caagatcctt gtcggccaag cgccgctcag
     3961 cccggctgct gcagcggccg gccggtgagc atggacagca ggcgcgcgat ggtgtcgcgc
     4021 atctcgcgcc ggtccacgat catgtcgagg gcgccgtgct tgaggaggaa ctcgctgcgc
     4081 tggaagcctt ccggcagggt ctcgcccacg gtctgctgga tgacgcgcgg gccggcgaag
     4141 ccgatcagcg ccttgggctc ggcgatgttg acgtcgccga gcatagccag gctggcggac
     4201 acgccgccgg tggtcgggtc ggtcatcacc gagacgaacg gcatgccggc gtcgcccagc
     4261 cgccgcagcg ccgcggcggt cttgctcatc tgcagcagcg agtacaggcc ctcctgcatg
     4321 cgtgccccgc cgctggccga gaaacatacc agcggcatgc gctcgtcgca ggccgcgtcc
     4381 gcggcccgac ggaagcgctc gccgaccacc gagcccatcg agccgcccat gaactggaac
     4441 tcgaaggcgc aggccgccag cctgaggccg agcaaggaac cggtcatggc caccatggcg
     4501 tcggactcgc cggtggcctt ctgcgcctgc acgagccggt cccggtagcg cttgctgtca
     4561 cgaaacttga gcgggtcctc gggctgcagc gactggccga gctcgcggcc ggtgccgggg
     4621 tcgaggaaca tcttgagccg cgtgcgcgca tcgatgcgca tgtggtggct gcatttcggg
     4681 cacacgaaca ggctgcgctc gacctcggcg cgatacagca cggcgtcgca ggcggggcac
     4741 ttggtccaca ggccctcggg gaccgagcgg ctgcggccct ggcttccgcc ctgggtcttg
     4801 atccgcgacg gcatcagttt gtcgaaccag ctcatgcgtt cccctcgccc gctgtgcttt
     4861 ggcgcaccct cgggcgcggc cgctcgccgc cgcccgcggg caggggccga tgataaacga
     4921 aacggctcat gcgagcggcc cgggccgctc ggccggcggg gtgggcagca gcgggccgta
     4981 gtcgacgcgc accaggtaca ggccctccgc cggcgcggtc gcgcccgcct cgcagcgatc
     5041 gcgtccggcc agcacgtcgg ccacccattc gggcgggcgg tgaccccggc cgacggccgc
     5101 cagcgtgccg acgatgttgc gcaccatgtg atgcaggaag gcgttcgcgg ttacgtccag
     5161 gcgcaagaag tcgccgcgcc gcgttaccgc gaggctgtcg atgcggcgca ccgggctgtg
     5221 cgactggcac tccgcggcgc ggaacgccga aaaatcgtgc tcgccgacca gcacctggcc
     5281 gacctcgtgc atcaaggccg catccagtgg ccggcgcgtc caccaggcgc gctcgcgcca
     5341 gagcgccgag cgggtctggc ggttgagcag caggtaactg tagctgcgcg ccacggcgga
     5401 gaagcgtgca tggaactgcg gcggcgcggc cgtcgcccag cgcaggcaca cacccgccgg
     5461 caacaccgtg ttcgcgccga gcacccaggc acgcgcctcg cgttgcgccg tggtgtcgaa
     5521 atgcgccacc tggccggtgg cgtgcacgcc cgcgtcggtg cgccccgccc cgaccacctc
     5581 cacggtttcg tccgcgaccg cggacaaggc gcgctcgagc gcttcctgca cgctcggccc
     5641 gtccttctgg cgctgccagc cgaacagccc tgccccgtcg tactcgacgc cgacggcgat
     5701 gcgcgcgtaa cccggcgcgg gcctggtcac agctggcccg ccagcaggca ggcggccacg
     5761 ccgaccgcca gcaccagcca gtcgcgccga ccgggacggc cgagggccgg cagtgcagcc
     5821 tcgcgcggcg cctgtccgct gccggcttcg atgtcgcgca cgagcgcggc cgcggcctcg
     5881 ccccagccgg caaagccgcg cttgatctgg cggcggccgg ccgcctgcgc cacccgctcg
     5941 gcggtcagcg gcacggcgtc gagggtcagc gcgaggcggc gcgcgaatac cccggtatcg
     6001 acgccgatcc acgccagcgg gctcagcagc atcaccaggc ccgccgccat gcgcggcgcg
     6061 ggtgtccgcc gtcgcaggag ctccacggcg ctgaccagca ccaccagcac gccgctgcgc
     6121 cgcagggcca tgtcgaggtc gctccagccc ggcaggtacc acggctgccc gtcgaccgcc
     6181 ggctccgggg cgacccacag gtagatcacg acaatcgaca gcagcagcca gcgaatgcgg
     6241 cgcaccgact tgaggatggc ccgcagggtg tcggcgccct gccaggcggc gccggccagc
     6301 aatgccgcca gcaccagcgc catggcggcc agcgaatagc ggaacagcat ggcggcgagg
     6361 accagcagca gcagaatgcg aaacgccgga tgaaggccgc tcgggccctc atccggcgtc
     6421 gtgccgacca ccgccggacg catccctcag gcgaggctgt cgagcagttc gcgggcctcg
     6481 tcccgctgcg tctcgtcacc ctcttcgagc acttcctcga ggatgctgcg cgcgccttcc
     6541 ggatcgccca tgtcgatgta ggcgcgggcg aggtccagct tggtgccgat ctcgttgacg
     6601 tcgtcggcgc tgatctccat cggcggcatt tcgcgcgtgt cgccttcctg gtaatcgtag
     6661 ccttccggcg cgtcgccggc cggcgccatc agggtcgcgt catcatcctt cccacggaag
     6721 cccagtcccg gcgccatcat ggtggcgtcg ccgtctccgg ccggtccgcg cgccagctgt
     6781 gtggcgtcct cgtcggcctc acggccggcc tcgacgtccg actccagcac ctgggtcagg
     6841 tcgtccaggt ccaggtcgag atcctgctcg atggcgccga cctcggcggt cggcgagctg
     6901 tcggaggcct cgtagcgcgt aatcagcgtg ccctcgtcat cttcatcttc gggcaaggcg
     6961 cccagcccgg acagcgtcgg gatttcgtcg ccctcgtcca ccgccggccc ttgttgcacg
     7021 gcggtgcggt cgttgaagtc ctcctcgtcg agaccggtga cgccggacag cgtgggttcg
     7081 tcctccgaca ggtcgagccg ctcgccacgc tgggtcgcgc cgtcgctgcc ggacgccagc
     7141 tcgcgagtgt ccatcatcag cgtgccgcct tcctcgtcct cgtcgcgggc cggggaaatg
     7201 cgggtatcgg cagcctcatc ctgcccggcg gcgccgaaat ccggcggctc ggacatgcgc
     7261 agggtaccga catgcgcctc gtcatcggcc tcgtcctccg gcagatcgcc gaggtcctgc
     7321 aaggcgtgct ggccggactc gccgaggtcg aggtcgatac cgagctcctc gagatcgagc
     7381 tccgcggtct gttcaatggg tttcctggcg ccaggcgccg gctgctcacg cgtgtcgccc
     7441 agcgcctgga ccggctcatc cgattcgccg acgtcgaaat ccagccactc gccggacgag
     7501 ccggtgtcct tgccgagctc cggcaggtcc agctcctcgc cgccgaaatc gacgtccacc
     7561 gaaggcgcgt caccgaccgc gaacagcggc tcgccggggc agatctgccg ccccatgatc
     7621 gccaccttgg cccagtccgc cgcctgggcg tcgccgccga gctggcggac cgccttggcc
     7681 tggttcagga attcctgggc gttgccccag acgaagcaga tgtcgatcag cttgcggcgc
     7741 aggtcgtagc ggtcgggctc gcgctcgatc gccttcttga tgatttccgc ggcctggtcg
     7801 tacaggccgt aggccatgtg gaaatcggcc tcggccatcg gatccgtctg gtcgaggttg
     7861 atgccggtct cgccggcgat ggtgtcctcg aagggatact ggtagtcgtc ctcgttctgc
     7921 ggctccgctt cgggcgccgg gatcggcgca ggctcggccg ccctggccgg cgccggccgc
     7981 tcggcttcct cgacgaggat cgactcgagg tcgtcctggc gtgcgggccg cggctgcacc
     8041 ttgaccgccg ccgcgccgga cttcgcaccg gactcctcga gcgccgtcca ggtgcccgtc
     8101 tcggcaaggt cctcctcgat ggaacgctct tcatcgcgcc gcttgcgcag gaacacgaca
     8161 attgccgcgg ccagcaacac cacggcaagc accagccaca gccagaggtt gcccagcagc
     8221 gccatgatgc gatccatcag cgtcggctcg ggcgcggtcg gcgcggctgc cggcgtcggg
     8281 gcgggctccg gctcagccac cggctccgcc tcggcctctg catctgccgg cacgccctcc
     8341 tcggcgggca gctcgtccgc cggctcggct tctacgatcg gccgctcgac gggtgcgatc
     8401 ggcagctccg cgaccggcac accctcctcc tcgagctcgg ccaggcgcgc ctgcaacgcg
     8461 gcgatctcgg cgtcctttac ctccatcagg cgacgggttt ccgccagctc gccgcgcagc
     8521 tcgtcgaccg cctcggccag cgccggcgac ggactggtgg catcgcggac cggatccgcg
     8581 gcgggcgccg acggcggcgt gtcggccggt ggcgccagtt cgagccggcg ctcggcgacc
     8641 ggcgcggccg cgccgcgcca ggcctcgttc tggcgacgca cctcggcggt cgcctcgccg
     8701 cccgaggtgg cggccatttc gctgcggctc ggcacccgca ggatggcgcc ggcgcgcagc
     8761 cggttgatgt tgccgtcgaa ggcgttcggg ttggcgcgga acagcgccac catgacctgg
     8821 ttggtgctca acccggtgtc ggggcgcacc cgctgcgcga tggcccacag cgtgtcgttg
     8881 cgctgcaccg gtccgaattc ggcgccgtac tcgctgcgca gcggcgtcgg tgcggcgctc
     8941 ggcgccgggg cgggcgcggg ccgcgcgacc ggcgcctggc gcggcgtgtc gaccgcggcc
     9001 ggcgatgcag gcgccgcctc gggcgccggc atgaaggtcg gcggatccag cagcacggtg
     9061 tactcgcgca gcatctggcc accgctccag gcggcctcca ccaggaaagt gacgaaaggc
     9121 tcggcgacgg ggcgggtgga ggtcacctgc accactgcgc tcgcggcccc ggtgcggcgc
     9181 acctggaagc gcaagtcgtc gaggaacgcg ggtcggtcca ggccgtagcg gctgaaggcg
     9241 gccgccggcg ccagccggat ctgcagcgac tcgagttccg ccggcgaggc gatgctgagg
     9301 gcgatctccg cattcatcgg ctgattgagg taggaattca ggcgaatgtc gcccagtccg
     9361 agggacaagg ccgcccctgg cgcgaggatg agggagagca ccccaagtag tatcagcttg
     9421 cgcgacattg tcactcctga ttgcgacccc gtgcgatcag ccacggggca cagctttagc
     9481 tatgcgccgc catcgtgaga aagcacttca cgacagcggc ctaactataa gctcacagat
     9541 atgtttttac aaaagtttca gcgatctgga cactgttcag cgccgcgccc ttgcgaatgt
     9601 tgtccgccac gatccagaga ctcaagccgc gcgggtggga aatgtcctcg cgaatgcgcc
     9661 ccacccacac ggcgtccgcg ccgctcgcct cggtcaccgc ggtcgggtag ccgccgggca
     9721 cgcgccggtc gagcacctcc acaccgggcg cctggcgcag cagccggcgg gcctcccggg
     9781 cgctgatctt ctcccgggtc tcgatgtgca ccgcctcgga gtgaccgaag aacaccggga
     9841 cccggacggc cgtcgcgttc acgccgatct cggggtcggc gaggatcttc ttcgtctccc
     9901 agcacatctt catctcttcg cgcgtgtagc cgttgtcctc gaacacgtcg atctgcggca
     9961 ggacgttgaa ggccagctgg cgcggcgtga gggtcttgcc caggggacgg ccgttcatca
    10021 gctgtgcggt ctggcgcgcc agctcgtcca ccatgcgttt gcccgccccg gagatcgact
    10081 ggtaggtcgc cacgttgatg cgcgtgatgc cgaccgcgtc gtagatcggc ttcagggcca
    10141 cgaccatctg gatggtcgaa cagttcgggt tggcgatgat gttgcgggcg aaggcctgct
    10201 cgagcgcgcc cgcattgacc tcgggcacca ccagcgggac gtcgtcgtcg tagcggaagc
    10261 acgaggtgtt gtcgatgacc atggcgccgg ccgcggcggc ccgcggggcg tgctccttcg
    10321 agaccgagcc gccggccgag aacaacgcga tgtcgacgcc ggaaaaatcg cagctcgcga
    10381 gctcggccac ccggtgggac ttgccgccga aatcgatcct ggtgccggcg gaacgctcgc
    10441 tggccagcag cttgagctcc gcgacgggaa acttgcgttc gcccaggcag gccagcatgg
    10501 cctcgcccac cagccccgtg gcgccgacga ccgcgaccga aaaacccttc ttcctgctca
    10561 tgcaccgtcc cgcatggttc atccatcccg gcgcgcgtcc gcgccgcccc agccccggtc
    10621 gaggccggcc tcgccggctc gcaggcccca ccaggccacc gccatcgcca ccgccgccac
    10681 gacggcggca gcgatccagg ccgcgtcggc gccgcccagc gaccaggcat agccgctggc
    10741 gagactgccg atcgcgccgc cgaccccgaa tccgaccgca ctgtacaacg cctgccccct
    10801 gccttgcagc cgtccggcaa aggcccggtg caccagcagc acggccaccg catggaacag
    10861 gccgaaggcc gccatgtgca gcgtctgggc gaacagcagc cacggcaggg aggcggcggc
    10921 ccaggcggtc aacaaccagc gcagcaccga cgccccgagc gcgaacacca gcaggccgcg
    10981 aaacccgaat cggccgatca gccgcggagt atagataaac atgagtatct cggcagccac
    11041 gccccaggcc cacagcaggc cggccatgcg ggtgccgtaa ccgagctcgt ccaggtagat
    11101 ggtgaagaac acgtagtagg gcccgaacga ggcctgttgc aggatgcaca cggcgagcag
    11161 cgccagcacc cacggccggc gcagggcggc cgcgaacgct cccgggacgt gcgctgccac
    11221 cggcgccggc gccggcggca cccgccagac ggccaccgcg gtgcctagca ccaccaggag
    11281 cagcgccacc ggcacggcac cgatctgctc gccccggaac agccacccgc cgagcagcac
    11341 ggccaggatg aaaccgaccg agccccacag ccggatatgg ccgtagcgct gcggccgccg
    11401 gccgagatgg ctcagggtgg tcgcctcgaa ctgcggcagc aggccgctcc acggcagggc
    11461 gaaggccgcc agcgccagcg ccgttgcgac gaagcccgtg gcgcccagca gcagggccgc
    11521 cccgccggcc gccagcagcg cgcccacccg caaagtgaga tcgcgccgcc cgctgcgatc
    11581 ggcggcccag ccccacagcc cgggcgccag cacgcgcatc gccatgggga tggccatcag
    11641 ctggccgatt gccaccgcgt cgaatccccg gtgttgcagg tagaaggaga agtacggcac
    11701 gaacacgccg atcgcgccga agtagaagag gtagaaggcg gacaaccgcg gatacaggcc
    11761 gcccgcggtg ccgtgctcgg ccagggtcgg ccgctcagcg cggggcatcg gggacgagcg
    11821 gggctcccgg cacgacgccc gcgttctggc cgcggtcgcg caacacgtga tccatcaaga
    11881 ccagcgc
//
LOCUS       DAOMPG010000050        11832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2338_length_11832_cov_4.505816, whole genome shotgun sequence.
ACCESSION   DAOMPG010000050 DAOMPG010000000
VERSION     DAOMPG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11832
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_11832_cov_4.505816"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PLS34_04235"
     CDS             <1..158
                     /locus_tag="PLS34_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HRP86712.1"
                     /translation="RVIWVTPKGAQGKRTAGIGVQFSEQDRGSTQRRIETYLAGALGG
                     DKPTHTM"
     gene            complement(217..1299)
                     /locus_tag="PLS34_04240"
     CDS             complement(217..1299)
                     /locus_tag="PLS34_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP86713.1"
                     /translation="MPGKSLDVAQLKAFSPLDGLKAENLRALAEKTTVQELPGGRFLF
                     RKGDTDRRTIYVISGEVELRDGDRMLSVIEGGTPDARSPLSPGLPRRVSARARSKIEF
                     IVIDSDLLDVMLTWDQTGTYEVSELRGDNLPESDDWMTTLLQTKAFHRIPAANIQAIF
                     MRMQQVNMKAGDVVIKQGDDGDFFYVITNGRCAVTRETPLNKDGIKLADLGPGDTFGE
                     EALISQAKRNATVTMITDGSLMRLGQEDFNTLMNEPMLQWVDYEQARKLILQGGKWLD
                     VRLPSEFESFHQEGAVNIPLYFIRLKLKSLDKNVRYVVCCDTGRRSSAAAYILSERGF
                     DAHVLKGGLTMADPEGERRKPAESGD"
     gene            complement(1444..3474)
                     /locus_tag="PLS34_04245"
     CDS             complement(1444..3474)
                     /locus_tag="PLS34_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRP86714.1"
                     /translation="MNKTFKHWNLRRDEDGIAWLCFDKADASTNVLSREVMVELNDQL
                     AALEKKLPKAVVIHSGKKSGFIAGADIKEFTGLRDADEAYEMVRSGQHVLDRLEALNC
                     PTIALINGFALGGGLELALACRYRIINDDPKTVIGLPEVKLGIHPGFGGTVRSIRLAG
                     PLKAMDMMLTGKNIRPKQARAMGLVDEVVPARHLERAARMMALKPPRPRQLPLKERLM
                     NAAPARRVLSGVLEKQVAKKARRDHYPAPYAIIDLWKDYGDKPALMMEKEARSIAALF
                     LSDTSRNLVRVFLLQDKLKALGGKSRHEFRHVHVVGAGVMGGDIAAWCALRGMTVSLQ
                     DREPKFIAPVIKRARKLYEKKLRDPRLVQAALDRLMPDVEGRGVARADVVIEAIFEDA
                     DAKKALFRTLEPQMKPDAVLATNTSSIRLEDLQDALDRPARLIGLHFFNPVAMMPLVE
                     IIHTPDTPRAEIDKGIAFARKIDKLPLPCLSSPGFVVNRVLMPYMMEALLAGEDGVPM
                     PLIDEAAKRFGMPMGPVELADTVGLDVALHVARILGTAYGIPVPDKLIEMVEKKRLGR
                     KTGRGFYDWKDGKAVKPAVKDAKAPQDLADRLVLPMVNEAVAVWRDGVVTDLDLLDAG
                     IIFGTGFAPFRGGPINFARARGVADVVDTLKRLEDRYGERFEPDPGWQDIAA"
     gene            complement(3479..4789)
                     /locus_tag="PLS34_04250"
     CDS             complement(3479..4789)
                     /locus_tag="PLS34_04250"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRP86715.1"
                     /translation="MAKTRHASYASPAYVVDGARTPFLKARGRPGPFSASDLAVAAGR
                     ALLARQPIAPEQLDEVILGCAMASPDEANIGRIVALRLGCGNRVPGFTVMRNCASAMQ
                     ALDSAARNIWLGKADLVLAGGTEAMSRAPVLFNEEMVNWLADLARAKAPLDKALHIAR
                     LRPKHFAPVIALIKGLTDPVVGLNMGQTAEIISDRFGISRKEMDRYSVQSHLRLAAAF
                     DEGRMDEVVAIYDPAGRAHEEDDGMRRDSNEEKLATLKPFFDRRVGQVTAGNSSQITD
                     GAALLLLASEAAVKKHKLEVLGTIEDCEWAALDPAEMGLGPAHSSAPLMQRHGLGLDD
                     VDYWEINEAFAGQVLACLAALDDADYCREHFGLDGAVGRIDEAKLNIDGGAIAAGHPV
                     GASGARIVLHALKVLERQGGGTAIATLCIGGGQGGAMLLKSKPE"
     gene            complement(4799..5491)
                     /locus_tag="PLS34_04255"
     CDS             complement(4799..5491)
                     /locus_tag="PLS34_04255"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP86716.1"
                     /translation="MTSLLDSTKDRILAAAEELFARTGVARTSLRAITALARVNLAAV
                     NYHFGSKEGLVEEVYRRRLEPLNRARLLNLERLERRARKPSLEDVLEAFMSPVAQLAR
                     DPAQGGPTVMRLLGQTHAEAEEPFKAWFAGEYKRVLERYHAALCRTLPGIPPEDVRWR
                     LQFLVGALTYPVAERQLVELVSGEAMDPADVNQVVERLLPFVAAGFRVPPGTPKPARR
                     ATRRRPKVTRNS"
     gene            complement(5596..6330)
                     /locus_tag="PLS34_04260"
     CDS             complement(5596..6330)
                     /locus_tag="PLS34_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRP86717.1"
                     /translation="MKFLLYNIRYAAGVGAKFNLPVPGAGYLRGNTDNLNAITQFIKS
                     HSPDVVGLVEVDTGSIRTRMVNQAEAIARQLGHYSTYQCKYGESSFNNRLPIVRKQSN
                     AFLAAPRVHGERFHYFDTGIKRLIIELELEDVAIFLVHLSIKYRHRHWQLRHLHDLVV
                     KSSKPVIVAGDFNTFWGENEMYLFMQATGLRSANVAGLPSYPSTAPRKELDFILYGSG
                     LRPTAFEIPQVRFSDHLPLICEFEHE"
     gene            6489..6941
                     /locus_tag="PLS34_04265"
     CDS             6489..6941
                     /locus_tag="PLS34_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86718.1"
                     /translation="MSESNPIRVYAVHGWEKDEDYVRLFEYLESADNFFYRAISNPDA
                     HPPEGDGVAARRTQIGALLKDAECVVCPAGTWERHNDWARYTVETAHALQLPIVAIEH
                     FGPKNIDIRLKGHANATVGWDSRSIVDAIRREARQEDTTRFDTIEFDM"
     gene            complement(6984..7667)
                     /locus_tag="PLS34_04270"
     CDS             complement(6984..7667)
                     /locus_tag="PLS34_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86719.1"
                     /translation="MNRRQHFRRMLGAAVAAVTLIGGLAVAADAWREIPYSRLHEAFT
                     TIQPLDDARYIQLRHTVGRADADTADEPLRLVIASASGDIEVPVNTDGTVDFPLSQAL
                     LEEDPPVRTNAPEGGLSVGMTIDISAPPTERFPYALLADIEDEYSRMVRKQGLMARML
                     APRPAGLEVRFAAGEPGTATVGGRNGRVFQADDQGRLRIPSRREWRRENPEVVLSHMP
                     ELISLAVGD"
     gene            complement(7696..9183)
                     /locus_tag="PLS34_04275"
     CDS             complement(7696..9183)
                     /locus_tag="PLS34_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRP86720.1"
                     /translation="MPPAQPSSRAPALLAELRALRRDVEHGAAEILANWESRIERPGF
                     QEPAHNLAQYIVLRRMDLRPLQDELRLFGLSTLGRVEGRVMANLDAVIAALAAIAGDP
                     EPCPFPDAAIMRHGPEAIRAGADRALGPCLGPRTVRLMVTLPAEVGEDEGLALSLVEN
                     GADLVRINCAHDGPKQWQAMIANVRQAARSIGRPVRVLMDLGGPKCRTGVVIAAKGHK
                     RLCSGDSFLLLRPDVEVRTDWPVQAGCTLASVFDNLRPGAPVWVDEGRLGGVVRTLLP
                     EGAVVDVRSAPPRGLKLKADKGLNFPDTDLGVPPLTDKDLADLDFVAANADLVGYSFV
                     QEAGDIERLQAELALRAGERRAAAIGIVAKIETPRAVRNLPEIIVQGAGRQPFAIMIA
                     RGDLGVEIGFGRLAEIQEEILWLCEAAHVPVIWATGVLNGLIKDGQPNRGEMTDAAMS
                     ARAECVMLNKGPHVVAALRCLDELFVRMAEHQQKKSPRLRALKSW"
     gene            complement(9334..11154)
                     /locus_tag="PLS34_04280"
     CDS             complement(9334..11154)
                     /locus_tag="PLS34_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRP86721.1"
                     /translation="MTVEIAIVFLVVAGALYLFASEALPLDITAILILITVMVIPLLG
                     HSQWLLDRGIDLQAAFPTVSEGLSGLSSTATVTVLAMFILSAGIQRSGLIHVLGKAVF
                     PLVGNSELRMILIIAALVGGVSGFINNTAAVAVAIPLVLDMAKRLKMLASRLLMPVSF
                     FGMLGGTLTLIGTSTNILASAIMKDQPEFGRQIGMFEFTHLGLIVLGVGLLYFVTIGR
                     LLLPKTDRVKIQGEDDETFIIELGVRDESELVGKTLEENRFAARAGVEVLKLVRGQTA
                     HVKRAEDTKIKAGDVLQVRGNLRQVMDLIKSEEVEVLSEMGGAQQVRADGQLVRVLLR
                     DRQVFKGKTAADVDFWKRYQARLIGLETDMVQSRRLADEKLHVGEIALLQLSDTALVS
                     LRRRHDLVLLEEAKDDFDRRRMWIAGGIVAGVVLGAALTPMPIVVTALLGIVAMVATG
                     CVKKDDMYTSVSWDVIFLLAGVIPLGIAMTKSGAADWLGGLLAMHASGWHPILVLMAL
                     YAVTTLLTELVSNNASVVILVPVAISLGQLLQIDILPLVLAVMFAASTSFLSPIGYQT
                     NTMIFGTGLYKFTDFAKVGAPLNAILMVVTSVSIHLLWIA"
     gene            11249..>11832
                     /locus_tag="PLS34_04285"
     CDS             11249..>11832
                     /locus_tag="PLS34_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRP86722.1"
                     /translation="MNTTRRDFLKLGGLGAAALASGSILAGSNSAFAATEATGRPLRM
                     LILGGTGFTGPHQVRYALERGHQVTLFNRGRRSIDWPAAVEELTGDRDSGDLEALRGR
                     QWDVCIDNPTSVPAWVRDAGEVLAGNIGHYVFISTLSVLAETSRPGQAEDAPLAEYTG
                     ADAMQETMASLRADMRLFGPLKAACEAEAERRFPG"
BASE COUNT         1929 a         3984 c         3965 g         1954 t
ORIGIN      
        1 ggcgcgtcat ctgggtgacg ccgaagggcg cccagggcaa gcgcaccgcc ggcatcggcg
       61 tgcagttcag cgagcaggac cgcggcagca cccagcgccg catcgaaacc tacctggccg
      121 gcgcgctcgg cggggacaag ccgacccata ccatgtaggc ggtgcgccgc ggccggcgca
      181 agatcagccg atgcgagcga agtcgcgtgg cgactctcag tcgccggact cggccggctt
      241 gcgacgttcg ccctcggggt cggccatcgt caggccgccc ttgagcacgt gcgcgtcgaa
      301 gccgcgctcg ctgaggatgt aggcggccgc gctgctgcgc cgacccgtgt cgcagcacac
      361 gacatagcga acgttcttgt ccaggctctt caatttcagc cggatgaagt agagcgggat
      421 gttgacggcg ccttcctggt ggaagctctc gaattccgag ggcaggcgga cgtccagcca
      481 cttgccgccc tgcaggatca gcttgcgcgc ctgttcgtag tccacccact ggagcatcgg
      541 ctcgttcatc agggtgttga agtcttcctg gccgaggcgc atgagcgagc cgtcggtgat
      601 catggtcacc gtggcgttgc gcttggcctg cgagatgagg gcctcctcgc cgaaggtgtc
      661 gcccggaccg agatccgcca gcttgatgcc gtccttgtta agcggcgtct cgcgggtcac
      721 cgcacagcgg ccgttggtga tgacatagaa aaagtcgccg tcatcaccct gcttgatgac
      781 gacgtcgccg gccttcatgt tgacctgctg catgcgcatg aaaatggcct ggatgttcgc
      841 cgccggaatg cggtggaagg ccttggtctg gagcagcgtg gtcatccagt cgtcgctctc
      901 ggggaggttg tcgccgcgca gttcgctgac ctcgtaggta ccggtctggt cccaggtcag
      961 catcacgtcg agcaggtcag agtcgatgac gatgaattcg atcttgctgc gtgcccgggc
     1021 gctcacccgg cgcggcaggc cgggcgacag ggggctgcgc gcatccgggg tgccgccttc
     1081 gatgacggac aacatgcgat cgccgtcgcg cagctcgact tcaccggata tgacataaat
     1141 cgtgcggcga tccgtgtcgc ccttgcgaaa caggaagcgc ccgccgggca attcctgcac
     1201 ggtcgttttc tcggcgagcg cccgcaggtt ctcggccttg agaccatcca gcggcgaaaa
     1261 cgccttcaac tgggcgacgt ctagggactt accaggcatg gcgggggatc ttgctccctt
     1321 tcttgtcgtg tctgcttccg cgtcgggcgt ttggcggcgc ggtacgtgac gctccgccaa
     1381 ctggggagaa tatcacagga aacgccgtcg tcaggggtcc cacgggccga tcatgacgat
     1441 ccgtcaggcg gcgatatcct gccagcccgg atccggctcg aagcgttccc cgtagcggtc
     1501 ctcgagccgc ttcaaggtgt ccacgacatc cgccacgccg cgcgcgcggg cgaagttgat
     1561 cgggccgccc cggaacggcg caaagccagt gccgaagatg atgcctgcgt ccagcaggtc
     1621 caggtcggtc accacgccgt cgcgccagac cgcgactgct tcgttgacca tcggcagcac
     1681 cagtcggtcg gcgagatcct gcggcgcctt cgcgtccttg accgctggct tgacggcctt
     1741 gccgtccttc cagtcgtaga agccgcgtcc cgtcttccgg ccgagacgct tcttctcgac
     1801 catctcgatc agcttgtcgg gcaccgggat gccgtaggcg gtgccgagga tcctggccac
     1861 gtgcagggcg acgtcgaggc cgacggtgtc ggccagctcg accggtccca tcggcatgcc
     1921 gaagcgcttg gccgcttcgt cgatcagcgg catgggcacg ccgtcctcgc cggccagcag
     1981 ggcctccatc atgtacggca tcagcacgcg gttgacgacg aaacccggcg aactcaggca
     2041 gggcaggggc agcttgtcga tcttgcgtgc gaaggcgatg cccttgtcga tctcggcgcg
     2101 cggggtatcc ggcgtatgga tgatctcgac cagcggcatc atcgcgaccg ggttgaagaa
     2161 atgcagcccg atcagccgtg ccggcctgtc cagggcgtcc tgcaagtcct cgagccggat
     2221 gctcgaggta ttggtggcca gcaccgcgtc gggcttcatc tgcggctcga gcgtgcggaa
     2281 cagcgccttc ttggcgtcgg cgtcctcgaa gatcgcctcg atgacgacgt cggcacgggc
     2341 cacgccgcgg ccctcgacgt ccggcatcag ccggtcaaga gccgcctgca ccaggcgcgg
     2401 atcgcggagc ttcttctcgt acagcttgcg ggcgcgcttg atgaccggcg cgatgaactt
     2461 gggctcccgg tcctgcaagc tcacggtcat gccgcgcagc gcgcaccagg cggcgatatc
     2521 gccacccatc acgccggcgc cgaccacgtg gacgtggcgg aactcgtggc gactcttgcc
     2581 gccgagggcc ttgagcttgt cctgcagcag gaacacgcgc accaggttgc gggaggtgtc
     2641 gctgaggaat agcgccgcaa tcgaacgcgc ttccttttcc atcatcaggg cgggcttgtc
     2701 gccgtagtcc ttccacaggt cgatgatggc gtagggcgcg ggatagtggt cgcgccgcgc
     2761 ttttttcgcc acctgcttct cgagcacgcc cgacagcacc cggcgcgccg gcgctgcatt
     2821 catcagccgc tccttgagcg gcagctgacg cggcctgggc ggcttgagtg ccatcatgcg
     2881 cgccgcacgc tccaggtgcc gcgccgggac gacctcgtcg accagcccca tggcacgcgc
     2941 ctgtttcggg cggatgttct tgccggtgag catcatgtcc atcgccttga gcggtcccgc
     3001 caggcggatg gaacgcaccg tgccgccgaa gcccgggtgg atgccgagct tcacttccgg
     3061 cagcccgatc accgtcttcg ggtcgtcgtt gatgatgcgg tagcggcacg ccagcgccag
     3121 ttctagcccg ccgccgagcg cgaagccgtt gatcagcgcg atggtggggc agttcagggc
     3181 ctcgagccgg tcgagaacgt gctgcccgga gcgcaccatc tcgtaggcct cgtcggcgtc
     3241 cctgagaccg gtgaattcct tgatgtcagc gccggcgatg aagcccgatt tcttgccgga
     3301 gtggatcacc acggccttgg gcagcttctt ttcgagggcc gccagctggt cgttcagctc
     3361 gaccatcacc tcgcgcgaca gcacgttggt cgaggcatca gccttgtcga agcagagcca
     3421 cgcgatgccg tcctcgtcgc gacgcaggtt ccagtgcttg aaagtcttgt tcatgaggct
     3481 actccggctt gctcttcagc agcatggcgc cgccctggcc gccgccgatg caaagggtgg
     3541 cgatggcggt gccgccgccc tggcgctcga gcaccttgag tgcgtgcagg acgatgcgcg
     3601 cgccgctggc gcccacggga tggccggcgg cgatggcgcc gccgtcgata ttcagcttcg
     3661 cctcgtcgat gcggccgacg gcgccgtcga ggccgaagtg ctcgcgacag taatcggcgt
     3721 cgtcgagcgc tgccaggcag gccagcacct ggccggcaaa ggcctcgttg atctcccagt
     3781 aatccacgtc gtccaggccc agcccgtggc gttgcatgag cggggccgac gagtgcgcgg
     3841 gcccgagccc catctcggcg gggtcaagcg cggcccactc gcagtcttcg atcgtgccga
     3901 gcacctcgag cttgtgcttc ttcaccgccg cctcgctggc caacagcagc agcgccgcac
     3961 cgtcggtgat ctgcgagctg ttgcccgcgg tcacctggcc gacgcgccgg tcgaagaagg
     4021 gcttcagcgt ggccagcttc tcctcgttgc tgtcgcgacg catgccgtcg tcttcttcat
     4081 gggcccggcc ggccgggtcg tagatcgcca cgacctcgtc catccgaccc tcgtcaaatg
     4141 ccgccgccag ccgcaggtgg ctctgcaccg agtagcggtc catctccttg cggctgatgc
     4201 cgaagcggtc cgagatgatc tcggcggtct ggcccatgtt gaggccgacc accgggtcgg
     4261 tcaggccctt gatgagtgcg atcacgggcg cgaagtgctt cggccgcagg cgcgcgatgt
     4321 gcagcgcctt gtcgagcggc gccttggcgc gcgccaggtc ggcgagccag ttcaccattt
     4381 cctcgttgaa cagcaccggc gcgcgactca tggcctcggt gccgccggcc agcaccaggt
     4441 cggccttgcc gagccagatg ttgcgcgctg ccgagtccag cgcctgcatt gccgaggcac
     4501 aattgcgcat caccgtgaag ccgggcacgc ggttgccgca gccgagccgc agcgccacga
     4561 tccgcccgat gttcgcctcg tccgggctcg ccatggcaca gccgagaatg acctcgtcga
     4621 gctgttcggg ggcgatcggc tggcgtgcca gcagggcccg gccggccgca acggccaggt
     4681 cggaggcgct gaagggcccc ggccggccgc gggctttcag gaagggcgtc cgcgcgccgt
     4741 ccaccacgta ggcggggctc gcgtaagacg cgtggcgggt cttggccatg atctgctcct
     4801 aactgttcct ggtaactttc ggccgccggc gcgtggcgcg gcgagccggt ttcggggttc
     4861 cgggcgggac gcggaaacct gccgcaacga agggcagcag gcgctccacc acctggttga
     4921 cgtcggccgg gtccatggcc tcgccggaca cgagttcgac gagctggcgc tccgcaaccg
     4981 ggtaggtcag cgcgccgacg aggaactgca gccgccaacg gacgtcctcg ggcggaatac
     5041 cgggcagggt ccggcacagc gccgcatggt agcgttccag cacgcgcttg tactcgccgg
     5101 cgaaccacgc cttgaacggt tcctcggcct cggcgtgcgt ctggccgagc aggcgcatca
     5161 ccgtcggacc gccctgggcc gggtcgcgcg ccagctgcgc caccgggctc atgaaagcct
     5221 cgagtacgtc ctcgagcgac ggcttgcggg cgcggcgctc gagccgctcc aggttgagga
     5281 ggcgggcacg attcaggggc tccaggcggc gacggtagac ctcttcgacc aggccttcct
     5341 tggagccgaa atggtagttc acggccgcca ggttgacccg tgccagggcg gtgatggcgc
     5401 gcagcgaggt gcgggccacg ccggtccggg caaacagctc ttcagccgcc gccaggatcc
     5461 ggtccttggt ggaatccagc agactcgtca aaccgggatg cctgtttgaa acaaccgttt
     5521 aatggtaggc gcaggccggc gccaccgcaa caaagcccgg tcggcctggc cgacccgggc
     5581 ggccaggcca ggccgctact cgtgctcgaa ttcgcagatc agcggcaggt ggtcggaaaa
     5641 gcgcacctgg ggaatctcga aggccgtggg tcgcaatccc gacccgtaga gaatgaaatc
     5701 gagctccttg cgcggcgccg tgcttgggta ggacggcagg cccgcgacgt tggcgctgcg
     5761 cagcccggtg gcctgcatga acaggtacat ctcgttctcg ccccagaaag tattgaagtc
     5821 gccggcgacg atgaccggct tgctggactt gaccaccagg tcgtgcaggt gccgcagctg
     5881 ccaatggcgg tggcggtact tgatcgacag gtgcaccagg aaaatggcca cgtcctcgag
     5941 ttcgagctcg atgatcaggc gcttgatgcc ggtatcgaaa tagtggaaac gctcgccgtg
     6001 cacgcgcggc gctgcgagga aggcattgga ctgcttgcgc acgatcggca ggcggttgtt
     6061 gaaggagctt tcgccgtact tgcactggta ggtgctgtag tggcctagct gccgggcgat
     6121 ggcctcggcc tggttgacca tgcgcgtgcg gatcgagccg gtatccacct cgaccagtcc
     6181 gaccacgtcc gggctgtggg acttgatgaa ctgcgtgatg gcgttgagat tgtcggtgtt
     6241 gccacgcagg tagccggcgc ccgggaccgg gaggttgaac ttcgccccca cgccggcggc
     6301 gtagcgaatg ttgtagagca ggaacttcac gccgccgccg gacaggtgct tcgatctgac
     6361 atcagcaaat tctaaccaac gcggggccct cgtgccgcac ccggctcctg ccaggcctgc
     6421 acttggactc cttcgcccga gaccgcatac tgcggcgaag gtagtggaac cgggacgagg
     6481 agggcgctat gtcggagagc aacccgatca gggtctatgc cgtgcatggc tgggaaaaag
     6541 acgaggacta cgtgcggctg ttcgagtacc tggagagcgc cgacaacttt ttctaccggg
     6601 cgatctccaa cccggacgcc cacccgccgg agggcgatgg cgtagcggcg cgccggacgc
     6661 agatcggcgc cttgctcaag gacgccgaat gcgtggtctg cccggcgggc acctgggagc
     6721 gccacaacga ctgggcgcgg tacacggttg agaccgctca tgccctccaa cttccgatag
     6781 tcgcgattga gcatttcggt ccaaaaaaca ttgatatacg actgaaaggt catgcaaatg
     6841 ctacggtggg ctgggactcc cgcagcatcg tcgacgcgat tcgtcgcgag gcgcgccagg
     6901 aggacacaac gcgcttcgac accatcgagt tcgacatgta gcaacggcgt ccgggtcatc
     6961 cagcgaccac ccgagcagcg ggttcagtcg ccgaccgcca gactgatgag ttccggcatg
     7021 tgcgacagca ccacctcggg attctcccgg cgccactcgc gacgcgacgg aattcgcaac
     7081 cggccctggt cgtccgcctg gaacacgcgg ccgttgcgcc cgcccacggt cgcagtgccc
     7141 ggctcgccgg ccgcaaagcg gacttccagc ccggcaggcc tgggcgccag catgcgcgcc
     7201 atgaggccct gcttcctgac catccgggag tactcgtcct cgatatcggc aagcagcgca
     7261 tagggaaagc gctccgtcgg cggcgccgag atgtcgatgg tcatgccgac cgacaggccg
     7321 ccctcgggcg cattggtgcg taccggcgga tcctcctcca gcaaggcctg gctgagcggg
     7381 aaatccaccg tgccatccgt gttgaccggc acctcgatgt ctcccgatgc gctggcgatg
     7441 accagccgca acggctcgtc ggcggtgtcg gcgtccgcgc ggccgacggt gtggcgcagc
     7501 tggatgtagc gcgcatcgtc gagcggttgg atggtggtga acgcctcgtg cagcctggaa
     7561 taggggattt ctcgccaggc gtcggcggcg accgccagtc cgccgatcaa cgtgacggcc
     7621 gccacggccg caccgagcat gcgccggaag tgttgcctgc gattcatctg cggaaacccc
     7681 tgctgggcgg aacgcctacc aggattttaa cgcacgcagc cgcggtgatt tcttctgctg
     7741 gtgttcggcc atgcggacga agagttcgtc gagacagcgc aatgccgcca cgacgtgcgg
     7801 tcccttgttg agcatgacgc attcggcgcg cgccgacatc gccgcatcgg tcatctcgcc
     7861 ccggttgggc tggccgtcct tgatcaggcc gttgaggacg cccgtcgccc agatcaccgg
     7921 cacgtgagcc gcttcgcaca gccacagtat ctcttcctgg atctccgcga ggcgcccgaa
     7981 gccgatctcg accccgaggt cgccgcgcgc aatcatgatg gcgaacggct gcctaccagc
     8041 cccctggacg atgatctccg gcaggttgcg cactgcccgc ggtgtctcga tcttcgccac
     8101 gatgccgatg gccgccgccc gtcgttcgcc ggcccgaagg gccaactcag cctgcagccg
     8161 ctcgatgtcg cctgcttcct ggacgaagga gtagccgacc aggtcggcat tcgccgccac
     8221 gaaatccagg tcggcgaggt ccttgtcagt cagcggcggg acccccaggt ccgtgtcggg
     8281 gaaattcagt cccttgtccg ccttcagctt gaggccgcgc ggcggtgcgc tgcgcacgtc
     8341 cacgacagca ccctcgggga gcagggtacg caccacgccg ccgagccggc cttcatccac
     8401 ccagaccggc gctccggggc gcagattgtc gaacacgctg gccaaggtac atccggcctg
     8461 caccggccag tccgtgcgta cctcgacgtc cggacgcagg agcaggaaac tgtctccgct
     8521 gcacagccgc ttgtgacctt tggcagcgat gacgacgccg gtacggcact tcggtccacc
     8581 caggtccatc agcacgcgga ccggcctgcc gatcgaccgg gcggcctgcc tgacgttcgc
     8641 gatcatggcc tgccactgct tcggtccgtc gtgggcgcag ttgattcgca ccaggtcggc
     8701 gccattctcc accagcgaca gggccaggcc ctcgtcttcg ccgacctccg ctgggagcgt
     8761 caccatcagg cggaccgtgc gcggccccag gcagggaccg agcgcgcggt ctgcgccggc
     8821 acgaatggcc tcgggcccgt ggcgcatgat ggcggcgtcg ggaaacgggc acggctcggg
     8881 atcgccggcg atggccgcca gggccgcgat cacggcgtcc aggttggcca tgacgcgccc
     8941 ctcgacgcga cccagcgtgg acagcccgaa cagcctcagc tcatcctgaa gcggccgcag
     9001 gtccatgcgc cgcagcacga tgtactgtgc caggttgtgg gcaggctcct ggaagccggg
     9061 gcgctcgatg cgcgactccc agttggccag gatttccgcc gcgccgtgct cgacgtccct
     9121 gcgcaatgcg cgcaactcgg ccagcagcgc cggagcgcgc gaggacggct gcgcaggcgg
     9181 caaggaccct gccgggcaat gggatacttg gagggtcatc ccatcaggct aggggcggca
     9241 ggttgcatcg acattacggc cgtgtgacat gtgccggggg gagggcggcg gcccgccggc
     9301 accggcccga acccgggacc gcaggcctca gcctcaggcg atccagagca ggtggatgct
     9361 gacgctggtg accaccatca ggatggcgtt cagcggcgca ccgaccttcg cgaagtccgt
     9421 gaacttgtac aggccggtgc cgaaaatcat ggtgttggtc tggtagccga tcggcgaaag
     9481 gaaactggtc gaggcggcga acatcaccgc cagcaccagc ggcaggatgt cgatctgcaa
     9541 caactggccg agcgagatcg ccaccggcac caggatcacc accgaggcat tgttgctgac
     9601 cagctcggtc agcagggtgg tcaccgcgta cagcgccatc agcaccagga tggggtgcca
     9661 gccggaggca tgcatcgcca gcaggccgcc gagccagtcc gcagcgcccg acttggtcat
     9721 ggcgatgccc agcgggatca cgcccgccag caggaagatc acgtcccagg acacgctggt
     9781 gtacatgtcg tccttcttca cgcagccggt cgctaccatg gcgacgatgc cgagcagcgc
     9841 cgtgaccacg atcggcatcg gggtcagcgc ggcgccgagc acgacgccgg cgacgatgcc
     9901 ccctgcgatc cacatgcgcc gccggtcgaa gtcatccttg gcttcctcca gcagcaccag
     9961 gtcgtggcgc cgccgcaggc tcaccagcgc ggtgtcggac agttgcagca gggcgatctc
    10021 gccgacatgc agtttctcgt ccgccagccg acggctctgc accatgtccg tctccagccc
    10081 gatcagccgc gcctggtagc gcttccagaa gtccacgtca gccgcggtct tgcccttgaa
    10141 cacctggcga tcccgcagca atacccgcac cagctggccg tcggcgcgca cctgctgtgc
    10201 cccgcccatc tcggacaaca cctcgacttc ctcggacttt atgaggtcca tgacctggcg
    10261 caggttgccg cgcacctgca gcacgtcgcc ggccttgatt ttcgtgtcct cggcacgctt
    10321 gacgtgcgcg gtctggccgc gcaccagttt cagcacctcg acgccggcgc gcgccgcgaa
    10381 cctgttttcc tcgagcgtct tgccgaccag ttcgctctcg tcccggaccc cgagctcgat
    10441 gatgaaagtc tcgtcgtctt cgccctggat cttgacgcgg tccgttttcg gcagcagcag
    10501 ccggccgatg gtgacgaaat acagcaggcc gacgccgagt acgatcagac cgagatgggt
    10561 gaattcgaac atgccgatct ggcgaccgaa ttccggctgg tccttcatga tcgccgaggc
    10621 caggatgttg gtcgacgtgc cgatgagggt gagcgtgccg ccgagcatac cgaaaaagct
    10681 gaccggcatg agcagccgcg aggccagcat cttcagccgt ttcgccatgt cgagcaccag
    10741 cggaatggcc acggccacgg cggcggtgtt gttgatgaag ccggaaacgc cacccaccag
    10801 cgccgcgatg atcaggatca tgcgcagctc cgagttgccc accagcggga agaccgcctt
    10861 gcccagcacg tggatcagcc ccgaacgctg aatgccggcc gagagaatga acatcgccag
    10921 cacggtcacg gttgccgtgc tggacaagcc ggacaggccc tcgctgacgg tcggaaaggc
    10981 ggcctgcagg tcgatgcccc ggtccagcag ccactgggaa tggcccagca gcggaatgac
    11041 catgaccgtg atgagaatca ggatggcggt gatgtcgagc ggcagcgcct ccgaggcgaa
    11101 caggtacagt gccccggcga cgaccaggaa gacgatggcg atttcgacgg tcatcgggtg
    11161 ctagtgtaat cgctcggcag gaggtgcggg cgccgccgcg gccgcaatct gtgtgacact
    11221 ggcgcgccgg atgcagcgag gacgagccat gaacacgacg cgacgggatt ttctcaagct
    11281 cggcgggctg ggcgccgcag cgcttgcaag cggctcgatc ctggcgggct cgaacagcgc
    11341 gtttgcggcc accgaggcga ccggccgccc gctgcgcatg ctcatcctcg gcggtaccgg
    11401 cttcaccggc ccgcaccagg tgcgctacgc gctcgagcgc ggccaccagg tcacgctgtt
    11461 caaccgcggc cggcgcagca tcgactggcc ggctgcagtc gaggaactca ccggcgaccg
    11521 cgacagcggc gacctcgaag cgctgcgcgg ccgccaatgg gacgtctgca tcgacaatcc
    11581 caccagcgta ccggcctggg tgcgggacgc cggcgaggtg ctggccggca acatcggcca
    11641 ttacgtcttc atctcgaccc tgtccgtgct ggccgagacc agccgcccgg gccaggccga
    11701 ggatgcgcca ctggccgagt acaccggtgc ggacgccatg caggagacca tggcgtcgct
    11761 gcgcgccgac atgcgcttgt tcggtccgct gaaggcggct tgcgaggctg aagccgaacg
    11821 ccgcttcccc gg
//
LOCUS       DAOMPG010000051        11685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2369_length_11685_cov_4.043938, whole genome shotgun sequence.
ACCESSION   DAOMPG010000051 DAOMPG010000000
VERSION     DAOMPG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11685
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_11685_cov_4.043938"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1934)
                     /locus_tag="PLS34_04290"
     CDS             complement(123..1934)
                     /locus_tag="PLS34_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRP86723.1"
                     /translation="MATTRNPDGSETVSDGVDRPRSRDLRPLRDLAPFLKPYRGMLLA
                     ALGALLLAAAATLSLPVAVRFMIDEGFSAANAANVDRYFLAMFGIASLLAISTSLRYY
                     LVSWLGERVVADVREAVFRHILRLSQSFYETTRTGEVLSRLTTDTTILQSVVGSSASI
                     ALRNMLLLAGGLVMLGITSPKLTGLIIVLVPIMLLPLILFGRRVRLLSRRNQDRIADS
                     SALAEEILTAMRIVQAYVREAWEAARFKDAVEDSFSAALARIRARALLTAVVILFVFG
                     SIVLVLWFGAQAVLGGTMTTGQLGQFVLYAVLVAGSFAALAEVWGEVQRAAGAMERIM
                     ELLTTTPDIQSPAAPLPAARPARGEVRLEQLSFSYPARPERRALDDVSFTVPAGKTVA
                     LVGPSGAGKTTVFQLLLRFYDPQSGRILFDGMDTAQLALADLRGAMAMVPQETVIFST
                     SARENIRYGRPEAGDAEVEAAARAALAHEFICDMPEGYDTYLGERGVRLSGGQRQRIA
                     IARAILKNPPLLLLDEATSALDAESERLVQQALDRLMTERTTIVIAHRLATVLKADRI
                     VVLEAGRVVAVGTHEELVAQEGLYARLARLQFAETTA"
     gene            complement(2007..2759)
                     /locus_tag="PLS34_04295"
     CDS             complement(2007..2759)
                     /locus_tag="PLS34_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRP86724.1"
                     /translation="MTDNPTLVAHRGLAATWPENTLPALQAAVAAGARWVEIDVQLSA
                     DGVPVLLHDADLVRVAGRPEAVFDLPAAALADIPVGEPARFGPRFPEARAPTLARFAQ
                     WLAGTEGVCAFVELKSQSIERFGRNAVADACLHAMTPAQGRWFPIASDYEILALAGDL
                     GAPELGWIVRGFDPAIETRARALPARWLFCNHERLAPGPLPRGAWEWVLYEVADATLA
                     RGLLARGARWLETMAIDELHDALQLGPAAGAP"
     gene            2834..4087
                     /locus_tag="PLS34_04300"
     CDS             2834..4087
                     /locus_tag="PLS34_04300"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRP86725.1"
                     /translation="MSFVSHLRGIGSGACYPADAVMNLCPVDGRPVEMVLDLERLRAA
                     RPGTSWLLPGRRDMWRFGALLPLDAGDPEEARHIVTLGEGATPLLDASAWPLARQGGF
                     ELLVKEEGRHVPGCGANPTHSFKDRGMAMVATQARRLGLRRLAVPTQGNAGDSLAEYA
                     RAAGLEVAVVMPGDTPRGVLDRVASLAAADPRIRLELVEGTIREAGARVKSHWVPAGW
                     FSVATFQEPGWRIEGKKTLGLELAEPVQPGGAWRLPDVIVYPTGGGTGIIGMWKAFAE
                     LEALGVIGPVRPRMVAVQSEATAPLVAALAAGADDTCAGTAGATLAVGLNVPGGVGHF
                     RVLEILRASGGGALAVADADMRDAFRAARHTLGMDLAPEGAACMAALPRLLDERLVRT
                     GDRVVVVNTCAPEKYLPELRDWYEA"
     gene            complement(4091..4762)
                     /locus_tag="PLS34_04305"
     CDS             complement(4091..4762)
                     /locus_tag="PLS34_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86726.1"
                     /translation="MANCLVTGANRGIGLALCTSLAERGDTVIAACRTSSSELKALGV
                     RIVDGIDVGDPASAQRLARAVEGESLDWLINNAGVLRRDTLGGLKGEALDDVYLQFRV
                     NSVGPLIISQALLGNLHQGSKIGIVTSRMGSMEDNTSGGYYGYRMSKAAVNAAGRSLA
                     MDLKERGIAVALLHPGYVRTEMVGGGGDVEPEDAAAGLIARMDELDITTTGGFWHAKG
                     QPLPW"
     gene            4837..5361
                     /locus_tag="PLS34_04310"
     CDS             4837..5361
                     /locus_tag="PLS34_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86727.1"
                     /translation="MANLRIAWLLVLLLATGCATPRAPTGLAMPAPDEAGAATLLAGY
                     LLAQGWTVRLAGEAAVAAQRGAEQLLLEPLLDPQGLDRILVSRTWPRAAQADDDALGA
                     FARELNEMLNVGQFRVSPDGLVLQSSLPFLDAVEPRLLNAFLAFTADVHYALLQVQGE
                     RQLLAAVEGDAASR"
     gene            5399..5896
                     /locus_tag="PLS34_04315"
     CDS             5399..5896
                     /locus_tag="PLS34_04315"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HRP86728.1"
                     /translation="MSVTVRARHEYRHPVSKVWAAFSDPEFYQAKFEGIGHRNVEVVA
                     TESDDEGFSIEVSREVPLDVPGFLRSMLGEWNTLLQNERWSEAGKGVYTNELEIEARG
                     VPAAMTGTMKLTSKGKGCVNEVAITIRAAVPLIGGKLEQFVAKDTEATLEAEYEFIQD
                     YLDNA"
     gene            complement(5934..6953)
                     /locus_tag="PLS34_04320"
     CDS             complement(5934..6953)
                     /locus_tag="PLS34_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HRP86729.1"
                     /translation="MNLKDSGFRNRVPGWRNRRALARTGGAAGAVLLLLLLGLSWYWS
                     REPAVFWVDWEKDGRPAVTGYATTHTLLRVSQTLLEKPGGYLSNDVMPPSLFLDNIPN
                     WEFGVLVQVRDLSRVLRNDFSRSQTQSVEDRDLAEVEPNFNFDNNSWILPSSESRYRQ
                     AIRRLERYQARLVGEAEPDANFYARADNLREWLAVVEKRLGNLSQQLSASVGTVRVNT
                     DLAGDPVADAAKPGLDRVEVKTPWLQIDDVFYEARGTAWALLHFLRAIEYDFRHVLAD
                     KNATVSLRQVIRELEASLAPMRSPMVLNGGGFGMFANHSLVMASYLSRANQAITNLRE
                     LLAGG"
     gene            7132..7395
                     /locus_tag="PLS34_04325"
     CDS             7132..7395
                     /locus_tag="PLS34_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86730.1"
                     /translation="MDKVHEQTDKELIALLPPEERADGRGHATKALVRFADGTMAIEE
                     LSGDKVQSLRPASRFRRSNPIFDPTTREVIGYEMEQVFLSATA"
     gene            complement(7496..8506)
                     /locus_tag="PLS34_04330"
     CDS             complement(7496..8506)
                     /locus_tag="PLS34_04330"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRP86731.1"
                     /translation="MGLLVKGEWRDEWYDTESTGGEFVRQDSAFRNWVTADGSPGPSG
                     EGGFRAESGRYHLYVSLACPWAHRTLIFRALKGLEDVISVSVVHPHMLENGWEFRDCE
                     AGAFTPGDPLFHSDYLYQVYLQARPDYTGRVTVPVLWDKERHTIVSNESADIIRMLNS
                     AFAAFASSDADYRPAELHDEIDAINARVYKQVNNGVYRCGFATTQKAYEEAFRALFET
                     LDWLDERLATRRYLLGDRITEADWRLFTTLVRFDAVYVGHFKTNLRRIQDYPQLSGYA
                     RELYQVPKVAQTVHFGHIKQHYYFSHRMINPTGVVPVGPALDLESETGVRPRFSHVKN
                     GV"
     gene            8574..8924
                     /locus_tag="PLS34_04335"
     CDS             8574..8924
                     /locus_tag="PLS34_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRP86732.1"
                     /translation="MTDESYAAMRKRISTANAGLKAAQPDTLKGFNLMHQQAMQDGAL
                     DPKTKELVAVALSVGSRCDPCIAFHVPAALRHGATREEIMEVLSVCVMMGGGPSLMYA
                     AHVIEALDEALGSS"
     gene            complement(8985..9479)
                     /locus_tag="PLS34_04340"
     CDS             complement(8985..9479)
                     /locus_tag="PLS34_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86733.1"
                     /translation="MQALNLNLNWTRELTADDGLDALSPLAPVAVAVAVEPARARAAR
                     AGLGPATGAMAGVLVAVLAMLAAPMTQPLIFALGGAAGFVYLGTALVSGRLDAALLDL
                     AAAAAAIGIAVTAAGPAVSVLLVHVVWGVLRGALVGAAPGRRFTTCWAAMHAVAALLL
                     GFGA"
     gene            complement(9559..10149)
                     /locus_tag="PLS34_04345"
     CDS             complement(9559..10149)
                     /locus_tag="PLS34_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP86734.1"
                     /translation="MTAPTDTREKILASADRLLRSYGFNGFSYQDIARPLGIRNAAVH
                     YHFSTKADLAVAVIENYRQLLHKRTAAFMAGHGDPVEQLEGFIAFSTAECCTESTMCP
                     VGALASDFYSLPEDIRVAGQRLLDELLAWMTRVCAVGREQGRMRFEGEPTAKAEQIVA
                     ALQGARQLARFRGAPAMQAVATQIRRDLGLGPRRDE"
     gene            complement(10245..11453)
                     /locus_tag="PLS34_04350"
     CDS             complement(10245..11453)
                     /locus_tag="PLS34_04350"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HRP86735.1"
                     /translation="MDDGLLILEADAGRRQLLAEYCAARGYRPRTVASYVEFAEAWQP
                     GLRMVALSLPLASSDGIDAIRFLGQQRCEAGLILTSDTDPRILSAASRLARARGLRVL
                     GTLMQPFSRADLAALLLHPEPELAAQTMVLAVQMAQEDLRDCLRRGLVRTWFQPKIAV
                     RSLEFVAVEALVRLEHPTRGLLRPSAFLALAEETGMIAELTELVAREAFTWCARWHRE
                     GLPIQVAVNISPLLLAELQLPDTLSGWAEELGVPPARIILEVTESWMMEDSVAALDTL
                     TRLRLGGFQLSIDDFGTGYSTMAQLHEIPYSEMKLDQRFVRTAALDHEARAIVESSIQ
                     LGHKLGMRVVAEGVERQEDWDLVSELGCDEGQGYFLARPMQADDLRAWLTRWNATLGL
                     ENAGAAGQAG"
BASE COUNT         1821 a         3976 c         4130 g         1758 t
ORIGIN      
        1 ggccgagcga gtttttcctt gtccgagcgc aggccggcct atggcgccgc gcgcctgcgg
       61 tcgaccggaa ccataggcgc cgcgcccgcg gtcagccagg ggaaaccggt gtctgacccc
      121 gtttaagcgg tggtttcggc gaactgcagc cgcgccaggc gggcatacag gccctcctgc
      181 gccacgagct cctcgtgcgt gccgacggcg accacacggc cggcctccag cacgacgatg
      241 cggtcggcct tcagcacggt ggcgaggcgg tgcgcgatca cgatggtcgt gcgctcggtc
      301 atcaggcgat cgagcgcctg ttgcaccagc cgctcgcttt cggcatccag tgcgctggtg
      361 gcctcgtcga gcagcagcag cggcgggttc ttgaggatgg cgcgggcgat ggcgatgcgc
      421 tgccgctggc cgcccgaaag ccgcacgccg cgttcgccga gataggtgtc gtagccctcg
      481 ggcatgtcgc agatgaactc gtgcgccagc gccgcgcggg cggcggcctc gacttcggcg
      541 tcgccggcct cgggacggcc gtagcgaatg ttctcgcgtg cggaggtgga gaagatcacc
      601 gtctcctgcg gcaccatcgc catggcgccg cgcaggtccg cgagcgcgag ctgcgcggtg
      661 tccatgccgt cgaacaggat gcgtcccgac tgcggatcgt agaagcgcag cagcaactgg
      721 aacaccgtgg tcttgccggc gccggacggg cccaccagcg cgacggtctt gccggcgggc
      781 accgtgaagg agacgtcgtc gagcgcccgg cgctccgggc gggcagggta gctgaagctg
      841 agctgctcca gccgcacctc gccgcgcgcg gggcgcgccg ccggcagcgg cgcggcgggg
      901 ctctggatgt ccggcgtggt ggtcaacagc tccatgatgc gttccatggc gcccgcggca
      961 cgctgcactt cgccccagac ctcggccagc gcggcaaaag agccggccac cagcaccgcg
     1021 tacagcacga actggccgag ctggccggtg gtcatcgtgc cgccgagcac ggcctgggcg
     1081 ccgaaccaca gcaccagcac gatggaaccg aacacgaaca ggatcaccac cgccgtcagc
     1141 agcgcccgcg cccggatccg cgccagcgcc gcgctgaacg aatcctccac ggcgtccttg
     1201 aagcgtgcgg cttcccatgc ctcgcgcacg taggcctgca cgatgcgcat cgcggtgaga
     1261 atttcctcgg cgagcgcact ggaatccgcg atccggtcct ggttgcgccg cgacagcagg
     1321 cgtacccgcc ggccgaacag gatcagcggc agcagcatga tgggcacgag cacgatgatc
     1381 agcccggtga gcttcgggct ggtgatgccc agcatcacga gaccgcccgc gagcagcagc
     1441 atgttgcgca acgcgatcga agcgctcgag ccgaccaccg actgcaggat ggtggtgtcg
     1501 gtggtcagcc gcgagagcac ttcgccggtg cgggtggtct cgtagaagga ctggctgagc
     1561 cgcaggatgt gccggaacac ggcctcgcgc acgtcggcca cgacgcgttc gcccagccag
     1621 ctgaccaggt agtagcgcag cgaggtggaa atcgccagca ggctggcaat gccgaacatg
     1681 gcgaggaaat agcggtccac gttggcggcg ttcgcggccg agaagccctc gtcgatcatg
     1741 aagcgcaccg ccaccggcag gctgagcgtg gcggccgccg ccagcagcag cgcgcccagc
     1801 gccgccagca gcatcccccg gtaaggcttc aggaacgggg cgaggtcgcg cagcggccgc
     1861 aggtcgcggc tgcgcggtcg gtcgacaccg tcgctgacgg tctcgctgcc atcggggttg
     1921 cgggtggtag ccatgtcgtg ggccgcggat tatacgggat gccgccgccg caaaggcgct
     1981 gcagcagcgc tccggcgcgg tcgccgtcag ggggcgccgg ccgccggccc gagctgcagc
     2041 gcgtcgtgca gttcgtcgat cgccatggtc tcgagccagc gggcgccgcg cgccagcagc
     2101 ccgcgggcca gcgttgcatc cgccacctcg taaagcaccc actcccaggc tccgcgtggc
     2161 agcggtcccg gcgccagccg ctcgtggttg cagaacagcc agcgggcggg cagcgcgcgg
     2221 gcacgcgtct cgatggccgg gtcgaagccg cgcacgatcc agccgagctc cggcgcaccc
     2281 aggtcgcccg ccagcgccag gatctcgtag tcgctggcaa tcgggaacca gcggccctgc
     2341 gccggcgtca tggcgtgcag gcaggcgtcc gccaccgcat tgcgcccgaa gcgctcgatg
     2401 ctctgactct tcagctcgac gaaggcgcag accccctcgg tgccggccag ccactgcgcg
     2461 aaccgggcca gggtcggcgc gcgcgcctcc gggaagcggg ggccgaagcg cgccggctcg
     2521 ccgaccggga tgtccgccag cgccgccgcc ggcaggtcga acacggcttc ggggcgtccc
     2581 gcgacgcgca cgaggtcggc atcgtgcagc agcaccggca cgccgtcagc gctcagctgg
     2641 acgtcgatct cgacccagcg cgcgccggcc gcgaccgcgg cctgcaacgc cggcagggtg
     2701 ttctcgggcc acgtggcggc cagtccgcgg tgtgcgacca gggtcgggtt gtctgtcatc
     2761 atggcgcaag catagaccgc cggggcggcg gcggttcgtc gcctcgtcga tccactcggg
     2821 tgtcgcatcg atcatgagct tcgtatcaca cctgcgcggc atcggttctg gggcctgcta
     2881 cccggccgac gcagtgatga acctctgccc ggtggacggc cggcccgtcg agatggtgct
     2941 ggacctcgag cgcctgcgcg cggcgcggcc cggcacgagc tggttgctac cgggacgccg
     3001 ggacatgtgg cgcttcggcg ccctgctgcc gctcgatgcc ggtgacccgg aggaagcacg
     3061 ccacatcgtc accctgggcg aaggcgccac accgctgctg gacgcgagcg cctggccgct
     3121 ggcccggcag ggtggcttcg aattgctggt caaggaagag ggacgccacg tgccaggctg
     3181 cggcgcgaac ccgacgcaca gcttcaagga tcgcggcatg gccatggtag ccacgcaagc
     3241 gcgccggctc gggttgcggc ggttggcggt gccgacccag gggaacgccg gcgattcgct
     3301 ggccgagtac gcgcgcgccg cggggctcga ggtggcggtg gtgatgcccg gggacacgcc
     3361 gcggggcgtg ctggatcgcg tggcgagcct ggcagccgcg gacccgcgca tccggctcga
     3421 gctggtcgag ggcaccatcc gcgaggccgg cgcgcgcgtg aaatcgcact gggtgccggc
     3481 cggctggttc agcgtcgcca ccttccagga gccgggctgg cgcatcgagg gcaagaagac
     3541 cctcgggctg gaactggcgg aacctgtgca gccgggcggc gcctggcggc tgcccgacgt
     3601 catcgtttat cccaccgggg gcggcaccgg gatcatcggc atgtggaagg cgttcgccga
     3661 gctggaggcg ctgggggtga tcggccccgt gcggccgcgc atggtggcgg tgcagagcga
     3721 agccacggcg cctctcgtcg cggcccttgc ggcgggcgcg gacgacacct gcgccggcac
     3781 cgccggcgcc acgctcgcgg tcggcctgaa cgtgcccggc ggcgtcgggc atttccgcgt
     3841 gctcgagatc ctgcgcgcca gcggcggcgg cgctctggcg gtggccgacg cagacatgcg
     3901 cgacgctttc cgggcggccc gccacacgct gggcatggac ctcgcgccgg agggcgccgc
     3961 ctgcatggcg gcgctgccca ggctgctcga cgagcgcctg gtgcggaccg gcgaccgggt
     4021 ggtggtggtc aacacctgcg cgccggagaa atacctgccg gagctgcggg actggtacga
     4081 ggcctgagcc ctaccagggc aacggctggc ctttcgcgtg ccagaagccg ccggtggtgg
     4141 tgatgtccag ctcgtccatg cgcgcgatga ggccggccgc cgcgtcctcc ggctcgacgt
     4201 cgccgccccc gccgaccatc tcggtgcgca cgtagccggg gtgcagcagc gcgacggcga
     4261 tgccgcgctc cttcaggtcc atggcgagcg accggcccgc cgcattcacc gcggccttgc
     4321 tcatgcgata gccgtagtag ccgccggaag tgttgtcctc catggagccc atgcggctgg
     4381 tgacgatgcc gatcttcgag ccctggtgca ggttgcccag cagcgcctgg gaaatgatca
     4441 acggcccgac tgaattcacc cggaactgca ggtagacgtc gtccagcgcc tcgcccttga
     4501 gcccgccgag tgtgtcgcgc cgcaggaccc cggcgttgtt gatcagccag tcgagcgact
     4561 cgccctcgac ggcccgggcg aggcgctgcg ccgaggccgg atcgcccacg tcgatgccgt
     4621 ccacgatgcg cacgccgagc gccttgagct cgctggagct ggtgcggcat gcggcgatga
     4681 ccgtgtcgcc gcgctcggcg agcgatgtgc acagggcaag cccgatgccg cgattggcgc
     4741 ccgtgaccag gcagttggcc atgatggtct cctttggatg ctgcgtgttt gtggccaagt
     4801 ataggcaggc cgcgctgtgg ccgataaagt agcagcatgg cgaatttgcg tatcgcatgg
     4861 ctgctggtgc tgctgttggc gacgggctgc gcgacgccgc gggcgccgac ggggctcgcc
     4921 atgccggcgc cggacgaggc gggggcggcc acgctgctgg caggctacct gctggcccag
     4981 gggtggaccg tgcgtctcgc cggcgaggcg gcggtggcgg cgcagcgcgg cgccgaacag
     5041 ctgttgctcg agccgctgct ggatccgcag gggctggaca ggattttggt gtcccggacc
     5101 tggccgcgcg ccgcgcaggc ggacgacgac gcgctcggcg catttgcgcg cgagctcaac
     5161 gagatgctca acgtgggcca gttccgcgtc tcgcccgacg gcctggtgct gcagtcgtcg
     5221 ctgccgttcc tcgacgcggt ggagccgcgc ctgttgaatg ccttcctcgc cttcacggcc
     5281 gacgtgcatt acgcgctgct gcaggtccag ggcgagcgcc agttgttggc ggcggttgag
     5341 ggcgacgcgg ccagccgcta atcttgccgc ttcgccccag ctttccggag cacgccccat
     5401 gagcgtcacc gtccgcgcgc gccacgagta tcgccatccc gtcagcaagg tgtgggcggc
     5461 cttcagcgac cccgagttct accaggccaa gttcgagggc atcgggcatc gcaacgtgga
     5521 ggtcgtcgcc acggaaagcg atgacgaagg cttctccatc gaagtcagcc gcgaagtacc
     5581 tctcgacgtg ccgggcttcc tgcgcagcat gctgggcgag tggaacaccc tgctgcagaa
     5641 cgagcgctgg agcgaggccg gcaagggcgt ctataccaac gagctcgaga tcgaggcgcg
     5701 cggcgtgccg gcggccatga ccggcaccat gaagctcacc agcaagggca agggctgcgt
     5761 caacgaggtg gccatcacca tccgcgctgc ggtgccgctg atcggcggca agctcgagca
     5821 gttcgtcgcc aaggataccg aggccaccct cgaggcagag tacgagttca tccaggatta
     5881 cctggacaac gcctgagacg ccgtaaaacc ggggtcagaa accggttttc ccgctagccg
     5941 ccggcgagca gttcgcgcag gttggtgatc gcctggttgg cgcgcgacag gtacgacgcc
     6001 atcaccagcg agtggttggc gaacatgccg aagccgccgc cgttgagcac catggggctg
     6061 cgcatcggtg ccaggctggc ttccagctct cgtatcacct ggcgcaggct cacggtcgcg
     6121 ttcttgtccg ccagcacgtg acggaagtcg tactcgatcg cgcgcaggaa gtgcagcagc
     6181 gcccacgccg tgccgcgcgc ctcgtagaac acgtcgtcga tctgcagcca gggcgtcttg
     6241 acctcgacgc gatccagtcc gggcttcgcc gcatcggcca cgggatcgcc cgcgaggtcg
     6301 gtgttgaccc gcaccgtgcc gacgctggcg gacagctgct gcgacaggtt gcccagtcgt
     6361 ttctccacca ccgccagcca ctcgcgcagg ttgtcggcgc gcgcgtagaa attggcgtcc
     6421 ggctcggcct cgcccaccag gcgcgcctgg tagcgttcca gccgccggat cgcctggcga
     6481 tagcggctct cgctcgaggg caggatccag ctgttgttgt cgaaattgaa gttgggctcg
     6541 acctcggcga ggtcccggtc ctccaccgac tgggtctggg agcggctgaa gtcgttgcgc
     6601 agcacgcgcg agaggtcgcg tacctgcacc agcacgccaa actcccagtt cgggatgttg
     6661 tcgaggaaca ggctgggcgg catcacgtcg ttgctcaggt agccgccggg cttttccagc
     6721 agcgtctgcg acacgcgcag cagcgtgtgg gtggtggcat acccggtcac tgcaggccgg
     6781 ccgtccttct cccagtccac ccagaacact gccggttcgc gcgaccagta ccagctcagt
     6841 ccgagcagca gcagcagcag cacggcgccg gccgcgccgc cggtcctggc gagcgcgcgc
     6901 cggttgcgcc agcccgggac tctgttgcgg aagccggaat ccttgaggtt catggcaaat
     6961 ctccgttgca atggcgccag tttaccgtcg cggcgtcact aagtgacgtg tttcgtcacc
     7021 cgggaggggc caggcggtgg tcttcaagta actgttttct atagaaacca tgttcttggc
     7081 atggtttctg cagggaatac ctcagtcaaa ttacccgcat gggaacgcat catggacaag
     7141 gtccacgagc aaacggacaa ggaactcatc gcgctgctgc cgcccgagga gcgtgctgac
     7201 ggccgcgggc acgcgaccaa ggcgctggtc cgcttcgctg atggcacaat ggccatcgag
     7261 gagctcagcg gcgacaaggt gcagtccctg cggccagcca gccgcttccg ccgctcgaat
     7321 ccgatcttcg atcccacgac gcgggaggtc atcggctacg aaatggagca ggttttcctt
     7381 tcggcgaccg cctgagcgct gccgccgcac gcattcgact ccccccttgg gcgccgcgat
     7441 cctcggatca tcatgcggcg ccttcttttt ttcgcgactg agtcagaaac tggggtcaga
     7501 ccccgttttt cacatgcgaa aaacggggtc tgaccccagt ttctgactcg aggtcgagcg
     7561 ccgggccgac cggcaccacg cccgtcgggt tgatcatccg gtggctgaag tagtagtgct
     7621 gcttgatgtg gccgaagtgc accgtctgcg ccaccttcgg cacctggtag agctcgcgcg
     7681 catagcccga cagctgcggg tagtcctgga tgcgtcgcag gttggtcttg aagtgaccga
     7741 catagacggc gtcgaaacgc accagcgtgg tgaacagccg ccagtcggcc tcagtgatcc
     7801 ggtcgcccaa gaggtaacgc cgcgtggcca ggcgctcgtc aagccagtcg agcgtctcga
     7861 acagcgccct gaaggcctcc tcgtaggcct tttgcgtggt ggcgaagccg caacggtaga
     7921 cgccgttgtt gacctgcttg tagacgcgcg cattgatcgc gtcgatctcg tcgtgcaact
     7981 cggccggccg gtagtcggcg tccgatgacg cgaaagccgc gaaggcgctg ttgagcatgc
     8041 ggatgatgtc cgccgactcg ttgctgacga tggtgtggcg ttccttgtcc cacagcaccg
     8101 ggacagtgac gcggccggtg tagtccggcc tggcctgcag gtagacctgg tacaggtagt
     8161 cgctgtggaa cagcgggtcg cccggcgtga aggcgccggc ctcacagtcg cggaactccc
     8221 agccgttctc gagcatgtgc ggatgcacga ccgagacgct gatgacgtct tccaggccct
     8281 tcagggcgcg gaagatcagc gtgcggtgcg cccacgggca ggccagcgag acgtagaggt
     8341 ggtagcggcc ggactcggcc cggaagccgc cctctccgct ggggccgggc gagccgtccg
     8401 ccgtcaccca gttgcggaac gccgagtcct ggcggacgaa ctcgccgccc gtcgactcgg
     8461 tgtcgtacca ctcgtcacgc cattcgccct ttaccagcag acccatgtca aaaaacgctc
     8521 ccggcgcagc ggttaagctg cctgcctgtt gtcttccaat tgaagggacc attatgaccg
     8581 atgaaagtta cgcggccatg cgcaagcgca tcagcacggc gaacgccggc ctcaaggccg
     8641 cgcagccgga cacgctgaaa ggcttcaacc tgatgcacca gcaggcgatg caggacggcg
     8701 cgctggaccc gaagaccaag gaactcgtcg cggtggcgct gtcggtgggg tcgcgctgcg
     8761 atccctgcat cgcctttcac gtgcccgcgg cactgcgcca cggtgcgacc cgcgaggaga
     8821 tcatggaggt gctgtcggta tgcgtgatga tgggcggcgg gccgtcgctg atgtacgcgg
     8881 cgcacgtcat cgaagcgctc gacgaggcgc tgggctcgtc ctgatcgcca ttgcggcgca
     8941 gggcaagggc cggggcgccg tctgccggac ggcgccccgg agtctcatgc gccgaagccg
     9001 agcagcagtg cggccacggc gtgcatcgcc gcccagcagg tcgtgaaacg ccggccgggg
     9061 gcggcgccga ccagggcgcc ccgcagcacg ccccacacca cgtgtaccag cagcaccgac
     9121 acggccggcc ccgcggcggt caccgcgatg ccgatcgcgg ccgcggccgc cgccaggtcc
     9181 agcaaggcgg cgtcgagccg gccggacacc agcgccgtgc cgagatagac gaaccccgca
     9241 gcgccgccga gcgcgaagat gagcggctgg gtcatcggcg cggcgagcat ggccagcacc
     9301 gccaccagga cgccggccat ggcgccggtc gcaggtccga gcccggcccg cgcggcccgg
     9361 gcccgggcag gctcgacggc gaccgcgacg gcgacggggg cgagcggcga aagcgcgtcc
     9421 aggccgtcgt ccgcggtgag ttcccgggtc cagttgaggt tcaagttgag cgcttgcatg
     9481 gtcactctcc tgcgttgttg cgcctggtgg atcaacctta cttatcgata agtaagtaag
     9541 gtttacaaaa aaattcggtc actcgtcgcg gcgcgggccg aggccgagat cccggcggat
     9601 ctgtgtggcg accgcctgca tggccggcgc cccgcgaaag cgcgccagct ggcgcgcgcc
     9661 ctgcagcgcc gccacgatct gctcggcctt cgcggtcggc tcgccctcga agcgcatccg
     9721 gccctgctcg cgaccgaccg cgcagacccg cgtcatccag gccagcagct cgtccagcag
     9781 tcgctggccg gcaacgcgga tgtcctccgg cagggaatag aaatccgagg ccagcgcgcc
     9841 gaccgggcac atggtgcttt ccgtgcagca ctcggcggtg gagaaggcga taaagccctc
     9901 gagctgttcc accgggtcgc cgtggcccgc catgaaggct gccgtgcgct tgtgcagcag
     9961 ctgccgatag ttctcgatca ccgccacggc gaggtccgcc ttggtggaaa aatggtaatg
    10021 gaccgcggcg ttacggatgc ccagcgggcg ggcgatgtcc tggtagctga agccgttgaa
    10081 gccatagctg cgcagcagcc ggtcggccga ggccaggatc ttttcacggg tgtcggtggg
    10141 cgcggtcatg tcgctcgttc cagcaaggac tgactaatta gtaagtaagt tgcggccaga
    10201 tgtcaatacc ccgggaaggt cggggcggcg ggcatgtcga ccgatcagcc ggcctggccg
    10261 gcggcgcccg cgttttccag cccgagggtg gcgttccagc gcgtcagcca ggcgcgcagg
    10321 tcgtcggcct gcatgggccg cgccaggaaa tagccctggc cctcgtcgca gccgagctcg
    10381 gacaccaggt cccagtcctc ctggcgctcc acaccctcgg ccaccacccg catgccgagc
    10441 ttgtgaccca gctggatgct ggactcgacg atcgcgcgcg cttcgtggtc cagcgccgcg
    10501 gtgcgcacga agcgctggtc gagcttcatc tcgctgtagg gaatctcgtg cagctgcgcc
    10561 atggtggaat agccggtgcc gaagtcgtcg atcgacagct ggaagccgcc gagccgcagg
    10621 cgggtgagcg tatcgagtgc cgcgacgctg tcctccatca tccagctctc ggtcacctcg
    10681 agaatgatcc gcgccggcgg aacgccgagc tcttcggccc agccggacag cgtatcgggc
    10741 agctggagtt ccgccagcag cagcggggaa atgttgactg ccacctggat gggcaggcct
    10801 tcgcggtgcc agcgggcgca ccaggtgaaa gcctcgcgtg cgaccagttc ggtcagctcc
    10861 gcaatcatgc cggtttcctc ggccagcgcg aggaaagcgc tcgggcgcag cagcccgcgg
    10921 gtggggtgct cgagccgcac cagcgcctcg accgcgacga actccaggct gcgcaccgca
    10981 atcttgggct ggaaccaggt cctgaccagc ccgcgccgca ggcagtcccg gagatcctcc
    11041 tgggccatct gcaccgccag caccatggtc tgtgccgcca gttccggctc cggatgcagg
    11101 agcagggcgg ccaggtcggc ccggctgaag ggctgcatga gcgtgccgag cacgcgcaga
    11161 ccgcgcgcgc gggcgagccg gctggccgca ctgaggatgc gcgggtcggt gtcgctggtc
    11221 aggatgagtc cggcctcgca gcgctgctgg ccgaggaaac gaatcgcgtc gatgccgtcc
    11281 gagctggcca gcggcagcga cagcgccacc atcctcaggc ccggctgcca ggcttcggcg
    11341 aactcgacgt agctggccac cgtgcgcggg cgatagccgc gcgccgcgca gtactccgcg
    11401 agcaactgcc ggcggccggc gtcggcctcg aggatcagca ggccgtcgtc catcgtcgtt
    11461 ccaaagccgg cggcccgcag ccgggggccg cggatacctg gcagacagaa tcgtccatgc
    11521 gtgtaccccc gttccgtgtg atacacgcgc cgagcatagc aacaaagtcg tgcgcggctg
    11581 catagcgggt cacagtttgc cgaccgatgt tttcgctgcg atgcgacagg ccgggcgcgt
    11641 ataatcgcca gtccgccaca gcggcgagga ccggtccggg gggcc
//
LOCUS       DAOMPG010000052        11642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2383_length_11642_cov_4.021317, whole genome shotgun sequence.
ACCESSION   DAOMPG010000052 DAOMPG010000000
VERSION     DAOMPG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2383_length_11642_cov_4.021317"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLS34_04355"
     CDS             <1..859
                     /locus_tag="PLS34_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRP86736.1"
                     /translation="VIFRENTEDIYAGIEFEQGSAAAKRFLDLFREHFPDEFRKIRFP
                     DTSGIGIKPVSREGTERLVRAAIQYAIDNGRKSVTFVHKGNIMKFTEGAFRKWGYALA
                     EREFGDRTYTWDQWEHTRDEQGEAAANAEQKKALAGGKVLIKDAIADITLQQVLTRPS
                     EFDVIATLNLNGDYLSDALAAQVGGIGIAPGGNINSVTGHEVFEATHGTAPKYANLDK
                     VNPGSLVLSGEMMLRHLGWLEAADAIITAMDKTIGSKTVTYDFARLMDGAREVKCSEF
                     ADALIGNLG"
     gene            884..2611
                     /gene="aceK"
                     /locus_tag="PLS34_04360"
     CDS             884..2611
                     /gene="aceK"
                     /locus_tag="PLS34_04360"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRP86737.1"
                     /translation="MPDSALARDCARAIFDAFGAYNAEFREVTRRARLRFETRDWVAG
                     RRDATERIDLYDRYVAETTEMLRGRLGPRATDRDLWVRIRTCFEDEIRDFIDLEFEKT
                     FFSSISRRLFATIGVDPEVEFVALDLDPLSKIRPPQALNIYEFSGDPATMVEEVLEDH
                     SFESPWEDFKASVDFVAAELARAWEPLGGIERLARIEFMRPIYYQNTRAYVVGRALGA
                     AQPQPVVIALKNSVDGVSVDAVLTSVRDTSILFGFTRSYFHADLETVHDAVVFLRTIL
                     PRKPLAELFTVLGRARQGKTETYRTFFRHLQNSRDLLIEAPGDRGMVMAVFTLPSFDV
                     VFKVIRDRFAYPKTVVRKDVLDKYYLVFRHDRAGRLVDAQEFKMLRFPRTRFDPALLE
                     HLLETAAQTVFVDGDDLVITHCYVERRLRPLNLYLAEASQLAARHAVLDYGQAIRDLA
                     ATNIFPGDLLLKNFGVTRNGRVIFYDYDELCLVTECNFRKLPRPRTIEEEMQSEAWFF
                     VDEDDVFPEQFISFLGLEPQQLEVFLENHAELLTPEFWREMKRRHEAGEVLEILPYRP
                     TLSGGGGSA"
     gene            2604..3329
                     /locus_tag="PLS34_04365"
     CDS             2604..3329
                     /locus_tag="PLS34_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HRP86738.1"
                     /translation="MPDGRDRGGRRHLWLLLPAAALAALWIAWRALALADFLYPVFYD
                     ALDVHGHIQTFAPQNRYKADFGTTTREERLRLFGAIVDGIHASGEGLAELEYHRRDGQ
                     VIDTLLRAPEVGHLQDVAKLVDRIALVGWLAVAWTAAHLLLIRLFGWRVPSLTRLLGT
                     SLLAALAGVATVLLIGARRVFYWFHEWVFPPDHPWFFYYQDSLMSTMMKAPDLFGAIA
                     AALLALGLAVYALLLWLARRAQG"
     gene            complement(3326..3622)
                     /locus_tag="PLS34_04370"
     CDS             complement(3326..3622)
                     /locus_tag="PLS34_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86739.1"
                     /translation="MEWGMLIGSIVLIVAAFGPLRAYIAASDSPGVQRFRSWHKPMEW
                     FGAAALMISALGILFLPEGGVPAVIPTLGAIVFYASMLTRPGPANQGGAGGPGH"
     gene            complement(3734..4546)
                     /locus_tag="PLS34_04375"
     CDS             complement(3734..4546)
                     /locus_tag="PLS34_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86740.1"
                     /translation="MIREIVMREKSQFIALATAVVGGLLLMPAPCEARASVGGQDKAG
                     FERVVPDIAHARDYGIEHYMVQSQPYRTTITPVGQGGKRLGKIVVTKRPDEFGLRIED
                     TDSAMRDWSFRKLADDRFLVRLQSRSATGRAETSVFHYDAAAAEMLAVDPDGEITMVP
                     EPLACSDADPRGARAPAQAEDLTAVGAALTDPNLRGYLPEELRGFTAGRDGAVFLASS
                     CTSCIASCGACVACAMAGCGGAVACAGCAGACLMCCCSDACKDCYRDFRDEK"
     gene            complement(4702..6087)
                     /locus_tag="PLS34_04380"
     CDS             complement(4702..6087)
                     /locus_tag="PLS34_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRP86741.1"
                     /translation="MPASDFYTDRTAAACPIHPVDTAGLAALLGKLADAHRGWVRSTG
                     WQAKPGTALALPGSDGSVAAVLAGTGGAEPFWSLAALPGQLPAGSYVFADEAGSGEST
                     RRALAWGIGQYAFGRQSNFKRAEPRKLAWPEGVDTAAALSTVDADALVRDLVNTPAGD
                     MGPAELSQAAAALAASSGAEFREIVGDALLAENFPAIHAVGRASSRAPRLLELRWGEL
                     GRPRVTLVGKGVCFDTGGLNLKQPQGMRNMKKDMGGAAHVLGLAKMVMDAGLPVSLRV
                     LIPAVENSVAGNAYRPGDVVPTRAGLTVEIGNTDAEGRVVLADALALADEEAPELLLD
                     FATLTGAARVALGPDLPALFATDEELARDWAAAGADQGDPLWPMPLWAPYAANLGGGI
                     ADLNNVTTDGFAGAIYGGLFLQRFVTKAKSWAHVDTFAWNPRSRGGRPEGAAAQGIRA
                     AFRVLAQHYPA"
     gene            6275..6523
                     /locus_tag="PLS34_04385"
     CDS             6275..6523
                     /locus_tag="PLS34_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HRP86742.1"
                     /translation="MIELFRLFITPIRVEDSKFLSALQIKSCHDLKITLMQVAVVIMV
                     ILFLEHAVEIGATLETLYFGAGVALVMSAAVFSWKNMK"
     gene            6520..7023
                     /locus_tag="PLS34_04390"
     CDS             6520..7023
                     /locus_tag="PLS34_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3)-I family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HRP86743.1"
                     /translation="MRSRPDSPASIRRLTAADLPLMDALCRLFGEAFEDVETYTAHRP
                     SAGYLRGLLGGESFIALAALKNERVVGGLAAYELRKFEQERSEIYIYDLAVAAGHRRA
                     GIATALISDLASLAAERGAWVMFVQADTGPDDQAAIALYTKLGKREEVLHFDIPVDRR
                     HTLTTRR"
     gene            7041..7607
                     /locus_tag="PLS34_04395"
     CDS             7041..7607
                     /locus_tag="PLS34_04395"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRP86744.1"
                     /translation="MTASASPDFPELRTPRFLLRRIIASDLPAVYAGLSDVRVVTNYG
                     VSYDSLEETQQELDWYEELFRSGSGVWWGICAPERRAALLGTCGLNQIVREHRRAELG
                     YWLFPDYWGTGAARECVAAVLTHAFDSMGLHRVGAEVDIDNPRSSRLLERLGFQFEGI
                     RRGYELKDGVFLDLKYYARLSTDPEPAA"
     gene            7630..8280
                     /locus_tag="PLS34_04400"
     CDS             7630..8280
                     /locus_tag="PLS34_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vat family streptogramin A O-acetyltransferase"
                     /protein_id="HRP86745.1"
                     /translation="MHGPDPATKHPMDGFPQVCFIKNTVTNPNIIVGDYSYYDDPEDS
                     EGFERNVLYHFPFIGDRLVIGKFCAIGRGARFIMNGANHKLGGISTYPFEIFGKGWEK
                     AAPQPAEMPLKGDTIVGNDVWIGYEALVMPGVRIGNGAVVASRAVVASDVPPYAIVGG
                     NPARLIKQRFDAEAVAVLESLAWWDWPAEVITRHLDLIVSGDVAALREVAPHTPSE"
     gene            8287..8700
                     /gene="rnk"
                     /locus_tag="PLS34_04405"
     CDS             8287..8700
                     /gene="rnk"
                     /locus_tag="PLS34_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRP86746.1"
                     /translation="MNARPNIVISSLDAERLEALLASLPKDSFPGRAELEAELARAEI
                     VDPKDIPSTVVTMNSTVRFRVASSTEEFILTLVYPRDMDSSSSKISILAPVGSALLGL
                     AQGDEIEWPKPGGGRLRVRIEEVLYQPERAGVFHR"
     gene            8835..9563
                     /locus_tag="PLS34_04410"
     CDS             8835..9563
                     /locus_tag="PLS34_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP86747.1"
                     /translation="MTSVDEIAPEVFRISTFVPEVNLHFNQFLVRDEEPLLYHTGMRW
                     LFPAVRDAVAQLIDPAALRWIGFSHFESDECGSLREWQELAPQATAVCGHIGKNVSVD
                     DVVALRPARGLDDDEVFSTGQYRFRFLATPHVPHCWDAGLLFEETKGTLFCSDLLHQN
                     GQVEPLTASDVVGRFRQTLESYEKGPFAGYMPYTHNTPVTLERLAKLEPKTLAVMHGS
                     AYTGDGARAIRDMGEVLRDVLGPQ"
     gene            complement(9613..10092)
                     /locus_tag="PLS34_04415"
     CDS             complement(9613..10092)
                     /locus_tag="PLS34_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRP86748.1"
                     /translation="MNPRRTLVASALLASAVIAHAMSDMPYHGDGHIMVNASDLEWGP
                     IASMAPGAKFALLEGDLSKEAPFTFRLALPADYVIAPHIHPAYERVTVLSGTFHFGHG
                     REFDRERTVALEQGGLAIMAPGEPMFGFTEEPTVIQLHGMGPWGIEYLDPADDPRQQ"
     gene            complement(10212..10871)
                     /locus_tag="PLS34_04420"
     CDS             complement(10212..10871)
                     /locus_tag="PLS34_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRP86749.1"
                     /translation="MQILAEVLSPGLRLACAVLAGLLFLAALPGLRALRGHPERLHLL
                     LGSAVALALLWSIHARVDPGLALHVLGTPAVVLLLGWRLGTLAAALASLGLLATGATD
                     PGGAPAGWLLSAALPGVVIATVARLARFHLPRNPFIFIFVCAFFGSGLAVIATWLGGA
                     AMLAASGQPSPSGPGSSLLAFLPLVLFPEAFINGAVMSMLIVYRPGWVRLYDERFYDS
                     A"
     gene            complement(10998..>11642)
                     /locus_tag="PLS34_04425"
     CDS             complement(10998..>11642)
                     /locus_tag="PLS34_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP86750.1"
                     /translation="IGIIAGGVLGAIVGGPPGAIAGMAVGGVTVDREQKIRRNGELSQ
                     SVAALTVERDSLQSDNRSQKARLADLAQRLGELQALAESRVDAGLLAQGLELEVGFRT
                     DSASLPDGASDALEALAGLLQAAPEMKVHLEGYADPRGTERHNLNLSAARAEAVRDRL
                     VEAGIAPERIRLTAHGAPAALAPEVSADPDGWALQRRVSIRLESGDGQFAAHSH"
BASE COUNT         1956 a         4005 c         3794 g         1887 t
ORIGIN      
        1 ggtcattttc cgtgaaaaca ccgaggacat ctacgccggc atcgagttcg agcagggctc
       61 ggcggcggcc aagcgcttcc tcgacctgtt ccgtgagcat ttcccggatg agttccgcaa
      121 gatccgcttc cctgacacca gcggcatagg catcaagccg gtatcccgcg agggcaccga
      181 acggctggta cgcgctgcga tccagtacgc gatcgacaac ggccgcaaga gcgtgacctt
      241 cgtccacaag ggcaacatca tgaagttcac cgagggcgcc ttccgcaagt ggggctacgc
      301 cctggcggag cgcgagttcg gcgaccgcac ctacacctgg gaccagtggg agcacacccg
      361 cgacgaacag ggcgaagcgg ccgccaacgc cgagcagaag aaggcgctcg cgggcggcaa
      421 ggtgctcatc aaggacgcca ttgccgacat tacgctgcag caggtgctga cgcggccctc
      481 cgagttcgac gtcatcgcca cgctcaacct gaacggcgac tacctgtcgg acgcgctggc
      541 cgcgcaggtc ggcggcatcg gcatcgcgcc gggcggcaac atcaactcgg ttaccggcca
      601 tgaggtattc gaggccaccc acggcacggc gccgaagtac gccaacctcg acaaggtcaa
      661 cccgggcagc ctggtcctgt ccggcgaaat gatgctgcgt catctcggct ggctcgaggc
      721 ggccgacgcc atcatcaccg cgatggacaa gaccatcggc agcaagaccg tgacctacga
      781 ttttgcgcgc ctgatggacg gcgccaggga agtgaagtgc agcgagttcg ccgacgcact
      841 gatcggcaac ctcggctgac gccctgccgg gatgcctgac cgggtgcctg attcggccct
      901 ggcgcgtgac tgcgccaggg ccattttcga cgccttcggc gcctacaacg cggagtttcg
      961 cgaggtcacg cggcgcgccc ggctgcgttt cgaaacgcgc gactgggtag ccggccggcg
     1021 cgacgccacg gaacgcatcg acctctacga ccgttacgtt gccgagacca ccgagatgct
     1081 gcgcggccgg ctcggcccgc gcgccaccga ccgcgacctg tgggtgcgca tcaggacctg
     1141 cttcgaggac gagatccggg acttcatcga cctcgagttc gagaagacct ttttcagttc
     1201 catctcgcgc cgcctgtttg ccaccatcgg cgtcgacccc gaggtcgagt tcgtcgccct
     1261 ggatctcgac ccgctctcca agatccgccc gccgcaggcg ctcaacatct acgagttcag
     1321 cggcgacccg gccaccatgg tcgaggaggt gctggaggac cacagcttcg aatcaccctg
     1381 ggaagacttc aaggcgagcg tcgatttcgt ggccgcggag ctggcgcgcg cctgggagcc
     1441 gctgggcggc atcgagcggc ttgcgcgcat cgagttcatg cggccgatct actaccagaa
     1501 cacgcgcgcc tacgtggtcg ggcgcgcgct cggcgcggcc caaccccaac ccgtggtgat
     1561 tgcgctgaag aactcggttg acggcgtgtc ggtggacgcg gtgctcacct cggtgcgcga
     1621 caccagcatc ctgttcggct tcacgcgcag ctatttccat gccgatctcg agaccgtgca
     1681 cgacgccgtg gtcttcctgc gcaccattct gccgcgcaag ccgctggccg agctgttcac
     1741 cgtgctcggc cgcgcgcgcc agggcaagac cgagacttac cggacctttt tccgtcacct
     1801 gcagaattca cgcgacctgt tgatcgaggc gccgggcgat cgtggcatgg tcatggcggt
     1861 gttcacgctg ccctccttcg acgtggtgtt caaggtcatc cgcgaccgct tcgcgtaccc
     1921 caagaccgtg gtgcgcaagg acgtgctgga caagtactac ctggtattcc ggcacgatcg
     1981 tgccggccga ttggtggacg cccaggaatt caagatgctg cgctttccgc gcacgcgttt
     2041 cgaccccgcc ctgctcgagc acctgctgga gacggcggcg cagaccgtgt tcgtcgatgg
     2101 cgacgacctg gtcatcactc attgctacgt cgagcgccgc ctgcggccgc tgaatctcta
     2161 cctggccgaa gcctcgcaac tggcggcgcg tcacgcggtg ctcgattacg gccaggcgat
     2221 tcgcgatctc gccgccacca acatctttcc cggcgacctg ctgctaaaga atttcggggt
     2281 gacgcgcaac ggccgcgtga tcttctacga ttacgacgag ctctgcctgg tcacggagtg
     2341 caatttccgc aagctgccgc ggccgcgcac catcgaggaa gagatgcagt ccgaggcatg
     2401 gttcttcgtc gatgaggacg atgtcttccc cgagcagttc atcagctttc tcgggctgga
     2461 gccgcaacag ctcgaggtgt tcctcgagaa ccacgcggaa ctcctgacgc ccgagttctg
     2521 gcgcgagatg aagcgccgcc acgaggccgg cgaagtgctg gagatcctgc cctatcggcc
     2581 cacgctgtcg ggcggcggcg gcagtgcctg acggccgcga ccggggcggc cgcaggcacc
     2641 tctggctgct gctgccggcc gccgcgctgg cggcgctgtg gatcgcctgg cgggcgctcg
     2701 cgctggcgga ctttctctat ccggtgttct acgacgccct cgacgtgcac ggccacatcc
     2761 agactttcgc tccgcaaaac cgctacaagg cggacttcgg cacgaccacg cgcgaggaac
     2821 gactgcggct gttcggtgca atcgtcgacg gcatccacgc ctccggtgag ggcctcgccg
     2881 agctcgaata tcaccgccgc gacggccagg tgatcgacac cctgttgcgc gcgccggagg
     2941 tcggccacct gcaggacgtc gcgaagctcg tggatcggat cgcgctcgtc ggctggctgg
     3001 ccgtcgcctg gaccgccgct cacctgctgc tgatccggct gtttggctgg cgcgtgccgt
     3061 cactcacgcg gctgctcggc acgagcctgc tggcggcgct ggccggcgtg gccaccgtgc
     3121 tcctgatcgg cgcccggcgg gtgttctact ggtttcacga atgggtgttc ccgccggatc
     3181 atccctggtt cttctattac caggactcgc tgatgtccac gatgatgaag gccccggacc
     3241 tgttcggcgc catcgcggca gcgctgctcg ccctgggact cgccgtctat gcgttgctgc
     3301 tctggctcgc ccggcgggcc cagggctaat gccccgggcc gcccgcccca ccctggtttg
     3361 ccggcccagg ccgcgtgagc atggaagcat agaacacgat tgccccaagg gtaggaatca
     3421 ccgcagggac tccgccctcc ggcaggaaca agatgcccag cgcgctgatc atcaaagcgg
     3481 cggcgccgaa ccactccatc ggcttgtgcc aactgcggaa tcgctgcact cccggtgaat
     3541 ccgaagcagc aatgtaggcg cgcagcggac caaatgccgc cacgatgagg acaatcgagc
     3601 cgatcaacat tccccattcc attcttcccc tccattccgg gattcgtccc ggtcgtttgc
     3661 cgccaggcca tgaaccattg cggggtcgtc ccccgggcag ggaatgatcc ccgcccgggg
     3721 cctgtattcc cggctacttc tcgtcccgga agtcccgata acaatccttg caggcgtcgc
     3781 tgcaacaaca catcaagcag gctccggcac accctgcgca ggcgaccgcg ccgccgcagc
     3841 cagccatcgc gcaggcaacg caggctccac aactggcgat gcacgacgtg cagctgctcg
     3901 ccaggaagac cgccccgtcc cgaccggccg tgaaaccacg cagctcctcg ggaagatacc
     3961 cgcggagatt gggatcggta agcgccgcac caacggcggt caggtcttcc gcttgcgcgg
     4021 gggcacgcgc gccacgcggg tccgcgtcgc tgcaggccag cggctcgggg accatcgtga
     4081 tctcaccgtc cgggtcgacg gcgagcatct cggcggccgc agcatcgtag tgaaacacgc
     4141 tggtctcagc ccggccggtg gctgaacggg actgcaggcg caccaggaag cggtcgtccg
     4201 ccagcttccg gaagctccag tcgcgcatgg cggagtcggt gtcctcgata cgcagtccga
     4261 attcatcggg acgcttggtg accacgatct tgccgagccg cttgccgccc tgcccaaccg
     4321 gcgtgatcgt ggtgcgatac ggctgtgact ggaccatgta gtgctcaatc ccgtaatcgc
     4381 gggcgtgtgc gatatcgggc accacccgct cgaaacccgc cttgtcctgg ccgccgacgg
     4441 acgctcttgc ctcgcacggc gccggcatca gcagcaggcc acccacgacg gccgtggcca
     4501 gagcgatgaa ctgactcttt tccctcatga cgatctccct gatcaacgtg gtactggtaa
     4561 aggaagcacc actgtctcag gaagatcgcc tgatccgtat atgttctttc tgcgccatat
     4621 acagattaat gcaggaagac cggatagtca cccatggagc gggcgtccgg cggccgggcg
     4681 gctgcggcgg gcggtctcct gctatgccgg atagtgctgc gccagcaccc tgaacgccgc
     4741 ccgtatcccc tgcgcggcgg cgccctccgg ccgtccgccg cggctgcgcg ggttccaggc
     4801 gaaagtatcc acatgcgccc aggatttcgc cttggtcacg aagcgctgca agaacaggcc
     4861 gccatagatt gcgccggcaa agccgtcggt ggtgacgttg ttcaggtccg caatgccacc
     4921 gcccaggttc gctgcatacg gcgcccacag cggcatcggc cacaatgggt cgccctggtc
     4981 cgcccccgcc gccgcccagt cgcgcgccag ctcctcgtcg gtggcgaaca gcgccggcag
     5041 gtccgggccg agcgctacgc gtgctgcgcc ggtgagcgtg gcgaaatcca gcagcagttc
     5101 cggcgcttcc tcgtcggcca gcgccagcgc atcggccagc accacgcggc cttccgcatc
     5161 ggtgttgccg atttccaccg tcaggcccgc gcgcgttggc accacgtcac ccggccggta
     5221 ggcattgccg gcgacgctgt tctccaccgc cgggatgagg acccgcaggc tcaccggcag
     5281 gccggcgtcc atgaccatct tggcaagccc gagcacgtgc gccgccccgc ccatgtcctt
     5341 cttcatgttg cgcatgccct gcggctgctt caggttcaac ccgccggtgt cgaagcacac
     5401 gcccttgccc accagcgtga cacgcggtcg gcctaactcg ccccagcgca gctcgagcag
     5461 ccgcggcgcg cgcgagctgg cgcggcccac tgcgtggatg gccggaaagt tttccgccag
     5521 cagggcgtcg ccgacgattt ccctgaattc ggcgcctgag gacgccgcca gggccgcggc
     5581 cgcctgcgac agctcggccg gtcccatgtc gccggccggc gtgttcacca ggtcgcgcac
     5641 cagcgcatcc gcgtccaccg tcgagagagc cgccgccgtg tccacgccct ccggccacgc
     5701 aagcttgcgc ggctcggccc gcttgaagtt cgactgcctg ccgaaagcgt actggccgat
     5761 gccccaggcc agcgcccgcc gcgtcgactc gccactaccc gcctcgtccg caaagacgta
     5821 gctgccggcc ggcaattgcc cgggcagagc cgccaacgac cagaacggct ccgcgccgcc
     5881 agtgccagcc agcaccgccg cgacgctccc gtcactgccc ggcagcgcca gggcggtgcc
     5941 cggcttcgcc tgccagccgg tgctgcgcac ccagccgcga tgggcgtcgg cgagcttgcc
     6001 gagcagcgcg gcgaggccgg ccgtgtccac cgggtggatc gggcaggcgg cggcggtgcg
     6061 gtcggtgtag aaatcgctgg cgggcatatc ggcatccttg gctcgactcg gcggacggca
     6121 tgattatgcc attcgcggcc gccccggccg cagcggattc attgacacgg ggcaggtgtg
     6181 gcatattgcc gggctggcgc ccggtttgcc ggacgccggc ggtgcgctgg gagttcctgc
     6241 cgcagcggag tcatgcacaa cacgggccgg cgaaatgatc gaacttttcc gcctgttcat
     6301 cacccccatc cgcgtggaag atagcaagtt tctcagcgcg ctccagatca agagttgcca
     6361 tgacctcaag atcacgttga tgcaggtggc cgtggtgatc atggtcatcc tgtttctcga
     6421 gcatgccgtc gaaatcggcg ccacactgga aacgctgtac ttcggcgcgg gggtcgcgct
     6481 ggtcatgtcg gccgccgtat tttcttggaa gaacatgaaa tgagatcgcg accggactcc
     6541 cccgcatcca ttcgccgact caccgccgcc gacctcccgc tgatggatgc gctgtgccga
     6601 ttgttcggcg aggcgttcga agatgtggag acatacaccg cgcaccggcc gagtgccggc
     6661 tatctccgcg gactgctggg cggcgagtcg ttcattgcgc tggccgccct gaagaacgag
     6721 cgggtggtgg gcgggctggc cgcctatgag ctcaggaagt tcgaacagga gcggagcgag
     6781 atttacatct acgacctggc cgtcgccgcg ggccatcggc gcgcgggcat cgcaacggcc
     6841 ctgatctccg acctggcctc gcttgccgcc gagcgtggcg cctgggtgat gttcgtccag
     6901 gcagacacgg gccccgacga ccaggcggcc atcgcgctgt acacgaagct ggggaagcgt
     6961 gaagaagtgc tgcacttcga cattccggtc gatcgccggc acacgctcac cacgcgtcga
     7021 tgagccggcg acaatcgatc atgaccgcat ccgcaagccc cgacttcccg gaactgcgca
     7081 ctccgcggtt cctcctgcga cgaattattg cgagcgacct gccagccgta tacgcgggct
     7141 tgtccgacgt gcgggtcgtc acgaactacg gggtcagcta cgactcgctc gaggaaacac
     7201 agcaggaact ggactggtac gaagagctgt tccggagcgg cagcggggtc tggtggggta
     7261 tctgtgcccc cgagcggcgc gcggccctgc tggggacctg tggcctgaac cagatcgtcc
     7321 gcgagcaccg gcgcgcggaa ctgggctact ggctctttcc ggactactgg ggcacgggag
     7381 cggcgcggga gtgcgtggcc gcggtgctca cccatgcgtt cgattccatg ggactgcacc
     7441 gggtcggcgc cgaggtggac atcgacaacc cgaggagctc caggttgctc gagcggctcg
     7501 gcttccagtt cgagggaatc cgccgcggct acgagctcaa ggacggcgtg ttcctcgacc
     7561 tgaaatacta cgcccgcctg tccaccgatc cggagccggc cgcctgaagc actttcggag
     7621 gaacccacca tgcacggccc cgatcccgcc accaagcacc cgatggacgg ctttccgcaa
     7681 gtctgcttca tcaagaatac ggtgacgaac ccgaatatca tcgtcggcga ctacagctat
     7741 tacgacgacc ccgaggattc ggagggattc gagcgcaacg tcctgtatca ctttcccttc
     7801 atcggcgacc ggctcgtcat cggcaagttc tgcgccatcg ggcgcggcgc gcggttcatc
     7861 atgaacggtg ccaaccacaa gctgggtggc atttcgactt atcccttcga aatcttcggc
     7921 aaagggtggg aaaaggccgc cccgcagccg gccgaaatgc ccctcaaagg tgataccata
     7981 gtcggcaacg acgtctggat cgggtacgag gcgctcgtta tgccgggtgt caggatcggc
     8041 aacggggccg tcgtggcgtc ccgggccgtg gtcgcatcgg acgtgccgcc gtatgccatc
     8101 gtcggcggca accccgcccg actcatcaaa cagcgcttcg acgccgaggc cgtggccgtc
     8161 ctcgagtcgc tcgcgtggtg ggattggccg gcagaagtca tcacccggca cctggacctg
     8221 atcgtttcgg gcgacgtcgc ggcattgcgc gaggtcgcgc ctcatacacc cagtgagtag
     8281 acagcaatga acgccagacc caatatcgtg atctcgtcgc tggatgccga aaggctcgag
     8341 gcgctgttgg cgagcctgcc gaaggactcg ttcccggggc gcgccgaact ggaggccgaa
     8401 ctggcccgtg ccgaaatcgt cgacccgaag gacattccct cgacagtcgt caccatgaat
     8461 tcgacggtgc gctttcgcgt cgcctcctcg acggaggagt tcatcctgac gctcgtgtac
     8521 ccacgggaca tggattccag cagcagcaag atttccatcc tggccccggt cggcagcgcg
     8581 ctgctgggcc tggcccaggg cgacgagatc gaatggccga agcccggcgg cggccgtctg
     8641 cgggtgcgca tcgaagaagt gctgtatcag cccgagcgcg ccggcgtctt ccatcgctga
     8701 gccccaggtc cgcgctcggg ctgtgctccc aggccgactg ggagcgggtt tggcgccggt
     8761 gccggtcggg gcagacaaaa ggcggatatt ggtctttact gcagcatcgt gttgccgctg
     8821 caggagcgcc cgccatgacc tcagtcgacg aaatcgcccc ggaagtattc cgcatctcga
     8881 ccttcgtgcc cgaggtgaac ctgcatttca accagttcct ggtgcgtgac gaagaaccgc
     8941 tgctctacca caccggcatg cggtggctgt ttccggccgt acgcgacgcc gtggcgcagc
     9001 tcatcgaccc ggcagccctc aggtggatcg gcttcagcca cttcgagtcc gacgagtgcg
     9061 ggtcgctgcg cgagtggcag gaactggcgc cgcaggcgac ggccgtctgc ggccacattg
     9121 gcaagaacgt cagcgtcgac gacgtggtcg cgctgcggcc cgcccgtggc ctggacgacg
     9181 acgaagtgtt cagcacgggc caataccgat tccgcttcct ggccacgccg cacgtgccgc
     9241 actgctggga cgccggcctg ctcttcgagg agacaaaggg cacgctgttc tgctccgacc
     9301 tgctgcacca gaacggccag gtcgagccgc tgactgcgtc ggacgttgtg ggccgcttcc
     9361 gccagaccct ggagagctac gagaagggac ccttcgccgg ctacatgccc tacacgcaca
     9421 acacgccggt gaccctcgag cggctcgcga agctcgagcc gaagacgctg gcggtgatgc
     9481 acggctcggc gtacacgggc gacggcgccc gcgccatccg cgacatgggc gaggtgctca
     9541 gggacgtgct cggaccacag taggccggat tatcggcagc cggccgaccg tgctcgagga
     9601 cggcgttgcc ggctactgct ggcgcggatc gtccgcggga tcgaggtact cgatgcccca
     9661 gggtcccatg ccatgcagct ggatgacggt cggttcctcc gtgaaaccga acatcggctc
     9721 accgggggcc atgatggcca gtccgccctg ctccagcgcc acggtgcgct cgcgatcgaa
     9781 ctcgcggccg tggccgaaat gaaaggtccc ggacagaacc gtgacccgct cataggccgg
     9841 atggatgtgc ggcgcaatga cgtagtcggc gggcaacgcc aggcggaaag tgaacggtgc
     9901 ctccttcgac aggtcgccct cgagcagcgc gaacttcgct cccggcgcca tcgaggcgat
     9961 cgggccccat tcgagatctg atgcgttgac catgatgtgt ccgtcaccgt ggtaaggcat
    10021 gtccgacatg gcatgcgcaa tgacggcgga cgcgagcagt gcggacgcga cgagcgttct
    10081 gcgtgggttc atgacaacct cctccggacg tttcgtcttt ctggattgcg ccctctacca
    10141 tagaaaatcg tcgccgcatg tcaaactggg ttgtatgcgc gatcgttgcg cgtccgcttc
    10201 gcggccgtgc atcaggcgct atcgtagaag cgctcgtcgt agagcctgac ccagccaggc
    10261 cgatagacga tcagcatgct catcaccgcg ccgttgatga atgcctcagg aaacagcacc
    10321 agcggcagga acgcgagcag cgagctccct ggtccgctcg gcgagggctg gccggacgcc
    10381 gcgagcatgg cggcgccgcc cagccaggtg gcgatgaccg ccaggcccga accaaagaac
    10441 gcgcacacga agataaaaat gaaaggattg cgcggcaggt gaaagcgcgc cagccgggcg
    10501 acggtggcga tcaccactcc cggcagggcc gctgacagca gccagcccgc cggcgccccg
    10561 cccggatccg tggcgcccgt ggccagcaac ccaagggagg ccagcgccgc cgcgagcgtg
    10621 ccgaggcgcc agccgagcag cagcacgacg gccggcgtgc cgagcacgtg caatgcaagg
    10681 cccggatcaa cgcgcgcatg gatgctccac aacagcgcca gcgccaccgc ggaaccgagc
    10741 agcaggtgga gccgctcagg gtggccgcgc aaagcgcgca agcccggcaa ggctgcaagg
    10801 aagagaaggc cggccagcac agcgcaggcg agcctgagcc cgggggaaag cacctcggcg
    10861 aggatctgca ttgcggggcg gcggacttca caggagcctg gatcgggact ccaattctac
    10921 gcgccggctg ccgcggccgg catcgagtga gcggctccgg cgtcgcatcg ctgcgcgacc
    10981 ggagccgccc cggcgcctca gtggctgtgt gccgcgaact gcccgtcgcc gctctcgagg
    11041 cggatgctga cgcgccgttg cagtgcccag ccgtcaggat cggcggaaac ctccggcgcc
    11101 agcgccgcgg gggcgccatg cgccgtgagg cggatgcgct cgggtgcaat gcctgcctcg
    11161 accaggcggt cacgtacggc ctcggctcgc gctgccgaca gattcaggtt gtgacgctcg
    11221 gtgccacgcg ggtcggcgta gccctcgagg tgcaccttca tctccggcgc cgcctgcaac
    11281 agcccggcca gcgcctcaag tgcatcggat gcgccgtccg gcaggcttgc actgtcggtg
    11341 cggaaaccga cctccagctc caggccctgc gccagcagcc cggcgtccac gcggctctcg
    11401 gccagcgcct ggagctcgcc gaggcgctgc gccaggtcgg ccaggcgggc cttctggctg
    11461 cgattgtcgg attgcaggct gtcgcgctcg accgtcagcg cagcgacgct ttgcgacaac
    11521 tcgccgttgc gccggatctt ctgttcgcgg tcgacggtga cgccgcctac ggccatgccg
    11581 gcgatggcgc ccggcggacc gccgacgatg gcgccgagca cgccgcctgc aatgatgccg
    11641 at
//
LOCUS       DAOMPG010000053        11622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2386_length_11622_cov_4.032247, whole genome shotgun sequence.
ACCESSION   DAOMPG010000053 DAOMPG010000000
VERSION     DAOMPG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_11622_cov_4.032247"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLS34_04430"
     CDS             <1..851
                     /locus_tag="PLS34_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HRP86751.1"
                     /translation="VHNGEQWLYFDPRDGSRGLPNNLLIWSWSSTPVLEVEGGRGAVL
                     GLSMRHTIEDALRVAELRAQLKHPRILEFSLLDLPLQTQSLYSILLLVPIGAFLVVVL
                     RNVVGISTFGTFMPVLVAMAFRETKLLNGIVLFLLIVAVGLSIRFYLERLRLLLVPRL
                     AAVLTVVVLLMGVVSVMSHRLGMEVGLSVALFPMVIIAMTIERMSIIWEERGPAEALK
                     EGFGTLVAAALIYVVMITPEVRHFVLVFPEVLLVLLAGILLLGRYTGYRLTELTRFRA
                     LAGGRK"
     gene            851..1819
                     /locus_tag="PLS34_04435"
     CDS             851..1819
                     /locus_tag="PLS34_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HRP86752.1"
                     /translation="MWRRWRALNEAGVMGINRRNSEYIMALNPRRNYPIVDNKLLTKE
                     LAIQHGVPVPELYGAIATPHDIRNLPAIVAERQDFVLKPAHGSQGDGIIVIVDRRKGR
                     YRTSGGRLLDWDELEHHLTNTLSGQFSLGGLPDKVMVEYRVRFDPVFEEVSFQGVPDL
                     RVIVYRGFPVMSMVRLPTRQSDGKANLHQGAVGSGIEIATGRTLPAVGAGNVVVDEHP
                     DTGAAIAGRIIPHWKQILLMAARCHELTGLGYLGADIVLDANRGPLLLELNARPGLNV
                     QIANQDGLRRRIKAVDPLAERSHSPEERVALARELARHGFRGARAA"
     gene            complement(1832..2677)
                     /locus_tag="PLS34_04440"
     CDS             complement(1832..2677)
                     /locus_tag="PLS34_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRP86753.1"
                     /translation="MSDTSTGKRADTVIVERRGPTTLVVLDRPAARNAVDPATARALY
                     DAMLAFEADPDAAVAVLAGRGESFCSGFDLKALAAGETDAWLARTHFGESGEPPLGPM
                     GPTRLRLTKPVIAAIAGPAVAGGMELALWCDLRIMERSAWMGVLCRRWGVPLIDGGTV
                     RLPRLVGLGRALDLAPARRALDLILTGRRVNADECLAIGLCERIAEPGTALEAALQLA
                     ADFAALPQVCLRSDRGAARYGALGDEAAALQAEYLAGLAALRMEAHDGAARFAGGAGR
                     HGAPA"
     gene            2942..4711
                     /locus_tag="PLS34_04445"
     CDS             2942..4711
                     /locus_tag="PLS34_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HRP86754.1"
                     /translation="MCGIAGEIRFDGAAADPGRVQRMLDLMSARGPDDDGLVAQGAVA
                     LGHRRLAIVDLSERGAQPMRDQELGLTIVFNGCIYNHDELRVRLARQGYRFFSRSDTE
                     VILKAYHAWGEACVDEFKGMFAFCIRNNTSGRLFLARDRLGIKPLYYTRDDKVFRFAS
                     TLPALTRDGLSSHALDPAALHHYMSFHSVVPAPRTLLQAVRKVPPGCWLRIEPDGRIT
                     EQRYWALGFGANPADPQTDAEWEDAVLEAMRVAVRRRLTGDVPVGVLLSGGLDSSLIT
                     GLLAGEGVPHLNTFSVGFEDVGKEEGNEFRYSDLVARTFGTEHHQIFVDSSTLLQHLP
                     GCFSAMSEPMVSHDNIGFYLLSREVSKSIKVVQSGQGADEVFGGYHWYPPLLESLDPV
                     GDYARVFFDRDHAEFAAAVEPELVGEDHSRDFVAAYFDEIQAERPVDKALHIDTTVML
                     VDDPVKRVDNMTMAWGLEARTPFLDHEVVELAARCPARLKLAQEGKGVLKEAARRVIP
                     HGVIDRPKGYFPVPALKYLRGDSLAMVRDALSGRAARERGVFRRDYVERLLAAPEEHL
                     TPLRGSKLWQLAVFELWMQTHGL"
     gene            4716..6530
                     /gene="ngg"
                     /locus_tag="PLS34_04450"
     CDS             4716..6530
                     /gene="ngg"
                     /locus_tag="PLS34_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HRP86755.1"
                     /translation="MSGKKPTGSDKHPTRATASQSDRSRHRLDRQGSATLRGFGEGDP
                     QHKGTPLKPNAVVDCGWGRLVFAHTFGDNAELARTLLEEAPGQRDIGLYLRDPHVVLA
                     MAPQELFLDPSHTYRLWFFKYLPARVQPRGFTVRRLRDRRDAEAMHRLLSARRMVPPS
                     PDFVMARRKSRTLSFFVAEEEDSGRIIGTVTGVDHVHAFGDPEKGSSMWCLAVDPQAV
                     QPGIGRALVAWIADHYAARGRAFMDLSVMHDNQAVIRLYEKMGFERVPVFSLKRKNAI
                     NEPLFVGPDNYDTLNPYARIIVDEARRRGVGVELVDAEEGYFALTLGGRSIICRESLS
                     ELTSAVAMSRCDDKRITRKVLAGAGLRVPAQRIAGEADENAAFLAKHGRIVVKPARGE
                     QGIGVAVGVSNEQEMQQAIAAASRSCERVLLESFAEGEDLRIVVIDYQVVAAALRRPA
                     EITGDGRLDIGTLIEKQSRRRAAATGGESRIPMDDETVRCLEKRGFTPADVLPAGRTL
                     RVRETANLHTGGTLHDVTDRLSPTLRDVAERAARALGIPVVGLDLMVPDPAGDEHVFI
                     EANERPGLANHEPQPTAERFLDFLFPQTVQVSDDPTGD"
     gene            6511..7629
                     /locus_tag="PLS34_04455"
     CDS             6511..7629
                     /locus_tag="PLS34_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HRP86756.1"
                     /translation="MTPLEIDLDYLQEVLVELLRIPSPAGFTDEAVRYTLRRLEELEI
                     PAEITRRGAIRADLKGAQGSPDRAIVTHLDTLGAITSDLKDNGRLELMPIGTWSSRFA
                     EGARVTVYTDHDSYRGTILPVKASGHAYNLEIDSLPVSWDSVELRLDEECFTRGDLVR
                     LGLNRGDFVAVDAQPEITPSGFINSRHLDDKGGVATVLAVAKALVESKTPLPVDCHLL
                     FTISEEVGSGASAVLHGDVAEMVTVDTGPVAPVQAGDEFGVTICMKDSAGPFDYHLTH
                     KLLGLCRDYGIRHQRDVFRHYKCDSASAIEAGNDIRTALVCFGTDATHGYERCHLRAL
                     HALAELLGLYVQSPPTARRDRLGLGSPEGFTHLPVSGT"
     gene            7713..8489
                     /locus_tag="PLS34_04460"
     CDS             7713..8489
                     /locus_tag="PLS34_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86757.1"
                     /translation="MQSRVFITGAASGIGRATARLFAARGWQVGICDRDAAALAAVRQ
                     ELGTDAARVYPCDVLDADALRAALIDFCGGAAAGLDLLVNNAGVLRVGPFEEMPPADH
                     RQLIEVNSRGVVEVLLAAFPMLQGARGRVVNLSSASAEYGTPDFATYAASKMFVRGLS
                     EALDIEWRRHGIRIACIMPAFVATPMIAGRRTHAMDRLGVELTAEDVAEVVWRAAHGR
                     RLMWHVGTRYRWLRALSKPLPGGIKRAIMRRISGYPGRPQ"
     gene            8486..9328
                     /locus_tag="PLS34_04465"
     CDS             8486..9328
                     /locus_tag="PLS34_04465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86758.1"
                     /translation="MNDAALRRAYAGKAVFITGAASGIGLGLAVRLAAAGARVRLSDR
                     DGPAGESAAAALRGAGYDAIAVRLDVRDADAFESAFAAAWRDMGQVDLLFNNAGIGAA
                     ADARDMRPELWRQVVEINLLGVMNGCQAAWQRMADAGGGRIVNVASAFGLLPGPLYAA
                     YSATKHGVVGLSRSLRAEGRALGIGVTAVCPGFIHTRILDNALMVGVDRDTAEAAVPF
                     RFISVEAAVGRILGGVARDRALVVFPWEMRLLWWLDRLAPALLDRVSAAAAKRYRAAA
                     GRPN"
     gene            9416..10771
                     /locus_tag="PLS34_04470"
     CDS             9416..10771
                     /locus_tag="PLS34_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRP86759.1"
                     /translation="MPIRIRRGLDLPIQGEPQQTIETARPVASVAVLGRDYLDLRPSM
                     HVQEGDRVKLGQPLFSDRRRPGVTITSPGAGIVSTVRRGARRALQSVIIRLEGEEEET
                     FDRWSAGELAGLDRDLVVENLTRSGLWCALRTRPWSKVPDPATTPHALFINAMDTNPL
                     APDPVVVVGEAAEAFSNGVAVLSRLTPGATFVCKATDAPLPVPTDVATLQVAEFTGPH
                     PAGLVGTHIHFLYPVGADRTVWHLGYQDVIAIGRLFTTGRIDPTRVVSLAGPMVKNPR
                     LLRTRLGAHIEPLVTGEIQDDIEGRVLSGSVWSGYRAVGWAGFLGRYHQQVTVLAEGR
                     QRELIGWLRPGGEKVSVTRAFLSSFSPRTRRFALTTSQNGSPRAMVPIGSYERVMPLD
                     ILPTQLLRALVVGDTDEAQRLGALELDEEDLALCTFVCPGKYDYGPVLRERLDQIEKE
                     G"
     gene            10771..>11622
                     /locus_tag="PLS34_04475"
     CDS             10771..>11622
                     /locus_tag="PLS34_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRP86760.1"
                     /translation="MAGLRKMLDRIEPMFSRGGRFEKLGALYEMVDTFLYTPRDVTPS
                     APHVRDAIDLKRVMTYVVIAVLPCALFGMYNIGLQANLAMAEMGVAAAEGWRGSVLAA
                     MGIGYDPDSVVACFWHGFLYFLPVYIVTLAAGGFWEVVFAITRNHEVNEGFLVTSMLY
                     TLILPPTIPLWQVALGISFAVVIGKEVFGGTGKNFLNPALTGRAFLYFAYPVQNSGND
                     VWVAVDGFSGATPLALAPQGGLEAIEAAGYTLGDMWMGFLPGTIGAESALACLIGAVF
                     LLYTRVAS"
BASE COUNT         1741 a         3935 c         4048 g         1898 t
ORIGIN      
        1 tggtccacaa cggcgaacag tggctgtatt tcgatccgcg cgacggcagc cgtggactgc
       61 cgaacaacct gctgatctgg tcatggagca gcactccggt actggaggtc gagggcggcc
      121 gcggcgccgt gctcggcttg tcgatgcgcc acaccatcga ggatgcgctg cgagtcgcgg
      181 agctgcgcgc ccagctcaag catccgcgca tcctggaatt ttcgctgctt gacctgccct
      241 tgcagacgca gtcgctgtac tcgatcctgc tgctcgtacc gatcggtgcc ttcctggtcg
      301 tagtgctgcg caacgtcgtc ggcatcagca ccttcggcac cttcatgccg gtgctggtgg
      361 cgatggcctt ccgcgaaacg aaactcctga acgggatcgt gctctttctg ctgatcgtcg
      421 cggtcggact gtcgatccgc ttctaccttg agcggctgag gctgctgctg gtcccgcggc
      481 tcgcggcggt actgacggtg gtggtgctgc tgatgggcgt ggtcagcgtg atgagccacc
      541 ggctgggcat ggaagtcggt ctgtcggtgg cgctgttccc catggtcatc atcgcgatga
      601 ccatcgagcg tatgtcgatc atctgggagg agcgcgggcc ggccgaggcg ctgaaggagg
      661 gcttcggcac gctggtggcg gccgcgttga tctacgtcgt gatgattacg cctgaggtac
      721 gccacttcgt actggtgttc cccgaggtcc tgctggtgct gctggccggg atcctgctgc
      781 tgggccgcta taccggctat cgcctcaccg agctgacgcg cttccgggcg ctggcgggag
      841 gacgcaagtg atctggcgtc gctggcgcgc gctgaacgag gccggcgtga tgggaatcaa
      901 ccggcgcaac tccgagtaca tcatggcgct caacccacgg cgcaattacc ccatcgtcga
      961 caacaagctg ctcaccaagg agctggccat ccagcacggc gtacccgtgc cggagctgta
     1021 cggcgcgatc gccacgccgc acgacatccg caacctgccg gccatcgtgg ccgagcgcca
     1081 ggacttcgtg ctcaagccgg cacacggcag ccagggcgac ggcatcatcg tcatcgtcga
     1141 tcgccgcaag ggtcgctacc gcaccagcgg cggccgttta cttgactggg acgagctgga
     1201 acaccacctc accaacaccc tgagcgggca gttcagcctc ggcgggttgc ccgacaaggt
     1261 catggtggaa taccgcgtac ggttcgaccc cgtgttcgag gaggtcagct tccagggggt
     1321 gcccgacctg cgcgtgatcg tctaccgggg tttcccggtg atgtcgatgg ttcgcctgcc
     1381 cacccgccag tcggacggca aggccaacct gcaccagggg gcagtcggct caggcataga
     1441 gattgccacc ggaagaacgc tgccggcggt cggcgccggc aacgtggtgg tcgacgagca
     1501 tcccgacacc ggcgccgcca tcgccggccg tatcattccc cactggaaac agatcctgct
     1561 gatggcggcg cgctgtcacg agctcaccgg cctcgggtat ctgggcgcgg acatcgtgct
     1621 ggacgcgaac cgcgggccgt tgttgctgga actcaacgcg cgacccggac tgaacgtgca
     1681 gatcgcgaac caggacggcc tgcgccggcg catcaaggcg gtcgaccccc tggcggagcg
     1741 ctcccactct cccgaggagc gcgtcgcact ggcgcgggag ctcgcgcgcc acgggtttcg
     1801 cggcgctcgc gccgcctgat tcacgcccgg ctcaggccgg tgcgccatgc cggccggccc
     1861 cgccggcgaa tcgagctgct ccgtcatgcg cctccatgcg cagtgctgcc agtcccgcca
     1921 ggtattcggc ctgcagtgcg gcggcctcgt cgccgagggc gccgtagcgc gccgcgccgc
     1981 gatcgctgcg caggcagacc tgcggcaacg ctgcaaagtc tgcggccagt tgcagtgcgg
     2041 cctcgagcgc agttcccggc tcagcgatgc gttcgcacag gccgatggcg agacattcgt
     2101 ccgcgttcac ccgccggccg gtcaggatga ggtccagggc gcgacgggca ggcgccaggt
     2161 ccagggcgcg gccgaggccg acgagacgcg gcaggcgcac cgtgccgccg tcgatcagcg
     2221 gcacgcccca gcgcctgcac agcacgccca tccaggcgct gcgctccatg atgcgcagat
     2281 cgcaccacaa ggcgagttcc atgccaccgg ccacggcggg cccggcgatc gcggcgatca
     2341 ccggcttggt gagccgtagg cgcgtggggc ccatcggtcc gagcggcggc tcaccgcttt
     2401 cgccgaagtg cgtccgcgcc agccaggcgt cggtttcgcc ggcggcgagc gccttgaggt
     2461 cgaacccgct gcagaaggac tcccccctgc cggcgagcac tgcgacggcc gcatcgggat
     2521 cggcctcgaa ggcgagcatg gcgtcgtaga gtgcgcgcgc ggtggcggga tcgaccgcgt
     2581 tgcgcgccgc gggacgatcg aggacgacga gcgtggtcgg gccgcgacgc tcgacgatga
     2641 cagtgtctgc gcgttttccc gtgctggtgt cagacatgct cgcaggtccg cgatttggga
     2701 gcgaagtacc gcgcatgtta cccttaatcg actgaatatc acgacctgga gaccgatgcc
     2761 cgcgcggcgc cgaagccgat catgcgtgcc ggaccagact gcccgcaggt cgccgcgatc
     2821 cgcccggatc gccattttcg cccactttgg ggcggagccg ccggggcgag tcaatgattc
     2881 attttgacgt gttcgagtgc agtgcgcgac cgcgaggccc gcgcccagag gagttttctt
     2941 gatgtgtgga atcgccggcg agattcgctt cgacggcgct gcggccgatc ccggccgcgt
     3001 gcaacggatg ctggacctga tgagtgcacg aggcccggac gatgacggcc tcgtggcgca
     3061 gggcgcggtg gcgctgggcc accggcgcct cgccatcgtg gacctctccg agcgcggcgc
     3121 gcagccgatg cgcgaccagg agctcggcct gacgatcgtc ttcaacggct gcatctacaa
     3181 tcacgacgag ctgcgcgtcc gcctcgcccg gcagggctat cgctttttct cacgcagcga
     3241 caccgaggtc atcctcaagg cctaccacgc ctggggcgag gcctgcgtcg atgagttcaa
     3301 gggcatgttc gccttctgta tccgcaacaa caccagcggg cggctgttcc tggcgcgcga
     3361 ccggctgggc atcaagccgc tgtactacac gcgcgacgac aaggtgttcc gcttcgcttc
     3421 gacgctgccg gcgctgaccc gggacggttt gtcctcacat gcactcgacc cggccgcatt
     3481 gcatcactac atgagcttcc attccgtcgt gcccgcaccc cgcaccttgc tgcaggccgt
     3541 gcgcaaggtg ccgccgggct gctggctgcg catcgagccc gacggccgga tcaccgagca
     3601 gcgctactgg gcgttgggct tcggcgcgaa tccggccgac ccgcagaccg acgcggagtg
     3661 ggaggacgcc gtgctggagg cgatgcgggt cgccgtgcgc cgtcgcctga cgggggacgt
     3721 gccggtcggt gtgctgctgt ccggcggcct tgattccagc ctgatcacgg ggctgctggc
     3781 aggtgaaggg gtgccgcacc tcaacacgtt ttcggtcggc ttcgaggacg tcggcaagga
     3841 agagggcaac gagtttcgct actcggacct ggtggcgcgc accttcggca cggagcacca
     3901 ccagattttt gtcgactcct cgaccctgct gcagcacctg cccggctgct tctccgccat
     3961 gtccgaaccg atggtcagtc acgacaacat cggcttctac ctgctttcgc gcgaggtgtc
     4021 gaagtccatc aaggtggtgc agagcgggca gggcgccgac gaggtgttcg gcggctatca
     4081 ctggtacccg ccgctgctcg agagcctcga cccggtcggc gactacgccc gggtgttctt
     4141 cgaccgtgac catgcggagt tcgccgcggc ggtcgagccg gagctggtgg gcgaggacca
     4201 cagccgggat ttcgtggcgg cctacttcga cgaaatccag gccgagcggc cggtcgacaa
     4261 ggcgctgcac atcgacacga ccgtcatgct ggtcgacgat cccgtcaagc gcgtcgacaa
     4321 catgaccatg gcctggggac tggaggcgcg cacgccgttc ctcgaccacg aagtcgtgga
     4381 gctggcggcg cgttgcccgg cccgcctcaa gctggcgcag gagggcaaag gcgtgctcaa
     4441 ggaggctgcg cgccgggtca tcccgcatgg ggtcatcgac cgtcccaagg gctatttccc
     4501 ggtgccggcc ctcaagtacc tgcgcggcga cagcctcgcc atggtgcgcg acgcattgtc
     4561 cgggcgcgcg gcgcgtgagc gcggcgtgtt ccggcgcgat tatgtcgagc gacttctggc
     4621 ggcgcccgaa gagcacctca cgccgctgcg cggttcgaag ctctggcagc tcgctgtatt
     4681 cgagctgtgg atgcagaccc acggactgtg aggcgatgag cggcaagaag cccacgggca
     4741 gcgacaagca tccgacacgg gcgaccgcgt cgcagtcgga ccggtcacgc catcgactgg
     4801 accgacaggg atcggccacc ctgcgcggct tcggcgaggg cgatccgcag cacaagggaa
     4861 cgcccctgaa gcccaacgcc gtggtcgatt gcggctgggg gcggctggtg ttcgcgcaca
     4921 ctttcggcga caacgccgag cttgccagga cattgctgga agaagcgccg ggccagcgcg
     4981 acatcggact ttacctgcgc gacccgcacg tcgtgcttgc gatggcgccg caggagctgt
     5041 tcctcgatcc ctcgcatacc taccggctgt ggttcttcaa gtacctgccg gcgcgtgtcc
     5101 agccgcgcgg cttcaccgtg cgccggctgc gcgatcgccg cgatgccgag gccatgcatc
     5161 gcctgctgag cgcccggcgg atggtgccgc ccagcccgga cttcgtgatg gcgcgccgca
     5221 agtcgaggac gctgtccttt ttcgttgccg aggaggagga cagcggccgg atcatcggga
     5281 ccgtcacggg cgtggaccac gtgcacgctt tcggcgatcc ggaaaagggc tccagcatgt
     5341 ggtgcctggc ggtggacccg caggccgtcc agccgggcat cggccgcgcg ctggtggcct
     5401 ggattgcgga tcattacgcc gctcgcggcc gcgcattcat ggacctgtcg gtgatgcatg
     5461 acaaccaggc ggtcatccgt ctgtacgaga agatgggctt cgagcgtgtc ccggtgttct
     5521 cactgaaacg caagaacgcc atcaacgagc cgctgttcgt cggcccggac aactacgaca
     5581 ccctcaatcc ctacgcccgc atcatcgtcg acgaggcgcg tcgccgcggt gtcggcgtcg
     5641 agctcgtgga cgccgaggaa gggtacttcg ccctcacgct cggcggacgc agcatcatct
     5701 gccgagaatc gctcagcgag ctcaccagcg ccgtcgccat gagccgttgc gacgacaagc
     5761 gcatcacgcg caaggtgctg gccggcgccg ggctccgggt gccggcgcag cgcatcgccg
     5821 gggaggccga tgagaatgcc gcgttcctgg cgaagcacgg ccggatcgtg gtcaagccgg
     5881 cgcgaggcga gcagggcatc ggcgtggcgg tcggcgtgag caacgagcag gagatgcagc
     5941 aggcgatcgc tgccgcgtcg cggagctgcg agcgcgtgtt gctggagtcc tttgccgagg
     6001 gcgaggacct gcgcatcgtc gtcatcgact accaggtcgt ggcggcggcg ctgcgccgac
     6061 ccgcggagat caccggcgac ggtcggctgg atatcggcac tctgatcgag aagcagagcc
     6121 ggcggcgcgc ggcggccacc gggggcgaga gccgtattcc catggacgac gaaacggtac
     6181 gctgcctgga gaagcggggc ttcacgccgg ccgacgtgct gccggcaggc cggacgctgc
     6241 gggtgcggga gaccgccaac ctccacaccg gtgggacact gcacgacgtg accgaccggc
     6301 tttcgccgac cttgcgcgac gtcgccgagc gggcggcgcg cgcgcttggt atcccggtcg
     6361 tcggcctgga cctgatggtg ccggatcccg ccggcgacga gcacgtgttc atcgaggcca
     6421 acgaacggcc gggcctggcc aaccacgagc cgcagccgac cgccgagcgc tttctcgatt
     6481 tcctgtttcc acagaccgta caggtgagcg atgaccccac tggagattga cctcgactac
     6541 ctgcaggaag tgctggtaga gctgctgcgg atcccgagcc cggccggctt cacggacgag
     6601 gcggtgcgct atacgctgcg ccggctcgag gagctggaga tcccggccga gatcacgcgg
     6661 cggggcgcca ttcgtgccga cctgaagggt gcgcagggca gtccggaccg cgccatcgtc
     6721 acccacctgg acacgctcgg cgccatcacc tccgacctga aggacaacgg ccggctggag
     6781 ctgatgccga tcggcacctg gtcgagccgc ttcgccgagg gcgcccgcgt cacggtctac
     6841 accgatcacg acagctatcg cggcaccatc ctgccggtga aggcgtcggg ccacgcctac
     6901 aacctggaga tcgacagcct gccggtgagc tgggactcgg tcgaactgcg cctcgacgag
     6961 gaatgcttca cccgcggcga cctggtgcgt ctcggcctga accggggcga tttcgtcgcc
     7021 gtcgacgcgc agccggagat cacgccctcg ggcttcatca actcgcgaca cctggatgac
     7081 aagggcggcg tggctacggt cctggcggtg gccaaggccc tcgtcgagag caagacgccg
     7141 ctgccggtcg attgccacct gctgttcacc atcagcgagg aggtgggctc cggcgcctcg
     7201 gcggtgctgc acggcgacgt cgcagaaatg gtcacggtcg acacggggcc ggtggcaccg
     7261 gtgcaggcgg gcgacgagtt cggcgtcacc atctgcatga aggattccgc cgggcccttc
     7321 gattatcacc tgacccacaa gctgctcggg ctgtgcaggg attatggtat ccggcaccag
     7381 cgcgacgtgt ttcgccatta caagtgcgac agcgcctccg cgatcgaggc cggcaacgac
     7441 atccgtaccg cgctagtgtg tttcggcacc gacgcgaccc atggttacga gcgctgtcac
     7501 ctgcgcgccc tgcatgcgct cgccgaactg ctcggcctgt acgtgcagag cccgcccacc
     7561 gcgcgccgcg accgcctcgg tctcggctcg ccggagggct tcacgcacct gccggtcagc
     7621 ggcacctgaa gcgggccgcg ccccgtgcct cgcggcgctt cacgtgcgcc gcgccgaacg
     7681 ctaagctgcc cgccttggcg ggggggcgag gcatgcaatc cagggtcttc atcaccggtg
     7741 cagcgtccgg catcggccgc gcgaccgcgc ggctgttcgc cgcacgtggc tggcaagtgg
     7801 gaatctgcga ccgggatgcg gcagcgctgg cggccgtccg gcaggaactc ggcaccgacg
     7861 ccgcccgggt ctatccctgc gacgtactcg acgccgacgc gctgcgggcg gcactgatcg
     7921 acttttgcgg cggcgcggct gccgggctgg acctgctcgt caataacgca ggcgtgctca
     7981 gggtcgggcc cttcgaggag atgccgccag cagatcatcg ccagctgatc gaggtgaact
     8041 cgcgcggcgt ggtcgaggtg ctgctggccg cctttccgat gctgcaaggc gcgcgtggac
     8101 gcgtggtgaa cctcagttcg gcctcggccg agtacggcac ccccgatttc gccacctacg
     8161 cggcgagcaa gatgttcgtc cgtggcctgt cggaggcgct ggacatcgag tggcggcggc
     8221 acggtatccg cattgcctgc atcatgccgg ctttcgtcgc cacgccgatg atcgccggcc
     8281 gccgcaccca tgccatggac cgcctgggcg tggagctgac ggccgaggac gtcgccgagg
     8341 tcgtgtggcg cgcggcgcac gggcgccgct tgatgtggca cgtcggcacg cggtaccgct
     8401 ggttgcgtgc cttgtcgaaa ccgcttcccg gcggcatcaa gcgcgcgatc atgcgccgga
     8461 tttccggcta tccggggagg ccgcagtgaa cgacgcagcg ctgcgtcgtg cctatgcagg
     8521 caaggcggtg ttcatcaccg gtgccgcctc cggcatcggc ctggggctcg ccgtccgcct
     8581 ggcggccgcc ggcgcgcgcg tccggctcag cgaccgggac ggccccgccg gcgaaagcgc
     8641 cgcggcggcg ctgcgcggcg ccggttacga tgccatcgcc gtacgcctcg acgtgcgcga
     8701 tgcggatgcc ttcgagtcgg ccttcgcggc ggcgtggcgc gacatggggc aggtggacct
     8761 gctgttcaac aatgccggca tcggcgccgc ggcggacgcg cgggacatga ggccggagct
     8821 ctggcggcag gtggtcgaga tcaatctgct gggcgtgatg aacggttgcc aggcggcctg
     8881 gcagcgcatg gcggatgccg gcggcggacg gatcgtcaac gttgcctccg ctttcgggct
     8941 cctgcccggg ccgctgtacg ccgcctacag cgcgaccaag cacggcgtgg tcgggctgtc
     9001 gcgctcactg cgcgcggagg gcagggcgct ggggatcggc gtgaccgcgg tctgtcccgg
     9061 gttcatccat acccggatcc tcgacaatgc cctgatggtg ggcgtcgacc gcgataccgc
     9121 cgaggcggcg gtgccgttcc gcttcatcag cgtcgaggcc gccgtcgggc ggatactcgg
     9181 cggcgtggcc cgcgaccgcg cgctggtggt gttcccgtgg gaaatgcgcc tgctttggtg
     9241 gctcgaccgg ctcgccccgg ccttgctgga ccgcgtcagt gcagcggccg cgaagcgcta
     9301 tcgcgcggcc gccgggcggc ccaactgagg cctcgtctcg ggtatacttc gtcacaatga
     9361 aaacaaggcg cgcaggcctc gctcgcgcgc cgcatcagcg attcgcggga actgcatgcc
     9421 gatcagaatt cgccgcggcc tggacctgcc gatccagggc gagccacagc agaccatcga
     9481 gaccgcccga cccgtcgcct cggtcgcggt cctggggcgt gattacctcg atctgaggcc
     9541 ctcgatgcac gtccaggaag gtgaccgggt caagctcggc cagcccttgt tcagcgaccg
     9601 gcggcggccc ggtgtcacca tcacttcgcc gggcgccggc atcgtgtcca ccgtgcggcg
     9661 cggcgcccgg cgcgcactgc agtcggtcat catccgcttg gagggcgaag aagaggaaac
     9721 cttcgaccgc tggtcggccg gcgagctggc ggggctggat cgtgacctgg tggtcgagaa
     9781 cctgacccgt tccggcctgt ggtgcgcact gcgcacgcgg ccgtggagca aggtgccgga
     9841 cccggctacc accccgcacg ccctgttcat caatgccatg gacaccaatc cgctggcgcc
     9901 ggacccggtc gtggtcgtcg gcgaggcggc cgaggccttc tcgaacggcg tggcggtgct
     9961 gtcgaggctg acgcccggcg cgaccttcgt ctgcaaggcg accgacgctc cgttaccggt
    10021 gccgacggac gtggcgacgc tgcaggtggc ggaattcacc ggcccgcatc cggccggcct
    10081 ggtcggcact cacattcatt tcctctaccc ggtgggcgca gaccgcactg tgtggcacct
    10141 gggttaccag gacgtcatcg ccatcggccg gctgttcacc acgggccgca tcgacccgac
    10201 tcgcgtggtg tcgctcgccg gtccgatggt gaaaaacccg cggctgctgc gcacgcggct
    10261 cggcgcccat atcgagccgc tggtgaccgg tgaaatccag gacgacatcg aggggcgggt
    10321 gctttccggt tccgtctgga gcggctaccg ggcggtcggc tgggccgggt tcctcgggcg
    10381 ctatcaccag caggtcaccg tgctggcgga aggccgccag cgggaactca tcggctggct
    10441 gcggcccggc ggcgagaagg tgtcggtgac gcgcgccttc ctgtcgagtt tcagcccgcg
    10501 cacgcgacgg tttgccctga ccaccagcca gaacggcagc ccgcgggcca tggtgccgat
    10561 cggttcctac gagcgcgtca tgccgctgga catactgccc acgcagctgt tgcgggcgct
    10621 ggtcgtaggc gacacggacg aggcccagcg cctcggggcg ctggagctcg acgaggaaga
    10681 cctggcgctt tgcaccttcg tgtgcccggg caagtacgac tacggcccgg tgctgcggga
    10741 gcgcctcgac cagatagaga aggaaggctg atggccggtt tgcgcaagat gctcgaccgg
    10801 atcgagccga tgttcagccg cggcggacgc ttcgagaagc tcggcgccct ctacgagatg
    10861 gtggacacct tcctgtacac gccgcgcgac gtcaccccga gcgcgccgca cgtgcgggat
    10921 gccatcgacc tcaagcgcgt catgacctat gtggtcattg cggttctgcc ctgcgcattg
    10981 ttcggcatgt acaacatcgg gctgcaggcc aatctcgcca tggcggagat gggcgtcgcg
    11041 gcggccgagg gctggcgcgg cagcgtgctg gccgccatgg gcatcggcta cgacccggac
    11101 agtgtcgtgg cctgtttctg gcacggcttc ctgtatttcc tgcccgtgta catcgtcacg
    11161 ctggcggccg gcgggttctg ggaggtggtg ttcgccatca cccgcaacca cgaggtcaac
    11221 gagggcttcc tcgtcacctc gatgctgtac acgctgatcc tcccgccgac cattccgctc
    11281 tggcaggtgg cgctcggcat cagcttcgcc gtggtcatcg gcaaggaggt ctttggcggt
    11341 accggcaaga acttcctcaa tcccgcgctc accggtcggg ccttccttta tttcgcctac
    11401 cctgtgcaga attccggcaa cgacgtctgg gtcgcggtgg acggcttcag cggcgcgacg
    11461 cccctcgcgc tggcgcccca gggcgggctc gaggccatcg aggcggcggg ttacacgctg
    11521 ggtgacatgt ggatgggctt cctgccgggc accatcggcg ccgagtcggc gctggcgtgc
    11581 ctcatcggcg ccgtattcct gctttacacc cgcgtcgcat cc
//
LOCUS       DAOMPG010000054        11604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2395_length_11604_cov_4.543510, whole genome shotgun sequence.
ACCESSION   DAOMPG010000054 DAOMPG010000000
VERSION     DAOMPG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2395_length_11604_cov_4.543510"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLS34_04480"
     CDS             complement(<1..1106)
                     /locus_tag="PLS34_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRP86761.1"
                     /translation="MQGGRRLLTLPAAAMLVAALVALPVVTVALHVFLPDQGAWRHIA
                     RVLLPEYIGNTLLLVAGTAFGVLALGLSTAWLVSVCRFPGRRLFEWALVLPLAFPAYV
                     IAYTYTDLLQVSGPVQTYIRDLTGWSARDYWFPPIRSLGGAVAMLSLVLYPYVYLLAR
                     AAFVQQSSGILEAARTLGCSPWGAFVKVALPMARPALVAGVLLALMETLADFGTVAYF
                     GVPVFTTGIYRAWFSLGDPVSAAKLSAVLLAFVLTLVALERWNRGGMRFHQLGAPAAE
                     RKPYELRGGPAALAVAVCMVPLAFGFLLPAGRLAWLSWIGGDAQFGARYLGLLWNSVS
                     LAGISAVVAVAAAVVVGYGLRLQPGRPMKLVHRIA"
     gene            complement(1119..2204)
                     /locus_tag="PLS34_04485"
     CDS             complement(1119..2204)
                     /locus_tag="PLS34_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP86762.1"
                     /translation="MVIPFQTPGVTMSRIRTVFARLSLVVLLTAIAAGCGRAPEESGA
                     GVINIYSARHYQSDDALYQAFTDATGIRVRRIEGTDDALIERILQEGAASPADILFTV
                     DAGRLWRAEQAGIFQPVESPILNQRIPAQLRHPEGKWFGFSSRARMIFFDTARIPADA
                     ITRYEDLADPRWVGELCIRSGGNIYNLSLMAAMIAHHGLEAAEDWARGVVANFARNPQ
                     GGDTDQLRAVAAGECGIAVANSYYFARLMTSEDPADRAVVEKLGWVFPNQQDRGTHVN
                     ISGAGIVTTAPNAAQALAFLEFLASPDAQTRFARGNNEYPVVAGAALENPALEALGAF
                     KADPIDATLYGVHQAEAQRALDRVGWK"
     gene            2321..3325
                     /gene="lipA"
                     /locus_tag="PLS34_04490"
     CDS             2321..3325
                     /gene="lipA"
                     /locus_tag="PLS34_04490"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRP86763.1"
                     /translation="MDSDFKGIPIVQPGAVPSGTKFRTEHGVTAIKNGVKQNRRAEPA
                     PVGRKPPWLRAKMPAGAGYSRVRENVREHRLATVCEESMCPNIGECWNNGTATLMIMG
                     SVCTRACRFCAVDTGNPRGWLDAEEPANAARSVQLMDLRYVVITSVDRDDLPDGGAAH
                     YAACVRAIKALNPDTAVEALTPDFRGELAAVETVVDSGLEVYAQNLETVRRLTHPVRD
                     PRAGYEQTLDVLEHAKRFRPDVLTKTSLMLGLGETDAEVRETLGELRERKVDIVTFGQ
                     YLRPTKNHLPVERFVTPEEFAEYRRIGLDMGFLEVVSGPLVRSSYRADRVFEQNNVGL
                     "
     gene            3322..4380
                     /locus_tag="PLS34_04495"
     CDS             3322..4380
                     /locus_tag="PLS34_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRP86764.1"
                     /translation="MTRIKGRGAASNREGRFESVRREAVDDGWFPEAPHEATRPPTTV
                     TEERAHGIVSRNASPDVPFEQSINPYRGCEHGCIYCYARPSHAYLNLSPGLDFETRLF
                     AKSNAAGLLRDTLAKRGYRPSPITLGANTDPYQPIEKRWRITRQLLELLAEARHPCTI
                     ITKSAMVERDLDLLAGLARDGLVAVLVSVTSLDNRLSAKLEPRASAPHRRLQAIRALS
                     AAGVPTGVLVSPVIPMVTDVDLEEILAQARTAGATQASYAIVRLPGEVAELFREWLEE
                     HLPERAAHVMSLVQQMRGGRDNDPRFGVRMKGEGVFAELIARRFKVATRRLGFLQGEA
                     YDLRCDLFRAPGPQGSLF"
     gene            4957..5730
                     /locus_tag="PLS34_04500"
     CDS             4957..5730
                     /locus_tag="PLS34_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86765.1"
                     /translation="MMIFRRPIVVSIIIASAISFGAYGIYLKRNELMATDSSQRHPLL
                     NHSRDAQQTTQTGLSSSDSDSVRLGDFPPTIESSPPLEETMQNSAADVEPFHRRESED
                     PGQTGDPVPEREATIGILLNSVVERLGVEGHHDLLQFILDEEIKETGLDVDAALSIEA
                     AISEIAAARGVSSGVGSGDSDIQCTASLCTLLVESGGPFQMTRMSPEFQAMMEQGPVS
                     MLWWVRLGTRERLDHVRLYMYRPGIEQHLNLLEAVVATP"
     gene            complement(6044..7066)
                     /locus_tag="PLS34_04505"
     CDS             complement(6044..7066)
                     /locus_tag="PLS34_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRP86766.1"
                     /translation="MDIVSSPEHHRQHGRAELTDGRLVPVFEHPGRIDAILESLARRG
                     FPSPALPEDRGVAPITAVHDLDYVAFLQCAWDEWAAVHGTDGDALPLSWRAPGMRTDR
                     EPERIDGRLGHWSFDAGTPITAGTWTAASGAAQAADTARRRVSAGARAAFALARPPGH
                     HAGSRFFGGYCFLNNAAIAAQGFREAGVERVAVLDVDYHHGNGTQQIFAGRADVFTAS
                     LHADPKHTFPFFLGHADETEGGTHLNVPLPRGTDWARYAPALDGVLGRIADEEPGALV
                     VSLGVDTWDGDPICSFALQTADYRQMGRRIGRLGLPTVIVMEGGYAVESLGDNVSEFL
                     EGFAEA"
     gene            7158..8147
                     /gene="sohB"
                     /locus_tag="PLS34_04510"
     CDS             7158..8147
                     /gene="sohB"
                     /locus_tag="PLS34_04510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HRP86767.1"
                     /translation="MTEALLEYVLFLAKTVTLVAAVTFVVGLVMNASRRTREGEGLKV
                     TNLNSRFRQAADGLRQALLPKAQRKQLAKQRKQEAKAEKERRRKRVFVIDFRGDIRAS
                     ASASLREEVSAVLAVAEQGDEVLVRLENAGGLVHEHGLAASQLARVRERGIPLTVAVD
                     KVAASGGYLMACIADRIIAAPFAIVGSIGVLAQIPNFRRMLDERGVTVEQVKAGRFKR
                     TVSMFGEVTDEDRDKLREELEEVHALFQAMVAKYRPALDLEKVATGEYWYGTRALELG
                     LVDELGTSDDWLTKLVGEADAWRVEWRGPKRLWEKLAAAMEEGSAGGWLQRWH"
     gene            8122..9222
                     /locus_tag="PLS34_04515"
     CDS             8122..9222
                     /locus_tag="PLS34_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRP86768.1"
                     /translation="MAGCSVGIERFAVVAACTLLSACGPNWVGFTRAPDAAAVVPWAP
                     LERSDLFESVSSETEVLGRPQKVVARHEDTFTDIARRFNLGYEELRAANPGVDPWIPG
                     EGTEILLPTAHVIPEAPREGVVVNLPAMRLWWFGPPDEHGRQTVITHPIGIGKIGWST
                     PLGSFKVLSSRKDPPWTPPASVRQAYAERGEHLPVVVPPGPDNPLGRHAVRLDNPVYL
                     LHGTNKPAGVGMRVSHGCIRLYPEDIERLFDDLPPGTPVHMVDQPWLAGWHDGQLHFE
                     VHPPLEDGNQDWNAALEQLRARARALDWERVATLGEAAMGLPLPVSAGSPDPARWLAA
                     APRVENRARGNFRDVEPEAEPAPEQLARDGRD"
     gene            complement(9240..10076)
                     /locus_tag="PLS34_04520"
     CDS             complement(9240..10076)
                     /locus_tag="PLS34_04520"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86769.1"
                     /translation="MKKNVLITGCSTGLGRSVALLLAASGWRVFAGVRKAADAKSLQA
                     EAEGELTPLLFDVARPDALAQAIETLRQATGGELHGLINNAGVYLGGPLELMKPEEIE
                     LTFAVNVTGLLAVTRGCLPMLRAAQGRIVNISSISGLIAMPGVSVYAGSKHAVEAITD
                     SLRIELHPFGVKVVAVEPGGIKTPIWEKGAKRDQEAADDAATAELRKAYAPLLKLLHK
                     LNARPGGLPPEDVAKVVIEALESDRPKNRYLVGKDARSLALLGRLPDALRDRAIIGKV
                     WR"
     gene            complement(10073..10555)
                     /locus_tag="PLS34_04525"
     CDS             complement(10073..10555)
                     /locus_tag="PLS34_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86770.1"
                     /translation="MQLIDDDEARADRRVFKVALADPSPLAGLDPQARVVAITIREDD
                     GEPSEECRGFCDCFIATAAWGSWLDPHVATLRGFRDGVLMQSAPGRAFVAFYYRHSPP
                     VAEIISRHESLRAATRALLAPLVFAIERPAVAGGLGLGLIGLLLGLNLRRRGARRLGP
                     "
     gene            complement(10546..11517)
                     /locus_tag="PLS34_04530"
     CDS             complement(10546..11517)
                     /locus_tag="PLS34_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRP86771.1"
                     /translation="MSRHHHSFPVLAMLLAALWAPAAGAAGVLQFLEPRLKATEGQTR
                     QITVIRSDSAVGEVTVVLNISLGGTATLGTDFTIDLPLGVIRIPDGRLFGRAQLNVPQ
                     RSGAQGTRFAILTLANPSGATLGRDVTLLLEIEDDDPPAAALEIAGDAVRRVVAGDEL
                     PIEVTRDGLPADTVSATLLGVPGTAALGIDFADLTTELEFAPGDTSADATLSTIARTA
                     AAPPRNLSLMLANTAPAGEAAFRTRPPTTPANSPCSPPTPRSTKTPAASRSRWTAIAA
                     ATARLPSAGSQWTATPARRRSQARTSRPPPAGWSSPTAKPARLSPCS"
BASE COUNT         1884 a         3863 c         4108 g         1749 t
ORIGIN      
        1 gcgatgcggt gcaccagctt catcggccgg ccgggctgca gccgcagccc gtagccgacc
       61 accaccgccg ccgccacggc gacgacggcg ctgatgccgg cgagacttac gctgttccac
      121 aagaggccga ggtagcgcgc accgaactgc gcgtcgccgc cgatccagct cagccaggcc
      181 agccgcccgg ccggcagcag gaagccgaag gccagcggca ccatgcagac cgccacagcg
      241 agcgccgcgg gcccgcccct gagttcgtag ggcttgcgct cagcggccgg tgcgccgagc
      301 tggtggaagc gcatgccgcc gcgattccag cgttccaacg caacgagagt cagcacgaag
      361 gcaagcagca ccgcggacag cttggccgcg gacaccgggt cgccgagcga gaaccaggcg
      421 cggtagatgc cggtagtgaa caccggcacg ccgaagtagg ccacggtgcc gaagtcggcg
      481 agcgtttcca tcagggcgag caacacgccg gcgaccagcg ccggccgcgc catcggcagc
      541 gccaccttga cgaacgcgcc ccaagggctg caaccgagcg tgcgcgccgc ctcgaggatg
      601 ccgctggatt gttgcacgaa ggcggcgcgc gccagcaggt agacgtaggg atacagcacc
      661 agcgacagca tcgcgaccgc gccgccgagc gaacggatcg gcgggaacca gtagtcgcgc
      721 gccgaccagc cggtcaggtc gcgaatgtaa gtctgcacgg gcccggagac ctgcagcagg
      781 tccgtgtagg tgtacgcgat gacgtaggcc ggaaaggcca gcggcagcac cagcgcccac
      841 tcgaacagcc gccggccggg gaagcggcac accgacacca gccaggccgt cgacagcccg
      901 agggcgagca caccgaaggc cgtgccggcc accagcagca gggtgttgcc gatgtattct
      961 ggcagcagca cgcgcgcgat gtgccgccag gcgccctggt cgggcaggaa aacgtgcaat
     1021 gcgacggtga cgaccggcag cgccaccagc gccgcgacca gcatcgcggc ggcgggcagc
     1081 gtcagcagtc ggcgaccgcc ctgcacgcgg ctggcggcct acttccagcc gacgcggtcg
     1141 agcgcccgct gggcctcggc ctggtgcacg ccgtataggg tggcgtcgat cgggtcggcc
     1201 ttgaaggcgc cgagcgcctc gagcgccggg ttctccaggg cggcgccggc caccaccggg
     1261 tactcgttgt tgccgcgggc gaagcgggtc tgcgcgtcgg gcgaggcgag gaactccagg
     1321 aaggccagtg cctgggccgc gttcggcgcg gtggtcacga tgccggcgcc ggaaatgttc
     1381 acatgggtgc cgcggtcctg ctggttgggg aacacccagc cgagcttctc gacgaccgcg
     1441 cgatcggccg ggtcctcgga ggtcatcagg cgcgcaaagt aatagctgtt ggcgacggcg
     1501 atgccgcact cgccggcggc caccgcgcgc agctggtcgg tgtcgccgcc ctgcgggttg
     1561 cgggcgaagt tggcgaccac gccgcgggcc cagtcttcgg ccgcctccag cccgtgatgg
     1621 gcgatcatcg cggccatcag cgacaggttg tagatgttgc cgccggagcg gatgcacagc
     1681 tcgcccaccc agcgcgggtc cgccaggtcc tcgtagcgcg tgatcgcatc ggccgggatg
     1741 cgcgcggtgt cgaaaaagat catgcgcgcg cgactggaga agccgaacca cttgccctcg
     1801 ggatggcgca gctgcgcggg aatccgctgg ttcaggatcg gcgactcgac gggctggaag
     1861 ataccggcct gctcggcgcg ccacaggcgg ccggcgtcga cggtgaacag gatatcggcg
     1921 gggctcgcgg cgccctcctg gagaatgcgc tcgatcagcg cgtcgtcggt gccctcgatg
     1981 cgccgcacgc ggatgccggt ggcgtcggtg aaggcctggt acagggcgtc gtcgctctgg
     2041 taatgccgcg ccgagtagat attgatgacg ccggcgccgg attcttccgg ggcgcggccg
     2101 cagccggcgg ctatggcggt taacaggacg accagtgaga gccgggcgaa gacggtgcgg
     2161 atgcgcgaca tggtgacccc gggggtctgg aaggggatga ccaatttaat gcgaatcgtt
     2221 cgcattagca accgtggcag gtcgtcagcg actccccggg cgggcgggcg gccgcgggcg
     2281 gttttcccgc aggccgggcg gcatgagaga atccggcgcc atggactccg atttcaaggg
     2341 cattccgatc gtccagccgg gggcggtgcc gagcggcacc aagttccgca ccgagcacgg
     2401 cgtgacggcg atcaagaacg gcgtgaagca gaaccgccgc gccgagccgg cgcccgtcgg
     2461 gcgcaagccg ccgtggctgc gcgccaagat gccggccggg gccggctact cgcgggtcag
     2521 ggagaacgtg cgtgagcatc gcctcgcgac cgtctgcgag gagtcgatgt gcccgaacat
     2581 cggcgagtgc tggaacaacg gcaccgcgac gctgatgatc atgggctcgg tttgcacgcg
     2641 cgcctgccgt ttctgtgccg tggataccgg caacccgcgc ggctggctcg atgccgagga
     2701 accggccaac gccgcgcgct cggtgcagct gatggacttg cgctacgtgg tgatcaccag
     2761 cgtcgaccgg gacgacctgc cggacggcgg cgccgcgcac tacgcagcct gcgtgcgtgc
     2821 catcaaggcg ctcaatccgg acacggccgt ggaggcgctg accccggatt tccgtggcga
     2881 gctcgccgct gtcgaaaccg tggtggactc ggggctggaa gtctatgccc agaacctcga
     2941 gaccgtgcgg cgcctgacgc acccggtgcg cgacccgcgc gccggctatg agcagacgct
     3001 ggacgtgctg gagcacgcca agcgttttcg tcccgacgtg ctcaccaaga ccagcctgat
     3061 gctgggcctc ggcgagaccg acgcggaagt ccgcgagacg ctcggcgagc tgcgcgagcg
     3121 caaggtcgac atcgtgacct tcggccagta cctgcggccg acgaagaacc acctgccggt
     3181 ggagcgtttc gtgacgccgg aggagttcgc tgagtaccgc cgcatcggcc tcgacatggg
     3241 attcctcgag gtcgtgtccg ggccgctggt gcgctcgagc taccgcgccg accgggtgtt
     3301 cgagcagaac aacgtcgggc tgtgacgcgc atcaagggcc gcggcgccgc ctcgaaccgc
     3361 gagggccgct tcgagtccgt gcgccgcgag gcggtcgacg acggctggtt tcccgaggcg
     3421 ccgcatgagg ccacgcgccc tcccacaacg gtcaccgagg aacgcgccca cggcatcgtc
     3481 tcgcgcaatg cctcgcccga cgtgcccttc gagcagtcga tcaatcccta ccgcggttgc
     3541 gagcacggct gcatctactg ctacgcgcgt cccagccatg cctacctgaa cctgtcgccg
     3601 ggcctagact tcgagacgcg gttgttcgcc aagagcaacg ccgccgggct gctgcgcgac
     3661 acgctcgcga agcgtggcta ccgaccgagc ccgatcacgc tgggcgcgaa caccgacccc
     3721 taccagccga tcgagaagcg ctggcgcatc acccgccagc tgctggagct gctcgccgag
     3781 gcgcgccatc cctgcaccat catcaccaag agcgccatgg tggagcgcga cctcgacctg
     3841 ctggccggcc tggcccggga cgggctcgtc gcggtgctgg tctcggtcac cagcctggac
     3901 aaccggttgt cggcgaagct cgagccgcgc gcctccgcgc cgcaccgccg gctgcaggcg
     3961 atccgcgcgc tgagcgcggc cggcgtgccg acgggcgtgc tggtctcgcc ggtgattccc
     4021 atggtcaccg acgtggacct cgaggagatc ctcgcgcagg cgcgcacggc cggcgccacg
     4081 caggccagct acgccatcgt gcgcctgccg ggcgaggtgg ccgagctgtt ccgcgaatgg
     4141 ctcgaggaac acctgccgga gcgcgccgcc cacgtcatga gcctggtgca gcagatgcgc
     4201 ggcggccgcg acaacgaccc gcgcttcggc gtgcgcatga agggcgaggg ggtgttcgcc
     4261 gaattgatcg cgcggcgctt caaggtggcg acccggcggc tcggcttcct gcagggcgag
     4321 gcgtacgacc tccgctgcga cctgttccgc gcgcctggcc cgcagggcag cctgttttga
     4381 gctgggcggc gccgagccag aaacccttcc gccgaacacg gcaattagag aggtctggcc
     4441 ccctggtgac gggtacctca tctaagtctt aggaatttca tgattactcc agggcaatct
     4501 ctatttgggc gcattcgccc atatatggta ttttcgtggc tgccgataat cattgtcgcc
     4561 tcaaccgcat aaggagctgc cagtgaatat gcatagaatc atgttgatgc ttgtcctgag
     4621 ctgtttatgg tgctcgcatg cgatagcgag cgacgacctc aacaatttca tttcaaatgg
     4681 caattcggag ggccactcta gttattggaa ctggaagcca acgacagacg gcggttggga
     4741 atacatcgct gcctggatga atcaagatgg cgaagtggtt tgggaaggca aatttgacac
     4801 ggacgggagc gagaagatca ccactcccaa gccacacgta ccgttccacg actggtcggg
     4861 ccaacatggt ttctcggttg gctcgggcaa ctggagtcac ccgtcatgct ttatgtttca
     4921 gatctgtgat ggcggaggcg ggtcctggga tccacagtga tgatatttcg caggcctatt
     4981 gttgtttcga tcatcatcgc atctgcgata tcattcggtg catacgggat ctatctcaag
     5041 cgcaacgaac ttatggcaac ggactcctca caaaggcatc cgttgttgaa tcattcccga
     5101 gatgcgcagc aaaccactca aaccgggctg tcttcatcgg attcggactc tgttcgcctt
     5161 ggggattttc ccccgacgat cgaatcctcc ccaccgctgg aagagaccat gcagaactct
     5221 gcagccgatg ttgagccttt ccacaggcgc gaatctgaag accccggcca aacgggcgat
     5281 ccagtaccgg agcgcgaagc aaccattggt attctgctta attcggtagt tgaacgcttg
     5341 ggtgtcgagg ggcaccatga cctgctccag ttcattcttg acgaggagat caaggagacc
     5401 ggcctggatg tcgacgccgc cctgtcgatt gaagccgcga tttcggaaat cgccgccgcc
     5461 cgtggggttt cgtctggcgt ggggtccggc gacagcgata ttcaatgcac agcgtcgctc
     5521 tgtacattgc tggtggaatc tggcggtccg tttcaaatga cgcggatgtc gccggagttt
     5581 caggcgatga tggagcaggg cccggtgtcc atgctctggt gggtaaggtt gggcactcgc
     5641 gagcgtctgg accacgtccg gctgtacatg tacaggcccg gcatcgagca acatttgaac
     5701 ctgctggagg ccgtagtggc cacaccataa catgcgcaaa taatgcgcca tgtgcgaata
     5761 cggcaacgaa aaccggaatc tcaggtgttt tcatccacct catggtcgca gccggttttt
     5821 ttcgatccgt gcgaccaaac ccctgcaacc ctttggccgc cagcggcatc tcagctagca
     5881 aacgaacaat gagcttgcag aggagagccg ccatgaaccg gatccaggaa ctgagctgcc
     5941 agatcgcgcg ccgcgagctc gttgtgccgc tcgacccacc gctgccccgc tggccgcggc
     6001 cgaagcggag ggcgagacgc atcgtccgtg ctgacggcgc ggctcaggcc tcggcgaagc
     6061 cctcgaggaa ctccgacacg ttgtcgccga gcgactcgac cgcgtaaccg ccttccatga
     6121 cgatgacggt cggcaggccg agccggccga tgcgccgtcc catctgccgg tagtcggccg
     6181 tctgcagggc gaagctgcag atggggtcgc cgtcccaggt gtccacgccc agcgacacca
     6241 ccagcgcgcc gggttcctcg tccgcgatgc gccccagcac gccgtccagc gcgggcgcat
     6301 agcgcgccca gtcggtgccg cgcggcagcg gcacgttcaa atgcgtgccg ccctcggtct
     6361 cgtcggcatg gccgaggaag aagggaaagg tgtgcttcgg gtcggcatgc agcgaggccg
     6421 tgaacacgtc tgcccgcccc gcgaagatct gctgcgtgcc gttgccgtgg tggtagtcca
     6481 cgtccagaac ggcgacccgc tcgacaccgg cctcgcgaaa gccctgcgcg gcaatggccg
     6541 cattgttgag gaagcagtag ccgccgaaga agcgactgcc ggcatggtgg ccgggcggcc
     6601 gcgccagggc gaaggccgcg cgcgcgccgg cgctgacgcg ccgccgggcc gtgtcggccg
     6661 cctgcgcggc gccgctggct gccgtccagg tgccggccgt gatgggcgtg ccggcgtcga
     6721 atgaccagtg gccgagccgg ccgtcgatgc gctccggctc acggtccgtg cgcatgccgg
     6781 gcgcgcgcca gctcagcggc agcgcgtcgc cgtcggtgcc gtgcaccgcc gcccactcgt
     6841 cccaggcgca ttgcaggaaa gcgacatagt ccaggtcgtg gaccgcggtg atcggcgcca
     6901 cgccgcggtc ctcgggcagc gccggcgaag ggaagccgcg ccgcgccagc gactcgagaa
     6961 ttgcatcgat tcggcccggg tgctcgaaca cgggcacgag ccggccgtcg gtgagctcgg
     7021 cccggccgtg ctggcggtgg tgctcgggac tggagacgat gtccatccgg ggacctgctt
     7081 gtgattggcc ggcgccccgg catgcgaggc tgcgctacca tagcgcgcaa cccggagacc
     7141 cggaaagaga gcctgtcgtg actgaggcat tgctggaata cgtgctgttt ctcgcgaaga
     7201 cggtgacgct ggtcgcggcg gtcaccttcg ttgtcgggct ggtcatgaat gcctcgcggc
     7261 gcacgcgcga gggcgaggga ctgaaggtca ccaacctgaa ttcccgcttc cgccaggcgg
     7321 ccgacggcct gcgccaggcg ctgctgccca aggcgcaacg caagcagctg gcgaagcagc
     7381 gcaagcagga ggccaaggcc gagaaagagc ggcgtcgcaa gcgcgtattc gtgatcgact
     7441 ttcgcggcga catccgcgcc agcgccagcg cctcgctgcg tgaggaggtc agcgccgtgc
     7501 tggccgtcgc cgagcagggc gacgaggtgc tggtgcggct ggagaatgcc ggcggcctgg
     7561 tgcatgagca cggcctggcg gcttcgcagc tggcccgcgt gcgcgagcgc ggcatcccgt
     7621 tgacggtggc ggtggacaag gtggcggcca gcggcggcta cctgatggcc tgcatcgccg
     7681 accgcatcat cgcggcgccc ttcgccatcg tcggctcgat cggcgtgctg gcgcagattc
     7741 ccaacttccg gcgcatgctc gacgagcgcg gcgtcaccgt cgagcaggtc aaggccgggc
     7801 gtttcaagcg gacggtgagc atgttcggcg aagtcaccga cgaggatcgc gacaagctgc
     7861 gcgaggagct cgaggaagtg cacgcgctgt tccaggcgat ggtcgcgaag taccgcccgg
     7921 cgttggacct ggagaaggtc gccaccggcg agtactggta cggcacgcgc gcgctggagc
     7981 tcgggctggt ggacgagctc ggcaccagcg acgactggct gacgaagctg gtgggcgagg
     8041 cggacgcctg gcgggtggag tggcgcggcc cgaagcgcct ttgggagaag cttgcggcgg
     8101 caatggagga gggaagtgcc ggtggctggc tgcagcgttg gcattgagcg gttcgcggtc
     8161 gtagcggcct gtacgctgct gtcggcctgc ggacccaact gggtcgggtt tacgcgcgcc
     8221 ccggacgcgg cagcggtggt gccctgggca ccgctggagc gcagcgacct gttcgagagc
     8281 gtcagcagcg agaccgaggt gctcggtcgc ccgcaaaagg tcgtcgcccg ccacgaggac
     8341 accttcaccg acatcgcgcg gcgcttcaac ctcggttacg aggagctgcg cgccgccaac
     8401 cccggcgtcg atccatggat cccgggcgag ggcaccgaaa tcctgctgcc gacggcacac
     8461 gttattccgg aggcgccgcg cgagggcgtg gtcgtcaacc tgccggccat gcgcctgtgg
     8521 tggttcggcc cgccggacga gcacggccgg cagacggtca tcacccatcc catcggcatc
     8581 ggcaagatcg gctggtcgac gccgctcggc agcttcaaag tactgtccag ccgcaaggac
     8641 ccgccgtgga ccccgccggc gtccgtgcgc caggcgtacg ccgaacgcgg cgagcacctg
     8701 ccggtggtgg tgccgcccgg gccggacaac ccgctgggcc ggcacgccgt gcggctcgac
     8761 aacccggttt acctgctgca cgggaccaac aagcccgccg gcgtgggcat gcgcgtcagc
     8821 cacggttgca tccggctcta cccggaggac atcgagcggc tgttcgatga cttgccgccg
     8881 ggtacgccgg tgcatatggt tgaccagccc tggctggccg gctggcacga cgggcagctc
     8941 cacttcgagg tgcacccgcc gctcgaggac ggcaaccagg actggaatgc ggccctcgaa
     9001 cagctgcgcg cgcgggcgcg cgcgctggac tgggagcgcg tggcgacgct cggcgaagcc
     9061 gccatggggc tgccgttgcc ggtgagcgcc ggcagcccgg acccggcgcg atggctggcc
     9121 gccgcgccgc gggtcgaaaa tcgcgcgcgc ggaaactttc gggacgtgga gccagaagcc
     9181 gaacccgcgc ccgagcaact cgcccgggac ggccgcgact agccgggcga cctccggggc
     9241 tagcgccaga ccttgccgat gatggcgcgg tcgcgcagcg catcgggcag gcggccgagc
     9301 agcgcgaggc tcctggcgtc cttgcccacc agatagcggt ttttcggccg atcggattcc
     9361 agcgcctcga tgaccacctt ggcgacgtct tccggcggca ggccgccggg cctggcattg
     9421 agcttgtgca gcagcttcag cagcggtgcg taggccttgc gtagctccgc ggtggctgcg
     9481 tcatcggcgg cctcctggtc ccgcttcgcg cccttctccc agatcggcgt cttgatgccg
     9541 cccggctcga ccgccacgac cttcacgccg aagggatgca gctcgatacg caaggaatcg
     9601 gtgatcgcct cgactgcatg cttggaaccg gcgtagacgc tcacccccgg catggcgatg
     9661 aggcccgaga tcgaactgat gttgacgatc cgcccctgtg cggcgcgcag catcggcagg
     9721 cagccgcgcg tcacggccag cagcccggtg acgttgacgg caaaggtgag ctcgatctcc
     9781 tcgggtttca tcagctccag cggcccgccg aggtacaccc cggcattgtt gatcaggccg
     9841 tgcagctctc cgccggtcgc ctggcgcagg gtctcgatcg cctgcgccag ggcgtccggc
     9901 cgggcgacgt cgaacagcag cggggtcagt tcgccctcgg cctccgcttg cagcgacttg
     9961 gcgtcggcgg ccttgcgcac cccggcgaac acgcgccagc ccgacgcggc gagcagcagc
    10021 gcgacgctgc gcccgaggcc ggtcgagcaa ccggtaatca gcacgttctt cttcatggcc
    10081 cgagtcttcg cgcgccgcgc cggcgcagat tcaggccgag cagcaggccg atcagcccca
    10141 gtccgagtcc gcctgcgacc gccggccgct cgatggcgaa gaccagtggg gcgagcaggg
    10201 cacgcgtcgc cgcgcgcaat gattcgtggc gggagatgat ttccgcgacc ggcggagaat
    10261 ggcgatagta gaaggccacg aaggcgcgcc ccggcgcgct ctgcatgagc accccgtcgc
    10321 ggaagccgcg cagggtggcg acgtgcggat ccagccagct gccccaggca gcggtcgcga
    10381 tgaagcaatc gcagaagccg cggcactcct cgctgggctc gccgtcgtcc tcgcggatgg
    10441 tgatcgcgac cacgcgggct tgcggatcca gcccggcgag cggcgaggga tcggcgagcg
    10501 ccaccttgaa cacccgccgg tcggcacgcg cctcgtcgtc gtcgatcagc tgcacggtga
    10561 aagtcttgcg ggtttcgccg tcggcgaact ccagccggcc ggtggcggcc gtgaagtccg
    10621 cgcctgcgac cgccgccggg ctggtgtcgc cgtccactgt gacccagctg acggcagccg
    10681 cgccgttgct gccgcgattg cggtccaccg tgaacgtgat gctgccggcg tcttcgttga
    10741 ccgtggtgtt ggtggtgaac agggagaatt cgccggcgtg gtcggcgggc gggtcctgaa
    10801 cgccgcctcg ccggccggcg cggtgttggc cagcatcagg ctcaggttgc gcggcggggc
    10861 ggccgccgtg cgcgcgatgg tcgacagcgt cgcatcagcc gaggtgtcgc cgggcgcgaa
    10921 ctccagctcg gtggtgaggt cggcaaaatc gatgcccagt gcggcggtac ccggcacgcc
    10981 gagcaatgtg gcggagacgg tatcggctgg cagcccgtct cgcgtgacct cgatgggcag
    11041 ctcgtcgccg gccaccaccc ggcgcacggc gtcgcccgcg atctcgagcg ccgcggccgg
    11101 cgggtcgtcg tcctcgatct ccagcagcag cgtgacgtcg cggccgaggg tcgcgccgct
    11161 ggggttcgcc agtgtcagga tggcgaagcg tgtgccctgg gcgccgctgc gctgcgggac
    11221 gttgagctgg gcgcggccga acagccggcc gtcgggaatc cggatcaccc cgagcggcag
    11281 gtcgatggtg aagtccgtgc cgagcgtcgc ggtgccgccc aggctgatgt tcagcaccac
    11341 ggtcacttcg ccgaccgcgc tgtcgctgcg gatcacggtg atctggcggg tctggccctc
    11401 ggtggccttg agccggggct cgagaaactg cagtaccccc gccgctcctg cggccggcgc
    11461 ccagagcgcg gccagcagca tggcgagtac ggggaatgag tggtggtgcc gagacatgat
    11521 ccatgctccc gcggtagtcg accggtgccg ctccatgatg ggacgacgga ggcattgggg
    11581 caatgcgggc ggagtctcag tcct
//
LOCUS       DAOMPG010000055        11511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2417_length_11511_cov_4.167161, whole genome shotgun sequence.
ACCESSION   DAOMPG010000055 DAOMPG010000000
VERSION     DAOMPG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2417_length_11511_cov_4.167161"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLS34_04535"
     CDS             <1..469
                     /locus_tag="PLS34_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRP86772.1"
                     /translation="ELAAPVDATSVRVRAPLDPGQRVGFMSWLENLTVAPGDPPARVI
                     VNSRTGTIVMGGNVKVLAAAVAHGGLSVTISESFAVSQPEPFSRGGETVVVPQSEVQV
                     TEGDGRMFPFGPGATLEEIVRAVNQVGAAPGDLIAILEALHQSGALRAELIII"
     gene            483..1487
                     /gene="flgJ"
                     /locus_tag="PLS34_04540"
     CDS             483..1487
                     /gene="flgJ"
                     /locus_tag="PLS34_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HRP86773.1"
                     /translation="MSLPAVTDTTATANLADLRRAARAGDASAAREAAKQFEALFVNM
                     MVKQMRDAMPTDGGLFGGEGMKLYESMHDQQLSLAMARRGGIGLADAIERQLLPPAAP
                     GASGEELRPLNFPAHRLPARVTPLVPLDPATAAAPSRPPPSPEEFVQRLLPHARRAAA
                     RLGVAPEVLIAQSALETGWGRHVMRDAAGRSSHNLFGIKASGGWSGASVRVSTLEYRD
                     GVARREQAAFRDYPDEAASFADYVRLVGGHPRYRQAVANADSPEGYLRGLQEAGYATD
                     PAYAEKILSILERGLPGLPGGDAAGAQFKFPGQRPPNNQTGDPEVVRTRPSPVGEEIS
                     "
     gene            1487..3145
                     /gene="flgK"
                     /locus_tag="PLS34_04545"
     CDS             1487..3145
                     /gene="flgK"
                     /locus_tag="PLS34_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRP86774.1"
                     /translation="MSGVFGIGTSALLAYQRALSVTGQNIANVGNENYSRQRIDLAVR
                     NPQFSGAGWYGQGVDVVGVQRIADQFVQRQVLDNTAGSARQQVYYQYAQRADNLLADA
                     EAGLAPVLQQFFAAVQDVANDPTSTAARQVLLSQGEALADRFAFLQRRLDDQRQVVEG
                     QIGAAVDEINGLAAGIASLNQKIAEGIGQSGGAMPNDLLDQRDAMIERLSGLVAVQTL
                     PQGDGTLNVMIGNGQNLVMGSTATVLTAQPLGADPSRTEIAVVNPGSTVGITDIISGG
                     ELGALLDVRRELLDPVQNALGRTAVGLALSFNELHRQGVDLDGVAGGDFFQLPQGEVL
                     VGRGNTATGQPALTFTDAAALTAGDYRVSFDGVEWRLTRTGSATPLATAAPGGTLAAD
                     GFELDLSGIAGATAGDSFDVRPTRTAAAGLGVAITDPRAFAAALPPAAPGESVVGDNR
                     NALALAGLASERVLANGTTSIAASYNELVANVGVKTRQAQLSAEAQGRMLEASRAQRE
                     SISGVNLDEEASNLLRYQQAYAAAAQVIATAGTMFDTLLMAVRR"
     gene            3150..4346
                     /gene="flgL"
                     /locus_tag="PLS34_04550"
     CDS             3150..4346
                     /gene="flgL"
                     /locus_tag="PLS34_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRP86775.1"
                     /translation="MRISTSQIYNAGMDSIQRQQAEVNRVQQQLASGKRILSPSDDPG
                     GAVQSLQFRDSMARLEQYARNGSLAQSRLNQEENVLGQAMDSLQRVRELAIQANNASQ
                     TPETRRSIAVEIRQQLEQMYQLANTRDANGEYIFAGFASLDRPFVATPAGAQYNGGAQ
                     ARQVAISDERRIALGDTGAAVFMDIPEGNGRFVVVEDAANTGTGAVQETSVSSPGAWD
                     GTERTLRFTAPDTWEALDAASNVVASGSYSSGGTITFEGLSVRLGGSPAAGDSFSLRA
                     SAGQDIFAIYSELADALEHPVAGAADAARQTGAIGRALSDLDQAAGQFSEVRSTVGAR
                     LAAVDSQAEQREIEWLELSRTLSEIEDLDYAEAISRFNLRMVGLQAAQQSYSMFARLS
                     LFDFLR"
     gene            4567..5370
                     /locus_tag="PLS34_04555"
     CDS             4567..5370
                     /locus_tag="PLS34_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRP86776.1"
                     /translation="MAQIINTNILSLNAQRNLTTSQGALQTSLQRLSSGLRINSAKDD
                     AAGLAISERFTTQIRGLNQAVRNANDGISLAQTAEGALSEVTNNLQRIRELAVQAANA
                     TNSDEDREALDAEVQTLIEEIGRVADSATFNGVQLLNATADLTFQVGANNGDTVTVST
                     VDVSDVITGNVEDVAGANALMDAVDTALETVNSTRADFGAIQNRFESIVRSLQTAAEN
                     LSASRSRIMDADFAAETAALTRAQILQQAGVAMLAQANAAPQSVLNLLR"
     gene            5483..6805
                     /gene="fliD"
                     /locus_tag="PLS34_04560"
     CDS             5483..6805
                     /gene="fliD"
                     /locus_tag="PLS34_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRP86777.1"
                     /translation="MAGISSLGVGSGLDLNSLVRQLVAAERAPADARLNRLESRVESQ
                     ISGLGQVKSSLAKLEEALARLTGVEDARSVRLSDETAFSATATAAADVGRYNIEVFEL
                     ARAQSLASLGFADPDAPLGAGDLTISLGAGDPVTVTIAEGANSLRDVRDAINGADAGV
                     LATLVRDGAEWRLLLSAEESGLANTVSVSVSGSLHANLASAAMTETVTPADARFSVAG
                     LELTSSSNVVDDVLPGVTLNLRRITEAPVELAVGRDAEATRAALDEFVTAYNAMIDLA
                     ASLSSFNTETGTRAALNGDSTLRAIRGALPAALGADTGAGWNAVELGLRSDRNGKLSV
                     DDEAFAARLAENPAQALASLRGFGEALAGNIGIYTDTGGVLATRTESLQSSLRSLELR
                     RDQLDRRMESVEARLVRQFTALDQMISQMQNTSSFLASRLATLPVPGA"
     gene            6858..7265
                     /gene="fliS"
                     /locus_tag="PLS34_04565"
     CDS             6858..7265
                     /gene="fliS"
                     /locus_tag="PLS34_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRP86778.1"
                     /translation="MNPHSMRSALKEYEQVGVRSQVEGAPPERLVQLMLEGALDRVSL
                     ARGDMENGQIAAKGERIGKAISLVEGLRATLDHERGGELAGNLDALYEYISRRLTEAN
                     LRNDVAILDEVSRLLREIKQAWDRIVGGPEFAP"
     gene            7262..7597
                     /locus_tag="PLS34_04570"
     CDS             7262..7597
                     /locus_tag="PLS34_04570"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HRP86779.1"
                     /translation="MMPAPVPRPEGAGPQAALAALLETAHALDAAAAADAWDEAMELE
                     ALCRALLEELLSGPQDLAQAEAIETVAVIYRRVMSLAENCRAAVAEELNQLRQGRRAS
                     SAYNAERGA"
     gene            7594..8151
                     /locus_tag="PLS34_04575"
     CDS             7594..8151
                     /locus_tag="PLS34_04575"
                     /inference="COORDINATES: protein motif:HMM:NF028133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP86780.1"
                     /translation="MNSSVLEGRLSWEGALPLDWRALDRLPGRDQLARTDERNIALLN
                     ALTLIEEGSTRQTEEPSPLIAQMERLEAKFDLVLTLLTEALLPESVMPGLVHMRLSSR
                     GLVFEPGDAVEPGSPIELSIHFSASLPRPVVLYGVVIEPLPGEEAQVSVEFRPHDPAV
                     KERLERLVFRRHRRSIAQQRPKTAQ"
     gene            8355..9818
                     /locus_tag="PLS34_04580"
     CDS             8355..9818
                     /locus_tag="PLS34_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRP86781.1"
                     /translation="MDAASLERLPPAMIVVADSEIADELSGALAFIGLRCEVVPPDSI
                     AEHADAGDRWSAVMLCDGVGGPALKMLDGWHDGPPVIAVGLGQPIPERLKSLHARLLG
                     RVERPLRHAQLVPLLGKLARHKPAAQRDVELFRSLVGKSEPVQNIRRMIQRVAPTDST
                     VLLLGETGTGKEVAARHIHYHSQRRDGPFVAVNCGAIPADLLESELFGHEKGAFTGAL
                     TARKGRFELAENGTLFLDEIGDMSLQMQVKLLRVLQERQFERLGSGRPMPANVRIVCA
                     THRNLESMVEEGTFRGDLYYRINVFPIEMPALRERREDLPLLAAELVARLEAEGRGAV
                     SFTQDALAAMARHDWPGNVRELANLVERLAILHPGAPVGAQDLPPHIRAHARTGDAGA
                     PPAGFMTPTVERAAPPAPAQAQAEVPAEAEEPDFGPGRGLKDYLNDIEEQLIRRALAE
                     SGGIVADAAKSLGMRRTTLVEKLRKFGIDRLGFATET"
     gene            9991..10977
                     /locus_tag="PLS34_04585"
                     /pseudo
     CDS             9991..10977
                     /locus_tag="PLS34_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            10994..11311
                     /gene="fliE"
                     /locus_tag="PLS34_04590"
     CDS             10994..11311
                     /gene="fliE"
                     /locus_tag="PLS34_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRP86782.1"
                     /translation="MSDMQIQQVIHQMRALAERSPVRPAAEAAEGAQGFANVLKQSLE
                     SVNRQQAESTRLQEAFQRGDPGINLAQVMVAAQKSSIGFEATVQVRNRLLQAYQDIMN
                     MQV"
BASE COUNT         1837 a         4075 c         4009 g         1590 t
ORIGIN      
        1 cgaactggcc gcgcccgtgg atgccacttc ggtgcgcgtg cgcgcgccgc tggacccggg
       61 ccagcgggtc ggcttcatgt cctggttgga gaacctgacc gtggcgccgg gcgatccgcc
      121 cgcgcgggtg atcgtcaatt cccgcaccgg cacgatcgtc atgggcggca acgtcaaggt
      181 gctggccgcc gcagtggccc atggcggcct gtcggtcacc atctcggaga gcttcgcggt
      241 gtcgcagccc gagcctttct cgcgcggcgg cgaaacggtg gtggtgccgc agagcgaggt
      301 ccaggtcacg gagggcgacg ggcgcatgtt ccccttcggc cccggcgcga cgctcgagga
      361 aatcgtgcgc gcggtcaacc aggttggcgc ggccccgggc gacctgatcg ccatcctgga
      421 agcgctgcac cagtccggtg ccctgcgcgc cgagctgatc atcatctgag gacggccgac
      481 gcatgagcct gcccgcggtc accgacacga cggctacggc gaacctcgcc gatctgcggc
      541 gtgcggcgcg tgccggcgac gcgagcgcgg cgcgcgaggc ggccaaacag ttcgaggcgc
      601 tgttcgtcaa catgatggtc aagcagatgc gcgacgccat gccgaccgac ggcggcctgt
      661 tcggcggcga gggcatgaag ctctacgaga gcatgcacga ccagcagctt tcgctggcca
      721 tggcccggcg cggcggcatc ggcctggcgg atgccatcga gcggcagctg ttgccgccgg
      781 cggccccggg cgcgtcaggc gaagaactgc ggccattgaa ttttccggcg catcgcctgc
      841 cggcgcgcgt cacgccgctg gtgccgctcg acccggcgac ggcggctgca ccgagccgac
      901 cgccgccaag ccccgaggaa ttcgtgcagc gcctgttgcc gcacgcccgg cgcgcagccg
      961 cccggctggg cgtcgcgccc gaggtgctga tcgcccagtc ggcgctggag accggctggg
     1021 gccgccacgt catgcgcgat gccgcggggc gctcgagcca caacctgttc ggcatcaagg
     1081 cctccggcgg ctggtccgga gccagcgtgc gggtgtcgac gctggagtac cgcgacgggg
     1141 tggcgcgccg cgagcaggcg gccttccgcg actatcccga cgaggcggcg agcttcgcag
     1201 actatgtccg cctggttggc ggccacccga gataccgcca ggccgtcgcc aacgccgaca
     1261 gcccggaagg ctacctgcgt ggcctgcagg aggcgggcta cgccaccgac ccggcgtacg
     1321 ccgaaaagat tctttccatc ctggagcgcg gcctgccggg cctgcccggc ggcgatgcgg
     1381 ccggcgcgca gttcaagttc cccggccagc ggccgccaaa caaccaaacg ggcgacccgg
     1441 aagtcgtccg cacccggccg tccccggtcg gtgaggagat cagctgatgt ccggagtatt
     1501 cggaatcggc acctcggcgc tgctggccta ccagcgggcg ctgtcggtga ccggccagaa
     1561 catcgccaac gtgggcaacg agaactacag ccgccagcgc atcgacctgg cggtgcgcaa
     1621 tccgcagttc tccggtgccg gctggtacgg acagggcgtc gatgtcgtcg gcgtgcagcg
     1681 gatcgccgac cagttcgtgc agcgccaggt gctcgacaac accgccggct cggcgcgcca
     1741 gcaggtctac taccagtacg cccagcgcgc cgacaacctg ctggcggacg ccgaggccgg
     1801 cctggccccg gtgctgcagc agttctttgc cgccgtgcag gacgtcgcca acgatccgac
     1861 ctcgacggcc gcgcgccagg tgctgctgag ccagggcgag gcgctcgccg accgcttcgc
     1921 cttcctgcag cgacggctgg acgaccagcg ccaggtggtg gaagggcaga tcggcgcggc
     1981 ggtcgacgag atcaacggcc tcgccgcagg cattgcgtcc ctgaaccaga agattgccga
     2041 gggcatcggc cagtccggcg gcgccatgcc gaacgacctg ctcgaccagc gcgatgccat
     2101 gatcgagcgg ctgtccgggc tggtcgcggt gcagacgctg ccgcagggcg acggcaccct
     2161 gaacgtcatg atcggcaacg gccagaatct cgtcatgggc agcacggcca ccgtgctgac
     2221 cgcacagccg ctcggcgccg atccctcgcg caccgagatc gccgtggtga accccggctc
     2281 gacggtgggc atcaccgaca tcatctcggg cggcgaactc ggtgcgctgc tcgatgtgcg
     2341 ccgcgagctc ctcgacccgg tccagaacgc gctcggccgc acggccgtgg gtctcgcgct
     2401 gtccttcaac gaactgcacc gccagggcgt ggacctggac ggcgtggcgg gcggcgattt
     2461 cttccagctg ccgcagggcg aagtcctcgt cggccggggc aacaccgcca ccgggcagcc
     2521 cgcgctgact ttcaccgacg ccgcggcgct gacggcgggc gactaccgcg tcagcttcga
     2581 cggcgttgaa tggcgcctga cacggaccgg ttccgcgacg ccgctggcga ctgcggcgcc
     2641 gggcgggacc ctggcggccg acggcttcga gcttgatttg tccggcattg ccggcgcgac
     2701 cgcgggcgac tcatttgacg ttcgcccgac gcgcacggcc gccgccggcc tgggtgtcgc
     2761 gatcactgac ccgcgtgcct tcgccgccgc cttgccgccc gcggcgccgg gcgaaagcgt
     2821 agtcggcgac aaccgcaacg cgctggcgct cgccggcctg gcctcggaac gcgtgctggc
     2881 caacggcacc accagcatcg ccgcgtccta caacgaactg gttgccaatg tcggggtgaa
     2941 gacgcgtcag gcgcagctgt ccgccgaggc gcagggccgc atgctggaag cctcgcgcgc
     3001 ccagcgcgaa tcgatttccg gggtcaacct cgacgaggag gcgtccaacc tgctgcgcta
     3061 ccagcaggcc tacgccgccg ccgcccaggt catcgccacc gccggcacga tgttcgacac
     3121 cctgctgatg gcggtccgga ggtaggccga tgcgcatctc gacttcgcag atctacaacg
     3181 ccggcatgga ttcgatccag cgccagcagg ccgaggtgaa ccgcgtgcag cagcagctcg
     3241 ccagcggcaa gcgcatcctg tcgccgtccg acgacccggg cggtgcggtg cagtcgctgc
     3301 agttccgcga ttccatggcc aggctggagc agtatgcgcg caacggcagc cttgcccagt
     3361 cgcgcctcaa ccaggaagag aacgtgctcg gccaggcgat ggacagcctg cagcgggtgc
     3421 gtgagctcgc catccaggcc aacaacgcct cgcagacgcc ggaaacccgg cgctcgatcg
     3481 cggtggagat ccgccagcag ctggagcaga tgtaccagct ggccaatacc cgtgacgcca
     3541 acggcgagta cattttcgcc ggcttcgcct cgctcgaccg gccgttcgtc gccacgcccg
     3601 ccggggcgca gtacaacggc ggcgcgcagg cgcgccaggt ggccatcagc gacgagcgcc
     3661 gcattgcgct gggcgatacc ggcgccgccg tgttcatgga catcccggaa ggcaacggtc
     3721 gcttcgtggt cgtcgaggac gccgccaata ccggcaccgg cgccgtgcag gaaaccagcg
     3781 tcagctcgcc gggggcctgg gacggcaccg agcgcacctt gcgcttcacc gcccccgata
     3841 cctgggaggc gctggacgcg gccagcaacg tggtggcgag cggcagctac tccagcggcg
     3901 gcaccatcac cttcgaaggg ctctccgtgc gcctcggcgg cagcccggcc gccggcgaca
     3961 gtttctcgct gcgcgcctcc gccggccagg acatcttcgc gatctactcg gagctggcgg
     4021 acgcgctcga gcacccggtc gcgggggctg cggatgccgc gcgccagacc ggcgccatcg
     4081 gccgcgccct gtcggacctc gaccaggccg ccgggcagtt ctccgaggtt cgctccaccg
     4141 tcggtgcgcg cctcgccgcc gtcgattcac aggccgagca acgcgagatc gagtggctcg
     4201 aactgagccg cacgctgtcg gaaatcgagg acctcgatta cgccgaggcc atctcgcgct
     4261 tcaacctgcg catggtgggc ctgcaggccg cgcagcagtc gtattcgatg ttcgcgcgcc
     4321 tctcgctgtt cgatttcctg cgctgagccc ggttccgtac agcacgccgg aatagtgtga
     4381 acggcgtcac gcaaaccccg gtgatgcaag tgaaaaaagc gcgcagcagc gcacgaaaaa
     4441 ccctcaagta ctgggtcgca acgccgataa gaagcctgac gcggcgcagt cacgccgcct
     4501 ggaagccccg ccccgggtgg tggctgactg aagcttgaga gtcaacaatt cttaggagta
     4561 ctagacgtgg cacagatcat caataccaac atcctgtcgc tgaacgcgca gcgcaacctg
     4621 acgacttcgc agggcgccct gcagacgtcg ctgcagcgct tgtcctcggg tctgcgcatc
     4681 aacagcgcca aggacgacgc ggcgggcctc gcgatttccg agcgtttcac gacccagatc
     4741 cgcgggctca accaggccgt ccgcaacgcc aacgacggca tctccctggc gcagaccgcc
     4801 gagggcgcgc tgtccgaggt gaccaacaac ctgcagcgca tccgcgagct ggccgtgcag
     4861 gctgccaacg ccacgaactc ggacgaggat cgcgaggcgc tggatgccga ggtgcagacc
     4921 ctgatcgaag agatcggccg ggtggccgac tcggccacct tcaacggtgt gcagttgctc
     4981 aacgccacag cggatctcac cttccaggtc ggcgcaaaca atggcgacac cgtgacggtc
     5041 agcaccgtcg acgtgagcga cgtgattacc ggcaacgtcg aggatgtcgc cggcgccaac
     5101 gcgctgatgg acgcggtcga cacggccctc gagaccgtga acagcacgcg cgccgacttc
     5161 ggcgccatcc agaaccgctt cgagtccatc gtgcgcagcc tgcagaccgc ggccgagaac
     5221 ctgtcggcct cgcgctcgcg catcatggac gcggacttcg ctgccgagac ggccgcgctg
     5281 acccgcgccc agatcctgca gcaggccggc gtggccatgc tggcgcaggc caacgcggcg
     5341 ccgcagagcg tcctgaacct gctccgctag gtccaggccg caagcccgga tcccgggatg
     5401 gtaaggcggc cggtgcgtgc atcagcccgg ccggccgccg cccacccggg accccggatg
     5461 aagtttccag gagttcatcg gcatggcagg catttcctcc ctcggcgtcg gctcaggact
     5521 ggacctcaac tccctggtcc ggcagctcgt cgccgccgag cgggcgccgg ccgacgctcg
     5581 cctcaaccgc ctcgagtcga gggtcgagtc ccagatctcc ggcctcggcc aggtcaagag
     5641 cagcctggcc aagctcgaag aggcgttggc caggctcacg ggcgtggaag acgcccgcag
     5701 cgtccggctg tccgacgaga cggccttctc ggccaccgcc acggcggccg ccgacgtggg
     5761 ccgctacaac atcgaggttt tcgagctggc ccgcgcccag tcgctggcca gcctgggctt
     5821 cgccgatcca gacgcccctc tcggcgccgg cgacctgacg atttctctcg gtgccggcga
     5881 tccggttacc gtgaccatcg ccgagggcgc caacagcctg cgcgacgtgc gcgacgccat
     5941 caacggggcg gacgccggcg tgctggcgac cctggtgcgc gacggcgccg agtggcgatt
     6001 gctgctcagc gccgaggaaa gcggcctggc gaacacggtc agcgtatcgg tctccgggag
     6061 cctgcacgcc aacctcgcct cggccgccat gaccgagacg gtcacgccgg cggacgcgcg
     6121 cttcagcgtg gccggcctgg aactgaccag ttcctcgaac gtggtcgacg acgtgttgcc
     6181 cggcgtcacg ctgaacctgc ggcgcattac cgaggcgccg gtcgagctcg ccgtgggccg
     6241 ggacgcggaa gcgacccgcg cggcgctgga cgagttcgtc accgcctaca acgcgatgat
     6301 cgacctggcc gccagcctgt cgagcttcaa taccgagacc ggcacgcggg cggccctgaa
     6361 cggcgactcc acgctccggg ccatccgtgg cgcgttgcct gcggcgctcg gcgcggatac
     6421 cggcgcgggc tggaacgccg tcgagctcgg gctgcgctcg gaccgcaacg gcaagctcag
     6481 cgtcgacgac gaggccttcg ccgcccgcct ggcggagaat cctgcccagg ccctggcctc
     6541 gctgcgcggc ttcggcgagg cgctggccgg caacatcggc atctacaccg acacgggcgg
     6601 ggtgctggcc acgcgcacgg agagcctgca gtcttcgctg cgctcactgg agctgcgacg
     6661 tgaccagctc gaccgccgca tggagagcgt cgaggcacgg ctggtcaggc aattcacggc
     6721 tctcgaccag atgatctcgc agatgcagaa caccagcagt ttccttgcgt cccgcctggc
     6781 caccctgccg gtgcccgggg cctgaggccg tgctcgccag accagcaggc ccatcgagag
     6841 gccgggaatc gaacgacatg aatccacaca gcatgcgcag cgcactgaaa gaatacgagc
     6901 aggtcggcgt ccgctcgcag gtcgagggcg cgccacccga gcgcctcgtg cagctgatgc
     6961 tggaaggggc ccttgatcgc gtcagcctgg cgcgcggcga catggaaaac ggccagattg
     7021 ccgccaaggg cgagcgcatc ggcaaggcca tcagcctggt ggagggcttg cgcgccacgc
     7081 tcgatcatga gcgcggcggc gagctggccg gcaacctcga tgcgctgtac gagtacatct
     7141 cgcgtcgcct gaccgaggcc aacctgcgca acgacgtggc gatcctcgat gaggtatcgc
     7201 gcctgctgcg cgagatcaag caggcctggg accgcatcgt cggcggcccg gagttcgcgc
     7261 catgatgccg gcacccgtgc cgcggccgga gggcgccggg ccgcaggccg ccctggcggc
     7321 cctgctggag accgcccatg ccctggacgc cgcggccgcc gcggacgcct gggacgaggc
     7381 catggagctc gaagccctgt gccgcgcact gctcgaggag ctgctgagcg ggccgcagga
     7441 cctggcccag gcggaagcga tcgagaccgt ggccgtcatc taccgccgcg tcatgagcct
     7501 cgccgagaac tgccgtgcgg cggtggccga ggaactcaac cagctgcgcc agggacgccg
     7561 ggccagcagc gcctacaacg cggagcgcgg tgcttgaatt ccagcgtcct cgaaggccgg
     7621 ctcagctggg agggcgccct gccgctcgac tggcgggcgc tcgatcgact gcctggccgg
     7681 gaccagctgg cacgcacgga cgaacgcaac atcgcgctgc tgaacgcgct gacactgatc
     7741 gaggaaggct cgacccgcca gaccgaagag ccgtcgccct tgattgcgca aatggagcgg
     7801 ctcgaggcca agttcgacct ggtgctgacg ctgctgaccg aggcgctgct gccggaatcg
     7861 gtgatgccgg ggctggtaca catgcgcctg tcgtcccgcg ggctggtgtt cgagcccggc
     7921 gatgcagtcg agcccggctc gccgatcgag ctgagcattc acttcagcgc cagcctgccg
     7981 cggccggtgg tgctgtacgg cgtggtgatc gagcccttgc caggcgagga agcgcaggtc
     8041 tcggtcgagt tccgcccgca cgatccggcc gtcaaggaac gcctggagcg gctggtgttc
     8101 cgccgccatc gccgcagtat cgcccagcag cggcccaaga ccgcccaata gcctcccgta
     8161 cgcccccccg tcagaaatgt gacgccaatc gtttaagctt ggctcacgtc cgttaacata
     8221 agcaccgcct tgcgcctgtc ggggaccggg agtccggtct cggggcggcg cacacaagaa
     8281 gacggaacgg atagcacagg gttgaccagg cgcaagcctg ccggggacac atggagccga
     8341 gtcgctcagg acaagtggac gctgcgtcgc tggagcgact gccgccggcg atgatcgtgg
     8401 tggcggacag cgaaatcgcg gacgagttgt ccggcgcgct ggcctttatc ggcctgcgtt
     8461 gcgaggtcgt cccgccggac tccatcgcgg agcatgccga cgccggcgac cgctggtccg
     8521 ccgtgatgct gtgcgacggc gtgggcggcc ccgccctcaa gatgctcgac ggctggcacg
     8581 acggcccgcc ggtgatcgcc gtcggcctcg gccagccgat cccggagcgc ctgaagagcc
     8641 tgcacgcgcg gctgctgggc cgcgtcgagc gtcccttgcg acatgcgcag ctggtgccgc
     8701 tgctcggcaa gctggcgcga cacaagcccg ccgcccagcg cgacgtcgag ctgttccgct
     8761 cgctggtcgg caagagcgag ccggtgcaga acatccgccg catgatccag cgcgtggcgc
     8821 cgaccgactc gaccgtgctg ctgctcggcg agaccggcac cggcaaggaa gtcgcggcac
     8881 ggcacatcca ctatcactcg cagcggcgcg acgggccgtt cgtcgcggtg aactgcggcg
     8941 ccattccggc cgacctgctg gagagcgagc tgttcggcca cgagaaagga gccttcaccg
     9001 gcgcgctgac ggcccgcaag gggcgcttcg agctggccga gaacggcacg ctgttcctcg
     9061 acgagatcgg cgacatgtcg ctgcagatgc aggtgaagct gctgcgcgtg ctccaggagc
     9121 gccagttcga gcgcctcggc agcggccggc cgatgcccgc caacgtgcgc atcgtctgcg
     9181 cgacgcaccg caacctcgag tcgatggtcg aggagggcac cttccggggc gatctctact
     9241 accggatcaa cgtcttcccc atcgagatgc ctgccctgcg cgagcggcgc gaggacctgc
     9301 cgctgctggc ggcggagctg gtggcccgcc tcgaggccga gggccggggc gcggtgagct
     9361 tcacccagga tgcgctggcg gcgatggcac ggcacgactg gccgggcaac gtgcgcgagc
     9421 tcgccaacct ggtggagcga ctggcgattc tgcaccccgg cgcgccggtc ggcgcgcagg
     9481 acctgccgcc ccacattcgc gcgcacgccc gaacgggcga tgcgggcgcg ccaccggcag
     9541 gtttcatgac gcccacggtg gagcgtgctg cgccgccggc gcccgcccag gcccaggccg
     9601 aggtgccggc cgaagcggag gagccggact tcgggccggg ccgcggcctc aaggactacc
     9661 tcaacgacat cgaggaacag ctgatccgcc gggcgctcgc cgaaagcggc ggcatcgtgg
     9721 cggacgccgc caagtccctc gggatgcgcc gcaccaccct ggtcgagaag ctccgcaagt
     9781 tcggcatcga tcgcctcgga ttcgcgacgg aaacttgacg gccgaccggc cgtccgccgg
     9841 ccggaccccc taagcaattg tttttcatgg cctcgccagg ttccggcatg gaacttgctg
     9901 taagtccgtg cggcggccgt ggggccgcga cggaccatgc cgggaggcga acgaatggag
     9961 gcagcactgc agctggacgg gcgtgacgcg gacgggctcg ggcccatcgc cgcggatccc
    10021 gtgagccgcg agttgctggc gctggccgcc cgggtagcgc gctcggacgc ggccgtgatg
    10081 ctgaccggcg agtcgggcgt cggcaaggag gtctacgcgc gcttcctgca ccgggcgtcc
    10141 gcccggcatg ccgggccgtt cgtggcgctg aattgcgcgg cgatcccgga gaatatgctg
    10201 gaggccatgc tgttcggcta tgagcgcggc gctttcaccg gcgccacgga gtcgcggccg
    10261 ggcaagttcg agcaggccga gggcggcacg ctgctgctcg acgaaatcac cgagatggag
    10321 atcggcctgc aggccaagct gctgcgggtg ctgcaggagc gggaggtcga gcgcatcggc
    10381 gggcgccgcg tcatccgttt cgacgtccgc atcgtctcca cctccaatcg cgacctgcgc
    10441 gcggcggtgg ccgagggccg cctgcgcgaa gacctgttct accggctgaa tgtgtttccc
    10501 ctgcacgtcc cggcgctgcg cgagcggccc ggtgacctgc tgccgctggc cagtcacctg
    10561 ctggagcgcc atgcccgggg ccgcgccctg cggctgaccc actgcgccca ggtgcgcctg
    10621 ctggcgcatt cgtggccggg caacgtgcgg gaactcgaca acgtcatgca acgcgcgctg
    10681 gtgctgggca acggccccac ggtggacgcg gcggccctgc acatcgagga caccagcccg
    10741 accatcccgg gcccggccac cgccgccgcg tcgatcgccg gcaactccgg tgcgagagat
    10801 gcagagagcg cgggcctcgg cgaggaagtc cggaaccacg aagagcaact gatcctgaac
    10861 gccctgcgcg tcgagcaggg cagccgcaag gacgcggccg cgcgactcgg catcagcccg
    10921 cggacgctgc gctacaagct ggcgcggatg cgcgaactcg gcatggcgat cccctaggcc
    10981 ccggagaagt tcaatgagcg atatgcagat ccagcaggtc atccaccaga tgcgcgcgct
    11041 cgccgagcgc agccccgtgc ggcccgcggc ggaggccgcc gagggcgcac agggcttcgc
    11101 caatgtgctg aagcaatcgc tggagagcgt caaccgtcag caggcggagt cgacccggct
    11161 gcaggaagcg tttcagcgcg gcgacccggg catcaacctc gcccaggtga tggttgccgc
    11221 acagaagtcc agcatcggct tcgaagcgac ggtgcaggtg cgcaaccgcc tgctgcaggc
    11281 ctaccaggac atcatgaaca tgcaggtctg aggacgacct ggtccccggc cttaccgtaa
    11341 tcaacgagcg acacaacgat ggagtttgac agggcatgag caaggcaatc acccgatccg
    11401 caggcgcccc gccggggaat cccctcgacg gcttcatgcg cctgccggca cagcgtcagg
    11461 tcggcctgct gctgagtctc gcgctggtgg tggcgctgct gggcgccgcc t
//
LOCUS       DAOMPG010000056        11485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2428_length_11485_cov_4.374366, whole genome shotgun sequence.
ACCESSION   DAOMPG010000056 DAOMPG010000000
VERSION     DAOMPG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_11485_cov_4.374366"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLS34_04595"
     CDS             complement(<1..655)
                     /locus_tag="PLS34_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP86783.1"
                     /translation="MNLFQEAQRYIPGGVNSPVRAFRSVGGDPVFIRKARGAYFWGED
                     GERYTDYVGSWGPMILGHAHPDVIEAVRTAALDGLSFGAPTAVETELAKRVCQIMPSI
                     ELVRFVSSGTEATMSAIRLARGFTGRDKIVKFEGCYHGHSDSLLVKAGSGALTLGVPT
                     SPGVPASLAQHTITLTYNDLDEVERVFAELGDEIACIIVEPIAGNMNMVLPAPGFLAG
                     "
     gene            complement(711..1361)
                     /gene="thiE"
                     /locus_tag="PLS34_04600"
     CDS             complement(711..1361)
                     /gene="thiE"
                     /locus_tag="PLS34_04600"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRP86784.1"
                     /translation="MRKPALPPGLYALTDPDLLPEAVLAERVAAAVRGGARIVQYRDK
                     GADADARQRRGRAVLEACRAHGALCIINDDAELATTVGADGVHLGRDDGDVAAARAVV
                     GPDRLIGVSCYDQLELADAAVAAGADYVAFGSVWPSGTKPDAVRAPLALLSRAARRLP
                     VPVVAIGGITRDNAAATIAAGAHCIAVIRDLFAGPDPCAAAQVLSAACERGRSGLE"
     gene            complement(1366..2151)
                     /locus_tag="PLS34_04605"
     CDS             complement(1366..2151)
                     /locus_tag="PLS34_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRP86785.1"
                     /translation="MSVIPPTVLVVAGNDPSGGAGIAADLQAITAAGAHPAPVITALT
                     VQDTVNASAVEPASPELVAAQMEAVLADIPVAAVKIGLLATAAIAEAVAAVLARHPGL
                     PVVLDPVLVAAGGARLAEDALVGVVLERLCPLTTLLTPNAHEIRRLAPDLDDTAARAA
                     ALRRAGCSFVLAKGGDEADAGGNEVLNTLYGPGEPRLFRWPRLEGSFHGSGCTLASAC
                     AARIACGDPVEEAVAHGQALTQRWLAHAFRPGRGQLVPLRRPG"
     gene            2223..2387
                     /locus_tag="PLS34_04610"
     CDS             2223..2387
                     /locus_tag="PLS34_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRP86786.1"
                     /translation="MKAWMCVICGYVYEEAEGDPDGGIPPGTPWDDVPLSWRCPDCGA
                     GKEDFEMIEI"
     gene            complement(2405..3277)
                     /locus_tag="PLS34_04615"
     CDS             complement(2405..3277)
                     /locus_tag="PLS34_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRP86787.1"
                     /translation="MKLRLLALLALGLQAGPLAAVPLWTVETEHGSGQVILLGSVHLL
                     RAEHLPLPDVVHEAYEGADRLVMELDPAELTPGASQAALQRIGITSPGGSAAELLDAE
                     QWRLAEDLAAEAGVDLQAVAGLEPWFGAIVLYTSALIAAGYDPALGVDQQIAGWAARD
                     AKPAAGLETLDEQLLLFKRLGTDTQREMLLKTLEELVTMEADTDALVGQWRAADLHAL
                     SRRLDTDFRNYEELRESIVEERNRNWLPAVAELLEQGGTSLVVVGTLHLVGPEGLPAL
                     LAERGFRVMPVAAR"
     gene            complement(3274..3861)
                     /locus_tag="PLS34_04620"
     CDS             complement(3274..3861)
                     /locus_tag="PLS34_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRP86788.1"
                     /translation="MNPSGDLRAALVRSYRWLRQHGLNDSHSGNASARDGDIAWVTPS
                     GCGGDTLEPDDLLPCRPGEAPPSGASLDAPLHLAVYAACPEARAILHSHGPHSIAMTL
                     AGADFVPADFEGRYYFPVVPVLDIPPEDYTRRSPAEVAATLARHRVCVVRGHGVYAAA
                     ASLDLAYKWTCSLESSARIAWLARAAGIPANGGSA"
     gene            complement(3858..4640)
                     /locus_tag="PLS34_04625"
     CDS             complement(3858..4640)
                     /locus_tag="PLS34_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP86789.1"
                     /translation="MGGDRPLRLLQLTDSHLRAAADGALKGWRTRESLELALRAALAG
                     EAAPDAILATGDLSQDGSAASYGHVRDLLGGLGVPVFCIPGNHDDPQTMAAELSQPPF
                     HYCGDHGLGPWRLVMLATWDGDRGGGRLPEQELLRLAATLEGTAEPHVLVVLHHHPVP
                     MNSWLDKVALDNADEFLAVTDSCPRVRGVLWGHVHQAHDSERRGVRMLGTPSTCFQFV
                     PRVQQSDVDPDAGPGWRWLELGSDGGITTRVGAAPKPAGFGR"
     gene            4715..5428
                     /locus_tag="PLS34_04630"
     CDS             4715..5428
                     /locus_tag="PLS34_04630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP86790.1"
                     /translation="MSNRSIGLEPRLYEYLLSASLREHPVLARLRAETASHPRVNMQI
                     APEQGQFMALLVRMLGAVRCIEVGVFTGYSSLAVALALPPTGRIIACDVSEEYTQIAR
                     RYWQEAGVAERIDLRIAPALETLDRLLAAGEAQQYDFAFIDADKGAYADYYERLLELL
                     RPGGLIAVDNTLWDGDVAVPVNQAPDTVAIRAFNEKLRDDPRIDLSLVPIGDGLTLAR
                     KKNRDKNRGQRRVSKETRL"
     gene            complement(5457..6875)
                     /gene="ahcY"
                     /locus_tag="PLS34_04635"
     CDS             complement(5457..6875)
                     /gene="ahcY"
                     /locus_tag="PLS34_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRP86791.1"
                     /translation="MSSQTAQALKPDYLVADIGLADWGRKEIAIAETEMPGLMALRAK
                     HGKDQPLKGARIAGSLHMTIQTAVLIETLVELGADVRWASCNIYSTQDHAAAAIAKAG
                     VPVFAFKGETLDEYWEFTHRILEWGDGGLPNMILDDGGDATLAVTLGARAEQDPSLLE
                     NPSSEEEVALFKALKRRIEARPGWYAKAQQSIRGVTEETTTGVHRLYQMEKEGRLPFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIALVAGYGDVGKGCAQSLRG
                     LGATVWVTEIDPICALQASMEGYRVVTMDEAADKADIFVTATGNYHVITREHMARMKN
                     QAIVCNIGHFDNEIDVAGLKQYQWDNIKPQVDHIIFPDGKRIILLAEGRLVNLGCAHG
                     HPSFVMSNSFTNQVLAQIELHNNKGQYDHRVYVLPKHLDEEVARLHLERIGARLTTLR
                     PDQAKYIGVDVAGPYKPDHYRY"
     gene            complement(6926..8080)
                     /gene="metK"
                     /locus_tag="PLS34_04640"
     CDS             complement(6926..8080)
                     /gene="metK"
                     /locus_tag="PLS34_04640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRP86792.1"
                     /translation="MANDFLFTSESVSEGHPDKIADQVSDAVLDAILKEDPRARVAVE
                     TMVKTGMAIIAGEVTTTAWVDLEDLTRRTILDIGYNSSQLGFDGATCAVLNAIGKQSP
                     DIAQGVDRSDPRSQGAGDQGLMFGYATNETESLMPAPIHFAHKLVERQAIERKNGTLP
                     WLRPDAKSQITFRYEDNRPVGIDAVVLSTQHSPEIGQKELQEAVMEVIIKPVIPEEWL
                     ASCKRFHINPTGAFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSA
                     AYAARWVAKNIVAAGLADRCEIQISYAIGVAEPTSITVETFGTGKVSNQRLTQLVREH
                     FDLTPYGIIQGLDLIRPIYQATAAYGHFGRDNPAFTWEHTDKAEELRNEA"
     gene            8191..8745
                     /locus_tag="PLS34_04645"
     CDS             8191..8745
                     /locus_tag="PLS34_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP86793.1"
                     /translation="MARTDTPAATLGARAPDFTLCDSEGRAWSLADCRGPQGTVVMFI
                     CNHCPYVQAALERIVRDCRELLELGVASVAIMPNDIEAYPQDGPEAMARLAREMDLPF
                     PYLLDSSQQVARAYGAVCTPDFFGYDADLRLCYRGRIDGATPGRPAAAERRELFEAMR
                     EVAATGRGPDQQLPSVGCSIKWRD"
     gene            8865..10868
                     /gene="tkt"
                     /locus_tag="PLS34_04650"
     CDS             8865..10868
                     /gene="tkt"
                     /locus_tag="PLS34_04650"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRP86794.1"
                     /translation="MPSRRDLANAIRALSMDAVQQANSGHPGMPMGMADIAEVLWNDF
                     HRHNPANPRWANRDRFVVSNGHGSMLLYSLLHLSGYPLAMDEIRNFRQFGCRTAGHPE
                     YEPGLGIETTTGPLGQGLANAVGMAIAERALAARYNRPGFEVIDHHTWVFAGDGCLME
                     GVSHEACSLAGTLGLGKLIVFYDDNGISIDGEVEGWFTDDTPARFGAYGWHVVPGVDG
                     HDPAAVRAAIEAARAETGRPTLICCKTVIGWGAPNKQGTEATHGAALGADEVARTREN
                     IGWPHPAFEIPQEIRAGWDARERGAAAEAQWDTLLAAYAKEYPAESAELVRRLAGELP
                     AGWSQAADEVIARLAESAADVATRKASELALNGFGRLLPELIGGSADLSGSNNTIFKG
                     SRRIGAGEFTGNYLHYGVREFGMTAIMNGMALHGGFKPYGGTFLVFSDYARNAVRMAA
                     LMRQPVTLVYTHDSIGLGEDGPTHQPIEHVASLRLIPNLRVWRPCDLAETAVAWRDAL
                     ESTATPTALALSRQNLPAQPRNPEQLAAIRRGGYVLHEPASAPAALIIATGSEVDLAM
                     QAAALLLAEGVAVRVVSMPCVEIFLEQDAAWRDAVLPPHITARLAVEAGVPDGWCPFV
                     GGAGAVVGIRRFGESAPAGKLFEHFGFTPQAVAARLRALIKGS"
     gene            10880..>11485
                     /locus_tag="PLS34_04655"
     CDS             10880..>11485
                     /locus_tag="PLS34_04655"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRP86795.1"
                     /translation="MAIKVGINGYGRIGRNILRALYESGRTGEISIVAINDLGDANTN
                     AHLTRYDTAHGKFSGKVEVDGDSLVVNGDRIRVLAERNPANLPWKELGVEVVLECTGL
                     FTSKEKASAHLQAGAKKVIISAPGGKDVDATIVYGVNHDTLKSKHEVISNASCTTNCL
                     APLVQPLHQAIGVVNGLMTTIHAYTNDQVLTDVYHSDLRRAR"
BASE COUNT         1740 a         3935 c         4020 g         1790 t
ORIGIN      
        1 ggccggccag gaaaccgggg gccggcagca ccatgttcat attgccggcg atcggctcga
       61 cgatgatgca ggcgatctcg tcaccaagct ctgcgaatac ccgctcgacc tcgtcgaggt
      121 cgttataggt gagcgtgatg gtgtgctgcg cgagcgaggc cggcacgccg ggtgaagtcg
      181 gcacgcccag cgtcagcgcg cccgagccgg ccttcaccag cagggagtcg gaatggccgt
      241 gatagcagcc ctcgaacttg acgatcttgt cgcggccggt gaagccgcgg gccaggcgga
      301 tggcgctcat ggtggcctcg gtgccggagc tgacgaagcg caccaattcg atggacggca
      361 tgatctggca gacgcgcttg gcgagttcgg tctcgaccgc ggtcggggcg ccgaaggaga
      421 gtccgtcgag cgccgccgtg cggaccgcct cgatgacgtc cgggtgggcg tggccgagga
      481 tcatcgggcc ccaggagcca acgtagtccg tgtagcgctc gccgtcctcg ccccagaagt
      541 aggccccgcg cgccttgcgg atgaacacgg gatcgccgcc gacggagcgg aaggcgcgga
      601 ccggcgagtt gacgccgccg gggatgtaac gctgggcttc ctggaacagg ttcacgatgc
      661 gcgagcctcc tgggactgac cgggcggcag tgcgccgccc gcaaaaaccg tcattctagc
      721 ccgctgcggc cgcgctcgca ggctgcggaa agtacctgcg ccgccgcgca aggatcgggt
      781 ccggcgaaca ggtcacggat caccgcgatg cagtgcgcgc ccgcagcgat cgtggccgcg
      841 gcgttgtcac gcgtgatgcc gccgatggcc accaccggca ccggcaggcg ccgggccgcg
      901 cggctgagca gggcgagcgg cgcacgcacc gcgtccggtt tcgtgcccga gggccacacg
      961 ctgccgaagg cgacgtagtc ggcgcccgcc gccactgcgg catcggccag ctccagctgg
     1021 tcgtagcagg agacgccgat caatcggtcc gggccgacca cggcgcgggc cgccgccaca
     1081 tcgccgtcgt cgcggccgag gtgtacgccg tcggccccga ccgtcgtggc gagttcggcg
     1141 tcgtcgttga tgatgcacag cgcgccgtgc gcgcgacagg cctcgagcac cgcgcgcccg
     1201 cgccgctggc gggcgtcggc gtcggcgccc ttgtcgcgat actgcacgat gcgcgcgccg
     1261 ccgcgcaccg ccgccgccac ccgttcggcc agcaccgcct cgggcagcag gtcgggatcg
     1321 gtcagggcgt acagccccgg cggcagggcc ggcttgcgca cggtctcagc ccggcctgcg
     1381 cagcggcacg agctggccgc ggcccggccg gaaggcgtgc gccagccagc gctgggtcag
     1441 ggcctggccg tgggcgacgg cttcctccac ggggtcaccg caggcgatgc gcgccgcaca
     1501 ggccgacgcg agcgtgcaac ccgagccatg gaagctgccc tcgaggcggg gccagcgaaa
     1561 caatctcggc tcgcccggtc catacagcgt gttgagcacc tcgttgccgc ctgcgtccgc
     1621 ctcgtcgccc cccttggcca gcacgaaact gcagccggcg cggcgcagcg ccgcagcgcg
     1681 cgccgcggtg tcgtcgaggt cgggcgcaag ccggcggatc tcgtgggcgt tgggcgtcag
     1741 cagggtggtc agcggacaca gtcgctccag caccacgccg acgagcgcat cttcggcgag
     1801 gcgcgcgccg ccggcggcga ccagcaccgg atccagcacc accggcaggc cgggatggcg
     1861 ggccagcacg gctgcgacgg cctcggcgat cgctgcggtc gcgagcaggc cgatctttac
     1921 ggcggccacc ggtatgtccg ccagcacggc ctccatctgg gcggccacca gctcgggcga
     1981 ggccggttcg acggcgctgg cgttcacggt gtcctggacg gtcagcgccg tgatcaccgg
     2041 tgcaggatgg gcgccggcgg cggtgatcgc ctgcaggtcg gcggcgatgc ccgcgccgcc
     2101 ggacggatcg ttgccggcca cgaccagcac ggtgggtgga atgacggaca tatggcttgg
     2161 gtcgggggct actgcatgcg agaattcgcg gctgtttgga ttatcgccgc ggtgggccat
     2221 tgatgaaagc ttggatgtgc gtgatttgcg ggtacgtcta cgaggaagcc gagggcgacc
     2281 cggacggcgg catcccgccc ggcaccccgt gggacgacgt gccgctcagc tggcgatgcc
     2341 cggactgcgg cgccggcaag gaagatttcg agatgatcga gatctagcgg ccggtcgccg
     2401 cgtttcagcg cgcggcgacc ggcatgaccc ggaagccgcg ctctgcgagc agggccggca
     2461 ggccttccgg tccgaccagg tgcagggtcc cgaccaccac caggctggta ccgccctgct
     2521 cgagcagctc cgcgaccgcc ggcagccagt tgcggttgcg ctcctcgacg atactctcgc
     2581 gcaattcttc gtagttgcgg aagtcggtgt ccaggcggcg gctcaatgcg tggaggtcgg
     2641 cggcccgcca ttgtccgacc agcgcatccg tgtccgcctc catggtgacg agttcttcca
     2701 gcgtcttgag gagcatctcg cgctgggtgt ccgtgccgag acgcttgaac agcagcagct
     2761 gctcgtcgag ggtctccagg cccgccgccg gcttggcgtc gcgcgcggcc cagccggcga
     2821 tctgctggtc cacgccgagc gccgggtcat agcccgcggc gatcagcgcc gaggtgtaca
     2881 gcacgatcgc gccgaaccac ggctccagcc cggccacggc ctgcaggtcg acgccggcct
     2941 cggcagccag atcctcggcc agccgccatt gctcggcatc gagcagctcg gctgcggagc
     3001 cgccggggga ggtgatgccg atgcgctgca aggccgcctg ggaggcaccg ggcgtgagct
     3061 cggcggggtc cagctccatc accagccggt ccgctccctc gtaggcctcg tggacgacgt
     3121 ccggcaacgg caggtgctcg gcgcgcagca ggtgcaccga gcccagcagg atgacctgcc
     3181 cgctgccatg ctccgtctcg acggtccaca gcggcactgc cgccagcggc ccggcctgca
     3241 gccccagtgc cagcaatgca agcaggcgga gcttcatgcg ctgccaccgt tcgcggggat
     3301 gccggcggcg cgcgccagcc aggcgatgcg cgccgaggac tccagcgagc aagtccactt
     3361 gtaggccaga tcgagcgacg ctgcggcggc gtacacgccg tgtccccgca ccacgcaaac
     3421 gcgatgccgc gccagcgtcg ccgcgacctc ggccggcgag cggcgcgtgt agtcctcggg
     3481 cgggatgtcc agcaccggta ccaccgggaa gtaataacgc ccctcgaaat cggcgggcac
     3541 gaaatcggcg ccggccagcg tcatcgcgat gctgtgcggc ccgtggctgt gcaatatggc
     3601 ccgcgcctca gggcaggctg catagacggc gaggtgcagc ggcgcgtcca gcgatgcgcc
     3661 cgacggcggc gcttcgccgg gcctgcaggg caggaggtca tcgggctcga gcgtgtcgcc
     3721 gccgcacccg ctcggcgtga cccaggcgat gtcgccgtcg cgagccgatg cattgccgct
     3781 gtgggaatcg ttcaggccat gctggcgcag ccagcggtaa gagcgcacca gcgctgcgcg
     3841 gaggtcgccg gatgggttca ccggccgaac ccggccggct tgggcgccgc accgacccgc
     3901 gtggtgatgc cgccgtcgct gcccagctcc agccagcgcc agccgggacc ggcgtccggg
     3961 tcgacatccg attgctggac ccggggcacg aactgaaaac aagtcgaagg cgtgccgagc
     4021 atgcgtacgc cgcgacgctc gctgtcgtgg gcctggtgaa cgtgccccca cagtacgccg
     4081 cggacccgcg ggcagctgtc cgtcacggcg aggaactcgt ccgcgttgtc cagcgccacc
     4141 ttgtcgagcc aggagttcat cggcaccggg tgatgatgca ggaccaccag cacgtggggt
     4201 tcggcggtgc cctccagtgt tgcggcgagg cgcagtagct cctgctccgg cagccggccg
     4261 ccaccgcgat cgccgtccca ggtggccagc atcaccaggc gccaggggcc caggccgtga
     4321 tccccgcagt agtgaaaggg cggctgcgac agctccgcag ccatggtttg cgggtcgtca
     4381 tgattaccgg gaatgcagaa taccggcacg ccgaggccgc cgaggaggtc gcgcacatgg
     4441 ccgtaggatg ccgcactgcc gtcctgcgac aggtccccgg tcgccaggat ggcgtccggc
     4501 gcggcctcgc cagccagcgc ggcccgcagc gcgagttcca gcgactcccg ggtgcgccag
     4561 cccttgagcg cgccgtcggc tgcggcgcgc aagtggctgt cggtcagttg cagcaggcgc
     4621 agcggacgat ccccgcccat ctcccgtacc ccgttagaat tcagtgcagc gactagtcta
     4681 accaaagcgg catcgccaaa tcgggaactg cgtcatgtct aaccgaagca tcggtctcga
     4741 gccccgtctc tacgaatacc tgctctcggc ctccctgcgc gagcaccccg tgctggcccg
     4801 cctgcgcgcc gagacggcca gccacccgcg ggtcaacatg cagatcgcgc cggagcaggg
     4861 ccagttcatg gcgctgctgg tacgaatgct cggcgcggtc cgctgcatcg aggtcggcgt
     4921 gttcaccggt tacagcagcc tggccgtcgc cctggcgctg ccgccgaccg gccggatcat
     4981 cgcctgcgac gtcagcgagg agtatacgca gattgcacgg cgttactggc aggaggcggg
     5041 cgtcgcggag cgcatcgacc tgcgcatcgc gccggccctc gagaccctcg acaggctgct
     5101 ggcggccggt gaagcgcaac aatacgactt cgcattcatc gacgccgaca agggcgccta
     5161 tgccgactac tacgagcgct tgctcgagct gctgcggccg ggcgggctca tcgccgtgga
     5221 caacaccctg tgggacggcg acgtcgccgt ccccgtcaac caggcgcccg ataccgtggc
     5281 catccgtgcc ttcaacgaga agctccgtga cgatccgcgc atcgacctct cgctggtgcc
     5341 gatcggcgac ggcctgacgc tggcgcgcaa gaaaaaccgg gacaaaaacc ggggtcagag
     5401 acgggtttct aaggaaaccc gtctctgacc ccggtttttg ttgttgccgg tttcattcag
     5461 tagcggtagt ggtccggctt gtaggggccg gcgacgtcca cgccgatgta cttcgcctgg
     5521 tcggggcgca gggtcgtcag cctggcgccg atccgctcga ggtgcagccg cgccacctcc
     5581 tcgtcgagat gcttcggcag cacgtacacc ctgtggtcat actggccctt gttgttgtgc
     5641 agctcgatct gcgccagcac ctggttggtg aagctgttcg acatcacgaa gctcgggtgg
     5701 ccgtgcgcgc agcccaggtt caccagccgg ccctcggcca gcaggatgat gcgcttgccg
     5761 tcagggaaga tgatgtgatc gacctgcggc ttgatgttgt cccactggta ctgcttcagg
     5821 ccggccacgt cgatttcgtt gtcgaagtgg ccgatgttgc agacgatggc ctggttcttc
     5881 atccgggcca tgtgctcgcg ggtgatgacg tggtagttgc cggtcgcggt gacgaagatg
     5941 tccgccttgt cggcggcctc gtccatggtc acgacgcggt agccttccat gctggcctgc
     6001 agcgcacaga tcgggtcgat ctcggtgacc cacaccgtgg cgcccaggcc gcgcagggac
     6061 tgcgcgcagc ccttgcccac gtcgccgtag ccggccacca gcgcgatctt gccggcgatc
     6121 atgacgtccg tggcgcgctt gatgccgtcc accagcgatt cacggcagcc gtacaggttg
     6181 tcgaacttgc tcttggtgac cgagtcgttc acgttgatgg cggggaaggg cagtcggccc
     6241 tccttttcca tctggtagag ccggtgcacg cccgtggtgg tctcctcggt cacgccgcgg
     6301 atgctctgct gcgccttggc ataccagccc gggcgggcct cgatgcggcg cttgagcgcc
     6361 ttgaacagcg cgacctcttc ctcgctggag gggttctcca gcagcgaggg gtcctgctcg
     6421 gcgcgcgcgc cgagcgtcac ggccagcgtg gcgtcgccgc cgtcgtccag gatcatgttg
     6481 ggcagcccgc cgtcgcccca ctccaggatg cggtgcgtga actcccagta ctcgtccagc
     6541 gtctcgccct tgaaggcgaa caccggcacg ccggccttgg cgatcgcggc ggcggcgtga
     6601 tcctgggtgg agtagatgtt gcaggacgcc cagcgcacgt cggcgccgag ctcgaccagc
     6661 gtctcgatca gcaccgccgt ctggatggtc atgtgcaggc tgccggcgat ccgggcgccc
     6721 ttcagcggct ggtccttgcc gtgctttgca cgcagcgcca tcaggccggg catctcggtc
     6781 tcggcgatcg cgatttcctt gcgtccccag tcggccaggc cgatgtcggc caccaggtaa
     6841 tcgggcttga gggcttgtgc ggtctggctg ctcatgtctg ctccggaatc aagaatgggt
     6901 catgcaaggt gctttcgccg gaaactcacg cctcgtttcg cagttcctcg gccttgtccg
     6961 tgtgctccca ggtgaaggcc gggttgtcgc ggccgaaatg cccgtacgcg gcggtcgcct
     7021 ggtagatggg ccggatgagg tccagcccct ggatgatgcc gtacggcgtc aggtcgaagt
     7081 gctcgcgcac cagctgggtc aggcgctggt tggacacctt gccggtaccg aaggtctcga
     7141 ccgtgatcga ggtgggctcg gcgacgccga tggcgtagga aatctggatt tcgcagcggt
     7201 ccgcgaggcc tgccgcaacg atgttcttcg cgacccagcg cgccgcgtac gcggccgagc
     7261 ggtcgacctt ggaggggtcc ttgcccgaga aggcgccgcc gccgtgccgc gccatgccgc
     7321 cgtaggtgtc gacgatgatc ttgcgcccgg tcagcccgca gtcgcccagc ggcccgccga
     7381 tgacgaaggc gcccgtcgga ttgatgtgga agcgtttgca ggaggccagc cattcttccg
     7441 ggatcaccgg cttgatgatc acctccatga ccgcttcctg cagctctttc tggccgatct
     7501 ccggggagtg ctgggtggac agcacgacgg catcgatgcc gaccggccgg ttgtcctcgt
     7561 agcggaaggt gatctggctc ttggcgtccg ggcgcagcca cggcagcgtg ccgttcttgc
     7621 gctcgatggc ctggcgctcg accagcttgt gcgcgaagtg aatcggcgcc ggcatgagcg
     7681 actcggtctc gttggtggcg taaccgaaca tcaggccctg gtcgccggcg ccctgcgagc
     7741 gcgggtcgct gcggtccacg ccctgcgcga tgtccggcga ctgcttgccg atggcgttca
     7801 gcaccgcgca ggttgcgccg tcgaagccga gctgcgagct gttgtagccg atgtcgagaa
     7861 tggtgcggcg ggtgagatct tccaggtcca cccaggcggt ggtcgtgact tcgccggcga
     7921 tgatcgccat gccggtcttg accatggttt cgacggcgac gcgggctctg ggatcctcct
     7981 tgaggatcgc atcgagcacg gcgtcggaga cctggtccgc gatcttgtcc gggtggccct
     8041 cggagacgga ttccgaggtg aacaggaagt cattggccat gcgctgggtt ccgtaatggg
     8101 gctgaaaaag ggcgatagta taacggctgc ggcccttcga agggccacgc gcgtcacata
     8161 agccgccgcg ccgcggcaag gagcgagagc atggcaagga ccgacacccc ggccgcgacc
     8221 ctcggcgccc gtgcgcccga tttcacgctt tgcgactccg agggccgagc ctggagcctg
     8281 gccgattgcc gcggcccgca gggcaccgtg gtgatgttca tctgcaacca ctgtccctac
     8341 gtgcaggcgg cgctggaacg catcgtgcgg gactgccgcg agctgctcga gctcggcgtg
     8401 gccagcgtcg cgatcatgcc caacgatatc gaggcttacc cgcaggacgg cccggaggca
     8461 atggcgcgcc tggccaggga aatggacctg ccttttcctt acctgctgga ctcgagccag
     8521 caggtcgccc gcgcctacgg ggccgtgtgc acgcccgatt tcttcggcta cgacgccgac
     8581 ctcaggctgt gctaccgcgg ccggatcgac ggcgccacgc cgggccggcc ggcagccgct
     8641 gagcgccgcg agctgttcga ggccatgcgc gaggtggccg cgaccggccg ggggcccgac
     8701 cagcaactgc cctcggtggg ctgttcgatc aagtggcgcg actgaacata atggattgaa
     8761 ttcattgcag gtccggcgca gcgcaagccc tttcgcactt gccgctgcga cgtgattggg
     8821 cgaacatgcc gggtttagcc gccacgcgat tttccggagc cgagatgccc tcgcgcagag
     8881 atctcgccaa cgccatccgg gcgttgagca tggatgccgt ccagcaagcc aattccggcc
     8941 acccgggcat gccgatgggc atggcggaca tcgccgaggt cctctggaac gacttccacc
     9001 gccacaatcc cgccaacccg cgctgggcaa accgcgaccg tttcgtggtg tcgaacggcc
     9061 acggctcgat gctgctctac tcgctgctgc acctgtccgg ctacccgctg gccatggacg
     9121 agatccgtaa cttccgccag ttcggctgcc gtaccgccgg ccacccagaa tacgagccgg
     9181 ggctcggcat cgagaccacc accggtccgc tcgggcaggg cctggccaat gccgtcggca
     9241 tggcgatcgc cgagcgcgcc ctggcggcgc gctacaaccg gccgggcttc gaagtcatcg
     9301 accatcacac ctgggtgttc gccggcgacg gctgcctcat ggaaggcgtc tcgcacgagg
     9361 cctgttcgct ggccggcacg ctcggtctcg gcaagctgat cgtgttctac gacgacaacg
     9421 gcatctccat cgacggcgag gtcgagggct ggttcacgga cgacaccccg gcgcgtttcg
     9481 gcgcctacgg ctggcacgtg gtgccggggg tcgacggtca cgatccggcg gcggtcaggg
     9541 cggccatcga ggcggcccgc gccgagaccg ggcgaccgac gctcatctgc tgcaagaccg
     9601 tcatcgggtg gggcgcgccc aacaagcagg gcaccgaggc gacgcacggt gccgcgctgg
     9661 gcgcggacga ggtggcgcgc acgcgcgaga acatcggctg gccgcatccg gccttcgaga
     9721 ttccccagga gattcgtgcc ggctgggatg cgcgcgagcg tggcgcggcg gccgaggcgc
     9781 agtgggacac gctgctggcg gcctacgcaa aggagtatcc cgccgagagc gcggaactcg
     9841 tgcgccggct cgcgggcgag ttgccggccg gctggtcgca ggccgccgac gaggtgatcg
     9901 ccaggctggc cgaaagtgcc gccgacgtcg ccacgcgcaa ggcctcggag ctggcgctga
     9961 acggcttcgg caggctgctg ccggagctga tcggcggctc cgccgacctg agcggctcca
    10021 acaacaccat cttcaagggc agccggcgta tcggcgccgg cgaattcacc ggcaattacc
    10081 tgcattacgg cgtgcgcgaa ttcggcatga cggcgatcat gaacggcatg gcgctgcacg
    10141 gcggcttcaa gccctacggc ggcactttcc tggtgttctc cgactatgcg cgcaacgcag
    10201 tgcggatggc cgcgctgatg cgccagcccg tcacgctggt ctacacccac gattccatcg
    10261 gcctcggcga ggacggcccg acgcatcagc ccatcgagca cgtcgcgagc ctgcgcctga
    10321 tcccgaacct gcgcgtctgg cggccctgcg acctggcgga gaccgcggtc gcctggcgcg
    10381 acgcgctgga gagcacggcg acgcccacgg cgctggccct gtcccgccag aacctgccgg
    10441 cgcagccgcg caacccggag cagctggcgg ccatccggcg tggtggctac gtgctgcacg
    10501 agcccgcgag cgcgcccgcg gcgctgatca tcgccaccgg ctccgaggtc gacctcgcga
    10561 tgcaggcggc ggcactgctg ctcgcggagg gtgtcgcggt gcgtgtcgtc tccatgcctt
    10621 gcgtggagat ctttctggag caggacgccg cctggcgcga cgccgtgctg ccgccgcaca
    10681 tcacggcgcg cctcgcggtc gaggccggcg tgccggacgg ctggtgcccc ttcgtgggtg
    10741 gcgccggcgc cgtggtcgga atccggcgtt tcggcgagtc ggcacctgcc ggcaagttgt
    10801 tcgagcattt tggcttcacg ccccaggccg tcgccgcacg gctgcgggca ttgatcaagg
    10861 gttcctaagg agaaaggcaa tggcgatcaa ggttggcatc aacggctacg gacgcatcgg
    10921 caggaatatc ctgcgggcgc tctacgaatc ggggcgcacc ggcgagatca gcatcgtggc
    10981 gatcaacgat ctcggtgacg cgaacaccaa cgcccacctg acgcgctacg acaccgcgca
    11041 cggcaagttc agcggcaagg tcgaagtgga tggtgacagc ctggtggtca acggcgatcg
    11101 catccgcgtg ctggccgagc gcaacccggc caacctgccg tggaaagagc tgggcgtcga
    11161 ggtggtgctg gagtgcaccg gcctgttcac cagcaaggag aaggcctccg cgcatctcca
    11221 ggccggcgcg aagaaggtca tcatctccgc gccgggcggc aaggacgtcg acgcgaccat
    11281 cgtctacggc gtcaatcacg acaccctgaa gtcgaagcac gaggtcattt ccaacgcttc
    11341 gtgcacgacc aactgcctcg cgccgctggt gcagccgctg caccaggcga tcggcgtggt
    11401 caacggcctg atgaccacca tccatgccta caccaacgac caggtgctga ccgacgtcta
    11461 ccactccgac ctgcgccgcg cgcgc
//
LOCUS       DAOMPG010000057        11474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2430_length_11474_cov_4.471933, whole genome shotgun sequence.
ACCESSION   DAOMPG010000057 DAOMPG010000000
VERSION     DAOMPG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_11474_cov_4.471933"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..435)
                     /locus_tag="PLS34_04660"
     CDS             complement(130..435)
                     /locus_tag="PLS34_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86796.1"
                     /translation="MANNLAEVRMPDAVAAIRDAGFATWAGDSVAPALREKFHAARIP
                     VRNIRHVRVWGLQVDDERALPGRERTSIPDEDIWEISLEAKDGTTRSFDSRLLVAAN"
     gene            638..2695
                     /locus_tag="PLS34_04665"
     CDS             638..2695
                     /locus_tag="PLS34_04665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRP86797.1"
                     /translation="MRNSSWNITLLGITLLATGLSGCASVPAASVQAQPPELIPRQTL
                     LGNPFRASAQLSPDGTRIAFLAPREGVLNVWVADIGAPDQAVAVTDDRVRPIRNYFWS
                     PDSRQILYIQDKGGDENFLLYGVDLATGETRNYTPFENVRVFVVGMSTEIADEVLIGL
                     NDRDPRWHDVHRLNLHTGELRLERQNDGYAGFLADNDLRLRIAVRPAPDGSQVIEGID
                     AQGGMHTLTTVPAEDALTSGVLGIPRGADYAYMLDSRGRNTVSLVKLDVASGATEVIA
                     SGVRADIDGIMTHPASGELQAYSENYLVTRWLPVGDAVSADIEFLDRAARGEWTVTSR
                     SRDDRLWTLLIDRVSEPVAYWLYDREKRSLDKLFTIRPDLEAYTLAAMHPVEIASRDG
                     KTLVSYLSLPPHTDPERTGVPAEPLPLVLNVHGGPWARDSFGYRADHQWLANRGYAVL
                     SVNFRGSTGFGKDFVNAGDLEWGRRMHDDLLDAVEWAIERGITTREQVAIFGGSYGGY
                     AVLWGMTNTPDTFACGVDIVGPSNLKTLLDSVPPYWAAFFEQLARRVGDPRTEQGLAL
                     LAERSPLNYVDNIRKPLLIAQGANDPRVKQAESDQIVDAMMARGIPVTYVLYPDEGHG
                     FAVPANNLSFFAVAEGFLAACLGGRIEPVGDDFNGASIQVPTGAEHVPGLAEALQATE
                     TKQ"
     gene            2692..3237
                     /locus_tag="PLS34_04670"
     CDS             2692..3237
                     /locus_tag="PLS34_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRP86798.1"
                     /translation="MTTSRRLRQAWLAGAASMTLAAGCATTPKLYVDAADEPIECTTF
                     AWLEHPDRPASIGEQRLRAEVLRTLEARGYLEVTEDPDCLVSGVIYSGTKPGSPVNVG
                     VGAGRWGGSFGGSVGVSLPVGGGSKKVGNLTIDVIDVARNAQVWRGTLESAFATPEPA
                     SEDIAGAVGQVLEAFPRRGAD"
     gene            3264..4727
                     /locus_tag="PLS34_04675"
     CDS             3264..4727
                     /locus_tag="PLS34_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86799.1"
                     /translation="MRPYFPIIYVRGYAMRPADIAATVATPYMGFNLGATRARQDWRG
                     TVVRHVFESPLIRLMKEHGYRDVYQDGSEITGAIPPRSVIIYRYYEAADPELGTGKVP
                     SIEAAARGLRELIHQLRDQVCGTDAVARAEFRVHLVAHSMGGLVCRAFLQNDAIATAE
                     DRALVDKVFTYATPHNGIELAGMNVPAVLGLWDMNNFSRKRMAEYLDLPAGSERVDTL
                     NGRFPAERFFSLVGTNHRDYGLARIVAGEMSDGLVRIENATVRGAPRAFVYRSHSGPY
                     GIVNSEEGYQNLVRFLFGDVCMTGVLEPRRLPLPPSIRQSFETGRRVRASYYFEATVA
                     PRGAFMFKLSERRIETHSAVLRRFDELFGPAGAELAAPRSPVLFSTFLDTKKITAGRT
                     LVFSVDLAVSTTGYEVDGTRITRRHIPGEHLFRDTVTVRATRTAEGWNVRYVLADEQW
                     GESRGADAVRDGEDWLIPLQSAKGFEGRLRLSVRLRN"
     gene            complement(4769..5869)
                     /locus_tag="PLS34_04680"
     CDS             complement(4769..5869)
                     /locus_tag="PLS34_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86800.1"
                     /translation="MNGKSLNFRWLLVALLAATGVACSSNGGDDSGDGDDGEVVVLPR
                     YEFQMTSHPETPVVVQVPMGEGVSLRLTHLGDGPGVFGDFTLATGNFSIFGGSSLVIE
                     ELGEVPVLFGDLEIEATQTWVVPAAEVALSGAMEVRRGTERVFVTVLPAGAGVNVQWD
                     EDNNGTLDGEAALSWDEFEDLPDGAPEWQEMASFAYSAAIDFMLELAEWSITGIELID
                     DDLVTLSPIVAPCDAFSDAGLAVPPPPPVIPDEGFLTFTWYDDAASGSVNPGDSFSLG
                     FEYCWMDMTIFGEDYSSLYNGLIGMNSWTELVANNVLTRTGFEGMSPSGRAGGLVFED
                     FEIWETWVDGGNTVASQEAVVNGRLEVVFFAP"
     gene            complement(6072..8789)
                     /gene="ppc"
                     /locus_tag="PLS34_04685"
     CDS             complement(6072..8789)
                     /gene="ppc"
                     /locus_tag="PLS34_04685"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRP86801.1"
                     /translation="MTETPREPIHFEAKDEALRDDVREVGAIVGGVIAEQCGDAVFAA
                     VEQIRRAAILQRETGERGDLRTLLNSLDTRAATDVIRGFSSYFQVVNIAERVHRIRRR
                     REWLRDTRQLRSDSLEAAMRLLAEHRPDPADAKAFLDGLLIEPVFTAHPTEPTRRTIL
                     LKQQQIARRLVERMDASLTPQEHAALLANIRAQVTSAWQTEEHPETRITVGDEREQVL
                     FFLAESIYRVIPSVYERLEQAIAEVWGGDVDLPRPGIIFRFGSWVGGDMDGNPNVGPD
                     TIRETLALHRQTALDLYRAELQGVARELSQSPSRIGFSAEFTARLESYANDFPDALER
                     VAPRHRDMPYRVFCAQLARRLAATRAGDAGGYPDAETFIADLRLMAASLLDNKGANAG
                     LFAVNRLLWRAETFRFHLATLDLRQDSEVHRRCIAHCLGDERWLQRPRAERTARLQQL
                     LDEGAQCPLVDDEDAHKTLEVFRAAGEMLATYGERAIGPYIISMAQGADDVLSVLLLA
                     RWAGLASPDGVVPLDIAPLLETVDDLERGPEIVAELLDDRVYQAHLETRARRQVVMIG
                     YSDSNKDSGIAAARWALHQAEEALVAVAEQRGLDLTLFHGRGGTISRGGGRTHTAALA
                     APRGAVRGRLRVTEQGEIINAKYGLRGIAIRTLEQAASSVAEATLRDGGQPPPALWRE
                     IMEEIAEHSRQAYRALVHEDEGFYPYFRQATPIDVIERMNIGSRPASRRQMQGIRDLR
                     AIPWVFAWTQTRAILPGWYGVGTGLEAAIARHGEAPLKDMFGQWMFMRAMLDDLEMVL
                     AKCDMQIARRYAELADEPLQRYHETIHAEFRRTVDVVLRLKGAAELLENDATLSRTIR
                     LRNPYVDPMSLLQVDLLRRWREGGRRDEGLFRALLLSINGIAHGLQNTG"
     gene            complement(8794..9462)
                     /locus_tag="PLS34_04690"
     CDS             complement(8794..9462)
                     /locus_tag="PLS34_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP86802.1"
                     /translation="MIDLYTAPTPNGRKASIALEEMAIPYRVVAVDLAKGEQKSPEYL
                     AINPNGRIPAIVDRDAGDFAVFESGAILLWLAERCGRFLPDEAKARSVVLQWLFFQVG
                     GVGPMQGQANVFYRYAEEKIPYAIARYQREVRRLYEVYDRRLADHEYLAGDYSVADMA
                     TWPWIASHDWSGVSVDGLEHLQRWLRAVGDRPAVRRGMDVPKRWNPRPDVEKFVGVAR
                     KMLV"
     gene            complement(9455..9937)
                     /locus_tag="PLS34_04695"
     CDS             complement(9455..9937)
                     /locus_tag="PLS34_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRP86803.1"
                     /translation="MPSFDVVSELDGHEVANAVDQANREVDTRFDFKGTGAKFEVADF
                     VVTLEAQADFQLKQMMDILTQKLARRGIDVGCMKAEDPEIALNKARQKVVLRHGIESE
                     LARKIVKLVKDAKLKVQSQIQGDQVRVTGKKRDDLQEVIALLRTAELGLPLQFTNFRD
                     "
     gene            complement(9950..10279)
                     /locus_tag="PLS34_04700"
     CDS             complement(9950..10279)
                     /locus_tag="PLS34_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 pili tip component"
                     /protein_id="HRP86804.1"
                     /translation="MKIRELLNHWSGTRPPRLTAETYAIHLPVEEAARVHALAEMFPG
                     VPEEDILRDLLAAALAELEAAMPYEPGPKVASEDDFGDPVYEDAGPTPRFLELTRKHK
                     ERLAPEG"
     gene            10453..>11474
                     /locus_tag="PLS34_04705"
     CDS             10453..>11474
                     /locus_tag="PLS34_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86805.1"
                     /translation="MVDRDELRALAAGTPPAPARVVVLDVACEADGIDAPAGALSAAR
                     DAGCRTTLRLCGLPAAGDAGGVLAALAARLGREGGELAPEALALPAAVAHPEELALGA
                     VGACRGTPAVYMLLPHAALEAFQRRRPLALADGGEVAARRWWNGLLALAASAPAVSLV
                     PQAPGPGVSPLAPSQRWQGPSPGIGELIPAEPCRLRLVLDFGELLRACRDDLAGLARL
                     AERIVAMADELLEALPGAGGPRRLALELEGVARALVAAGRDPRSYASLGWLKRRLGAF
                     RAGARAASVRLARMRGGGLQPFPLPGALEVADADALDRALLVHGARHTHLACLSPWSL
                     APPEHGR"
BASE COUNT         1677 a         4040 c         3968 g         1789 t
ORIGIN      
        1 gggaccggcg ggaggtccgt ggcgcggcgc ggataaaaaa cggccgccag cccgatcacc
       61 acgaccgcca cgactatcca gagaattctg cgttgcatcg gggactccgg ccgtccggcc
      121 cttgcctgct cagttcgccg ccaccagcag gcgggaatcg aagctgcggg tcgtaccgtc
      181 cttcgcctcc aggctgatct cccagatgtc ctcgtccggg atgctggtgc gctcgcggcc
      241 gggcagggcc cgctcgtcgt ccacctggag cccccagacg cggacgtgac ggatgttgcg
      301 taccgggatg cgcgcggcat ggaatttctc gcgcagcgcc ggggctacgg aatcccccgc
      361 ccaggtagcg aaaccggcgt cgcgaatcgc ggcgaccgcg tcgggcatcc tgacttctgc
      421 taagttattg gccactggaa acactcctgg cgccaccctg gcaaatcgtg aacaggccgc
      481 gcaatggcct catcgccagc cggcccggac cacggtgaaa gattaacgcc cattgtgagg
      541 tgtcgccacc ccggtaagga tacgcgaccc gcaaatcgga aggagtcgtc cgcccgctgt
      601 cgcggctgac ggatcgaatg atggaaagag aggccacatg agaaactcgt catggaacat
      661 cacgctgctc ggaatcactt tgctcgctac cggtttgtca ggctgcgcct ccgtgccggc
      721 cgctagcgtg caggcgcaac cgccggagct gattccccgc cagacgctgc tcggcaatcc
      781 gttccgcgcc agcgcgcagc tcagcccgga cggtacccgc attgccttcc tggcgccgcg
      841 cgagggcgtg ttgaacgtct gggtggccga catcggcgcc cccgaccagg ccgtggcggt
      901 gaccgacgac cgggtccgcc cgatccggaa ctacttctgg tcgccggatt cgcggcagat
      961 cctgtacatc caggacaagg gcggcgacga gaacttcctg ctctacggcg tcgacctcgc
     1021 cacgggcgag acccgcaact acacgccctt cgagaacgtc cgggtcttcg tggtcggcat
     1081 gagcaccgag atcgcggacg aagtcctcat cggcctcaac gatcgcgacc ctcgctggca
     1141 cgacgtgcac cgactgaatc tccacaccgg cgaactgcgc ctcgagcgcc agaatgacgg
     1201 ttacgccggc ttcctggcgg acaacgacct gcggctgagg atcgccgtgc ggccggcccc
     1261 ggacggcagc caggtcatcg aaggcatcga tgcgcagggc gggatgcaca cgctgaccac
     1321 cgtgccggcc gaggacgcgc tgaccagcgg ggtgctcggc attccgcgcg gcgcggacta
     1381 cgcctacatg ctcgactcgc gcggccgcaa caccgtgtcg ctggtaaaac tggacgtggc
     1441 ctccggcgcc accgaagtga tcgccagcgg cgtcagggcc gacatcgacg gcatcatgac
     1501 gcacccggcg agcggcgagc tgcaggccta ctcggaaaac tacctggtca cgcgctggct
     1561 cccggtcggc gacgcggtca gcgcggacat cgagtttctc gatcgggcgg cccgcggcga
     1621 gtggaccgtc accagccgca gccgcgacga tcgcctgtgg acgctgctga tcgaccgggt
     1681 cagcgagccg gtggcctact ggctgtacga tcgcgaaaag cgcagcctcg acaaactgtt
     1741 caccatccgc ccggatctcg aggcctacac gctggcagcc atgcacccgg tggagatcgc
     1801 cagccgcgac ggcaagacgc tggtcagcta tctctcgctg ccgccgcata ccgatcccga
     1861 gcgcaccggc gtccccgccg agcccctgcc gctggtgctg aacgtgcacg gcggtccctg
     1921 ggcccgcgac agcttcggct accgcgccga ccaccagtgg ctggccaacc gtggctacgc
     1981 ggtgctgtcg gtgaactttc gtggctccac cggcttcggc aaggacttcg tcaacgcggg
     2041 cgacctcgag tggggccgca ggatgcacga cgacctgctc gacgccgtcg agtgggccat
     2101 cgagcgcggt attaccacgc gcgagcaggt ggcgatcttc ggcggttcct acggcggcta
     2161 tgccgtgctg tgggggatga cgaacacgcc cgacaccttt gcctgcggcg tggatatcgt
     2221 cggcccgtcg aacctcaaga cgctgctcga cagcgtgcca ccctactggg cggcgttttt
     2281 cgagcagctg gcgcgccggg tcggcgaccc gcgtaccgag caaggcctgg cgctgctcgc
     2341 ggagcgctcg ccgctcaact acgtggacaa catccgcaag ccgctgttga tcgcccaggg
     2401 ggccaacgac ccgcgcgtca agcaggcgga gagcgaccag atcgtggacg ccatgatggc
     2461 ccgcggcatc ccggtgactt atgtgctgta ccccgacgag gggcacggct tcgcggtgcc
     2521 ggcgaacaac ctcagcttct tcgccgtggc ggagggcttc ctggccgcct gcctgggcgg
     2581 ccgcatcgag ccggtgggcg atgatttcaa cggcgccagc atccaggtgc cgacgggggc
     2641 cgagcacgtg cccggcctcg cggaggcgct gcaagcaacg gagaccaagc aatgacgacc
     2701 tctcgaaggc tgcgccaggc ctggctggcg ggcgcggcat cgatgacgct ggcggcgggc
     2761 tgtgcgacca cgccgaagct ctacgtcgat gcggcggatg aaccgatcga atgcaccacc
     2821 ttcgcgtggc tcgagcaccc cgaccggccg gcctcgatcg gcgagcagcg cttgcgcgcc
     2881 gaagtgctgc ggacgctgga ggcgcgcggc tacctcgaag tcaccgagga cccggactgc
     2941 ctcgtgtcgg gcgtcatcta cagcggcacg aagcccggct cgccggtcaa cgtcggcgtc
     3001 ggcgccggcc gctggggcgg cagcttcggc ggcagcgtcg gcgtcagcct gccggtcggc
     3061 ggtggctcga agaaggtcgg caacctgacc atcgacgtga tcgacgtggc gcgcaacgcg
     3121 caggtctggc gcggcacgct ggagtcggcg ttcgccaccc cggagccggc gtccgaggac
     3181 atcgccggcg cggtcgggca ggtgctggag gcctttccgc gccgcggcgc ggactgacgc
     3241 cgggtcggga cgggaggctg cgcgtgcgcc cgtacttccc gatcatctac gtgcgtggct
     3301 atgcgatgcg gccggcggac atcgccgcaa ccgtggccac gccgtacatg ggcttcaacc
     3361 tgggcgccac cagggcacgg caggactggc gcggcacggt cgtccggcac gttttcgagt
     3421 cgccgctcat ccggctgatg aaagagcacg gctaccgcga cgtctaccag gacggcagcg
     3481 agatcaccgg tgcgattccg ccgcgtagcg tgatcatcta tcgctactac gaggccgcgg
     3541 acccggagct cggcaccggc aaggtgccgt cgatcgaagc ggcggcgcgc ggcctcaggg
     3601 agttgatcca ccagcttcgc gaccaggtct gcggcaccga tgcggtggcg cgcgccgagt
     3661 tccgcgtcca cctcgtggcg cattccatgg gcggactggt gtgtcgcgcc ttcctgcaga
     3721 acgacgccat cgccacggcc gaggaccgag cgctggtcga caaggtgttc acctacgcca
     3781 cgccgcacaa cggcatcgag cttgccggca tgaacgtgcc ggccgtgctg ggcctgtggg
     3841 acatgaacaa cttcagccgc aagcgcatgg cggagtatct cgacctgccg gccggatccg
     3901 agcgcgtcga tacgctgaac ggccgcttcc cggcggaacg cttcttcagc ctggtcggca
     3961 ccaatcatcg cgactacgga ctggcgcgca tcgtggccgg cgagatgagc gacggcctgg
     4021 tgcgcatcga gaacgccacg gtgcgcggcg cgccgcgcgc cttcgtctac cgcagccaca
     4081 gcggccccta cggcatcgtc aactccgagg agggttacca gaacctggtg cgcttcctgt
     4141 tcggcgacgt ctgcatgacc ggcgtgctcg agccgcgccg cctgccgctg ccgccgagca
     4201 tccggcagtc cttcgagacg gggcggcggg tgcgtgcctc gtactacttc gaagccaccg
     4261 tggcgccgcg cggcgcattc atgttcaagc tgtccgagcg acgcatcgag acccactcgg
     4321 cggtcctgcg ccgcttcgac gagctgttcg gcccggccgg tgccgagctc gccgcgccgc
     4381 gctcgccggt gctgttctcc accttcctcg acacgaaaaa gatcaccgcc ggtcgcaccc
     4441 tggtgttcag tgtggacctg gccgtctcga ccaccggcta cgaggtggac ggcactcgca
     4501 tcacgcgtcg gcacatcccc ggcgagcacc tgttccgtga cacggtgacg gtgcgcgcca
     4561 cccggaccgc agaaggctgg aacgtgcgct acgtgctggc cgacgagcag tggggcgagt
     4621 cacgcggcgc cgacgcggtg cgcgacggtg aggactggct cattccgctg cagtccgcca
     4681 agggtttcga gggccggctg cggctcagcg tgcggctgcg gaactgaagt gcgcccgcga
     4741 gcgaactcgc gggcgcgcaa tcacggtgct acggtgcgaa gaacaccact tccagccggc
     4801 cgttcaccac ggcctcctgc gaggcgacgg tattgccgcc gtccacccag gtctcccaga
     4861 tttcgaagtc ctcgaacacc aggccgccgg cccggccgct ggggctcatg ccctcgaagc
     4921 cggtccgcgt cagcacgttg ttagcaacca gctcggtcca gctgttcatg ccgatcaggc
     4981 cgttgtacag cgagctgtag tcctcaccga agatcgtcat gtccatccag cagtattcga
     5041 agcccaggct gaaactgtcg cccgggttca cgctgccgct ggccgcatcg tcgtaccagg
     5101 tgaaggtcag gaaaccctcg tccggtatga ccggcggcgg gggcggcacg gccagcccgg
     5161 cgtcgctgaa ggcgtcgcag ggcgcgacga tcgggctcag cgtgaccaga tcgtcgtcaa
     5221 tcagctcgat gccggtgatg ctccattccg ccagctcgag catgaagtcg atggctgcgg
     5281 agtaggcgaa gctcgccatc tcctgccatt cgggcgcgcc gtccggcagg tcctcgaact
     5341 cgtcccagct cagcgccgcc tcgccgtcca gcgtgccgtt gttgtcctcg tcccactgca
     5401 cgttgacgcc cgcgccggcc ggcagcacgg ttacgaatac gcgctcggtc ccgcgccgca
     5461 cctccatcgc cccggagagc gcgacttctg ccgcgggcac gacccaggtc tgggtcgcct
     5521 cgatctccag gtcgccgaac agcaccggca cttcgccgag ctcctcgatg accaggctcg
     5581 agccgccgaa aatcgagaaa ttgccggtcg ccagggtgaa atcgccaaac acgcccggac
     5641 cgtcgccgag gtgcgtgagc cgcaggctga cgccttcgcc catgggtacc tgcacgacga
     5701 ccggagtctc agggtgactg gtcatctgga attcgtagcg cggcaggacc acgacttcgc
     5761 cgtcgtcgcc gtcgccgctg tcgtcaccac cgttgctgct gcaggccacg cccgtcgcgg
     5821 cgagcagggc gaccagcagc cacctgaaat tcaatgattt gccgttcatg gcagccatcc
     5881 tcgtttccca ttgccgagga aacggctgga tcgtcaaccg ggggggggct gccagaggtg
     5941 tccgtgtatg ccggggcgcc agaggtgtcc ttgaggccga tttcccagcc cgtctccaac
     6001 tccgccctgc acggggcgca gggcgaccct gaacataatc tagtccagct ttggcgaaac
     6061 gccagcccgt ttcagccggt gttctgcaag ccgtgggcga tgccgttgat cgacagcaac
     6121 agggcgcgga acagcccttc gtcccggcgt cccccctcgc gccagcgccg cagcaggtcc
     6181 acctgcagca ggctcattgg atcgacgtag ggattgcgga ggcgaatggt gcggctcagc
     6241 gtggcgtcgt tctcgagcaa ctcggcggcg cccttcaggc gcaacacgac gtcgaccgtg
     6301 cgccggaatt ccgcgtggat ggtttcgtgg taacgctgca agggctcgtc cgccagctcg
     6361 gcatagcggc gcgcgatctg catgtcgcac ttcgccagca ccatctcgag gtcgtccagc
     6421 atggcgcgca tgaacatcca ctggccgaac atgtccttga gcggcgcctc gccgtggcgc
     6481 gcgatggcgg cctcgagccc ggtgccgacg ccgtaccagc cgggcaggat ggcgcgggtc
     6541 tgggtccagg cgaacaccca gggaatggca cgcaggtcgc ggatgccctg catctgtcgc
     6601 cgcgaggcgg gccgcgatcc gatgttcatc cgctcgatca cgtcgatggg cgtcgcctgg
     6661 cggaagtagg ggtagaagcc ctcgtcctcg tgcaccagcg cgcgatacgc ctggcggctg
     6721 tgctccgcga tctcctccat gatctcgcgc catagggcag gcggcggctg gccgccgtcc
     6781 cgcagggtgg cctccgccac cgagctggcc gcctgctcca gggtgcggat ggcgatgccg
     6841 cgcagcccgt acttggcgtt gatgatctcg ccctgctcgg tgacccgcag gcggccgcgc
     6901 accgcgccgc gcggcgccgc cagcgccgcc gtgtgggtgc ggccgccgcc gcggctgatg
     6961 gtgccgccgc ggccgtggaa cagcgtcagg tccagcccgc gctgctccgc caccgccacc
     7021 agcgcttctt ccgcctggtg caacgcccag cgcgcggcgg cgatgcccga gtccttgttc
     7081 gagtcggagt agccgatcat caccacctgg cggcgcgcgc gggtctccag gtgcgcctgg
     7141 tagacgcggt cgtcgagcag ttcggcgacg atctccgggc cgcgctccag gtcgtcgacc
     7201 gtttccagca gcggcgcgat gtcgagcggc acgacgccgt ccgggctcgc cagcccggcc
     7261 cagcgcgcca acagcagcac cgacagcacg tcgtcggcgc cctgcgccat ggagatgatg
     7321 tacgggccga tggcgcgctc cccgtaggtc gccagcatct cgccggcggc ccggaacact
     7381 tccagcgtct tgtgggcatc ctcgtcgtcg accagcgggc actgcgcgcc ctcgtcgagc
     7441 agctgctgca ggcgcgcggt gcgctcggcg cgcggccgct gcagccaccg ctcatcaccc
     7501 aggcagtgcg cgatacagcg ccggtgcact tccgaatcct ggcgcaggtc gagcgtggcg
     7561 aggtggaagc gaaaggtctc ggcccgccac agcagccggt tgaccgcaaa caggccggcg
     7621 ttggcgccct tgttatcgag caggctcgcg gccatcagcc gcaggtcggc gatgaaggtt
     7681 tcggcgtcgg ggtagccgcc ggcgtcgccg gcgcgggtcg cggccagccg ccgcgccagt
     7741 tgcgcgcaga acacccggta gggcatgtcc cgatgccgcg gggccacccg ctcgagcgcg
     7801 tccgggaagt cgttcgcgta ggactccagc cgggccgtga actccgccga gaagccgatc
     7861 cggctgggcg actgcgacag ctcgcgtgcc acgccctgca gctcggcgcg gtacaggtcg
     7921 agggccgtct ggcggtgcag cgccagggtc tcgcggatgg tgtcggggcc gacgttcggg
     7981 ttgccgtcca tgtcgccgcc gacccagctg ccgaagcgga agatgatccc cggccgcggc
     8041 aggtcgacgt ccccgcccca cacctcggca atggcctgtt ccaggcgctc gtacaccgac
     8101 gggatgaccc ggtagatgct ctcggcgaga aagaacagca cctgctcgcg ctcgtcgccc
     8161 acggtgatgc gggtttccgg gtgctcctcg gtctgccacg ccgaggtgac ctgcgcgcgg
     8221 atgttggcga gcagcgccgc gtgctcctgc ggcgtgagag aggcgtccat gcgctccacc
     8281 aggcgccgcg cgatctgctg ctgcttgagc aggatggtgc gccgcgtggg ttcggtcgga
     8341 tgtgccgtga acaccggctc gatcagcagt ccgtcgagga aggccttggc atcggccggg
     8401 tcgggccggt gctccgcgag caggcgcatg gccgcctcga ggctgtcgga gcgcagctgc
     8461 cgcgtgtcgc gcagccactc gcgccggcgc cggatgcggt gcactctctc cgcgatgttg
     8521 accacctgga agtagctcga aaagccgcgg atgacgtcgg tcgcggcccg cgtgtcgagc
     8581 gaattgagca gtgtgcgcaa atcgccccgc tcgccggtct cgcgctgcag gatggcggcg
     8641 cggcggatct gctcgacggc cgcgaacacg gcgtcgccgc actgttccgc gatgaccccg
     8701 ccgacgatgg cgccgacctc gcgcacgtcg tcgcgcaacg cctcgtcctt ggcctcgaaa
     8761 tggatcggct cgcgcggcgt ctccgtcacg gtgtcagacc agcatcttgc gcgcgacgcc
     8821 gacgaacttc tcgacgtcgg gcctcgggtt ccagcgcttc ggcacgtcca tgccgcgccg
     8881 gaccgccggg cggtccccga ccgcgcgcag ccagcgctgc aggtgttcaa gcccgtccac
     8941 ggacacgccg gaccagtcgt gcgaggcgat ccacggccag gtggccatgt cggcgacgga
     9001 gtagtcgccc gcgaggtact cgtggtccgc caggcgccgg tcgtacacct cgtagaggcg
     9061 gcgcacctcg cgctggtaac gcgcgatggc gtagggaatc ttctcctcgg cgtagcggta
     9121 gaacacgttc gcctggccct gcatcgggcc gacgccgccg acctggaaga acagccactg
     9181 cagcaccacc gagcgggcct tcgcctcgtc cggcaggaag cgtccgcagc gctccgccag
     9241 ccacagcagg atggcgcccg actcgaatac cgcgaagtcg ccggcgtcgc ggtccacgat
     9301 ggccgggatg cggccgttgg gattgatggc gaggtactcg ggcgacttct gctcgccctt
     9361 cgccaggtcc accgccacca cgcgatacgg gatggccatt tcctcgagcg cgatggaggc
     9421 cttgcgcccg ttgggcgtcg gcgcggtgta gagatcaatc acggaagttg gtgaactgca
     9481 gcggcaggcc gagctccgcc gttctcagca gcgcgatcac ctcctgcagg tcgtcgcgct
     9541 tcttgccggt gacgcgcacc tggtcgccct ggatctgcga ttgcaccttg agcttggcgt
     9601 ccttcaccag cttgacgatc ttgcgcgcca gttcggactc gatgccatgg cgcagcacga
     9661 ctttctggcg cgccttgttg agcgcgattt ccgggtcctc ggccttcatg cagccgacgt
     9721 cgatgccgcg ccgggccagc ttctgcgtca ggatgtccat catctgcttg agctggaagt
     9781 cggcctgggc ctcgagggtg acgacaaagt cggcaacctc gaatttcgcc ccggtcccct
     9841 tgaagtcgaa acgcgtgtcc acctcgcggt tggcctggtc caccgcgttg gccacttcgt
     9901 ggccgtcgag ttccgatacg acgtcgaatg aaggcatgtc tcgttcgcct cagccctccg
     9961 gggcaaggcg ctccttgtgc ttgcgggtga gttccaaaaa gcgcggcgtc ggccccgcgt
    10021 cctcgtagac cgggtcgccg aagtcgtcct cgctggcgac cttcgggccc ggctcgtagg
    10081 gcatcgccgc ctcgagctcg gcgagcgccg ccgccagcag gtcgcggagt atgtcttcct
    10141 cgggcacgcc cgggaacatc tcggccagcg catgcacgcg cgcggcctct tcgaccggca
    10201 ggtgaatcgc gtaggtctcc gcggtcagcc gcggcgggcg agtgcccgac cagtgattca
    10261 gcagttccct gattttcacg cgcacctcct gtcgttcccc gaatgatggt gtgccgggcc
    10321 ggtcgctgca aggcggcgcg gcgtgccgct ggacgttttg cccgctattg gccgctttct
    10381 tccccttacg ccgtggtggt ggcgcgcaca gactgggcga cgcgggccat ggacgcgcag
    10441 ggagaccagg gaatggtgga ccgagacgaa cttcgggcgc tggcggcagg gacgccgccg
    10501 gcgcccgccc gggtggtggt gctcgacgtc gcctgcgaag ctgacggcat cgacgcgccc
    10561 gcgggggcgt tgagcgcggc gcgcgacgcc ggctgccgca ccacgctgcg cttgtgcgga
    10621 ttgccggcgg cgggcgatgc cggcggtgtg ctcgccgcgc tggccgcgcg gctgggccgc
    10681 gaaggcggcg agctggcgcc ggaggccctg gcgctgccgg ccgcggtggc acacccggag
    10741 gaactcgcac tcggcgccgt cggcgcctgt cgtgggaccc cggccgtcta catgctgctg
    10801 ccgcacgcgg cgctcgaggc gttccagcgg cggcgtccgc tggcgctcgc cgacggtggc
    10861 gaagtcgcgg cgcggcgctg gtggaacggc ctgctggccc tggccgccag cgcgccggcc
    10921 gtcagcctgg tgccgcaggc ccccggcccg ggcgtctcgc cgctggcgcc gtcgcaacgc
    10981 tggcagggcc cctcgccggg catcggcgag ctcatccccg ccgagccctg ccggctccgg
    11041 ctggtgctcg actttggcga gctgttgcgt gcctgccgcg acgaccttgc cggactggcg
    11101 cggctcgcgg agcgcatcgt ggccatggcg gacgaattgc tggaggcgct gccgggcgcc
    11161 ggcggtccgc gtcgcctggc gctggagctc gagggcgtgg cgcgggcgct ggtcgcggct
    11221 ggccgtgatc cgcgcagcta tgcctcgctg ggctggctga agcgtcggct cggcgccttc
    11281 cgcgccgggg cgcgcgcggc atcggtgcga ctggcgcgca tgcgcggcgg cggcctgcag
    11341 cccttcccct tgccgggcgc gctggaggtg gccgacgccg acgcgctgga tcgcgcactg
    11401 ctggtgcacg gtgcgcgcca tacccacctg gcctgcctgt cgccctggag cctggcgccg
    11461 ccggagcacg gtcg
//
LOCUS       DAOMPG010000058        11404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2452_length_11404_cov_3.591242, whole genome shotgun sequence.
ACCESSION   DAOMPG010000058 DAOMPG010000000
VERSION     DAOMPG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_11404_cov_3.591242"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLS34_04710"
     CDS             complement(<1..853)
                     /locus_tag="PLS34_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP86806.1"
                     /translation="MTESFWPDLLVSRDPREVLLQSPGRDWSAGEVLAEVRALQPRLA
                     AARVAAVLADNGPAWIIADLACQSVGVAHLPLPAFFSASQLRHALEQTGADTVLTDQP
                     ARIGALDLDFGITGHWQGLTWMRRVVSPAPLPPGTAKIAFTSGSTGAPRGVCLAAAGL
                     LDTAHAVCTRLHDLSPAGHLAVLPLALLLENVAGVYAPLLRGLPVHLRPLAELGWQGM
                     ASFDPECLDRLARSTGAGSVVLVPELLKAWTTWLARTGAAAPATLRFAAVGVARVDVP
                     VVRVVGPE"
     gene            complement(846..1445)
                     /locus_tag="PLS34_04715"
     CDS             complement(846..1445)
                     /locus_tag="PLS34_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HRP86807.1"
                     /translation="MAAIQHAARAVMRGLVPRHTITGSPRRAACEAFISGIFRRRHGA
                     VLQHFAPNLVLLERGGRIAAAAGWRSAADEALFLECYLDEPVEAAVGRVAGHAVSRAR
                     IVEVGHLASDTPGGTVAVILALADRLDRLGFEWVVFTATDELIAIFRRLGLPPLAIAA
                     ADQRRLGEQADRWGRYYDGRPVVVAGRIRLALERAVCRD"
     gene            1609..2907
                     /locus_tag="PLS34_04720"
     CDS             1609..2907
                     /locus_tag="PLS34_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86808.1"
                     /translation="MSTSLRLFTLPVIGGIAVLALLAWSAPAPAMPAFARQYNVSCQM
                     CHSAFPRLNEFGEYFAAENMRLPNWRDTTMDAGDDRLALPKSLPLAIRAQAYVQGRDS
                     EEVDYATGPTGNDSSFDVQAPYLVKLLSSAPLSDNITYYFYGIFAEKGGNGEVIIEDA
                     WFSYADIAGSGVGGMLGQFQISDLMFPREVRLTFQDYYVYRSAGITYDRGIILDRAFG
                     PVDLAFGLTNGNGIQENVTINSPGYKRPDHMFDNDSGKSLFGRIGTTVGPVNVGLFGL
                     VGEQRSAAGFAGADRGSRDTDKTIYGLDFSGAFNARTHWYAQLLWNRWDEFLDADPTA
                     DYDWFGGFAGVDFIMNDRWALSLLYNYADASDFDGTGTVFEGIDINSLTFGAAYYFMR
                     NVKAVIELNVDLLSEQTGGPPFVGHQSKENYLLVGFDAAY"
     gene            complement(2966..3703)
                     /locus_tag="PLS34_04725"
     CDS             complement(2966..3703)
                     /locus_tag="PLS34_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRP86809.1"
                     /translation="MTKYNSRTPLLAAALAGGMMLAAPAAVHAEDGWWIGGSLTATSD
                     YVFRGVSQTDEGPAVQAGLDIGYGAGFYAGIWASNVDFDQPDGIDMELDFFVGYVFGL
                     TDDATLDLSVARYTYPGSNPGFDIDYNEFTAILGFAERYSATVAYANDYVNTGESAFF
                     YQLAADAPLGETGFNFRLAAGFNDISRAAGSDYWDYQLGINRSWGNFSADLSYFDTSG
                     YDSDVEAFLGPGKWADGRVVLTLGVTF"
     gene            complement(3755..5281)
                     /locus_tag="PLS34_04730"
     CDS             complement(3755..5281)
                     /locus_tag="PLS34_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HRP86810.1"
                     /translation="MSEQPHQNTGRVNEVVDRVGRIENVGVEYIPEDQRRSELINVFW
                     ILFGGSMTFGIIIIGWIPVSLGLGWWEALSAIVVGTAVGSFLLAPMSLFGPRTGTNNP
                     VASGAHFGAKGRLIGSLLGVTACIVFAALCVWAAGDVLAGSVARVTGTEDSQLELQLL
                     CYALVSIVMVGIAVLGHANMVVFCKLMVPTAGVLMVVGIFAYYPTFDASYQPGDYALG
                     SFWPTWIAGAIVCAATTNSYGPYAGDWTRHVSRKRYSDRQLMVVTWLGGFIGMGGAYV
                     WGAYTSASFADPTMDYALGLVVNAPTWYLLPLLYIGLVAGTAQAVINIYSMGLDFSAI
                     VTRFSRVECTIGLGLISTLLVYIGAFYEQVALLVTSFLAILVILGAPWVVVNIVGLIS
                     RRGYYYADHLQVFNRGEIGGRYWFAGGLNWQATTAWALGVAAGSLFINTGWYVAPGAA
                     LLGGADTGLFVSSFVSAAAYILLLYRFPEPAAAYGPPGAYFRHDGRPEFPEIRPKNGR
                     "
     gene            complement(5459..6814)
                     /locus_tag="PLS34_04735"
     CDS             complement(5459..6814)
                     /locus_tag="PLS34_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HRP86811.1"
                     /translation="MDTLRPVDPSHEEVVRSLAEQGVGYVLGSWVDILGRARAKATPI
                     KRLPELLGGFARYTPRGICGIGTMNPVEEEVTSLPDLDTLTVLPWDTRFAWMAADMWS
                     DKGEPFELCPRSILKRQVEKAREQGYTATLGVEPEFYVFRPESLGKEASHLIPVTPSA
                     YVKPSPAYDVETLLDTADFLQRLVEYMPGIGLEAYGMGVEGGEGQYEIDFYYKSVLEM
                     ADRMVLFRLMTHQVAKQFGLCATYMPKPYETVWGSGNHFNMGLYHATGEQKSALRDGK
                     RFTAECRAFTSGILKHARALTALSNPLVNSYRRLVPRLMDGSVSWAPIKISYGHNNRS
                     CMIRWPENRPCIENRSVDPAANPYLVAAFMLAAGMEGIRENLDAGPPNDTLTYADRNI
                     ANLPRSLVDAVEAFEQDPLVHEVFAPGFIRDYVDTKRQEWEQANLPVPQRERDEYLLH
                     V"
     gene            6962..8236
                     /locus_tag="PLS34_04740"
     CDS             6962..8236
                     /locus_tag="PLS34_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRP86812.1"
                     /translation="MKVANATPSERTQQTPNETAFRNMTGAEPVLLDVAPAIDVIPGY
                     QRNVILTSGPPLPWDQYYGGQRDAVIGAALYEGLARNAEEADALIARGGIRLAPCGEF
                     DCVGSLAGVYSASMPVFVVQNKTHGNFAYCNVYEGSNPRRLNYGVYDEGVRARLNHIG
                     MAVAPVIGAAVRAAGEIPLKPIMKRALHMGDELHSRNTAASLLFGRELFPYLLELREE
                     RAARVAETVRVLTEDHYFFLRLSMAAAKTMGDAAHGVPGSSLVSAMAFNCRHFGIRVS
                     GLGERWFLGPHPEVHARLFDGKTEADITWMGGESTITETVGIGGFAQAAAFPLQAYQG
                     GSPEAMVDNNVSMYRITHAEHPDFRIPFLAYRGTPTGIDVLKVLETGVTPVMDLGIAG
                     KGGGQIGAGLVRAHIGCFEAAAREFRAQYGET"
     gene            8303..8749
                     /locus_tag="PLS34_04745"
     CDS             8303..8749
                     /locus_tag="PLS34_04745"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HRP86813.1"
                     /translation="MLSNSYFWQSGLVFLIGLVPFLEIYVAIPAGLAMGLDPVSSFAW
                     ATLGNCLPLALVPRFHDWVHRHERLGGWIDRFISEKWERRFNRYGKGALLAFTPWVGV
                     WATAASAAALRVAPNVIVFWGCLSIVLYGLGTVLLGVGVMSFFGGD"
     gene            8774..9688
                     /locus_tag="PLS34_04750"
     CDS             8774..9688
                     /locus_tag="PLS34_04750"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRP86814.1"
                     /translation="MDLQRVLTEVADMATPYFGRGALPDYIPALAAVDPRQFGMAVMT
                     VSGDEHAVGDVDTPFSIQSISKLLSLILAMNLEGELLWSHVGREPSGTRFNSLVQLEY
                     ERGVPRNPFINAGALVTLDRVLPRVHDYRTLLLDFVRFLACSNAIDYDASVVASERDG
                     AHVNFALAHLLKSYHRIDSPVDALLDAYFHQCALAMSCRQLARTFLALANRGLSPLVE
                     EAVVTARQAKRINSLMLTCGMYDSVGSFAFRVGLPAKSGVGGGIVAVVPDRMCIATWA
                     PELDLSGNSYVGVLALERFTEITGLSVF"
     gene            complement(9692..10357)
                     /locus_tag="PLS34_04755"
     CDS             complement(9692..10357)
                     /locus_tag="PLS34_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP86815.1"
                     /translation="MARKTKPQGVGPAGTRKRAGKEVRREQLLEAALDLFARRDFATV
                     TNKEISAAAGMNSGLIYYYFESKEDLFRSALEFAVGRLLDEYRTIRDGDRDPVARIRH
                     WFHINTEMSETILQVIKIMIDYHGMVDSEFSIDDLIAHFYHQEEKGILADSIREGIRL
                     GLFRKVNAARTARFVSIHLDGIMAAHLARGPLDVRRSLKDLEDFLWSHLGYEPAAGRV
                     ARR"
     gene            10596..10670
                     /locus_tag="PLS34_04760"
     tRNA            10596..10670
                     /locus_tag="PLS34_04760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10628..10630,aa:Val,seq:cac)
     gene            10845..11192
                     /locus_tag="PLS34_04765"
     CDS             10845..11192
                     /locus_tag="PLS34_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86816.1"
                     /translation="MSQAIDPSKSDAAAAIDEVLVAETAVREAMEACRQEAERVLDAA
                     REDARRIARIASQRASRLHARCDQLVAGQIEEIRRAAGVDAPRTELNAEDRERLAAAV
                     ARLAARLTRPSDG"
     gene            11185..>11404
                     /locus_tag="PLS34_04770"
     CDS             11185..>11404
                     /locus_tag="PLS34_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86817.1"
                     /translation="MADDARLAYLQAGLQARLGDRPAADERRVAEASIDLSHYLEALR
                     RSSLKRWIGDLNPDMAPEAVERQLRAAWR"
BASE COUNT         1885 a         3922 c         3765 g         1832 t
ORIGIN      
        1 cctccgggcc aactactcgt acaactggta catcgacccg cgcaacgccg acggccgcga
       61 agcgcagggt ggccggtgcc gcggcgccgg tccgcgcgag ccatgtcgtc caggccttga
      121 gcagctccgg gaccagcacg acgctgccgg cgccggtgct gcgcgccagc cggtcgaggc
      181 attccggatc gaacgaagcc atgccctgcc agccgagctc ggccagtggc cgaaggtgca
      241 ccggcaggcc gcgcagcagc ggcgcataga cgccggccac gttctccagc agcagcgcca
      301 gcggcagcac cgcgaggtgc ccggccggtg acaagtcgtg cagccgcgta cacactgcat
      361 gggcggtgtc gagcaacccg gcagccgcga gacacacgcc cctgggcgcc ccggtgctgc
      421 cggaggtgaa ggcaatcttg gcagtgccgg gcggcagcgg tgccggcgac acgacgcggc
      481 gcatccaggt cagcccctgc cagtgtccgg tgatgccgaa gtcgaggtcc agcgcgccga
      541 tccgggcggg ctggtcggtc agcacggtgt cggcgccggt ttgctcgagc gcatgccgca
      601 gctgcgacgc gctgaagaac gcgggcagcg gcaggtgcgc gacacccact gactggcagg
      661 cgagatccgc gatgatccag gccggcccgt tgtcggcgag cacggccgcc acccgcgcag
      721 cggccagccg tggctgcagc gcacggactt cggcgaggac ttcgccggcc gaccagtccc
      781 gcccgggtga ctgcagcagt acttcgcgcg gatcgcgcga caccagcagg tccggccaga
      841 aggactcagt cacgacacac cgccctctcc agcgccagcc ggatgcggcc ggcgaccacc
      901 accggccggc cgtcgtagta ccggccccag cggtccgcct gctcaccgag ccggcgctga
      961 tccgcagccg cgatcgccag cggcggcagc cccagccgcc ggaatatggc gatcaactcg
     1021 tcggtcgcgg tgaacaccac ccactcgaag ccgaggcgat cgagacggtc ggccagcgcc
     1081 aggatgacgg ccacggtgcc gcccggggtg tcggacgcca ggtggccgac ctcgacaatg
     1141 cgcgcacggc tgaccgcatg gcccgcgacg cggccgactg cagcttccac cggctcgtcc
     1201 aggtaacact ccaggaacag cgcttcgtcg gccgcgctgc gccagccggc ggctgccgca
     1261 atccgcccgc cgcgctcgag caggaccagg tttggtgcga agtgctgcaa caccgcgccg
     1321 tggcggcgtc gaaagatccc gctgatgaac gcctcgcagg cggcgcgccg cggcgatccg
     1381 gtgatggtat gcctggggac caggccgcgc atcacggccc gcgcggcatg ttgaatggca
     1441 gccaagtcga cgttcccctg cagttgaccg gatgcagagg ctagccgtgc tcgcttaagc
     1501 cagtcttaag ggctcttgac cagaatcaat gtccgcccgg cggcgttgcg ctttacttgc
     1561 ggcagccgtc gcctccatca gacccgcgga tttacccagg agcgcgccat gagcacttcc
     1621 ctgcgcctgt tcaccttacc cgtcatcggc ggaatcgccg tgctcgcgct gctcgcctgg
     1681 tcggccccgg ccccggccat gcccgccttc gcgcgccagt acaacgtcag ctgccagatg
     1741 tgccacagcg cctttccgcg cctcaacgag ttcggcgagt atttcgccgc tgagaacatg
     1801 cggctgccga actggcgcga cactacgatg gacgccggcg acgaccggct ggcgctgccc
     1861 aagagcctgc cgctggcgat tcgcgcgcag gcctacgtgc aggggcgtga cagcgaggaa
     1921 gtggactatg ccacgggccc gaccggcaac gactcctcgt tcgacgtgca ggcgccctac
     1981 ctggtgaaac tgctgtccag cgcgccgctg tcggacaaca tcacctatta cttctacggc
     2041 atctttgccg aaaaaggcgg caacggcgaa gtcatcatcg aggacgcctg gttcagctac
     2101 gccgacatcg ccggcagcgg tgtcggcggc atgctcggcc agttccagat ttccgacctg
     2161 atgttcccgc gcgaagtgcg gctcaccttc caggactact acgtgtatcg atccgccggg
     2221 atcacctacg atcgcggcat catcctggac cgggccttcg gacccgtgga cctggccttc
     2281 ggcctcacca acggcaacgg catccaggaa aacgtcacca tcaacagtcc cggctacaag
     2341 cgcccggacc acatgttcga caacgattcc ggcaagagcc tgttcggccg catcggcacg
     2401 accgtcggtc cggtgaacgt cggcctgttc ggcctggtcg gcgagcagcg cagcgccgcg
     2461 ggcttcgccg gtgcggaccg gggcagccgc gacaccgaca agaccatcta cggcctggat
     2521 ttctccggcg cgttcaatgc ccgcacgcac tggtacgcgc agctgctgtg gaaccggtgg
     2581 gacgagttcc tggacgccga cccgaccgcc gactacgact ggttcggggg cttcgccggc
     2641 gtggacttca tcatgaacga ccgctgggcg ttgtcgctgc tctacaacta cgccgacgcc
     2701 agcgacttcg acggcaccgg caccgtgttc gagggcatcg acatcaattc cctgaccttc
     2761 ggcgcggcgt actatttcat gcgcaacgtg aaggccgtca tcgagctcaa cgtggacctg
     2821 ctgagcgagc agaccggcgg gccgcccttc gtgggccacc agtcgaagga gaactacctg
     2881 ctggtggggt tcgacgcggc gtactgaggg ctcccgcacc gccgacacgc cccgggggag
     2941 ggtatacccc ccccggggcg caggttcaga acgtcacccc cagcgtcagc accacccgcc
     3001 cgtcggccca cttgccgggt ccgaggaacg cctccacgtc cgagtcgtag cccgaggtgt
     3061 cgaagtacga caggtcggcg ctgaagttgc cccagctgcg gttgatgccc agctggtaat
     3121 cccagtaatc gctgccggcg gcacgcgaaa tgtcgttgaa gccggcggcg agcctgaaat
     3181 tgaagccggt ctcgccgagc ggcgcatccg cggcgagctg gtagaagaag gcgctttcac
     3241 cggtgttcac atagtcattg gcataggcca ccgtggcgct gtagcgctcg gcgaaaccga
     3301 gaatcgcagt gaactcgttg taatcgatgt cgaagccggg gttcgagccc gggtaggtgt
     3361 agcgggccac cgaaaggtcc agcgtcgcgt cgtcggtgag gccgaacaca tagccgacga
     3421 agaaatcgag ctccatgtcg atgccgtcgg gctggtcgaa gtcgacgttc gatgcccaga
     3481 tgccggcgta gaagccggcc ccgtagccga tgtcgaggcc ggcctgcacg gcgggcccct
     3541 cgtcggtctg ggagacgccc cggaaaacgt agtcgctggt ggcggtgagc gacccgccga
     3601 tccaccagcc gtcttcggcg tgcacggcgg cgggcgccgc cagcatcatg ccgcccgcca
     3661 gcgcggcggc gagcagcggg gtcctcgagt tgtacttggt catattgtcg atcctcttgt
     3721 catgcatggg gtggcacggg gcggcgagcg gcgctcagcg gccgttcttc ggccggatct
     3781 ccggaaactc cgggcggccg tcgtggcgga agtacgcacc gggcggccca taggcggcgg
     3841 ccggctccgg gaagcgatac agcagcagga tgtaggccgc cgccgagacg aagctggaga
     3901 cgaacaggcc ggtatccgcg ccgcccagca acgccgcccc gggggcgacg taccagccgg
     3961 tgttgatgaa cagcgagccg gccgcgacgc ccagcgccca ggccgtggtg gcctgccagt
     4021 tcagcccgcc cgcaaaccag taacgaccgc cgatctcgcc acggttgaac acctgcaggt
     4081 ggtcggcgta gtaatagccg cgccggctga tcagtccgac gatgttgacc accacccacg
     4141 gcgccccgag gatcaccagg atggcgagga acgaggtcac cagcagggcg acctgctcgt
     4201 agaacgcgcc gatgtacacc agcagcgtcg agatcaggcc caggccgatg gtgcactcga
     4261 cccggctgaa gcgcgtcacg atggccgaga agtccaggcc catgctgtag atgttgatca
     4321 ccgcctgcgc ggtgcccgcc accaggccga tgtacagcaa cggcagcagg taccaggtcg
     4381 gtgcgttgac caccaggccc agggcgtagt ccatggtggg atcggcgaac gacgccgagg
     4441 tgtaggcgcc ccacacgtag gcgccgccca tgccgatgaa gccgccgagc caggtgacca
     4501 ccatcagctg ccggtcgctg tagcgcttgc gggagacgtg ccgcgtccag tcgccggcgt
     4561 agggcccgta ggagttggtg gtcgctgcgc agacgatggc gcctgcgatc caggtcggcc
     4621 agaaggagcc gagcgcatag tcgccgggct ggtagctggc gtcgaaggtc gggtagtagg
     4681 cgaagatacc caccaccatc agcacgccgg cggtaggcac catcagcttg cagaacacca
     4741 ccatgttggc gtgccccagc acggcgatgc cgaccatcac gatcgacacc agcgcatagc
     4801 agagcagctg cagctcgagc tgcgaatcct cggtgccggt gacgcgcgcc accgagccgg
     4861 ccagtacgtc accggccgcc cagacacaca gggccgcgaa gacgatgcag gccgtcacgc
     4921 cgagcaacga accgatcagc cgccccttgg cgccgaagtg cgcaccgctg gccaccgggt
     4981 tgttggtgcc ggtgcgcggc ccgaacagcg acatcggcgc caacaggaag gaacccacgg
     5041 ccgtgcccac gacgatggcg ctgagcgcct cccaccagcc gaggccgagc gataccggaa
     5101 tccagccgat gatgatgatc ccgaaggtca tgctgccgcc gaacaggatc cagaagacgt
     5161 tgatcaactc cgatcgtcgc tggtcttccg ggatgtactc gacgccgacg ttctcgatcc
     5221 ggccgacgcg gtcgacgacc tcgtttacgc ggcctgtgtt ctggtgcggt tgctcgctca
     5281 tgagcctgtt cctccaactg gcgtttgttc tggtcttgtg tttcgtttcc agtcctttgg
     5341 aacggcgtcc ctgccgcagc cggcgggcga cagggcgacg gctcccccgg gccgtcgccc
     5401 tgcctgaatg ctctaccgat ggctccctgc gcgtcctcgg cctgtccgcc cccagcggtc
     5461 agacatgcag caggtactcg tcgcgctcgc gctgcggcac cggcaggttg gcctgctccc
     5521 actcctgccg cttggtgtcg acgtagtcgc gaatgaagcc gggcgcgaag acctcgtgga
     5581 ccagcgggtc ctgttcgaac gcttcgacgg cgtcgaccag cgaccgcggc aggttcgcga
     5641 tgttgcgatc cgcgtaagtg agcgtgtcgt tcggcggccc ggcatccagg ttctcccgga
     5701 tgccctccat ccccgctgcg agcatgaagg cggcgaccag gtaggggttc gccgccggat
     5761 ccacgctccg gttttcgatg cagggccggt tttcgggcca gcggatcata caggagcggt
     5821 tgttgtgacc gtagctgatc ttgatcggcg cccaggacac cgaaccgtcc atcagtcgcg
     5881 gcaccagccg gcggtaggag ttcaccagcg ggttggaaag cgccgtcagc gcgcgcgcgt
     5941 gcttgaggat cccggaggtg aaggcacggc actccgccgt gaaccgcttg ccgtcgcgca
     6001 gcgcgctctt ctgctccccc gtggcgtggt acaggcccat gttgaagtgg ttgcctgagc
     6061 cccatacggt ctcgtagggc ttgggcatat aggtcgcgca cagcccgaac tgcttggcca
     6121 cctggtgggt catcaggcgg aacagcacca tccggtcggc catctccagc accgacttgt
     6181 agtagaagtc gatttcgtac tggccctcgc cgccctccac gcccatgccg taggcctcga
     6241 gcccgatacc cggcatgtac tccaccaggc gctgcaggaa gtcggccgta tcgagcaagg
     6301 tctcgacgtc gtaggccggg cttggcttga cgtaggctga aggcgtgacc gggatgagat
     6361 ggctggcctc cttcccgagc gattcgggcc ggaacacgta gaactccggc tccacgccga
     6421 gcgtggccgt gtagccctgc tcacgggctt tctccacctg tcgcttcagg atcgagcgcg
     6481 ggcacagctc gaagggctcg cccttgtccg accacatgtc cgccgccatc caggcgaaac
     6541 gcgtgtccca gggcagcacg gtcaaagtgt cgaggtccgg cagcgaggtc acttcttctt
     6601 cgaccgggtt catggtgccg atgccgcaga tgccgcgcgg tgtgtagcgc gcgaagccgc
     6661 cgagcagctc cggcagccgc ttgatcggcg tcgccttggc gcgtgcccgc ccgagaatgt
     6721 ccacccagct tcccaacacg tagccgacgc cctgctcggc cagcgagcgg accacctcct
     6781 cgtggctggg gtcgaccggt ctaagcgtat ccatctcctc tcctttgctg ttttgctgca
     6841 cctgcagcag cgagcggccc ttgttctttg cctttcgcgg cgtgttgcgc gcgacgacgg
     6901 atcgaatata ctcacaactt atttagtcag tcaattaact ttttcgagga gacgccgcag
     6961 catgaaagtc gcaaacgcca cgccatcgga acgaacgcag caaacgccga acgaaacggc
     7021 cttccgcaac atgaccggcg cggagcccgt gctgctcgat gtggccccgg cgatcgatgt
     7081 catccccggc taccagcgca acgtcatcct cacgtccggg ccaccgctac cctgggacca
     7141 gtactacggc ggccagcgtg atgcggtgat cggcgcagcc ctctacgagg gtctcgcgcg
     7201 caacgcggag gaggcagacg cgctgatcgc tcgcggcggg attcgtctcg cgccttgcgg
     7261 cgagttcgac tgcgtgggct cgctggccgg cgtctactcg gcctccatgc cggtgttcgt
     7321 tgtgcaaaac aagacccacg gcaatttcgc ctactgcaac gtctacgagg gcagcaaccc
     7381 gcgacggctg aactacggcg tctacgacga gggcgtgcgc gcccggctga atcacatcgg
     7441 catggcggtg gcaccggtca tcggcgcagc ggtgcgagcg gccggcgaaa tacccctcaa
     7501 gcccatcatg aagcgcgcgc tgcacatggg cgacgagctg cacagccgca acaccgccgc
     7561 atcgctgctg ttcgggcgcg agctgttccc ctacctgctc gagcttcgcg aggagcgcgc
     7621 cgcccgcgtc gcagagacgg tgcgcgtgct caccgaggac cattacttct tcctgcgcct
     7681 gtccatggcg gcggccaaga ccatgggcga tgccgcccac ggcgtccccg gcagctcgct
     7741 ggtgagcgcc atggccttca actgccggca cttcggcatt cgcgtcagcg ggctgggcga
     7801 gcgctggttc ctcggcccgc acccggaggt gcatgcccgg ctgttcgacg gcaagaccga
     7861 ggccgacatc acctggatgg gcggcgagag caccatcacc gagacagtcg gtatcggcgg
     7921 tttcgcgcag gccgccgcct ttccactgca ggcctaccag ggcggatcac ccgaggcgat
     7981 ggtggacaac aacgtcagca tgtaccgcat cacccacgcg gagcacccgg acttccgcat
     8041 tcctttcctc gcctaccggg gcacgccgac cggcatcgac gtgctcaagg tgctggagac
     8101 cggcgttacg ccggtcatgg acctcggcat cgccggcaag ggcggcggcc agatcggcgc
     8161 cggcctggtg cgggcgcaca ttggctgctt cgaggcggcg gcgcgcgagt ttcgcgcaca
     8221 atacggcgag acctgatcag ctccggcccg cgccggatgc ggcgcgggcc cgggaccagg
     8281 cggcaggagg caggcacgcg gcatgctcag caacagctat ttctggcagt cgggcctggt
     8341 gttcctcatc ggcctggtgc cgttccttga gatctacgtg gcgatcccgg ccgggctcgc
     8401 catgggtctc gacccggtat ccagtttcgc ctgggcgacg ctgggaaact gcctgccact
     8461 cgcgctggtg ccgcgtttcc acgactgggt gcaccgccat gagcgcctcg gcggctggat
     8521 cgaccgcttc atcagcgaga aatgggagcg acgcttcaac cgctacggca agggcgcgct
     8581 gctggcgttc accccctggg tcggggtgtg ggccacggcg gccagcgccg ccgccctgcg
     8641 ggtcgccccg aacgtcatcg ttttctgggg ctgcctcagc atcgtgctgt acggtctcgg
     8701 cacggtgctg ctgggcgtcg gcgtgatgag tttctttggc ggcgactagc ggcccgtaat
     8761 ccggaggcgg acgatggact tgcagcgggt gctgacggaa gtcgccgaca tggccacgcc
     8821 gtatttcggc cgcggcgccc tgcccgacta catcccggcg ctggccgccg ttgaccctcg
     8881 ccagttcggc atggccgtaa tgacggtgag cggcgatgaa cacgccgtcg gcgacgtcga
     8941 cacgcccttc tcgatccaga gcatcagcaa gcttttgtcg ctgatcctgg cgatgaacct
     9001 ggagggcgag ctgttgtgga gccacgtggg ccgcgagccc tccggcacgc gtttcaactc
     9061 gctcgtgcag ctggagtacg agcgcggggt gccccgtaat cccttcatca acgccggcgc
     9121 gctggtaacg ctcgaccgcg tgctgccgcg ggtgcacgac taccgcacgc tgctgctcga
     9181 tttcgtgcgc tttttggcct gcagcaatgc catcgactac gatgcctcgg tggtcgcgtc
     9241 ggagcgcgac ggggcccatg tcaatttcgc gctggcgcac ctgctcaagt cctaccaccg
     9301 gatcgacagc ccggtggacg ccttgctgga cgcctacttc caccagtgcg cactggcaat
     9361 gagctgccga cagctggcac gcactttcct ggcgctggcc aatcgcgggc tgtcaccgtt
     9421 ggtcgaggag gcggtggtca cggcgcgcca ggcgaaacgc atcaactcgc tgatgctgac
     9481 ctgtggaatg tacgactccg tcggcagctt cgcgtttcgt gtcggcctgc ctgcgaaaag
     9541 cggagtcggc ggcggcatcg tcgccgtcgt ccccgatcgc atgtgcatcg ccacctgggc
     9601 gcccgagctc gacctgtcgg gcaactccta cgtaggcgtg ctcgcgctcg agcgcttcac
     9661 cgaaatcacc gggctgtcgg tattctgaac gtcagcgacg tgccacgcga ccggccgcgg
     9721 gctcgtagcc caggtgcgac cagagaaagt cctcgaggtc cttcaggctg cgcctgacgt
     9781 cgagcggacc gcgcgcgagg tgcgcggcca tgatgccgtc caggtggatc gacacgaagc
     9841 gggcggtccg ggccgcgttg accttgcgga acaagccgag gcggatcccc tcgcggatgc
     9901 tgtcggcaag tatgcccttc tcttcctggt ggtagaaatg cgcgatcagg tcgtcgatcg
     9961 agaactcgct gtcgaccatg ccgtgatagt cgatcatgat cttgatgacc tgcaggatgg
    10021 tctcggacat ctccgtgttg atgtggaacc agtgacggat ccgcgccacc gggtcccgat
    10081 cgccgtcgcg gatggtgcgg tactcgtcga gcaggcgacc gacggcgaat tcgagtgccg
    10141 agcggaacag gtcctccttg ctctcgaagt agtaatagat gaggcccgag ttcatccccg
    10201 ccgcggcgga gatctccttg ttcgtcacgg tcgcgaaatc acgccgcgca aacaggtcca
    10261 gcgccgcctc cagcagttgt tcgcggcgga cctccttgcc ggcgcgtttg cgcgtcccgg
    10321 ccggcccgac accctgcggc ttcgtcttcc tagccattcc agctcccttg cagcacggcg
    10381 catgccgggc gccgcacccc gcattctgac gccaagcggc ggcgctttcc agaagcgggg
    10441 ttcaggcgcg gaaatgccat ccaggtcaat ttccggtccc ggccagttga ccaggatcat
    10501 tccgggcccg gagccggcat gatctgggtc acgcccggtg cgaccggcct cgattgacgg
    10561 ccccgcgccc cgccactaga atttcgcgcc ttcacgggcg attagctcag cgggagagcg
    10621 ctcccttcac acgggagagg tcactggttc aatcccagta tcgcccacca atcccgcagc
    10681 gatgaaaacc ggggtcagag acgggtttct taggaaaccc gtctctgtcc ccggttttca
    10741 atgcccccgt tttctgaccc cggtttcccc gtattcagtc ctgctgcggt gcgttcatac
    10801 ttgcaggtgg cgtctgcccg cccaaatgga agggggaacg gggcatgtca caagcgatcg
    10861 acccgagcaa gtccgacgcc gccgccgcaa tcgacgaagt actggttgcg gagacggccg
    10921 tccgcgaggc catggaggcc tgccggcagg aggcggaacg cgtgctggat gccgcgcgcg
    10981 aggatgcgcg ccgcattgcg cgcatcgcca gccagcgcgc ttccaggctg catgcgcgct
    11041 gcgaccagct ggtggccggg caaatcgagg aaatccgccg ggcagccggc gtcgatgcgc
    11101 cgcgcaccga actcaatgcc gaggaccggg agcgactcgc cgcggcggtg gcgcgactcg
    11161 ccgcacgcct gacccggcca agcgatggct gacgacgccc gcctcgccta tctccaggcc
    11221 ggcctgcagg cccgcctcgg ggaccggccg gccgccgacg agcggcgcgt ggccgaggcc
    11281 agcatcgacc tgtcgcacta cctcgaggcg ctccgtcgca gctcgctgaa gcgctggatc
    11341 ggcgacctca acccggacat ggcccccgag gccgtcgagc gacagctgcg cgccgcctgg
    11401 cgcg
//
LOCUS       DAOMPG010000059        11240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2492_length_11240_cov_4.768619, whole genome shotgun sequence.
ACCESSION   DAOMPG010000059 DAOMPG010000000
VERSION     DAOMPG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_11240_cov_4.768619"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLS34_04775"
     CDS             <1..230
                     /locus_tag="PLS34_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015067601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86818.1"
                     /translation="GNPFGMTALSSGYMAMATVSDHGKDKEGEGKCGEGKCGGDKDKD
                     KEGEGKCGEGKCGGDKKEGKEGEGKCGDKRA"
     gene            295..858
                     /locus_tag="PLS34_04780"
     CDS             295..858
                     /locus_tag="PLS34_04780"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013786896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRP86819.1"
                     /translation="MGVSDAYLAYHDQEWGRPVRDDAGQFEFLVLESAQAGLAWSTVL
                     HKRDAYRRAFAGFDPEKVARFNRRSVERLVGDAGIIRNRLKIESAIGNARAFLAVRDE
                     FGSFSDYLWGFVDGRPVVNRWREMRQVPAATPLSDRISKDLKARGFKFVGSTIVYAHL
                     QATGLVNDHLVSCFRHAQCVAADRSSR"
     gene            complement(953..1570)
                     /locus_tag="PLS34_04785"
     CDS             complement(953..1570)
                     /locus_tag="PLS34_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86820.1"
                     /translation="MNRLQRTFWLAPILLLLAAHQAGHAAERAVSAPEKPPEEKSLLL
                     PAVQKAQAPEAESDAETKPARAVTPGPQKGIEPDEIDARNRARAPAAPDRAGATTRPA
                     PAAAPRASGQKLSVQQMMVKLKPRQPMAVAATTGGSTAGQNVQAKPAEPPKYEIADCG
                     TATSPMICCHHEAGDGSSCNLFKILCGNAGGTAQGDGESAACSDW"
     gene            complement(1595..2827)
                     /locus_tag="PLS34_04790"
     CDS             complement(1595..2827)
                     /locus_tag="PLS34_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86821.1"
                     /translation="MRFRWLAMMLGLMACAAADLSTAAILDVRATPRQASLVGTGANF
                     VAVRWAVATTPDHTSGVISTGADIIDPSSGAILQRVATTLNGSGGGPFVLRDALNLDA
                     ATVGAWVDAGLRRVVLRRSFADPATGASINGSVVLNLSASRLRATREAAPAGELAIIS
                     LQLEFDSGTNARIVGRAEPLQAVLTVQHTGSGTIRGRWQIAEPGSSEGVPVYRNLALV
                     NANVRAAQRSIYLSPALPTGRPGAYALRFCVLEAQEAGPGEDVMCPDADRIVTATYQV
                     QELEGEPAVVIRGLAPDRAVAAGNSVFRWQPVPAATLYQLQVFEPAAGDAQPANPPAG
                     QPQFVTGMLVRGPAAETRLTALVLSRLRPGQRYLWRITAHDESGRTVGASATATFVLD
                     PGASAPDSKNHNNSPEGH"
     gene            complement(2824..>3445)
                     /locus_tag="PLS34_04795"
     CDS             complement(2824..>3445)
                     /locus_tag="PLS34_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86822.1"
                     /translation="HSQPAADAVLAGFDVDSTTREFGIGLSLARDRLNWGLQIGAVHY
                     RDRSEAVFENGFLLYEPPSDSRTLQASVQAAWTLTERLSFDAYLQRNRLEETDVDNEY
                     RNTLYGAGTTILLLPERLSLRGGLSVGRDRNRFGDPWFLPEQLDSRFADLQLVWHAVA
                     ARPDRPGLSVNLSATYAHNEDRSLLVDDELWSVFLGARLDWKRSKR"
     assembly_gap    3446..3455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3456..4842)
                     /locus_tag="PLS34_04800"
     CDS             complement(<3456..4842)
                     /locus_tag="PLS34_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86823.1"
                     /translation="MRKLGWLLFAFGATAPTAAMQHPAAILADDPRSFSVELPASAFT
                     REGAAIAVDVNGYDLTPFGRLEGDILRVVLQTPLDPGQYALSVLLFLPDGGAEVLLDA
                     LLEVATPAGVQWSAGALLQTGYRLAEGPREAFDGVARDNAEGSLALAAGQAAGAWQLE
                     SALDVIYDRDSTATPGGEEWLLPNIRLAAAHRGELATTSLSAGNVVIAQENLLFSRYQ
                     RRGAHLASAAQSGRVELQAFSLVSAPSNLARSDQLLPTDSNNRSDGLAASLQLAEERL
                     ELSGAFVDGRTPLGGSGFNVLGDDAVYGGDSWNLQLRSQLADGSVLLQLEHAESRFDS
                     DGIGLGDPARKDDATSAILSLSSTGRFGSGPFSYWAAELQQRRVGLDFYSVGNLSLPG
                     NLEIRSAYLQAGYRNLAVDLDLGRERTNPGDDPALPTQTLDRRGVTVAYTPGTLDPDA
                     AVWQWLGAPSLN"
     gene            5065..5445
                     /locus_tag="PLS34_04805"
     CDS             5065..5445
                     /locus_tag="PLS34_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRP86824.1"
                     /translation="MISSLYGGLLGLLYLSLCWRVVDRRRQARVGLGSGGDAALERAI
                     RVHGNFAEYVPLFLLLLLAAELGGAATWLLHVLGVVFLVARIGHAYGLSRSSGTSRGR
                     FLGTLLTWVALLLAALLNLWLALG"
     gene            complement(5461..5892)
                     /locus_tag="PLS34_04810"
     CDS             complement(5461..5892)
                     /locus_tag="PLS34_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP86825.1"
                     /translation="MMRPRFHLAFPVHDLEAARGFYAGLLGCGVGRESRRWIDFDFHG
                     HQITAHLVDDDRPSAANPVDGDRVPARHFGLILGRDEWDALAARLTAAGVAFLIEPRV
                     RFRGRPGEQATMFVTDPSGNALEFKSFADEAAIFAPDPGVG"
     gene            complement(5889..6815)
                     /gene="hemF"
                     /locus_tag="PLS34_04815"
     CDS             complement(5889..6815)
                     /gene="hemF"
                     /locus_tag="PLS34_04815"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRP86826.1"
                     /translation="MQHSVGQKEIEAVESWLKSLQDQLTAEIEALDGGARFRSDSWQR
                     AEGGGGDSRVIEEGALLEKGGINFSSVHGAALPPSATARRPELAGRSFRATGVSMVLH
                     PRNPYVPTTHGNWRFFVAEKPGEAPVWWFGGGFDLTPYYPYLEDVQHWHRQSRAACLP
                     FGEDVYPKYKRWADEYFHLKHRNEPRGVGGLFYDDLNDWGFARCFEFSRTVAAHFLPA
                     YTPIAARRRHTPWGEREREFQLYRRGRYVEFNLVYDRGTVFGLQSGGRIESILMSLPP
                     LVRWRYDWQPEEGSPEAALYHEFLRPRDWLEE"
     gene            complement(6842..7402)
                     /locus_tag="PLS34_04820"
     CDS             complement(6842..7402)
                     /locus_tag="PLS34_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRP86827.1"
                     /translation="MAGSLAIRRAGALIRAGGVVAYPTEAVWGFGCDPLDAEAVARIL
                     AVKQRPASMGLILIAADEAQLRPWVEPVSAEIEARVGAAWPGPVTWVMPAQPWVPDWV
                     HGGRGSVAVRVTAHAQSAALCRTTGFAIVSTSANRSGKPPARSSIQVRRWFGDEIDLV
                     LGGATGGRDRPSEIRDAVSGRPIRAA"
     gene            complement(7405..9918)
                     /locus_tag="PLS34_04825"
     CDS             complement(7405..9918)
                     /locus_tag="PLS34_04825"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HRP86828.1"
                     /translation="MSKNLVIVESPAKAKTIEKYLGGDFKVLASYGHVRDLVAKEGAV
                     DPDAGFAMKYQEIERNKRHLEAIARALRGANALYLATDPDREGEAISWHVCEALRERG
                     LLDGRPVYRIVFHEITRRAVQEALEHPREVSGDLVNAQQARRALDHLVGFNLSPLLWK
                     KVRPGLSAGRVQTPALRLIVEREAEIEAFKPVEYWTLEAELEKSGQAFPARLAEYGGE
                     RIDDKLEKFFIGNEGAAREAEKTLREAAAGKLQVTSVERRQRRQNPAPPFTTSTLQQE
                     AARKLGFTAQKTMRTAQRLYEGVDFGEGATGLITYMRTDSVSLAADALNEIRDVIGQR
                     FGARALPDKPRFYRTKAKNAQEAHEAIRPTSAGWHPDDLAGRLDRDQQRLYELIWKRA
                     VASQMASAVFDTVSLDLVPSGAPAAGHRFRASGSILVEPGYIAVYQEGRDDAEDEQDR
                     RLPQVKEGELIQLNKLEAEQHFTKPPPRFSEASLVKTLEEFGIGRPSTYATIISTLVD
                     REYVELDRKRFIPTDVGRIVSNFLSGHFTRYVDYDFTARMEDRLDAISRGEQDWQELL
                     GRFWEPFQELIKEKESSVSKRDVMPNRELGKDPASGKPVLVKIGRFGPYVQIGTAEDE
                     DKPRFAGIPRSKSMQTITLEEAMELFKLPRELGATPDGKPVLVNIGRFGPFVKYGSRY
                     ASLKKDDDPYTIDLERALEIVRDKEAAEAARTLREWPEHDIRILKGRFGPYVTDPGRR
                     ASIPKGKDPAAVTLEEAIRLLEEAPAKKGARGKKKATSKKAASKKAPAKKAAAKKKTT
                     TRKKAPARKKPAARKKAAGAKQAAAKKTAARKAAGDRGA"
     gene            complement(10010..10486)
                     /locus_tag="PLS34_04830"
     CDS             complement(10010..10486)
                     /locus_tag="PLS34_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HRP86829.1"
                     /translation="MTESVLDVLMYLFETAGDEGGPPEPNRAGLREELERAGFHDREI
                     DRALDWLDGLATRRADEGGEPPTERAIRIYGRDECVRMDARGRGYMMYLEQIGILSAH
                     QRELVIDRLMALDCQEIDVDDIKWVVLMVLFSQPGEETAYARMEDLVFEDRAWVAH"
     gene            complement(10488..>11240)
                     /gene="dprA"
                     /locus_tag="PLS34_04835"
     CDS             complement(10488..>11240)
                     /gene="dprA"
                     /locus_tag="PLS34_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRP86830.1"
                     /translation="NPTPQGAENARAFARYLAGCGLVICSGLAAGIDAAAHRGALDAG
                     APTTAVLGCGPDRIYPAENAPLARQVAAAGLILSEFLPGTPPKKEHFPQRNRIISGLS
                     LGVLVVEAAKRSGSLITARLAGDQGREVFAIPGSIHNPLARGCHALIRQGAVLVETAH
                     DILAEIGPLAGVSMQSEATDVPPPELAPEPDADYRKLLAALAHDPCGVDELAARTGLT
                     AAEVSSMMLILELQGAVESLPGARFVRKTTRS"
BASE COUNT         1599 a         3848 c         3943 g         1840 t
ORIGIN      
        1 acggcaatcc cttcggcatg acggcgctgt ccagcggcta catggccatg gcgaccgtca
       61 gcgaccacgg caaggacaag gaaggcgaag gcaagtgcgg tgagggcaag tgcggcggtg
      121 acaaggacaa ggacaaggaa ggcgaaggca aatgcggtga gggcaagtgc ggcggcgaca
      181 agaaggaggg caaggagggc gaaggcaagt gcggtgacaa gcgcgcctga tcgccactcg
      241 ccgggcggtg gggctcgcgg tgcggaccgc gggctccgcc gctgcgactg gtgcctgggg
      301 gtcagcgatg cttaccttgc ctatcacgac caggaatggg gacggccggt gcgcgacgac
      361 gccggccagt tcgagttcct ggtgctggag agcgcccagg ccggcctggc ctggtcgacg
      421 gtgctgcaca agcgggacgc ctaccgccgg gcattcgcgg gcttcgaccc ggagaaggtc
      481 gcgcgcttca accgccgcag cgtagagcgc ctggtgggcg acgccggcat catccgcaac
      541 cggctgaaaa tcgagtcggc gatcggcaac gcgcgcgcct ttctcgcggt gcgcgacgag
      601 ttcggcagct tcagcgacta cctctggggc ttcgtcgacg gccggccggt ggtcaatcgc
      661 tggcgcgaga tgcgccaggt gccggccgcg acgccgctgt ccgaccgcat cagcaaggac
      721 ctcaaggcac gcggcttcaa gttcgtgggc agcaccatcg tgtatgccca cctgcaggcg
      781 accggtctgg tgaacgacca cctggtgagc tgcttccgcc acgcgcaatg tgtcgcggcg
      841 gaccgcagct cacgctagcc gccacgcccc ccccgcaaag aaaaccggcc accccgcagg
      901 gtggccgcaa agtcgacatg gcgggccttc catggaggcc cgcaagcgat gattaccagt
      961 cggagcaggc cgccgattcg ccgtcgccct gcgccgtgcc gccggcattc ccgcagagaa
     1021 tcttgaacag gttgcagctc gagccgtcac ccgcctcgtg atggcagcag atcatcgggc
     1081 tcgtagcggt gccgcaatcg gcgatctcgt acttgggcgg ctcggccggc ttcgcctgga
     1141 cgttctggcc ggcggtgctg ccgccggtcg tcgcggccac cgccatcggc tgccgcggct
     1201 tcaacttcac catcatctgt tgcacggaca gtttctggcc gctcgcccgg ggcgctgcgg
     1261 caggtgccgg gcgggtcgtc gccccggcac ggtccggtgc ggcgggtgca cgggcgcgat
     1321 tcctggcgtc gatctcgtcc ggctcgatgc ccttctgcgg ccccggcgtc accgcgcggg
     1381 ccggcttggt ctcggcatcc gattccgcct cgggcgcctg ggccttctgt acggcgggca
     1441 gcagcagcga cttctcctcg ggcggcttct ccggtgcgct cacggcgcgc tccgctgcat
     1501 gtcccgcctg atgggcggcg agcaacagca ggatgggggc gagccaaaac gttctctgca
     1561 gtctgttcat ggggactctc tccagtgggt tgggtcaatg cccttcgggg ctgttgttgt
     1621 ggtttttcga atcgggcgcc gaagcgcccg ggtccagcac gaaagtggcc gtggcgctgg
     1681 cgccgacggt gcggcccgat tcgtcatgcg cggtaatccg ccacaggtat ctctggccgg
     1741 ggcgcaaccg gctgagcacg agcgccgtca accgggtttc agctgccggg ccgcgcacca
     1801 gcattccggt gacgaactgc ggttgcccgg cgggcggatt ggccggctgc gcgtcgccgg
     1861 ccgcaggctc gaacacctgc agctggtaca gcgtggccgc ggggaccggc tgccagcgga
     1921 acacgctgtt ccccgcggcg acggcgcgat ccggcgcgag gccccggatg accacggcgg
     1981 gctcgccctc gagctcctgc acctggtagg tcgcggtgac gatccggtcc gcgtccgggc
     2041 acatcacgtc ctcgcccggg ccggcctcct gcgcctccag cacgcagaac cgcagcgcat
     2101 aggcgcccgg gcgcccggtg ggcagcgcgg ggctcaggta gatggagcgc tgtgcggcgc
     2161 gtacgttggc gttgaccagc gcgaggttgc gatagaccgg cacgccctcg gagctgccgg
     2221 gctcggcgat ctgccagcgc cccctgatgg tcccgctgcc cgtgtgctgc accgtcagca
     2281 cggcctgcag cggctcggcg cggccgacga tacgggcatt ggtgccgctg tcgaactcca
     2341 gctgcagcga gatgatcgca agttcgcccg ccggggccgc ctcgcgggtt gcgcgcaggc
     2401 ggctggcgct gaggttcagc accaccgagc cgttgatgga ggcgccggtg gccgggtcgg
     2461 cgaagctgcg ccgcaacacc acccgccgca ggccggcgtc cacccaggca ccgacggtgg
     2521 cggcatcgag attcagggcg tcccgcagca cgaagggacc gccgccgctg ccgttcagcg
     2581 tggtggccac gcgctggaga atcgcgccgg aggacgggtc gatgatgtcg gcgcccgtcg
     2641 agatgacccc ggaggtgtgg tccggggtgg tggccaccgc ccacctgacg gccacgaagt
     2701 tggcgccggt gccgaccagg ctcgcctggc ggggcgtcgc cctgacgtcg agaatcgccg
     2761 cagtggagag atccgccgcg gcacaggcca tcaggccgag catcatcgcc agccatcgaa
     2821 atctcatcgc ttgctccgct tccagtcgag acgcgccccg agaaacaccg accacagttc
     2881 gtcgtccacc agcaggctcc ggtcctcgtt gtgcgcgtag gtggcgctca ggttgacgct
     2941 caagcccggg cggtccggtc gggcggccac cgcgtgccat acgagctgca ggtcggcaaa
     3001 gcgggaatcc agttgctccg gcaggaacca cgggtcgccg aaacgattgc gatcccgccc
     3061 gacgctcagc ccgccgcgca ggctgagtcg ctccggcagc agcaggatcg tcgtgcccgc
     3121 gccgtacagc gtgttgcgat attcattgtc gacgtcggtc tcctcgagcc tgttgcgttg
     3181 caggtaggcg tcgaagctca ggcgttcggt cagcgtccag gcggcctgca ccgaggcctg
     3241 cagcgtgcgg ctgtcggacg gcggttcgta gagcaggaag ccgttctcga acaccgcttc
     3301 gctgcggtcc cggtagtgga cggcccctat ttgcaggccc cagttgagcc ggtccctggc
     3361 caacgacagg ccgatcccga attcccgggt ggtgctgtcc acgtcgaagc cggccagcac
     3421 ggcatcggct gccggctggc tgtggnnnnn nnnnncgttc agcgacgggg cgccgagcca
     3481 ttgccacacg gctgcatcgg gatcgagggt tcccggcgtg taggcaacgg tcacgcccct
     3541 gcggtccagc gtctgggtcg gcagcgccgg atcatcgccg gggttggtcc gttcacggcc
     3601 aaggtcgaga tcgacggcga gattgcgata gcctgcctgc aggtaggcgc tgcggatctc
     3661 cagattgccg ggcaggctga gattgccgac gctgtagaag tcgagcccga cgcgccgctg
     3721 ctgcagctcg gccgcccagt aggagaacgg gccgctgccg aagcggccgg tggacgacaa
     3781 cgagaggatt gcgctggtcg cgtcgtcctt gcgcgccgga tcgccgagcc cgatgccgtc
     3841 ggagtcgaag cgtgattcgg cgtgctccag ctgcagcagg acgctgccgt cggccagttg
     3901 cgagcgcagc tgcaggttcc agctgtcgcc gccgtagacg gcgtcgtcgc ccagcacgtt
     3961 gaagccggag ccgcccagcg gagtgcggcc gtccacgaat gctcccgaca gctcgagtcg
     4021 ctcctctgcg agctgcagtg acgccgccag gccgtcgctg cgattgttgc tgtcggtcgg
     4081 cagcagctgg tcgctcctgg cgaggttgct cggggcggag accaggctga aggcctgcaa
     4141 ctccacgcgt cccgactgag cggcggatgc gaggtgcgca ccacgccgct ggtagcgcga
     4201 gaacagcagg ttctcctgcg cgatgacgac gttgccggcc gaaagcgagg tggtggcgag
     4261 ctcgccccgg tgggcggccg ccagcctgat gttcggcagc agccactcct cgccacccgg
     4321 cgtcgccgtg ctgtcacggt catagatcac gtcgagtgcg gactccagct gccatgcgcc
     4381 ggcggcctgc ccggcggcaa gggcgaggct gccctcggcg ttgtcgcgcg cgacgccgtc
     4441 gaatgcctcc cgcggtcctt cggcgagccg gtagccggtc tgcagcagcg cacccgccga
     4501 ccattgcacg cccgccggcg ttgcaacctc gagcagggcg tcgagcagca cttccgcgcc
     4561 gccgtccggc aggaacaaca gcacgctcaa ggcgtactgt ccgggatcca gcggcgtctg
     4621 cagcaccacc ctgagaatgt cgccctcgag ccggccgaac ggcgtgagat cgtagccgtt
     4681 cacgtcgacc gcgatggcgg cgccctcgcg cgtgaacgct gaagcaggga gctcgacgct
     4741 gaagctgcga ggatcgtccg cgagaattgc cgcgggatgc tgcattgctg cggtcggtgc
     4801 tgttgcgccg aaggcgaaca gcagccagcc aagtttcctc atgccgatcc tccggcaagc
     4861 tggactcagg ctttgtacac gtttctgcaa cagaaagcgt gaagtgatcg gcaaaacccc
     4921 atcgtttgtt gcaccgcatg ggtcgccaca aacggtccgg cgcagctcgt tcgcccggcc
     4981 ggggcggggc gggcacgccc gacgccatgt cgttcaggta agattggcgc ccccgtcagc
     5041 gcgaggaatt ccgtgttgag tcccgtgatc agcagcttgt atggcggcct gctgggcctc
     5101 ctctaccttt ccctgtgctg gcgggtcgtg gaccgccggc ggcaggcccg ggtcggcctc
     5161 gggagcggcg gtgatgcggc actcgagcgc gcgatccgcg tgcacggcaa cttcgccgag
     5221 tacgtgccgc tgttcctcct gctcctgctg gcggcggagc tgggcggcgc cgcgacctgg
     5281 ctgctgcacg tgctgggggt ggtcttcctg gtcgcccgca tcggccatgc ctatggcctg
     5341 agccgcagca gcggcacctc gcggggacga ttcctcggca cgctgctgac ctgggtcgcc
     5401 ctgttgctgg cggcgctgct caacctgtgg ctggcgctgg gctgaggccg cgggacgggc
     5461 tcagccgacg ccgggatccg gcgcgaagat cgcggcttcg tcggcgaagg acttgaactc
     5521 cagtgcattg cccgaggggt cggtgacgaa catcgtcgcc tgttcacccg gccggccgcg
     5581 gaagcgcacg cgcggttcga tcaggaacgc cacgccggcc gccgtgagcc tggcagccag
     5641 cgcatcccat tcgtcgcgtc cgaggatcag cccgaaatgc cgcgccggca cccggtcgcc
     5701 gtccacgggg ttggcggcgc tgggccggtc gtcgtccacg aggtgggcgg tgatctggtg
     5761 gccgtggaag tcgaagtcga tccagcggcg cgactcgcgc ccgacgccgc agcccagcag
     5821 cccggcgtag aagccgcgcg ccgcctcgag gtcgtgcacc gggaaggcga ggtggaagcg
     5881 gggccgcatc attcttccag ccagtcgcgg ggcctgagga actcgtgata cagggccgcc
     5941 tcggggctgc cttcctccgg ctgccagtcg tagcgccagc gcaccagcgg cggcagcgac
     6001 atgaggatgg actcgatgcg gccgccggat tgcagcccga acaccgtgcc gcggtcgtag
     6061 accaggttga actcgacgta gcggccgcgc cggtagagct ggaactcgcg ctcgcgctcg
     6121 ccccacggcg tgtgccggcg cctggccgcg atgggcgtgt aggccggcag gaaatgtgcc
     6181 gcgacggtgc gcgaaaactc gaaacagcgt gcgaagcccc aatcgttgag gtcgtcgtag
     6241 aacaggccgc cgacgccgcg cggctcgttg cgatgcttga gatggaagta ctcgtcggcc
     6301 cagcgcttgt acttcggata gacgtcttcg ccgaacggca ggcaggcggc gcgtgactgg
     6361 cgatgccagt gctgcacgtc ctcgaggtag ggatagtagg gcgtcaggtc gaagccgccg
     6421 ccgaaccacc acaccggcgc ctcgccgggc ttttccgcga cgaagaagcg ccagttgccg
     6481 tgcgtcgtgg gcacgtaggg attgcgggga tgcagcacca tggaaacgcc ggtggcgcgg
     6541 aaactgcggc cggccagctc gggccgtcgc gccgtcgccg agggcggcag cgcggcaccg
     6601 tgcacggacg agaaattgat gccgcccttt tcgagcaatg ccccttcttc gatcacgcgg
     6661 ctgtcgccgc cgccgccttc cgcgcgctgc cagctgtcgc tgcggaaacg ggcgccgccg
     6721 tcgagcgcct cgatctccgc cgtcagctgg tcctgcaagg acttgagcca gctctcgact
     6781 gcctcgattt ccttctgtcc cacggaatgc tgcatggtta ctccggtcac gcggcgggtc
     6841 gtcaggcggc ccggatcggt cggccgctga cggcgtcgcg aatctcgctg gggcggtcgc
     6901 gaccaccagt ggcgccgccg agcaccaggt cgatttcgtc gccgaaccag cggcgcacct
     6961 ggatcgacga gcgggccgga ggtttgccgc tgcggttggc gctggtcgaa acgatggcga
     7021 agccagtcgt ccggcacagc gcagccgatt gcgcgtgcgc cgtgacgcgc acggcgacgg
     7081 agccgcgccc gccgtgcacc cagtccggca cccacggctg cgcgggcatc acccaggtca
     7141 ccgggccggg ccaggcggcg ccgacgcggg cctcgatttc cgcggacacc ggctcgaccc
     7201 atgggcgcag ctgcgcctca tcggccgcga tcaggatcag tcccatggac gccgggcgct
     7261 gcttgacggc gaggattcgc gcgacggctt ccgcatccag gggatcgcag ccgaatcccc
     7321 agacggcctc ggtgggatag gcgaccacgc cacccgcccg aatcagggcc ccggcccggc
     7381 ggatcgcgag gctgccggcc atgatcaggc gccgcggtcc ccggcggcct tccttgcggc
     7441 agtcttcttg gcggcggcct gtttcgcgcc ggccgccttt ttcctggccg ccggcttctt
     7501 cctggcgggc gccttcttcc tggtggtcgt cttcttcttc gcggcggcct tctttgccgg
     7561 ggcctttttc gaggcggcct tcttgcttgt cgcttttttc ttgccgcgcg cgcccttctt
     7621 cgccggcgcc tcctcgagca gcctgatggc ttcctcaagg gtcaccgcgg cggggtcctt
     7681 gcccttcggg atgctcgcgc gccggcccgg gtcggtcacg taaggaccga agcgcccctt
     7741 caggatgcgg atgtcgtgct ccggccactc gcgcagcgtg cgggcggcct cggccgcttc
     7801 cttgtcacgg acgatttcca gggcgcgctc cagatcgata gtgtagggat cgtcgtcctt
     7861 cttcagcgag gcgtaccggg agccgtactt cacgaagggg ccgaagcggc cgatgttcac
     7921 cagcaccggc ttgccgtcgg gcgtcgcgcc gagctcgcgt ggcagcttga acagttccat
     7981 cgcttcctcg agcgtgatgg tctgcatgct cttgctgcgc gggatgcccg cgaagcgcgg
     8041 cttgtcctcg tcctcggcgg tgccgatctg cacgtagggc ccgaaacgac cgatcttgac
     8101 cagcaccggc ttgccgctgg cggggtcctt gccgagctcg cggttcggca tgacgtcgcg
     8161 cttgctgacc gagctctcct tctccttgat caactcctgg aagggctccc agaagcgtcc
     8221 cagcagttcc tgccagtcct gctcgccgcg cgagatggcg tcgagccggt cctccatgcg
     8281 cgccgtgaag tcgtaatcga cgtagcgcgt aaagtgcccg ctgaggaaat tgctgacgat
     8341 ccggccgacg tcggtcggga tgaagcgctt gcgatcgagt tccacgtact cgcgatcgac
     8401 cagcgtcgag atgatggttg catacgtcga gggccggccg atgccgaact cctcgagtgt
     8461 cttgaccagg ctcgcctcgc tgaaacgcgg cggcggcttg gtgaagtgct gctcggcctc
     8521 gagcttgttg agctggatga gttcgccttc cttgacctgc ggcagccggc gatcctgctc
     8581 gtcttcggcg tcatcgcggc cttcctggta gacggcgatg tagccgggtt ccacgaggat
     8641 cgagccggag gcgcggaaac ggtgcccggc cgcgggcgcc ccggacggca ccaggtccag
     8701 cgacacggtg tcgaacaccg cgctggccat ctggctggcg acggcacgct tccagatcag
     8761 ttcgtacagg cgctgctggt cgcggtccag ccgtcccgcg aggtcgtccg gatgccagcc
     8821 ggcggaagtc ggccggatcg cctcgtgggc ttcctgtgca ttcttcgcct tggtgcggta
     8881 gaagcgtggc ttgtccggca gggccctggc gccgaagcgc tggccgatga cgtcgcggat
     8941 ctcgttgagc gcatcggcgg ccagcgacac cgagtcggta cgcatgtagg tgatcaggcc
     9001 ggtggcgccc tcgccgaaat ccacgccttc gtacaggcgc tgcgcggtgc gcatggtctt
     9061 ctgggcggtg aagccgagct tgcgcgcagc ttcctgttgc agggtcgagg tggtgaacgg
     9121 cggcgccggg ttctggcggc gctggcggcg ctcgaccgaa gtgacctgca acttgccggc
     9181 cgcggcctcg cgcagcgtct tctctgcctc gcgcgcggcg ccctcgttgc cgatgaagaa
     9241 cttttccagc ttgtcgtcga tgcgctcgcc gccgtattcg gcgagccgcg ccgggaacgc
     9301 ctggccggat ttctccagct cggcctcgag cgtccagtac tcgaccggct tgaaggcctc
     9361 gatttcggcc tcgcgctcca cgatcagccg cagcgccggg gtctgcacgc ggccggccga
     9421 caagcccggc cggaccttct tccacagcag cggtgagagg ttgaagccca ccaggtgatc
     9481 cagcgcgcgg cgtgcctgct gtgcattcac caggtcgccc gatacctcgc gcgggtgctc
     9541 gagcgcttcc tgcacggcgc gcctggtgat ctcgtggaac acgatgcgat acaccggccg
     9601 gccatcgagc aggccgcgtt cccgcaaggc ctcgcagacg tgccagctga tggcttcgcc
     9661 ctcgcggtcg gggtcggtcg cgaggtagag ggcgttcgcg ccacgcagcg ccctggcgat
     9721 ggcttcgagg tggcgcttgt tgcgctcgat ctcctggtac ttcatcgcga agccggcatc
     9781 cgggtccacg gccccctctt tcgcgaccag gtcgcgaacg tggccgtagg aggcgagcac
     9841 cttgaagtcg ccgccgagat atttctcgat ggtcttggcc ttggccgggg actccacgat
     9901 gactaggttc ttgctcattt cacactggtt cagctggtcg aaaaaacggg ccgtggctcg
     9961 cgccacgacc cctgtgtcgg catgtccggg ccgcgcggca ggccgggccc tagtgtgcta
    10021 cccaggcccg gtcctcgaac acgagatctt ccatcctcgc gtaggcggtc tcctcgccgg
    10081 gctggctgaa cagcaccatg aggaccaccc acttgatgtc gtcgacatcg atctcctggc
    10141 agtcgagggc catcaggcgg tcgatcacca gctcgcgctg gtgtgccgag agaatcccga
    10201 tctgctccag gtacatcatg tagccccggc cgcgggcgtc catgcgcacg cactcgtcgc
    10261 ggccgtaaat gcggatggcg cgttcggtgg gcggctcgcc accctcgtcg gcgcgccgcg
    10321 tcgccaggcc gtcgagccag tccagcgccc ggtcgatctc acgatcgtgg aagccggcgc
    10381 gctcgagttc ttcccgcagc ccggcgcggt tgggctccgg tggcccgccc tcgtcgccgg
    10441 cggtctcgaa caagtacatc agaacgtcga gcacactctc cgtcatctca ggacctcgtc
    10501 gttttccgga caaagcgtgc gcccggcaag gactccactg ctccctggag ttcaaggatc
    10561 agcatcatcg aggaaacctc cgcagccgtc aaaccggtgc gcgccgccag ttcatcgacc
    10621 ccgcaggggt cgtgcgcgag tgcggccagc agcttccggt agtccgcgtc gggctccggc
    10681 gcaagctccg gcggcggcac atcggtcgcc tcggactgca tcgacacgcc tgccaggggg
    10741 ccgatctcgg ccaggatgtc gtgcgccgtc tcgaccagca cggcgccctg gcgaatgagc
    10801 gcgtggcagc cgcgggccag cggattgtgg atgctgcccg ggatggcgaa cacttcgcgg
    10861 ccctggtcgc cggccagccg ggccgtgatc agcgagccgc tgcgcttcgc cgcctcgacc
    10921 accagcaccc cgagcgagag cccgctgatg atgcggttgc gctgcgggaa gtgctccttt
    10981 ttcggcggcg tgcccggcag gaactccgac aggatcaggc ccgccgcggc gacctgccgg
    11041 gccagcggcg cattctcggc cggatagatg cggtccggcc cgcaaccgag cacggcggtg
    11101 gtcggcgccc ctgcgtcgag cgcgccgcga tgcgccgccg cgtcgatgcc ggccgccagc
    11161 ccgctgcaga tcaccaggcc gcagccggcc aggtaccggg cgaacgcgcg cgcgttctct
    11221 gcaccctgcg gtgtcgggtt
//
LOCUS       DAOMPG010000060        11150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2509_length_11150_cov_4.387562, whole genome shotgun sequence.
ACCESSION   DAOMPG010000060 DAOMPG010000000
VERSION     DAOMPG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_11150_cov_4.387562"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLS34_04840"
     CDS             complement(<1..424)
                     /locus_tag="PLS34_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRP86831.1"
                     /translation="MAELFFHNARVYPKADGDEVLDCFAVEQGRIVSLGEAVSAARAV
                     DLGGRLVMPGLIDCHTHLVWAGDRADEFAMRQAGAGYAGIARAGGGILSTVEAVRAAD
                     EDELVALALPRLAALAAEGVTCVEIKSGYGLDLDSELKM"
     gene            456..>1660
                     /locus_tag="PLS34_04845"
     CDS             456..>1660
                     /locus_tag="PLS34_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86832.1"
                     /translation="MSRELNFEHILTPAGIERHRSLRIDGHGRISAIEPCAPGGPFDG
                     WLALPGMPSAHSHAFQRGLAGHGEAARGADSFWSWREAMYRLAAGLDADALYALSFQA
                     YREMLAAGFTSVAEFHYLHRRADGERATELIEAVIRAGSDAGIRLAFIPVFYQRAGFD
                     TPAGPGQARFLHRDVEDFLTLVAMYAEHCAGVAAHSLRAVPPEMLADMALGAGELLGA
                     DRPIHIHIAEQPAEVEACLAATGRRPVQLLADTVELGPQWSLVHATHADAAERARIIA
                     GGATVVLCPLTEAYLGDGLFPAAEFAAAGGRLAIGSDSNVRIDAIEELRWLEYGQRLL
                     TGRRGCFADAAGLGGALWRRLAAGVPRQTRANRKAPNFRRERVSARIRRQGFPDYFGK
                     FRPVQAPNS"
     assembly_gap    1661..1670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1815..2639
                     /locus_tag="PLS34_04850"
     CDS             1815..2639
                     /locus_tag="PLS34_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRP86833.1"
                     /translation="MTPPGRTPTQPDVAPFGERAPTAPPVPLVVSIPHTGTWLPPEVR
                     DSLASPAMLAQPMTDWHLHLLYDFLPALGATTIFATVSRFAVDLNRPPQPRALYPGRF
                     ETGLVPLETFQGEAVFRSPPDADEVERRRRAWHAPYHARLQALLDETRARFGRVVLVD
                     AHSVASAPNRLHGALEDEIYLGDRDGATCGGWLRELLRDGFVAQGLSVALNAPYKGGY
                     ITDHYGRQAGVEAIQIEMAQRVYMDESDPAGGPEHPRFAAAQQRLLRVLGQLADKL"
     gene            complement(2659..3468)
                     /locus_tag="PLS34_04855"
     CDS             complement(2659..3468)
                     /locus_tag="PLS34_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRP86834.1"
                     /translation="MKSWIIPTALLAATLMAAPAGAHDHDALAKAIAGSHRAEANVAR
                     DGARHPGETLEFFGFAQGQTVVEVSPGGGWYTEILAPVLRGHGKLVAAHFPEDSDSAY
                     MQRVRGEFLAMLAAAPAVYDEVEVIGYKTGSDDALGAPGSADLVLTFRNLHGLYRQGN
                     LEAFIADAFAVLKSGGTLGVVQHRAPAGSDPEAGKDSGYLPQDWVVAQFEAGGFKLAG
                     SAEINANAKDTADHEGGVWALPPTLRHGDKDREKYLAIGESDRMTLRFTKP"
     gene            3585..4325
                     /locus_tag="PLS34_04860"
     CDS             3585..4325
                     /locus_tag="PLS34_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP86835.1"
                     /translation="MAIETFPVRLEWRRMATPHVLHLAFRKSDDGTLEYAPGQFLNLH
                     FEAASGGTHRSYSIANPPGPDGLLEIAMSPVAGGLATEALSTLAPGGELQASGPFGRF
                     VLREEPPCRYVLVGTGTGITPYRAMLPLLGERLKAGFRAHVVLGVWRREELLFGADFI
                     AAARTLPGFEFTGCYSRELPADPEPWERPGYVQTTYSSLDLDPEKDVVYLCGNPGMID
                     ESVEILKAMGFTLKQLRREKYLSARPAR"
     gene            complement(4441..7122)
                     /locus_tag="PLS34_04865"
     CDS             complement(4441..7122)
                     /locus_tag="PLS34_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRP86836.1"
                     /translation="MKLSSLAVSLSAAVATAMSVAPLSHADDLAVADVSQVSIEHDVS
                     PTRWFVEFAGPPTVRGVSPGAVQREHDAFRRAAGAAGVELRERFRYQTLWNGVSVETS
                     ASGAATLRSIPGVKAVYPVVEFDAPPAPDSGSVGDVASAIAQTRVDIARSELGLTGAG
                     IKVAIVDTGIDYDHPDLGGCFGPGCKVAYGFDFVGDAYNAGDPANNLPIPDDDPDDCN
                     GHGTHVAGIVGASAAAEDGVTGVAPDVTLGAYKVFGCGGSSSADVIVAALERAYADGM
                     QVINQSLGAAFQWPSYPTATVGDRLVEQGVVVIASAGNQGATGTFSIGAPGVGRDVIG
                     VASFDNARVRAPYFEAGGNRIGYQTMTFSSAAPTSGSEEIVYIGQACNTDALLADPTG
                     KAALVVRGVCGFAEKAVNAVNAGATAVVVYNNVAGIVAGTLGAPQGFSQPVVGISLAD
                     GLFLRGLAAPATITWTDGIDRFPNPTGDLISAFSSWGMSPDLQIKPDLGAPGGAIYST
                     YPLEAGGYATLSGTSMSAPHVAGAVALLLQARPGVRAADVRDLLQNTSEPKRWSGNPG
                     LGFLDPVHRQGAGMIDIVSAIQSPLHVSPGKLSLGEGEAGPHAVRLTLRNDGDEDVTL
                     APSFVNALTSSRSIAQTGTANAPGPNPGLGLFLGNAGVAFGSAAVTVPAGGVATLDAV
                     ITPATAPDNAMYGGFIVLTPNGGGETVRIPYGGFVGDYQAIQPLDGHIIQVGADLFLS
                     GFPWLTRLVGTSYQNLPDGGTFTLVDGDVPYFLVHFGHHVERLEFQVVNASNERPVHP
                     VFSNFVEIDDIGRNATRTQFFALTWDGTRFHSAGNPDLYKVVPNGDYKVNIKALKPLG
                     DPSNPDHWQVWTSPTVTIARPEEPKKKTINLKGKGPK"
     assembly_gap    7334..7343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7375..9672)
                     /locus_tag="PLS34_04870"
     CDS             complement(7375..9672)
                     /locus_tag="PLS34_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRP86837.1"
                     /translation="MMIQKTVPGVLLGLFLLSVSASQAGEPVIIKCAGGCDAAADSVL
                     AAGGSITYRYKYVDAIAATVPAQLFDRGSNLQGVERVLKDLVIDAPQAVESVTLEDDF
                     VVLDSAALELSPQDYNESVALTGVGPLFAGGHLGDGVKVAVIDSGTAPVSAFGRGGCS
                     VTDPTVIGGETFIAAAGPTEPSATSTLNGSHGTQVGTQIAANTIFGFANAAVISQSTV
                     AHSPSSAIVDIVPGVTGIPMVGTAPCAQIYALKTFPASGGGAPRSDIAAAMERAIELR
                     ENFNNGVPSAPVSGSGTAADPYVYDSLKIDVVNMSLGGPTTYAGYEITDQLTLKMLEV
                     GITVVNSAGNAGHAAMTGGSAGTGFGTLTAGAANLTHNERILRDVQFGLGIGALYRAS
                     DHHQVADFSSRGPSADGRINVDAVAGGLANLAQSPTGGIGLVSGTSFSAPTIAGAAAV
                     LHGALPGVSALQVRNALVETADPDVLGDNSAPIDRGHGFINVAAAYQALVNGQVSNKL
                     PSGPESNAVRGNIASLGIKTLQAKGRGTRETINGLVPGQVAHFFVNTTTETEAIVIRL
                     SNIRPELPPEEQNFFFGDDLFVLVQDAITSTDAVLDGGFIADDTALVALNPQSGILRI
                     AVMGDWTNAGRISADIEIQEFSGRKLKNSARDRVREGDALFYSVTVPNGTGQASFELQ
                     WQQDWGRYPTDDLDLIILTPGGDVVVDGATLNSPERAVIDNPVPGEYIVIVDGYTVWG
                     KRGSQAQFSLHAFDDAGRGFKTKEL"
     gene            complement(9865..>11150)
                     /gene="ettA"
                     /locus_tag="PLS34_04875"
     CDS             complement(9865..>11150)
                     /gene="ettA"
                     /locus_tag="PLS34_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRP86838.1"
                     /translation="LENLLQASGGHEADRTMEIAADALRLPPWDADVTKLSGGERRRV
                     ALCRLLLSRPDMLLLDEPTNHLDAESVAWLEHFLHDYSGTVIAVTHDRYFLDNVAGWI
                     LELDRGHGIPWKGNYSSWLEQKEQRLEQEAKSEGARIKAMKQELEWVRTNPKGRQAKS
                     KARMARFEELSTREYQQRNETNEIYIPPGPRLGDQVIEAKNLKKGFGDRLLFDNLSFE
                     LPKGAIVGVIGPNGAGKTTLFRMIIGTEQPDAGEIVRGSTVELAYVDQSRDALDPKKT
                     VWEEISEGQDIIRVGNYETPSRAYVGRFNFKGTDQQQKIGNLSGGERNRVHLAKVLRA
                     GGNLLLLDEPTNDLDVETLRALEEALLEFPGCAVVISHDRWFLDRIATHMLAFEGNSE
                     VVFFEGNYADYEADLKRRKGEEAAQPHRLRYKKII"
BASE COUNT         1675 a         3778 c         3854 g         1823 t
ORIGIN      
        1 gcattttcag ttcgctgtcg agatccagcc cgtagccgct cttgatctcc acgcaggtca
       61 cgccttccgc cgccagcgcc gccagccggg gcagggcgag cgccaccagt tcgtcctcgt
      121 cggcggcgcg caccgcctcc acggtggaca ggatgccgcc gcccgcgcgc gcgattccgg
      181 catagccggc gcccgcctgg cgcatggcga actcgtcggc gcgatcgccg gcccacacca
      241 ggtgggtgtg gcagtcgatc agcccgggca tgaccagccg cccgccgaga tcgaccgcac
      301 gcgcggcgga taccgcctcg cccagcgaca cgatgcgccc ctgttcgacc gcgaagcagt
      361 cgaggacctc gtcgccgtcg gccttcgggt agactctcgc gttgtgaaaa aacagctctg
      421 ccatggagtg gccggttccg ccggcgaacg aatcgatgtc acgtgaactc aatttcgagc
      481 atattctcac gcccgccggc atcgagcgcc accgcagcct gcgcatcgat gggcacggcc
      541 gcatcagcgc catcgaaccg tgcgcgcccg gcgggccctt cgacggctgg ctggcgctgc
      601 ccggcatgcc cagcgcgcac agtcacgcct tccagcgcgg tctggcgggc cacggcgaag
      661 cggcccgcgg cgcggacagc ttctggagct ggcgcgaggc gatgtaccgg ctggccgcgg
      721 gactggatgc ggacgcactc tacgcactgt ccttccaggc ttaccgcgag atgctggcgg
      781 ccggcttcac ctcggtggcc gagttccact acctgcaccg gcgggcggac ggcgagcgcg
      841 ccaccgagct gatcgaggcg gtgatccgtg ccggttcgga cgccggcatc cggctcgcgt
      901 tcatcccggt gttctaccag cgcgccggct tcgacacgcc ggccggtccc ggacaggcgc
      961 gcttcctgca ccgcgacgtg gaggatttcc tgacgctggt ggcgatgtat gccgagcact
     1021 gcgccggtgt cgccgcgcac tcgctgcgcg ccgtgccgcc cgagatgctc gccgacatgg
     1081 cgctcggcgc aggggagctg ctgggggcgg accgcccgat tcacatccac attgccgagc
     1141 agccggccga ggtggaggcc tgcctggcgg cgaccggccg ccggccggtg cagctcttgg
     1201 ccgacaccgt cgaactggga ccgcagtgga gcctggtgca cgccacccat gcggacgccg
     1261 cggagcgcgc gcggatcatc gccggcggcg cgaccgtggt gctgtgcccg ctcaccgaag
     1321 cctacctcgg tgacggcctg ttccccgcgg ccgagttcgc ggcggccggt ggccggctgg
     1381 ccatcggctc ggacagcaac gtgcgcatcg acgccatcga ggaattgcgc tggctcgaat
     1441 acggccagcg cctgctgacc gggcggcgcg gctgctttgc cgatgccgcc gggctgggtg
     1501 gcgccctgtg gcggcgcctc gcggccggcg tccctcgtca gactcgcgcg aaccggaaag
     1561 ctccaaactt ccggcgcgaa cgggtttcgg cgcgaatccg ccgccaaggg ttcccggatt
     1621 actttgggaa atttcggccc gtccaagccc ccaactccat nnnnnnnnnn cgaggcggtg
     1681 ctcgatgcgc tcgtcacggc cggcgacagc cgctgcctcg agcaggcctg ggtcggcggc
     1741 cagcgaaatg cgccgctgga agagggtgat ttcggcacgg cggtgcgacg catcatgggc
     1801 acggacggac gggcatgacg ccgccggggc ggacaccgac gcagcccgac gtcgcgccct
     1861 tcggcgagcg tgcgccgacg gcgccgccgg tgccgctggt tgtcagcatt ccgcacaccg
     1921 gcacctggct gccaccggag gtccgggaca gcctggcctc gcccgcgatg ctggcgcagc
     1981 cgatgaccga ctggcacctg cacctgctgt acgacttcct gccggcgctt ggcgccacca
     2041 ccatcttcgc cacggtcagc cgattcgccg tggacctcaa tcggccgcca cagccgcgcg
     2101 cgctttatcc gggccgcttc gagaccgggc tggtgccgct ggaaaccttc cagggcgagg
     2161 cggtattccg cagcccgccc gacgccgacg aggtcgaacg gcgcaggcgc gcctggcatg
     2221 cgccctacca cgcgcggctg caggcgctgc tggacgagac ccgggcgcgc ttcggccgcg
     2281 tggtgctggt cgacgcacac agcgtggcgt ccgcgccgaa ccgcttgcac ggtgcgctcg
     2341 aagacgagat ctacctgggc gatcgcgacg gcgcgacctg cggcggctgg ctgcgtgaac
     2401 tgctgcggga cggcttcgtt gcccagggcc tgtcggtggc gctcaatgcg ccctacaagg
     2461 gcggctacat cacggaccac tatggccgtc aggcgggcgt cgaggccatc cagatcgaaa
     2521 tggcccagcg cgtctacatg gacgagtccg acccggcggg cgggcccgag catccccgct
     2581 ttgctgcagc acaacagcga ttgctgcgcg tgctcgggca attggccgac aagctgtgaa
     2641 ttggggaatg tccccaaatc agggcttggt gaagcgcagc gtcatgcggt cgctttcgcc
     2701 gatcgccagg tatttctcgc gatccttgtc gccatgccgc agcgtcggcg gcagggccca
     2761 cacgccgccc tcgtggtcgg ccgtgtcctt cgcattggcg ttgatctccg cgctgccggc
     2821 aagcttgaag ccgcccgcct cgaactgcgc caccacccag tcctgcggca ggtagccgct
     2881 gtccttgccc gcctcgggat cgctgccggc cggcgcccga tgctgcacca cgccgagggt
     2941 gccgccgctc ttcaggaccg cgaaggcgtc ggcgatgaag gcctcgaggt tgccctggcg
     3001 gtacaggccg tgcaggttgc ggaaggtcag aaccaggtcc gcgctgcccg gcgcgccgag
     3061 cgcgtcgtcg ctgccggtct tgtagccgat cacctcgact tcgtcgtaaa ccgccggcgc
     3121 agcggccagc atggccagga actcgccgcg cacccgctgc atgtaggcgg agtcggaatc
     3181 ctccgggaaa tgcgccgcca ccagcttgcc gtggccgcgc aacaccggcg cgaggatctc
     3241 ggtgtaccag ccgccgcccg gcgaaacctc caccacggtc tggccctgcg cgaagccgaa
     3301 gaactcgagc gtctccccgg ggtggcgggc cccgtcgcgg gcgacattgg cctcggcgcg
     3361 gtgactgccg gcgattgcct tggccagtgc atcgtggtcg tgggcgccgg ccggggctgc
     3421 catcagcgtg gcggccagca gggcggtcgg gatgatccag gatttcatgg atggctctcc
     3481 tcggaatgcc gggtcttgcg agaagcgaac tatagcaagc ccggccgggg aggcgggttg
     3541 cgggacttgt gttcgccgcc gctaacccgg aagattcgcg ccccatggcg atcgagactt
     3601 ttcccgtccg gctcgagtgg cgccgcatgg cgacgccgca cgtgctgcac ctggcgttcc
     3661 gcaagagcga cgacggcacg ctggaatacg ctcccggcca gttcctcaac ctgcatttcg
     3721 aagcggcctc gggtggcacg caccgcagtt acagcatcgc caacccgccc ggccctgacg
     3781 gcctgctgga aatcgcgatg tcgccggtgg cgggcgggct ggcgaccgag gccctgtcca
     3841 cgctggcgcc gggcggcgag ctgcaggcca gcggcccgtt cggccgcttc gtgctgcgcg
     3901 aagagccgcc ctgccgctac gtgctggtcg gcacgggcac cggcatcacg ccttaccgcg
     3961 ccatgctgcc gctgctcggc gagcggctca aggccggttt ccgcgcgcac gtggtgctcg
     4021 gcgtctggcg gcgcgaggag ttgttgttcg gcgcagactt catcgccgcg gcgcggacgc
     4081 ttcccggctt cgagttcacc ggctgctaca gccgcgagct gcccgccgat cccgagccct
     4141 gggaacgccc tggctacgtg cagacgacgt acagcagcct ggatctcgac ccggaaaagg
     4201 acgtcgttta tctctgcggc aacccgggca tgatcgacga atcggtcgag atcctgaagg
     4261 ccatgggatt caccctcaag cagctgcggc gtgagaagta cctgtcggcg cgaccggctc
     4321 ggtagcaagg tgcagcctgg cggggcgcct gtggcaccga ccggccacaa atgaaaaggg
     4381 cctcccccga aggggaggcc ctgggccccg caggacggcg ggggagctag ccaggcaagg
     4441 ctatttcggg cccttgccct tgagattgat ggtcttcttc ttcggctcct ccggccgcgc
     4501 gatggtgacg gtcggcgagg tccagacctg ccagtggtcc gggttcgacg ggtcgccgag
     4561 cggcttgagc gccttgatgt tgaccttgta atcgccgttc ggaaccacct tgtacaggtc
     4621 cgggttgccc gcgctgtgga agcgcgtgcc gtcccaggtc aaggcgaaga actgcgtcct
     4681 cgtggcattg cggccgatgt cgtcgatctc gacgaagttc gagaacaccg gatgcacggg
     4741 ccgctcgttc gatgcgttga ccacctggaa ctcgagccgc tccacgtgat gcccgaagtg
     4801 gacgaggaaa tagggcacgt caccatcgac caaggtgaag gtgccgccgt ccggcaggtt
     4861 ctggtagctc gttccgacca gccgggtgag ccacgggaag ccgctcagga acaggtcagc
     4921 gccgacctgg atgatgtgcc cgtcaagcgg ctggatcgcc tggtagtcgc cgacgaagcc
     4981 gccgtacggt atgcgcacgg tttccccgcc gccgttcggc gtcagcacga tgaacccgcc
     5041 gtacatggcg ttgtccggtg cggtggccgg ggtgatcacg gcatccagtg tcgccacgcc
     5101 gccggccggc acggtgacgg cggctgaccc gaacgccacc cccgcgttgc cgaggaacag
     5161 gccgagtccc ggattcggtc cgggtgcgtt ggcggtaccc gtctgggcga tcgagcgcga
     5221 gctggtcagc gcgttgacga agctcggcgc gagcgtaacg tcctcatcac catcgttgcg
     5281 cagcgtcagg cgcaccgcat ggggtccggc ctcgccctcg ccgagcgaca gcttgcccgg
     5341 ggagacgtgc agcgggctct ggatcgcaga cacgatgtcg atcatgccgg caccctggcg
     5401 atgcaccgga tcgaggaagc cgaggccggg gttgcccgac cagcgcttcg gctcagacgt
     5461 attctgcagc aggtcgcgca catcggccgc tcggacgccg gggcgcgcct gcagcaacag
     5521 ggccaccgcg ccggcaacgt gcggcgcgga catggatgtg ccgctcaagg tggcatagcc
     5581 acccgcctcc agcgggtagg tcgagtagat cgcgccgccg ggcgcgccga gatccggctt
     5641 gatctggaga tccggcgaca tgccccagga cgagaacgcc gagatcaggt cgccggtcgg
     5701 attcgggaag cgatcgatgc cgtccgtcca ggtgatggtc gccggtgcgg ccaggcccct
     5761 gaggaacagg ccgtccgcca gggaaatgcc gaccaccggc tgggaaaagc cctgcggcgc
     5821 accgagggtc ccggccacga taccggccac gttgttgtac accacgactg cggtggcgcc
     5881 ggcattgacc gcgttgaccg ccttttcggc aaagccgcag acgccgcgta ccaccagcgc
     5941 cgccttgccg gtcgggtcgg ccagcagggc gtcggtgttg caggcctggc cgatgtagac
     6001 gatctcctcg ctgcccgagg tcggagccgc gctggagaac gtcatggtct ggtagccgat
     6061 acggttgccg cccgcctcga agtacggggc gcgcacccgg gcattgtcga aggaggcgac
     6121 cccgatgacg tcccggccga cgcccggtgc gccgatcgag aaagtgccgg tcgccccctg
     6181 gttgccggcc gaggcgatga cgaccacgcc ctgctcgacc aggcgatcgc cgacggtcgc
     6241 ggtcgggtag ctgggccact ggaatgcagc accgaggctc tggttgatga cctgcatgcc
     6301 gtccgcgtag gcacgctcga gcgccgcgac gatgacgtcg gcgctggagg agccaccgca
     6361 gccaaacacc ttgtaggcgc ccagcgtaac gtccggcgcc acgccggtaa cgccgtcttc
     6421 agcagcggcg ctcgcgccca cgatgccggc gacgtgcgtg ccgtgaccgt tgcaatcgtc
     6481 cggatcgtcg tcgggtatgg gcaggttgtt cgcgggatcg cccgcgttgt atgcatcgcc
     6541 gacgaagtcg aagccgtagg cgaccttgca gccaggtccg aaacagccgc cgagatcggg
     6601 atggtcgtaa tcgataccgg tgtcgacgat cgccaccttg atgcctgcgc cggtgagtcc
     6661 cagctcgctg cgggcgatgt ccactcgggt ctgggcgatg gcggaggcga cgtcgccgac
     6721 gctgccgctg tcgggggcgg gcggcgcatc gaactccacc acggggtaca cggccttgac
     6781 gcccgggatg gagcgcagcg tcgcggcgcc ggatgcggaa gtctcgaccg agacgccgtt
     6841 ccacagcgtc tggtagcgga atcgctccct gagttcgact ccggccgcgc ctgctgcgcg
     6901 ccggaaggcg tcgtgctcgc gttgcacggc gcccggcgaa acgccccgca ccgtcggcgg
     6961 cccggcaaac tcgacgaacc accgcgtggg cgatacgtcg tgctcgatgg atacctgcga
     7021 tacgtcggcg accgcaaggt catcggcgtg cgaaagcggc gcaaccgaca tcgcggtggc
     7081 caccgccgcc gacaatgaca cggcgagtga tgaaagcttc attggacccc cttggataag
     7141 aaaattggac gtcagccgtc cgtggccgct tcgtgcttct tggttctgcg gcggcgatct
     7201 tggtcgccgc tcgagcatcg aaagctacct atcccgggaa cgcctgacaa gcactatttg
     7261 ctgtacggca tggagcgggt gcaccggcgg gcagatcggt gctggtaaag aaaagcgggg
     7321 ccggcttttg gctnnnnnnn nnnccccgca actcaccgat cggtctcgat cgtgtcaaag
     7381 ttccttggtc ttgaaacccc ggcccgcgtc gtcgaaggcg tgcagggaga actgcgcctg
     7441 gctgccgcgt ttcccccata cggtgtagcc gtccacgatg acgatgtact cgccagggac
     7501 gggattgtcg atcacggcgc gctcggggct gttcagcgtg gcgccgtcga ccaccacgtc
     7561 accaccgggt gtcaggatga tcaggtcgag gtcatccgtg ggatagcggc cccagtcctg
     7621 ctgccattgc agctcgaagc tggcctggcc ggtcccgttc ggcaccgtga ccgaatagaa
     7681 cagtgcgtcg ccctcccgca ctcgatcccg ggcggaattc ttgagcttgc ggccgctgaa
     7741 ttcctggatc tcgatgtcgg cggaaatccg tcccgcattc gtccagtccc ccatcaccgc
     7801 gatcctgaga atgccggatt gcggattgag cgccaccagc gcggtgtcgt cggcgatgaa
     7861 gccgccgtcg agcacggcgt cggtggaggt gatggcatcc tggaccagga cgaacaggtc
     7921 gtcgccaaag aagaagttct gctcctccgg cggcagctcg ggccggatgt tgctcagccg
     7981 gatgacgatc gcctcggttt cggtggtcgt gttgacgaag aaatgcgcca cctgcccggg
     8041 cacgaggcca ttgatggttt cgcgcgtacc gcgccccttg gcctggagcg tcttgatgcc
     8101 gaggctggcg atgttgccgc gcaccgcgtt gctttccggg ccggacggca gcttgttgct
     8161 gacctgcccg ttcaccagcg cctggtaggc cgccgcgacg ttgatgaagc cgtggccacg
     8221 gtcgatcggt gccgaattgt cgccgagcac gtccggatcg gcggtttcga ccagcgcatt
     8281 gcggacctgc agtgcggaga cgcccggcaa cgcgccgtgc agcacggccg ccgccccggc
     8341 gatggtcggc gcagagaagg acgtgccgct gaccaggccg atcccgccgg tcggcgactg
     8401 ggccaggttg gccaggcccc cggccacggc atccacgttg atccggccgt ctgccgacgg
     8461 gccgcgcgaa ctgaagtccg ccacctggtg atgatcgctg gcgcggtaca gcgcgccgat
     8521 gccgaggccg aactggacgt cgcgcaggat gcgctcgttg tgcgtcaggt tggcggcacc
     8581 cgctgtcaac gtgccgaagc cggtgcccgc gctgccgccg gtcatcgccg cgtggcccgc
     8641 attgcctgcg gaattgacca ccgtgatccc gacctcgagc attttcagcg tcagctggtc
     8701 ggtgatttcg tagccggcgt aggtcgtggg cccgccgagg ctcatgttca ccacgtcgat
     8761 cttcagcgag tcgtagacgt aggggtccgc cgccgtgccg gagccggaca ccggcgcgga
     8821 gggcacgccg ttgttgaagt tctcccgcag ctcgatggcg cgttccatcg ccgcggcgat
     8881 gtccgatcgc ggcgcgccgc cgccggacgc cgggaaagtc ttcagcgcat agatctgcgc
     8941 acagggcgcc gtgccgacca tcgggatgcc ggtcacgccg ggcacgatgt cgacgatggc
     9001 gctcgacggc gagtgggcca ccgtggactg ggaaatcacg gcagcgttgg cgaacccgaa
     9061 aatcgtattg gcggcgatct gcgtgcccac ctgggtgccg tgcgacccgt tgagcgtgct
     9121 ggtggcggaa ggctcggtcg gtccggccgc cgcgatgaag gtctcgccgc cgatcacggt
     9181 cgggtcggtg acgctgcagc ccccgcggcc gaaggcggag accggtgcgg tgcctgaatc
     9241 gatcacggcc accttgacgc cgtcgccgag gtgcccgccg gcgaacagcg ggcctacgcc
     9301 cgtgagcgcc accgactcgt tgtagtcctg cgggctcagc tcgagggcgg cgctgtccag
     9361 cacgacaaag tcgtcctcca gagtgacgga ctcgaccgcc tgcggcgcat cgatcaccag
     9421 gtccttcagg acgcgttcga cgccctgcag gttactgccg cggtcgaaaa gttgtgccgg
     9481 cacggtggcc gcaatggcgt cgacatactt gtagcgatag gtgatcgagc cgccggcggc
     9541 gagcacgctg tcggcggccg catcgcagcc tcccgcgcac ttgatgatta cgggctcgcc
     9601 cgcctggctg gcggaaacgg atagcaggaa gagtccgagc agtacgcccg gaacggtttt
     9661 ctggatcatc atcttggctc cccttggcaa gacaggtcgg ttcgtccgcc ttccgtggcg
     9721 gcttatcttg ttgttgaacg gccgccgcgc atcgactgcc ggcgggccgt gcaaccgaaa
     9781 ctacccggca catccccgga actcaagtcg ggcgggggag cgcgcccgac caactcaaca
     9841 taatccagcc ctttgtgcag cgtctcagat gatcttcttg tatcgaagcc ggtgcggctg
     9901 ggccgcttct tcgcccttgc ggcgcttcag gtccgcctcg tagtcggcgt agttgccctc
     9961 gaagaacacg acttcgctgt tgccctcgaa ggcgagcatg tgggtggcga tgcggtcgag
    10021 gaaccagcgg tcatgcgaaa tgaccaccgc gcagccgggg aactccagca gcgcctcttc
    10081 cagcgcgcgc agcgtctcca cgtcgaggtc gttggtgggc tcgtcgagca gcagcaggtt
    10141 gccgccggcg cgcagcacct tggcgagatg cacgcgattg cgttcgccgc cggagaggtt
    10201 gccgatcttc tgctgctggt cggtgccctt gaaattgaag cgcccgacgt atgcgcgcga
    10261 gggcgtctcg tagttgccca cgcggatgat gtcctgcccc tcggagattt cttcccagac
    10321 ggtcttttta gggtccagcg cgtcgcggct ctggtcgacg taggcgagct ccacggtgga
    10381 gccgcgcacg atctcgccgg cgtccggttg ctcggtgccg atgatcatgc ggaacagggt
    10441 ggtcttgccg gcgccgttcg ggccgatgac gccgacgatg gcgcccttgg gcagctcgaa
    10501 ggagaggttg tcgaacagca gccgatcgcc gaagcccttc ttgagattct tcgcttcgat
    10561 cacctggtcg ccgaggcgcg ggccgggcgg gatgtagatc tcgttggtct cgttgcgctg
    10621 ctggtactcg cgggtggaca gctcctcgaa gcgggccatg cgcgccttgc tcttggcctg
    10681 gcggcccttc gggttggtgc gcacccactc gagctcctgc ttcatggcct tgatgcgcgc
    10741 accctcgctc ttggcctcct gctcgaggcg ctgctccttc tgctccagcc aggacgaata
    10801 gttgcccttc caggggatgc cgtggccgcg gtcgagctcg agtatccagc cggcaacgtt
    10861 gtcgaggaag tagcggtcgt gcgtcaccgc gatcacggtg ccggaatagt cgtgcaggaa
    10921 gtgttccagc caggcgaccg actcggcgtc gaggtggttg gtgggctcgt cgagcagcag
    10981 catgtccggc cgcgacagca agagccggca cagtgccacg cggcggcgct caccaccgga
    11041 cagcttcgtg acgtccgcgt cccacggcgg caggcgcagc gcgtcggcgg cgatttccat
    11101 ggtgcggtcg gcctcgtgcc cgccggaagc ctgcagcagg ttttccagct
//
LOCUS       DAOMPG010000061        11028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2537_length_11028_cov_4.579240, whole genome shotgun sequence.
ACCESSION   DAOMPG010000061 DAOMPG010000000
VERSION     DAOMPG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_11028_cov_4.579240"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLS34_04880"
     CDS             <1..374
                     /locus_tag="PLS34_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRP86839.1"
                     /translation="HINKTDSAVRITHLPSGIVVECQDDRSQHRNRAQAMSVLAARLN
                     DIQVRERQASEAATRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYRLDEILAGDL
                     DQVIEPLAAEYQADQLAALGV"
     gene            394..1398
                     /locus_tag="PLS34_04885"
     CDS             394..1398
                     /locus_tag="PLS34_04885"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014529584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRP86840.1"
                     /translation="MGVLPTVLEAIGNTPLIRLRGPSEETGCEILGKAEFMNPGGSVK
                     DRAALGIVRDAEARGQLKPGGVIVEGTAGNTGIGLALVGNALGYRTVIVMPETQSQEK
                     IDTLRAYGAEVRLVPAVPYKNPDNYVHVSQRLAEELARKEAEGALWANQFDNTANYAF
                     HLTTTGPEIWNQTEGRIHGFICAVGTGGTLAGVGRALKARDYHVQIGLSDPTGSALYN
                     HFAHGELKAEGGSITEGIGNGRITRNFAEAVVDHAFQVPDSESIPLVFRLLQEEGLLL
                     GGSSGVNVAGAMRLARKLGPGHTIVTILCDSGLRYRQRLFNPEFLRSKGLPVPEFLAN
                     "
     gene            complement(1503..3920)
                     /locus_tag="PLS34_04890"
     CDS             complement(1503..3920)
                     /locus_tag="PLS34_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HRP86841.1"
                     /translation="MNDKQVRRSVARGIRGSVTRTGIAVAAALAVLALGGPADVQARA
                     GAAKQATAGPGKTVQLSVLSSPPEFVSGGDARIEVRAAPGLHNKLELFLNGSRVHVPL
                     RARDSHHLEGVIEGLRDGENTIEVYVNRNSHRDTLRVTNYPVTGPMFSGPQQTPFVCT
                     TIQGAVGVQPLVDSATPPGYRVTDASGATIGYSRDCSIETVVRYFYRRAAGGGLVPLP
                     DDGSRPADMGTVTLADGRTVDFVVRREIGSINRFLYSIAMLAPMPAADNAAHNDTGYW
                     NGRLLYWFQGGVAIGHSQGTVHGGSMNPDVLGLGYAIVHSSGNNTGTHYNLQVAGETA
                     MMTKERFIERYGVPTYTVGLGGSGGAIQQYILGQNQPGILDALLPVQSYPDMVTQTIH
                     VGDCELLEYYMDATDRANPKWRTTKNRSWLVGFNAEDSRADPFVPLKLALGYSTAPGT
                     TECVPAWRGLTPLALNPLYGQAANQQNYAPITDILSIRWTHWDDLRNIYGVDETGWAR
                     VAWDNVGVQYGLDSLKAGNITPDEFLHLNWHIGGWKHPRDMVQEGFPFFGTSQAEIDK
                     IFADPSYFDPWSSRNMMRSDDPAQPAPRTVGDPLAMRNIYTGGMVFTGQLDRPTIDHR
                     QYMERELDMHNVHQSFAVRKRVQDRMGNSDMLVIWFTDTMPGVPKASQSMDAIAVMDE
                     WMANILANPKRSIRENRPARAVDSCFDLQGDLIYAGDDAWDGILDDKPAGPCTQQFPI
                     YTTSRIVAGAPLEGGIYKCALKPVAQAVADGTYAPWLPTASQVATLQQIFPAGVCDYS
                     KPDQARP"
     gene            complement(4125..5267)
                     /locus_tag="PLS34_04895"
                     /pseudo
     CDS             complement(4125..5267)
                     /locus_tag="PLS34_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
     gene            complement(5299..6687)
                     /locus_tag="PLS34_04900"
     CDS             complement(5299..6687)
                     /locus_tag="PLS34_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP86842.1"
                     /translation="MKIYRNVLEMVGRTPMLELGRLDTGPCRLFLKLELVNPGGSIKD
                     RIGISMIEQAEQRGDIKPGDLLVEATAGNTGIGLALAAAQKGYRLVLVLPDKMSQEKI
                     FNLRAMGAEIVLTRSDVGKGHPEYYQDLAQSIARERGGYYVNQFGNPDNPRAHEQTTA
                     PEIWEQMDHDVDAIVVGVGSSGTISGMTRYFREVAPGVEFVLADPAGSILADYIRTGE
                     IKPGGPWLVEGIGEDFIPDICDLSMVGSAYTVSDAESFSAARELLRREGVLAGSSSGT
                     LLAAALRYCREQTLPKRVVAFACDTGNKYLSKLYNDFWMEDQGFVQRPQHGDLRDLIG
                     RPHEHRATITVGADDVITTAHNRLRNAGFSQLPVMDGDQLAGVITEDDIIRYTFGRPA
                     RMGESVRSAMRTSFLKVDKATSVDNLVALLHGDSYAAVTEQEGTRFLGLITRSDVLNY
                     LRRLHQRERDEQ"
     gene            6779..7639
                     /gene="prmC"
                     /locus_tag="PLS34_04905"
     CDS             6779..7639
                     /gene="prmC"
                     /locus_tag="PLS34_04905"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRP86843.1"
                     /translation="MSDADFDLSIKQYLEDGASILRAVSDSPRLEAELLMGLVLRKPR
                     SYLHAWPEQQLSETDAERYEALLRQRFSGEPIAYMTGIREFWSMPLKVTPNVLIPRAE
                     TELLVEKALLKLPPDKDVRVLDLGTGSGAVSLAIARERPRATVIGVDISRAALEVARF
                     NARLQKIGNVEFRESDWFDAVRGEKFNVVVGNPPYVAEDDPHLAQGDARFEPRIALDA
                     GPGGMEAYRAILDRAHNYIVRQGWLLLEHGPTQHLPLRRLLEAQHYHDIAIHKDAAGH
                     DRVTECRFVE"
     gene            7644..8387
                     /locus_tag="PLS34_04910"
     CDS             7644..8387
                     /locus_tag="PLS34_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRP86844.1"
                     /translation="MTIDRYARHLALAGFGAAEQARLRDATALVIGAGGLGSPALLYL
                     AAAGIGRLVVSDFDTVDVTNLQRQVLFATDDVGRPKATIAAERLAALNPDTLVEAVPT
                     RLTDEALLDAVRAADVVLDCCDNFGTRFALNLACVQARKPLVSGAAIRFEGQLAVFRL
                     DRPGGPCYRCLWQEEAEELESCRGNGVLGPVPGVIGSLMAVEALKIASGCAPAAGGKL
                     LLYDAVENDWRSLKLERDPACPACKAGQW"
     gene            8465..10333
                     /locus_tag="PLS34_04915"
     CDS             8465..10333
                     /locus_tag="PLS34_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86845.1"
                     /translation="MSLLGELKRRNVFRAAAAYIAVAWLIVQVAETTFPAFDLTERAL
                     RTLILVLAAGFVPAVALAWVFELTPEGLKLDRDLEAGGELAVRTRRILDRAIVTLLAV
                     GLAYFTIDKFVFDPARDAAKIEAAQQRGRAAALVESYGDQSIAVLPFVNLSADPEQEY
                     FGDGMAEEILNVLARIPELRVISRSSAFTFKGKNLKLNEIAAELNVAHVLEGSVRRAG
                     NRIRVTAQLIEARSDTHLWSQTWDRELADIFAIQDEIAAEVVAALKLELLGAVPRARR
                     VDPEAYLLFMEARQMLDTSHDTAEEVDGLLTEAIGRDPEFAEPWTGLSWLYYRCHMLS
                     SRGIESPYCKRHSQETWLAMAVQARERALEIEPLNPTANAYRAYQRAFDERDIQGAAD
                     QFRRAVEAAPPVSDVLRTASIFARHIRRPHLAIRLGNFAMARDPLCLLCSYQLGLAHL
                     DAREFGQAEAHFEKFVRRGRGGWHTMAALRLLRDDPGGALEALERQPMDLQSPDKGSL
                     GVYLSRRAMALHGLGRMDEFQATLAMLETGLGPAAAEALAEVHAATGDLESASRWLAV
                     AMEGVPPTGAVLPINHLSPFLQPLLAQPRWQEVLREYGLADDQLAQIEFDFTLPGE"
     gene            complement(10338..>11028)
                     /locus_tag="PLS34_04920"
     CDS             complement(10338..>11028)
                     /locus_tag="PLS34_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP86846.1"
                     /translation="PTGAVYPRPLLEALARLAERHRLVLFSDEIYDRMTYDGAEHVPM
                     ATLVRDTLCGTFSGLSKVYRACGYRVGWVSFSGNRDTADRYIESVELLSALRLCANVP
                     GQWAVQTAIGGYQSIKELVGPGGRLHQSRQAIIDAAARSEFIELTPPAGAMYAFPRVR
                     TDELPDFDDQRFAFELLEHKHVLVAPGVSFNTPYNDHFRITTLPEPAVIGEVFARIED
                     LLHDWAVAEAS"
BASE COUNT         1752 a         3753 c         3690 g         1833 t
ORIGIN      
        1 aacacatcaa caagaccgac tccgcggtgc gcatcaccca cctgccgagc ggtatcgtcg
       61 tcgaatgtca ggacgaccgt tcccagcacc gcaatcgcgc ccaggccatg tcggtgcttg
      121 cagcgcggtt gaacgacata caggtgcgcg agcgccaggc cagcgaagcg gccacgcgca
      181 agagcctggt cggcagcggc gaccgctcgg agcgcatccg cacctacaat ttcccgcagg
      241 gccgcgtcac cgaccatcgc atcaacctga cgctctaccg gctcgacgag attctcgccg
      301 gcgatctgga ccaggtgatc gagccgctgg ccgccgagta ccaggccgac cagctcgcgg
      361 cgctcggcgt ctgagttcac aggaggcatt cgcatgggag tcctgcccac cgttctcgaa
      421 gccatcggca acacgccgct gatccgcctg cgcggaccct cggaagaaac cggctgcgaa
      481 atcctcggca aggccgagtt catgaacccc ggcggctcgg tgaaggaccg cgcggcgctg
      541 ggcatcgtgc gcgacgccga ggcgcgcggt cagctcaagc cgggcggcgt cattgtcgag
      601 ggcacggccg gcaacaccgg catcggcctc gcgctggtcg gcaacgcgct cggctatcgc
      661 acggtcatcg tcatgcccga gacgcagagc caggaaaaaa tcgacaccct cagggcctac
      721 ggcgcggagg tgcggctggt gccggcggtg ccctacaaga acccggacaa ctacgtgcac
      781 gtatcgcaac gacttgcgga ggagcttgcc aggaaggaag ccgagggcgc gctgtgggcc
      841 aaccagttcg acaacaccgc caactacgcc ttccacctga ccaccacggg tccggagatc
      901 tggaaccaga ccgagggccg gattcacggc ttcatctgcg ccgtgggcac cggcggtacg
      961 ctcgccggcg tcgggcgtgc gctcaaggcg cgcgactacc acgtgcagat cgggctgtcg
     1021 gaccccaccg gatccgccct ctacaaccat ttcgcgcatg gcgaactgaa ggccgagggc
     1081 ggctcgatca ccgagggcat cggcaacggc cgtatcacca ggaacttcgc cgaggccgtg
     1141 gtggaccacg ccttccaggt gcctgattcg gaatccatcc cgctggtgtt ccggctgctg
     1201 caggaagagg gcctgttgct gggcggctcc agcggcgtga acgtggcagg cgccatgcgc
     1261 ctggcgcgca agctggggcc cggccacacc atcgtcacca tcctgtgcga ttcgggcctg
     1321 cgctaccgcc agcgactgtt caacccggag ttcctgcggt caaagggcct gccggtgccg
     1381 gagttcctgg cgaattgact gtcccagccc tcgcccacat gagggtcgcc ggcaagatca
     1441 aatgtcgagg ggccgccggc ctgggccggc agcccctcgt cccttcgccc ccgttgcgag
     1501 cgctacggcc gggcctggtc aggcttgctg tagtcgcata cgcccgccgg gaagatctgc
     1561 tgcaacgtcg ccacctggct cgcggtcggt agccacggcg cataggtgcc gtcggccact
     1621 gcctgtgcca ctggtttcag ggcgcacttg tagatgccgc cctcgagcgg ggctcctgcc
     1681 acaatgcgcg aggtggtgta gatcgggaac tgctgggtgc agggtccggc cggcttgtcg
     1741 tccaggatgc cgtcccaggc gtcgtctccg gcgtagatca aatcaccctg caggtcgaaa
     1801 cagctgtcca cggcccgtgc cgggcgattc tcgcggatgc tccgtttcgg gttggccagg
     1861 atgttcgcca tccattcgtc catcaccgcg atggcgtcca tggactggct ggccttcggc
     1921 actccgggca tcgtgtcggt gaaccagatg accagcatgt cggagttgcc catgcggtcc
     1981 tgcacccgct tccgcacggc gaagctctgg tgcacgttgt gcatgtcgag ttcccgctcc
     2041 atgtactggc gatggtcgat ggttggccgg tccagctggc cggtgaacac cattccgccc
     2101 gtgtagatgt tgcgcatggc cagcgggtcg ccgacggtgc gcggtgccgg ctgcgccgga
     2161 tcgtcgctcc gcatcatgtt cctcgaactc cacgggtcga agtagctcgg gtccgcgaag
     2221 atcttgtcga tttccgcctg gctggtgccg aagaagggga agccctcctg caccatgtcg
     2281 cgcggatgtt tccagcctcc gatgtgccag ttcagatgca gaaactcgtc cggcgtgatg
     2341 ttgccggcct taagtgaatc gaggccgtac tgcaccccga cgttgtccca cgccacccgc
     2401 gcccagccgg tctcgtcaac gccgtaaatg ttgcgcaggt cgtcccaatg cgtccagcga
     2461 atcgacagga tgtccgtgat cggcgcgtag ttctgctggt tggctgcctg gccataaagc
     2521 gggttcaggg ccagcggcgt caggccacgc cacgcaggaa cgcactcggt ggtgcccggc
     2581 gccgtgctgt agccaagcgc aagcttcagc ggtacgaacg gatcggccct cgagtcttcg
     2641 gcgttgaaac cgaccagcca gctgcgattc ttggtagtgc gccacttcgg gttggctcgg
     2701 tcggtggcgt ccatgtagta ctcgagcagc tcgcagtcgc ccacatggat cgtctgggtg
     2761 accatgtcag gataggactg taccggcagc aacgcgtcga ggataccggg ctggttctgg
     2821 ccaaggatgt actgctggat ggcgccgccg gagccgccga gcccgaccgt gtaggtcggc
     2881 acgccgtagc gctcaatgaa ccgctccttg gtcatcatgg ccgtttcccc ggccacctgc
     2941 aggttgtaat gggtgccggt gttgttgccg ctggagtgca cgatggcgta gccgagcccg
     3001 agcacgtcgg gattcatcga gccgccgtgc accgtgccct gcgagtggcc gatcgccacg
     3061 ccgccctgga accagtacaa cagccgcccg ttccagtagc cggtgtcgtt gtgcgcagcg
     3121 ttgtccgccg ccggcatcgg cgccagcatg gcgatgctgt agaggaagcg gttgatcgag
     3181 ccgatctcac gccgcaccac aaagtccacc gtgcggccgt cagccagcgt gacggtgccc
     3241 atgtcggccg ggcggctgcc gtcgtccggc agcggcacca ggccgccgcc cgcggcgcgc
     3301 cggtaaaaat agcggacgac ggtctcgatc gagcagtcac ggctgtagcc gatggtcgcc
     3361 ccgctggcgt cggtcacgcg atagcccggc ggcgttgcgc tgtcgaccag tggttgtacg
     3421 cccacggcgc cctggatggt ggtacacacg aacggcgtct gttgcggtcc cgagaacatc
     3481 ggcccggtga cgggatagtt ggtcactcgc agcgtatcgc ggtgagaatt tcgattgacg
     3541 tacacctcga tggtgttctc gccgtcccgc aggccctcga ttactccctc gaggtgatgt
     3601 gagtcccgcg cacggagcgg aacgtgcacg cggctgccgt tgaggaacag ttcgagcttg
     3661 ttgtgcaggc ccggtgcggc ccgcacctcg atgcgtgcgt cgccgcccga tacgaattcc
     3721 ggcgggctgg acaagacgct cagctgcacc gtcttgcccg ggccggcggt ggcctgtttt
     3781 gctgcgcccg ccctggcctg tacgtcggcc ggcccgccga gtgccagaac ggccagtgcg
     3841 gcggcgaccg cgatgccggt gcgcgtcaca ctgcccctga tacctctcgc aacgctgcgc
     3901 ctgacttgct tgtcgttcat ctcccctcca cttcgtcatt atgttgtttt tgatgcgatt
     3961 gccgtggtcg cgtggccagc atccggatgc cacgccgacc tgccgcgggg agtgtactat
     4021 gcatgcagcg cagcctgtcc atcctgcgat tgcaacgggc tgccaggaag gcgcaatgct
     4081 gcggcgcaag acgccgccac gctcagcgct gttgacgggg gatgctagga actcgcgcgg
     4141 gaaagtgcag catccagcgc ctggtcgagg tccgccagca ggtcggcgca atcctcgatg
     4201 cccaccgaaa gcctgatcag cgaatcgctg atgccgagcc gcgcacgtgt ctcggcgggc
     4261 accgaggcgt gcgtcatgac cgccgggtga ttgacgaggc tctccacccc gccgaggctt
     4321 tcagcaaggg tgaacaaccg gcagtgctcc aggaaggcgc gagccgcatc gaagccgccg
     4381 gcgatatcga agctgacgat gccgccgcca cccgccatct ggcgacgcgc cagcgcgtgc
     4441 tgcggatgcg aatccagcca gggatagatg acgcgcagca cgccgcgacg cccctccagc
     4501 cagcgcgcga ccgccaggcc gttggcgctg tgtcgttcca tgcgcagttc gagggtcttc
     4561 acgccgcgca gcgccaggaa gctgtcgaac ggcccggcca cggccccgat cgagttctgc
     4621 aggaacgcga gttgctcggc gagacccgca tcgcgcaccg cgacgacccc gccgaccacg
     4681 tcggagtggc cgttcaggta cttggtcgcc gagtgcatca cgatgtcggc gccgagttcc
     4741 tggggacgct ggccccaggg gctggcgaag gtgttgtcca ccaccaccag cgcaccgctg
     4801 gcccgtgccc ttgccgctac cgctgcgatg tccaccagct tgagcatcgg gttggtcggc
     4861 gtctcgatcc acaccatgcg ggtgtccgcc tgcagcgcgc cctccagcgc cgccgggtcg
     4921 gacaggtcgg cataactgac tcgcagcccg gccgtacgcg cgcgcacccg gttgaacagg
     4981 cggccagtgc cgccgtagag atcgtccatg gcgaccacgt ggctgccggc gtcgagcaac
     5041 tcgagcaagg tggcgatggc cgccatgccg gaagcgaagg cgaatccgtg cgtaccgctt
     5101 tccaggtcgg cgaagcaacg ctcccaggcc atgcgggtgg ggttctgggt gcgcgagtac
     5161 tcgtaaccct ggtgcacgcc gggactggac tgcgcgtaag tcgacgttgc gtagatcggc
     5221 tgcatcaccg cgccggtgga cgggtcgggc gactggcccg cgtggatgac ccgcgtgccg
     5281 aaggcgtcgc gccgctcgct attgctcatc acgttccctc tggtggagtc gccgcaagta
     5341 attcaggacg tcggagcggg tgatcaggcc gagaaaccgc gtgccttcct gctccgttac
     5401 ggcggcatag gaatcgccgt gcagcagcgc caccaggttg tcgaccgagg tcgccttgtc
     5461 gaccttgagg aagctggtgc gcatcgcgct gcggaccgac tcccccatgc gcgccgggcg
     5521 gccgaaagtg tagcggatga tgtcgtcctc cgtgatgacg ccggccagct ggtcgccgtc
     5581 catcaccggc agctgcgaga agccggcatt gcgcaggcgg ttgtgcgccg tggtgatgac
     5641 atcgtccgcg cccaccgtga tggtggcgcg gtgctcgtgg ggccggccga tgagatcgcg
     5701 caggtcgccg tgctgcgggc gctgcacgaa gccctggtct tccatccaga aatcattgta
     5761 gagcttggac aggtacttgt tgcccgtgtc gcaggcgaac gccaccactc gcttcggcaa
     5821 cgtctgctca cggcaatagc gcaaggcggc cgcgagcaag gtcccggacg acgaaccggc
     5881 gagcacgccc tcccggcgca gcagttcgcg ggctgcggag aaggactccg catcgctgac
     5941 ggtgtaggcg ctgccgacca tcgacaggtc acagatgtcc ggaatgaaat cctcgccgat
     6001 gccctccacc agccagggcc ctcccggctt gatctcgccg gtgcggatgt agtccgcgag
     6061 aatcgagccg gccggatccg ccagcacgaa ttcgactccc ggcgccactt cgcgaaagta
     6121 acgggtcatg ccggagatcg tgccgctcga tccgaccccg acgacgatgg cgtccacgtc
     6181 gtggtccatc tgctcccaga tttcgggcgc ggtggtctgt tcgtgggccc tcgggttgtc
     6241 ggggttgccg aactggttga cgtagtaacc gccgcgctcc ctggcgatgc tctgcgccag
     6301 gtcctggtag tactccggat gtcccttacc cacgtcggag cgcgtcagga cgatctccgc
     6361 gcccatggcc cgcaggttga aaatcttctc ctggctcatc ttgtcgggca gcaccagcac
     6421 gagccggtag cccttctgcg ccgcggccag tgccagcccg atgccggtgt tgcccgccgt
     6481 cgcctcgacc agcaggtcgc cgggcttgat gtcgccgcgc tgctccgcct gctcgatcat
     6541 ggagatgccg atgcggtcct tgatggagcc gcccgggttc accagttcga gcttgaggaa
     6601 cagccggcag ggtccggtat cgagccgacc cagctcgagc atcggagtgc ggccgaccat
     6661 ctcgagcacg ttacggtaga tcttcatcgc ccctccgcgg cgccgcgccg gctgcgggag
     6721 tcgcgcggcc ggcaagctgc gcgtattcta cacgcccgat caggacatcg gaccgggcat
     6781 gagcgacgcg gacttcgacc tttccatcaa gcagtatctc gaggacggcg cgtccatcct
     6841 gcgcgccgtc agcgacagcc cgcgcctcga ggccgagctg ctcatgggcc tggtgctgag
     6901 gaagccccgc agttacctgc atgcgtggcc cgagcagcag ctctccgaga ccgatgccga
     6961 gcgctacgag gccctgctgc ggcagcgctt ctctggcgag ccgatcgcct acatgaccgg
     7021 catccgggaa ttctggtcga tgccgctgaa agtgactccg aacgtcctga ttccgcgggc
     7081 cgaaacggaa ttgctggtcg agaaggccct gctgaagctg ccgccggaca aggacgtgcg
     7141 cgtcctcgac ctcggtacgg gctcgggcgc cgtgtcgctg gcgatcgccc gcgaacgacc
     7201 gcgcgctacc gtcatcggcg tggatatttc ccgcgcggcg ctcgaagtcg cgcggttcaa
     7261 cgcgcgcctg cagaagatcg gcaacgtgga atttcgcgag agcgactggt tcgacgcggt
     7321 acggggcgag aaattcaacg tggtggtcgg caaccccccg tatgtcgcgg aggatgatcc
     7381 ccacctcgcc cagggcgatg cgcgtttcga gccgcgcatc gcgctggatg ccgggccggg
     7441 cggcatggag gcctatcggg ccatcctcga ccgggcgcac aattacatcg tgcggcaggg
     7501 ttggctgctg ctcgagcacg gcccgaccca gcacctgccg ctgcgccgac tgctggaggc
     7561 gcagcactac catgacattg ccattcacaa ggacgcggcc ggtcacgacc gggtgaccga
     7621 gtgccgcttc gtggagtgac ccggtgacca tcgaccgtta cgcacgccac ctggcgctgg
     7681 ccggattcgg cgcggcagag caggcgcgcc tgcgagacgc cacggcgctg gtgatcggcg
     7741 cgggcggtct cggctcgcct gccctgcttt accttgccgc ggccggcatc gggcgcctgg
     7801 tcgtcagcga cttcgacacc gtggacgtga ccaacctgca gcgccaggtg ctgttcgcta
     7861 ccgacgacgt cggccgcccc aaggccacga tcgcggccga gcggctggca gctctcaacc
     7921 ccgacaccct cgtcgaagcc gtcccgaccc ggctgacgga cgaggcgctg ctcgacgccg
     7981 tgcgggccgc cgacgtggta ctcgattgct gcgacaactt cggcacgcgc ttcgccctca
     8041 acctggcgtg cgtgcaggct cgcaagccgc tggtatcagg ggcggcgatc cgcttcgagg
     8101 gccagctggc ggtgtttcgc ctcgaccggc ctggcgggcc gtgctaccgc tgcctttggc
     8161 aggaagaggc cgaggagctg gagagctgtc gcggcaacgg cgtgctcggt ccggtgcccg
     8221 gcgtcatcgg cagcctgatg gcggtggaag cactgaaaat cgccagcggc tgcgccccgg
     8281 cggcgggcgg caagctcctg ctctacgacg ccgtggagaa cgactggcgc agcctgaagc
     8341 tcgaacgcga ccccgcgtgt ccggcctgca aggcgggaca atggtgagct gagaggcgcc
     8401 ggcgcttcct cccgggaccg cattccgcca tcatcggcgg caaacccgac aaccggatca
     8461 cgccatgtcg ttgctggggg agctcaagcg ccgcaacgtt ttccgcgctg ctgcggccta
     8521 catcgccgtg gcatggctca tcgtccaggt ggcggaaacg acctttcccg ccttcgacct
     8581 caccgagcgg gcgctgcgca ccctgatcct cgtcctggcc gccggcttcg tgccggccgt
     8641 agccctcgcc tgggtgttcg aactcacccc cgagggtctc aagctcgacc gcgacctcga
     8701 ggcgggcggc gagctggcgg tccgcacccg gcggatcctc gatcgcgcaa tagtcaccct
     8761 gctcgccgtg gggctggcct atttcaccat cgacaagttc gtgttcgacc cggcccggga
     8821 cgcggcgaag atcgaggcgg cccagcagcg cggccgtgcc gcggcgctgg tcgagtccta
     8881 tggcgaccag tccatcgccg tgctgccgtt cgtcaacctg agcgccgacc ccgagcagga
     8941 gtacttcggc gacggcatgg ccgaggagat tctgaacgtg ctggccagga tccccgaact
     9001 gcgggtgatt tcccgctcct cggctttcac cttcaagggc aagaacctca agctcaacga
     9061 aatcgccgcc gagctcaacg tcgcccacgt cctcgagggc tcggtgcgcc gtgccggcaa
     9121 ccggatccgg gtcaccgcgc agctgatcga ggcccgctcc gacacgcatc tctggtcaca
     9181 gacctgggac cgggagctcg cggacatctt cgccatccag gacgagatcg ccgccgaggt
     9241 ggtcgccgcc ctgaagctcg agctgctcgg cgcggtgccc cgggcgaggc gcgtcgaccc
     9301 cgaggcctac ctgctgttca tggaggcgcg ccagatgctc gacacctcac atgacaccgc
     9361 cgaggaggtc gacggcctgc tgaccgaggc gatcgggcgc gaccctgaat tcgcggaacc
     9421 atggaccggg ctcagctggc tgtactaccg ttgtcacatg ttgagcagcc gcggtatcga
     9481 gtcgccctac tgcaaacgcc attcgcagga aacctggctc gccatggcgg tccaggcgag
     9541 ggagcgggcg ctggaaatcg agccgctcaa cccgactgcg aatgcctacc gcgcctacca
     9601 gcgagctttc gacgagcgcg acatccaggg tgccgccgac cagtttcgtc gtgcggtcga
     9661 ggctgccccc ccagtttcgg acgtgctgcg caccgcaagc atattcgcac gccacatcag
     9721 gcgcccccac ctggcgatcc ggctgggcaa tttcgccatg gcgcgcgacc cgctctgctt
     9781 gttgtgttct tatcagctcg gcctcgcgca tctcgatgcg cgtgagtttg ggcaggctga
     9841 ggcgcatttc gagaaattcg tgcggcgcgg acgtggcggc tggcacacca tggccgccct
     9901 gcggttgctg cgcgacgacc cgggcggcgc gctcgaggcc ctcgagcgcc agccgatgga
     9961 cctgcagagc cccgacaagg gcagcctggg agtctacctg tcgcgccgcg cgatggcgct
    10021 gcatggcctc gggcggatgg acgaattcca ggccaccctg gccatgctcg agaccggtct
    10081 cggccccgcg gccgccgagg cgctggcgga agttcatgcc gccaccggcg acctcgagtc
    10141 ggcctcgcgg tggctcgcag tggcgatgga aggcgtgcca ccgacgggcg ccgtgctgcc
    10201 gatcaaccat ctcagcccct tcctgcaacc gctccttgca cagccgaggt ggcaggaggt
    10261 gctgcgcgaa tacggcctgg ccgacgatca gcttgcgcag atcgagttcg acttcaccct
    10321 gccgggcgag tgacggctca ggaggcttcc gccaccgccc agtcgtgcag caggtcctcg
    10381 atgcgggcga acacctcgcc gatcacggcc ggctcgggca gtgtcgtgat gcggaaatgg
    10441 tcgttgtacg gcgtgttgaa gctgacgccc ggtgccacca gtacgtgctt gtgctcgagc
    10501 agctcgaagg cgaagcgctg gtcatcgaag tcgggcagtt cgtcggtccg cacgcggggg
    10561 aaagcgtaca ttgcgccggc cggcggcgtg agctcgatga actcgctgcg cgccgccgcg
    10621 tcgatgatgg cctggcggga ctggtgcaag cggccgccgg gcccgaccag ctccttgatg
    10681 ctctggtagc cgccgatggc ggtctgcacc gcccattgac ccggcacgtt ggcgcacagt
    10741 cgcagcgccg acaataattc gaccgactcg atgtagcggt ccgccgtgtc gcggttgccg
    10801 gagaaggata cccacccgac ccggtagccg caggcgcgat agaccttgga gagcccggag
    10861 aaggtgccgc acagggtatc gcgcaccagc gtggccattg gcacgtgctc cgcgccgtcg
    10921 taggtcatcc ggtcgtagat ctcgtcgctg aacagcacca ggcggtggcg ctcggccagg
    10981 cgcgccaggg cctctagcag cggccgcgga tagacggcgc cggtcggg
//
LOCUS       DAOMPG010000062        10957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2558_length_10957_cov_4.598697, whole genome shotgun sequence.
ACCESSION   DAOMPG010000062 DAOMPG010000000
VERSION     DAOMPG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_10957_cov_4.598697"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..449)
                     /locus_tag="PLS34_04925"
     CDS             complement(57..449)
                     /locus_tag="PLS34_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86847.1"
                     /translation="MSDKRFPVLRDVIVFQVKLLVDGLRDLLLSPVSIIAALIDLLVP
                     GDDGGKRFYGVVHFGRRTERWINLFGMADKHDPEAPPAGIDVLLADLEGLARDPARRE
                     EARARAQAIVERLRGGPRPPGGETGHRD"
     gene            536..2440
                     /locus_tag="PLS34_04930"
     CDS             536..2440
                     /locus_tag="PLS34_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRP86848.1"
                     /translation="MDVEVLEIRQHLARFAPFEGLSDTLLDEVAGRIDVVYFKAGSEI
                     LRRGQQIDELHYIRSGAVEVYRRNGALHNRLEEGDIYGHFSLLRNHRVHFPVKAIEDT
                     LIYLVPDETFQRLCAEDEQFADFVELERPRLRSTVEKHAGDATMTTTRVRKLITRLPV
                     IVAADDTVQEAARRMNEQHATAVLVLAEPDENPRYNITDDTGAPRRLLGILTDSDFRS
                     RVVAEGLPPGTPAGTLVQGHPFTVQSDETLQEAMLCMLRNNMQHLPVLHRRRPMGVLH
                     ISDIIRHQTHTSLYLVERIFSQGSAEALARLLPDVREAFVRMVEEGANSQMIGSALST
                     IGRSFTRRLLALAEEELGPPPVPYSFMALGAMARNAQSIVTDQDNALVLDDAFEPERH
                     DTYFAAMGKIVSDGLAACGYTYCTGDIMATNPKWRQPLRVWKQYFRDWIESPTPERLL
                     HSSIFFDLDSVHGLDSLVVQLQDLVAEKAPGSPFFLAAMARNASNRTPPLGFFRNFVM
                     EKDGKHNNTINLKRRGTAPMTDLIRVHALAGGSTAQNSFDRLNDIEKTDLLPSGVGDR
                     IRYALEFISMVRIRHQAISLQRDQAPTNSIEPANISSTERHNLKDAFQLLSDAQKFLQ
                     FRYPVPSRHG"
     gene            2458..3177
                     /locus_tag="PLS34_04935"
     CDS             2458..3177
                     /locus_tag="PLS34_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP86849.1"
                     /translation="MSEQRGPLAAMAWPERIARAAAAASDARLRRFLQDWKLRPDRPL
                     GDTPLVAMDFETTGLDSRRHSIVSIGVVPFTLERIQFSQRRHWLLKPRFNLQERSVTL
                     HRITHSDLAAAPDLRLVLDEVLGALAGRLPVVHYHHIERNFLDSALRHRLGEGLLIPM
                     IDTMALEARVYRQSLAAKFRRWLGGRPVSIRLHDSRLRYGLPAYQPHHAVLDALATAE
                     LFQAQVAARFGPTTPIGELWC"
     gene            complement(3174..4847)
                     /locus_tag="PLS34_04940"
     CDS             complement(3174..4847)
                     /locus_tag="PLS34_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRP86850.1"
                     /translation="MEHKPKQQSATERDKLSEGVPAAEGAHNPIDTDYVIGQDNYTAS
                     PLGIRLDIHGTVFAISAVVTLFFVVLTLALQNEIEPLFNAIRDWLTGNLAWFFILAGN
                     VFVVLCLALIFSPLGRIRIGGSEATPDFSYLGWFSMLFAAGMGIGLMFYGVSEPMSHF
                     DAALGGITMENGARTDWAPLGAAAGDPEGAARLGMAATIFHWGLHPWAIYAVVALALA
                     LFSFNKGLPLTMRSIFYPLLGERVWGWPGHVIDMLAVFATLFGLATSLGIGASQASAG
                     LSFLFGMPEGNVTMVALILGITLVATGSILLGVDKGVKRLSQVNMTLAFLLLLFVLIV
                     GPTVLILTGFFENLWSYAVNLPALANPFFREDANFSQGWTAFYWAWWISWSPFVGMFI
                     ARVSRGRTVREFLVAVLLVPSIVSVLWMTTFGGTAINQFVGSGFEGVRDAALELKLFV
                     MLGELPLTEITSFVGIVLVVVFFITSSDSGSLVIDTITAGGKVEAPKAQRVFWAFIEG
                     GIAVALLLGGGLVALQAMAVSTGLPFTLVLLAGCYAIVKGLLSEPRPAG"
     gene            complement(5153..6391)
                     /locus_tag="PLS34_04945"
     CDS             complement(5153..6391)
                     /locus_tag="PLS34_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86851.1"
                     /translation="MSSCSARSTVAAALLLLALAGGSASAQDIAVRAGVLHTGAGESF
                     APGVVVIRGGKIIAVGAADAVAIPAGMEVLEAPVATPGLIDARSVVGLAGAMNQAHDQ
                     DQLERSAAIQPELRAVDAYNPRELLVDWLREHGVTTLHTGHGPGEVISGQTLIAKTAG
                     GTVDEAVIRPVFALAATLGEQAAIGHARSSPGNRSKAVAMLREQLVKAGEYRVKRSGK
                     DESKRPPRDLALEALAQVLDGELPLVIEVHRHSDIMTALRLAKEFEFRLILSGASDAH
                     LLIDEIRTAGVPVLLHPTMMRARGPEVENFSFTTAARLLEAGIPVALQGGYEGYVPKG
                     RVVLFEAAMTLPYGLDFDQALSLVTSAPARILGIDDRVGTLEVGKDGDVALFGGDPFE
                     YTTQVIGTVIDGRRVSSTLR"
     gene            complement(6388..7716)
                     /locus_tag="PLS34_04950"
     CDS             complement(6388..7716)
                     /locus_tag="PLS34_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86852.1"
                     /translation="MHHRFVTAAALAAMLMLTLAALPAFAAGTAFVGARIVPVTGPAI
                     IDGVLLIENGKIAAVGPRDAVRIPRDARVVELAADQVLMPGIVDTHSHIGEIAGADGS
                     APIQPGVRVLDALNVRSSGFKRALAGGITTVNVMPGSGHLVSGQTLYLKLREANVIDA
                     LLILDDAGEPMSGLKLANGTNSLRAPPFPGTRAKSAALVRQQFIRAREYREARQRKDS
                     GDDGQDGGRDLDLETLVEVLDGKRMVHFHSHRHDDLMTAIRLRDEFGFRMVLHHTAEA
                     WRVADEIAAAKVPVSLILIDSPGGKLEALHLEGRSAPALEQAGVQVAFHTDDWITDSR
                     YFLRMAGMGIRAGMTPEGALRAMTVNGAQMLDLGGRVGSLEPGKDADFVILSGDPFSV
                     YTRVQQTWVEGVKRFDLADPDDRLVAEGGFGALVDDDGGNHLHEHHGAHR"
     gene            7833..8876
                     /locus_tag="PLS34_04955"
     CDS             7833..8876
                     /locus_tag="PLS34_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRP86853.1"
                     /translation="MSSRATPGLLVCAGTALAATAVPVLLSAAPGRWAGLPISPILVA
                     ILLGLVLAATARQRTAWAPGLALAAGPLLKLAVMLIGLRLSLGQVGSLGVQAAPLVLG
                     ALLAGLGLAWLLGRAVGLGRKFTALLAVGSAICGASAIAATAPALRARPEETAYALAC
                     VALFGLAATLVYPWLLDACFETGLPVGLVLGAAIHDTSQVTAAALLFEQTFDATGVVA
                     AATVTKLLRNLCMLVVIPAVVWWALQGETAGRARPAFPLFILGFLALAATRSGGDLLF
                     DGSTAWRMVLDTAATISAFLFAMAMAAIGMGIRLAALRALGVRPAVVALLTALGMGGV
                     ALVLAALLAGPGI"
     gene            complement(8908..9456)
                     /locus_tag="PLS34_04960"
     CDS             complement(8908..9456)
                     /locus_tag="PLS34_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86854.1"
                     /translation="MTQGTRRVRAGTLAAVVAASVFAGACSRADDAPFTIRDDGIGPL
                     VIGRDYDDAVEAARVVAPESILAGPGCGGLDEVRYQGRMGEFPVSLMAMAEDTVLVEV
                     ELGLDSPLTAQDEAACIALRNRFAAPFVARFGQPSAHWEIDKPVSREHLLGTGPVVVA
                     ARWFPTGRSCYISAHYGYPARE"
     gene            complement(9453..10013)
                     /locus_tag="PLS34_04965"
     CDS             complement(9453..10013)
                     /locus_tag="PLS34_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HRP86855.1"
                     /translation="MRAAAVVSALAIAGLAAGAVHAEHHGKKDGHTIKQATAEFIDKE
                     SKPVGTATLTESPHGVLISLDLHGLPPETKAIHIHSVGTCEDPAEGFVASKGHLNPDG
                     KEHGLMNPAGPDAGDLPNLVVRDDGSVKVEMFTMLASLGGAEGRATILDEDGAALVIH
                     INRDDHITQPIGGAGPRIACGVIKAE"
     gene            10213..>10957
                     /locus_tag="PLS34_04970"
     CDS             10213..>10957
                     /locus_tag="PLS34_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRP86856.1"
                     /translation="MSDREESTGQSFSRFVERVGHKIPDPVIIFMAFYPIAFLVTVLM
                     GGHTFETMGAGGEPVSYQILPMYQSEHVRWIFDNALLRNWLAFGNGVLAVILVVMLAI
                     GVAENSGLFAALIKRAGTHIPQAWLPLLLMFLGITSSVATDAGYLVLIPLAGLLYAGL
                     GRNPLIGMAAAFAGVSAGFSANLIPATPIDAIIGMNAQAFAEAQGVPFTKADGTPLTY
                     ATMNYFFIATSTVVLAITGAWVTRRFVEPK"
BASE COUNT         1759 a         3710 c         3715 g         1773 t
ORIGIN      
        1 ttgcctttgc tggcgcggat ccggcgggcc gcgccgccgt cgcgggggat ccgcggctag
       61 tccctgtgcc cggtttcccc gccgggcggc cgcgggccgc cgcgcagccg ctcgacgatg
      121 gcctgggcgc gcgcgcgggc ttcctcgcgc cgcgccgggt cgcgcgccag tccctcgagg
      181 tccgccagca gcacgtcgat gccggccggc ggcgcctccg ggtcgtgctt gtcggccatg
      241 ccgaaaaggt tgatccagcg ctcggtgcgg cgcccgaagt gcactacgcc gtagaaccgc
      301 ttgccgccgt cgtcgcccgg caccagcagg tcgatcagcg cggcgatgat ggacaccggg
      361 ctgagcagca ggtcgcgcag gccgtcgacc agcagcttga cctggaacac gatcacgtcg
      421 cgcaggactg ggaatcgctt gtcgctcatc tgggggccgc tccgtgccgt gaactcgtcg
      481 caagaccagg cttgttacac tccaaagtta gcaggaagca gggaggaccg gggccttgga
      541 cgtcgaagtg ctcgaaattc ggcagcacct ggcgcgattt gcgccattcg agggcctgtc
      601 ggacacgctg ctggacgaag tggcgggccg gatcgacgtg gtttatttca aggcgggcag
      661 cgaaatcctg cggcgcggcc agcagatcga tgaactgcat tacattcgca gcggtgcggt
      721 cgaggtctac cggcgcaacg gcgcacttca caaccggctg gaggaaggcg acatctacgg
      781 ccacttcagc ctgctgcgca atcaccgcgt gcattttccg gtcaaggcca tcgaggacac
      841 cctcatttac ctggtgccgg acgagacctt tcagcggctc tgcgcggagg acgagcagtt
      901 tgccgacttc gtcgaactcg aacgcccgcg gctgaggagc acggtcgaga aacacgccgg
      961 cgacgccacc atgaccacga cgcgggtacg caagctcatt acacgcctgc cggtgatcgt
     1021 ggccgccgac gatacggtac aggaagcggc gcgccgcatg aacgagcagc acgccaccgc
     1081 ggtgctggtg ctggccgagc ccgacgaaaa tccacgttac aacatcaccg acgatacggg
     1141 cgcaccgcgg cggctactcg gtatcctcac cgacagtgac ttccgcagcc gggtcgtggc
     1201 ggagggcctg ccgccgggga caccggccgg cacgctggtc cagggccatc cgttcacggt
     1261 gcagtcggac gagaccctgc aggaagcgat gctctgcatg ttgcgcaaca acatgcagca
     1321 cctgccggtg ctccatcggc gccggccgat gggcgtgctg catatctcgg acatcatccg
     1381 tcaccagaca cacaccagtc tgtacctggt cgagcggatt ttttcccagg gcagcgccga
     1441 ggcgctcgcc cggctgctgc cggacgtgcg cgaggctttc gtgcgcatgg tggaggaggg
     1501 cgcgaattcg cagatgatcg gcagcgcgct gtccaccatc ggccgcagct tcacccgccg
     1561 cttgctggcg ctggcggagg aggaactggg cccgcccccg gtgccctaca gcttcatggc
     1621 gctgggcgcg atggcccgca acgcgcagag catcgtcacc gaccaggaca atgcgctggt
     1681 gctggacgac gcgttcgagc ccgagcggca cgatacctat tttgccgcca tgggcaagat
     1741 tgtcagcgac ggcctggcgg cctgcggtta cacctactgc accggcgaca tcatggccac
     1801 caacccgaag tggcgccagc cattgcgcgt ctggaagcag tacttccgcg actggatcga
     1861 aagtcccacg ccggagcgcc tgctgcacag ctcgatcttc ttcgacctcg acagcgtgca
     1921 cggcctcgac tcgctcgtgg tccagttgca ggacctggtc gccgagaaag cgcccggcag
     1981 tccgttcttc ctggcggcca tggcgcgcaa cgcctccaac cggacgccgc cgctgggatt
     2041 cttccgcaat ttcgtgatgg agaaggacgg caagcacaac aacaccatca acctgaagcg
     2101 gcgcggcacg gcgccgatga ccgacctgat ccgggtgcat gcgctggccg gcggatccac
     2161 ggcacagaac tccttcgacc ggctcaacga catcgagaag accgacttgt tgccgtccgg
     2221 cgtgggcgac cggatccgct atgcgctgga gttcatctcc atggtgcgta tccgacacca
     2281 ggccatcagc ttgcagcgtg accaggcgcc gaccaacagc atcgagcccg cgaacatctc
     2341 gagcacggag cgccacaacc tgaaggacgc cttccagctg ctgagtgatg cgcagaagtt
     2401 cctgcagttt cgctatcccg tgccgtcgcg gcacggatga ggcgcggggc gatggaattg
     2461 agcgagcagc gtgggccact ggcagcaatg gcctggccgg agcgcatcgc gcgcgccgcc
     2521 gcggccgcca gcgacgcccg gctgcggcgt ttccttcaag attggaagct gcggcccgac
     2581 cggccgctcg gcgacacgcc gctggtcgcc atggacttcg agaccacggg actggattcg
     2641 cgtcgccatt ccattgtcag catcggcgtg gtgccgttca cgctggagcg cattcagttc
     2701 tcgcagcggc gtcactggct gctcaagccc cgcttcaacc tgcaggagcg ttccgtcact
     2761 ctccatcgaa tcacgcactc cgacctggca gcggcgccgg acctgcggct ggtgctggac
     2821 gaggtgctcg gagcgctggc cggacgcctg ccggtagtgc actaccacca catcgagcgc
     2881 aacttcctgg actccgcgct gcgccaccgg ctcggcgaag gcctgctgat cccgatgatc
     2941 gataccatgg cactggaggc gcgcgtctac cggcaatccc tggcggcgaa gttccgccgc
     3001 tggctgggcg gcaggcccgt ctccattcgg ctgcacgaca gccgcctccg ctacggcctg
     3061 ccagcctacc agccgcacca tgccgtgctg gacgctctcg ccaccgcaga gctgttccag
     3121 gcgcaggtcg cggcccgctt cggcccgacg acgccgatcg gcgagctgtg gtgctagccg
     3181 gccgggcgcg gctcgctcag cagtcccttg acgatcgcat agcatcccgc cagcagcacc
     3241 agcgtgaacg gcaggccggt ggagaccgcc atcgcctgca gtgcgaccag cccgccgccg
     3301 agcaacaggg caacggcaat gccaccctcg atgaaggccc agaacacgcg ttgcgccttc
     3361 ggcgcctcga ccttgccgcc ggcggtgatg gtatcgatca cgagtgatcc cgagtcggag
     3421 gaggtgatga agaacaccac caccagcacg atgcccacga aggaggtgat ctcggtcagc
     3481 ggcagttcgc cgagcatcac gaacagcttc agctccaggg cggcgtcgcg cacgccctcg
     3541 aatccgctgc cgacgaactg gttgatggcg gtgccgccga aggtggtcat ccacaagacc
     3601 gaaacgatcg acggcacgag cagcaccgcg accaggaact cgcgcaccgt gcggccgcgg
     3661 ctgacccgcg cgatgaacat gccgacgaac ggcgaccagc tgatccacca ggcccagtag
     3721 aaggccgtcc agccctggct gaaattggcg tcctcccgga agaacgggtt ggccagcgcg
     3781 ggcagattga ccgcgtagga ccaaagattc tcgaaaaagc ccgtcagaat cagcacggtc
     3841 ggcccgacga tcagcacgaa cagcagcagc aggaaggcca gcgtcatgtt gacctgggac
     3901 aggcgcttca cgcccttgtc gacgcccagg agaatggagc cggtggccac cagggtgatg
     3961 ccgaggatca gcgccaccat ggtcacgttg ccttccggca tgccgaacag gaaactcagc
     4021 ccggccgaag cctgcgacgc gccgatgccc agcgaggtgg ccagcccgaa cagggtggcg
     4081 aacaccgcga gcatgtcgat cacgtgcccc ggccaccccc agactcgctc gccgagcagg
     4141 ggatagaata tcgagcgcat ggtcagcggc aggcccttgt tgaacgagaa cagcgccagt
     4201 gcgagggcca ccacggcata gatcgcccag ggatgcagcc cccagtggaa gatcgtcgcc
     4261 gccatgccga ggcgggcggc gccctccggg tcgccggcgg ccgcgccgag cggcgcccag
     4321 tcggtccgcg cgccgttctc cattgtgatg ccgcccaggg ccgcatcgaa atgcgacatc
     4381 ggctccgaga cgccgtagaa catcaacccg atgcccatgc cggcggcgaa cagcatcgaa
     4441 aaccagccga ggtaggaaaa atcgggcgtc gcctccgagc cgccgatccg tatccggccc
     4501 agcggggaga agatcagcgc caggcagagc actacgaaga cgttcccggc gagaatgaaa
     4561 aaccaggcga gattaccggt gagccagtcc cgtatcgcgt tgaacagcgg ctcgatttcg
     4621 ttctgcagcg ccagggtcag cacgacgaag aacagcgtca ccacggccga gatcgcaaac
     4681 accgtgccgt ggatatcgag gcggatacct agcggcgacg cggtgtaatt gtcctgcccg
     4741 atgacgtaat cggtgtcgat cggattgtgc gcgccttcgg cagcgggaac gccctcgctg
     4801 agcttgtctc gttccgtggc agattgttgc ttgggtttat gctccatggg acttctccgt
     4861 ctggaggggt ttcgtgctca gggacggaac aggaacacgg acgaactggc gtgggccgcg
     4921 gctcgatgct cgtctggcag atagtcggcg tcggggtgac gagtcgggaa agcggccgtc
     4981 agcgggacga cggcgcccgg ccaggatgcg ggcacaggcg gaagcggcgg gagaaaccag
     5041 cgatttacgc gtgtctggga tgggtccgtg gccgtttttc tcgtcgttag gcgcgccatg
     5101 ttagcagctg gccgccgacc ctgctcacgg ttgcggtccc gcaagcctgc cttcagcgta
     5161 acgtactgct gaccctgcgg ccgtcgatca ccgtgccgat gacttgcgtg gtgtactcga
     5221 aaggatcacc gccgaacagt gcgacgtcgc catccttgcc gacctccagg gtcccgacgc
     5281 ggtcgtcgat gccgagaatg cgggccggtg cgctggtcac caatgacagc gcctggtcga
     5341 aatccaggcc gtagggcagg gtcatggcgg cctcgaagag cacgacgcgc cccttcggca
     5401 cgtagccctc gtaaccgcct tgcagcgcga ccggaatgcc ggcctcgagc aggcgagctg
     5461 cggtggtgaa cgaaaagttc tccacctccg ggccgcgtgc ccgcatcatg gtcgggtgca
     5521 gcaggacggg aacaccggcg gtccggatct cgtcgatcag caggtgcgca tcgctggcgc
     5581 cggacaggat cagccggaac tcgaattcct tcgccagccg cagcgcggtc atgatgtcgc
     5641 tgtggcgatg gacttcaatc accagcggca actccccgtc gagcacctgc gcaagtgcct
     5701 cgagggcaag atcccgtggc ggccgtttgg attcgtcctt gccgctccgc ttgacccggt
     5761 actcgcccgc ctttacgagt tgctcgcgca gcatggcgac cgccttgctg cgattgcccg
     5821 gcgaactgcg ggcatgcccg atggcggcct gctcgccgag cgtcgccgcc agcgcgaaca
     5881 ccggacgaat cacggcctca tccacggttc cgcccgcggt cttggcgatg agcgtctggc
     5941 cggagatcac ctcgcccggc ccgtggccgg tatgaagcgt ggtcacgccg tgttcgcgca
     6001 gccagtcgac cagcaattcg cgcggattgt aggcatccac ggcccgcagc tcgggctgga
     6061 tcgcggcgga tcgttccagc tggtcctggt cgtgcgcctg gttcatggcg ccggccaggc
     6121 cgaccaccga gcgcgcatcg atcagccccg gcgtcgcgac cggggcttcc aggacttcca
     6181 taccggcggg aatcgccacc gcgtccgcgg cgcccacggc gatgatcttg ccgccgcgga
     6241 tgaccactac gccgggcgcg aacgattcgc ccgcgccggt gtgcaacacc cccgcgcgca
     6301 cggcgatgtc ctgggctgac gcggagccgc cggccagcgc gagcagcagc aatgcggcag
     6361 caacggtcga tcttgcgctg cagctgctca tcggtgcgct ccatggtgtt cgtgcaggtg
     6421 attgccgccg tcgtcgtcca ccagcgcgcc gaagccgccc tcggccacca gccggtcgtc
     6481 cgggtctgcg aggtcgaatc gcttcacgcc ctcgacccag gtctgctgga cgcgggtgta
     6541 gacgctgaaa gggtcgcccg aaaggatgac gaagtccgca tccttgcccg gctccagcga
     6601 gcccacgcgc ccgccgaggt ccagcatctg cgcgccattc accgtcatcg cccgcaaggc
     6661 gccctccggc gtcatgccgg cgcggatccc catgcccgcc atgcgcagga agtagcgcga
     6721 gtcggtgatc cagtcatcgg tatggaaggc gacctgtacg ccggcctgtt ccagcgccgg
     6781 agcgctgcga ccttcgaggt gcagggcctc gagcttgccg cccgggctgt cgatcaggat
     6841 cagcgacacc ggcaccttgg ccgcagcgat ctcgtccgcg acgcgccacg cctcggcggt
     6901 gtgatgcagc accatgcgga agccgaactc gtcgcgcagg cggatcgcgg tcatcaggtc
     6961 gtcgtggcga tggctgtgaa agtgcaccat gcgcttgccg tcgagcacct ctaccagcgt
     7021 ctcgaggtcg aggtcgcgcc cgccgtcctg gccgtcgtcg ccggaatcct tgcgctgccg
     7081 tgcctcgcgg tactcacgcg cgcggatgaa ctgctggcgg accagcgccg ccgacttcgc
     7141 gcgcgtaccc ggaaagggcg gagcgcgcag cgagttggta ccgttggcca gcttcaagcc
     7201 cgacatgggc tctcccgcat cgtcgagaat cagcagggcg tcgatgacgt tggcttcgcg
     7261 cagcttgagg tacagcgtct ggccgctgac caggtggccc gagcccggca tcacgttcac
     7321 ggtggtgatg ccccccgcca gggcgcgctt gaagccggag gagcgcacat tgagcgcatc
     7381 gagcacgcgc acgccgggct ggatgggtgc cgagccgtcg gcgccggcga tctcgccgat
     7441 gtggctgtgg gtgtcgacga tgccgggcat gagtacctgg tctgccgcca gctccaccac
     7501 tcgtgcgtcc cgggggatcc gcaccgcgtc gcgcggcccg acggcggcga tcttgccgtt
     7561 ctcgatgagc agcacgccgt cgatgatcgc cggacccgtg actggaacga tgcgcgcgcc
     7621 gacgaaggcg gtgccggccg cgaaggcggg cagggcggcc agcgtcagca tgagcattgc
     7681 agccagggcg gcagcagtga caaagcgatg gtgcatgcag gggtcctctc tcaggcccgc
     7741 cggaaagtac cacagtctcc ccggggcaac gaaacccggg cttcccacgg ccgagcgaac
     7801 cggccatgct tgcgcgcatg gatttcgccc agatgagctc acgcgcgacg ccggggctgc
     7861 tcgtttgtgc gggtacggcg ctggccgcga ccgccgttcc cgtgctgttg tccgccgccc
     7921 cggggcggtg ggcgggcctg ccgatcagcc cgatcctggt ggcgatcctg ctgggcctgg
     7981 tgctggcggc gaccgcccgc cagcgcacgg cctgggcgcc gggcctggcg ctggccgcgg
     8041 ggcccttgct caagctggcg gtcatgctca tcggcctgcg gctgtcgctc gggcaggtgg
     8101 gcagcctggg cgtgcaggcc gcgccgctgg tgctcggcgc gctgctggcc gggctcgggc
     8161 tcgcctggct gctcgggcgg gccgtcggtc tcggccgcaa gttcaccgcc ctgctggcgg
     8221 tgggatcggc catctgcggc gcctcggcga ttgccgcgac ggcaccggca ttgcgggcac
     8281 gcccggaaga aacggcctat gcgctggcct gcgtcgccct gttcggcctg gcggcgacgc
     8341 tcgtctatcc ctggctgctc gatgcctgct tcgagacggg cttgccggtc ggcctggtcc
     8401 tcggcgcggc cattcacgac acctcgcagg ttacggcggc cgccctgctg ttcgagcaga
     8461 ccttcgatgc gacgggcgtg gtagccgctg ccacggtgac caagctgctg cgcaacctgt
     8521 gcatgctggt cgtgataccg gccgtcgtgt ggtgggcgct gcaaggcgag acggccggcc
     8581 gcgcccggcc ggcgttcccg ctgttcattc tcggcttcct ggcgcttgcc gcaacccgca
     8641 gcgggggcga cctgctgttc gacggcagca cggcatggcg gatggtgctg gacaccgcgg
     8701 cgaccatcag cgcgtttctg tttgcgatgg cgatggcggc catcggcatg ggtatccggc
     8761 tggccgcgct acgcgccctg ggcgtccggc ccgcggtcgt ggcgttgttg accgcactcg
     8821 gcatgggcgg ggtggcactg gtgctggccg ccctgctggc cggcccggga atctgaccgg
     8881 cgggcggcat cgcgccccgc attgcggtca ttcccgggcc gggtagccgt agtgggcgct
     8941 gatgtagcag cttcgcccgg tcgggaacca gcgcgcggcg accaccaccg gcccggtgcc
     9001 cagcaggtgc tcacgtgaca cgggcttgtc gatttcccag tgagccgagg gctggccgaa
     9061 gcgtgcgacg aagggcgccg caaaccggtt gcgcagggcg atgcatgccg cctcgtcctg
     9121 tgccgtcagc ggcgagtcca gcccgagctc gacctcgacc agcaccgtat cctcggccat
     9181 cgccatgagg ctgaccggaa attcgcccat ccggccctgg tagcggacct cgtccagccc
     9241 gccgcagccc ggtccggcca gtatggactc cggggccacg acacgtgcgg cttcgaccgc
     9301 atcgtcatag tcgcggccga tcacgagcgg cccgatgccg tcgtcgcgaa tcgtgaaggg
     9361 agcgtcgtca gcgcgggagc aggcgccagc aaaaacgctc gccgccacga cggcggcgag
     9421 cgttcccgcg cggacccggc gggtcccctg cgtcattcgg ccttgatgac tccgcatgca
     9481 atgcgcgggc cggcgccgcc gatcggctgg gtgatgtggt cgtcgcgatt gatgtggatg
     9541 accagcgccg caccgtcctc atcgagtatg gtggcccggc cctcggcgcc gccgagcgag
     9601 gcgagcatgg tgaacatctc gaccttgacc gagccgtcgt cgcgcaccac caggttcggc
     9661 aggtcgccgg cgtccggccc ggcgggattc atcaggccgt gctccttgcc gtcgggattc
     9721 aggtggccct tggatgcgac gaagccttcc gccggatcct cgcaggtgcc gacgctgtgg
     9781 atgtggatgg ccttggtctc cggcggcagg ccgtgcaggt cgagggagat cagcacgcca
     9841 tgcgggcttt cggtcagcgt ggccgtcccg accggcttgc tttccttgtc gatgaattcc
     9901 gccgtggcct gtttgatcgt gtggccgtcc ttcttgccgt ggtgctcggc gtgtaccgca
     9961 ccggcggcca ggcctgcgat ggcaagtgcg gaaactactg ctgctgctct catgtgacga
    10021 aacctcgtgg atgtttttgt ggtggtggtc ggcgcagccg ccttgggcgc ggccagggaa
    10081 accatttaag actgattcta gatcaaatcg acggcgactg cgcgacaggc gggaatgctg
    10141 aggccggatc cgtcttttct gcgggtatac ttcgcgcctg ttgtgccaga gagcgaatag
    10201 ctggggagcg cgatgagcga cagggaagaa agcaccggcc agtctttcag tcgattcgtg
    10261 gagcgcgtcg gccacaagat cccggacccg gtgatcatct tcatggcgtt ctacccgatc
    10321 gccttcctgg tcacggtgct gatgggcggg cacaccttcg agaccatggg tgcgggtggc
    10381 gagccggtca gctaccagat cctgccaatg taccagtcgg agcacgtgcg ctggattttc
    10441 gacaacgcac tgctgcgcaa ctggctggcc ttcggcaacg gcgtgctcgc ggtgatcctg
    10501 gtggtgatgc tggcgatcgg ggtcgccgag aactcggggc tgttcgcggc gctgatcaag
    10561 cgggccggta cacacattcc acaggcctgg ttgccgttgc tgctgatgtt cctcggcatc
    10621 accagttcgg tcgccaccga cgccggctac ctggtgctga ttccgctggc gggcctgctg
    10681 tatgcaggcc tcgggcgcaa cccgctgatc ggcatggcgg cggcgtttgc cggggtgtcg
    10741 gcgggcttca gcgccaacct gatcccggcc acgcccatcg acgccatcat cggcatgaac
    10801 gcccaggcct tcgcggaggc ccagggggtg cccttcacga aagccgacgg cacaccgttg
    10861 acctacgcca cgatgaacta ctttttcatc gccacctcga cggtcgtttt ggccattacc
    10921 ggggcctggg tcaccaggcg cttcgtcgag ccgaagc
//
LOCUS       DAOMPG010000063        10870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2597_length_10870_cov_4.146001, whole genome shotgun sequence.
ACCESSION   DAOMPG010000063 DAOMPG010000000
VERSION     DAOMPG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_10870_cov_4.146001"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..541
                     /locus_tag="PLS34_04975"
     CDS             134..541
                     /locus_tag="PLS34_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRP86857.1"
                     /translation="MNCRGFTLLEVLVALLLLSLAVSSVLGLALHGFATTTEARRAEI
                     AGALAADLAGRVRAFATVDWTALPAPAPCATTCPPEQAAAVELADWRAAVAAALPDGA
                     ALLAPGPGGELLLTLAWTETGGVPRELRLGIAQ"
     gene            538..1383
                     /locus_tag="PLS34_04980"
     CDS             538..1383
                     /locus_tag="PLS34_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HRP86858.1"
                     /translation="MKRTSHIAGVSLVELLVALGIGLALSLAMATLHARVSHLYGRTT
                     AAADAQDTLRIALATLEYELAHAGYWGLVPDAAHIAGRRGDVAPLAAAVAGDCGPAWA
                     IDLDRPLVGWSGGWPLACAPFAGAAPVSGGLVLRRVATAPRTPDAGVLQVYGDPWGGR
                     LGVSGAPAAPGESVHDLVARAYYVSPRATADPTRPSLRRKTLQRGPRVIDEEMVVGIA
                     RMDVALGVDTDAPGDPGHGQPNRFVAPELASGEVVAVRVRLTSDEDARLSLTRTIALR
                     NGPVP"
     gene            1380..1838
                     /locus_tag="PLS34_04985"
     CDS             1380..1838
                     /locus_tag="PLS34_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86859.1"
                     /translation="MKRTSAGCAQRARGGVLALLLVLLAAGMALSAAVATSAALELAM
                     SDRAVDRLRAAEAAEAGIAAALRARGWSSASPWTATGNLPESGRWQVEVRLFAARVDP
                     HGGPVDWRFEIESAGEHGAARVTLLQSFDVTGALPGEPRPVAWRQLEPSP"
     gene            1835..2245
                     /locus_tag="PLS34_04990"
     CDS             1835..2245
                     /locus_tag="PLS34_04990"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HRP86860.1"
                     /translation="MRRGFTLIELLICIVVVALLATIAMPSWRDVLARSRRADAAAAL
                     YAVAAAQERYRLVHGRYASDAGPAPPVGLGLDRSERGWYALRIETADMTRFIASARPA
                     AGSPQATDTACRIMTIDEAGRRGSTPAPPETCWR"
     gene            complement(2264..3202)
                     /gene="ispH"
                     /locus_tag="PLS34_04995"
                     /gene_synonym="lytB"
     CDS             complement(2264..3202)
                     /gene="ispH"
                     /locus_tag="PLS34_04995"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRP86861.1"
                     /translation="MKILLANPRGFCAGVDRAIDIVERALQMFGAPIYVRHEVVHNRY
                     VVDRLRSLGAVFVEELDEVPDGATVIFSAHGVSKAVQAEAERRGLEVFDATCPLVTKV
                     HIEVSRYGREGREVILIGHAGHPEVEGTMGQYDRSKGGDIYLVENPEDAARLEVRDPE
                     HLAFVTQTTLSVDDTTRVIEALKARFPALTSPRKEDICYATQNRQDAVKALVARCDAI
                     VVVGSKTSSNSSRLKEIALKAGIPGYMVDGADDLRREWFDDCRTVGVTAGASAPEVLV
                     QQVVARLKEWGGELPEEIRGREEHVVFSLPKVLRPA"
     gene            complement(3235..6498)
                     /locus_tag="PLS34_05000"
     CDS             complement(3235..6498)
                     /locus_tag="PLS34_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRP86862.1"
                     /translation="MRPVLLLTIALALIAPSTGTAETRLLRFPDINGDRVAFVHGGDI
                     YSAFVQGGVAQRLTSHEGQELYPKFSPDGRWIAFSAEYSGTRQVWVMPSAGGTPRQLT
                     FYSDVGPMPPRGGTDYRVLDWTPDGKHVLVRANRTPYGERDGVPMLVPFEGGMERPLG
                     PPETGGGMLSGDGNTYVYTPIDREFRTWKRHRGGRAQNVWTYDLANNTSRQLTDFIGT
                     DHQPMWVDGRVFFVSDRDFTLNLFVMDMDGGNLSKVTDFTEFDVLWPSSDRKRVVFEN
                     GGYIWLHDPAAGETRRLDIAVRGDKPHTLAGFRNVTKQIESFDIAPGGERAVFGARGE
                     IFTAPAQHGEIRNISNTPDAREISVAWSPDGKWISYLSDATGEYEIYVRSQDGSGAPK
                     RVTSDGSVWRFPPVWSPDSKKLAFADSANRLHFVEVESGRVRQVDTTRHTSNSFRDLT
                     QYVWSPDSQWLAYTKVNESYNSSIWVYSLREAKPIQLTADATNEQSPAFDPEGRYLYF
                     TSTRDWNLNFSAYEFNYLYHNATRIFAATLAADGPALNRPRSDEVGAGGKDESGNSSK
                     ADDKKPAADKPLKFDLQGFNERVTALDVPAGNHVGLAASKGGVFFVSTGGNGAELRYY
                     DLEKRAAESVLAGVTGYSLSADGKKLLWRQQDKFGIADAKPGIEADKTALKLERLEMR
                     VDPQREWQQMYVDGWRILRDWFWDPGHHGQDWEAIRERYAPLVEHVAHRADLDYIFGE
                     LAGELNAGHIYVNSSEDTPGAKRQPGGMLGARIEAHESGYFRIAHVYPGENWHDYYRS
                     PLTEAGVKAREGDFILAVDGVSTRGVDNFYRLLENKGDRVVELTLNGRPEERGARVER
                     VRTVTSEVNLRYLDWVTENRRRVHAMSGGRIGYIHVPNTAIEGNRELFKGMLAEARRD
                     ALIIDDRYNGGGFIPDRMIEMLARTPLNYWVWRGFEPQPRPLLSHDGPKAMLINGLAS
                     SGGDALPYYFRKLGLGPLIGTRTWGGLIGISGNPGLADGGSIIPATFRFLDTDGNWAV
                     EDEGVAPDIEVVDRPEAIAAGRDPSLEKAVEVLLEALEKNPPRRIEAPPAPTDFRFD"
     gene            complement(6556..7074)
                     /gene="lspA"
                     /locus_tag="PLS34_05005"
     CDS             complement(6556..7074)
                     /gene="lspA"
                     /locus_tag="PLS34_05005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRP86863.1"
                     /translation="MSEQAYPAGRERGALGWLWLSAFVVVADQLSKAYIVATFELYDR
                     VQLLPVFGITRLHNTGAAFSFLASAGGWQRWFFVAIAVAVTALVCVWLKRMPRRGQAW
                     LAASLALVVGGAIGNVIDRLFRGHVVDFISVHYDRWFFPAFNVADSALTVGAAILLIE
                     SFYDGRRKKKII"
     gene            complement(7071..9896)
                     /gene="ileS"
                     /locus_tag="PLS34_05010"
     CDS             complement(7071..9896)
                     /gene="ileS"
                     /locus_tag="PLS34_05010"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRP86864.1"
                     /translation="MADYKDTLNLPRTDFPMRAALAQREPDMLADWEARNLYGKLRAA
                     ARGRPAFLLPDGPPYANGDIHLGHCVNKVLKDIVIRSRTLDGYDAPYIPGWDCHGLPI
                     ELVVEREHGKVGAKLDAKAFRDACRAFARSQVDGQRRDFRRLGILGDWERPYLTMDPA
                     YEAEQLRAFAKILANGHVYKGFKPVHWCLDCGSALAEAEVEYADKKSPAIDVRYRVVD
                     EAALLAAFGGAARDDVAGPASIPIWTTTPWTLPASQAVALHPELEYALISFDGPVGRE
                     RLVVARDLLDATTRRWGIEHFEELAVCRGAALEGVVLQHPFLAREVPVILGEHVTTEA
                     GTGAVHTAPGHGHDDFIVGQRYGLEVDNPVLDDGRFRTGTEAVAGLKVFDANPVIVEL
                     LEARGALLRHEPMLHSYPHCWRHKTPLIFRATPQWFVGMERAGLRERALQAIGEVKWM
                     PGWGENRIRAMVEGRPDWCVSRQRTWGVPIALFVDRDSGAPHPDSVRLLQEVADRVEK
                     EGIDAWWSLEPAELLGDEAARYEKVQDIMDVWVDSGLMHHCLSATREEVGYPAELYLE
                     GSDQHRGWFQSSLLTAVAMHGTAPYRAVLTHGFTVDEKGRKMSKSLGNVVAPQKVLNS
                     LGADVLRLWVAATDYRGEMNVSEEILKRMSDSYRRMRNTARFLLGNLDGFDPASHAVA
                     PEDMIALDRWAVARTAALQEEVVQAYRDYEFHLIYQKVHNFCVLDMGGFYLDVIKDRL
                     YTTPADGQPRRSAQTAMYHVAEAMVRWLAPVLAFTAEEIWSSLPGERAESVLLSGWYE
                     LPEVPLREDDPDWDLVHAVRDDVARELEALRNAGRIGSSLDAELVLYAADDLAARLRR
                     PGDELRFLLLTSTARVEPLDARPADAIELEGFAARLYAQVLPTDHAKCVRCWHRRADV
                     GVDPDHPEICGRCAVNVSGAGEQRRWA"
     gene            complement(9911..10846)
                     /gene="ribF"
                     /locus_tag="PLS34_05015"
     CDS             complement(9911..10846)
                     /gene="ribF"
                     /locus_tag="PLS34_05015"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005301192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRP86865.1"
                     /translation="MELIRGKHNLRPGHRGCAVTIGNFDGLHLGHRAVLERLTGKARA
                     LGVAAVVMSFEPTPREYFMGAAAPPRLARFREKFVGLKALGVDRLFCARFDANMAALE
                     PEDFIREYLVDGLGTRYLVIGDDFRFGRNRAGNFASLQAAGREHYFEVEDTPTVVLDG
                     LRVSSTAVRAALAEGDLGLAARLLGRHYGMTGRVIEGRRLGQTLGFPTANLRPGRRVL
                     PVTGVFAVLVRGVGERPWPAVANLGTRPTVDGTEPLLEAHLFDFSGDLYGRRITVDFV
                     ARLRDEQRFDDIAGLKAQMHRDAKLARRILADAGY"
BASE COUNT         1581 a         3866 c         3749 g         1674 t
ORIGIN      
        1 gagcgcgaac gccagtcgct ggagcccttg ctggcgacgc ccgcggcgcg cgggcggcgc
       61 gcgccgtgat cattgccccc accggccggc cgcgcctgga acgcggttcg ggctcactgc
      121 cctgtcccgg cccatgaact gccgcggctt caccctgctg gaggtgctgg tggccctgct
      181 gttgctgtcc ctggcagtga gcagcgtgct ggggctggcg ctgcacggtt tcgctacgac
      241 gaccgaagcg cgccgcgcgg agatcgcggg cgcgctggcg gccgacctgg ccggccgggt
      301 gcgcgcgttc gcgaccgtgg actggactgc gctgccggcg ccggccccct gcgcgaccac
      361 ctgcccgccg gaacaggcgg ccgcggtgga gctggccgac tggcgcgcag cggtcgccgc
      421 ggcgctgccc gacggcgcgg cgctgctggc tcccggaccc gggggcgaac tgctcctgac
      481 gctcgcctgg acggagaccg gcggggtgcc gcgcgagttg cgcctgggaa tcgcgcaatg
      541 aagcgcacgt cgcacatcgc gggcgtctcg ctggtcgaac tgctggtggc gctcggcatc
      601 ggccttgcgc ttagcctggc gatggccacc ctgcacgccc gagtcagcca tctttacgga
      661 cggaccacgg cggctgccga tgcccaggac accttgcgta tcgcactggc gacgctggag
      721 tacgagctgg cgcacgccgg ctactggggc ctggtcccgg atgccgcgca tattgccggc
      781 cgccgtggcg acgtcgcacc gctggcggcc gccgtggccg gcgattgcgg tcccgcctgg
      841 gcgatcgacc tcgaccggcc gctggtcggc tggtcgggcg gctggccgtt ggcctgcgcg
      901 ccctttgccg gcgcggcgcc ggtgagcggc ggcctggtgt tgcggcgtgt cgccacagcg
      961 ccgcgcacac ccgatgccgg cgtgctccag gtatacggcg acccgtgggg cggacgcctc
     1021 ggcgtgagcg gcgccccggc cgcgccgggc gagtccgtgc acgacctggt ggcgcgtgcg
     1081 tactacgtca gtccccgcgc caccgccgat ccgacgcggc cgtccctgcg ccgcaagacg
     1141 ttgcagcgcg gcccgcgcgt gatcgacgag gagatggtcg tcggtatcgc ccgcatggac
     1201 gtcgcccttg gcgtggacac cgatgcgccc ggcgacccgg gacacggcca gccgaatcgc
     1261 ttcgtcgccc ccgagctggc gagcggcgaa gtcgtcgcgg tgcgggtccg cctgaccagc
     1321 gacgaggacg cgcgcctgtc gttgacgcgc accattgcgc tgcgcaacgg gccggtgccg
     1381 tgaagcgtac atccgccggc tgcgcccaac gcgcccgggg cggcgtgctg gcgctgctgc
     1441 tcgtgctgct ggccgccggc atggcgctgt ccgcggccgt cgcaacctca gctgctctgg
     1501 aacttgcgat gagcgaccgg gcggtggacc ggctgcgcgc ggccgaggcc gccgaggccg
     1561 gcatcgccgc cgcgctgcgc gcgcgaggct ggtcgagcgc gagcccgtgg acggccaccg
     1621 gcaacctgcc cgagagcggc cggtggcaag tcgaggtacg cctgttcgcc gcccgggtgg
     1681 acccgcacgg cggcccggtc gactggcgct tcgagatcga aagcgccggc gagcacggtg
     1741 ccgcccgggt gaccttgctg cagtccttcg atgtcaccgg cgcgctgccc ggcgagccgc
     1801 ggcccgtggc ctggcgccag ctcgagccct cgccgtgaga cgcggtttca cgctgatcga
     1861 gctgttgatc tgtatcgtcg tcgtggcgct gttggcgacc attgccatgc cgtcgtggcg
     1921 cgacgtgctc gcccgcagcc gccgcgcgga cgccgccgcc gcgctctacg cagttgccgc
     1981 cgcacaggaa cgctaccggc tggtgcacgg ccgttacgcc agcgatgcgg ggcccgcacc
     2041 cccggtcggc ctcggcctcg atcggagcga gcggggctgg tatgcgttgc gcatcgagac
     2101 ggcggacatg acgcggttca tcgccagcgc acgcccggcc gccggctcac cgcaggcgac
     2161 ggacaccgcc tgccggatca tgaccatcga cgaagccggc cgccgcggct cgacgccggc
     2221 gccgccggag acctgctggc gctgaggccg cgaaccgcgt cgctcaggcc ggtcgcaaca
     2281 ccttgggcag ggaaaacacg acgtgctctt cgcgaccccg gatctcctcc gggagctcgc
     2341 cgccccactc cttgagacgc gccacgacct gctgcaccag cacctccggc gccgatgcgc
     2401 cggccgtgac gccgaccgtg cggcagtcgt cgaaccactc ccggcgcagg tcgtcggcgc
     2461 cgtccaccat gtagcccgga atgccggcct tcagggcgat ctccttcagg cgactggagt
     2521 tggagctggt cttcgagccc accaccacga tcgcgtcgca gcgtgccacc agcgctttca
     2581 cagcgtcctg gcgattctgg gtggcataac agatgtcttc cttgcgtggg ctggtgagcg
     2641 caggaaaccg cgccttcagc gcttcgatga cgcgcgtcgt gtcgtccacc gacagcgtcg
     2701 tctgggtgac gaacgccagg tgctccggat cgcgaacctc gaggcgcgcg gcgtcctcgg
     2761 ggttctccac caggtagatg tcgccgccct tgctgcgatc gtactggccc atggtgccct
     2821 cgacctcggg atgccccgca tgcccgatca ggatcacttc gcggccttcc cggccgtagc
     2881 gggacacctc gatgtgcacc ttggtgacca gcgggcaggt ggcgtcgaac acctccagcc
     2941 cgcggcgctc ggcctccgcc tgcaccgcct tggacacgcc gtgcgcgctg aagatcaccg
     3001 tggcgccgtc cgggacctcg tccagctctt cgacgaacac cgcgccgagg ctgcgcagcc
     3061 ggtccaccac gtagcggttg tgcacgacct cgtgacgcac gtagatgggc gcgccgaaca
     3121 tctgcagggc gcgctcgacg atgtcgatcg cccggtcgac gccggcgcag aagccacgcg
     3181 ggttggcgag caggattttc attggcctga tattaaccca agcgtcgcaa ggcttcagtc
     3241 gaagcggaaa tcggtgggcg ccgggggcgc ctcgatgcgc cgcggcgggt tcttctcgag
     3301 cgcctccagc aggacttcga ccgccttctc gaggctcggg tcgcgcccgg cggcaatcgc
     3361 ctccggccgg tccacgacct cgatgtcggg cgccacgcct tcgtcttcca cggcccagtt
     3421 gccgtcggtg tcgaggaaac ggaaggtcgc cgggatgatg ctgccgccgt ccgccaggcc
     3481 gggattgccg ctgatgccga tcagcccgcc ccaggtgcgc gtgccgatca gcggcccgag
     3541 tcccagcttg cggaagtaat acggcaacgc gtccccgccg gagctggcca ggccgttgat
     3601 gagcatggcc ttcggcccgt cgtggctgag cagcggccgc ggctgcggct cgaaaccgcg
     3661 ccacacccag tagttcagcg gcgtgcgcgc cagcatctcg atcatgcggt ccgggatgaa
     3721 gccgccgccg ttgtagcggt cgtcgatgat cagcgcgtcg cgtcgcgcct ccgccagcat
     3781 gcccttgaac agctcgcgat tgccctcgat ggcggtgttc ggcacgtgga tgtagccgat
     3841 ccgcccgccc gacatggcat gcacccggcg gcggttctcc gtgacccagt cgaggtagcg
     3901 caggttgacc tcgctggtca ccgtcctgac gcgctccacc cgcgcaccac gctcttccgg
     3961 ccggccgttc agcgtgagct cgacgacgcg gtcgcccttg ttctccagca ggcggtagaa
     4021 attgtcaacg ccgcgcgtgc tgacgccgtc caccgccagg atgaagtccc cctcgcgggc
     4081 cttgacgccc gcttcggtga gcggcgagcg gtagtagtcg tgccagttct cgcccgggta
     4141 gacatgggcg atgcggaaat agcccgactc gtgcgcctcg atgcgtgcgc cgagcattcc
     4201 gccgggctgc cgcttggccc cgggggtgtc ctcgctgctg ttgacgtaga tgtgcccggc
     4261 gttcaactcg ccggccagct cgccgaaaat gtagtcgagg tcggcgcgat gcgccacgtg
     4321 ctcgaccagc ggcgcgtagc gttcgcgaat ggcttcccag tcctggccgt gatggcccgg
     4381 atcccagaac cagtcgcgca ggatgcgcca gccgtccacg tacatctgct gccactcgcg
     4441 ctgcgggtcg acgcgcatct ccagccgctc cagcttgagc gccgtcttgt cggcctcgat
     4501 gcccggcttg gcgtccgcga tgccgaactt gtcctgctgc cgccagagca gcttcttgcc
     4561 gtccgccgac aggctgtagc ccgtgacgcc ggcgagcacg ctctcggccg cgcgcttctc
     4621 gaggtcgtag taacgcagct ccgcgccatt gccgccggtg gacacgaaga acacgccgcc
     4681 cttgctggcg gcgagaccga catggttgcc ggcgggaacg tccagcgcgg tgacgcgctc
     4741 gttgaagccc tgcaggtcga acttgagcgg cttgtcggcg gccggcttct tgtcgtcagc
     4801 cttcgacgag ttcccggact cgtccttgcc acctgcgccg acttcgtcgc tgcgcgggcg
     4861 gttgagcgcc ggcccgtcgg ccgccagggt ggcggcgaag atccgcgtgg cgttgtgata
     4921 gagataattg aactcgtacg cggagaaatt caggttccag tcgcgggtcg aggtgaaata
     4981 aagatagcgg ccctccgggt cgaaggccgg gctctgctcg ttggtggcgt ccgcagtcag
     5041 ctggattggc ttggcctcgc gcagcgaata gacccagatc gagctgttgt agctctcgtt
     5101 gaccttggtg tacgccagcc actggctgtc gggcgaccag acgtactggg tcaggtcacg
     5161 gaaggagttg ctggtatgcc gggtggtgtc cacctggcgc acgcggcccg actcgacttc
     5221 cacgaagtgc aggcgattgg cgctgtcggc gaaggccagc ttcttcgagt ccggcgacca
     5281 caccggcggg aagcgccaca cgctgccgtc gctggtgacg cgtttcggcg cgccgctgcc
     5341 gtcctgcgag cggacgtaga tctcgtactc gccggtggcg tccgagaggt aggaaatcca
     5401 cttgccgtcg ggcgaccacg ccacgctgat ctcgcgggca tccggcgtgt tcgagatgtt
     5461 gcgaatctcg ccatgctgcg cgggcgccgt gaaaatttcc ccgcgcgcgc cgaacaccgc
     5521 gcgctcgccg cccggcgcga tgtcgaagct ctcgatctgc tttgtgacgt tcctgaagcc
     5581 ggccagggtg tgcggcttgt cgccgcgcac cgcgatgtcg agccggcgcg tctcgcccgc
     5641 cgccggatcg tgcagccaga tgtagccgcc gttctcgaac accacgcgct tgcgatccga
     5701 gctcggccac agcacgtcga actcggtgaa gtcggtgacc ttgctgagat tcccgccgtc
     5761 catgtccatg acgaacaggt tcagcgtgaa atcgcgatcg gacacgaaaa acacgcggcc
     5821 gtccacccac atgggctggt ggtcggtgcc gatgaagtcg gtcagctgcc gcgaggtgtt
     5881 gttggccagg tcgtaggtcc agacattctg tgcgcggccg ccgcgatggc gcttccaggt
     5941 gcggaactcg cggtcgatgg gggtgtagac ataggtgttg ccgtcgcccg acagcatgcc
     6001 gccgccggtc tccggcgggc cgagcggacg ctccatgccg ccttcgaagg gcaccagcat
     6061 cggcacgccg tcgcgctcgc cgtagggcgt gcggttggcg cgcaccagga cgtgcttgcc
     6121 atccggcgtc cagtcgagca cgcggtagtc ggtgccgccg cgcggcggca tcgggccgac
     6181 gtcgctgtag aaggtgagct ggcgcggcgt gccgccggcg gacggcatca cccagacctg
     6241 gcgggtgccg gaatactcgg cggaaaaggc gatccaccgc ccgtccggcg agaacttcgg
     6301 atacagctcc tggccctcgt gcgaggtgag gcgctgcgcc acgccgccct gcacgaaagc
     6361 gctgtagatg tcgccgccgt gcacgaaggc gacccggtcg ccgttgatgt cggggaaacg
     6421 cagcaggcgc gtctcggcgg taccggtgga gggggcgatg agcgcaagcg cgatggtcag
     6481 cagtagtacg ggtcgcatgc cggaactccg gaaaaaagtg gggtgattct aaccgcgccg
     6541 ccggggtcgc gggcgtcaga tgatcttttt cttgcggcgg ccgtcgtaga agctctcgat
     6601 cagcaggatc gcggcgccca cggtgagcgc agaatccgcg acgttgaatg ccgggaagaa
     6661 ccagcggtca tagtgcacgc tgatgaagtc gaccacgtga ccgcgaaaca gccggtcgat
     6721 cacgttgccg atcgcgccgc cgacgaccag ggccagcgag gcggccagcc aggcctggcc
     6781 gcggcgcggc atgcgcttca gccagacgca gaccagcgcg gtgacggcca ccgcgatcgc
     6841 gacgaagaac cagcgctgcc agccgccggc cgaggcaagg aaactgaagg cggcaccggt
     6901 attgtgcaag cgggtgatgc cgaataccgg cagcagctgg acgcgatcgt agagttcgaa
     6961 agtcgcgacg atgtacgcct tgctgagctg gtcggccacc acgacgaacg ccgacagcca
     7021 cagccatccc agcgcgccgc gctcccgccc cgccggatac gcctgctcgc tcatgcccag
     7081 cgcctctgct cgccggcgcc gctgacgttc accgcgcaac ggccgcagat ctccgggtgg
     7141 tctggatcca cgccgacgtc cgcacggcgg tgccagcagc gcacgcactt ggcatggtcc
     7201 gtcggcagca cctgggcgta caggcgagcg gcgaagccct ccagctcgat cgcatccgcc
     7261 ggacgcgcat cgagcggctc gacgcgcgcc gtagaggtca gcaacaggaa gcgcaactcg
     7321 tcgcccggac ggcgcagcct ggcggcgaga tcgtccgcgg catagagcac cagctccgca
     7381 tccagcgagg agccgatgcg acctgcgttg cgcagcgcct ccagctcgcg cgcgacgtcg
     7441 tcgcgcaccg cgtgcaccag gtcccagtcg ggatcgtcct cgcgcagcgg cacttcggga
     7501 agctcgtacc agccggacaa cagcaccgac tcggcgcgct caccgggcag gctcgaccag
     7561 atttcctccg cggtgaaggc gagcaccggc gccagccagc gcaccatcgc ctcggccacg
     7621 tgatacatcg cggtctgcgc ggagcggcgc ggctggccgt cggccggggt ggtgtagagc
     7681 cggtccttga tgacgtcgag atagaagccg cccatgtcca gcacacagaa gttgtgcacc
     7741 ttctggtaga tcaggtggaa ctcgtagtcg cgataggcct ggacgacctc ttcctgcagc
     7801 gccgcggtac gcgccaccgc ccagcgatcc agcgcgatca tgtcctccgg cgcgacggcg
     7861 tggctggcgg gatcgaagcc gtccaggtta ccgagcagga agcgcgccgt gttgcgcatg
     7921 cgccggtagg agtcggacat gcgcttgagg atctcctcgg acacgttcat ctcgccgcgg
     7981 tagtcggtcg cagccaccca cagtcgcagc acgtccgcgc cgagcgagtt gagcaccttc
     8041 tgcggcgcga ccacgttgcc gaggctcttc gacatcttgc ggcccttctc gtccacggtg
     8101 aagccgtggg tcaggaccgc gcgatacggc gccgtgccgt gcatcgccac cgcggtcagc
     8161 agcgagctct ggaaccagcc gcggtgctgg tcggagcctt ccagatacag ctccgccggg
     8221 tagcccacct cttcgcgtgt cgcactcagg caatggtgca tcaggccgga atccacccac
     8281 acgtccatga tgtcctgcac tttctcgtag cgcgccgcct cgtcgccgag cagctcggcg
     8341 ggctccagcg accaccaggc gtcgataccc tctttctcga cgcggtctgc cacttcctgc
     8401 agcaggcgca ccgagtcagg gtgcggcgcg ccgctgtcgc ggtccacgaa cagggcgatg
     8461 ggtacgcccc aggtgcgctg ccgggaaacg caccagtcgg gccgcccctc gaccatggcg
     8521 cggatgcggt tctcgcccca gccgggcatc cacttcacct cgccgatggc ctgcagggcg
     8581 cgctcgcgca ggccggcgcg ttccatgccc acgaaccact gcggcgtggc gcggaagatc
     8641 agcggcgtct tgtggcgcca gcaatgcggg tagctgtgca gcatgggctc gtggcgcagc
     8701 agcgcgcccc gtgcctcgag cagttcgacg atcaccgggt tggcatcgaa caccttgagg
     8761 ccggccaccg cctcggtgcc ggtgcggaag cggccgtcgt ccagcaccgg gttgtccacc
     8821 tccagcccgt agcgctggcc gacaatgaaa tcgtcgtggc cgtggccggg tgcggtgtgc
     8881 actgcgccgg tgccggcctc ggtggtgacg tgctcgccga ggatcaccgg cacctcgcgc
     8941 gccaggaaag gatgctgcag cacgacgccc tcgagcgcgg cgccgcggca caccgcgagc
     9001 tcctcgaagt gctcgatgcc ccagcgccgc gtggtcgcgt ccagcaggtc gcgcgccacc
     9061 accagccgct cgcgcccgac cgggccgtcg aagctgatca gcgcgtactc gagctccggg
     9121 tgcagcgcca cggcctggct ggccggcagc gtccagggcg tcgtggtcca gatggggatc
     9181 gacgccgggc cggcgacatc gtcgcgcgcc gcaccgccga aggccgccag cagggcagcc
     9241 tcgtccacca cgcggtagcg cacgtcgatg gccggggatt tcttgtcggc atactcgacc
     9301 tccgcctcgg ccagcgccga gccgcaatcg aggcaccagt gcaccggctt gaagcccttg
     9361 tagacgtggc cgttggccag gatcttcgcg aaggcccgga gctgctccgc ctcgtaggcc
     9421 gggtccatgg tgaggtaggg ccgctcccag tcaccgagga tgccgagccg cctgaagtcc
     9481 cgccgctgcc catccacctg cgagcgcgcg aacgcgcggc aggcgtcgcg gaaggccttc
     9541 gcgtccagct tcgcgccgac cttgccgtgc tcgcgctcga cgaccagctc gatcggcagg
     9601 ccgtggcagt cccagcccgg gatgtagggc gcgtcgtaac cgtccagcgt ccgcgagcgg
     9661 atcacgatgt ccttcagcac cttgttgacg cagtggccga gatgaatgtc gccgttggcg
     9721 tacggggggc cgtcgggcaa caggaaggcg ggacgacctc gagcggccgc gcgcagcttg
     9781 ccgtacaggt tgcgcgcttc ccagtccgcc agcatgtccg gctcgcgctg tgccagcgcc
     9841 gcgcgcatcg ggaagtccgt gcgcggcagg ttcagcgtgt ccttgtaatc agccaccttc
     9901 tcttccttct tcaatagccc gcgtcggcca ggatccgtcg cgccagtttc gcgtcccggt
     9961 gcatctgggc cttgaggccc gcaatgtcgt cgaagcgctg ctcgtcacgc aagcgcgcca
    10021 cgaaatccac cgtgatccgc cgaccgtaca ggtcgccgga gaaatcgaac aggtgcgcct
    10081 cgagcaatgg ctcggtgcca tccaccgtcg gccgggtgcc caggttggcg accgccggcc
    10141 agggccgctc gcccacgccc cggaccagca ccgcgaacac cccggtgacc ggcagcaccc
    10201 ggcggcctgg ccgcaggttg gcggtgggga agcccagcgt ctggcccagg cgccgcccct
    10261 cgatgacccg gccggtcatc ccataatgcc gtcccagcag gcgcgccgcg agccccaggt
    10321 cgccctctgc cagtgccgcc cgcaccgcgg tgctgctcac gcgcaagccg tcgagtacca
    10381 ccgtgggcgt gtcctcgacc tcgaagtagt gctcacggcc agctgcctgc agcgacgcga
    10441 aattgccggc gcggttgcgg ccgaaacgaa aatcgtcgcc gatcaccagg taacgcgtgc
    10501 ccagcccgtc caccaggtac tcgcggatga agtcctcggg ctcgagcgcc gccatgttcg
    10561 catcgaaacg ggcgcaaaac agccggtcca cacccagcgc cttcaggccg acgaatttct
    10621 cgcgaaagcg ggccaggcgc ggcggcgcgg cggcgcccat gaagtactcc cgcggcgtcg
    10681 gctcgaagct catcaccacg gccgccacac cgagggcccg cgccttgccg gtcaagcgct
    10741 ccagcaccgc ccggtggccg agatgcaggc catcgaagtt gccgatggtg accgcgcaac
    10801 cccggtgacc gggacgcagg ttgtgctttc cccgaatgag ttccaagtgc ggattcctgg
    10861 cctcggcgcg
//
LOCUS       DAOMPG010000064        10647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2669_length_10647_cov_5.266144, whole genome shotgun sequence.
ACCESSION   DAOMPG010000064 DAOMPG010000000
VERSION     DAOMPG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2669_length_10647_cov_5.266144"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLS34_05020"
     CDS             <1..235
                     /locus_tag="PLS34_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86866.1"
                     /translation="PCLHADGLSDWWIYAEGDGHRRLIGRQRSVRAVEVLQARSHGFA
                     DLRVVTPDGATTVRYADGGYRMPAEDDPAPGIP"
     gene            complement(249..1274)
                     /locus_tag="PLS34_05025"
     CDS             complement(249..1274)
                     /locus_tag="PLS34_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRP86867.1"
                     /translation="MTHDLNDTLIFVKVVEHGSFVGAARSLRVPKTTVSRKVQELESR
                     LGAQLLHRTTRKLGLTEAGNIYYEHSQRIARDLTEAESAVSQLQAGPRGWLRFTAPYS
                     IGIDKIAPLLGEFHRRHPEVRVEMMLSNEPLDLIASEIDVALRLGELPDSSLVARRLG
                     SLRTQVFASSSYIERHGEPMHPDELQYHRALAMAKHRQGHGYAWTLDDGNGPQQFPIN
                     PILVANDPAALKGALLCGEGLIVGADLMVKPAVEKGEVRRVLAGWVGPVFELNAVFPR
                     GHGQSPKVRAFVDFLLERLNLEIDYMSARCPLEAGRCSRHDDEADAGERDDIAAGKRI
                     LESVTVD"
     gene            1399..1644
                     /locus_tag="PLS34_05030"
     CDS             1399..1644
                     /locus_tag="PLS34_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86868.1"
                     /translation="MPAAPRRQLSRASLLLLLAVDLIALSALVMNQWLGLARGWSWLL
                     GTVLGLALLPVLLRLVDRVLAAARAARNIPIAGGTFP"
     gene            1788..2987
                     /locus_tag="PLS34_05035"
     CDS             1788..2987
                     /locus_tag="PLS34_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP86869.1"
                     /translation="MNIADPTARCATRAARLVPVLLAAALAVACSSNASPGDGMPPPP
                     EVSVAEVVARPVTQWDDFTGRIAAVETVDLRPRVSGYVQRVAYEEGEAVRKGDLLFEI
                     DPRPYQAALDRAQADLERARSEARLANAQNVRAKALVEVRAISREAFETRNAATAQGN
                     AAVRAAEAAVAAARLDLQFTRVRSPIDGRAGRALVTTGNLAQADATLLTTVVSQDPVH
                     VYFETDEQTWLRYSQLARDGERAASDNAVRVGLAGEDGHPHAGVVDFTDNRVDPATGT
                     IRARAVLRNPDGVFTPGLFARVQLEGSGEFDALLVDDKAVLTDQDRKYVYVLGEDDTA
                     QRRDVVLGRVIDGLRVVASGLAPGDAVIVGGVQKVFMPGMPVAPKTVAMRATDNSRTA
                     AAAAAAP"
     gene            3354..6515
                     /locus_tag="PLS34_05040"
     CDS             3354..6515
                     /locus_tag="PLS34_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRP86870.1"
                     /translation="MDFSRFFIDRPIFAAVLSIVIFAAGLISIPLLPISEYPEVVPPS
                     VVVRTVYPGANPKVIAETVAAPLEESIRGVEGLMYMKSVASSDGSSTTTVTFQPDVDA
                     DEATVRVQNRVSQALTRLPEDVRRQGVTTQKQSPVFLMVVHLTSPDGKYDTLYLRNYL
                     RLHVKDELASIPGVGDAQAFGGGDYAMRIWLNPHEVAARGMTAGDVVRAVREQNVQVS
                     AGQLGAEPMPGGSEFLVPINARGRLSSPEEFGDIVLKTGDGGELVRLADVARVELAAG
                     DYSMRARLDGENAAAIGIFQAPGANALQIRDEVIARMDALSERFPPGVEYRSIYDTTM
                     FVRDSIKSVITTLLEATGLVVLVVILFLQTWRASIIPLLAVPVSIVGTFAALYLLGFS
                     INTLTLFGLVLAIGIVVDDAIVVVENVERHIEEGATPLAAAHLAMKEVSGPIIAIALV
                     LCAVFVPMAFLSGVTGQFYKQFAVTIAISTVISAINSLTLSPALAARLLKPHDAPKDA
                     PSRLIDRLFGWIFRPFNRFFGTSAERYQRGVSKALGRRGAVFAVYAVLLAATALMFQV
                     VPRGFIPVQDKSYLIAGVKMPEGSSIERTDAALKKIGEIALSIDGTQNDVAFPGFNPL
                     QFINTPNYGVTFINLKPFGERSRSATEINAELSAKIGGIQEGFAFSLLPPPIDGLGTG
                     SGYSAFVQDRGNLGYGALQSTVQAFQGAGAQEPGLGFINSSYQANVPQLDAEVDRVKA
                     KAQGVPLTELFDTLQTYLGSAYVNDFNLFGRTWQVIAQADGPYRDSVEDIASLRTRNL
                     DGQMVPIGSMVTVHESFGPDPVIRYNGYPAADLMGESDPRLLSSAQAMDRIEQLAAQV
                     LPNGMQLEWTDLSYQQKTQGKAALVIFPLAVLLAFLVLAALYESWTLPLAVILIVPMC
                     MLSALLGVWLTGDNNVFVQVGLVVLMGLACKNAILIVAFARELELQGKGIVEAALQAC
                     RLRLRPIVMTSIAFIAGTVPLVLSHGAGAEVRQATGITVFSGMIGVTLFGLFLTPVFY
                     VALRKLANRPLVSHAPDAEAAQA"
     gene            6625..7371
                     /locus_tag="PLS34_05045"
     CDS             6625..7371
                     /locus_tag="PLS34_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP86871.1"
                     /translation="MSTPSRIALVTGATRGIGLETVRQLAAEGVHTLLAGRDPARTAA
                     AAAVLQAEGLPVESITLDVTDAASILAAAARVRERHGRLDILANNAGIFLDDPDKAPS
                     EQSLAAWRDTFETNLFGLVAVTQAFLTLLRESAAGRIVNVSSSLASLVLNNDAGYEFY
                     DFKMPAYNVSKSAVNAWTVQLAWELKGSGVKVNTIHPGYVKTDMTMAHGEQGGDLEVS
                     DGARTSVRLALIDDDGPSGGFFHRDEVLPW"
     gene            7368..7574
                     /locus_tag="PLS34_05050"
     CDS             7368..7574
                     /locus_tag="PLS34_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86872.1"
                     /translation="MTRFATTALAAALVTACAAGPDLVRPQVAVSERFGAAARYKALA
                     DGWPRLLPEREKIAAAGIGAGSGP"
     gene            7571..7891
                     /locus_tag="PLS34_05055"
     CDS             7571..7891
                     /locus_tag="PLS34_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRP86873.1"
                     /translation="MNAWWLVAAAGAIEVVMALALKASHGLSRPVPAAVGLLAAVASL
                     PLLTMAMRQLPAGTAYAVWTGIGGVGVALFGILAFGDSASPSRLLCIVLIVAGIAGLR
                     LLEA"
     gene            7933..8979
                     /locus_tag="PLS34_05060"
     CDS             7933..8979
                     /locus_tag="PLS34_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRP86874.1"
                     /translation="MDYRFLGNSGFKVPALGFGAGTFGGKGALFSAWGNTDVAQARRL
                     VDICLEAGANLFDTADVYSDGASEEILGAAVEGRRDAVILSTKLTLRAGDGPNDVGAS
                     RHHLVAGVDRALRRLRSDWIDLLQLHHYDARTPVEDVMSTLDDLVRAGKLRYIGASNF
                     SGWQLMKSQAAAARQGRARFVAHQVYYSLIGRDYEWELMPLAQDQGIGAVVWSPLGWG
                     RLTGKVRRGQPLPPGSRLHDTAMFAPPVADERLYRVVDALDLIAAETGRTVPQVALNW
                     LLQRPTVSTVLVGARDEAQLRQNLGAVGWNLSPAQVAQLDEASRVPTPYPYYPYWNGQ
                     FSERTPPPVLAHRG"
     gene            9062..10117
                     /locus_tag="PLS34_05065"
     CDS             9062..10117
                     /locus_tag="PLS34_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRP86875.1"
                     /translation="MPQAETANRRIVLASRPVGLPGITDFRLEHAPIPRPRDGEVLLR
                     TLYLSLDPYMRGLMDEVGPVYARRVELGQPMAGATVSRVVESRHPAYRVGQRVLASAG
                     WQDYAVSDGSGLSPVDTIEPVSWALGGLGMPGFTAHVGLLDIGQPRSGETVVVAAATG
                     AVGSVVGQIARIKGARVVGIAGGAEKCRHAIEQLGFDACVDHRAPDFVQRLAEACPGG
                     IDVYFENVGGEVFDAVLPLLNIGARVPVCGIIAHYNAHQPPPGPDRLPLLQATLLQKR
                     IRMQGFIILDHYGERFDAFRRDMTGWLEAGRVHLREDRVDGLENAPTAFIGLLQGRNF
                     GKLVVRVDSGQGTGTNQ"
     gene            10114..10464
                     /locus_tag="PLS34_05070"
     CDS             10114..10464
                     /locus_tag="PLS34_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRP86876.1"
                     /translation="MSAVNPESKLATFSGHWQPRTISSFNGNDVMVVKALGAFTWHRH
                     DDTDDFFYVLKGRLRIEMRDHAVDLGPGDLYIVPRGVEHRPVAEEEVHLLLIEPTGTP
                     NTGDAASAAVRRVI"
     gene            10531..>10647
                     /locus_tag="PLS34_05075"
     CDS             10531..>10647
                     /locus_tag="PLS34_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRP86877.1"
                     /translation="MGKSDDTRNGVPRAQAEDAVRTLLRWSGEDPAREGLLDT"
BASE COUNT         1573 a         3753 c         3666 g         1655 t
ORIGIN      
        1 cccctgcctg cacgccgacg gcctgtcgga ctggtggatc tacgccgagg gcgacggcca
       61 tcgccgcctg atcggcaggc agcgttcggt gcgcgcggtc gaggtgctgc aggcgcgcag
      121 ccatggtttc gccgatctgc gcgtggtcac gcccgatggc gcgaccaccg tgcgctatgc
      181 cgatggcggc taccggatgc cggcggaaga cgatcccgca cccggcatcc catgagcgcg
      241 gcacgcactc agtcgacggt gaccgactcc aggatgcgct tgccggcggc gatgtcgtcg
      301 cgctccccgg cgtcggcctc gtcgtcgtgg cggctgcacc tgcccgcctc cagcggacag
      361 cgcgccgaca tgtagtcgat ctcgaggttc agccgctcga gcaggaaatc cacgaacgcg
      421 cgcaccttcg gcgactgccc gtggccgcgc gggaacaccg cgttgagttc gaacaccggg
      481 ccgacccagc cggcgagcac gcgccgtact tcgcccttct cgaccgcggg cttgaccatc
      541 aggtcggcgc cgacgatcag cccttcgccg cacagcaggg cacccttgag cgcggccggg
      601 tcgttggcga ccaggatcgg gttgatcggg aactgctgcg ggccgttgcc gtcgtcgagc
      661 gtccacgcat agccgtggcc ctggcggtgc ttggccatcg ccagcgcacg gtggtactgc
      721 agctcgtccg gatgcatcgg ttcgccgtgg cgctcgatgt agctgctgct ggcgaacacc
      781 tgggtgcgca ggctgccgag gcggcgcgcg accaggctcg aatcgggcag ctcgcccagg
      841 cgcagggcga catcgatctc gctggcgatc aggtcgagcg gttcgttcga cagcatcatc
      901 tccacgcgca cttccgggtg gcgcctgtgg aactcgccca gcagcggcgc gatcttgtcg
      961 atgccgatcg agtacggcgc ggtgaagcgc agccagccgc gcgggccggc ctgcagctgg
     1021 ctgaccgcgc tttcggcctc ggtcaggtcg cgcgcgatgc gctgcgagtg ctcgtagtag
     1081 atgttgccgg cctcggtcag gccgagcttg cgcgtggtgc ggtggagcag ttgcgcgccc
     1141 aggcgcgatt cgagttcctg caccttgcgg ctgaccgtgg tcttgggcac gcgcagcgag
     1201 cgcgccgcgc cgacgaagct gccgtgctcg accaccttga cgaagatcag ggtgtcgttg
     1261 agatcgtgcg tcatcgccgt ctcctgcatc cgattcgtgg attaacccac atattggaca
     1321 attattccct caatcgggga ctaatcaagt cctgatttga ggcgcaggat aggcgccctc
     1381 ccccgatgga ccgtcgtcat gcctgccgcc ccgcgccgcc agctgtcccg cgccagcctc
     1441 ctgctgctgc tggccgtgga tctgatcgcc ctgagcgcac tggttatgaa tcagtggctt
     1501 ggccttgctc gcggctggag ctggctgctc ggcacggtgc ttggcctggc cctgctgccg
     1561 gtgctgctgc gcctggtcga ccgggtcctc gcggcagcgc gcgcggcacg gaatatcccg
     1621 atcgcgggag gaacattccc gtgaccggga caatcggtcc cagggcaggg cgccatcgcc
     1681 ccgtcgtgcc cgtcgtcgcc gtcgccgtgt ccatgacctc ccgcgtcatc gccagcgcac
     1741 cggcgcccgc gccatcgtct tccccacttc gccaggatgc cttccccatg aacatcgccg
     1801 atccgaccgc acgctgcgcc acccgcgccg cgcgcctggt ccccgtgctg ctcgccgccg
     1861 cgctcgcggt cgcctgcagc agcaacgcct cccccggcga cggcatgccg ccgccgcccg
     1921 aagtcagcgt cgccgaggtg gtggcgcgcc cggtgaccca gtgggacgac ttcaccggcc
     1981 gcatcgccgc ggtcgagacc gtcgacctgc gcccgcgcgt gagcggctac gtgcagcgcg
     2041 tggcctatga agaaggcgag gccgtgcgca agggcgacct gctgttcgag atcgatccac
     2101 gcccctacca ggctgcgctg gatcgtgcgc aggccgacct tgaacgcgcg cgcagcgagg
     2161 ccaggctggc caacgcgcag aacgtgcgcg ccaaggcgct ggtcgaggtg cgcgcgatct
     2221 cgcgcgaggc gttcgagaca cgcaacgccg ccaccgcgca gggcaatgcc gcggtccgcg
     2281 ccgccgaggc cgcggttgcc gcggcgcggc tggacctgca gttcacccgg gtgcgttcgc
     2341 cgatcgacgg ccgcgccggg cgcgcgctgg tgacgaccgg caacctcgcc caggccgacg
     2401 ccacgctgct gaccacggtg gtctcgcagg atccggtgca cgtctacttc gaaaccgacg
     2461 agcagacctg gctgcgctac agccagctcg cgcgcgacgg cgagcgtgcc gccagcgaca
     2521 acgccgtgcg cgtcggcctc gccggcgagg acggccaccc gcacgcgggc gtggtcgact
     2581 tcaccgacaa ccgcgtcgat ccggccaccg gcaccatccg cgcgcgcgcg gtgctgcgca
     2641 atcccgacgg cgtgttcacg ccgggcctgt tcgcgcgggt gcagctcgag ggcagtggcg
     2701 agttcgacgc gctgctggtc gacgacaagg ccgtgctcac cgaccaggac cgcaagtacg
     2761 tctacgtgct aggcgaggac gacaccgcac agcgcaggga cgtggtgctc ggtcgcgtga
     2821 tcgacggcct gcgcgtggtc gcgtcgggcc tggcgccggg cgacgcggtg atcgtcggcg
     2881 gcgtgcagaa ggtgttcatg ccgggcatgc cggtggcacc gaagaccgtg gcgatgcgcg
     2941 cgacggacaa cagccgcact gccgcagcgg ccgccgcggc gccgtagcga tgcttcactc
     3001 cctccctttc gcgtagcgaa gggtgaggga ggcctgcctg cacggcattg ccgtgcaggc
     3061 cgcccgaacg accggcgcgc tgcgccgggc cggacgggtc ggagacgggt gcttttgctg
     3121 tccgcagttg cagtccaggc aatacgccaa cagcaccccc tcccaacttc tccctgcccg
     3181 ctgcgcggtc aaggggaggc gttctgctcc ccctcgggcc gctccctccg gcccgtccgg
     3241 ccggcggcct tccatgcctg cttccccagg cagggaaggt cgccggaccg gatcttcttc
     3301 cccacgtcgc gccgcgcgcg gcgaccgcgg ccgttgccgc aaggacaacc cccatggact
     3361 tctccagatt cttcatcgac cgcccgatct tcgccgccgt gctgtcgatc gtgatcttcg
     3421 ccgccggcct gatctcgatc ccgctgctgc cgatctccga atacccggaa gtggtgccgc
     3481 catcggtggt ggtgcgcacg gtgtatccgg gcgccaaccc gaaggtgatc gccgagaccg
     3541 tcgccgcgcc gctggaggaa tcgatccgcg gcgtggaagg gctgatgtac atgaagtcgg
     3601 tcgccagctc cgacggctcg tcgaccacca ccgtcacctt ccagcccgac gtcgacgccg
     3661 acgaagccac ggtgcgcgtg cagaaccgcg tcagccaggc cctgacgcgc ctgcccgagg
     3721 acgtgcgccg ccagggcgtg accacgcaga agcagtcgcc ggtgttcctg atggtggtgc
     3781 acctgacttc gcccgacggc aagtacgaca ccctgtacct gcgcaactac ctgcgcctgc
     3841 acgtcaagga cgagctggcg agcattcccg gcgtcggcga cgcgcaggcc ttcggcggcg
     3901 gcgactacgc catgcgcatc tggctcaacc cgcacgaggt ggccgcgcgc ggcatgaccg
     3961 ccggcgacgt ggtgcgcgcg gtgcgcgagc agaacgtgca ggtctcggcc ggccagctcg
     4021 gcgccgagcc gatgcccggc ggcagcgagt tcctggtccc gatcaacgca cgcggccggc
     4081 tgtcgagtcc cgaggagttc ggcgacatcg tgctcaagac cggtgacggc ggcgagctgg
     4141 tgcggctggc cgacgtggca cgcgtggagc tcgcggcggg cgactacagc atgcgcgcgc
     4201 gcctggacgg cgagaacgcc gccgccatcg gcatcttcca ggcgccgggc gcgaacgcac
     4261 tgcagatccg cgacgaggtg atcgcgcgca tggacgcgct gtcagaacgc tttccgcctg
     4321 gcgtggaata ccgctcgatc tacgacacca ccatgttcgt gcgcgactcg atcaagtcgg
     4381 tgatcaccac cctgctcgaa gcgaccgggc tggtggtgct ggtggtgatc ctgttcctgc
     4441 agacctggcg tgcctcgatc atcccgctgc tggcggtgcc ggtgtcgatc gtcggcacct
     4501 tcgccgccct gtacctgctc ggtttctcga tcaacaccct gaccctgttc ggcctggtgc
     4561 tggcgatcgg catcgtggtc gacgatgcga tcgtggtggt ggagaacgtc gagcgccaca
     4621 tcgaggaggg cgcgacgccg ctggcggccg cgcacctggc gatgaaggaa gtctccgggc
     4681 cgatcatcgc gatcgcgctg gtgctgtgcg cggtgttcgt gccgatggcc ttcctgagcg
     4741 gcgttaccgg ccagttctac aagcagttcg cggtgaccat cgcgatctcc acggtgatct
     4801 cggcgatcaa ctcgctgacc ctgtcgccgg cgctggcggc gcgcctgctc aagccgcacg
     4861 acgcgccgaa ggatgcgccg tcgcggctga tcgatcgcct gttcggctgg atcttccggc
     4921 cgttcaaccg cttcttcggc accagcgccg agcgctacca gcgcggcgtg agcaaggcgc
     4981 tgggccggcg cggcgcggtg ttcgcggtgt acgcggtgct gctggccgcc accgcgctga
     5041 tgttccaggt ggtgccgcgc ggcttcatcc cggtgcagga caagtcctac ctgatcgccg
     5101 gcgtcaagat gccggaaggt tcgtcgatcg agcgtacgga cgctgcgctg aagaagatcg
     5161 gggaaatcgc gctgtcgatc gacggcaccc agaacgatgt cgccttcccc ggcttcaacc
     5221 cgctgcagtt catcaacacg cccaactacg gggtgacctt catcaacctc aaaccgttcg
     5281 gcgagcgcag ccgcagcgcg accgagatca atgccgaact gtcggcgaag atcggcggca
     5341 tccaggaggg tttcgcgttc tcgctgctgc cgccgccgat cgacggcctg ggcaccggtt
     5401 cgggctactc ggccttcgtc caggaccgcg gcaacctcgg ctacggcgcg ctgcagagca
     5461 cggtccaggc cttccagggc gcgggcgcgc aggaaccggg gctgggcttc atcaactcca
     5521 gctaccaggc caacgtgccg cagctcgacg ccgaggtcga ccgggtcaag gccaaggccc
     5581 agggcgtgcc gctgaccgag ctgttcgaca ccctgcagac ctacctcggc tcggcctatg
     5641 tcaacgactt caacctgttc ggccgcacct ggcaggtgat cgcgcaggcc gacgggccct
     5701 accgcgacag cgtcgaggac atcgcaagct tgcgcacgcg caacctcgac ggccagatgg
     5761 tgccgatcgg cagcatggtg acggtgcacg agagcttcgg cccggacccg gtgatccgct
     5821 acaacggcta cccggccgcc gacctgatgg gtgaatccga tccgcggctg ctgtcctcgg
     5881 cgcaggcgat ggacaggatc gagcagctgg ccgcgcaggt gctgcccaac ggcatgcagc
     5941 tggagtggac cgacctgtcc taccagcaga agacgcaagg caaggccgcg ctggtcatct
     6001 tcccgctggc ggtgctgctc gccttcctgg tgctggccgc gctgtacgag agctggaccc
     6061 tgccgctggc ggtgatcctg atcgtgccga tgtgcatgct ctcggccctg ctcggggtgt
     6121 ggctgaccgg cgacaacaat gtcttcgtgc aggtcggcct ggtggtgctg atggggctgg
     6181 cgtgcaagaa cgcgatcctg atcgtggcgt tcgcgcgcga gctggagctg cagggcaagg
     6241 gcatcgtcga agccgcgctg caggcctgcc gcctgcgcct gcgcccgatc gtgatgacct
     6301 cgatcgcctt catcgccggc accgtgccgc tggtgctctc gcacggcgcc ggcgcggaag
     6361 tgcggcaggc gaccggcatc accgtgttct cgggaatgat cggcgtgacc ctgttcggcc
     6421 tgttcctgac cccggtgttc tacgtcgcgc tgcgcaagct ggcgaaccgg ccgctggtgt
     6481 cgcacgcgcc cgacgccgag gcggcgcagg catgacgcgc ctgccaggtc cgaaaccggc
     6541 gagcgtcacc gcatccacca aaccacactc gcctcggcgc acatgatcgc gcgcaccttc
     6601 ccccgactcc gagagacaga ccccatgagc acaccatccc gcattgccct cgtcaccggt
     6661 gccacccgcg gcatcggcct ggagaccgtc cgccagctcg ccgccgaagg cgtgcatacc
     6721 ctgctcgccg ggcgcgatcc cgcgcgtacc gccgcggccg ccgccgtgct gcaggccgag
     6781 ggcctgccgg tcgagtcgat cacgctcgac gtgaccgacg ccgccagcat cctggccgcc
     6841 gccgcgaggg tgcgcgaacg ccatggccgg ctcgacatcc tggccaacaa cgccggcatc
     6901 ttcctcgatg acccggacaa ggcaccgtcg gagcagtcgc tcgcagcctg gcgcgacacc
     6961 ttcgagacca acctcttcgg cctggtcgcg gtgacccagg ccttcctgac gctgctgcgc
     7021 gaatccgccg ccggccgcat cgtcaatgtg tcgagctcgc tggcctcgct ggtcctgaac
     7081 aacgatgccg ggtacgagtt ctacgacttc aagatgccgg cctacaacgt ctccaagagc
     7141 gcggtgaacg cctggacggt gcagctggcc tgggagctca agggcagcgg cgtcaaggtc
     7201 aacaccatcc atccgggcta tgtgaagacc gacatgacca tggcccatgg cgagcagggc
     7261 ggcgacctgg aggtctccga tggcgcgcgc accagcgtgc gcctggcgct gatcgacgac
     7321 gacggcccca gcggcggctt cttccaccgc gacgaggtgc tgccatggtg acccgattcg
     7381 cgaccaccgc gctcgccgcc gcgctggtga cagcttgcgc ggccggtccg gacctcgtgc
     7441 gcccgcaggt ggcggtgtcc gagcgcttcg gcgcggccgc gcggtacaag gcgctggccg
     7501 acggctggcc gcggctgttg ccggagcgcg agaagatcgc cgcggcaggc atcggcgcag
     7561 gatcgggccc gtgaacgcgt ggtggctggt cgctgccgcg ggcgcgatcg aggtggtcat
     7621 ggcgctggcg ctgaaggcct cgcacggatt gagccggccg gtcccggcgg cggtgggcct
     7681 gcttgcggcg gtggcgagcc tgccgctgct gaccatggcc atgcgccagc ttcccgccgg
     7741 caccgcttat gcggtctgga ccggcatcgg cggcgtcggc gtggcgctgt tcggcatcct
     7801 cgccttcggc gacagcgcgt cgccgtcgcg cctgctgtgc atcgtcctga tcgtcgccgg
     7861 catcgccggg ctgcgcctgc tggaggcgtg atcgcccatc gcatcgcgcg aaacatcaca
     7921 cggagcaaca ccatggacta ccgatttctc ggcaattccg gcttcaaggt cccggcactg
     7981 ggcttcggcg ccggcacctt cggcggcaag ggcgcgctgt tcagtgcctg gggcaacacc
     8041 gacgtcgcgc aggcgcgcag gctggtcgac atctgcctgg aggccggcgc caacctgttc
     8101 gacactgcgg acgtctattc cgacggcgct tcggaagaaa tcctcggtgc ggcagtcgaa
     8161 ggccgccgcg atgcggtgat cctctcgacc aagctcacgc tacgcgcggg cgacgggccc
     8221 aacgacgtcg gcgcctcgcg ccaccatctc gtcgccggcg tcgacagggc gctgcgccgc
     8281 ctgcgcagcg actggatcga cctgctgcag ctgcatcact acgacgcccg cacgccggtg
     8341 gaggacgtga tgtccacgct cgatgacctg gtgcgcgccg gcaagctgcg ctacatcggc
     8401 gcctccaact tctccggctg gcagctgatg aagtcgcagg cggccgcggc gcggcaaggt
     8461 cgcgcgcgtt tcgtcgcgca ccaggtctac tactcactga tcgggcgcga ctacgaatgg
     8521 gagctgatgc cgctggcgca ggaccagggc atcggcgcgg tggtctggag cccgcttggc
     8581 tggggccggc tgaccgggaa ggttcgtcgc ggccagccgc tgccgcccgg cagccgcctg
     8641 cacgacacgg cgatgttcgc gccgccggtc gctgacgaac gcttgtaccg cgtggtcgat
     8701 gcactcgact tgatcgccgc agaaaccggc aggaccgtgc cgcaggtcgc gctcaactgg
     8761 ctgctgcagc gtccgaccgt gtccaccgtg ctggtcggcg cgcgcgacga ggcgcagctg
     8821 cggcagaacc tgggcgccgt cggctggaac ctctcgcccg cacaggtcgc gcagctcgac
     8881 gaagccagtc gggtgccgac accgtatccc tactatccct actggaacgg gcagttcagc
     8941 gagcggacgc cgccgccggt gcttgcgcat cgcgggtagt acgatgattg tcgccggcgc
     9001 gcgagcgact ggcgggatgg cgacgaccgg ttccacgcaa gccgatccag gaaacgaaga
     9061 catgccccag gcagagaccg ccaaccgccg catcgtgctg gccagccgtc ccgtcggctt
     9121 gcccgggatc accgacttcc ggctcgaaca cgcgccgatt ccgcggccgc gcgacggcga
     9181 agtgctgttg cgcacgctgt acctgtcgct cgatccgtac atgcgcggcc tgatggatga
     9241 ggtcgggccg gtctacgcgc ggagagtgga gctcggccag ccgatggcgg gtgcgaccgt
     9301 cagccgggtg gtggagtcgc gccatcccgc gtatcgcgtg ggccagcgcg tcctcgccag
     9361 cgccggatgg caggactacg cggtctccga cggcagcggc ctgtccccgg tcgacaccat
     9421 cgagccggtc tcatgggccc tgggcggact cggcatgccc gggttcacgg cgcatgtcgg
     9481 cttgctcgac atcggccaac cccggtcggg cgaaacggtg gtcgtggccg ctgccaccgg
     9541 tgccgtcggc tcggtggtcg gacagatcgc gcggatcaag ggcgcgcgcg tggtcgggat
     9601 cgccggtggc gcagagaagt gccgccacgc gatagaacag ctgggattcg atgcctgcgt
     9661 ggaccaccgc gcgccggact tcgtgcaacg cctcgccgaa gcctgcccgg gtgggatcga
     9721 cgtgtacttc gagaatgtcg gcggcgaggt gttcgatgcc gtgttgccgc tgctcaacat
     9781 cggcgcgcgc gtgccggtgt gcgggatcat cgcccattac aacgcgcatc agccaccgcc
     9841 cggaccggac cgcctgccgc tgctgcaggc cacgctgctg cagaagcgca tccgcatgca
     9901 gggcttcatc atcctcgacc actacggcga acgtttcgac gcgttccgac gcgacatgac
     9961 cggctggctc gaggcaggac gggtccacct gcgcgaggac cgggtggatg gcctggagaa
    10021 cgccccgacg gcgttcatcg gcctgctgca gggacgcaac ttcggcaagc tggtggtgcg
    10081 ggtcgacagc gggcagggca cgggaacgaa ccaatgagcg ccgtcaatcc tgaatcgaag
    10141 ctggccacct tctccgggca ctggcagccg cgaacgatct ccagcttcaa tggaaacgac
    10201 gtcatggtcg tcaaggcgct gggtgcgttc acgtggcaca ggcacgatga caccgacgac
    10261 ttcttctacg tgctgaaggg caggttgagg atcgagatga gggaccatgc ggtcgacctt
    10321 ggaccgggcg acctgtatat cgtcccgcgc ggcgtcgagc atcgcccggt tgccgaggaa
    10381 gaggtgcacc tgctcctgat cgaaccgaca ggcacgccca acacaggcga cgccgccagc
    10441 gccgcggttc gcagggtcat ctagtcgggc gcgatgccgt gcggccgcgc taccatgtgc
    10501 gcccgagccg tgcagcgacg aggagccgcc atgggcaaat ccgacgacac ccgcaacggc
    10561 gtcccgcgcg cacaggccga ggacgcggtg cgtaccctgc tgcgctggtc cggcgaggat
    10621 ccggcgcgcg agggcctgct cgacacg
//
LOCUS       DAOMPG010000065        10612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2676_length_10612_cov_4.419153, whole genome shotgun sequence.
ACCESSION   DAOMPG010000065 DAOMPG010000000
VERSION     DAOMPG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_10612_cov_4.419153"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1898)
                     /locus_tag="PLS34_05080"
     CDS             complement(117..1898)
                     /locus_tag="PLS34_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86878.1"
                     /translation="MRTTLSIAALLAVLLLTLLALPGPAPTASGDAAAGATVTAITDV
                     RLFDGEQVTEHATLLLADGLVREAGAGLAVPAGAEVIDGRGKTALPGLIDAHVHVFGP
                     AREDALRFGVTTLLDMFRPPLDAAQIRSERESTAPTPRADLFSAGYLATAPGGHGTQY
                     GFPVPTPTTADADAWVAARLAEGSDYIKIVIEDGSGWGGELPTLAPDTVRALVSAAHA
                     RGVLAVAHVSTHAAARLAIEAGADGLMHLFVDRPVDAPFIEAARAAGAWVVPTATVLA
                     SSHGHSAAEALLEHPALGARLSPAQAQSLGQTFPGGEARRARWPMIMAGVRALQEAGV
                     PLLAGSDAPNPGTAQGASLHHELALLVAAGLAPVDALRAATSVPARAFALAGRGCLRT
                     GCRADLLLVDGNPLADIDATTRIAGVWKNGHAVSLERAVTRAPAAAQGPAGEAGAPLD
                     LVGQHARWLAASDQYQQGRSTAAIDTAAAPGALVVSGVLRAGFAFPYAGAMWNTSEVP
                     MQAADLSDRRTLEIDLEGADGYRVMFFSGEPGAQPTWRDLEGDGALRLELAELDGVDL
                     ARLRAIGVFVSGAPGDFEFTIRAARLH"
     gene            complement(1909..2643)
                     /locus_tag="PLS34_05085"
     CDS             complement(1909..2643)
                     /locus_tag="PLS34_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP86879.1"
                     /translation="MLANPAVRLVEAQWLNLVRTPAYSLPTLLFPVMFYAFFGLVMLP
                     GQSLSLLCTFATFGVMGAALFSFGVGIALERAQGWLLLLRASPAPVYAVIIGKAFGAV
                     LFGLVIVVMMSALAAGFGGVRLHTAQWLGLAGVLVAGTLPFCLLGLALGLWLDPRAAP
                     AVVNIIYLPLAILSGLWIPATQFPPGLQALAGWLPPYHLAALALDVTGARSADWGAAL
                     ANLGVFTLLCGAATVIGWRRLDGSRR"
     gene            complement(2643..3350)
                     /locus_tag="PLS34_05090"
     CDS             complement(2643..3350)
                     /locus_tag="PLS34_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP86880.1"
                     /translation="MAHPIIATCSNVRHAYGRTVALDGVDLALPQGQVTALLGPNGAG
                     KTTLIHLLLGLLPVQSGVIRLFDGLDPADPAIRRRIGVMLQSSGVQGNLTVLELLRLF
                     ASFYGAPGDFDALLEEAGLQGLEQRRFERLSGGQRQRVLFALAAVGRPDWLILDEPTT
                     GLDPAARRQAWRAIEARRAAGASILLCTHFMDEARRLADRVVVMDRGRILHDGPLAEA
                     GEDLESTFFALTEREVA"
     gene            3519..3968
                     /locus_tag="PLS34_05095"
     CDS             3519..3968
                     /locus_tag="PLS34_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86881.1"
                     /translation="MKDQVIRDAPGKTPVHLWIVGVLALLWNAVGAFDYAATQFRFEA
                     YMGQFTPEQLDYFYGIPAWAVAAWAVAVWFAVFGSLALLLRRRAAYTLFLVSLAAMVT
                     STVHSFVLSEGAAIMGSEGVVFSGLIVVIGVLLPWYSKIMADRGVLR"
     gene            complement(4030..5133)
                     /locus_tag="PLS34_05100"
     CDS             complement(4030..5133)
                     /locus_tag="PLS34_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRP86882.1"
                     /translation="MAATPDPLTAPPPPSAFKLRPYLKLMADKSASDLFFTPMAPIKI
                     KIEGQIVPVGKEVMSVEVVNQIAMSIMTEAQKRVLEREHELDFAISETGLGRFRANVF
                     RQRGNIAIVLRYITLEMPKLDDLGLPKVLKDLALRKRGLILMVGATGSGKSTTLAAML
                     NHRNELLSDHIITIEDPIEFLHPNKKSIINQRELGWDTHSYARALKSAMRAAPDVVLI
                     GEIRDQETMEAAITLAGTGHLAIATLHANNAAETLDRIINMFPQSQHRQVLMDMSHYL
                     SAIIAQRLIIGTDGKRVAAVEVLLNTPHIAELISKGDIGGIKQAIRETSQGDMQSFDS
                     SLYKLFKEGRIDLERALAYADSSVDLEARINFG"
     gene            complement(5117..6286)
                     /locus_tag="PLS34_05105"
     CDS             complement(5117..6286)
                     /locus_tag="PLS34_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRP86883.1"
                     /translation="MERDQAIRFIHELLRKMVEVKGSDLFLTAGFPPAIKVDGRVKPV
                     TDQPLTPQQSATLVRSIMNDRQTKEYDATKECNFAIAPPRIGRFRVSAFVQQGHSGAV
                     MRTINTTIPTFEELELPPVLKDVAMTKRGLVLIVGGTGSGKSTTLAAMLGYRNENSKG
                     HIITIEDPVEYVHTHKSCVVTQREIGMDTESWGIALKNTLRQAPDVILIGEIRDRDTM
                     EYGIQFAETGHLCLATLHANSANQALDRVINFFPEDKRDQLLMDMSLNIKGIIAQRLL
                     PRAGGVGRIAAMEVMLASPLISDLIFKGEVHQLKEVMARSNRLGMKTFDQALFELFED
                     GKISYEDALRNADSRNELRLRIKLESKRDFKAADDGSDSLRLADEQKSQTYGRDA"
     gene            complement(6286..7326)
                     /locus_tag="PLS34_05110"
     CDS             complement(6286..7326)
                     /locus_tag="PLS34_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRP86884.1"
                     /translation="MAVDIEQLLRFSVKNGASDLHLSSGVPPIIRIDGDVKRINMPAL
                     THKEVHSMVYDIMNDKQRKDYEEFFETDFSFEIPELARFRVNAFNQHRGAGAVFRTIP
                     SKIMTLDDLNAPKIFKDISMYPRGIVLVTGPTGSGKSTTLAAMIDHVNNNRPDHILTI
                     EDPIEFVHESKKSLINQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDRVVDVFPAGEKEMVRSMLSESLRAVISQTLLKKIGG
                     GRVAAHEIMIGTAAVRNLIREGKIAQMYSAIQTGQAIGMQTLDQCLQDLLRKGLVSRE
                     EARYKAQNKEAF"
     gene            7416..8138
                     /locus_tag="PLS34_05115"
     CDS             7416..8138
                     /locus_tag="PLS34_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRP86885.1"
                     /translation="MTSVSESTVAERLAAVRARVAAAARAAGRDPAGICILGVSKRQP
                     LAAVRAALAAGLEDFGENFVQEALGKMDALGPGRARWHFIGALQSNKTAAVAERFDWV
                     HSVDRDKLAERLSRQRPAGLPPLNVCVQVHIGDEASKSGVPPEQAEALARRVAGLPGL
                     RLRGLMAVPPPEADPARQRAWCRRLAELFAALKARGLALDTLSMGMSDDLEAAVAEGA
                     TVVRIGTAIFGPRIGDIPQIQQ"
     gene            8179..9006
                     /gene="proC"
                     /locus_tag="PLS34_05120"
     CDS             8179..9006
                     /gene="proC"
                     /locus_tag="PLS34_05120"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRP86886.1"
                     /translation="MRQTSIAFIGGGNMARSLVGGLIADGVGAAQLQVAEPDPERRAA
                     LRRDFGLRVTADNAAAAAAAGVLVIAVKPQVMPGVARELAGALAGRDTLVISVAAGIR
                     NADLSRWLGGALPVIRAMPNTPALLGCGATVLCAGPGARTSHRETAEAILRAVGSVSW
                     VEDESQLDAVTALSGSGPAYFFLLAEAMADAAAELGLPTELARLLAVETALGAARMAI
                     ESDADIAELRRRVTSPGGTTEAAIDALEAGGFRALVAGAIQRAAARSRELAERFGKE"
     gene            9052..9600
                     /locus_tag="PLS34_05125"
     CDS             9052..9600
                     /locus_tag="PLS34_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRP86887.1"
                     /translation="MNAALFFVIKTLLDLYLLVYLLRFLLQWARADFHNPLSQFILRI
                     TDPVVRPLRRVVPGWKGLDLSPLVALLLLQLLVTAMLTYLATGALASAGMLLYYAVLR
                     IIIGAIRLYFFAILAHVIISWVSPGHWNPLISILHSLTRPLLQPVRRVVPPIGGLDLS
                     PLFVLIGLQALLILLRMPGYLA"
     gene            9603..9908
                     /locus_tag="PLS34_05130"
     CDS             9603..9908
                     /locus_tag="PLS34_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HRP86888.1"
                     /translation="MACRWEDPATLVCRLRVQPRAARDGFAGLMGDRLRLRIKSPPVD
                     GKANDALRRFLAEAFGVPLGQVELIAGAGSRDKLVRVVSPSKIPDEIAAVAAPNRAT"
     gene            9958..10458
                     /locus_tag="PLS34_05135"
     CDS             9958..10458
                     /locus_tag="PLS34_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HRP86889.1"
                     /translation="MTLTKPALAATVASLAAGLVACGQSTEPGARPVPKAQEVTRASF
                     ETFGDYIVHFNAQSTTMLPADVARAFGIRRSGNRAMLNVAVLRRGEAGGDSPVDAAVA
                     VSAVNLLGQEKDVSVRELREGEAIYYIGEFTIANEEIVTFRISVRPAGADAPHEFSFR
                     QQFYTD"
BASE COUNT         1571 a         3569 c         3794 g         1678 t
ORIGIN      
        1 ggcccaggcg acgaagaaga agccgaggta gaccagccag aaatacgccg tccagccctg
       61 gccggactcc ggcggtacga gccagcggtg caggccctgc aggcgcgctc ccaggctcaa
      121 tgcaggcgcg ccgcgcggat ggtgaactcg aaatcgccgg gcgcgccgct gacgaacacg
      181 ccgatggctc gcaggcgggc caggtcgacc ccgtcgagct ccgcgagctc gaggcgcagg
      241 gcgccgtcgc cttccagatc tcgccaggtc ggctgggccc ccggctcgcc gctgaagaac
      301 atgacgcggt agccgtcggc gccttcgagg tcgatttcga gcgtgcgccg gtcggacagg
      361 tcggcggcct gcatgggcac ctcgctggtg ttccacatgg cgccagcata cggaaaagcg
      421 aagccggcgc gcagcacgcc cgacacgacg agtgcgccgg gggcggccgc cgtgtcgatg
      481 gccgcggtcg agcggccctg ctggtactgg tcgctggccg ccaaccaccg ggcatgctgc
      541 cccaccaggt cgagcggtgc gccggcctct ccggcaggcc cctgcgcggc ggccggcgcg
      601 cgcgtcacgg cgcgctcgag cgacacggca tggccgttct tccacacccc ggcgatgcgg
      661 gtggtggcgt cgatgtccgc gagcggattg ccatcgacca gcagcaggtc ggcgcgacac
      721 ccggtgcgca ggcagccccg ccctgccagc gcgaaagcgc gcgccgggac ggaggtcgcg
      781 gcgcgcaggg cgtccaccgg cgcgaggccg gccgcgacca gcagcgccag ctcgtggtgc
      841 aggctggcgc cctgcgcagt gcccgggttg ggcgcgtcgc tgccggccag caggggcacg
      901 ccggcctctt gcagggcccg cacgccggcc atgatcatcg gccagcgggc gcggcgcgcc
      961 tcgccgccgg ggaaggtctg cccgagcgac tgtgcctgcg ccggcgagag gcgcgcgccc
     1021 agcgcggggt gctcgagtag cgcctcggcg gcggagtggc cgtggctcga agccagcaca
     1081 gtggccgtcg gcacaaccca ggcgccggcc gcacgcgccg cctcgatgaa cggcgcgtcc
     1141 accggcctgt cgacgaacag gtgcatcagc ccgtccgcgc ctgcctcgat ggccagtcgg
     1201 gcggcggcgt gcgtcgacac gtgggccacc gccagcacgc cccgcgcatg cgccgcggac
     1261 accagcgccc gcaccgtgtc cggcgcaagc gtgggcaact cgccgcccca gcccgagccg
     1321 tcctcgatga cgatcttgat gtagtcggag ccctcggcga gtctcgccgc cacccaggcg
     1381 tccgcgtccg ctgtggtcgg cgtcggcacg gggaagccgt actgggtgcc gtgcccgccg
     1441 ggcgcggtgg ccaggtagcc ggctgagaac aagtcggcgc gtggcgtcgg cgccgtcgac
     1501 tcgcgctctg agcgtatctg ggcggcatcc agcggcgggc ggaacatgtc gagcagcgtc
     1561 gtcacgccga agcgcagcgc gtcttcgcgc gccgggccga agacgtgcac gtgggcgtcg
     1621 atcagcccgg gcagcgcggt cttgccgcgc ccgtcgatca cctcagcgcc ggccggcacc
     1681 gccaggcccg cgcctgcctc ccgcaccagg ccgtccgcca gcagcaaggt ggcgtgctca
     1741 gtcacttgct cgccgtcgaa cagtcgcaca tcggtgatgg ccgtcacggt tgcgcccgcc
     1801 gcggcatccc cgctggcggt cggcgccggt ccgggcaggg ccagcagggt cagcaacagc
     1861 acggccagca gcgccgcgat gctcagggtg gttctcatca tcgactcctc agcgtcgcga
     1921 gccgtccagc cgccgccagc cgatcacggt ggccgcgccg cacagcaggg tgaacacgcc
     1981 gagattggcc agcgccgcac cccagtccgc ggaccgggcg ccggtgacgt cgagcgccag
     2041 ggcggcgagg tggtagggcg gcagccagcc ggccagtgct tgcaggccgg gcgggaactg
     2101 cgtggcggga atccacaggc cggagagaat ggccagcggc aggtagatga tgttgaccac
     2161 cgccggcgcc gcacggggat caagccacag cccgagcgcc aggcccagca ggcagaacgg
     2221 cagggtgccg gcgaccagca cgcccgccag ccccagccac tgcgcggtgt gcaggcgaac
     2281 gccgccgaac ccggccgcca gcgcgctcat catgacgacg ataaccagcc cgaacagcac
     2341 ggcgccgaag gccttgccga tgatcacggc atataccggc gcgggcgagg cgcgcagcag
     2401 cagcagccac ccctgcgcgc gctccagcgc aatgcccacg ccgaaggaga acaacgcggc
     2461 gcccatgaca ccgaacgtgg cgaaggtgca cagcaaggac agcgactggc ccggcagcat
     2521 caccagcccg aagaaggcgt agaacatgac gggaaacagg agcgtcggca ggctgtaggc
     2581 cggcgtgcgc accaggttga gccactgcgc ctcgacgagc cgcacggcgg gatttgcgag
     2641 catcacgcca cctcccgttc ggtcaatgcg aagaacgtgc tttccaggtc ctcgccggcc
     2701 tccgccagcg ggccgtcatg caggatccgg ccgcggtcca tgaccaccac ccggtcggcg
     2761 aggcgccgcg cttcgtccat gaaatgggtg cagagcagga tcgacgcacc ggcggcgcga
     2821 cgggcctcga tcgcgcgcca cgcctggcgc cgcgccgccg ggtccagccc ggtggtcggc
     2881 tcgtcgagga tcagccagtc ggggcggccg accgcggcca gcgcgaacag cacgcgctgc
     2941 cgctggccgc cggacaggcg ctcgaagcgg cgctgctcca gcccctgcag gccagcctcc
     3001 tcgagcagcg cgtcaaagtc gccgggggcg ccgtagaagc tggcgaacag gcgcagcagt
     3061 tccagcacgg tcaggttgcc ctgcacgccg ctggactgca gcatgacgcc gatccgccgg
     3121 cggatcgccg ggtcggcggg atccagaccg tcgaacagcc ggatgacgcc cgactgcacc
     3181 ggcagcaggc cgagcagcag gtggatgagc gtggtcttgc cggcgccgtt ggggccgagc
     3241 agtgccgtga cctgcccttg cggcagggcc agatcgacgc cgtccagcgc gaccgtccgg
     3301 ccgtaggcat ggcgcacatt gctgcaggtc gcgatgatcg gatgggccat ggctttgctc
     3361 ccgtgactgt ggagcacatc ctcggcggtc gcgggcgggc tgcccagtga cgggcctcag
     3421 gaatcgccgg tgacaaatgt catgcgccgg ccggccgttg actcgcccgc agcgcggtgc
     3481 cagattgccg cgtacagcca cgcgggggag tcatcgccat gaaagaccag gtcatccgcg
     3541 acgcgccggg caagacgccg gtgcatctct ggatcgtcgg tgtgctggcc ttgttgtgga
     3601 atgcggtcgg cgccttcgac tacgcggcga cgcagtttcg cttcgaggcc tacatgggcc
     3661 agttcacgcc ggagcagctg gactatttct acggcatccc ggcctgggcg gtggccgcct
     3721 gggccgtggc ggtgtggttc gcggtgttcg gctcgctggc gctgctgctg cggcgccggg
     3781 ccgcctacac gctgttcctg gtgtcgctgg cggccatggt gaccagcacg gtgcacagct
     3841 tcgtgctgtc cgagggcgcc gcgatcatgg gcagcgaggg cgtggtgttc tccggcctga
     3901 tcgtggtgat cggcgtgctg ctgccgtggt acagcaagat catggccgat cgcggcgtgc
     3961 tgcgctgaga aaactggggt cagacaccca tttcgcgtgc gaaatgggtg tctgaccccg
     4021 gttttttcct cagccgaagt tgatgcgcgc ctcgaggtcc accgaggagt cggcgtaggc
     4081 cagcgcccgc tcgaggtcga tgcgaccttc cttgaagagc ttgtagagcg aggagtcgaa
     4141 gctctgcatg tcgccctggc tggtctcgcg gatcgcctgc ttgatgccgc cgatgtcgcc
     4201 cttcgagatc agctcggcga tgtgcggcgt gttcagcagt acctcgaccg cggccacccg
     4261 cttgccgtcg gtgccgatga tcaggcgctg cgcgatgatc gccgacaggt aatgcgacat
     4321 gtccatcagc acctggcggt gctggctctg cgggaacatg ttgatgatgc ggtcgagcgt
     4381 ctcggcggcg ttgttggcgt gcagcgtggc gatcgccagg tggccggtgc cggccagcgt
     4441 gatcgcggcc tccatggtct cctggtcgcg gatctcaccg atcagcacca cgtccggcgc
     4501 cgcgcgcatg gcgctcttca gcgcgcgcgc gtaggaatgc gtgtcccagc ccagctcgcg
     4561 ctggttgatg atggacttct tgttcgggtg gaggaactcg atcgggtcct cgatggtgat
     4621 gatgtggtca ctgagcagct cgttgcggtg gttcagcatc gccgcgagcg tggtggactt
     4681 gccggagccg gtcgcaccga ccatcaggat caggccgcgc ttcctgaggg ccaggtcctt
     4741 gagcaccttc ggcagcccga ggtcgtcgag cttcggcatc tccagggtga tgtagcgcag
     4801 cacgatggcg atgttgccgc gctggcggaa gacgttggcg cggaaccggc ccaggccggt
     4861 ttccgagatc gcgaaatcca gctcgtgctc gcgctcgagc acgcgcttct gcgcctcggt
     4921 catgatcgac atggcgatct ggttgaccac ctcgaccgac atgacctcct tgccgaccgg
     4981 cacgatctgg ccctcgatct tgatcttgat cggcgccatc ggcgtgaaaa acaggtccga
     5041 ggccgacttg tcggccatca gcttcaggta gggcctgagc ttgaacgcgg acggcggcgg
     5101 cggcgccgtc agcggatcag gcgtcgcggc cataggtttg cgacttctgt tcatctgcga
     5161 gcctcaagct gtcggaaccg tcgtcggcgg ccttgaaatc gcgcttgctc tcgagcttga
     5221 tgcgcaggcg caactcgttg cgggagtcgg cattgcgcag cgcgtcctcg taggagatct
     5281 tgccgtcctc gaacagctcg aacagcgcct ggtcgaaggt cttcatgccg agccggttgg
     5341 agcgcgccat cacttccttg agctgatgca cctcgccctt gaagatgagg tcggagatca
     5401 gcggcgaggc gagcatgact tccatggccg cgatgcggcc gacgccgccg gcgcgcggca
     5461 gcaggcgctg cgcgatgatg cccttgatgt tcagggacat gtccatcagc agctggtcgc
     5521 gcttgtcctc cgggaagaag ttgatgacgc ggtcgagcgc ctggttggcg ctgttggcgt
     5581 gcagcgtggc caggcacagg tggccggtct cggcgaactg gatgccgtac tccatggtgt
     5641 cgcgatcgcg gatctcgccg atcaggatga cgtccggcgc ctggcgcagg gtgttcttca
     5701 gggcgatgcc ccaggactcg gtgtccatgc cgatctcgcg ctgcgtgacc acgcagctct
     5761 tgtgcgtatg cacgtactcg accgggtcct cgatggtgat gatgtggccc ttggagttct
     5821 cgttgcggta gccgagcatc gccgccagcg tggtggactt gccgctgccg gtgccgccga
     5881 cgatcagcac caggccgcgc ttggtcatcg ccacgtcctt caggaccggc ggcaattcca
     5941 gctcctcgaa ggtcgggatg gtggtgttga tggtgcgcat cacggcgccg ctgtggccct
     6001 gctggacgaa ggcgctgacg cggaaacggc cgatgcgggg cggcgcgatc gcgaagttgc
     6061 actccttggt cgcgtcgtat tccttggtct ggcggtcgtt catgatcgag cggaccagcg
     6121 tggcgctctg ctgcggcgtc agcggctggt cggtgaccgg cttgacccgc ccgtccacct
     6181 tgatcgccgg cgggaagccg gcggtgagga acaggtcgga gcccttgacc tcgaccatct
     6241 tgcgcaacaa ctcgtggatg aagcggattg cctggtcgcg ttccattaga aggcttcctt
     6301 gttctgggcc ttgtagcgcg cttcctcgcg gctgacgagg cccttgcgga gcaggtcctg
     6361 gaggcactgg tcgagggtct gcatgccgat cgcctggccg gtctggatcg ccgagtacat
     6421 ctgcgcgatc ttgccctcgc ggatcaggtt gcggaccgca gccgtaccga tcatgatttc
     6481 gtgcgccgcg acgcggccgc cgccgatctt cttcaggagg gtctggctga tgacggcccg
     6541 cagcgactcg gacagcatcg agcgcaccat ctccttttcg ccggccggga acacgtcgac
     6601 cacgcggtcg atggtcttgg cggcggagct ggtgtgcagg gtgccgaaca ccaggtggcc
     6661 ggtctcggcg gcggtcagcg ccaggcggat ggtctcgagg tcgcgcatct cgccgaccag
     6721 gatgacgtcc gggtcctcgc gcagcgccga gcgcagcgcc tcggcgaagc cgagcgtgtc
     6781 gcggtgcacc tcgcgctggt tgatgagcga tttcttgctc tcgtgcacga actcgatcgg
     6841 gtcctcgatg gtgaggatgt gatcggggcg gttgttgttg acgtggtcga tcatcgccgc
     6901 gagcgtggtg gacttgcccg agccggtggg cccggtgacc agcacgatgc cgcgcgggta
     6961 catcgagatg tccttgaaga tcttcggcgc gttcaggtcg tccagcgtca tgatcttgga
     7021 cggaatggtg cggaacacgg cgccggcgcc gcggtgctgg ttgaaggcgt tgacgcggaa
     7081 gcgggccagt tccgggatct cgaacgagaa gtcggtctcg aagaactctt cgtagtcctt
     7141 gcgctgcttg tcgttcatga tgtcgtagac catgctgtgc acttccttgt gcgtcagcgc
     7201 gggcatgttg atgcgcttca cgtcgccgtc gatgcggatg atcggcggca cgccggaaga
     7261 aaggtgcagg tcggaggcac cgttcttgac ggagaaacgg agcagctgtt cgatgtctac
     7321 ggccatcgtc tatcccttga ttctgctgcc ccaacggcct ttgcacgccg tgcggcgacg
     7381 gttcggggag tatacataac atggtgtcgg agaatatgac gagcgtctca gaaagcacgg
     7441 tggcggaacg gctcgccgcc gtccgggcgc gggtggctgc cgcggcgcgc gccgcggggc
     7501 gcgatccggc cgggatctgc atcctcggcg taagcaagcg ccagccgctg gcggcggtgc
     7561 gcgccgcgct cgctgccggg ctggaggatt tcggcgagaa cttcgtccag gaggcgctcg
     7621 gcaagatgga cgcgctcggc cccggccggg cgcgctggca tttcatcggc gcgctgcagt
     7681 ccaacaagac cgccgccgtc gcggagcgct tcgactgggt gcacagcgtg gatcgcgaca
     7741 agctcgccga gcgcctgtcg cggcagcggc cggccgggct gccgccgttg aacgtctgtg
     7801 tgcaggtgca catcggcgac gaggcctcca agtccggcgt cccgccggaa caggccgagg
     7861 cgctggcccg ccgggtggcc ggtttgcccg ggctgcggct gcgcggcctg atggcggtgc
     7921 cgccgccgga agccgacccc gcccgccagc gcgcctggtg ccgccggctc gccgagctgt
     7981 tcgccgcgct caaggcgcgc gggcttgcgc tggacacgct gtccatgggc atgtcggacg
     8041 acctggaggc ggccgtcgcc gaaggcgcca ccgtggtgcg catcggcacc gccatcttcg
     8101 gcccccgaat tggggacatt ccccaaatcc agcaatgaaa ttggggaatg tccccaattc
     8161 atgccttatc cttggcgcat gagacagacc tcgatcgcct tcatcggcgg cggcaacatg
     8221 gcgcgcagcc tggtcggcgg actcattgcc gacggcgtcg gcgcggcgca gctgcaggtc
     8281 gcggagccgg accccgagcg gcgcgccgcg ctgcgacgcg acttcgggct gcgggtgacg
     8341 gcggacaacg cggcggccgc ggcggctgcc ggcgtgctgg tgatcgccgt caagccgcag
     8401 gtcatgcccg gcgtggcgcg cgagctggcg ggcgcgctgg cggggcgcga cacgctggtg
     8461 atctcggtgg cggccggcat ccgcaacgcc gacctgtcgc gctggctggg cggcgccctg
     8521 ccggtgatcc gcgccatgcc gaacacgccc gccctgctcg gttgcggcgc gaccgtgctg
     8581 tgcgccgggc cgggcgccag gaccagccac cgcgaaaccg ccgaggccat cctgcgtgct
     8641 gtcggctcgg tcagctgggt cgaggacgaa agccagctgg acgccgtcac cgcgctgtcc
     8701 gggagcggcc ccgcctattt cttcctgctg gccgaggcca tggcggatgc tgccgcggag
     8761 ctgggcctgc cgacggaact ggcccgcctg ctggcggtcg agaccgcgct gggcgccgcc
     8821 cggatggcca tcgagagcga cgccgacatc gccgagctgc gccgccgggt gacttcgccc
     8881 ggcggcacca ccgaggccgc catcgatgcc ctcgaggccg gcggcttccg ggcgctggtg
     8941 gccggcgcga tccagcgcgc ggcagcgcgc tcgcgtgagc tggccgagcg tttcggcaag
     9001 gagtgaacgg cggccgcgcc gcccgcacgg agactggaga ctgcccccga gatgaacgcc
     9061 gcgctgtttt tcgtcatcaa gacgctgctg gacctctacc tgctggtcta cctgctcagg
     9121 ttcctgctgc agtgggcgcg cgcggatttt cacaatccgc tctcccagtt catcctgcgc
     9181 atcaccgacc cggtggtgcg cccgctgcgc cgggtggtgc ccggctggaa gggcctcgac
     9241 ctgtcgccgc tggtcgccct gttgctgctg cagctgctgg tcacggcgat gctgacgtac
     9301 ctcgcgaccg gcgcgctggc ctctgccggg atgctgctct actacgccgt gctgcgcatc
     9361 atcatcggcg ccatccgctt gtatttcttc gccatcctgg cgcacgtcat catcagctgg
     9421 gtcagcccgg gccactggaa cccgctgatc agcatcctgc acagcctgac ccggccgctg
     9481 ctccagccgg tacgccgggt ggtgccgccg atcggcgggc tcgacctctc accgctgttc
     9541 gtgctcatcg gcctgcaggc gctgctcatc ctgttgcgca tgcccggcta cctggcgtga
     9601 cgatggcctg tcgctgggag gacccggcga ccctggtgtg ccgcctgcgg gtgcaaccgc
     9661 gcgcagcgcg cgacggcttc gccggcttga tgggcgatag actcaggctg cgcatcaagt
     9721 cgcccccggt cgacggcaag gcgaacgacg cgctgcgccg tttcctggcc gaggccttcg
     9781 gcgtaccgct gggtcaagtc gaactgatcg ccggcgccgg cagcagggac aaactcgtca
     9841 gagtcgtctc acccagtaaa ataccggacg aaatcgcggc ggtggccgcg ccgaatcgcg
     9901 cgacatgagc tatagttagc gcgttcgccg ccaagccgcg aacctggagc gccacagatg
     9961 accctcacaa agcccgcact tgccgccacc gtggcatcgc ttgccgccgg cctcgtcgcc
    10021 tgcggccagt cgaccgagcc gggtgcgcgg ccggtgccga aggcgcagga ggtcacgcgc
    10081 gcgagtttcg agactttcgg cgactacata gtgcacttca acgcgcagtc gacgaccatg
    10141 ttgcccgcag acgtggcgcg cgccttcggc atccggcgca gcggcaaccg cgcgatgctc
    10201 aacgtggcgg tgttgcgcag gggtgaagcc ggcggcgatt cgccggtgga cgcggcggtg
    10261 gcggtgagtg ccgtcaacct gctcggccag gagaaggacg tcagcgtgcg ggaattgcgc
    10321 gagggcgagg cgatttatta catcggcgag ttcacgatcg ccaacgagga gatcgtgacc
    10381 ttcaggatca gcgtccgccc cgcgggcgcg gacgcgccgc acgagttcag tttcaggcaa
    10441 cagttctaca cggactgagg caaaccgggt gggccggacc ggacaccggg aagaacaagg
    10501 gccgctgagg accggccgtt cgaccacgga cccgagaaag ctcgtccttc atcatcaaga
    10561 cgaaaaaggc aggagaacca gatggcagtt aagaaggcag ccgccgccgt ag
//
LOCUS       DAOMPG010000066        10558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2699_length_10558_cov_4.134057, whole genome shotgun sequence.
ACCESSION   DAOMPG010000066 DAOMPG010000000
VERSION     DAOMPG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_10558_cov_4.134057"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..685
                     /locus_tag="PLS34_05140"
     CDS             155..685
                     /locus_tag="PLS34_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HRP86890.1"
                     /translation="MKQLVVISAVGGDRSGVVHDLTRAVLDCGGNILDSRMIALGSEF
                     AMLLLVSGSWHTLARLESELKKVEEQSGLSLSMRRTEEREPRKDMLPYAIDVVCLDQP
                     GIVFNLASFFASRDIDIAEMATRSYSAAHTGAPMFSVQITVNIPGNLQISALRDEFMD
                     FCDHLNLDAIMEPVKG"
     gene            707..1174
                     /locus_tag="PLS34_05145"
     CDS             707..1174
                     /locus_tag="PLS34_05145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRP86891.1"
                     /translation="MSTAVVGKKVPAFKLPATGGKQLGLADFKGRKLVLYFYPKDDTP
                     GCTTEGQGFRDHYRAFQKAGAEIVGVSRDSVASHDKFKAKYEFPFELISDPDEVLCSL
                     FNVIREKNMYGRKVMGVERSTFLIDDKGVLRGEWRKVKVPGHVEAVLEAVKAL"
     gene            1283..2638
                     /locus_tag="PLS34_05150"
     CDS             1283..2638
                     /locus_tag="PLS34_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRP86892.1"
                     /translation="MHDPAALFRFDEHDIHLPMVVLEELDAGKKGMSESARNVRQVSR
                     FIDQMIAGADKSHIDHGIEIPGEHRTGPASQVRPGRLFFQTRPNQPLLPDTLPGSRAD
                     NSILENCLMLQEAHPDAQVTLVSKDINLRIKATILGVYAEDYYSDRTLEDLDLLYSGA
                     EALPADFWDTHSKAMDSWKEEGRTFYRVTGERVKHWHVNQFLYLEDDGGFEALVRELD
                     GDSAVIELAANYRGRNSVWGITARNREQNFALNLLMSDSVDFITILGPAGTGKTLLTL
                     AAALAQTLDKARFREIIMTRVTVPLGEDIGFLPGTEEEKMEPWMGALMDNLEVLTHSE
                     EGGDWGRAATQDLLRSRIKIRSLNFMRGRTFLNRFIIIDEAQNLTPKQMKALITRAGP
                     GTKMVCLGNISQIDTPYLTETTSGLTYVVNRFRGWPHSGHATLMRGERSRLADYASDS
                     L"
     gene            2713..4173
                     /locus_tag="PLS34_05155"
     CDS             2713..4173
                     /locus_tag="PLS34_05155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRP86893.1"
                     /translation="MLNRLLIALTACLLTAAAGADPQRDLPDIGSVADTVFTRTQAEA
                     IGRSVLRQLREQGNVLEDPEVAEYITDIGQRIASQAHDGEHRFEFFVVNDDAINAFAL
                     PGGYVGINSGLVTATRSESELAGVLAHEIAHVTQKHIARRIAGTGRTSILATAAVLAA
                     ILLGAGGDAIPAAIMSAQGLAIQEQINYTRANEYEADRIGLGFLARAEFDPMGMPSFF
                     EVLSREAALPGSRVPEFLQTHPLSSNRIAETRDRAERTEARAVRESPMYDLMRARIHV
                     LNARSADDALARFVVQMGDDPEASTPAARYGYGLALLRAGRHAEAVPVLQRLLDEDEA
                     TVVFHSALGEALILAGRTDEGLAVFEHAVRLFPRNRPLVVRYAEALLRTRHFQQAHAV
                     LLDLFNVVRPTPPQVRLIASAADAAGQAAEALFYMSEYHLLTGQLPMALDKLRLALLQ
                     PDLQPWQRARFEARIAELEPYLKQYRASRVRTEQKR"
     gene            4207..4986
                     /locus_tag="PLS34_05160"
     CDS             4207..4986
                     /locus_tag="PLS34_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRP86894.1"
                     /translation="MTISIRRGARFAVAVLLLAATAGCASTPRDAGERDPYDPFEPLN
                     RQVWAFNQAADRAVLRPVARGYDRVVPGSVKWSVANFFDNLGTPIWVLNHLLQGNLPD
                     AGRQSGRFVINSTLGLLGLWDPAGDNGLPRKPARFDQTLGKWGVPPGPFVMMPLLGPS
                     SARGVVGMYARYETDIVWNWLDDNRSLRDKLVVVDIVDTRRRLLPLDGMLERAADSYI
                     LVRDSYRQRAEFAIRGAGSDDDVGLDFEDEDWDDEDWDEGD"
     gene            complement(4990..5337)
                     /locus_tag="PLS34_05165"
     CDS             complement(4990..5337)
                     /locus_tag="PLS34_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP86895.1"
                     /translation="MTGAPSAQPEPGSVVFESLGGGRFLVRGELGFATVRDALEQSKS
                     LFAEHSIIELDLAGVTRADSAGLALLLEWVNWARNSVREVTYRNIPAQVRSIAEISEV
                     EDMLHRAERWTGA"
     gene            complement(5340..5978)
                     /locus_tag="PLS34_05170"
     CDS             complement(5340..5978)
                     /locus_tag="PLS34_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP86896.1"
                     /translation="MNMKTMRTRLAVATLVIGAMTASAAQVAAATPERSPEQVIRDAS
                     EMTMQAIEGRRDELKANRDELFRIIDQVLLPRWDRQYTGQLVMGRAWRDATPEQREAF
                     ISGLYRKLLDSYGEGVLQYNADQLKILGTRGNLEEGPVMVDSEVRLGDGTPVPISYRL
                     RIHEGSWKVYDVVVEGISYVQNYRNQYQSEFRAKGIKGVLADLGAAAGNDGS"
     gene            complement(5982..6449)
                     /gene="mlaD"
                     /locus_tag="PLS34_05175"
     CDS             complement(5982..6449)
                     /gene="mlaD"
                     /locus_tag="PLS34_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRP86897.1"
                     /translation="MQQTRAVELGTGLFVFMGILALFFLTTRVTSFDAYSGDGGYELT
                     ARFDQIGTLKTRSPVSISGVQIGRVTAIDFDVERLEAVVTMRIDSRYDRIPNDSDASI
                     LTSGILGGQYIGLQPGGADEYFRDGDEISFTQSAVVLEQLISKYLFGQAGGGE"
     gene            complement(6454..7221)
                     /gene="mlaE"
                     /locus_tag="PLS34_05180"
     CDS             complement(6454..7221)
                     /gene="mlaE"
                     /locus_tag="PLS34_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HRP86898.1"
                     /translation="MEIGYSLRGFFGTFGAGQLFLLRILANMPYALARPRLIIKQVYV
                     TGVQSLVVIMLAGLFVGMVLALQGYLTLSRFGAEDTIGMVAALGLVRELGPVVTALLF
                     AGRAGTALSSEIGLMRATDQLSGMEMMAVDPIKRVIVPRFLGGVIAMPILALIFSAMG
                     IFGAWLEGVIVLGVDEGAFWAQMQAQVDLREDVMSGVIKSCFFGVAASLLAVFEGYNC
                     VPTAEGVGRATTRAVVHTSLSVLVLNFMLTAWLLGGN"
     gene            complement(7273..8025)
                     /locus_tag="PLS34_05185"
     CDS             complement(7273..8025)
                     /locus_tag="PLS34_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP86899.1"
                     /translation="MIFDGLDIDIRRGSVTAIMGPSGTGKTTLLRLVTGQELPDRGSI
                     RFDGAEVTRMSRRELFRMRKRVGMLFQNGALLTDLNVYDNVAFPLREHTRLPERLIRH
                     IVLTKLHAVGLRGAWQLMPSQLSGGMARRVALARAIVMDPEIIYYDEPFVGLDPISMG
                     VVVRLIRRMNDALGLTSVVVTHDVDEVSSIADCSFLLSEGKVAAFGTPEDLRDHHSET
                     VTQFMTGMADGPVPFHYPAPDYGEQLLARDGK"
     gene            complement(8134..9156)
                     /locus_tag="PLS34_05190"
     CDS             complement(8134..9156)
                     /locus_tag="PLS34_05190"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HRP86900.1"
                     /translation="MNLDGLNKVARAMVAPGKGILAADESHPTIKKRFDTIGVENTEQ
                     NRRAYREMLFTAPGIEAAIGGVILFDETLRQQTVAGVPFPELLAGRGIVPGIKVDAGA
                     KPLAGFPGETVTEGLDGLRKRLEEYRKLGAGFAKWRAVIAIGDGIPSAFCLAANAHAL
                     ARYAALCQEADIVPIVEPEVLMDGDHDLARCEAVTGAALLEVFDALARHRVALEGIVL
                     KPNMVVSGQDCPRRAGVGEVAEATIRCLRRYVPSAVPGIAFLSGGQTEEEATAHLNAM
                     NALGPQPWELSFSYGRALQASALQAWRGDSAKLEAGAGAFAHRAHMNHLARSGQYRAE
                     LESRGR"
     gene            9342..9821
                     /locus_tag="PLS34_05195"
     CDS             9342..9821
                     /locus_tag="PLS34_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018917500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HRP86901.1"
                     /translation="MLKMSRLTDYGTGVLAYLAGAGDRPHSATEVAEQTGLPGATVSK
                     ILKLLTRAGLVTSHRGAQGGYVLARAATDITAADVIDALEGPLALTECSTEAGSCELE
                     ALCRVGTAWQRINIAIRHALDEISLAELASLPVDRGPRVNLRNLVSSPGKTARLERG"
     gene            9840..>10558
                     /locus_tag="PLS34_05200"
     CDS             9840..>10558
                     /locus_tag="PLS34_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRP86902.1"
                     /translation="MASGQQDVEQLVARKYRHGFVTDIESDTVPPGLDESVIRHISTK
                     KGEPEFLLQWRLKAFEHWLTMPEPDWAHVKIAPIDYQAISYYSAPKKKTDGPKSLDEV
                     DPKLLETYDKLGIPLHERARLAGVAVDAVFDSVSVATTFKKKLKEAGVIFCPFSEAVR
                     DYPELLEQYLGTVVPTGDNFFAALNSAVFTDGSFVYVPKGVRCPMELSTYFRINAANT
                     GQFERTLIIADEGSHVSYLEG"
BASE COUNT         1835 a         3663 c         3432 g         1628 t
ORIGIN      
        1 aaaaggagcc cgtgatggta tcgggcgccc ccgggggcgg caagcgccgc cccgccacac
       61 gggccccgcc ggacttcgca gccgggactg ttccgagtac actgcccggc atgacccgcg
      121 ccctgaaaat ttccccgtgt ccggaccgca gccgttgaaa caactggtcg tgatctccgc
      181 ggtcggcggc gaccgctccg gcgtcgtgca cgacctgacc cgcgcggtgc tggactgcgg
      241 cggcaacatc ctggacagcc ggatgatcgc gctcggctcc gagttcgcca tgctgctgct
      301 ggtgagtggc agctggcaca cgctggcgcg gctggagtcc gagctgaaga aggtggagga
      361 gcagtccggg ctgtcgctgt cgatgcggcg caccgaggag cgtgagccgc gcaaggacat
      421 gctcccctac gccatcgacg tcgtctgcct cgaccagccg ggcatcgtgt tcaacctggc
      481 gagcttcttc gccagccgcg acatcgatat cgccgaaatg gcgacgcgca gctactccgc
      541 ggcacatacc ggcgcaccga tgttctcggt gcagatcact gtcaatattc cgggcaacct
      601 gcagatctcg gccctgcgcg acgagttcat ggacttctgt gaccacctga acctcgacgc
      661 catcatggag ccggtcaagg gctgaccgaa gtcaaggaga cacaccatgt ccacggccgt
      721 cgtcggcaag aaagtccccg cattcaagct tcccgccacc ggcggcaagc agctcgggct
      781 ggcggatttc aagggccgca agctggtgct gtacttctat cccaaggacg acaccccggg
      841 ctgcaccacc gaggggcagg gttttcgcga ccactaccgc gctttccaga aggccggcgc
      901 agagatcgtc ggcgtgtcgc gcgacagcgt cgcgtcgcac gacaaattca aggccaagta
      961 cgagttcccc ttcgagctca tctcggatcc cgacgaggtg ctctgctccc tgttcaacgt
     1021 gatacgcgag aagaacatgt acgggcgcaa ggtgatgggc gtcgaacgca gcaccttcct
     1081 gatcgacgac aagggcgtgc tgcgcggtga atggcgcaag gtcaaggtgc cgggccacgt
     1141 cgaagccgtg ctcgaggcgg tcaaggcgct ctgatccgcc cacgcatgat ttttccccaa
     1201 tcgccggccg gccacggcag gagtcccatt gagcaccacg gctgaacccc gccgcatctt
     1261 cgtgctggac accaacgtcc tgatgcatga tcccgccgcc ctgttccgct tcgacgagca
     1321 cgacatccac ctgccgatgg tggtgctcga ggaactggac gccggcaaga aaggcatgtc
     1381 cgagtcggca cgcaacgtcc gccaggtcag ccgcttcatc gaccagatga tcgccggcgc
     1441 ggacaagagt catatcgacc acggcatcga gattcccggc gagcaccgta ccggccccgc
     1501 cagccaggtc cggccgggcc ggttgttctt ccagacccgc cccaaccagc cgctgctgcc
     1561 ggacaccctg cccggcagcc gggccgacaa ctccattctc gagaactgcc tgatgctcca
     1621 ggaggcgcat ccggatgcgc aggtgacgct ggtgtcgaag gacatcaacc tgcgtatcaa
     1681 ggcgaccatt ctgggcgtgt atgcggagga ctattacagc gaccggaccc tcgaggacct
     1741 cgacctgctg tattcgggcg ccgaggcctt gcccgccgac ttctgggaca cccacagcaa
     1801 ggcgatggac tcctggaagg aggagggccg gaccttttac cgggtcaccg gcgagcgcgt
     1861 gaagcactgg cacgtcaacc agttcctgta cctcgaggac gacggcggct tcgaggcgct
     1921 ggtgcgcgaa ctcgacggcg actcggcggt gatcgagctg gccgccaact accgcggccg
     1981 caacagcgtg tggggcatca cggcacgcaa ccgggagcag aatttcgcgc tcaacctgct
     2041 gatgtcggac agcgtggatt tcatcacgat tctcgggccg gcgggcacgg gcaagaccct
     2101 gctgacgctg gccgccgcgt tggcccaaac gctcgacaag gcacgctttc gcgaaatcat
     2161 catgacgcgc gtgaccgtcc ccctgggcga ggacatcggc ttcctgcccg gcaccgagga
     2221 agaaaagatg gagccctgga tgggcgccct gatggacaac ctcgaggtgc tcacccacag
     2281 cgaggaaggc ggcgactggg gccgcgccgc gacccaggac ctgctgcgca gccggatcaa
     2341 gatccgctcg ctgaatttca tgcgcggacg caccttcctg aatcgcttca tcatcatcga
     2401 cgaggcacag aacctgaccc cgaagcagat gaaggcgctc atcacgcgcg ccgggcccgg
     2461 caccaagatg gtctgcctgg gcaacatctc gcagatcgat accccgtacc tgaccgagac
     2521 gacgtcgggc ctgacttacg tggtcaaccg gttccgcggc tggccgcatt caggtcacgc
     2581 cacactgatg cgcggcgagc gctcccggct cgcggactac gcctctgact ctctctagcg
     2641 accggggaac cgtggcggcg cccgcagtgt cctaacctgg tgccgccacg aatcaccggc
     2701 tccgggaccc gcatgctcaa ccgcctcctc atcgccctga ccgcctgcct gctgacggcc
     2761 gccgctggtg ccgatccgca gcgcgacctg ccggacatcg gcagcgtggc ggacaccgtg
     2821 ttcacgcgca cccaggccga ggcgatcggg cggtccgtgt tgcgccagct gcgcgagcag
     2881 ggcaacgtgc tcgaggaccc ggaggtggcc gagtacatca ccgatatcgg ccagcggatc
     2941 gcctcgcagg cgcacgacgg ggagcaccgc ttcgaattct tcgtggtcaa cgacgacgca
     3001 atcaacgcct ttgcactgcc tggcggctat gtcgggatca acagcggcct ggtgaccgca
     3061 acacgcagcg aaagcgagct ggccggcgtg ctggcgcacg aaatcgcgca cgtgacccag
     3121 aagcacatcg cgcgacgcat tgccggcacg ggccggacca gcatcctggc cacagccgcg
     3181 gtgctcgccg ccatcctgct cggggcgggc ggcgacgcca ttcccgcggc gatcatgagc
     3241 gcccagggcc tggccatcca ggaacagatc aactatacgc gggccaacga gtacgaagcc
     3301 gaccggatcg ggctcggctt cctggcgcgc gccgagttcg atccgatggg catgccgtcc
     3361 ttcttcgagg tgctgtcgcg ggaggccgcg ctgcccggct cccgcgtgcc cgagtttctc
     3421 cagacccatc cgctcagcag caaccgcatc gcggaaacgc gcgatcgcgc cgagcgcacc
     3481 gaggcgcgcg cggtgcgcga aagtcccatg tacgacctga tgcgcgcccg catccatgtg
     3541 cttaacgcgc gctccgccga cgatgcgctg gcacgcttcg tcgtccagat gggtgacgat
     3601 ccggaggcgt ccacgccggc ggcgcgctac ggctacggcc tggccctgct ccgcgccggc
     3661 cgccatgccg aggcggtgcc ggtgctgcag cggctgctgg acgaggacga agcgaccgtc
     3721 gtgttccatt cagccctcgg cgaggcgctc attctcgccg gccgcaccga cgagggcctg
     3781 gcggtattcg agcatgccgt ccgcctgttt ccgcgcaacc ggccgctggt agtgcgctat
     3841 gccgaggccc tgctgcggac caggcacttc cagcaggcgc atgcggtgtt gctggacctc
     3901 ttcaacgtcg tgcgcccgac gcccccgcaa gtgcgcctga tcgccagtgc cgcggacgcg
     3961 gccggccagg ctgcggaggc gctcttctac atgtccgagt atcatctgct taccggacag
     4021 ctaccgatgg cgctggacaa gctgcgcctc gccctgctcc agcccgacct gcagccgtgg
     4081 caaagggcgc gcttcgaggc ccgcattgcg gagctcgagc cgtacctgaa gcagtaccgt
     4141 gccagccggg taaggaccga acagaagaga tagcaggccg aagagagcgg aatcaggcaa
     4201 atggaaatga cgatttcgat acgccgcggg gcacgcttcg ccgtggccgt gctcctgctg
     4261 gccgcgacgg ccggctgcgc ctcgacgccg cgcgacgccg gtgagcgtga tccctacgat
     4321 cccttcgagc ccctcaaccg gcaggtatgg gccttcaacc aggcggctga ccgcgccgtg
     4381 ctgcgcccgg tggcacgcgg ttacgaccgg gtcgtgccgg ggtcggtcaa gtggagcgtg
     4441 gcgaacttct tcgacaacct gggcacaccc atctgggtgc tgaatcacct gctccagggc
     4501 aacctccccg acgccggccg gcagagcggg cgtttcgtga tcaattccac cctcgggctg
     4561 ctcggcctgt gggatccggc cggcgacaac ggcctgccgc gcaagccggc ccgtttcgac
     4621 cagacactcg gcaaatgggg cgtgccgccc ggcccattcg tcatgatgcc cctgctcggc
     4681 ccgagttcgg cgcgcggcgt cgtcggcatg tacgcgcgct acgaaacgga catcgtctgg
     4741 aactggctcg acgacaaccg cagcctgcgg gacaagctcg tggtcgtcga catcgtcgac
     4801 acgcggcgcc gcttgctgcc gctggacggc atgctcgagc gcgccgcgga ttcctacatc
     4861 ctggtcaggg attcctaccg ccagcgcgcc gagttcgcga tccgcggcgc tggcagcgat
     4921 gacgacgtcg ggctggactt cgaggacgag gactgggacg acgaagactg ggacgaaggc
     4981 gactagggac taggcgccgg tccagcgctc cgcgcgatgg agcatgtcct cgacctcgct
     5041 gatttccgcg atcgagcgca cctgtgcggg aatgttccgg taggtgacct cccgaacgct
     5101 gttgcgcgcc cagttgaccc actccagcag cagcgcaaga ccggcgctgt cggcccgcgt
     5161 cacgcccgcc aggtcaagct cgatgatcga gtgctctgca aacagcgact tgctctgttc
     5221 cagcgcatcg cgcaccgtag caaaccccag ctcaccgcgg accaggaagc gaccgccgcc
     5281 cagcgactcg aacaccacgc tgccgggctc gggctgggcg gacggcgcgc ccgtcacctt
     5341 caggacccgt cgtttccggc ggccgcgccg aggtccgcca gcacgccctt gatgcccttg
     5401 gcgcggaact cgctctggta ctggttgcgg tagttctgca cgtaggagat gccctcgacc
     5461 accacgtcgt agaccttcca gctgccttcg tggatccgca gccggtagct gatcggcacc
     5521 ggcgtgccgt cgccgaggcg cacttcggag tcgaccatca ccgggccctc ctccaggttg
     5581 ccgcgcgtgc cgagaatttt cagctggtcg gcgttgtact ggaggacgcc ctcgccgtag
     5641 ctgtcgagca gcttgcgata gagaccggaa atgaatgcct cgcgctgttc gggcgtggcg
     5701 tcacgccagg cacggcccat taccagctgg ccggtgtact ggcggtccca gcgcggtaac
     5761 agcacctggt cgatgatccg gaacagctcg tcgcggttcg ccttgagctc gtcgcgccgc
     5821 ccctcgatcg cctgcatggt catttccgaa gcgtcccgaa tgacctgctc cgggctgcgc
     5881 tcgggcgtcg ccgccgccac ctgtgcggcc gacgccgtca tggcgccgat gacgagggtc
     5941 gccaccgcca gtctcgttcg catcgttttc atgttcatcg tctactctcc cccgccggcc
     6001 tggccgaaca ggtacttgct gatcagttgc tccagcacca cggcggactg ggtgaatgaa
     6061 atctcgtcgc cgtcccggaa gtactcgtcc gccccgcccg gctgcaggcc gatgtactgt
     6121 ccgccgagaa tgcccgacgt caggatactg gcgtccgaat cgttcgggat ccggtcgtag
     6181 cgagagtcga tccgcatcgt caccaccgcc tccagccgct cgacgtcgaa gtcgatcgcc
     6241 gtcacccggc cgatctgtac cccggagatg gacaccggcg accgcgtttt caaggtcccg
     6301 atctggtcga agcgggccgt cagctcgtag cccccgtcac ccgagtaggc gtcgaagctg
     6361 gtaacccgcg tggtcaggaa gaacagcgcc agtatgccca tgaagacaaa caacccggtg
     6421 ccgagttcca cggcgcgtgt ctgttgcatc gctctagtta cctcccaaca gccaggcggt
     6481 gagcatgaag ttcaggacca ggaccgacag cgacgtgtgt accacggcgc gcgtcgtggc
     6541 acgacccacc ccctcggcgg tgggcacgca attgtacccc tcgaacaccg ccagcaggct
     6601 ggcggccacc ccgaaaaagc agctcttgat gacgcccgac atgacgtcct cgcgcaggtc
     6661 cacctgggcc tgcatctgtg cccagaaggc gccctcgtcc accccgagca cgatcacgcc
     6721 ctccagccag gcgccgaaaa tccccatcgc gctgaagatc aacgccagga tcggcatggc
     6781 gatgacgcca cccaggaagc gcggcacgat cacccgcttg atcgggtcga cggccatcat
     6841 ctccatgccg gacagctggt ccgtggcccg cattaggccg atctcggacg acagcgcggt
     6901 ccccgcgcgg ccggcgaaca gcagcgccgt gaccaccggg ccgagctcgc gcaccagccc
     6961 cagcgccgcc accatgccga tggtgtcctc ggcgccgaac cgcgacagcg tcaggtagcc
     7021 ctgcagggcc agcaccatgc cgacgaacag gcccgccagc atgatcacca ccagcgactg
     7081 cacgccggtc acgtacacct gcttgatgat gagccgcggg cgcgccagcg cgtagggcat
     7141 attggcgagg atgcgcagca ggaacagctg gccggcgccg aaagtgccga aaaagccgcg
     7201 cagcgagtag ccgatctcca gccacaggcg gcgcaggccg cgcgcattgt ccctgctcac
     7261 ggccgcacgc ccctatttgc cgtcacgggc aagcagctgc tcgccgtaat cgggggcggg
     7321 atagtgaaac ggcaccgggc cgtcggccat gccagtcatg aactgggtga ccgtctcgga
     7381 atggtggtcg cgcaggtcct ccggggtgcc gaaggcggcg accttgccct ccgacagcag
     7441 gaaactgcag tcggcgatcg acgagacttc gtcgacgtcg tgcgtgacca cgacactggt
     7501 gagccccaac gcatcgttca tgcgccggat cagccggact acgacgccca tggaaatcgg
     7561 gtccagcccg acgaagggct catcgtagta gatgatctcc gggtccatga cgatggcgcg
     7621 cgccagcgcg acgcgccgtg ccatgccgcc ggagagctgc gacggcatca gctgccaggc
     7681 gccacgcagc ccgacggcat gcagcttggt caggacgatg tgcctgatga ggcgctccgg
     7741 cagtctggtg tgctcgcgca acgggaaggc cacgttgtcg taaacattga ggtccgtgag
     7801 cagcgcgccg ttctggaaca gcatgccgac gcgtttgcgc atgcggaaca gctcgcgccg
     7861 gctcatacgg gtaacctcgg cgccgtcgaa gcggatactg ccccggtcgg gaagctcctg
     7921 cccggtgacc agccgcagca gcgtcgtctt gccggtgccg ctcgggccca tgatggcggt
     7981 cacgctgccg cgccggatgt cgatgtccag gccgtcgaag atcatggtct ggccgcgcca
     8041 ataggcgagg tcgcggatga ccaggatgct gtcgccgccc ggacagccgg cgacgggccg
     8101 cgagcgcagc tggccggcgc cggcgtcgac cactcagcgc ccccgggact cgagctcggc
     8161 ccggtactgg ccgctgcggg cgaggtggtt catgtgggcg cggtgcgcaa aggcgccggc
     8221 tcccgcctcg agcttcgcgc tgtcaccgcg ccaggcctgc agggcggatg cctgcagcgc
     8281 ccgcccgtag gagaacgaca gctcccacgg ctgcggcccc agggcattca tggcattgag
     8341 atgagcggtg gcctcttcct ccgtctggcc gcccgacagg aaggcgatgc ccggcacagc
     8401 cgagggcacg tacctgcgca ggcaacgaat cgtcgcctcg gccacttcgc cgaccccggc
     8461 ccgccgcggg cagtcctgcc cggagacgac catgttgggc ttgaggacga tgccctcgag
     8521 cgccacccgg tgacgggcga gcgcatcgaa cacttccagc agggcggcac cggtcacggc
     8581 ttcgcagcgg gcgaggtcgt gatcaccgtc catcagcact tccggctcga cgatcggcac
     8641 gatgtctgcc tcctggcaaa gcgccgcgta gcgcgccagg gcgtgggcat tggccgccag
     8701 gcaaaaggcg ctcggaatac cgtcgccgat ggcgatcacg gcccgccact tggcaaagcc
     8761 ggcacccagc ttgcggtact cctcgaggcg cttgcgcagc ccgtccagtc cctcggtgac
     8821 ggtctccccg gggaagcctg ccagtggctt ggcgccggcg tcgaccttga tgccggggac
     8881 gatcccgcga ccggccagca gctccggaaa cggcacgccg gcgaccgtct gctggcgcag
     8941 ggtctcgtcg aacaggatca cgccgccgat cgccgcctcg atgccaggcg ccgtgaacag
     9001 catctcccgg taggcacgcc ggttctgctc ggtattctcc acgccgatgg tgtcgaaacg
     9061 cttcttgatg gtcggatggc tctcgtctgc cgccaggatc cccttccccg gggccaccat
     9121 cgcgcgggca accttgttca atccatcgag gttcatcgca tcctccaggg tctcgggaac
     9181 gtgcggcgga atggtcggct ccgaccgctt tgccacggcg aaatcctaac acagcggcgg
     9241 acccgggccc ggcgccgacc accccccggg gttggcctcg gggccaatca ggactaaaat
     9301 ggtccccatt atctcgcgag ccgctttggc cggggacgcc gatgctgaaa atgagccgac
     9361 tgaccgacta cggaaccggg gtgctggcgt acctggcggg cgcaggcgat cgcccccaca
     9421 gcgcgaccga ggtggccgaa cagaccgggc tgccgggagc cacggtgagc aagatcctca
     9481 agctgctgac gcgtgccggg ctggtgactt cgcatcgcgg ggcgcagggc gggtacgtgc
     9541 tcgcgcgcgc agccaccgac atcaccgccg ccgacgtcat cgatgcgctg gaggggccgc
     9601 tggccctgac cgagtgctcg acggaggccg gcagctgcga actcgaggcg ttgtgccgcg
     9661 tgggcaccgc gtggcagcgg atcaacatcg ctatccgcca tgcgctggac gaaatcagcc
     9721 tcgccgagct ggccagcctg ccggtggacc gcgggccgcg ggtgaacctg agaaacctgg
     9781 tgtcgtcgcc gggcaagacg gcgcgactgg agcgcggctg aggccgcggg gagcaacaca
     9841 tggcaagcgg acagcaggac gtcgaacaac tcgtcgcgcg caagtaccgg cacggcttcg
     9901 ttacggacat cgaatccgac acggtgccgc ccggcctcga cgaatcggtc atccggcaca
     9961 tctcgacgaa aaaaggcgag ccggagttcc tgttgcagtg gcgactgaaa gctttcgagc
    10021 actggctgac catgccggag ccggactggg cacacgtgaa gatcgcaccg atcgactacc
    10081 aggcgatctc gtactattcc gcgccgaaaa agaagaccga cggcccgaag agcctcgacg
    10141 aagtcgaccc gaagctgctc gagacctacg acaagctggg catcccgctg catgagcgcg
    10201 cccggctggc cggcgtcgcg gtggacgcag tgttcgacag cgtctcggtg gccacgacct
    10261 tcaagaaaaa gctcaaggag gcaggggtga ttttctgccc gttctccgag gcggtgcggg
    10321 attatccgga gctgctcgag cagtatctcg gcacggtggt gccgaccggc gacaatttct
    10381 tcgcggcgct caactcggca gtgttcaccg acggctcctt cgtctacgtg ccgaagggcg
    10441 tgcgctgccc catggagctg tcgacttact tccgcatcaa cgctgcgaac accgggcagt
    10501 tcgagcgcac gctgatcatc gccgacgagg gcagccacgt gagctacctc gagggctg
//
LOCUS       DAOMPG010000067        10376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2762_length_10376_cov_4.575041, whole genome shotgun sequence.
ACCESSION   DAOMPG010000067 DAOMPG010000000
VERSION     DAOMPG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_10376_cov_4.575041"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..480)
                     /gene="fur"
                     /locus_tag="PLS34_05205"
     CDS             complement(49..480)
                     /gene="fur"
                     /locus_tag="PLS34_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HRP86903.1"
                     /translation="MESKELRKAGLKVTLPRLKILEILEANQQRHMSAEDVYKFLLES
                     EAEIGLATVYRVLTQFEAAGLVRRHNFESGSAVFELDDGGHHDHIICQQCGHVEEFVD
                     STIEARQEAAAAAHGFTVADHSLVIYGNCTRKNCPHREKAD"
     gene            559..933
                     /gene="bamE"
                     /locus_tag="PLS34_05210"
     CDS             559..933
                     /gene="bamE"
                     /locus_tag="PLS34_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRP86904.1"
                     /translation="MPRLIQIALLAFVVMLAGCSSSRGFSLLPYRMNIQQGNYLEAKD
                     VDQIEIGMTQSQVRFLIGTPMVTDPFNQARWDYVFFFKVGRTREEISNRLTIWFEDDR
                     VARIDRPDSLDETVPIETGFDG"
     gene            complement(938..1267)
                     /locus_tag="PLS34_05215"
     CDS             complement(938..1267)
                     /locus_tag="PLS34_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HRP86905.1"
                     /translation="MSIDDTIEVEVAYARPERQLILKLEVPAGTTAIEAARLSGIEEQ
                     FPEIDLGNTRLGVFGKLCKADRPLNAGDRVEIYRPLLVDPRAARRELAAAGKSMGKGG
                     RDDAGED"
     gene            complement(1276..2658)
                     /locus_tag="PLS34_05220"
     CDS             complement(1276..2658)
                     /locus_tag="PLS34_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRP86906.1"
                     /translation="MAKRTSLHGLWSSRLAFILAVSGSAVGLGNIWKFPYVAGANGGG
                     AFVLFYLLCVALIGLPIMMAEILIGRRGRRNPVATMRIVGEEEGSRPSWALVGVMGVI
                     AGIIILSFYSVIAGWSLSYIGHAAMNRFSGAGADEVNGLFGGMLSNWKVLAFWHTLFM
                     TLTIFVVARGVERGLERAVRFLMPSLAILMVALLGYAIINGDFRGGVDFLLEADFSKL
                     TGEGMLAALGQAFFSLSIGMGAIMAYGAYLPEGTSIANTSISVVLADTAMALLAGLAI
                     FPIVFAHGLDPAAGPGLIFQTLPLAFGNMAGGHLFGTLFFVLLTFAAWTSSIGLIEPA
                     VAWKVETRGRSRAVAAIVIGVVVWALGFLTILSFNELENFKVLGRTLFENLDHLSSNI
                     MLPLGGLFITIFAGWVMCKNSTAEELDIGTGGMYRSWRFLARWVAPAAVLLVFLHATG
                     LLRYFGVAAS"
     gene            2777..3259
                     /gene="smpB"
                     /locus_tag="PLS34_05225"
     CDS             2777..3259
                     /gene="smpB"
                     /locus_tag="PLS34_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRP86907.1"
                     /translation="MSTAKKKAASGTGGSTIALNKKARHDYFIEESFEGGLVLEGWEV
                     KSLRAGRAQIAEAYVVVRGGEIWLVGANITPLNTASTHVHPEPGRSRKLLLNRREIDR
                     LVGSVERAGYTMVPLALYWKKGRAKLEIGLAKGKKQHDKRATERDRDWQRQKARILKH
                     "
     gene            3324..3734
                     /locus_tag="PLS34_05230"
     CDS             3324..3734
                     /locus_tag="PLS34_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP86908.1"
                     /translation="MVAITRLMSTSPVTVEPDDTLATVKEIFDRLKFHHLPVVEDYRL
                     VGLVSDRDLLKALSPHLGSAAETARDLATLNKRVHQVMGRNPVTLPATATVWDAVRVF
                     NRDGVACIPIVDDRHQPLGILTWRDVMGALDREG"
     gene            complement(3706..4824)
                     /locus_tag="PLS34_05235"
     CDS             complement(3706..4824)
                     /locus_tag="PLS34_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP86909.1"
                     /translation="MAVPDVECAVVGAGVVGLAVATALARCGIETLLLEAEDRPGEAT
                     TSRNSGVIHAGLYYPPGSLKATLCGRGRDMLYAYCAQRGIPHRRLGKLIVATHPGEAA
                     ALRRLFERGLENRVRLEWLDGAAVRALEPALRCEAAIHSPDSGIIDSAELVMALSGDL
                     QEAGGLLLCHARVTRIGRSDGLFEIETADGERLSCRWLVNSAGLGATALAAATSGLAP
                     EYVPRLWHGAGQYYQAHRRVPFSRLIYPLPVPGALGMHLGFDLAGRARFGPDLRFVER
                     MDYGFDDSLRGRFADAIRVWWPELRDEDLSPDFVGIRPKLVGPGEDSPDFLVQDESAH
                     GIAGLVNLFGIDSPGLTASLALGEHVAALVSPRGRGRP"
     gene            complement(4828..5733)
                     /locus_tag="PLS34_05240"
     CDS             complement(4828..5733)
                     /locus_tag="PLS34_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP86910.1"
                     /translation="MTLSNEQRALSLALGAVLLWSTVATAFKLSLQYLSPVQLLAWAS
                     VVSLLTLGALLVATGRLAEVLRGSRRDYSRSLALGLVNPLCYYLVLFEAYDRLPAQEA
                     QPLNYTWAFTLAILAVPLLGHRLTRADVVGGLVAYAGVWVIATRGEVLALEFANPVGV
                     ALALGSTVLWALYWIYSTRDQRDPLVALFCNFALGLPFVLLACALTDGLAVPDWRGLA
                     GAAWVGVFEMGLAFALWLSALRLTQSASRIGNLVFLSPFLSLFFIRAFVGEAILPSTW
                     AGLGLIVAGLAVQQSFRPPRAAAVV"
     gene            5949..6788
                     /locus_tag="PLS34_05245"
     CDS             5949..6788
                     /locus_tag="PLS34_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRP86911.1"
                     /translation="MGAAWGLPYAGTFTLLFWRYLALAVFLGAVLAWRRSLAWPGLAA
                     VGHAALVGVLAHGTWLACVLVSLERGVPAGIVALVVALQPLATGALSGLVTGESTSAR
                     QWAGLALGFVGVAVAVGARIDWSDTGSAFAYLVPLGSVIAITVASLLQRRREVLGRRD
                     LPLGSALFYQCAATALVLFVPAVVVEGLAARWAPEFLATMAWLIFAVSLGAYGLMWRL
                     LEDLDAARVASLFYLGPPVTMFMGWLAFGDRLQATDLAGMLIAMLGVILVYQRREPRR
                     SKP"
     gene            complement(6755..7291)
                     /locus_tag="PLS34_05250"
     CDS             complement(6755..7291)
                     /locus_tag="PLS34_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP86912.1"
                     /translation="MSFEFVTERLRIRPWRAADRPALERMVRDPDMMRYITQGRTWAD
                     EEVDEILERQARHLKNHGVCFGAVELLATGEVIGLVGLQPHDDGEFELGWWIWKEHWG
                     RGYATEAARPFIIHARDVMGLDRLVAVIDPPNTASIKVAEHLGMSFECVKSANETIAK
                     RPDIPIAYYGLDLRGSRR"
     gene            complement(7335..8162)
                     /locus_tag="PLS34_05255"
     CDS             complement(7335..8162)
                     /locus_tag="PLS34_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86913.1"
                     /translation="MPYPRAHWLLLVLIPAIGVAFWPGYFSRLGASPLAHHVHGVTGS
                     LWILLLVLQSWAIHNRRFALHRAAGRSSLLLFPLFMAGGLMALHAMATGFVAQAHPFY
                     TLFGARLGLLDFIAMTAFAWLFYGALRHRGKVQLHARYMLATPLLLLAPIVSRLLTDF
                     VPYFAIKGPEDMGVFFANLHLSNSVAIVAALLLYASSPRHGRPFLVVAGVALLQGAAF
                     QWLAVTPGWQALFSAYAGMPLLLTAAAGLAVGLALVYAGWTGPRSAGRPPVPERAPS"
     gene            8297..8658
                     /gene="ssrA"
                     /locus_tag="PLS34_05260"
     tmRNA           8297..8658
                     /gene="ssrA"
                     /locus_tag="PLS34_05260"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            8973..9170
                     /locus_tag="PLS34_05265"
     CDS             8973..9170
                     /locus_tag="PLS34_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86914.1"
                     /translation="MNQLLQQWPSGTVATLERVAFKMMHLDRSKVEFASGRRTIHAGG
                     RLDKRYDLVVADAGPGEALAS"
     gene            9170..9495
                     /locus_tag="PLS34_05270"
                     /pseudo
     CDS             9170..9495
                     /locus_tag="PLS34_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
     gene            complement(9534..>10376)
                     /locus_tag="PLS34_05275"
     CDS             complement(9534..>10376)
                     /locus_tag="PLS34_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRP86915.1"
                     /translation="GVLSGSLALIADALHNFSDMASLVIAYAARRIARRPADSRMTFG
                     YARIEVVAALINYTTLIIVGVYLVYEGALRLIDPPEVAGWTVVVVGTVALLVDTLTAL
                     LTWSMQKGSMNIRALFLHNLSDALASVGVVAGGTLILLYDWWVVDPIVTLAIAGYILY
                     LSINEIGGPIRTLMLGSPPAIDADILIRELCTIDEVSGIHHVHLWQMQEHEAALDCHV
                     VLTDIGWGRQEAIKSDIKETLKNRFDISHSSLEFEHVDRAHQDVGRFCDSGGTGHDAS
                     GHSN"
BASE COUNT         1844 a         3524 c         3368 g         1640 t
ORIGIN      
        1 aaatgcgcaa gcgggccgcc cgcgagggct gagcggcggc gggagttctc agtcggcctt
       61 ttcgcggtgc gggcagttct tgcgcgtgca gttgccgtag atgaccagcg agtggtcggc
      121 gaccgtgaag ccgtgcgcgg cggccgccgc ttcctggcgc gcctcgatgg tgctgtccac
      181 gaattcttcg acgtggccgc attgctggca gatgatgtgg tcgtggtggc caccgtcgtc
      241 cagctcgaac accgcgctgc cgctctcgaa gttgtggcgg cgtaccagcc ccgccgcctc
      301 gaactgggtc agcacccggt acaccgtggc gagcccgatc tcggcctccg attccagcag
      361 gaatttgtag acgtcctcgg cgctcatgtg gcgctgctgg ttggcctcga gaatctcgag
      421 aatcttcagc cgcggcaggg tgaccttcag gccggccttt ctcagttcct tcgattccat
      481 gcacggctcc cgcagcaagg accaatcact ctggtattat cgcccactgt ccgccccagc
      541 accaactccc gggaccccat gccacgcctg atccagattg ctctccttgc cttcgtcgta
      601 atgctggccg gctgcagctc gtcgcgaggg ttctcgctgc tgccctaccg catgaacatc
      661 cagcagggta attacctcga ggccaaggac gtcgaccaga tcgagatcgg catgacccag
      721 agccaggtgc gctttctcat cggcacgccg atggtcaccg accccttcaa ccaggcgcgc
      781 tgggattacg tgttcttctt caaggtggga cgcacgcgcg aggaaatcag caaccggctg
      841 accatctggt tcgaggacga tcgcgtggcg cgcatcgacc ggccggattc gctcgacgag
      901 accgtgccca tcgaaacggg cttcgacggt tgagccgcta gtcctcgccc gcgtcgtcgc
      961 gcccgccctt gcccatgctc ttgccggcgg ccgccagctc gcggcgtgcc gcgcgcgggt
     1021 ccaccagcag cggccggtag atttccaccc ggtcgcccgc gttcagcggc cggtcagcct
     1081 tgcaaagctt gccgaacacc ccgagccggg tgttgccgag atcgatctcc gggaactgct
     1141 cctcgatgcc ggacagccgc gccgcctcga tggcggtggt gcccgcgggc acttccagct
     1201 tgaggatcag ctggcgctcg gggcgtgcgt acgccacctc gacctcgatg gtgtcgtcga
     1261 tgctcaccgg ccggctcagg aggccgccac gccgaaatag cgcaacaggc cggtggcgtg
     1321 caggaacacc agcagcacgg cggccggcgc cacccaacgc gccaggaaac gccagctgcg
     1381 gtacatgccg ccggtgccga tgtcgagctc ctcggcggtg gagttcttgc acatcaccca
     1441 gccggcgaag atggtgatga acagcccgcc gagcggcagc atgatgttgc tggagagatg
     1501 gtccaggttc tcgaacagcg tccggcccag caccttgaag ttctccagtt cgttgaacga
     1561 caggatggtc aaaaagccga gcgcccagac caccacgccg atgacgatcg cagccacggc
     1621 gcggctgcgg ccgcgggtct cgaccttcca ggcgactgcg ggctcgatca ggccgatgga
     1681 cgaggtccag gccgcgaagg tcaacagcac gaagaacagg gtgccgaaca ggtggccgcc
     1741 ggccatgttg ccgaaggcca gcggcagcgt ctggaagatc aggccgggcc cggccgccgg
     1801 gtccagcccg tgggcgaaca cgatggggaa gatcgccagg ccggccagca gcgccattgc
     1861 ggtgtcggcc aacaccacgg agatcgaggt gttggcgatc gaggtgccct ccggcaggta
     1921 ggcgccgtag gccatgatcg cgcccatgcc gatgctcagg ctgaaaaagg cctggccgag
     1981 cgcggccagc atgccctcgc cggtcagctt cgagaaatcc gcctcgagca ggaaatccac
     2041 gccgccgcgg aaatcgccgt tgatgatggc gtagcccagc agcgccacca tgagaatggc
     2101 cagcgagggc atcaggaaac gcacggcgcg ctccaggccg cgctccacgc cgcgcgccac
     2161 cacgaaaatc gtgagcgtca tgaacagcgt gtgccagaac gccagtacct tccagttgga
     2221 cagcatgccg ccgaacaggc cgttgacctc gtcggcgccg gcgccggaga agcggttcat
     2281 ggccgcgtgg ccgatgtagg acaacgacca gccggcgatt acgctgtaaa aggacaggat
     2341 gatgatgccc gcgatcacgc ccatcacgcc gaccagcgcc cagctcgggc ggctgccctc
     2401 ctcctcgccg acgatgcgca tggtcgccac gggattgcgc cggccgcgcc gtccgatgag
     2461 gatttctgcc atcatgatgg gcaggccgat cagcgccacg cacaacaggt agaacagcac
     2521 gaacgcgccg ccgccgttcg cgccggccac atacgggaac ttccatatgt tgccgagccc
     2581 gaccgccgag ccgctgaccg caaggatgaa ggccagccgg gacgaccaga ggccgtgcag
     2641 cgaggtacgc ttggccatga gggcgattac cgtgcgcttt aaaagggcgg cgattttgga
     2701 ttaaatcccc ccgcccggca acagcgggcg ccgtcgcggc ccggctttgc gacccgacca
     2761 ctccccggaa aagaacatga gcaccgccaa gaaaaaagcc gcctccggca ccgggggctc
     2821 caccatcgcc ctgaacaaga aggcgcgcca cgactatttc atcgaggaaa gcttcgaggg
     2881 cggactggtg ctggagggct gggaggtcaa gagcctgcgc gccggccgcg cccagatcgc
     2941 cgaggcctac gtcgtggtgc gcggcggtga aatctggctg gtgggcgcca acatcacgcc
     3001 gctcaatacc gcctccacgc acgtccatcc cgagcccggc cgcagccgca agctgctgct
     3061 gaaccgtcgc gagatcgacc ggttggtggg gtccgtggag cgcgccggtt acaccatggt
     3121 gccgctggcg ctgtactgga agaagggccg agccaagctg gagatcggcc tggccaaggg
     3181 caagaagcag cacgacaagc gcgccaccga gcgcgaccgc gactggcagc ggcagaaggc
     3241 gcgcatcctg aagcactaga gctgcttgaa tttgttatca ggagcttaac ggtctttctt
     3301 aaaccagttt gcgaggtgaa ctcatggtcg ccatcactcg cctgatgagc accagcccgg
     3361 tgacggtcga gccggacgat acgctcgcga ccgtgaaaga aatcttcgac cggttgaagt
     3421 ttcatcacct gcccgtggtg gaggactaca ggctggtggg gctggtatcg gaccgcgacc
     3481 tgctgaaggc cttgagcccc catctcggct cggcggccga gaccgccagg gacctggcca
     3541 ccctgaacaa gcgggttcac caggtgatgg gacgcaaccc ggtcacgctc cccgccacgg
     3601 ccaccgtctg ggacgcggtg cgggtgttca accgcgacgg tgtggcctgc atcccgatcg
     3661 tggatgaccg gcaccagccg ctcggcatac tgacctggcg ggacgtcatg ggcgccctcg
     3721 accgcgaggg ctgaccagcg cggcgacatg ttcgccgagt gccagcgagg ccgtcaggcc
     3781 cggcgagtcg ataccgaaca ggttcaccaa tccggcgatg ccgtgcgccg attcgtcctg
     3841 gacgagaaag tccgggctgt cctcgccggg accgacgagc ttgggcctga tgccgacgaa
     3901 atcgggcgac aggtcctcgt cgcggagttc gggccaccat accctgatcg cgtccgcaaa
     3961 tctcccgcgc aggctgtcgt cgaaaccata gtccatccgc tcgacgaagc gcaggtccgg
     4021 gccgaagcgg gcgcgcccgg ccaggtcgaa gccgagatgc atgccgagcg cgcccggcac
     4081 cggcagcggg tagatcagcc gcgaaaacgg gacgcggcgg tgcgcctggt agtactgccc
     4141 ggcgccgtgc caaaggcgcg gcacgtactc gggcgcgagg cccgacgtgg ccgcggccag
     4201 cgcggttgcc ccgaggcccg cagaattgac gagccaacgg cagctaagcc tttcaccatc
     4261 ggcagtctcg atctcgaaca gcccgtcgga ccgcccgatg cgcgtcacgc gcgcgtggca
     4321 gagcagcagg ccgcccgcct cttgcaggtc gcccgacagc gccatgacca gttccgccga
     4381 gtcgatgatg ccggagtcgg gagaatggat cgcggcctcg cagcgtagcg ccggctccag
     4441 cgcccgtacc gccgcgccat cgagccattc caggcggacg cggttttcga gaccgcgctc
     4501 gaacagccgg cgcaatgcgg cggcctcccc gggatgcgtc gcgacgatca gcttgccgag
     4561 ccgccgatgc ggaatgccgc gctgcgcgca gtaggcatac agcatgtcgc ggccgcggcc
     4621 gcacaacgtc gccttcagcg agccgggcgg atagtacagg ccggcgtgga ttacgccgga
     4681 gttgcggctg gtggtggctt cgcccggccg gtcctcggcc tccagcagca gcgtctcgat
     4741 gccgcagcga gccagcgccg tggccacggc caggccgacc acgccggcgc cgacgactgc
     4801 acattcgacg tccgggacgg ccacgcgtca gaccaccgcc gcggcgcgtg gcggccggaa
     4861 cgactgctgc accgccagcc cggcgacgat gagcccgagc ccggcccagg tggagggcag
     4921 gatcgcctcg ccgacgaagg cgcggatgaa gaacagcgac aggaacggcg acaggaagac
     4981 caggttgccg atgcgcgagg cgctctgcgt gagccgcagg gcactgagcc agagggcgaa
     5041 ggccagcccc atctcgaaca cgcccaccca ggccgccccg gccaggccgc gccagtccgg
     5101 caccgccagg ccgtcggtca gcgcacaggc gagcagcacg aacggcagtc ccagcgcgaa
     5161 gttgcagaac agcgccacca gggggtcgcg ctggtcgcgc gtgctgtaga tccagtacaa
     5221 cgcccacagc acggtactgc cgagtgccag cgcgacaccg accggattgg cgaactcgag
     5281 cgccagcact tcgccgcgcg tcgcaatcac ccacacgccg gcataagcca ccaggccgcc
     5341 gaccacgtcc gcgcgagtca gccgatggcc gagcagcggc acggccagga tcgccagcgt
     5401 gaacgcccag gtgtaattca gcggctgggc ctcctgcgcc ggcagccggt cataggcctc
     5461 gaacagcacg aggtagtagc acagcggatt gaccaggccg agcgcgagcg agcgcgaata
     5521 atcgcgccga ctgccgcgca gcacctcggc cagtcgcccg gtcgcgacca gcagcgcgcc
     5581 gagcgtgagc aacgagacga cgctcgccca ggcgagcaac tgcaccggcg agaggtactg
     5641 gagcgagagc ttgaacgcgg tggcgacggt ggaccagagc agcaccgcgc cgagggcgag
     5701 cgacagcgcg cgctgttcgt tgctgagcgt catcgtctct tccagccagg tcgagcacgg
     5761 cagtctagca ggcacggcgg cgcgtccgac ccgcgcgacc tgttcctgtt aaggtttgcg
     5821 cgggcccacc acaaaatcct gcgcgcactt ggcgcacgac cggagtcagt cgaataagcc
     5881 aggcaaatag ccaagccagg atactggcca cggccgcagc cttcgtcctg ctgtggaact
     5941 ccgggttcat cggcgcagcc tggggcctgc cctacgccgg caccttcacc ctgctgttct
     6001 ggcgttacct cgcgctggcg gtgttcctgg gagcggtgct cgcctggcgc cggagcctgg
     6061 cctggcccgg cctggcggcg gtcggccacg ccgccctcgt cggggtgctg gcccacggta
     6121 cctggctggc ctgcgtgctg gtgtcgctgg agcgcggcgt gcccgcggga atcgtcgccc
     6181 tggtggtggc attgcaaccg ctcgccaccg gcgccctttc cgggctggtc accggcgagt
     6241 ccaccagcgc ccggcagtgg gccggcctgg cgctgggctt cgtcggcgtc gcggtggccg
     6301 tcggcgcgcg catcgattgg tctgacaccg gctccgcctt cgcctacctt gtgccgctgg
     6361 gctcggtgat cgccatcacc gtcgcgagcc tgctgcaacg ccggcgcgag gtgctcggcc
     6421 ggcgcgacct gccgctgggc agcgccttgt tctaccagtg cgccgccacc gccctcgtct
     6481 tgttcgtgcc ggccgtggtt gtcgaaggtc tggcggcccg ctgggcgccg gagttcctgg
     6541 ccacaatggc gtggctgatc ttcgccgtct cactcggcgc ctatggcctc atgtggcggc
     6601 tgctggaaga cctggacgcc gcccgcgtgg ccagcctgtt ctacctcggc ccgccggtca
     6661 ccatgttcat gggctggctc gccttcggtg accgcctgca ggccacggac cttgccggca
     6721 tgctgatcgc catgctcggc gtaatcctgg tttatcagcg gcgcgagccg cgcaggtcca
     6781 agccgtagta tgcaatcggg atgtccggtc gcttggcgat ggtctcgttg gcgctcttga
     6841 cgcactcgaa gctcatgccg aggtgctcgg ccaccttgat cgaggccgtg ttcggcggat
     6901 cgatcaccgc taccagccgg tccaggccca tgacgtcacg ggcatgaatg atgaacggcc
     6961 tggccgcctc ggtcgcgtag ccccggcccc agtgctcctt ccagatccac cagcccagtt
     7021 cgaactcgcc gtcgtcgtgc ggctgcaggc cgaccaggcc gatgacctcc ccggtcgcca
     7081 gcagctccac ggcgccgaag cacacgccgt ggttcttcag gtggcgggcc tggcgctcca
     7141 ggatctcgtc cacttcctcg tccgcccagg tccggccttg cgtgatgtag cgcatcatgt
     7201 ccgggtcgcg caccatccgt tccagcgcgg gccggtctgc ggcccgccac ggccgaatgc
     7261 gcagccgctc ggtgacgaat tcgaagctca tgcggtgttc tcccccagca atgccgggcg
     7321 gagtatagcg gcgactagga cggcgcgcgc tccggaaccg gcgggcgccc cgcgctgcgc
     7381 ggccccgtcc agcccgcata caccagcgca agccccaccg cgagacccgc cgccgccgtg
     7441 agcagcaggg gcatgcccgc gtaggccgag aacagcgcct gccagcccgg ggtcaccgcc
     7501 agccattgga aggctgcacc ctgcagcaac gcgaccccgg ccactaccag gaacggccgg
     7561 ccgtggcgcg gcgacgaggc atacagcagc agcgccgcca cgatcgccac cgagttcgac
     7621 aggtgcaggt tggcgaaaaa cacccccatg tcctccggcc ccttgatggc gaagtacggc
     7681 acgaaatcgg tcagcagccg cgaaacgatc ggcgccagca gcagcagcgg cgtcgccagc
     7741 atgtagcggg cgtgcagctg caccttgccg cggtgacgga gcgctccgta gaacagccag
     7801 gcgaaagccg tcatcgcaat gaagtccagc agccccagcc gcgccccgaa cagcgtgtag
     7861 aacgggtgag cctgcgccac gaagcccgtc gccatcgcgt gcagcgccat caagccaccg
     7921 gccatgaaca gcgggaagag cagcaggctc gagcgcccgg ccgcccggtg cagggcgaag
     7981 cggcggttgt ggattgccca gctctgcagc accagcagca ggatccacag cgagcccgtc
     8041 accccgtgca cgtggtgggc cagtggcgag gcccccagcc gggagaaata gcccggccag
     8101 aaggccaccc cgatcgccgg gatgaggacc aggagcagcc agtgggcacg cgggtacggc
     8161 atcggggttg ctcgatgtag cgggcggttc cgtcaaaagt agccgcagca acgcccgccc
     8221 gccagcccct atgtccagtc cggcctgctg gcccgccggg aaccttcggc gtagaatccc
     8281 ggtctgacga ttacctgggg gcgacatggc ttcgacgtgt gtcgcaaaac ccggggtgca
     8341 tgccgaggtg cagggacctc gtaaaacact ttgcaaactt tagttgccaa cgacgacaac
     8401 tacgcactcg ccgcctaaaa cccggtaggg tgccgtccga ctgatagcgt gcttgtgagc
     8461 ttcaggtttc gggcgtcata actcacaaga tcgctgtgaa gcctgcccgg ggccgagcgg
     8521 ttaaatctct cgagggatcg ccgtctgttt tccctgtccg tcgggtagca gccggttaaa
     8581 acaatagacg tgaataagca tgtagacctc cgggcggagg gctcgcggac gcgggttcga
     8641 ttcccgccgc ctccaccagt ttttgtgcat taaatcaatc tcttacaact caataggccg
     8701 ctctcgaagc ggcctttttt tgtcgcgatc atcgaatgtg ttttcccagt gcggaaccgc
     8761 accgctccgc ggcggggtcc acatatccgc cattccactc accgagctcc ttctggctca
     8821 aggcgcatgg cacgttcctt tggttcgctt tcttgcagaa atgtcgcctc aaaagcgaca
     8881 cttcagcatt atcccaatct gcatgctgtc cttcgcagca agttttacat ggcgcacata
     8941 cgccgcctat tggtggtctg atgggctgga atatgaacca actgttgcag cagtggccat
     9001 ctggaacggt cgcgacactc gagcgggtgg ctttcaagat gatgcacctc gaccggtcga
     9061 aggtcgaatt cgcgtcaggc cggcgcacta tccacgctgg aggacgcctc gacaagagat
     9121 acgacctggt cgtcgccgac gctgggccag gcgaggcact ggcttcgtga tcgcctcggc
     9181 agccgtatat ctgggtgccg cggcagccga gatcgcgggt tgcttcgcct tctgggcctg
     9241 gttacggcta gacaaatctc ccgcctggct gattcccggc atgatcagcc tggcggcgtt
     9301 cgcgtttctg ctgacgcgaa tcgacagcga cttcgcgggc cgtgccttcg ctgcctaggc
     9361 ggcgtctaca tcgcttcgtc gctcgcgtgg ctgtggttgg tagaggatca gacgcccgat
     9421 cgctgggatg tcatcggcgg attgatctgc ctattcggcg ccgccgtgat tctttgggga
     9481 ccaagacccg cctgagacgc caggcggcca atgctgggcc caccgccggt gcttcagttc
     9541 gagtgccctg aggcatcgtg ccccgttcca cccgaatcac aaaagcggcc gacatcctgg
     9601 tgcgcacgat cgacgtgctc aaattcgagg ctggaatgtg aaatgtcgaa gcggttcttc
     9661 agcgtttcct tgatgtcact cttaatcgcc tcctggcgtc cccatccgat atcggtcaga
     9721 acgacatggc agtccaaagc ggcctcgtgt tcctgcattt gccatagatg aacatgatga
     9781 atccccgata cctcatcgat agtgcacagc tctctgatca gtatgtcagc gtcaatcgct
     9841 ggagggctcc cgagcatcag cgtgcggatt ggtccgccta tctcgttgat gctcaggtac
     9901 aggatatagc ctgcgatcgc gagggtcacg atcgggtcca ccacccacca gtcgtacaaa
     9961 agaatcaacg tgccacctgc cactacaccg acggaggcca aggcatccga caggttgtgg
    10021 aggaaaagcg cccgaatgtt catgctgccc ttttgcatcg accaggtcag tagcgctgtc
    10081 agggtatcga ccaaaagcgc caccgtgcca actaccacca cggtccagcc agcaacctct
    10141 ggcgggtcta tcaaacgcaa ggccccttcg tagacgagat agaccccgac aataatcagc
    10201 gtcgtgtaat tgatcagcgc ggccacgact tcgatccggg cgtagccgaa ggtcatgcgg
    10261 ctgtcggcgg gtcgccgcgc aatccggcgc gccgcatagg cgattaccag cgaggccata
    10321 tcggaaaagt tgtgcagcgc gtcggcgatc agggccaggc tgcccgaaag cacacc
//
LOCUS       DAOMPG010000068        10372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2764_length_10372_cov_3.753126, whole genome shotgun sequence.
ACCESSION   DAOMPG010000068 DAOMPG010000000
VERSION     DAOMPG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_10372_cov_3.753126"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLS34_05280"
     CDS             <1..775
                     /locus_tag="PLS34_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRP86916.1"
                     /translation="GFLGMLHMEIVQERLEREYKLNLITTAPTVVYQVLQTDGKIVEV
                     DNPSKLPPVNLVAEIREPIINASILVPHAHVGAVIKLCIEKRGVQKRLHYTGSQVQVE
                     YDLPLSEVVLDFFDRLKSCSRGFASFDYRLDRYQAAPLTKLDILVNGDRVDALSIITH
                     REAAQRRGREVVEKMRELIPRQMFEVAIQAAIGGQIVARSSVKALRKNVTAKCYGGDI
                     TRKRKLLEKQKEGKKRMKQVGNVEIPQEAFLSVLQVERK"
     gene            848..1609
                     /gene="lepB"
                     /locus_tag="PLS34_05285"
     CDS             848..1609
                     /gene="lepB"
                     /locus_tag="PLS34_05285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRP86917.1"
                     /translation="MGFIWALDALVLRPRRLGAFEAGAEGGDSPKEPALIEFSRSFFP
                     IVLVVLLIRSFAYEPFRIPSSSMMPTLRVGDFIFVSKFSYGLRLPVLNTKILSLGEPE
                     RGDVAVFRLPSDPSTNYIKRIVGVPGDVVSLRDKQLWINGEPVPLEDTGESVEIECGG
                     FWFSARRLTESIGAGSHSIFLGEQRDRSSPFLDRPVPEGQYLVMGDNRDCSRDGRIIG
                     YIPEGHLVGRAVRIWMNWDAESSRPLWHRIGDRIE"
     gene            1660..2022
                     /locus_tag="PLS34_05290"
     CDS             1660..2022
                     /locus_tag="PLS34_05290"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HRP86918.1"
                     /translation="MRNQQGMTLIGMVIVAFIAGIFVLAALRLTPIYLEYMNVDSALK
                     SMKRDLDGSNAGPAQIRTAMARRFDIDNVKSLNARDIKIERTANGYNVAAQYESRAHF
                     VANVSFVVNFDKSVEVAR"
     gene            2019..2693
                     /gene="rnc"
                     /locus_tag="PLS34_05295"
     CDS             2019..2693
                     /gene="rnc"
                     /locus_tag="PLS34_05295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRP86919.1"
                     /translation="MTDDRGWLERELGYRFKDEDLLARALTHRSAGGRHNERLEFLGD
                     AALSLVVAEALYQRLPDAPEGHLSRLRASLVRRSSLAEVARGLGFPGRLRLGPGELKS
                     GGFRRDSILADALEAVLGAIYLDGGLDALRTAALHLYGDRLDDLPAHEDLKDPKTLLQ
                     ERLQARGLPLPTYRLEAVAGEDHRQQFTVSCEIEGLADGASGTGGSRRAAEQAAAARV
                     LAQLDG"
     gene            2686..3588
                     /gene="era"
                     /locus_tag="PLS34_05300"
     CDS             2686..3588
                     /gene="era"
                     /locus_tag="PLS34_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRP86920.1"
                     /translation="MAEPVFRCGFVAVVGRPNVGKSTLVNALVGRKVSIVTSRPQTTR
                     QRVVGVANAPAAQLLFVDTPGMHRRERRELNKLMNRSAQASLADADVVLVVVEAGRWV
                     EEDDDVLARALASGRPVMLALNKIDRIRPREAVLPLLQEAAARAEFAAVVPISAERGE
                     NLDRLRQLLEQAVPEGPALFPPEQWSNRDERFHIAEIVREKLMRRLREELPYGLAVEV
                     ERLAESEQGRLEIGAVIWVEREAHKAIVIGKGGEMLKAAGQAARLELKSRFNRPVHLE
                     LWVKVREHWTDRESDLRRLGYDGD"
     gene            3594..4304
                     /gene="recO"
                     /locus_tag="PLS34_05305"
     CDS             3594..4304
                     /gene="recO"
                     /locus_tag="PLS34_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRP86921.1"
                     /translation="MLRVELEPAFILHPRPFRDTSLLLEVFSRNHGRVGLVARGARGP
                     RSRLRGVLQPFRPLLVSWQLRGELGTLTAAETAGRAAAAPVGGDALLGLYYLNELLLR
                     LTARLDPHAELYDAYAAALAAITGGAPLAPALRRFERRLLDALGYGPELARDAAGRPL
                     EAAASYRYDPALGLASCRPDAAGALLGASVLALAEDRLVDPRALADARRLLRAALDAH
                     LDGRPLKSREMLSRLRRG"
     gene            4352..5089
                     /gene="pdxJ"
                     /locus_tag="PLS34_05310"
     CDS             4352..5089
                     /gene="pdxJ"
                     /locus_tag="PLS34_05310"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRP86922.1"
                     /translation="MKNTIALGVNIDHVATIRQARRTRYPDPVYAALMAEQAGADSIT
                     VHLREDRRHIQDRDLRAMKDVLQTRMNLEMAVTDEMLAIATDIRPRDVCLVPERREEL
                     TTEGGLDVAGQLERVRDAVGRLSAAGIRVSLFIDPEVRQLEASVAAGAPVVELHTGAY
                     ADAPEERRQRAELERVRAAAEACAVLGLAVNAGHGLNYHNVQPIARIRGIMELNIGHA
                     IVARAIFDGLSTAVIEMKRLMTEARLQ"
     gene            5086..5472
                     /gene="acpS"
                     /locus_tag="PLS34_05315"
     CDS             5086..5472
                     /gene="acpS"
                     /locus_tag="PLS34_05315"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRP86923.1"
                     /translation="MIFGIGTDVVRLDRVEATLERFGEHFVRRLLLPEEEAQYRRNGR
                     KARFLAMRFAAKEAVVKAMGTGFRNGMWIRDVGIVANPLGKPEIIYSERGRALCRQMG
                     VGEGHVSLTDEAGLVVAFAVLLRATQ"
     gene            5469..6242
                     /locus_tag="PLS34_05320"
     CDS             5469..6242
                     /locus_tag="PLS34_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP86924.1"
                     /translation="MTTLAFDIETVPDVDFGRRHLGLDGLADEDVAQAMFALRRARTG
                     SDFLPLHQHRVVAIACVMRSREGLKLWSLGDESADEAELVTRFFDGIERYCPELVSWN
                     GSGFDLPVLHYRALRHGVAAPRYWEVGDADRDFRYNNYLGRFHWRHIDLMDVLAGYQL
                     RGAARLDEVAVLLGLPGKLGLSGDRVWDEWRQGRLGAVRDYCETDVLNTWLIYLQFEQ
                     LRGRRNAKEIAAEQQLVVDTLRADGRPHLAEFLAAWGRA"
     gene            6239..6791
                     /locus_tag="PLS34_05325"
                     /pseudo
     CDS             6239..6791
                     /locus_tag="PLS34_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511296.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
     assembly_gap    6792..6869
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6870..7556
                     /locus_tag="PLS34_05330"
     CDS             <6870..7556
                     /locus_tag="PLS34_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRP86925.1"
                     /translation="PADLAALERFGAAHGVDVWLQPGGLETLAPLLADTPRPSYRLAG
                     FDLEIGFLPTDFVQVNAAINERAVARAVELLDPLPGERVVDLFCGLGNFSLALARRGA
                     RVCGVEGDAGLVERARENAASNRLAAEFHVANLFEDCRALPWAREPCAALLLDPPRAG
                     AEASIRLVPVLRPARIVYVSCHPGTLARDAGILVHEYGYRLSGAGVMDMFPHTAHVES
                     IALFERDGGA"
     gene            7566..8063
                     /locus_tag="PLS34_05335"
     CDS             7566..8063
                     /locus_tag="PLS34_05335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRP86926.1"
                     /translation="MQSVARIDIDLGAQRLALLRPGQAPRHWPVSTARNGAGEQRDSE
                     CTPRGRHVVRARIGAGLPAGTVFVGRRPTGELWSPELARRHPGRDWILTRILWLSGLE
                     PGRNRLGDVDTMRRYIYIHGTPDDQPVGAPASHGCIRMRNADIVELFELVPAGTEVVI
                     REGAA"
     gene            8060..9106
                     /gene="nagZ"
                     /locus_tag="PLS34_05340"
     CDS             8060..9106
                     /gene="nagZ"
                     /locus_tag="PLS34_05340"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRP86927.1"
                     /translation="MTLGPVMLDIEGAVLAPEDRELLAHPNVGGVILFSRNYQSPEQL
                     KELTREIHALRQPELVIAVDQEGGRIQRFRDGFTPLPPMHMLGRQFELDRERALQVAE
                     TCGWLMAAELRAVGVDVSFAPVLDLDYGLSEVIGDRALHRDPEVVARLAGRFAAGMRH
                     AGMGAVGKHFPGHGGVTADSHHALPEDRRPYADLLDDMRPYERLIAEGLAGVMAAHIV
                     YARLDRLPAGFSRWWLGTELRIRLRFQGAIFSDDLSMAGAAFAGQPEERARLALEAGC
                     DVVLVCNDRPAAVRVVEALGDWYEPAAHLRLTRLRGRGHYDAPLQASPEWQRAEALVR
                     EFEDGRPRLVLEGD"
     gene            9106..9666
                     /locus_tag="PLS34_05345"
     CDS             9106..9666
                     /locus_tag="PLS34_05345"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HRP86928.1"
                     /translation="MQEDFNAVLARADRIAGRVEVESALDRMAGEISRDLAGRDPLVL
                     SVMLGGLMPTAWLLQRLAFPLQLDYVHASRYRGGTRGGATIDWIARPRAPLAGRTVLV
                     VDDILDEGHTLAAILEDCRRQGAAQVRSAVLVEKLHDRRVPGLRADYTGLRIEDRYLF
                     GCGMDYHEHHRQHAAVYALAEEDERA"
     gene            9663..>10372
                     /locus_tag="PLS34_05350"
     CDS             9663..>10372
                     /locus_tag="PLS34_05350"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HRP86929.1"
                     /translation="MSLLGIIGGSGLTSFEDLQLERRSTGSVRWGRPSGPLLHGRVAG
                     RPVVFMARHGSDHALAPHEVNYRANVWALHEAGARRVIAVNAVGGIGERFRPGVLVVP
                     HQLIDYSWGRAHTFCGEQGEPLQHIDFTEPYSALMRALLLAGAARAGLEAVDGGVYAV
                     MQGPRLETAAEIDRLERDGCDLVGMTGMPEASLARELGMASSAIYRYFASRDELITAL
                     VIDAYDDLGAAAEAGLTAA"
BASE COUNT         1480 a         3476 c         3816 g         1522 t
ORIGIN      
        1 cggcttcctc ggcatgctgc acatggagat cgtgcaggag cggctggagc gcgaatacaa
       61 gctgaacctg atcaccacgg cgccgaccgt ggtctaccag gtgctgcaga ccgacggcaa
      121 gatcgtcgag gtggacaatc cctccaagct gccgccggtc aatctcgtcg ccgagatccg
      181 cgagccgatc atcaacgcca gcatcctggt gccgcacgcg cacgtcggcg ccgtcatcaa
      241 gctgtgcatc gagaagcgcg gggtgcagaa gcgcctgcac tacaccggca gccaggtcca
      301 ggtcgagtac gacctgccgt tgtcagaggt ggtgctggat ttcttcgacc ggctgaagtc
      361 ctgcagccgc ggcttcgctt ccttcgacta ccggctggac cgctaccagg cggcgccgct
      421 gaccaagctg gacattctcg tcaacggcga tcgcgtcgac gcactgtcga tcatcaccca
      481 tcgcgaggcg gcccagcggc gcggccgcga ggtggtcgaa aagatgcgcg aactgattcc
      541 gcggcagatg ttcgaggtcg ccatccaggc ggcgatcggc gggcagatcg tggcgcgcag
      601 cagcgtcaag gcgctgcgca agaacgtcac ggccaagtgc tacggcggcg acatcacgcg
      661 caagcgcaag ctgctggaga agcagaagga aggcaagaag cggatgaagc aggtcggcaa
      721 cgtggaaatc ccgcaagagg ccttcctgtc ggtcttgcag gtggaacgca aataacgaat
      781 acttcgcgcg aagaatcggg ggcaacattt ggatttttct ctaattctgg ttctgctgac
      841 atttgcgatg ggcttcatct gggcgctcga cgcgctggtg ttgcggccgc gtcgcctggg
      901 cgcgttcgag gccggggcgg aaggtggcga cagcccgaag gagccggcgc tgatcgagtt
      961 cagccgctcc ttcttcccca tcgtgctggt ggtgctgctg atccgctcct tcgcctacga
     1021 gccgttccgc atcccctcga gctcgatgat gcccaccctg cgggtcggcg atttcatctt
     1081 cgtcagcaag ttcagctacg ggctgcgcct gccggtgctc aataccaaga tcctgtccct
     1141 gggtgagccg gaacggggcg acgtggcggt gttccggctg ccctccgacc cgtccaccaa
     1201 ctacatcaag cgcatcgtcg gcgtgccggg cgacgtcgta tcgctgcgcg acaagcagct
     1261 gtggatcaac ggcgagccgg tgccgctcga ggacacgggc gagagcgtcg agatcgagtg
     1321 cggcggcttc tggttcagcg cccggcgcct caccgagagc atcggggcgg gcagccacag
     1381 catattcctc ggcgaacagc gcgatcgcag cagcccgttc ctcgaccggc cggtgcccga
     1441 gggccagtac ctggtgatgg gcgacaatcg cgattgcagc cgggacgggc gcatcatcgg
     1501 ttatattccg gaaggccacc tggtgggccg ggcggtacgc atctggatga actgggacgc
     1561 ggagtccagc cggccgctgt ggcacaggat cggggaccgc atcgaatgat cgggcggctt
     1621 tcaggcatca tgcatcgagc ctcgcgaggg ggacgcacta tgcgcaatca gcagggaatg
     1681 accctgatcg gaatggtcat cgtcgcgttc atcgccggca tcttcgtgct ggcggcgctg
     1741 cgcctgacgc cgatctacct tgagtacatg aacgtcgact cggccctgaa gtccatgaaa
     1801 cgggatctcg acggcagcaa cgccgggccg gcgcagatcc gcaccgccat ggcgcggcgc
     1861 ttcgacatcg acaacgtaaa gagcctgaac gcgcgcgaca tcaagatcga gcgcaccgcc
     1921 aacggctaca acgtggcggc ccagtacgag agccgggcgc atttcgtggc caacgtctcc
     1981 ttcgtggtga acttcgacaa gtcggtcgag gtcgcgcgtt gaccgatgac cgcgggtggc
     2041 tggagcgcga gctcggctac cgcttcaagg acgaagacct gctggctcgc gcgctgaccc
     2101 accgcagtgc gggcgggcgg cacaacgagc gtctcgagtt tctcggcgat gccgcactca
     2161 gcctggtggt cgcggaggcg ctgtaccagc gcctgccgga tgcgcccgag ggccacctca
     2221 gccggctccg cgccagcctg gtgcggcgct cctcgctggc ggaagtggcc cgcgggctgg
     2281 gctttcccgg gcgcctgcgg ctgggccccg gcgagttgaa gagcggcggg ttccggcgtg
     2341 actccatcct ggccgacgcg ctcgaagccg tgctcggggc gatctacctc gacggcgggc
     2401 tcgacgccct gcgaacggcg gcgctgcacc tgtacggcga ccggctggac gacctgccgg
     2461 cccacgagga tctcaaggat cccaagaccc tgctgcagga acggctgcag gcccgcggcc
     2521 tgccgctgcc gacctatcgc ctggaggcgg tggcgggcga agatcaccgc cagcagttca
     2581 cggtctcctg cgagatcgag ggcctggccg acggcgcgag cggcaccggc ggcagccggc
     2641 gggcggcgga gcaggccgcg gcggcccgcg tactggcgca actcgatggc tgagccggtg
     2701 ttccgctgcg gcttcgtcgc cgtcgtcggc cggcccaacg tcggcaagtc gacgctggtc
     2761 aatgcgctgg tcgggcgcaa ggtgagcatc gtcaccagcc gcccgcagac cacccggcaa
     2821 cgggtcgtcg gcgtggccaa cgcgcccgcc gcgcagctgc tgttcgtgga cacccccggc
     2881 atgcaccgtc gcgagcgacg cgagctcaac aagctcatga accgcagcgc gcaggcctcg
     2941 ctggccgatg ccgacgtggt gctcgtggtg gtcgaggccg ggcggtgggt ggaagaggac
     3001 gacgacgtgc tggcgcgcgc gctggcctcc gggcggccgg taatgctggc gctcaacaag
     3061 atcgaccgca tccgtccgcg cgaggccgtg ctgcccctgc tgcaggaagc ggcggcgcgc
     3121 gccgagttcg ccgcggtggt gccgatctcg gccgagcggg gcgagaacct ggaccggctg
     3181 cgccagctgc tcgagcaggc ggttcccgag ggtccggcgc tgttcccgcc ggagcaatgg
     3241 agcaatcggg acgagcgctt ccacatcgcc gagatcgtgc gcgagaagct gatgcgccgg
     3301 ctgcgcgagg agctgcccta cggcctggcg gtggaggtcg agcggctggc ggagagcgag
     3361 cagggacggc tggagatcgg cgccgtcatc tgggtcgagc gcgaggccca caaggcgatc
     3421 gtgatcggca agggcggcga gatgctcaag gccgcgggac aggccgcgcg actggagctg
     3481 aagtcgcgct tcaaccggcc ggtgcacctg gagctctggg tgaaagttcg cgagcactgg
     3541 accgaccgcg agtccgacct gaggcgcctc ggctacgacg gcgactgacg cccttgctgc
     3601 gcgtcgagct cgagcctgcc ttcatcctgc atccccggcc cttccgggac accagcctgc
     3661 tgctggaggt gttcagtcgc aaccacggcc gcgtcgggct ggtggcgcgc ggcgcgcgcg
     3721 gcccgcgcag ccggctgcgc ggcgtgctgc agcccttccg gcccttgctg gtgtcctggc
     3781 agctgcgcgg cgagctgggc acgctgaccg cggcggaaac ggccggccgc gccgccgcgg
     3841 cgccggtcgg cggcgatgca ctgctcgggc tgtattacct gaacgagctg ctgctgcgcc
     3901 tgaccgcgcg gctggacccc catgcggagc tttacgatgc ctacgcggcg gcactcgccg
     3961 ccattaccgg cggcgcgccg ctggcgccgg cgctgcgccg tttcgagcgg cgcctgctgg
     4021 atgcgctcgg gtatggcccc gagctggccc gcgatgccgc cggccggccg ctcgaggcgg
     4081 cggccagcta tcgctacgat ccggcgctcg gcctggcgag ctgccggccc gatgcggccg
     4141 gcgcgctgct cggcgcctcg gtgctggcgc tggcggagga ccggctggtc gatccgcggg
     4201 cgctggcgga tgcccgccgg ctgctgcgcg ccgcactgga tgcccacctc gacgggcggc
     4261 cgctgaagag ccgggagatg ttgagccgcc tgcgccgcgg ctgacatggc aaaatggcca
     4321 cagcgcaggg caggcacagc agggatccgt gatgaagaac accatcgcac tgggggtcaa
     4381 catcgaccac gtcgcgacca tccgccaggc gcggcggacg cgctatcccg accccgtcta
     4441 cgcggcgctg atggccgagc aggccggcgc ggacagcatc acggtgcacc tgcgcgagga
     4501 tcgccggcac atccaggatc gcgacctgcg cgccatgaag gacgtgctgc agacccgcat
     4561 gaacctcgaa atggcggtca ccgacgagat gctggccatc gccaccgaca tccggccgcg
     4621 ggacgtctgc ctggtgccgg aacgacgcga ggagctgacc accgaaggcg ggctggacgt
     4681 ggccggtcag ctcgagcggg tgcgtgacgc ggtgggccgc ctcagcgcgg cgggcatccg
     4741 ggtgtcgttg ttcatcgacc ccgaagtgcg gcagctggag gcgtcggtgg cagcgggcgc
     4801 gccggtggtg gaactgcaca ccggcgccta tgccgacgcg ccggaagagc ggcgccagcg
     4861 cgccgagctg gagcgcgtgc gcgccgcggc ggaggcctgc gcggtgctcg gcctggccgt
     4921 gaatgccggc cacggcctga attaccacaa cgtgcagccg atcgcgcgca tccgtggaat
     4981 catggagctg aacatcggcc acgccatcgt ggcgcgggcg atcttcgacg gcctgtcgac
     5041 cgccgtgatc gagatgaagc gcctgatgac cgaggcgcgg ctgcagtgat tttcggcatc
     5101 ggcaccgacg tcgtgcggct ggatcgcgtc gaggcgacgc tggagcgctt cggggaacac
     5161 ttcgtgcggc gcctgctgct gccggaggaa gaggcgcagt atcgtcgcaa cggtcgcaag
     5221 gcacgcttcc tggcgatgcg cttcgccgcc aaggaggcgg tggtgaaggc gatggggacc
     5281 ggctttcgca acggcatgtg gatccgcgac gtgggcatcg tcgccaatcc gctcggcaag
     5341 ccggagatca tctactcgga acgcggccgc gcactgtgcc ggcagatggg cgtcggcgag
     5401 ggccacgtca gcctcacgga cgaggccggg ctggtggtgg cgttcgccgt gctgttgagg
     5461 gccacgcagt gaccacgctg gcgttcgaca tcgagacggt gcccgacgtg gacttcggac
     5521 ggcgtcacct gggactcgac gggctggcgg acgaggacgt cgcgcaggcc atgttcgcgc
     5581 tgcgccgggc gcgcaccggc agcgacttcc tgccgctgca ccagcatcgc gtggtcgcga
     5641 tcgcctgcgt catgcgctcg cgcgagggcc tcaagttgtg gtcgctgggc gacgagtcgg
     5701 ccgacgaggc cgagctggtg acgcgctttt tcgacggtat cgagcgctac tgcccggaac
     5761 tggtgtcctg gaacggctcg ggtttcgacc tgccggtgct gcactatcgc gcgctgcgcc
     5821 acggcgtggc ggcgccgcgc tactgggagg tcggcgacgc cgatcgcgac ttccgctaca
     5881 acaattacct cggccgcttc cactggcgcc acatcgacct gatggacgtg ctggcgggct
     5941 accagctgcg cggcgcggcg cgcctcgacg aggtcgccgt gctgctcggg ctgcccggca
     6001 agctcgggtt gagcggtgac cgtgtctggg acgaatggcg ccaggggcgg ctcggcgccg
     6061 tgcgcgatta ctgcgagacc gacgtgctga acacctggct catctacctg caattcgagc
     6121 agctgcgcgg ccggcgcaac gccaaggaaa tagctgccga gcagcagctg gtggtggaca
     6181 ccctgcgcgc cgacggccgg ccgcatctcg ccgagttcct ggcggcctgg ggccgcgcat
     6241 gagccgcaag ccggatcccg cgcccttcga ggcgcgggtg atcgacctgt cgcacgacgg
     6301 ccgaggcgtg gcccggctcg acggcaagac ggtgttcatc agcgacgcgc tgcctggcga
     6361 gcgggtgctg gcgagccgcg tcagccggcg gcgcagccac gacgacgctg tgctcgtcga
     6421 agtgctcgag gcctcggcgg accgcgttac gccgcgctgc ccccatttcg gcgtgtgcgg
     6481 cggctgcagc ctgcagcaca tggcgccggc ggcgcagctg gccgccaagg agcgcaccct
     6541 gcacgacaac ctcgagcgcc tcggccgggt cgcggcggcg acgcgctgga cgccgattcc
     6601 cgggccggcc tggggctatc ggcggcgcgc gcgcctcggt gcgcggaagg tggacaagaa
     6661 gggccgcgtg ctggtcggct ttcgcgagcg gcgcgcgccg ctggtcacgg ccatggacag
     6721 ctgcgcggtg ctggcggccg gcgtcggcga cctggtgccg gcgctggcgc aactgatcgg
     6781 cgggctgagc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccgcggacct ggccgcgctg gagcgctttg
     6901 gcgccgcaca cggcgtcgac gtctggttgc agcccggcgg tctggagacg ctggcgccgt
     6961 tgcttgccga cacgccgcgc ccgagctatc gcctggccgg cttcgatctc gagatcgggt
     7021 tcctgccgac cgacttcgtc caggtcaacg ccgccatcaa cgagcgcgcc gtggcgcgtg
     7081 ccgtcgagtt gctggatccg ctgcccggcg agcgcgtcgt cgacctgttc tgcggcctgg
     7141 gcaatttcag cctggcgctg gcgcggcgcg gcgcgcgggt gtgcggcgtc gagggcgacg
     7201 ccgggctggt cgagcgggcg cgcgagaacg cggcaagcaa ccgcctggcg gcggagttcc
     7261 acgtcgccaa cctgttcgag gactgccggg cgctgccctg ggcccgcgag ccctgcgcgg
     7321 cgctgctgct ggacccgccg cgggccgggg cggaagcctc catccggctg gtgcccgtgc
     7381 tgcgcccggc gcgcatcgtg tatgtttcct gtcacccggg gacgctcgcg cgcgatgcgg
     7441 gtatcctcgt gcacgagtac ggctaccgcc tcagcggcgc gggggtcatg gacatgtttc
     7501 cgcataccgc ccacgtggaa tccatcgccc tgttcgagcg ggacggcggc gcctgatcca
     7561 ctgacatgca gtccgtcgcc cgcatcgaca tcgatctcgg cgcccagcgc ctcgcgctgc
     7621 tgcgccccgg gcaggcgccg cgtcactggc cggtgtccac ggcccgcaac ggcgccggcg
     7681 agcagcgcga cagcgagtgc acgccgcgcg gccggcacgt ggtgcgcgcg cgcatcggcg
     7741 ccggcctgcc ggccggcacg gtgttcgtcg gccggcggcc caccggcgag ctctggtcgc
     7801 ccgagctggc gcgccggcac cccggccggg actggatcct gacgcgcatc ctctggctga
     7861 gcgggctcga gcccgggcgc aaccggctcg gcgacgtcga caccatgcgc cgctacatct
     7921 acattcacgg cacgccggac gaccagccgg tgggggcccc ggcgtcgcac ggctgcattc
     7981 gcatgcgcaa cgcggacatc gtcgagctgt tcgagctcgt gcccgccggc accgaggtcg
     8041 tgatcaggga gggcgcggca tgacgctcgg tccggtgatg ctcgacatcg agggcgccgt
     8101 gctggcgccg gaggatcgcg agctgctggc gcacccgaac gtcggcggcg tgatcctgtt
     8161 cagccgcaac taccagtcgc cggagcagct caaggagctc acccgcgaga ttcatgcact
     8221 gcggcagccc gagctggtga tcgcggtgga ccaggaaggc gggcggatac agcgcttccg
     8281 cgacggcttc accccgctgc cgccgatgca catgctcggc cgccagttcg agctggatcg
     8341 tgagcgcgcg ctgcaggtag ccgagacctg cggctggctg atggcggcgg aactgcgcgc
     8401 cgtcggggtg gacgtgagtt tcgcgccggt gctggatctc gactacggcc tgagcgaggt
     8461 gatcggcgac cgggccctgc atcgcgatcc ggaagtcgtg gcgcggctgg cgggccgctt
     8521 tgcggcgggc atgcggcacg cgggcatggg cgcggtcggc aagcattttc ccggccacgg
     8581 cggcgtgacc gccgattcac atcatgccct gccggaggac cggcggccgt acgcggacct
     8641 gctcgacgac atgcggcctt acgagcggct gatcgccgag gggctggcgg gcgtgatggc
     8701 tgcgcacatc gtctacgcgc gcctcgatcg gctgccggcg ggcttttccc gctggtggct
     8761 cggcaccgag ctgcgcatcc ggctgcgctt ccagggcgcc atcttttccg acgacctgtc
     8821 gatggcgggc gccgcgttcg ccggccagcc cgaggagcgc gcacggctgg cgctggaggc
     8881 cggttgcgac gtggtgctgg tgtgcaacga tcggccggcg gcggtgcggg tggtggaggc
     8941 gctcggcgac tggtacgagc cggccgctca tttacggctc acccggctgc gcggccgcgg
     9001 ccactacgac gcaccgctgc aggccagccc cgagtggcag cgtgccgagg cgctggtgcg
     9061 cgaattcgag gacggccggc cgcggctggt gctggaggga gactgatgca ggaggatttc
     9121 aacgccgtcc tggcgcgcgc cgaccggatc gcgggccgcg tcgaggtcga gtcggcgctc
     9181 gaccggatgg ccggcgagat cagccgggac ctggcggggc gtgatccgct ggtattgtcg
     9241 gtgatgcttg gcggactgat gcccacggcc tggctgctgc agcggctggc gttcccgctg
     9301 caactggact acgtgcacgc cagccgctac cggggcggga cgcgcggcgg cgcgaccatc
     9361 gactggatcg cccggccgcg ggcaccgctg gcggggcgca cggtgctggt ggtggacgat
     9421 atcctcgacg agggccatac gctggcggcc atcctcgagg actgccgccg ccagggcgcg
     9481 gcgcaggtgc gcagcgcggt gctggtggaa aaactgcatg accggcgcgt gcccggcctg
     9541 cgggccgact acaccggctt gcgcatcgag gaccgctacc tgttcggctg cggcatggat
     9601 tatcacgagc accacaggca acatgcggcc gtttacgccc tggcggagga ggacgagcgc
     9661 gcgtgagcct gctgggcatc atcggcggca gcggcctgac cagcttcgag gacctgcagc
     9721 tcgagcggcg cagcaccggc agcgtgcgct ggggccggcc gtcgggcccg ctgctgcatg
     9781 gccgcgtggc gggtcgtccg gtggtcttca tggcgcgcca cggcagcgac cacgcgctgg
     9841 ccccgcacga ggtcaactat cgcgccaatg tctgggcgct gcacgaggcc ggcgcgcgcc
     9901 gcgtgatcgc ggtgaacgcg gtcggcggca tcggcgagcg attccgcccg ggcgtcctgg
     9961 tggtgccgca ccagttgatc gattacagct ggggacgggc acacactttc tgcggggagc
    10021 agggggagcc gctgcagcac atcgatttca ctgagcccta cagcgcgctc atgcgcgcgc
    10081 tgctgctcgc gggggcggcg cgtgccgggc tcgaggccgt cgacggcggg gtctatgccg
    10141 tgatgcaggg gccgcgcctg gagaccgccg cggagatcga ccggctggag cgcgacggct
    10201 gcgacctggt cggcatgacc ggcatgccgg aagcgagcct ggcccgcgag ctcggcatgg
    10261 cgtcatcggc gatctaccgc tactttgcca gccgcgacga gctgatcacg gcgctggtca
    10321 tcgacgccta tgacgatctc ggcgccgcag cggaagccgg cctcaccgcg gc
//
LOCUS       DAOMPG010000069        10272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2811_length_10272_cov_4.557600, whole genome shotgun sequence.
ACCESSION   DAOMPG010000069 DAOMPG010000000
VERSION     DAOMPG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_10272_cov_4.557600"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /gene="pgi"
                     /locus_tag="PLS34_05355"
     CDS             complement(<1..234)
                     /gene="pgi"
                     /locus_tag="PLS34_05355"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRP86930.1"
                     /translation="MTSKAVNWPRGLKAWRALKEHYREDIAKRHLRAVFRRDPERYTR
                     FSVEAEGLLLEYSRNRVSKKTLRLLQRLAEETGV"
     gene            complement(272..1288)
                     /locus_tag="PLS34_05360"
     CDS             complement(272..1288)
                     /locus_tag="PLS34_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRP86931.1"
                     /translation="MKVLVTGAAGFIGFHTAKKLLERGDEVVGLDNLNEYYDPTLKRA
                     RLSRLQDKRNWRFRKLDLVDRSGMMELFKSEKFDRVVHLAAQAGVRYSIENPMAYVES
                     NLVGQTNLLEGCRHAEVEHLVYASTSSVYGANTKMPFSVHQNVDHPLSFYAATKKANE
                     LMAHTYSALYQLPTTGLRFFTVYGPWGRPDMALFKFTKAILEGQPIEVFNYGHHRRDF
                     TYIDDIVEGVVRCLDKVAAPNPAWDSNAPDPGTSFAPYRLYNIGNQGPIELMRYIEVL
                     EERLGKKAEKELLPMQPGDVPDTWADVEALVEDVGYRPQTPVEEGIARFVDWYLSYYR
                     NDQR"
     gene            complement(1358..2626)
                     /gene="tviB"
                     /locus_tag="PLS34_05365"
                     /pseudo
     CDS             complement(1358..2626)
                     /gene="tviB"
                     /locus_tag="PLS34_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
     gene            2869..3402
                     /locus_tag="PLS34_05370"
     CDS             2869..3402
                     /locus_tag="PLS34_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRP86932.1"
                     /translation="MNKRILNLLLMGLMLAAVWLLLSGLFKPLLVGLGMAATLLTLWL
                     ASRMGIIDAATHPVWAVFRFVSFWPWLTVEIIKSSIDVARRVLSPSMPISPTVFELRS
                     TQKTVVGRVVLANSITLTPGTVTLDVDGDRLTVHALASESVEYALSGEMDRRVSRAEG
                     PPADGAAPPGGRAGDAA"
     gene            3399..3674
                     /locus_tag="PLS34_05375"
     CDS             3399..3674
                     /locus_tag="PLS34_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRP86933.1"
                     /translation="MIAAAILSIMVTMALAVIRALLGKTVYDRILAVNVFGTKTVLLI
                     AALAFLTGRPDVLDIALAYALINFIGMIAVLRYFEYGGAEPAGRESD"
     gene            3674..4003
                     /gene="mnhG"
                     /locus_tag="PLS34_05380"
     CDS             3674..4003
                     /gene="mnhG"
                     /locus_tag="PLS34_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRP86934.1"
                     /translation="MALAVDLLSWALILIGGAFGIIGGIGLLRLPDFFSRIHAASLTD
                     SMCAPCIIAGLMLQSGFTLVTVKLGFLIVFLFLTSPTASHALAKAALRGGVESGQRHP
                     GPQEERR"
     gene            4003..5007
                     /locus_tag="PLS34_05385"
     CDS             4003..5007
                     /locus_tag="PLS34_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRP86935.1"
                     /translation="MDTLVDILLLAFLAICAIAILRLRDLFAMAMMFGLFSLISAGLF
                     TTMDAPDVAFTEAAVGAGISTILILATLAQTARREQRQSGQPRLVPLLVVWITGAVLI
                     YGTADLPAFGDPAAPAHNHVAPHYLQESPNEIGIPNIVAAVLASYRGFDTLGEVIVVF
                     AAVVGVISLIGLRRRPEGVKPNAAALSQHAVLQVIAKLVVPVILLFGLYVQFHGEYSP
                     GGGFQAGVLFSAGIILYALVFGLDAARSVIPPKVLIALPGIGALIFGGVGVVTLLLGG
                     NYLDYDQLGADPVAAQHLGILLVELGVGVTVASVMLLIYFTFAERGRGKRADDGEAAE
                     "
     gene            5004..5357
                     /locus_tag="PLS34_05390"
     CDS             5004..5357
                     /locus_tag="PLS34_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRP86936.1"
                     /translation="MSFFAHYNYHLAIILAMIGFYTMISRTNMVKKVAGLNIFQVSVF
                     ILFISMGVLDGGTVPILAEGYERYSNPLPHVLILTAIVVGVATTAVALALVVRIREAF
                     GTVEEDQVQAKDDEP"
     gene            5423..6832
                     /locus_tag="PLS34_05395"
     CDS             5423..6832
                     /locus_tag="PLS34_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012156498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HRP86937.1"
                     /translation="MLPAGRLPWLWATAVSWFMLAAAISLAAQVLDVGVLSYEMGGWA
                     PPIGIEYRLDPANALLALLIAALAAVVLPFAGRSIASEVPEEKSPYVYAALLLFLAGQ
                     MGIAVTGDAFNVFVFLEISSLATYTLIALGRNRRALTASFSYLIMGTVGATFFLIGVG
                     LLYAMTGTLNMADLAARIPDVAATRTVHTAYAFIVIGVCLKLALFPLHLWLPNAYTYA
                     PSAVTALMAATATKVAIYVLIRFIYTVFGPTFSFGDMPTVYLLVPLAVVGLLSTSVVA
                     IGQDKIKRMLAYSSVAQIGYMVLGIGMATTAGLTAALLHVFNHALMKGALFMALGCIA
                     IRFGTSQLDAFRGLGRRMPFTMAAFVAGGLSLVGVPLTVGFISKWYLVLAAIELGWWP
                     IVAVIIVGSLLAVIYIWRVVEVAYFQQGESTERCEAPLSLLVPTWILIGANFWFGVDT
                     RVTVGLAERAAQLLAGGGP"
     gene            6832..8301
                     /locus_tag="PLS34_05400"
     CDS             6832..8301
                     /locus_tag="PLS34_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HRP86938.1"
                     /translation="MLSASTALEIAVLVPLIGALLIVLAGRWPNLRETVTLVTAVALF
                     GVVMRILWGLTHEEVVEITLIEMLPGLSIAFHTEPLSILFAAVASGLWVVTSVYSIGY
                     MRGNNEQHQTRFYACFAIALSAAMGVAFSANLLTLFVFYEVLTLSTYPLVAHKGDEAA
                     KRGARTYLGILLATSIGLLLPAIIWTWSVAGTLDFTPGGVLEGRITGGLATVLLLMFV
                     LGTGKAAVMPVHRWLPAAMVAPTPVSALLHAVAVVKAGVFAITKIVIYIFGLDFLVDV
                     PEERLLVYLVGFTIIVASIVALRQNNLKRMLAYSTVSQLSYIVLAALVLAPLSEIGAA
                     VHIVAHAFGKITLFFAAGAIYVASKKTEITQLSGIGRRMPWTMTAFAIGGLSMVGVPP
                     TAGFVSKWYMIAGAFQNDNYFVLAVLIIATALNAAYFLPIIFRAFFDTENVPPKKEHG
                     EAPWPMVAALTTTAALVLLFFLYNGPVVTLEYGILKGPG"
     gene            8298..8570
                     /locus_tag="PLS34_05405"
     CDS             8298..8570
                     /locus_tag="PLS34_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86939.1"
                     /translation="MSERDDKDKQLHWLVRPATIRRLWQVFGVVLGLTVAAGLFVESH
                     GYFGLDGTFGFNAWYGFLACVAMVVVAKLLGFLVKRPESYYRDRDV"
     gene            8563..10248
                     /locus_tag="PLS34_05410"
     CDS             8563..10248
                     /locus_tag="PLS34_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HRP86940.1"
                     /translation="MFEIIIPPALILLIGAFLVAVVQPALRPVIALAAPLATLYAIWQ
                     VPDGILLSATFLGYEVQLIEGGPLRRMFATVFAMMAFAGALFSFKQAKWYELAAAMGY
                     ASGAIGVSFAGDLIVMFVFWELMALFSVVVVWSGGTFGSKRAGIRYIIMHLLGGIILK
                     VGIEGIMVHTGSVEIRPLALDNFDTWMVFTGILINAAAPPVSAWLADAYPESSPTGSV
                     FLSAFTTKTAVLALILLFPGQPLLIWIGLWMVFYGIIYALLENDIRRILSYSIINQVG
                     FMVVAVGIGTELALNGAAAHAFAHIFYKALLFMSAGAVIYQTGKNKCTELGGLFQSMP
                     LTTVCGIIGALSISSFPLTSGFVSKGLTTAAAAEAQLGVVWFLLAAASAGVFLHAGIK
                     FPWFVFFQKDSGLRPKDPPWNMQAAMVFLSVLCIVIGVFPGLLYWMLPTPVEYVPYTA
                     DHVVAMLQLLLFAGLAFFVMLPMMKRTLTISLDLDWFWRVAWVRVFRSVERLANGLRE
                     GLGAAVTGLLKRFSDGLMRHHGAQGVLARAWPTGSMLLWMAVLLAVFMIARYA"
BASE COUNT         1407 a         3439 c         3477 g         1949 t
ORIGIN      
        1 gacgccggtc tcctcggcca gccgctgcag cagccgcagg gtcttcttgc tcacgcggtt
       61 acgcgagtat tcgagcagca gcccctcggc ctcgaccgag aagcgcgtgt agcgctcggg
      121 gtcgcgcctg aacaccgcgc gcaggtggcg tttcgcgatg tcctcgcgat agtgctcctt
      181 cagcgcccgc caggccttga gacccctggg ccagttcacc gccttgctcg tcatgcgccg
      241 cgctccgtgc cgccgggcca acaccccatt gtcaacgctg atcgttacgg taataagaca
      301 ggtaccagtc cacgaagcgg gcgatgccct cctcgaccgg cgtctgcggc cggtagccga
      361 cgtcctcgac cagcgcctcg acgtccgccc aggtatcggg cacgtcgccg ggctgcatcg
      421 gcaggagctc tttctcggcc ttcttgccga gccgctcctc cagcacctcg atgtagcgca
      481 tcagctcgat cgggccctgg ttgccgatgt tgtagagccg gtacggcgca aagctggtgc
      541 ccgggtccgg ggcgttgctg tcccaggcgg ggttcggtgc cgcgaccttg tcgaggcagc
      601 gcaccacccc ctcgacgatg tcgtcgatgt aggtgaagtc gcgccggtgg tggccgtagt
      661 tgaacacctc gatgggctgg ccctcgagga tcgccttggt gaacttgaac aacgccatgt
      721 ccgggcggcc ccaggggccg tacacggtga aaaacctgag ccccgtggtg gggagctggt
      781 acagcgccga gtaggtgtgc gccatcagtt cgttggcttt cttggtggcg gcgtagaagg
      841 acagcggatg atcgacgttc tggtgcaccg agaacggcat cttggtgttc gcgccataga
      901 ccgagctggt ggaggcatag accaggtgct cgacctcggc atggcggcag ccttccagca
      961 ggttggtctg gccgaccagg ttgctctcga cgtaggccat cgggttctcg atggaatagc
     1021 gcacgccggc ctgtgccgcg aggtgcacga cgcggtcgaa tttctcgctc ttgaacagct
     1081 ccatcatgcc gctgcgatcg accaggtcga gcttcctgaa acgccagttg cgcttgtcct
     1141 gcagccggct gagccgggcc cgcttgagcg tcggatcgta gtactcgttg aggttgtcga
     1201 gccccacgac ctcgtcaccg cgctcgagca gtttcttcgc ggtgtggaag ccgatgaagc
     1261 cggcggcgcc ggtgaccagt actttcattg cgtcagtcca ttgcgtgcaa ttggggacat
     1321 tccccaattc ggtagattga attggggaat gtccccatta cagccggccg tcgacggcgt
     1381 cggccggcaa tatgtgcttg atatcgtaga cgaccgaggt ctcggcgccg aaggcgcgca
     1441 ccccggcggc gccgagcgcg cggaactcgc gatgggcaac ggccagcacc acggcgtcgt
     1501 aggcgcccgc ggccggttcg gccaccggcg cgatgccgta ctcggcctgc gcctccgcgg
     1561 cgtcgaccca cgggtcgtgc acgtccacct gcacgccgac gtccgccagc tcgcgcacca
     1621 ggtcgaccac cttggtgttg cggatgtcgg ggcagttctc cttgaaggtc agcccgagca
     1681 ccagcacccg gctgccgcgc acctggatgc cggcgcgcat catcagccgc aggatacggt
     1741 cggccacgta cagcgccatg ttgtcgttga tgcgccgccc ggcgaggatc atctcggggt
     1801 tgtagccgac ttactggctc ttgtgcgtga ggtaataggg atcgacgccg atgcagtggc
     1861 cgcccaccag ccccggccgg aacggcagga aattccactt cgtgccggcc gcctctagca
     1921 cctcgagggt gtcgatgccg agccggttga agatcatcgc cagctcgttg atcagcgcga
     1981 tgttgacgtc gcgctgggta ttctcgatga ccttggcggc ctcggccacc cggatggacg
     2041 aggccttata ggtgcccgcg gtgatcacgc tggcgtacag cgtatcgacg aagtccgcca
     2101 cttccggggt cgagcccgag gtcaccttga ggatgctctc gacgcggtgc tccttgtcgc
     2161 ccgggttgat gcgctcgggg ctgtagccgg cgaagaaatc gacgttgtac ttgagcccgg
     2221 cgccccgctc gatgatgggc acgcacactt cctcggtggc gcccgggtag acggtggact
     2281 cgaagatcac ggtgtcgccg cgcttgacca gttcgccgag ggtgcggctg gcgcgcttca
     2341 ggggcgacag gtccgggatc ttggccgagt cgatcggcgt cggcacggtg acgatgtaga
     2401 cgttgcaggc cgccagcccc gccgggtcgc tggtgaaggc caggtgttcg gccgaggcga
     2461 gctcctccgg cgaggtctcc agcgtggcgt catgcccgga ctccagctcg gcgatacgat
     2521 cggccttcag gtcgaagccg acggtcgggt agcggcgccc gaaggcgacc gccagcggca
     2581 ggccgacgta ccccaggccg atgatgccga tcttcacctg gtccatggcg ggcaatttca
     2641 ctgcggcagg ctcctcggat ggcaatgcgc cattgtacgg ccgcccccgc cggcgcccaa
     2701 gacgagggtc acagaaccgg gcgccggagc ctggattctt ggcgtggatt gcccggccgt
     2761 ctatcattgc gccttcgcga agctcccggc gcgggccgtt tccacggcgc gcacgggtga
     2821 atgctggccg atttccagtc ggccgcgccc acggaaccag gacggccgtt gaacaagcga
     2881 atcctcaatc tgctgctgat gggactgatg ctggcggccg tctggctgct gctgtcgggc
     2941 ctgttcaagc cgctgctggt gggcctgggg atggctgcga cgttgctgac gctgtggctg
     3001 gcgagtcgca tgggcatcat cgacgcagca acgcacccgg tatgggccgt gttccgcttc
     3061 gtgtcgttct ggccctggct gaccgtcgag atcatcaagt cgagcatcga cgtggcgcgc
     3121 cgcgtgctgt cgccctccat gccgatcagc ccgaccgtgt tcgaactgcg ctccacgcag
     3181 aagacggtgg tcggccgggt ggtgctggcc aattcgatca cgctgacgcc cggcacggtc
     3241 acgctcgacg tggacggcga ccgcctgacg gtgcatgcgc tggccagcga gtcggtcgaa
     3301 tacgccctga gcggcgagat ggaccggcgc gtgagccggg ccgaaggtcc gccggccgac
     3361 ggtgcggcgc ccccgggcgg ccgggcggga gacgccgcat gatcgccgcc gccatcctct
     3421 cgatcatggt caccatggcg ctcgccgtga tccgcgcgct gctgggcaag accgtgtacg
     3481 accgcatcct ggcggtaaac gtgttcggca ccaagaccgt gctgctgatc gcggcgctgg
     3541 ccttcctcac cggccggccg gacgtgctcg acatcgcgct ggcctacgcg ctgatcaact
     3601 tcatcggcat gatcgccgtg ctgcgctact tcgagtacgg cggcgccgag cccgccggcc
     3661 gggagtccga ctgatggccc tggcggtgga cctgctgagc tgggcgctga tcctcatcgg
     3721 cggcgccttc ggcatcatcg gcggcatcgg cctgctgcgg ctgcccgatt tcttcagccg
     3781 catccacgca gccagcctca ccgactcgat gtgcgcgccg tgcatcatcg ccggcctgat
     3841 gctgcagtcg ggcttcaccc tggtcacggt caagctcggc ttcctcatcg tgttcctgtt
     3901 tctgaccagc ccgacggcct cgcatgcgct cgccaaggcg gcgctgcgcg gtggcgtcga
     3961 gtccggccag cgccatccgg ggccgcagga ggaacggcgc tgatggacac gctggtcgac
     4021 atcctgctgc tggccttcct ggcgatctgc gccatcgcca tcctgcggct gcgcgacctg
     4081 ttcgccatgg cgatgatgtt cggcctgttc agcctcatct cggccgggct gttcaccacc
     4141 atggacgcgc cggacgtcgc cttcacggag gcggccgtcg gcgcgggcat ctcgaccatc
     4201 ctgatcctcg ccaccctcgc ccagacggcg cgccgcgagc agcgccagtc cggacagccg
     4261 aggctggtgc ccttgctggt ggtctggatc accggcgccg tgctgatcta cggcacggcc
     4321 gacctgcccg ccttcggcga ccccgcggcg ccggcgcaca accacgtggc gccgcactac
     4381 ctgcaggagt caccgaacga gatcggcatt cccaacatcg tcgcggcggt gctcgccagc
     4441 taccgcggct tcgatacgct cggcgaggtc atcgtggtgt tcgccgcggt ggtcggcgtc
     4501 atctccctga tcggcctgcg gcggcgcccg gagggcgtca agccgaatgc cgccgcgctc
     4561 agccagcacg ccgtgctgca ggtcatcgcc aagctggtgg tgccggtgat cctgctgttc
     4621 ggcctgtacg tgcagttcca cggcgagtac agcccgggcg gcggcttcca ggcaggggtg
     4681 ctgttctccg ccggcatcat tctctatgcg ctggtcttcg gcctggacgc cgcgcgcagc
     4741 gtcatcccgc cgaaagtgct catcgccctg cccggcatcg gtgcgctgat tttcggcggg
     4801 gtcggcgtcg tgaccctgct gctgggcggc aactacctcg actacgacca gctcggcgcc
     4861 gacccggtgg cggcgcagca cctcggcatc ctgctggtgg agctcggcgt cggcgtgacc
     4921 gtcgcctcgg tgatgctgct catctatttc accttcgccg agcgcggccg cggcaagcgc
     4981 gccgacgacg gggaggcggc cgaatgagct tcttcgccca ttacaactat cacctcgcca
     5041 tcatcctcgc gatgatcggc ttctacacca tgatctcgcg caccaacatg gtgaagaagg
     5101 tcgccggcct gaacatcttc caggtgtcgg tgttcatcct gttcatcagc atgggcgtgc
     5161 tggacggcgg caccgtgccg atcctcgcgg agggctacga gcgctactcc aacccgctgc
     5221 cgcacgtgct gatcctgacc gccatcgtgg tcggcgtggc gaccacggcc gtcgcgctgg
     5281 cgctggtggt gcgcatccgc gaggccttcg gcacggtcga ggaagaccag gtccaggcca
     5341 aggacgacga gccctgaacg ccgcgccgca cctgctcgcg ctgatcgtcg tcgcgcccct
     5401 gctgggcgcg gcggtctcga ctttgctgcc cgccggccgc ctgccctggc tgtgggccac
     5461 ggcggtgagc tggttcatgc tggcggcggc gatttcgctg gcggcgcagg tgctggacgt
     5521 cggcgtactg agctatgaaa tgggcggctg ggcgccgccg atcggcatcg agtaccggct
     5581 ggatccggcc aacgcgctgc tggcgctgtt gattgccgcg ctcgcggccg tggtgctgcc
     5641 cttcgccggc cgcagcatcg cgtccgaggt gccggaggag aagtcgccct acgtctacgc
     5701 ggcgctgctg ctgttcctgg ccggccagat gggcatcgcg gtcaccggcg acgccttcaa
     5761 cgtgttcgtg tttctcgaga tctcctcgct ggcgacctac acgctgatcg ccctgggccg
     5821 caaccgccgc gccctgaccg cgtcgttctc ctatctcatc atgggcacgg tcggcgcgac
     5881 ctttttcctc atcggcgtcg gcctgctgta cgccatgacc ggcacgctga acatggcgga
     5941 tctcgccgcg cgcatccccg atgtggcggc gacgcgcacc gtgcacacgg cctacgcctt
     6001 catcgtgatc ggcgtctgcc tgaagctggc gctgttcccg ctgcacctgt ggttgccgaa
     6061 cgcttatacc tacgccccca gcgccgtgac cgcgctgatg gcggccaccg ccaccaaggt
     6121 ggcgatctac gtactgattc gattcatcta cacggtgttc gggccgacct tctccttcgg
     6181 cgacatgccc accgtctacc tgctggtgcc cttggcggtg gtcggcctct tgagcacctc
     6241 ggtggtggcg atcggccagg acaagatcaa gcgcatgctg gcctattcct cggtggcgca
     6301 gatcggctac atggtgctgg gcatcggcat ggcgaccacg gcggggttga ccgcggcgct
     6361 gctgcacgtg ttcaaccatg cgctgatgaa gggcgcgctg ttcatggcgc tcggctgcat
     6421 cgccatccgc ttcggcacct cgcagctgga cgccttccgc ggcctcggac ggcgcatgcc
     6481 cttcaccatg gcggccttcg tggccggcgg cctcagcctg gtcggcgtgc cgctgacggt
     6541 cggcttcatc agcaagtggt acctggtgct ggcggccatc gagctcggct ggtggccgat
     6601 cgtcgccgtc atcatcgtcg gctcgctgct ggcggtcatc tacatctggc gggtagtcga
     6661 ggtggcctac ttccagcagg gcgagagcac tgaacgctgc gaggcgccgc tgtcgctgct
     6721 ggtgccgacc tggatcctga tcggcgcgaa cttctggttc ggcgtcgata cccgcgtcac
     6781 ggtcggcctg gccgagcgtg ccgcgcagct gctggcggga ggcgggccct gatgctcagc
     6841 gcgtccaccg ccctggagat cgcggtgctc gtgccgctca tcggcgcgct gctgatcgtg
     6901 ctggccggcc gctggccgaa cctgcgcgag acggtcacgc tggtgacggc ggtggcgctg
     6961 ttcggcgtgg tgatgcgcat cctgtggggc ctgactcacg aagaggtcgt cgagatcacg
     7021 ctcatcgaga tgctgcccgg cctcagcatc gctttccaca ccgagccctt gtcgatcctg
     7081 ttcgccgccg tggccagcgg cctgtgggtg gtgacctcgg tctactccat cggctacatg
     7141 cgcggcaaca acgagcagca ccagacgcgc ttctacgcct gtttcgccat cgcgctgtcc
     7201 gcggcaatgg gcgtggcgtt ctcggccaac ctgctcacgc tgttcgtgtt ctacgaggtg
     7261 ctgacgctgt ccacctaccc gctggtggcg cacaagggcg acgaggcggc gaagcgcggc
     7321 gcgcgcacct atctcggtat cctgctcgcc acctccatcg ggctgctgct gccggcaatc
     7381 atctggacct ggtcggtggc cggcacgctg gacttcacgc ccggcggcgt actggagggg
     7441 cgcatcaccg gcggcctggc cacggtgctg ctgctgatgt tcgtgctcgg caccggcaag
     7501 gcggcggtga tgcccgtgca tcgctggctg ccggcggcca tggtggcgcc gacgccggtc
     7561 tcggcgctgc tgcatgccgt ggcggtggtc aaggccggcg tgttcgcgat caccaagatc
     7621 gtcatctaca tcttcgggct cgacttcctg gtggacgtgc cggaagagcg cctgctggtg
     7681 tacctggtcg gcttcaccat catcgtcgcc tcgatcgtcg cgctgcggca gaacaacctc
     7741 aagcgcatgc tggcctactc gaccgtcagc cagctgtcct acatcgtgct ggcggcgctg
     7801 gtgctcgcgc cgctgtcgga gatcggcgcg gcggtgcaca tcgttgcgca cgccttcggc
     7861 aagatcacgc tgttcttcgc cgccggcgcg atctacgtgg cctcaaagaa gaccgagatc
     7921 acgcagctgt ccggtatcgg gcggcgcatg ccctggacca tgacggcctt cgccatcggc
     7981 gggctgtcga tggtgggcgt gccgccgacc gccggcttcg tgtcgaagtg gtacatgatc
     8041 gccggcgcgt tccagaacga caattacttc gtgctcgcgg tgctgatcat cgccactgcc
     8101 ttgaacgcgg cgtatttcct gccgatcatt ttccgggcct tcttcgacac cgagaacgtg
     8161 ccgccgaaga aggagcatgg cgaggcgccc tggccgatgg ttgcggcgct gaccacgacc
     8221 gcggcgctgg tgctgttgtt cttcctgtac aacggcccgg tggtgacgct ggaatacggc
     8281 atcttgaagg gaccgggatg agcgagcgcg acgataagga caagcagctg cactggctgg
     8341 tccgcccggc gaccatccgc cggctgtggc aggtcttcgg cgtggtgctg ggcctgaccg
     8401 tggcggcggg cctgttcgtg gaatcgcacg gctacttcgg cctggacggc accttcggct
     8461 tcaacgcctg gtacggtttt ctcgcctgcg tcgcgatggt ggtcgtggcg aagctgctcg
     8521 gcttcctggt gaagcggccc gagagctact acagggaccg cgatgtttga gatcatcatt
     8581 ccgccggcat tgatcctgct gatcggcgcc ttcctggtcg cggtggtgca gccggcgctg
     8641 cgcccggtga ttgcgctggc ggcgccgctc gcgacgctgt acgccatctg gcaggtgccg
     8701 gacggcatcc tgctgagcgc gaccttcctc ggctacgagg tgcagctcat cgagggcggg
     8761 ccgctgcggc gcatgttcgc cacggtgttc gcgatgatgg ccttcgccgg cgcgctgttc
     8821 tccttcaagc aggccaagtg gtacgagctg gcggcggcga tgggctatgc ctcgggcgcg
     8881 atcggcgtca gcttcgccgg cgacctgatc gtgatgttcg tgttctggga gctgatggcc
     8941 ctgttctcgg tggtggtggt gtggtcgggc gggaccttcg gttcgaagcg cgccggcatc
     9001 cgctacatca tcatgcacct gctcggcggc atcatcctca aggtcggcat cgaaggcatc
     9061 atggtgcaca ccggctcggt ggaaatccgg ccgctggcgc tggacaactt cgacacctgg
     9121 atggtgttca cgggcatcct catcaacgcc gcggcgccgc cggtcagcgc ctggctggcg
     9181 gacgcctacc cggagtcctc gcccaccggc tcggtgttcc tgtcggcctt caccaccaag
     9241 acggcggtgc tggcgctgat cctgctgttc cccggccagc cgctgctgat ctggatcggc
     9301 ttgtggatgg tgttctacgg catcatttac gcgctgctcg agaacgacat ccggcgaata
     9361 ctttcgtact cgatcatcaa ccaggtcggc ttcatggtgg tggccgtggg catcggcacc
     9421 gagctggcgc tgaacggcgc cgcggcgcat gccttcgcgc acatcttcta caaggcgctg
     9481 ctgttcatga gcgccggcgc ggtgatctac cagaccggca agaacaagtg caccgagctc
     9541 ggcgggctgt tccagagcat gccgctgacg acggtgtgcg gcatcatcgg cgcgctgtcg
     9601 atctcgtcat tcccgctgac ctcgggcttc gtctccaagg gattgacgac cgcggcggcc
     9661 gcggaggcgc agctcggcgt ggtgtggttc ctgctcgccg cggcctcggc cggcgtgttc
     9721 ctgcatgccg gcatcaagtt cccgtggttc gtgttcttcc agaaggactc ggggctgcgg
     9781 cccaaggacc cgccgtggaa catgcaggcg gcgatggtgt tcttgtccgt gctgtgcatc
     9841 gtgatcggcg tgttcccggg gctgctgtac tggatgctgc cgacgccggt ggagtacgtg
     9901 ccgtacacgg cggatcatgt cgtcgccatg ctgcagctgc tgctgttcgc cgggctggcg
     9961 ttcttcgtga tgctgccgat gatgaagcgc acgctgacca tcagcctcga cctggactgg
    10021 ttctggcgcg tggcgtgggt gcgggtgttc cgctcggtgg agcggctggc caacggcctc
    10081 cgggaggggc tcggggccgc cgtgaccgga ctgctgaagc gcttctccga cggcctgatg
    10141 cgccaccacg gcgcccaggg cgtgctggcg cgcgcctggc cgaccggcag catgctgctg
    10201 tggatggccg tgctgctcgc cgtcttcatg atcgcccgct acgcctgagc gggtcggacc
    10261 atcccaaggt ac
//
LOCUS       DAOMPG010000070        10228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2829_length_10228_cov_4.518726, whole genome shotgun sequence.
ACCESSION   DAOMPG010000070 DAOMPG010000000
VERSION     DAOMPG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_10228_cov_4.518726"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLS34_05415"
     CDS             complement(<1..484)
                     /locus_tag="PLS34_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP86941.1"
                     /translation="MKPLYRTCPESGLVFHKSAENLIKANAVAAVVFLMVGGLLGLFV
                     ALTRWPAVQILDAQMFYQVLTGHGIVTLLVWIIFFEIALLYFCAAVLLRSRLATPRIG
                     WLAFALMVIGAAVTAVSILQGGSSVMMTSYVPMPAAPHFYLGLILFAVGALIGCFIFL
                     G"
     gene            complement(513..1055)
                     /locus_tag="PLS34_05420"
     CDS             complement(513..1055)
                     /locus_tag="PLS34_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HRP86942.1"
                     /translation="MSAIEPPAQRIWWKQPLDRLEGTWIVIALIWCLIMFFMMPYWHV
                     FGQQNYKGEAYRVLPQAYVEKVGEMVDAYTVRHETDRQIPVVAPPAGSDIYLLARLWE
                     WYPVLELKKGESYRLHISSADWQHGLSIQPININVQVLPGYDMVMTITPTASGEYALV
                     CNEYCGALHHAMLGKLYVVD"
     gene            complement(1071..1250)
                     /locus_tag="PLS34_05425"
     CDS             complement(1071..1250)
                     /locus_tag="PLS34_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86943.1"
                     /translation="MNDEHDARHDDDAPEGEKVPVMQQVLDNPFLLLFLGITVPTVFY
                     IIWGVMEIVTIPLAP"
     gene            1384..2043
                     /locus_tag="PLS34_05430"
     CDS             1384..2043
                     /locus_tag="PLS34_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP86944.1"
                     /translation="MTRCNAKIRGVAPLAAVTLALAAAAGCGRDAAPGPAWDFAAVDA
                     GGNVVVEGASGPHECVLDRRTGLLWEVKQAEAGLRHRDDLYTWYSSDIAEHNGEPGLM
                     NGGRCALERCDTEAFVAAVNATGLCGRQDWRLPSRDEALTLLDTRRIGDGPTLDPQYF
                     PHAVSAECWTATTFRLYPQGAWAIEPIYAQDRVDWKTVPKCVRLVSGSKDAVRPKRRG
                     R"
     gene            2040..3113
                     /locus_tag="PLS34_05435"
     CDS             2040..3113
                     /locus_tag="PLS34_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRP86945.1"
                     /translation="MSAHVSADERRIAAWLLACCVVLFALIVLGGVTRLTGSGLSITE
                     WRPVTGVLPPFTEQAWQQEFDLYRASPEYQKVNRGMSMDQFKVIYGYEYAHRLLARAL
                     GLVFAVPLLWFWVRGRIPPRLRWPLVGLLGLGMLQGFMGWFMVASGLVDMPRVSQYRL
                     AAHLSLALIIFAAMFWLAIRLLRPTVEGTVPGNVRRGMQALLWLLAVTIVSGAFVAGL
                     RAGMIYNTFPMMGEQWVPGGLLAMDPAWRNWFENPVTAQFTHRVLALTTLGLVLVLWG
                     WSLRLRVARAQQWAFHALALMAVGQVVLGISTLLLFVPVGLGAAHQGGAVLLLGATLF
                     ALHEVGGARQAVGARVPAEARLA"
     gene            complement(3110..3775)
                     /locus_tag="PLS34_05440"
     CDS             complement(3110..3775)
                     /locus_tag="PLS34_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP86946.1"
                     /translation="MTATAAGRRLPAGPLLLGVLGLVILGAVLLARQGPLESPGWANA
                     PAELRAILWPEPLPIPEFELLDQHEQPFGPAGFEGRWTFLFFGYLQCPDVCPTAMHLL
                     REYRRTLSASEPGLAHQVVFVSVDPAYDRPEQIAAYMDFFDPEFIGLSGPADALAALA
                     GPLAIKYYEHVDDNGRRAIDHTSSIMIVDPAGRVLGAFPPPQQRPDRMLALFRELRRH
                     FSL"
     gene            complement(3783..4130)
                     /locus_tag="PLS34_05445"
     CDS             complement(3783..4130)
                     /locus_tag="PLS34_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP86947.1"
                     /translation="MRKLSLMAAMAALALINAPAHAGDADAGKAKFQQICAVCHGPAG
                     AGDGPASAGLNPKPRKFNDAEWQASVDDAYLKKIITEGGPAVGKSPMMTAQRGLKDEE
                     VANIIAYVRSLAK"
     gene            4339..4653
                     /locus_tag="PLS34_05450"
     CDS             4339..4653
                     /locus_tag="PLS34_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRP86948.1"
                     /translation="MKNRTAPSKLEEARAEQALYRAILALQDADEAGRFFRDLCTPAE
                     LQALVDRWRVVGLLRAGLPYRQINEQTGISVTTIGRVARFLADGNRGYLVVAERLDGE
                     NP"
     gene            4650..5528
                     /gene="hisG"
                     /locus_tag="PLS34_05455"
     CDS             4650..5528
                     /gene="hisG"
                     /locus_tag="PLS34_05455"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRP86949.1"
                     /translation="MTIQAEQRIKVAVQKSGRLTENSLHLMETCGLIYSRGRDQLICF
                     GENMPVDVLLVRDDDIPELVAEGICDLGIVGMNVFREKVADGMNVRPELELDFGRCRL
                     SIAVPRGFDYASARSLEGTRIATSYPRILKAWLDEQGVSAQVVTLSGAVEIAPRLGRA
                     DVICDLVSTGSTLLANDLVEAEVVFESKAALISRNGGPAEGWPARLVSRMRGVLSVRE
                     SKYIMLHAPREALAEIRRLLPGSESPTVMPLEGRDDKVAVHAVCRENVFWETLEGLKA
                     AGASSMLVLPVEKMLA"
     gene            5539..5706
                     /locus_tag="PLS34_05460"
     CDS             5539..5706
                     /locus_tag="PLS34_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86950.1"
                     /translation="MLRRFTWAALDEAGRAAALARPEAAQREALAAQVAAIIAETRRE
                     RRVQRTPRLRR"
     gene            5711..7000
                     /gene="hisD"
                     /locus_tag="PLS34_05465"
     CDS             5711..7000
                     /gene="hisD"
                     /locus_tag="PLS34_05465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRP86951.1"
                     /translation="MTALRRVTLFHPDDRGSVEARMQRRRDGDAALRRFAQRFDGVTL
                     ASLRVPAAEFDAAEASLTEVQRAALRTAIDNVRAFHAAQLPGPVEVETMPGVVCRRIT
                     RPIRAVGLYVPAGSAPLPSTVIMLAVPAALAGCPRRVLCTPPQRDGRADPAVLTAARY
                     CGIDEVYLAGGAQAVAAMAYGTESIPAVDKIFGPGNAWVTEAKLQVSRDPDGAALDLP
                     AGPSEVLVIADQRADPGFVAADLLSQAEHGPDSQVLLVTTSTALADAVDGAIREQLAE
                     LPRNDVVREALAHGRAFIAHDLHEAFAISNRYAPEHLIVQVPEAEAWLDCIEVAGSVF
                     LGDWTPESLGDYCSGTNHVLPTYGYARAWSGLSVQSFQLSVSVQHATPEGFRVIGPTA
                     RTLAELERLDAHALAVLLRLEALARHSASDRSAKARG"
     gene            6997..8088
                     /gene="hisC"
                     /locus_tag="PLS34_05470"
     CDS             6997..8088
                     /gene="hisC"
                     /locus_tag="PLS34_05470"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRP86952.1"
                     /translation="MSGVAHLLRPELRDFSAYHAASPRRGVIRLHANESSWRADWDDT
                     DDGLNRYPDPRPAALVNALGGLYGVAPGAILVTRGSDDAIDLLVRAFCRAGRDAVLVC
                     PPTFGMYAVAARLQGADVMEVPLQRNFEPDTAAIAAAARGREGVKLVFLCSPNNPTGN
                     TIAPTAVDTLCRALDGHSMVVVDEAYVEFAGDEGFAGLLEAHANLVLLRTLSKAWGLA
                     GARVGCLLGDPELVAILRALAPPYPLPSLATEAALRRLQPAELAAARRRTAGVVRRRE
                     ALAEALRTLSAVQRVWPSEGNFLLARFNDAAAALAACESAGVLVRDFSRQPRLEGCLR
                     ITVGTAVENRRLLAALGRVAARSARRKNG"
     gene            8081..9163
                     /gene="hisB"
                     /locus_tag="PLS34_05475"
     CDS             8081..9163
                     /gene="hisB"
                     /locus_tag="PLS34_05475"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRP86953.1"
                     /translation="MDRALLLFIDRDGTLVEEPADEQVDALEKVRLLPGVIPALLQLV
                     DAGYRLVLVSNQDGRGTESFPEQDFQRTHDFIMELFASQGIRFEAEFICPHFPQDGCA
                     CRKPATGLLTRFIAQHPPDLDRSFVLGDRDTDLAFAENLGLVGLRVGDGTGDSLTWPE
                     AARRILGGLRRAIVTRGTAETRIRVEVDLDDPHGPVRAATGIGYYDHMLEQVAKHGGF
                     SMELECSGDLHVDEHHTVEDVALALGEALRQALGTRRGIRRYGFVLPMDETRAELSID
                     LGGRPYLVFHGEFHRERVGELPTELVPHFFRSLADSLRASIHVRLDGENDHHQVEGAF
                     KALGRALREAFRREGGGIPSTKGMLA"
     gene            9160..9759
                     /gene="hisH"
                     /locus_tag="PLS34_05480"
     CDS             9160..9759
                     /gene="hisH"
                     /locus_tag="PLS34_05480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRP86954.1"
                     /translation="MTGGPVIVDSGGANISSLRFALGRLGCSAPLTADAGRIRGASHV
                     FLPGVGAADEAMTRLERSGLADLVRGLRQPVLGICLGMQLLFEASDEDGGRACLGLIP
                     GCVRRIPGGPDRPVPHMGWNLTSLAPDGALFAGLPGAAHFYYVHSYAAPPGEHTVAVA
                     DYGGEFTAVVSCGNFHGAQFHPERSGPAGARVLANFLAL"
     gene            9781..>10228
                     /locus_tag="PLS34_05485"
     CDS             9781..>10228
                     /locus_tag="PLS34_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRP86955.1"
                     /translation="MDLKPIPAIDLKAGRVVRLYQGRFDQVSEYQADPESLAARYLSL
                     GVDWVHVVDLDGARDGAGGNREVIARMAGLARGRIQVGGGIRSRDDVAAMLATGAGRA
                     VIGSTAVERPAEVCGWFGEFGAERLVLAFDVQLDESGVRRCRTRGWT"
BASE COUNT         1501 a         3425 c         3652 g         1650 t
ORIGIN      
        1 tgccgagaaa gatgaagcag ccgatcagcg cgcccaccgc gaacaggatc agccccaggt
       61 agaagtgcgg cgcggcgggc atcggcacgt aagaggtcat catcacgctg gagccgccct
      121 gcaggatcga cacggccgtg acggcggcgc cgatgaccat cagcgcgaag gccagccagc
      181 cgatgcgcgg cgtggccagc cgcgagcgca gcagcaccgc ggcgcagaaa tacagcagcg
      241 cgatctcgaa gaagatgatc cacaccagca gcgtgacgat gccgtggccc gtcaacacct
      301 ggtagaacat ctgcgcgtcg aggatctgca ccgccggcca gcgcgtcagc gcgacgaaca
      361 ggccgagcag gccgcctacc atgaggaaga cgaccgccgc gacggcgttc gccttgatca
      421 ggttctcagc ggatttgtgg aataccagac ccgattccgg gcaggtacgg tagagtggct
      481 tcattattct ctcctccccg atttcaggtc tgtcagtcga ccacgtagag cttgccgagc
      541 atggcgtggt gcaacgcccc gcagtactcg ttgcagacca gcgcatattc cccggacgcc
      601 gtcggcgtga tggtcatcac catgtcgtag ccgggcagca cctgcacatt gatgttgatc
      661 ggctggatcg acagcccgtg ctgccagtcg gccgatgaga tgtgcagccg gtaactctca
      721 cccttcttca gttcgagtac cgggtaccac tcccagagcc gcgccagcag gtagatgtcg
      781 ctgccggcgg gcggcgccac caccggaatc tggcgatccg tctcgtggcg cacggtgtag
      841 gcgtcgacca tttcgccgac tttctcgacg taggcctgcg gcagcacgcg gtaggcctcg
      901 cccttgtagt tctgctgccc gaagacgtgc cagtagggca tcatgaaaaa catgatcagg
      961 caccagatca gggcgatgac gatccaggtg ccctccagcc ggtccagcgg ttgtttccac
     1021 cagattcgct gggcaggggg ttcgatggcg ctcatgctca ggtcctccgg tcagggcgcg
     1081 agggggatgg tgacgatctc catcactccc cagatgatgt agaacacggt cggtaccgtg
     1141 atccccagga acagcagcag gaaggggttg tcgagcacct gttgcatcac gggtactttt
     1201 tcgccctccg gcgcatcgtc gtcgtgccgc gcatcgtgtt cgtcgttcat gtgttcccct
     1261 cctcccttgg gccctatagt ggtatataat gtacatcaat tgaatgatgc gcaagctagc
     1321 attcatgtgg ttcagattta ttgatgtaga tcaacttcgg ctttctcggc cctggagggc
     1381 gacatgactc gctgcaatgc aaagatcagg ggcgtggcac cgctcgccgc cgtcacgctg
     1441 gcgctggcgg cggcagcagg ctgcgggcgc gacgcggcgc cgggcccggc atgggatttc
     1501 gccgcggtcg acgccggcgg gaacgtggtg gtggagggtg cgtcgggccc gcacgagtgt
     1561 gtgctcgacc ggcgcaccgg gctgctgtgg gaggtcaagc aggccgaggc gggcttgcgc
     1621 caccgggacg atctgtacac gtggtacagt tcggatattg cagagcacaa cggggaaccc
     1681 gggctgatga acggcgggcg ttgcgccctg gagcgctgcg ataccgaggc cttcgtggcc
     1741 gccgtcaatg ccaccggcct gtgcggtcgg caggactggc ggctgccgag ccgcgacgag
     1801 gcgctgaccc tgctggatac ccggcggatt ggtgacgggc cgacgctcga tccgcaatac
     1861 ttcccgcacg cggtcagcgc ggagtgctgg acggccacga cctttcgcct ctatccgcag
     1921 ggcgcatggg ccatcgagcc gatctatgcg caggatcgcg tcgactggaa gaccgtcccg
     1981 aagtgcgtgc gcctggtcag cggctccaag gatgccgtcc gaccgaagag gagagggaga
     2041 tgagcgctca cgtatccgcc gatgaacgcc ggatcgcggc ctggctgctg gcctgctgcg
     2101 tggtgctgtt cgccctgatc gtgctgggcg gcgtcacccg cctgaccggc tccgggctgt
     2161 cgatcaccga gtggcgcccc gtcacgggcg tgctgccgcc attcaccgag caggcctggc
     2221 aacaggaatt cgacctctac cgtgccagcc ccgaatacca gaaggtgaac cggggcatgt
     2281 ccatggacca gttcaaggtg atctacggct atgagtacgc gcatcgcctg ctcgcgcgcg
     2341 cgctcgggct ggtgttcgcg gtgccgctgc tgtggttctg ggtgcgcggc cggattccgc
     2401 cgcgactgcg ctggccgctg gtcgggctgc tgggcctggg catgctgcag ggattcatgg
     2461 gctggttcat ggtggccagc ggcctggtgg acatgccgcg cgtgagccag taccggctcg
     2521 ctgcgcacct cagccttgca ctgatcatct tcgccgcgat gttctggctc gccatccggc
     2581 tgctgcggcc gacggtggaa ggcaccgttc cgggcaatgt tcgccgcggc atgcaggcct
     2641 tgctgtggct gctggccgtc accatcgtgt cgggcgcctt cgtcgccggt ctgcgggccg
     2701 gcatgatcta caacactttc ccgatgatgg gtgagcaatg ggtgccgggc ggtctgctgg
     2761 cgatggaccc cgcctggcgc aactggttcg agaacccggt gaccgcgcag ttcacgcatc
     2821 gcgtcctggc cctgaccacg ctggggctcg tgctggtgct gtggggctgg tcgctacggc
     2881 tgcgcgtcgc acgcgcccag caatgggctt tccacgccct cgcgctgatg gccgtcgggc
     2941 aggtcgtgct gggcatcagc acgctgctgc tgttcgtgcc ggtcggcctg ggagccgcgc
     3001 accagggcgg tgcggtgctg ctgctgggcg ctacgctgtt cgccctgcat gaggtgggcg
     3061 gggcacggca ggcagtcggc gcgcgcgtgc cggcggaggc tcggctggcc tagagcgaga
     3121 agtgccgccg cagctcgcgg aacaaagcga gcatgcggtc gggccgctgc tgcggcggcg
     3181 ggaatgcccc cagcacgcgg ccggccgggt ccacgatcat gatcgagctg gtgtgatcga
     3241 tcgcacgccg gccattgtcg tcgacatgct cgtaatactt gatcgccagc ggcccggcga
     3301 gcgccgcgag cgcgtccgcc gggccgctca ggccgatgaa ctcgggatcg aagaagtcca
     3361 tgtaggccgc gatctgctcg ggccggtcat acgccggatc gacggacacg aaaacgacct
     3421 ggtgggccag gccgggctcg ctcgcgctga gcgtgcggcg gtactcccgc agcaggtgca
     3481 tggccgtcgg gcagacgtcc ggacactgca ggtagccgaa gaacaggaag gtccagcggc
     3541 cctcgaaacc cgccggcccg aacggctgtt cgtgctggtc gagcaactcg aattccggga
     3601 tcggcagggg ttccggccag agaatggccc gcaattcggc gggtgcgttg gcccagcctg
     3661 gcgactcgag cggaccctgg cgggccagca gtacagcgcc caggatcacc agcccgagga
     3721 cgccgaggag caacgggccg gccggcaagc gccggcccgc tgcggtagca gtcacgccgg
     3781 cgttacttcg cgaggctgcg gacgtaggcg atgatgttgg caacctcttc gtccttgagg
     3841 ccgcgctgcg cggtcatcat cggcgacttg ccgacggccg ggccgccctc ggtgatgatc
     3901 ttcttcaggt aagcatcgtc gacgcttgcc tgccactccg cgtcattgaa cttgcgcggc
     3961 ttcgggttga gtcctgccga ggcggggccg tcgccggcgc ctgccgggcc gtggcagacc
     4021 gcacagatct gctggaactt ggccttgccg gcgtcggcgt caccggcgtg cgccggcgcg
     4081 ttgatcaggg cgagggccgc catggcggcc atcagggaga gtttacgcat gcctgggctc
     4141 ctggattcaa ggtctgcctg ggaagcaggt ggaagaatag atactaaaaa tatacatctt
     4201 tggccaatag tcaagcccga gagcgggggg gcagggtgga gtgagcggga tttcggcgta
     4261 taccccttgc ccgtttcatt gtattagcgt attaatacgc taattcaact ttgcggacac
     4321 ccaggctcgc ctgcaattat gaagaatcgc actgcaccat ccaagctcga ggaggcgcgc
     4381 gccgagcagg cgctgtaccg cgcgatcctc gccttgcagg acgccgacga ggccgggcgc
     4441 ttcttccggg acctctgtac ccccgcggaa ttgcaggccc tggtggaccg ctggcgggtc
     4501 gtgggcctgc tgcgcgccgg cctgccatac cgccagatca acgaacagac gggcatcagc
     4561 gtgacgacca tcgggcgggt cgcccggttc ctggccgacg gcaaccgggg ctatctcgtg
     4621 gtcgccgaac gactcgatgg agagaaccca tgacgatcca ggcagagcag cggatcaagg
     4681 tcgctgtcca gaaatcaggc cggctgaccg agaactccct gcacctgatg gagacctgcg
     4741 ggctcatcta cagccgcggc cgcgaccagc tgatctgttt cggcgagaac atgccggtgg
     4801 acgtgctgct ggtgcgcgac gacgacattc ccgagctggt cgccgagggc atttgcgatc
     4861 tcggcattgt cggcatgaac gtattccgcg agaaagtcgc cgacggcatg aacgtccggc
     4921 ccgagctgga gctggacttc ggccgctgcc ggctgtcgat cgccgtgcca cgcggtttcg
     4981 actacgcgtc ggcgcgcagc ttggagggca cgcgtattgc caccagctat ccgcgcatcc
     5041 tcaaggcctg gctcgacgag cagggcgtga gcgcccaggt cgtcacgctg tcgggcgccg
     5101 tcgagatcgc gccgcgcctg ggtcgcgcgg acgtgatctg cgacctggtg tcgacgggct
     5161 cgacgctgct ggccaacgac ctcgtcgagg ccgaagtcgt gttcgagagc aaggcggcgc
     5221 tgatctcgcg caacggcggg ccggcggagg gctggccggc acgcctggtc agccgcatgc
     5281 gcggcgtgct cagcgtgcgc gaaagcaagt acatcatgct gcatgcgccg cgcgaggcgc
     5341 tggcggagat ccgtcgcctg ctgcccggca gtgaatcgcc caccgtgatg ccgctcgaag
     5401 gacgggacga caaggtggcg gtgcacgcgg tgtgccgcga gaacgtgttc tgggaaaccc
     5461 tggaagggct gaaggccgcc ggcgcgagct cgatgctggt cctgcccgtg gagaagatgc
     5521 ttgcctgacc gcaatccaat gctgcgtcgc ttcacctggg cggcgctgga cgaagcaggc
     5581 cgcgcggccg cgcttgcccg tccggaggcc gcgcagcgcg aggcgctggc cgcgcaggtg
     5641 gccgccatca tcgccgagac ccgccgcgaa cgacgcgttc agcgaactcc tcggctacgg
     5701 cggtaagtcg ctgacggcgc tgcggcgcgt caccctgttc cacccggacg accgaggcag
     5761 cgtggaggcg cggatgcagc gccgccgcga cggcgacgcg gccctgcgcc gtttcgcgca
     5821 gcgcttcgac ggcgtgacgc tggcgtcgct gcgcgtcccg gcggccgagt tcgacgccgc
     5881 cgaggcgtcg ctcacggagg tgcagcgggc cgccctgcgt accgcaatcg acaacgtgcg
     5941 ggccttccac gccgcgcagc tgcccgggcc ggtcgaggtg gagaccatgc ccggcgtggt
     6001 gtgccgccgg atcacccggc cgattcgcgc ggtgggcctc tacgtgccgg cgggctccgc
     6061 gccgctgccg tccacggtga tcatgctggc cgtgccggcg gcgctggccg gctgtccgcg
     6121 ccgcgtcctg tgcacgccgc cgcagcggga cggccgcgcg gacccggccg tgctgaccgc
     6181 cgcgcgctac tgcggcatcg acgaggtgta ccttgccggc ggcgcacagg cggtggcggc
     6241 aatggcgtac gggacggagt cgatcccggc cgtggacaag atcttcgggc ccggcaacgc
     6301 ctgggtgacc gaggcgaagc tgcaggtgtc gcgcgacccg gacggcgctg cactggacct
     6361 gcccgccggg ccctcggagg tgctggtgat cgccgaccag cgcgccgacc cgggcttcgt
     6421 ggcggccgac ctgctctcac aggcggagca tggcccggat tcccaggtgc tgctggtcac
     6481 cacctcgacg gcgctcgcgg acgcggttga cggcgccatc cgcgagcaac tcgccgagct
     6541 gccgcgcaac gacgtggtgc gcgaagcgct ggcgcacggt cgtgcgttca tcgcgcacga
     6601 cctgcacgag gccttcgcga tcagcaaccg ctacgcaccg gagcatctca tcgtgcaggt
     6661 gccggaggcg gaggcctggc tggactgcat cgaggtcgcc ggctcggtgt tcctcggcga
     6721 ctggacgccc gaatccctcg gcgactactg cagcggcacc aaccacgtgc tgccgactta
     6781 cggctatgcg cgcgcctgga gcgggctgtc ggtgcaaagc ttccagctga gcgtttcggt
     6841 gcagcacgcg acccccgagg gcttccgggt catcgggccc acggcgcgca ccctggccga
     6901 gctggagcga ctcgatgcgc atgccctcgc cgtgctgctg cgcctcgaag ccctggcccg
     6961 gcactcagcg tcggatcgct ccgccaaggc acgcggatga gcggcgtcgc ccacctgttg
     7021 cggcccgagc tgcgcgattt ctccgcctac catgcggcct cgccgcggcg cggcgtgata
     7081 cgcctgcacg ccaacgagtc ctcgtggcgc gccgactggg acgacaccga tgacggcctg
     7141 aaccgctatc ccgatccgcg ccctgccgca ctcgtcaacg cgctgggcgg gctctacggc
     7201 gtggcgccgg gcgcgatcct ggtgacgcgc ggcagcgacg acgcgatcga cctgctggtg
     7261 cgcgcattct gtcgcgccgg ccgcgacgcg gtgctggtct gcccgccgac cttcggcatg
     7321 tacgcggtgg ctgcgcggct gcagggtgcc gatgtcatgg aggtgccgct gcagcgcaac
     7381 ttcgagccgg acacggcggc gatcgcggcg gcggcgcgcg gccgcgaggg cgtcaagctg
     7441 gtgttcctgt gctcgcccaa caaccccacc ggcaacacca tcgcgccgac agccgtggac
     7501 acgctgtgcc gcgcgctgga cggccacagc atggtggtgg tcgatgaggc ctacgtggag
     7561 tttgccggcg acgagggctt cgccgggctg ctcgaggcac atgccaatct cgtgctgctg
     7621 cgcaccctgt cgaaggcctg gggcctggcc ggggcgcgtg tcgggtgcct gctgggcgat
     7681 ccggagctgg tcgccatcct gcgcgcgctg gcgccgccct atccgctgcc gtcgctggcc
     7741 accgaggccg ccttgcgacg cctgcagccc gccgagctcg ccgcggcccg gcgccgcacg
     7801 gcgggggtgg tgcgccgccg cgaggcgctg gccgaggcgc tgcgcacctt gtcggcggtg
     7861 caacgcgtgt ggccgagcga gggcaatttc ctgctggcgc gcttcaatga cgctgcagct
     7921 gcgctggctg cctgcgagtc tgcgggcgtg ctggtgcgtg atttcagccg ccagccgcgg
     7981 ctcgagggct gcctgcgcat caccgtgggc acggctgtcg agaaccgccg cttgctcgcg
     8041 gcactgggcc gcgtggccgc gagatctgcg aggaggaaga atggataggg cgctgctgct
     8101 gttcatcgat cgcgacggca cgctggtcga agagccggcc gacgagcagg tggatgcgct
     8161 ggagaaagtg cgcctgttgc cgggtgtgat tcccgcgctg ctgcaactgg tggatgccgg
     8221 ctaccggctg gtgctggtca gcaaccagga cggccgtggc accgagtcct ttcccgaaca
     8281 ggatttccag cgtacccacg acttcatcat ggagctgttc gccagccagg ggatccgttt
     8341 cgaggcggaa ttcatctgcc cgcattttcc gcaagacggc tgcgcctgcc gcaagccggc
     8401 caccggcctg ctgacacgct tcatcgcgca gcatccgccg gatctcgacc gcagcttcgt
     8461 gctgggcgat cgcgacacgg acctggcttt cgccgagaac ctcgggctgg tcggcctgcg
     8521 ggtcggcgac ggcacggggg acagcctgac ctggccggag gcggcgcggc gcatcctggg
     8581 tggcctgcgg cgcgcgatcg tcacccgggg caccgccgag acgcgcattc gcgtcgaggt
     8641 ggatctcgac gacccgcacg ggccggtgcg cgccgcgacc ggcatcggct actacgacca
     8701 catgctcgag caggtcgcca agcatggtgg cttctcgatg gagctcgagt gcagcggcga
     8761 tctgcacgtc gacgagcacc acaccgtcga ggacgtggcg ctggcactgg gcgaggcgct
     8821 gcgccaggcg ctcggtacgc gccgcggcat ccgccgctat ggcttcgtat tgcccatgga
     8881 cgagacccgt gccgagctgt ccatcgatct cggcggccgg ccctacctgg tgttccacgg
     8941 cgaattccac cgcgagcgcg tcggcgagct gcccaccgag ctggtgccgc atttcttccg
     9001 ctcgctggcc gacagcctgc gcgcctcgat ccacgtgcgg ctcgacggcg agaacgatca
     9061 ccaccaggtg gagggcgcct tcaaggcgct gggccgcgca ctgcgcgagg ccttccgccg
     9121 cgagggcggc ggcatcccgt ccaccaaggg catgctggcg tgaccggcgg gccggtcatc
     9181 gtggattcgg gcggtgcgaa catcagctcg ctgcgcttcg cgctcggccg gctgggctgc
     9241 agcgcgccgc tcaccgccga tgccgggcgg atccgcggcg cgagtcacgt gttcctgccg
     9301 ggcgtcggcg ccgccgacga ggcgatgacg cggctggagc gcagtgggct tgccgacctg
     9361 gtgcgcggcc tgcgccagcc cgtgctcggt atctgtctcg gcatgcagct gctgttcgag
     9421 gcctcggacg aggacggcgg gcgcgcatgc ctcggcctca tcccagggtg cgtgcggcgc
     9481 attcccggcg gcccggaccg gccggtgccg cacatgggct ggaacctgac cagcctggcg
     9541 ccggacggcg cgctgtttgc cgggctgccg ggcgccgcgc atttctacta cgtgcacagc
     9601 tatgccgccc cccccggcga gcacaccgtg gcggtggcgg actatggcgg cgagttcacg
     9661 gcggtggtgt cctgcggcaa tttccacggc gcccagttcc acccggagcg ctcgggcccg
     9721 gccggcgcgc gcgtgctggc caacttcctc gcgctctgag cagcgctgcg aatgccgcac
     9781 atggacctga agcccattcc cgccatcgac ctcaaggccg gccgcgtggt gcgcctctac
     9841 cagggccgtt tcgaccaggt ctccgagtat caggccgacc ccgagtcgct ggcggcgcgc
     9901 tacttgtcgc tgggagtcga ctgggtgcac gtggtggacc tggacggcgc gcgcgacggc
     9961 gccggcggca atcgcgaggt catcgcgcgc atggccggcc tggcgcgcgg gcgcatccag
    10021 gtcggcggcg gcatccgctc gcgcgacgac gtcgcagcga tgctcgccac cggcgccggg
    10081 cgcgcggtca tcggcagcac ggccgtggaa cgcccggcgg aggtgtgcgg ctggttcggc
    10141 gagttcggcg cggagcgcct ggtgctggct ttcgacgtgc agctggacga atcaggcgtc
    10201 cgcagatgcc gaacgcgcgg ctggaccg
//
LOCUS       DAOMPG010000071        10165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2845_length_10165_cov_4.838773, whole genome shotgun sequence.
ACCESSION   DAOMPG010000071 DAOMPG010000000
VERSION     DAOMPG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_10165_cov_4.838773"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..972
                     /locus_tag="PLS34_05490"
     CDS             190..972
                     /locus_tag="PLS34_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP86956.1"
                     /translation="MPAPYVMSGHGRPVTALVTGASRGIGLAFVRHLAATGVARRVWA
                     GCRHPDSAGALATLAAEHDKVRPLALDVTDEDSLAAAAGGIGAEGEPLDLVINCAGLL
                     HRPDGPQPERRLDEVRADWLLESFAVNGAGPLLLAKHVEPLLPRRERAVFASLSARVG
                     SISDNRLGGWYAYRGAKAAQNMFLKTLSIELARRARGAICVALHPGTTDTELSRPFQG
                     GVPEAKLFSPARSADQLLRVIDRLTRADSGRFYAWNGERIDW"
     gene            complement(1029..1418)
                     /locus_tag="PLS34_05495"
     CDS             complement(1029..1418)
                     /locus_tag="PLS34_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HRP86957.1"
                     /translation="MTALSDDHFRDLPPRERRYDTPLGDGLVFSVFPNGTKCWVLVYS
                     ASGFARRRTLGLFPEMGVDTAREAALQARRILDVESELAQAGEPSAVAGRRGWLIGLI
                     EDRPYLSVALGLGFAALLGMSVVWLLG"
     gene            1544..2947
                     /locus_tag="PLS34_05500"
     CDS             1544..2947
                     /locus_tag="PLS34_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP86958.1"
                     /translation="MDQEMMRRPEPWLLAVAGVAWIALGAEGGLWTLVPAAVPGALLL
                     ASAGGTLLFPGDRLIPRAGATGALVGLIFAVLTLPFALLAGLLLAGLSAVAGICAARL
                     TAEDLELPDALPVPEMDLRLAAEIALDESVIGSLTTVMGVYGKPIQARVGEELEATLD
                     WLRAGGWLNEPASFHEPPPPLGPVAVASKRIAGLDCEIMRFESEFDPRRGAPGRDRWI
                     GYANNRNAEVRLVRQSRSRDWLICVHGLAMGHAQLDLRAMGAGWLHERGVNLAFPVLP
                     LHGPRAINPAISGAGFITGDVTNTLHALTQAAWDVRRLVGWLREEGAERIGLAGMSLG
                     GYTTALVASLEDEFDCVIAGIPAAEFGELTCYHASGRALAVAQEANITPERISVALTP
                     VAPLVLTPKIPRDQRFIFGALADRFVPPRQVETLWRHWEEPEIKWYAGGHLGFRFHAE
                     VRDFVDRALRATLLRPR"
     gene            2965..4278
                     /locus_tag="PLS34_05505"
     CDS             2965..4278
                     /locus_tag="PLS34_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRP86959.1"
                     /translation="MLNTAGEMLGLTVKGALRIGQTGRVLAGTGVGWLFGDRPPTPRL
                     VRQTFERLGATYVKLGQLIASSPSMFPEPWVTEFQHCLDQTTPLPWRTIRRALKEELG
                     DKLESEFLWVDPEPLASASIAQVHAARLKTGEDVVLKVRKPGVQKVIVTDLNLLHLVA
                     RVAERVTPGAAHASLAAIVEEIQNSMMEECDFLREAANIEAFREYLDETLNTQAVAPR
                     VYPQLSTARVLTMERFFGVPLTDAEGLRRCTDDPEELLLNALNTWFGSLMHCRTFHAD
                     LHAGNLMALRDGRIGFIDFGIVGRIREETWKAMFALGEGLPKGDFKAVAEGLATMGAT
                     RDGVDTARLATDLEALFRRLDLAPPAPGDAHGHSAYDEPPEDEDLNRALMDLVAVGRR
                     HGIRFPREFTLLVKQFLYFDRYIRMLAPDLALFGDERLVALPGRG"
     gene            complement(4286..4843)
                     /locus_tag="PLS34_05510"
     CDS             complement(4286..4843)
                     /locus_tag="PLS34_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRP86960.1"
                     /translation="MHKPLLAVALLLLMSASAHAQSGAWLDIALDEQGVVTEAVVVGD
                     VHPALVGPLRDWVTRQPFTPAEADGQAVPSTTSVWATYTLVEVDDGYELKVLGYQSCP
                     RPIRQKEPSYPRGELLAGKQGWVNLGFTVQPDGKPSDIRVVESSHRNFEKPALRAIAK
                     WRFKPKTVNGQAVATEVTQKIDFEL"
     gene            5012..5248
                     /locus_tag="PLS34_05515"
     CDS             5012..5248
                     /locus_tag="PLS34_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP86961.1"
                     /translation="MSIRINLDVELAKKKMSLTELSQQVGVSMTNLSLLKTGKVKAIR
                     FSTLEAICRVLDCQPGDLLEFVPDGAQGGEHHES"
     gene            5238..5846
                     /locus_tag="PLS34_05520"
     CDS             5238..5846
                     /locus_tag="PLS34_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86962.1"
                     /translation="MNREYALPGYIAVALALLFPVYWISRLGVGGASFEEAFHADVTR
                     LDAWDLLFVLIGVMEIYLYFSLRKLLQERLQGTLPAAVLLLMAIVVGVFHATVVFDLV
                     LAIGPELTPGMEDRLVVAAAVVAISGLFVYAVLGFALSIMLLLPGAESPPLLKLFAAL
                     LMACCVLQFTIVFGILNVALFPVVLLLLAIYFLRGGHQVEVV"
     gene            complement(5910..6200)
                     /locus_tag="PLS34_05525"
     CDS             complement(5910..6200)
                     /locus_tag="PLS34_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86963.1"
                     /translation="MRANFMQVSLPRAATGVAGVVVALLMVPLVAMQFTAEVNWSAGD
                     FVVAGSLLFAAGMTYVVAARRVRGTGQRLALASLVLALLAIVWAELAVGLFT"
     gene            complement(6205..6567)
                     /locus_tag="PLS34_05530"
     CDS             complement(6205..6567)
                     /locus_tag="PLS34_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018744683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP86964.1"
                     /translation="MTDAQHHHHEIDYIEFGVTDMAVSQRFYAAAFDWEFTDYGPEYA
                     GIRKRQGGEAGGLRLQAEVAPGGPLVILYSENLEQTLARVSAAGGRITQEIFEFPGGR
                     RFQFRDPSGNELAVWSDR"
     gene            complement(6668..7039)
                     /locus_tag="PLS34_05535"
     CDS             complement(6668..7039)
                     /locus_tag="PLS34_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP86965.1"
                     /translation="MELGAFSVSLAVKDLAASKAFYEKLGFTAMGGDQSQGWLILKNG
                     STVIGLFQGMFEDNILTFNPGWDQDAQPLASFTDVRDVQKFLDAQGVEITVRAEPDST
                     GPAHLVLSDPDGNVIMLDQHV"
     gene            complement(7127..7711)
                     /locus_tag="PLS34_05540"
     CDS             complement(7127..7711)
                     /locus_tag="PLS34_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HRP86966.1"
                     /translation="MHNFSCKCGAIRGHILDGSPSNRVRCYCSDCQAFGRYFGPEAGV
                     LDPQGGTEVIQVSQSRLRFDSGLEQLACLRLSPRGLLRWYARCCNTPLGNSLPNPKAS
                     LIGLVHSCMDHASLDRDFGGRVADVNTSSALGDSRPRQSGLARTIGRFLVMMSKDRLS
                     GRYRRSPLFTGDGRPVVEPHVLDRTRVDALKRSP"
     gene            complement(7735..8151)
                     /locus_tag="PLS34_05545"
     CDS             complement(7735..8151)
                     /locus_tag="PLS34_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86967.1"
                     /translation="MFKPRRIESDPAWSDPDGIKIYTISARNQPVDQVPFLQRLAEVK
                     QLSTVRWATAPAFAIFHEGSSLAYLILAWWGNDNELFTSVSARTASGWVEDAAAYSFC
                     IYDLEIFWHERNYFVEHVFCAAPSLANYRARRFRNG"
     gene            complement(8215..8877)
                     /locus_tag="PLS34_05550"
     CDS             complement(8215..8877)
                     /locus_tag="PLS34_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRP86968.1"
                     /translation="MTRVRVESFTVSLDGYGAGPDQDIDNPLGVGGRDLHQWAMPTRT
                     FQRAVFGRDGGTTGIDEDFAARGFRDIGAWILGRNMFAPTRGPWTDLGWKGWWGDDPP
                     YHVPTFVLTHHPRESIPMAGGTTFHFVTGGIHEALDRAGEAAAGKDVRIGGGPDTIRQ
                     YLRASLIDELHIAIAPMLLGRGESLWEGLDLPALGYTCVECVASEKATHVVLRHHGSS
                     AA"
     gene            complement(8904..9242)
                     /locus_tag="PLS34_05555"
     CDS             complement(8904..9242)
                     /locus_tag="PLS34_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRP86969.1"
                     /translation="MNPITWDDFVQVELRVGIVTSASPFPEARKPAYILNVDFGPDIG
                     IRKSSVQITALYTPEELVGRQVVAVVNFPKKQIGPIMSECLVTGFHNEHGEVVLCVPD
                     TPVPLGTKLL"
     gene            complement(9220..9930)
                     /locus_tag="PLS34_05560"
     CDS             complement(9220..9930)
                     /locus_tag="PLS34_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRP86970.1"
                     /translation="MLRLHIANKNYSSWSLRPWILLRELEIPFEEQLTPFGDGNGTSS
                     FAAFSPSGRVPCLVHEELTVWDSLAIIEYLAEADPRVWPIQRERRAWARSVAAEMHSG
                     FSHVRNICGMNCGLRVQMHQWSAPLLAEWRRIDAIWCEGIARFGGPFLAGTQFSAADA
                     FFAPVAFRVQSYSPRLSPDAQAYARRLLALPHMVDWYESALQETWRDEAHDQEARAVG
                     TVIQDLRPGGPDEPDHMG"
     gene            complement(9979..>10165)
                     /locus_tag="PLS34_05565"
     CDS             complement(9979..>10165)
                     /locus_tag="PLS34_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86971.1"
                     /translation="ANGWAKLVIERDALLALRDRGALASEYPGNGMLTAHKAGPPDFV
                     HLDWAPESPVDAVEPRD"
BASE COUNT         1622 a         3422 c         3425 g         1696 t
ORIGIN      
        1 atgcggcgca tggcgaggtc cgggtctgaa tcgatgccgc tagcgtacct ccgcagcccg
       61 tgtttcacac gcgccggggc gcgccgcctg tggctgtatc cgttccgccc accggctcga
      121 acacggccgg tatccacctg cgcgagccgc cgccacgcca tggccaacgc cccgtcctct
      181 ccggccccga tgcccgcccc ctatgtcatg tctggccatg gacgcccggt gaccgcgctg
      241 gtgaccggcg ccagccgcgg gatcggcctg gcctttgtgc gtcatctggc cgccacgggc
      301 gttgcgcgcc gcgtctgggc gggctgccgc cacccggatt ccgcgggtgc gctggccacg
      361 ctggcggcgg aacacgacaa ggtccgtccg ctggcgctgg acgtcaccga cgaggacagc
      421 ctggcggccg cggccggcgg catcggcgcg gagggcgagc cgctcgacct ggtcatcaac
      481 tgcgcggggc tgctgcatcg cccggacggc ccacagcccg agcggcggct ggacgaggtg
      541 cgcgccgact ggttgctgga gtccttcgcg gtgaacggtg ccgggccgct gctgctcgcc
      601 aagcacgtcg agccactgtt gccgcggcgc gagcgcgccg tgtttgccag cctgtcggcg
      661 cgcgtgggca gcatcagcga caatcgcctg ggcggctggt atgcgtaccg cggcgccaag
      721 gcggcacaaa acatgttcct gaagacgctg tccatcgagc tggcgcgtcg cgcgcgcggc
      781 gccatctgcg tggcgctgca cccgggcacg accgacaccg aactgtcgcg gccgttccag
      841 ggcggcgtcc ccgaagccaa gctgttcagc ccggcgcggt ctgcggacca gctgttgcgc
      901 gtcatcgacc ggctcacgcg ggcggacagc gggcgcttct acgcctggaa cggcgagcgg
      961 atcgattggt gagccgggcg ggtgcgccag cgggttcggg cccgaacgcg aaccgtcgat
     1021 gcggctgttc agcccagcag ccagaccacc gacatcccga gcagggcggc gaagcccagt
     1081 ccaagtgcga cggacaggta cggccggtcc tcgatcagcc cgatgagcca gccgcgccgc
     1141 ccggccacgg cgctcggctc gccggcctgc gcgagctcgc tttccacatc aaggatgcgg
     1201 cgtgcctgca gcgccgcctc gcgcgccgtg tccacgccca tctcgggaaa caggccgagc
     1261 gtgcgccggc gcgcgaagcc gctcgccgag tacaccagca cccagcactt ggtgccgtta
     1321 gggaacaccg agaacacgag cccgtcgccg agcggcgtgt catagcggcg ttccctcggc
     1381 ggcaagtcgc gaaagtggtc gtcgctcagc gcggtcatgg gattccttcc acagctcgac
     1441 cagtcacatc ggtcattgag aataccttat gcttggagac cgggcgacgg cgccttccgc
     1501 gcgcgggccc cggaaattca aggatcgcga cagggatgcg ggcatggacc aggaaatgat
     1561 gcgcaggccg gagccctggc tgctcgccgt ggcgggcgtg gcctggatcg cgttgggcgc
     1621 cgaaggcggc ctctggacgc tggtgccggc ggcggtgccg ggtgcgctgc tgctggccag
     1681 cgcgggtggc acgctgttgt tcccgggcga ccggctgatc ccgcgcgcgg gcgccaccgg
     1741 cgcgctagtc gggctgattt tcgctgtgct gacgctgccg ttcgcgctgc tggcggggct
     1801 gttgctcgcc ggcttgtcgg cggtggcggg catctgtgcg gcgcggctca cggccgaaga
     1861 cctggagctg cccgacgcgc tgcccgtccc ggaaatggac ctcaggctgg cggccgagat
     1921 cgcgctcgac gagtccgtga tcggcagcct cacgacagtg atgggggtgt acggcaaacc
     1981 catccaggcg cgtgtcggcg aggagctcga ggcgacgctc gactggctgc gcgccggcgg
     2041 ctggctcaac gagccggcga gtttccacga gccgccgccg ccgctcggtc cggtggccgt
     2101 cgccagcaag cgcatcgccg ggctggactg cgagatcatg cgtttcgaga gcgagttcga
     2161 cccgcggcgc ggcgcaccgg ggcgcgatcg ctggatcggc tacgccaaca atcgcaacgc
     2221 cgaggtgcgg ctggtgcgcc agtcacgcag ccgcgactgg ctgatctgcg tccacggcct
     2281 ggcgatgggc catgcccagc tcgacctgcg cgccatgggc gcgggctggc tgcacgagcg
     2341 cggcgtcaac ctggccttcc cggtgttgcc gctgcacggc ccgcgcgcca tcaatccggc
     2401 catcagcggc gccggcttca tcaccggcga tgtgaccaac accctgcacg cgctcaccca
     2461 ggcggcctgg gacgtccgtc ggctggtcgg ctggctgcgg gaagaggggg ccgagcgcat
     2521 cggcctggcc ggcatgtcgt tgggcggcta caccacggcc ctcgtggctt cgctggagga
     2581 cgagttcgac tgcgtcatcg ccggcatccc ggcggccgag ttcggcgaac tgacctgtta
     2641 ccacgccagc ggccgcgcac tcgccgtcgc gcaggaggcg aacatcacgc cggagcgcat
     2701 cagcgtcgcg ctgacgccgg tcgcgccgct ggtgctcacg ccgaagatcc cgcgcgacca
     2761 gcgcttcatc ttcggcgcgc tggccgaccg cttcgtaccg ccgcgccagg tggagacgct
     2821 gtggcgccac tgggaggagc ccgagatcaa gtggtacgcg ggcgggcacc tcggtttccg
     2881 cttccatgcc gaagtgcggg acttcgtgga ccgcgcgttg cgcgccacgc tgttgcggcc
     2941 acgctgaatc gacggggaac cgggatgctg aacaccgccg gcgagatgct cggcctgacg
     3001 gtcaagggcg cgttgcggat cggccagacc ggccgcgtgc tggccggcac cggcgtgggc
     3061 tggctgttcg gcgatcgtcc gcccacgccg cgcctggtgc gccagacctt cgagcggctc
     3121 ggcgccacct acgtcaagct cggccagctg atcgcgagct cgccctcgat gttccccgag
     3181 ccctgggtca ccgagttcca gcattgcctc gaccagacca cgccgctgcc ctggcgcacc
     3241 atccgtcggg ctctgaaaga ggaactgggc gacaagctcg agagcgagtt cctgtgggtg
     3301 gacccggagc cgctcgcctc ggcctccatc gcccaggtgc acgccgcgcg gctgaagacc
     3361 ggcgaggacg tcgtgctcaa ggtgcgcaag ccgggcgtgc agaaagtcat cgtcaccgac
     3421 ctgaacctgc tgcacctcgt cgcgcgcgtg gccgagcggg tcacgccagg cgcggcgcat
     3481 gcctcgctcg ccgccatcgt cgaggaaatc cagaactcga tgatggagga gtgcgacttt
     3541 ctcagggagg ccgccaacat cgaggccttc cgcgagtacc tcgacgagac gctcaacacc
     3601 caggcggtcg cgccgcgggt atacccgcag ctgagcaccg cgcgcgtgct gaccatggag
     3661 cgcttcttcg gcgtgccgct gaccgacgcc gagggcctgc gccgctgcac cgacgatcct
     3721 gaagagctgc tgctgaacgc gctcaacacc tggttcggca gcctgatgca ctgccgcacc
     3781 ttccacgccg acctgcacgc cggcaacctg atggcgctgc gcgacgggcg catcggcttc
     3841 atcgacttcg gcatcgtcgg gcgcatccgc gaggagacct ggaaggcgat gttcgcgctc
     3901 ggcgagggcc tgccgaaggg cgatttcaag gcggtcgccg aggggctcgc gaccatgggc
     3961 gcaacccgcg acggcgtgga cacggcgcgc ctcgcgaccg acctcgaggc gctgttcaga
     4021 cggctggacc tggcgccgcc ggcaccgggc gacgcgcacg gccactcagc ctacgacgag
     4081 ccacccgagg acgaggacct caaccgcgcg ctcatggacc tggtggccgt cgggcggcgc
     4141 cacggcatcc gcttcccgcg cgagttcacg ctgctggtaa agcaattcct ctatttcgac
     4201 cgctacatcc gcatgctggc accggatctg gcgctgttcg gcgacgagcg gctggtggcc
     4261 ttgccgggcc ggggttgacg cgctactaga gctcgaagtc tatcttctgg gtgacttcgg
     4321 tggcgaccgc ctggccgttc acggtcttgg gcttgaagcg ccatttcgca atggcgcgca
     4381 gggcgggttt ctcgaagttg cggtgcgagg actccaccac ccggatgtcg ctgggcttgc
     4441 cgtccggttg cacggtgaag ccgagattga cccagccctg ctttccggcc agcaattcac
     4501 cacggggata gctcggctcc ttctggcgga tcggccgcgg gcagctctgg tatcccagca
     4561 cctttagttc gtagccgtcg tccacttcca ccagcgtgta ggtcgcccac accgaggtcg
     4621 tgctgggcac tgcttgtccg tccgcctccg cgggcgtgaa cggctggcgc gtcacccagt
     4681 cgcgcagcgg acccaccagc gcgggatgca cgtcgccgac cacgaccgcc tcggtcacaa
     4741 ctccttgttc atccagcgca atgtccagcc aggcgccgga ctgggcatgg gccgacgcgg
     4801 acatgagcag cagcaacgca acggccagca gtggcttgtg catgggttcg gtccctccaa
     4861 gtgattcgcg cagcgcctgc agtataaccg gcgccggggc caggacgagg ccgcggatcg
     4921 gccgggctgt tgacatgtta tccgtaaaat gtattatgtg tgtcattaaa tataagatgt
     4981 aaatcataaa gagcatggca ggcccatcat catgagcatc cgcattaacc tcgatgtcga
     5041 gctggccaag aagaaaatgt cgctgacgga gttgtcgcag caggtcggcg tgtcgatgac
     5101 caacctgtcg ttgctcaaga caggcaaggt caaggcgatc cgcttcagca ccctcgaggc
     5161 gatctgccgg gtgctcgact gccagcccgg cgacctgctc gaattcgttc ccgacggcgc
     5221 ccaaggaggc gagcaccatg aatcgtgagt acgcactgcc cggctacatc gcagtcgctc
     5281 ttgccctgtt gttcccggtc tactggattt ccaggctcgg cgtcggcggg gcttctttcg
     5341 aggaagcctt ccatgcggac gtcacccggc tcgacgcctg ggatctgctg ttcgtgctga
     5401 tcggcgtgat ggagatctat ctctacttca gcctccgcaa gctgctgcag gagcgactgc
     5461 aaggcacgct gcccgcggcc gtcctgctgc tgatggcgat cgtcgtcggc gtgttccacg
     5521 ccaccgtggt gttcgacctg gtgctcgcca tcggcccgga gttgaccccg ggcatggagg
     5581 atcgcctggt agtggccgcc gcggtggtcg caatctcggg gctgttcgtc tacgcggtgc
     5641 tcggcttcgc gctgtcgatc atgctgctgc tgcccggggc cgagtcaccg ccgctgctga
     5701 agctgtttgc ggcgctgttg atggcctgct gcgtgctgca gttcaccatc gtgttcggca
     5761 ttctgaacgt ggcgctcttc ccggtcgtcc tgctgctgct ggcgatctat ttcctgcgcg
     5821 ggggtcacca ggtggaggtg gtgtgacggc cccacatcgg cgggaggtgg tcaccaccag
     5881 attggcgcgc cccggccaca gccggatgac taggtgaaca ggccgacggc aagctcggcc
     5941 cagacgatgg ccagcagcgc caggacgagc gatgcaaggg ccaggcgctg ccctgtcccc
     6001 cggacacgcc gggcggccac cacgtaagtc attcccgcgg caaacaggag cgacccggcg
     6061 acgacaaagt cgcctgcact ccagttgacc tccgccgtga attgcatggc gaccagcggc
     6121 accatcagga gggcgacgac cacgcccgcg acgccggttg ctgcacgcgg gagggaaacc
     6181 tgcatgaagt tggctctcat gcggtcaccg gtccgaccag acggccagct cattgccgct
     6241 ggggtccctg aactggaagc gccggccgcc gggaaactcg aatatttcct gcgtgatgcg
     6301 gccacccgcg gcgctgaccc tggccagcgt ctgctcgagg ttctcggagt acaggatgac
     6361 cagtggaccg cccggcgcca cttccgcctg tagccgcagc ccgccggctt cgccgccctg
     6421 gcgcttgcgg atgcccgcgt actcgggacc gtaatccgtg aactcccagt cgaatgcggc
     6481 cgcgtagaag cgctgcgaca cggccatgtc ggtcacaccg aactcgatgt agtcgatctc
     6541 gtggtggtgg tgttgtgcgt ccgtcatcgt cgtgcgattg tacctgccgt cgaaggcccg
     6601 gcgttagatg ctgtcgcaag tctgcaaaag aacatgcgtc ggtcggccgg gaccctgaaa
     6661 ccgaaggtca aacatgctgg tccagcatga ttacgttgcc gtctgggtcg gaaagaacga
     6721 ggtgtgcggg gccggtgctg tccggttctg cccggaccgt gatctccacc ccctgcgcgt
     6781 cgagaaactt ctgcacgtcg cggacatccg tgaaagacgc caatggctgg gcgtcctggt
     6841 cccagcccgg attgaaagtg aggatgttgt cttcgaacat gccctggaac aggccgatga
     6901 ccgtcgagcc gtttttcagg atcagccagc cctgcgactg gtcgccgccc atggcggtga
     6961 aaccgagctt ttcgtagaag gccttcgacg ccgcgaggtc tttaacagcg aggctgacag
     7021 agaatgcacc gagttccatt gctcccctcc cgttcgaaga atactgagtg actaacctgc
     7081 tgcaccgtga ccggcgccgc ctgagtgccg ccggtcgggc agcatgtcag gggctcctct
     7141 tcagggcatc gactcgtgtc cggtccagca cgtgcggctc gaccacgggc cggccgtcgc
     7201 ccgtgaagag cggtgagcgt cgatagcgcc cggaaagccg gtccttgctc atcatgacca
     7261 ggaagcgccc gatcgtccgg gccagcccgc tctgccgggg cctggagtca ccgagcgctg
     7321 aactcgtatt gacgtccgcc acgcgaccgc cgaagtcccg gtccagggac gcatgatcca
     7381 tgcaggagtg gacgaggccg atcagcgagg ccttagggtt cggcagggag ttgcccagcg
     7441 gcgtgttgca acagcgggcg taccagcgca gcaggccgcg cggcgaaagc cgcaggcagg
     7501 ccagctgttc gagcccgctg tcgaagcgca ggcgtgattg ggagacctgg atgacttcag
     7561 tgccgccttg cgggtcgagc acgccggcct cgggaccgaa gtaacgaccg aaggcctggc
     7621 agtcgctgca gtaacagcga acccggttgc tgggactgcc gtcgaggatg tggccgcgga
     7681 tggcgccaca cttgcagctg aagttatgca tgacaggcct tcatcagcgc cagtctatcc
     7741 gttccggaac cgcctggcgc gatagttcgc caggctgggc gcggcacaaa acacgtgctc
     7801 cacgaaatag ttgcgctcgt gccagaaaat ttcgaggtca tagatgcaga acgaataggc
     7861 cgcggcatcc tcgacccagc cggaggccgt cctggctgag accgacgtga acaactcgtt
     7921 gtcattgccc caccaggcga ggatgagata ggcgagactc gagccctcgt gaaagatggc
     7981 gaaggccggc gccgtcgccc agcgcaccgt gctgagttgc ttgacctcgg caaggcgctg
     8041 caggaacggc acctggtcga ccggctggtt gcgtgccgaa atcgtgtaga tcttgattcc
     8101 gtcagggtcg gaccacgccg gatcgctctc gattctcctg ggtttgaaca tcgcggggct
     8161 cagaattcct ggtgtacttg cggatcgcgg aaggccactg ggtcgaccgg gcggtcaggc
     8221 ggccgagctc ccgtggtgcc gcagcacgac atgcgtcgcc ttctccgatg ccacgcactc
     8281 gacacaagtg tatcccagtg ccggcaggtc gagcccttcc cacaggcttt cgccccggcc
     8341 gagcagcatc ggcgcgatgg cgatgtggag ttcatcgatc aggctcgcgc gaagatactg
     8401 ccggatggtg tcggggccgc cgccgatgcg cacgtccttg ccggcagccg cctcgccggc
     8461 cctgtcgagc gcttcgtgga ttccgcccgt gacgaaatgg aacgtcgtac cgcctgccat
     8521 tggaatggat tcgcgggggt gatgcgtcag cacgaaggtc ggcacatggt acgggggatc
     8581 gtcgccccac cagcctttcc agcccaggtc ggtccagggc ccgcgcgtcg gcgcgaacat
     8641 gttccgccca agaatccagg cgccgatgtc tcgaaagccg cgggcggcaa aatcctcgtc
     8701 gattccggtg gtgccgccgt ccctgccgaa cacggcccgc tggaacgtac gcgtcggcat
     8761 ggcccactga tgcaggtccc ggccgcccac gccgagcggg ttgtcgatgt cctggtccgg
     8821 accggcgccg tagccgtcca gcgagacggt gaagctctct acgcgaactc gtgtcactgc
     8881 ctgcctcgca tccgctgcaa ttcttacagc aatttcgtgc cgagcggaac cggcgtatcc
     8941 ggaacacaca gcaccacttc tccgtgctcg ttgtggaagc cggtcaccag gcactccgac
     9001 atgatcggcc cgatctgctt cttcgggaaa ttgaccaccg ccaccacctg gcgccccacc
     9061 agttcctcgg gcgtgtacag ggcggtgatc tggacactgg acttcctgat gccgatgtcc
     9121 ggcccgaagt cgacgttcag gatgtaagca ggcttcctgg cctctggaaa aggcgaagca
     9181 ctcgtgacga taccgacacg aagttccacc tggacgaagt catcccatgt gatcgggttc
     9241 atcggggcct ccggggcgca ggtcctggat caccgtgccc accgcgcgcg cttcctggtc
     9301 atgcgcctcg tcccgccagg tctcctgcaa cgcgctctcg taccagtcga ccatatgagg
     9361 cagcgccagc aggcgccgtg cgtaggcctg tgcgtccggt gagagccggg gcgagtagct
     9421 ttgcacgcgg aacgccaccg gcgcaaagaa cgcatccgcc gcggagaatt gcgttcccgc
     9481 caggaagggc ccgccgaacc gggcgatgcc ttcgcaccag atcgcgtcga tgcgtcgcca
     9541 ttccgcgagc aggggcgccg accactggtg catctggacc cggagcccgc agttcatgcc
     9601 gcagatgttc cggacatggg agaaacccga gtgcatctcg gcggccacgc tccgggccca
     9661 ggctcgccgt tcccgctgta tcggccagac ccgtggatcg gcctcggcga gatactcgat
     9721 gatggccagc gagtcccata cggtcagctc ctcatgcacc aggcagggta cccggccgga
     9781 gggcgagaag gcggcgaacg aggacgtgcc gttgccatca ccaaagggcg tcagttgctc
     9841 ctcgaagggg atctcgagtt ctcgcagcag tatccacggc cgcagcgacc atgaggagta
     9901 gttcttgttg gcaatgtgga ggcggagcat gcctggtcac ctgttgttga aagtccatgc
     9961 tgccgggtcg ccccgcaatc agtcccttgg ctccactgca tcgacggggc tctcgggcgc
    10021 ccaatcgagg tggacgaagt caggcgggcc ggccttgtgg gcggtcagca taccgtttcc
    10081 aggatactcc gatgcaaggg cgcctcggtc cctgagcgcc agcagcgcgt ctcgctcgat
    10141 gaccagcttt gcccagccgt ttgcg
//
LOCUS       DAOMPG010000072        10138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2855_length_10138_cov_4.675593, whole genome shotgun sequence.
ACCESSION   DAOMPG010000072 DAOMPG010000000
VERSION     DAOMPG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_10138_cov_4.675593"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLS34_05570"
     CDS             <1..713
                     /locus_tag="PLS34_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86972.1"
                     /translation="SLDQNDSTNFDVTWTDGVWWQWRGQDWTTHVIARHNDRRGSTGG
                     LRRPLDFRDSDSRAGLFLATESKTAIGPLVQRMVTLTWLPSALRSNGLANPALEDYLA
                     VTAKATAEWPLGEGGTKFRLGGEIGWAADTPRREVMNSGTGDADAFSWQTSFNFMDVL
                     PDHDLGIVYGRIADGWLLSSDFRPNDELLEVRWVWQATKAWQLDARFRRREEIDLPAS
                     AARPRRDDDIYLRATWRF"
     gene            867..1916
                     /locus_tag="PLS34_05575"
     CDS             867..1916
                     /locus_tag="PLS34_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86973.1"
                     /translation="MTPGSRLLASVVTACMSTLAPAQAGELLYTLADTATLPSTDTGW
                     DYIKMEPKSSRLFMARDKDGLTVFDVDANRAVTTVDDSVGANGPLLLPEYNRGYVAMT
                     DGSFLSFELDSLKPIARLQLDGDGGINSATHDPLTRRIHAIVGTRPAESTWYTLDAAS
                     GKLLGSKTFPFRKMDDPATDGKGRLFAPARRDNLILVLDSETLDEKTRWTVPCNVSKV
                     RYQAHSNRILGACAGDNPMFFALDPDSGRVVASLPIGNGIDGFAIDEQRKRIVTSNYE
                     GTLTVINQEGPDRYTLAGTVSTRPNARMMTMDERTGRLFVVTAELTRSPPDANGATTN
                     TYHPDSFVVLTYRPQ"
     gene            complement(2092..3030)
                     /locus_tag="PLS34_05580"
     CDS             complement(2092..3030)
                     /locus_tag="PLS34_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRP86974.1"
                     /translation="MKTIPASPVSAARSGQPDRATAWRRRSKLACLLLAAGIAGSACA
                     ADPADAPPFAITGGRDCADCPEMIVLPAGSFLMGSADYEQGHEANETPLHEVTFVEPF
                     AIGKYEITYDQWDACVADGACEAVADEGWGRGSRPVLYVNFDMAVGYTRWLSRKTGQR
                     YGLPSEAQWEYAARGGTATPWFWGDDPTLACEFGNVGDDALRQVRPDWPLHDCNDGHA
                     RITTPVGSFKPNGFDLYDTAGNVWEWVEDCYNPSYEGAPGDGSAWLTGDCVRRVVRGG
                     GWYNKPETVRPAMRYAGDDPKRQNNTLGFRVVRSLP"
     gene            complement(3137..6514)
                     /locus_tag="PLS34_05585"
     CDS             complement(3137..6514)
                     /locus_tag="PLS34_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86975.1"
                     /translation="MTRERPRLPALAIALLLPLSSWAHEGHGHAGLPPWLSQGPAFEQ
                     ADAVGQPGQTLPMAPGRRIAFDTDQGTWLSPDVSPDGRRIVFELLGDLYTMPAAGGTA
                     RAITRGLAFDSQPVFSPDGRRIAFLSDRSGAENLWLVDADGRAPRQLTLLDGNPIFAS
                     PAWSADGRALFVSRYRPELEAWELWRFDAAHGDGELLLPIRERYDQPPATWRSALGAA
                     PSADGRFVYYAAHVGPRETGRTPEWTIRRRDLRDGSEETLVSAPRSPRPDLVLGTAMR
                     PAVSPDGRWLVYGARHQARTGLRVLDLQTRADRWLAFPVQQDELGASHWRDLLPRHAF
                     TPDGSALVVNVDGGLQRIALADGTRQAIPFRARVDIELGAATRQSIRQDTGPVRARLI
                     QTPEPSPDGARLAFSALGALYVMPLDGSGTARRIDTGTRPAFHPSWSPDGRELAYIDW
                     TAKDAGHVWRIALDAPGSKPRQVSSTPAFHTRPVFTPDGTRIVAIRSSNSVRMHSYME
                     YGTLRDGELVVFERDGGDARVVASGRLGGRPHFDGDASRVHVLSGGGLERVALDGSGQ
                     DTVLQVTGPGWYFAAGRAPVDDLRISPDGRWALAQIAQQLHLLAMPEDAGGTLDLAAP
                     GVAHAKLTTVGADFFGWSDGGRTLHWAVGSTWYRRPLDGVRTHPADAPSMAVDMPVDG
                     RDGVQAFAAVVEVPRDTPRGALLLRGATALTMRGDEAVVDADLLVVDDRIAAVGKRGS
                     VAVPDGATVLDVSGRYLIPGLIDTHTHIADIRRDVLDLESWGPLANLAYGVTTVFDPS
                     TLSIDTLAYQDLVEAGRMPGARIFSTGPAIFSFNEFTSYEQVRDVLRRHRGHYRLGNI
                     KMYRTGNRRVRQWVAMAARETGLMPTTEGALSMKLGLTHALDGYAGNEHALVAVPLQR
                     DVVRLFADAGTAYSATLMIGNGGPEGQDDFISRGGLAHDRKLRRFAPGFIVDMKTRSR
                     TWREPDEYLYPRVAAGVAEVVRAGGVVGMGSHGEMPGIGLHWEMQAHAMGGMSNAEVL
                     LAATLGGAATIGRDAEFGSLEAGKFADLVVLAKDPRSDLANALSIVQVMKNGRLLDGD
                     TLHERWPTPHPLPRPWHWDDRPPGTPDPGAPVHEATP"
     gene            complement(6511..7740)
                     /locus_tag="PLS34_05590"
     CDS             complement(6511..7740)
                     /locus_tag="PLS34_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86976.1"
                     /translation="MRWLVLALACLLPAARAADTVAIVHARAHPVSAPVVEDATVVLR
                     DGRIVSVEAGGAAPQGARIVDAGGRDLTPGLFHAATRIGLSEVGDGQEAVASGKSRAG
                     YTTHYAIDANAQSVEQARADGVSRALVAPAAAADDVFAGRATLLHLRTGGDIVERSGD
                     VVQFAYIGTDASRAHSHAAAWQRLHKKLGRGRTPPHHADGLVHVDADDIAQHEVAMRR
                     TPLAIVADREADIREAIALARTLGLRVVVVGGAEAWRLAPELAAAGIAVVLDPLDALP
                     ATYDRLGARHDNAALLHRAGVDIAFTASAQGIYTSWNAGPSLRLAAGIAAAHGLPDDA
                     ALAAITQAPARIWGLDARIGTLAPGADADLVLWDGDPLEPSSAPVAVFVAGDAVPLRT
                     RQTLLRDRYLPARTRAP"
     gene            complement(7740..9095)
                     /locus_tag="PLS34_05595"
     CDS             complement(7740..9095)
                     /locus_tag="PLS34_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP86977.1"
                     /translation="MARVRIPAFLSLALFAASLLVAASATSAADADPYASRYVPADAP
                     PTLFAGATVLDGAGARLNGHDVLVEGGRITEIGRDLPRQPGVRVVEAGGAWLTPGLID
                     VHTHLGTFPQPYEHGRHAFADVTETSRSRTPDIWIEHAVRASDPAFQHALAAGVTTLQ
                     VLPGSSSVIAGRGVVLKPVRAVTVVEMKFPGAPQGLKLACGSNPSLSGDGRPGFPDSR
                     MGVIGELRAALGTARARMRAGGRDPVPDLVQDTLALALRGEMPVHVHCYRADDIATVL
                     AVADEFGLRIAAVHHASEAYKIAGLLREHGTCAAVWSDWWGFKREAEDGIRENAAIVD
                     AAGGCAIMHSDIPVLGSLLNLEVAKAAAAGRRAGLDIPPERAIRWITSNAAQALGLGD
                     RIGRIAHGYGADLVLWSGDPFSVRSRPRLVMIDGVVQVEDGARPLSDFELGRAAMGQG
                     P"
     gene            complement(9100..9693)
                     /locus_tag="PLS34_05600"
     CDS             complement(9100..9693)
                     /locus_tag="PLS34_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRP86978.1"
                     /translation="MSTPFDPRSPADVLALVEAFPLAWVIAHEGDAPATPLPLLAECD
                     AGGAIVSLFGHLARRNPLCAALQAAPRALLLFQGPQAYVAPRLVSKPDWGPTWNYAVA
                     RFQVRIEFAPEETDAALARLAAHLEAGQAEPWTTERMGARYAQLREHIIAFRAHVEHA
                     HATFKLGQDETSQAFAEIVAGHRDPVLVDWMHRLSEG"
     gene            complement(9690..>10138)
                     /locus_tag="PLS34_05605"
     CDS             complement(9690..>10138)
                     /locus_tag="PLS34_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRP86979.1"
                     /translation="VASQLRLRDLGRPPVAAMLLNYGAYGDRTSPSWARYDGPTYMLE
                     ADEMHRFWDNYLRGDGDRDDPLAMPLLADLRGLPPAFLAIAECDILADGNREMAAALH
                     DAGVPVQARTYAGTTHSFLEAMSISDLADRALDEASDWLRRTLLAP"
BASE COUNT         1443 a         3614 c         3685 g         1396 t
ORIGIN      
        1 agtcgctcga ccagaacgac agcaccaact tcgacgtcac ctggaccgac ggcgtctggt
       61 ggcagtggcg cggccaggac tggaccacgc acgtgatcgc ccgccacaac gaccgtcgcg
      121 gctccactgg cggcctgcgc cgcccgctgg acttccgcga cagtgactcg cgcgccggcc
      181 tgttcctcgc caccgaatca aagaccgcga tcggcccgct ggtgcagcgc atggtgacgc
      241 tgacctggct gccgtcggcg ctgcgcagca acggcctcgc caatccggcg ctggaggact
      301 acctggcggt caccgccaag gccaccgccg agtggccgct gggcgaaggc ggcacgaagt
      361 tccggctcgg cggcgaaatc ggctgggcag cggacacacc gcgccgcgag gtcatgaact
      421 ccggcaccgg cgacgccgac gcattctcct ggcagacctc gttcaacttc atggacgtgc
      481 tgcccgacca cgatctcggc atcgtctacg gccgtatcgc cgacggctgg ctgctgtcga
      541 gcgacttccg gcccaacgac gagctgctgg aagtgcgctg ggtctggcag gcgacgaagg
      601 cctggcagct cgacgcccgt ttccgccggc gcgaggaaat cgatctgccg gccagtgccg
      661 cccgcccgcg ccgcgacgac gacatctacc tgcgtgccac ctggcgcttc tgaccggagc
      721 tgccgcgagt cggctccgaa tggagaagcc gccgtgcgcc gcaggcggca agctgcatgg
      781 tcgctgcctt cacatcggga ggatgtccgc ccgatgacgt gctgcaggcc gtttccgtgc
      841 atcttcaata tcgagggagt tattcgatga cgccaggttc ccgtttgctc gcatccgtcg
      901 ttacggcctg catgtccacg ctcgcgccgg cgcaggccgg cgagctgctc tacacgctcg
      961 ccgacaccgc caccctgccc agcaccgata ccggctggga ctacatcaag atggagccca
     1021 agagcagtcg cctgttcatg gcgcgcgaca aggacgggct gaccgtgttc gatgtcgacg
     1081 ccaaccgggc ggtgaccacc gtcgacgact ccgtcggcgc caacgggccg ctgctgctgc
     1141 cggagtacaa ccgcggctac gtcgccatga ccgacggctc gttcctcagc ttcgagttgg
     1201 attcgctcaa gcccatcgcg cggctgcagc tggacggcga cggcggcatc aacagcgcca
     1261 cccacgatcc gctcacccgg cgcatccatg ccatcgtcgg cacccggccc gcggaatcga
     1321 cttggtacac gctcgatgcg gccagcggca agctgctcgg cagcaagacc tttccgttcc
     1381 ggaagatgga cgaccccgcg accgacggca agggccgcct gttcgcgccg gcgcggcggg
     1441 acaacctgat cctggtgctg gattcggaga cgctggacga gaagacccgc tggacggtgc
     1501 cgtgcaacgt ctccaaggtc cgctatcagg cgcacagcaa ccgcatccta ggcgcctgcg
     1561 ccggcgacaa ccctatgttc ttcgcactgg acccggacag cggccgcgtc gtggccagcc
     1621 tgccgatcgg caacggcatc gacggtttcg ccatcgacga gcagcgcaag cgcatcgtga
     1681 cctcgaacta cgaaggcacg ctgacggtga tcaaccagga aggccccgac agatacacgc
     1741 tggccggcac cgtctccacc cgtcccaacg cgcgcatgat gacgatggac gaacggaccg
     1801 gccgactgtt cgtggtcacc gccgagctca cccgcagtcc tccggacgcc aacggcgcga
     1861 ccaccaatac ctaccacccc gacagcttcg tagtgctgac ctaccggcca cagtgatcgg
     1921 cacttccgaa tgcaggcgca cccgacagga tgtcttcccg agcgaagcgc gggatctcat
     1981 ctggtgcccg ggcgctacgc gcggaaacgg acccccgccg cgccggagat gacggccatc
     2041 cacgtctgaa cgcaagcgga tcccgccccc cccggatggc gggcaacgcc atcagggcag
     2101 cgagcggacg acgcggaacc ccagcgtgtt gttctgccgt ttcgggtcgt caccggcgta
     2161 gcgcattgcc gggcggaccg tttcgggctt gttgtaccag ccgccgccgc ggacgacccg
     2221 gcgcacgcag tccccggtga gccaagcgct gccgtcaccg ggcgcgcctt cataactggg
     2281 gttgtagcag tcctccaccc actcccagac gttgccggcc gtgtcgtaca gatcgaagcc
     2341 gttgggcttg aagctgccca cgggggtcgt gatcctggcg tggccgtcgt tgcagtcgtg
     2401 cagcggccag tccgggcgca cttgcctgag cgcatcgtcg cccacgttgc cgaactcgca
     2461 ggcgagggtg gggtcatctc cccagaacca gggggtcgcg gtaccgccgc gtgcggcata
     2521 ctcccactgt gcctcgctgg gaaggccgta ccgttgcccg gtcttgcgcg acagccaccg
     2581 ggtgtagcca acggccatgt cgaagttcac gtagaggacg ggacgactgc cccggcccca
     2641 gccttcgtcg gccacggcct cgcaggcccc gtcggcgacg caggcatccc actggtcgta
     2701 ggtgatttcg tacttgccga tggcgaacgg ttcgacaaac gtcacctcgt gcagtggcgt
     2761 ctcgttggcc tcgtggccct gctcgtagtc ggccgagccc atcaggaagc tgccggctgg
     2821 cagcacgatc atctcggggc agtcggcaca gtccctgccg ccggtgatcg cgaacggggg
     2881 ggcgtccgcc ggatcggcgg cgcaggcgct gccggcgata ccggcggcca gcagcaggca
     2941 ggcgagtttc gatcgcctgc gccaggcagt cgcccgatct ggctgtccgc tgcgtgcggc
     3001 cgataccgga gaggccggga ttgtcttcat tggcgtgtcg tgcctcgatg ggggttgctg
     3061 gcgaagcgta cccgccgggg acaggccggg tgccaccggc cggttctggg ttcgccatcg
     3121 ccgaaaccgc atcgtgtcac ggcgtggcct catggacagg cgctcccggg tcgggcgtcc
     3181 cgggaggacg gtcgtcccag tgccacgggc gcggcagcgg gtgcggggtc ggccagcgtt
     3241 cgtgcagggt gtcgccgtcg aggaggcggc cgttcttcat cacctgcacg atcgacaggg
     3301 cgttggcaag gtcgctgcgt ggatccttcg ccagcaccac caggtcggcg aacttgccgg
     3361 cctccaagct gccgaactcc gcgtcgcggc cgatggtcgc ggcgccgccg agggtcgccg
     3421 cgagcagcac ctcggcgttg ctcatgccgc ccatcgcatg cgcctgcatt tcccagtgca
     3481 ggccgatgcc gggcatctcg ccgtgcgagc ccatgccgac gacgccgccg gcgcgcacga
     3541 cctcggcgac gcccgcggcg acgcgcggat acaggtattc gtccggctcg cgccaggtcc
     3601 gcgagcgcgt cttcatgtcg acgatgaagc ccggcgcgaa gcggcgcagc ttgcggtcgt
     3661 gggcaaggcc gccgcgcgag atgaagtcgt cctgcccctc cggcccgccg ttgccgatca
     3721 tcagcgtcgc cgagtacgcg gtgccggcgt cggcgaacag gcgcaccacg tcgcgctgca
     3781 gcggcaccgc gaccagcgcg tgctcgttgc cggcgtagcc gtcgagcgcg tgggtcaggc
     3841 ccagcttcat cgacagcgcg ccttcggtgg tcggcatcag gccggtttcg cgcgccgcca
     3901 tcgccaccca ctgccgcacg cggcggttgc cggtgcggta catcttgatg ttgccgaggc
     3961 gatagtgccc gcgatggcgc cgcagcacgt cgcggacctg ttcgtaggag gtgaactcgt
     4021 tgaacgagaa gatcgccggg ccggtggaga agatccgcgc cccgggcatc cgccccgctt
     4081 cgaccaggtc ctggtaggcc agggtgtcga tgctcagcgt ggacgggtcg aacaccgtgg
     4141 tcacgccgta ggcgaggttg gccagcggac cccagctttc gaggtcgagc acgtcgcggc
     4201 ggatgtcggc gatatgggtg tgggtgtcga tcaggccggg gatgaggtag cggccggaga
     4261 cgtcgaggac ggtggcgccg tccggcacgg ccacgctgcc ccgcttgccg acggcggcga
     4321 tgcggtcgtc gacgaccagc aggtcggcat cgacgacggc ctcgtcaccg cgcatcgtca
     4381 gcgccgttgc gccgcgcagc agcagcgcgc cgcgcggggt gtcgcgcggc acctcgacga
     4441 cggcggcgaa cgcctgcacg ccatcgcgac cgtcgaccgg catgtccacg gccatcgagg
     4501 gcgcgtcggc cgggtgcgtg cgcacgccgt ccagcgggcg ccggtaccag gtcgagccta
     4561 ccgcccagtg cagcgtgcgc ccgccgtcgc tccagccgaa gaagtcggcg ccgaccgtgg
     4621 tcagtttcgc gtgcgccacg cccggcgcgg caagatcgag cgtgccgccg gcgtcctccg
     4681 gcatcgccag caggtgcagc tgttgcgcga tctgcgccag cgcccagcgt ccgtcgggac
     4741 tgatgcgcag gtcgtcgacc ggcgcgcggc ccgcagcgaa gtaccagccg gggccggtga
     4801 cctgcagcac ggtgtcctgc ccgctgccgt cgagcgcgac gcgttcgagg ccgccgccgg
     4861 agagcacgtg cacgcgcgag gcatcgccat cgaagtgcgg ccggccgccc aggcgcccgc
     4921 tggcgaccac ccgcgcatcg ccgccgtcgc gctcgaacac caccagctcg ccgtcgcgca
     4981 gcgtgccgta ctccatgtag ctgtgcatgc gcacgctgtt gctggagcgg atcgcgacga
     5041 tgcgcgtgcc gtcgggcgtg aatacggggc gggtgtggaa ggcgggcgtg gagctgacct
     5101 gccggggctt cgagccagga gcgtcgagcg cgatgcgcca gacgtggccg gcgtccttcg
     5161 cggtccagtc gatgtaggcc agctcgcgac cgtccggcga ccacgacgga tggaaggccg
     5221 gccgcgtccc ggtgtcgatc cggcgcgccg tgccgctgcc gtccagcggc atcacgtaca
     5281 gcgcgcccag cgccgagaac gcgagccggg cgccgtcggg cgacggctcg ggggtctgga
     5341 tcaagcgcgc gcggaccggg ccggtgtcct gtcggatcga ctgccgcgtc gctgcgccca
     5401 gttcgatgtc gacgcgggcg cggaacggga tcgcctgtcg cgtgccgtcg gccagggcga
     5461 tgcgctgcag gccgccgtcg acgttgacga ccagggcgct gccgtcgggc gtgaacgcgt
     5521 gccgcggcag caggtcgcgc cagtgcgagg cgcccagttc gtcctgctgc accgggaacg
     5581 cgagccagcg gtcggcgcgg gtctgcaggt cgagcacgcg cagtccggtg cgggcctggt
     5641 ggcgggcgcc gtagaccagc cagcgcccgt cgggcgagac cgcagggcgc atcgcggtgc
     5701 ccagcaccag gtccggccgc gggctgcgcg gcgcggatac cagcgtctct tcgctgccgt
     5761 cgcgcaggtc gcggcgacgg atcgtccatt ccggcgtgcg cccggtctcg cgcgggccga
     5821 cgtgggcggc gtagtacacg aagcgcccgt cggccgacgg cgccgcaccc agcgccgagc
     5881 gccaggtggc gggcggctgg tcgtagcgct cgcggatcgg cagcagcagt tcgccgtcgc
     5941 cgtgcgcggc atcgaagcgc cagagctccc aggcctcgag ctcgggccgg tagcgcgaca
     6001 cgaagagcgc gcggccgtcg gcgctccacg ccggggaggc gaagatcggg ttgccgtcga
     6061 gcagggtgag ctggcgcggc gcgcggccgt cggcgtcgac cagccagagg ttctcggcgc
     6121 cggagcggtc gctgaggaag gcgatgcggc ggccgtcggg cgagaacacc ggctgcgaat
     6181 cgaaggccag gccgcgggtg atcgcgcggg ccgtgccgcc cgcggcgggc atcgtgtaga
     6241 ggtcaccgag cagttcgaac acgatgcggc ggccgtccgg cgacacgtcc ggcgacagcc
     6301 aggtgccttg gtcggtgtcg aaggcgatgc ggcgcccggg cgccatcggc agtgtctgcc
     6361 ccggctggcc gaccgcgtcg gcctgctcga aggcggggcc ctgcgacagc catggcggca
     6421 gcccggcatg gccgtgtcct tcgtgcgccc acgacgacag cggcagcagg agcgcgatcg
     6481 ccagcgcggg caggcgcggc cgttcgcgcg tcacggcgcg cgcgtccgcg cggggaggta
     6541 gcgatcgcgc agcagcgtct gccgcgtgcg cagcggaacg gcgtcgccgg ccacgaacac
     6601 cgcgaccggc gcggaagacg gctccagcgg atcgccgtcc cacagcacca ggtcggcgtc
     6661 cgcgccgggc gccagcgtgc cgatgcgcgc atctaggccc cagatacgcg ctggcgcttg
     6721 ggtgatcgcg gccagcgcgg cgtcgtcggg cagcccgtgc gcggcggcga tgccggccgc
     6781 gaggcgcagc gacgggcccg cgttccagct ggtgtagatg ccctgtgcgg aggcggtgaa
     6841 ggcgatgtcc accccggccc ggtgcagcag ggcggcgttg tcgtggcgcg cgccgagccg
     6901 gtcgtaggtc gctggcagcg cgtccagagg atcgagcacg acggcaatgc ccgcggcggc
     6961 gagctcgggc gccaggcgcc aggcctcggc cccgcccacg accaccacgc gcagcccgag
     7021 cgtgcgcgcc agcgcgatgg cttcgcggat gtcggcttcg cggtcggcga cgatggccag
     7081 cggcgtgcgc cgcatcgcca cttcgtgctg ggcgatgtcg tcggcatcga cgtggacgag
     7141 gccgtcggcg tggtgcggcg gcgtgcgccc gcgcccgagc ttcttgtgca gccgctgcca
     7201 cgcggcggcg tgcgagtgcg cgcgcgaggc gtcggtgccg atgtaggcga actgcaccac
     7261 gtcgccgctg cgctcgacga tgtcgccgcc ggtacgcagg tgcagcagcg tggcgcggcc
     7321 ggcgaagacg tcgtccgccg ccgccgccgg cgcgaccagg gcccggctga cgccgtcggc
     7381 gcgcgcctgt tcgaccgact gcgcgttggc gtcgatggcg tagtgggtcg tgtagcccgc
     7441 gcgcgatttg cccgaggcga cggcctcctg gccatcgccg acctcggaca ggccgatgcg
     7501 ggtggcggcg tggaacaggc ccggggtcag gtcgcggccg ccggcgtcga cgatgcgcgc
     7561 gccctgcggc gccgcgccgc cggcctcgac cgagacgatg cggccgtcgc gcagcaccac
     7621 ggtggcgtcc tcgaccaccg gcgccgacac cgggtgcgcg cgcgcgtgca cgatcgcgac
     7681 ggtgtccgcg gcccgcgcgg cgggcagcag gcaggccagc gcgaggacca gccagcgcat
     7741 cacggcccct gccccatcgc cgcgcggccg agttcgaaat cggacagcgg tcgcgcgccg
     7801 tcctcgacct gcaccacgcc gtcgatcatc accagccgcg gccggctgcg gacgctgaag
     7861 ggatcgccgc tccacagcac caggtcggcg ccgtagccgt gggcgatgcg gccgatgcga
     7921 tcacccaggc ccagcgcctg cgcagcgttg gaggtgatcc agcggatcgc gcgctcaggc
     7981 gggatatcga gcccggcgcg gcgcccggcg gcggcggcct tggccacttc gaggttgagc
     8041 aggctgccga gcaccgggat gtccgagtgc atgatcgcgc agccgccggc agcatcgacg
     8101 atggccgcgt tctcgcggat gccgtcctcg gcctcgcgct tgaaccccca ccagtcggac
     8161 cacaccgcgg cgcaggtgcc gtgttcgcgc agcaggccgg cgatcttgta ggcctcgctg
     8221 gcgtggtgga ccgcggcgat ccgcaggcca aactcgtcgg cgaccgccag cacggtggcg
     8281 atgtcgtcgg cgcggtagca gtgcacgtgc accggcatct cgccgcgcag cgccaacgcc
     8341 agcgtgtcct ggacaaggtc gggcaccgga tcgcgcccgc cggcacgcat ccgcgcacgc
     8401 gccgtgccca gcgccgcgcg cagttcgccg atcacgccca tgcggctgtc ggggaagccg
     8461 ggacggccat ccccggacag cgacgggttg ctgccacagg cgagcttgag gccctgcggc
     8521 gcgccgggga acttcatttc caccacggtc accgcccgca ccggcttgag caccacgccg
     8581 cggccggcga tcaccgacga cgagcccggc agcacctgca gcgtggtcac gccggcggcg
     8641 agtgcgtgct ggaaggcggg atcgctggcg cgcaccgcat gctcgatcca gatgtcgggc
     8701 gtccgcgaac ggctggtttc ggtgacgtcg gcgaaggcat ggcgaccgtg ttcgtagggc
     8761 tgtggaaacg tgcccaggtg tgtgtgcacg tcgatcagtc ccggcgttag ccaggcgccg
     8821 ccggcctcga ccacgcgcac gccgggttgc cgcggcaggt cgcgaccgat ctcggtgatc
     8881 cggccgcctt cgaccagtac gtcgtgtccg ttcaggcgcg cgccggcacc gtcgagcacg
     8941 gtggcgccgg cgaacagtgt cggcggcgcg tcggcgggaa cgtagcggct ggcgtaggga
     9001 tccgcgtcgg cggccgacgt tgccgacgcc gcgacaagga ggctcgcggc gaacagcgct
     9061 agggacagga acgctgggat gcgcacgcgg gccatgcgct cagccctcgc tcagccggtg
     9121 catccagtcc accagcaccg gatcccggtg tccggcaacg atctccgcga aagcctgcga
     9181 cgtctcgtcc tggccgagct tgaaggtcgc atgcgcgtgt tcgacgtgag cgcggaacgc
     9241 gatgatgtgt tcgcgcagct gcgcgtagcg cgcgcccatg cgttcggtcg tccacggctc
     9301 ggcttggccg gcctcgaggt gtgccgccaa ccgcgccagc gccgcgtcgg tctcctcggg
     9361 tgcaaactcg atgcgcacct gaaagcgcgc caccgcgtag ttccaggtcg ggccccagtc
     9421 cggcttcgac actagccgcg gcgcgacata ggcctgcggc ccttggaaca gtagcagcgc
     9481 ccgcggcgcg gcctgcagcg ccgcgcacag cgggttgcgg cgcgctaggt gcccgaacaa
     9541 cgacacgatc gcgccgccgg cgtcgcattc ggccaacagc ggcaacggcg ttgccggcgc
     9601 gtcgccctcg tgtgcgatca cccaggccag cgggaaggct tcgaccagcg ccaggacgtc
     9661 ggccggactg cgcggatcga acggcgtgct catggcgcca acagcgtccg ccgcagccag
     9721 tccgaggcct cgtcaagcgc gcggtcggcg agatcggaga tcgacatcgc ctcgaggaag
     9781 ctgtgggtgg tgccggcgta ggtgcgcgcc tgcacgggca cgccggcgtc gtgcagcgct
     9841 gcagccatct cgcggttgcc gtcggcgagg atgtcgcatt cggcaatcgc caggaacgcc
     9901 ggcggcagtc cccgcaggtc ggccagcagc ggcatcgcca gcggatcgtc gcggtcgccg
     9961 tcgccgcgca gatagttgtc ccagaagcgg tgcatctcgt cggcttccag catgtatgtt
    10021 gggccatcgt agcgcgccca cgacggcgac gtccggtcgc cataggcgcc gtagttgagc
    10081 agcatcgccg cgaccggcgg gcggccgagg tcgcgcagcc gcagctggct ggcgaccg
//
LOCUS       DAOMPG010000073        10128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2860_length_10128_cov_4.368212, whole genome shotgun sequence.
ACCESSION   DAOMPG010000073 DAOMPG010000000
VERSION     DAOMPG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_10128_cov_4.368212"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLS34_05610"
     CDS             complement(<1..324)
                     /locus_tag="PLS34_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86980.1"
                     /translation="MSVAALIVMTGFWPFGRAPDTDTATVGDAGPLVSEASAPPPPGD
                     PAAAAEAYAAFLASPAAADPAMRLTALRRLADLRLEAAELALMDGASPQARLDKLVGA
                     RFLDPI"
     gene            complement(321..2195)
                     /locus_tag="PLS34_05615"
     CDS             complement(321..2195)
                     /locus_tag="PLS34_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86981.1"
                     /translation="MRRSRLTLLVMLGAAMIAAPASAQSPGQAPAMVRDLAWGEVLFR
                     HYQQDRMGALVRLGMAEARGELPYHGAEARLLKGGLYLAWGLRDEAEVIFDELLAGGA
                     PPAQRAQAWYWLARIRHERGEHQRALDALARIDGQLPAAMAADRVDLEGRVLLALGRY
                     PDAARLLADSRQPGAWRAFADFNRAVALARSGRQEAALPLLESLGRARGGSEELATLR
                     DRANLALGLARLEAGDADGARAALDRVRLESPYAARALLAAGWAEAERERYREALGPW
                     STLAALQDVDGASLEAMLAVPWAWHQVDEPGESVLGYRRAAAACEAELGRLALAADAA
                     RGSELLAFVTADPEGDAAALDGPLPAYLHTLAVDHPFRAVAADLRDLHMLRENLLEWR
                     RSLDALRDMVATRRARFAQIAPRVELRLAGDELDHLESRYLAAEQRLEAVRSAADPSA
                     LASPDERRLLDRIATLEVRIAALPSGDARERMQERLRRLEGALAWQLNHEWPARIYQA
                     ERDLRAAGRQLEAARAGRASLATNLAEGPAGFEGYDVRIDAAATRVEHLLARVDADHA
                     KRFGELQALVLEELHDREQRVAAYLGQARFALAAAYDQAVQPRAARGDAALPGSGTTP
                     "
     gene            complement(2207..2719)
                     /locus_tag="PLS34_05620"
     CDS             complement(2207..2719)
                     /locus_tag="PLS34_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRP86982.1"
                     /translation="MRSPITALLVCLAMAAAPVAAEDAAPDEELRALKQELVALNRDL
                     FLLEEELLFPASTQVAVYLSLDVGKYFALDAVELSIDDQRVAKYLYTEREIDALHRGG
                     VHRLYVGNLKSGRHELVAVLTGRGPQGREYRRATSLEFDKGLGPQQLELVLRDSEALQ
                     QPTFQVKNWE"
     gene            complement(2727..3845)
                     /locus_tag="PLS34_05625"
     CDS             complement(2727..3845)
                     /locus_tag="PLS34_05625"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HRP86983.1"
                     /translation="MAATERQGRSRRHARWLARLAAALSCTCVAAFAATLPEDLAELM
                     YHSYDGGGVQVTGPALKFRKGFADDFSASATYYVDSITSASIDVVTSASPYTERRDEI
                     GLALDWLKGNSLMTVGYLASDENDYESGTWSIGVAQDFFGGMSTVTLGYMRGDDTVSR
                     VDTDFEDGIERNSYALGWSQVLTPKIVASLDYEAILETGFLNNPYRSARVLGAQVPEQ
                     YPRARNSHSVALRGIGMVSEATAVELGYRWFRDTWQVQAHTLEAALARRVRPGLTAEV
                     SLRYYAQDAASFYSDNFDAVYEFMARDKELSTFNSTSAGVRLTWDLGSTLGPLSRSSV
                     SFGYDFINFDYDDFTDIRNGELYGFDSHVFQVWFGGWW"
     gene            complement(3832..4053)
                     /locus_tag="PLS34_05630"
     CDS             complement(3832..4053)
                     /locus_tag="PLS34_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HRP86984.1"
                     /translation="MRRRTLLLVLLAALGGCAAPQPWVAPYEREALADPLMAFSRNPF
                     MDKHRQHVFDVREGARGATDGDGGGCGCN"
     gene            complement(4056..4511)
                     /locus_tag="PLS34_05635"
     CDS             complement(4056..4511)
                     /locus_tag="PLS34_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRP86985.1"
                     /translation="MVAANQSPVNVGAVAPDFALRSTAGGNLRLSEHRGQVVMINFWA
                     TWCGPCRQEMPKLDEIFARYEPAGFALLGVNIDEDAERAQRLADELGVSFPLLFDDEQ
                     SVSRLYDVQAMPMTVMVDRAGKVRSVHYGYRPGMEQRYLDEVRALLREE"
     gene            complement(4544..5203)
                     /locus_tag="PLS34_05640"
     CDS             complement(4544..5203)
                     /locus_tag="PLS34_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HRP86986.1"
                     /translation="MNSALRPFLLMATALAASGCSLLLGDPPADEPVEGPVPAVIDPM
                     LDRRDIVRPQIDTEDWEIGAFGGVLSVADFGAEAMYGLRFAYHPTEDIYLEGRYASSS
                     ISDTSFRRIGAPIFDVEKEDLTAYDLSVGVMVLPGEVFLGTRWARTSGVYMSFGVGRV
                     EFADRDSISYAFGFGLKVLPTDWLSLRLEARDTVFESDLLGENEWKHNFEINLGFGVF
                     F"
     gene            complement(5213..5992)
                     /locus_tag="PLS34_05645"
     CDS             complement(5213..5992)
                     /locus_tag="PLS34_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86987.1"
                     /translation="MSRRTDASVGWLRRLALCLAMLLGSLAAAAAGAAEDDALWLTVT
                     RPYAEMHSGPGRGYPVFHVVERGDSFTLVRQRTEWLMVETRDGVRGWMHINTVEASQG
                     PDGELVRFGRTGMDDWRGRRIELGFGFGDFEGDPLFSFRAGYRLGPHFVAEAQLLQAA
                     GSFSSTTAYHANLQVLPFLDGRIVPFFSLGAGKISNEPKSTLVDAAKVTDWAGNAGLG
                     VRGWLTRRFLLRGDVRHFVLTRDINNNDDFTEISVGFSVFF"
     gene            complement(5996..6493)
                     /locus_tag="PLS34_05650"
     CDS             complement(5996..6493)
                     /locus_tag="PLS34_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86988.1"
                     /translation="MLIFAVALLGACGGEVENTPVSSTGGTLCIADYTDCVNPIFDAI
                     INGRTGQTTCAASGCHDVGAGSGGAFKIFPGAAPDSVEMMANFFSARAFSNLDNPAQS
                     KLLLEPLQGVFSITGTHTGGDIFPDVNDPCYQAIRAWIAVRVEDEQAESCGFCTPPAL
                     AECGY"
     gene            complement(6525..9407)
                     /locus_tag="PLS34_05655"
     CDS             complement(6525..9407)
                     /locus_tag="PLS34_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86989.1"
                     /translation="MVRANRRKLELPALLALLMAIGGCGGGEETSSSAVVVEGDFAVV
                     FAKRQVSAVGNPTDGANFAPGGDLMMKDLSSPSAPLVNLTVDYTQGEGDVSDPEVSFD
                     GERVLFSMRGPGNPTWNVFELDLPTRTLRRIIVDDAVANAGDDVDPAYLPDGRIVFSS
                     NRQEKSRQLLAERNIEPYAYRDEYERERVIALHVMSSEGSDIRQISFNQSHDRNPTVL
                     NTGEILFARWDHVGNRNHFPLFITNPDGTNIFVQYGAFSPGNSFLHPREMPDGRVMSS
                     LMPLSGTREGGALMAVDIRNFAESGERVPGAPADAVAQEQLTLHPIEFGMNVSPFGRY
                     TTPYPLWDGTNRALVSWSPFRPEMEFNPVSGQDEEVEGPPLYGVYMFNLDTRTLRPIA
                     LPQEGWAYTDPIAVQARPVPNTIPDKPLDAALAAEGMGVLNVKSVYDTDGLGLMGAGM
                     LVAGESIPMTTPPPDDTRASVADLMTLKDPALTTAADRPARFVRVTAAVPTPPGLSRE
                     AIGETDLEMQQIVGYAEIEPDGSFRIKVPADTPLGVVAVDSEGRAIQVHTNWLQVRPG
                     ETRTCNGCHSPRRGSALNSAPIAGFHPNATMPGELGESMAETRTRLNPAAMELSFDLV
                     YEDVWTDPAQAGRAPDAPLLISYANLPPTVPAPTEGIVNYPDHIQPLWDADRGASTCV
                     DCHASDDPDDPVSAGLDLRGTTSGSGRLKSYEEIMVGDPVIDEATGLPQLRIRENGEI
                     EIVRTAPLVSTGDSRDSSRSSALMAKLYEAPLRSGRDMSEPPMVDHSGMLNASELRLL
                     AEWIDLGGQYYNDPFDDANGDGFRALSEVRGGLQGLSEPLFAATVHPVLMSQCAACHQ
                     PFGNTGTPSTPNEPVNPGFRTSRYVLTGNVEGDFNVSVSMVADVCDPESSYLLLRPSS
                     TELDDPPHPRIGVDPEDPDSGTPVLLPGSAGWQAIYDWIAAGDCEV"
     regulatory      complement(9556..9644)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9917..>10128
                     /locus_tag="PLS34_05660"
     CDS             9917..>10128
                     /locus_tag="PLS34_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRP86990.1"
                     /translation="MGFWILIAVVAAVVLWAVATYNKLVNLRNAVKNAFAQIDVQLTR
                     RYDLIPNLVEAVKGYMKHERETLEAV"
BASE COUNT         1570 a         3541 c         3480 g         1537 t
ORIGIN      
        1 gatcggatcg aggaacctgg cgccaacgag cttgtcgagg cgcgcctgcg gcgacgcgcc
       61 gtccatcagc gcgagctccg ccgcctccag gcgcaggtcg gcgagccgtc gcagggcggt
      121 gaggcgcatg gcagggtctg cggcggcggg cgatgcgaga aacgctgcgt aggcctcggc
      181 cgcggctgcc gggtcacccg gcggcggcgg cgcactcgcc tcggagacca gcggccctgc
      241 atcgcccacc gtcgcagtgt ccgtgtccgg cgcacgcccg aacggccaga aacccgtcat
      301 cacgatgagc gcggcgacgc tcatggcgtc gtccccgagc ccggcagggc ggcgtcgcca
      361 cgggccgcgc gtggctgcac ggcctggtcg taggcggctg ccagcgcaaa gcgggcctgg
      421 ccgaggtagg cggcgacgcg ctgctcacga tcatggagtt cttccagcac cagcgcctgc
      481 agctcgccga agcgcttcgc atggtccgcg tcgacacgcg ccagcaggtg ctccacgcgc
      541 gtcgcggcag cgtcgatgcg gacgtcatag ccctcgaaac ccgccggccc ctcggccagg
      601 ttcgtggcca ggctggcgcg gcccgcacgg gccgcctcga gctggcgccc ggccgcgcgc
      661 aggtcacgct ccgcctggta aatgcgcgcc ggccattcat ggttgagctg ccaggccagc
      721 gccccttcca gccggcgcag ccgctcctgc atgcgctcgc gcgcatcgcc cgatggcaac
      781 gcagcgatgc ggacctcgag cgtggcgatc ctgtccagca ggcgccgctc gtccgggctg
      841 gccagcgcgc tcgggtcggc cgcggagcgc accgcctcga gccgctgctc ggccgccagg
      901 tagcgcgatt cgaggtgatc cagttcgtcg cccgcgaggc gcagctcgac ccgcggcgca
      961 atctgcgcga agcgtgcgcg acgcgttgcc accatgtccc gcagggcgtc gaggctgcgg
     1021 cgccattcca gcaggttctc gcgcagcatg tgcaggtcac gcaggtcggc ggcgaccgcg
     1081 cggaaggggt gatccacggc aagggtatgc aggtacgccg gcagcggccc gtcgagcgcc
     1141 gcggcatcac cctccgggtc ggccgtcacg aaggccagca gttcgctgcc gcgcgccgca
     1201 tcggccgcca gcgcgagccg gccgagctcg gcctcgcagg cggcggccgc gcgtcgatag
     1261 ccgagcacgc tctcgccggg ttcgtcgacc tggtgccatg cccagggcac ggccagcatc
     1321 gcttcgagcg acgcgccatc cacgtcctgc agcgccgcca gcgtgctcca cgggccaagc
     1381 gcctcgcggt acctttcacg ctccgcctcg gcccaaccgg ccgccagcag ggcacgcgcc
     1441 gcataggggc tctccagccg cacccggtcg agcgctgcgc gcgcgccatc agcgtcgccg
     1501 gcctcgaggc gcgccagccc gagcgccagg ttggcgcgat cccgcaaagt ggccagctct
     1561 tcactgccgc cgcgggcacg acccagcgac tccagcaacg gcagcgcggc ctcttgccgg
     1621 ccggagcgcg ccagcgcgac ggcgcgattg aaatcggcga aggcgcgcca ggcgccgggc
     1681 tgacggctgt ccgccagcag ccgcgccgcg tccgggtaac ggcccagcgc cagcagcacc
     1741 ctgccctcga gatccacgcg gtcggccgcc atggccgcgg gcagctgtcc gtcgatccgt
     1801 gccagggcgt ccagggcgcg ctggtgctcg ccccgctcat gccggatgcg cgccagccag
     1861 taccaggcct gcgcgcgttg cgccggcgga gccccgccgg ccagcagctc atcgaaaatc
     1921 acctcggcct cgtcgcgcag gccccaggca aggtacagcc cgcccttcaa caggcgcgcc
     1981 tcggcgccgt ggtagggcaa ctcgccgcgc gcctcggcca tgccgagccg caccagcgcg
     2041 cccatgcggt cctgctggta gtgccggaac agcacctcgc cccaggccag gtcgcgcacc
     2101 atcgcgggcg cctggccggg cgactgcgca gacgcaggcg cggcgatcat cgccgcgccg
     2161 agcatcacca gcagtgtcag ccgagaacgc cgcatggttc agcccctcac tcccagttct
     2221 tgacctggaa agtcggctgc tgcagggctt cgctgtcgcg caacaccagc tcgagctgct
     2281 ggggaccgag gcccttgtcg aactcgaggc tggtggccct gcggtactcg cgaccctgcg
     2341 gcccgcgtcc cgtcagcacc gcgaccagct cgtggcgccc gctcttgagg ttgccgacgt
     2401 agagccgatg caccccaccc cggtgcaggg cgtcgatctc ccgctcggtg tacaggtact
     2461 tggcgacccg ctggtcgtcg atcgacaact ccacggcatc gagcgcgaag tacttgccga
     2521 cgtccagcga caggtacacg gcgacctggg tgctggccgg gaacagcagc tcctcttcca
     2581 gcaggaacag gtcgcgattc agcgcgacca gctcctgctt gagggcgcgc aactcctcgt
     2641 ccggcgcggc gtcctccgcg gcgaccggcg ccgccgccat ggccaggcat acgagcagtg
     2701 cagttatcgg gctgcgcatc gcggacctac caccagcccc cgaaccagac ctggaaaacg
     2761 tggctgtcga atccatagag ttcaccgttg cgtatgtccg tgaagtcgtc gtagtcgaaa
     2821 ttgatgaagt cgtagccgaa gctcaccgaa ctgcggctga gcggaccgag tgtgctgccg
     2881 aggtcccagg tgaggcgcac gcccgcggag gtgctgttga aggtcgacag ctccttgtcg
     2941 cgcgccatga attcgtacac ggcgtcgaag ttgtccgagt agaacgatgc ggcgtcctgg
     3001 gcgtagtaac gcagcgaaac ttccgccgtc agccccggac gaacacgccg cgccagggcg
     3061 gcttccaggg tgtgcgcctg cacctgccag gtatcgcgga accagcggta acccagctcc
     3121 actgcggtgg cctccgacac catgccgatg ccgcgcagcg ccaccgagtg gctgttgcgg
     3181 gcgcgcgggt attgttcggg cacctgggcg ccgagcacgc gcgccgagcg ataggggttg
     3241 ttcaggaagc cggtttcgag aatggcctcg taatcgagac tggcgacgat cttcggcgtc
     3301 agcacctggg accagccgag cgcgtagctg ttgcgctcga tgccgtcctc gaagtccgtg
     3361 tcgacgcgcg acaccgtgtc gtcgccgcgc atatagccga gcgtgacggt gctcatcccg
     3421 ccgaaaaagt cctgcgccac gccgatggac caggtgccgg actcgtagtc gttctcgtcg
     3481 ctggccaggt agccgacggt catcagcgaa ttgcccttca gccagtccag cgccaggccg
     3541 atctcgtcgc gccgctcggt gtacgggctg gcgctcgtga cgacgtcgat cgaggccgaa
     3601 gtgatgctgt ccacgtagta ggtggcggag gccgagaaat cgtctgcaaa gcccttgcgg
     3661 aacttcagcg cgggtccggt gacctgcacg ccgccgccgt cgtagctgtg gtacatgagt
     3721 tcggcgagat cctccggcaa cgtggccgcg aaggccgcga cacaggtgca cgacagcgcg
     3781 gccgccagcc gcgccagcca ccgcgcatgc cgccgggacc ggccctgccg ctcagttgca
     3841 gccacacccg cctccatcgc cgtcggtcgc cccgcgcgcg ccctcgcgca cgtcgaacac
     3901 gtgctggcgg tgcttgtcca tgaagggatt gcgcgagaaa gccatcaacg ggtccgccag
     3961 cgcctcgcgc tcgtagggcg cgacccacgg ctgcggcgca gcgcagccgc cgagggccgc
     4021 gagcaggacc agcaacagtg tgcggcgccg catgctcatt cctcccgcaa caacgcccgc
     4081 acctcgtcga ggtagcgctg ctccatgccg gggcgatagc cgtagtgcac gctgcggacc
     4141 ttgccggcgc ggtccaccat cacggtcatc ggcatcgcct gcacgtcgta caggcggctg
     4201 acgctctgct cgtcgtcgaa cagcaaggga aagctgacgc cgagctcgtc cgccagccgc
     4261 tgcgcgcgct cggcgtcctc gtcgatgttg acgccgagca gggcaaagcc ggccggctcg
     4321 tagcgggcga agatctcgtc cagcttgggc atctcctgcc ggcacgggcc gcaccaggtc
     4381 gcccagaagt tgatcatcac cacctggccg cgatgctcgg acaggcgcag gttgcccccc
     4441 gccgtgctgc gcagcgcgaa gtccggcgcg acggcgccga cgttcaccgg cgactggttg
     4501 gccgcgacca tcgccggcac gccgagtgcc agcgcggcac cgatcagaaa aatacgccga
     4561 agccgagatt gatttcgaaa ttgtgcttcc actcgttctc tccgaggagg tctgattcaa
     4621 agacggtatc gcgggcctcg aggcgcaggc tcaaccagtc ggtgggcagc accttgagtc
     4681 cgaagccgaa ggcgtaactg atgctgtcgc ggtcggcgaa ctccacccgg ccaacgccga
     4741 agctcatgta cacgccgctg gtgcgcgccc agcgcgtgcc gagaaacacc tcgcccggca
     4801 ataccatgac cccgaccgac aggtcgtagg ctgtgaggtc ttccttctcg acgtcgaaga
     4861 tcggcgcgcc gatgcggcgg aagctggtgt cgctgatcga ggagctcgca tagcgcccct
     4921 ccaggtagat atcctcggtg gggtggtacg cgaagcgcag gccgtacatg gcttcagcgc
     4981 cgaagtctgc caccgagagc acgccgccga aggcgccgat ctcccagtcc tcggtgtcga
     5041 tctgcggccg cacgatgtcg cgacggtcca gcatcgggtc gatcaccgcc ggcaccgggc
     5101 cctccaccgg ctcgtcggcc ggcgggtcgc cgagcagcag gctgcaaccg ctcgccgcca
     5161 gggcggttgc catcaacagg aacgggcgca gtgcggaatt catctgtcgt cctcagaaga
     5221 acaccgagaa gcccacggaa atctcggtga agtcgtcgtt gttgttgatg tcgcgcgtca
     5281 gcacgaagtg gcgcacgtca ccccgcaaca ggaagcgccg ggtcagccag ccgcggactc
     5341 cgagcccggc atttcctgcc cagtcagtta ctttggcggc atcgacgagc gtacttttag
     5401 gctcgttgct gatctttccc gcgccgaggc tgaaaaaagg cacgattcga ccgtccagga
     5461 acggcagcac ctgcaggttg gcgtggtacg cggtggtgct ggagaagctg ccggcggcct
     5521 gcagcagctg ggcctcggcg acgaagtgcg ggcccaggcg atagccggcc cggaagctga
     5581 acagcgggtc gccctcgaaa tcgccgaagc cgaagccgag ctcgatgcgc cggccgcgcc
     5641 agtcgtccat gcccgtccgg ccgaagcgca ccagttcccc gtcgggaccc tggctggcct
     5701 ccacggtgtt gatatgcatc cagccgcgca cgccgtcgcg cgtttccacc atcagccact
     5761 cggtgcgctg gcggaccagc gtgaacgagt cgccccgctc gacgacatgg aacacggggt
     5821 agccgcgtcc cgggcccgag tgcatctcgg cgtatggccg ggtgacggtc agccagagcg
     5881 cgtcatcctc ggcagcgcct gcggcagctg ccgccaggct gcccagcaac atcgccaggc
     5941 aaagcgccag tctgcgcagc caaccgacag atgcgtccgt gcggcggctc atgcctcaat
     6001 acccgcattc ggccagcgcc ggcggcgtgc agaagccgca gctctcggcc tgctcgtcct
     6061 ccacgcgcac cgcgatccac gcgcggatgg cctggtagca gggatcgttg acgtccggga
     6121 aaatgtcgcc gccggtgtgc gtgccggtga tcgagaacac gccctgcaat ggctcgagca
     6181 gcagcttgct ctgggcgggg ttgtcgaggt tggaaaacgc ccgcgcggag aagaagttgg
     6241 ccatcatctc gacggagtcc ggcgccgccc cggggaagat cttgaacgcg ccgcccgagc
     6301 cggcgccgac gtcgtgacag ccgctggccg cgcaggtcgt ctgcccggtg cggccgttga
     6361 tgatggcgtc gaagatcggg ttgacgcagt cggtgtagtc cgcgatgcac agggtgccgc
     6421 ccgtcgagga caccggcgtg ttctcgacct cgcccccgca ggcgcccagc agggcgacgg
     6481 cgaagatgag caatggacgg caccccgtgt tgcgcatggc ggcgctagac ctcgcagtcg
     6541 ccggcggcga tccagtcgta aatggcctgc catccggcac tgcccggcag gagcaccggg
     6601 gtgcccgaat ccgggtcttc cggatccacg ccgatgcgcg gatgcggcgg atcgtccagc
     6661 tcggtcgagc tgggccgcag cagcaggtag ctcgactccg ggtcgcacac atcggcgacc
     6721 atcgacacgg acacgttgaa gtcgccttcc acgttgccgg tcaacacgta gcgcgaggtg
     6781 cggaatcccg ggttgacggg ctcattcggc gtcgacggcg tgccggtgtt gccgaagggc
     6841 tggtgacagg cggcgcactg cgacatcagc accgggtgca cggtcgcggc aaacagcggc
     6901 tcggaaagcc cctgcaggcc accgcgcact tcggacaggg cgcggaagcc gtcgccgttg
     6961 gcgtcgtcga agggatcgtt gtagtactgc ccgccgaggt cgatccactc cgccagcaag
     7021 cgcagctcgc tggcgttcag catgccgctg tggtccacca tcggcggttc gctcatgtcg
     7081 cgcccggagc gcaacggcgc ctcgtagagc ttggccatca gcgcgctgga gcgcgagctg
     7141 tcgcgcgagt cgccggtgct gaccagcggc gcggtgcgca cgatctcgat ctcgccgttt
     7201 tcgcggatgc gcagctgcgg caggccggta gcctcgtcga ttaccgggtc gccgaccatg
     7261 atctcctcgt aggacttcag ccggccggag ccggaggtcg taccgcgcag gtccaggccg
     7321 gccgacaccg ggtcgtcggg atcatcgctg gcatggcagt ccacgcaggt ggatgcaccg
     7381 cggtcggcgt cccacagggg ctggatgtgg tcgggatagt tgacgatgcc ctcggtgggc
     7441 gccggcaccg tcggcggcag gttcgcgtag ctgatgagca gcggcgcgtc gggcgcacgg
     7501 ccggcctgcg ccgggtcggt ccagacgtcc tcgtacacca ggtcgaagct cagttccatg
     7561 gccgccgggt tcaggcgcgt gcgcgtctcg gccatcgact cgccgagctc gcccggcatg
     7621 gtggcgttgg gatggaagcc ggcgatgggc gccgagttca gcgcgctgcc gcgccgcggc
     7681 gaatggcagc cgttgcaggt ccgggtctcg cccgggcgca cctgcagcca gttggtatgc
     7741 acctggatgg cacgaccttc cgagtccacc gcgaccacgc cgagcggcgt gtcggccggc
     7801 accttgatac ggaaggagcc gtcgggctcg atctccgcgt aaccgacgat ctgctgcatc
     7861 tcgaggtcgg tctcgccgat cgcctcgcgc gacaggcccg gcggcgtggg cacggccgcg
     7921 gtgacgcgca cgaagcgcgc cggccggtcg gccgcagtgg tcagcgccgg atccttcagc
     7981 gtcatcaggt cggccaccga ggcgcgggtg tcgtcgggcg gcggcgtcgt catggggatg
     8041 ctctcgccgg ccaccagcat gccggcgccc atcaggccca gcccgtcggt gtcatagacg
     8101 ctcttgacgt tcagcacgcc catgccctcg gcggcgagtg ccgcatcgag gggcttgtcg
     8161 ggaatggtgt tcggtaccgg ccgcgcctgc accgcgatgg gatccgtata ggcccagcct
     8221 tcctgcggca gggcgatggg gcgcagggtc ctggtgtcca ggttgaacat gtacacgccg
     8281 tacagcggcg gcccctcgac ctcttcgtcc tggccgctca ccgggttgaa ctccatttcg
     8341 gggcggaacg gcgaccagct caccaacgcg cggttggtgc cgtcccagag cggatagggc
     8401 gtggtgtagc ggccgaacgg cgacacgttc ataccgaact cgatcgggtg cagcgtcagc
     8461 tgctcctgcg cgaccgcgtc ggccggagcg ccggggaccc gttccccgga ctcggcgaaa
     8521 ttgcggatgt ccacggccat cagcgcgccg ccctcgcggg tgcccgacag gggcatcagc
     8581 gaactcatta cgcggccgtc gggcatctcg cgcgggtgca ggaagctgtt gccggggctg
     8641 aaagcgccgt actgcacgaa gatgttggtg ccgtcgggat tggtgatgaa cagcgggaaa
     8701 tggttgcggt tgccgacatg gtcccagcgc gcaaacagga tctcgccggt gttcagcacc
     8761 gtcgggttgc ggtcgtggct ctggttgaag gaaatctgcc ggatgtcgct gccctcggag
     8821 ctcatcacgt gcagcgcaat gacgcgctcg cgctcgtact cgtcgcggta ggcatagggc
     8881 tcgatgttgc gctcggccag cagctggcgg gatttctcct gccggttcga gctgaacacg
     8941 atgcgcccgt ccggcaggta cgccgggtcg acgtcgtcgc ccgcgttggc gacggcatcg
     9001 tccacgatga tgcgccgcag ggtgcgggtc ggcaggtcga gctcgaagac gttccaggtc
     9061 gggttgcccg gcccgcgcat ggaaaacagc acgcgctcgc cgtcgaagga cacctcggga
     9121 tccgagacgt cgccttcgcc ctgggtgtag tccaccgtga ggttgaccag cggcgcgctc
     9181 ggtgaggaca ggtccttcat catcaggtca ccgcccggcg cgaaattcgc gccgtcggtg
     9241 ggattgccca ccgccgagac ctggcgcttg gcgaacacca cggcgaagtc accctcgacc
     9301 accaccgcgg acgaactcgt ctcctcgccc ccgccgcatc cgccgattgc catgagcagc
     9361 gccagcagcg ccggaagctc cagctttcgt ctatttgccc gaaccatgtc ccgacctccc
     9421 gttggagtat cgtcggcagg tgggggcgca aacctgaggg tcccgggtcg atcgacccgg
     9481 tgcagcggcg ggttgtacag ctgggaggac ccgcagaacg tgcgggcggg cggatggccc
     9541 gatgacttct ccctggccgg caaccccgct gtcatgggtc cgggaggacc tttagaccgg
     9601 tggctttgcg tccccgcctt tcgacgggtt tgcctttgtc agcggcgcca cggatgccag
     9661 aggcgcctgg gactgtcaag caggcagtcg gcattgcggc gacgcatcaa ttcccgcagt
     9721 atcggcctgg acacgcttgg aacgattcca agctgcggtt tttacttagt attcctcccg
     9781 ggcgcccgac cctgccgccg ccggcgccgg gccggcctcg tcacgggggc ggcgacgccg
     9841 ccaatcgcgc ggcggcaggt gaccctgcgc gtcacctcgc gtattctccg gtcgcatcat
     9901 ccggggagga atcgtcatgg gcttctggat tctgatcgct gtcgtggccg ccgtcgtcct
     9961 gtgggccgtc gcgacctaca acaagctcgt caacctgcgc aatgcggtca agaacgcgtt
    10021 cgcccagatc gacgtccagc tgacccgccg ctacgacctg atcccgaacc tggtcgaggc
    10081 ggtcaagggc tacatgaagc acgagcgcga aacgctcgag gcggtcat
//
LOCUS       DAOMPG010000074        10111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2868_length_10111_cov_4.264519, whole genome shotgun sequence.
ACCESSION   DAOMPG010000074 DAOMPG010000000
VERSION     DAOMPG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_10111_cov_4.264519"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1102)
                     /locus_tag="PLS34_05665"
     CDS             complement(26..1102)
                     /locus_tag="PLS34_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP86991.1"
                     /translation="MQRIITTIFVLAAAGLAAWIWFGTSDNGGTPQTVPVEDRPPVAV
                     VMTEAVERLLVDEVEALGTTRAREAVEIAPRIASVVTAIRFREGEMVEAGAVLVELDN
                     AEERASLAEAEATVIDSRAQFRRARELLASKTVSESQVQQLEATMNADEARLRAAQAR
                     FEQTLVRAPFTGRVGLRQVSPGSLVSPGTVITTLDDLSSVRLDFTVPESFLGVLQSGL
                     EIRARSVAFEGRAFDGTVSTINTRVDPVTRAVTIRAELPNDDGLLKPGMFLTVRLAGQ
                     ARARVVVPEAALVPEGDRQMVYTVRDGRSWRTEVVVGRRLRGEVEILEGLQAGDSVVI
                     EGTQKVANGGRVTPAAPIATLGRG"
     gene            complement(1359..1826)
                     /gene="bfr"
                     /locus_tag="PLS34_05670"
     CDS             complement(1359..1826)
                     /gene="bfr"
                     /locus_tag="PLS34_05670"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005526263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRP86992.1"
                     /translation="MKGDAKVIEHLNKILKNELTAINQYFLHSRMLKDWGLEKLADKE
                     FHESVDEMKHADELTQRILFLEGLPNLQDLGKLMIGENVKEILECDLKMELAGLKDLK
                     NAISHCESSGDYVSRHLFREILSSEEEHVDWLETQLGLIDKMGLENYVQSQAS"
     gene            complement(2010..2225)
                     /locus_tag="PLS34_05675"
     CDS             complement(2010..2225)
                     /locus_tag="PLS34_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRP86993.1"
                     /translation="MYVCICKRVTDHQIHDAIGRGARSIEDLGRELGVATGCGQCACF
                     ASEMLERAPAASGAMTGVRQSVHPDAA"
     gene            complement(2757..5048)
                     /locus_tag="PLS34_05680"
     CDS             complement(2757..5048)
                     /locus_tag="PLS34_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP86994.1"
                     /translation="MRLPRPAHPLALLSIAAFAGPALADDATLVVAQADATDETETTT
                     PLVLSAIPPLDPAQLGSVTIVGSAAAARDIGGSAHFIGPDELEEFKYSDIHRVLRSVP
                     GVYLQDEEGFGHRPNIGIRGSGQDRSSRIAVLEDGVLIAPAPYSAPAAYYFPNTRRVY
                     AAEVLKGPASIPVGPRTIGGALNVLSTPIPNEWGMYADYFYGQNDTHDARIIAGGSGD
                     HFGLLFETVQQQSDGFKTIDPNDRNTTAGYDFDDILLKARVNTDPGARIYQALEFKYG
                     RAEQDAQQSYLGLTDEDYAADPFRLYAATQIDQLDAEFKLRQVSWLIEPAAMPWKASL
                     TYYDNEFARSWYRVNNVGGIGLSAILEDPGNYADQLSWLKGATSPDDALRARDNYRTY
                     GSKGLQGRGEYDIDAGAAAVKLKAGFRFHEDFEDRFQKEDRYRMEDNNLVLTTAGIAG
                     GQENRVGEADVSSVFIAADIDVGALRVSPGVRYEHIRLKRSDYSRTVPDRSEGPTQVR
                     QSSATEWITGIGATYALTDRVKLLGGVHEGFNPSSPGSTAGAETSTNWEFGVRYEGAR
                     YYAEAIGFITDYGNLVGTVTESTGGGGEIGDQFEAGEAVVRGIELLGETVLWEFAGGW
                     RVPARLAWTWTPDAEFDNSFASSFAPWGEVEAGDRMPYLPEHVGQIRVGLEGDRLELN
                     LNFAYQSETRTVAGTGAIPANERTDSAFVVDLAASYRLTRHFSLLARVQNLFDEEYIA
                     SRSPNGVRPGHDRLAMVGVQASF"
     gene            5422..6750
                     /locus_tag="PLS34_05685"
     CDS             5422..6750
                     /locus_tag="PLS34_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRP86995.1"
                     /translation="MLPPRDRLRRILLLALPIIGGMVSQNVMNLVDTAMVGTLGDAAL
                     AAVGLGGFANFMFMALLLGLSVGVQTTASRRKGEGKLDETAIPLNGGLFIVLCIAPPL
                     SALLYWAVPHIYPHLNSDPDVIAQGVPYLQARVLAMLFMGANFAFRGYWNAVDLARLY
                     MNTLIAMHACNIFLNWVLIFGNLGAPALGTQGAGIASALATVVGCGLYIWLGFRHARS
                     AGFMQRIPARHNLLALFRLSLPTGIQQLFFSTGFVLTFWIIGQVGTREMAAATVILNL
                     TLVALLPGLGLGLAAATLVGQALGRSDVNDASRWGWDVAKVGMLGLLLLGLPGAIWPE
                     LLLGVFIHDPDTMEVARFPMRLVGLGMAIEGLGVVMMHALLGAGDTQRVMKVAVLAQW
                     AAFLPLAYLAGPVLGFGLTFIWCMQVGYRAAMAGVFTLFWVRRGWARIKV"
     gene            complement(7017..7310)
                     /locus_tag="PLS34_05690"
     CDS             complement(7017..7310)
                     /locus_tag="PLS34_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRP86996.1"
                     /translation="MTRDEMDILMGQLLDESLELEFEEFCRICHASEEFVTALVAEGV
                     IEPRGEQRGQWRFTGRSVRRTQVAVRLHHDLDVNLPGVALALDLLEEIERLRR"
     gene            complement(7315..8283)
                     /locus_tag="PLS34_05695"
     CDS             complement(7315..8283)
                     /locus_tag="PLS34_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRP86997.1"
                     /translation="MDFKDYYATLGVERGASGEEIKRAYRKLARKYHPDVSKEADAEA
                     RFKEVGEAYEVLKDPEKRAAYDQLGADWKAGQEFRPPPGGGAREYYYSSGGGPGGEQF
                     GGFSEFFESLFGRGGMAGGARGGPGAGPAGGAFHYRQAGPMRGEDQSARVKISLEDAF
                     HGANRTLEVRSPGKGKHNIRVAIPKGVTEGQRIRLAGQGTPGANGGPAGDLFLEVMFE
                     PHPIFEAQGRNILLTLPVAPWEAALGETVGVPTLGGKVELRIPAGSQSGRRLRLKGRG
                     LPGTPPGDQIVTLRIDTPPADTSFARTLYEEMRKKMPFNPRRDIEG"
     gene            complement(8522..8872)
                     /locus_tag="PLS34_05700"
     CDS             complement(8522..8872)
                     /locus_tag="PLS34_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86998.1"
                     /translation="MAADPADRALMLGTGVGADEWGIGTSDLDIPHRVQLQAAWQAAP
                     PAGEHQQRERAERRRRQPVPHGSDQHRDNLVLDCVRRIVARSPPGSVTLFSVPGKNPD
                     TACRFFLRFRVGPS"
     gene            8871..>10111
                     /locus_tag="PLS34_05705"
     CDS             8871..>10111
                     /locus_tag="PLS34_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP86999.1"
                     /translation="MTAAWAAAAAFTPETRAHWQLVGLLEIASNTAWLVFLARLSPAG
                     QGLDGARRQFIAHAPAAFVFLYGTIIIAGARGDATIPVLTQALIITGLVTAIGGLVLL
                     EQAFRAMRAAPRQQILPLIIGLGGILAYDLFLYSHGMLFLGLHEELWGARGFANLMAV
                     PFLLLAARRNPDWSVDVFVSRHVAFYTTSLVGIGAYLLVMAVAGYGLRVAGGEWGFIF
                     QVIFFFGALVVLAWVLFSPSARAGLRVFLAKHFYSNKYDYREEWLKLTQRLADASGRE
                     SPAVRGLGALVDMVGADGAILWERTGQEPAAPSDADPDEPPIDDIASFQRTATTGSVG
                     AQATLPRTLQAADPVATFLGERRWTINLSQARRNPDSHPGLALPPWTDQLGTEALVVP
                     LLNRDRLQAIAALLNPSRVGRL"
BASE COUNT         1533 a         3345 c         3417 g         1816 t
ORIGIN      
        1 cagctcggag attttcatgg gtgcgtcagc cgcggccgag cgtcgcgatc ggcgcggcgg
       61 gggtgacgcg tccgccgttg gcgaccttct gcgtgccctc gatcaccacg ctgtcgccgg
      121 cctgcaggcc ctcgaggatc tcgacctcgc cgcgcagtcg acggccgacc accacctcgg
      181 tgcgccagga gcggccgtcg cgcaccgtgt acaccatctg tcggtcgccc tccggcacca
      241 gcgcggcttc cggcacgacc acgcgggcgc gcgcctggcc ggcgaggcgc acggtgagga
      301 acatgcccgg cttcaacagg ccgtcgtcgt tcggcagctc ggcgcggatg gtcaccgcgc
      361 gcgtcacggg gtcgacgcgg gtattgatgg tggagacggt gccgtcgaag gcgcggccct
      421 cgaaggccac gctgcgcgcc cggatctcga ggccggattg cagcacgccg aggaaggact
      481 cgggcacggt gaaatcgagg cgcacgctgg acaggtcgtc gagcgtggtg atgacggtgc
      541 cggggctgac caggctgccg gggctgacct ggcggaggcc gacgcgaccg gtgaacggcg
      601 cgcgcaccag ggtctgctcg aatctcgcct gggccgcgcg caggcgggct tcgtcggcgt
      661 tcatggtggc ctcgagctgc tgcacctgcg actcggacac ggtcttcgag gcgagcagtt
      721 ccctggcgcg gcgaaactgt gcgcggctgt cgatgacggt ggcctcggcc tcggccagcg
      781 aggcgcgttc ctcggcgttg tcgagttcca ccagcaccgc gccggcctcg accatctcgc
      841 cctcgcggaa gcggatggcg gtgacgacgc tggcgatgcg cggcgcaatc tcaacggcct
      901 cgcgggcgcg ggtggtgccc agcgcttcca cctcgtcgac cagcaagcgc tcgacggctt
      961 cggtcatgac cacggccacc ggcgggcgat cctcgaccgg cacggtctgc ggggtgccgc
     1021 cgttgtcgct cgtgccgaac cagatccagg ccgccagccc ggcggccgcc agcacgaaga
     1081 tagtggtgat gattctctgc aatctaggaa ctcgcgcgat ggccggcgcg ctcggccgcg
     1141 cctgctatga ctgaccctgc cgggccggga tagttcgtgc tcgaattgct gcgattctag
     1201 cgcggcgggc cgcgccgacg caccggtgaa gtatggattc ggcgtggaat cgcaatcgga
     1261 tgtgaaagcg tggtcagaaa acggggtcag accccggttc tgaaatcggg gtcagacccc
     1321 ggtttctgga aatcggtgtc tgaccccggt tgcggaggtc aagacgcctg cgactggacg
     1381 tagttctcga ggcccatctt gtcgatcagg ccgagttggg tctcgagcca gtcgacgtgc
     1441 tcttcctcgg aggagaggat ttcgcggaac aggtggcggc tgacgtaatc gccgctggac
     1501 tcgcagtgcg agatggcgtt cttgaggtcc ttgaggccgg cgagctccat cttgagatcg
     1561 cactcgagga tctccttgac gttctcgccg atcatcagct tgccgaggtc ctgcaggttc
     1621 ggcagcccct cgaggaacag gatgcgctgg gtcaactcgt cggcgtgctt catctcgtcg
     1681 acggattcgt ggaactcctt gtccgcaagc ttctccaggc cccagtcctt cagcatgcgc
     1741 gagtgcagga agtactggtt gatggccgtc agttcgttct tgaggatctt gttgaggtgc
     1801 tcgatgacct tggcgtcgcc tttcatgcgg tgctcctgct cggttcgggt ggcgcagtcg
     1861 gcatattaga ccatgtcttg acgcggcgtg gcggcggacc acgaagcttt gtcttgacgc
     1921 gcggtggtgc ggagcacctc gtcatgccga gccatgatga ctatgaactg gtgcgggcgg
     1981 ggaacttgcg ccgacagggc gccgttcgct caggcggcgt cgggatggac cgactggcgc
     2041 acgccggtca tggcgccgga ggcggcgggg gcgcgttcga gcatctcgct ggcgaagcag
     2101 gcgcactggc cgcagccggt ggccactccc agttcgcggc ccaggtcttc gatgctgcgg
     2161 gcgccgcgcc cgatggcgtc gtggatctgg tggtcggtca cgcgtttgca gatgcagaca
     2221 tacatgggcg gttcacctcg tggccttgag gcgaactatg gatgcaaatg cgaatgattg
     2281 tcaatacgta aagtacttat tctggccggg cttggccggg cctgcggtgg tccggctggt
     2341 gaggcgacca aaaaaaacgg ccccagactc catcctggag gccggggccg cgacgcgtgg
     2401 gggaacacgc atttggggaa cgtttggggt ttcgctccga acgcttaagt agattcaagc
     2461 cgtccggaaa agttcccctt tgggtcggac cacgataagt agctggaaaa taaaaagata
     2521 ttgtcgaaac tcagcgattt atagcgctta gcgaacggat tcttggccaa attctggtct
     2581 ctaaggccgg gatcatccgg tcctgaccgg gttccggttg tccttacagc aacggtttcg
     2641 gcccaaaatc tcgcttctaa ggccccgaat tcgcatcatt caaaatcata aacctcatta
     2701 aaacaataac ttgcccctga atgtgcgggt ccgacccgcg cggggcccgc gcggagtcag
     2761 aaagacgcct gcacgcccac catcgccagg cggtcgtggc ccgggcgcac gccgttgggc
     2821 gaacgcgagg cgatgtactc ctcgtcgaac aggttctgca cccgcgccag caggctgaag
     2881 tgccgggtga gccggtagga cgcagcgagg tcgaccacga aggcggagtc cgtccgctcg
     2941 ttcgccggga tcgcgccagt accggcgaca gtgcgcgtct cggactggta ggcgaagttc
     3001 aggttcagct ccagccggtc gccttccagg ccgacccgga tctggccgac gtgctcgggc
     3061 agatacggca tgcggtcgcc ggcttcgact tcgccccagg gcgcaaagct gctggcgaag
     3121 ctgttgtcga actccgcgtc gggcgtccac gtccaggcca gccgcgccgg cacgcgccag
     3181 ccgccggcga actcccacag caccgtctcg cccaacaact cgatgccgcg caccaccgcc
     3241 tcgccggcct cgaactggtc gccgatctcg ccaccgccgc cggtcgactc cgtgacggtg
     3301 cccacgaggt tgccgtagtc ggtgatgaaa ccgatggctt ccgcgtagta acgggcgcct
     3361 tcatagcgca caccgaattc ccagttggtg ctggtctcgg caccggcggt gctgccgggc
     3421 gacgaaggat taaagccctc gtgtacgccg ccaagcagct tgacgcggtc agtcagcgca
     3481 taagtggcgc cgatacccgt gatccactcg gtcgctgaac tttgccgcac ctgggtcggg
     3541 ccctcactgc gatccggcac cgtgcgtgag tagtcgctgc gcttcaatct gatgtgctcg
     3601 tagcgcacgc ccggcgacac gcgcaaggcg cccacgtcga tgtcggccgc gatgaagacg
     3661 gagctcacgt cggcttcccc tacgcggttt tcctgtccgc ccgcgatgcc ggcggtcgtc
     3721 agcacgaggt tgttgtcctc catgcggtag cggtcttcct tctggaagcg atcctcgaag
     3781 tcctcgtgga agcggaaacc ggccttcagc ttgactgccg cagcgcccgc atcgatgtca
     3841 tattcgccgc ggccctgcag gcccttcgag ccgtaggtgc gatagttgtc gcgcgcgcgc
     3901 agcgcgtcgt cggggctggt ggcgcccttg agccagctca gctggtcggc atagttgccg
     3961 gggtcttcca ggatggcgct gaggccgatg ccgccaacgt tgttgacgcg gtaccagctg
     4021 cgggcaaact cgttgtcgta gtaggtcagc gaggccttcc agggcatcgc ggcgggttcg
     4081 atcagccagc tcacctggcg cagcttgaac tcggcgtcga gctggtcgat ctgcgtggcg
     4141 gcgtacaggc ggaaggggtc cgcggcgtag tcctcatcgg tcaggccgag gtaagactgc
     4201 tgcgcgtcct gctcggcacg gccgtacttg aactccagtg cctggtatat ccgggcgccc
     4261 gggtcggtat tcacgcgggc cttcagcaag atgtcgtcga agtcgtagcc ggcggtggtg
     4321 ttgcggtcgt tcgggtcgat ggtcttgaag ccgtccgact gctgctgcac ggtttcgaac
     4381 agcaggccga agtgatcgcc gctgccgccc gcgatgatgc gcgcgtcatg cgtatcgttc
     4441 tgaccataga aataatcggc gtacatgccc cactcgttgg ggatcggcgt ggacagcacg
     4501 ttcagcgcgc cgccgatggt gcgcgggccg accgggatcg atgccgggcc cttcagcact
     4561 tcggccgcgt agacgcggcg cgtgttgggg aagtagtacg ccgcgggggc ggagtagggc
     4621 gccggtgcga tcagcacgcc gtcctcgagc acggcgatgc ggctgctgcg gtcctggccg
     4681 gagccgcgga tgccgatgtt ggggcgatgg ccgaagcctt cctcgtcctg caggtacacg
     4741 ccggggacgc tgcgcagcac gcggtggatg tccgagtact tgaactcttc gagttcgtcg
     4801 gggccgatga agtgcgcgga gccgccgatg tcgcgcgccg cggcggccga cccgacgatg
     4861 gtgacgctgc cgagctgggc cgggtcgagc ggcgggatgg cggaaagcac gagcggcgtc
     4921 gtggtttccg tttcgtcggt cgcatcggcc tgggcgacga cgagcgtggc gtcgtccgcg
     4981 agggccgggc cggcgaaggc agcgatcgaa agcagggcga gcgggtgagc ggggcgtggc
     5041 aagcgcatgg aaactccctg agatgcgttg gcaaggttgt tgttgatgcg cacactaacg
     5101 ataatgcgaa cgattcgcaa tgggatttac cgaactgctt ggtcggaccc tcggaagtat
     5161 ttgttagata tcgaaaaatg gctgagaatg gggcatttcc tggccttaga ggtgccgatt
     5221 tggggcgaat ttggcgatct aagcggcgtt ggttccacgc ttgccccacg ccctacctgg
     5281 cccggaacag cttgagcctc cgttagatcg cgttttcgcg gctgaaatca cccttctacg
     5341 ggctgaattt cggggtcatt gcatggaaag tcctgtttga acaattactt gtcagggtcc
     5401 gacccgcggg gaccgcgggg attgctgccg ccgcgcgacc gcctgcggcg catcctgctg
     5461 ctggcgctgc cgatcatcgg cggcatggtg tcgcagaacg tcatgaacct ggtcgatacc
     5521 gccatggtcg gcacgctcgg cgacgccgcg ctggctgccg tcgggctcgg cggcttcgcc
     5581 aacttcatgt tcatggcgct gctgctcggc ctgtcggtcg gcgtgcagac cacggcgtcg
     5641 cgccgcaagg gcgagggcaa gctcgacgag acggcgatcc cgctcaatgg cggcctgttc
     5701 atcgtgctgt gtatcgcgcc gccgttgtcc gcgttgttgt actgggccgt gccgcacatc
     5761 tacccgcacc tcaacagcga ccccgacgtc atcgcgcaag gtgtgccgta cctgcaggcg
     5821 cgtgtgctcg cgatgctgtt catgggcgcg aacttcgcgt tccgcggcta ctggaacgcc
     5881 gtcgacctgg cgcggctgta catgaacacg ctgatcgcca tgcacgcctg caacattttc
     5941 ctcaactggg tgctgatctt cggcaacctc ggcgcgcccg cgctcggcac gcagggcgcc
     6001 ggcatcgcct cggcgctcgc gacggtggtc ggctgcggcc tctacatctg gctgggtttc
     6061 cggcatgcgc gcagcgccgg cttcatgcag cggatccccg cccggcacaa cctgctcgcc
     6121 ttgttccgcc tgtcgctgcc caccggcatc cagcagctgt tcttctccac cggcttcgtc
     6181 ctgaccttct ggatcatcgg ccaggtcggc acgcgcgaaa tggcggcggc caccgtgatc
     6241 ctcaacctga cgctggtcgc cctgctgccg ggtctgggcc tcggcctcgc cgcggcgacc
     6301 ctcgtcggcc aggcgctcgg tcgcagcgac gtcaatgacg cgtcgcgctg gggctgggac
     6361 gtcgccaagg tcggcatgct cgggctgctg ctgctcggcc tgccgggcgc catctggccc
     6421 gagctgctgc tcggcgtgtt catccacgat ccggacacca tggaggtagc gcgcttcccc
     6481 atgcggctgg tcgggctcgg catggccatc gaaggactcg gcgtggtgat gatgcatgcg
     6541 ctgctcggcg ccggcgacac gcagcgcgtc atgaaggtcg ccgtgctggc gcagtgggcc
     6601 gccttcctgc cgctggccta cctggccggg ccggtgctgg gctttggcct gaccttcatc
     6661 tggtgcatgc aggtgggcta tcgcgcggcc atggccggcg tattcacgct gttctgggtg
     6721 cggcgggggt gggcgcggat caaggtgtag ccgtcgctcg ggtcggaccc tggcaaccac
     6781 ctgaaaaccc gagagaaatg gcgataggag ggctgattca gaccgttaga gcagtgcttt
     6841 tcgccagaaa tcgggtctct aaacgagttg ccggggtcct ggcagtggtc gcgcgtgcct
     6901 tagagcacgg aattagccag gaaacctgcg ttctaaggcg cgggatggcg gctatttctt
     6961 gctggaagct tcctgcaaac aatggcttgc acagctgccg cgggtccgac ccggaatcag
     7021 cggcgcagcc gctcgatttc ctcaagcaag tccagcgcca gcgcaacgcc ggggaggttg
     7081 acgtccaggt cgtggtgcag ccgcacggcg acctgggtgc ggcgcacgct gcggccggtg
     7141 aagcgccact gcccgcgctg ctcgccacgc ggctcgatca cgccctcggc caccagcgcc
     7201 gttacgaatt cctcgctggc atgacagatc cggcaaaact cctcgaactc gagctcgagc
     7261 gattcgtcga gcagctggcc catgaggatg tccatctcgt cccgggtcat cggctcagcc
     7321 ctcgatgtcg cgccgcgggt tgaacggcat cttcttgcgc atctcctcgt agagcgtgcg
     7381 cgcgaaggac gtgtcggccg gcggtgtgtc gatgcgcagt gtgacgatct ggtcgcccgg
     7441 cggcgtaccg ggcaggccgc gccccttgag ccgcaggcgg cggccggact gcgagccggc
     7501 ggggatgcgc agttcgacct tgccgcccag ggtcggcacg ccgacggtct cgccgagcgc
     7561 ggcttcccac ggggcgacgg gcagcgtcag caggatgttg cggccctgcg cctcgaagat
     7621 cgggtggggt tcgaacatga cctcaaggaa caggtcgccg gccgggccgc cgttggcgcc
     7681 gggggtgccc tggccggcca ggcgaatgcg ctggccctcg gtgacgccct tggggatcgc
     7741 cacgcggatg ttgtgcttac ccttgcccgg cgagcggacc tcgagcgtgc ggttggcgcc
     7801 gtggaaggcg tcttccagcg agatcttgac gcgtgcgctc tggtcctcgc cgcgcatcgg
     7861 gccggcctgg cggtagtgga acgcgccgcc ggccgggccg gcgcccgggc cgccgcgcgc
     7921 gccgccggcc atgccgccgc ggccgaacag cgactcgaag aattcggaga agccgccgaa
     7981 ctgttcgccg ccggggccgc cgcctgagga gtagtagtac tcgcgcgcgc cgccgcccgg
     8041 aggcggccgg aattcctggc cggccttcca gtcggcgccg agctggtcat aggcggcgcg
     8101 cttttccggg tccttgagta cctcgtaggc ctcgcccact tccttgaagc gcgcctcggc
     8161 gtcggcctcc ttgctgacgt cgggatggta cttccgcgcc aatttccggt aggcccgctt
     8221 gatctcctcg ccgctcgcgc cgcgctcgac gcccagcgtc gcgtagtagt ccttgaaatc
     8281 catataccct cgggtcggac cctcgaatcc ctatgtattc aaaagcaatt tacgaaccga
     8341 accccttcct gggagccttg gaacaacgat tctgcggcca aatcggcatt ctaagccaac
     8401 ccctttggca aaacccgcac atgacctcgt cggccttaga agcacgattt cggctggaaa
     8461 aatgcatcct aaggcctaaa gttgagggcg tttcgcggga attcaactga caaacaatgg
     8521 tttacgacgg tccgacccga aagcgtaaaa aaaatcgaca ggcggtgtcg ggattctttc
     8581 caggaactga gaaaagggtc acacttcccg gcggggaacg ggccacaata cggcggacgc
     8641 aatcaaggac gagattgtct cgatgctgat cggatccgtg gggtacgggc tggcggcggc
     8701 ggcgttcggc gcgctcgcgc tgttgatgct caccagctgg cggggccgcc tgccaggcgg
     8761 cctgcagctg cacccggtgg ggaatgtcga ggtccgacgt gccgatgccc cactcgtcgg
     8821 cgccgacgcc ggtgccaagc atcaacgcac gatcggcggg gtcggccgcc atgaccgccg
     8881 cctgggccgc ggccgctgcg ttcacccccg agacccgcgc ccactggcag ctcgtcggcc
     8941 tgctggagat cgccagcaat accgcctggc tggtgttcct cgccaggctg agcccggccg
     9001 gccagggcct cgacggcgcc cgccgccagt tcatcgccca cgcccccgcc gcgttcgtct
     9061 tcctctacgg caccatcatc atcgccggcg cgcgcggcga cgcgaccatc ccggtcctca
     9121 cccaggcgct gatcatcacc ggcctggtga ccgcgatcgg cggcctggtg ctgctcgaac
     9181 aggccttccg cgccatgcgc gccgcgcccc gccagcagat cctgccgctg atcatcggcc
     9241 tcggcggcat cctcgcctac gacctgttcc tctattccca cggcatgctg ttcctcggcc
     9301 tgcacgaaga gctgtggggc gcgcgcggct tcgccaacct gatggccgtg ccgttcctgc
     9361 tgctggccgc acgccgcaac cccgactggt cggtggacgt gttcgtttcg cgccacgtcg
     9421 cgttctacac caccagcctg gtcggcatcg gcgcctacct gctggtgatg gccgtcgccg
     9481 gctacggcct gcgcgtcgcc ggcggcgagt ggggcttcat cttccaggtg atcttcttct
     9541 tcggcgcgct ggtggtgctc gcctgggtgc tgttctcgcc gagcgcgcgc gccggcctgc
     9601 gcgtgttcct cgccaagcat ttctacagca acaagtacga ctaccgcgaa gagtggctca
     9661 agctcaccca gcgactcgcc gacgcctccg gccgcgaatc gcccgccgtc cgcggcctcg
     9721 gcgcactcgt cgacatggtc ggcgcggacg gtgccatcct gtgggagcgg acgggtcaag
     9781 aaccggccgc gccgtccgac gccgaccccg acgaaccgcc gatcgacgac atcgccagct
     9841 tccagcgcac cgccaccacc ggcagtgtcg gcgcccaggc cacgctgccc cgcacgctcc
     9901 aagccgccga ccccgtcgcg acgttcctcg gcgagcgccg ctggaccatc aacctctcgc
     9961 aggcgcgccg caaccccgac tcgcatcccg gcctggccct gccaccgtgg accgaccagc
    10021 tcggtaccga ggcgctcgtc gtgccgctgc tcaaccgcga ccgcctgcag gccatcgccg
    10081 cgctgctcaa ccccagccgc gtcggccgcc t
//
LOCUS       DAOMPG010000075        10061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2887_length_10061_cov_4.432840, whole genome shotgun sequence.
ACCESSION   DAOMPG010000075 DAOMPG010000000
VERSION     DAOMPG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_10061_cov_4.432840"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PLS34_05710"
     CDS             complement(<1..167)
                     /locus_tag="PLS34_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005476509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRP87000.1"
                     /translation="MFENLSDRLSRAGDALKGRGRITEDNIRDTLRQVRMALLEADVA
                     LPVVKDFIERTR"
     gene            308..1117
                     /gene="ccsA"
                     /locus_tag="PLS34_05715"
     CDS             308..1117
                     /gene="ccsA"
                     /locus_tag="PLS34_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRP87001.1"
                     /translation="MPIVVFVLYLASGTLLLRKQLADAPGANMTTGIGPALLAGAAGL
                     ALHSLALAGLISERGGPELSIGGVLSLVAWLLALFALLAALKPRFVGLAAVLLPAAGL
                     AALAALLPDPVAARGGAPDWGIHAHVTLSILAYSLLSLGAGLAILFLYRERTLRRRQL
                     NAWTRILPPLESLESALFTALVAGFSLLTLALFSGLMFLEDIFAQHLLHKTVLSIVAW
                     GVFAVLLFGRLRLGWRGRQAVHWVLAGYGLLALSYFGSRIVLEFILGRQWG"
     gene            1133..2410
                     /locus_tag="PLS34_05720"
     CDS             1133..2410
                     /locus_tag="PLS34_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HRP87002.1"
                     /translation="MNDVPLGTLFSLLGVLLVLSAFFSGTETALMSINRYRLRHRARQ
                     GSRPAKLAEALLKRPDRLIGLILLGNNFVNIFASSLVTVIALRIGGEGAIAIGAGLLT
                     LVILIFSEVAPKTLAALHPERLALPAALVYYPLLKVTYPIVWLVNLMANGVLRLLGIR
                     AEQTQGHALSHEELRTVVNEAGALIPKRHQRMLISVLELQDVSVDDIMIPRNEIDGLD
                     LEDDWDDLLEQIAESQHTRLPVYRGDLDHVVGILHLRSIMSELADDELTREHLAAKAL
                     EPYFVPEGTSLHTQLLNFQRAKRRLALVVDEYGDIQGLVTLDDILEEIVGEFTTDPAD
                     IYRDVQQEADGSFMVSGGANIRELNRMMGWRLPTDGPKTLNGLIVERLETIPEAGSQL
                     QLSGYQLEVVQTADNAVRSVRLRPPPRAVAPVA"
     gene            complement(2407..3753)
                     /gene="radA"
                     /locus_tag="PLS34_05725"
     CDS             complement(2407..3753)
                     /gene="radA"
                     /locus_tag="PLS34_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRP87003.1"
                     /translation="MAKARTMFVCNECGGTSPKWVGQCPDCGRWNTLEESREAPRVRA
                     VGWAGDAPRLISEVEAEVEARWPTGIGELDRVLGGGLVPGSATLIGGDPGIGKSTLLL
                     QAAVRLAGELPALYVTGEESLRQVGMRARRIGMQAGGLQLLAETNIERLLAQAETVKP
                     RVLVVDSIQTMHTDALQSAPGAVAQLRESSARLVRYAKQGGTAVFLVGHVTKDGAIAG
                     PRVLEHMVDTVLYFESDVGSRFRIIRAVKNRFGAANEIGVFAMTEQGLREVPNPSSIF
                     LSGHDGNVSGSAVMVAREGSRPLLVEVQALVDETRSGHPRRVSVGLDSTRLALLLAVM
                     HRHAGIVTYDQDVFVNVVGGVRIMETAADLPVLLAALGSLRDRPLPGGLVSFGEVGLA
                     GEIRPVPYGEERLREAAKHGFKRAVVPAANAPRGRLAGIEVVRVKRLSEAIEAAFG"
     gene            3920..4744
                     /locus_tag="PLS34_05730"
     CDS             3920..4744
                     /locus_tag="PLS34_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRP87004.1"
                     /translation="MSLKGKIRYFEFTHTGRVREHNEDAVFADMDVGLMVLADGMGGY
                     NAGEVAAELAIKTVVDMVRAGVERETRTSVDDSSGLMRQSIVLRNAIIRANKIIWQTA
                     QTQPQCRGMGTTIVACLFHDDRVSIAHVGDSRVYRLRKNRFEQLTLDHSLMQELVDRG
                     FFSHEEALRSANRNYVTRALGVEPTVDAEVQEETVKPDDVYVLCSDGLSDMVEEEDIH
                     LTISTFSANLQTGGEQLIQLANANGGRDNVSVCLAQVVESFAEARGLVGRLKGWFG"
     gene            4759..5448
                     /locus_tag="PLS34_05735"
     CDS             4759..5448
                     /locus_tag="PLS34_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRP87005.1"
                     /translation="MARMILSLNGQTLAEYNLSKERYTVGRLPDNDIRIDNPAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHALANGDVVTIGHHQLRFVDSGVDDSQQ
                     EFEKTMVISTSKREMERLQALAGEHVDDEAAPPPLLPGSPARLQVLTGAFAGRELVLK
                     KPLTTLGRPGIQVVAITRRGETYSMVTVERGQRGNPPMVNGEAVGSGARRLNDNDVLE
                     IAGVKMGFFVN"
     gene            complement(5470..6546)
                     /gene="alr"
                     /locus_tag="PLS34_05740"
     CDS             complement(5470..6546)
                     /gene="alr"
                     /locus_tag="PLS34_05740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008607117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRP87006.1"
                     /translation="MTRAARATVDAAALQHNLATVRRLAPRTRVMAVIKANAYGHGLV
                     GTARALREADAFAVARVEEAVALREAGLQNPVVLLEGVLGAAQLPLAASLGLQLVVHS
                     PEQLAMLEAAELSRPIEAWLKLDTGMNRLGFPPAEARTALRRLAACPAVVPQPRLMTH
                     LACADERDAEVTDAQLRRFAEALGELPGERSVANSAGLLWRPDSHADWVRPGIMLYGV
                     SPFADQVGADLALRPAMQLMSTVIALRTVRRGERVGYGGVWTAREDSRLAIAAIGYGD
                     GYPRQLGSGTPVLVRGRRTPLAGRISMDMIAVDVTLVPEVRVGDEVMLWGAGLPVEEL
                     ARMAGTIPYELLCGVTQRVGVTMT"
     gene            complement(6554..7948)
                     /gene="dnaB"
                     /locus_tag="PLS34_05745"
     CDS             complement(6554..7948)
                     /gene="dnaB"
                     /locus_tag="PLS34_05745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRP87007.1"
                     /translation="MVGTPESVFPARSPEALRVPPHSVEAEQAVLGGLMLDNSAWDTI
                     ADAVADTDFYRRDHRLIFKAIGTLAERGEPSDAVTVSELLDRQGDLEHAGGLAYLGQL
                     ARETPSAANIRAYAAIVRERAMLRELIRIGGEIATSAYQPEGRDASQLVDEAERQVFE
                     IAEQGKRRGSGFQPLKRILGATIDRLDELQKNQGDVTGLGTGFKDFDSMTAGLHPGDL
                     VIVAGRPSMGKTSFAMNIAEHAAIGAGASVAIFSMEMSAEQLSFRMISSLGRVNQSNL
                     RRGRFSDEDWSRINSAVSVMSKARVFIDDTPALNPTELRARARRLKREHGLDLIVVDY
                     LQLMQVHGSAENRATEISEISRSLKALARELEVPVITLSQLNRSVEQRTDKRPVMSDL
                     RESGAIEQDADLIAFIFREEVYNKDTPRKGIADVIISKQRNGPIGDVQLTFLGEYTKF
                     ENFMPDAYGSGPFG"
     gene            complement(8035..8484)
                     /gene="rplI"
                     /locus_tag="PLS34_05750"
     CDS             complement(8035..8484)
                     /gene="rplI"
                     /locus_tag="PLS34_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRP87008.1"
                     /translation="MEVILLDKVENLGGIGDKVKVKPGYARNYLLPQGKATRATPENI
                     AAFEERRAELEAKAAAVITEAKARAEKIDGLKLVIKAKVGSEGKLFGSIGPVDIAEAA
                     ELAGVTIERSELRLAEGPLRTVGEHEVEVHLHAEVSVPITVTVEAEE"
     gene            complement(8532..9407)
                     /locus_tag="PLS34_05755"
     CDS             complement(8532..9407)
                     /locus_tag="PLS34_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87009.1"
                     /translation="MLEFARWITERDYRAALMAAGLVLLPLLAPASCAVLALVALQRG
                     AASAWLAGGIAAALLAVVAALSGAHPVAGVLSAVTIWAPTLAVTQLLISTGSLSAAAR
                     VATTGAVVLAGGWSLMAPTQGEPWVGLVTEMVAPLAAQTGTDAALMAERLLALLPGII
                     AASLLLVSLGGMFIAMWLRAGLAQPGAFGEAFRALQLGPVVGGVGALATVGVLAGGQA
                     VAGPVALPLGAALLLQGIAVMHGLVRIKGMHRAWLFAGWTALVFLSPWTLVGFAFYGM
                     ADTFMDLRRKAAGKV"
     gene            complement(9457..9681)
                     /gene="rpsR"
                     /locus_tag="PLS34_05760"
     CDS             complement(9457..9681)
                     /gene="rpsR"
                     /locus_tag="PLS34_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRP87010.1"
                     /translation="MSRFIRRRKFCRFTADKVVEIDYKDLATLKAYVTETGKIVPSRI
                     TGTNSNYQRQLAVAIKRARFLALLPYTDRH"
     gene            complement(9696..>10061)
                     /gene="rpsF"
                     /locus_tag="PLS34_05765"
     CDS             complement(9696..>10061)
                     /gene="rpsF"
                     /locus_tag="PLS34_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRP87011.1"
                     /translation="RYRGMIEGDGGTIHRQEDWGRRQLAFPIQKVHKAHYVLLNIECS
                     KATLDELTGAFRFNDAVLRHLVIARDEAVTEPSPMAKPEDEREGRSRRGRDDDDDDDS
                     DDDDRDEDARPSRRRASQD"
BASE COUNT         1548 a         3563 c         3357 g         1593 t
ORIGIN      
        1 cgggtgcgct cgatgaaatc cttcaccacc ggcagggcga cgtcggcctc gagcagcgcc
       61 atgcgcacct ggcgcagggt gtcgcggatg ttgtcctcgg tgatgcggcc gcgccccttg
      121 agggcatcgc cggcgcggga cagacggtcg ctcaggttct cgaacatcgc tcttggggcg
      181 ctctgggact ggccgggggt gcggattata cccgtggtcg gcgccgcttc ggctttcccc
      241 gtttctaaac cgccgtggcg ctgtgtcaga atgcggcttt agcctggtgg ccgagcctcg
      301 cctgatgatg ccgatagtcg ttttcgttct ctacctggcg tccggaacgc tgctgttgag
      361 aaagcagctg gcggacgccc ccggcgcgaa catgaccacg ggcatcggcc cggcgttgct
      421 ggcgggggcc gcgggccttg cgctgcactc gctggcgctc gccgggctga tcagcgagcg
      481 cggcggcccg gagctatcga tcggcggcgt gctgtcgctg gtggcctggc tgctggcgtt
      541 gttcgccctg ctggccgcat tgaagccgcg cttcgtcggc cttgcggccg tgctgctgcc
      601 cgcggccggg ctggccgccc tcgcggcact gttaccggat cccgtggccg cgcgcggcgg
      661 ggccccggac tggggtatcc atgcgcatgt aacgctttcc atcctggcct acagcctgct
      721 gagcctgggc gccggcctgg caatcctgtt cctgtaccgc gagcgcaccc tgcggcgccg
      781 gcagctgaac gcatggacgc gaattctccc accgctcgag tcgttggaga gcgcgctgtt
      841 cacggcgctg gtcgcgggat tctcgctgct caccctggcg ctgttcagcg ggctgatgtt
      901 cctggaggac atcttcgccc agcacctgtt gcacaagacg gtgctctcga tcgtcgcctg
      961 gggcgtgttt gccgtcctgc tgttcggccg cttgcggctg ggctggcgtg gccgccaggc
     1021 cgtccactgg gtgctggcgg gctacgggct gctggcgctt agctatttcg gcagccgcat
     1081 cgtgctggag ttcatcctcg gccgccagtg ggggtaggag ggaagacccg ccttgaatga
     1141 cgttccgcta ggcacgctgt tttcgctgct cggcgtgctg ctggtgctgt cagcgttctt
     1201 ctcgggcacc gagacggcgc tgatgagcat caaccgttac cggctgcgcc accgcgcccg
     1261 ccaggggtcg cggccggcca agctcgcgga ggcgctcctg aagcgcccgg accgtctcat
     1321 cggcctgatc ctgctgggca acaacttcgt caacattttc gcctcctcgc tggtgaccgt
     1381 gatcgccctg cggatcggcg gcgagggcgc catcgccatc ggcgccgggc tcctcacgct
     1441 ggtgatcctg atcttctcag aggtcgcgcc caagacgctg gccgccctgc acccggagcg
     1501 cctcgcgctg cccgccgcgc tggtctatta cccgctgctg aaggtgacct acccgatcgt
     1561 ctggctggtg aacctgatgg ccaacggcgt cctccggctg ctcggcatcc gggcggagca
     1621 gacgcagggc catgccttgt cgcacgagga actgcgcacc gtggtcaacg aggccggggc
     1681 gctgatcccc aagcggcacc agcgcatgct gatcagcgtg ctggagctgc aggacgtcag
     1741 cgtcgacgac atcatgatcc cgcgcaacga gatcgacggc ctcgatctcg aggacgactg
     1801 ggacgacctg ctggagcaga tcgccgagag ccagcacacg cgcctgccgg tctaccgagg
     1861 cgacctggat cacgtcgtgg gcatcctcca cctgcgcagc atcatgagcg aactggcgga
     1921 tgacgaactc acccgtgagc acctggccgc gaaagcgctg gagccctatt tcgtacctga
     1981 aggcacctca ctgcacaccc agctgctcaa ctttcagcgc gccaagcggc gccttgcgct
     2041 ggtggtggac gagtatggcg acatccaggg actcgtgacg ctggacgaca tactcgagga
     2101 gatcgtcggc gaattcacca ccgatccggc cgacatctat cgcgacgtgc agcaggaagc
     2161 cgacggcagt ttcatggtca gcggcggcgc gaacatccgc gagctcaacc gcatgatggg
     2221 ctggcggctg cctaccgacg gcccgaagac cctcaacggc ctgatcgtgg agcgactcga
     2281 gaccattccg gaagccggct cccagctgca gctgtcgggc taccagctcg aggtcgtgca
     2341 gaccgccgac aacgcggtcc gctctgtgcg gctgcgcccg ccgccccggg ccgtcgcgcc
     2401 ggtcgcctag ccgaaggccg cctcgatggc ctcgctgagt cgcttcaccc tgaccacctc
     2461 gattcccgcg agccggccgc ggggcgcgtt cgccgccggt acgacggcgc gcttgaagcc
     2521 gtgcttggcg gcctcgcgca gccgttcttc cccatagggc accggccgga tctcacccgc
     2581 cagtccgact tccccgaacg acaccagccc gccgggcagt ggccggtccc gcaggctgcc
     2641 cagcgccgcc agcaacaccg gcaggtcggc tgccgtttcc atgatgcgca cgccgccgac
     2701 gacgttgacg aacacgtcct ggtcgtaggt cacgatgccg gcatggcgat gcatcaccgc
     2761 cagcaacagc gccaggcgag tcgagtcgag acccaccgag acgcgtcgcg gatggccgga
     2821 acgggtttcg tccaccagcg cctgcacctc gacgagcagc ggccggctgc cctcccgcgc
     2881 caccatgacg gcgctgcccg aaacgttccc atcgtgcccc gacaggaaga tcgaggacgg
     2941 attcggcacc tcgcgcaagc cctgctcggt catcgcgaac acgccgatct cgttggccgc
     3001 gccaaagcgg tttttgacgg cgcggatgat gcggaagcgg ctgcctacgt cgctctcgaa
     3061 atacagcacg gtatccacca tgtgctccag cacccggggc cccgcgatgg cgccgtcctt
     3121 ggtcacgtgc ccgaccagga acacggcggt gccgccctgc ttggcgtagc gcaccagccg
     3181 ggccgagctc tcccggagct gggccaccgc gcccggggcc gactgcaggg cgtcagtgtg
     3241 catggtctgg atcgagtcga ccaccagcac gcgcggcttg accgtttcgg cctgggccag
     3301 cagccgttcg atgttcgtct cggccagcag ctggaggcca ccggcctgca tgccgatgcg
     3361 ccgcgcgcgc atgcccacct ggcgcagcga ctcctcgccg gtcacgtaca gcgccggcag
     3421 ctcccccgcc aggcgcaccg cggcctgcaa cagcagcgtg gacttgccga tgcccggatc
     3481 gcccccgatc agggtcgccg aacccggcac caggccgccg ccgagcaccc ggtcgagctc
     3541 gccgatgccg gtcggccagc gcgcctcgac ttcggcctcg acctcggaga tcaggcgcgg
     3601 cgcgtcaccc gcccagccga ccgcgcggac ccgcggcgcc tcgcggctct cctcgagcgt
     3661 gttccagcgc ccgcagtcgg ggcactggcc cacccacttc gggctggtgc cgccgcactc
     3721 gttgcagacg aacatcgtcc tggccttggc catcgaatct cccgcgggct ccgcgacccc
     3781 ggcacggtac acagggggac gcagcgccgg caagcgcaac tcgcggcgtg cccattaatg
     3841 tgatgccctg ctcggacttg tggcgagggt gcattaagct gttcaattgc gggttagtgc
     3901 gctgcggaaa agtttttcaa tgagtctgaa gggcaagatt cgctacttcg agttcaccca
     3961 taccggccgg gttcgcgagc acaacgagga cgccgtgttc gccgacatgg acgtcgggct
     4021 gatggtgctg gccgacggca tgggcgggta caacgccggc gaagtggcgg ccgagctggc
     4081 gatcaagacc gtcgtcgaca tggtccgggc cggcgtggag cgcgagaccc gcacctcggt
     4141 ggacgacagc agcggcctga tgcggcagag catcgtgctg cgcaacgcca tcatccgcgc
     4201 caacaagatc atctggcaga ccgcgcagac ccagccgcaa tgccggggca tgggcacgac
     4261 catcgtcgcc tgcctgttcc acgacgaccg cgtgtcgatc gcccacgtcg gcgactcgcg
     4321 cgtctaccgg ctgcgcaaga accgcttcga acagctgacc ctcgaccact cgctgatgca
     4381 ggagctggtc gaccggggct ttttttccca cgaggaggcc ctgcgctcgg ccaaccggaa
     4441 ctacgtgacc cgggcgctgg gcgtcgagcc gaccgtcgac gccgaggtcc aggaggagac
     4501 cgtcaagccc gacgacgtct acgtgctgtg ctcggatggg ctgtccgaca tggtggaaga
     4561 ggaagacatt catttaacta tcagtacctt tagtgctaat ctgcagaccg ggggtgagca
     4621 attgatccag ctcgccaacg ccaacggggg gcgggacaac gtgtcggtgt gtctcgccca
     4681 ggtggttgag tccttcgccg aggcccgtgg cctcgtcggc agactcaagg gctggttcgg
     4741 ttagtaattg gggtagacat ggcccggatg atcttgagcc tgaatggcca gacgcttgcc
     4801 gagtacaacc tcagcaagga acgctatacg gtcggccggc tgccggacaa cgacatccgc
     4861 atcgacaatc cggcggtcag cggccaccac tcgctgatca tcaacatcct caacgactcg
     4921 ttcctcgagg acctcaacag caccaacggc acctacgtca acggcaagct gatcaagaag
     4981 catgcgctgg ccaacggcga cgtcgtcacc atcggccatc accagctgcg cttcgtggat
     5041 tccggcgtgg acgacagcca gcaggagttc gagaagacca tggtcatctc gaccagcaag
     5101 cgcgagatgg agcggctgca ggccctggcc ggcgagcacg tggacgacga ggctgcgccg
     5161 ccgccgctgc tgccaggttc cccggcccgc ctgcaggtgc tgacgggcgc cttcgcgggc
     5221 cgcgaactgg tgctgaagaa gccgctcacg acgctcggcc gcccgggcat ccaggtggtc
     5281 gcgatcacgc ggcgcggcga gacctattcc atggtgaccg tggagcgggg ccagcggggc
     5341 aacccgccga tggtcaacgg cgaagccgtc ggctccggcg cgcggcggct gaacgacaac
     5401 gacgtgctcg agatcgccgg cgtcaaaatg ggcttcttcg tcaattgaag cccgggggcg
     5461 ccgccgtgct cacgtcatgg tgacgccgac gcgctgggtc accccgcaga gcagctcgta
     5521 gggaatggtg ccggccatcc gcgccagctc ctccaccggc agtcccgcgc cccacagcat
     5581 gacctcgtcg ccgacgcgca cctcgggtac cagcgtcacg tccaccgcga tcatgtccat
     5641 ggagatgcgc cccgccagcg gggtgcgtcg gccccgcacc agcaccggcg tgccgctgcc
     5701 cagctggcgc ggatagccgt cgccgtagcc gatcgcggcg atggccagcc ggctgtcttc
     5761 acgggcggtc cagacaccgc cataaccgac ccgctcgccg cggcgcaccg tacgcaatgc
     5821 gatcacggtg ctcatcagct gcatggccgg ccgcagcgcc aggtcggcgc cgacctggtc
     5881 ggcgaacggc gacacgccgt agagcatgat tcccggccgc acccagtcgg catggctgtc
     5941 gggccgccac agcagcccgg ccgagttcgc cacgctgcgc tcgcccggca gctcgcccag
     6001 cgcttcggcg aagcggcgca actgggcgtc ggtcacttcc gcatcgcgtt cgtcggcaca
     6061 ggccaggtgc gtcatcaggc gcggctgtgg aacgacggcg ggacaggctg ccagccgccg
     6121 cagcgcggtg cgagcctccg cgggcgggaa gcccagccgg ttcatgccgg tgtccagttt
     6181 cagccaggct tcgatcgggc gcgacagctc ggcggcctcg agcatggcga gctgttccgg
     6241 cgagtgcacc accagctgca gcccgagcga ggccgccagc ggcagttgcg cggcgcccag
     6301 caccccttcg agcaggacga cgggattctg caggcccgcc tcgcgcagcg cgacggcctc
     6361 ctcgacgcgg gcgacggcga aggcgtccgc ctcgcgcagc gcgcgtgccg tgccgaccag
     6421 gccgtggccg taggcgttgg ccttgatgac cgccatcacg cgggtgcgcg gcgccaggcg
     6481 ccggacggtc gcgaggttgt gttgcagggc cgcggcgtcg accgtggcgc gggcggctcg
     6541 ggtcacgcgt cggtcacccg aaagggccgc tgccgtatgc gtcgggcatg aaattctcga
     6601 acttggtgta ctcgccgagg aaggtgagct gcacgtcgcc gatcggcccg ttgcgctgct
     6661 tggagatgat cacgtcggcg atgcccttgc gcggcgtgtc cttgttgtag acctcctcgc
     6721 ggaaaatgaa cgcgatcagg tcggcgtcct gctcgatggc cccggattcg cgcaggtccg
     6781 acatgaccgg gcgcttgtcg gtgcgctgct cgaccgagcg attcagctgc gagagagtga
     6841 tgaccgggac ttccagttcg cgcgccagcg ctttcaggga gcgcgagatc tcggagatct
     6901 cggtggcgcg gttctcggcg ctgccgtgca cctgcatgag ctgcaggtag tccaccacga
     6961 tcaggtcgag gccgtgctcg cgtttcaggc ggcgtgcacg ggcgcgcaat tccgtggggt
     7021 tcagcgccgg ggtgtcgtcg atgaacaccc gcgccttgga catcaccgag accgcggaat
     7081 tgatccggct ccagtcctcg tccgagaaac ggccacgcct gaggttcgac tggttgaccc
     7141 ggcccagcga ggaaatcatg cggaacgaca gctgctcggc ggacatttcc atgctgaaaa
     7201 tggccaccga cgcgcccgcc ccgatggcgg cgtgctcggc gatattcatc gcgaaactgg
     7261 tcttgcccat cgatgggcgg ccggcaacga tcaccaggtc gcccgggtgc aggccggcgg
     7321 tcatgctgtc gaagtccttg aagccggtcc ccagcccggt gacgtcgccc tggttcttct
     7381 gcagctcgtc gagccggtcg atggtggcgc cgaggatgcg cttcagcggc tggaagccgg
     7441 agccgcgccg cttgccctgc tcggcgatct cgaacacctg gcgctccgcc tcgtccacca
     7501 gttggctcgc atcgcgacct tccggctggt aggcgctggt ggcgatctcg ccgccgatgc
     7561 ggatcaactc gcgcagcatg gcgcgctcgc ggacgatggc ggcgtaggcc cggatgttgg
     7621 cggcgctggg cgtctcgcgc gccagctgcc cgaggtacgc caggccgccg gcgtgttcca
     7681 ggtcgccctg gcggtcgagc agctcggaca cggtgactgc gtcggagggc tcgccgcgct
     7741 cggccagcgt accgatggcc ttgaagatca gtcgatggtc gcggcggtag aaatccgtgt
     7801 cggccaccgc gtcggcgatg gtgtcccagg cgctgttgtc gagcatgagc ccgcccagca
     7861 ccgcctgctc ggcctcgacc gagtgcggcg ggacgcgcag cgcctcgggg ctgcgcgcgg
     7921 ggaaaaccga ttcaggcgtg ccgaccaacg cgctctacct ggcagggggc ggaatgaccg
     7981 ccaccatagc acagcaaaat ggtcgcggag ccagcgcccc gcgacgcccg gaaatcattc
     8041 ctcggcttcg accgtgacgg tgatcgggac cgacacttcg gcgtgcagat ggacttccac
     8101 ctcgtgctcg ccgaccgtgc gcagcggccc ctcggcgagg cgcagctcgc tgcgctcgat
     8161 ggtcacgccg gcgagctcgg ccgcctcggc gatgtccacc gggccgatcg acccgaacag
     8221 cttgccctcg gagcccacct tggccttgat gaccagcttg agcccgtcga tcttctcggc
     8281 gcgcgccttg gcttccgtga tgaccgcggc cgccttcgcc tcgagttcgg cgcgccgctc
     8341 ctcgaacgcg gcaatgttct ccggggtagc acgcgtcgcc ttgccctgcg gcaacaggta
     8401 gttgcgcgcg tagcccggct tcaccttgac cttgtcgccg atgccgccga gattctcgac
     8461 cttgtccagc agaatgactt ccatcttcct cgccctcgtt gctccccggc ccgaacccgg
     8521 gaattaagtg atcaaacctt gcctgcggcc ttgcgcctca ggtccatgaa agtgtccgcc
     8581 atgccgtaaa aggcgaagcc gaccaatgtc cacggcgaca ggaacaccag cgccgtccag
     8641 cccgcgaaca gccacgcccg atgcatgccc ttgattcgca ccaggccgtg catcaccgca
     8701 ataccctgca gcagcagcgc cgctcccagc ggcaacgcca ccgggcccgc caccgcctgc
     8761 ccgccggcca gcactccgac ggtcgccagc gcgcccacgc cgcccaccac cggcccgagc
     8821 tgcagggcgc gaaacgcctc gccgaaggcg cccggctgcg ccaggcccgc ccgcagccac
     8881 atggcgatga acatcccgcc cagcgaaacc agcagcagcg acgcggcaat gatgcccggc
     8941 agcagcgcca gcaggcgctc ggccatcagt gccgcgtccg tgcccgtctg cgccgccagc
     9001 ggcgcgacca tctccgtcac cagcccgacc cagggctcgc cctgcgtcgg cgccatcagc
     9061 gaccagccac cggccagcac caccgcgccg gtggtcgcca cgcgggccgc ggcggacagc
     9121 gagccggtgc tgatcaacag ctgcgtcacc gccagcgtgg gcgcccagat cgtcaccgcc
     9181 gaaagcacgc ccgcgaccgg atgcgccccc gacaaagcag ccaccaccgc cagcaaggcg
     9241 gccgcgatcc cgcccgccag ccaggcggac gccgcgcccc gctgcaacgc caccagcgcc
     9301 agcaccgcac agcttgcggg cgccagcagc ggcaacagca cgaggccggc cgccatcaac
     9361 gcggcgcgat agtcgcgttc cgtgatccag cgggcgaact ccagcatgcg gcagccccag
     9421 cacaccctgc gaggggtgcg tgggcgacac cgaccgtcag tgacggtcgg tgtacggcag
     9481 caatgccagg aaacgcgcgc gcttgatggc gaccgcgagc tggcgctggt agttcgagtt
     9541 cgtcccggtg atccggctcg ggacgatctt gcccgtctcg gtcacgtagg ccttcagggt
     9601 ggcgagatcc ttgtagtcga tctcgacgac cttgtctgcc gtgaagcggc agaactttct
     9661 cctgcgaatg aatcgggaca tgttcctgct actcctcagt cctgggacgc gcggcggcgg
     9721 gacggcctgg cgtcttcgtc gcggtcgtcg tcgtccgaat cgtcgtcgtc gtcatcgtca
     9781 cggccgcgtc gcgagcgccc ttcgcgctca tcctccggct tggccatcgg cgacggctcg
     9841 gtgacggcct cgtccctggc gatcaccagg tggcgcagga cggcgtcgtt gaagcggaat
     9901 gcgccggtga gctcgtccag ggtcgccttg ctgcactcga tgttgagcag gacgtaatgg
     9961 gccttgtgga ccttctggat ggggaaggcg agctggcgcc ggccccagtc ttcctggcgg
    10021 tggatggtgc ccccgtcgcc ctcgatcatc ccgcggtagc g
//
LOCUS       DAOMPG010000076        10026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_2902_length_10026_cov_3.446996, whole genome shotgun sequence.
ACCESSION   DAOMPG010000076 DAOMPG010000000
VERSION     DAOMPG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_10026_cov_3.446996"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1140)
                     /locus_tag="PLS34_05770"
     CDS             complement(37..1140)
                     /locus_tag="PLS34_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP87012.1"
                     /translation="MSELALAERYPALAELLPTVPLGAWPTPVQPAEHGALPGPCAGL
                     WLKRDDRCATPYGGNKVRKLEFLLADALRRGHEAVFTFGVAGSNHALATAIYAQQLGL
                     EALLLLTPQSNSSFVGRNLKLGRWAGARQLHCATEAEANRRARILELHGRGTGPAPYR
                     IPGGGSSPLGTVGFVNAGLELAAQVRAGLIPEPDDLYVALGTMGTAAGLALGLAVAGL
                     RTRLVLVRVVRADIASPQRFRSLYHGAARLLHQLDPRFPRVPLDTSRIELRHEFIGPG
                     YARFTAEGMAALAFARQAFDIGLEGTYTGKALAALLADLRAGRLANRHVLFWNTYNGQ
                     PMPAAAMALDYRELPPPFHRYFETPFQPLDPQA"
     gene            complement(1137..1370)
                     /locus_tag="PLS34_05775"
     CDS             complement(1137..1370)
                     /locus_tag="PLS34_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87013.1"
                     /translation="MSSGTGRRGAAPAGQGLRDALRWLSASGRHDAAAVEEACRHFDL
                     SPLDEEFLLAEARRVRAAGKADDDASDQGAPSP"
     gene            1458..2168
                     /locus_tag="PLS34_05780"
     CDS             1458..2168
                     /locus_tag="PLS34_05780"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 family protein"
                     /protein_id="HRP87014.1"
                     /translation="MSGTDHLDALRRDASSAPVVLFASRITDVRELVQRLDEMQVEYR
                     LVTLSMSDSTSRDRFHVLEEWTGHRTLPQVFLDGRFIGGPTELLAHPRLAGGVPAAGQ
                     WLGYTGLIPFVAALLGFLFGDEPRQAYFARQFVAYGAVILSFVGAVHWGVALGSGRLQ
                     IMRLSFSVLPALLAWAALLLPRVPAAWLLLVGFVVLRAWEASPPVASTLPAWYRGMRT
                     RLTLVVGLLVLVFALAAG"
     gene            2226..3821
                     /locus_tag="PLS34_05785"
     CDS             2226..3821
                     /locus_tag="PLS34_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87015.1"
                     /translation="MADPLTPGILVEEFPGRETSLDFIPAGVSAFVGAAPRGPVNQAV
                     HVESLEAFDHCFSVSGGDYPLQRCVQDFFAAGGRRAAIVRVANGARSCTIRLPGREGV
                     LLLEALSPGRREYLRASVDYDHIGPSDDVTFNLVVQRLRAPGTERVADQEIYQRLSLR
                     PSSERYVVDVLLESRLVRVRGLPPPSRPAATVSTAPGYPVTWVEAEDDGADGKALTDY
                     DLVGSSTLGSGLFALGGIGPVDFLCLPPAFDGRKPGPALLLAALRYCRKHRAMLLLEP
                     PAGTYDGEQALAWLRGLNIAGENAMAVFPQLATDARSAGRSAVGAVAGALSRAPADVE
                     PMLGAQFRPVNELAPEARRRLQAAGINVIGRGRGGRIVLQGDRTLASPECPVQLWRSL
                     SARRLALSIERTLLQGTRWVVFEPSGPDLARRLATQLSGWLESLRFAGLLAGDQGDAW
                     FVDVNEVSAPHRPTQVEFTIGFAPRHPREFIIYRVSQGLHGARLALVSPERWAITQPL
                     REFDVGDTDTDAAPALRLGAEAG"
     gene            3818..4345
                     /locus_tag="PLS34_05790"
     CDS             3818..4345
                     /locus_tag="PLS34_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRP87016.1"
                     /translation="MSAADERVVYFAYGANVHTAWLKRRVPDAELIGPGVLPGYRLAF
                     RKRGRDGSARSDACPCDEPGAGLPGALYGMRAADLQRLGAAGAGYLARQVRVESADGT
                     LDAITWVAAPEEIAAGLLPWDWYVALIRNGAAQLGLPEAHRRWLASVRVAVDYDSARA
                     APARQIVAAALSGDA"
     gene            complement(4342..4662)
                     /locus_tag="PLS34_05795"
     CDS             complement(4342..4662)
                     /locus_tag="PLS34_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87017.1"
                     /translation="MEPSKQTVRGRWDPYAVWKRLLRNHPVHDQDPAGSCGRIDRGSF
                     ADFCEAIMDYPDYLQDALLVIAHARGPEFAVHALDDAVREQLFGPSRRLPVAVAPGDA
                     RSQP"
     gene            complement(4707..6994)
                     /locus_tag="PLS34_05800"
                     /pseudo
     CDS             complement(4707..6994)
                     /locus_tag="PLS34_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
     assembly_gap    6995..7004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7005..8319)
                     /locus_tag="PLS34_05805"
     CDS             complement(<7005..8319)
                     /locus_tag="PLS34_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRP87018.1"
                     /translation="MAAGPTQGWHFWIDRGGTFTDIVARRPDGRLETLKLLSEAPDRY
                     PDAAVEGMRRLLDLDDAAALADAPIVAVRMGTTVATNALLERRGEPTLLVITRGLGDV
                     LLIGTQQRPRLFDLDIRLPPPLYDRVVEAEERIAADGEVLQPLNEAALAAELRAARAA
                     GLNAVAIALLHGYRHPAHEQAAARLAREAGFMQVSVSHEVSPLARIVPRGATTVADAY
                     LSPVLGRYVAQLRGPLRRQRLLFMQSNGGLAEADRFRGRDSVLSGPAGGVVGMAAAGR
                     RAGCARLIGFDMGGTSTDVCLYEGEFERSQDNEVAGVRLQAPMLLIHTVAAGGGSVLR
                     FADGRFQVGPESAGAQPGPACYGLGGPATVTDANLALGRLQAAAVPAVFGPGGDRPLD
                     HAAAMARLHELSAAATAAGQSPKRVEAVAAGLLEIAVERMARAVKH"
     gene            8379..9794
                     /locus_tag="PLS34_05810"
     CDS             8379..9794
                     /locus_tag="PLS34_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HRP87019.1"
                     /translation="MSRAEAILYTLVVYKLALVAIGVWATRRTHDAGDFFLGGRRLGG
                     WVAAISASASSSSAWTLVAVSGMAYLWGWPALWLFPATLSGFLINWLWVAPRLMRASR
                     TDGSITLTEFLAGPDAHQGWPRRIVLLASGVVLFSFTFYIAAQFQAAGNAFASAFELE
                     MRTAVMIGAAVVLLYTLLGGFWAVSVTDTLQGLVMALTALAVPVAAVIAAGGPAAVID
                     ALGAQGPGGAPPTAGIAAVGFVLGTLGIGLGYPGQPHVVNRFMALRDERALRDAKVIA
                     LSWAVIIYAGMLTAGFAARALDGGLLAHEQVLFEVTNRLFAPVVAGIMIAAVLSAIMS
                     TADSQLLVAASSVSHDLPRRRRGSLAVSRLVVAVISLLAVAVAVLLPQTIFVRVLFAW
                     TALGAAFGPPLLVRLAGFEVPPARVFAAMATGFLGTVCCYWLPSMPGDWLERLVPFFA
                     SLSICMISARRMSNHERRHAS"
     gene            9822..>10026
                     /locus_tag="PLS34_05815"
     CDS             9822..>10026
                     /locus_tag="PLS34_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87020.1"
                     /translation="MRRDRRIRLLLAAVVIVALVLFAGALLTVADTVLSIVQRLREGP
                     AAIYYGFIALLALGGAAAAWLLWW"
BASE COUNT         1274 a         3555 c         3664 g         1523 t
ORIGIN      
        1 ccggaaaccg gggtctgacc ccggtttcgc gccgggtcag gcttgcggat cgagcggctg
       61 gaacggcgtc tcgaaatagc gatggaacgg cggcggcaat tcgcggtagt cgagcgccat
      121 cgccgcggcc ggcatcggct ggccgttata ggtgttccag aacagcacgt gtcgattcgc
      181 cagccggccg gcgcgcaggt cggccagcag cgccgccagt gccttgccgg tgtaggtgcc
      241 ttccagcccg atgtcgaacg cctgtcgcgc gaaggccagc gccgccatgc cctcagccgt
      301 gaagcgcgca tacccgggcc ctatgaactc gtggcgcagc tcgatgcgag aggtgtcgag
      361 cggcacacgg ggaaaacgcg ggtcgagttg atgcagcagg cgcgcagcgc cgtggtacag
      421 gctgcgaaag cgctgcgggc tggcgatgtc ggcgcgcacc acgcgcacca gcaccagccg
      481 ggtgcgcagc ccggccacgg ccagtcccag cgccaggccg gcggcggtgc ccatggtgcc
      541 gagcgcgacg tagagatcgt ccggttcagg gatcagtccc gcgcgcacct gcgcggcaag
      601 ttccaggccg gcattgacga agccgacggt gccgagcggg gaagaaccgc cgccggggat
      661 gcggtagggg gccggtcccg tgccgcggcc gtgcaactcg aggatgcgcg ccctgcggtt
      721 tgcctcggcc tcggtggcgc aatgcagctg ccgggcgccg gcccagcgtc ccagcttcag
      781 gttgcggccg acaaacgacg agttcgactg cggcgtcagc aacagcagcg cctcgagacc
      841 gagttgctgc gcgtagatcg ccgtggccaa cgcatggttc gagccggcca cgccgaatgt
      901 gaacaccgcc tcgtggcccc ggcgcagcgc atcggcgagc aggaattcca gcttgcgaac
      961 cttgttgccg ccgtagggcg tcgcgcagcg gtcgtcgcgc ttcagccaca gtcccgcgca
     1021 cggcccgggc agcgcgccgt gttcggccgg ctgcacgggg gtcggccaag cgcccagcgg
     1081 cacggtcggc agcagctcgg cgagcgcggg atagcgctcg gccagtgcga gttcgctcac
     1141 ggcgacggcg ctccctggtc ggacgcgtcg tcgtccgcct tgccggcggc gcgcacccgg
     1201 cgcgcctcgg cgagcaggaa ttcctcgtcg agcggggaca ggtcgaagtg ccggcaggcc
     1261 tcctcgaccg cggccgcgtc gtggcgcccg ctggccgaca accagcgcaa tgcgtcgcgc
     1321 aggccctgcc ccgccggagc cgcgccgcgt ctgcccgtgc cggaactcat ctgaactctc
     1381 cctgcctctc gtcgcgtttc gcgatcggct attatcatct caggaaacgc aggccaaaac
     1441 gcgatggcgg caataccatg agcggcaccg accacctcga cgcgctgcgc agggacgcga
     1501 gcagcgcccc ggtcgtgctg ttcgcatcgc gaatcaccga cgtgcgtgag ctcgtgcagc
     1561 ggctcgacga gatgcaggtc gaataccggc tggtgacgct gtcgatgtcg gacagcacct
     1621 cgcgcgaccg cttccacgta ctcgaggaat ggaccggcca ccggacgctc ccgcaggtat
     1681 ttctcgacgg gcgattcatc ggcgggccga ccgagctgct cgcacatccg cgcctcgcgg
     1741 gcggcgtgcc ggcggccgga cagtggctcg gctataccgg gctgattccc ttcgtcgccg
     1801 cgctgctcgg cttcctgttc ggtgacgagc cgcgccaggc ctatttcgcg cgccagttcg
     1861 tcgcctatgg cgcggtgatc ctgtcgttcg tcggggcggt tcactggggt gttgcgctcg
     1921 gctcgggacg actgcagatc atgcgcctgt ccttcagcgt gctgcccgcg ctgctggcgt
     1981 gggcagcact cctgctgccg cgcgtgccgg cggcctggct gctgctggtc ggcttcgtgg
     2041 tgctgcgcgc ctgggaggcc agtccgccgg tggcaagcac gctgcccgca tggtatcggg
     2101 gcatgcgcac tcgcctgacg cttgtcgtcg gcctgctggt gctcgtgttc gcgctggcgg
     2161 cgggatgaac tcgctgatag cctttgtcct gcacatcgtg tcccagcgcg ccgccggagt
     2221 aatgcatggc tgatccgctc actcccggga ttctcgtcga ggaatttccc ggccgtgaga
     2281 catcgctgga cttcatcccg gccggggtga gcgccttcgt cggcgccgcg ccgcgcggtc
     2341 ccgtcaacca ggccgtgcac gtcgagagcc tggaggcatt cgatcattgc ttctcggtca
     2401 gcggcggcga ttatccgttg cagcgttgcg tgcaggattt cttcgcggcc gggggacggc
     2461 gcgcggcgat cgtgcgggtc gcgaatggcg cgcgctcctg caccatccgc ctgccgggcc
     2521 gcgaaggcgt gctgctgctc gaggcgctca gtccgggacg acgggagtac ctgcgcgcct
     2581 cggtcgacta cgatcacatc ggtccgtccg acgacgtcac cttcaacctc gtggtgcaac
     2641 gcttgcgtgc gccgggtacc gagcgcgtcg ccgaccagga gatttaccag cggctttcgc
     2701 tgcggccttc ctcggagcgc tacgtcgtcg acgtcctgct ggagtcgcgc ctggtgcgcg
     2761 tgcgcggcct gccgcctccc agcaggccgg cggccacggt gagcaccgcg cccggttacc
     2821 cggtgacctg ggtggaggcg gaagacgacg gcgccgacgg caaggcgctg accgactacg
     2881 acctggtcgg ctcttccacg ctcggcagcg gcctgttcgc gctcggcggc atcggcccgg
     2941 tcgatttcct ctgcctgccg ccggcgttcg acggacgcaa gcccgggccg gcgctgttgc
     3001 tggccgcact gcgctactgt cgcaagcatc gcgcgatgct gttgctcgag ccgccggccg
     3061 gcacctacga cggcgagcag gcgcttgcgt ggttgcgcgg actgaacatc gccggcgaga
     3121 acgccatggc ggtgttcccg cagctcgcca cggatgcgcg aagtgccggg cggtcggcgg
     3181 tcggcgcggt cgccggcgcc ctgtcgcgcg cgccggccga cgtcgagccc atgctcggcg
     3241 cgcagttccg cccggtcaac gagctggcgc cggaagcgcg tcgccgcttg caggccgccg
     3301 gcatcaacgt catcggccgc ggtcgcggcg gccgcatcgt gttgcagggc gatcgcacgc
     3361 tggcgtcgcc cgagtgcccg gtgcagctgt ggcgatcgct gtcggcgcgc cggctggcct
     3421 tgagcatcga gcgcaccctg ctgcagggca cgcgctgggt agtgttcgag ccgtcgggcc
     3481 cggacctcgc ccggcggctg gcgacgcagc tcagcggctg gctcgagtcg ttgcgctttg
     3541 ccgggctgct ggcgggcgac cagggcgacg cctggttcgt agacgtcaac gaagtctcgg
     3601 caccgcaccg gcccacgcag gtcgagttca ccatcgggtt tgcgccgcgg cacccgcgcg
     3661 agttcatcat ttaccgggtg agccagggcc tgcacggcgc gcgcctggcg ctggtctcgc
     3721 cggaacgctg ggccatcacc cagccgttgc gcgagttcga cgtcggcgac accgacaccg
     3781 acgccgcgcc ggcgctgcgc ctcggtgccg aggccgggtg agcgccgccg acgagcgcgt
     3841 cgtctatttt gcttacggcg ccaacgttca taccgcctgg ctcaagcgcc gcgtccccga
     3901 tgccgagctg atcggccctg gcgtgctgcc gggttaccgg ctggcgtttc gcaagcgggg
     3961 tcgcgacggg tcggcgcgct cggacgcctg cccgtgcgac gaacccggtg ccgggctgcc
     4021 gggcgccctg tacggcatgc gcgcagcgga cctgcagcgg ctcggtgcgg ccggcgccgg
     4081 ctacctggcg cggcaggtcc gggtcgagtc cgccgacggg acgctggacg cgatcacctg
     4141 ggtcgcggca ccagaagaga tcgctgcggg gttgctgccg tgggactggt acgtggcgct
     4201 gattcgcaac ggcgccgcgc agctcggctt gccggaggcg catcggcgct ggctggcgag
     4261 cgtccgggtc gcggttgatt acgattcggc gcgcgccgcg ccggcccggc agatcgtcgc
     4321 cgccgcgctg tccggggatg cctagggctg cgaacgtgcg tcgcccggcg ccacggccac
     4381 cggcagccgc cggctcggcc cgaagagctg ctcgcgcacc gcgtcgtcga gggcgtgcac
     4441 ggcgaattcc gggcccctgg catgcgcgat caccagcagg gcgtcctgca ggtaatccgg
     4501 gtaatccatg atcgcttcgc agaagtcggc aaagctgccg cggtcgatgc ggccgcaaga
     4561 gcccgcagga tcctggtcgt gcaccggatg gttgcgcagc aggcgtttcc acactgcgta
     4621 ggggtcccag cgcccgcgaa cggtttgctt cgatggctcc acgaactcac gctagcaaag
     4681 ttgtgtgcag cggaccgtga gcgaggtcac gactgcgacc caaaaccgcc gccaccgggc
     4741 gtcgcgatct ccagcgcgtc gcccggctgc aggcgaaccg aggctattcc ctcgagctcc
     4801 tcgatcgtgc cgtcggcgcg gattacgcgg ttgcggccgg gcaggcccgg tgcgccgccg
     4861 gccaagccga agggcggcac gcgccggtgg ttggaaatca gcgcggcgtc cagcgctgcg
     4921 gtgaacagca ggcggcggac gacgccgtcg ccgccccgat gccgtcctgc gccgccggag
     4981 gcgtggcgta cccggaagga ctcgacgcgc accgggtggc gcagctcgag gatctcggga
     5041 tccgtaaggc ggctgttggt catgtgggtg tgcaccgccg aggcgccgtc gaacgcgggg
     5101 cccgcgccgg cgccgccgca cagcgtctcg tagtactggt gccgggcgtt gccgaagctg
     5161 aggttgttca tggtgccctg cgcggctgcg accacgccga gcgcgccgaa cagcgcgtcc
     5221 accacgcatt gcgaggtttc gacgttgccg ccggccaccg cgccaggcca gcgcgggttc
     5281 agcaggctgc cggtcgggat gcgcagctcc agcggtgcga ggcagccctc gttcagcgga
     5341 atgtcgcggt ccaccaggca gcgaaaggcg tacagcacgg cggcgcgaca gacggaggcc
     5401 ggtgcattga gattgcccgg ggactgcgcg gaggtgccgg tgaagtcgat caccgcgcgg
     5461 cgcgcggcgc ggtcgatgcg gacggcgacc tggaccgtct cgccgccgtc cagctgccag
     5521 cggaattcgc cgtccgccag ccgcaacagg gcatcgccca ccgcttcggc cgcgttgcgc
     5581 tggatgtggc ccatgtaggc cagcaccgcg tcggcgccga actcgctcat catgcgctcg
     5641 agctcgccga tgccgcgccg gttggcggcc agctgcgcgc ccaggtcggc caggttctgc
     5701 gccgggttgc gtgccggcca gcggccgccc tcgagcagcg tgcgcatgcc agcctcgtcg
     5761 aagcgtcccg cgctgaccag ccgcaggccg cggatcagcg tgccttcctc gtcgatgtgc
     5821 gtgctgtggg ccggcatcga gcccggcgtg cagccaccga tgtcggcgtg gtgcgctcgc
     5881 gatgcgacgt acagttgtgg tcggtcggca gcgccgaaaa acggcgtcac gacggtgatg
     5941 tcgggcaggt gcgtgccgcc ctcgtaggga tcgttgagga cgaagctgtc gcccggcgtc
     6001 atgccgtcac cgaagcgcgc catgacggcg cgtacgctct cgcccatcga gcccaggtgc
     6061 accggcatgt gcggcgcgtt cgcgaccagc ccgccggccg ggtcgaacac cgcgcaggag
     6121 taatcgagcc gctccttgat gttcacggaa ctggcggtgt tctgcagcac cgcgcccatc
     6181 tgctcggcga cgtgcatgaa gcggttgttg aacacttcca gcagcaccgg gtcggaacgg
     6241 gtgggcgcgc ggcgcacctg ccgccgctgc ggccggcata gccgcaggtt gcccagcgca
     6301 tcgagctccg cgcgccagcc gggtgcgacc caggtcgtgg cattggcttc cggcaccagg
     6361 gccgggccgt cgagccgctc gcccgggcgc agcgtggcac gcggcaccag cgggatctcc
     6421 cgccaggcat cgcccaccca ggcgaggccg gtggtgcggg ccgtggcggc gcgggtcggt
     6481 tcggcgagca cgccggtctc gtcaaccgcg ccttcgcccg cagcgccccc gccaccgcgc
     6541 gcctcggctt ccatggattc cagcatcacg cgatgtgcgg ccacgaagcc gaagcgtcgc
     6601 tcgtgggccg cggcgaagcc ggcgcgcact tccgcctcat tggccagcgg cacttccagc
     6661 gtcgtatcgc tgcccgcgta gcgcaggcgc aggcgatgct cgacggcaat ggcctcgcgc
     6721 ggcacgccct gcgacacgag cgccacggcc gccgcctcgc ccagctcgag cagcgtgcgc
     6781 gcggcggccg ccagcgtgtc gccgtccagc gccgcctgca cgggcgcctg gcgtagttcg
     6841 accaggtcgg cgaggccgat gccccaggcg gacagcacgc cggcgaaggg cggcagcagc
     6901 acgtcacgaa tgtccagcgc atcggcgacg tcgcaggcat gctggccggc ggcgccgccg
     6961 aagccgcaca gcgtgaacgc cgcgggatcg gcgcnnnnnn nnnncgtgct tgacggcgcg
     7021 cgccatgcgt tccacggcga tctccagcag tccggcggcg accgcttcca cgcgcttcgg
     7081 cgactgcccg gcggcggtcg ccgcggcgct gagctcgtgc agccgcgcca tggcggcggc
     7141 atggtcgagc ggccggtcgc cgccgggccc gaacaccgcc ggcacggccg ctgcctgcaa
     7201 ccggccgagc gccaggttgg cgtcggtgac ggtcgccggc ccgcccagtc cgtagcaggc
     7261 cgggcccggc tgcgccccgg ctgattccgg gccgacctgg aagcgcccgt cggcaaagcg
     7321 cagcaccgag ccgccgcccg cggcgaccgt gtggatcagc agcatcggtg cctgcaggcg
     7381 gacgcccgcg acctcgttgt cctggctgcg ctcgaactcg ccctcgtaga ggcagacgtc
     7441 ggtggaggtg ccgcccatgt cgaaaccgat cagccgcgcg cagcccgcgc ggcggccagc
     7501 cgcggccatg cccaccacgc cgccggccgg ccccgacagc acgctgtcgc gaccgcggaa
     7561 gcggtccgcc tccgccagcc cgccgttgct ctgcatgaac agcagccgct ggcgccgcag
     7621 cggtccccgc agctgcgcga cgtagcggcc cagcaccggc gacaggtagg cgtccgccac
     7681 cgtggtcgcg ccgcgcggca cgatgcgcgc cagcgggctg acctcgtgcg acaccgagac
     7741 ctgcatgaag ccggcctcgc gcgccagccg tgccgccgcc tgctcgtgcg ccggatggcg
     7801 atagccgtgc agcagcgcga tggccacggc attgagcccg gccgcgcgcg cggcacgcag
     7861 ctcggcggcc agcgccgctt cgttgagcgg ctgcagcacc tcgccgtcgg ccgcaatgcg
     7921 ttcctcggcc tcgacgacgc ggtcgtagag cgggggcggc aggcggatgt cgaggtcgaa
     7981 cagccgtggc cgctgctgcg tgccgatcag cagcacatcg ccgaggcccc gcgtgatgac
     8041 cagcagggtg ggctcgccac ggcgctcgag cagagcgttg gtggccaccg tcgtgcccat
     8101 gcgcaccgcc acgatcggcg catcggcaag cgccgccgca tcgtcgaggt cgagcaggcg
     8161 ccgcatgccc tcgacggcgg catccgggta acggtcgggc gcttcggaca gcagcttgag
     8221 cgtctcgagc cgcccgtcgg ggcggcgcgc cacgatgtcg gtgaaggtgc cgccccggtc
     8281 gatccagaag tgccagccct gcgtgggtcc cgctgccatg cgccgattgt acggcgactg
     8341 cggctgctac actgccgcgg tcgctggcgg ggggcgcgat gagccgggcc gaagccattc
     8401 tctacacgct ggtcgtctac aagctggcgc tggttgcgat cggcgtctgg gcgacgcgcc
     8461 gcacccatga tgccggcgac ttctttctcg gcggccgccg cctcggcggc tgggtcgccg
     8521 cgatcagcgc ttctgcctcc tcgtcgtcgg cctggacgct ggtggcggtc agcggcatgg
     8581 cctacctgtg gggctggccg gcgctgtggc tgtttcccgc caccctgtcg gggttcctga
     8641 tcaactggct gtgggtcgcg ccgcggctga tgcgcgccag ccgcaccgac ggttccatca
     8701 cgctgaccga gttcctcgcc gggcccgacg cacaccaggg ctggccgcgg cgcatcgtgt
     8761 tgctggcctc cggggtggtg ctgttttcct tcaccttcta catcgccgcc cagttccagg
     8821 cggccggcaa cgccttcgcc agcgccttcg agctggagat gcgcacggcg gtgatgatcg
     8881 gcgccgcggt ggtcctgctg tacacgctgc tgggcggatt ctgggcggtg agcgtcacgg
     8941 atacgctgca gggactggtg atggcgctga ccgcgctggc ggtgccggtg gcggcggtca
     9001 tagcggccgg cggcccggca gcggtgatcg acgcgctcgg cgcgcaggga ccgggcggcg
     9061 cgccgccgac ggcgggcatc gccgcggtcg gtttcgtgct cggcacgctc ggcatcggcc
     9121 tcggctaccc gggccagccg cacgtggtca atcgcttcat ggccctgcgc gacgaacggg
     9181 cgctgcgcga cgccaaggtc atcgcactgt cctgggcggt catcatctat gcgggcatgc
     9241 tgaccgccgg ctttgccgcg cgtgctctcg acggcgggct gctggcgcac gagcaggtgc
     9301 tgttcgaggt caccaaccgg ctgttcgcgc cagtggtcgc ggggatcatg atcgccgcgg
     9361 tgctgtcggc catcatgtcg acggccgaca gccagttgct ggtggccgcc tcatccgtga
     9421 gccatgacct gccgcgccgc cgccgcggca gcctcgccgt gtcgcggctg gtcgtggccg
     9481 tgatcagcct gctggcggtg gcggtggcgg tgctgttgcc gcagaccatt ttcgtgcggg
     9541 tgctgttcgc ctggaccgcg ctcggcgcgg ccttcgggcc cccgctgctg gtgcggctcg
     9601 ccggattcga agtgccgccg gcgcgcgtgt tcgcggccat ggccaccggc ttcctcggca
     9661 cggtgtgttg ctactggctg ccgagcatgc cgggagactg gctggagcgg ctggtgccgt
     9721 ttttcgcttc gctttcgatc tgcatgatct ctgcgcgtag gatgagcaac catgaacgcc
     9781 ggcatgcaag ctgacaggtc gggccgggcg gcgccgcgag gatgagacgc gaccggcgca
     9841 ttcgcctgct gctggccgca gtggtcatcg tggccctggt gctgttcgcc ggcgcactgc
     9901 tgacggtggc ggacaccgtg ctgtcgatcg tgcagcgcct gcgcgagggc ccggcggcaa
     9961 tctactacgg tttcatcgcg ctgctcgcat tgggcggcgc ggcggccgcc tggctgttgt
    10021 ggtggc
//
LOCUS       DAOMPG010000077         9676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3036_length_9676_cov_5.184492, whole genome shotgun sequence.
ACCESSION   DAOMPG010000077 DAOMPG010000000
VERSION     DAOMPG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3036_length_9676_cov_5.184492"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLS34_05820"
     CDS             <1..330
                     /locus_tag="PLS34_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87021.1"
                     /translation="EIERPARSSRRERTEWGSRPPEGRGGPGGGRGGRSGRSGGRSSG
                     GSGRASGGPRREERPARPALETPPAEAAAPVGVEGGGDPAKKRRRRRKRKSAGDGAPG
                     GGAPAAE"
     gene            complement(349..1044)
                     /gene="yrfG"
                     /locus_tag="PLS34_05825"
     CDS             complement(349..1044)
                     /gene="yrfG"
                     /locus_tag="PLS34_05825"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HRP87022.1"
                     /translation="MELVSRHPFEWDHYDTVLLDMDGTLLDLRFDNFFWQELIPARYA
                     ALHNLPHEEAVAVLEPRFAAARGTLEWYCLDHWSRELGLDVAALKREAEDHIDFLPDV
                     PDFLLAVRELRKRVVLVTNAHRGSLSIKLERTGLARYLDAIHSSHDLGVPKEHAEFWG
                     RLRAIEPFDAPRTMLVDDSLPVLRSAHDFGIGRVIAILRPDSSRPAVAAADFHGVESV
                     TELLPRGPRRGGG"
     gene            1086..1886
                     /gene="cysQ"
                     /locus_tag="PLS34_05830"
     CDS             1086..1886
                     /gene="cysQ"
                     /locus_tag="PLS34_05830"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRP87023.1"
                     /translation="MTELAKDLLEDVARIAVAAGRAILEVYETDFGVEKKADASPITE
                     ADRRAHDIIRAGLAGLTDQRPMLSEEAHTVPWEVRRHWKEYWLVDPLDGTKEFIQRNG
                     QFTVNIALVRAGRPVLGVVHVPVSGVTYGGIVGRGAWRQTRGGPRDPIRVRPLGDGPV
                     RVVGSRSHRGPDLDGYLERLGPHEMVPMGSSLKFCVVAEGNADVYPRLGPTSEWDTAA
                     AEAVLWAAGGKVVANDGEPMDYNKGPELLNPWFIAAGDDSRDWTRYLP"
     gene            1883..2236
                     /locus_tag="PLS34_05835"
     CDS             1883..2236
                     /locus_tag="PLS34_05835"
                     /inference="COORDINATES: protein motif:HMM:NF023171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HRP87024.1"
                     /translation="MTLQAMMVVAGAVLAVIWFWHASMAAREHANSAAREACDRLRLV
                     MLDGTVAISRLWPRRDADGRLRLERTYGFEYTDDGHRRLRGFVVTLGPRVISVGLASA
                     TSVGPPARTPPDVPD"
     gene            2337..2555
                     /locus_tag="PLS34_05840"
     CDS             2337..2555
                     /locus_tag="PLS34_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRP87025.1"
                     /translation="MKDNKVDTYIFSNMTRLKELRIAHRDLDDVITRLNENPLVDQLQ
                     MKRLKKRKLMLKDQIARLESELIPDLNA"
     gene            complement(2613..4967)
                     /locus_tag="PLS34_05845"
     CDS             complement(2613..4967)
                     /locus_tag="PLS34_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018981755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP87026.1"
                     /translation="MSEPKPARITVALENLIFRNRPLVIALFALVTVLLAWSAVTGLR
                     VDAGFSKQLPLEHEYMETFLEHYEDFGGADRILIALMDESGDMFNPEFFKALAGATDA
                     VFFIPGVDRARVTSIFTPNVRYLEVTEEGIEAGDVIPADFQPTPSGLERVKENILKAG
                     IVGRLVANDFSGAIVSAQLLEINPDTQERLDYFDVARLLETEVRDKFQTDQIKVRIIG
                     FAKVVGDIKEGANRIVLFFGVALFITAILVFIYSQSLKLTIVPLVCSIFAVIWELGAL
                     PLLGFGIDPMGLLVPFLVFAIGVSHGVQMISGFAAETYDGADPETAARTTFRRLLVPG
                     GIALLSDTIGFITILFIDIRIIQEMAITASLGVACIILTNLVLLPVLISYVHPGPKYK
                     EKLLNRAEQMRPLWEGLSKVSTRGPATVIIVIAAGLFVFGAWKGSQVRIGDLLAGVPE
                     LRPDSRYNLDSQAVTDHFSIGVDLINVIVEVGENGCIKYEVMETIDQFAWHMENVPGV
                     QTVVALPQVAKIVTAGWNEGFPAWRVLSRNEQVLVQALARVETSTGLLNRDCSVIPVM
                     VFTEDHKAETIERVVNEVKAWRERFAIEGTEFRLATGNVGVMAAMNEEVAASQFPILI
                     YVFGAVILLCWASFGSLRAVLCIVIPLALVSLLAYALMAYLNIGLKVNTLPVAALGVG
                     IGVDYGIYIYSRFQYFLRQGHSIQNAYNMTLRVTGNAVVFTGITLAIGVFTWVFSPLQ
                     FQADMGILLTFMFLVNMFGAILLLPALATWLLPAKYQHERPEEA"
     gene            complement(4971..5990)
                     /locus_tag="PLS34_05850"
     CDS             complement(4971..5990)
                     /locus_tag="PLS34_05850"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRP87027.1"
                     /translation="MPESEWSLIMPLASKSLTLDIARVGDTYVVVGDRGHVLISEDQG
                     RSWMQQRTPTRVPLTGVWFHDRNLGWAVGHDAVILRTEDGGESWCRVHFAPELERPLF
                     DVWFADASNGIAVGAYGYLLRSSDGGLTWTEETLDIIEDGAGDETSGEDEYAEDDEFG
                     DEEYADDDGAWDDDSWTDDGVAADVHLNRIFRDAAGRLYIAAEAGSILRSDDQGQTWR
                     ALSPPYDGSFFNGLAPDDDSLLVFGLRGNMFRTWDQGVSWRDVNLPVDTSLFGGTRLV
                     DGGIVVVGTAGVMLVSREGERFRLVQRPDRKALMAAQATDDGGVIVIGEPGIERLERR
                     NLAGE"
     gene            complement(6151..7530)
                     /locus_tag="PLS34_05855"
     CDS             complement(6151..7530)
                     /locus_tag="PLS34_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008484962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HRP87028.1"
                     /translation="MTNLRLLKALAALALVGLAAPASAERLSAEQIARLDADLTPMGA
                     IRAGNEAGTIPAWTGGLRTPAEAGFPNFVSGGHHPDPYADDKPLYRITAANMAQYDDI
                     LTEGHKAMFRTYPEYFMDVYPTRRSAAVPERIYEATKRIAGSTHLIESGNGFSGGAEG
                     IPFPIPENGLHAIWNHIARYRTDSARRTIGQAPVTRDGNYTMVMFTDEYLGAYGLAGA
                     REEDLDNVIIFFKQTVTAPSRLAGEVLLVHETLNQARENRKAWLYNPGQRRVRRAPNV
                     AHDNPRTASDGLATSDQLDIFNGSPERYNWTLVGRKEILVPYNSYKLHSNKLKYSDIL
                     KPLHINQEHARYELHRVWVVDATLKEGTRHVYKRRTFYIDEDSWQAMVVDCYDNRDQI
                     WRVQEAHSINYYDVPSFWTTLELTMDLQSGRYLGIGLNNEERGTYDFSVKYTPSDFSP
                     ASLRRAGVR"
     gene            complement(7552..9540)
                     /locus_tag="PLS34_05860"
     CDS             complement(7552..9540)
                     /locus_tag="PLS34_05860"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HRP87029.1"
                     /translation="MPIQTDNWTGSWDTTLSYGAIWRVQSPDCRLIANANGGCGRSAN
                     NDDGNLNFDTGLVSSAIKATSEIELRFRDSWGLFARGTAFYDGEAGNTNRTEISEGGL
                     TRVEQGGKMLDYFVYVPFNLGSMPAEFRIGNQVISWGESTFIQGGANLTNPVDVGKLR
                     VPGSELKEALVPQGMAYFNVSPTDKFSIEAYYQYDWQNVVPDPVGSYFSTNDLAGKGA
                     TKAMLGFGAWSDLGTDWTPLGGSFDSDFVFVPRGASAAPDDGGQYGLALRYFADSIMG
                     GMEFGFYYLRYHSRLPVVSGLTGTQAGFGNAAAVATAAQAAAIGLASGLSFDAAVNTA
                     TAQGMAAAANLGGDVAQARVRERATVAANTFLSLGSTGVAGLANAFAQDELAQTSRYF
                     LEYPEDLDLYGVSFSGYLFNTGIAWQGEVSYKPDSPMQVDDVELLFAALSPLDNLSPT
                     AGSYVCPTNPNARGNLGCFNQIGVFGLNTVIPGYVELDIWQAQTTFTYLSGPLLGAGT
                     GAFVLELGMNYVPDLPDTNSGGPNNRGLRLNGEGTSLSGNVPLSAAHFFELDPRSAFP
                     HKTSWGYRFRGALVYNNAIGPWSLSPIVGWSHDVNGNSPGPGGNFIEDRTALSVALKG
                     TLRNKYELELNYAQFGGAGIYNTGHDRDFISFTAKVSF"
BASE COUNT         1605 a         3184 c         3337 g         1550 t
ORIGIN      
        1 gagatcgagc ggccggcgcg cagcagccgc cgggaacgca ccgagtgggg ctcacgcccg
       61 cccgaaggac gcggcggtcc gggcggcggg cgcggcggcc gcagcgggcg gagcggcggt
      121 cgcagttcgg gcggcagcgg ccgcgcaagc ggcggaccgc ggcgcgagga gcggccggcc
      181 cggccggcac tggagactcc gccggccgag gcagcggcgc cggtgggtgt cgagggcggc
      241 ggcgatccgg cgaagaaacg gcgccgccgg cgcaagcgca agtccgccgg ggacggcgca
      301 ccgggcggcg gtgcgccggc cgcggaatag cccgcgccgg aacgcgattc agccgccgcc
      361 gcgacgcggg ccccgcggca gcaactcggt cacgctttcc acgccgtgga agtctgcggc
      421 ggcgaccgcc ggccgggagg aatccgggcg caggatggcg atcacgcgcc cgatgccgaa
      481 gtcgtgtgcc gagcgcagca ccggcaggct gtcgtccacc agcatggtgc gcggcgcatc
      541 gaagggctca atggcgcgca gccggcccca gaactcggcg tgctccttcg gcacgccgag
      601 gtcgtggctg gaatggatcg cgtccaggta acgggccagg ccggtgcgtt ccagcttgat
      661 ggacagcgag ccccggtgcg cgttggtgac cagcacgact cgcttgcgca gctcccgcac
      721 ggcgagcagg aagtccggca cgtccggcag gaagtcgatg tggtcctcgg cctcacgctt
      781 gagcgccgcc acgtcgaggc cgagttcccg gctccagtgg tccaggcagt accattccag
      841 ggtgccgcgg gcggcggcga agcgcggctc gagcaccgcc accgcctcct cgtgcggcag
      901 gttgtgcagc gcagcataac gcgccgggat cagctcctgc cagaaaaagt tgtcgaagcg
      961 caggtcgagc agcgtgccgt ccatgtccag caggacggtg tcgtagtgat cccactcgaa
     1021 gggatgccgg gagaccagtt ccatggcccg gtatcatata cgccccgacc cgatggagcc
     1081 ccaccgtgac cgagcttgcc aaggacctgc tggaggatgt cgcaaggatt gccgtggcgg
     1141 ccggccgggc gattctcgaa gtctatgaga cggacttcgg cgtcgagaag aaggccgacg
     1201 catcgccaat caccgaggcc gaccgacgcg cccacgacat catccgcgcc gggctcgccg
     1261 gcctgaccga ccagcggccg atgctgtcgg aggaggcgca taccgttccc tgggaggtgc
     1321 gccggcactg gaaggaatac tggctggtgg acccgctcga cggcaccaag gaattcatcc
     1381 agcgcaacgg ccagttcacc gtgaacatcg cgctggtgcg tgccggccgg ccggtgctcg
     1441 gggtggtgca cgtgccggtc agcggggtca cctacggcgg catcgttggt cggggcgcct
     1501 ggcggcaaac gcggggcgga cccagggacc ccattcgcgt tcggccgctg ggcgacggcc
     1561 cggtcagggt ggtcgggtcg cgctcccacc gcggccccga cctggacggc tacctggagc
     1621 gactcgggcc gcacgagatg gtgccgatgg gctcctcgct caagttctgc gtggtggcgg
     1681 aaggcaatgc cgacgtctac ccgcggctgg gcccgacttc ggagtgggac actgccgcgg
     1741 cagaagccgt gctgtgggct gccgggggca aagtggtcgc caacgacggc gagccgatgg
     1801 actacaacaa ggggcccgag ctgctcaacc cctggttcat cgccgcgggc gacgattcgc
     1861 gcgactggac ccgctacctg ccgtgaccct gcaagccatg atggtggtcg ccggagcggt
     1921 gctggccgtg atctggttct ggcacgccag catggcggcc cgcgagcacg ccaacagcgc
     1981 cgcccgcgaa gcctgcgacc gcctcaggct ggtgatgctc gacggcaccg tcgccatctc
     2041 ccgcctgtgg ccacggcgcg acgccgacgg ccgcctgcgg ctcgagcgca cttacggctt
     2101 cgaatacacc gacgacggcc atcggcgctt gcggggcttc gtggtgacgc tcggaccgcg
     2161 cgtgatctcg gtcggcctgg cgtcggctac cagcgtcggc ccgccggccc gcacgccgcc
     2221 ggacgtcccc gactgaacgc ggccgccggc gcgcccggcc ggtgctggcg gatgcggtgg
     2281 cagttggcta agcttggcgg gtgcggcgcg agggctgaat tccaatggcg acccagatga
     2341 aagacaacaa ggtcgacacc tacatcttca gcaacatgac ccggctcaag gagctgcgga
     2401 ttgcccaccg ggacctcgac gacgtcatca cccggctcaa tgagaacccg ctggtcgacc
     2461 agctgcagat gaagcgcctg aagaagcgca agctgatgct caaggaccag atcgcccgcc
     2521 tcgagagcga gctgattccc gacctgaacg cctaggcgaa ttggggacat tccccatttc
     2581 cgtcaggaaa tggggaatgt ccccaattcg ggtcaggctt cttccgggcg ctcgtgctgg
     2641 tacttggccg gcagcagcca ggtggccagc gccggcagca gcaggatcgc gccgaacatg
     2701 ttcaccagga acatgaaggt cagcaggatg cccatgtccg cctggaactg cagcggcgag
     2761 aacacccagg tgaacacgcc gatggccagc gtgatgccgg tgaacaccac ggcgttgccg
     2821 gtcacccgca gcgtcatgtt gtacgcattc tggatgctgt gaccctggcg caggaagtac
     2881 tggaagcggc tgtagatgta aatgccgtag tccacgccga tgccgacgcc gagcgccgcc
     2941 accggcaggg tgttcacctt caggccgatg ttcaggtaag ccatgagcgc gtaggccagc
     3001 agcgagacca gcgccagcgg gatgacgatg cacagcaccg cgcgcaggga gccgaagctg
     3061 gcccagcaca gcaggatgac ggcaccgaac acatagatga ggatcgggaa ctgcgaggcg
     3121 gcgacttcct cgttcatggc cgccatcacg cccacgttgc cggtcgccag ccggaactca
     3181 gtcccctcga tggcgaaccg ctcgcgccac gccttgacct cgttgaccac cctctcgatg
     3241 gtctcggcct tgtgatcctc ggtgaacacc atcaccggga tgacgctgca atcgcggttc
     3301 agcagcccgg tgctggtctc gaccctggcc agcgcctgca ccagcacctg ctcgttgcgt
     3361 gacagcaccc gccaggccgg aaagccttcg ttccagccgg cggtgacgat cttggccacc
     3421 tgcggcagcg ccaccacggt ctgcactccg ggcacgttct ccatgtgcca ggcgaactgg
     3481 tcgatggtct ccatgacctc gtacttgatg cagccgttct cgcccacctc gacgatgacg
     3541 ttgatcaggt ccacgccgat cgagaagtgg tcggtgacgg cctgggagtc caggttgtag
     3601 cgggaatctg gacgcagctc cggcacgccc gccagcaggt cgccgatccg cacctgcgaa
     3661 cccttccacg cgccgaatac gaacagtccg gccgcaatga cgatgatgac ggtcgcgggg
     3721 ccgcgggtgg acaccttcga gagcccctcc cacagcgggc gcatctgctc ggcgcgattg
     3781 agcagcttct ccttgtactt cggacccggg tgcacatagc tgatcagcac cggcaacagc
     3841 accaggttgg tgaggatgat gcaggccacg cccagcgagg cggtgatcgc catttcctgg
     3901 atgatgcgga tgtcgatgaa caggatggtg atgaaaccga tagtgtccga gagcagcgcg
     3961 atgccgcccg gcacgagcag ccggcgaaag gtcgtgcgcg cggccgtctc ggggtccgcc
     4021 ccgtcgtaag tctcggcggc gaagcccgag atcatctgca cgccgtgcga cacgccgatt
     4081 gcgaatacca ggaacggcac cagcagtccc atcgggtcga tgccgaagcc cagcagcggc
     4141 agggcgccga gttcccagat gaccgcgaag atgctgcata ccaggggcac gatcgtgagc
     4201 ttcagcgact ggctgtagat gaacaccagg atggcggtga tgaacagcgc cacgccgaag
     4261 aacagcacga tgcggttggc accctccttg atgtcgccga ccaccttggc gaagccgatg
     4321 atgcggacct tgatctggtc ggtctggaac ttgtcgcgga cctccgtctc gagcagtcgc
     4381 gcgacgtcga aatagtcgag ccgctcctgg gtgtcgggat tgatctccag cagctgggcg
     4441 ctgacgatgg cgcccgagaa atcgttcgcc accaggcggc cgacgatgcc ggccttgagg
     4501 atattctcct tgacccgctc caggcctgac ggggtcggct ggaaatcggc cgggatgacg
     4561 tcgcccgcct cgatgccttc ctcggtcacc tcgagatagc gcacattcgg cgtaaagatg
     4621 gaggtcacgc gcgcccggtc gacgcccgga atgaagaaca cggcgtccgt cgccccggcc
     4681 agcgccttga agaactccgg gttgaacatg tcgccgctct cgtccatgag ggcgatcagg
     4741 atccggtcag cgccgccgaa gtcctcgtag tgctcgagga aggtctccat gtactcgtgc
     4801 tcgaggggca gctgcttgct gaacccggcg tcgacgcgca ggccggtcac ggcgctccag
     4861 gccagcagca cggtgaccag cgcgaacagc gcgatcacca gcgggcgatt gcggaagatc
     4921 aggttctcga gcgctacggt gatgcgcgcg ggcttcggtt ccgacatcgt ctactccccc
     4981 gccaggttgc gccgctcgag gcgctcgatg ccgggctcgc cgatcactat gaccccgccg
     5041 tcgtcggtag cctgcgccgc catcagcgcc ttgcggtccg ggcgctgcac gagccggaag
     5101 cgttcgccct cgcggctgac cagcatcacg ccagcggtcc ccacgacgac gatgccgccg
     5161 tccacgagtc gcgtgccgcc gaacagcgag gtgtcgaccg gcaggttgac atcgcgccag
     5221 ctgactccct ggtcccaggt gcggaacatg ttgccgcgca acccgaaaac cagcagagaa
     5281 tcgtcgtccg gcgccagtcc gttgaagaac gagccgtcat agggcgggct gagcgcccgc
     5341 caggtctgcc cctggtcgtc ggagcgcagg atcgacccgg cctccgccgc gatgtacagc
     5401 cggccggcgg catcgcggaa gatgcgattg aggtggacgt ccgcagccac gccgtcgtcc
     5461 gtccaggagt cgtcgtccca ggcgccgtcg tcgtcagcgt attcttcgtc gccgaactcg
     5521 tcgtcttcgg cgtactcgtc ctcacccgag gtctcgtcgc cggcgccgtc ttcgatgatg
     5581 tcgagggtct cctcggtcca cgtcaggccg ccgtccgagc tgcgcaacag gtaaccgtag
     5641 gcgccgaccg cgataccgtt gctggcatcc gcgaaccaga cgtcgaacag cgggcgctcg
     5701 agttccgggg cgaagtgcac gcggcaccag ctctcgccgc cgtcctcggt gcgcaggatc
     5761 accgcgtcgt ggccgaccgc ccagcccagg ttgcggtcat gaaaccagac gccggtgagc
     5821 ggcacccgcg tgggagtgcg ctgctgcatc cagctccgtc cctggtcctc gctgatgagc
     5881 acgtgcccgc ggtcgccgac cacgacgtag gtgtcaccga cccgcgcgat atcgagcgtc
     5941 agcgacttcg acgcgagcgg catgatgagc gaccactcgc tctcgggcac gggctcggcg
     6001 cacgttgcaa ccgcagcgct tggtgtgccg gcgagcaggg caagcaagag taggacaagg
     6061 agtctgggca cggctgcagc gcctcgcaga tcggagatgg ccggagtgag agcgggggcg
     6121 ctcacctgga gcgcccccgc ccgacccaga ctagcggacg cctgcgcgcc tcagcgacgc
     6181 cgggctgaag tcgctcggcg tgtacttcac actgaagtca taggtgcccc gttcctcgtt
     6241 gttgaggcca atgccgaggt agcgaccgga ctgcaggtcc atggtcagct cgagcgtcgt
     6301 ccagaacgag ggaacgtcat agtagttgat ggaatgggct tcctggacgc gccagatctg
     6361 gtcgcggttg tcgtagcagt ccaccaccat ggcctgccag gagtcctcgt cgatatagaa
     6421 ggtacggcgc ttgtagacgt ggcgcgtgcc ctccttcagc gtggcgtcca ccacccacac
     6481 ccggtgcagc tcgtagcgcg cgtgctcctg gttgatgtgc agcggcttga ggatgtcgct
     6541 gtacttcagc ttgttgctgt gcagcttgta gctgttgtac gggaccagga tctccttgcg
     6601 gccgaccagc gtccagttgt agcgctcggg gctgccgttg aagatgtcga gctggtccga
     6661 ggtcgccagg ccgtccgagg cggtgcgcgg gttgtcgtgc gcgacgttcg gcgcgcgccg
     6721 tacgcggcgc tggcccgggt tgtacagcca ggccttgcgg ttctcgcgcg cctggttgag
     6781 agtctcgtgc accagcagca cttcgccggc gagacgcgac ggcgcggtga cggtctgctt
     6841 gaagaagatg atgacgttgt cgaggtcctc ctcgcgagca ccggccaggc cgtacgcccc
     6901 gaggtactcg tcagtgaaca tcaccatcgt gtagttgccg tcgcgggtca ccggtgcctg
     6961 gccgatggtg cgtcgcgcgc tgtcggtgcg ataacgggcg atgtggttcc agatggcgtg
     7021 caggccgttc tccggaatcg ggaaaggaat gccctccgca ccgccgctga agccgttacc
     7081 gctctcgatg agatgggtgc tgccggcgat gcgcttggtg gcttcgtaaa tgcgctccgg
     7141 cacggccgcg ctgcgccgcg tcggatagac gtccatgaag tattccggat aggtccggaa
     7201 catcgccttg tggccctcgg tgaggatgtc gtcgtactgc gccatgttcg cggcggtgat
     7261 gcggtacagc ggcttgtcgt ccgcgtacgg atccgggtga tggccacccg atacgaaatt
     7321 cgggaagccg gcctcggcgg gagtgcggag cccgcccgtc cacgccggga tggtacccgc
     7381 ctcgttgccc gcccggatcg cgcccatggg cgtcaggtcg gcgtccagcc ttgcaatctg
     7441 ctcggccgac aaccgctcgg cggacgccgg tgcggcgagg cccaccaggg ccagcgcggc
     7501 gagagcctta aggagtctga gattggtcat gggatgactc tcctggtaat cctagaacga
     7561 gaccttggcc gtgaaggaaa tgaagtcacg gtcatggccc gtgttgtaga tgcccgcacc
     7621 gccgaactgc gcgtagttga gctcgagctc gtacttgttg cgcagggtac ccttgagcgc
     7681 caccgataac gccgtgcggt cctcgatgaa gttgccgccc ggtccggggc tgttgccatt
     7741 gacgtcatgc gaccagccga cgatcggcga gaggctccac gggccgatgg cgttgttgta
     7801 gaccagcgcg ccgcggaagc ggtagcccca cgaggtcttg tgcgggaacg cgctgcgcgg
     7861 atccagctcg aagaaatgtg cggcggacaa gggcacgttg ccggacagcg aggtgccttc
     7921 cccgttcagc cgcaggccgc ggttgttcgg accgccgctg ttggtgtcgg gcaggtcggg
     7981 cacgtagttc atgccgagct cgagcacgaa ggcgccggtg ccggcgccga gcagcgggcc
     8041 ggacaggtag gtgaacgtcg tctgggcctg ccagatgtcc agctcgacgt aacccgggat
     8101 gacggtgttc aggccgaaca cgccgatctg gttgaagcag ccgaggttgc cgcgggcatt
     8161 cgggttggtc gggcagacat aggaacccgc cgtcggcgac aggttgtcca gcgggctgag
     8221 cgccgcgaac agcagctcga cgtcgtccac ctgcatcggc gagtccggct tgtagctgac
     8281 ttcgccctgc caggcgatgc cggtattgaa caggtagccg ctgaagctga cgccgtacag
     8341 gtccaggtcc tccgggtact cgaggaagta ccgcgaggtc tgcgccaact cgtcctgcgc
     8401 aaaggcgttc gccaaccctg ccacacccgt cgaaccgagg ctcaggaagg tgttggccgc
     8461 cacggtcgcc cgctcgcgca cgcgcgcctg ggcgacgtcg ccgccgagat tggccgcggc
     8521 ggccatgccc tgcgccgtcg cggtgttgac cgccgcgtcg aaggacagcc ccgaggccag
     8581 cccgatggct gccgcctggg cggcggtcgc caccgcggct gcgttgccga agccggcctg
     8641 cgtgcccgtc aggccgctga ccacgggcag gcggctgtga tagcgcaggt aatagaagcc
     8701 gaactccatg ccgcccatga tgctgtcggc gaaatagcga agtgcgagac cgtactggcc
     8761 gccgtcgtcc ggtgcggcgc tggcgccgcg cggcacgaac acgaagtcgg agtcgaagct
     8821 gccgcccagc ggggtccagt cggtgccgag gtccgaccag gcgccgaagc cgagcatcgc
     8881 cttggtggcg cccttgccgg cgaggtcgtt ggtcgagaag tagctgccga ccgggtccgg
     8941 caccacgttc tgccagtcgt actggtagta ggcctcgatg ctgaacttgt cggtcggcga
     9001 gacattgaag tacgccatgc cctgcggcac cagcgcttcc ttcagctccg agcccggcac
     9061 ccgcagcttg ccgacgtcca ccgggttggt gaggttggcg ccgccctgga tgaaggtgct
     9121 ctcgccccag ctgatcacct ggttgccgat gcggaactcg gccggcatgc tgcccaggtt
     9181 gaacggcacg tagacgaagt agtcgagcat cttgccgccc tgctcgacgc gggtcagccc
     9241 gccctcggaa atttcggtgc ggttggtgtt gccggcctca ccgtcgtaga aggccgtgcc
     9301 acgggcgaac agtccccagg aatcgcggaa cctcagctcg atttccgacg tggccttgat
     9361 ggcactcgag accaggccgg tatcgaagtt gagattgccg tcgtcgttgt tcgcactgcg
     9421 tccgcagccg ccgttggcat tcgcgatgag ccggcaatcc gggctctgta cgcgccagat
     9481 ggcgccgtag gacagcgtgg tgtcccagct gccggtccag ttgtcagtct gaattggcac
     9541 tgccgacgcc ggcgctgcaa agcctgcagc aatcacggtc gcgatggcag tcgccagaag
     9601 gcgtccgttg cccggacggg ccctggaggg ttgcgcgctc atgaggactc cccagtcttt
     9661 ctgtttctga attgtg
//
LOCUS       DAOMPG010000078         9660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3042_length_9660_cov_5.013847, whole genome shotgun sequence.
ACCESSION   DAOMPG010000078 DAOMPG010000000
VERSION     DAOMPG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_9660_cov_5.013847"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLS34_05865"
     CDS             <1..349
                     /locus_tag="PLS34_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRP87030.1"
                     /translation="IPERLAGLDGFAGCATVVIEALMTPNGVFVLALVGGSLAAGVAA
                     GSFQPRAYSWRRYLAALLGGALMGWGAMVALGCTVGVLLSGISAFALSGWVFGAAVTA
                     GVFISLPVRRLLG"
     gene            complement(390..1214)
                     /locus_tag="PLS34_05870"
     CDS             complement(390..1214)
                     /locus_tag="PLS34_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRP87031.1"
                     /translation="MRNRAAGLLLLASLLLATSAAAGSDRQYVDAMAREHAGDLPVAG
                     AAGGPAAGQVHGEAIEADGVRGYLARPAGTPRAGLIVIHEWWGLNDNIREMSKRLAAE
                     GYLALAVDLYGGEVAADAAQARDLMSAAMGDAEQLDAALRAAFRWLQDEGGVERVGSI
                     GWCFGGALSLRTAIMLPEELDAAVIYYGRLETDPARLAPLRMPILGIFGGQDRGIPLQ
                     SVHEFETVLQTLGKTAQIVVYDDADHAFANPSGTRYEPEAAADAWRRTLEFFAEHL"
     gene            complement(1211..2470)
                     /gene="glgC"
                     /locus_tag="PLS34_05875"
     CDS             complement(1211..2470)
                     /gene="glgC"
                     /locus_tag="PLS34_05875"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRP87032.1"
                     /translation="MKTGKPGRYVSSLTRDTVALILAGGRGSRLKALTLSRAKPATPF
                     GGKYRIIDFPLSNCVNSGIRRIAVLTQYKAHDLIVHLQRAWGYFRGEFGEFVEILPAQ
                     QQIDSSWYKGTADAVFQNLDYLSRREPEYVLILAGDHIYKMDYGLMLAQHAESGADIT
                     VGTVEVPVSRAQEFGIVTIDATNRITRFVEKPLHPEPIPGREGIALASMGIYVFSFEY
                     LERILRADAMDAGSAHDFGRNIVPGCIEAGDHVHAYVFQDAATKVQLYWRDVGNVDAF
                     YEANIELTYVAPELNLYDQEWPIWTYQAQLPPAKFVLDDDVRHGRALNSMVSGGCIIS
                     GALVRESVLFSNVRVAERSLVERSIILPNVDIGPDCRIVHTIIDEDTDVPAGTVIGED
                     AAEDRRRFYVTKEGVVLVTREMMEALL"
     gene            2598..4106
                     /gene="glgA"
                     /locus_tag="PLS34_05880"
     CDS             2598..4106
                     /gene="glgA"
                     /locus_tag="PLS34_05880"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRP87033.1"
                     /translation="MPPKLKVLFVVAETWPLVKTGGLADVVPALARALIAQGHDARLL
                     MPGYQAVEERFAGKAAGRFFDALPGLSRVRLIRGELPDYGIPMWLLHCPALYDRPGGP
                     YADERGDDWPDNSLRFGMLGKVATLFASGQGLDGWKADVLHGHDWHAGLASAYAAWDR
                     RVTAGTVFTIHNLAYQGNFDPAVGRALGVQPRALQMDGLEFYGHLSFMKAGLWYSDRL
                     TTVSPTYARQVQTEEYGCGMDGVLRKRQAALTGILNGVDEQVWSPATDPLLPARYSAA
                     QLAEGKARCKLELQDRFGLPRGPDIPIIGTVGRMTSQKGWDLLESAAPALLADKIQLV
                     MVGSGDHALEERLRALAARHPGWLGVHIGYDEALSHLVIAGADVFTVPSRFEPSGLTQ
                     MYSQRYGTPPVVRHTGGLADSVTAATPASIADGSGTGFFFTRPDAAALEQALREAVAL
                     YRGNPSAWRALQANGMARDFSWGAPARAYAEVYEAALAAGRTRRKAASAPTD"
     gene            4498..5043
                     /locus_tag="PLS34_05885"
     CDS             4498..5043
                     /locus_tag="PLS34_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87034.1"
                     /translation="MIRSAPVLASALALFAPANPAALAAEPGGSCLPGEGGHLRMEVS
                     GDFDATLDWPNEGTHCDGGPRPAGDALRLMFSREDDGLLVVIGITGLQRGQTGAGLLA
                     NVTLVRQGLGRFYGTLGADACVVAVAENTPVDGLADAWRVRGQGYCAAAIEAIAREGE
                     IRIAPFEFSGLAFWPDEQEED"
     gene            5045..5515
                     /locus_tag="PLS34_05890"
     CDS             5045..5515
                     /locus_tag="PLS34_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRP87035.1"
                     /translation="MRRYLFPALCVIALVLALHTMTLASGVSDEFPVERLEIVTSDGD
                     RFAFSAHIAATPEARRRGLMYVTRLGPDEGMLFDFESPRPVGMWMKNTPLSLDMLFIA
                     SDGRIARIAARTRPFSTELIASGVEVRAVLEINGGRAAELGIAAGDRVLHPIFD"
     gene            complement(5598..6995)
                     /gene="hemN"
                     /locus_tag="PLS34_05895"
     CDS             complement(5598..6995)
                     /gene="hemN"
                     /locus_tag="PLS34_05895"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRP87036.1"
                     /translation="MTIQTAEFDLDLIKRYDARGPRYTSYPTAVQFHDGFGEEDYKRF
                     ALASNGDDPESQPLSIYVHVPFCHSLCYYCGCHKIITRHQHSADPYVEHLVEEMRLQG
                     RLFDPRRRVDQLHFGGGTPTFLDEGQFRKVMDGLFRNFGLHHGEQREYSIEIDPRSVD
                     KESIPRLAEMGFNRLSLGIQDFDPEVQKAVNRLQDEEWTLFLIRQAREHGFRSVSVDL
                     IYGLPKQTAASFTHTLESVVQAKPDRIAAYSYAHLPEMFRAQRLIRPEDMPSAAEKLA
                     LLTRTVEFLTGRGYEYIGMDHFALPEDDLVKSLQDGTLHRNFQGYSTHAELDLVGLGV
                     SAIGKVGNCYAQSIKLRHDYYDAIDAGHVPILRGVELTADDVLRREVIHALMCGGVAD
                     MRKIEARHGIRFEEYFKRELEAMIPLANDGLVELDAGAIRVTPRGRFLLRSIAMPFDA
                     YLAARLAEQRFSKVI"
     gene            complement(7112..7483)
                     /locus_tag="PLS34_05900"
     CDS             complement(7112..7483)
                     /locus_tag="PLS34_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87037.1"
                     /translation="MPSRPIAVCLLLALPLAGVAGCAAIEDQTDYHRHSMSDLRDDRR
                     NPGRLLFEATTSSRYPADSASAETVRMEWLAAWMKRSGSCPQGWEILSRSPIDPAEVH
                     ARRHDLRYEIQCTEAAAPAGH"
     gene            7708..8820
                     /locus_tag="PLS34_05905"
     CDS             7708..8820
                     /locus_tag="PLS34_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP87038.1"
                     /translation="MRRTLIAGIAAGGLLLAGPAFAAEKPGQWYVAPMASVIWTDNDR
                     LVDDDIGAALSMGRAISDNINLELHAFGYQLEGLDDTDLWGIGFDVMRVFYRPERISP
                     YLSGGFGWNVKNSEFASDSKNMFVGAAFGILADLTPSVALRSEVRYRVDYANGWGGET
                     FNDLIFNLGLQIPFGQPYAEPVVAAPPPPPPPPPPPADSDGDGVPDSRDRCPGTPAGV
                     AVDADGCPLDTDGDGVPDFRDACPGTPAGARVDSRGCPITDVISLEGVTFAFNSDQIT
                     ADERGRLNEAVDILRRYPDLKVEVAGHTDSVGNPAYNQGLSERRARSVLEFLAAAGID
                     RSRLTARGYGQTSPVADNATDAGRAMNRRVELRVVD"
BASE COUNT         1487 a         3277 c         3291 g         1605 t
ORIGIN      
        1 gatacccgaa cggctggcgg gcctggacgg cttcgccggc tgcgccaccg tggtgatcga
       61 ggcgctgatg acccccaacg gggtgttcgt gctggcgctg gtgggcggca gcctcgccgc
      121 gggcgtggcc gccggcagct tccagccacg cgcctactca tggcgccgct acctggcggc
      181 gctgctcggc ggcgcgctga tgggttgggg tgcgatggtc gcgctcggct gcaccgtcgg
      241 cgtgctgctc tcggggatct ccgcctttgc cctctccggc tgggtgtttg gcgcggcggt
      301 gacggcgggg gtattcatca gcctccccgt gcgacgcttg ctcggataaa ccggggaaaa
      361 cggggtcaga ccccgttttc cccggctccc tacaggtgct cggcgaagaa ctcgagcgtc
      421 cgccgccacg cgtcggcggc cgcctcgggc tcgtagcgcg tgccggacgg gttcgcgaaa
      481 gcgtggtcgg cgtcgtcgta caccacgatc tgcgcggtct tgccgagggt ctgcagcacg
      541 gtttcgaact cgtgcacgga ctgcagcggg atgccgcgat cctggccgcc gaagatgccg
      601 agaatcggca tcctgagggg cgccaggcgg gccgggtcgg tctcgagccg gccgtagtag
      661 atgacggccg cgtccagttc ttccggcagc attatggccg tacgcagcga cagggcgccg
      721 ccgaagcacc agccgatgct gccgacgcgc tcgactccgc cttcgtcctg cagccagcgg
      781 aacgccgccc gcagcgcggc atccagctgc tcggcatcgc ccatcgcggc ggacatcagg
      841 tcacgagcct gggccgcatc cgccgccacc tcgccgccgt acaggtccac cgccagggcg
      901 aggtacccct ccgccgcaag gcgtttactc atctcgcgga tattgtcgtt caggccccac
      961 cactcgtgga tgacgatcag gccggcccgt ggcgttcccg ccggccgcgc caggtagccg
     1021 cgcacgccgt ccgcctcgat ggcctcgccg tgcacttgcc cggcggccgg cccgccggcc
     1081 gcacccgcca caggcaggtc accggcatgc tcgcgcgcca tcgcgtcgac atattgccgg
     1141 tccgatcccg ccgccgcgct tgtcgccagc aggagcgatg caagcaacag cagccctgct
     1201 gcccgattcc tcataacaaa gcctccatca tttcccgcgt caccagcacg acgccttcct
     1261 tggtcacata gaagcgccgc cgatcctccg ccgcgtcttc gccgatcacc gtcccggccg
     1321 gcacgtcggt gtcttcgtcg atgatggtgt gcacgattcg acaatccggg ccgatatcca
     1381 cgttgggcag gatgatcgag cgttctacca gcgagcgctc cgcgacgcgc acattgctga
     1441 acagcaccga ctcacgcacc agcgcaccgg agatgatgca accgcccgag accatcgagt
     1501 tcagtgcccg cccatggcgc acatcgtcgt cgagcacgaa cttggcgggc ggcaactgcg
     1561 cctggtaggt ccagatcggc cattcctggt cgtagagatt gagctccggc gcgacgtagg
     1621 tgagctcgat gttggcctcg tagaacgcat ccacgttgcc gacgtcgcgc cagtagagct
     1681 gcaccttggt cgcggcgtcc tggaacacgt aggcatgcac gtgatcgccg gcctcgatgc
     1741 agcccggcac gatattccgg ccgaagtcgt gcgccgaacc ggcatccatc gcgtcggcac
     1801 ggaggatgcg ctccaggtac tcgaagctga acacgtaaat gcccattgac gcgagcgcga
     1861 tgccttcgcg cccggggatc ggttcgggat gcagcggctt ctcgacgaaa cgcgtgatgc
     1921 ggttggtcgc gtcgatggtg acgatgccga actcctgggc acgcgagacc ggtacttcga
     1981 ccgtgcccac cgtgatgtcc gccccgcttt cggcgtgctg cgcgagcatc aggccgtagt
     2041 ccatcttgta aatgtggtcg ccggccagga tcagcacata ctcgggctcg cggcgagaca
     2101 ggtagtccag gttctggaac acggcgtcgg cggtgccctt gtaccaggac gaatcgatct
     2161 gctgctgggc cggcaggatc tcgacgaact cgccgaactc gccgcggaag taaccccacg
     2221 cccgctgcag gtggacgatc aggtcgtgcg ccttgtactg ggtcagcacc gcgatgcggc
     2281 gaattccgga gttcacgcag ttggacaagg ggaaatcgat gatccggtac ttgccgccga
     2341 acggcgtggc cggcttggcg cgcgacagcg tcagcgcctt caggcgcgac ccgcggccgc
     2401 cggcgagaat cagcgcgacg gtgtcgcggg tcaggctgct gacgtagcgg ccgggcttac
     2461 cggtcttcat gacacgcccc tgtgctgttg gcccctcagc ataccttaac aggcgcccgc
     2521 cgccccgcca tgcggaggcg ccgcagtgcg gcgcccggct gtgctaaatt gccgcgaatt
     2581 tcgccatccc ggtcgacatg ccccccaagc tcaaggtctt gttcgtggtc gccgagacct
     2641 ggccgctggt caagaccggc ggactcgcag acgtcgtgcc ggcgctggcg cgcgcgctga
     2701 tcgcgcaagg ccacgatgcg cggctgctga tgccgggcta ccaggcggtc gaggagcgat
     2761 tcgcaggcaa ggcggcagga cgcttcttcg acgccctgcc cggactcagc cgcgtccggc
     2821 tgatccgcgg cgaactgccc gactacggca ttccgatgtg gctgctgcac tgtcccgcgc
     2881 tgtacgaccg acccggcggg ccctacgcgg acgagcgcgg cgacgactgg cccgacaaca
     2941 gcctgcgctt cggcatgctc ggcaaggtgg cgacgctgtt tgcttcgggc caggggctcg
     3001 acggctggaa ggccgatgtc ctgcacggcc acgactggca tgccggcctg gcttccgcct
     3061 acgctgcctg ggaccgtcgg gtgacggccg gaaccgtgtt caccatccat aacctcgcct
     3121 accagggcaa cttcgacccc gccgtcggcc gtgcactggg cgtccagcca cgcgcgctgc
     3181 agatggacgg gctggagttc tacggccacc tgagcttcat gaaggccggc ctgtggtact
     3241 cggaccgtct caccaccgtg agccccacct atgcgcgcca ggtgcagacg gaagagtacg
     3301 gctgcggaat ggacggcgtg ttacggaaac ggcaggccgc gctgaccggc atcctcaacg
     3361 gcgtcgacga gcaggtctgg agcccggcta ccgacccgct gctgccggcc cgatactcgg
     3421 ccgcgcagct cgcggagggc aaagcgcgtt gcaagctcga gttgcaggac cgcttcggcc
     3481 tgccgcgcgg ccctgacata ccgatcatcg gcacggtcgg ccgcatgacc tcgcaaaagg
     3541 gctgggacct gctggagagc gcagcgccgg cgctgctcgc cgacaagatc cagctggtga
     3601 tggtcggcag cggcgatcat gcgctcgagg agcggctccg tgcgctggcg gcccgccacc
     3661 ccggctggct cggggtgcac atcggctacg acgaggccct gtcgcacctg gtgatcgcgg
     3721 gcgccgacgt ctttacggtg ccgtcgcgtt tcgaaccctc cgggctgacg cagatgtaca
     3781 gccagcgcta cggcaccccg cccgtggtca ggcacaccgg ggggctggcc gacagcgtca
     3841 cggcagcgac gccggccagc atcgcggacg gctcggggac gggattcttc ttcacgcgcc
     3901 ccgacgccgc ggcgctcgag caggccttgc gcgaggcggt ggcgctgtat cgtgggaatc
     3961 cctctgcatg gcgcgcgctg caggccaacg gcatggcacg cgacttcagt tggggcgcac
     4021 ccgcccgggc ctatgcggaa gtttacgagg ctgcgctggc cgccggccgg actcgccgca
     4081 aggcggcgtc cgcgccgacc gactgagccg gtaaaggcgg cagatcggcc tgggcgtgcc
     4141 gggacacgga gataaggcaa ccttggggcc ctgagacccg cgagaacagt tggcgcttat
     4201 cgcctcgagt gcgaaccctt gacccgggac gcccgcggcc gacctgcctg gaaccggtgg
     4261 cccccgatga aggggaggcg gcgcggaaac gaaggatagc ccgaatcccg ccgcgatgcc
     4321 tagcgaaacc cgcggtggat gtccgtgttg cttccaaatc aataatttat gtaaacaatt
     4381 tcgaccggga gggcgaagtt ttcgcggctt ttggcctcgc ccgccctcct ttttggctat
     4441 tgactccgcc cggcgaaggt ctatgctcgc gcgctccatg ttgctttgca tcaagcgatg
     4501 atccgatccg cccccgttct ggcctccgcc ctggcgttgt tcgcgccggc gaacccggcc
     4561 gcgctcgccg cggaacccgg cgggagctgc ctccccgggg aaggcggaca cctgcgcatg
     4621 gaagtcagcg gcgacttcga cgcaacgctc gactggccca acgaaggcac gcactgcgac
     4681 ggcggtccgc gcccggccgg cgatgcgctg cggctgatgt tcagccgcga ggatgacggc
     4741 ctgctggtgg tgatcggcat caccgggctg cagcggggcc agaccggcgc gggactgctc
     4801 gccaacgtca cgctggttcg ccagggactg ggacgcttct acggcacgct gggcgccgac
     4861 gcctgcgtgg tcgccgtggc cgagaacaca cctgtcgatg gactggcgga cgcctggcgg
     4921 gtgcgtggcc agggatactg cgcggcggcg atcgaggcca tcgcccgcga gggcgagatc
     4981 cgcatcgcgc cgttcgaatt cagcggcctg gcgttctggc ccgacgagca ggaagaggac
     5041 tgagatgcgt cgttacctgt tccctgcgct atgcgtcatc gcgctggtcc tcgcgctgca
     5101 caccatgaca ctcgcgtccg gcgtctcgga cgagttcccc gtcgagcggc tcgaaatcgt
     5161 cacgtccgac ggcgatcgtt tcgcgttcag cgcgcacatc gcggccacgc ccgaggcgcg
     5221 ccggcgcggc ctgatgtacg taacgcggct gggtcccgac gagggcatgc tgttcgattt
     5281 cgagtcgccg cgcccggtcg gcatgtggat gaagaacacg ccgctgtcgc tggacatgct
     5341 gttcatcgcc agcgacgggc ggattgcgcg gatcgcggcg cgcacccgcc ccttctccac
     5401 cgaactgatc gcctcgggcg tcgaggtgcg cgcggtgctc gagatcaacg gcggccgcgc
     5461 cgccgagctc ggcatcgccg ccggtgaccg ggtgctgcac ccgatcttcg actgaaaacc
     5521 ggggtcagac acctatttcc gaagcgaaat aggtgtctga ccccggtttt tgaccccggt
     5581 tttcgtcccg attgtcccta gatcaccttc gagaagcgct gctcggcgag ccgtgccgcc
     5641 aggtaggcgt cgaagggcat cgcgatggag cgcagcagga agcggccgcg cggcgtcacg
     5701 cggatggcgc cggcgtcgag ctcgaccagc ccgtcattcg ccagcgggat catcgcctcg
     5761 agttcgcgct tgaagtactc ctcgaagcgg atgccgtggc gcgcctcgat cttgcgcatg
     5821 tcggccacgc cgccgcacat cagcgcatgg atcacctcgc gccgcagcac gtcgtcggcg
     5881 gtcagctcga cgccgcgcag gatcggcacg tgtccggcat cgatcgcgtc gtagtaatcg
     5941 tggcgcagct tgatgctctg cgcgtagcag ttgccgacct tgccgatcgc gctcacgccg
     6001 aggcccacga gatccagctc ggcgtgcgtt gagtagccct ggaaattgcg gtgcagtgtg
     6061 ccgtcctgca gcgatttcac caggtcgtcc tccggcagcg cgaagtggtc catgccgatg
     6121 tattcgtagc cgcggccggt gaggaactcg acggtgcgcg tcaacagcgc cagcttctcg
     6181 gccgccgagg gcatgtcctc ggggcggatc agccgctgcg cgcgaaacat ctcgggcagg
     6241 tgcgcgtaac tgtaggctgc gatgcggtcc ggcttggcct gtacgacgga ctccagcgtg
     6301 tgggtgaagc tcgcggcggt ctgcttcggc agcccgtaga taaggtccac ggagacggag
     6361 cggaagccgt gctcgcgcgc ctggcggatc aggaacagcg tccactcctc gtcctgcagc
     6421 cggttcaccg ccttctgcac ctcgggatcg aaatcctgga tgcccagcga cagtcggttg
     6481 aagcccattt ccgccagccg cgggatggat tccttgtcca cgctgcgcgg gtcgatctcg
     6541 atggagtatt cgcgctgctc gccgtgatgc aggccgaagt tgcggaacag cccgtccatc
     6601 accttgcgga actggccctc atcgaggaag gtcggggtgc cgccgccgaa atgcagctgg
     6661 tcgacgcgcc gccgcggatc gaacagcctg ccctgcaggc gcatttcctc caccaggtgc
     6721 tccacgtagg gatcggcgct gtgctggtgg cgggtgatga tcttgtggca gccgcagtaa
     6781 tagcagaggg aatggcagaa ggggacgtgc acgtagatcg agagcggctg cgactcgggg
     6841 tcgtccccgt tgctcgccag cgcaaagcgt ttgtagtcct cctcgccgaa gccgtcgtga
     6901 aactgcacgg cagtcggata cgaagtgtag cgcggcccac gagcgtcata gcgctttatc
     6961 aggtcgagat cgaattcagc cgtctggata gtcatgcatc accgcatcgt cgttggggca
     7021 gccgagggat cccgcgaatc ccgagcgggc ctggcattcc gtaaccgtac atcatctacc
     7081 gtaccgtcgg cccgggaaat cgtggattgt ctcaatgccc ggccggcgcc gccgcctcgg
     7141 tgcattgtat ttcgtagcgc aggtcgtggc gccgggcatg gacctcggcc gggtcgatcg
     7201 gcgaacgcga caggatttcc cagccctgtg gacagctgcc ggagcgcttc atccaggccg
     7261 ccagccactc catgcggacg gtctcggccg acgcgctgtc ggccggatac cggctcgaag
     7321 tcgtcgcctc gaacaacagc cggcccggat tgcgccggtc gtcgcgcagg tcgctcatgg
     7381 agtgccgatg gtaatccgtc tggtcctcga tggcggcaca acccgccacc ccggcaagcg
     7441 gcaaggccag cagcaggcat acagcgatcg ggcgcgatgg catccttggt tcacctcctg
     7501 ccagacgagg tccgaattct actcccaagc cggggccgag gggccgggct tgcgccgcag
     7561 cattcgagcc cggcagcaat cccttggaac cccggcggcg ccggaattcc cgttttcagc
     7621 tataattccc gcatacgccg tggcctggag gctgcggaaa gcggcatcgg gcgatagacg
     7681 ccccggacct gaaagggagc gagaacaatg agacgaactc tgatcgcagg gatcgcggca
     7741 ggcggtctgc tgcttgcggg cccggccttc gcggccgaga agcccggcca gtggtacgtg
     7801 gcgccgatgg cgtcggtgat ctggacggac aacgatcggc tggtggatga cgacatcggg
     7861 gcggcgctgt cgatggggcg cgccatctcc gacaacatca acctcgagct ccacgccttc
     7921 ggctaccagc tggagggcct cgacgatacc gatctgtggg gtatcggttt cgacgtgatg
     7981 cgggtgttct atcgccccga gcggatttct ccctaccttt cgggcggttt cggctggaac
     8041 gtcaagaact cggagttcgc ttcggacagc aagaacatgt tcgtcggtgc ggccttcggc
     8101 atcctggccg acctcacgcc gagcgtcgca ctgcgctcgg aggtccggta ccgcgtcgat
     8161 tacgccaacg gctggggcgg cgagactttc aatgacctga tcttcaacct cggcctgcag
     8221 attccgttcg gccagccata cgccgagccg gtcgttgcgg cgcccccgcc gcctccgccg
     8281 ccgcccccgc cgccggcgga ttcggacggt gatggcgtcc ccgactcgcg cgaccgctgc
     8341 cccggcacgc cggccggcgt cgcagtggac gcggacggat gcccgctgga tacggacggt
     8401 gacggcgtac cggacttccg cgacgcctgc cccggcactc ccgcgggcgc gcgcgtcgac
     8461 agccgcggct gcccgatcac cgacgtcatc agcctcgagg gcgtgacctt cgccttcaac
     8521 tcggaccaga tcacggcgga cgagcgcggc aggctgaacg aggccgtcga catcctgcgc
     8581 cgctatccgg acctgaaggt cgaagtggcg gggcacacgg actcggtcgg caacccggcc
     8641 tacaaccagg gcctgtccga gcggcgtgcc cgctccgtgc tcgagttcct ggccgccgcg
     8701 ggcatcgatc gcagccgctt gacggctcgc ggttacggcc agacgtcccc ggtcgcggac
     8761 aacgcgacgg acgccggccg ggcgatgaac cgccgcgtcg agctgcgcgt ggtcgactga
     8821 agcatccgga ctcgcgactt cggttgcggg cgcaacttca gccccggtct cgcaagaggc
     8881 cggggctttt tcatgccccg tcgccgggct cctggttccg cttgaccgcg ggggtgggcc
     8941 gccaggcgta tcgttcgggt tgaccgcggg ggcgcgccgc catgggcgcg cttcgttcgg
     9001 gttgaccgcg ggggcgcgcc gccatgggca cgcttcgctc ggcgaattcc aagctcactc
     9061 agcgcgccca tgacgccccg cctccgctgc cgagtcgatg cgcacggcgt cgaccgctgc
     9121 ctggctcggc gggactgcct cgcggcgggc ggaatgttgc ctttatgtgg cgaactcgtc
     9181 tggtggctga tcgacaccag ggtcgccagg acgtcaaggc gccgcggccg tcagctgcgg
     9241 cgacgaatcg ccagcctgac gccagtcggc gtggtttgcg gccgtggcga tgctcagcgg
     9301 cggcgcggag gtcgggcgtc gccatcgtct cagtcgtagc gggtgcgggg tgtcataggc
     9361 cggccgagcg agtttttcct tgtccgagcg caggccggcc tatggcgccg cgcgcccgcg
     9421 gtcgaccgga aaaccggtgt ctgaccccgg tttgacggca caaaaaaaac cggccccact
     9481 ggggggccgg tcaggtatgc ggtcagtgtt attagagctt ggcgttgtcg gcctagaagc
     9541 gctgagtgta gcgggcgtag gcgacacgac cccagccgtt gtacaggctg tagttgtagt
     9601 cgcggccgcc gaaggcgacg aactcgggca gcttctcggt gaggttctgc acaccgatgg
//
LOCUS       DAOMPG010000079         9634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3052_length_9634_cov_3.841320, whole genome shotgun sequence.
ACCESSION   DAOMPG010000079 DAOMPG010000000
VERSION     DAOMPG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_9634_cov_3.841320"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLS34_05910"
     CDS             <1..302
                     /locus_tag="PLS34_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRP87039.1"
                     /translation="TGTMAATRRLLARIPGLATSELPAGCCGAAGENFLSRPGMADAL
                     LAPLLDLLSEEPPDALVTSNIGCAMHFAAGLRRRGLEVPVLHPASLVADALQSAT"
     gene            299..943
                     /gene="coq7"
                     /locus_tag="PLS34_05915"
     CDS             299..943
                     /gene="coq7"
                     /locus_tag="PLS34_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HRP87040.1"
                     /translation="MSVRRLTPLDRVLEQVDQAVRTVLGPPPPASRPNPAEGVPDAPL
                     TDRERRHAAGLMRVNHAGEVMAQGLYQGQALTARLAEVRGAMEQAAVEEFDHLAWCEQ
                     RLAELRDRPSVLGPVWYTGAFLLGAGAGLAGDRWSLGFVSETEAQVINHLEDHLGRLP
                     RADQRSRAIIGQMKLDEARHGAEARAAGGLPLPAPARGLMAVMSKVMTRTAYWV"
     gene            1056..1718
                     /gene="crp"
                     /locus_tag="PLS34_05920"
     CDS             1056..1718
                     /gene="crp"
                     /locus_tag="PLS34_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HRP87041.1"
                     /translation="MGEPLGRDNDIPAMSRFLELCRVRSVPSKTVVIHAGDLPDVLYY
                     IVDGSVEVMIEDEDGNEMVLAYLNKGQFFGEMGLFYEQPTRSAWVRTRTACELAEMTY
                     PRFRQVAAENPALLFELATQLATRLERTNRKLGDLAFVDVTGRIAHAIMDLCSEPDAM
                     THPDGMQIKVSRQELSRLVGCSREMAGRVLKVLEEQGLLRARGKTIVVHGVRPEHKPR
                     RG"
     gene            complement(1719..2402)
                     /gene="rpe"
                     /locus_tag="PLS34_05925"
     CDS             complement(1719..2402)
                     /gene="rpe"
                     /locus_tag="PLS34_05925"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRP87042.1"
                     /translation="MLDYRIAPSILSADFARLGEEVDAVLAAGADMVHFDVMDNHYVP
                     NLTIGPMVCEALRNWGIEAPIDVHLMVKPVDGLIESFARAGASWITFHPEASEHVDRS
                     LQLIRDLGCRAGLVFNPATPLSWLDYLADKIDMVLIMSVNPGFGGQKFIPTALRKIAE
                     ARQFIDDSGLPIRLEVDGGVKVDNIADIARAGADTFVAGSAIFGASDYASTITAMRRE
                     LAGADLGGD"
     gene            complement(2412..3185)
                     /gene="djlA"
                     /locus_tag="PLS34_05930"
     CDS             complement(2412..3185)
                     /gene="djlA"
                     /locus_tag="PLS34_05930"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HRP87043.1"
                     /translation="MNWTGKLVGVIIGSLAGPVGAAVGLFLGHQFDRGVGGARRESFD
                     GVGQVFFETTFAVMGHVAKADGRVSEAEIQAARGIMSRMRLDATQVQEAIRLFNIGKA
                     PDWPMERELARLADALGSRRTLRRAFLEIQVEAALSNPYMGAETRDMLWRIASALDLG
                     RVELAQVEALLRFGRAAGGDRQVQPRSTAADAYRVLGIEPAASDAEVKLAYRRLMNQH
                     HPDKLRARGMPESMIPVAEAKTREIRAAWETLRAARGMR"
     gene            3358..4260
                     /locus_tag="PLS34_05935"
     CDS             3358..4260
                     /locus_tag="PLS34_05935"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRP87044.1"
                     /translation="MSAMFQSDLPGLELLHRGKVRDVYAVDDRTLLIVATDRLSAFDV
                     VLPDPIPHKGEILTQTALFWFHRTRALVPNHLLERPLDSVLPGGVDRDDYRLRSSVTR
                     RLRPLPIEAVARGYLIGSGWKDYQAGGSVCGVALPPGLRLADRLPEPIFTPATKAAAG
                     EHDENIAYEQVVELVGPGLAAQVRDLTLRIYGEAAAYARERGIIIADTKFEFGLDEHG
                     ELYLIDEILTPDSSRFWPADEWRPGASPPSFDKQYVRDYLETLAWDKQAPGPRLPAEI
                     IARTADKYREAYRRLTGDDELFGA"
     gene            4257..5549
                     /locus_tag="PLS34_05940"
     CDS             4257..5549
                     /locus_tag="PLS34_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRP87045.1"
                     /translation="MSDTLQSRVERLLWNKESAAEPPLRRALRLPARFVYALTRDLAE
                     GQLTLRAMSLVYTTLLSLVPLLAFSFSVLKGFGVHRQVEPLLYEFLLPLGEHGAQITD
                     QIIAFVENVRGGVLGGIGLALLMFTVISMIQKVEDTFNYIWQVQKSRNLARRFSDYLS
                     VILVGPVLMVTAMGLLATINSSDIMQAIAEVQPFGTLLAVIGRLAPFTLVVVVFAFVY
                     AFVPNTRVRLGAALVGAVLAGAAWTLGGSLFATVIVGSTRYAAIYSSFAIAIFALIWL
                     YLSWLVLLLGAQVAFYVQNPRRLRLGRNRLNVSIAQVERLGLGIMQEVARGFRSGARP
                     GFDVLAKSLDHPARSLEEITSRLEAAGLLTHTEEGHFVPGRDPQTITLAEVFAAVRGE
                     DAAGGDSGVTALIAQVRAAEAARLAGRTINDLAGDDRS"
     gene            complement(5546..6895)
                     /gene="pmbA"
                     /locus_tag="PLS34_05945"
     CDS             complement(5546..6895)
                     /gene="pmbA"
                     /locus_tag="PLS34_05945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HRP87046.1"
                     /translation="MSSADLSDLGGARARLQAMAGDALARAARNGASAAEVGASLATG
                     LSVTVRKGEVETLEHQRDRGFSVTVYVGRRKGSASTSDLAAAAIAETVDKACTIARHT
                     AEDPCAGLADAELMAREIPELELDHPWALDPDQAVSLARECEAAGLSADARITNTEGG
                     SLSSHRSLRVYANSHGFSGGYASTSHSLSCALIAGEGDEMQRDYWYTVARRAEDMESP
                     QSVGRRAAERALRRVGARRIGTRTAPVIYVAELARGFLGHLVSAVKGAAQYRKASFLL
                     GAAGSQVLPAWFDIEERPHLPRAPGSAPFDAEGVATHDRSLVKDGILQGYVLSSYSAR
                     RLGLQTTGNAGGIHNLLVAPGEEDLAGLIRRMGRGFVVTELMGQGINGVTGDYSRGAS
                     GFWVEGGEIVHPVQEITIAGNLKELYLDIQAAGADVDRRGVIQTGSLLIGRMTIAGD"
     gene            6994..8220
                     /locus_tag="PLS34_05950"
     CDS             6994..8220
                     /locus_tag="PLS34_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP87047.1"
                     /translation="MTRTTAATLDLDWVRARFAALDGDWALLDNAGGSAPLSAVAARI
                     SDYLHRWPVQLGASYAPSAAAGERQAEARRALAGLFANGGGRPPAPGRVAIGASTTSL
                     LSRLARSLAPTLAPGDEIVVTNADHEANIGAWLRLQARGVRIRWWRIRPDSMRLELED
                     LDALLSERTRLICFTHASNLLGSTLDLGPLVARARAAGALTCVDGVAFAPHRALAFEN
                     WGVDWYAFSLYKVFGPHCALLCSSEQGAGLLGNLNHEWMDPAEPATRLEPGAWPYELA
                     WGAAAVPEYLEALGVHHGAPAFELIAAHERALTRRLLDWLTTRPGIRIIGAAEVGADR
                     LPTVSFVAAGRCPAEIVQHADHARVGIRHGHFYAPRLVEALGLERASGVVRVSMAHYN
                     TAAEVDGLIRALEPVL"
     gene            8217..8738
                     /gene="yjgA"
                     /locus_tag="PLS34_05955"
     CDS             8217..8738
                     /gene="yjgA"
                     /locus_tag="PLS34_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HRP87048.1"
                     /translation="MRRDDEAPSKSQRKREAAALQSLGELLAELPAEQLDLLDLPDRL
                     RQALEQLRGIASHEARRRQRQFIGKLMRDLDPAPLQAFIDAQQRPSRESARLFRVTET
                     WRDRLVAEGDAAVRAFLATYPLTDAAALAEAVTAARQQRSGAPKRLFRMLRDIVERQA
                     VAHGSPGGATLLE"
     gene            8743..9243
                     /gene="purE"
                     /locus_tag="PLS34_05960"
     CDS             8743..9243
                     /gene="purE"
                     /locus_tag="PLS34_05960"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRP87049.1"
                     /translation="MNTLVGIIMGSRSDWSTMQHAAATLEALGVPFETRVVSAHRTPD
                     LLFEYCESARDRGLQVIIAGAGGAAHLPGMAAAKTSLPVLGVPVQSKALNGLDSLLSI
                     VQMPAGVPVATLAIGRAGAVNAALLAAAILGTGHPEILSALERNRGEQTRKVLADPDP
                     AAAAPE"
     gene            9240..>9634
                     /locus_tag="PLS34_05965"
     CDS             9240..>9634
                     /locus_tag="PLS34_05965"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRP87050.1"
                     /translation="MKIGIIGAGQLGRMLALAGYPLGQRFVFVDADPDAPGAQVGRII
                     HGRFDDPAALAALAASVDVVTFDVENVPQAAAEAVAAKVPFYPQPAALGAGQDRLDEK
                     RLFNALGIPTAPFTAVDSQQSLLRAVEQIG"
BASE COUNT         1409 a         3429 c         3286 g         1510 t
ORIGIN      
        1 tcaccggtac catggcggcc acgcggcggt tgctggcgcg catccccggg ctggcgacca
       61 gcgagttgcc ggccggctgt tgcggtgcgg ccggcgagaa tttcctcagc cggccgggga
      121 tggcggatgc gctgcttgcg ccgctgctcg acctgctgtc ggaggagccg cccgacgcgc
      181 tggtcaccag caacatcggc tgtgcgatgc acttcgctgc cggactgcgc cgtcgcggcc
      241 tcgaggtgcc ggtgctgcat cccgcctcgc tggtcgccga cgcgctacaa tcagccacat
      301 gagcgtacgc aggctgactc cgctggaccg ggtgctcgag caggtcgacc aggccgtgcg
      361 cacggtgctc ggccccccgc cccccgcctc gcgccccaac ccggccgagg gcgtcccgga
      421 tgcccccttg accgaccgcg agcgccgcca tgccgcgggg ctgatgcgag tcaaccacgc
      481 gggcgaggtc atggcccagg ggttgtacca gggccaggcg ctgaccgccc ggctggcgga
      541 agtgcgcggc gccatggaac aggccgccgt cgaggagttc gatcacctcg cgtggtgcga
      601 gcagcgcctc gccgagctgc gcgaccggcc cagcgtgctc ggaccggtct ggtacaccgg
      661 cgcttttttg ctgggcgcgg gtgccgggct ggccggcgac cgctggagcc tcggtttcgt
      721 ctcggagacc gaggcccagg tcatcaacca cctggaggat cacctggggc ggctgccgcg
      781 tgccgaccag cgcagccgcg cgatcatcgg ccagatgaag ctcgacgagg caaggcacgg
      841 cgctgaggcg cgcgcggccg gcggcctgcc gttgccggcg ccggcgcgcg ggctgatggc
      901 ggtcatgtcg aaggtcatga cccggacggc ctactgggtt tgaggagcgt gccggaacag
      961 gcctcaatct gctcggcggc ttgcccttgc aacgcctgtg ggttaaaagt gcacacgccg
     1021 cacggcttta cataagaaaa cagcgaggcc agcgaatggg tgagcccttg ggcagggaca
     1081 acgacatccc tgcaatgagt cgatttctcg agctgtgccg cgtccgctca gtgccctcca
     1141 agacggtcgt gattcatgcc ggcgacctgc cggacgtgct gtattacata gtcgacggct
     1201 cggtcgaggt catgatcgag gacgaggacg gcaacgagat ggtccttgcc tacctcaaca
     1261 agggacagtt cttcggcgag atgggcctgt tctacgagca gccgacgcgc agcgcctggg
     1321 tgcgcacccg caccgcctgc gagctcgccg agatgaccta cccccgcttc cggcaggtgg
     1381 cggcggaaaa cccggccttg ctgttcgaac tcgccacgca actggccacc cgcctcgagc
     1441 gcaccaaccg taagctcggc gacctggcct tcgtcgacgt cacgggccgt atcgcgcacg
     1501 ccatcatgga cctgtgttcc gagccggacg ccatgacgca cccggacggc atgcagatca
     1561 aggtcagccg gcaggagctg tcgcgcctgg tgggctgctc gcgcgagatg gccggccgcg
     1621 tgctcaaggt tctcgaggag cagggcctgc tgcgcgcgcg cggcaagacg atcgtcgtac
     1681 acggcgtccg ccccgagcac aagccgcgcc gcggctgatc aatcgccgcc gaggtcggcg
     1741 cctgccaact cgcggcgcat ggccgtgatg gtgctggcgt aatcgctggc gccgaagatc
     1801 gctgaccccg ctacgaaggt gtccgccccg gcgcgcgcaa tgtcggcgat gttgtccacc
     1861 ttgacgccgc cgtcgacctc gaggcgtatc ggcaggccgc tgtcatcgat gaactgccgt
     1921 gcctcggcaa tcttgcgcag cgccgtcggg atgaacttct ggccgccgaa accgggattg
     1981 actgacatga tgagcaccat gtcgatcttg tcggcgaggt aatcgagcca gctgagcggc
     2041 gtcgccgggt tgaacaccag cccggcccgg cagccgaggt cgcggatcag ctgcaggctg
     2101 cgatcgacgt gctcgctggc ttccgggtgg aaagtgatcc agctcgcccc ggcccgcgca
     2161 aaggactcga tcaatccgtc caccggcttg accatcaggt gcacgtcgat cggcgcctcg
     2221 atgccccagt tgcgcagcgc ctcgcagacc atcggcccga tggtcaggtt gggcacgtaa
     2281 tggttgtcca tcacgtcgaa gtggaccatg tcggcaccgg cggcgagcac cgcgtcgacc
     2341 tcctcgccga gccgcgcaaa atccgctgac aggatcgaag gggcaatgcg gtagtcgagc
     2401 atggcgctat cctaccgcat gccgcgcgcc gcccgaagcg tttcccaggc cgcacgaatc
     2461 tcgcgggtct ttgcttccgc aaccgggatc atcgactcgg gcatgccgcg cgctctcagc
     2521 ttgtccggat ggtgctgatt catcagccgg cgataggcca gcttgacctc ggcgtcgctg
     2581 gccgccggct cgataccgag cacccggtag gcgtccgccg cagtcgaccg cggctgcacc
     2641 tgccggtcgc cgcccgcagc acgcccgaaa cgcaagagcg cttcgacctg ggccagttcg
     2701 acccggccga ggtccagcgc cgaggcgatg cgccagagca tgtcccttgt ctcggcgccc
     2761 atgtagggat tggagagtgc ggcttccacc tggatctcca ggaacgcgcg gcgcagcgtg
     2821 cgccgcgaac ccagcgcatc cgcgaggcgg gcaagctcgc gctccatcgg ccagtcgggc
     2881 gccttgccga tgttgaacag gcggatggcc tcctggacct gcgtcgcatc cagccgcatc
     2941 cggctcatga tgccccgcgc cgcctggatc tcggcctcgc tgacccggcc gtccgccttg
     3001 gcgacatgcc ccatgacggc gaaggtggtc tcgaagaaca cctggccgac cccgtcgaaa
     3061 ctttcacgcc gcgcgccgcc gacgccgcga tcgaactggt gtccgagaaa cagcccgacg
     3121 gcggccccga ccgggccggc cagcgaaccg atgatcacgc ccactagctt gccggtccag
     3181 ttcatgcagg tgcgcaacca gagtgatcgg gacgacgtgc ccatttgacg ggcgccgcga
     3241 cccgctggga gaatccggaa gaccaaggcc tgagcctccc cggggagttc ccatgttacc
     3301 agcacgcgaa atcgccgcca gcatggccgg tgtgcatcgc ctgttgccgg tggcgcgatg
     3361 agcgccatgt ttcaatccga tcttccgggg ctcgaactcc tgcaccgggg caaggtgcgc
     3421 gacgtctacg cggtcgatga ccggacgctg ttgatcgtcg cgaccgaccg gctgtcggcc
     3481 ttcgacgtgg tgctgcccga tcccatcccg cacaagggcg agattctcac gcagacggca
     3541 ctgttctggt ttcaccggac tcgcgcgctg gtgcccaatc acctgctcga gcgcccgctc
     3601 gactcggtcc tgccgggggg cgtcgaccgg gacgactatc ggctgcgcag cagcgtgacg
     3661 cggcggttgc gcccgctgcc gatcgaggcg gtggcgcgcg gctacctgat cggctccgga
     3721 tggaaggatt accaggccgg gggttcggtg tgcggcgtgg cgctgccgcc cggcctgcgg
     3781 ttggccgacc gcctgcccga accgatcttc acgcccgcca ccaaggctgc cgccggggaa
     3841 catgatgaga acatcgccta cgagcaggtg gtggaactgg tcgggcccgg actggccgcg
     3901 caggtgcgcg acctgacctt gcggatttac ggcgaggccg cggcctatgc gcgtgagcgc
     3961 ggcatcatca tcgcggacac caagttcgaa ttcggtctcg acgagcacgg cgaactctat
     4021 ctcatcgacg agatcctgac gccggactct tcgcgtttct ggcccgcgga cgagtggcgg
     4081 cccggcgcga gcccgcccag cttcgacaag cagtacgtcc gtgactacct cgagacgctc
     4141 gcctgggaca agcaggcccc cggcccgcgc ctgcctgcgg aaatcatcgc gcgcactgcc
     4201 gacaagtatc gtgaggcgta ccgccgcctg accggcgacg acgagctgtt cggggcatga
     4261 gcgacacctt gcagagccgg gtcgagcgcc tgctgtggaa caaggaatcc gcggccgagc
     4321 cgccgctgcg ccgggcgctg cggctgcccg cgaggttcgt ctacgcgctg acgcgcgacc
     4381 tcgccgaggg ccagctgacg ttgcgcgcca tgagcctcgt ctacaccacg ctgctgtcgc
     4441 tggtgcccct gctcgccttc agcttctccg tcttgaaggg cttcggagta catcgccagg
     4501 tcgagcccct gctctacgag ttcctgctgc cgctcggcga acacggcgca cagatcaccg
     4561 accagatcat cgccttcgtg gagaatgtgc gtggcggcgt gctcggcggc atcggcctcg
     4621 cactgctcat gttcacggtg atctcgatga tccagaaggt ggaggacacc ttcaactaca
     4681 tctggcaggt gcagaaatcg cgcaacctgg cgcgtcgctt cagcgactat ctcagcgtga
     4741 tcctggtcgg tccggtgctc atggtgacgg cgatgggcct gctcgcgacc atcaattcca
     4801 gcgacatcat gcaggccatc gccgaggtcc agccctttgg cacgctgctg gccgtgatcg
     4861 gccgactggc gccgttcacg ctggtggtgg tggtgttcgc cttcgtctac gcgttcgtgc
     4921 ccaacacccg ggtccggctc ggcgcggcgc tggtcggggc ggtgctggcc ggcgccgcct
     4981 ggacccttgg cggctcgctg ttcgccacgg tgatcgtcgg ctcgacccgc tatgccgcca
     5041 tctattccag cttcgcgatt gcgattttcg cgctcatctg gctgtacctg tcgtggctgg
     5101 tcctgctgct cggtgcgcag gtggcgtttt acgtgcagaa cccgcggcgc ctgcgcctcg
     5161 gccgcaaccg cctcaacgtc agcatcgcgc aggtcgagcg cctgggactc ggcatcatgc
     5221 aggaggtcgc gcggggcttt cgcagcggcg cgcggcccgg cttcgacgta ctggcgaaat
     5281 ctctcgacca ccccgcgcgg tcgctcgagg agatcacttc gcgcctggag gccgccggcc
     5341 tgctgacgca caccgaggaa ggccatttcg tacccggccg cgacccgcag accatcacgc
     5401 tcgccgaggt gttcgctgca gtgcgcggcg aagatgcggc cggcggtgac agcggggtca
     5461 cggcgctgat cgcccaagtg cgcgcggcgg aggcggcccg gctggcaggt cgtaccatca
     5521 acgacctcgc gggcgatgat cgaagctagt cgccggcgat ggtcatccgg ccgatcagca
     5581 gcgagccggt ctggatgacg ccgcgccggt cgacgtccgc gcctgcggcc tggatgtcca
     5641 ggtagagctc cttgaggttg ccggcgatgg tgatttcctg cacggggtgc acgatctcgc
     5701 cgccctcgac ccagaagccg ctcgcgccgc gggaataatc cccggtgacg ccgttgatgc
     5761 cctgtcccat caattcggtg acgacgaagc cgcggcccat gcggcgaatc aggcccgcga
     5821 gatcttcctc gcccggggcg accagcaggt tgtggatgcc gccggcgttg ccggtcgtct
     5881 gcaagccgag ccggcgtgcc gaatagctcg acagcacata gccctgcagg atgccgtcct
     5941 tgaccaggct acgatcatga gtggccacgc cttcggcatc gaacggcgcg gaacccggtg
     6001 cccgcggcag gtgcggacgt tcctcgatgt cgaaccatgc cggcagcacc tggctgccgg
     6061 cggcccccag caggaagctc gccttgcggt actgcgcggc gcccttgacc gcggagacca
     6121 ggtgcccgag gaagccgcgc gccagctcgg cgacgtagat caccggcgcg gtgcgcgtgc
     6181 cgatgcgacg cgctccgacg cgtcgcaacg cccgctccgc cgcgcggcgg cccaccgatt
     6241 gcggcgactc catgtcttcg gcgcggcgcg ccaccgtgta ccagtagtcg cgctgcatct
     6301 cgtcgccctc accggcgatc agggcacagc tgagactgtg gctggtgctc gcgtagccgc
     6361 cggaaaagcc gtggctgttg gcatacaccc gcaaggagcg atgcgaggac agcgaaccac
     6421 cctcggtatt ggtgatgcgc gcatcggctg acaggccggc ggcctcgcac tcgcgcgcca
     6481 ggctgaccgc ctggtccgga tcgagcgccc agggatggtc gagctcgagt tccggaatct
     6541 cgcgcgccat cagttcggca tccgccagcc ccgcacaggg atcctcggcc gtatggcggg
     6601 cgatcgtgca ggccttgtcg accgtctcgg cgatcgcggc cgcagcgagg tcggaagtgc
     6661 ttgcgctgcc cttgcgccgg ccgacataca ccgtgacgct gaagccgcgg tcacgctggt
     6721 gctcgagcgt ctcgacctcg cccttgcgta ccgtcaccga cagtccggtg gcgaggctcg
     6781 cacccacctc ggcagcggat gcgccgttgc gcgccgcacg cgccagggcg tcgcccgcca
     6841 tggcctgcag gcgggcgcgc gcgccgccga ggtcgctcaa atctgccgat gacatcgatc
     6901 cgccttccgc cgcgtgcttc cagtccctgg tgagcggcgc agtgtagcaa acctccatgc
     6961 tatgatcgcg gccccctggc gcccggcaaa cgcgtgactc gaacgactgc tgcaacgctg
     7021 gacctggact gggtgcgcgc tcgtttcgcg gccctggacg gcgactgggc gctgctcgac
     7081 aacgccgggg gctcggcgcc gctgtccgcc gtcgccgcgc gcatctcgga ctacctgcac
     7141 cgttggcccg tgcagctcgg cgccagttat gccccgtcgg cggctgccgg cgagcgccag
     7201 gccgaggcgc gtcgtgcgct cgccgggctg ttcgccaacg gcggcggtcg cccgcccgcc
     7261 ccgggccgcg tcgccatcgg cgcctcgacg acctcgctgc tgtcgcgcct ggcgcgttcg
     7321 ctcgctccca ccctcgcgcc cggcgacgag atcgtcgtca cgaacgcgga ccacgaggcc
     7381 aacatcggcg cctggctgcg cctgcaggcc cggggcgtgc gcattcgctg gtggcggatc
     7441 aggcctgaca gcatgcgcct cgagctcgag gacctggacg ccctgttgtc cgagcggacg
     7501 cggctgatct gcttcaccca cgcctccaac ctgctcggca gcacgctcga cctgggaccg
     7561 ctggtagcgc gcgcccgggc cgccggcgca ctgacctgcg tggatggcgt cgccttcgcg
     7621 ccccaccgcg cgctggcctt cgagaactgg ggcgtcgact ggtacgcatt cagcctctac
     7681 aaggtgttcg gcccgcattg cgccctgctc tgcagctcgg agcaaggcgc cggcctgctc
     7741 ggcaacctca atcacgagtg gatggacccc gccgagccgg ccaccaggct cgagccgggc
     7801 gcctggccgt acgagctggc atggggcgcg gcggcggtgc cggaatacct cgaagcactc
     7861 ggcgtgcatc acggcgctcc ggccttcgag ctgatcgcgg cgcacgagcg cgcgctcacc
     7921 cgccggctgc tcgactggct cacgacgcgg cccggcatcc ggatcatcgg cgccgcggag
     7981 gtcggtgccg acaggttgcc gaccgtttcg ttcgtggctg ccggccgctg ccccgcagag
     8041 atcgtgcagc atgccgacca cgccagggtg ggaatccgtc atggccattt ctatgcgccg
     8101 cgcctggtcg aggcactcgg gctggaacgc gccagcggcg tggtccgcgt gtcgatggcg
     8161 cactacaaca cggctgccga ggtcgacggc ctgatccgcg cactggagcc ggtgctgtga
     8221 ggcgcgacga cgaggccccc agcaagtccc agcgcaagcg tgaggccgcg gcgctgcagt
     8281 cgctcggcga actgctcgcc gagctgcccg ccgagcagct cgatctgctc gatttgcccg
     8341 accggctgcg ccaggcgctg gagcagctgc gcggcatcgc ctcgcacgaa gcccggcgcc
     8401 gccagcggca gttcatcggc aagctgatgc gggacctgga cccggcgccg ctgcaggcct
     8461 tcatcgacgc gcagcagcgg ccgagccgcg aatccgcccg gctgtttcgc gtcaccgaga
     8521 cctggcgcga ccggctggtc gcggagggcg atgccgctgt ccgggccttt ctggccactt
     8581 accccctcac ggatgcggcg gcgctggcag aagcggtgac ggcggcccgc cagcagcgca
     8641 gcggcgcccc gaaacgactg ttccggatgc tgcgtgacat cgtcgagcga caggcggtgg
     8701 cccacggctc gcccggcggc gcgacgctgc tagaatagcg cgatgaacac gctcgtcggc
     8761 atcatcatgg gatcgcgctc cgactggagc acgatgcagc acgcggccgc cacgctggag
     8821 gcgctcggcg tgcctttcga aacgcgagtg gtgtcggcgc accgcactcc ggacctgctg
     8881 ttcgagtact gcgagtcggc ccgcgaccgg ggcctccagg tcatcatcgc cggcgccggc
     8941 ggcgcggcac acctgccggg catggcggct gccaagacca gcctgccggt gctcggcgtg
     9001 ccggtgcagt ccaaggcgct gaatggcctc gactccctgc tgtcgatcgt gcagatgccg
     9061 gccggcgttc ccgtggcgac gctggccatc ggtcgtgcag gtgccgtcaa cgccgcgctg
     9121 ctggcggcgg cgatcctcgg cacgggccac cccgagattc tttcggcgct cgagcgcaat
     9181 cgcggcgagc agacccgcaa agtgctggcc gaccccgacc cggcggccgc agcccccgaa
     9241 tgaagatcgg catcatcggc gccggccagc ttgggcgcat gctggccctc gccggctatc
     9301 cgctcggcca gcgcttcgtg ttcgtcgatg ccgacccgga tgcacccggc gcccaggtcg
     9361 gccgaatcat ccacggccgc ttcgacgacc cggccgcgct ggccgcgctg gccgcctccg
     9421 tggacgtcgt gactttcgac gtggagaacg taccgcaggc ggccgccgag gccgtggccg
     9481 ccaaggtgcc gttctatcca cagcccgcgg cgctcggcgc cggccaggac cggctcgacg
     9541 agaagcgcct gttcaatgct ctgggcatcc cgacggcgcc gttcacagcg gtggactcgc
     9601 agcagtcgct gctgcgtgcc gtcgagcaga tcgg
//
LOCUS       DAOMPG010000080         9615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3058_length_9615_cov_5.001883, whole genome shotgun sequence.
ACCESSION   DAOMPG010000080 DAOMPG010000000
VERSION     DAOMPG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3058_length_9615_cov_5.001883"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /gene="glnA"
                     /locus_tag="PLS34_05970"
     CDS             <1..1376
                     /gene="glnA"
                     /locus_tag="PLS34_05970"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HRP87051.1"
                     /translation="KEVAFVDLRFTDTKGKEQHVTVPASKVDADLFEEGKMFDGSSIS
                     GWKSINESDMILMPDPSTAVVDPFSEDTTVNITCDVVEPSTMQGYERDPRSLARRAEA
                     YLKSTGIADVAYFGPENEFFIFDDVRWSSEMKGVSYQIDSSEAGWNSEKIYPDGNFGH
                     RPTIKGGYFPVPPVDSLHDIRSAMCLVLADMGLDPEVHHHEVATAGQCEIGVAFGSLV
                     KKADEVQKLKYAVWNVAQGYGKTATFMPKPLVGDNGSGMHVHQSLAKDGKNLFTGEEY
                     GGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVKLAYSARNRSASIRI
                     PYIPNPKGRRIEVRFPDSTANPYFAFAAMMMAGLDGIQNRIHPGDPMDKDLYDLPAEE
                     SKSIPEVCYSLEEALNALDADRGFLTAGGVFTDDLINAYIKLKMADVTRLRMTTHPVE
                     FDMYYSV"
     gene            1491..2048
                     /locus_tag="PLS34_05975"
     CDS             1491..2048
                     /locus_tag="PLS34_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRP87052.1"
                     /translation="MRWISVALLLMVCAGAAASEMWRWVDERGVVHFSDRPHPGAERV
                     DVKPAQSYQAPPVASPPETVDTPPLSRQEPASSYTRLAILAPAAGETLWNIGGQLGVE
                     LAIEPPLAAGHELRVYLDEQRVADELRGTQFTLSEVYRGEHRLRASIVDARGRELVSS
                     ETVVFYVQQASLQNPNRPSPRPGGG"
     gene            2038..3120
                     /gene="glnL"
                     /locus_tag="PLS34_05980"
     CDS             2038..3120
                     /gene="glnL"
                     /locus_tag="PLS34_05980"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HRP87053.1"
                     /translation="MAAERSVTQPRGEYLENLTTGVVVLGAALEVRYANPAAEQLLGV
                     SRQQLGGRVLTEVVPGLGMLRPLLEKVRDTGQGLSNREVLVADRPDGEPGLALDCALT
                     TFDGAAGSRALLLEIADASRRLRIDREKQLLAQLEASRSMVRQLAHELRNPLGGIRGA
                     AQLLERQLDSPAQQDYTNLIIREADRLVGLTTALLGPSHAPRKNLLNIHEQLEHVRRL
                     IVAEGGPGIQVERDYDPSLPPTLLDRDLVVQAMLNLARNALAALDGRGRIIFRTRVVS
                     NATIGHRRYRLAACVEIEDDGPGVPEELRETLFYPLVTGRPGGSGLGLAVAQELVSRH
                     DGLIEYTSRPGRTVFRMLFPIREDDG"
     gene            3132..4512
                     /gene="ntrC"
                     /locus_tag="PLS34_05985"
                     /pseudo
     CDS             3132..4512
                     /gene="ntrC"
                     /locus_tag="PLS34_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627771.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
     gene            complement(4565..5152)
                     /locus_tag="PLS34_05990"
     CDS             complement(4565..5152)
                     /locus_tag="PLS34_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRP87054.1"
                     /translation="MSSIAELHPVILTLAIFLARILDVSLGTVRTIVVFRGHRLLAAV
                     IGFFEIVIWVIAAGQVLQNLDAWYLIVAYAGGFGAGNYIGIWLEAKLAIGREMVSAIS
                     FRADGELAKLLRERGFRSIDVDADMGRGPAVDLVITVTRRRRVPELLRTILEADPEAQ
                     YSISDIKMAHEGFDPTMITPRASYSGGWRSIMKRK"
     gene            complement(5197..6174)
                     /locus_tag="PLS34_05995"
     CDS             complement(5197..6174)
                     /locus_tag="PLS34_05995"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRP87055.1"
                     /translation="MTGKTDHNPLALDFCGMRFDSPIVLLSGCVGFGEEYTRVAGFSN
                     RDAGAVVLKGTTGAARLGNPPHRVYETPQGMLNAIGLQNPGAAKVVRDILPTLDFSET
                     RFLANVSGSTIEEYVEVTRLFDDSPIDGIEINISCPNVKEGGVAFGNYPDMSAKVVEA
                     CRRVTSKPLITKLSPNQTDIQENARRCIEAGTDGFSVINTVMGMAVDAERREPVIGNV
                     QGGLSGPAIKPIALLKVHQVYQVARPHGVPIIGQGGIVSARDALEFIIAGAAAVGVGT
                     ALFYDPLACRKINDGIREYLGRHGFASVGELVGTLRLPGEAPAPRAAAR"
     gene            6195..6671
                     /locus_tag="PLS34_06000"
     CDS             6195..6671
                     /locus_tag="PLS34_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRP87056.1"
                     /translation="MFHVILWQPEIPPNTGNIIRLCANTGATLSLVRPLGFALDEPRL
                     RRAGLDYHEFAVVREFDDLGACLASLGRPRVWAFSARATQSYAAVEYAHGDALLFGPE
                     TRGLPAAALAMVPASQQLVIPMRAGNRSLNLSNAVAVAVYEAWRQQGFSRPAQTPT"
     gene            complement(6643..7668)
                     /locus_tag="PLS34_06005"
     CDS             complement(6643..7668)
                     /locus_tag="PLS34_06005"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012517045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRP87057.1"
                     /translation="MSGSAPGEAIAVLGAGSWGTALAIQFARSGRPALLWGRAEDDPA
                     GMEQARQNARYLPGAKFPPSLHAEADLDKVLAAAPIVVVAVPSHAFRAVLTQMRPLMR
                     PGTRLAWATKGFELETGKLPHQVAREVLGSGTAMAVLSGPTFAREVGMGLPTAMTIAA
                     NDEDYARQLAENLSGESFRAYMSSDIVGVEVGGAVKNVLAIGAGICDGLGFGANTRVA
                     LITRGLVEMTRLGVALGAQPATFMGLAGMGDLVLTCTDDQSRNRRMGLALAAGKTVQE
                     AEREIQQVVEGVLAAEAVHRVAARAGVEMPICEQIYRILYEGAEPKEAVVALMKRELK
                     SEFAPGG"
     gene            complement(7676..8188)
                     /gene="secB"
                     /locus_tag="PLS34_06010"
     CDS             complement(7676..8188)
                     /gene="secB"
                     /locus_tag="PLS34_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRP87058.1"
                     /translation="MAEQRTDGIDANPVTVALQRVYLKDSSFEAPNSPSIFQGEWRPS
                     VSLNIGTSTTELGDGAYEAVLSLTVEAKQGDKTAFLIEAQQAGIFVLQGLAPNDLQRA
                     LMTFCPQQLYPFAREVISDLSSKGGFPALQLQPVNFDAVAAQAMRKQQEEMAAQAAST
                     ASGAAPGSGH"
     gene            complement(8226..8501)
                     /gene="grxC"
                     /locus_tag="PLS34_06015"
     CDS             complement(8226..8501)
                     /gene="grxC"
                     /locus_tag="PLS34_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRP87059.1"
                     /translation="MTAPVQVYVSRYCGWCTRALALLEAKGAAPEVISVDSDRELRGE
                     KELRSGRRTVPQIFIGDRHVGGFDDLRALETAGQLDAMLREEQAAAS"
     gene            complement(8498..8896)
                     /locus_tag="PLS34_06020"
     CDS             complement(8498..8896)
                     /locus_tag="PLS34_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRP87060.1"
                     /translation="MLLMFAVVFAYEMRVARRKGTDVSAPEAVAMINAGAQPVDIRAA
                     AQYEKAHILDARNIPLGDLDQHLASLEKLKDRGILLYCDNGAASAKAVETLAARGVPG
                     AKSLRGGLTAWRGENLPVFSGRKSRKKDAS"
     gene            9041..>9615
                     /locus_tag="PLS34_06025"
     CDS             9041..>9615
                     /locus_tag="PLS34_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87061.1"
                     /translation="MRRPIRAALLVFLILLAGGGAAGASARDAELARQLEELKARIAV
                     IEKAQREAADRRDRESRALREAELAVARQSRTLEETRRERQAVAARRDDLQARRTEAA
                     AGLEADTEALAAELRAAWMAGGEPRLKLILNQGDPAELGRMLTWYGYVARERAVRVAS
                     LRERLAELAELGRRLDAENARLEAAEARLAVA"
BASE COUNT         1445 a         3345 c         3362 g         1463 t
ORIGIN      
        1 aaaaggaggt cgccttcgtg gacctgcgct tcaccgacac caagggcaag gagcaacacg
       61 tgaccgtgcc cgcgagcaag gtcgacgccg acctgttcga ggaaggcaag atgttcgacg
      121 gctcgtcgat ctcggggtgg aagagcatca acgagtccga catgatcctg atgcccgatc
      181 cgtccacggc cgtggtcgac cccttctccg aggacaccac ggtgaacatc acctgcgacg
      241 tggtggagcc ctccaccatg cagggctacg agcgtgaccc acgctccctg gcgcggcgcg
      301 ccgaggccta cctgaagtcg accggcatcg ccgacgtcgc ctacttcggc ccggaaaacg
      361 agttcttcat tttcgacgac gtccgctgga gctcggagat gaagggcgtc tcctaccaga
      421 tcgactcgtc cgaggccggc tggaactcgg agaagatcta cccggacggc aacttcggcc
      481 accgcccgac catcaagggc ggctacttcc ccgtgccgcc ggtcgactcg ctgcacgaca
      541 tccgctcggc catgtgcctg gtgctggccg acatgggcct tgacccggaa gtgcaccacc
      601 acgaggtcgc gaccgccggc cagtgcgaga tcggcgtggc cttcggctcg ctggtgaaga
      661 aggccgacga ggtgcagaag ctgaagtacg cggtgtggaa cgtggcccag ggctacggca
      721 agacggccac cttcatgccg aagccgctgg tcggcgacaa cggcagcggc atgcacgtcc
      781 accagtcgct cgccaaggac ggcaagaacc tgttcacggg cgaggagtac ggcggcctgt
      841 ccgagaccgc gctctactac atcggcggca tcatcaagca cgcgcgcgcg ctgaacgcct
      901 tcaccaatcc ctcgaccaac agctacaagc gcctggtgcc gggattcgag gcgcccgtga
      961 agctggccta ctccgcccgc aaccgctcgg cctccattcg cattccctac attcccaacc
     1021 cgaaggggcg ccgcatcgag gtccgcttcc cggactcgac cgccaacccc tatttcgcgt
     1081 tcgcggcgat gatgatggcc ggcctcgacg gcatccagaa ccgcatccat ccgggcgacc
     1141 cgatggacaa ggacctctac gacctgccgg ccgaggagtc caagagcatt cccgaggtct
     1201 gctactccct cgaggaggcg ctcaatgcgc tcgacgccga ccgcggcttc ctcacggccg
     1261 gcggggtgtt cacggacgac ctgatcaatg cctacatcaa gctcaagatg gcggatgtga
     1321 cgcggctgcg catgaccacg catcccgtcg aattcgacat gtactacagc gtctgacgag
     1381 tctggaggac gcgtttcggg cggcgggttc gatcccgccg cccgcttttt tgtcagccgg
     1441 gctcgccgcc cgctatgcta ggcgccctgc tggtctcggg aggcaatccc atgcgctgga
     1501 tatcggtggc actgctgttg atggtctgcg ccggcgcggc ggcgagcgag atgtggcgct
     1561 gggtggacga gcgcggcgtg gtgcacttct ccgaccgccc gcacccgggc gcggagcgcg
     1621 tcgacgtcaa gccggcgcaa tcgtaccagg cgccgcccgt cgccagtccg cccgaaacgg
     1681 tcgacacccc gcccctttcc cggcaggagc ctgcaagctc ctacacgcgc ctcgccatcc
     1741 tggcgccggc cgcgggcgag accctgtgga acatcggtgg ccagctgggc gtcgagctgg
     1801 caatcgagcc gccgctggcc gccgggcacg aactgcgggt ctacctcgat gagcagcgcg
     1861 tggcggacga gttgcgcggc acccagttca ccctcagcga ggtctatcgg ggcgagcacc
     1921 gcctgcgcgc ctcgatcgtg gatgcgcgcg ggcgtgaact ggtgtccagc gaaacggtcg
     1981 tcttctatgt ccagcaggcc tcgctccaga accccaacag gccttcgccc cgccccggtg
     2041 gcggctgagc gcagcgtcac gcagccgcgc ggcgaatacc tggagaacct caccacgggc
     2101 gtcgtcgtgc tcggcgccgc gctggaggtc cggtacgcca atcctgcggc cgaacagctg
     2161 ctcggcgtca gccgccagca gctgggcggg cgcgtgctga cggaggtggt gccggggctc
     2221 gggatgttgc ggccgctgct ggagaaagtg cgcgacaccg gccagggcct gtcgaaccgc
     2281 gaggtcctgg tggccgaccg cccggacggc gaaccagggc tggcgctgga ctgcgcgttg
     2341 actaccttcg acggcgccgc gggcagccgt gcgctgctgc tggagattgc cgacgccagc
     2401 aggcgcctgc gcatcgatcg ggaaaagcag ctgctcgccc agctggaagc gagtcgcagc
     2461 atggtgcgcc agctggccca cgagctgcgc aatccgctgg ggggcatccg cggcgccgcc
     2521 cagttgctgg aacgccagct cgattccccc gcgcagcagg actacacgaa cctgatcatt
     2581 cgtgaggccg accgcctggt cgggctgacc accgccctgc tgggcccgtc ccatgcgccg
     2641 cgcaagaacc tgctgaacat ccacgagcag ctggagcacg tccgccggct gatcgttgcg
     2701 gaaggcgggc cagggatcca ggtcgagcgc gattacgatc ccagcctgcc gcccaccctg
     2761 ctcgaccgcg acctggtcgt gcaggccatg ctcaacctgg cgcgcaatgc gctcgccgcc
     2821 ctcgacggcc gtggccgcat catctttcgg acgcgcgtgg tcagcaacgc caccatcggc
     2881 caccgccgct atcggctggc cgcgtgcgtc gaaatcgagg acgacggtcc cggtgttccg
     2941 gaggagctgc gcgagacgct gttctatccg ctggtcaccg ggcggcccgg cggcagcggc
     3001 ctgggcctgg cggtggcgca ggaactggtg agccggcatg acgggctgat cgaatacacc
     3061 agccggcccg gccggacggt gttccgaatg ctgtttccga tcagggagga cgacgggtga
     3121 cgcacgatcc catcgaggtc tggatcgtgg acgacgacgc cggcatccgc tgggtgctgg
     3181 agcgcgccat ggagcaggcc ggcatggcgt cccggggctt cgacagcgcg cccccggccc
     3241 gacgcactgc tgcacggcgc gcccgacgtc ctggtaaccg acatccgcat gccgggaccc
     3301 gacggtctcg acctgctggt gcgccttaag tccgactggc ccgagctgcc ggtgatcgtg
     3361 atcacggcgc actccgacct ggaaagcgcg gtcgccgcct accagggcgg cgccttcgag
     3421 tatctcccca agcccttcga catcgaccag gcggtggagc tggtgcggcg cgccggcgcg
     3481 gaccgggtgg cgtcgcgcga ggaaccgccg cggccggcgc tgcccagcct ggtcggccag
     3541 gccgcggcca tgcaggacgt gtttcgcgcc atcggccgcc tctccggctc gagcatgacc
     3601 gtgctgatca ccggcgagtc cggcaccggc aaggagctgg tggcgcgtgc cctccaccag
     3661 cacagcccgc gcgccgcgcg gcccttcgtc gctctcaaca ccgccgcgat cgccgcggaa
     3721 ctgctcgagt cggaactgtt cggtcacgag aagggcgcct ttacgggcgc ggaggcgcgg
     3781 cgcgccggcc gcttcgagca ggcggacggc ggcaccttgt tcctcgacga gatcggcgac
     3841 atgtccccgg cgctgcagac ccgcctgctg cgcgtgctgg ccgagggcga gttctaccgc
     3901 gtcggcggcc aggtgccggt gcgcgtcgac gtccgggtga tcgccgccac caaccaggat
     3961 ctcgccaagg gggtcgagga ggggcgtttc cgcgaggacc tgttccaccg gctgaacgtc
     4021 atgcgcatcc gcatcccgcc cctgcgggag cgccgggagg acatcccgct gctgctgacg
     4081 cattacctgg gcaaggcggc cgacgaactc gcggtgccgg ccaaggcgct gtcccccgaa
     4141 gtgctggcat gcttcgtcgc cttcgactgg cccggcaatg tgcgccaact ggtgaacacc
     4201 tgccggcggc taacggtcgt ggcgcccggc cgtgagatcc gcgtttcgga cctgccgcag
     4261 gaattcggcg ccggctcgcc ggcgccccgg acgcagggct ggaccgacgc cctcgccgca
     4321 tgggccgggc agcagctgga tgccggcgac gagccattgc tgccggccgc gctggcggag
     4381 ttcgagcgga cgctgatcat cgtggccatg gcgcgcacgg acgggcaccg ccaggaagcc
     4441 gcgcgcctgc tcggctgggg acgcaacacg ctcgcccgca agatccgcga gctcgggctc
     4501 gacgagccgt aggcgccgcg gcgccggtgc ggggcgcgcc tcgacgggcg cgcccggcgc
     4561 tgtcctactt ccgcttcatg atggaccgcc agccgccgct gtagctggcg cgcggcgtga
     4621 tcatggtcgg atcgaagccc tcgtgcgcca tcttgatgtc gctgatcgag tattgcgcct
     4681 cggggtccgc ctcgaggatg gtgcgcagca gctccggcac ccgccgccgc cgggtcacgg
     4741 tgatgactag gtccaccgcg gggccgcggc ccatgtcggc gtcgacgtcg atggaccgga
     4801 agccccgttc gcgcagcagc ttggccagct cgccgtcggc acggaaggaa atggcgctga
     4861 ccatctcgcg gccgatggcc agcttcgcct caagccagat gccgatgtag ttgccggcgc
     4921 cgaagccgcc tgcgtaggcg acgatgaggt accaggcgtc gaggttctgc agcacctgtc
     4981 cggcggcgat cacccagatg acgatctcga agaagccgat cacggccgcc agcaggcgat
     5041 ggccgcggaa cacgacgatg gtgcgcaccg tgccgagcga tacgtcgaga atgcgggcca
     5101 ggaagatcgc cagggtgaga atcacgggat gcagttcggc aatcgagctc atcgtcgggc
     5161 tccgggatgt cgaattcggg aggaaacggc agcgggctag cgggcggctg cacgaggcgc
     5221 cggcgcttcg cccggcaggc gcagcgtgcc caccagctcg ccgaccgagg cgaagccgtg
     5281 ccggccgagg tattcgcgga tgccgtcgtt gatcttgcgg cacgccagcg ggtcgtagaa
     5341 cagcgccgtg ccgacgccga ccgccgcggc gcctgcgatg atgaactcga gcgcgtcacg
     5401 cgcgctgacg atgccgccct gcccgatgat cggcacaccg tgcggcctgg ccacctggta
     5461 gacctggtgc accttcaaca gggcgatcgg cttgatggcg ggccccgaca agccgccctg
     5521 gacattgccg atcaccggct cgcgccgctc ggcatcgacg gccatgccca tcaccgtgtt
     5581 gatcacggag aatccgtcgg tgcccgcctc gatgcagcgc cgtgcattct cctggatgtc
     5641 cgtctggttc ggcgacagct tggtgatgag cggcttcgag gtgacccggc ggcaggcctc
     5701 gaccaccttg gccgacatgt cgggataatt gccgaaggcg acgccgcctt ccttcacgtt
     5761 cgggcaggag atgttgatct cgatgccgtc gatgggcgag tcgtcgaaca gccgcgtgac
     5821 ctcgacgtat tcctcgatgg tggagccgct gacgttggcg agaaaacggg tctcggagaa
     5881 gtccagcgtc ggcaggatgt cgcgcaccac cttcgcggcg ccgggattct gcagcccgat
     5941 ggcgttgagc atcccctggg gcgtctcgta cacgcgatgt ggcgggttgc cgaggcgtgc
     6001 ggcgccggtg gtgcccttca gcacgacggc gcccgcgtcg cgattggaga agcccgcgac
     6061 ccgcgtgtac tcttcgccga atcccacgca gccggacagc agcacgatgg gcgagtcgaa
     6121 gcgcattccg cagaagtcca gcgcgagggg attgtggtcg gtcttgcccg tcatgcctgc
     6181 tccgttcagc tgaaatgttc cacgtgattc tctggcagcc ggagatccca ccgaacaccg
     6241 gcaatatcat acgcctgtgc gcgaataccg gcgccacgct gtcgctggtc cggccgctcg
     6301 gctttgcgct ggacgagccg cggctgcggc gggcggggct cgattaccat gagttcgcgg
     6361 tggtccgcga gttcgacgat ctgggcgcct gcctggcttc gctgggccgg ccgcgcgtgt
     6421 gggccttttc ggcgcgcgcg acccaaagct atgcggcggt ggagtatgct cacggcgacg
     6481 ccctgctgtt cggccccgag acgcgtggcc tgccggcggc ggccctggcg atggtgccgg
     6541 cgtcgcagca gctggtgatc cccatgcgcg ccggcaacag gagcctgaat ctctccaatg
     6601 cggtggcggt ggcggtgtac gaggcgtggc ggcagcaggg cttcagccgc ccggcgcaaa
     6661 ctccgacttg agctcgcgtt tcatcagcgc caccaccgct tccttcggct ccgcgccctc
     6721 gtagaggatg cggtagatct gctcgcagat gggcatctcg acaccggcac gtgccgccac
     6781 ccggtgcacg gcctccgccg ccagcacgcc ctcgacgacc tgctggatct cccgctcggc
     6841 ctcctggacg gtcttgccgg ccgcgagcgc gagccccata cgccggttgc gcgactggtc
     6901 gtcggtacag gtcagcacga gatcgcccat gccggccagc cccatgaaag tcgccggctg
     6961 tgcgccaagc gcgaccccga ggcgggtcat ctccaccagg ccgcgggtga tcagcgccac
     7021 gcgggtgttc gcgccgaagc cgagcccgtc gcagatgcct gcgccgatcg ccagcacgtt
     7081 cttcaccgcg ccgccgactt cgacgccaac gatgtcgctg gacatgtagg cgcggaagga
     7141 ctcgccggag aggttctcgg ccagctgccg ggcgtagtcc tcgtcgttgg cggcgatggt
     7201 catcgccgtc ggcaggccca tgccgacctc gcgcgcaaag gtcggcccgg acaggacggc
     7261 cattgcggtg ccggacccga gcacttcgcg cgccacctgg tgcgggagct tgccggtctc
     7321 gagctcgaag cccttggtcg cccaggccag gcgcgtgccc ggccgcatca gcggccgcat
     7381 ctgcgtgagc acggcacgga aggcgtggct cggcacggcc acgacgacga tcggcgccgc
     7441 cgccagcacc ttgtcgaggt ccgcttcggc gtggaggctg ggcgggaact tcgcgcccgg
     7501 caggtagcgc gcgttctggc gcgcctgctc catgcccgca gggtcgtcct cggcgcgccc
     7561 ccacagcagg gcgggccgcc cgctgcgcgc gaactggatg gcgagcgcag tgccccagga
     7621 gcctgcgccc agcaccgcga tcgcttcgcc cggcgccgag ccgctcatcg cggtgtcagt
     7681 ggcccgagcc gggcgctgcc ccggaggccg tggacgccgc ctgcgcggcc atctcttcct
     7741 gctgcttgcg catcgcctgc gcggcgacgg cgtcgaagtt caccggctgc agctgcagcg
     7801 ccgggaagcc gcccttgctg gagaggtcgg aaatgacctc gcgggcgaag ggatacagtt
     7861 gctgcgggca gaaagtcatc agcgcgcgct gcaggtcatt gggcgcgagg ccctgcagca
     7921 cgaagatgcc cgcctgctgg gcctcgatga ggaacgcagt cttgtcgcct tgcttcgcct
     7981 cgacggtcag gctgaggacg gcctcgtagg cgccgtcgcc gagctcggtg gtcgaagtgc
     8041 cgatgttgag cgacacggac gggcgccact cgccctggaa gatcgacggg ctgttgggcg
     8101 cctcgaacga gctgtccttg aggtatacgc gctgcagcgc cacggtgacg gggttggcat
     8161 cgatgccgtc ggttctctgc tcggccatga aagctcctgg atacgggtca gtttcgttgt
     8221 cagatctatg aagcggccgc ctgctcctcg cggagcatgg cgtccagttg tccggccgtc
     8281 tcgagggcgc ggaggtcgtc gaatccgccg acgtggcggt cgccgatgaa tatctggggt
     8341 acggtgcgcc gcccgctgcg cagttccttc tcgccacgca gttcgcggtc cgagtccacg
     8401 ctgatcacct cgggcgcggc gcccttggct tcgagcagcg ccagcgcccg cgtgcaccag
     8461 ccgcagtagc gggacacgta cacctgcacc ggcgcggtca cgacgcgtcc ttcttgcggc
     8521 tcttgcggcc cgagaatacc ggcaggttct ccccccgcca ggccgtgagg ccgccgcgca
     8581 ggctcttggc gcccggcacg ccgcgcgccg cgagcgtctc gaccgccttc gccgacgccg
     8641 cgccgttgtc gcaatagagg aggatgccgc ggtccttgag cttctccagc gaggcaaggt
     8701 gctgatcgag gtcgcccagc gggatgttgc gcgcatcgag gatgtgcgct ttttcatact
     8761 gcgcggcggc gcgaatgtcg acgggctgcg cgccggcatt gatcatcgcg accgcctcgg
     8821 gtgccgagac gtccgtcccc ttgcggcgcg cgactcgcat ttcgtaggcg aacaccaccg
     8881 cgaacatcag cagcatgccg ccgaacagga aggggtggct ggcggcgtat tggagtatcg
     8941 gttccatcgg ggccctgtcc acggacacaa gcgggtaatt ataaccgctc ccgcccgcgg
     9001 tcttgcccgt tttcgcgcat cgcctacact ccgccccacc gtgagacgtc cgattcgcgc
     9061 cgccctgttg gtatttctga tcctgctggc cggaggcggc gcggccgggg cgagcgcgcg
     9121 cgatgcggag ctggcccggc agctcgagga gctgaaagcc aggatcgcgg tcatcgagaa
     9181 ggcgcagcgc gaggccgcgg accgcaggga ccgggaatcg cgggccctgc gcgaggccga
     9241 gctggcggtg gcgcgccagt cgcggacgct ggaggagacc cggcgcgagc ggcaggccgt
     9301 ggcggcgcgc cgcgacgatt tgcaggcgcg gcggacggaa gccgcggccg ggctggaggc
     9361 cgacacggag gcactggccg ccgagctgcg cgccgcctgg atggccggcg gcgagccgcg
     9421 gctgaagctc atcctgaacc agggcgatcc cgcggagctg ggccggatgc tcacctggta
     9481 cggctacgtg gcccgcgagc gcgccgtccg ggtggcatcg ctgcgcgagc gtctggcgga
     9541 gctggccgag ctcgggcggc gcctggacgc cgagaacgcc cgcctggagg cagccgaggc
     9601 gcgcctcgcc gtggc
//
LOCUS       DAOMPG010000081         9597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3066_length_9597_cov_3.704779, whole genome shotgun sequence.
ACCESSION   DAOMPG010000081 DAOMPG010000000
VERSION     DAOMPG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_9597_cov_3.704779"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="tilS"
                     /locus_tag="PLS34_06030"
     CDS             complement(<1..718)
                     /gene="tilS"
                     /locus_tag="PLS34_06030"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRP87062.1"
                     /translation="MNSFSVEELRQRLEAAAGGAGGAASRRYCVAFSGGLDSTVLLVA
                     MARLGARAALGGLRAIHVHHGLHADANRWAADCEVRCRELGVPLEVVAVDARAAPGES
                     PEARARELRYQALAARLAPGEVLLTAQHADDQLETVLIQLLRGAGPAGLAAMPADTVF
                     GVGRHQRPLLGFTRASLEAWARAEGLAGWLEDPANRAPQLSRNHLRHEVLPAVRAHWP
                     GAAAAVSRAARHCAEAAGLLD"
     gene            complement(729..1268)
                     /locus_tag="PLS34_06035"
     CDS             complement(729..1268)
                     /locus_tag="PLS34_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87063.1"
                     /translation="MKPLISMLVRVVAVAGVAGLSMSAALAFDGPLHGAVLAGINDHG
                     NLVLVLDRYQGPGDAENGEADAVIMFAAADDAPLLEIVEAIRRSESRVNLDMQRDREG
                     AFLLLNIPRSAEYLLAVDYGRAVPNDRAGSMHNPVRPGATAIAGLALSIVHTGPMGWT
                     LREALDHYQSFELVEQPPG"
     gene            complement(1540..2499)
                     /locus_tag="PLS34_06040"
     CDS             complement(1540..2499)
                     /locus_tag="PLS34_06040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRP87064.1"
                     /translation="MNLNFLDFEQPIAELEAKIDELRFVGDDSQVNLSSEIRRLKSKS
                     EALTRNIFSSLTAWQVAQVARHPQRPYTLDYVERLFTDFEELHGDRTYADDAAIVGGL
                     ARFEGRPVMIVGQQKGRDTKAKVQRNFGMPRPEGYRKALRLMKLAEKFGLPVITFIDT
                     PGAYPGIGAEERGQSEAIARNLLEMAGLRTPIIGVVIGEGGSGGALAIGVADHILMLQ
                     YSIYSVISPEGCASILWKSADKSADAAEAMGITAEKLFEQGLVDEVVPEPLGGAHRDF
                     DAMAETLRGAIARYLGRLDKLPMDQLLTTRYQKLVSTGRFREG"
     gene            complement(2580..6087)
                     /gene="dnaE"
                     /locus_tag="PLS34_06045"
                     /pseudo
     CDS             complement(2580..6087)
                     /gene="dnaE"
                     /locus_tag="PLS34_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017252789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            complement(6084..6689)
                     /gene="rnhB"
                     /locus_tag="PLS34_06050"
     CDS             complement(6084..6689)
                     /gene="rnhB"
                     /locus_tag="PLS34_06050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRP87065.1"
                     /translation="MSALPLFAAQRIAGVDEAGRGPLAGPVVAAAVILRPGCEIPGLA
                     DSKQLSAAARERLAGQIMAQAGAWAIAWSDAAEIDALNILAASMLAMRRAVDRLGVAP
                     DHVQVDGNRCPRLGRGCTVEALVGGDACVPAISAASILAKTWRDAWMARLEQRYPGYG
                     FAQHKGYPTAAHRAALARLGPCAAHRRSFGPVAVLAAGSGG"
     gene            complement(6686..7897)
                     /gene="lpxB"
                     /locus_tag="PLS34_06055"
     CDS             complement(6686..7897)
                     /gene="lpxB"
                     /locus_tag="PLS34_06055"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003143249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRP87066.1"
                     /translation="MPGWSAQSPTRPRGGLRVGLIAGEPSGDLLGAGLVREIRRCYPD
                     AEFCGIAGPAMLAQGVEGWAPMEQLSVMGVFEVLKDLPALLKIRRQVTGRFMALRPDV
                     VVGIDSPDFTLGVERRLRAAGLRTVHYVSPSIWAWRPGRVHGIGKAADKVLCLLPFEP
                     ALYEAHGIAAEFVGHPMADQVPMAPDRQAARAALGIAAGGSEREVIALLPGSRMGEVE
                     RLGGEFAAAAALLAARHPGVRFVAAMASAPIRARFAAQLAQRAPGLQVQLVDGRAREV
                     MTAADLVLVASGTATLEAALLKRPMVVAYRISALTYRLVAALRLMQTERFALPNLLAG
                     EMLVAELIQDAVTPERIAAEVGALLGDTARRTALADRFAALHETLRQQADRKAAAAVL
                     ELVGRLPEPLA"
     gene            complement(7902..8672)
                     /gene="lpxA"
                     /locus_tag="PLS34_06060"
     CDS             complement(7902..8672)
                     /gene="lpxA"
                     /locus_tag="PLS34_06060"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRP87067.1"
                     /translation="MIHPTAIVASDARIHESVEIGPYSVIGAGVEIGAGSVIGPHVVV
                     NGPTVIGAENRIFQFASIGDAPQDKKYAGEPTRLVIGDRNTIRECVTINRGTVQDTGV
                     TRIGNDNWIMAYAHIAHDCVVGNQVILANNVSLAGHCHVGDWAIFGGFSGLHQFCRAG
                     AHCFMGKYAGVSQDVPAYVMVGGTPAAPHGINAEGLKRRGFSAEQIAHIRHAYKLLYR
                     SGMKLAEALAEIERLAPEQPELEIFAASIRDATRGIVR"
     gene            complement(8669..9127)
                     /gene="fabZ"
                     /locus_tag="PLS34_06065"
                     /pseudo
     CDS             complement(8669..9127)
                     /gene="fabZ"
                     /locus_tag="PLS34_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
     gene            complement(9120..>9597)
                     /locus_tag="PLS34_06070"
     CDS             complement(9120..>9597)
                     /locus_tag="PLS34_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016976865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRP87068.1"
                     /translation="VVIGDDEEIGANTTIDRGAIDDTVIEDGVKLDNQIQVAHNVRIG
                     AHTVVAGCAGISGSTTIGRNCMIAGAVGIAGHLEIADGTVITGLTMVSRSISKPGVYS
                     GALPLDEAASWRRNAARFRQLDQLAKRLARVERRTGSRDDSPGAPADQGDGEAEND"
BASE COUNT         1397 a         3521 c         3300 g         1379 t
ORIGIN      
        1 catccagcag ccctgcggcc tcggcgcaat gccgcgccgc ccgcgacacc gccgcggccg
       61 cgcccggcca gtgcgcgcgc accgccggca gcacctcgtg cctgaggtgg ttgcgcgaca
      121 gctgcggcgc gcggttggcc ggatcctcga gccagcccgc cagtccctcg gcccgcgccc
      181 aggcctccag cgaggcccgc gtgaagccca gcagcggccg ctggtggcgg cccacgccaa
      241 agaccgtgtc cgccggcatg gcggcgagcc ccgccggacc ggccccacgc agcagctgga
      301 tgagcaccgt ctcgagctgg tcgtcggcgt gctgcgccgt cagcagcacc tcccccggcg
      361 cgagccgcgc cgccagcgcc tggtagcgca gctcccgcgc ccgcgcctcc gggctctcgc
      421 cgggcgcggc gcgcgcatcc accgccacca cttccagcgg cacgcccagc tcacggcagc
      481 gcacctcgca gtccgcggcc cagcgattcg catcggcatg cagtccatgg tgcacatgga
      541 tcgcccgcag cccgccgagc gccgctcgcg cgcccagccg cgccatcgcc accagcagca
      601 ccgtcgagtc cagcccgccg ctgaacgcca cgcaatagcg ccgcgacgcg gcgccgccgg
      661 ccccgcccgc agcggcctcg agccgttgcc gcagctcctc gacgctgaac gaattcatgc
      721 cgccgatgtt aacccggcgg ctgctccacg agctcgaagc tctggtaatg gtcgagggcc
      781 tcgcgcagcg tccagcccat gggaccggtg tggacgatgc tcaatgccag ccccgcgatg
      841 gccgtggccc ccggtcgcac gggattatgc atgctgccgg ccctgtcgtt cggcacagcg
      901 cgaccgtaat ctaccgcgag aaggtattca gccgaccgcg ggatgttgag gagcagaaaa
      961 gcgccctccc tgtcgcgctg catatccagg ttgacgcgcg attccgatcg gcggatagcc
     1021 tcgacgatct cgagcaacgg cgcgtcatcc gccgccgcaa acatgatgac cgcatccgcc
     1081 tcgccgttct cggcgtcgcc ggggccctgg tagcggtcca ggaccagcac gagattgcca
     1141 tgatcgttga tccccgcgag caccgcccca tgcagcggcc cgtcgaatgc gagcgccgcc
     1201 gacatcgaca gccccgcgac tccggcaaca gccaccacgc gaaccagcat tgaaatcagg
     1261 ggtttcacga ttcacctcct gcaacgcacg tcgccatata ttcctaccac agcgccaacc
     1321 agcgcctcaa ccgccctcac ggagatcgcc gccgcgcgag ctccgaaccc gggccaccac
     1381 gcagcggcgc cggacgcggt tgtgcgggcc gcgcggcgct cgcgcacagg acgtgcgcga
     1441 gcgagcctac agggatgtat tcacggcgtc cgcgcgggcc gtgcggccgt gaccggcgcc
     1501 gcggtccggc tatgaagcat tcaggctcgg tggaccgcct caaccttccc gaaagcgccc
     1561 cgtgctcacc agcttctggt agcgcgtcgt cagcaactgg tccatcggca gcttgtcgag
     1621 ccgccccagg taacgcgcga tcgccccccg cagcgtctcg gccatggcat cgaaatcgcg
     1681 gtgtgcgccg ccgagcggct cgggcaccac ctcgtcgacc agtccctgct cgaacagctt
     1741 ctcggccgtg atccccatcg cctcggccgc gtcggccgac ttgtcggcgc tcttccacag
     1801 gatcgaggcg cagccctcgg gcgagatcac cgagtagatg ctgtactgga gcatgaggat
     1861 gtgatcggcg acgccgatcg ccagcgcgcc gcccgagccg ccctcgccga tcaccacgcc
     1921 gatgatcggc gtcctgaggc cggccatctc cagcaggttg cgcgcgatgg cctcactctg
     1981 gccgcgttcc tccgcgccga tgcccggata ggcgccgggc gtgtcgatga aggtgatcac
     2041 cggcaggccg aacttctccg ccagcttcat caggcgcaac gccttgcgat agccctcggg
     2101 gcgcggcata ccgaagttgc gctgcacctt ggccttggtg tcgcggccct tctgctggcc
     2161 gacgatcatc accggccggc cctcgaagcg cgccaggccg ccgacgatgg cggcgtcgtc
     2221 ggcgtaggtc cggtcgccgt gcagctcctc gaagtcagtg aacagccgct cgacgtaatc
     2281 gagcgtgtag gggcgctgcg gatggcgggc cacctgggcc acctgccagg ccgtcaggct
     2341 ggagaagatg ttgcgcgtca gcgcctcgct cttcgatttc agccggcgga tctcgctgga
     2401 gagattgacc tgcgagtcgt caccgacgaa gcgcagctcg tcgatcttcg cctcgagctc
     2461 ggcgatcggc tgttcgaaat cgagaaaatt caggttcatg catgtagtcg ggcggccgga
     2521 caggtggcca aaagtaccac agggcgaccg gccgaagaaa acggggtcag accccagttt
     2581 cagtccatca tgcgctccgg cacggcggct ggaaactcgt ctctgacccc gtattccatg
     2641 cgcacgttgt cggcgccgac cagcgcggcg agccggtgca gcagctcgag gctgggccgg
     2701 atccgccagg cctcgcccag ctgcaggcgc gcggcggcgg acgggccgcg gtacaccagg
     2761 gtcaccgggc agcgcccgcc gcagaacggc tgcagcacgt gctgcagctc ggcggcgcag
     2821 cagcaggcgc cggccgcgtt cctgcatggc ggcgtccagg tccacgatgc gcttggcggt
     2881 gaggcgccag tcgtcgatga aatcatcgaa gcgcaggttg ccctcggcga ttacgatcgc
     2941 gtccttggcg accacgtgcc ggcactgctg gtaagtctcc tcgaacagtg tcacctcgag
     3001 ccgcccgctg cggtcgtcga gcaccagcgt gatgcggttg ccgcgccggc gcacgtcgac
     3061 gatcagcccg ccgaccaccg cgtcgcgccg gcccaggccg tagccgcgtt cgccctccgc
     3121 cggccggtcg gcggccagct cggcgatgcg gccgctgctg agcgccgcca ggtccgccgc
     3181 cacctcgtcc accgggtggc cggtcaggaa caggcccagc gtgtcgcgct cgccggcgag
     3241 ccgctccgcc gagctccact cgggcagcgc ggccccctcg tcccccattt cggctgccgc
     3301 gaccgggccg ggtgcggccg ccatgccgaa caggtcctcc tggccggccg ccgcggcgcg
     3361 cgcgctctgc tcggcgcgcc ccagcgcgcc cggcaggcgc tgcatcagcg tggcgcgatt
     3421 ggggccgatg ctgtcgagcg caccggcgcg gatcagcgcc tcgatggtgc ggcggttgac
     3481 ccgctgcagg tcgatgcgcg cgcagaagcc ggtcagcgac tggaagcgcc cgcccgcctc
     3541 gcgctcgttg atgatcgcct gcactgccga ctggccgacg cccttgatgg cgccgagccc
     3601 gtagcgcagc cgtccccggc cggcgacgtt gaaggcgtgc tcggactcgt tcacgtcggg
     3661 cggctcgacc gccagcgcca tctgctggca ctcgtcgatc agcgtgacga tcttgtcggt
     3721 gtggtccatg tcggtggaca gcaccgaggc catgaaggcc tccgggtagt gcgccttgag
     3781 ccacgccgtc tgataggaca gcatggcgta ggccgccgag tggctcttgt tgaagccgta
     3841 gccggcgaac ttctccatca ggtcgaagat gtgcatcgcc gtgccgcgat cgatgtcgcg
     3901 ggccaccgcg ccctcgacga agatggagcg ctgcttggcc atttcctccg gtttcttctt
     3961 gcccatcgcg cggcgcagca ggtcggcggc gccgaggctg tagcccgcca gcacctgcgc
     4021 aatctgcatg acctgctcct ggtacaggat cacgccatgg gtgggtttca gcaccggcgc
     4081 gaggctcggg tggaagtact cgatcgggcc gtcgtggcgg ccgtgcttgc ggttgatgta
     4141 gtcgtcgacc atgccggact gcagcgggcc ggggcggaac agcgccacca gcgcgacgat
     4201 gtcgtcgaag ctgtccggct gcagccgctt caccaggtcc ttcatgccgc gggattccag
     4261 ctggaacacg gccgtggtgc ggcaggccct gagcagctcg aaggtgcggg cgtcgtccag
     4321 cggcaaggcg gccatgtcca gcggcggctc gccggcctcg gcgcgcaaca ggttcacgcg
     4381 cttcaccgcg cggtcgatga tggtcagggt gcgcagcccg aggaagtcga acttcaccag
     4441 cccggccgcc tcgacgtcgt ccttgtcgaa ctgggtgacc ggcgcgccgc cgccctcttc
     4501 gcagtacagc ggcgtgaagt cggtcagcac ggtgggcgcg atcaccacgc cgccggcgtg
     4561 ccggccggca ttgcgcgcca ggccctccag cgacctggcg agctcaatga ggccgcgcac
     4621 ctcgtcctcg gactcgtaca ggcgcttcag ctccggctcc tgggcgagcg ccttgtcgag
     4681 cgtcatgccg acctcgaaag gaatcagctt ggcgatgcgg tcggtgaagc cgtagggatg
     4741 gccgagcacg cggccgacgt cgcgtaccac cgccttcgcc gccatgctgc cgtaggtgat
     4801 gatctgcgcc acgctgtcgc gaccgtagcg ctgcgccacg taatcgatca cgcggtcgcg
     4861 gccgttgatg cagaaatcga tgtcgaagtc cggcatcgag acccgctccg ggttgagaaa
     4921 gcgctcgaag agaaggtcgt aacgtaatgg atctaaatca gttattccaa gcacgaaggc
     4981 gaccagcgag ccggcgcccg agccgcggcc cggccccacc ggaatgtcgt tgtccttcgc
     5041 ccagcggatg aagtcggcga cgatcaggaa gtaacccggg aagcccatgt cgcaaatcac
     5101 gtcgagctcg cgcgccagcc ggctgcggta gtcgtcggcc gagtacgccg gcgcggccgg
     5161 cagcagggcc gggaggcgcg cctcgagggc ggcgccggcc agcgcccgca ggtgactgcc
     5221 ggcggtctcg cccgccggca ccgggtagtc gggcaggaag gtctggccga gctccagctc
     5281 gacgctgcag cgacgcgcga tttccacgct gttctccagc gcctcgggca ggtcggagaa
     5341 cagcgccgcc atctccgcgg cgctcttgag atactgctcc tcgctgtagt cgcggggccg
     5401 gcgcggatcg ctgagggtgt gcccctggtg gatgcacacg cgcgcctcgt gcgcgtcgaa
     5461 gtcggccgca tcgaggaagc gcacgtcgtt gctcgccacc accggcgtgc cgctcgccgc
     5521 cgcgagcgcc accgcctcgg cgacgtggaa ctcgtcgccc tcgcgcccgg tgcgctgcag
     5581 ctccaggtag aagctggccg ggaagcgcgc gcgccagaag gccaggcagt ccgccgccgt
     5641 gtcgcgccgc ccggccacca gcgcgcggcc gatgtcgccc tcgcgcgccg ccgacagggc
     5701 gatcagcccg ctgcaggcgc tcggcgtcag ccagtcgcgc tcgatgagca cctggcccag
     5761 gcggcgccct tccaggtagg cgcgggtcag cagccggcac aggttgcgat agccctcggc
     5821 gttgcggcac aacagtgcga ggcggctggg atggcgcgga tcgccgggct cctcgatcca
     5881 gatgtcggcg ccgatcagcg gcttgatgcc gtgcttgagc gcgccacgat agaacttgac
     5941 catcgagaac aggttcacct ggtcggtcac cgcgaccgcc ggcatgccga gctttccgac
     6001 ccgctcgagc agcgccggaa tacggatcac gctgtccgcc agcgagtact cggtgtgcac
     6061 tcgcaggtgg acgaagccgg cgctcacccg ccgctcccgg ccgccaggac ggccaccggc
     6121 ccgaaggaac gccggtgcgc cgcgcagggc cccagccgcg ccagcgccgc gcggtgcgcc
     6181 gccgtcgggt agcccttgtg ctgcgcaaag ccgtagcccg ggtagcgctg ctccagccgc
     6241 gccatccagg cgtcgcgcca ggtcttggcc aggatcgatg cggcgctgat cgccggcacg
     6301 caggcgtcgc cgcccaccag cgcctcgacc gtgcagccgc ggcccagccg cgggcagcgg
     6361 ttcccgtcca cctgcacgtg gtcgggcgcc acgccgagcc ggtccacggc acggcgcatg
     6421 gcgagcatgc tggccgcgag gatgttgagc gcgtcgatct cggccgcgtc cgaccaggca
     6481 atcgcccagg cgcccgcctg cgccatgatc tgtccggcca gccgttcacg ggccgccgcg
     6541 ctgagctgct tggaatcggc cagccccgga atctcgcagc cgggccgcag gatgacggcc
     6601 gcggccacca ccgggccggc cagcggcccc cggcccgcct cgtccacgcc ggcgatgcgc
     6661 tgcgcggcga acagcggcag cgcgctcacg ccagcggctc cggcaggcgc ccgaccagct
     6721 cgagcaccgc cgctgcggcc ttgcggtccg cctgctggcg cagggtctcg tgcagggcgg
     6781 cgaagcggtc agccagcgcc gtgcggcgcg cggtgtcgcc gagcaaggcg cccacctccg
     6841 ccgcaatgcg ctccggagtg acggcgtcct ggatgagttc cgcgaccagc atctcgcccg
     6901 ccagcaggtt cggcagcgcg aagcgctcgg tctgcatcag ccggagcgcc gccaccagcc
     6961 ggtaggtgag cgccgagatg cgataggcca ccaccatcgg ccgcttcagc agcgcggcct
     7021 ccagcgtggc ggtgcccgag gcgaccagca ccaggtcggc cgcggtcatc acctcgcgcg
     7081 cgcggccgtc gaccagctgc acctgcagcc cgggggcgcg ctgcgccagc tgcgcggcga
     7141 agcgcgcgcg gatcggcgcg gaggccattg cggcgacgaa gcgcacgccg ggatggcggg
     7201 ccgccagcag ggcggcggcc gccgcgaact cgccgccgag ccgctcgacc tcgcccatgc
     7261 ggctgccggg cagcagcgcg atcacttcgc gctcgctgcc gcccgcagcg atgccgagcg
     7321 cggcgcgtgc ggcctgccgg tcgggcgcca tgggaacctg gtccgccatg ggatggccga
     7381 cgaactcggc cgcgatgccg tgtgcttcgt acagcgccgg ctcgaagggc agcaggcaca
     7441 gcaccttgtc ggccgccttg ccgatgccgt gcacgcgacc cggccgccag gcccagatgg
     7501 aggggctgac gtaatgcacc gtgcggaggc cggcggcgcg caggcgccgc tcgacgccga
     7561 gcgtgaagtc cggcgagtcg atgccgacca ccacatcggg gcgcagcgcc atgaaacgcc
     7621 cggtcacctg gcgacggatc ttcagcagcg ccggcagatc cttgagcacc tcgaacacgc
     7681 ccatcaccga gagctgctcc atcggcgccc agccctcgac gccctgcgcg agcatcgcgg
     7741 gaccggcgat gccgcagaac tccgcatccg ggtagcagcg gcggatttcg cgcaccagcc
     7801 cggcgccgag caggtcgccg gagggctcgc ccgcgatcag cccgacgcgc aggccgcccc
     7861 gcggccgggt tggggattgg gcgctccagc caggcatcgc cctagcgtac gatgccgcgc
     7921 gtggcgtccc ggatggaggc cgcgaagatc tccagctcgg gctgctccgg cgcgagtctc
     7981 tcgatctcgg cgagcgcctc ggccagcttc atgccgctgc ggtacagcag cttgtaggcg
     8041 tggcggatgt gcgcaatctg ctcggcgctg aagccgcggc gcttgagccc ctcggcgttg
     8101 atgccgtgcg gtgcggccgg cgtgccgccg accatcacgt aggccggcac gtcctggctc
     8161 acgcccgcgt acttgcccat gaagcagtgc gcgcccgcgc ggcagaactg gtgcagcccc
     8221 gagaagccgc cgaagatcgc ccagtcgccg acgtggcagt ggccggcgag cgagacgttg
     8281 ttggcgagga tgacctggtt gcccaccacg cagtcgtggg cgatgtgcgc gtaggccatg
     8341 atccagttgt cgttgccgat gcgcgtcacg cccgtgtcct gcacggtgcc gcggttgatg
     8401 gtgacgcact cgcggatggt gttgcggtcg ccgatcacca gccgcgtcgg ctcgccggcg
     8461 tacttcttgt cctgcggggc gtcgccgatg gaagcgaact ggaagatgcg gttctctgcg
     8521 ccgatcacgg tcgggccgtt gacgaccacg tgcggcccga tcaccgagcc ggcgccgatt
     8581 tccacgcccg ccccgatgac cgaataagga ccgatttcca cgctctcgtg gatgcgcgca
     8641 tcggaggcca cgatcgccgt cgggtgaatc acgatcggcc tccctccggt gcaaccatca
     8701 tctcggcaga ggcgaccacc ttgccgtcca cgctcgcctc ggtctggaag cgccagatcg
     8761 tgcggatgcg gcgctccacc cgggccttga gcagcagctg gtcgcccggc acgaccgggg
     8821 ccctgaagcg cgccttgtcg atgcccacga aatagaaggt cgaattctcg gtcggctcga
     8881 cgccggcggt gcgaaaggcc agcacgcccg cggcctgcgc catggcctcg atgatcagca
     8941 cgcccggcat gatcgggcgg ccgggaaagt gtccctggaa gaagggctag ttgcaggtga
     9001 cgtacttgat ggcggtgatc tcgccgcccg gcgtgtgctc cagcacccgg tcgatgagca
     9061 ggaagggata gcggtgcggc aggtacacca tgatccgttg caggtcgagt gagctgtcgt
     9121 cagtcattct ccgcctctcc gtcgccttgg tccgccggcg cccccggcga atcgtccctg
     9181 gaacccgtgc ggcgctcgac gcgcgccagg cgcttcgcca gctggtccag ctggcggaag
     9241 cgcgccgcgt tgcgccgcca gctcgccgcc tcgtcgagcg gcagcgcgcc cgagtatacg
     9301 ccgggcttgc tgatcgagcg gctgaccatg gtcaggcccg tgatgaccgt gccgtcggcg
     9361 atctcgagat ggccggcgat gcccacggcc cccgcgatca tgcagttgcg gccgatggtg
     9421 gtgctgcccg agatgccggc gcaacccgcc accacggtat gcgccccgat acgcacgttg
     9481 tgggccacct ggatctggtt gtccagcttg acgccgtcct cgatcaccgt gtcgtcgatg
     9541 gcgccgcggt cgatggtggt gttcgcgccg atttcctcgt cgtcgccgat cacgacc
//
LOCUS       DAOMPG010000082         9417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3142_length_9417_cov_3.273232, whole genome shotgun sequence.
ACCESSION   DAOMPG010000082 DAOMPG010000000
VERSION     DAOMPG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_9417_cov_3.273232"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="queG"
                     /locus_tag="PLS34_06075"
     CDS             complement(<1..752)
                     /gene="queG"
                     /locus_tag="PLS34_06075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRP87069.1"
                     /translation="MKLDPTPATADATLPTLAGLARDVRTWGRELGFGALGIARAELP
                     EDEAWLKRWLALGRHGEMAWMARHGTRRSRPAELVPGTVTVIAVRMDYLPTEGADPEQ
                     VLADPRLGYVSRYALGRDYHKVLRQRLQRLAERIGDAAGPFGYRVFTDSAPVLEKALA
                     RDAGLGWIGKHTNLLARDAGSWFFLGEIYTDLPLPTDTADSAHCGSCTACIEACPTGA
                     IVAPYELDARLCISYLTIEHAGSIPAPLRPAFG"
     gene            792..>1193
                     /locus_tag="PLS34_06080"
     CDS             792..>1193
                     /locus_tag="PLS34_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRP87070.1"
                     /translation="MQATPRELWSAAQVRELDRRAIEEHGIPGRELMERAGAFALDAL
                     RQRWPATRALTVVCGAGNNGGDGYVVARLARRAGLNVRALALADPAALRGDAAAACAD
                     FRGDGGEILAWDGAGDPGGDGPLVDALLGTGL"
     assembly_gap    1194..1258
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1259..2308
                     /locus_tag="PLS34_06085"
     CDS             <1259..2308
                     /locus_tag="PLS34_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRP87071.1"
                     /translation="AGRPVLALDVPSGLHADTGRVMGAAVAADLTACFIGLKLGLFTG
                     RGPGLAGDVAFSALDTPRGLDRGLQPVARRLEPALLGDWLPRRPRDAHKGRFGHVLVI
                     GGDDGMGGAVRLAAEAALRAGAGLVSVATRPAHVAPILAARPELMCHGVQGPDALESL
                     LARATVVAVGPGLGRAAWGQALLGAALESGLPCVLDADALNLLSEATRRSSRWILTPH
                     PGEAGRLLGSDAATIEADRPAAVHELRARYGGVVVLKGAGTLVGGAGGPLWLCDRGNP
                     GMASGGMGDVLTGVVAGLAAQLGDLERSACAGVLVHALAGDDAARRGERGLAAADLLD
                     ALRPWVNPVDARPAG"
     gene            2289..2801
                     /gene="tsaE"
                     /locus_tag="PLS34_06090"
     CDS             2289..2801
                     /gene="tsaE"
                     /locus_tag="PLS34_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRP87072.1"
                     /translation="MPVRLADPAATEALGARLAGPLAAASGAAPVVVHLAGPLGAGKT
                     TLARGLLRGLGHAGRVRSPTFTLLEPYELPACQVIHLDLYRLADPGELEYLGLVDMLV
                     PGTIALVEWAERGGDRLPPADLRITLDYEGAGRCALLAGLTPAGSALLAAAGSPGEPP
                     GVHPEAGSGG"
     gene            2911..4224
                     /locus_tag="PLS34_06095"
     CDS             2911..4224
                     /locus_tag="PLS34_06095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRP87073.1"
                     /translation="MQRIILTGVLLAALLTVPAAGAEVAVREVRLWEGPDSTRVVLDL
                     SQPAGHRLFTLDNPSRVVVDVKPANIDLGRVRLPEGVGLVQSVRGANRPGGEVRIVLD
                     LAGDAAPRSFLVRPEGRYGHRLIVELEPRRARAQQPVIRADEGSRDLVIAIDAGHGGE
                     DPGAIGRKGTYEKDIVLQIARRLAQEIDREPGMRAVLTRDGDYFLSLQQRVAIARKHR
                     ADLFVSIHADAFKNPNARGSSVYVLSPKGATDEAARWLAARENAADLVGGVSLDDKDD
                     MLASVLLDLSQNASIGASLEVGEFVLNRLSGVNRLHKSSVQQAGFVVLKAPDIPSILV
                     ETAFISNNEEEQRLRDGRYQAQLAQALMAGVREYFYVNPPPGTWVAQNRNRGGGGPRE
                     VVIARGDTLSGIASRYNVSVPVLRRHNGLKNDNIRVGQVLTIPGG"
     gene            4252..6042
                     /gene="mutL"
                     /locus_tag="PLS34_06100"
     CDS             4252..6042
                     /gene="mutL"
                     /locus_tag="PLS34_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRP87074.1"
                     /translation="MPIRQLPPQLIDQIAAGEVVERPASVIKELVENSLDAGATRIEL
                     EIDGGGLRQCVVRDNGCGIPRDELALALSRHATSKIASLEDLERVGTLGFRGEALPSI
                     ASVSRLSVTSRARGAEAAWRVESDGGRLSAPAPAAHPEGTTLVVRDLFYNTPARRRFM
                     RTERTEFGHVERVARRLALSRFDAGFRLLREGKPLFDLRPAADTGTRTGRIAEVCGEA
                     FAAHAMEIDQATAGLRLSGWLGLPTFSRAQPDLQYFFVNGRMIRDRLVTHALRQAYAD
                     VLFHGRHPAYVLYLEMDPALVDVNAHPTKHEVRFRDARSMHGFLYKAVERALAQTRPA
                     PGAAAAAPPPATPRFGSESQGGEWIGPTRQDAMRLAVRDAEALPQHGWPAFTAAAEAA
                     VPDAAAAPPDDPPLGYALGQLGGIYILAQNRDGLVVVDMHAAHERVLYERLKVAMSGP
                     DAAGQPLLVPLSVRVSPSEAELAESVAESLARFGFELDRRGPDVLVVRQVPALLKSAD
                     AETLLRDILSDLAEQGDSSRLEERVNQVLGTMACHGAVRANRQLTVAEMNALLRAMER
                     TERADQCNHGRPTWTRLTLPELDKLFLRGR"
     gene            6051..6995
                     /gene="miaA"
                     /locus_tag="PLS34_06105"
     CDS             6051..6995
                     /gene="miaA"
                     /locus_tag="PLS34_06105"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRP87075.1"
                     /translation="MSSAPAILLMGPTASGKTDLAIALARALGGEIVSVDSALVYRGL
                     DIGTAKPSPALRAEIPHHLVDILDPAEPYSAGRFRRDALVAMAQVRGRGRTPILVGGT
                     MLYFRALLRGLAELPAADPALRAALDAEAAQRGWPALHAELARVDPAAAARIRPGDSQ
                     RIQRALEVWRLTGRPLSELQAAGAALPEGWQFLKLALAPPTRAALHAAIGARFEAMMA
                     AGLLAEVEALYARGDLHAGLPAIRAVGYRQLWAHLAGECELERAIADAVTATRRLAKR
                     QMTWLRAEPGLHWLESAAGGLPAATGWLAGAAGGAGRL"
     gene            7098..7346
                     /gene="hfq"
                     /locus_tag="PLS34_06110"
     CDS             7098..7346
                     /gene="hfq"
                     /locus_tag="PLS34_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013795956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRP87076.1"
                     /translation="MAKGQSLQDPFLNALRREKVPVSIYLVNGIKLQGQIESFDQFVV
                     LLKNSVSQMVYKHAISTVVPSRNVRLGGDDAFDDAAED"
     gene            7368..8696
                     /gene="hflX"
                     /locus_tag="PLS34_06115"
     CDS             7368..8696
                     /gene="hflX"
                     /locus_tag="PLS34_06115"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRP87077.1"
                     /translation="MFDRPGHGERAVLVQIGFGARPSEEELEELERLALSAGATVAGR
                     LTATRAAPDARHFIGKGKVEELAGLIATTGADLVLFNHALSPSQERNLEKALSCRVLD
                     RTGLILDIFAQRARSFEGKLQVELAQLRHLSTRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLANRIRMLTRRLERVDKQREQGRRTRAKAEVPTVALVGYTNAGKSTLFNALT
                     ESRVYAADKLFATLDPTLRGLELAPGLDVVLADTVGFVRDLPHELVAAFRSTLKETRD
                     AALLLHVVDIADPLRDERIGDVNRVLEEIGAERVPQLLVYNKADLGGEPPGLLRDEAG
                     KVRGVRLSARTGAGLELLIDALREHFGEGQVAGTVHLDASEARMRAKLYDADMVRKES
                     EDQAGGWNLELQAPKVALERFCRDTGLNRGRIRPHPCEGLAASLQSRTSA"
     gene            8750..>9417
                     /locus_tag="PLS34_06120"
     CDS             8750..>9417
                     /locus_tag="PLS34_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HRP87078.1"
                     /translation="MAWNEPGKGGNPWNSGGRKDGPPDLDKVVRDLQRKISGMFGGRG
                     GGSGGSGGSGGAVAGAGLGLIGAAAVVLWALSGFYQVDEAERGVVLQFGSFHEITMPG
                     LHWHIPYPIQRVEKVNVAQVDSFTHSTRMLTADENIVTVDLRVQFRRADARAFLFNVR
                     FPEETLGEVTETAIREVVGKSQLDFVLTEGRTEVAARTREIIQATLDSYGAGIEITSV
                     NLEDS"
BASE COUNT         1286 a         3276 c         3435 g         1355 t
ORIGIN      
        1 ccgaaggccg ggcgcagcgg cgcggggatc gaaccggcgt gctcgatggt caggtaggag
       61 atgcacagcc gtgcgtccag ttcgtagggc gcgacgatcg cgccggtggg gcaggcctcg
      121 atgcacgcgg tgcagctgcc gcagtgcgca ctgtccgcgg tgtcggtcgg caacggcagg
      181 tcggtgtaga tctcgccgag aaaaaaccag ctgcccgcgt cgcgcgccag caggttggtg
      241 tgcttgccga tccagccgag cccggcgtcg cgcgccagcg ccttctccag caccggcgcg
      301 ctgtcggtga acacgcggta gccgaagggc cccgccgcgt cgccgatccg ctccgccagc
      361 cgctgcaggc gctggcgcag caccttgtga taatcgcgtc ccagcgcgta gcgcgacacg
      421 tagccgagcc gcggatccgc cagcacctgc tccgggtcgg cgccctcggt cggcaggtaa
      481 tccatgcgca ccgcgatcac ggtgacggtg ccgggcacga gctcggccgg ccggctgcgg
      541 cgcgtgccgt ggcgcgccat ccaggccatc tcgccgtggc gaccgagcgc cagccagcgt
      601 ttcagccacg cctcgtcctc cggtaactcg gcccgggcga tgcccagcgc gccgaagccc
      661 agttcgcggc cccaggtgcg cacgtcgcgc gccagcccgg ccagcgtggg cagggttgcg
      721 tcggcggtgg ccggtgtcgg atcgagcttc atggcgcggg tatctccctg gccggcagta
      781 taatccgcgc catgcaagcc acgccccgcg aactctggag cgccgcgcag gtgcgcgagc
      841 tggaccgccg cgccatcgag gagcacggca tccccggtcg cgagctcatg gagcgcgccg
      901 gcgccttcgc gctggacgcc ttgcggcagc gctggcccgc gacacgcgcg ctcaccgtgg
      961 tctgcggcgc gggcaacaac ggcggcgacg gctacgtcgt ggcgcgcctc gcccggcgcg
     1021 ccggactgaa cgtgcgcgcc cttgccctgg ccgatcccgc cgcgctgcgc ggcgatgcgg
     1081 cggcggcctg cgcggacttc cgcggcgacg gcggcgaaat cctggcgtgg gacggcgcgg
     1141 gcgaccccgg cggcgacggt ccgctggtgg acgcgctgct cggcaccggg ctcnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     1261 gggccggcca gtgctggcgc tggacgtgcc cagcggcctg cacgccgaca cgggccgcgt
     1321 gatgggcgcg gcggtcgcgg cggacctgac ggcttgtttc atcggcctca agctcgggct
     1381 gttcaccggc cgcgggccgg ggctggcggg cgacgtggcc ttctccgcgc tggacacgcc
     1441 gcgcgggctc gatcgcggcc tgcagccggt ggcgcgccgc ctggaaccgg cgctgctcgg
     1501 cgactggctg ccccgccggc cgcgcgatgc gcacaagggc cgcttcggcc acgtgctggt
     1561 gatcggcggc gacgacggca tgggtggcgc cgtccggctg gccgccgagg cggcgctgcg
     1621 cgccggcgcg gggctggtga gcgtcgcgac gcgcccggcg cacgtggcgc cgattctcgc
     1681 ggcgcggccc gaactgatgt gccacggcgt gcagggcccc gacgcgctgg agtccctgct
     1741 ggcgcgcgcc acggtggtcg cggtcgggcc ggggctcggg cgtgccgcct ggggccaggc
     1801 gctgctcggg gcggcgctgg aatccggact gccctgtgtg ctcgacgcgg atgcgctgaa
     1861 cctcttgtcc gaggcaacgc gccgctcgtc gcgctggatc ctgacgccgc atcccggcga
     1921 ggccggccgc ctgctcggca gcgatgcggc gaccatcgag gcggatcgtc cggccgccgt
     1981 gcacgaattg cgcgcgcgct acggcggcgt ggtggtgctc aagggcgcgg gcacgctggt
     2041 gggcggcgcc ggtggcccgc tctggctgtg cgatcgcggc aatcccggca tggcctccgg
     2101 cggcatgggc gacgtgctca ccggcgtggt cgccgggctg gcggcgcagc tcggcgacct
     2161 ggagcgttcg gcgtgcgccg gcgtgctggt gcatgcgctt gccggcgacg acgcggcccg
     2221 acgcggcgag cgcggactgg cggcggccga cctgctcgac gccctgcgtc cctgggtaaa
     2281 tcccgtcgat gcccgtccgg ctggctgatc ccgcggcgac cgaggcgctc ggcgcccggc
     2341 tcgccgggcc gctggcggcg gccagcggtg ccgcgccggt cgtggtccac ctcgcgggcc
     2401 cgctcggcgc cggcaagacc acgctggcgc gcggtctgct gcgcgggctg ggccacgcgg
     2461 ggcgggtgcg cagcccgacc ttcacgctgc tcgagcccta cgagttgccg gcttgccagg
     2521 tgatccacct cgacctctac cggctggccg accccggcga gctggagtac ctcgggctgg
     2581 tcgacatgct cgtacccggc accatcgcgc tggtggagtg ggccgagcgg ggcggcgacc
     2641 gcctgccgcc ggcggacctg cgcatcacgc tggactacga gggcgcgggc cgctgcgccc
     2701 tgctggcggg tctgacgccc gccggcagcg ccctgcttgc ggcggcaggc tcacctggtg
     2761 agccacccgg ggtccatccg gaggcgggca gcggcggata agaaaagtcc ggaactttca
     2821 gctgcagccg cttgtttctg cggcagatat tgccaccagc cccggaatgt ggttaaagtt
     2881 gcttcccgat atccaaccaa acagttggaa atgcaacgaa ttatcctcac cggcgtgctc
     2941 ctggctgcgc tcctgacggt cccggccgcc ggggccgagg tggcggtgcg cgaagttcgc
     3001 ctgtgggagg gcccggattc gacccgcgtc gtgctcgacc tgtcgcaacc ggcgggccac
     3061 cgcttgttca ccctcgacaa tcccagccgg gtcgtagtcg acgtcaagcc ggcgaacatc
     3121 gatctcggcc gcgtgcgcct gccggaggga gtcggcctgg tgcagtcggt gcgcggcgcc
     3181 aatcgccccg gcggcgaagt gcgcattgtg ctggacctgg ccggggacgc tgcgccgcgc
     3241 agcttcctgg tgcggcccga gggccgctac gggcaccgcc tgatcgtcga gctcgagccc
     3301 cggcgagccc gggctcaaca gccggtgatc cgggccgacg aaggcagccg cgacctggtg
     3361 atcgccatcg atgcgggtca cggcggcgag gacccgggtg ccatcggccg caaggggact
     3421 tacgagaagg acatcgtcct gcagatcgcc cggcgacttg cgcaggagat cgaccgcgag
     3481 cccggtatgc gtgccgtgct gacgcgcgac ggcgactact ttctcagcct gcagcagcgc
     3541 gtggcgatcg ctcgcaagca ccgggccgac ctgttcgtat cgattcacgc cgacgcgttc
     3601 aagaacccga acgcgcgcgg ctcctccgtg tacgtgctgt cgccgaaggg cgccaccgac
     3661 gaagcggccc gctggctggc ggcacgcgag aacgccgccg acctggtggg cggtgtgagc
     3721 ctggacgaca aggacgacat gctcgcctcg gtactactcg acctgtcgca gaacgcttcg
     3781 atcggcgcca gcctggaggt cggtgagttc gtgctgaacc ggctcagcgg cgtgaaccgc
     3841 ttgcacaaga gctcggtcca gcaggccggc ttcgtggtgc tgaaggcgcc ggacatcccc
     3901 tccatcctgg tcgagacggc cttcatctcg aacaacgagg aagagcagcg gttgcgcgat
     3961 ggtcgctacc aggcgcagct ggcgcaggcg ctcatggccg gggtgcgcga gtacttctac
     4021 gtcaacccgc cacccggcac ctgggtggcc cagaaccgca atcgcggcgg cggcgggccg
     4081 cgcgaagtcg tgatcgcgcg cggcgacacg ctctcgggca tcgccagccg ttacaacgtc
     4141 agcgtcccgg tcctgcgccg ccacaacggc ctcaagaacg acaacatccg cgtcggccag
     4201 gtattgacca tccccggcgg ctgacggcgg tttggtatcc ttgcgatacc gatgcccatc
     4261 cgccagctcc ccccgcagct catcgaccag atcgccgccg gcgaggtggt cgagcgcccg
     4321 gcgtccgtca tcaaggaact cgtcgagaac agcctcgacg caggcgccac acggatcgaa
     4381 ctggagatcg acggcggcgg gctgcgacag tgcgtggtac gcgacaacgg ctgcggcatt
     4441 ccgcgcgacg agctggcgct ggccctgtcg cgccatgcga ccagcaagat cgccagcctc
     4501 gaggacctcg agcgggtcgg cacgctgggc ttccgcggtg aggccctgcc gagcatcgcc
     4561 tcggtctcgc gcctgtcggt cacctcgcgg gcgcgcggcg ccgaagcggc ctggcgggtc
     4621 gagtccgacg gcgggcggtt gagcgcgccg gcgcccgcgg cgcatcccga gggcaccacc
     4681 ctcgtcgtgc gcgacctgtt ctacaacacg ccggcgcgac gtcgcttcat gcgcacggag
     4741 cgcaccgagt tcgggcacgt tgaacgggtc gcccgccggc tcgcgctgtc gcgtttcgat
     4801 gcgggctttc gcctgctgcg cgagggcaag ccattgttcg acctgcggcc ggctgccgac
     4861 accggcacgc gcaccggccg catcgccgag gtctgcggcg aagccttcgc ggcgcacgcc
     4921 atggagatcg accaggccac cgccggcctg cggctgtccg gctggctcgg cctgccgacc
     4981 ttttcccggg cgcaacccga cctgcagtat ttcttcgtca acggacgcat gatccgcgac
     5041 cggctggtga cccacgcgct gcgccaggcc tatgcggacg tgctgtttca tggccgtcac
     5101 ccggcctatg tcctttatct cgagatggat ccggcgctgg tggacgtcaa cgcgcatccg
     5161 accaagcacg aagtgcgctt tcgcgacgcc cgcagcatgc acggcttcct ctacaaggcg
     5221 gtggagcgcg cgctcgccca gacccgcccg gctcccggcg ccgccgcggc cgcaccgccg
     5281 ccggccacgc cgcgcttcgg ctccgagtcg cagggtgggg aatggatcgg gccgacgcgc
     5341 caggacgcca tgcgactcgc ggtgcgggac gcggaggcat tgccgcagca cggttggccg
     5401 gcgttcaccg ctgcggcgga ggcggccgtg ccggacgccg cggcggcccc gccggatgac
     5461 ccgccgctgg gctacgccct cgggcagctc ggcggcatct acatcctggc gcagaaccgc
     5521 gacgggctgg tggtcgtcga tatgcatgcg gcgcatgagc gcgtgctcta cgagcgcctc
     5581 aaggtggcga tgtccggtcc ggacgccgcc ggccagccgt tgctggtgcc gctcagcgtg
     5641 cgggtctcgc cgagcgaggc cgagctggcg gaatccgtgg cggaatcgct cgcccggttc
     5701 ggcttcgagc tcgaccggcg cggccccgac gtgctggtgg tgcgccaggt gccggccctg
     5761 ctgaagtccg cggacgccga gacgctgctg cgcgacatcc tctccgacct ggccgagcag
     5821 ggcgacagca gccggctgga ggagcgggtc aaccaggtgc tcggcaccat ggcctgtcac
     5881 ggcgcggtgc gcgccaaccg gcagctcacg gtcgcggaaa tgaacgccct gctgcgcgcc
     5941 atggaacgca cggagcgcgc cgaccagtgc aaccacggcc gcccgacctg gacccggctg
     6001 accctccccg agctggacaa gctcttcctg cgcggccgct gaagccgtcc atgagcagcg
     6061 cgccggcgat cctgctgatg ggcccgacgg cctccggcaa gacggatctc gccatcgcgc
     6121 tggcacgcgc gctgggcggc gagatcgtca gcgtcgactc ggcgctggtc taccgcggcc
     6181 tcgacatcgg cacggccaag ccctcgccag cgctgcgcgc ggagattccg caccacctgg
     6241 tagacatcct cgatccggcg gagccttatt ccgccggccg gttccgtcgc gatgcgctcg
     6301 tcgcgatggc gcaggtgcgt ggccgcggtc gtacgccgat cctggtcggc ggcaccatgc
     6361 tgtactttcg ggcgttgctc agggggctgg cggagctgcc agcagccgat ccggcgctgc
     6421 gcgcggcgct ggatgcggag gcggcgcagc gcggttggcc ggcgttgcac gcggagctgg
     6481 cccgcgtgga cccggcggcg gccgcccgga tcaggcccgg ggactcacag cgcatccagc
     6541 gcgcgctcga ggtctggcgg ctgaccggca ggccgctgtc cgagctgcag gcagccggcg
     6601 cggcgctgcc ggagggctgg cagtttctca agctggcgct ggcgccgccc acgcgcgcgg
     6661 cgctgcacgc tgccatcggc gcccgtttcg aggcgatgat ggcggccggg ctgctcgccg
     6721 aggtcgaggc gctgtacgcc cgcggcgacc tgcacgccgg gttgccggcc atccgcgcgg
     6781 tgggttaccg gcagctgtgg gcgcacctgg cgggcgagtg tgagctcgaa cgcgcgatcg
     6841 ccgacgcggt caccgccacc cggcgcctcg ccaagcggca gatgacctgg ctgcgcgccg
     6901 agcccggcct gcactggctc gaaagtgccg ccggcgggct gccggcggcg actggctggc
     6961 tcgccggcgc agccggtggg gcggggaggt tgtgataaac ttcgccttcg ggcgtcgggg
     7021 cgcgggccgg ctcttgcctg accggtggag ggaactcccg gatcaataat atcggcccga
     7081 gtgggagagt tcagccaatg gccaaagggc agtcgctgca ggacccgttc ctcaatgcct
     7141 tgcgcaggga gaaggtgccg gtgtccatct atctcgtgaa cggcatcaag ctacagggcc
     7201 agatcgaatc cttcgaccag ttcgtcgtgc tgctcaagaa cagcgtgagc cagatggttt
     7261 acaagcacgc gatctccacc gtggtgcctt cgcgcaacgt ccgcctcggc ggcgacgacg
     7321 cgttcgacga cgcggcggaa gactgagccg ggcgccggga ggatttcttg ttcgatcgtc
     7381 ccgggcacgg tgagcgtgcc gttctcgtcc agatagggtt cggcgccagg ccttccgaag
     7441 aggaactcga ggagctcgag cggctggcct tgtccgcggg cgcgaccgtc gccggccgcc
     7501 tgaccgccac gcgcgcggcg cccgacgcac ggcatttcat cggcaagggc aaggtcgagg
     7561 agctggccgg tctgatcgcc accacgggcg ccgatctcgt gctgttcaac catgccctct
     7621 cgcccagcca ggagaggaat ctcgagaagg cgctgagctg ccgggtgctg gatcgcaccg
     7681 gcctgatcct cgatatcttt gcgcagcgcg cgcgcagttt cgagggcaag ctgcaggtcg
     7741 agctggccca gctcaggcat ctctcgaccc ggctggtgcg cggctggact cacctcgagc
     7801 ggcagaaggg cggcatcggc ctgcgtggtc cgggcgaaac ccagctggaa accgaccgcc
     7861 ggctgctcgc caaccgcatc cgcatgctga cgcgcaggct cgagcgggtg gacaagcagc
     7921 gcgagcaggg ccgacgcacg cgcgccaagg ccgaagtgcc cacggtcgcg ctggtcggtt
     7981 acaccaacgc cggcaaatcg acgctgttca atgcgctgac cgagtcgcgc gtctatgcgg
     8041 ccgacaagct gttcgcgacg ctcgatccga cgctgcgggg cctggagctt gcgccggggc
     8101 tggacgtggt gctggccgac accgtcggct tcgtgcgcga cctgccgcac gaactggtgg
     8161 cggccttccg gtccaccctg aaggaaaccc gcgatgcggc attgctgctg cacgtggtcg
     8221 acatcgccga tccgctgcgc gacgagcgca tcggcgacgt caaccgcgta ctcgaggaga
     8281 tcggcgcgga acgcgtgccg cagttgctgg tctataacaa ggcggatctc ggcggcgaac
     8341 cgcccgggct gctccgtgac gaggcgggca aggtgcgggg ggtgcggctg tcggcgcgca
     8401 ccggggccgg gctggagctg ctcatcgacg cactacgcga gcatttcggc gaagggcagg
     8461 tcgcgggcac cgtgcatctc gatgcatcgg aggcgcgcat gcgggcgaaa ctgtacgatg
     8521 ccgacatggt gcgcaaggag tcggaggacc aggcgggcgg ctggaacctg gagttgcagg
     8581 ccccgaaggt ggcgctggag cgattctgtc gcgatactgg cctgaatcgg ggtcgcatcc
     8641 ggccgcatcc ttgtgagggc ctggcggcgt ccctacaatc ccgcacgtcg gcgtgagcgc
     8701 gaagccgcgc acgggcgtcg cgaagcgaag cgaaatctga aggataatca tggcttggaa
     8761 tgaacccggc aagggcggca acccctggaa ctccggcggc cgcaaggacg gcccgcccga
     8821 cctcgacaag gtcgtgcgcg acctgcaacg caagatctcc gggatgttcg gcggccgtgg
     8881 cggcggttcg ggcggttcgg gcggctcggg tggcgcggtg gcgggcgcgg gactcggcct
     8941 gatcggggcg gcggccgtgg tgctgtgggc cttgtcgggc ttctaccagg tggacgaggc
     9001 ggaacggggc gtggtgctcc agttcggctc cttccacgag atcaccatgc ccggcctgca
     9061 ttggcacatt ccctatccca tccagcgggt cgaaaaggtc aacgtcgcgc aggtcgactc
     9121 tttcacgcac tccacgcgca tgctgaccgc ggacgagaat atcgtcacgg tggatttgcg
     9181 cgtgcagttc cgccgtgcgg acgcccgcgc tttcctgttc aacgtgcgct tcccggagga
     9241 gacgctcggc gaggtgaccg agacggccat ccgtgaagtg gtcggcaaga gccagctcga
     9301 cttcgtgctg accgagggcc gtaccgaagt ggcggcgcgc acgcgcgaga tcatccaggc
     9361 caccctcgac tcctacggcg ccggcatcga gatcacctcg gtgaacctcg aggattc
//
LOCUS       DAOMPG010000083         9269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3194_length_9269_cov_4.708270, whole genome shotgun sequence.
ACCESSION   DAOMPG010000083 DAOMPG010000000
VERSION     DAOMPG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_9269_cov_4.708270"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2588)
                     /locus_tag="PLS34_06125"
     CDS             complement(<1..2588)
                     /locus_tag="PLS34_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP87079.1"
                     /translation="MRNNQLRFAVRTALAAGALTTAVAVPAAYAQEEQAADLDRVQVT
                     GSRIQRTDIEGALPITVIDREDIERSGFQSVSDLLRNTTFNSAGSYRPQSGSSFQGLN
                     LIDLRGLGSSRSLVLVDGRRLTMAPQAGSAQDLNSIPLGAVERIEILSDGASAIYGSD
                     AIGGVVNIITRRDFNGAELTIGHGQVSVPKEGGDRDWGSAVFGSSSADTRIIAGVSWN
                     KRDIIFERDFPWVQPGSSIYGNNWFDDTFVAKAIPGGCNEPNFIPTTIGGDPRCQYNF
                     NATNANEASNSLESIFFKADQRINDDWSVFANASVARTKSFGRYAPAPDTNYFYPGYE
                     LGPDAFNNPTNPDAWFYDPNNPNAVAFDPAVVGTGGVADPRTVWSYHRFAAAGNRDSE
                     VDGENLDFMIGAEGTVGMFEVDFGYRRVRNKTYDIGNGYLAAATAWGFVRDFNPGYSP
                     DGTVFDPSLYYYGYDLQNPGSNPANVLAGSVITISRVANFNIDELFGSAAFDIVEIGG
                     GMVQGFFGAERREEVYSDKYDSQSEAGLVGGSAGNSAGGGRDVTAMYFEVLAPITNTF
                     EISLAGRHDDYSDFGTNFSPKISFRFQPLDNLMFRASYGEGFRAPPLDILTQQTSFSA
                     DSVTDPVTCLALNGDATQPCQINAFYIANPDLGAEESKQYALGVAWQPLEWLNMSLDY
                     YNIEIDDRIRSFTAQNLIDRELAGDPIPPGLGVVRNPLGVITSITAGFGNEGTLETDG
                     FDLNVRTNFNFGEFGRLEQNLQVSYINDYSIDGGRSLVKDPGLPEMRLILANAYSWGD
                     FEFAYNLNMIGKQYRSIVAGQGVGSIGSWTTHDVQASYFAPWDGQFTIGVQNLTEKLP
                     EFVAFGG"
     gene            complement(3002..4033)
                     /locus_tag="PLS34_06130"
     CDS             complement(3002..4033)
                     /locus_tag="PLS34_06130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP87080.1"
                     /translation="MARDSKDKQGHRLDRDEWTRYWARGTVTTFEGHFSGNYDAEIRD
                     FWWRQFETLADGSTMVDLATGNGALALLAASYAREHERSFTIVGVDSAALDVARLRAA
                     LPGLAADLDAVTLRGDTPLEDTGLPAATAGLVTSQFGFEYGDTAAGAREAFRLLRSGG
                     RLAMILHHADSAILSQAREGLRQVELCVERERLLAQARLLIKLLRPARRDPSGRRLVL
                     SPGAEKVRDDMLAAVERVRAYAARPEVQTEDTGFIEFMVPAVMRLVDQSHGVDPRRVQ
                     QAMAGLAGEIEAYRRRMTDLVSASRSRADMELVRAQLADAAFADIQLEPLYYGGETLL
                     GWALTATRL"
     gene            4155..4733
                     /locus_tag="PLS34_06135"
     CDS             4155..4733
                     /locus_tag="PLS34_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87081.1"
                     /translation="MKLYRLAVTAATLAAAQLMAVAPAAAQTETVIADQLVLCMQHEE
                     DGARLACFDRLARRATAALEAGTSLGAPVAGVAAARPAQPAAPAATARGPEAVPAAAP
                     EADFGLEKSRAREAARQVDSIRSRVPGLFLGWTGDTVFRLENGQVWKQVESGSFGMRL
                     ENPEVEISRGWFGGYFLSVEGLNRRVRVERVE"
     gene            4920..5468
                     /locus_tag="PLS34_06140"
     CDS             4920..5468
                     /locus_tag="PLS34_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87082.1"
                     /translation="MINAAQRTYEGLRRELDKLTARANKTAERVNDLARRLQDQAQKA
                     VNEGSRAAANQAKRIERMLADARKELDAVSKDQKWLKTEAARAYDYFRRVSGIDRAVA
                     AVEKEWAKRTGAPAKKRVTRKKRKAAAKKAPAKKRATKKRPATGKQAAAKKKPATRKK
                     AATRKKPAAKKKAASRKKAPAA"
     gene            complement(5531..7435)
                     /locus_tag="PLS34_06145"
     CDS             complement(5531..7435)
                     /locus_tag="PLS34_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRP87083.1"
                     /translation="MSLLQSRSSNKTGKRVSIELQKAERALKDCRHQEVEQLCAEVIE
                     ERPDTAQAYQILAELRLRQRRFDEGMAFVEKAREIAAHNPRTLNLYGRVLDHREDLAG
                     AEAAFRHAVEIDPDYADALANLGSVLLRTGRAAEAEPLFRRAIRQDREHGLANLSLGA
                     ILYEQGCPELAVQHLQAGIQRELTNRPGQFTLAIALHELGRLDEAITAYRRLVAAGDQ
                     DPRVYSGLAAALDALGDVDIAMAGYETALELAPDFAPAAAGLADIMTRKGRVHEAFEL
                     LAPLADRGDAPPCLHVSRARALRASGRDDEALLLLADLAKRPGSQAELAPAHFMLGDL
                     LDARGEFDRAFAQYRRARQLRGGRYQPAAHEEFVTRLTRSFTRETMKSLPQGCASDIP
                     VFIIGMPCAGGVLLETIIAAHPRGATAGALPHIDLGAGRLGRYNNAGLSYPECAGVLR
                     ERELRELSAAFMSRLFVGHERKRRIADSMWLNFLHVGLIEIMFPQARLIHCRRSPLDM
                     GLGCYFRDFNEPGDPFAVRLEDVGHYYAQHLRLLDHWREHTSLPLLEVDFEALVNDRT
                     GARRRVIEFLGLPWDPACETAEERSRSPDERASPGLPARETTVGWSRNYEQYLAPLRD
                     GFVAAGCAVD"
     gene            complement(7476..7892)
                     /locus_tag="PLS34_06150"
     CDS             complement(7476..7892)
                     /locus_tag="PLS34_06150"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HRP87084.1"
                     /translation="MNDHQVYAAVAILVVLAMALWLSLRALRRADRLERRQALLEAEL
                     AAAREALARAEAAAGEMSATSERRHAEVDARLAGIEEQQEQLRLLGEGGAGYGQAIRL
                     ARQGATAEDLARDCGLNRGEAELLVSLHGPAGKDQA"
     gene            complement(7889..8095)
                     /locus_tag="PLS34_06155"
     CDS             complement(7889..8095)
                     /locus_tag="PLS34_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87085.1"
                     /translation="MNPFGPLNPVERVWPRRPPGERRERRQEPPGPPAGPEDRDPPAD
                     EPPSERQSGDDKPPSPGRIVDDYA"
     gene            complement(8092..8985)
                     /gene="motD"
                     /locus_tag="PLS34_06160"
     CDS             complement(8092..8985)
                     /gene="motD"
                     /locus_tag="PLS34_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HRP87086.1"
                     /translation="MARRKHKEDHVNHEAWAIPYGDLVTLLLAFFVVMYAVSSVNEGK
                     YRALSDSLVAAFRGAPRTMEPLQVGAFQVRADQELETGLPRTMVPFEVDAPFNPIKDT
                     SERSRYGEAGLGPGDGSGIDPDDSAYVLIQQLEDEVLAAMAPLVDADMISVRRVSYWL
                     EVEINTNFLFASGSATVASTAVPAIQRLAALLKDAGVRLQVEGHTDSVPISTAQFPSN
                     WELSAGRAASVVHLLSGYGIDPATMAAVGYGEFRPVADNESREGRRKNRRVVIIVMSG
                     KAQERELARTGQEGQAAESST"
     gene            complement(8990..>9269)
                     /locus_tag="PLS34_06165"
     CDS             complement(8990..>9269)
                     /locus_tag="PLS34_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP87087.1"
                     /translation="GIIGAVLGLIAVMGNLADPSKLGPGIATAFTATIYGIASANMFF
                     LPVANKLKTAVAAATTYREMLVEGMVSIAQGENPRNIETKLNGYVSAG"
BASE COUNT         1521 a         3113 c         3187 g         1448 t
ORIGIN      
        1 ccgccgaagg cgacgaactc gggcagcttc tcggtgaggt tctgcacacc gatggtgaac
       61 tggccgtccc acggagcgaa gtacgacgcc tgcacgtcat gcgtcgtcca ggagccgatc
      121 gagcccacgc cctggcctgc cacgatcgag cggtactgct tgccgatcat gttcaggttg
      181 taggcgaact cgaagtcccc ccaggagtag gcattcgcca ggatcaggcg catctccggc
      241 aggcccggat ccttcaccag actacgaccg ccgtcgatgc tgtagtcgtt gatgtaggag
      301 acctgcaggt tctgctccag ccgaccgaat tcgccgaagt tgaagttcgt ccggacgttc
      361 aggtcgaagc cgtcggtctc gagggtacct tcgttaccga aaccggcggt gatgctggtg
      421 atcacgccca gcgggttacg caccacgccg agtcccggcg gaatcgggtc gcccgcgagc
      481 tcgcggtcga tcaggttctg cgcggtgaac gaacgaatac ggtcgtcgat ctcgatgttg
      541 tagtagtcca gggacatgtt cagccactcg agcggctgcc aggccacgcc gagggcgtac
      601 tgcttggact cctcggcacc gaggtccggg ttggcgatgt agaacgcgtt gatctggcag
      661 ggctgagtgg catcgccgtt cagcgcaagg caggtcaccg ggtcggtcac cgagtcggcc
      721 gagaacgagg tctgctgggt caggatgtcc agcggcggcg cgcggaagcc ttcgccgtag
      781 gacgcccgga acatcaggtt gtcgagcggc tggaagcgga agctgatctt cggcgagaag
      841 ttggtgccga agtcggagta gtcgtcatga cgaccggcga gcgagatctc gaaggtgttg
      901 gtgatgggcg ccagcacctc gaagtacatc gccgtcacgt cacgaccgcc gcccgcggag
      961 ttgcccgcag aaccgccgac caggccagcc tcggactgcg agtcgtactt gtcgctgtag
     1021 acctcttcac gacgctcggc gccgaagaag ccctgcacca tgccgccgcc gatctcgacg
     1081 atgtcgaacg ccgccgagcc gaacagctcg tcgatgttga agttcgccac tcgagagatg
     1141 gtgatgaccg agccggccag cacgttcgcc gggttggagc ccgggttctg caggtcgtag
     1201 ccgtagtagt acagggaggg atcgaacacc gtgccgtccg gcgagtagcc ggggttgaag
     1261 tcacgcacaa agccccacgc cgtggcggcg gccaggtagc cgttgccgat gtcgtaggtc
     1321 ttgttgcgga cgcggcggta gccgaagtcc acctcgaaca tgcccacggt gccctcggcg
     1381 ccgatcatga agtcgaggtt ctcgccgtcc acctcggagt cgcgattgcc ggccgcggcg
     1441 aagcgatggt agctccacac ggtgcgcgga tccgccacgc cgcccgtgcc gacgaccgcc
     1501 gggtcgaacg ccacggcgtt cgggttgttc gggtcgtaga accaggcgtc cgggttggtc
     1561 gggttgttga acgcatcggg acccagctcg tagcccggat agaagtagtt ggtgtccggg
     1621 gcgggcgcgt aacgaccgaa ggacttggtg cgcgccacgc tggcgttggc gaacacggac
     1681 cagtcgtcgt tgatgcgctg gtcggccttg aagaagatcg actccaggct gttcgacgcc
     1741 tcgttggcgt tggtcgcgtt gaagttgtac tggcaacgcg ggtcgccgcc gatggtcgtc
     1801 gggatgaagt tcggctcgtt acagccgccc ggaatggcct tggcaacgaa agtgtcgtcg
     1861 aaccagttgt tgccgtagat ggacgaaccg ggctggaccc acgggaagtc gcgctcgaag
     1921 atgatgtcgc gcttgttcca ggagacgccg gcgatgatcc gggtgtcggc gctcgaggaa
     1981 ccgaacacgg ccgaacccca gtcacgatcg ccgccttcct tcggaacgct gacctggccg
     2041 tggccgatcg tgagctcggc gccgttgaag tccctgcggg tgatgatgtt gaccacgccg
     2101 ccgatcgcgt cggagccgta gatggccgag gcgccgtcag acaggatctc gatgcgctcc
     2161 accgcgccca gcgggatgga gttcaggtcc tgcgcggaac cggcctgcgg ggccatggtc
     2221 aggcgacggc cgtcgaccag caccagggag cgcgaggagc ccaggccgcg caggtcgatc
     2281 aggttgaggc cctggaacga ggaaccggac tgcggacggt aggaaccggc gctgttgaag
     2341 gtcgtgttgc gcagcaggtc ggaaaccgac tggaagcccg aacgctcgat gtcttcccgg
     2401 tcgatgaccg tgatgggcag cgcgccctcg atgtcggtac gctggatgcg cgagccggtg
     2461 acctggacgc gatcgaggtc ggctgcctgc tcttcctgcg cgtaggccgc aggcacggcc
     2521 acggccgtgg tgagcgcgcc ggccgcgagt gccgtccgca ccgcaaacct aagctggtta
     2581 tttctcatgt aagaatttcc tccgaagtgt acaaagcttc cgctccgcgg ttgcgacgat
     2641 gcgcagatcc cctgagatgc ggactcccct tgaaatcaag tgtcattggc gcgcccgatc
     2701 gctatgcgcg cggccacgcc acaacacccg tggccggaga gtatcgcaac ttgttgccgg
     2761 atgccaacat ctcgatcaac gacgagttcg cgcctgaggc gctgacgaga cctggccgcg
     2821 cagcaccggg gcgccccaaa tgctcttgtt aaaggcgccg caaggctgct gcacaacggt
     2881 tcgggtgctg cggcgcgacg gcgaggggtt gccaaccagg cctgtggtat tttcgcaacg
     2941 atcaggcgca aaagaactgt tgtggattaa caacaatcgg ggcgggggcc gccccggcag
     3001 gctacagccg ggtggccgtg agcgcccagc ccagcagcgt ctcgcccccg tagtacaagg
     3061 gctcgagctg aatgtcggcg aacgcggcgt ccgccagttg cgccctgacg agttccatgt
     3121 cggcacgact gcgcgaggcg gacaccaggt cagtcatgcg ccgccggtag gcttcgattt
     3181 cacccgccag tccggccatc gcctgctgca cccgccgcgg atcgaccccg tgggactggt
     3241 ccaccagccg catcaccgcg gggaccatga actcgatgaa gccggtgtcc tccgtctgca
     3301 cctcgggtcg ggccgcatag gcgcggacgc gctcgacggc ggcaagcatg tcgtcgcgca
     3361 ctttttccgc acctggcgac aggacgagac gacgcccgct cgggtcgcgg cgcgccggtc
     3421 gcagcagctt gatgagcagg cgcgcctgcg ccagcagccg ctccctctcg acgcacagct
     3481 cgacctgccg gagtccctcg cgagcctgcg agaggatcgc ggaatcggcg tggtggagga
     3541 tcatcgccaa ccggccgccg ctgcgcagca gcctgaaggc ctcgcgcgcc ccggcggcgg
     3601 tgtcgccata ttcgaaaccg aactggctgg tgaccagccc ggccgtcgca gccggcagcc
     3661 cggtgtcctc gaggggcgta tcgccgcgca gcgtgaccgc atcgaggtcg gctgcaaggc
     3721 cgggcaacgc cgcgcgcagc cgggcaacgt cgagcgcggc gctgtcgacg ccgacgatgg
     3781 tgaaactccg ctcgtgttca cgggcgtagc tcgccgccag caacgccagc gcgccgttgc
     3841 cggtggcgag atcgaccatg gtcgatccgt cggccagcgt ctcgaactgg cgccaccaga
     3901 aatccctgat ctcggcgtcg tagttgccgc tgaagtggcc ctcgaaggtg gtcacggtgc
     3961 cgcgggccca gtaccgggtc cactcgtccc ggtccagccg gtgcccttgc ttgtccttcg
     4021 agtctcgcgc catcccgccc tccggccgcc ctgctgacgg cggtcgatcc gccggtcacc
     4081 ggcgcaactt tagagcatgc ggccgcgccg cgtctgctaa gctttccgca gtcgtattga
     4141 ggatgcgtcg aggaatgaag ctttaccgtc ttgccgtcac cgccgccacg ctggccgctg
     4201 cccagctgat ggccgtagcg cccgccgcgg cccagaccga aaccgtgatt gccgaccaac
     4261 tggtgctgtg catgcagcac gaggaggacg gcgcgcgcct ggcctgcttc gaccgccttg
     4321 caaggcgcgc cacggccgcg ctggaggccg gcacgtccct cggcgcgccg gtggcgggcg
     4381 tggccgcggc gaggcccgca caaccagcgg cacccgcggc gaccgcgcgc ggcccggagg
     4441 ccgtgcccgc tgcagcgccg gaggccgact tcggcctgga gaaatcccgg gcacgggagg
     4501 ccgcgcggca ggtggactcg atccgcagcc gcgtgcccgg cctgttcctc ggctggaccg
     4561 gcgacaccgt cttccggctc gagaacggcc aggtctggaa acaggtcgag tcaggcagtt
     4621 tcggcatgcg cctggagaat cccgaggtgg agatttcacg cggctggttc ggcggctatt
     4681 tcctctccgt cgaaggcctg aaccgccgcg tgcgcgtcga acgcgtggag tgaaatccgg
     4741 cgcgatcgcc gccgcttgat tgcggaaatc cgaagcgcgt ttcctttgga agacgctcat
     4801 ccgctggact atagtgagcg gtagtcggcg caccgcgcgc ccatgcacca cgcaaaggta
     4861 atcccatgga cagacagctt tccgaaattg aaaaggcgtt ccaggacgcg cggcaggagt
     4921 tgatcaacgc cgcgcagcgc acctacgagg gactgcggcg cgagctggac aagctgaccg
     4981 cgcgcgccaa caagaccgcc gagcgggtca acgacctggc caggcgcctt caggatcagg
     5041 cccagaaggc cgtcaacgag ggctccaggg cggctgcgaa ccaggccaag cggatcgagc
     5101 ggatgctcgc cgatgcacgc aaggaactgg acgcggtcag caaggaccag aagtggctga
     5161 agaccgaggc cgctcgcgca tacgactact tccgtcgcgt cagcggtatc gaccgtgccg
     5221 tcgcggccgt ggagaaggaa tgggcgaagc gcaccggtgc gccggccaag aagcgcgtga
     5281 ccaggaagaa gcgcaaggcc gccgcgaaga aggcgccggc gaagaagcgc gccacgaaga
     5341 agaggccggc gaccggcaag caagccgccg cgaaaaagaa gccggcgacc aggaagaaag
     5401 ccgcgaccag gaaaaagccc gcggcgaaga agaaggctgc cagcaggaag aaggctcccg
     5461 cagcctgaac cgctggattc cggcccggcc aagcggcggc cccagggtcg ccgctttgtt
     5521 cttgcccgga tcagtcgacc gcgcagccgg cggcgacgaa gccgtcgcgc aacggggcaa
     5581 ggtactgctc gtagttccgc gaccagccga cggtcgtctc cctggccggc aggccgggtg
     5641 aagcacgctc gtccggcgac ctcgaccgct cctcggcggt ctcgcaggcg gggtcccagg
     5701 gcagtccaag gaattcgatc acgcgccgcc gcgcgcccgt gcgatcgttc accagggctt
     5761 caaagtcgac ctccagcagc ggcagcgagg tgtgttcccg ccagtgatcc agcagccgca
     5821 ggtgctgcgc atagtagtgc ccgacatcct ccagccgaac ggcgaacggg tctcccggtt
     5881 cgttgaagtc gcggaagtaa cagcccagcc ccatgtccag cggggacctg cggcaatgga
     5941 tcagccgcgc ctgcgggaac atgatctcga tcagccccac gtgcaggaag ttcagccaca
     6001 tggaatcggc gatgcgcctc ttgcgctcat gaccgacgaa cagccgtgac atgaaggccg
     6061 ccgacagctc gcgcagctcg cgttcgcgca gcacgccggc gcactcgggg tacgagagtc
     6121 cggcattgtt gtagcgcccc aggcggcctg ccccgaggtc gatgtgcggc agcgcgccgg
     6181 cggtcgcccc gcgcggatgc gccgcaatga tggtttccag cagcacgccg ccggcgcagg
     6241 gcatgccgat gatgaacacc gggatgtccg aggcgcagcc ctgcggcaag gacttcatcg
     6301 tttcgcgcgt gaaggagcgg gtcaggcggg tcacgaactc ctcgtgtgcc gccggctggt
     6361 aacgcccgcc acgcagctgg cgggcgcgtc ggtactgggc gaaggcccga tcgaactcgc
     6421 cgcgcgcgtc cagcaggtca cccagcatga aatgcgccgg ggcgagctcg gcctggctgc
     6481 cgggccgctt cgccaggtcc gccagcaaca gcagcgcctc gtcgtcccga ccgctggcac
     6541 gcaaggcacg cgcccgggaa acgtgcaggc agggcggtgc atcgcctcgg tccgccagcg
     6601 gcgccagcaa ctcgaaagcc tcgtgcaccc gacctttgcg cgtcatgatg tccgccagcc
     6661 cggcggcggc cggagcgaag tccggcgcca gttccagcgc agtctcgtag cccgccatcg
     6721 cgatgtcgac gtcgcccagc gcgtccagtg ccgcggcgag accgctgtag acgcgcggat
     6781 cctggtcacc ggcggccacc agccggcgat aggcggtgat cgcctcgtcc aggcggccca
     6841 gctcatgcag cgcaatggcc agcgtgaact ggcccggacg gttggtcagt tcccgctgga
     6901 tgccggcctg caggtgctgc accgcgagtt ccgggcatcc ctgctcgtag aggatggcgc
     6961 ccagcgagag gttcgccagg ccgtgctcac ggtcctggcg aatggcccgg cggaacagcg
     7021 gctctgcctc ggcggcgcga ccggtgcgca acagcacgct gccgagattg gccagcgcat
     7081 cggcatagtc cgggtcgatt tcgaccgcat ggcggaaagc cgcctcggcc ccggcaaggt
     7141 cctcgcggtg gtcgagcacg cgtccgtaca ggttgagcgt gcgcggattg tgcgcggcga
     7201 tttccctggc tttctcgacg aacgccatgc cctcgtcgaa gcggcgctgc cggagccgca
     7261 gctcggcgag aatctggtag gcctgggcgg tgtccgggcg ctcctcgatc acttccgcgc
     7321 agagctgctc cacttcctgg tggcggcagt ccttgagcgc ccgttcggcc ttctgcagtt
     7381 cgatggagac ccgcttcccg gtcttgttgc tggagcggct ttgcaggagg ctcatcatgg
     7441 gttcgtgctc agggtcgtgg cgcgcggcgc ggcggctacg cctggtcctt gcccgcgggg
     7501 ccgtgcagcg agacgagcag ctccgcctca ccgcggttca ggccgcaatc cctggccagg
     7561 tcctcggcgg tggcgccctg ccgcgccagc cggatggcct ggccgtaccc ggcgccgccc
     7621 tcgccgagca gccgcagctg ttcctgttgt tcctcgatcc ccgccagccg ggcatcgacc
     7681 tccgcatggc ggcgctcgct tgtggcggac atctcaccgg cggccgcctc ggcgcgtgcc
     7741 agcgcctccc gggccgctgc cagctcagcc tcgagcagcg cctgtcgtcg ttccagccga
     7801 tccgcgcggc gcagggcgcg caacgagagc cacagcgcca tggccaggac gacgaggatt
     7861 gcgacggcgg cgtaaacctg gtgatcgttc atgcgtagtc atccacgatg cggccggggg
     7921 acggcggctt gtcatcgccg gattgtcgct cagatggtgg ctcatccgca ggcggatcgc
     7981 gatcctccgg tcccgccggc gggccgggcg gctcctggcg ccgctcgcgg cgctcaccgg
     8041 gcggccgacg cggccagacc cgttccaccg ggttgagcgg gccgaacggg ttcacgtgct
     8101 ggactccgcc gcctgcccct cctgccccgt cctggcaagc tcgcgctcct gcgccttgcc
     8161 ggacatgacg atgatcacga cgcgccggtt cttgcggcgt ccctcgcggc tctcgttgtc
     8221 cgcgaccggc cggaactcgc catagcccac ggcggccatg gtcgccggat cgatgccgta
     8281 gcccgacagg agatggacga cgctcgccgc acgcccggcc gacaattccc agttggacgg
     8341 gaactgcgcc gtgctgatcg gcaccgagtc ggtgtggccc tcgacctgca ggcgcacgcc
     8401 cgcgtccttg agcagcgcgg cgagccgctg gatcgccggc accgccgtgg atgccaccgt
     8461 ggcgctgccg ctggcaaaca ggaaattagt gttgatctcg acttccagcc agtaggagac
     8521 gcgccgcacg ctgatcatgt ccgcgtcgac cagcggcgcc atggccgcca acacctcgtc
     8581 ctccagttgc tggatcagga cgtaggcgct gtcgtcgggg tcgatgcccg agccgtcgcc
     8641 ggggccgagc ccggcttccc cgtagcggct gcgctcactg gtgtccttga tgggattgaa
     8701 gggggcatcc acctcgaagg gcaccatggt gcggggcagg cccgtctcga gctcctggtc
     8761 ggcgcgcacc tggaaggcgc ccacctgcag cggctccatg gtgcgtggcg cgccccgaaa
     8821 cgccgccacc agcgagtcgg acagcgcccg atacttgccc tcgttcaccg acgacacggc
     8881 atacatgacc acgaagaagg ccagcagcag ggtcacgagg tcgccgtagg ggatcgccca
     8941 ggcctcgtgg ttgacgtggt cctccttgtg tttacggcgc gccatagaat caaccggcgc
     9001 tcacgtagcc gttcagcttg gtttcgatgt tgcgcggatt ttcgccctgg gcgatcgaga
     9061 ccatgccttc gacgagcatc tcgcgatacg tggtcgccgc cgccacggcc gtcttgagct
     9121 tgttggcgac cggcaggaaa aacatgttgg cgctggcgat gccgtagatg gtggccgtga
     9181 aggccgtcgc gatgccgggc cccagcttgc tcgggtcggc gaggttgccc atcaccgcga
     9241 tcagtcccag gaccgcgccg atgatgccg
//
LOCUS       DAOMPG010000084         9025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3314_length_9025_cov_3.953177, whole genome shotgun sequence.
ACCESSION   DAOMPG010000084 DAOMPG010000000
VERSION     DAOMPG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_9025_cov_3.953177"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLS34_06170"
     CDS             complement(<1..253)
                     /locus_tag="PLS34_06170"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP87088.1"
                     /translation="MWPLLAVGGLALILQLFGGAASWRLERDLVLAEPWRLVSAHLVH
                     LGWAHLALNLVGALLVWGLVGRAFTAGQWVLLMVACAAGV"
     assembly_gap    490..503
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<504..1649)
                     /locus_tag="PLS34_06175"
     CDS             complement(<504..1649)
                     /locus_tag="PLS34_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP87089.1"
                     /translation="MQSRFMTAGALALIGLTAYAAPAAADDVFCPPNLGVETIDGNVI
                     VTGACVLDSTRVIGNVHIYPGGSLVARNTHIDGNIQAEGADFVDVRNSRVGGNVQLDD
                     LVSGATRIELVRVDGSIQLTGNRVSLVVLDNIVGSDVQAFGNSGGVDITDNTIDGNLQ
                     CRENVPAPTGSGNQVSGNSEDQCANLVPSDGSGGGSGDGSGGGTGAIDGDVNCPPNLG
                     RVTVDGNVLVTAACRLDGTVVKGNVLLYSGGSLVARDARVDGNIQADRADYIDVARVT
                     VKGNIQLDDMVGDLSEIRGSGVDGNIQLVGNRATLEVVDNVVDGDVQAFSNTGGLLIA
                     DNVIGGNLQCRSNQPAPVGGNNMVSGNREDQCADLQGGRSADVRSGGS"
     gene            complement(1690..3222)
                     /locus_tag="PLS34_06180"
     CDS             complement(1690..3222)
                     /locus_tag="PLS34_06180"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HRP87090.1"
                     /translation="MNTRITHAVIFVLTAMLLGACGGGSDPAAGVPGGGGSGGGSGGG
                     SGGGGSGGGTGGGTGGGPDDPGDPLFGINGGGAWVVSGAVSRFSSVVVNGVRFDATDS
                     EIVVNGQPATQAGLAQGQVVAVRGEVAIGGLEGTAERVEYENSVRGSIRELDPGARTA
                     DVLGQRIRAGLETIYGPSIGPDGLEALGSGDAIVVSGYRDSSGRLDATRIDRDPLPGT
                     AEVRGVAREVDPVALSLRINDLRVDFSAAVLDGFASGMPVPGDTVFVRGPEPAPGESL
                     RAGLLAWRPAAVDIAADENVDLEGLVTRFESLGDLDVAGQRVVIVAETEFVASTDQEV
                     ARDAFVDVEGRVDADGRLLVARMQVIPRADIDIEARIDGVDVAAGTIQLLGIEVFIDA
                     RTRFEEDDGLQLELADLRPGDGVEIAGYYREGRFTAVRVARDDDDDEVEIEGPAMDLA
                     QPEFRIGGIRIVTDALTDFDGDDGPITGDEFFSIAEGRRVEVEGSWNGEFVRAEEVEL
                     ED"
     gene            complement(3219..4046)
                     /locus_tag="PLS34_06185"
     CDS             complement(3219..4046)
                     /locus_tag="PLS34_06185"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HRP87091.1"
                     /translation="MASGTKSHVFAACRRLLRPVVRLMLKAGVNWKEFSDLSKTVFVD
                     VATGEFGLRGRPTNATRVAMLTGINRHEVRRQREQLADTSEPAPTYLNAAQRVLSGWH
                     QDADYQAEGKARAIPESGTGPSFQELCRRYGGDMPATALLRELRRVGAVVENRDGLLE
                     ARARTYIQARLDPEKILRAGSVMEDLGDTVVFDLTAPESARLRFERRAENSMIERRHV
                     AAFQDFLEQEGMAFLERIDDWLTRHETRAGKPGGTVPVRLGVGVYHIENQPLRGPRP"
     gene            complement(4282..4758)
                     /locus_tag="PLS34_06190"
     CDS             complement(4282..4758)
                     /locus_tag="PLS34_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87092.1"
                     /translation="MRIAETIPTTASIGLAFLLATLLLPGCTALDTAGMKPACARNCA
                     VTITVPRDPAQPPMVDIEKFHLKARSSLRFIVKFEDEADSGTASVTLEFDRDPFEAHK
                     GNPGKPLPVQANRDNVFKSRPYSYRQCPANDACKYDVVVQRPEGESRLDPWIIIER"
     gene            complement(4780..6765)
                     /locus_tag="PLS34_06195"
     CDS             complement(4780..6765)
                     /locus_tag="PLS34_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP87093.1"
                     /translation="MSGRASAVNRVLRELRRRHVFRLAGVYLVGSWLILQVADVLLDI
                     IGGPEGSLRVIALVLALVFPMAMIMAWVYDLTPEGVVRTGATGEEDAPAFRWDWRWVD
                     YAIIAALVAILVFVLADKRSAQEPGLAAPAHSVAVLPFSDLSPDGDHRYFSDGLSEAL
                     MDSLSRIPGLQVASRTASFAYRDPGPAVREVARSLEVATLLEGSVRKSGDQLRISARL
                     VDGGSGRRLWSESYDASLEDVFAVQDTISRSIAEVLKIRLLGGEVLVEVPTRDQVAYE
                     DYLRGRDQLRREGTVVQLELAAEHFRRALARDGEFSLALAGMCTALWEKYSITLDGEL
                     AERAIEACKDAEGQVDPPAETLVALGGLYRGRGELEKSLALLSRARAAAPNDADVQSG
                     LGDTLRAMGDFDGAIAHQRRAIELDPAFWRHHWSLGVSLLRSGSLDEAEAQFNRAIRL
                     QPESPFPYSSLGAVYFFRGEYLKAGDAFRESIRRNPNPQALSNAGTNYFYAGDYVQAE
                     EMFRQAATMSPSDYRYRGFVAEAVDMQGTRDAAEVAGYYEAAVMKVREQLAINPGDHV
                     GRAALASYLARLDRTAAAEAELATLQREQGLNVEALRTMAMAYLFLGRREAAIEHFAA
                     AVAQGFPAALLTLDPRLQVLEDMKEFQALLTRNAADN"
     gene            complement(6893..8836)
                     /locus_tag="PLS34_06200"
     CDS             complement(6893..8836)
                     /locus_tag="PLS34_06200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRP87094.1"
                     /translation="MLIRMLLPVALSLALSSPLHASAERVERGNLVLENLPEIPAEIT
                     ERLEQYQNTRAAGFGSWLHDGSMLISTRFGDTNQVHRVRQPLGARQQLTFFKEPINTV
                     SVSPDPALRGFIYARDIGGNEFFQLYWLDLDTSESRLLTDGRSRNTGPVWSNRGDRFA
                     YASTRRDGRNYDIYVATPAGGHETHLLALEGSGLWVPMDWAPDDSRLIVINYRSITDA
                     EVHVVDLESGAVQQVNPQDGPVGYAAGAFDRTGKGVYLVHDHGTEFKQLHHFDLASGN
                     SRPLSAHIPWDVNMFTLSRDRETMAFVVNAGGMAELHLLDLRRKRALPAPELPVGLIG
                     GLGFSPDGRALAMVLNTPQSPSDVYVYGLRKRDLVRWTESEVGGLDTARFPVPELVTF
                     TSFDGLEVPAWVYRPVQAGPRPVIVQIHGGPEAQSRPAFNSLFAYWVNELGAAVVSPN
                     VRGSAGYGKSYLQLDNGVLREDSVRDIGALLDWIATQPDLDEERVIVYGGSYGGYMVL
                     ASLVHYDDRLLGGVSIVGISNFVTFLENTEAYRRDLRRVEYGDERDPAMREFLEGISP
                     LNNAEQISSPLFVAQGFNDPRVPYTESEQIVREVRGNGGEVWYLLAMDEGHGFAKKPN
                     RDYFQAATVMFFREQFGSVGDPQ"
     gene            complement(8870..8956)
                     /locus_tag="PLS34_06205"
     tRNA            complement(8870..8956)
                     /locus_tag="PLS34_06205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8920..8922),aa:Leu,seq:taa)
BASE COUNT         1459 a         3199 c         2903 g         1450 t
ORIGIN      
        1 cgaccccggc cgcacaggcg accatcagca ggacccactg ccccgccgtg aacgcacgcc
       61 ccaccaggcc ccacaccagc agggcgccca ccaggttcag ggccaggtgc gcccagccca
      121 ggtggaccag gtgcgcgctt accaatcgcc agggctcggc cagcacgaga tcgcgttcca
      181 gtcgccagga cgctgcgccg ccaaacaatt gcaagatgag cgcgaggccg ccgactgcaa
      241 gcagtggcca cacttcagag aggcttgtcc tgatgttgcg caatggtcgg tccgtacgcc
      301 gggccagggc gcctggtaaa ggagttcgcg gccccgggag gatacagggc cgcgaactcg
      361 ccatgtaccc ggcgggcatc aggccggccg gctgcccggg gagcggcgca tgaaggcggc
      421 tgcgccaagc agcaggagca tgagcggacc ggtgccgccg ccgccggagg cctccgtcgt
      481 ggtcgagccn nnnnnnnnnn nnnactgccg cccgagcgca cgtccgcact gcggccgccc
      541 tgcaggtcag cgcactggtc ctcgcggttg ccggagacca tgttgttgcc gccgaccggc
      601 gccggctggt tgctcctgca ctgcaggttg ccgccgatca cgttgtcggc gatcaacagc
      661 ccgccggtat tgctgaaagc ctggacgtcg ccatcgacga cgttgtcgac cacctccagc
      721 gtcgcacggt tgccgaccag ctggatgttg ccgtccacgc cgctaccgcg gatctcgctg
      781 agatcgccga ccatgtcgtc aagctgaatg ttgcccttga cggtcacccg tgcaacgtcg
      841 atgtagtcgg cacgatcggc ctggatgttg ccgtcgaccc gggcgtccct ggcgaccagc
      901 gagccgccgg aatacagcag cacgttgccc ttgacgaccg tgccgtcgag ccggcaggcg
      961 gccgtgacca gtacgttgcc gtcgaccgtg acgcgtccga ggttcggcgg gcaattgacg
     1021 tcgccgtcga tggcgcccgt gccgccgcct gagccgtcgc ctgagccgcc gcctgagccg
     1081 tcggacggca ccaggttggc gcactggtcc tcgctgttgc cggacacctg gttgccgctg
     1141 ccggtaggtg cgggaacgtt ttcccggcac tgcaggttac cgtcgatggt gttgtcggtg
     1201 atgtccacgc cgccggaatt accgaaggcc tgcacgtcgc tgccgacgat gttgtcgagc
     1261 accacaaggc tgacgcgatt gccggtcagc tggatgctgc cgtcgaccct gaccagctcg
     1321 atgcgggtgg cgccgctcac aaggtcgtcg agctggacgt tgccgccgac gcgcgagtta
     1381 cgtacgtcta cgaagtcggc tccttctgcc tggatattgc cgtcgatgtg cgtgttgcgc
     1441 gcgaccagcg agccgcccgg gtagatatgc acgttaccga tcacccgcgt gctgtcgagc
     1501 acgcaagcgc cggtcacgat cacgttgcca tcgatggtct cgacgccgag gttcggcggg
     1561 cagaacacgt catcggccgc cgcgggcgcc gcgtaagccg tgagtccgat cagcgccagt
     1621 gcgcctgcgg tcatgaatct cgattgcatg tcattgctcc tggcattgtc tttgaatatg
     1681 aagcggtgtt cagtcttcga gctcgacttc ctcggcgcgc acgaactcgc cgttccagga
     1741 gccctccact tccaccctgc ggccctcggc gatgctgaag aattcgtcgc ccgtgatcgg
     1801 gccgtcgtcc ccgtcgaagt cggtcagcgc gtcggtcacg atgcggatcc cgccgatgcg
     1861 aaactccggc tgggccaggt ccatggctgg gccctcgatt tcgacctcgt cgtcgtcatc
     1921 gtcccgcgct acgcgcacgg ccgtgaaacg gccctcccgg tagtagccgg cgatctccac
     1981 gccgtcgccc gggcgcaggt cggcgagttc gagttgcagc ccgtcgtctt cttcgaagcg
     2041 cgttcgcgca tcaatgaaca cctcgatacc cagcagctgg atggtgccgg cggcgacatc
     2101 cacgccgtcg atgcgtgctt cgatgtcgat gtcggcgcgc ggaatgacct gcatgcgggc
     2161 caccagcagt cggccgtccg catcgacccg cccctcgacg tcgacgaagg cgtcgcgggc
     2221 cacttcctgg tccgtcgagg ccacgaactc cgtctccgcc acgatcacca cccgctgacc
     2281 cgccacgtcc aggtcgccca gggattcgaa gcgcgtcacc agcccttcga ggtccacgtt
     2341 ctcgtccgcg gcgatgtcca ccgccgccgg ccgccacgcc agcagtccgg cccgcaacga
     2401 ctcgcccggg gccggctccg gcccgcgcac gaacaccgtg tcgcctggta cgggcatacc
     2461 cgacgcaaat ccgtccagga cggctgcgga gaagtccacc cgcaagtcgt tgatgcgcag
     2521 actgagcgcg accggatcga cctccctggc gacgccccgg acttccgccg tgccgggcaa
     2581 cggatcgcga tcgatccggg tggcatccag ccggccggag gaatcccggt agccgctgac
     2641 cacgattgcg tcgccggacc cgagggcctc gaggccgtca ggcccaatgg atggcccata
     2701 aatggtctcc agccccgcgc ggatgcgctg gccgagaacg tccgcggtgc gtgcgccggg
     2761 atcgagctcg cgaatcgatc cgcgtacgct gttctcgtac tcgacgcgct ccgcggtgcc
     2821 ttccagcccg ccgatggcga cctcaccgcg caccgcgacc acctggccct gcgccaggcc
     2881 ggcctgggtc gccggctggc cgttgacgac gatctcgctg tccgtggcgt cgaatcgcac
     2941 cccgttaacg accacgctgc tgaagcgcga cacggccccg gacacgaccc aggcgccgcc
     3001 gccattgatg ccgaacaacg gatcgccggg atcatccggc ccgccacctg tgccgccgcc
     3061 ggtgccgccg ccggacccgc cgccgcccga gccgccgccc gagccgccgc ccgagccgcc
     3121 acccccgggg accccggcgg ccgggtcgga accgccgcca caggctccca gcagcatggc
     3181 cgtcaggacg aagatcacag cgtgcgtgat ccgggtattc atggtctggg tcctcgcagg
     3241 ggttggttct cgatgtgata aacgccgacg ccgaggcgca cgggtacagt gccgccgggc
     3301 ttgccggccc tggtctcgtg gcgcgtcaac cagtcgtcga tgcgctccag gaaagccatg
     3361 ccctcctgtt cgagaaagtc ctggaacgcc gcgacgtggc gccgctcgat catgctgttc
     3421 tctgctcgcc gctcgaatct caggcgggcg ctctccggcg cggtgaggtc gaacacgacc
     3481 gtgtcaccca ggtcctccat gacgctgccg gcccgcagga tcttttcggg atccagcctt
     3541 gcctggatgt aggtgcgcgc ccgggcctcc agcaatccat cccggttctc gacgaccgcc
     3601 ccgacacgac gaagctcacg caacagcgcc gtcgcgggca tgtcgccgcc gtaacgccgg
     3661 caaagctcct ggaaggaagg cccggtcccg gactcgggta tcgcacgtgc ctttccctca
     3721 gcctggtagt ccgcgtcctg gtgccagccc gacagcacgc gctgggcggc gttcaggtag
     3781 gtcggcgccg gctcactcgt atcagccagc tgctcgcgct ggcggcggac ttcgtgccgg
     3841 ttgatgccgg tgagcatggc cacgcgcgtc gcattggtgg gccttccgcg caacccgaac
     3901 tccccggtgg caacatcgac gaataccgtc ttggacaagt ctgagaactc cttccagttg
     3961 acgccggcct tgagcatcag gcgcaccacg ggacgcaaca gccgccggca agcagcgaac
     4021 acatgcgact tcgtaccgga tgccatatct gtttgcattg aaaccaaata tagctaccct
     4081 gaccgcggct cgcaagtccc gcgcacaatg gaccggcggc cggcggcacc gccggtcaag
     4141 aactcgagca acaacagcgg attaggcgcg cgccccgctg gcgcacgccc ggcagcgtgc
     4201 cgcccgaacc gcgcgttcgg gcctgttccg gtcactgccc ggtcgccggg ctcgcccggc
     4261 aagccggcgg atccggactg cttacctctc tatgatgatc cagggatcga gacgggattc
     4321 cccctcgggg cgctgcacga ccacgtcgta cttgcaggcg tcatttgccg ggcactgccg
     4381 ataggagtag ggccgtgact tgaagacatt gtcacggttc gcctgcaccg gcagcggctt
     4441 cccgggattg cccttgtgcg cctcgaacgg atcccggtcg aattcgaggg taaccgatgc
     4501 cgtgccggag tccgcctcgt cttcgaactt cacgatgaat cgcagcgaag accgcgcctt
     4561 gaggtgaaac ttctcgatgt ccaccatcgg cggctgggcg ggatcgcgcg ggaccgtgat
     4621 ggtcacggcg cagtttcgcg cgcacgcggg tttcatccct gccgtgtcca aggcggtaca
     4681 ccccggcagg agcagcgttg caagcaaaaa ggccagcccg atgctggcgg tcgtcgggat
     4741 cgtctcggca attctcatga ctctctctcc catggcagct caattgtcgg ccgcatttcg
     4801 tgtcagcagt gcctggaact ccttcatgtc ttcgagaacc tggaggcgcg gatccagcgt
     4861 cagcaatgca gccgggaagc cctgggccac cgctgccgca aagtgctcga ttgcggcctc
     4921 gcggcggccg aggaacaggt aagccatcgc catggtgcgc aacgcctcca cgttcagccc
     4981 ctgctcgcgc tgcagtgtgg caagttcagc ttccgccgcc gctgttctat cgaggcgcgc
     5041 caggtagctg gcgagcgcgg cccggccgac gtgatcgccg ggattgattg ccagctgttc
     5101 ccgtaccttc atcactgcgg cctcgtaata cccggcgacc tcggccgcat cgcgcgtccc
     5161 ctgcatgtcg acggcttccg ccacgaagcc gcggtaacga taatccgacg gactcatcgt
     5221 cgcggcctgg cggaacattt cctcggcctg gacatagtcg ccggcataaa aatagttggt
     5281 gcccgcgttc gagagggcct ggggattcgg attacgccgg atggactcgc ggaaggcgtc
     5341 gccggctttc aagtactcgc cgcggaaaaa gtagaccgca ccgagactgg agtaagggaa
     5401 ggggctctcg ggctgcaagc ggatggcgcg attgaactgc gcctccgcct cgtcgaggct
     5461 gccgctcctc aacaggctga cgcccaggct ccagtgatgg cgccagaacg ccggatccag
     5521 ctcgatcgcc cgccgctggt gcgcgatggc gccgtcgaaa tcgcccatcg cccggagcgt
     5581 gtcgccgagg cccgattgca cgtcggcatc gttcggcgcc gccgcgcgcg cgcggctcaa
     5641 cagcgccagc gacttctcca gctcgccgcg gccacggtaa agtccgccca gcgccaccag
     5701 cgtctcggcc ggcgggtcga cctggccctc ggcatccttg caggcctcga tcgcccgctc
     5761 cgccagctct ccgtcaaggg tgatgctgta tttctcccac aacgccgtgc acatgccggc
     5821 cagggccagg ctgaactcgc cgtcccgcgc cagtgcccgg cgaaagtgct ccgcggccag
     5881 ctccagctgg acgacggtgc cctcgcggcg cagctggtcc ctgccccgca ggtagtcctc
     5941 gtaggcgacc tggtcgcggg tcggcacctc caccagcact tcgccgccga gcagcctgat
     6001 cttgagcact tcggcgatgc tgcgggaaat cgtgtcctgc acggcgaaga cgtcttccag
     6061 gctggcgtcg tagctctcgc tccacaggcg ccgcccgctg ccgccgtcca ccaggcgggc
     6121 gctaattctc aactggtcgc cggactttcg cacgctgccc tccagcagcg tggccacctc
     6181 tagcgagcgc gcgacttcgc ggaccgcggg acccgggtcg cgataggcga acgaggccgt
     6241 ccgcgacgcc acctgcagcc cgggaattcg cgacaggctg tccatcagcg cctccgacag
     6301 gccgtcgctg aagtaccgat gatccccatc cgggctgagg tccgaaaacg gcagcaccgc
     6361 cacggaatgc gcgggtgccg ccagccccgg ctcctgtgca ctgcgcttgt cggccagcac
     6421 gaacaccagg atcgcgacca gcgcagcaat gatcgcgtag tcgacccagc gccagtccca
     6481 gcgaaacgcc ggcgcgtcct cttccccggt tgccccggtg cgcaccacgc cttccggggt
     6541 caagtcgtag acccaggcca tgatcatcgc catgggaaac acgagcgcca gcaccagcgc
     6601 gatcacgcgc aaggagcctt ccggcccgcc gatgatgtcc agcagcacgt cggcgacctg
     6661 caggatcaac cagctgccga cgaggtagac gcccgcgagc cggaacacgt ggcggcgacg
     6721 cagctccctc agtacccgat tgaccgcgga cgctcgcccc gacaccgccc actccatgcg
     6781 cgcagcgcag caaacctgtc gcccttccgg cgcgctggag aaagtatagg cacaaacccg
     6841 agcgtttagc ccgcgctgat cggcggcccg gcgctcgacg cgccccaggt cgctactgcg
     6901 ggtcgccgac gctgccgaac tgctcgcgga aaaacatcac cgtggcggcc tggaagtagt
     6961 cgcggttcgg cttcttggcg aaaccgtggc cctcgtccat ggccagcagg taccacacct
     7021 cgccgccgtt gccgcgcact tcgcgcacga tctgctccga ttccgtgtag ggcacccgcg
     7081 gatcgttgaa gccctgggca acgaacagcg gcgaactgat ctgctcggcg ttgttcagcg
     7141 gcgagatacc ttccaggaat tcgcgcatcg ccgggtcccg ctcgtcgccg tactcgacgc
     7201 gtcgcaggtc gcggcgatag gcctcggtgt tctccaggaa ggtcacgaaa ttgctgatgc
     7261 cgacgatgct cacgccaccc agcaggcggt cgtcgtagtg caccagcgag gccagcacca
     7321 tgtagccgcc gtaggagccg ccgtagacaa tgacgcgctc ctcgtccagg tcgggctgcg
     7381 tggcgatcca gtcgagcagc gcgccgatgt cccgcaccga gtcctcgcgc agcacgccgt
     7441 tgtccagctg caggtagctc ttgccgtaac cggccgagcc gcgcacgttc gggctgacga
     7501 ccgccgcgcc gagctcgttc acccagtagg cgaacaacga attgaaggcc gggcgggact
     7561 gtgcctccgg cccgccgtgg atctgcacga tcaccggccg cggccccgcc tggaccgggc
     7621 gatagaccca ggcggggacc tcgagaccgt cgaagctggt gaaggtcacg agctccggca
     7681 ccgggaaacg cgccgtgtcc aggccgccga cctcgctctc ggtccagcgc accaggtccc
     7741 gcttgcgcag cccatagacg tacacgtcgc ttggcgattg cggcgtgttc agcaccatgg
     7801 ccagggcgcg gccgtcgggg ctgaaaccaa gcccgccgat cagcccgacc ggcagctccg
     7861 gggcgggcag cgcgcgcttg cgccgcagat ccagcaggtg caactcggcc ataccgccgg
     7921 cattcaccac gaaggccatc gtttcccggt cacgcgacaa ggtgaacatg ttcacgtccc
     7981 acgggatgtg cgccgacaag ggcctactgt tgccgctcgc caggtcgaaa tggtgcagct
     8041 gcttgaactc ggtgccgtgg tcgtgcacca ggtagacgcc cttgccggtc cggtcgaagg
     8101 cgccggctgc gtagccgacc ggtccgtcct gcggattgac ctgctgcacc gccccgctct
     8161 ccaggtccac gacgtgcacc tcggcgtcgg tgatggaacg gtaattgatg acgatcagcc
     8221 gcgagtcgtc gggcgcccag tccatcggca cccaaagacc gctgccctcc agcgccagca
     8281 ggtgcgtttc atgtccgccc gcgggcgtag cgacatagat gtcgtagttg cggccgtcgc
     8341 ggcgcgtgct ggcataagcg aaacggtcgc cgcggttgga ccacaccggc ccggtgttac
     8401 gcgagcggcc gtcggtcaac agccggctct cgctggtgtc gagatcgagc cagtaaagct
     8461 ggaagaactc gttgccgccg atatcccgcg cgtagatgaa gccccgcaat gccgggtcgg
     8521 gtgacaccga cacggtgttg atcggctcct tgaaaaaggt cagctgctgg cgggccccca
     8581 gcggctggcg cacgcggtgg acctggttgg tgtcgccaaa acgcgtcgag atcagcatgc
     8641 tgccgtcgtg cagccagctg ccgaagccgg cggcgcgcgt gttctggtac tgctccagcc
     8701 gctcggtgat ctccgccggg atctccggca ggttctcgag caccaggttg ccacgttcca
     8761 cccgctcggc ggatgcatgc agcggggacg acaacgcgag cgacagggcg acaggcaata
     8821 gcattctgat cagcacgtga ggcctccagg tccggctttg cggggtggtt ggtgcccggg
     8881 gccggagtcg aaccggcaag gccttgcggc cgggggattt taagtcccct gcgtctacca
     8941 gtttcgccac ccgggcgacg ctgcgggcaa cggtatcaca ggggaatgtt ggaggcgcgg
     9001 accggaatcg aaccggtgtt cacgg
//
LOCUS       DAOMPG010000085         8981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3332_length_8981_cov_3.782545, whole genome shotgun sequence.
ACCESSION   DAOMPG010000085 DAOMPG010000000
VERSION     DAOMPG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_8981_cov_3.782545"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLS34_06210"
     CDS             <1..377
                     /locus_tag="PLS34_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87095.1"
                     /translation="RAARAGGAGFFGLVSAAGAHPRSRNFYLRTKGEAEAAVEALGFS
                     SLAIMQPGLLRGEREEFRAGERLGQAAAPLLDRVLLGPLARYRSVVIDAVAAALEVAA
                     RRRAPGVHRYDSPAIEALAREP"
     gene            367..789
                     /locus_tag="PLS34_06215"
     CDS             367..789
                     /locus_tag="PLS34_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRP87096.1"
                     /translation="MSLESPPAAPGLLRRLAACIYDGLVLAAVLMVAGALWVAVSRTA
                     AAPGDWLFRGYLLGVSATFFGAFWTRGETLGMRAWKLRLVAADGQPPGWGRALLRFAT
                     ALISWAALGLGFLWVLVDRDRLAWHDRLSGTRLVMNRG"
     gene            complement(801..1865)
                     /gene="lptG"
                     /locus_tag="PLS34_06220"
     CDS             complement(801..1865)
                     /gene="lptG"
                     /locus_tag="PLS34_06220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRP87097.1"
                     /translation="MTLLDRYLAAAAARATLLVMVVLLALIGFISLTTQLQDVGRGSF
                     QAADAILVVIAQWPRNAFDIFPIAALLGGMMGLGALARHSELVAVRAAGVGLRRLGFS
                     MLIAGGLFAAAAAALGEWVMPPAERFADEYADELKRERSSLSGLSGSWLRDGDRVFNI
                     LQVESLERLGGVYVYRFDDAGRLDSVALARRAEVGHDRQWTLEDVELTRFGERIETET
                     LPTLELATGIDPAVMSVSVIDTNSMSIPMLREYIRYLEANDLQAGRYEMAMWQRIANV
                     AAVPAMLLLALPFVFGPLRSGGNSARFVVGFLIGILYILGKGALADGAQVYGAPIWLS
                     AWAPVGVILAGAFIGLSRVR"
     gene            complement(1862..2956)
                     /gene="lptF"
                     /locus_tag="PLS34_06225"
     CDS             complement(1862..2956)
                     /gene="lptF"
                     /locus_tag="PLS34_06225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRP87098.1"
                     /translation="MSGIIDRYLLREIFRSFVGVTVVLMLILLGDQFARGLARAAADR
                     IPREAVLKLMGLTSIEYLSLMVPAAYFFAVMLTLGRLYRDSEMAALNACGVGAGRLLR
                     PLLWSAALVASGLAVLSFDTVPWAARQSLEIRQEGQRQVQITALEPGRFRSASGGRLV
                     FYARDRNDAGQLTGVFIQQRVEQQVEVVTAARAEQRVSLERDENYLVLYDGIRYSGRP
                     GSPEFRTARFQEHGIPLPVPGRADPAQQRRQMSTFDLLGTGLAEDAAELHWRLSAPAS
                     LLVLTLLAVPLGRAPPRSGRYGRLGFAILVFVLYSNLLGFGRMLIENGTVPQWLGLWW
                     IHGLLLLVGFAMLYLQGGGAWRLRARWRAA"
     gene            3064..4560
                     /locus_tag="PLS34_06230"
     CDS             3064..4560
                     /locus_tag="PLS34_06230"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRP87099.1"
                     /translation="MDFVAKTGSATALRTDCAIVGVYRNGRLSQAAREIDAVAGNLVA
                     GLVKRGDFIGKPGATLLLHHVPETSAKRLLLVGLGEPGKLDMKGWRKAHGAAVGALLG
                     TGAKDAFSYLSTEEVADSDTYLLARHAAEAAEEAAYRFDELKSQLDAPAPRLAKLGLW
                     VPDRKSQSAADLGFRHGKAVARGQSLARRLGNLPGNVCTPTYLAEQAGKLGAEHAGLK
                     VSVLEQAQMKKLGMNALLSVAQGSRQPPKLIIMEWHGGPKQKAPVALVGKGITFDTGG
                     VSLKPPPAMDEMKFDMCGAASVIGTMATIIALELPINVVGLVPAAENMPDGLASRPGD
                     IVTTMSGQTVEILNTDAEGRLILCDALTYARRFKPSTVIDVATLTGACVIALGHHLSA
                     VMSSDDSLAREILRAGVDAEDRAWHMPIGEDYDEELKSNFADFANVAGREGGAITAAC
                     FLARFTRDLKWAHLDIAGTAWKTGLAKGATGRPVPLLAQFLMSRAGVA"
     gene            4580..5029
                     /locus_tag="PLS34_06235"
     CDS             4580..5029
                     /locus_tag="PLS34_06235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRP87100.1"
                     /translation="MTRVDFYVLEDSAEQARERFACRLTEKAWRLKHSVYLFAASPAE
                     AQRLDGLLWTFSDRSFLPHVLDAPDLDPALAAASPVRVGAGEPTFEAELLVNLGAEVP
                     LFFSRFERVAEIVGGNDAQRTLARERFRFYRDRGYALETHHIGRRAA"
     gene            5026..5334
                     /locus_tag="PLS34_06240"
     CDS             5026..5334
                     /locus_tag="PLS34_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87101.1"
                     /translation="MSDKPENPPPVVPVLVKQAAEPTGDGPPVLQSIAIPAPRRLSDH
                     ELAALKAELVAVAWEVVVDLLHSAVREVEAALFEQVSSRLRDELPDLVERALRNHLRT
                     "
     gene            5353..8118
                     /locus_tag="PLS34_06245"
     CDS             5353..8118
                     /locus_tag="PLS34_06245"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRP87102.1"
                     /translation="MQKTYSPEAIEAAIYADWERLGCFAPAGSGDPYCIMLPPPNVTG
                     TLHMGHAFQDTIMDALTRLHRMRGRNTLWQPGTDHAGIATQMVVERQLNAAGENRRDL
                     GREEFVRRVWAWKQASGDTIERQLKRLGASVDWSRHKFTMDPELSRAVTEAFVRLHEE
                     GLIYRGKRLVNWDPVLHTALSDLEVVTEEELGKLWHFRYPLADGDGELVVATTRPETM
                     LGDTAVAVHPDDPRYAAFIGREIALPLTGRRIPVIADDYVDPEFGSGCVKITPAHDFN
                     DYEIGRRHGLEMINVLDDDGSLNDAVPAAYRGLDRFVARERVVADMDAQGLLLRVEDN
                     RMFVPRGDRSGAVLEPYLTDQWYVKIAPLAAPAIAAVEDGRVRFVPDNWSKTYFEWMR
                     NIQDWCISRQLWWGHRIPAWYDEQGGAHVGLDEADARRRSGLAADVPLRQDEDVLDTW
                     FSSALWPFSTLGWPDRTPALATWYPGTVLVTGFDIIFFWVARMIMMGLKFMGDVPFRE
                     VYIHGLVRDGEGQKMSKSKGNVLDPLDLIDGVDLDTLVEKRTTGMMQPQLKATVEKAT
                     RKEFPQGIPAFGTDALRFTFASLATTGRDVRLDLGRVEGYRNFCNKLWNATRFVLMNT
                     EGEDCGPAGELELGLADRWIRSRLGEATRVALESFDAYRLDLAAQAIYEFTWHEFCDW
                     YLELVKPVLNSDEASAAEQRGTRRTLAATLEALLRLLHPLMPFITEALWRRAAPLAAV
                     SGESIMQQPYPEAGSWPRDTQAETEMAWVQAFVLGLRQVRGEMNIPPGKPLAVLLQDA
                     GPADLECLAANRALLLRLARLESVEVLAAGAKAPPAATVLLGDMKLLVPMAGLIDAAA
                     EIARLAKQRDKLESDLAKTRAKLDKPSFVDNAPAEVVDKERRRADELGSAIANLEQQL
                     ERIRALA"
     gene            8183..>8981
                     /locus_tag="PLS34_06250"
     CDS             8183..>8981
                     /locus_tag="PLS34_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87103.1"
                     /translation="MLDGVILGKSRQLRLCLTCLLAGGHLLIEDVPGVGKTTLAHGIA
                     RALGLTYQRIQFTSDLLPADIVGVSIYERDSGAFRFHPGPVFAQLLLADEVNRATPKA
                     QSALLEAMEERQVTVDGVSHPLPQPFFVVATQNPADQVGTFPLPESQLDRFLMRIRLG
                     YPERAMERELLRGEDRRELLARLPSAAEAGALLRLRGEVSRVHASEALLDYVQALVAW
                     SRERPGFGAGLSPRAAIALLRSARAWALLDGRRAVIPEDVQAVTPPVV"
BASE COUNT         1347 a         3207 c         3170 g         1257 t
ORIGIN      
        1 cccgcgccgc gcgcgccggc ggcgccggct tcttcggcct ggtgtcggcg gcaggtgcac
       61 acccgcgctc gcgcaacttc tacctgcgca ccaagggcga ggccgaggct gcggtcgagg
      121 cgctcggctt ttccagcctg gccatcatgc agccggggct gctgcgcggc gagcgcgagg
      181 aattccgcgc cggcgaacgg ctgggccagg cggccgcgcc gctgctggac cgcgtgctgc
      241 tcggcccgct ggcacgttac cgcagcgtcg tgatcgatgc ggtggcggcc gcgctcgagg
      301 tcgccgcgcg ccggcgcgcg cccggggtac atcgctacga ttcgccggcc atcgaggcgc
      361 tcgcccgtga gccttgaatc gccccctgcc gcccccggcc tgctgcgccg gctggcggcc
      421 tgcatttacg acggcctggt gctggcggcg gtgctgatgg tggccggcgc cctgtgggtg
      481 gccgtcagcc gcaccgcggc ggcccccggc gactggctgt tccgcggcta cctgctggga
      541 gtgtcggcga ccttcttcgg cgccttctgg acgcgcggcg agacgctcgg catgcgtgcc
      601 tggaagctgc ggctggtggc ggccgacggg cagccgcccg gctggggccg cgccctgctg
      661 cgctttgcga cggcgctcat ttcctgggcg gcgctcggcc tcggcttcct gtgggtgctg
      721 gtggaccgcg accggctggc ctggcacgac cggctgtcgg gcacccggct ggtgatgaat
      781 cgcggctgag atggcgcccg tcagcgcacg cggctgaggc cgatgaaggc gccggcaagg
      841 atcacgccga ccggcgccca ggcgctgagc cagatgggcg cgccgtagac ctgcgcgccg
      901 tcggcgagcg cacccttgcc gaggatgtac aggatgccga tcaggaaccc gaccacgaag
      961 cgcgcgctgt tgccgcccga gcgcagcggg ccgaacacaa agggcaaggc cagcagcagc
     1021 atcgccggca ccgccgcgac gttggcgatg cgctgccaca tggccatctc gtagcgcccc
     1081 gcctgcaggt cattggcctc gagataccgg atgtactcgc gcagcatggg gatgctcatg
     1141 ctgttggtgt cgatcaccga gaccgacatc accgcggggt cgatgccggt ggccagctcg
     1201 agcgtcggca gggtctcggt ctcgatgcgc tcgccgaagc gcgtgagctc gacgtcttcc
     1261 agcgtccact gccggtcgtg gccgacctcc gcgcgtcgcg ccagcgccac cgagtcgagc
     1321 cgcccggcgt cgtcgaaacg atacacgtac acgccgccga gccgctcgag gctctcgacc
     1381 tgcaggatgt tgaacacgcg gtcgccgtcc ctgagccagc tgccggacag gcccgacagg
     1441 ctgctgcgct cgcgcttcag ttcgtcggcg tactcgtcgg cgaagcgctc ggccggcggc
     1501 atgacccatt cgccgagcgc ggccgccgcg gcggcgaaca gcccgccggc aatcagcatc
     1561 gagaaaccca gccggcgcag gccgacgccg gcggcgcgca ccgccaccag ctcgctgtgc
     1621 cgcgcgagcg cgccgagtcc catcatgccg cccagcagcg cggcgatcgg gaagatgtcg
     1681 aaggcgttgc gtggccactg ggcgatcacc acgaggatgg cgtcggcggc ctggaagctg
     1741 ccgcggccga cgtcctgcaa ctgcgtggtg aggctgatga agccgatcag cgccagcagc
     1801 accaccatca ccagcagcgt ggcgcgcgcg gccgcggccg ccaggtagcg atcgagcagc
     1861 gtcatgcggc cctccagcgc gcgcgcagcc gccaggcgcc gccgccctgc aggtacagca
     1921 tggcgaagcc gaccagcagc agcaggccgt ggatccacca caggccgagc cactgcggca
     1981 ccgtgccgtt ctcgatgagc atgcgtccga agcccagcag gttgctgtac agcacgaaca
     2041 ccaggatggc gaagccgagc cggccgtagc gcccggagcg tggcggcgca cggcccagcg
     2101 gcacggccag cagcgtcagc accagcagcg aagcgggcgc cgacagtcgc cagtgcagct
     2161 ccgccgcatc ctccgccagc ccggtgccga gcaggtcgaa ggtggacatc tggcggcgtt
     2221 gctgcgccgg gtcggcgcgg cccgggacgg gcagcgggat gccgtgctcc tggaagcggg
     2281 cggtacggaa ttccgggctg ccgggccggc ccgagtaacg gatgccgtcg tacagcacca
     2341 ggtagttttc gtcgcgctcc agcgagacgc gctgctcggc gcgcgccgcg gtgacgacct
     2401 cgacctgctg ctccacgcgc tgctggatga acacgccggt caactggccc gcgtcgttgc
     2461 gatcgcgcgc gtagaacacc agccggccgc ccgaggcgct gcggaagcgg cccggctcga
     2521 gggcggtgat ctgcacctgg cgctggccct cctggcggat ctccagtgac tggcgcgcgg
     2581 cccacggcac ggtatcgaag gacagcaccg ccaggccgct ggcgaccagc gcggccgacc
     2641 acagcagcgg ccgcagcagg cgcccggcgc ccaccccgca ggcgttgagc gccgccatct
     2701 cgctgtcgcg gtacagccgg cccagggtca gcatcaccgc gaagaagtag gccgccggca
     2761 ccatcaggct gaggtactcg atgctggtca ggcccatcag cttgagcacg gcctcgcgcg
     2821 ggatgcggtc ggccgccgcg cgggccagcc cgcgggcgaa ctggtcgccg agcaggatga
     2881 gcatcagcac cacggtgacg cccacgaagc tgcggaagat ctcgcgcaag agatagcggt
     2941 ctatgattcc cgacacggcg cccgcagttt cggcctgccc ccttatccat taaacttccg
     3001 catcttaccg cgtccgcgca gccccagggg ccggccggcg gtccataagg agggggagtc
     3061 gagatggatt ttgttgcaaa gacggggtcg gcgacggcgc tcaggactga ctgcgcgatc
     3121 gtcggcgtct acaggaacgg ccggctctcg caggccgccc gggagatcga cgccgtggcc
     3181 ggcaacctgg tggccggcct ggtcaagcgc ggggatttca tcggcaagcc gggggccacc
     3241 ctgctgctgc accatgtccc ggaaacttcg gcaaagcgcc tgctgctggt cggcctcggc
     3301 gagcccggca agctggacat gaagggctgg cgcaaggcgc acggtgcggc cgtcggggcg
     3361 ctgctcggca ccggcgcgaa ggacgccttc agctatctct ccaccgagga ggtggcagac
     3421 tccgacacct acctgctggc gcgtcacgcg gccgaggccg ccgaggaggc cgcctaccgc
     3481 ttcgacgaat tgaagtccca gctcgatgcg ccggcgccgc ggctggcgaa gctgggcctg
     3541 tgggtcccgg accgcaagtc gcagtcggcc gccgacctcg gcttccggca cggcaaggcg
     3601 gtggccaggg gccagtcgct ggcgcgccgg ctcggcaacc tgcccggcaa cgtgtgcacg
     3661 ccgacttatc tcgccgagca ggcgggcaag ctgggcgcgg agcacgccgg gctcaaggtc
     3721 agcgtgctgg agcaggcgca gatgaagaag ctgggcatga acgccctgct gtcggtcgcg
     3781 cagggcagcc gccagccgcc gaagctgatc atcatggaat ggcacggcgg gccgaagcag
     3841 aaggcgccgg tggcgctggt cggcaagggc atcaccttcg acaccggcgg cgtgtcactc
     3901 aagccgccgc cggccatgga cgagatgaaa ttcgacatgt gcggcgccgc cagcgtgatc
     3961 ggcaccatgg cgaccattat cgcgctggag ctgccgatca acgtggtcgg actagtgccg
     4021 gccgccgaga acatgcccga cgggctcgcc agccggccgg gcgacatcgt caccacgatg
     4081 tcgggccaga cggtggagat cctcaacacc gacgccgagg gccgcctgat cctgtgcgat
     4141 gcgctcacct atgcgcgccg cttcaagccg tccaccgtca tcgacgtcgc cacgctcacc
     4201 ggcgcctgcg tgatcgcgct cggccatcac ctgagcgcgg tgatgagcag cgacgattcg
     4261 ctggcccgcg agatcctgcg cgccggcgtc gacgcggagg accgcgcctg gcacatgccg
     4321 atcggcgagg actacgacga ggaactgaag agcaacttcg ccgatttcgc caatgtcgcg
     4381 ggccgcgagg gcggcgccat cacggccgcc tgcttcctgg cgcgcttcac ccgcgacctg
     4441 aaatgggctc acctggatat tgccggtacc gcctggaaga ccggcctggc gaagggcgcc
     4501 acgggccggc cggtgccgct gctggcccag ttcctgatga gccgggccgg cgtcgcctga
     4561 gcgccgcgcc tggaaaggga tgacgcgcgt tgacttctat gtcctcgagg attctgccga
     4621 acaggcacgg gaacgcttcg cctgccggtt gaccgagaag gcctggcggc tcaagcacag
     4681 cgtgtacctg ttcgcggctt caccggccga ggcgcagcgg ctcgacggac tgctgtggac
     4741 cttcagcgat cgcagcttcc tgccgcacgt gctggatgcg cccgacctcg acccggcgct
     4801 cgcggccgcc tcgccggtgc gcgtcggtgc cggcgagccg accttcgagg ccgagctgct
     4861 ggtgaacctc ggcgcggagg tgccgctgtt cttcagccgc ttcgagcggg tcgcggagat
     4921 cgtcggcggc aacgatgccc agcgcacgct cgcgcgcgag cgtttccgct tctaccgcga
     4981 tcgcggttac gccctggaaa cccaccacat cgggaggcgc gcggcatgag cgacaagccc
     5041 gagaacccgc caccggtcgt cccggtgctg gtcaagcagg ccgccgagcc gaccggcgac
     5101 ggtccgccgg tgctgcagtc gatcgccatc cccgcgccgc gcaggctgtc cgaccatgag
     5161 ctcgccgcgc tcaaggcgga gctggtggcg gtggcctggg aagtcgtcgt cgacctgctg
     5221 cactccgcgg tgcgcgaggt cgaggcggcg ctgttcgagc aagtctcgtc gcggctgcgg
     5281 gacgaactcc cggatctcgt tgaacgcgcc ctgagaaatc acctgcgcac ctgaacagga
     5341 ccgcaccgac ccatgcagaa gacctacagc cccgaggcca tcgaggcggc catctacgcc
     5401 gactgggaac gcctgggatg cttcgcgccc gccggcagcg gcgaccccta ttgcatcatg
     5461 ctgccgccgc ccaacgtcac cggcacgctg cacatgggcc acgccttcca ggacaccatc
     5521 atggatgcgc tgacccggct gcatcgcatg cgcggccgca acaccctgtg gcagccgggc
     5581 acggaccacg ccggcatcgc cacgcagatg gtggtcgagc gccagctcaa cgccgccggg
     5641 gagaaccggc gcgatctcgg ccgcgaggaa ttcgtccgcc gcgtgtgggc gtggaaacag
     5701 gcctcgggcg acaccatcga gcggcagctg aaacggctcg gcgcctcggt ggactggtcg
     5761 cgccacaagt tcaccatgga cccggagctg tcgcgcgccg tcaccgaggc cttcgtcagg
     5821 ctgcacgagg aaggcctgat ctatcgcggc aagcggctgg tcaactggga cccggtgctg
     5881 cacaccgcgc tgtccgacct cgaggtggtc accgaggaag aactcggcaa gctgtggcac
     5941 ttccgctacc cgctggccga cggcgacggc gaactggtgg tcgccaccac gcggcccgag
     6001 accatgctgg gcgacaccgc cgtggcggtg catcccgacg atccgcgcta cgcggcgttc
     6061 atcggccgcg agatcgccct gccgctgacc ggccggcgca tcccggtcat cgccgacgac
     6121 tacgtggatc cggagttcgg cagcggctgc gtcaagatca cgccggcgca cgatttcaac
     6181 gactacgaga tcggccgccg tcacgggctc gagatgatca acgtgctgga cgacgacggc
     6241 tcgctcaacg atgcggtgcc cgcggcctat cgcggcctcg accgcttcgt ggcgcgcgag
     6301 cgcgtcgtcg ccgacatgga tgcacagggc ctgctgctgc gcgtcgagga caaccgcatg
     6361 ttcgtgccgc gcggcgaccg cagcggcgcg gtgctcgagc cctatctcac cgaccagtgg
     6421 tacgtgaaga tcgcgccgct ggcggcgccc gccatcgccg cagtcgagga cggccgcgtg
     6481 cgtttcgtgc ccgacaactg gtcgaagacc tatttcgagt ggatgcgcaa catccaggac
     6541 tggtgcatca gccgccagct ctggtggggg catcgcatcc cggcctggta cgacgagcaa
     6601 ggcggcgcac acgtcggcct cgacgaggcg gacgcgcggc gccgctccgg gctggcggcc
     6661 gacgtgccgc tgcgccagga cgaggatgtg ctggacacct ggttctcctc ggcgctgtgg
     6721 cccttcagca ccctcggctg gccggaccgg acgccggcgc tggccacctg gtacccgggc
     6781 acggtgctgg tcaccggctt cgacatcatc ttcttctggg tcgcccggat gatcatgatg
     6841 ggcctgaagt tcatgggcga cgtgccgttc cgcgaggtct acatccacgg cctggtgcgc
     6901 gacggcgaag gccagaagat gtccaagtcc aagggcaacg tgctcgaccc gctggacctc
     6961 atcgacggcg tggacctcga cacgctggtc gagaagcgca ccaccggcat gatgcagccg
     7021 cagctcaagg cgacggtgga gaaagccacc cgcaaggagt tcccgcaggg tatccccgcc
     7081 ttcggcaccg acgcgctgcg cttcaccttc gcctcgctgg ccaccaccgg gcgcgacgtg
     7141 cgcctcgacc tggggcgcgt cgagggctac cgcaatttct gcaacaagct gtggaacgcc
     7201 actcgcttcg tgctgatgaa caccgagggc gaggactgcg gccctgcggg cgagctcgag
     7261 ctcggcctcg ccgaccgctg gatccgctcg cgtctcggcg aggcgacgcg tgtcgcgctg
     7321 gaatccttcg acgcctaccg gctggacctc gccgcgcagg ccatctacga gttcacctgg
     7381 cacgagttct gcgactggta cctggagctg gtgaagccgg tgctcaactc cgacgaggcg
     7441 agcgcggccg agcagcgcgg cacgcgccgc acgctggcgg cgacgctcga ggcgctgctg
     7501 cggctgctgc atcccctgat gccgttcatc accgaggcgc tgtggcgccg tgccgcaccg
     7561 ctcgccgccg tctcgggcga gtccatcatg cagcagccgt atccggaggc cggcagctgg
     7621 ccacgcgata cgcaggccga gaccgagatg gcctgggtgc aggcgttcgt gctgggcctg
     7681 cgccaggtgc gcggcgagat gaacatcccg cccggcaagc cgctggcggt gctgctgcag
     7741 gacgccggcc cggccgacct cgagtgcctg gcggccaacc gtgcgctgct gctgcgcctg
     7801 gcgcggctcg agtcggtgga ggtgctggcc gccggcgcca aggcgccgcc ggccgccacg
     7861 gtgctgctcg gcgacatgaa gctgctggtg ccgatggccg ggctgatcga cgccgccgcc
     7921 gagatcgcgc ggctggcaaa gcagcgcgac aagctcgaat cggatctcgc caagacccgc
     7981 gccaagctgg acaagccctc ctttgtcgac aacgccccgg ccgaagtcgt cgacaaggaa
     8041 cggcgccgcg ccgacgagct gggtagcgcg atcgccaacc tcgagcagca gctggagcgc
     8101 atccgtgccc tcgcctgagc agaccgcggc ccgcgatctg caggcggccg acgatctggc
     8161 gcagctcggc cgtgcgctgc agatgctcga cggcgtcatc ctcggcaagt cgcgccagct
     8221 gcgcttgtgc ctgacctgcc tgctcgccgg cggccacctg ctgatcgagg acgtaccggg
     8281 cgtcggcaag accacgctgg cgcacggcat cgcgcgcgcg ctgggcctca cctaccagcg
     8341 tatccagttc accagcgacc tgctgccggc cgacatcgtc ggcgtgtcca tctacgagcg
     8401 cgacagcggc gccttccgtt ttcatcccgg gccggtgttc gcccagctgc tgctcgccga
     8461 cgaggtcaat cgcgccacgc cgaaggcgca gagcgcgctg ctcgaagcca tggaggaacg
     8521 ccaggtgacg gtggacggcg tcagccatcc cctgccgcag ccgttcttcg tggtggcgac
     8581 ccagaacccg gcagaccagg tcggcacctt ccccttgccg gagtcgcagc tcgatcgctt
     8641 cctgatgcgc atccgcctcg gttacccgga acgggccatg gagcgcgagc tgttgcgcgg
     8701 cgaggaccgg cgcgagctgc tggcgcgcct gccgtccgcc gccgaggccg gcgcgctgct
     8761 gcgcctgcgc ggtgaagtga gccgcgtgca cgcctccgag gcgctgctcg actacgtgca
     8821 ggcgctggtg gcatggagcc gggagcggcc cggcttcggc gccggcctgt cgccgcgcgc
     8881 cgccatcgcc ctgctgcgca gtgcgcgggc ctgggcgctg ctcgacggcc gccgcgcggt
     8941 gattccagaa gacgtgcaag ccgtcacgcc gccggtggtg g
//
LOCUS       DAOMPG010000086         8958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3341_length_8958_cov_3.731214, whole genome shotgun sequence.
ACCESSION   DAOMPG010000086 DAOMPG010000000
VERSION     DAOMPG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_8958_cov_3.731214"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLS34_06255"
     CDS             complement(<1..508)
                     /locus_tag="PLS34_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRP87104.1"
                     /translation="MSTPLTIAIDAMGGDSAPAVPVRATLELLRRQPALHAVLVGQSE
                     VIEPLIEHRSTGLRARVSVQPATEIVGMDEPPAEALRRKRDSSMRVAINLVKEGRAAA
                     CVSAGNTGALMATAKFVLKTLPGVDRPAILSGIPALGGQTLMLDLGANAVCTARQLCE
                     FAVMGDIVA"
     gene            complement(564..755)
                     /gene="rpmF"
                     /locus_tag="PLS34_06260"
     CDS             complement(564..755)
                     /gene="rpmF"
                     /locus_tag="PLS34_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRP87105.1"
                     /translation="MAVQKSRKTPSKRGMRRSHDALGKPTLSVDPTSGETHRRHHVTA
                     DGFYRGKKVIDVATVVDEE"
     gene            complement(775..1308)
                     /locus_tag="PLS34_06265"
     CDS             complement(775..1308)
                     /locus_tag="PLS34_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRP87106.1"
                     /translation="MSDELDRPLEPRALAARDRRLRSETPLLDWTRLAALDPSGAVPD
                     TVVRVDAGFREDENGVALLEGQVVARLRCHCQRCLEEMELEVCARPKLMFGRADELGA
                     AAVAAGFEHCELEPGMTLRELLEDEVLLAVPAFPVHERSEDCGKLAAKLAELEPGEGG
                     ERSSSPFAVLAALKRRN"
     gene            1405..2016
                     /locus_tag="PLS34_06270"
     CDS             1405..2016
                     /locus_tag="PLS34_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HRP87107.1"
                     /translation="MSQATAADTPRPTLVLASASRYRRALLERLGLPFIQDAPAVDES
                     LRTGEAPAEAAMRLARAKARAVLERHPAAIVIGSDQVAVRGEEILGKPGDAQRAAQQL
                     TLSAGRHVEFLTAVCVLSGARPDEPEEHIDITRVVFRPLTPAEIRNYVARDAPEDCAG
                     SFRAEGLGIALFERIDSQDPTGLIGLPLIWLAQALRRLDLPVL"
     gene            complement(2032..2682)
                     /locus_tag="PLS34_06275"
     CDS             complement(2032..2682)
                     /locus_tag="PLS34_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRP87108.1"
                     /translation="MAKPTRLVVFDWDGTLMDSTAQIVAAASGAIAQLGLPARPPEAI
                     RDIIGLGLRESWQRLFPELGPQSFTPFVAAYRDHFFAPELQTAQLFARAEEVVAELAG
                     RGFLLGVATGKSRRGLDRDLETTGLGRFMAGSRTADETRSKPHPDMLLELMAALGTPA
                     AATLMVGDTEWDLEMARRAGVPAVAVSYGAHAPERLRAHAPIALIDALDALLPLLD"
     gene            complement(2675..3637)
                     /gene="rluC"
                     /locus_tag="PLS34_06280"
     CDS             complement(2675..3637)
                     /gene="rluC"
                     /locus_tag="PLS34_06280"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HRP87109.1"
                     /translation="MVSPSAGSRPSVELVRIGEDEAGQRLDNFLLARLKGVPKSHVYR
                     LLRKGEVRVNKGRAKPDYRLATGDQVRLPPVRRSEPGAVPLRGRNAGLKLEDRILHED
                     DRLIVLDKPTGIAVHGGSGLSHGVIEALRAARPDAPYLELVHRLDRETSGCLLIAKRR
                     SMLRALHALLREGRVEKRYLALVKGRWEMGQVRLEDRLRKTVRGGERMVTVDETGKAA
                     ASIFRPVEIGSAASLLEVRIMTGRTHQIRVQAAEAGHPLAGDERYGDREYNRRMKGLG
                     LERLFLHAASVGWEDPVSGEWRVFSAPLPDDLREVLSRLESHHG"
     gene            4062..>6370
                     /locus_tag="PLS34_06285"
     CDS             4062..>6370
                     /locus_tag="PLS34_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRP87110.1"
                     /translation="MKRMLVNATQREELRVAVVDGQKLYDLDIEIPSREQRKANIYKG
                     RITRLEQSLEAAFVEFGADRHGFLPLKEISPEYFRGGEQNGGRVNIRDVLREGQEIVV
                     QVEKDERGNKGAALTTYLSLAGRFLVLMPNNPKAGGVSRRIQGDDREAVREAMDDLEV
                     PEGMGAIVRTAGVGRNLEELQWDLDTLLQVWEAIKRTAVERPAPFLIYQESNAILRAL
                     RDHFSAEIGEILVDEAETYEEARLFMERVMPHNLRKLKYYADAVPLFTRFQIESQIES
                     AYSHVVELPSGGSIVIDHTEALLSIDINSARATKGDDIEMTALNTNLEAADEIARQLR
                     IRDLGGLIVIDFIDMGPQRNQREVENRLRDAVKMDRARIQIGRISRFGLLEMSRQRLR
                     PSLGESSHITCPRCQGWGAIRSVESLSLSILRLIGEEARKERSARIIVEVPVDVGTYL
                     LNEKRGWLGEIEQRDKVHIIIVPREHLESPNFTIRRVRDDAANLPENAGVSYQLPPAE
                     GEEPDDVTPGLKPARKVEQPAVSGIVPATPIPMRPDEAPAPAPAGPGFWARLKTWFAG
                     LGATRTPAAAVPREDQDRKKPDSGRRSRQRDDDRRKRGDRQRPESGKRSRGRGKDQDR
                     ERGRDGGRDKSAESGKEPSRDRQQDQDRSRDQDRQRDKAAAADKPKPTDGQQQGPAGD
                     GTADGETRSKRRRRRRSRKPRQDGDQAAVEQAGAAPPAESGGATQPGEGSETRTESRP
                     PVKAQPPGMPVAVAVAAQEPKPEPNVEPRP"
     assembly_gap    6371..6418
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6419..6598
                     /locus_tag="PLS34_06290"
     CDS             <6419..6598
                     /locus_tag="PLS34_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87111.1"
                     /translation="PAPAPEPKPEPKPEPKLTRIETVVENVQRSLPIEPPREPARPEP
                     KPPEPPTGNSPGNSQ"
     gene            complement(6602..7099)
                     /locus_tag="PLS34_06295"
     CDS             complement(6602..7099)
                     /locus_tag="PLS34_06295"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRP87112.1"
                     /translation="MATEEKREQVSVLFVCLGNICRSPTAEGVFRKLVADAGLHERIR
                     IDSAGTAGYHEGHPPDARAAAAAAARGFELDGIRARRVAVEDFELFDLILAMDEDNLL
                     DLRRAAPDHARAELRLLLEYAAGRSETAVPDPYYGGRNGFEQVLDLVTDACAGLLEEL
                     RRRLP"
     gene            7220..7504
                     /locus_tag="PLS34_06300"
     CDS             7220..7504
                     /locus_tag="PLS34_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87113.1"
                     /translation="MDNGITLERLQSLVGTELRYQGIRCMLVDVLEEPPVLVLRPLGA
                     EPVIQADNFGKPMRHAPQLFELPVFGPDGVSLSAELQLIRVPGGLPTAGD"
     gene            complement(7467..8240)
                     /gene="kdsB"
                     /locus_tag="PLS34_06305"
     CDS             complement(7467..8240)
                     /gene="kdsB"
                     /locus_tag="PLS34_06305"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRP87114.1"
                     /translation="MSGFRVVIPARFASTRLPGKPLLQIAGRAMIQWVWQRAMEAGAV
                     EVLVATDHAGIADACRAFGAAVCMTDAAHPSGTDRIAEVAARRGWDDAEVVVNVQGDE
                     PLLPPALIRQVAELLLRTDADVGTLGTPIRSLDDYLDTNVVKLAARQDGAALYFSRAP
                     IPWHRDGAAAGLASQQRFAGALRHIGLYSYRVGALRRLAAAPPCALEELERLEQLRAL
                     WLGMRIQVDVATEAPPAGIDTPADLARVNRLLSASPREP"
     gene            complement(8237..8434)
                     /locus_tag="PLS34_06310"
     CDS             complement(8237..8434)
                     /locus_tag="PLS34_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRP87115.1"
                     /translation="MDHRLLDILACPICKGPLVHRREEHELVCRGDRLAWPVRDGVPV
                     LLVAEAREVSQAELLAMEQRR"
     gene            complement(8438..>8958)
                     /gene="lpxK"
                     /locus_tag="PLS34_06315"
     CDS             complement(8438..>8958)
                     /gene="lpxK"
                     /locus_tag="PLS34_06315"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRP87116.1"
                     /translation="VEIVVVDGRRGFGNGRCLPAGPLREPPTRLATVDLVVCNGGGAC
                     PPGALTMRLAGEVAVRLDGGLRRPLAEFAPGPVHAVAGIGDPERFFAMLEGQGLQLVR
                     HPLADHASVPAELLAPADGRPVLMTSKDAARCSGRPAAASCWEVPVTPDFGADAARLA
                     AVLAQRLGLREA"
BASE COUNT         1418 a         3292 c         3008 g         1192 t
ORIGIN      
        1 aggcgacgat gtcgcccatc accgcgaact cgcacagctg gcgcgcggtg cagaccgcat
       61 tggcgccgag atccagcatc agcgtctggc cgcccagcgc cgggatgccc gacaggatgg
      121 cgggccggtc cacgcccggc agggtcttga ggacgaactt ggcggtggcc atcagcgcgc
      181 cggtgttacc cgcgctgacg cacgcggcgg cgcggccctc cttcaccagg ttgatggcga
      241 cgcgcatcga cgagtcgcgc ttgcgccgca atgcctccgc gggaggctcg tccatgccga
      301 cgatttcggt ggcgggctgg acgctgacgc gtgccctcag gccggtcgag cggtgctcga
      361 tcagcggctc gatgacctct gactggccga ccagcacggc gtgcaacgcc ggctgccggc
      421 gcagcaactc caacgtcgcc cggacaggga cagcgggcgc gctgtccccg cccatggcgt
      481 cgatcgcaat ggtcagcggt gtcgacatca ggccgaggcg acggtgcgga acgagacgcg
      541 cggcctggtc cggccggcga ccgctattcc tcgtcgacca ccgtggcgac gtcgatcacc
      601 ttcttgccgc gataaaagcc gtccgcggtg acgtgatggc gacgatgggt ctcgccgctg
      661 gtggggtcga ccgacagcgt cggcttgccc agcgcatcgt gcgaacgacg catgccgcgc
      721 ttcgaggggg tctttctgct cttctgtaca gccatggtgg cgttgctccg tcaatcagtt
      781 cctgcgcttg agtgcggcga gcaccgcgaa cgggctcgac gacctctctc cgccctcgcc
      841 gggctccagc tccgccagct tcgcagccag cttgccgcaa tcctcgctgc gctcgtgcac
      901 cgggaaagcc ggcaccgcga gcagcacttc gtcctcgagc aactcccgca gcgtcatgcc
      961 gggctccagc tcgcagtgct cgaagccggc ggcgaccgcc gctgctccaa gctcgtcggc
     1021 gcgcccgaac atcagcttcg gccgcgcgca cacctcgagt tccatctcct cgagacaacg
     1081 ctggcaatgg cagcgcagcc tcgcgacgac ctggccctcg agcagcgcca cgccgttctc
     1141 gtcctcccgg aagccggcat ccacccggac caccgtgtcc ggcaccgccc cggaggggtc
     1201 gagcgccgcg agccgcgtcc agtccaggag cggcgtctca gagcgcagcc gccggtcccg
     1261 cgcggccagc gcccggggct ccagggggcg gtcaagctcg tcggacataa gccgagtaga
     1321 atatcgagcg gcgcgaatcc tgtcaaagcg ttgaaagcct gaatgttttg cccgcgggtc
     1381 gccgcgcgcg ttagtctagc gcgcatgagc caagcaacgg cagctgacac gccgcgcccg
     1441 acactggtcc tggcctccgc ctcgcgatac cggcgcgccc tgctggagag gctcgggctg
     1501 cctttcatcc aggatgcgcc cgccgtcgac gagtccctgc gcacgggcga ggcgccggcc
     1561 gaggccgcca tgcgcctggc acgcgccaag gccagggccg tgctcgagcg ccacccggcc
     1621 gcgatcgtca tcggctcgga ccaggtggcg gtgcgcggcg aggagatcct gggcaaaccg
     1681 ggcgatgcgc aacgagccgc gcagcagctg acgctctcgg cgggccgcca cgtcgagttc
     1741 ctgaccgcgg tgtgcgtgct cagcggcgcg cgaccggacg agccggagga acacatcgac
     1801 atcacccgtg tcgtgttccg gccgctgacg ccggccgaga tccgcaacta cgtggcgcgg
     1861 gacgcccctg aggactgcgc cggcagcttt cgcgccgaag gactcggcat cgcgttgttc
     1921 gagcgcatcg acagccagga cccgaccggg ctgatcggcc tgccgctgat ctggctggcc
     1981 caggccctcc gtcggctcga cttgccggtg ctctgacccc gccgtcgccc ttcagtccag
     2041 cagcggcaac agcgcgtcga gcgcatcgat cagggcgatc ggcgcgtggg cgcgcaggcg
     2101 ctccggggca tgcgcgccgt agctcacggc cacggccggc acgccggcgc gccgcgccat
     2161 ctccaggtcc cactccgtgt cgcccaccat cagcgtcgcc gccgcgggcg tgcccagcgc
     2221 tgccatcagc tccagcagca tgtccggatg tggcttggag cgggtctcgt ccgcagtccg
     2281 ggagcccgcc atgaaccgcc cgaggccggt cgtctccagg tcgcggtcca gcccgcggcg
     2341 actcttgccg gtcgccacgc ccagcaggaa gccgcggccg gcaagctcgg ccacgacctc
     2401 ctcggcacgt gcgaagagct gcgccgtctg cagttcgggg gcgaagaagt ggtcgcggta
     2461 ggccgccacg aacggcgtga agctctgcgg cccgagctcg ggaaacaggc gctgccagga
     2521 ttccctcagg cccagcccga tgatgtcccg aatggcctcc ggcggccgtg ccggcaggcc
     2581 cagctgcgcg atcgcgccgg acgccgccgc gacgatctgc gcggtcgaat ccatcagcgt
     2641 gccgtcccag tcgaacacca ccagccgggt cggcttagcc atggtgcgac tccagccgcg
     2701 acagcacctc ccgcaggtcg tccggcaacg gcgccgagaa cacgcgccac tcgccgctca
     2761 ccggatcctc ccagccgacc gaggccgcgt gcaggaacag tctctccagc ccgaggccct
     2821 tcatccggcg gttgtactca cggtcaccgt agcgttcgtc gccggccagc ggatggcccg
     2881 cctcggcggc ctgcacgcga atctggtgcg tgcgcccggt catgatcctg acttccagca
     2941 gcgaagccgc cgaaccgatc tccaccgggc gaaaaatgct ggctgccgcc ttgccggtct
     3001 catccacggt caccatgcgc tcgccgccgc gcacggtctt ccgcagtcgg tcctcgagcc
     3061 gcacctggcc catctcccag cgacccttga ccagcgccag gtagcgtttc tccaccctgc
     3121 cctcgcgcag cagcgcatgc agggcgcgca acatgctccg ccgcttggcg atcagcaggc
     3181 acccgctggt ctcacggtcc agccggtgga cgagttcgag gtagggtgcg tcgggccgcg
     3241 ccgcccgcag cgcctcgatg acaccgtgcg acagcccgct gccgccgtgc acggcgatgc
     3301 ccgtcggctt gtcgagcacg atcagccggt cgtcctcgtg caggatccgg tcctccagct
     3361 tcagcccggc gttgcgcccg cgcagcggta cggcgcctgg ctccgagcgg cgcaccggcg
     3421 gcaggcgtac ctggtcgccc gtcgccagcc ggtagtccgg tttcgcacgg cccttgttga
     3481 cccgcacctc gcccttgcgc agcaagcgat agacatgcga cttgggcacg cccttgaggc
     3541 gcgcgagcag gaaattgtcg agtcgctggc ccgcctcgtc ctcgccgatg cggacgagtt
     3601 ccacggacgg ccggctcccg gcggatggtg aaaccaaatt ctcctcccgg attcaggggc
     3661 ttagcgattg ctgaggggtc gagcggttgc tatagtacgc cacgggccgc gtgccacgct
     3721 ccaattggaa ggctttccgg gcgtactcgc tgccgctggt tacgggccgg tcgcggagca
     3781 tccgctgacc ggcatgcatt tgcaatttct ttccatcgct cggccgacgc ccccgcctgc
     3841 ttccgcaaca cggttgcccg ggacagcgga gcgcaaaaac tggccgcggg cggtgcccgg
     3901 cacggacgga atgaccggca tcctgcactt ggtcctgatc gttttactgc cgaccctgca
     3961 cctcgggggc gctacgccgt gcccgaacct gtcccgcgtc cgcgagcgcg cccccgccat
     4021 cgggaggccc acggtcgacc tggatctgcg agttccaatt catgaaaaga atgctggtga
     4081 acgcgactca acgcgaagag ttgcgtgtgg ccgtcgtcga cggccagaag ctctacgacc
     4141 tcgacatcga gattccctcc cgggaacagc gtaaggcaaa catctacaag ggccgcatca
     4201 cgcgactcga gcagagcctc gaggccgcgt tcgtggagtt cggcgcggac cgccacggct
     4261 tcctgccgct gaaggaaatc tcgccggaat acttccgcgg cggcgagcag aacggcgggc
     4321 gcgtcaacat ccgcgacgtg ctgcgcgagg gccaggaaat cgtcgtccag gtggagaagg
     4381 acgagcgcgg caacaagggc gccgcgctga cgacctacct gagcctcgcg ggccgtttcc
     4441 tggtgctgat gcccaacaac cccaaggccg gcggcgtgtc gcgccgcatc cagggcgacg
     4501 accgcgaagc ggtgcgcgaa gccatggacg acctcgaggt gcccgagggc atgggcgcga
     4561 tcgtgcgcac cgcgggcgtg ggccgcaacc tcgaggagct gcagtgggac ctcgacaccc
     4621 tgctgcaggt ctgggaagcc atcaagcgga ccgcggtgga gcgccccgcg cccttcctga
     4681 tctaccagga aagcaacgcc atcctgcgcg ccctgcgcga tcatttctcg gcggagatcg
     4741 gcgagatcct cgtggacgag gcggaaacct acgaggaggc acgcctgttc atggaacggg
     4801 tgatgccgca caacctgcgc aagctcaagt actacgccga cgccgtgccg ctgttcaccc
     4861 gcttccagat cgagagccag atcgagagcg cctactcgca cgtcgtcgaa ctgccgtccg
     4921 gcggcagcat cgtcatcgac cataccgagg cgctgctctc catcgacatc aactcggcgc
     4981 gcgccaccaa gggcgacgac atcgagatga cggcgttgaa cacgaatctc gaggcggccg
     5041 acgagatcgc gcgccagctg cgcatccgtg acctgggcgg cctgatcgtc atcgacttca
     5101 tcgacatggg tccccagcgg aaccagcgcg aggtcgagaa ccggctgcgc gacgcggtga
     5161 agatggaccg tgcccgcatc cagatcgggc gtatctcgcg cttcggcctg ctggagatgt
     5221 cgcgccagcg cctgcggccg tcgctcggcg aatccagcca catcacctgc ccgaggtgcc
     5281 agggctgggg cgccatacgc agcgtggagt ccctgtcgct gtccattctc cgcctgatcg
     5341 gcgaggaggc gcgcaaggag cgcagcgcga ggatcatcgt ggaagtgccg gtcgacgtcg
     5401 gcacctacct gttgaacgag aaacgcggct ggctcggcga gatcgagcag cgcgacaagg
     5461 tgcacatcat catcgtgccc cgcgagcatc tcgagtcgcc gaatttcacc atccggcgcg
     5521 tgcgcgacga cgccgccaac ctgcccgaga acgccggtgt cagctaccag ttgccgcccg
     5581 ccgaaggcga ggagcccgac gatgtcacgc cgggactgaa gcccgcgcgc aaggtcgaac
     5641 agccggcggt gtccggcatc gtgcccgcca cgcccatccc gatgcgtccg gacgaggctc
     5701 ccgctccggc cccggccggg cccggcttct gggcccgcct gaagacctgg tttgccgggc
     5761 tgggcgcaac gcggaccccc gcggccgccg taccgcggga agaccaggat cgcaagaagc
     5821 ccgactccgg ccgcaggtcg cgccagcgcg acgacgatcg ccgcaagcgc ggcgaccgcc
     5881 agcggcccga gagcggcaag cgcagccgcg gacgcggcaa ggaccaggat cgggagcgcg
     5941 gccgggacgg cggccgtgac aagagtgccg agtccggcaa agaaccgtca cgggatcgac
     6001 agcaggacca ggaccggtcg cgcgaccagg atcgccagcg cgacaaggcc gctgccgccg
     6061 acaagccgaa gccgaccgac ggccagcagc agggtccggc gggcgacggc acggccgacg
     6121 gcgagacgcg cagcaagcgc cggcggcgcc ggcgcagtcg caagccccgc caggacggcg
     6181 accaggcggc cgttgagcag gcgggcgccg cgccgccggc cgagagcggc ggcgctaccc
     6241 agcccggcga ggggtcggag accaggacgg aatcgcgacc gccggtcaag gcccaacccc
     6301 ccggcatgcc cgtggccgtc gcggtggccg cacaggagcc gaagcccgag ccgaacgttg
     6361 agccgaggcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     6421 cgcgccggca cctgagccga agcctgagcc gaagcccgag ccgaagctga cccgcatcga
     6481 gaccgtggtg gagaacgtcc agcgcagcct gccgatcgag ccaccgcgcg agccggccag
     6541 gcccgagccg aagccgccgg agccgccgac cggcaacagc cccggcaaca gccagtaagc
     6601 gtcagggcag cctgcgccgc agctcctcca ggagcccggc gcaggcgtcc gtgaccaggt
     6661 ccagcacctg ctcgaagccg ttgcgtccac cgtagtaagg gtccggcacg gccgtctcgc
     6721 tgcgaccggc cgcatactcg agcaacaggc gcaactcggc gcgggcgtgg tccggcgccg
     6781 cacgccgcag gtccagcagg ttgtcctcgt ccatcgcgag gatcaggtcg aagagttcga
     6841 agtcctccac cgccacacgg cgcgcacgga tgccgtccag ctcgaagccc cgcgcggcgg
     6901 ccgccgcggc cgcgcgtgca tccggcgggt gcccctcgtg ataaccggcc gtgccggcgg
     6961 agtcgatgcg aatgcgctcg tgcaggccgg catcggcgac cagcttgcga aacacgcctt
     7021 cggccgtcgg cgacctgcag atattgccca ggcagacgaa cagtaccgac acctgttcgc
     7081 gcttctcttc cgttgccatt tgtttcttcc cggcgttgcc cggccccacc ggggccggaa
     7141 ccgtcattct acccgcagcg cgccgccgtt tcggccgcca gggcgcgcat gagccccaac
     7201 agcggctatc atccggacca tggacaacgg gatcaccctg gagcggctgc aatcgctggt
     7261 cggcacggag ctgcgctacc agggaatacg ctgcatgctg gtcgacgtgc tcgaagagcc
     7321 gcccgtgctg gtgctgcgcc cgctcggcgc cgagccggtg atccaggcgg acaatttcgg
     7381 caagccgatg cgccacgcgc cgcagctctt cgagctgccg gtgttcggcc cggacggggt
     7441 gagtctctcc gccgaactgc agctgatcag ggttcccggg ggcttgccga cagcaggcga
     7501 ttgacgcgcg ccaggtcggc cggcgtatcg atccccgccg gcggcgcctc cgtcgccacg
     7561 tccacctgga tgcgcatgcc gagccacagg gcgcgcagct gttccagccg ctcgagctcc
     7621 tccagggcac acggcggcgc ggccgccagc cgccgcaggg cgccgacgcg gtagctgtac
     7681 agtccgatgt gccgcaacgc gcccgcgaag cgctgctggg acgccaggcc ggcggcggcc
     7741 ccgtcacgat gccaggggat cggcgcgcgg ctgaagtaca gcgcggcgcc gtcctgccgc
     7801 gccgccagct tgaccacgtt ggtgtcgagg taatcgtcca gcgagcggat gggcgtaccc
     7861 agcgtgccga catcggcatc ggtgcgcagc aacagctcgg ccacctgccg aatgagcgcc
     7921 ggcggcaaca gcggctcgtc gccctgcacg ttgaccacca cctcggcgtc gtcccagccg
     7981 cgccgtgcgg cgacctccgc gatccggtcc gtgccggagg gatgggcggc atcggtcatg
     8041 cataccgcgg cgccgaaagc gcggcaggca tcggcaatgc ccgcatggtc ggtggcgacc
     8101 agcacctcca cagcacccgc ctccatggcg cgctgccaca cccactggat catcgctcgg
     8161 cccgcgatct gcagcagcgg cttgccgggc agccgcgtcg atgcgaagcg ggccggtatc
     8221 accacgcgaa agccgctcac cgccgctgct ccatggccag cagctccgcc tgcgatactt
     8281 cacgcgcctc ggccaccagc agcaccggca cgccgtcgcg aacgggccag gcgagccggt
     8341 cgccacgaca gaccagttca tgttcttcgc ggcggtgcac gagcgggccc ttgcaaatcg
     8401 gacaggcgag aatgtccagc aggcgatgat ccatgtttca agcttcccgc aagccgaggc
     8461 gctgcgccag tactgccgcc aggcgggctg catcggcgcc aaagtccggc gtcaccggca
     8521 cctcccagca gctggccgcc gccggccggc ccgaacagcg ggcggcgtcc ttcgaggtca
     8581 tcagcaccgg ccggccgtcc gccggcgcca ggagctcggc cggcacggac gcgtgatcgg
     8641 ccagcgggtg gcgcacgagc tgcagcccct gcccctccag catcgcgaaa aaccgctccg
     8701 gatcgccgat gccggccacc gcatgcacgg gccccggagc aaactccgcc aggggccggc
     8761 gcagcccgcc gtcgagccgg accgctacct cgccggccag gcgcatggtc agcgcgcccg
     8821 gcggacaggc gccgccgccg ttgcacacca ccaggtccac cgtcgccagc cgcgtcggcg
     8881 gttcgcgtag cggcccggcc ggcaggcagc ggccgttgcc gaagccgcgg cgcccgtcga
     8941 cgaccacgat ctcgacgt
//
LOCUS       DAOMPG010000087         8892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3369_length_8892_cov_4.579269, whole genome shotgun sequence.
ACCESSION   DAOMPG010000087 DAOMPG010000000
VERSION     DAOMPG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3369_length_8892_cov_4.579269"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLS34_06320"
     CDS             <1..1450
                     /locus_tag="PLS34_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP87117.1"
                     /translation="NRVDQIELSPLWQRAERLAAEFGLVATGYEPEHGALARTVQFAL
                     VHLFHPSTDVYSCPLAMTDGAARTLLDSGNQALVARAVSRLTSRDPARFWTSGQWMTE
                     STGGSDVGASETIARRQGDGWALWGRKWFTSAATSQVALTLARPEGNPPGGRGLALFF
                     VETRDDGGRLNRIEVLRLKDKLGTRKVPTAELMLDGTPAIPVAGTDNGIRQIVPMLTM
                     TRTWNSVCAASFMRRAVALARDYARRRQAFGRPLARQPLHVQTLAMLQAEYEAAFHLC
                     FELIHLLGRAEHGEADDDGRDLLRVMTALTKLTTGKQAVAVSTEAIECFGGAGYVEDT
                     GLPVIVRDSQVLPIWEGTTNVLSLELLKACGNGAPLAVLRRRIASCARACKDDRLLAA
                     VSLAGTAADRALAWHRSASASGRAALESHARGFALTLGRAVALALMCEHAQWSLEHEQ
                     DPRARAAALAFAASRISQLDGIEPDDAALLAN"
     gene            complement(1476..1829)
                     /locus_tag="PLS34_06325"
     CDS             complement(1476..1829)
                     /locus_tag="PLS34_06325"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRP87118.1"
                     /translation="MKEKQVCNLAAKTCVPCEGGVKPMSATEAREMLVQLHQDWSLNE
                     DATEIRRDFKFKGFYRTMGFVNAVAWLANNENHHPDMEVGWGHCLVRFQTHSINGLSE
                     NDFICAAKVDALLPQ"
     gene            complement(1826..2629)
                     /gene="phhA"
                     /locus_tag="PLS34_06330"
     CDS             complement(1826..2629)
                     /gene="phhA"
                     /locus_tag="PLS34_06330"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRP87119.1"
                     /translation="MGKGTKYVAKVPDENGFIHYTDDEHQIWHELITRQKSALPGRAC
                     AEYIDALDLLDLPEDRVPQCEEVSRVLRKHTGWEVAAVPALINFTDFFNLLANKRFPA
                     ASFIRTRQEMEYLQEPDIFHEIFGHTPLLTDPRFAAFTHAYGKAGAAARKEDRPMLAR
                     LYWFTVEFGLIHRPGEGLRTYGAGVVSSIGETVYALDSTEPQRKPFDPVDVLRTPYRI
                     DIFQPIYFVIESFDTLFEVAQMDLLGLIEQARKLGMHPPAFPPKEAATA"
     gene            complement(2756..3976)
                     /gene="ispG"
                     /locus_tag="PLS34_06335"
                     /gene_synonym="gcpE"
     CDS             complement(2756..3976)
                     /gene="ispG"
                     /locus_tag="PLS34_06335"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRP87120.1"
                     /translation="MNPVRRRPSLQVKVDHVRIGGDAPVAVQSMTNTDTADIEGTVRQ
                     VAELARAGSELVRVTVNTREAAAAVPVIRERLYAMGLNAPLVGDFHFNGHKLLAEVPE
                     CGVALSKYRINPGNVGRGSKRDPQFASMIEVACRNDRPVRIGVNWGSLDSDLLTRLMD
                     ENSKLPEPRDAHAVTQEAMIRSALDSAERAVELGLPRDRIILSCKVSGVQDLIAVYRD
                     IAARCDYPLHLGLTEAGMGSKGIVASTAGMAVLLQEGIGDTIRVSLTPEPGGDRSQEV
                     IVAQEMLQTMGLRSFTPMVIACPGCGRTTSTFFQELAQQIQGHIRHRMPEWKKRHEGV
                     EEMTVAVMGCVVNGPGESKHANIGISLPGTGERPVAPVYVDGEKTVTLKGDGIAAEFQ
                     ALVDDYVERRYARK"
     gene            complement(3981..5735)
                     /locus_tag="PLS34_06340"
     CDS             complement(3981..5735)
                     /locus_tag="PLS34_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRP87121.1"
                     /translation="MTLLRALTSTLLLCLPITAGAASADAELEAFLEAEWQRTLEANP
                     TWASSLGDRRWNDRWPDLSPEAIAASHEADLAALERLEEFDSDALSTARRLDKEMYRR
                     VLDESIQHYRYRRHLVPFNQRGGPQNAYETVERLRLVTVQDYEDWLARVRAFGDLGDQ
                     NLALLAAGVESGVVHPRIIAERIAAQLEAQAALDVEQSPWAKAFLELPADLPAAERER
                     LSREGLAAVRDVVLPAIRRLDDFFRTRYLPATRASVGVWDQPDGREYYAARARHFTTT
                     DLTPREIHDLGLSEVARIRSEMEAIREEVGFEGDLAAFFEHLRTDPSFYYDTPEALFE
                     AYLATSKRVDPELVKLFGRMPRMPYGVRPIPDAIAPDTTTAYYSRPAADGSRAGYYYV
                     NLYRPEVRPKYEIEALTIHEAVPGHHFQIALAMELEGLPAFRRFGGVTAYVEGWALYA
                     ESLGPELGMYQDPYSKFGQLTYEMWRAVRLVVDTGIHEFGWSRQQAIDYFRDNAAKTE
                     ADIVNEIDRYIAWPGQALAYKIGELKFKELRARAAAAQGEAFDIRAFHDLVLSNGAIP
                     LDSLERLVDEWLAEGQGS"
     regulatory      5837..5933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            6088..7179
                     /gene="gcvT"
                     /locus_tag="PLS34_06345"
     CDS             6088..7179
                     /gene="gcvT"
                     /locus_tag="PLS34_06345"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRP87122.1"
                     /translation="MGDRTPLHGEHERLGARLVDFGGWDMPLHYGSQVEEHHAVRSDA
                     GLFDVSHMTVVDLEGEGVRAWLRGLLANDVARLQTPGKALYSCMLNEDGGVIDDLIVY
                     FLAEGHFRMVVNAATRAKDLAWLERQAQGAAVTVRERPELAMIAVQGPDARHKAAQLL
                     PEQARGAALELGAFTAGQFGDLFVARTGYTGEDGWEIILPGAQAEDFWRRLVGAGLRP
                     CGLGARDTLRLEAGMNLYGQDMDESVSPLESGLGWTVAWEPAGRDFIGRTALEAQRSA
                     GPARKLVGLLLEDRGVLRAHQRVVTPAGDGEVTSGSFSPTLGRSIAFARVPKEAGERL
                     QVEVRGRLLDARVVRPPFVRNGKPRIQLD"
     gene            7216..7602
                     /gene="gcvH"
                     /locus_tag="PLS34_06350"
     CDS             7216..7602
                     /gene="gcvH"
                     /locus_tag="PLS34_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRP87123.1"
                     /translation="MSKIPAELRYTRTHEWLRQEDDGSVTVGISDHAQAALGDLVFVE
                     TPEAGRQVKSGEACAVVESVKAASDVYSPIGGEIIAGNEALATQPELLNTDPYGEGWI
                     MKIRPVGSAETSALLDANAYQATLED"
     gene            7610..>8892
                     /gene="gcvPA"
                     /locus_tag="PLS34_06355"
     CDS             7610..>8892
                     /gene="gcvPA"
                     /locus_tag="PLS34_06355"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRP87124.1"
                     /translation="MPFIPHTQDDIREMLDAIGVADIEQLFDEIPPGLKVEALAGVPP
                     ALGEMEIQRLMRERAALDAGHLNFIGAGAYEHHIPAAVWEITTRGEFYSAYTPYQAEA
                     SQGTLQLIYEYQSMLASLTGMDVSNASLYDGASAMAEAALMAVRANRSSKSARILVPA
                     TVNPAYRRVAASIAGGQGLQFETVPYCAETGATLPEALRRWDDQDVTALVIQQPNFFG
                     GYEDIAALTDWAHARGALVIAVVNPISLALLTPPGEWGEGGADIACGEGQPLGVPLSS
                     GGPYFGFLTCRNALVRQMPGRIVGRTVDLDGREGFALTLQAREQHIRRSKATSNICTN
                     QGLLVTAATIYMSLLGAEGLARVAAACVRNTAELVGQLESIEGVRRAFAGPFFHESVL
                     RLDQPVAPLLEALAERGILGGYDLSHDYPELGNALL"
BASE COUNT         1320 a         2994 c         3156 g         1422 t
ORIGIN      
        1 caaccgcgtc gaccagatcg agttgtcgcc gctgtggcag cgcgccgagc gcctggccgc
       61 ggaattcggc ctggtggcga ccggctatga gccggagcac ggcgcgcttg cgcgtaccgt
      121 gcagtttgcg ctggtgcacc tgttccaccc ttccaccgac gtctacagct gtccgctggc
      181 gatgaccgac ggtgcggctc gcaccctgct ggactccggc aaccaggcgc tggtggcccg
      241 ggccgtgtcg cgcctcacca gccgcgaccc cgcccgcttc tggaccagcg gccagtggat
      301 gaccgagtcc accggcggat cggacgtcgg cgcgtcggag acgatcgccc gccggcaggg
      361 cgacggctgg gccctctggg gccgcaagtg gttcacctcg gccgcgacct cgcaggtggc
      421 gctcaccctg gcgcggccgg aaggcaatcc gccgggcggg cgcggcctgg cgctgttctt
      481 cgtcgagacg cgcgacgacg gcggtcggct gaaccgcatc gaggtgctgc ggctgaagga
      541 caagctcggc acgcgcaagg tgccgaccgc cgagctgatg ctcgacggca cgccggcgat
      601 cccggtggcg ggcacggaca acggcatccg gcagatcgtg ccgatgctca ccatgacacg
      661 cacctggaac agcgtgtgtg cagcgtcctt catgcggcgc gccgtggcgc tggcgcggga
      721 ttacgcgcgc cgccgccagg ccttcggccg gccgctggcg cgccagcccc tgcacgtgca
      781 gacgctggcc atgctgcagg ccgaatatga ggcggcgttt catctctgct tcgagctgat
      841 ccacctgctt gggcgggccg agcatggcga ggccgacgac gacggccgcg acctgctgcg
      901 cgtcatgacg gcgctgacca agctcaccac gggcaagcag gcggtggccg tgtccacgga
      961 ggcgatcgag tgcttcggcg gcgcgggcta cgtcgaggac accgggctgc cggtgatcgt
     1021 gcgcgacagc caggtgctgc cgatctggga gggcaccacc aacgtgctgt cgctcgagct
     1081 gctgaaggcc tgtggcaacg gcgcgccgct ggccgtgctg cgccggcgca tcgcctcatg
     1141 cgcgcgcgcc tgcaaggatg acaggctgct cgcggccgtc agtctcgcgg gcaccgctgc
     1201 cgatcgtgca ctcgcctggc atcgatcggc gtccgcatcc gggcgcgccg cgctcgagtc
     1261 gcacgcgcgc ggctttgcgc tgacgctggg ccgggcggtc gcgcttgcgc tgatgtgcga
     1321 gcacgcgcaa tggtcgctgg aacacgagca ggatccgcgc gcgcgcgccg ccgcgctggc
     1381 gttcgcagcc tcgcgcatct cgcagctgga cgggatcgag ccggacgacg cggccctgct
     1441 ggcgaactag cggcgcgccg gcgtccggcc gggactcact gcggcaacag ggcgtcgacc
     1501 tttgcggcgc agatgaaatc gttttccgac aggccgttga tggagtgcgt ctggaagcgc
     1561 accaggcagt ggccccagcc cacttccatg tccgggtggt ggttctcgtt gttggccagc
     1621 caggccacgg cattgacgaa gcccatcgtc cggtagaagc ccttgaattt gaaatccctg
     1681 cgaatctcgg tggcgtcttc gttcaatgac cagtcctggt gcagttgcac cagcatctcg
     1741 cgagcctcgg tggcgctcat cggcttgacg ccgccctcgc agggcacgca ggtcttagcg
     1801 gcgagattgc acacttgttt ttctttcatg cggtggccgc ctccttgggc gggaatgctg
     1861 gcggatgcat gccgagcttg cgggcctgct cgatcagtcc gagcaggtcc atctgggcga
     1921 cctcgaacag cgtgtcgaag ctctcgatga cgaagtagat cggctggaag atgtcgatcc
     1981 ggtacggcgt gcgcagcacg tccaccgggt cgaagggctt gcgctgcggc tccgtgctgt
     2041 ccagtgcata gacagtctcg ccgatggacg agaccacacc cgcaccgtag gtgcgcaggc
     2101 cctccccggg ccgatggatg aggccgaact cgacggtgaa ccagtacagg cgcgccagca
     2161 tcggccggtc ttccttgcga gccgcggcgc cggccttgcc gtaggcgtgc gtgaaggcgg
     2221 cgaagcgcgg gtcggtgagc agcggcgtgt gaccgaagat ctcgtggaag atgtcgggct
     2281 cctgcaggta ctccatctct tgccgcgtgc ggatgaagct ggccgccgga aagcgcttgt
     2341 ttgccagcag gttgaagaag tcggtgaagt tgatcagtgc cggcacggcc gcgacttccc
     2401 agccggtgtg cttgcgcagg acccggctca cttcctcgca ctgtggtacg cggtcttccg
     2461 gcaggtcgag caggtcgagc gcgtcgatgt actccgcgca ggcgcgcccg ggaagcgcgc
     2521 ttttctggcg cgtgatgagt tcgtgccaga tctggtgctc gtcatccgtg taatggatga
     2581 agccgttttc gtccgggacc ttggccacgt atttcgtgcc cttgcccatg catcgctccg
     2641 ggtgattgtc taaccgcgca ttttaccggc gcggaggggg acgatgcatg acgcgccgcg
     2701 gcagtattgg ggacattccc ctaattgccc tgcaaaaaag gggaatgtcc ccaattcact
     2761 tgcgggcgta gcggcgctcg acgtagtcgt ccaccagcgc ctggaactcg gcggcgatgc
     2821 cgtcgccctt cagcgtcacg gtcttctcgc cgtcgacgta gaccggtgcg accggccgct
     2881 cgccggtgcc cggcaggctg atgccgatgt tggcgtgctt gctctcgccc ggcccgttca
     2941 cgacgcagcc catcaccgcc acggtcattt cctcgacgcc ctcgtgacgt tttttccact
     3001 ccggcatgcg gtgccgaatg tggccctgga tctgctgcgc gagttcctgg aagaaggtgc
     3061 tggtggtccg gccgcagcca gggcaggcga tcaccatggg cgtgaaggag cgcaggccca
     3121 tggtctgcag catttcctgg gcgacgatga cttcctggct gcggtcgccg ccgggctcgg
     3181 gcgtcagcga tacgcggatg gtgtcgccga tgccctcctg cagcagcacg gccatgccgg
     3241 cggtggaagc cacgatgccc ttgctgccca tgcccgcctc ggtgagcccg agatgcaggg
     3301 ggtagtcgca gcgcgccgca atgtcgcggt acacggcgat caggtcctgc acgccgctga
     3361 ccttgcagga caggatgatg cgatcgcgcg gcaggccgag ctcgaccgcc cgctccgcgc
     3421 tgtcgagcgc cgagcggatc attgcttcct gcgtcacggc atgtgcgtcg cgcggctcgg
     3481 gcagcttcga gttctcgtcc atcaggcggg tgagcaggtc cgagtccagg ctgccccagt
     3541 tgacgccgat gcgcaccggc cggtcgttac ggcaggcgac ctcgatcatg ctggcgaact
     3601 gcggatcgcg cttgctgccg cggccgacgt tgccggggtt gatgcggtac ttggacagcg
     3661 ccacgccgca ctcgggcacc tcggccagca gcttgtggcc gttgaagtgg aaatcgccga
     3721 ccagcggcgc gttcaggccc atggcgtaga gccgctcgcg gatgaccggc accgcggcag
     3781 ccgcctcgcg ggtgttgacc gtgacgcgca ccagttcgga accggcgcgc gcgagctcgg
     3841 cgacctggcg gacggtgccc tcgatgtcgg cggtgtcggt gttggtcatg gactgtacgg
     3901 cgaccggcgc gtcgccaccg atgcggacgt gatctacctt gacctggagc gaaggtcgcc
     3961 tgcgtaccgg gttcatccgg tcaactccct tgcccctcgg ccagccattc gtcgaccagc
     4021 cgctccaggc tgtcgagcgg aatggcgccg ttggacagca ccaggtcgtg gaaggcacgg
     4081 atgtcgaagg cctcgccctg cgccgccgca gcgcgtgccc gcagttcctt gaacttcagc
     4141 tcgccgatct tgtaggccag cgcctggccg ggccaggcga tgtagcggtc gatctcgttg
     4201 acgatgtcgg cctcggtctt ggcggcgttg tcgcggaaat agtcgatggc ctgctgccgg
     4261 ctccagccga actcgtggat cccggtgtcc actaccaggc ggacggcacg ccacatctcg
     4321 taagtcagct ggccgaactt cgaatagggg tcctggtaca tgcccagctc cgggccgagg
     4381 ctctccgcat acagcgccca gccctcgacg taggcggtga cgccgccaaa gcgccggaag
     4441 gcgggcagcc cctcgagctc catggcaagc gcgatctgga agtggtgccc ggggacggcc
     4501 tcgtgaatgg tgagcgcctc gatctcgtac ttggggcgta cttccgggcg gtacaggttg
     4561 acgtagtaat agccggcgcg gctgccgtcg gccgcgggcc gcgaatagta ggccgtggtc
     4621 gtgtccggcg cgatcgcgtc cgggatcggc cgcacgccgt aaggcatgcg cggcatgcgc
     4681 ccgaacagct tgaccagctc cgggtcgacc cgcttgctgg tcgccaggta ggcctcgaac
     4741 agcgcctcgg gcgtgtcgta gtagaagctc gggtcggtgc gcaggtgctc gaagaacgcc
     4801 gccaggtcgc cctcgaagcc gacctcttcg cgaatcgcct ccatctcgga ccggatgcgt
     4861 gccacttcgc tcagcccgag gtcgtgaatt tcgcgcggcg tcaggtcggt ggtggtgaaa
     4921 tggcgcgcgc gggccgcgta gtattcacgg ccgtccggct ggtcccagac gccgacgctg
     4981 gcgcgcgtcg caggcaggta gcgggtgcgg aaaaagtcgt ccagccgccg gatcgccggc
     5041 agcacgacat cgcgcaccgc cgccaggccc tcccgggaca gccgctcgcg ctcggcggcg
     5101 ggcaggtccg ccgggagctc aaggaaagcc ttggcccacg ggctctgttc gacgtcgagc
     5161 gcggcctggg cctcgagctg cgccgcgatg cgctcggcga tgatgcgtgg gtgcacgacg
     5221 ccggactcga cgccggcggc cagcagcgcc aggttctggt ccccgaggtc gccgaaggcc
     5281 cgcacgcgcg ccagccagtc ttcgtagtcc tgcacggtca cgaggcgcag ccgctcgacg
     5341 gtctcgtagg cgttctgcgg gccgccgcgc tggttgaacg gcaccaggtg gcggcggtag
     5401 cggtagtgct gaatactctc gtcgagcacc cggcggtaca tttccttgtc cagccggcgg
     5461 gcggtcgaca gcgcgtcgga gtcgaattct tcgaggcgct cgagtgcggc caggtcggcc
     5521 tcgtgcgacg ccgcaatggc ctccggcgaa agatccggcc agcggtcgtt ccagcggcgg
     5581 tcgccgaggg acgaggccca ggtcgggttg gcctccaggg tgcgctgcca ctcggcctcc
     5641 aggaaggcct ccagttcggc atcggcggac gccgcgccgg ccgtgatcgg caggcacagg
     5701 agcagggtgg aagtgagtgc gcgcagcagc gtcatcgtgg actccggcgg atcggtcggc
     5761 aggggggcaa tgatcaccgt cccgacccgg actcgcaagg gagcccggcc ctcgggtatc
     5821 atccgccctc caacgcatag tccccggcgg agagaccccg gccagccggc ccggggcgcc
     5881 gaaggcgcaa gtaccgcccg gaaacgctca ggcaaaagga cgccggggat gagttggctc
     5941 tggagagcgg ccgcacgcag ggcccaccga aggtgaccgg cttcgcaggc gcgcagcgca
     6001 gcggggctga atctctcagg ttcccggaca gagaggcggc aggcggcgcc tgccgttgtg
     6061 ctgtttgagc tttgggagac gcgcgttatg ggcgacagga cacctcttca cggcgagcat
     6121 gagcgcctcg gcgcgcggct ggtcgatttc ggcggctggg acatgccgct gcactacggc
     6181 tcgcaggtcg aggagcacca cgcggtgcgc agcgatgccg ggctgttcga cgtctcgcac
     6241 atgaccgtgg tggacctcga gggcgagggc gtgcgcgcct ggctgcgggg cctgctggcc
     6301 aacgacgtgg cgcggctgca gacgccgggc aaggcgctgt acagctgcat gctcaacgag
     6361 gacggcggcg tcatcgatga cctgatcgtc tatttcctcg ccgagggtca tttccggatg
     6421 gtggtcaacg cggcgacccg cgccaaggac ctggcctggc tggagcgcca ggcgcagggg
     6481 gccgccgtca ccgtccggga gcgccccgag ctggcgatga tcgccgtgca gggcccggac
     6541 gcacgccaca aggccgccca gctgctgccg gagcaggccc gtggcgccgc actcgagctc
     6601 ggggcgttca cagccgggca gttcggcgac ctgttcgtcg cgcgcaccgg atacaccggc
     6661 gaggacggct gggagatcat cctgcccggc gcgcaggccg aggacttctg gcgccgcctg
     6721 gtgggcgcgg ggctgcgccc ctgcggactc ggcgcgcgcg acacgctgcg gctcgaggcg
     6781 ggcatgaacc tgtacggcca ggacatggac gagtcggtca gcccgctcga atccggcctg
     6841 ggctggaccg tggcctggga gccggccggg cgcgatttca tcggccgcac ggccctggag
     6901 gcacagcgca gcgccggccc cgcacgcaag ctggtcggct tgttgctgga ggaccgcggc
     6961 gtgctgcgcg cgcaccagcg ggtagtcacc ccggccggcg acggcgaggt caccagcggc
     7021 agcttctcgc cgacgctcgg ccgctcgatc gcgttcgcgc gcgtgccgaa ggaggccggc
     7081 gagcgactcc aggtggaggt acgcggccgg ttgcttgacg cccgcgtagt caggccgccg
     7141 ttcgtgcgca acggcaagcc tcgcattcaa ctcgactgaa gcttttcacc cgacccggta
     7201 ttcgagaggc ccgcgatgag caagattcct gccgaactga ggtacacccg cactcacgag
     7261 tggctgcggc aagaggacga cggcagcgtg accgtcggca tttctgacca cgcccaggcg
     7321 gcgctgggcg acctggtgtt cgtcgagacg ccggaggccg ggcgccaggt gaagtccggc
     7381 gaggcctgcg cggtggtgga gtcggtcaag gccgcctcgg acgtctacag cccgatcggc
     7441 ggcgagatca ttgcgggcaa cgaggcgctt gccacgcagc ccgagcttct gaacaccgat
     7501 ccctacggtg agggctggat catgaagatc cggcctgtcg ggagcgccga aacctctgcg
     7561 ctgctggacg cgaatgcata ccaggcgact ctcgaggact gatcccgcga tgccattcat
     7621 accgcacacg caggacgata tccgcgaaat gctcgatgcc atcggcgtcg ccgacatcga
     7681 gcagctgttc gacgagattc cgccgggcct gaaggtcgag gcgctggccg gcgtgccgcc
     7741 tgcgcttggc gagatggaga tccagcggct gatgcgcgag cgagctgcgc tcgatgcggg
     7801 ccacctgaac ttcatcggcg ccggcgccta cgagcaccac attccggcgg ccgtgtggga
     7861 aatcactacc cggggagagt tctacagcgc ctacacgccg taccaggccg aggccagcca
     7921 gggcacgctg cagctcatct acgagtatca gtccatgctg gcgtcgctga ccggcatgga
     7981 cgtatcgaat gcctcgctgt acgacggcgc ctcggcgatg gccgaggctg cactgatggc
     8041 ggtgcgcgcc aatcgcagct cgaagtccgc gcgtattctc gtacccgcca cggtcaatcc
     8101 cgcctaccgc cgtgtcgccg ccagcatcgc gggcgggcag ggcctgcagt tcgaaaccgt
     8161 gccctactgc gcggaaaccg gcgccaccct gccggaggcc ctgcgccgct gggacgacca
     8221 ggacgtcacg gcactggtca tccagcagcc gaacttcttc ggcggctacg aggacatcgc
     8281 cgccctgacc gactgggcac acgccagggg tgcgctggtg attgccgtgg tcaacccgat
     8341 cagcctcgca ttgctgacgc cgcccggcga gtggggcgag ggcggcgccg acatcgcctg
     8401 cggcgagggt cagccgctgg gcgtgccgct cagctcgggc gggccctact tcggctttct
     8461 cacctgccgc aacgccctgg tgcgccagat gccggggcgc atcgtcggcc gcaccgtgga
     8521 cctcgacggc cgcgagggct tcgcgctcac cctgcaggcg cgcgagcagc acatccgccg
     8581 ttccaaggcg acctcgaaca tctgcaccaa ccagggcctc ctggtcaccg ccgcaaccat
     8641 ctacatgagc ctgctgggag ccgagggcct ggcgcgcgtc gccgccgcct gcgtgcgcaa
     8701 caccgctgag ctggtcggcc agctggaatc catcgagggg gtgcggcggg cattcgcggg
     8761 gccgttcttc cacgagtcgg tgctgcgcct cgaccagccc gtggcaccct tgctggaggc
     8821 gctcgcggag cgtggcatcc tcggcggtta cgacctgtcg cacgattacc cggagctcgg
     8881 caacgcgctg ct
//
LOCUS       DAOMPG010000088         8880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3374_length_8880_cov_4.903116, whole genome shotgun sequence.
ACCESSION   DAOMPG010000088 DAOMPG010000000
VERSION     DAOMPG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_8880_cov_4.903116"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..394
                     /locus_tag="PLS34_06360"
     CDS             44..394
                     /locus_tag="PLS34_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRP87125.1"
                     /translation="MASLPPDAEARVRRVVDAIPPGRVATYGEVAREAGLGRRARFVG
                     RVLARLPRGHRLPWHRVLCAGGRLAFEPGSAAFRRQRERLEQEGVRFKGNAVDLGRHG
                     WRASLDELLWAPHD"
     gene            460..2442
                     /locus_tag="PLS34_06365"
     CDS             460..2442
                     /locus_tag="PLS34_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87126.1"
                     /translation="MSYFVTGGSGFIGRRLIRKLLQRDGKVYVLMLERELDNMDKLRE
                     FWGEEAQRVVPIVGNLAEPKLGISAADRKKLKGQVGHFFHLAAIYDMNADLEVQLKVN
                     VEGTRQAVKFAEAIGAGCFHQVSSIASAGTYDGIFREDMFEQATGLEHPYFRTKHDSE
                     GIVRRECQIPWRIYRPGMVVGDSRTGEIEKIDGPYYFFKLIQKLRRALPGWMPIVGIE
                     GGRINLVPVDWLVAAMDHIAHAPGEDGKCFHLTDPNPMRIGEVLNLFARAAHAPEMSA
                     RLNARMFGIVPSYIWKTVASLPPVKRVVDSVLHDLGIPRSVMGYINYPTRFDSRETER
                     LLKGTDIKLPHLGDYAWRLWDYWERNLDPDLFVDRSLRGAVGGKTVLVTGASSGIGQA
                     TAFRMAEAGAKVIMVARGEEALRETQADIDKRGGEAHWYTCDLTSLEDIDRLVAQVLQ
                     DHGHVDVLVNNAGRSIRRSVALSFDRFHDFERTMQLNYFGSLRMTMGLLPVMMERRRG
                     HVINISSIGVLSNAPRFSAYVASKAALDAFTRCAASELSHKGVSFTTINMPLVRTPMI
                     APTKIYENVPTISPEEAAELVADAVIHKPKRIATRLGISAQILHLLFPKAMEISMNTA
                     FQMFPDSAAAKGAKEDQAAASADQIALAQLTKGIHW"
     gene            2447..2962
                     /locus_tag="PLS34_06370"
     CDS             2447..2962
                     /locus_tag="PLS34_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP87127.1"
                     /translation="MKAKAPFVCFSHGKESGPWGTKIAAMADLARDRGLAVESLDYRG
                     MDDPAERVARLLQYCAGIDRPLVLVGSSMGGHVAASASAQLADVRGLFLIAPAFYIPG
                     YEELTPEPAARRITIVHGWRDDVVPAANSIRFAAKHRAGLHLIDGDHRLSDHVFEINR
                     FFDWFLDDVTS"
     gene            complement(3010..3813)
                     /locus_tag="PLS34_06375"
     CDS             complement(3010..3813)
                     /locus_tag="PLS34_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014611437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87128.1"
                     /translation="MSEFSGNVVVVTGASEGIGRALCRALAPQGPKLVLAARNAERLE
                     SLAAECRAAGAEVLVVPGDLTSEAQCRALVDAAVERFGRIDTLVANAGRTMWARLDEI
                     RDPGVFREVMELNYFSLVWLTMAALPHLKAARGRIVPVASVAGLTGVPERSGYAASKH
                     AVVGFFDSLRIELAGSGVSVTTVCPDFVVTEIHRRALDGEGRPLVDSPMQEGKIMTAD
                     ECAALMVRAMAARRRLLITSTRGKLGRFLKIFAPGVIDRIAARAIAERR"
     gene            3918..4556
                     /locus_tag="PLS34_06380"
     CDS             3918..4556
                     /locus_tag="PLS34_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRP87129.1"
                     /translation="MDDEAQARAYAEADFEEPNARFAGMCAGFVAALGPGAAVLDLGC
                     GPGDITLRIAAANPAVEVHGLDGSPAMLAFGEAALAANPGLRGQVRFVEGLVPDAALP
                     RARYEAVVSNSLLHHLHDPAGLWRMIRARGMAGAPVLVMDLMRPASEAAAQALVDEYA
                     AGEPEVLRRDFYNSLLAAFEPAEVREQLAAAGLAQLVVEAVSDRHLVVRGRL"
     gene            complement(4567..>4745)
                     /locus_tag="PLS34_06385"
     CDS             complement(4567..>4745)
                     /locus_tag="PLS34_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRP87130.1"
                     /translation="ESALLVNPFDTIGVADRILTAAEMPLAERRERHAAMMQVLRDND
                     IHAWRKRFLAALDG"
     assembly_gap    4746..4755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4756..5937)
                     /locus_tag="PLS34_06390"
     CDS             complement(<4756..5937)
                     /locus_tag="PLS34_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRP87131.1"
                     /translation="MSRLIAISNRVAAPSGGKSAGGLAVGILAALRQSGGIWFGWGGK
                     TCQGEPSEASLERRGEITFATIDIDARDFDGYYNGYCNGSLWPLFHYMLGIFKYQRDH
                     YEAYQRVNQTFVRKLRPLLREDDRIWVHDYHLIPLAAELRRAGAHQPIGFFLHVPFPG
                     FDVLRTLPGHRQLLSALAQYDVVGFQTVLDCENFLGGLRAAGLGGERGPDGRIAVLDR
                     HIRVDAFPIGIDVEEAEQQAEASMGSAAVQRLAHDIGERELVIGVDRLDYSKGLPERF
                     RAFETLLERHPGSRRQLVFLQIASPTRTGVRAYQDIRHELERSAGHINGRFAEPDWTP
                     IRYINRGISRKMLMGYFRVAKIGLVTPVRDGMNLVAKEFVAAQDPADPGVLVLSRLAG
                     AA"
     gene            complement(5934..7736)
                     /locus_tag="PLS34_06395"
     CDS             complement(5934..7736)
                     /locus_tag="PLS34_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRP87132.1"
                     /translation="MPTPNDPSLELAVIGNCQVGALVDETGRMVWACMPRFDSNPVFC
                     ALLMGGQQQVERGVFEVVLEDFVRAEQRYVVNTPILETLLYDAHGGCIKIVDFAPRFE
                     QFERTFHPVMFIRQLVPVAGNPLVTVRLRPAGHYGAEDARQTFGSNHVRYVLPDMVLR
                     LTTDASLTYVRSERPFRLSRPVSFILGPDESLRESVAHTSRLYFSHTRSYWQDWVRGL
                     AIPFEWQDAVIRAAITLKLCSFDDTGAIIAAMTSSIPEAPGTARNWDYRYCWLRDSFF
                     VVHALNRLGATLTMEAYLGWILNVAASGDGNGLQPVYGIGGETELTESVVEHLPGYRG
                     MGPVRVGNQAFEQVQNDVYGAVVLSSAQAFFDHRLTTAAGREQLELLEQIGLRAFELF
                     DQPDAGIWEYRGRARVHTFSSIMCWAACDRLARICAHLGETGRAGTWRGRADHIHEVI
                     CARAWDEEQQCFTESFEGKSLDASMLLISDLGFLAADDPRFRSTVECIEKHLRRGKHM
                     FRYGEADDFGEPESAFNICTFWYINALAQIGRKDEARELFEEMLGCRTRLGLLSEDYD
                     VRSGQLWGNYPQTYSLVGIILGAIRLSKPWEDAF"
     gene            complement(7720..8481)
                     /gene="otsB"
                     /locus_tag="PLS34_06400"
     CDS             complement(7720..8481)
                     /gene="otsB"
                     /locus_tag="PLS34_06400"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRP87133.1"
                     /translation="MNEPSSLPAWDSCWALFLDVDGTLLEIMAHPDEVRAAPRLRRLL
                     GTAAAWLGGAVALVSGRSIASLDAVFHPLRLPAAGLHGLERRDAGGRVHYPTGYAERI
                     AAARGALLDFVQSAAGLLLEDKGAALALHYRGAPALEQECRRRIELAREAAGEDFHVQ
                     HGKMVYELKPSGRDKGTAVSDFMAEAPFRGRRPVFVGDDVTDEDGFRAVNALGGISIR
                     VGDSDRSAAQRAAADVAAILDWLNRCPEAGDADTE"
     gene            8578..>8880
                     /locus_tag="PLS34_06405"
     CDS             8578..>8880
                     /locus_tag="PLS34_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87134.1"
                     /translation="MYPWHALWRLALLLTRKLLFLWVRTRKLEAQLQRLKALADQPVC
                     FVLEHPAFADLAVLEQECIRNGLPRPSAGLSFDGTREWRAVAYLRRKPGRRGKAGRL"
BASE COUNT         1428 a         3075 c         3034 g         1333 t
ORIGIN      
        1 gccaggacct cgccgcccgg ctgaaggcct gagcggcggc gcgatggcca gcctgccgcc
       61 cgacgcggaa gccagggtac ggcgcgtggt cgacgcgatc ccgcccggcc gcgtggccac
      121 ctacggcgaa gtggcgcgcg aggcggggct gggccggcgc gcgcgcttcg tcgggcgcgt
      181 gctggcgcgc ctgccgcgcg gccaccggct gccctggcat cgggtgctgt gcgccggcgg
      241 gcggctggcc ttcgagcccg gcagcgcagc cttccgacgc cagcgcgagc ggctcgagca
      301 ggagggggta aggttcaagg gcaacgccgt ggacctgggg cgccacggct ggcgcgcgtc
      361 gctcgacgag ctgctgtggg cgccccacga ctagccccgt cgcctgctaa tatccgccct
      421 tcgcggtcaa caagaacgat cctccagggg agtcccgaca tgagctactt cgtcaccggc
      481 ggttccggat tcatcggccg gcgcctcatc aggaaattgc tgcagcgcga cggaaaggtc
      541 tacgtcctga tgctcgagcg tgagctcgac aacatggaca agctgcgcga gttctggggc
      601 gaggaagcgc agcgggtcgt ccccatcgtc ggcaacctcg ccgagccgaa gctggggatc
      661 tcggcggccg accgcaagaa gctcaaggga caggtcgggc atttcttcca cctggcggcg
      721 atctacgaca tgaacgccga cctcgaagtg cagctcaagg tcaacgtcga gggcacgcgc
      781 caggcggtca agtttgccga ggccatcggc gccggttgct tccaccaggt cagctccatc
      841 gcctcggccg gcacctacga cggcatcttc cgtgaggaca tgttcgagca ggccaccggg
      901 ctggagcatc cctacttccg caccaagcac gactccgagg gcatcgtgcg gcgcgagtgc
      961 cagattccgt ggcgcatcta ccgccccggc atggtggtcg gcgattcccg caccggcgag
     1021 atcgagaaga tcgacgggcc ttattatttc ttcaagctca tccagaagct gcgccgtgcg
     1081 ctgccgggct ggatgcccat cgtcggcatc gagggcggcc gcatcaacct ggtgccggtg
     1141 gactggctgg tggcggcgat ggaccacatc gcacatgcgc cgggcgagga cggcaagtgc
     1201 ttccacctga ccgaccccaa cccgatgcgt atcggcgagg tgctcaacct gttcgcgcgc
     1261 gccgcgcatg cccccgagat gagcgcgcgc ctcaacgccc gcatgttcgg catcgtgccc
     1321 agctacatct ggaagacggt cgccagcctg ccgccggtca agcgcgtggt cgacagcgtg
     1381 ctgcacgacc tcggcatccc gcgctcggtg atgggttaca tcaattaccc gacgcgcttc
     1441 gactcgcgcg agaccgagcg gctgctcaag ggcaccgaca tcaagctgcc gcacctgggc
     1501 gactacgcct ggcggctgtg ggactactgg gagcgcaatc tcgaccctga cctgttcgtg
     1561 gaccgcagcc tgcgcggcgc agtcggcggc aagaccgtgc tggtcaccgg cgcgtcgtcc
     1621 ggtatcggcc aggccaccgc cttccgcatg gcagaggctg gcgcgaaggt catcatggtg
     1681 gcgcgcggcg aggaagcgct gcgcgagacc caggccgaca tcgacaagcg cggcggcgag
     1741 gcgcactggt acacctgcga cctcacgagc ctggaggaca tcgaccggct ggtcgcgcag
     1801 gtgctccagg accacggcca cgtggacgtt ctggtcaaca acgcggggcg ctccatccgg
     1861 cgcagcgtgg cgctgtcctt cgaccgcttc cacgacttcg agcgcaccat gcagctcaac
     1921 tatttcggct cgctgcgcat gaccatgggg ctgctgccgg tgatgatgga gcggcgccgc
     1981 gggcacgtca tcaacatctc ttccatcggt gtgctgtcga acgcaccgcg cttctccgcc
     2041 tacgtggcgt ccaaggcggc gctcgatgcc ttcacgcgct gcgcggcctc ggaactgtcg
     2101 cacaagggcg tgagcttcac caccatcaac atgccgctgg tgcgcacgcc gatgatcgcg
     2161 ccgacgaaga tctacgagaa cgtgccgacc atcagcccgg aggaagcggc ggagctggtg
     2221 gccgacgcgg tgatccacaa gcccaagcgc atcgcgaccc ggctcggcat ctcggcgcag
     2281 attctccacc tgttgttccc gaaagcgatg gagatcagca tgaacacggc cttccagatg
     2341 ttcccggact cggccgcggc caagggcgca aaggaagacc aggctgcggc gtcggccgac
     2401 cagatcgcgc tggcgcagct caccaagggc atccactggt agcggcatga aggcgaaggc
     2461 gccgttcgtc tgcttttctc acggcaagga gagcgggccg tggggcacca agatcgcggc
     2521 catggcggac ctggcccgcg accgcggact ggccgtcgag agcctcgatt accgcggcat
     2581 ggacgatccc gccgagcggg tggccaggct cctgcagtac tgcgccggca tcgatcgccc
     2641 gctggtgctg gtgggctcga gcatgggcgg gcacgtcgcg gcgtcggcct cggcgcagct
     2701 ggcggacgtg cgcggcctgt tcctgattgc gcccgccttc tacatcccgg gctacgagga
     2761 actcaccccg gagccggcgg cgcggcgcat caccatcgtg cacggctggc gggacgacgt
     2821 cgtgccggcc gccaacagca tccgcttcgc cgcgaagcat cgcgccggac tgcacctgat
     2881 cgacggcgat catcgcctca gcgaccacgt cttcgagatc aaccgcttct tcgactggtt
     2941 cctcgacgac gtgacgagct gattggggac attccccaat tcgcgaaatt ggggaatgtc
     3001 cccaatcagc tagcggcgct cggcgatggc ccgggcggcg atccggtcga tcacgccggg
     3061 cgcaaagatc ttcaggaagc ggcccagctt gccgcgcgtg gaggtgatca gcagccgccg
     3121 ccgcgccgcc atggcacgca ccatcagcgc ggcgcattcg tcggccgtca tgatcttgcc
     3181 ctcctgcatg gggctgtcga ccagcggccg gccttccccg tccagtgcgc gccggtggat
     3241 ctcggtgacc acgaagtccg gacagaccgt ggtgacggaa acgccgctgc cggccagctc
     3301 gatgcgcagc gagtcgaaaa agccgaccac ggcgtgcttg ctggcggcgt agccgctgcg
     3361 ctcgggcacc ccggttaagc ccgccacgct ggctaccggc acgatgcggc cgcgcgcggc
     3421 cttcaggtgc ggcagcgcgg ccatggtcag ccacaccagc gagaaatagt tcagttccat
     3481 gacctcccgg aacacgccgg gatcgcggat ctcgtccagc cgggcccaca tggtgcggcc
     3541 cgcgttggcg accagggtgt cgatgcgccc gaaacgctcg accgcagcgt ccacgagcgc
     3601 gcggcactgc gcctcgctgg tcaggtcgcc cggcaccacc agcacctcgg caccggccgc
     3661 gcggcactcc gccgccagcg actcgagccg ctcggcgttg cgggccgcca gcaccagctt
     3721 cggtccctgc ggcgcgagcg cccggcagag cgcgcgcccg atgccctcgg aggcgccggt
     3781 gaccacgacg acgttcccgc tgaattcgct catgagcctc tccgctttca tggccaacgc
     3841 aatacgctac cattcgccgg tattgcgaga cagtcgcggg gagcgcgaac gtgcggagaa
     3901 tcccggaacc ggaactgatg gacgacgagg cgcaggcgcg cgcctacgcc gaggccgact
     3961 tcgaggaacc caacgcgcgc ttcgcgggca tgtgcgccgg cttcgtggca gcgctcgggc
     4021 cgggcgccgc cgtgctcgac ctgggttgcg ggccgggcga tatcaccctg cgcatcgcgg
     4081 cagccaatcc ggccgtggag gtgcacggcc tggacggctc gccggcgatg ctggccttcg
     4141 gcgaggcggc gctggcggcc aacccggggc tgcgcggcca ggtgcgcttc gtcgaggggc
     4201 tggtacccga cgccgcgttg ccccgcgccc gctacgaggc ggtggtgtcg aacagcctgc
     4261 tgcatcacct gcacgacccc gccgggctgt ggcggatgat ccgtgcccgc ggcatggccg
     4321 gtgcgcccgt gctggtgatg gacttgatgc gaccggccag cgaggcggcc gcgcaggcgc
     4381 tggtcgacga atacgccgcc ggcgagcccg aagtgctgcg cagggatttc tacaactcgc
     4441 tgctggccgc gttcgagccg gccgaggtcc gcgaacagct ggcggccgcc ggtctcgccc
     4501 agctggtcgt cgaggcggtg agcgaccgcc acctggtcgt gcgcggccgc ctctgaaatc
     4561 cgctgctcaa ccgtccagcg cagccaggaa gcgcttgcgc caggcgtgga tgtcgttgtc
     4621 gcgcagcact tgcatcatgg ccgcatggcg ctcgcggcgc tccgccagcg gcatctcggc
     4681 ggccgtcagg atcctgtcgg cgacgccgat ggtgtcgaag gggttcacca gcagggcgct
     4741 ctcgcnnnnn nnnnnggcgg cgccggcgag ccgtgacaac acgagcaccc cggggtcagc
     4801 agggtcctgg gccgctacga attccttcgc gaccaggttc atgccgtccc ggacgggcgt
     4861 caccagcccg atcttcgcga cgcgaaaata gcccatcagc attttccgcg agatgccgcg
     4921 gttgatgtag cggatgggtg tccagtccgg ctcggcgaag cgcccgttga tgtggccggc
     4981 cgatctctcc agctcgtggc ggatgtcctg gtaggcccgc accccggtgc gcgtcgggga
     5041 ggcgatctgc aggaacacca gttggcgacg gctgcctgga tggcgctcca gcaacgtctc
     5101 gaaggcgcgg aagcgctcgg gcagtccctt gctgtagtcc agccggtcca cgccgatgac
     5161 cagttcgcgc tcgccgatgt catgcgccag ccgctgcacg gcggccgagc ccatcgacgc
     5221 ctcggcctgt tgttccgcct cctcgacgtc gatgccgatg ggaaaggcgt cgacccggat
     5281 gtgccggtcg agcacggcga tgcgcccgtc gggacctcgc tcgccgccca ggccggcagc
     5341 gcgaaggccg cccaggaagt tctcgcaatc gagcactgtc tggaagccga ccacgtcgta
     5401 ctgcgccagc gcgctcagca gctggcggtg gcccggcagc gtgcgcaaca cgtcgaagcc
     5461 cgggaacggc acgtgcagga agaagccgat cggctggtgc gcgcccgccc ggcgcagctc
     5521 tgccgccagc gggatgagat ggtagtcgtg cacccagatc cggtcgtctt cgcgcagcag
     5581 cggccgcagc ttgcgcacga atgtctggtt gacccgctgg taggcctcgt aatgatcgcg
     5641 ctggtacttg aagatgccga gcatgtagtg aaacaacggc cacagggaac cgttgcagta
     5701 accgttgtaa tagccgtcga aatccctcgc gtcgatgtcg atggtggcga aggtgatctc
     5761 tccgcgacgc tccaggctgg cctcggaggg ctcgccctgg caggtcttgc cgccccagcc
     5821 gaaccagatt ccgccggact ggcgcagcgc ggcgaggatg ccgacggcga ggccgccggc
     5881 agacttgccg cccgacgggg cggcgacgcg gttggaaatt gcgatgagcc tgctcagaac
     5941 gcgtcctccc acggcttgct gaggcggatt gcaccgagaa tgatgccgac caggctgtag
     6001 gtctggggat agttccccca cagctggcca ctgcgcacgt cgtaatcctc ggacagcagg
     6061 ccgagccgcg tgcggcaccc cagcatttcc tcgaacagct ctcgcgcctc gtccttgcgg
     6121 ccgatctggg cgagcgcgtt gatgtaccag aacgtgcaga tgttgaaggc gctctccggc
     6181 tcgccgaagt cgtccgcctc gccgtagcgg aacatgtgct tgccgcgccg cagatgcttt
     6241 tcgatgcact cgacggtgct gcgaaagcgc ggatcgtccg ccgcgagaaa gcccaggtcg
     6301 ctgatcagca gcatactggc gtcgaggctc ttgccttcga agctctccgt gaaacactgc
     6361 tgttcctcat cccaggcacg cgcgcagatc acttcgtgaa tgtggtccgc ccggccacgc
     6421 caggtcccgg cgcggccagt ctcgcccagg tgcgcgcaga tccgcgccag gcgatcgcag
     6481 gccgcccagc acatgatgct ggaaaaggtg tgcacgcgtg cgcggccgcg gtactcccag
     6541 atgcctgcat ccggctggtc gaacaactcg aaggcacgca gcccgatctg ctcgagcagc
     6601 tccagttgct cgcggccggc ggccgtggtc agccggtgat cgaaaaaggc ctgggccgac
     6661 gacagcacga cggcgccgta gacgtcgttc tgcacctgct cgaaggcctg gttgccgacg
     6721 cgcaccggcc ccatgccgcg atagccgggc aggtgctcga cgacggactc ggtcagctcc
     6781 gtctcgccgc cgatcccgta cacgggctgc agcccgttgc cgtcgccgga ggccgccacg
     6841 ttgaggatcc agccgaggta ggcctccatg gtgagcgtcg cgccgagacg gttcagcgcg
     6901 tgcaccacga agaagctgtc gcgcagccag cagtagcggt agtcccagtt gcgggcggtt
     6961 cccggcgcct ccggaatcga gctcgtcatg gcggcgatga tcgcgccggt atcgtcgaag
     7021 gaacacagct tcagggtgat ggccgcccgg atcactgcat cctgccactc gaagggaatc
     7081 gcgaggccgc gcacccagtc ctgccagtag ctgcgggtat gggagaagta aagccggctg
     7141 gtgtgcgcca ccgattcccg caggctctcg tcgggcccga ggatgaagct gacgggccgc
     7201 gacaggcgaa acgggcgctc gctgcgcacg taggtcagcg aggcgtcggt agtcaggcgc
     7261 agcaccatgt ccggcagcac gtagcgcacg tggttgctgc cgaaggtctg ccgggcgtcc
     7321 tccgcgccgt agtggccggc ggggcgcaac cgcaccgtga ccagtggatt gccggcaacc
     7381 ggcacgagct ggcggatgaa catcaccggg tggaaagtac gttcgaattg ttcaaagcgc
     7441 ggcgcgaagt cgacgatctt gatgcagccg ccgtgagcgt cgtacaggag cgtctccagg
     7501 atgggcgtgt tgaccacgta gcgctgctcc gcgcgcacga agtcctcgag caccacctcg
     7561 aacacgccgc gctcgacctg ctgctgcccg cccatcagca gcgcacagaa caccgggttg
     7621 ctgtcgaagc gcggcatgca ggcccacacc atgcggccgg tctcgtcgac cagcgcgccg
     7681 acctggcagt tgccgatcac ggccagttcc agggaggggt cattcggtgt cggcatcgcc
     7741 agcctccgga catcgattca gccagtcgag tatcgcagcc acgtcggcgg ctgcccgttg
     7801 cgcggcggag cggtccgagt cgccgacgcg gatcgaaatg ccgccgagtg cgttgaccgc
     7861 acgaaagccg tcctcgtcgg tgacatcgtc cccgacgaat acgggacggc ggccccggaa
     7921 gggcgcctcg gccatgaagt cgctcaccgc ggtgcccttg tcccggccgg agggcttgag
     7981 ctcataaacc atcttgccgt gctggacatg aaagtcctcg ccggccgcct cccgggccag
     8041 ctcgatgcgc cgccggcact cctgctcgag cgcgggggcg ccccggtaat gcagtgcgag
     8101 cgcggcgccc ttgtcctcca gcagcaggcc ggccgcggac tgcacgaaat ccagcagtgc
     8161 gccgcgggca gcggcgatgc gctccgcata cccggtgggg taatgcaccc ggccgcccgc
     8221 atcccggcgc tccagcccat gcaggccggc tgccggcagc cgcagcggat ggaacaccgc
     8281 gtccaggctg gctatggaac gtccgctgac gagcgcgacg gcgccgccaa gccaggcggc
     8341 ggcagtgccc aggagccgcc tcagccgcgg cgcggcgcgc acttcgtccg ggtgcgccat
     8401 gatctccagc agggtcccgt ccacgtccag aaacagtgcc cagcaggaat cccacgccgg
     8461 cagcgaggac ggctcgttca tgccgcaagg gtaatgtgca cgacggtgga actccaactg
     8521 cgggccggaa acccgcactg acgcgcaacc cgcccgacag gtaaactgcg gggccttatg
     8581 tacccgtggc atgcactctg gcggctggcg ttactgctga cgaggaagct gctgttcctc
     8641 tgggtgcgta cccgcaagct cgaggcccag ctgcagcgac tcaaggcgct cgcggaccag
     8701 cccgtctgct tcgtgctgga gcacccggcc ttcgccgatc tcgccgtgct cgagcaggaa
     8761 tgcattcgca acggcctgcc gcggccgtcc gccgggctga gcttcgacgg gaccagggaa
     8821 tggcgcgccg tcgcctacct gcgacgcaag cccggccgca ggggcaaggc cgggcggctc
//
LOCUS       DAOMPG010000089         8875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3379_length_8875_cov_4.363265, whole genome shotgun sequence.
ACCESSION   DAOMPG010000089 DAOMPG010000000
VERSION     DAOMPG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_8875_cov_4.363265"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLS34_06410"
     CDS             complement(<1..789)
                     /locus_tag="PLS34_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87135.1"
                     /translation="MNLTRPALGNPVAVIVAVLLVLLFGIISLLRMPVQMIPNIERPL
                     IEISTTWRAAAPEEVEAEIVEPQEDALRGLPGLELMESSASRGRGSISLTFDVNSSLE
                     RSLIEVMNRLNRVPSYPVDVDQPVVYAGRGQFGSAIAWFAIRPLPGNDRDITSYQDFV
                     EEVVQSRIERVAGVANSDIYGGRNREVRITFDPYRAAALGIDVPTLARMTGGNDDVSG
                     GFKDVGRRQYTIRFSGKYDLEQLRDMVLTWRDGQPVHLYDVADVA"
     gene            906..1574
                     /locus_tag="PLS34_06415"
     CDS             906..1574
                     /locus_tag="PLS34_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HRP87136.1"
                     /translation="MTRRIILVCLLALAASAADAQTRYVSDRLEITLRTGTSTQHSII
                     RMLPSGARLEALQTDAASGYTRVRTADGTEGWVLSRYLMDRPAAREQLESATGRVDTL
                     NARVAELTSQVEILRGERNALDAERSGLGTEAEELRAELERIKRVSASAMELDKANRE
                     LRTRLAAAEQAGDDLRMEVAELKRSTQRDWFMAGAGVLALGLVLGLVIPRLRLGRRSR
                     WGDL"
     gene            1580..1948
                     /locus_tag="PLS34_06420"
     CDS             1580..1948
                     /locus_tag="PLS34_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRP87137.1"
                     /translation="MKTFVILGALLGGLGVAAGAFGAHALRAQLEPRMLEVFETAVRY
                     QMIHALALLAAAWLVQQTEATAAHVAGWAFVAGVLLFSGSLYALVFTGLRVLGAVTPV
                     GGVAFLVGWAALAVAALRLR"
     gene            complement(1986..4343)
                     /gene="ppsA"
                     /locus_tag="PLS34_06425"
     CDS             complement(1986..4343)
                     /gene="ppsA"
                     /locus_tag="PLS34_06425"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRP87138.1"
                     /translation="MEQYILKLDELGMGDVERVGGKNASLGEMLANLEGLGVTVPSGF
                     ATTAAAYRDFMKQDGLADRIRDVLADLDVDDVEKLVETGAAIRGWIKDTPLPPKLHEE
                     IRRAWDEMAAGRDIAVAVRSSATAEDLPEASFAGQQETYLNVRGIDQVIERIHDVFAS
                     LFNDRAIAYRVHHQFDHNQVALSAGIQHMVRSDTGASGVMFTLDTESGFRDVVFITAS
                     WGLGEMVVQGAVNPDEFYVYKPALAAGKRALLRKTLGSKAIRMVYGEDGHGTRTQDVP
                     AADRRRFCLDADDIETLARHAVAIEKHYGCPMDIEWGKDGGDGRLYILQARPETVQSR
                     QGRSILRFRLKDRSTVIATGRSIGQRIGAGTARIIKDVKEMNRVQPGDVLVADMTDPD
                     WEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATSKVPDGKPVTVSCAEGD
                     TGFIFEGKLDYEEQTIELDRMPEIPVKIMMNVGNPDRAFDFAGIPHSGVGLARLEFII
                     NRMIGVHPRALLEFDALSEDLQHTIRQQMAGYDDPVEFFVEKLAEGIGTIGAAFAPHP
                     VIVRLSDFKSNEYANLIGGREYEPHEENPMLGFRGASRYVAESFRPCFELECRALKRV
                     REEMGLENVQVMVPFVRTVAEARRVVELLADNGLQRGEHGLKLIMMCEIPSNALLAEQ
                     FLEYFDGMSIGSNDMTQLTLGLDRDSSIIAELFDERDAAVKALLHMAISACTKQGKYI
                     GICGQGPSDHADFARWLLDEGIESISLNPDTVVETWMFLAGKSVS"
     gene            4413..5252
                     /locus_tag="PLS34_06430"
     CDS             4413..5252
                     /locus_tag="PLS34_06430"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRP87139.1"
                     /translation="MNHRTVFFVSDQTGVTAETLGHSLLTQFDGLKFRGVTVPFINTP
                     DKAKECVRRIKLTAEVEGSRPIVFATMVQDDLRDIIAESQGLVLDFFGAFIGPMEREL
                     GVKSSHATGKAHGVADQRSYTRRIDAMNYALEHDDGSTTRDYSKADLILVGVSRSGKT
                     PTCLYMALRYGVYAANYPLTDEEFERGKLPAVLERHRDRLFGLTIAPRRLQQIRNERR
                     PESNYASARQVSFELRSAEALFNRYGINHIDTTDSSVEEIASTVLDRTGLLNKLQDRQ
                     GPV"
     gene            complement(5305..6939)
                     /locus_tag="PLS34_06435"
     CDS             complement(5305..6939)
                     /locus_tag="PLS34_06435"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HRP87140.1"
                     /translation="MAARIVPTRPFPDQRPGTAGLRKKVRVFRQPHYLENFLQAVFDS
                     AGDFRRATLVAGGDGRYWNREALQVLFRVAAGNGVERIIVGRQGLLSTPAASHLIRLH
                     GAAGGFLLTASHNLGGPDGDFGVKFDTANGGQAPESLTEAAWRASLALREYRVVDIPA
                     PDLETLGSRQAGPLQIEVVDPVADYAALMESLFDFDRIRALLTGGRFRMRFDALNAVT
                     GPYAVEILERRLGAPGGSVVNAVPLPDFGGLHPDPNPVDAAHLVEWMSGPDGGDLAAA
                     SDGDGDRNMIVGPGMVVTPGDSLAVLAEHAREVPGYRQGLAGVARSMPTSRAVDRVAE
                     SLGIPCYETPTGWRYFCNLLDAGRVDLCGEESFGTSSSHAREKDGLWAVLFWLNIVAT
                     SGRSVTELVADHWRRFGRHAFARHDWFIADGQRAAEVMAGLGQRLDGLAGIKLADTTV
                     ERADNFTYRDPVDESVSANQGIRIFLADGSRLVYRLSGTGTEGATLRIYLERYLPPGA
                     SHRGTGPELTAALGRAAAELARIRETTGVEAPAGII"
     gene            7015..7455
                     /locus_tag="PLS34_06440"
     CDS             7015..7455
                     /locus_tag="PLS34_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HRP87141.1"
                     /translation="MLPRVTLPGSFTALMHLYESNYLRLHWLLEDVAALPDEQCSRVT
                     ADLPLHLSVIDRSRYTTTLRLTYFFEDDRDCVADPDLTVRVYHDAGLAEAMACRSRHL
                     HHALRRFDTSAGDELGRRWARNSMLNKWLEYCHDHGHRFHGAGA"
     gene            7452..8495
                     /locus_tag="PLS34_06445"
     CDS             7452..8495
                     /locus_tag="PLS34_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87142.1"
                     /translation="MSERADELPAGREESEEERLLNLFRNRAELKKAFSDLQKALRLA
                     EERLASQEAATRRAEERFHAIEQLLAQPGTGYTALVYFQLRALWRACHERLQIISDEL
                     RGRHEERQRREELMRFNQEKQRQLAALDQQLTQAKDEVEERLAGRNAARAELERAQGL
                     FAYFRRRSLSERLQQRQSELEASRLRLAELQDRRAAVSAEPWPEFSGLDNGTKREINV
                     FVIAAAQELYLLFATDDLARKARDANVNPIEEMRYGTEEECKALIGKIRKAVAVLGPS
                     RPSLADIEARAQSITKQAQFRSARETVPMAASVSRIDLPVRSSEGSSVRRIPLEVNVL
                     ADEYWDIYEVFIP"
     gene            complement(8485..>8875)
                     /locus_tag="PLS34_06450"
     CDS             complement(8485..>8875)
                     /locus_tag="PLS34_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87143.1"
                     /translation="VNNGIVLIDHVNNLRRAGMERDAALLQAGRDRLRPILMTVATTI
                     LGLLPLAVSATKVGGDEIGAPAYFPMARAIIGGLGFSTVTSLLLVPWTYALLDDVGRW
                     GRRVKAVARRSTGGGAAATARANPVRE"
BASE COUNT         1442 a         2931 c         3044 g         1458 t
ORIGIN      
        1 ggcgacgtcg gcgacgtcgt acaggtggac cggctggccg tcgcgccagg tcagcaccat
       61 gtcgcgcaac tgctcgaggt cgtacttgcc ggagaagcgg atggtgtact ggcggcggcc
      121 cacgtccttg aagccgcccg agacgtcgtc gttgccgccg gtcatgcggg cgagcgtcgg
      181 cacgtcgatc cccagcgcgg ctgcgcggta cgggtcgaag gtgatgcgca cttcgcgatt
      241 gcggccgccg tagatgtcgg aattcgccac gccggcgacg cgctcgatgc gcgactgcac
      301 cacctcttca acgaagtcct ggtagctggt gatgtcccgg tcgttgccgg gcagcggccg
      361 gatggcgaac caggcaatgg cgctgccgaa ctggccgcgt cccgcgtaga ccaccggctg
      421 gtcgacgtcg accgggtagc tgggcacgcg gttgagccgg ttcatgacct cgatcagcga
      481 gcgctcgagt gaggagttga cgtcgaaggt gagcgagatc gagccgcggc cgcggctggc
      541 gctggactcc atcaactcca ggcccggcag gccgcgcagc gcgtcctcct ggggctcgac
      601 gatctccgcc tcgacttcct cgggcgcggc ggcgcgccag gtcgtggaga tctcgatgag
      661 cggccgctcg atgttaggga tcatctgcac gggcatgcgc agcagggaaa tgatgccgaa
      721 cagcagcacc agcaacacgg ccacgatgac tgccacggga ttgcctagcg cgggtcgggt
      781 gaggttcact tcgcgggccg caggtgggta gggccgcgta ttgtaactcg ataattacgg
      841 tgcgctgccc ggggcgagtt ccggtatcat gcactccccc cacccaagga cctcgcggac
      901 ccgccatgac ccgccgaatc atcctcgttt gcctgttggc gctggccgcc tcggctgccg
      961 atgcccagac acgctatgta tctgaccggc ttgagataac gctgcgcacc ggtacctcga
     1021 cgcagcattc gatcattcgc atgctgccga gcggcgcgcg gctcgaggcg ctacagaccg
     1081 acgcggcgtc cggctatacg cgcgtgcgca ccgccgacgg caccgagggc tgggtgctga
     1141 gccgctacct gatggaccgg cctgcggcca gggaacagct ggagagtgcg accggacgcg
     1201 tcgatacact caatgcaagg gtggcggagc tcacctccca ggtggaaatt ctgcggggtg
     1261 agcgcaacgc gctggacgcg gagcgcagcg ggctgggcac ggaggccgag gaactccgcg
     1321 ccgagctcga gcggatcaag cgggtctctg cgtcggcgat ggagctcgac aaggccaacc
     1381 gcgaactgcg cacccgcctg gccgcagccg agcaggccgg cgatgacctg cgcatggagg
     1441 tcgccgagct caagcgcagc acgcagcgcg actggttcat ggcaggcgcc ggggtgctgg
     1501 cgctcggcct tgtattgggg ctcgtcattc cgcgactccg cctgggtcgg cggtcgcgct
     1561 ggggcgacct ctaggccccg tgaagacctt cgtcatcctc ggcgccctgc tgggcgggct
     1621 cggcgtcgcc gccggggcct tcggtgcgca tgcgctgcgc gcccagctgg agccacgcat
     1681 gctggaggtc ttcgagaccg cggttcgcta ccagatgatc catgcgctcg cgttgctggc
     1741 tgccgcatgg ctggtgcagc agaccgaagc gaccgcagca catgttgccg gctgggcgtt
     1801 cgtcgccggc gtgttgctgt tctcgggctc gctgtatgcg ctggtcttca ccgggctccg
     1861 cgtgctcggt gccgtcacgc cggtcggcgg cgtggccttc ctggtcggct gggccgcgct
     1921 ggccgtggcc gcgctgcggc tccgctgagg tcgccggcgc cggccggccg caaccgtccc
     1981 ggcgcctacg acaccgattt tccggccagg aacatccagg tctcgaccac ggtgtcggga
     2041 ttgagcgata tgctctcgat gccctcgtcg agcagccagc gcgcgaagtc cgcgtggtcg
     2101 gacgggccct ggccgcaaat gccgatgtac ttgccctgct tcgtgcaggc cgagatggcc
     2161 atgtgcagca gggccttgac ggccgcgtcc cgctcgtcga acagctccgc gatgatgctt
     2221 gaatcacgat cgaggccgag cgtcagctgg gtcatgtcgt tggagccgat ggacatgccg
     2281 tcgaaatact cgaggaactg ctccgcgagc agggcattgg acgggatctc gcacatcatg
     2341 atcagcttca agccgtgctc cccgcgctgc aggccgttgt cggccagcag ctcgacgacg
     2401 cggcgcgcct ccgccacggt gcgcacgaag ggcaccatga cctgtacgtt ttccagcccc
     2461 atctcctcgc gcacccgttt cagcgcccgg cactcgagct caaagcacgg ccggaagctc
     2521 tcagccacgt agcgcgaggc gccgcggaag ccaagcatgg ggttttcctc gtgcggctcg
     2581 tactcgcgcc cgccgatcag gttggcgtac tcgttcgact tgaagtccga gagccgcacg
     2641 atgacgggat gcggcgcgaa cgcggcgccg atggtgccga tgccctcggc cagcttctcg
     2701 acgaaaaact ccacggggtc gtcgtagccg gccatctgtt gccggatggt gtgctgcagg
     2761 tcttcggaca gcgcgtcgaa ttcgagcagg gcgcgcgggt ggacgccgat catgcggttg
     2821 atgatgaact ccagccgggc caggccgacg ccggaatgcg ggatgccggc gaagtcgaag
     2881 gcccggtccg ggttgccgac gttcatcatg atcttgaccg ggatttcggg catgcggtcg
     2941 agctcgatgg tctgctcctc gtaatcgagc ttgccctcga agatgaagcc ggtgtcgccc
     3001 tcggcgcagg acactgtgac cggcttgccg tccggcacct tgctggtggc gtcgccgcag
     3061 ccgacgacgg cgggaatgcc aagttcacga gcgatgatcg ctgcgtgaca ggtgcgtccg
     3121 ccgcggttgg tgacgatggc cgcggcacgt ttcatgaccg gctcccagtc cgggtcggtc
     3181 atgtcggcga ccagcacgtc gccgggctgc acgcggttca tttccttgac gtccttgatg
     3241 atccgcgcgg tgcccgcgcc gatgcgctgg ccgatgctgc gaccggtggc aatgacggtc
     3301 gagcggtcct tcagcctgaa gcgcaggatc gagcggccct ggcggctctg caccgtctcc
     3361 gggcgcgcct gcaggatgta cagtcggcca tcgccgccgt ccttgcccca ctcgatgtcc
     3421 atcgggcagc cgtaatgctt ctcgatcgcc acggcgtggc gcgccagcgt ctcgatgtcg
     3481 tcggcgtcga gacaaaagcg ccggcggtcc gcggcgggaa cgtcctgggt gcgggtgcca
     3541 tggccgtcct cgccgtacac catcctgatg gccttgctgc cgagcgtctt gcgcagcagc
     3601 gcgcgcttgc cggctgccag cgccggcttg tagacgtaga actcgtcggg gttcacggcg
     3661 ccctgcacga ccatttcacc gaggccccag gaagcggtga tgaacaccac gtcacggaag
     3721 ccggattcgg tgtccagcgt gaacatgacg ccgctggcgc cggtgtcgct gcgcaccatg
     3781 tgctggatgc cggccgacag ggcgacctgg ttgtggtcga actggtggtg cacccggtag
     3841 gcgatggcgc gatcgttgaa cagcgaggcg aacacgtcgt ggatgcgctc gatcacctgg
     3901 tcgataccgc ggacgttgag gtaggtctcc tgctggccgg cgaagctcgc ctccggcagg
     3961 tcctcggcgg tcgccgacga gcgtaccgcg acggcgatgt cgcggcccgc ggccatctcg
     4021 tcccaggcac gccggatctc ttcgtgcagc ttcggcggca gcggggtatc cttgatccag
     4081 ccgcggatgg ccgcgccggt ctcgaccagt ttctcgacgt catccacgtc gagatcggcg
     4141 agcacgtcgc ggatgcggtc ggccaggccg tcctgcttca tgaaatcccg gtaggcagcc
     4201 gcggtggtgg cgaaaccgct cggcacggtg accccgagtc cttcgaggtt ggcgagcatt
     4261 tcgccgagcg acgcgttctt gccgccgaca cgctcaacgt cgcccatgcc gagttcatcg
     4321 agcttcagaa tgtactgttc caccttggcc cccttgagtt tcccttctca gggaacggac
     4381 aatgcgcgct gccgcgacac acgggtaagg gaatgaatca tcgcaccgtt ttcttcgtgt
     4441 ccgaccagac aggcgtcacc gccgagacac ttgggcacag cctgttgacg cagttcgacg
     4501 ggctgaaatt tcgcggcgtg actgtgccat ttataaacac ccctgacaag gcgaaagagt
     4561 gcgtacgtcg cattaaactg accgcagaag tcgagggctc gcggccgatc gtgttcgcca
     4621 caatggtcca ggatgacttg cgagatatta tcgcggaaag tcaaggactt gtgctggatt
     4681 tctttggtgc tttcataggt ccgatggagc gcgagctggg ggtcaagtcg agccacgcga
     4741 ccggcaaggc gcacggcgtg gccgaccagc gctcctacac ccgccgcatt gacgcgatga
     4801 attacgcgct cgagcacgac gacggctcga ccacgcggga ctacagcaag gcggacctta
     4861 tcctggtcgg agtgtcgcgc tcgggcaaga cgcccacctg cctgtacatg gcgctgcgct
     4921 acggggttta cgcggcgaat tatccgctca ccgacgagga attcgagcgc ggcaagctgc
     4981 cggcagtgct ggaacgccat cgggaccgac tgttcgggtt gaccatcgcg ccgcgtcgcc
     5041 tgcagcagat tcgcaacgag cgccgtccgg agagcaacta cgcttcggcg cgccaggtga
     5101 gcttcgaact gcgctcggcc gaggcgctgt tcaaccgcta cggcatcaat cacatcgaca
     5161 ccacggacag ctcagtcgag gaaatcgcca gcaccgtgct ggatcgtacc gggctgctga
     5221 acaagcttca ggaccggcaa gggccagtat gaattgggga cattccccat ttccggcgcg
     5281 tcggaaatgg ggaatgtccc caattcagat gatgccggcc ggcgcctcga ctccggtcgt
     5341 ctcgcggatg cgagccagct cggcggctgc ccggcccagc gccgcggtca gttcggggcc
     5401 cgtgccccgg tgcgacgcgc cggggggcag ataccgctcc aggtagatgc gcagggtggc
     5461 gccttcggtc ccggtgcccg agaggcgata gaccaggcgc gagccgtccg caaggaatat
     5521 gcggatgccc tggttggcgg aaacactttc gtccaccggg tcccggtaag tgaagttgtc
     5581 cgcgcgctcg acggtggtgt cagcgagctt gatgccggcc aggccgtcga gccgctggcc
     5641 gagtcccgcc atcacttcgg ccgcgcgctg tccgtccgcg atgaaccagt cgtggcgtgc
     5701 aaaggcgtgg cgcccgaacc tgcgccagtg gtccgccacg agctccgtga ccgagcggcc
     5761 gctggtcgcc acgatattca accagaagag caccgcccag aggccgtcct tctcgcgcgc
     5821 atggctggag ctggtgccga agctctcctc gccgcacagg tccacccggc ctgcatccag
     5881 caggttgcag aagtagcgcc agccggtcgg cgtctcgtag cagggaatcc ccagcgactc
     5941 ggcgacgcgg tcgaccgccc ggctggtcgg catggagcgc gcgacgccgg ccagtccctg
     6001 ccgatagccc ggtacctcgc gggcgtgctc ggcgagtact gcgaggctgt cgcccggcgt
     6061 caccaccatg ccgggcccga caatcatgtt gcgatcgccg tcgccgtccg aggccgccgc
     6121 caggtccccg ccgtcgggac cgctcatcca ctcgaccagg tgagcggcgt ccaccgggtt
     6181 ggggtcggga tgcagaccgc cgaagtcggg caagggcacg gcattgacga cgctgccgcc
     6241 gggtgcgccg aggcgccgtt cgaggatctc cacggcgtag gggccggtga ctgcattgag
     6301 ggcgtcgaag cgcatgcgaa accgcccgcc ggtcaacagc gcgcgaatgc ggtcaaagtc
     6361 gaacagggac tccatcagcg ccgcgtaatc ggccaccggg tcaaccactt cgatctgcag
     6421 cggcccggcc tgccggctgc ccagtgtttc caggtccggc gcgggtatgt ccactacacg
     6481 gtattcgcgc aacgccagcg aagcgcgcca ggcggcctcg gtgagcgatt cgggcgcctg
     6541 gccgccgttg gcggtgtcga acttgacgcc aaaatcgcca tcgggaccgc ccaggttgtg
     6601 gctggccgtg agcaggaaac cgcccgcggc gccgtgcagg cggatgaggt gcgaggctgc
     6661 gggggtcgac agcaatccct gccggcccac gatgatgcgt tccacgccgt tccccgccgc
     6721 aacccggaac aggacctgca gggcctcgcg attccagtaa cgcccgtcgc cgcccgccac
     6781 cagcgtggcg cggcgaaaat ctccggccga gtcgaacacc gcctgcagga aattctcgag
     6841 gtagtggggc tggcggaata cccggacttt cttgcgcagt ccggccgtgc cgggtcgctg
     6901 gtccgggaaa ggcctggtgg ggacgatgcg cgcggccatg tgaggctctc cgttgcgtcg
     6961 gtgccacgaa atccctgtga tatattgccg cgcatgatag cagacggttc aatcgtgctg
     7021 ccgcgtgtaa cgctgccggg cagcttcact gcgctcatgc acttgtacga gagcaactat
     7081 ctgcgcctgc actggctgct ggaggacgtg gccgcgctgc cggacgagca gtgctcgcgc
     7141 gtcacggcgg acctgccgtt gcacctgtcg gtaatcgatc gcagccgtta caccaccacg
     7201 ctgcgcctga cttatttctt cgaggacgat cgcgactgcg tggcggatcc ggacctcacg
     7261 gtgcgcgtgt accacgacgc aggcctggcg gaggcgatgg cctgccggag ccgtcacctg
     7321 catcatgccc tgcggcgctt cgacacttcg gcgggcgatg agctcggccg gcgctgggcg
     7381 cgcaacagca tgctcaacaa gtggcttgag tactgccacg accacggcca ccggtttcac
     7441 ggggccggcg cgtgagcgaa cgcgcggatg agcttccggc agggcgggag gaatccgagg
     7501 aggagcgcct gctcaacctg ttccgcaacc gtgcggagct gaagaaagcc ttttccgacc
     7561 tgcagaaggc attgcgcctg gccgaggagc gactggccag ccaggaggcg gcgacacgcc
     7621 gcgcggagga acgcttccac gccatcgagc agctgctggc gcagcccggg accggttata
     7681 cggccctggt ctacttccag ctccgggcgt tgtggcgcgc ctgccacgag cgcctgcaga
     7741 tcatctccga cgagctgcgc gggcgccacg aagagcggca gcggcgtgag gaactgatgc
     7801 gcttcaacca ggaaaaacag cggcagctcg cggcgctcga ccagcagctc acgcaggcga
     7861 aggatgaagt cgaggaacgg ctggccgggc gcaatgctgc gcgtgccgag ctcgagcgcg
     7921 cgcaaggcct gttcgcctac ttcaggcgac ggtcgctgag cgagcgcttg cagcagcgac
     7981 agagcgagct cgaggcctcg cgcctgcggc tcgccgagct ccaggaccgg cgggcagcgg
     8041 tcagtgccga gccctggccg gaattttccg ggctggacaa cggcaccaag cgcgagatca
     8101 acgtgttcgt tatcgccgcg gcccaggaac tgtatctcct gtttgcgacg gacgacctgg
     8161 cccgcaaggc gcgcgacgcc aacgtcaatc cgatcgagga aatgcgctac ggcacggagg
     8221 aagagtgcaa ggcactgatc ggcaagatcc gcaaggcagt ggccgtgctc gggccgagcc
     8281 ggccgagcct cgctgacatt gaagcccgcg cccagtcgat caccaagcag gcccagttcc
     8341 gcagcgcgcg cgagaccgtg ccgatggcgg cctcagtgtc acgcatcgac ttgccggtgc
     8401 gctccagcga gggttcgtcg gtgcgccgca tccctctcga ggtcaacgtg ctggcggacg
     8461 agtactggga tatctacgag gttttcattc cctgaccgga ttcgcgcggg cggtcgccgc
     8521 cgcaccgccg ccggtggaac gtcgggcgac ggccttcacg cggcggcccc accgaccgac
     8581 gtcgtcgagc aacgcgtacg tccagggcac cagcagcaac gaggtgacgg tggaaaaccc
     8641 cagcccgccg atgatggcgc gcgccatcgg gaaataggcc ggcgcaccaa tctcgtcgcc
     8701 gcccaccttg gtggcgctga ccgccagcgg cagcaggccg agaatggtgg ttgcgaccgt
     8761 catgagaatc gggcgcagcc ggtcgcggcc ggcttgcagc agcgccgcat cacgttccat
     8821 tccggcgcgc cgcaggttgt tgacgtggtc gatcagcacg atgccgttat tcacg
//
LOCUS       DAOMPG010000090         8830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3403_length_8830_cov_3.526040, whole genome shotgun sequence.
ACCESSION   DAOMPG010000090 DAOMPG010000000
VERSION     DAOMPG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_8830_cov_3.526040"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLS34_06455"
     CDS             complement(<1..196)
                     /locus_tag="PLS34_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87144.1"
                     /translation="MNAACAVPGATRWLAALALVALVAGCGRAPEEQETRHRFTAMGT
                     LVDVTIWDAEPLAAEAAAREV"
     gene            complement(193..1158)
                     /gene="gshB"
                     /locus_tag="PLS34_06460"
     CDS             complement(193..1158)
                     /gene="gshB"
                     /locus_tag="PLS34_06460"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRP87145.1"
                     /translation="MNESRIRLGVVMDPIDRIKPAKDTTLAMLLEAQRRGYALEYLTQ
                     ADLWVLEGRLGMRLRAVTVRDDPEHWFELGPQREAHADELDVILMRKDPPFDMEYIYT
                     TFLLERAEEAGVLVVNKPAALRDVSEKAYTMWFPEFTPETMVTRSMADMRRFVARFGA
                     AVVKPLDGMGGRSIFVLRQGDPNTGVILETLTDHDRRYAMIQRYVPEIVAGGDRRILL
                     VDGEPYPHVLARIPAEGENRGNLAAGARGEGRDLTPREREICAAVGPVLRDKGILFAG
                     LDVIGDKLTEINVTSPTGVRELDRQYGDNICGRLFDAIERRLEAR"
     gene            1307..1705
                     /gene="pilG"
                     /locus_tag="PLS34_06465"
     CDS             1307..1705
                     /gene="pilG"
                     /locus_tag="PLS34_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HRP87146.1"
                     /translation="MVDADGSRSMNGLKVLVVDDSKTIRRTAETLLSKEGCEVYTAVD
                     GFDALSKVADHHPDIIFVDIMMPRLDGYQTCALIKHNKVFKSTPVIMLSSKDGLFDRA
                     RGRIVGSEQYLTKPFTRDELLSAIETHVKA"
     gene            1736..2107
                     /locus_tag="PLS34_06470"
     CDS             1736..2107
                     /locus_tag="PLS34_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP87147.1"
                     /translation="MATVLIVDDSPTEVHVLSGYLKKHGFETETAADGAEGIAKARAL
                     KPDLILMDVVMPGMNGFQATRELARAPDTAGIPIIMVTTKGLETDKIWGMRQGARDYL
                     VKPVTEAQLVEKVRTALGGHD"
     gene            2100..2666
                     /locus_tag="PLS34_06475"
     CDS             2100..2666
                     /locus_tag="PLS34_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP87148.1"
                     /translation="MTEAAPLSQLRESPFALLREMERRAWSTAGERGTAPDAPEWVGI
                     GFRLGDERFLAPRDEVREITACPTALARVPGAKEWVAGLTSLRGQLLTVIDLKAFLGG
                     GATRLGRDSRILVINHRELGAGLLVDEILGFRRYPETARAAAPAERTLRCSRYLAGAF
                     GSADELWPVFNLAALTESPSFLRAANDG"
     gene            2941..3954
                     /locus_tag="PLS34_06480"
                     /pseudo
     CDS             2941..3954
                     /locus_tag="PLS34_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            3951..>8830
                     /locus_tag="PLS34_06485"
     CDS             3951..>8830
                     /locus_tag="PLS34_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRP87149.1"
                     /translation="MISQPSPQLLWVTGELAGTLRDARIALEEFAERPEQAESIKRCA
                     EMLHQAHGALRIAEVYGASLLADEMEQLALHLHRNAAAGHSMEEGLDALSRAIVQLPP
                     YLDRVVTGGRDIPLVLLPLLNDLRAARGSPLLSENTLLLLNLPSDRQLLARGARPQPS
                     GEDIVVLAKKLRPRYQAALLNWIRGERPDSSLAVIADVSATFERAAAETSVYQLWWVL
                     GGVVEALRQGGLTASAAIKRLLGQVDRQIRRLVDEGEAGLAAAPPVELLNSLLFYVAR
                     SSTRGTRVNAIRSTFSLGDLLPDQEDLAEARDSLGGPSVQLMRTVAGAIREDLGKVKD
                     AFDLFARTGKVPAEGLEAQLEMLAKIGDTLGVLGLGDVRDEVQTEANRLHGLLERDRE
                     EAEEELLSVAAALLRVEDRLDERLMRLVLEEPAREPGDEAQEAADDELQNVAEALLRE
                     CLFNMARVRDAISERLAGPIDPQLADAVPGLLKAVTAALLILEHPRAVTVVERITSYV
                     RRMLEPGAEDMRREALDRLADAIVSLEYFMETIRAGRKDPVFMLENAEACLDALPFLE
                     DGAIPEYQEVASPPGDTVTLVEKQKTGSDTSFAKPGSDTGFAAQPRPETPAPEGAPTP
                     EQAPPTPVAAPAAPAPPAKPEVPVYEGEEHPDPELLELFIEEARELDESISMYFPRWV
                     ADGDAGELGTVRRNFHTLKGSGRMVGARLIGEYAWSIERLMNAMLERRVERTEPLVQF
                     LEHAVTFVPMLIEQLETGRPPAVDVQPWILQANAFADNRADAAETFQALLAAPAAPPP
                     VEAEAPAEAAAAPAEEAPPTEAAPGEAPAPVEETPAPEEAPVPEETPAPEEAPPAAEA
                     PAPEAPAVPEMDPVLRDIFTREASGHLEVLREYIAGCALRTAPYAVTEAAHRASHTLA
                     GSANMADVAPAVAVARPLNEHLRRLYDDHVGLSEAGLELVRRAVDTIAGVVEALTEGR
                     APEAADYDLAAEIRALHEDYQLRAEAGATVTLPEYRPPEEFDPEILALFCEEATEILE
                     EAEGAMASWRARPSSEEPVRALQRYLHTIKGSARLAGVRTIGDFSHDLENLFEALADG
                     SRVSEPALVEIAQQSLDAMHGMRDVAAAGHMPEIPAGLRERIAAALKGEEITPYVPPE
                     EEVAPVEEEAPAEAAPPAEAPAAPVEEEVPAAPAEEPTAPVEQEAPAPVYVPPAEYPP
                     PLITFPPERAPEPEPTPEPTPEPTPEPTPEAEPAVTPEPTPEPMPEPKPEPKPEPKPE
                     PLPPLAPEVPALRAGRAARQEVARVDAELLENLLNSAGEVGIYRSRLEEQLGSVQFNL
                     EELGATVVRLRDQLRKMEIETETQIIHRHQGDMDARGDFDPLELDRYSMIQQLSRALA
                     ETASDVGSIQQLIGERMREAETIIVQQSRAVSELQDGLMRTRLVPFNRHAQRLSRLVR
                     QVASEYGRRAELQVVGGTAELDRQIMDRMLGPMEHLLRNAVIHGIEPPEARLAAGKSD
                     TGRITVGVRREGAEVVVEVGDDGRGLDVPAIRQKALKLALIKRDDPFGEAEAAEVILR
                     PGFSTATELTQGAGRGVGTDVVASEVRQLGGSLEVRNRAGQGVTFEIRLPFTRAITQA
                     LVLRAGEEWYALPLPAV"
BASE COUNT         1380 a         3221 c         3108 g         1121 t
ORIGIN      
        1 cgacctcgcg cgccgccgcc tcggccgcga gcggctcggc gtcccagatg gtcacgtcga
       61 ccagcgtgcc catggcggtg aagcggtggc gggtttcctg ctcctcgggc gcgcgcccgc
      121 agcccgccac cagcgccacc agggcgagcg ccgccagcca gcgcgttgcg cccggcaccg
      181 cgcaggccgc gttcaacgcg cctccaggcg gcgctcgatg gcgtcgaaca ggcgcccgca
      241 gatgttgtcg ccgtactggc ggtccagctc gcgcacgccg gtcgggctgg tgacgttgat
      301 ctcggtgagc ttgtcgccga tgacatcgag gccggcaaac aatatgccct tgtcgcgcag
      361 caccggcccg accgcggcgc agatctcgcg ctcgcgcggc gtcaggtcgc ggccctcgcc
      421 gcgcgcgccg gccgccaggt tgccgcggtt ctcgccctcc gccggaatgc gcgccagcac
      481 gtgcgggtag ggctcgccgt ccaccagcag gatgcggcgg tcgccgccgg cgacgatctc
      541 cggcacgtag cgctggatca tggcgtagcg ccggtcgtgg tcggtgagcg tctcgaggat
      601 cacgccggtg ttcggatcac cctggcggag cacgaaaatg gagcgcccgc ccatgccgtc
      661 gagcggcttg accaccgccg cgccgaagcg cgccacgaag cggcgcatgt ccgccatgga
      721 gcgggtcacc atggtctcgg gcgtgaactc ggggaaccac atggtgtagg ccttctcgct
      781 gacatcgcgc agcgccgccg gcttgttgac caccagcacg ccggcctcct cggcgcgctc
      841 cagcaggaag gtcgtgtaga tgtactccat gtcgaagggc gggtccttgc gcatcaggat
      901 cacgtccagc tcgtcggcat gggcctcccg ctgggggccg agttcgaacc agtgctcggg
      961 gtcgtcgcgg acggtgaccg cgcgcagccg catgccgagg cgcccctcca gcacccacag
     1021 gtcggcctgg gtcaggtatt cgagcgcgta accccgccgc tgggcctcca gcagcatcgc
     1081 cagggtggtg tccttggcgg gcttgatgcg gtcgatcggg tccatcacga ccccgaggcg
     1141 gatgcggctc tcgttcaagg cctggctccc tggtttggca agcgtcaact atatcaagcg
     1201 tttggtggca cagcgcatgg cgggtgccgg ccggatatgt taaaaggccg ggcagtgttc
     1261 ggagtcaaaa caagcccgtg cgaggcggac ttcaagtgga gaacgaatgg tggacgcaga
     1321 cgggtcacga agcatgaacg ggctcaaggt gctcgtcgtg gacgacagca aaaccatccg
     1381 gcgcacggcc gagacgctgc tcagcaagga gggctgtgaa gtctacaccg cggtggacgg
     1441 cttcgacgcc ctgtcgaagg tcgccgacca ccacccggac atcattttcg tggacatcat
     1501 gatgccgcgg ctcgacggct accagacctg tgcgctgatc aagcacaaca aggtgttcaa
     1561 gtcgacgccg gtcatcatgt tgtccagcaa ggacggcctg ttcgaccggg cccgcggcag
     1621 gatcgtcggc tccgagcagt acctgacgaa gccgttcacg cgggacgagc tgctgtccgc
     1681 catcgagacg cacgtcaagg cctgactgcc ggcagcacag gcctgaggag gatgcatggc
     1741 cacggtactg atcgtcgacg actcccccac cgaggtgcac gttctcagcg gctatctcaa
     1801 gaagcacggc ttcgagaccg agacggccgc agacggcgcc gagggcatcg ccaaggcccg
     1861 cgcgctcaag ccggacctga tcctcatgga cgtggtgatg ccgggcatga acggcttcca
     1921 ggcgacccgc gagctggcgc gcgcgcccga caccgccggc atcccgatca tcatggtgac
     1981 caccaagggg ctcgagaccg acaagatctg gggcatgcgg cagggcgcca gggactacct
     2041 ggtcaagccg gtcaccgagg cccagctggt cgagaaagtg cgcaccgcgc tgggcggcca
     2101 tgactgaggc cgcgcccctc agccagctgc gtgagagtcc gttcgcgctg ctgcgcgaga
     2161 tggagcggcg cgcctggtcc acggccggcg agcggggcac ggcgcccgat gcaccggagt
     2221 gggtcggcat cggcttccgg ctcggcgacg agcgcttcct cgcgccgcgc gacgaagtgc
     2281 gcgagatcac cgcctgcccg accgcgctgg cgcgcgtgcc cggcgccaag gagtgggtcg
     2341 ccgggctgac cagcctgcgc ggccagctgc tcacggtcat cgacctgaag gccttcctcg
     2401 gcggtggcgc gacccggctc gggcgcgaca gccgcatcct ggtcatcaac caccgcgagc
     2461 tcggcgccgg cctgctggtg gatgaaatcc tcggcttccg gcgctacccg gaaacggcac
     2521 gcgccgcggc cccggcggag cgcacgctgc gctgcagtcg ctatctcgcc ggcgctttcg
     2581 gatcggccga cgagctctgg ccggtcttca acctcgcggc gctcaccgag agtccgtcgt
     2641 tcctgcgggc cgccaacgat ggctgagacg cgtccgcaca aggcttccag catggaatcg
     2701 catatgagac agaacgcgaa gggcgtcgcc ctgctgccgg tactgggggt catcctcgtg
     2761 ctggtcatcc tcatcgccgc ggggctgttc tggcgagcgc tgccggacac cgcctcgctg
     2821 gcctccgccg acggcctgcc ggtgtggctg gcgctggtgg cgctgctgct ggccatcgtg
     2881 ctggtggtgt cgctttggcg cggcgaggaa gcgcgccgcc gctcctatga gcacgcccgc
     2941 cagaacgagc gcaaccagga agccatcctg cggctgctgg acgagctcgg cagcctcgcc
     3001 gacggcgacc tgacggtgca ggcgaccgtc accgaggaca tcaccggcgc gatcgccgat
     3061 tccatcaact acgccatcga ggcgttgcgc ggcctggtgg ccaccatcaa ccagtcggcc
     3121 atcaagctgg acgccgccgc caagcagacc caggccaccg ccacccacat ggccgacgcc
     3181 agcgacaccc agctgcgcca ggccggcgac gccaccgagt ccaccgcgcg catggccgcc
     3241 tccattgagg cggtgtcggg cgacgccgag cgcgccgccg acgtggcgcg gcattccgtg
     3301 gacgtggcgc acaagggcgg cgccgccgtg cgccgcacca tcgaaggcat gaacgccatc
     3361 cgcgagacca tccaggacac ctccaagcgc atcaagcggc tcggcgagag ctcgcaggag
     3421 atcggcaaca tcgtcgagct gatcaacgac atcgccgagc agaccaacat cctggcgctg
     3481 aacgcctcca tccaggcctc catggccggc gaggcggggc gcggcttcgc cgtggtggcc
     3541 gacgaggtgc agcggctgtc cgagcgcgcc gccaacgcca cgcgccagat cgaggtgctg
     3601 gtgaagacca tccagtccga caccaacgag gccgtggtgt cgatggagcg cagcaccacc
     3661 gacgtggtgg gcggcgcgct gctggccgag aacgccggcg ccgcgctcga cgagatcgag
     3721 agcgtctcca accagatcgc caacctggtg cagaacatct cgcgctcggc gcgcgaccag
     3781 gccaccgccg ccggcgacat cacgcgcaac atgaacgtgc tgcgcgaagt cagcgagcag
     3841 accgcggacg ccaccggcgc cacctcccag tcgatcggca agctcgccga tctcgctgcg
     3901 cagctgcgca actcggtcgc cgggttcagg ctgccggagc cgccgcgcaa gtgatttcgc
     3961 agccgtcgcc gcaactgctc tgggtgaccg gcgaactcgc cggcacgctg cgcgatgcac
     4021 gcatcgcgct ggaggagttt gccgaacggc cggaacaggc ggagtccatc aagcgctgcg
     4081 ccgagatgct gcaccaggcg cacggcgcgc tgcgcatcgc cgaggtctac ggcgcctcgc
     4141 tgctggccga cgagatggag cagctggcgc tgcacctgca tcgcaacgcc gctgccggcc
     4201 actccatgga agagggcctc gacgcgctgt cgcgcgccat cgtgcagctg ccgccctacc
     4261 tcgaccgggt ggtgacgggc gggcgcgaca tccccctggt gctgctgccg ctgttgaacg
     4321 acctgcgcgc ggcgcgcggc tcgccgctgc tgtccgagaa cacgctgctg ctgctgaacc
     4381 tgccctccga ccgccagctg ctggcgcgcg gcgcgcggcc gcagccctcc ggcgaggaca
     4441 tcgtcgtgct ggcgaagaag ctgcggccgc gctaccaggc cgcgctgctc aactggatcc
     4501 gcggcgagcg ccccgacagc agcctcgcgg tcatcgccga cgtgtccgcc accttcgagc
     4561 gcgccgccgc cgagacctcg gtctaccagc tgtggtgggt gctcggcggc gtcgtcgagg
     4621 cgctgcgcca gggcgggctg accgccagcg ccgccatcaa gcggctgctc ggccaggtgg
     4681 accggcagat ccgccggctg gtcgacgagg gcgaggccgg cctggccgcc gcgccgccgg
     4741 tcgagctgct caacagcctg ctgttctacg tggcgcgctc ctcgacccgc ggcacccgcg
     4801 tcaacgccat ccgcagcacc ttcagcctcg gcgacctgct gcccgaccag gaggacctgg
     4861 cggaggcgcg cgacagtctc ggcggcccga gcgtgcagct gatgcgcacc gtggccggtg
     4921 cgatccgcga ggacctcggc aaggtcaagg acgccttcga cctgttcgcg cgcaccggca
     4981 aggtgcccgc cgagggcctc gaggcgcagc tggagatgct ggccaagatc ggcgacacgc
     5041 tcggcgtgct cggcctcggc gacgtgcgcg acgaggtgca gaccgaggcc aaccgcctgc
     5101 acggcctgct ggagcgcgac cgcgaggagg ccgaggaaga gctgctgtcg gtcgcggccg
     5161 cgctgctgcg cgtcgaggac cggctcgacg agcggctcat gcggctggtg ctggaggagc
     5221 cggcgcgcga gcccggcgac gaggcccagg aggccgccga cgacgagctg cagaacgtcg
     5281 ccgaggcgct gctgcgcgaa tgcctgttca acatggcgcg ggtgcgcgat gccatcagcg
     5341 agcggctcgc cgggccgatc gatccgcagc tggccgacgc cgtgccgggg ctgctcaagg
     5401 cggtcaccgc cgcgctgctg atcctcgagc acccgcgcgc cgtcaccgtg gtggagcgca
     5461 tcaccagcta cgtgcgccgc atgctcgagc ccggcgccga ggacatgcgc cgcgaggcgc
     5521 tcgaccggct ggccgacgcg atcgtcagcc tcgagtactt catggagacc atccgcgccg
     5581 gccgcaagga cccggtcttc atgctggaga acgccgaagc ctgcctcgac gcactgccct
     5641 tcctcgagga cggcgccatc cccgagtacc aggaggtcgc cagcccgccc ggcgacaccg
     5701 tcaccctggt cgaaaagcaa aaaacggggt cagacaccag tttcgcgaaa ccggggtcag
     5761 acaccggttt tgccgcgcag ccgaggcccg agacacctgc tccggagggc gccccgacac
     5821 cggaacaggc gcctccgacc ccggttgctg cgccggccgc gcccgcgccg cccgcgaagc
     5881 ccgaggtgcc ggtctacgag ggcgaggagc atcccgaccc ggagctgctc gagctgttca
     5941 tcgaggaagc gcgcgagctc gacgaatcga tctccatgta cttcccgcgc tgggtggccg
     6001 acggcgacgc cggcgaactc ggcaccgtgc ggcgcaactt ccacaccctc aagggcagcg
     6061 gccgcatggt cggcgccagg ctgatcggcg agtacgcctg gtccatcgag cggctgatga
     6121 acgccatgct cgagcggcgc gtcgagcgca ccgagccgct ggtgcagttc ctcgagcacg
     6181 ccgtcacctt cgtgccgatg ctcatcgagc agctcgagac cggccggccg cccgcggtcg
     6241 acgtgcagcc gtggatcctg caggccaacg ctttcgccga caaccgcgcg gacgccgccg
     6301 agaccttcca ggcgctcttg gccgccccgg ccgcgccgcc gcccgtggag gcagaagctc
     6361 cggccgaggc agccgccgcg ccggcggagg aagcgccgcc cacggaagcc gcgcccgggg
     6421 aagcgccggc gccggtggag gaaacgcctg ctcccgagga agcgcctgtc cccgaagaga
     6481 ccccagcgcc cgaggaagcg ccgcccgccg cggaagcccc ggcgccggag gcgcccgccg
     6541 tccccgagat ggacccggtg ctgcgcgaca tcttcacccg cgaggccagc gggcatctcg
     6601 aggtgctgcg cgagtacatc gccggctgcg ccctgcgcac cgcgccctat gccgtgaccg
     6661 aggccgcgca ccgcgcctcg cacaccctcg ccggcagcgc caacatggcc gacgtcgcgc
     6721 ccgccgtggc ggtggcgcgg ccgctgaacg agcacctgcg ccgcctgtac gacgatcacg
     6781 tcggcctgtc ggaggccggc ctcgagctgg tgcgccgtgc cgtggacacc attgccggcg
     6841 tggtcgaggc gctcacggaa ggccgcgcgc cggaggccgc cgactatgac ctggccgccg
     6901 agatccgcgc gctgcacgag gactaccagc tgcgcgcgga ggccggggcc acggtgacgc
     6961 tgcccgagta ccggccgccc gaggagttcg atcccgagat cctggcgctg ttctgcgagg
     7021 aagcgaccga gattctcgag gaagcggagg gcgcgatggc gtcctggcgc gcgcgcccga
     7081 gctccgagga accggtgcgc gcgctgcagc gctacctgca caccatcaag ggcagcgcgc
     7141 ggctcgccgg cgtgcgcacc atcggcgatt tcagccatga cctcgagaac ctgttcgagg
     7201 cactcgccga cggcagccgg gtcagcgagc ccgcgttggt cgagatcgcc cagcagagcc
     7261 tcgacgccat gcacggcatg cgcgacgtgg ccgccgcggg acacatgccc gagattccgg
     7321 ccggcctgcg cgagcgcatc gccgccgcgc tcaagggcga ggaaatcacg ccgtacgtgc
     7381 cgcccgaaga ggaagtggcg ccggtcgagg aagaagcgcc ggccgaggcc gcgccgccgg
     7441 cagaagcgcc cgcggcgccg gtcgaggaag aagtgcccgc cgcgccggcc gaggagccca
     7501 cggcccccgt cgagcaagaa gcacccgcgc cggtctacgt gccgccggcc gagtacccgc
     7561 cgccgctgat cacgttcccg ccggagcgcg cgccggagcc cgagccaacg cccgagccaa
     7621 cgcccgagcc gacgcccgag ccgacgcccg aggccgagcc cgcggtcacg ccggagccga
     7681 cgccggagcc gatgccggag ccgaagccgg agccgaagcc ggagccgaag cccgaaccgc
     7741 tgccgccgct ggcgcccgag gtgccggcgc tgcgcgccgg acgcgcggcc cgccaggaag
     7801 tggcgcgggt cgacgccgag ctgctcgaga acctgctcaa cagcgcgggc gaggtcggca
     7861 tctaccgctc gcgcctcgag gagcagctcg gctcggtgca gttcaacctc gaggagctcg
     7921 gcgccacggt ggtgcgcctg cgcgaccagc tgcgcaagat ggagatcgag accgagacgc
     7981 agatcatcca tcgccaccag ggcgacatgg acgcgcgcgg cgatttcgat ccgctcgagc
     8041 tggaccgcta ttccatgatc cagcagctgt cgcgcgcgct cgccgagacg gccagcgacg
     8101 tcggcagcat ccagcagctc atcggcgagc gcatgcgcga ggccgagacc atcatcgtgc
     8161 agcagtcgcg cgccgtcagc gagctgcagg acggcctgat gcgcacccgc ctggtgccct
     8221 tcaaccgcca cgcgcagcgg ctctcgcggc tggtgcgcca ggtggcctcc gagtacggcc
     8281 ggcgcgccga gctgcaggtg gtcggcggca ccgccgagct cgaccgccag atcatggatc
     8341 gcatgctcgg gccgatggaa cacctgctgc gcaatgccgt catccacggc atcgagccgc
     8401 cggaggcgcg cctggccgcc ggcaagtccg acaccggccg gatcaccgtc ggcgtgcgcc
     8461 gcgaaggcgc cgaggtggtg gtggaagtgg gcgacgacgg ccgcggcctg gacgtgcccg
     8521 ccatccgcca gaaggcgttg aagctggcgc tcatcaagcg cgacgatccc ttcggcgagg
     8581 ccgaggccgc cgaggtcatc ctgcggcccg gcttcagcac cgcgaccgag ctcacccagg
     8641 gcgccgggcg cggcgtcggt accgacgtgg tggccagcga agtgcgccag ctcggcggct
     8701 cgctggaagt gcgcaaccgg gcgggccagg gcgtcacctt cgagatccgc ctgccgttca
     8761 cccgcgccat cacccaggcg ctggtgctgc gcgccggcga agagtggtac gccttgccgc
     8821 tgccggcggt
//
LOCUS       DAOMPG010000091         8804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3416_length_8804_cov_4.451709, whole genome shotgun sequence.
ACCESSION   DAOMPG010000091 DAOMPG010000000
VERSION     DAOMPG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_8804_cov_4.451709"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1332
                     /locus_tag="PLS34_06490"
     CDS             1..1332
                     /locus_tag="PLS34_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRP87150.1"
                     /translation="MLFVAGGVALGLLLGLLALQPRTSRLRARLAVAESRLEARALAD
                     AEREATLALAEQRLGRVFDNLARRSLQDNSRNFLELARQNLEVHRSRAQGDLKAREQA
                     VEALVAPIREALHKTERQIAAIEKDRHEAFGNIRGLLESISLSQQALSTETRNLVTAL
                     RRPEVRGQWGEMTLRRLVELAGMVEHCDFTEQPHTTSEDGGVMRPDMIVHLAEGRQLV
                     VDVKTPLDAYLAAVEAPDDAAREAALQRHARKVRERVRELAAKAYWSQFEHSPEFVIL
                     FIPGDQFLSAALARDPGLLDEALNQHVILATPTSFVALLKAVAYGWRQVALAENARRI
                     RDLASDLYRRLAAFTAHLSRVGRSLAGSVDAYNSAVGSLERQVLPGARRFTELGVQAR
                     RPLDELPEVEAVARSVSGSDRPATDAVDTPDTTAAVDPPAPEGGGREPSAT"
     gene            1329..2180
                     /locus_tag="PLS34_06495"
     CDS             1329..2180
                     /locus_tag="PLS34_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRP87151.1"
                     /translation="MSALPDPGSRSAPPGSPYWYAVLFTPAASRVAVGALLDLRAELR
                     DAARRPSEPAVARARLEWWRAELAGFGGGREQHPATRLLSRVSPEGVVQVEYLLELLD
                     AAETERAAAPCRNYAELSLYCYRSSGVLHEMLAGLLGLAEPGNERAVRRHAQRLGTGI
                     RLVEIIANLQGDLAAGRRLLPRDWIMETGASEEPAADGRMEVALATCLDRLAGEARLA
                     LDEADALLPSAERSHQRTGLVLAALHRRRLAKLHAGGFDPRALPGNLDNLWTAWRAAR
                     RAGQTHS"
     gene            2195..2950
                     /locus_tag="PLS34_06500"
     CDS             2195..2950
                     /locus_tag="PLS34_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HRP87152.1"
                     /translation="MDVERITSYEAAPGLLTDRVILVTGAGAGIGRALARAAAGLGAT
                     VILLGRRVRPLEFVYDEICAAGGPTPAIFPLNLERATGEDYDRLSGAIEEQWGRLDGL
                     VHNAGILGSRTPIEHHDVGEWQRVLHVNLTAPFILCRCLLPLLLKSEDASVIFTSSGV
                     GRRGQAYWGAYAVSKFGVEGLSQVLADELANKPAIRVNAVNPGATRTSMRAAAYPAED
                     PQKLKTPEEILGAYLYLLGPASRGVTGRSFDAQ"
     gene            complement(2955..3806)
                     /locus_tag="PLS34_06505"
     CDS             complement(2955..3806)
                     /locus_tag="PLS34_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRP87153.1"
                     /translation="MKKRPTLTRRKTTPAAEAEPVRIHKLLADAGIGSRREIERWIAE
                     GRIAVNGRPAEVGERLTGSEKIAIDGRPVRINEPSRHRYLAYHKPADEVCTRRDPEGR
                     RTVFDSLPTLRGQRWIVVGRLDVSTTGLLLFTTDGALANALMHPSGEVLREYAVRVLG
                     EPDESTIARLLAGVELEDGVASFESVVPAGGDGRNRWFHVTLREGRNREVRRLWEAVG
                     LTVSRLTRVAYGPVTLDRQLSRGRFRDLDAEEVRALYACVGRPAPKLAAVDARALKPR
                     RRRSAPS"
     gene            complement(3757..4407)
                     /gene="scpB"
                     /locus_tag="PLS34_06510"
     CDS             complement(3757..4407)
                     /gene="scpB"
                     /locus_tag="PLS34_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRP87154.1"
                     /translation="MDPEKLKNIVEAALLAAGRPLGIEQLQALFGEHERPDRAAFKAA
                     IESLEADYLGRPIRLLEVASGWRIQVREEMAPWLSRLWQDRPARYSRALLETLALIAY
                     RQPITRGEIEDIRGVAVSSNIMRTLMERGWVRIVGHRDVPGRPGMYATTREFLDYFGL
                     KRLDELPPLAEIRDLDSINVELDLGLTEPPSVPSTEEAEEHEEATDTDASQDHARG"
     gene            complement(4421..5257)
                     /locus_tag="PLS34_06515"
     CDS             complement(4421..5257)
                     /locus_tag="PLS34_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRP87155.1"
                     /translation="MGSGQAEMPFAIVDGEPLTQVPMDLYIPPHALEVILDAFEGPLD
                     LLLYLIKRQNIDILNIPIAKITHQYMQYIEVMQELQLELAAEYLVMAAMLAEIKSRML
                     LPRPASQEEEGEDPRAELVRRLQEYERFKKAAEDVDALPRLERDVFQAGAEMCERKVV
                     EILPDVTLRELLLSFQEVLSRADMFAHHHVQRETLSVRQRMADVLERIQGGQFVEFTA
                     LFTPEEGRMGVVVTFLAILELIRETLLDIVQAEPFAPIHVKSRGGEHAPSNDTTQPAA
                     GA"
     gene            complement(5326..6543)
                     /locus_tag="PLS34_06520"
     CDS             complement(5326..6543)
                     /locus_tag="PLS34_06520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRP87156.1"
                     /translation="MSSTTGPNRRVLSGMRPTGALHLGHLHGALKNWLRMQYEYDCFF
                     FVADWHALTTHYEDPSGIAPSVREMLVEWLAVGINPAAATVFVQSDVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKERDLGTYGFLGYPLLQSADILIYRAGYVPVGEDQVA
                     HVEITREIARRFNHLYGREADFEQRAERAVRHLGGKLSKLYLEQRRSYQERGDEAALE
                     TGRALVENHPRLTLADRERLLGYLEGIGRTILVEPDALLTPTPRVPGLDGQKMSKSYG
                     NTIGLAEAPEAVEKKLRTMQTDPARVRRTDPGEPEKCPVWQFHRIYSDENTQDWVQKG
                     CRSAGIGCLECKQPVIDAVLAELQPIRDKIAEYKASPATVDIILSEGRERARDAARET
                     MDDVREAMGLEYR"
     gene            complement(6571..7215)
                     /locus_tag="PLS34_06525"
     CDS             complement(6571..7215)
                     /locus_tag="PLS34_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRP87157.1"
                     /translation="MQQLSLLQLLAVAAIPVLFAITLHEVAHGWTARQFGDRTAEMLG
                     RLTANPLKHIDPIGTVVVPALALLTTGFLFGWAKPVPVATRNLRNPRRDMMFVAVAGP
                     AANILMALFWAFFTKLATLSGLQGMTLQFFLTMGQIGVFINVILAVFNMLPIPPLDGG
                     RVLSGLLPPRASTRLDRIEPYGLIIVLAFIFFGWRYFQPVITALRDFFFSLAGL"
     gene            complement(7249..7875)
                     /locus_tag="PLS34_06530"
     CDS             complement(7249..7875)
                     /locus_tag="PLS34_06530"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRP87158.1"
                     /translation="MAELIQIHPKNPQARLVREVAELVEGGGIVAYPTDSSYAFGWAM
                     GNKAAQERIRRLKHLDKHHHFTLVCENLSELSTYARVDNQAYRLLKAFTPGPYTFILP
                     ATRQVPNRLQHPKRKSIGIRVPNHAIALAILAELPEPLMSTTLQLPDQEHPMNDPWEI
                     QERLDHELAAIVDGGPCALEPTSVIDLTGQVPVVTRAGLGDVSHLDAA"
     gene            7914..8186
                     /locus_tag="PLS34_06535"
     CDS             7914..8186
                     /locus_tag="PLS34_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRP87159.1"
                     /translation="MNEQRIEMIRERLLDALEPEMLEIEDESHLHAGHAGARDGRGHF
                     RVTLVSEAFSGHSPIRRHRLVYAALGGLMETDIHALTIQAYAPGEV"
BASE COUNT         1441 a         3019 c         2891 g         1453 t
ORIGIN      
        1 gtgctgttcg tcgccggcgg cgtcgcgctc ggcctgctgc tcggcctgct ggcactgcag
       61 ccacgcacca gccggctgcg cgcgcggctc gccgtcgcgg aaagccgcct ggaggcgcgc
      121 gcactcgccg acgccgagcg tgaggccacg ctggcgctgg ccgagcaacg cctgggccgc
      181 gtgttcgaca acctggcgcg ccgctcgctg caggacaaca gccgcaactt cctggagctg
      241 gcgcggcaga acctcgaagt gcaccgcagc cgcgcccagg gcgacctcaa ggcgcgcgag
      301 caggcggtcg aggcgctggt cgcgccaatc cgcgaagcgc tgcacaagac cgagcggcag
      361 atcgccgcca tcgagaagga ccgccacgag gccttcggca acatccgcgg actgctcgaa
      421 tccatcagcc tcagccagca ggccctgagc accgagaccc gcaacctggt gaccgccctg
      481 cgccgccccg aggtgcgcgg ccagtggggc gagatgacgc tgcgccgcct ggtcgaactg
      541 gcgggcatgg tggagcactg tgatttcact gaacagccgc acaccacgag cgaggacggc
      601 ggcgtgatgc gcccggacat gatcgtccac ctggccgagg gccggcagct ggtcgtggac
      661 gtgaagacgc cgctcgacgc ctacctggca gcggtggagg cgccggacga tgccgcccgc
      721 gaggcggcgc tccagcgcca cgcccgcaag gtccgggagc gggttcgcga gctggccgcc
      781 aaggcctact ggtcgcagtt cgagcacagc ccggagttcg tcatcctgtt cattccgggc
      841 gaccagttcc tcagcgcggc gctggcgcgc gatcccggcc tgttggacga ggcgctgaac
      901 cagcacgtca ttctcgccac gcccaccagt ttcgtcgcgc tgctcaaggc ggtggcctac
      961 ggctggcggc aggtcgcgct ggcggagaat gcccggcgga ttcgcgacct ggccagcgac
     1021 ctgtaccgcc gactggcggc cttcaccgcg cacctgtcac gggtgggtcg ttcgctggcg
     1081 ggcagcgtgg acgcctacaa cagcgccgtc ggctcgctcg agcgccaggt cctgcctggc
     1141 gcgcggcgct tcaccgagct cggcgtgcag gcccgccggc cgctggacga gttgcccgag
     1201 gtcgaggccg tggcccgctc ggtgtccggc tccgaccggc cggcgacgga cgcggtcgac
     1261 acgccggaca cgacggcagc ggtcgacccg cccgcacccg agggcggcgg ccgcgagccg
     1321 tcggccacgt gagcgcgctg ccggacccgg gctccaggtc ggcgccgccg gggtcgccct
     1381 attggtacgc ggtgctgttc acacccgctg cgtcccgcgt ggcagtcggc gcgctgctgg
     1441 atcttcgcgc cgagctccgc gatgcggcgc ggcggccatc ggagccggcc gtggcccgtg
     1501 cacggctgga gtggtggcgg gccgagctcg ccggcttcgg cggcgggcgc gagcagcatc
     1561 cggcgacgcg gctgttgagc cgagtctccc ccgagggcgt ggtgcaggtg gaatacctgc
     1621 tcgagctgct cgacgcagcg gagaccgagc gcgccgcagc gccgtgccgg aactacgcgg
     1681 agctgtcctt gtactgctat cgcagcagcg gtgtgctgca cgaaatgctg gccgggctgc
     1741 tcggcctggc cgagcctggc aacgagcgcg ccgtccggcg ccacgcgcag cgactcggca
     1801 ccggcatccg gctggtggag atcattgcga acctgcaagg cgacctggcc gccggccggc
     1861 ggctgctgcc gcgcgactgg atcatggaaa ccggcgccag cgaggagccg gcggcggacg
     1921 gccgcatgga ggtcgccctt gcgacctgcc tcgatcgtct cgccggtgaa gcacgcctgg
     1981 cgctcgacga agccgacgca ctgctgccgt ctgccgagcg gtcgcaccag cgcaccggac
     2041 tggtcctcgc ggcgcttcac cggcgccgcc tcgcgaagct gcatgccggc ggcttcgacc
     2101 cgcgggcgct gcccggcaac ctggacaatc tctggaccgc gtggcgcgct gcgcggcggg
     2161 ccggacaaac gcacagctga ggggagacac gcgcatggac gtcgagcgga taaccagtta
     2221 cgaggccgcg cccggcctgc tgaccgaccg ggtgatcctg gttaccggcg ccggcgccgg
     2281 catcggacgc gcacttgcga gggcagccgc gggccttggt gcgaccgtca ttctgctggg
     2341 acggagagtg cgtccgctcg aattcgtgta cgacgagatc tgtgccgctg gcggcccgac
     2401 gccggcgatc tttccactca acctggagcg ggccaccggc gaggactacg atcgcctgag
     2461 cggtgctatc gaggagcagt ggggacggct cgacgggctg gtgcacaacg ccggcatcct
     2521 cggcagccgt acgcccatcg agcaccatga cgtcggtgaa tggcagcgcg tgctgcacgt
     2581 caacctgacg gcgcccttca ttctctgccg ctgcctgctg ccgctgctgc tcaagtccga
     2641 ggacgcgtcg gtgattttca cctccagcgg cgtgggccgt cgtggccagg cctactgggg
     2701 cgcctacgcc gtgtccaagt tcggcgtcga agggctcagc caggtgcttg ccgacgagtt
     2761 ggcgaacaag cccgccatcc gggtcaacgc agtgaacccg ggcgcgaccc ggacgtcgat
     2821 gcgcgccgcg gcctatcccg ccgaggaccc gcagaagttg aaaacgcccg aggaaatcct
     2881 cggcgcctac ttgtacctgc tcggccccgc cagccgcggt gtcaccggcc gcagcttcga
     2941 cgcacagtag ccgatcagct cggcgccgag cgacggcgcc gcggtttcag cgcgcgggca
     3001 tcgaccgccg caagcttcgg cgccggtcgc ccgacacagg cgtacagcgc acgcacctcc
     3061 tcggcgtcca ggtcccggaa ccgtccacgg ctgagctggc gatccagcgt cacgggcccg
     3121 taagcgacgc gcgtgaggcg gctgacggtc aggcccaccg cttcccagag cctgcgcacc
     3181 tcgcgattgc ggccttcacg cagcgtgacg tgaaaccagc ggttgcgccc gtcaccacca
     3241 gccggcacga ccgattcgaa cgacgccacc ccatcctcga gctccacgcc ggccagcagg
     3301 cgcgcgatgg tcgactcgtc cggctcaccg agcacccgca cggcatattc gcgcagcact
     3361 tcgccggacg gatgcatcag cgcgttcgcc agcgcgccat ccgtggtgaa caacaacagg
     3421 ccggtggtgc tcacgtccag ccggcccacc acgatccagc gctggccgcg cagcgtcggc
     3481 agcgagtcga acaccgtgcg gcgcccctcc ggatcgcgcc gcgtgcagac ctcgtccgcc
     3541 ggcttgtggt aggcgagata acgatggcgc gacggttcat tgatgcgcac cggccggccg
     3601 tcgatggcga tcttttcgct acccgtcagg cgttcgccga cttccgccgg gcgaccgttg
     3661 acggcgatgc ggccttcggc gatccagcgc tcgatctcac gccgcgaacc gatgccggcg
     3721 tccgccagca gcttgtgaat cctgaccggc tccgcctcag ccgcgggcgt ggtcttgcga
     3781 cgcgtcagtg tcggtcgctt cttcatgctc ttcggcctcc tccgttgacg gcacggatgg
     3841 cggttccgtg agcccgagat caagctcgac gttgatgctg tcgaggtcgc ggatctccgc
     3901 gagcggcggc agttcatcga gccgcttcag tccgaaatag tcgagaaact cgcgggtggt
     3961 ggcgtacatc cccggccggc ccggcacgtc gcggtggccc acaatccgca cccagccgcg
     4021 ctccatgagg gtgcgcatga tgttggagct cacggccacg ccgcgaatat cctcgatctc
     4081 gccgcgagtg atcggctggc gataggcgat cagcgccagg gtctcgagca gggcgcggga
     4141 ataccgggcc gggcggtcct gccagagccg cgacagccac ggcgccattt cctcgcgcac
     4201 ctggatgcgc catccgctcg ccacctcgag gaggcggatc ggccgaccca ggtagtccgc
     4261 ctcgagcgat tcgatggcgg ccttgaaggc tgcccgatcc ggccgttcat gctcgccgaa
     4321 cagggcctgc agctgctcga tgcccagcgg ccggcccgcg gccagcagcg ccgcctcgac
     4381 aatgttcttc agtttttcag gatccatcgt ggtttccgct tcaggcgccg gcggccggct
     4441 gcgtggtgtc gttggacggt gcgtgctcgc cgccgcgcga cttgacgtga atcggcgcga
     4501 aaggctccgc ctggacgatg tcgagcaggg tctcgcgaat cagctccagg atcgccagga
     4561 aagtgaccac cacgcccatg cggccctctt ccggcgtgaa cagcgcagtg aactcgacga
     4621 actggccgcc ctggatccgc tccagtacgt cggccatccg ttgtcgcacc gagagcgtct
     4681 cacgctgcac gtggtggtgc gcgaacatgt ccgcccgcga cagcacttcc tggaagctca
     4741 gtaacagctc ccgcagcgtg acgtccggga ggatctccac gaccttgcgc tcgcacatct
     4801 cggcgccggc ctgaaatacg tcccgctcga gccgcggcag cgcatcgacg tcctcggccg
     4861 ccttcttgaa gcgctcgtat tcctgcagcc ggcgcaccag ctcggcgcgt ggatcctcgc
     4921 cttcttcctc ctgcgaggcc gggcgcggca gcagcatgcg cgacttgatc tccgccagca
     4981 tcgcggccat caccaggtat tccgccgcga gctcgagctg cagttcctgc atcacctcga
     5041 tgtactgcat gtactggtgc gtgatcttcg cgatgggaat gttgagaatg tcgatgttct
     5101 gccgcttgat caggtaaagc agcaggtcca gcggtccctc gaatgcgtcg aggatgacct
     5161 cgagcgcgtg cggcgggatg tagaggtcca tcggcacctg ggtgagcggc tcgccgtcca
     5221 cgatcgcgaa cggcatttcc gcctggcccg agcccatcgg cggctcgatc gcgccgctcg
     5281 tctcggctgc gtcgccggcc tcgaccgcca ctgacgattc tccgctcatc ggtactcgag
     5341 ccccattgcc tcgcgaacgt cgtccatggt ctcacgcgcc gcgtcgcgcg cccgctcgcg
     5401 accctccgag agaatgatat cgaccgtcgc ggggctggcc ttgtactccg cgatcttgtc
     5461 gcgaatcggc tgcaactccg ccagcacggc gtcgatgacc ggctgcttgc actcgaggca
     5521 gccgatgccg gcgctgcggc agcccttctg cacccagtcc tgcgtatttt cgtccgaata
     5581 aatgcggtga aactgccaga ccgggcattt ctccggctcg ccgggatccg tgcggcgcac
     5641 ccgcgcggga tcggtctgca tggtgcggag ctttttctcc actgcctccg gcgcctcggc
     5701 aaggccgatg gtgttgccgt aagacttgga cattttctgg ccgtcgagtc ccggcacccg
     5761 cggcgtcggc gtcagcagcg cgtccggctc caccaggatg gtgcggccga tgccctcgag
     5821 atagccgagc aggcgctcgc gatcggccag cgtcaagcgc ggatggttct ccaccaacgc
     5881 cctgccggtt tccagcgccg cctcgtcgcc acgctcctgg tagctgcgcc gctgctcgag
     5941 atacagcttg ctcaacttgc cgccgagatg ccggactgca cgttccgcgc gctgctcgaa
     6001 atcggcctcg cggccgtaga gatggttgaa gcgccgcgca atctcgcgcg tgatttcgac
     6061 atgcgccacc tggtcctcgc cgaccggcac atagccggcg cggtagatga ggatgtcggc
     6121 gctctgcagc aaggggtagc cgagaaagcc gtaggtgccc aggtcgcgtt ccttgagctt
     6181 ctcctgctgg tccttgtagg tgggtaccct ttccagccag cccagcggcg tgatcatgga
     6241 gagcaacagg tgcaactcgg cgtgctctgg cacgtccgac tgcacaaaca ccgtcgccgc
     6301 ggccggattg atgccgaccg ccagccactc cacgagcatc tcgcgcacgc tcggcgcgat
     6361 gcctgacggg tcctcgtagt gagtcgtcaa agcatgccag tcggcgacga agaaaaagca
     6421 gtcgtactcg tactgcatcc gcagccagtt cttcagggca ccgtgcaggt ggccgagatg
     6481 caatgcgccg gtgggcctca tgcccgacag gacgcgccga ttaggaccgg tagtgctgct
     6541 caatgctcgc tccgtgcaga gatgctgtga tcacagtccc gccaacgaga agaagaagtc
     6601 tcgcagcgcc gtaatcaccg gctgaaaata cctccatccg aaaaagatga aggccaggac
     6661 aattatgagg ccataaggct cgatccggtc gagccgggtc gaggcacgcg gcggcagcag
     6721 gccgctcaat acccgaccgc cgtccagcgg cgggatcggc aacatattga acactgccag
     6781 gatcacgttg atgaacacac cgatctggcc catggtcagg aaaaactgca aggtcatgcc
     6841 ctgcaggccg ctgagcgtcg ccagcttggt gaaaaaagcc cagaaaagcg ccatcaggat
     6901 attggcggcg ggtcccgcca cggcgacgaa catcatgtcg cgacgcggat tcctgagatt
     6961 gcgggtcgcc accggcacgg gcttggccca gccgaacagg aacccagtgg tcagcagcgc
     7021 aagcgccggc acgaccacgg tgcctatcgg gtcgatgtgc ttgagcggat tggccgtcag
     7081 gcgccccagc atctcggccg tgcgatcccc gaattgccgc gccgtccagc cgtgcgccac
     7141 ttcgtggagg gtgatggcaa acaaaaccgg aatcgcggcc acggccagga gttggagcaa
     7201 ggagagctgc tgcatcgggc tattataggg aagcggacgc cggctgagtc aggccgcgtc
     7261 cagatggctg acgtcgccga gtcccgcccg tgtcactacc ggaacctggc cggtcaaatc
     7321 gatcaccgag gtcggctcca gcgcgcacgg cccgccgtcg acgatcgccg caagctcgtg
     7381 gtcgagccgc tcctggatct cccagggatc attcatcggg tgctcctggt cgggcagctg
     7441 cagcgtcgtg ctcatcagcg gctccggcag ctcggccagg atggccagcg cgatcgcgtg
     7501 gttcggcacg cggatcccga tgctcttgcg cttcgggtgt tgcaagcgat ttggcacctg
     7561 ccgagtggcc ggcaggataa atgtgtacgg gccgggggtg aaggccttga gcaggcgata
     7621 cgcctggttg tcgacccgcg cgtacgtgga caactccgac aggttctcgc agacgagtgt
     7681 gaaatgatga tgcttgtcca ggtgcttcag gcggcgaatt cgctcctggg cggccttgtt
     7741 gcccatcgcc cagccgaaag cgtaggaaga atccgtcggg tacgcgacaa tgccaccgcc
     7801 ctcgacgagt tcggccacct cccgcaccag ccgcgcctgc ggattcttcg ggtgtatttg
     7861 gatcaactcc gccatgggcg gcattctatc tgagtaaaac cggggtcaga gcaatgaatg
     7921 aacagcgcat cgagatgatc agggaacgac tcctcgatgc cctcgagccc gagatgctgg
     7981 agatcgagga cgagagtcac ctccacgctg gccacgcggg cgcccgggac gggcgtggtc
     8041 atttccgggt cacgctggtg tcggaggctt tttcaggcca ctccccgatc cgccgccacc
     8101 gcctggttta cgcggccttg ggcggcctga tggagactga catccatgca ttgaccatcc
     8161 aggcctatgc tcccggagag gtctgagggc accgcgcaca tcacgatgct gcaacgcatc
     8221 aatgtcaggt cctgcggaaa accgcagcct ccctggacca attccggcag tgaaaactgt
     8281 gatgcaatcc agaatgttgc ccaccgaact gtgagtggct tcacagtttg acttaatcca
     8341 cacctggtat agcgcgccgc cctcgcgaag ccgatcccgg tgacggcccc cgagaccttc
     8401 ctgatctggg caaacattga tcaaccgtcc gggctgcccc ggcaagctct gcatggacct
     8461 gacaaacgca tattctgtca aattgacaga attctggcgc gaaattctgc gatctgggga
     8521 ccagctggct ggaaagccac cccttcgccc gctcctcctt ttcacactgg ccaggtctga
     8581 tccccgagca gcctggtcgt ctgagattgt tttaacataa cctggcggtg tcaagttgct
     8641 cgacggtctg tgtcggccat gagatatccg ttgttactct ctgagtttgg tcgatttttg
     8701 acctcccctc ttgcggccag gtccgatcga agatcacaga agcagccata ttgcacgcca
     8761 caatatgttg aataattgac cggaaatttg ggctggttat agtc
//
LOCUS       DAOMPG010000092         8764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3437_length_8764_cov_4.059708, whole genome shotgun sequence.
ACCESSION   DAOMPG010000092 DAOMPG010000000
VERSION     DAOMPG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_8764_cov_4.059708"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLS34_06540"
     CDS             complement(<1..831)
                     /locus_tag="PLS34_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP87160.1"
                     /translation="MKQALLEFALRRPRTVYLLALLLTVVLGLQIPRIQIDTDPENML
                     PADQADRVFHNEVKEEMGLHDAIVVGLVEPDKVDGIYNPASLGALYRATERILELDGV
                     VAQDLMALSVADNIGQAGPGTVRFEWLMPEAPRTAEAALAIRSHVERLPLLKDTLASG
                     DGRAAAIYVPILDKNQSHRLSREIQAITAAASVEFGATGQWHITGLPVAEDTFGYEMF
                     VQMGISAPLAGLAIFLLLLMFFRSLPLIVAPMIVAMVTVIATMGLLIGMGFTVHIMSS
                     M"
     gene            1084..1323
                     /locus_tag="PLS34_06545"
     CDS             1084..1323
                     /locus_tag="PLS34_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRP87161.1"
                     /translation="MNERDLEAAVLRALEHAAGDVGDRPIEPERPLAEQFTLDEERFL
                     QALARETGLDIPPQDCIELRTLSDCMKYLAGGWSD"
     gene            1370..1813
                     /locus_tag="PLS34_06550"
     CDS             1370..1813
                     /locus_tag="PLS34_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRP87162.1"
                     /translation="MRHVARLARWLDTALRIPGTRIRFGLDALLGIVPGLGDALGALI
                     GGYIVLAALRAGAPRLVIVRMLANIGVDAIVGAVPVAGTIFDVAYKANRRNAELLAEW
                     AQHPVAVAGRQRRLALAIVLALLGLLAIASTALAFGAWAVIRLLG"
     gene            1885..3006
                     /locus_tag="PLS34_06555"
     CDS             1885..3006
                     /locus_tag="PLS34_06555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP87163.1"
                     /translation="MSRAVGIAGPSELAVRAGAAAAAAGGNAVDAAISAALAALCTET
                     AIVGLDAAAYVTVWHPDQPPVVIDGGIVMPGLGQPALRFGAAGRPVSLDYGGGMETVV
                     GYGAVGVGGAVAALGEAAAGWSSLPWDALVATAADQVRDGFPLSRASHTWLQYSHREI
                     FGWQAESAAVIHDEDGALLPAGATIRVPGLVDTLETLGREGPGAFYGGRLGDQIAACV
                     QGGGGLLGRRDRRAYRAQRREPTHCQVDGWSIAVPPPPAVGGTALAAMLARMGGTPAA
                     AWDAAAVARLATVQREVFEFLRTLPFVDPARIGIWGDRCRAGIAARLGAQRRRPGRPP
                     LTVDGARFGAGPGRTRLRDYRILRLRVGGTRAAHRHLAE"
     gene            2900..3442
                     /locus_tag="PLS34_06560"
     CDS             2900..3442
                     /locus_tag="PLS34_06560"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP87164.1"
                     /translation="MHVSALDQDGLGCAITASSGYGSGVLVPRTGIWLNNCLGELELN
                     PRGFHGLQPGQHMASNMSPTVARGVDGAVLVLGSPGAERITTALLQVMLNFMRLGMSL
                     EEALAAPRLHVERVVDAWQAAAEPGLPLDAVPMPWRQFDALSMFFGGAGAVLREADGT
                     LIAAADPRRQGAAVVTHTTD"
     gene            complement(3486..4019)
                     /locus_tag="PLS34_06565"
     CDS             complement(3486..4019)
                     /locus_tag="PLS34_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002806616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRP87165.1"
                     /translation="MATAASWLARLESAEVRFCLRANRSCDRAPVLALFSTVSRLGDG
                     IIWYGLMLALPLLYGTAGAAVTVQMALAGLLGTAVYRWLKERLVRERPCNLHPRIRAG
                     ARPLDRYSFPSGHTLHAVSFTLIATAAFPALVVLLLPFTVLVAMSRVILGLHYPSDVA
                     AGALLGYLLARLALVVA"
     gene            complement(4090..5136)
                     /locus_tag="PLS34_06570"
     CDS             complement(4090..5136)
                     /locus_tag="PLS34_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009670503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRP87166.1"
                     /translation="MAGQTITIGDVSIAPGERRGLSLPVGMLHTHVPVEMPVWVINGR
                     RAGPRLFISAAIHGDELNGIEIIRRIRMRPLAGLRGTLLLVPVVNVFGLLHHARNLPD
                     RRDLNRSFPGSERGSLAARLAHQFMTEIVDRCTHGIDLHTGAIHRSNLPQIRAQLDDP
                     ETRELATAFGAPVVLDSRLRDGSLREAAAEKGIPTLIYEAGEALRFDELSIRMGVAGI
                     VAVMRKLDMLRGKPRRARPARPVIANGSHWVRAPEGGILRTAVRLGQRVRAGELLGQV
                     SDPFGAHDVPVTALHAGIVIGRNSLPLVNEGDALFHIARVERPGEAFAAVEAIAGAFR
                     DPPVDNGGNGVMSP"
     gene            complement(5150..6055)
                     /gene="rimK"
                     /locus_tag="PLS34_06575"
     CDS             complement(5150..6055)
                     /gene="rimK"
                     /locus_tag="PLS34_06575"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HRP87167.1"
                     /translation="MHIAILSRNRQLYSTRRLIEAARQRGHEVSIVDPLKCVMDVTSL
                     RPAVYHRGERLPRFDAVIPRIGASVTFYALAVLRQFEVMGVYPANESVAIGRSRDKLR
                     SLQLLARAGIGMPVTAFAHSPIDTKTLVRLVGGAPLVLKLLEGTQGRGIVLAETNKAA
                     ESVIDAFRELDANFLVQQFIREAGGADIRAFVVGDRVVAAMLRQAKEGEFRSNLHRGG
                     SASIVKLSPAERATAVRAARTIGLNIAGVDIVRGKDGPLVLEVNSSPGLEGIETTTGK
                     DVAGMIMEFIEKKARPHKTRTRGKG"
     gene            complement(6055..6528)
                     /locus_tag="PLS34_06580"
     CDS             complement(6055..6528)
                     /locus_tag="PLS34_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HRP87168.1"
                     /translation="MAAKSPRTTLGWREWVGLPELGIPRIKAKVDTGARTSALHAFEV
                     LPFERDGVRWVRFSVHPDQYDRSKIVQCESPVLDRRMVSDSGGHREQRWVIESTVAVG
                     ELSWRIEMTLTDRDPMRFRMLLGRTALRGRFIVDPARSYCLGRTRRTRAAFREKN"
     gene            complement(6532..7617)
                     /gene="corA"
                     /locus_tag="PLS34_06585"
     CDS             complement(6532..7617)
                     /gene="corA"
                     /locus_tag="PLS34_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRP87169.1"
                     /translation="MARNRGVRRRSQPGSPPGHPIESVAADVGGTTVAWRRYNETHLD
                     EQADIGLAAALDGLDPAVVDWVHFSAAPDPAALRWLHERLGLDPLALEDVHNGHQRTK
                     LELYDKHSFMVVSVPVLQDGELTLEQFSLFLGPHWVISFWSGPPALVEPIRQRLLANG
                     GSGRIRKRGADYLLYCLLDAAVDTAFPVLETLRDVIEALEEDLLARPNEESIARIHEL
                     RRKLALMRRLVLPGQEALSRLLRSDDSPLSHATRRYVHDVLDHHLRIGDTIDNLVEVT
                     RSLHELYLLSISHRMNDVMKLLTIIATIFIPLTFIAGVYGMNFDPDVSPWNMPELRTR
                     FGYPLVMLLFAGIGVGMAWMFRRRGWF"
     gene            7699..8553
                     /locus_tag="PLS34_06590"
     CDS             7699..8553
                     /locus_tag="PLS34_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRP87170.1"
                     /translation="MACLFVEQLTVLDFSRLDRERGLVGESWIVDLELEGALDAQAMV
                     LDFGDVKRIARDAVEALADHKLLAPVQCGWLEVVESPASGEIRVEAPAAGLRLRSPGV
                     AVYRLAAAEVTMPALRPVLERCVAAEMPANVSGVRVALREESVPGAYYHYSHGLKRHA
                     GNCQRIAHGHRSRIEIYTDGRRNDHWELAWAERWRDIYLGSEEDVVAETRLGDRPAWR
                     FGYAAEQGEFELEIWQDRCELVPADTTVEELAGYVAASLARLDPGTRFRVRAYEGVHK
                     GAIAEAGG"
     gene            8658..>8764
                     /locus_tag="PLS34_06595"
     CDS             8658..>8764
                     /locus_tag="PLS34_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRP87171.1"
                     /translation="MKRVLLFLATNIAVLVVLSIVLSLIGFEGIMDAQGV"
BASE COUNT         1340 a         2938 c         3133 g         1353 t
ORIGIN      
        1 catggagctc atgatgtgca cggtgaagcc catgccgatc agcaggccca tggtcgcgat
       61 caccgtgacc atcgcgacga tcatgggcgc gacgatcagc ggcaggctgc ggaagaacat
      121 cagcagcaac aggaaaatcg ccagcccggc gagcggcgcg gaaatgccca tctgcacgaa
      181 catctcgtag ccgaaagtgt cctccgccac cggcaggccg gtgatgtgcc actggccggt
      241 ggcgccgaat tccaccgacg cggccgccgt gatcgcctgg atctccctcg acaggcggtg
      301 gctctggttc ttgtccagga tcggcacgta aatcgcggcc gcgcggccgt cgcccgaggc
      361 cagcgtgtcc ttgagcagcg gcaggcgctc cacgtggctg cgaatggcga gcgccgcctc
      421 ggcggtcctt ggggcctcgg gcatgagcca ctcgaagcgc accgtgcccg gcccggcctg
      481 gccgatgttg tcggccaccg acagggccat caggtcctgc gccaccacgc cgtccagctc
      541 gaggatgcgc tcggtcgctc gatacagcgc gcccagcgag gcggggttgt agatgccgtc
      601 aaccttatcc ggctcgacca gtcccacgac gatcgcgtcg tggagcccca tctcctcctt
      661 gacctcgttg tggaacacgc ggtcggcctg gtcggccggc agcatgttct ccgggtcggt
      721 gtcgatctgg atccgcggga tctgcagccc gagcaccacc gtcagcagca gcgcgagcag
      781 gtagaccgtc cgcgggcgac gcagcgcaaa ttccagcaat gcctgtttca attccgtgcc
      841 tccctgggga cccgccggcg ccggtgcgcc gtgcggggcg ccaaactctg atggccgagg
      901 atattaattt agaaactact aatatgcaac cccgccaggc accctaaaga tggttgccgg
      961 gccagccttc ggccttgacc tgcatcaagc ggagctaaac ttgccgatgg caagctggca
     1021 acgccgcgtg gcgaagcgcg cggtccgcag tggccggccg acggcaagtc gaggaggtgg
     1081 ccgatgaacg agcgcgatct tgaagcggcg gtgctgcggg ccctggagca tgccgcaggc
     1141 gacgtcggag accgcccgat cgagccggag cggccgctgg cagagcagtt cacgctggac
     1201 gaggaacggt ttttacaggc gctggcgcgc gagaccggcc tggacattcc cccgcaggac
     1261 tgcatcgaat tgcgcacgct gtccgactgc atgaaatacc tcgccggcgg gtggtccgat
     1321 taaggccggc gatgccggcg gcaccgagat ctccggtgcg actcaccgac tgcgtcatgt
     1381 agctcgactg gcgcggtggc tggacaccgc gctgagaata cccggcaccc ggatccgttt
     1441 cgggctcgat gccctgctcg gcatcgtgcc ggggctgggc gatgccctcg gtgcgctgat
     1501 cggcggctac atcgtgctgg ccgcgctgcg cgccggcgcg ccgcgcctgg tcatcgtgcg
     1561 catgctcgcg aacatcggcg tggacgccat cgtcggcgcc gtgcccgtcg ccggaaccat
     1621 tttcgacgtc gcctacaagg ccaaccgtcg caacgccgag ctgctggccg aatgggcgca
     1681 gcacccggta gccgtggcgg gccgccagcg ccgcctggcg ctggcgatcg tgctggcgct
     1741 gctcggcctg ctggccatcg cttcgacggc gctggccttc ggcgcctggg cagtaattcg
     1801 cctgctgggt tagattgagg cgcgtcaggg cagcaggaac gttgagccgg ccatgttgag
     1861 cgagcatttc gggacccggg aggaatgagc cgggcggtcg ggatcgcagg accgtcggaa
     1921 ctcgccgtgc gcgccggcgc cgctgcggcc gcggccggcg gcaatgccgt cgatgcggcg
     1981 atctccgccg ccctggcggc gttgtgtacc gagaccgcga tcgtcgggct ggatgcggcc
     2041 gcctacgtca ccgtctggca cccagaccag ccgcccgtgg tcatcgacgg tggcatcgtc
     2101 atgccggggc tcggccagcc ggcactgcgc ttcggtgcgg cgggacggcc ggtcagcctc
     2161 gactacggcg gcggcatgga gacggtcgtg ggctacggtg cggtgggcgt gggcggtgcc
     2221 gtcgcggcgc tcggcgaagc tgcggcgggc tggagcagcc tgccctggga cgcgctggtc
     2281 gccacggcgg cggaccaggt tcgcgacggg tttccattgt cacgcgcttc ccacacctgg
     2341 ctgcagtaca gccaccgcga aatcttcggc tggcaagccg agagcgccgc cgtcattcat
     2401 gacgaggatg gcgccctgct gccggccggg gcgaccatcc gggtgccggg cctggtggac
     2461 acgctggaga cgctggggcg tgaaggtccc ggggcctttt atggcggccg gctcggcgac
     2521 cagattgccg cctgcgtgca gggcggcggc gggctgctcg gtcgccgcga ccggcgcgcc
     2581 taccgggcgc agcgacgcga accgacacat tgccaggtgg acggctggtc gatcgcagtc
     2641 ccgccgccgc cggccgtcgg tggcaccgcc ctggcggcaa tgctggcgcg catggggggc
     2701 accccggcgg ccgcctggga cgctgctgca gtggccaggc tggctaccgt ccagcgtgag
     2761 gtgttcgaat tcctgcgcac gctgccgttc gtcgatccgg cgcgcatcgg catctggggc
     2821 gaccgatgcc gcgctggaat cgctgctcgc ctgggcgcgc agcggcggcg acccgggcgg
     2881 ccgccgctca ccgtcgacgg tgcacgtttc ggcgctggac caggacggac tcggctgcgc
     2941 gattaccgca tcctccggct acgggtcggg ggtactcgtg ccgcgcaccg gcatctggct
     3001 gaataactgt ctcggcgagc tggagctgaa cccgcgcgga tttcacggcc tgcagccggg
     3061 ccagcacatg gcctcgaaca tgtcgccgac ggtggcgcgc ggcgtggacg gcgcggtgct
     3121 cgtgctgggc agcccgggag cggagcgaat caccaccgcg ctcctgcagg tgatgctcaa
     3181 cttcatgcgc ctcgggatgt cgctggagga agcgctggcc gccccgcgcc tgcacgtcga
     3241 gcgcgtggtg gacgcctggc aggccgccgc cgagccgggc ttgccgctcg acgccgtgcc
     3301 catgccctgg cggcagttcg acgccctgtc gatgttcttc ggcggcgccg gggccgtgct
     3361 gcgcgaggcc gacggcacgc tcatcgcggc ggcggatccg cgccgccagg gggctgcggt
     3421 ggtgacgcac acgactgact gacggtgcgc ggatgtccgt gggccacgcc cggccgtcgt
     3481 gtcgctcagg ccaccacgag cgccagccgc gccagcaggt agccgagcag ggcgccggct
     3541 gcgacgtcgc tggggtagtg cagcccgaga atcacccgcg acatggccac cagtacggtg
     3601 aagggcagca gcaggacgac cagtgccggg aaggcagccg tggcgatgag ggtgaacgac
     3661 accgcgtgca gcgtatgccc ggagggaaaa ctgtagcggt cgagcggccg cgcgccggca
     3721 cggatgcggg gatgcaggtt gcagggccgt tcgcgcacca ggcgctcttt cagccagcga
     3781 tagaccgcgg tgccgagcag gcctgccagc gccatctgca cggtgactgc cgcgccggcc
     3841 gtgccgtaca gcaacggcag ggcgagcatc agcccgtacc agataatgcc gtcgcccagc
     3901 cggctgacgg tcgagaacag cgccaggacc ggggcccggt cacaggagcg attggcccgc
     3961 aggcagaacc ggacctcggc actctcgagt cgcgcaagcc agctggcggc ggtcgccata
     4021 tgccatgacc tcagaccttg gctagccgga atcctggcag cacttggcta cggccgggtt
     4081 aaggcggcgc tacggggaca tgacgccgtt gccgccgttg tccacgggcg ggtcgcggaa
     4141 cgcgccggcg atggcctcga ccgcggcgaa ggcctcgccc ggtcgctcga cgcgcgcgat
     4201 gtgaaacagc gcgtcgccct cgttgaccag cggcaggctg ttgcgcccga tgacaatacc
     4261 ggcatgcagc gcggtgaccg gtacatcgtg cgcgccgaag gggtcggaca cctgtccgag
     4321 cagctcgccg gcccggacgc gctgcccgag ccggaccgcg gtgcgcagga tgccgccctc
     4381 tggagcgcgc acccagtgac tgccgttcgc gatcaccggc ctggcgggtc gcgcccggcg
     4441 cggcttgccg cgcagcatgt ccagcttgcg catcacggcg acgatgccgg cgacgcccat
     4501 gcgtatggac aactcgtcga agcgcagcgc ctcgccggct tcgtagatca gcgtcggaat
     4561 ccccttctcg gcggccgcct cgcgcagcga cccgtcgcgc agccgggagt cgagcacgac
     4621 cggcgcgccg aaggccgtcg ccagctcgcg ggtctcggga tcgtcgagct gtgcgcgaat
     4681 ctgcggcagg ttgctgcgat ggatggcgcc ggtgtgcagg tcgatgccat gggtgcaacg
     4741 gtcgacgatc tcggtcatga actggtgcgc cagccgcgcg gccagggaac cgcgttcgga
     4801 gccggggaaa ctgcggttga ggtcgcgccg atccggcagg ttacgggcgt gatgcagcag
     4861 cccgaacaca ttcacgaccg gcaccagcag cagcgtgccg cgcagcccgg ccagcggccg
     4921 catgcggatc cgccggatga tctcgatgcc gttgagttcg tcgccgtgga tcgccgcgct
     4981 gatgaacagc cgcggcccgg cgcgacgtcc attgatcacc cacaccggca tttccaccgg
     5041 gacgtgcgtg tgcagcatgc cgaccggcag cgacaagccg cggcgctcgc cgggcgcgat
     5101 gctgacgtcg cctatggtga tggtttgtcc agccatcgga tcctcgcctt caacccttgc
     5161 cgcgggtccg ggtcttgtgg ggacgggcct tcttttcgat gaattccatg atcatccccg
     5221 cgacgtcctt gccggtggtc gtctcgatgc cttccagccc cggcgaggag ttgacctcca
     5281 gcaccagcgg gccgtccttg ccgcgcacga tgtcgacgcc ggcgatgttc agcccgatgg
     5341 tcctggcggc gcgcactgcg gtggcacgct ctgcggggct cagcttgacg atcgaggcgg
     5401 aaccgccccg atgcaggttc gagcggaact cgccttcctt ggcctggcgc agcatcgccg
     5461 ccaccacccg gtcaccgacg acaaaggcac ggatgtcggc accgccggcc tcgcggatga
     5521 actgctggac caggaaattg gcgtccagct cgcgaaatgc atcgatgacg ctttccgccg
     5581 ccttgttggt ctcggcgagc acgatgccgc ggccctgggt gccctccagc agcttgagca
     5641 ccagcggcgc gccgcccacc agccgtacca gcgtcttggt gtcgatcggc gagtgcgcga
     5701 aggcggtcac cggcatgccg atgccggcgc gcgccagcag ctgcaaggag cgcagcttgt
     5761 cgcggctgcg gccgatggcc accgactcgt tcgccgggta gacgcccatc acctcgaact
     5821 ggcgcaagac cgccagcgcg tagaacgtaa ccgaggcgcc gatacgcgga atcacggcat
     5881 cgaagcgcgg caggcgttcg ccgcggtggt acaccgccgg ccgcagcgat gtgacgtcca
     5941 tgacgcactt caacgggtcc acgatgctga cttcgtggcc gcgctgcctg gcggcttcga
     6001 tcaagcggcg ggtggaatag agctgccgat tcctcgacag gatcgcgatg tgcattaatt
     6061 cttctcccga aaggcggcac gcgtccggcg cgtgcggccc aggcagtagg acctggccgg
     6121 atccacgatg aagcgtcccc gcagggcggt gcgaccgagc aacatgcgaa agcgcatcgg
     6181 gtctcgatcg gtcagggtca tctcgatgcg ccagctcagc tcaccgaccg cgaccgtgct
     6241 ctcgatcacc cagcgctgtt cgcgatgccc gccagagtcc gagaccatgc gccggtcgag
     6301 caccggtgac tcgcactgga cgatcttgct gcgatcgtac tggtcggggt gcacgctgaa
     6361 gcgcacccag cggacgccgt cgcgctcgaa cggcagcact tcgaaggcgt gcagggccga
     6421 ggtgcgcgcg ccggtatcca ccttggcctt gatgcgcggt atgccgagtt ccggcagccc
     6481 gacccattcg cgccagccaa gcgtggtgcg gggagatttt gccgccatgc ctcagaacca
     6541 gccgcgacgg cgaaacatcc aggccatgcc gacgccgatg cctgcgaaca acaacatcac
     6601 cagcggatag ccgaagcgcg tgcgcagctc gggcatgttc cacgggctga cgtccggatc
     6661 gaaattcatg ccgtacacgc cggcaatgaa agtcagcggg atgaagatcg tggcgatgat
     6721 ggtgagcagc ttcatgacgt cgttcatgcg atggctgatg ctcaacaggt acagctcgtg
     6781 caggctgcgg gtcacctcca ccaggttgtc gatggtgtcg ccgatgcgca ggtggtgatc
     6841 cagcacgtcg tggacatagc gccgcgtggc gtggctgagc ggggagtcgt cgctgcgcag
     6901 caggcggctg agggcttcct gtccgggcag taccaggcgg cgcatcagcg cgagcttgcg
     6961 ccgtaactcg tgaatcctgg cgatcgactc ctcgttcggc cgcgcgagca gatcctcctc
     7021 gagcgcctcg attacgtcac gcagcgtttc cagcacgggg aaggcggtgt cgaccgccgc
     7081 gtcgagcagg cagtaaagca agtagtcagc gccgcgcttg cggatgcgac cgctgccgcc
     7141 gttggccagc agccgctggc ggatgggctc caccagtgcc ggagggccgc tccagaaact
     7201 gatcacccag tggggaccga ggaacaggct gaactgctcc agcgtcagct cgccgtcctg
     7261 caggacgggg accgagacca ccatgaagct gtgcttgtcg tacagctcca gcttggtgcg
     7321 ctggtggccg ttgtgcacgt cctccagcgc cagcggatcc aggccgagcc gctcgtgcag
     7381 ccagcgcaag gcagccgggt cgggggctgc tgagaaatgc acccagtcga ccaccgccgg
     7441 atcgaggccg tcgagcgccg cggccaggcc gatgtcggcc tgttcatcga ggtgcgtctc
     7501 gttgtaacgc cgccacgcga ccgtggtgcc gccgacgtcg gcggccaccg actcgatcgg
     7561 gtgccccggc gggctgccgg gctggctcct gcgccgcacc cccctgttcc tggccatacg
     7621 cttttcccct gcggtcaccc gcgcatcata gcgcgtcgca gtacaatcat cgctggcctt
     7681 gccgagggtg ccggtccgat ggcctgcctg ttcgtcgaac aactgacggt gctggatttc
     7741 tcgcggctgg accgcgagcg cgggctggtg ggcgagagct ggatcgtcga tctcgagctg
     7801 gaaggtgcgc tcgacgccca ggccatggtg ctcgatttcg gcgacgtcaa gcgcatcgcg
     7861 cgggatgcgg tcgaggcgct ggccgaccac aagctgctgg cgccggtcca gtgcggctgg
     7921 ctggaggtcg tcgagtctcc cgccagcggc gagatccgcg tcgaggcgcc ggccgccggg
     7981 ctgcggctgc gctccccggg cgttgcggtg taccggctgg cggctgcgga agtcaccatg
     8041 ccggcgttgc ggccggtgct ggagcgctgt gtcgcggcgg aaatgcccgc caacgtcagc
     8101 ggggtgcggg tggccttgcg cgaggaaagc gtgccgggcg cctattacca ctacagtcac
     8161 ggcttgaagc gccacgccgg caactgccag cgcatcgcgc atggccaccg ttcgcgcatc
     8221 gagatttaca ctgacgggcg gcgcaacgac cactgggagc tcgcctgggc cgagcgctgg
     8281 cgcgacatct acctggggtc ggaggaagac gtggtggcgg agacccgact gggcgatcgg
     8341 ccggcctggc ggtttggcta cgcggcggag cagggcgagt tcgagctgga aatctggcag
     8401 gatcgctgtg aactggtccc ggcggacacg acggtggagg agctcgccgg gtacgttgcc
     8461 gccagcctag cacgcctgga tccgggcacc cggttccggg tacgggccta cgagggggtg
     8521 cacaagggcg cgattgccga ggcgggcggc tgacgagacc cgggaacccg ccgccccgcc
     8581 cgagggtctt ggaaatgggc ggttgtggcc caacatagcg tgtagcgccg ggggtccgga
     8641 gcgaaggaga gtctttgatg aagcgcgtgc tgctgttcct ggcgaccaac atcgccgtgc
     8701 tggtggtttt gtccatcgtc ctcagcctga tcggcttcga ggggatcatg gacgcgcagg
     8761 gggt
//
LOCUS       DAOMPG010000093         8657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3498_length_8657_cov_4.462451, whole genome shotgun sequence.
ACCESSION   DAOMPG010000093 DAOMPG010000000
VERSION     DAOMPG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_8657_cov_4.462451"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLS34_06600"
     CDS             <1..606
                     /locus_tag="PLS34_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87172.1"
                     /translation="ASNGSGQTARAPLSFDDQDSRITAFAALEATAPWGPLTQRLFAV
                     TWMPDTLATDGLGAPARDDYFALTAKLFAEWPIGAGGMRGGIGGEAGYAPNTPVESVV
                     NVGAGAGADGLAHQFSFNLFDFAPGHNIAFVYGRAGAGWLISPDFRNNDRLQEVRYQW
                     KFSKNWSMETRLRRRDEIDVPTGTPQIRVDDDFYIRLTGRF"
     gene            623..1561
                     /locus_tag="PLS34_06605"
     CDS             623..1561
                     /locus_tag="PLS34_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HRP87173.1"
                     /translation="MDPIVLFFILGLAAGLARSDLRLPGPIYEFLSVILLLAIGLKGG
                     VELAQQSLAALLPQMIAVVIMGSLLPLAAYPVLRFAGRLPRVDAAAIAAHYGSVSVGT
                     YAVAVAWLVSRNIRYEEHMPLLLVMLEVPAIVVGIMLARGAAAGTDWKRLGHEVFLGK
                     SIVLLIGGLLIGWAAGPEALEPVSGLFFGLFHGVLALFLLEMGLVAAGQVGSLRRHGL
                     FLVAFGVLFPLFGAVIGGALGLLLSLSPGGIMLLATLAASASYIAVPAALRLALPEAN
                     PTLSLTASLGVTFPFNILVGIPLYMRLADLLHATGG"
     gene            1566..1868
                     /locus_tag="PLS34_06610"
     CDS             1566..1868
                     /locus_tag="PLS34_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRP87174.1"
                     /translation="MRTEARTLVTIVTEAALERRITDDIRRLGAHGYTVSDARGEGSR
                     GVRSAGWDATSNIRIEVVCDDRTAEAIAAHLRAHYYDHYAMILFMSEVGVLRPEKF"
     gene            complement(1885..2400)
                     /locus_tag="PLS34_06615"
     CDS             complement(1885..2400)
                     /locus_tag="PLS34_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRP87175.1"
                     /translation="MSTRLMRGLLLASLLLVLAAATAAVRSYERTLGEGLIMLAELAP
                     VDPRSLMQGDYMALRFAIDDELGTRGADPATGRLPRYAWLSRDAEGRASLDSLADDLP
                     PADDERLAVRIRRRGGQPSIGPNAFFFQEGTAEHYETARWGEFRVDRRGRALLTHLRD
                     EELRRLGEIDR"
     gene            complement(2397..3449)
                     /locus_tag="PLS34_06620"
     CDS             complement(2397..3449)
                     /locus_tag="PLS34_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HRP87176.1"
                     /translation="MTVGSPTGLLDRLVEVGVLEAERAAELAAAPSTPWWLAALQGFA
                     AWVASFLIMSSFFAPLMFLGEGPVGRGIAGAALLAAAVWLFRRGHAFTAQMALAFSLA
                     GQALVVGAFAGDFDASVNFRMLSLVGLLVAAGMFVAPATQLHRVVCALLMTGHLGYLI
                     GPGNPLALYAVALAAAAVAAWLVRRRWPARRAQTIRALVHAVTLGALVVAVIVGTQAA
                     RNLGISPGATPVLNPVIYPAGIGLLLLGAVTWLGRHESSAFRLGSLGGALLFVVAAWR
                     APGLMASAAVLLAVFHAGHRAWAALALLAAMIYLGEFYYSLHATLLAKSGALAATGLV
                     LLGLRLGLGMWDRGRP"
     gene            complement(3446..4423)
                     /locus_tag="PLS34_06625"
     CDS             complement(3446..4423)
                     /locus_tag="PLS34_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRP87177.1"
                     /translation="MAWSVGEVGQLLKLAEDGALSPAELARAEQVGALVPSPAAWRRA
                     ADRLLAFGGMLLLAAAVVFFFAYNWADLHRFWKLGMAALALTACTGAALFAAPAGSTY
                     RATLMGACICTGVLLALVGQIYQTGADVWELFAAWALLMLPFVLLARSSASWALWLAV
                     ANLALGRALSQSAWSGWLGAHFARDSMLLVAAANLAVLVLFEARGERLLAVPRRHLQR
                     LAALGMLMPLAIGGMMGWWEDEFVPVTGAFLLVAGGAAWFYLKWRRDLVILALALFGA
                     IPVFTSALVRALPKESDFFALNLVALFVIGSSALAGKWLVGLSREGRGE"
     gene            complement(4549..5745)
                     /locus_tag="PLS34_06630"
     CDS             complement(4549..5745)
                     /locus_tag="PLS34_06630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRP87178.1"
                     /translation="MALRRKLYAGRDFRRALSIEELRGIARRRLPHFAFEYIESGAED
                     ESTLRWNRAIFEQLRFLPRTLVDTTARNQRTKLLGRDSHAPLVIAPTAMNGMLHRRGD
                     TALARAAAAAGIPSCLSTFSNVRLEALAAEVQGRLWMQLYLLGDRAIARDIVGRAEQA
                     GYEALVLTTDANVFGFREWDRRNYRAPGRLTLRNLFDAARRPRWALDVMVPDGFPCFE
                     NVREFLPPEAQSARAGVAHLPRLLVPDVSWADVEGLRRAWPRKLLVKGILTVEDARRA
                     VDAGCDGIVLSNHGGRQLDGCISPLETLPEVVAAVGEHAAVLVDSGFRRGTDIVKALA
                     LGADAVMVGAATLYGLAAGGEEGARHAIGLLTGEIDRVLGQLGCNSVQELGPQLLTGG
                     RDRPAG"
     gene            complement(5787..6233)
                     /locus_tag="PLS34_06635"
     CDS             complement(5787..6233)
                     /locus_tag="PLS34_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87179.1"
                     /translation="MFMIRLLVFSILLPAVATGCAARYQPPAPGDRHALLALPSQEAQ
                     LDRGMFLEPVALNGLTRPRNWLVESFRMPPGQFRLLARAAQDAVQGSCLLQFDAVAGE
                     TYLLDAERVDGMFTLSVSHGGEVVAECSAPATVLPTPARIPGAPAR"
     gene            6308..6949
                     /locus_tag="PLS34_06640"
     CDS             6308..6949
                     /locus_tag="PLS34_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRP87180.1"
                     /translation="MNTWQLKVHNDGRDTRVRLVHDSGLCRGWSGDLACRLCRHLGLS
                     DGEHPCPKQQAWRLFLAAHALEHALPEPRMNPPDARQVLLEHGLTPTPARTAIAAMLL
                     PHKRHVTADSVASELALLGRKLSLARVRLTLREFVEWDLLRAIDVGGGVVFYDPVIAP
                     HAHVYNVDTGELTDLSPEQAWITGLPELPDDVRLDDVQLVFRVRSKSGEPSAI"
     gene            complement(6938..7453)
                     /locus_tag="PLS34_06645"
     CDS             complement(6938..7453)
                     /locus_tag="PLS34_06645"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRP87181.1"
                     /translation="MTERYYLAYGSNLHPLRLAQRVPSARLVGTTPLPGYRLAFHKRG
                     MDGSAKCDLELTDDSGSLAYGAVYSLATPDIARLDRLEDLGTGYFKEQVTLRVDGVAL
                     SAFVYFASHTHVAPDLLPFDWYLGFVLAGARRHGFPAEYVAQVAAVPHQTDPNEGRRR
                     GMLELLERLDG"
     gene            complement(7561..>8657)
                     /locus_tag="PLS34_06650"
     CDS             complement(7561..>8657)
                     /locus_tag="PLS34_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87182.1"
                     /translation="RGMREEMQKSGLPGGLGDMLMTAPPDQPWLSANPNDMTQMYGMM
                     LRGAADGKEEQARRDARAVDAAHERAQIAARTRLVGQTTVDGRPALHLLAADVNHRQV
                     EDGVEITIRDVNMFVDAERYVPLSLRMDGTMREGGDTRDITIERHDQEYRQVPGCGRM
                     YRPFKTVTRIAGVMNAEQEAQMREAQLQMAEMDKQLAQMPPGQRDMIMKQMGPQMEMM
                     RNMAAGGGIEVVSNVRDMRCNVPVPNPMTLARSPFGGGTPGMPASMGAAMPVQPSAGA
                     THAPASAEAARQACLQEKVAAAQAAQKKKRGFGKLLNAAARTASRFGNQDVGQIASDV
                     HAAGATADDLAGAARDLGLTEDEIAECDNP"
BASE COUNT         1401 a         3156 c         2823 g         1277 t
ORIGIN      
        1 gccagcaatg gctccgggca gactgcgcgc gcgccgttgt cattcgacga ccaggacagc
       61 cgcatcaccg ctttcgctgc actcgaggct accgcgccct gggggccgct cacccagcgc
      121 ttgtttgccg tgacctggat gccggacacg ctggcgaccg acggcctcgg cgcgcccgcg
      181 cgtgacgact atttcgcact gacggcaaag ctgttcgccg agtggccgat cggcgccggc
      241 ggcatgcgcg gcggcatcgg gggcgaagcg ggctatgcac cgaacacgcc cgtcgagtcg
      301 gtcgtgaatg tgggcgccgg cgccggcgcc gacggcctgg cgcaccagtt ctccttcaac
      361 ctgttcgact tcgcccccgg gcacaacatc gctttcgtct acggccgcgc cggtgccggc
      421 tggctgatct ctccggactt ccgcaacaac gatcgtcttc aggaggtccg gtaccaatgg
      481 aagttctcga agaactggtc catggagacc cgcctgcggc gccgcgacga gatcgacgtt
      541 ccgaccggca cgccacagat acgcgtcgat gacgatttct acattcgcct gaccgggcgt
      601 ttctagccgc acggcagcag cgatggaccc gatcgtcctg ttcttcatcc ttgggctggc
      661 cgccggcctc gcccgctccg acctgcggct ccccggtccg atctacgagt tcctgagcgt
      721 catcctgctg ctcgcgatcg ggctgaaggg cggcgtcgag ctggcgcagc agtcgctggc
      781 cgccctgctg ccgcagatga ttgccgtggt catcatgggc agcctgcttc ccttggcggc
      841 gtatccggtg cttcgtttcg ccgggcgcct gccacgtgtc gacgccgcgg ccatcgccgc
      901 gcactacggc tcagtcagcg ttggtaccta tgccgtcgcg gtggcgtggc tggtgtcgcg
      961 aaacatccgc tacgaggagc acatgccgct gctgctcgtc atgctggaag tgccggccat
     1021 cgtcgtcggc atcatgctgg cacgcggagc tgccgcgggc accgactgga agcggctggg
     1081 gcacgaggtg tttctcggca agagcatcgt gctcttgatc ggcggcctgc tgatcggctg
     1141 ggcggcgggc ccggaggccc tggagcccgt gtccggcctg ttcttcggac tgttccacgg
     1201 cgtgctggcg ctgttcctgc tggagatggg cctggtggcg gccgggcagg taggcagcct
     1261 gcggcgccac ggcctgttcc tggtggcctt cggggtgctg ttcccgctgt tcggcgcagt
     1321 gatcggcggc gcgctgggcc tgctgctgag cctgtcgccc ggcggcatca tgctgctggc
     1381 cacgctggcg gcgagcgcct cttacattgc agtgcccgcg gcgctgcgcc tcgcgctgcc
     1441 cgaggccaat ccgacgctct cgttgacggc gtccctgggc gtgaccttcc ccttcaacat
     1501 cctggtcggc atcccgctgt acatgcggct ggccgatctc ctgcatgcca ccggaggctg
     1561 aggccatgcg aaccgaagcc cgcacgctcg tcaccatcgt caccgaggcg gccctcgagc
     1621 gccgcatcac tgatgacatc cggcgcctgg gcgcgcacgg ctacacggtc agcgacgccc
     1681 gcggcgaagg cagccgcggc gtgcgcagcg ctggctggga cgcgaccagc aatatccgca
     1741 tcgaagtggt ctgtgacgac cgcactgccg aggcgatcgc cgcacatctg cgggcccact
     1801 actacgatca ctacgccatg atcctgttca tgagcgaagt cggggtgctg cggcccgaga
     1861 aattctgaaa gcggcaaggc cggctcagcg gtcaatttcg ccgagccgcc ggagctcctc
     1921 gtcacgcagg tgggtgagga gcgcgcggcc gcggcgatcc acccgaaatt caccccagcg
     1981 cgcagtctcg taatgctccg cagtgccctc ctggaagaag aacgcgttgg gcccgatgct
     2041 cggctggccg ccgcggcgcc ggatccgcac ggccagccgc tcatcatccg ccgggggcag
     2101 gtcatcagcc agcgagtcca gcgacgcccg gccctcggca tccctgctca accaggcgta
     2161 gcgcggcagc cggcccgttg caggatctgc gccgcgcgtg cccaactcgt cgtcgatggc
     2221 gaaacgcagc gccatgtaat ccccctgcat gagcgagcgg gggtccaccg gtgcgagttc
     2281 ggccagcatg atgaggccct cgccgagggt acgctcgtag ctgcgcaccg ccgcggttgc
     2341 cgccgccagc actagcagca atgacgccag cagcagccct cgcatcaacc gggtgctcat
     2401 ggccgtcccc tgtcccacat gccgagtccc agccgcaggc ccagcagcac caggccggtc
     2461 gccgccagcg cgccggactt cgccagcagt gtggcatgca ggctgtaata gaactcgccg
     2521 agataaatca tggcggcgag cagcgccagt gcggcccacg cccggtgacc cgcatgaaag
     2581 acggccagca gcacggccgc cgaggccatc agcccgggtg cccgccacgc cgccaccacg
     2641 aacagcagcg caccgccgag cgaccccagc cggaatgcag aggattcatg ccgcccgagc
     2701 caggtcaccg cacccagcag cagcaggccg atgccggccg gatagatcac ggggttgagg
     2761 accggcgtgg cgccagggct gatacccagg ttgcgagcgg cctgtgtgcc tacgatcacg
     2821 gcgaccacca gcgcgcccag ggtgacggca tggaccagcg ccctgatcgt ctgtgcacgc
     2881 cgggcgggcc agcgcctgcg caccagccag gcagcgacgg ccgctgccgc gagcgcgacg
     2941 gcatacagcg cgagcggatt gcccgggccg atcaagtatc ccaggtggcc ggtcatcagc
     3001 agcgcgcaca cgacacggtg cagctgcgtg gccggcgcga cgaacatgcc tgcggcgacc
     3061 agcaggccga ccagcgacag catcctgaag ttcaccgagg catcgaaatc gccggcgaac
     3121 gcgccgacca ccagggcctg gccggcgagc gaaaaagcca gcgccatctg tgccgtgaac
     3181 gcatggccgc ggcgaaacag ccacactgcc gcagccagca gggccgcgcc ggcgatgccg
     3241 cgacccaccg gcccctcacc gaggaacatc agcggcgcga agaaggaaga catgatcagg
     3301 aacgacgcca cccacgcggc gaatccctgc agggcggcga gccaccaggg cgtcgacggg
     3361 gccgccgcca gctccgcggc gcgctccgct tccagaacgc cgacctcgac cagccggtcc
     3421 aggagaccgg ttggcgaccc gaccgtcatt ccccgcgacc ctcgcgactg aggccgacca
     3481 gccacttgcc ggccagggcc gaactgccga tcacgaacag ggcgacgagg ttcaaggcaa
     3541 agaagtcact ctctttcggc agcgcgcgca ccagcgcgct ggtgaacacc ggaatggcgc
     3601 cgaacaacgc cagcgccagg atgaccaggt cgcgccgcca cttgaggtag aaccacgcgg
     3661 cgccgcccgc gacgagcagg aaggcgccgg ttaccgggac gaactcgtct tcccaccagc
     3721 ccatcatgcc gccgatcgcc agtggcatca gcattccgag cgcggccagc cgctgcaggt
     3781 gccggcgcgg caccgccagc aggcgctcgc cccgtgcctc gaacagcacc agcaccgcca
     3841 ggttggcggc agcgaccagc agcatcgagt ccctggcgaa atgggcgccg agccagcccg
     3901 accaggccga ctgcgacaag gccctgccga gcgccagatt ggccaccgcc agccacagcg
     3961 cccagctcgc cgaagatcgc gccagcagca cgaagggcag catcagcaag gcccaggccg
     4021 cgaacagttc ccacacgtcg gcaccggtct ggtagatctg tccgaccagc gccagcagca
     4081 cgccggtgca gatgcaagcc cccatcagcg tcgcccggta cgtgctgccc gccggtgccg
     4141 cgaacagcgc ggcgcccgta caggccgtca gcgccagcgc ggccatcccg agcttccaga
     4201 agcgatgaag gtccgcccag ttgtaggcaa aaaagaacac cactgccgcg gcgagcaaca
     4261 gcatgccgcc gaaggccagc agccggtcgg ccgcccgccg ccaggcggca ggcgacggga
     4321 ccagtgcacc gacctgctcg gcccgcgcca gctcggccgg ggacagcgcg ccgtcctccg
     4381 ccagcttcag cagttgcccg acctcaccca ccgaccaggc catgttctca cctcggagcg
     4441 ccacggcgcc gtggcgacct gttcccgcgt gacgctgcgg gcagtatggt aggctgcccg
     4501 cggactgtcg agagcaaggc caggcgtcgg caagccggtg ttgattcgct agccggccgg
     4561 ccgatcgcgc ccgcccgtca acagctgtgg accgagttcc tgcaccgagt tgcagccgag
     4621 ctggccgagc acccggtcga tctcgccggt cagcaaaccg atggcgtgcc gtgcgccctc
     4681 ctcgccgccc gccgcgagtc catagagcgt cgccgcgccg accatcacgg cgtctgcgcc
     4741 aagggccagc gccttgacga tgtccgtgcc gcggcggaaa ccgctgtcca ccagcaccgc
     4801 cgcgtgctca ccgaccgccg cgacaacctc cggcagcgtc tccagcggcg agatgcagcc
     4861 gtcgagctgg cgcccgccgt ggttgctcag cacgatgccg tcacagccgg cgtccactgc
     4921 gcgcctcgca tcctccacgg tcaggattcc cttgaccagc agcttgcgcg gccaggcccg
     4981 ccgcagcccc tcgacgtccg cccagctcac gtccggaacc agcaggcgcg gcaggtgcgc
     5041 tacaccggca cgcgcggact gcgcctcggg cggcaggaat tcgcgaacat tctcgaaaca
     5101 cgggaagccg tccggcacca tcacgtccag cgcccagcgc ggccggcgtg ccgcatcgaa
     5161 caggttgcgc agcgtcagcc ggccgggcgc gcggtaattg cgccggtccc actcccggaa
     5221 gccgaacacg ttggcgtcgg tggtcagcac cagtgcttcg tatccggcct gctcggcccg
     5281 accgacgatg tcgcgggcga tggcgcgatc cccgagcagg tagagctgca tccacaggcg
     5341 gccctgcacc tcggccgcca gtgcttccag ccgcacgttg gagaaggtgc tgaggcacga
     5401 gggaatcccg gccgcggccg cggcgcgtgc cagggccgtg tcgccgcgcc gatgcagcat
     5461 gccgttcatc gcggtgggcg cgatcaccag cggcgcgtgg ctgtcacgac ccagcagctt
     5521 cgtccgctgg ttgcgcgccg tcgtgtcgac gagcgtgcgg ggcaggaatc gcagctgctc
     5581 gaaaatcgcg cgattccagc gcagcgtcga ctcgtcctcg gcgccggact cgatgtactc
     5641 gaaggcaaaa tgcggcagcc ggcgccgcgc gatgccgcgc agttcctcga tcgacagggc
     5701 acggcggaaa tcccggccgg catacagctt gcgacgcaat gccatgcagg catcttaacc
     5761 gcgacctggc ggctgatgcg ggccggtcaa cgcgctggcg cgccgggaat cctcgccggc
     5821 gtcggcagca ccgtggccgg cgcgctgcat tccgccacga cttcgccgcc gtgagacacg
     5881 ctcagggtga acatgccgtc gacccgctcc gcatcgagca ggtaggtttc tccagccacg
     5941 gcgtcgaact gcagcaggca gcttccctgc acggcgtcct gcgcggcccg cgccaacagc
     6001 cggaactggc ccggcggcat gcggaagctc tccaccagcc agttgcgcgg cctggtcagc
     6061 ccgttcaagg ccacgggctc gagaaacatc cctcgatcga gctgcgcctc ttgtgacggc
     6121 agcgccagca gggcgtgccg gtctcccggc gcgggtggct ggtaccgggc cgcacaaccg
     6181 gtggcgactg caggcaacag gatggagaac accagcaagc ggatcatgaa catggtggcg
     6241 tcctgttgaa atgcgaatga ttcctattta cactcaagtc gcaatcgaag gtcctgggag
     6301 cgagcctatg aacacatggc aactgaaagt gcacaacgat ggccgagaca cgcgggtccg
     6361 cctggtgcac gattcgggct tgtgccgcgg ctggagcggg gacctggcct gccggctgtg
     6421 ccgtcacctg ggcctcagcg acggcgaaca cccctgtccc aagcagcagg catggcggct
     6481 cttcctggcg gcccacgcgc tcgagcacgc cctgcccgaa ccgcgcatga atccgcccga
     6541 tgcccggcag gtgctgctcg agcacggcct cacgccgaca ccggcgcgca ctgcgatcgc
     6601 ggccatgctc ctgccccaca agcgccacgt caccgccgat tcggtggcgt ccgaactcgc
     6661 cctgctgggc cggaaacttt ccctggcgag agtaaggctg accctgcgtg agttcgtgga
     6721 atgggacctg ttgcgcgcca tcgacgtcgg cggcggcgtg gtcttctacg acccggtcat
     6781 cgcgccacat gcccatgtct acaacgtcga taccggggaa ctcaccgatc tctcgcccga
     6841 acaggcctgg atcaccgggc tgcctgagtt gccggacgat gtgcggctcg acgatgtgca
     6901 actggtgttc cgggtgcgat cgaaatcggg cgaaccttca gccatctagg cgctccagca
     6961 gctccagcat gccgcggcgc cgcccctcgt tcgggtccgt ctggtgtggc actgcggcaa
     7021 cctgtgcaac atattccgcc ggaaagccgt gtcgccgggc gccggcgagc acgaagccca
     7081 ggtaccagtc gaacggcagc aaatccgggg cgacgtgcgt gtggctggca aaatagacga
     7141 atgcagagag cgccacgccg tccacgcgca gcgtcacttg ctccttgaag tagcctgtcc
     7201 cgaggtcctc gagccggtcc agccgggcga tgtccggggt cgcaagcgaa tacaccgccc
     7261 cataggccag cgatcccgaa tcgtccgtca actccaggtc gcacttggcc gaaccgtcca
     7321 tgccgcgctt gtgaaaggcc aggcgatagc ctggcaacgg ggtcgtgccg accaggcgcg
     7381 cgctcgggac ccgctgcgcc agccgcaaag gatgcaggtt ggacccatag gcgaggtaat
     7441 agcgctcggt catgcccact gcccttcggc gccggcggcg tgcttcgctg gacgccggcc
     7501 gcactgcaag caacagccac ccggttgcat cccggcgtca cgccgggcgc aaccgatccg
     7561 ctacgggttg tcacactccg cgatttcgtc ttccgtcagc ccgaggtcac gcgcggcacc
     7621 ggccaggtcg tccgccgtcg cgccggctgc atgcacgtcg ctggcgatct gccccacatc
     7681 ctggttaccg aatctggacg ccgtacgggc tgccgcgttc aacagcttgc cgaagcctct
     7741 tttcttcttc tgcgccgcct gggcggcggc gaccttctcc tgcaggcagg cctggcgggc
     7801 cgcttccgcg ctggccggcg cgtgcgtcgc gccggcagac ggctggaccg gcatggccgc
     7861 acccatggac gcgggcatgc ccggcgtccc tcccccgaag ggactgcgcg ccagcgtcat
     7921 cgggttgggc accggcacgt tacagcgcat gtcccgcacg ttcgacacga cttcgatgcc
     7981 gccgccggcc gccatgttgc gcatcatctc catctgcggg cccatctgtt tcatgatcat
     8041 gtcgcgctgc ccggggggca tctgcgccag ctgcttgtcc atctcggcca tctgcagctg
     8101 cgcctcacgc atctgcgcct cctgctcggc gttcatcacg ccggcaatgc gagtcaccgt
     8161 cttgaacggc cggtacatgc ggccgcaacc aggcacctgg cggtactcct ggtcatgccg
     8221 ctcaatagtg atatcgcgtg tatcgccacc ttctcgcatc gtgccatcca tccgcaggga
     8281 caaaggcacg tagcgctcgg catccacgaa catgttgacg tcgcgtatgg tgatctcgac
     8341 accgtcctcc acctgccggt gattgacgtc agcggccagc aggtgcaggg ccggtcgccc
     8401 gtcgacggtg gtctggccga ccagccgcgt cctggcggcg atctgcgccc gctcgtgtgc
     8461 cgcatccacg gccctcgcat cgcgacgcgc ctgctcctcc ttgccgtccg ccgcgccccg
     8521 cagcatcatg ccgtacatct gggtcatgtc gttcgggttg gccgacagcc agggctggtc
     8581 aggcggcgcc gtcatcagca tgtcgcccag cccgcccggc aggccggatt tctgcatttc
     8641 ctcccgcatg ccgcgct
//
LOCUS       DAOMPG010000094         8617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3520_length_8617_cov_4.591918, whole genome shotgun sequence.
ACCESSION   DAOMPG010000094 DAOMPG010000000
VERSION     DAOMPG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_8617_cov_4.591918"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..436)
                     /locus_tag="PLS34_06655"
     CDS             complement(206..436)
                     /locus_tag="PLS34_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87183.1"
                     /translation="MSEPDTNDSNDDDIEDSFGDSEDEMEEEELDGLLEDLPAGPSAR
                     KPSDSARRRVEEYMEMKRAAKELADLDSFDFE"
     gene            complement(592..1809)
                     /gene="gspF"
                     /locus_tag="PLS34_06660"
     CDS             complement(592..1809)
                     /gene="gspF"
                     /locus_tag="PLS34_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HRP87184.1"
                     /translation="MGAFEYTAVDRRGRQQKGVLEGDTARQVRQLLRERGMLPMAITE
                     VMERERGGRRRSFSFRRGISAADLALVTRQLATLLRSGMPLEETLLAVGQQAEKPRLK
                     SILLGVRSRVMEGHTLAAGLADFPQAFPEIYRATVAAGEQSGHLDPVLERLADYTESR
                     QQLRQRVSHALIYPVILTVMAIGIVSGLLGFVVPKVVGVFENTGQALPLFTRMLIASS
                     DFLREYGVVVLVLLALGAFVFSRMLRRLPFRRRWHRFLLRVPLVGKLTRGINTARFTR
                     TLSILAGSGVPVLEALRIAGDVVVNVPMREAIHETAVRVREGAPLGRSLGASRLFPPM
                     TMHLISSGEASGELESMLERAADNQEREVDSLVGALLSILEPALIVLMGVLVFAIVMA
                     ILLPIFQLNQLVS"
     gene            complement(1838..3331)
                     /gene="gspE"
                     /locus_tag="PLS34_06665"
     CDS             complement(1838..3331)
                     /gene="gspE"
                     /locus_tag="PLS34_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRP87185.1"
                     /translation="MAPRTRGGRGRLLPFAFAKRHGILITELDDQRGQAVCRPDIKPL
                     SLAEARRFARVPIEVREVAEEEFDRLLQRAYESGSSAAMQMAEGLESDQDLSRVAQDL
                     PEPSDLLESEDDAPIIRLINAILTQAVKENASDIHIEPFENRLVVRFRIDGVLREVVQ
                     SRRAVAPLVVSRIKVMSRLDIAEKRVPQDGRISLKVAGRAVDVRVSTIPSGHGERVVM
                     RLLDKQAGKLDLSSLGMDPQTEAMMDRLIHRPHGIILVTGPTGSGKTTSLYAGLERIN
                     QASRNIMTVEDPIEYYIDGLGQTQVNTKVGMTFARGLRAILRQDPDVVMVGEIRDLET
                     AEIAVQASLTGHLVLSTLHTNTAVGAVARLRDMGVEPFLLASSLLGVMAQRLVRTLCK
                     ECRKAAPPNESESRILGVTAAGGTRIYHAVGCPACNGSGYRGRSGIYELIPVDDHLQA
                     MIHQGESEQALEKYARTLSPGIRDDGIRKVLDGTTTLEEVIRVTRDD"
     gene            complement(3375..5441)
                     /gene="gspD"
                     /locus_tag="PLS34_06670"
     CDS             complement(3375..5441)
                     /gene="gspD"
                     /locus_tag="PLS34_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HRP87186.1"
                     /translation="MKNNQGCAPQRPRRLAGIIAALAIALASPAAWSQQATITPNYKD
                     ADLRQIIEAVSAITGRNFIVDPRVRAQVTMLSAEPMSPDAFYAAFLSILEVHGYIAVP
                     SGDLIKILPDANARQVPGIDMPRGAAGSPDEVVTQVVTIRNVGAAQLVPILRPLIPQY
                     GHLAAHQASNMLIISDRAANVNRLIRIIQRIDMGADADIEVIPLQHASSSDVVRVVTA
                     LQQAAAGGETGKPLGLVADDRTNSVLISGDQTARLRLRALISHLDTPLEDGGNTQVIY
                     LRYADAEDLATRLREQVQAVTTSGGGGGQPGQASSPAARQEGVVIWADPGTNALVITA
                     PAEVMRSLRMVIDKLDIRRAQVLVEAIVAEVAYNRAAELGVTWAVDGSQDNNIIGLTK
                     LGSGGNISDLAGAALAGESAIGQALGALPQGILLGAGRLNSTSGTNWGVVLRALLSDS
                     DTNVLSTPSIVTMDNEEAEIKVGQEVPFLTGQFTNTGAAQGVVNPFQTIDRKDVGLTL
                     KVTPQINEGNAVVMTVALESSNLSRAGTGGAVDLITNQRSITQKVLIENGEVLVLGGL
                     VSDDLIQTEQRVPVLGSIPILGHLFRSQSATTEKRTLMVFLRPVILRDGVQASMETNA
                     KYLQMQGAQEGAAGRGSQFLNRNVPRPRIPAFEELTPASAGYVPPPAEPEEDDRDQPP
                     GGARGD"
     gene            complement(5459..6409)
                     /gene="gspC"
                     /locus_tag="PLS34_06675"
     CDS             complement(5459..6409)
                     /gene="gspC"
                     /locus_tag="PLS34_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HRP87187.1"
                     /translation="MAAVLSRINQWTRLPPAQLWADASRVLPPWVVLLLVVLICWQLA
                     KAVWLLFPSPAPDSLPPVQPSQRSGPAAATDGGSIDRIVAARLFGEVDAEAPVVLVEQ
                     EDAPDTQLSLELRGTVASPDERRALAIIAERGGAERVYFIGDAVPGGATLNTVHMDRV
                     LLRRAGRLEALRLPRSEDSPGPRQAAPARAAARPSPSAARAATLQQVVQQGGAALTEI
                     IRPQPVFKDGQQQGYRVYPGRQRQQFTQLGLRPGDLITQINGMSLDDPARGMEIFRSL
                     ADATSVTVTVDRNGEIEMLTLDTAVLQQQLQSTENENPSE"
     gene            6655..6972
                     /gene="clpS"
                     /locus_tag="PLS34_06680"
     CDS             6655..6972
                     /gene="clpS"
                     /locus_tag="PLS34_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRP87188.1"
                     /translation="MSDPRQKQDQDMALEEARPDVKRPPLFRVVMLNDDFTPMEFVVE
                     VLEKFFGLDRTRATRVMLEVHTRGRGVCGVFPYDIAETRVVQVTDYARQHQHPLMCTM
                     EEA"
     gene            6978..>8617
                     /locus_tag="PLS34_06685"
     CDS             6978..>8617
                     /locus_tag="PLS34_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87189.1"
                     /translation="MLSSELEFCLNEAFRRAREQRHEFMTVEHLLLALLDVPASIDIL
                     KACGVNLTTLRRELGEFIDETTPRLGDEDEQDVQPTLGFQRVLQRAVFHVQSSGRKEV
                     TASNVLVAIFGEKQSHAVYLLSLQDVSRLDVVNYISHGLTRAGEEPQEKPEAGEGNEP
                     EKDAEGDPLEKYASNLNRLAADGKIDPLIGRELEIERTIEILCRRRKNNPLYVGEAGV
                     GKTALAEGLALMIVEGRVPDVLNDCTIYALDMGTLVAGTKYRGDFEKRLKAVIAALRK
                     NPGAILFIDEIHTVIGAGAASGGVMDASNLIKPVLANGQLRCIGSTTYQEYRGIFEKD
                     HALARRFQKIDVLEPSVAETVEILKGLQSRFEEHHGIRYSEDAIRAAADLSARHINDR
                     HLPDKAIDVIDEAGASLRLQSPDPANAVVEVELIEKIVAKMARIPPKSVSASDKDLLK
                     NLERTLKLVIYGQDTAVETLASAIKMARSGLRDEHKPVGSFLFAGPTGVGKTEVTRQL
                     AMALGIELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGG"
BASE COUNT         1326 a         2869 c         3042 g         1380 t
ORIGIN      
        1 gcgcaggccg gcctatggcg ccgcgcgccc gcggtcgact ggaaccgaag gccccacgtc
       61 gaccggaacg aaaggccccg cgcccacggt cgaccgaaaa tcgggctcta agcccggttt
      121 agccggcagg gtgacaactc aaacaaaaac cgggccttgc ggcccggtct tcaagtagtt
      181 tcaacggctc cgcgcccgtg gggcatcact cgaagtcgaa actgtcgaga tcggccagct
      241 ccttggcggc tcgcttcatc tccatgtact cctccacgcg gcggcgggcg ctgtcgctgg
      301 gcttgcgcgc ggaggggccg gcggggagat cttcaaggag accgtcaagc tcctcttctt
      361 ccatctcgtc ctcgctatca ccgaaggagt cttcgatgtc gtcatcgttg ctgtcgttgg
      421 tgtccggctc gctcatgaca ttcgatctgg cacgaaaaac aacgcccccg ggggtcccgg
      481 ggcgcgacat ataccgtgcg ccgggcgcag tggcaatagc cgggcggaaa tttcacacaa
      541 ttgcagcgcc cgcgccgggg gcgtttcgcg ccgggccggc ggggaatgcg ctcaggagac
      601 cagctggttg agctggaaga tgggcagcag gatcgccatc acgatggcga acaccagcac
      661 gcccatgagc acgatcaggg ccggctcgag gatcgacagc agggcgccga ccaggctgtc
      721 cacttcgcgc tcctggttgt cggcagcgcg ttccagcatg ctctcgagct cgccgctggc
      781 ctcgccgctg gagatcaggt gcatggtcat gggcgggaac agccggctgg cgccgagcga
      841 gcggcccagc ggggcgccct cgcgcacgcg cacggccgtc tcgtggatgg cctcgcgcat
      901 gggcacgttg acgaccacgt cgccggcgat gcgcagcgcc tcgagcaccg gcacgccgct
      961 gccggccagg atgctcaggg tgcgggtgaa gcgcgcggtg ttgatgccgc gcgtcagctt
     1021 gccgaccagc ggcacccgca gcaggaagcg gtgccagcgg cgccggaacg gcaggcggcg
     1081 aagcatgcgc gagaagacga aagcgccgag ggcgagcagc accagcacga cgacgccgta
     1141 ctcgcgcagg aagtcgctgc tggcgatcag catccgcgtg aacagcggca gcgcctggcc
     1201 ggtgttttcg aacacgccga cgaccttggg tacgacgaag ccgagcaggc cgctgacgat
     1261 gccgattgcc atcacggtga ggatcacggg atagatcagc gcgtgggaga cccgctggcg
     1321 cagctgctgg cggctttccg tgtagtctgc gaggcgctcc agcaccgggt cgagatggcc
     1381 cgactgctcg cccgcggcga cggtggcgcg gtagatctcg ggaaaggcct gcgggaagtc
     1441 cgccagcccg gcggccagcg tgtggccttc catgacccgc gagcggacgc cgagcaggat
     1501 gcttttcagg cgcggctttt ccgcctgctg cccgacggcg agcagggttt cctcgagcgg
     1561 catcccggag cgcagcagcg tggcgagctg tcgcgtgacc agggcgagat ccgcggcgct
     1621 gatgccgcgg cgaaagctga agctccggcg ccggccgccg cgctcccgct ccatgacctc
     1681 ggtgatggcc atgggcagca tgccgcgctc gcgcagcagt tggcgcacct ggcgcgcggt
     1741 gtcgccctcg agcacgccct tctgctggcg cccgcgccgg tcgactgcgg tgtattcgaa
     1801 tgcgcccatg gttcaggcga agcggcgccg gtgggtctca gtcatcgcgc gtgacgcgaa
     1861 tgacttcctc gagcgtggtg gtgccgtcga ggaccttgcg gatgccgtcg tcgcggatgc
     1921 cggggctgag cgtgcgcgcg tatttttcca gcgcctgctc gctctctccc tggtggatca
     1981 tcgcctgcag gtgatcgtcc acggggatca gttcgtagat gccgctgcgg ccgcggtagc
     2041 cgctgccgtt gcaggccggg cagccgacgg cgtgataaat gcgcgtgccg ccggcggcgg
     2101 tcacgccgag tatccgcgat tcgctctcgt tgggcggcgc ggccttgcgg cattccttgc
     2161 acagcgtgcg caccaggcgc tgcgccatca cgccgagcag gctggaagcc agcaggaagg
     2221 gctcgacccc catgtcgcgc agccgcgcca cggcgccgac ggccgtgttg gtgtgcagcg
     2281 tggaaagcac caggtggccg gtgaggctgg cctggaccgc gatctcggcc gtctccaggt
     2341 cacggatctc gccgaccatg accacgtcgg ggtcctgccg caggatggcg cgcaggccgc
     2401 gggcgaaggt catgccgacc ttggtgttga cctgggtctg gccgaggccg tcgatgtagt
     2461 actcgatcgg atcctcgacc gtcatgatgt tgcggctggc ctggttgatg cgttccaggc
     2521 cggcgtacag cgacgtcgtc ttgcccgagc cggtcggccc ggtgaccagg atgataccgt
     2581 gcggacggtg gatcagccgg tccatcatcg cttcggtctg cgggtccatg ccgaggctgg
     2641 agaggtcgag cttgccggcc tgcttgtcca gcagtcgcat caccacccgc tcgccgtgtc
     2701 ccgagggaat ggtggagacg cgcacgtcga ccgcgcggcc ggcgaccttc agggagatgc
     2761 ggccgtcctg cggcacgcgt ttctcggcga tgtcgagccg cgacatgacc ttgatgcgcg
     2821 acacgaccag cggcgcgacg gcgcgccgcg actgcaccac ctcgcgcagc acgccgtcga
     2881 tgcgaaagcg caccaccagg cggttctcga agggctcgat gtggatgtcg gaggcgttct
     2941 ccttgacggc ctgcgtgaga atggcattga tcaagcggat gatcggcgca tcgtcctcgc
     3001 tttccagcag gtcggagggc tccggcaggt cctgcgcgac gcgcgacagg tcctggtcgc
     3061 tctccaggcc ctcggccatc tgcatggcgg cgctggagcc gctttcgtag gcgcgctgca
     3121 gcagccggtc gaactcttct tccgcgacct cgcgcacctc gatgggcacg cgcgcgaaac
     3181 gacgcgcttc ggcaagactc agcggcttga tgtccggcct gcagaccgcc tggccgcgct
     3241 ggtcgtcgag ctcggtgatg aggatgccgt ggcgcttggc gaaggcgaag ggcaacagtc
     3301 ggccgcggcc gccgcgggtg cgcggcgcca ggggctcggg cgtagtgccg ggcggcgcct
     3361 gcaggtcgtt cggctcagtc gccacgcgcc cctccgggcg gctggtcgcg atcgtcctcc
     3421 tccggctccg ccggcggcgg cacatagccc gccgaggccg gcgtcagctc ctcgaatgca
     3481 ggaatacgcg gccgcggcac gttgcgattg aggaactggc tgccacgccc cgcggcgcct
     3541 tcctgggcgc cctgcatctg caggtacttg gcattggtct ccatcgacgc ctggacgccg
     3601 tcgcgcagga tcaccggccg caggaacacc atcagggtgc gtttttcggt ggtcgcgctc
     3661 tgcgagcgga acaggtggcc gaggatcggg atgctgccga gcaccgggac ccgctgttcg
     3721 gtctggatga gatcgtccga caccaggccg ccgaggacca gcacctcgcc gttctcgatc
     3781 aatactttct gcgtgatgct gcgctggttg gtgatcaggt ccacggcgcc gccggtgccg
     3841 gcgcggctca ggttcgagct ctccagcgcg accgtcatga ccacggcgtt gccctcgttg
     3901 atctgcggcg tgactttcag ggtcaggccg acgtccttgc ggtcgatcgt ctggaaggga
     3961 ttcacgacgc cctgtgcggc gcccgtgttg gtgaactgcc cggtcaggaa cggcacttcc
     4021 tggccgacct tgatctccgc ttcctcgttg tccatggtga cgatcgaggg tgtggacagg
     4081 acgttggtgt cggagtcgct caacagtgcc cgcaacacca cgccccagtt ggtcccggag
     4141 gtgctgttga gccgcccggc gccgagcagt atgccctgcg gcagcgcgcc gagggcctgg
     4201 ccgatggcgg attcccccgc cagcgccgcg ccggcgagat cggagatgtt gccgccggag
     4261 ccgagcttgg tcaggccgat gatgttgttg tcctggctgc cgtccaccgc ccaggtgacg
     4321 ccgagctcgg cggcgcggtt gtaggcgacc tcggccacga tcgcttcgac cagcacctgg
     4381 gcgcggcgga tgtcgagctt gtcgatcacc atgcgcaggc tgcgcatgac ttccgccggc
     4441 gcggtaatga cgagcgcatt ggtgccggga tcggcccaga tcaccacgcc ctcctggcgg
     4501 gcggcgggtg agctcgcctg cccgggctgg ccgccaccgc ccccgctcgt cgtgacggcc
     4561 tggacctgtt cgcgcagccg tgtcgccagg tcctccgcgt cggcgtagcg caggtagatg
     4621 acctgggtgt tgccgccgtc ctcgagcggc gtgtcgaggt gcgagatcag cgcccgcagc
     4681 cgcagccgcg ccgtctggtc gccgctgatc agcacgctgt tggtgcgatc gtcggccacc
     4741 aggccgagcg gcttgccggt ttcgccaccg gcggccgcct gttgcagcgc cgtcaccacg
     4801 cgcaccacgt cgctggagga ggcgtgctgc agggggatga cttcgatgtc ggcatccgca
     4861 cccatgtcga tgcgctggat gatccggatg aggcggttca cgttggccgc gcggtcggaa
     4921 atgatcagca tgttggaggc ctggtgggcc gccaggtggc cgtactgcgg gatgagcggc
     4981 cgcaggatcg gcaccagctg cgccgcgccg acgttgcgga tggtgaccac ctgggtgacg
     5041 acctcgtccg ggctgccggc ggccccgcgc ggcatgtcga tgccgggcac ctggcgggcg
     5101 ttggcgtccg gcaggatctt gatgaggtct ccggacggca ccgcaatgta gccgtgcacc
     5161 tcgaggatcg acaggaaggc cgcatagaag gcgtccgggc tcatcggctc ggccgacagc
     5221 atggtcacct gcgccctgac gcgcggatcg acgatgaagt tccggccggt gatcgcgctg
     5281 accgcctcga tgatctggcg caggtcggca tccttgtagt tcggcgtgat ggtcgcctgc
     5341 tgcgaccagg ccgccggcga ggccagggcg atcgccagcg cggcgatgat ccccgccagg
     5401 cgccgcggcc tctgcggtgc gcatccttga ttgttcttca tggcagtccg ccgtgggctc
     5461 attctgaagg gttctcgttt tcggtgctct gcaactgctg ctgcaggacc gcggtgtcca
     5521 gcgtcagcat ctcgatttcg ccgttccgat ccacggtcac ggtaaccgag gtcgcgtccg
     5581 ccaggctgcg aaagatctcc atgccgcgcg ccgggtcgtc cagcgacatg ccgttgatct
     5641 gggtgatcag gtcgcccgga cgcaggccga gctgcgtgaa ctgctggcgc tgccggccgg
     5701 gatacacacg gtaaccctgc tgctgcccgt ccttgaacac cggctggggc cggatgatct
     5761 cggtgagcgc ggcgccgccc tgctggacga cctgctggag cgtggcggcg cgcgccgcgc
     5821 tggggctcgg acgcgctgcg gcgcgtgcag gcgcagcctg gcgcggcccc ggcgagtcct
     5881 cgctgcgcgg cagccgcagc gcctcgagcc gtccggcccg gcgcagcagc acgcggtcca
     5941 tgtggacggt gttcagcgtg gcgccgcccg gcacggcgtc gccgatgaaa tacactcgtt
     6001 ccgcgccgcc gcgctcggcg atgatggcca gcgcccgccg ctcgtccggc gaggccaccg
     6061 tgccgcgcag ctccaggctg agctgggtgt ccggggcgtc ttcctgctcc accagcacga
     6121 ccggcgcttc ggcgtcgacc tcgccgaaca gccgtgccgc cacgatgcgg tcgatgctgc
     6181 ccccgtccgt ggcggcggcc gggccgctgc gctggctggg ctgcaccggt ggcaggctgt
     6241 ccggcgcagg ggaggggaac agcagccaca cggccttcgc cagctgccag cagatcagca
     6301 ccaccagcag taataccacc cacggcggca gcacccgcga agcgtccgcc cagagctggg
     6361 caggcggcag gcgggtccac tggttgatgc ggctcaggac tgcggccacg atactccgtc
     6421 aagtcattga tccagggcgt ccggcggctt tcgtggccgg cgtatcgacc gatgatacgc
     6481 ggtgcacgga gcgactctca agcaagccgg gcggggcccg atatattccc ctatggacct
     6541 gttccgcgtt gcggagtcaa ggggttgtca cggggggttg ccgaccctat aactgaaccc
     6601 gtcggcggcc gccatgcgaa ttcgccgcaa cagtgtcagt tacagcgagc cagaatgtcc
     6661 gatccccgcc agaaacaaga ccaggacatg gccctcgagg aggcgcggcc cgacgtcaag
     6721 cgtccgccgc tgtttcgcgt ggtcatgctg aacgacgact tcacgcccat ggagttcgtc
     6781 gtcgaggtgc tggagaagtt cttcggcctg gaccgcaccc gggcgacccg tgtcatgctg
     6841 gaagtgcaca cccgcggacg cggcgtctgc ggagttttcc cgtacgatat cgccgagacg
     6901 cgcgtcgtcc aggtgaccga ctacgcccgc cagcatcagc acccgctgat gtgcacgatg
     6961 gaagaggcct gaaagctatg ctcagcagcg aacttgaatt ctgtctcaac gaggcattcc
     7021 gccgggcgcg ggaacagcgg cacgagttca tgacggtcga gcacctgctg ctggcgctgc
     7081 tcgacgtgcc ggcctcgatc gatattctca aggcctgcgg cgtcaacctg acgaccctgc
     7141 ggcgcgagct cggcgagttc atcgacgaga ccacgccccg gctgggcgac gaggacgagc
     7201 aggacgtgca gccgacgctg ggattccagc gggtgctgca gcgtgccgtc ttccacgtgc
     7261 agtccagcgg gcgcaaggaa gtgaccgcgt cgaacgtgct ggtggcgatc ttcggcgaga
     7321 aacagtcgca cgccgtctac ctgctcagcc tgcaggacgt gtccaggctc gacgtggtga
     7381 actacatctc ccacggcctg acgcgtgccg gcgaggagcc gcaggaaaag cccgaggccg
     7441 gcgaaggcaa cgagccggag aaagacgccg agggcgaccc gctggaaaaa tacgccagca
     7501 acctcaaccg gctggcggcg gatggcaaga tcgacccgct gatcggtcgc gagctggaaa
     7561 tcgagcgcac catcgagatt ctctgccggc ggcgcaagaa caacccgctg tacgtgggcg
     7621 aggcgggggt cggcaagacg gcgctggccg agggactggc gttgatgatc gtcgagggcc
     7681 gggtgccgga cgtgctgaac gactgcacca tctacgcgct cgacatgggc acgctggtgg
     7741 cgggcaccaa gtaccggggt gacttcgaaa agcgcctcaa ggcggtgatc gctgcactgc
     7801 gcaagaatcc gggtgcgatc ctgttcatcg acgagattca cacggtgatc ggcgcgggcg
     7861 cggcctcggg cggcgtgatg gacgcctcca acctgatcaa gccggtgctg gccaacggcc
     7921 agctgcgctg catcggttcg accacctacc aggaatatcg cggcatcttc gagaaggacc
     7981 acgcgctggc gcggcgcttc cagaagatcg acgtgctcga gccgagcgtg gccgagacgg
     8041 tggagatcct caagggcctg cagtcgcgct tcgaggaaca ccacggcatc cgctactcag
     8101 aagacgccat ccgggcggcg gccgacctct cggcgcgcca catcaacgac cggcacctgc
     8161 cggacaaggc gatcgacgtg atcgacgagg cgggggcgag cctgcgcctg cagtcgccgg
     8221 atcccgccaa cgccgtggtc gaggtcgagc tgatcgagaa gatcgtcgcc aagatggcgc
     8281 gcattccgcc caagagcgtg tccgcgtccg acaaggacct gctcaagaac ctcgagcgca
     8341 cgctcaagct ggtcatctac ggccaggaca cggcggtcga gacgctggcc tcggccatca
     8401 agatggcgcg ctcggggctg cgcgacgagc acaagccggt cggcagcttc ctgttcgccg
     8461 gcccgaccgg cgtcggcaag accgaggtga cccgacagct ggccatggcg ctaggcatcg
     8521 agctggtgcg cttcgacatg tcggagtaca tggagcgcca cacggtgtcg cgcctgatcg
     8581 gcgcgccgcc gggctacgtc ggtttcgacc agggcgg
//
LOCUS       DAOMPG010000095         8527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3565_length_8527_cov_4.851865, whole genome shotgun sequence.
ACCESSION   DAOMPG010000095 DAOMPG010000000
VERSION     DAOMPG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_8527_cov_4.851865"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /gene="ubiH"
                     /locus_tag="PLS34_06690"
                     /gene_synonym="visB"
     CDS             complement(<1..1036)
                     /gene="ubiH"
                     /locus_tag="PLS34_06690"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HRP87190.1"
                     /translation="MGGREAQDYDVVIAGGGMVGGSLAVALASLPLRTLVIEALPPSA
                     AAQPSYDDRATAISASSRRILEGIGCWSALAGAAAPIRAVHVSERGAFGATRLRAAEQ
                     GLPSFGHVVENRALGVALWERMNAESRLSTWTPARVVGATQDEERVRVQVERDAAAPV
                     EVSARLLVAADGARSPLRTMAGIAHEARDYGQAAVIANVTPAIDHGGTAFERFTPSGP
                     LAVLPLTKGRVTIVWTLPPADAQAVLALDDAGFLAALQAAFGYRLGGFRKAGRRASYP
                     LSLVQAQAWHAGRIALVGNAAHGIHPVAGQGFNLGLRDAAALAELLARQGPTGDPGAG
                     TMLAEYAQWRE"
     gene            complement(1042..2349)
                     /gene="pepP"
                     /locus_tag="PLS34_06695"
     CDS             complement(1042..2349)
                     /gene="pepP"
                     /locus_tag="PLS34_06695"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HRP87191.1"
                     /translation="MNTKEFARRRRQLMRMIGRGGIAILPAAPTRVRNRDVEYAYRQD
                     SDFHYLTGFPEPEAVAVLMPGREHGEYVLFCRDRDPLRETWDGRRAGPEGAVRDHGAD
                     DAFPIGDIDDILPGMLEHSERVYYTMGLSGEFDQRLLGWVNQLRAAAKSGVTTPTEFV
                     ALDHLLHDMRLFKSRGEVAAMRKAAKIAAAAHVRAMRACRPGMMEYEIEAEFLHEFRR
                     HDATTSYHPIVGSGANGCILHYVENRDRLEEGDLLLIDAGCEYDYYASDITRTFPVSG
                     RFSPEQRALYEVVLEAQLAAIDKVRPGNHWDDPHDAAVRVLTRGLVRLGLLKGRVPNL
                     IKNGDYRRFYMHRTGHWLGLDVHDVGDYKVGEEWRILEPGMTLTVEPGLYIAPGSRGV
                     AKKWWGTGIRIEDDVLVTGSGPDVLSADAPKDPDEIERLMQAA"
     gene            complement(2384..2932)
                     /locus_tag="PLS34_06700"
     CDS             complement(2384..2932)
                     /locus_tag="PLS34_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRP87192.1"
                     /translation="MTARDYGELQTALGADKGGPAGAEAQGTLCGLLCANAGDLPDTW
                     IQNTLADAEDYQFGGRDDARELLEALHGWTEQSLKAGDMSLRLMLPEETAAIEDRAAA
                     LAAWCNGFLYGLAIRGLQPVEELPEEIREILTDFAEIGRAGVAAEEVEEAGETALTEL
                     VEYVRVAAQLVYEECRPPPGYH"
     gene            3065..3295
                     /locus_tag="PLS34_06705"
     CDS             3065..3295
                     /locus_tag="PLS34_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HRP87193.1"
                     /translation="MSESTAHAVDIELRRLERRLDELVAVCRQLKEENRSLRQRQDSL
                     MLERASLMQKNEQVRTRVEAMIGRLKTMEHGS"
     gene            3292..3606
                     /locus_tag="PLS34_06710"
     CDS             3292..3606
                     /locus_tag="PLS34_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRP87194.1"
                     /translation="MSAEPARVSVRILEKEYQFACPSDEKNDLIRSAEYLNARMKQIR
                     DSGNVVGTDRIAVMAALNLANDLLKGRNRDQSLESHVGTRLKTLRERVESALEDGKQL
                     EL"
     gene            3635..3818
                     /gene="ssrS"
                     /locus_tag="PLS34_06715"
     ncRNA           3635..3818
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLS34_06715"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3852..4448
                     /locus_tag="PLS34_06720"
     CDS             3852..4448
                     /locus_tag="PLS34_06720"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRP87195.1"
                     /translation="MRNTLRREMRQRRRGLRSFQRLLADRALVRHLVNLPAYRRARDL
                     AVYWPADGEVDVRAVAAHAWSSGKHLYLPVIGRGGAMRFARWVQHGALRPNRFGIPEP
                     VQVRQCVPAGRLDLVVMPLVAFDHRGNRLGMGGGYYDRALGGKRRRQLLVGVAFACQQ
                     APSIPAQPWDVPIDLVITERGRIPMRRPRPPGSGGAEQ"
     gene            4445..4933
                     /locus_tag="PLS34_06725"
     CDS             4445..4933
                     /locus_tag="PLS34_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRP87196.1"
                     /translation="MKYWLMKSEPESFSIEDLARAPRKTTCWDGVRNYQARNMLRDEM
                     KRGDRAFFYHSSCEPPGIVGIVEIVREGYPDHTAFDPVDHHFDPKSDPAAPRWFMVDV
                     KLVEKFPRALSLRELKQHAGDELAGFQLLARGNRLSVMPVSARHWKFVNSILKANMPA
                     KS"
     gene            5088..5360
                     /locus_tag="PLS34_06730"
     CDS             5088..5360
                     /locus_tag="PLS34_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87197.1"
                     /translation="MAVSKKKAASKKKPATKKKAVAKKKPVARKKAVAKKKPAARKKA
                     VAKKKPAAKKKAAKKKPAAKKKAAAKKKPAARKKAASKKKVASKKK"
     gene            5484..6143
                     /gene="rpiA"
                     /locus_tag="PLS34_06735"
     CDS             5484..6143
                     /gene="rpiA"
                     /locus_tag="PLS34_06735"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRP87198.1"
                     /translation="MNQEAKKAAVAAAAAAEVPEGCRLGVGTGSTVNHFIEALAARNI
                     QLGCAVSSSEATSERLARHGIQVSELNSTGDLDLYVDGADEVDPAGHMIKGGGGALTR
                     EKIVAAASKRFVCIVDDSKLVRVLGAFPLPVEVIPMARSYVARQMVKLGGLPEYREGF
                     VTDNGNVILDVRHLDIVDPVDMEDRINRIPGVVTVGIFGRRKADLVLAASDAEVKRIE
                     L"
     assembly_gap    6223..6254
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6340..6413)
                     /locus_tag="PLS34_06740"
     tRNA            complement(6340..6413)
                     /locus_tag="PLS34_06740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6379..6381),aa:Gln,seq:ctg)
     gene            complement(6439..6831)
                     /gene="rpsI"
                     /locus_tag="PLS34_06745"
     CDS             complement(6439..6831)
                     /gene="rpsI"
                     /locus_tag="PLS34_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRP87199.1"
                     /translation="MSTEMNYGTGRRKTSTARVYLRPGKGAITVNSKPLDEFFGRKTA
                     RMIVRQPLELIRMDDRFDVEVTVHGGGTTGQAGAIRHGLTRALVEYDPALKGALRQAG
                     FVTRDAREVERKKVGLRKARRATQYSKR"
     gene            complement(6849..7277)
                     /gene="rplM"
                     /locus_tag="PLS34_06750"
     CDS             complement(6849..7277)
                     /gene="rplM"
                     /locus_tag="PLS34_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRP87200.1"
                     /translation="MKTFSAKPETVRRDWYVVDAEGKTLGRLATELARRLRGKHKPEF
                     TPHVDTGDYIVVINADKVRVTGNKLDDKVYYHHTGYVGHMKSETLGKRMQRAPEQVIE
                     EAVKGMLPKNSLGRQMLTKLRIFAGTEHPHQAQQPQALEI"
     gene            complement(7415..7750)
                     /locus_tag="PLS34_06755"
     CDS             complement(7415..7750)
                     /locus_tag="PLS34_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRP87201.1"
                     /translation="MGWYRRHVFFCTNRREDSRPCCHDAGAEPARAWAKARVKALGLA
                     GPGKVRVNMAGCLDRCEEGPVVVVYPEGVWYTYVDQEDLEEIIQEHLVNGRIVARLRL
                     PAEPPVEEA"
     gene            complement(7753..8481)
                     /locus_tag="PLS34_06760"
     CDS             complement(7753..8481)
                     /locus_tag="PLS34_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRP87202.1"
                     /translation="MKINGVFVAAAAFGLGLAAPVSAFAEETPAGLNGRVGFGFAEAR
                     GNTDTLSIAGDTTMEYVTGGPWLYDGKFGFVTRDESGTTTEERYEARVSANYFWSEDD
                     YVYGRLDWRKDNFGGVREEWVPSAGFGRVLLRSEQHSLHGELGAGYRWADLSDGSSEE
                     GGVLSGGLRYKWQISATAEFIQNALVQWSDDNTYLESETGLRTNIVGNLDAKISYLVK
                     HNTDVPAGRRNTDFFTNVGLEYKF"
BASE COUNT         1439 a         2830 c         2800 g         1426 t
ORIGIN      
        1 cctcgcgcca ctgtgcgtac tccgcgagca tcgtgccagc gccgggatcg cccgtcggcc
       61 cctggcgcgc cagcagttcc gccagcgcgg cggcgtcgcg cagaccgaga ttgaaaccct
      121 gacccgccac gggatgaatg ccgtgtgcgg cattgccgac cagcgcgatg cgcccggcgt
      181 gccaagcctg cgcctgcacc agcgaaagcg ggtaggaagc gcgccgcccg gccttgcgga
      241 agccgccgag gcgatagccg aacgcggcct gcaaggccgc aaggaagccg gcgtcgtcca
      301 gcgccagcac ggcctgggcg tcggcgggcg gcaaagtcca cacgatggtc acgcggccct
      361 tggtcaaggg cagcacggcc aggggcccgc tcggcgtgaa gcgttcgaag gcggtgccgc
      421 catgatcgat tgcgggcgtg acgttggcga tgacggctgc ctggccgtag tcgcgcgcct
      481 cgtgcgcgat gccggccatc gtccgcaacg gcgatcgcgc gccgtccgcg gccaccagca
      541 ggcgcgccga tacttcgacc ggcgcggcgg cgtcacgctc gacctggacg cgcacacgtt
      601 cctcgtcctg cgtagcgccg acgacccgtg ccggcgtcca ggtgctgagt cgtgactccg
      661 cgttcatccg ctcccacagc gccacgccaa gcgcgcggtt ctccacgaca tggccaaaac
      721 tcggcagccc ctgctcggca gctcgcagcc gggtggcgcc gaatgcgccg cgttccgaca
      781 cgtgcaccgc gcgaatgggc gcggccgcgc cggccagcgc ggaccagcag ccgatgccct
      841 cgaggatgcg acggctgctt gcggagatcg cggtggcccg gtcgtcgtag ctcggctggg
      901 cggccgccga cggtggcagg gcctcgatca ccagcgtgcg cagcggcagg gaagccagcg
      961 cgaccgcgag cgaaccgccg accatcccac ccccggcgat gacgacgtcg tagtcctgtg
     1021 cttcgcgccc gcccatggcc ttcaagccgc ctgcatgaga cgctcgattt cgtctggatc
     1081 cttcggcgcg tccgccgaca gcacgtcggg cccgctgccg gtgaccagca cgtcatcttc
     1141 gatgcggatg cccgtgcccc accatttctt cgccaccccg cggctgcccg gcgcgatgta
     1201 aaggccgggc tccacggtca gggtcatgcc cggttccagg atccgccact cctcgcccac
     1261 cttgtagtcg ccgacgtcat ggacgtccag tccgagccaa tggccggtac ggtgcatgta
     1321 gaagcgacgg tagtcgccgt tcttgatcag gttcggcacc ctgcccttca gcaggccgag
     1381 gcggaccagg ccgcgggtga gcacgcgcac cgccgcgtca tgcgggtcgt cccagtggtt
     1441 gccggggcgc accttgtcga tggcggcgag ctgcgcctcg agtaccactt cgtagagggc
     1501 gcgctgctcg ggactgaagc ggcccgatac cggaaaggtg cgggtgatgt cagaggcgta
     1561 gtaatcgtat tcgcagcccg cgtcgatgag cagcaggtcg ccctcctcga gccggtcgcg
     1621 gttctcgacg tagtgcagga tgcagccgtt ggccccgctg cccacgatcg ggtggtagga
     1681 ggtggtcgcg tcgtgccggc ggaactcgtg caggaactcg gcctcgatct cgtattccat
     1741 catcccgggc cggcaggcgc gcatggcccg cacgtgcgca gcggcggcga tcttcgcggc
     1801 cttgcgcatc gcggccactt caccccggct cttgaacaag cgcatgtcgt ggagcaggtg
     1861 gtcgagcgcg acgaactcgg tcggtgtcgt cacgccgctc ttggcggccg cgcgcagctg
     1921 gttgacccag ccaagcaggc gctggtcgaa ctccccgctc agtcccatcg tgtaatacac
     1981 ccgctcgctg tgctccagca tgcccggcag gatgtcgtcg atgtcgccga tcgggaaggc
     2041 gtcgtcggcg ccatggtcgc gcaccgcgcc ctccggcccg gcgcggcgtc cgtcccaggt
     2101 ctcccgcagc gggtcgcggt cccgacagaa cagcacgtat tcgccgtgct cgcggcccgg
     2161 catcagcaca gccaccgcct ccggctccgg gaagccggtc aggtagtgga aatcactgtc
     2221 ctggcggtag gcatactcga cgtcgcggtt gcgcacgcgg gtcggcgctg cgggcagaat
     2281 ggcgataccg cccctgccga tcatgcgcat cagctggcgg cgccggcgcg cgaattcctt
     2341 ggtgttcatc tgcgagtctg tcccgggtgt gattcgaccg gcatcaatga taaccgggcg
     2401 gcgggcggca ttcctcgtac accagctggg ccgctacccg gacgtattcc accagctccg
     2461 tgagcgctgt ctcgccggcc tcctcgacct cttccgccgc aacgccggcg cgaccgatct
     2521 cggcaaagtc ggtgagaatc tcgcggattt cctcgggaag ctcctcgacc ggttgcaggc
     2581 cgcgaatggc cagcccgtag agaaagccat tgcaccaggc ggccagggcc gcggcccgat
     2641 cttcgatggc ggcagtctcc tccggcaaca tcaggcgcaa cgacatgtcg cccgccttga
     2701 gcgactgctc ggtccagccg tgcagcgcct cgagcagctc gcgcgcatcg tcgcgcccgc
     2761 cgaactggta gtcctccgca tcggccagcg tgttctggat ccaggtgtcc ggcaagtcgc
     2821 ccgcgttggc gcacagcagg ccgcacaagg tgccttgcgc ctcggctccc gccgggccgc
     2881 ccttgtcggc gcccagcgcc gtctggagtt caccataatc ccgggctgtc atcgctctgt
     2941 cgtcctgggt cgcggccgct cccggctgcg gtggtggccg gatgctaaca ctccgcccgg
     3001 cccgctcaca ggcaggcgtg ggagccgatt gaccgccctc gggggcgtca ctatagttgc
     3061 ggccatgagc gagagcaccg cacacgccgt ggatatcgag ttgagacgtc tggagcggcg
     3121 gctcgacgaa ctggtggcgg tctgccgcca gctcaaggaa gagaatcgct cgctgcgcca
     3181 gcggcaggac agcctgatgc tcgagcgcgc atcgctgatg cagaagaacg agcaggtgcg
     3241 cacgcgggtc gaggcgatga tcggccggct caagaccatg gagcacggct cgtgagcgcc
     3301 gagccggccc gggtgagcgt tcgcattctc gaaaaggaat accagttcgc ctgcccgagc
     3361 gacgagaaga acgacctgat tcgttccgcc gagtacctga acgcgcggat gaaacagatt
     3421 cgtgactccg gcaacgtcgt gggcaccgac cggatcgccg tgatggccgc gctcaacctg
     3481 gccaatgacc ttctgaaggg acgcaatcgt gatcagtccc tggagtccca cgtcgggact
     3541 cgactgaaga ctctgcgcga acgcgtggag tcggctctcg aggatggcaa gcagctggaa
     3601 ctatagctgc tcgcgacaag tcttagagtc ttggcgcctc ctgcggtgtt cgatatgggc
     3661 tgggatcctc tcgaccctaa ttaaccaccc aggggccggg ccctgtcggc gccggtgcgc
     3721 aagcccgctt ctggcggaaa gccttaggtc cgacgaccgg ccccacctat tgctctggtt
     3781 cgagagcaat ggtccgtaac ggcacaggcg ggaggcgcca ccttatctac tcgccccacg
     3841 accccatcgc gatgcggaac accttgcgcc gcgagatgcg acagcggcgg cgtggactgc
     3901 gaagcttcca gcgcctgctc gccgatcgtg cgctcgtcag gcacctggtc aatctgcccg
     3961 cctaccggcg ggcgcgggat ctggccgtct actggccggc ggacggcgag gtcgacgtgc
     4021 gtgccgtggc cgcacacgcc tggtcaagcg gcaaacatct gtacctgccc gtgatcggtc
     4081 gcggcggcgc gatgcgcttc gcgcgctggg tgcaacatgg ggcgctgcgc ccgaaccgct
     4141 tcggaattcc ggagccggtg caagtccggc aatgcgtccc ggctggccga ctcgacctgg
     4201 tggtgatgcc actggtggcg ttcgaccacc gcggtaaccg gctcggcatg ggcggcggat
     4261 actatgaccg cgcgctaggc gggaagcgac gccgccagct gctggttggc gttgccttcg
     4321 cttgccagca agcgccgtcg attcccgcgc aaccctggga cgtaccgatt gaccttgtga
     4381 tcaccgaacg cggacggatt ccgatgcgcc gtccgcggcc gccaggctca ggaggagcag
     4441 aacagtgaag tattggctga tgaaatccga accggagtct ttctcgatcg aggacctcgc
     4501 gcgcgcgccg cgcaagacga cctgctggga cggcgtacgc aactaccagg cgcgcaacat
     4561 gctccgcgat gaaatgaaac gcggtgatcg cgcgttcttc taccactcca gttgcgagcc
     4621 gccgggcatc gtcggcatcg tcgaaatcgt ccgcgagggt tacccggatc acaccgcttt
     4681 cgatccggta gatcatcact tcgacccgaa gagcgacccc gctgcaccac gctggttcat
     4741 ggtcgacgtc aagctggtcg agaagtttcc acgcgcgctg agcctgcgcg aactgaagca
     4801 gcatgccggc gacgagctcg ccggcttcca gttgctcgca agaggcaatc gcctgtcggt
     4861 gatgcctgtg tcggcaaggc actggaaatt cgtcaactcg attttgaaag caaatatgcc
     4921 tgcgaaatcg tgatgacgaa atttgagctg caaacttttc tgcccgttcg cgcctcgtga
     4981 agcggaatgt ttccgatggc gcgagacgcc gaccgacttg cgatgtttgc tggaattaat
     5041 ttgttgttgt atatacagcc tcgcgaaccc ataacacgga gatgatcatg gctgtatcga
     5101 agaagaaggc tgcgagcaag aagaaaccgg cgaccaagaa gaaggcggtc gcaaagaaga
     5161 agccggttgc tcgtaagaaa gcagtggcga agaagaagcc cgccgcgaga aagaaggccg
     5221 tagcaaagaa gaagccggct gcgaagaaga aggccgccaa gaagaagccg gctgcgaaga
     5281 agaaggccgc cgcgaagaag aagccggctg ccagaaagaa ggctgcctca aagaagaagg
     5341 tggcttcgaa gaagaagtag gcgttaccgc caggcggtag cggcgatttt cgccggccgc
     5401 ggcgagtctt gagggccggg tccgcgatga gcggctcggc cctcgctcta tccggcgccc
     5461 ggaatggcag acggaggcat ggcatgaacc aggaagcaaa gaaagcggcc gtagctgcag
     5521 ccgccgcagc ggaagtcccg gagggttgcc ggctgggggt cggcaccggc tcgaccgtga
     5581 accatttcat cgaggcgttg gcagcgcgca acatccagct tggttgcgcc gtctccagtt
     5641 ccgaggcgac cagcgagcgc ctggcgcggc acggcattca agtcagcgaa ctgaactcga
     5701 ccggcgatct cgacctctat gtcgacgggg ccgacgaggt cgacccggcc gggcacatga
     5761 tcaagggcgg cggcggcgcg ctgacacgcg agaagatcgt ggccgccgca tcgaagcgct
     5821 tcgtctgcat cgtggacgac agcaagctgg tccgcgtgct gggcgctttc ccgttgccgg
     5881 tcgaggtcat cccgatggcg cgttcctacg tggcgcgcca gatggtcaag ctgggcggcc
     5941 tgcccgagta ccgcgagggc ttcgtcaccg acaacggcaa cgtcattctc gacgtgcgcc
     6001 acctcgacat cgtcgatccg gtcgacatgg aggatcgcat caaccggatt ccgggcgtcg
     6061 tcaccgtggg tatcttcggt cgcaggaaag cggacctggt actggcggca tccgacgccg
     6121 aggtgaagcg gatcgagttg tagggcgggg tctcccgcga agggggacca ggattgctcg
     6181 ccgcctgcgg cggctcgggc ctccggcccc gatgccgctc ccnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnngcctcg gctcgaaact tccaggttcg aaccctggtt ccgaatcaag
     6301 tacgcaaaag ggccccacga gggggccctt ttgcgtactt ggctggggga ccaggattcg
     6361 aacctgggtt ggcggagtca gagtccgcag tcctaccgct agacgatccc ccaaatcggg
     6421 aagcgtggtc cctgcagatc agcgcttcga gtactgggtg gcgcgccggg ccttgcgcag
     6481 gccgaccttc ttgcgctcga cctcgcgcgc gtctcgggtc acaaaacccg cctggcgcag
     6541 cgcgcccttc aacgccggat catattcgac cagggcgcgg gtcaggccat gacggatggc
     6601 gccggcctgg ccggtggtgc cgccgccgtg cacggtgacc tcgacatcga agcggtcgtc
     6661 catgcggatc agctcgagcg gctggcgaac gatcattcgc gcggtcttgc ggccgaagaa
     6721 ctcgtccagc ggcttgctgt tgacggtgat ggcgcccttg ccgggacgca gatacacgcg
     6781 cgccgtcgaa gtcttgcgcc gcccggtgcc gtaattcatt tcggtgctca tatcgattcc
     6841 tggccggttc agatctccag cgcctgcggc tgctgggcct ggtgtgggtg ttccgtgccg
     6901 gcgaagatgc gcagcttggt cagcatctgt cggcccagtg aattcttcgg cagcatgccc
     6961 ttgaccgctt cctcgatgac ctgctcggga gcgcgctgca tgcgcttgcc gagggtctcg
     7021 gacttcatgt ggccgacata cccggtgtgg tggtagtaca ccttgtcgtc cagcttgttg
     7081 ccggtgacgc gcaccttgtc ggcgttgatg acgacgatgt agtcgccggt gtcgacatgc
     7141 ggcgtgaatt ccggcttgtg cttgccacgc aagcggcgcg caagctcagt cgcaagccgc
     7201 ccgagcgtct tgccttcggc gtccacgaca taccagtcgc ggcgtacggt ttccggcttt
     7261 gcggaaaaag tcttcatgtc ccagctccag cgaggatgag cggcaggcga aaagcacgca
     7321 aatataaagg acgccctgcg gccatgcaag caggcgaccc ggggcgcatc ctgccggcgg
     7381 gccaaatcct cattaaaatc atgaactccg gccctcaggc ctcctccacc ggcggctcgg
     7441 cgggcaaccg cagccgtgcc acgatgcggc cgttgaccag gtgctcctgg atgatctcct
     7501 ccaggtcttc ctggtcgacg taggtgtacc agacgccctc cgggtagacg acgaccaccg
     7561 ggccttcctc gcagcgatcc aggcagccgg ccatgttcac ccgcaccttc cccggtcccg
     7621 ccaggcccag cgccttgacc cgggccttgg cccaggcgcg agccggctcg gcgccggcgt
     7681 catgacagca gggccggctg tcctcgcgac ggttggtgca gaaaaacacg tggcgccggt
     7741 accagcccat gctcagaact tgtattcgag cccgacattg gtgaagaagt cggtattcct
     7801 gcggccggcc ggcacatcgg tattgtgctt gaccaggtaa ctgatcttgg cgtccaggtt
     7861 gccgacaatg ttggtgcgca gcccggtctc gctttcgaga taggtgttgt cgtcagacca
     7921 ttgcaccagc gcgttctgga tgaactccgc ggtcgcggag atctgccact tgtagcgcag
     7981 tccgccgctg agcaccccgc cttcctcact gctgccgtcc gacaggtccg cccagcgata
     8041 accggcgccg agctcgccgt gcagcgaatg ctgctcagag cgcaggagga cgcggccgaa
     8101 accggcgctc ggcacccatt cctcgcgcac gccgccgaaa ttgtccttgc gccagtcgag
     8161 ccggccatag acatagtcgt cttccgacca gaagtaattg gccgacacgc gggcctcgta
     8221 acgctcctcc gtcgtcgtgc cgctctcgtc gcgcgtcacg aagccgaatt tgccatcgta
     8281 cagccacggg ccgccggtca cgtactccat cgtggtgtcg cccgcgatcg acagggtatc
     8341 ggtgttgccg cgcgcctccg cgaagccgaa gccgacgcgg ccgttcaatc cggccggtgt
     8401 ctcttccgca aatgcgctga cgggggccgc caggcccagg ccgaatgccg ccgcggcgac
     8461 gaatactcca ttaatcttca ctgcatgctc cctgtgcccg ctacgggcag agtcgataaa
     8521 cttgggc
//
LOCUS       DAOMPG010000096         8472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3590_length_8472_cov_4.403944, whole genome shotgun sequence.
ACCESSION   DAOMPG010000096 DAOMPG010000000
VERSION     DAOMPG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_8472_cov_4.403944"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLS34_06765"
     CDS             <1..740
                     /locus_tag="PLS34_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRP87203.1"
                     /translation="FTAVDSQQSLLRAVEQIGLPAILKTRRLGYDGRGQRRIRTEAEL
                     DGAFAALGGAPAILEGFVAFERELSLIGVRGRDGTTRYWPLAENVHAEGILRQTRAPW
                     GDADAQATAERHLDALLSHFDYVGVLTVEFFQADGTLLANEMAPRVHNSGHWTIEGAV
                     TSQFENHIRAILGLPLGSTAAVGHAGMVNFIGRLPPLAETLEIEGAHYHDYGKAARPG
                     RKLGHGTVVRGTAAERDSALQRMSRLA"
     gene            830..2092
                     /locus_tag="PLS34_06770"
     CDS             830..2092
                     /locus_tag="PLS34_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87204.1"
                     /translation="MYLESFGLKEHPFRLSPDPRFLFLSKQHARARAYMESTVWFSDG
                     FVVVTGEIGAGKTTLLELFLSELDDTVTVARIHQTQVTAVQFLQSLLVQFGVRPFNMR
                     KAELLDALSSHLRAVLAEGRRVVIVIDEAQNLDTTVLEEIRMLSDIGPGAGRVVSIIL
                     AGQPELNATLDSPALEQLAQRTRLRFHLKALTEQETGEYILHRLKVAGGEREIFDPES
                     FPLIYRYTGGVPRLINTLCDTAMLVAFGEERSTVGLEELREAIRELQWTDFASRTNRL
                     RAGVNFGSETIARLMVTAAGEEPHEYPLTQGRFVLGRTADNDIQLDSRFVSRHHAQLT
                     VSARSAVIEDLNSTNGIYFGGKRVKRRPLKDGDTFIIGDHELLYRVGDVASGPWVADP
                     GPTARDAELEEQDAEALEGGLEEEGKRS"
     gene            2146..3834
                     /gene="ggt"
                     /locus_tag="PLS34_06775"
     CDS             2146..3834
                     /gene="ggt"
                     /locus_tag="PLS34_06775"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP87205.1"
                     /translation="MALAGCEPATPPDAPSPVRQTAEQRTARNFMVAAAHPLAVEAGL
                     AVLRRGGSAVDAAVAVQMTLGFVEAPETGIGGGGFLLYFDAASGELSFYDGRETAPAA
                     AQPDRFTLLGLPVPRWFAMTSGRAVGVPGLVAMLALAHDRHGRLPWGELMYPAIALAE
                     SGVPMPERLRRESRRDPSLWLFRDTRRAFVAPAGEPRPLLQSPEYAATLHELATGGAA
                     SFYHGEIAAALVQRAHAGFPWASDLSPEDLAGYVPRERAPVCGPYREWTVCGAPPPSS
                     GGIALLQALGMLERFPLAQLGPRSAEAMHLLIEAQRLAFADRERFVGDPDFVDVPVTG
                     LLDADYLQRRSVLIDPARALDRALPGDPRQGLADGGGPVRTTRSSGGTSHFSIVDGDG
                     NVVALTSSNEAPFGSRMMSGGFLLNNQLTDFSFDPQHADGNHPNAVAAGKRPRSSMAP
                     VIVFDAEGRVRLALGARGGPRIIPYVLKTLVAVLDWDLGIQEAIALPNFAYADGRVEL
                     ERNTPLGMRGAEFIARGHRVRETELASGLHGIERVTERWRGGADPRLDGVARGD"
     gene            3862..4860
                     /locus_tag="PLS34_06780"
     CDS             3862..4860
                     /locus_tag="PLS34_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HRP87206.1"
                     /translation="MPPARATIRLPKKQRNHLGKPRRVGVELEMIGIELNDIAELAAE
                     HLGLSITTDGRYRRVLSGDDAGDWVVELDFDLLKRMGQEERNEEDLLDELRDSAEELL
                     KLLADLVVPLELVTPPLPMRRLDEVEELIGLLRTAGAKGTTDSLSYAFAMQFNVEIPS
                     TDADVIRRYLQAFLCLYEWLLVRADINLTRRITSYIDPFPTDYVRKVIGADYDPDLAT
                     LIDDYLADNPTRNRALDCLPLFLELDEERVRNVTEEPLIKPRPAFHYRLPDCRIDVPG
                     WGLHVAWDDWLEVEALAADRKRLTASCAAYAAFLDKPLKRWLGDWAKRVPRECLGR"
     gene            4845..5549
                     /locus_tag="PLS34_06785"
     CDS             4845..5549
                     /locus_tag="PLS34_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675510.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRP87207.1"
                     /translation="MSRPLIAITGPERRAFGPRFLVALVVRRYGGRPLQLRPSDRERG
                     FDYDGVVVTGGHDVDPVLYAAEPEVEPNHDPARDAFESAIIDEALRRCVPLLGICRGA
                     QLLNVRRGGSLFQELASRRSKTSNRWTVLPLKTLHVEPKTRLDALLGPGPRMINSLHN
                     QAIDRIGDGLRVSGRDLDGIVQAVEDPAFDYLLGVQWHPEFLVFMRRQRRLFRALVEQ
                     ARRYRASAGGYGRQET"
     gene            5704..6333
                     /locus_tag="PLS34_06790"
     CDS             5704..6333
                     /locus_tag="PLS34_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRP87208.1"
                     /translation="MIKNKTTVLLAGVACMMLSVAASADIIVNSFGMKYSGSPTGTVS
                     YPGAVSNTIYAGEIRLNTNTGPVDAFCIDVTNLLRTGSYTQSTVDLPTADRAWQSISK
                     LFDHYYEGANTASRSAAFQLALWALINGTSTSQSTSFGGAAALANSWLASLSDLDALG
                     HYQLTVLNPLAPTANQRLLTVARVPEPGTLALLGLGLLGAGAMRRRRTH"
     gene            complement(6438..7880)
                     /gene="tldD"
                     /locus_tag="PLS34_06795"
     CDS             complement(6438..7880)
                     /gene="tldD"
                     /locus_tag="PLS34_06795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HRP87209.1"
                     /translation="MKENALELTVDRLLGPAGLARGDLDRLLGRVMGRAVDAADIYIQ
                     SLRQEAWTLEDGRVKDASHMIEQGAGIRAISGEKTGFAYSDELIMPALDAASDAARAI
                     TRQGGNGAVQAWHAAAPVPLYPDLDPLGTLADEEKIALLERLDAVTRAVDPRIREVVV
                     SLVGVYELILVAASDGTLATDVRPLMRVNVSAIAEQDGRREQGFAGGGGRFGYGWLLE
                     DARAEEFAREAARQALVNLEAIAAPAGTMPVVLGPGWPGVLLHEAIGHGLEGDFNRRG
                     TSAFTGRIGQKVASPLCTIVDDGTLPGRRGSLTVDDEGTPSSCTTLIENGVLKGYLQD
                     KLNARLMGTHSTGNGRRESFAHLPMPRMTNTYMLAGPHDPEEIIASVERGLYAVNFGG
                     GQVDITSGKFVFSASEAYLVEKGRITAPVKGATLIGNGPDVLTRVSMVGNDLKLDAGI
                     GVCGKEGQSVPVGVGQPTLRIDALTVGGTA"
     gene            complement(7877..>8472)
                     /locus_tag="PLS34_06800"
     CDS             complement(7877..>8472)
                     /locus_tag="PLS34_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRP87210.1"
                     /translation="LPVLVPGEARPRAASILFDASGRAAARYDKLHLFDVSLPGADEG
                     YRESAGTCRGEGTVVADSPVGRLGLAVCYDVRFPELFRSMADAGLESISLPAAFTVPT
                     GRAHWELLVRGRAVDNLAFVLAAAQWGEHPGGRRTWGDSMIVNHWGEVLARRPAGVGI
                     VVADLDLDAQREARRRFPVLAHRLGPDALRLRAETKE"
BASE COUNT         1287 a         3022 c         2874 g         1289 t
ORIGIN      
        1 cgttcacagc ggtggactcg cagcagtcgc tgctgcgtgc cgtcgagcag atcggtctgc
       61 cggccatcct gaagacccgc cggctgggct acgacggtcg cggccagcgc cgtatccgca
      121 ccgaagccga gctcgacggc gccttcgcag cgctcggcgg cgcgccggcg atcctcgagg
      181 gtttcgtggc cttcgagcgc gagctgtcgc tgatcggagt acgcggccgg gacggcacca
      241 cccgctactg gccgctggcg gaaaacgtcc atgccgaggg catcctgcgc cagacccgtg
      301 cgccgtgggg agatgccgac gcacaggcca ccgcggagcg ccacctcgac gcgctgctca
      361 gccacttcga ctacgtcggc gtgctcacgg tcgagttctt ccaggcagac ggaacgctgc
      421 tcgccaacga gatggcgccg cgggtgcaca actcaggcca ctggaccatc gagggcgcgg
      481 tcaccagcca gttcgagaac cacatccggg cgatcctcgg cttgccgctg ggctcgaccg
      541 ccgcagtcgg gcatgccggc atggtcaatt tcatcggccg gctgccgccc ctggccgaga
      601 cgctggaaat cgagggcgcc cactaccacg actacggcaa ggccgcccgc ccgggccgca
      661 agctcgggca cggcacggtg gtgcgcggca cggcggcgga gcgcgactcc gcgttgcagc
      721 gcatgtcccg gctcgcctga atcgtgatcg gcatcgcttg cgcgcggcgg ccccgtgctt
      781 atagtggtgt cttcggggcg cgccacgacg cccgctgacc ggtcgccaga tgtacctcga
      841 atcctttgga ctcaaggaac acccgttccg cctaagcccg gacccgcgct tcctgttcct
      901 cagcaagcag cacgcccggg cgcgcgccta catggaatcc accgtgtggt tcagcgacgg
      961 cttcgtggtc gtcactggcg agatcggtgc gggcaagacc acgctgctcg agctgtttct
     1021 gtccgaactc gatgacaccg tcacggtcgc gcgcatccac cagacccagg tcactgcggt
     1081 gcagttcctg cagagcctgc tggtacagtt cggcgtccgg cccttcaaca tgcgcaaggc
     1141 ggagctgctc gacgcgctca gcagccacct tcgtgccgtg ctggccgaag gtcgccgggt
     1201 cgtgatcgtc atcgacgagg cccagaacct cgacacgacc gttctcgagg aaatccgcat
     1261 gctgtcggac atcggcccgg gcgccgggag ggtggtcagc atcatcctcg ccgggcagcc
     1321 cgagctgaat gcgacgctgg actcgccggc cctcgagcag ctggcgcagc gcacgcgcct
     1381 gcgcttccac ctcaaggccc tcaccgagca ggaaaccggc gagtacatcc tgcatcgcct
     1441 caaggtggcc ggcggcgaac gcgaaatttt tgacccggag agcttcccgc tgatctatcg
     1501 ctacaccggc ggcgtgccgc ggctgatcaa cacgctgtgc gacaccgcca tgctggtcgc
     1561 cttcggcgag gagcgcagca ccgtcggtct cgaggaactg cgcgaagcca tccgtgaatt
     1621 gcaatggacc gacttcgcca gccgcaccaa ccggctgcgc gccggcgtga atttcggcag
     1681 cgagaccatc gcccggctga tggtgaccgc ggccggcgag gagccgcacg agtatccgct
     1741 tacgcagggc cgcttcgtgc tgggccggac ggccgacaac gacatccagc tcgacagccg
     1801 cttcgtcagc cggcaccacg cgcagctgac cgtgagcgcc cgcagcgcgg tcatcgagga
     1861 cctgaacagc accaacggca tctacttcgg cggcaagcgc gtcaagcgcc ggccgctgaa
     1921 ggacggcgat accttcatca tcggcgatca tgagctgttg tatcgcgtcg gcgacgtcgc
     1981 cagcggcccc tgggtggccg atcccggccc gacggcgcgc gacgccgagc tcgaggagca
     2041 ggatgcggag gcgctcgaag gcgggctgga agaagaaggc aagaggagct gagcatcccc
     2101 ggtcgtccca ctgccgccgc ttacctggcg gcgctgttgt ttgtcgtggc cctggcgggc
     2161 tgcgagcccg ccacgccccc cgacgcaccg tccccggttc gccagaccgc cgagcagcgc
     2221 acagcgcgga atttcatggt ggcggccgcg catccgctgg cagtcgaggc cggactggcc
     2281 gtgctgcgcc gtggcggcag cgccgtcgac gcggccgtgg ccgtccagat gaccctcggc
     2341 ttcgtcgaag cacccgagac cggcatcggc ggcggcggct tcctgctcta cttcgacgca
     2401 gcgagcggtg agctcagttt ctacgacggt cgcgagaccg cgccggcagc cgcacagccc
     2461 gatcgcttca ccctgctcgg cctgcccgtg ccgcgctggt tcgccatgac cagcggccgg
     2521 gccgtcggcg tgccggggct ggtggcgatg ctggcgctgg cgcacgatcg ccacggccgc
     2581 ctgccctggg gcgagctcat gtaccccgcc atcgccctcg ccgagtctgg cgtgccgatg
     2641 cccgagcgcc tgcgccgcga gagccgtcgc gatccatcgc tgtggctgtt ccgcgacaca
     2701 cgccgcgctt tcgtggcgcc ggccggcgag ccccgtccgc tgttgcagag ccccgagtac
     2761 gccgccactc tgcacgagct cgccaccgga ggtgcggcaa gcttttacca tggcgagatc
     2821 gcggcggccc tggtacagcg ggcgcatgcc ggcttcccct gggcgagcga cctgtcgccg
     2881 gaggatctcg ccggctacgt cccgcgggaa cgcgcgccgg tgtgcgggcc ctaccgcgag
     2941 tggacggtgt gcggcgcgcc gccgccctcc tccggcggta tcgcactgct gcaggccctc
     3001 ggcatgctgg agcgctttcc gctggcgcag ctcggcccgc gctcggccga ggcgatgcac
     3061 ctgctcatcg aggcccagcg gctcgctttc gccgaccggg agcgcttcgt cggcgacccg
     3121 gatttcgtcg acgtgccagt gaccgggctg ctcgacgccg actacctgca gcgacgctcg
     3181 gtgctgatcg atcccgctcg tgcgctcgac cgcgcgctgc cgggcgaccc gcggcaggga
     3241 ctcgccgacg gcggcgggcc cgtgcggacc acccggagct cgggcggcac ctcgcatttc
     3301 agcatcgtcg atggcgacgg caacgtcgtg gcgctcacga gctccaacga ggcgccattc
     3361 ggcagccgca tgatgagcgg cgggttcctg ttgaacaacc agctgacgga cttttccttc
     3421 gacccgcaac acgccgacgg caatcatccc aatgcggtcg ccgccggcaa gcgaccgcgc
     3481 agttcgatgg cgcccgtgat cgtattcgac gccgagggcc gggtccgcct tgcactgggc
     3541 gcgcgcggcg ggccgcgtat catcccctac gtgctcaaga cgctggtggc cgtgctcgac
     3601 tgggacctcg ggatacagga agctattgcc ctgccgaact tcgcttatgc tgacggacgg
     3661 gtggaactcg agcgcaacac tccgcttggc atgcgcggcg ccgagttcat cgcgcgcggc
     3721 caccgggtcc gcgaaacgga gctggccagc gggctgcacg gcatcgagcg cgttaccgag
     3781 cgctggcggg gcggcgccga tccgcgcctc gacggcgtcg cccggggcga ctgagaccag
     3841 gaaacaggag gcaattcagc catgccgccg gcacgtgcca ccatccgact gcccaagaaa
     3901 caaagaaatc atctcggcaa gccccgccgg gtgggcgtcg agctggagat gatcggcatc
     3961 gagttgaacg acatcgccga gctcgccgcc gaacacctcg gcctcagcat cacgacggac
     4021 ggccggtacc ggcgcgtgct gagcggcgac gatgccggcg actgggtggt cgagctggac
     4081 ttcgacctcc tcaagcgcat gggccaggag gagcgcaacg aggaagacct cctggacgag
     4141 ctgcgcgact cggcggagga gctgctgaag ctgctggccg acctggtcgt gccgctcgag
     4201 ctggtcacgc cgccattgcc catgcggcgg ctcgacgaag tggaggaact catcgggctg
     4261 ctgcggaccg ccggcgccaa ggggacgacg gacagcctga gctatgcctt tgcgatgcag
     4321 ttcaacgtgg aaattccgag cacggacgcg gacgtcatcc ggcgctacct gcaggccttc
     4381 ctgtgcctgt acgagtggtt gctggtgcgc gcggacatca acctgacccg ccggatcacc
     4441 agctacatcg acccgtttcc gaccgactac gtgcgcaagg tgatcggcgc cgactatgac
     4501 ccggacctcg ccacgctgat cgacgactac ctggccgaca acccgacccg caaccgggcg
     4561 ctggattgcc tgccgctgtt cctcgagctc gatgaggaac gcgtccgcaa cgtcaccgaa
     4621 gagccgctca tcaagccgcg ccccgccttt cactatcggc tgccggactg ccggatcgac
     4681 gtgccgggct ggggcctgca cgtcgcctgg gacgactggc tggaagtgga ggcgctggcg
     4741 gccgaccgca agcgcctcac ggccagctgt gccgcttacg cggcgttcct ggacaagccg
     4801 ctgaagcgct ggctgggcga ctgggccaag agggtgccgc gcgagtgtct cggccgctga
     4861 tcgccatcac cgggcccgag cgccgcgcct tcgggccgcg tttcctggtc gcactggtgg
     4921 tgcgtcgcta cggcggccgg ccgctgcaac tgcgcccgag cgaccgggag cgcggcttcg
     4981 actacgacgg cgtggtggtc actggcggcc acgacgtcga tcccgtcctc tacgcggccg
     5041 agccggaggt cgagcccaac cacgacccgg cgcgggacgc cttcgaatcc gccatcatcg
     5101 acgaggcgct gcgccgctgc gtgccgctgc tcggcatttg ccgcggggcg cagctgttga
     5161 acgtgcgtcg tggcggcagc ctgttccagg agctggcgtc ccgccgcagc aagacctcca
     5221 accgctggac cgtactgccg ctgaagacgc tgcacgtcga gcccaagacc cggctcgacg
     5281 cactgctcgg ccccgggccg cgcatgatca acagcctgca caaccaggcc atcgaccgca
     5341 tcggcgacgg actgcgagtc tccggccgtg acctcgacgg catcgtccag gcggtcgagg
     5401 acccggcctt cgactacctg ctcggggtgc agtggcaccc ggagttcctg gtgttcatgc
     5461 gccgccagcg acggctgttc cgggccctcg tcgagcaggc ccggcggtac cgggcctccg
     5521 cgggcgggta cggccggcag gaaacgtgac gaaagtcgtg tcgggagact ttacaggccg
     5581 gcttcgcccg acgtggcgcc tgcggtctaa cctgttgttt ggattgatat tgtcttgatt
     5641 ggcacgaata ctgtatgtct tcgggcagac aaagtttatt cgcgagagga agtcgcagtg
     5701 aagatgatca agaacaagac tacggtgctc ctggccggcg tggcgtgcat gatgctgtcg
     5761 gtcgccgcca gtgccgacat tatcgtcaac agcttcggca tgaagtacag cgggtcaccg
     5821 accggcacgg tcagctatcc gggtgcggtg tccaatacga tttatgctgg tgagatccgg
     5881 ctgaacacca acaccggccc ggtcgatgct ttctgcatcg acgtcaccaa cctgctgcgt
     5941 accggctcgt acacgcagag cacggtggac ctgccaactg ctgatcgcgc gtggcagagc
     6001 atcagcaagc tgttcgacca ctattacgag ggcgccaaca ccgccagccg cagtgccgct
     6061 ttccagctcg ccctctgggc gctgatcaac ggcacgtcga ccagccagag cacctcgttc
     6121 ggcggcgcgg ctgccctcgc caattcctgg ctggcctcgc tcagcgacct ggatgcattg
     6181 ggtcattacc agctgaccgt gctcaacccg ctcgcgccga ccgccaacca gcgcctgctg
     6241 accgtggcgc gggtccccga gcccggcact ctcgcgctgc tcggcctggg cctgctgggc
     6301 gccggcgcga tgcgccgccg ccgcacgcac tgaccgactg agtcgtcatc gacaaaacgc
     6361 ccgcgaggca actcgcgggc gttttctttt gcgcgtcgga cgattcgccc ggaaccggcc
     6421 cgggcggagc ggacgtctca ggcggtgccg cccaccgtca gggcatcgat cctgagggtc
     6481 ggctggccga cgcccaccgg cacgctctgg ccttccttgc cgcacacgcc gatgccggcg
     6541 tcgagcttca ggtcgttgcc gaccatggag acgcgggtca gcacgtcggg gccgttgccg
     6601 atcagcgtgg cgcctttcac cggcgcggtg atgcggccct tctcgaccag gtaggcctcg
     6661 ctcgccgaga acacgaactt gccggaggtg atgtccacct ggccgccgcc gaagttcacc
     6721 gcgtacaggc cgcgctcgac gctggcgatg atttcctctg gatcgtgcgg gccggccagc
     6781 atgtaggtgt tggtcatgcg cggcatgggc aggtgggcga agctctcgcg ccggccgttg
     6841 ccggtggagt gcgtgcccat caggcgggca ttgagcttgt cctgcaggta acccttgagc
     6901 acgccgttct cgatcagcgt cgtgcagctg gacggcgtgc cctcgtcgtc gacggtcagc
     6961 gagccgcggc ggccgggcag cgtgccgtcg tcgacgatgg tgcacagggg cgaggcgacc
     7021 ttctggccga tccgacccgt gaaggccgag gtgccgcgcc ggttgaagtc gccctccagg
     7081 ccgtggccga tggcctcgtg cagcagcacg cccggccagc cggggcccag caccaccggc
     7141 atggtacccg ccggcgccgc gatggcctcg aggttgacca gcgcctggcg ggccgcctcg
     7201 cgggcgaatt cctcggcgcg cgcgtcctcg agcagccagc cgtagccgaa acgcccgccg
     7261 ccgccggcga agccctgttc acggcgcccg tcctgctcgg cgatggccga gacgttgacg
     7321 cgcatcagcg gccggacgtc ggtcgccagc gtgccgtcgc tggctgcaac caggatcaat
     7381 tcgtacacgc ctaccaggct gacgaccacc tcgcggatgc gcgggtcgac cgcccgcgtc
     7441 acggcgtcca gccgttccag cagggcgatc ttctcctcgt cggccagtgt gcccaacgga
     7501 tcgaggtcgg gatagagagg cacgggcgcc gcggcgtgcc acgcctgcac ggcaccattg
     7561 ccgccctggc gggtgatggc gcgcgctgcg tcggaggcgg cgtccagtgc cggcatgatg
     7621 agctcgtccg aataggcgaa gccggtcttc tcgccgctga tggcgcgaat gcctgcgccc
     7681 tgctcgatca tgtggctggc gtccttcacc cgcccgtcct cgagcgtcca ggcctcctgg
     7741 cgcagcgact ggatatagat gtccgcggcg tccacggccc ggcccatgac gcgtccgagc
     7801 aggcggtcga gatcgccgcg cgccaggccc gcggggccga gcaggcgatc gacggtgagc
     7861 tccagtgcgt tctctttcat tcctttgtct ccgcgcgcaa gcgcaatgcg tccgggccga
     7921 gccggtgtgc caggacggga aagcggcgcc gtgcctcgcg ctgcgcgtcc aggtcgaggt
     7981 ccgcgacgac gataccgacg ccggccggac gccgcgccag cacttcgccc cagtggttga
     8041 cgatcatcga gtcgccccag gtccggcgcc cgcccggatg ctcgccccac tgggccgcgg
     8101 ccagcacgaa ggccaggttg tccaccgcgc gtccgcgcac cagcagctcc cagtgcgcgc
     8161 gccccgtcgg caccgtgaac gccgccggca gcgagatcga ctcgaggccg gcatcggcca
     8221 tggaccggaa caactccggg aagcgcacat cgtaacagac cgccaggccg agccgcccca
     8281 ccggcgagtc cgccaccacc gtgccttcgc cccggcaggt gcccgcggat tcgcggtagc
     8341 cctcgtcggc gccgggcagg ctgacgtcga acaggtgcag cttgtcgtag cgcgccgccg
     8401 cgcgaccgga agcgtcgaac aggatgctgg cggcccgcgg ccgcgcttcg ccgggcacca
     8461 gcacgggcag cg
//
LOCUS       DAOMPG010000097         8382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3650_length_8382_cov_4.567311, whole genome shotgun sequence.
ACCESSION   DAOMPG010000097 DAOMPG010000000
VERSION     DAOMPG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3650_length_8382_cov_4.567311"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLS34_06805"
     CDS             <1..251
                     /locus_tag="PLS34_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRP87211.1"
                     /translation="RWRLVVANSGSRLPPGPAERLFDSLVSARPAGAAPHLGLGLFIV
                     RLVAEHHGGRATARNLPQDGGVEFVVELPRAEAGAVAT"
     gene            345..722
                     /locus_tag="PLS34_06810"
     CDS             345..722
                     /locus_tag="PLS34_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HRP87212.1"
                     /translation="MSRAFLYICGVLYLGYGLWVLLSPATSLAHLGADLSVVNTMSEL
                     RGSLGGLNVTIGLFLLWSAGHPTWFRQGLWLVFLMNVGYFGGRLFALLADGMPTGLVP
                     LVMLMEVALFVIAFVLARRANTL"
     gene            774..1526
                     /locus_tag="PLS34_06815"
     CDS             774..1526
                     /locus_tag="PLS34_06815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP87213.1"
                     /translation="MAAPGFKDHFSVAAHEYDRYRPGYPAALIDFLADAAPQRELAWD
                     CATGTGQAAVPLAGRFDCVVATDASSAQLAHAVPHPRVRYAVAPAESCGLADGVADLV
                     TVAQALHWLDLERFYAEVRRVARPGAVFAAWTYSLADADPAVDPLVAAFYEEMSPWWP
                     PERAHVEDGYRQLPFPFVPIDAPAFEIREAWPLERLLGYFSTWSGVNRYRKDTGRDPV
                     AALGRRLAAVWGDPAQPRPVRWPLHLRVGRVQ"
     gene            1523..2221
                     /locus_tag="PLS34_06820"
     CDS             1523..2221
                     /locus_tag="PLS34_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87214.1"
                     /translation="MSGTPLLTPDAALPEVARAPAPWRLRGDGWILLLRLPDEVRSDP
                     RSLPPELRDRPVSGPSMLMFVDYAASPAGPYRELLYIPGRFRTTDGTRAWSVTRIFVS
                     TWDSVVNGRLNWGIPKDLAEFTRLPSRRGERIEVRIGDRQVAAIDLEAHGPALPVHAA
                     LLPGRLRRLVQYHGTRRFEFTPGARGRASLARVGSIAADPELVGEFSGARVALALRIP
                     EFELQFPVAASRSR"
     gene            complement(2235..3548)
                     /locus_tag="PLS34_06825"
     CDS             complement(2235..3548)
                     /locus_tag="PLS34_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRP87215.1"
                     /translation="MHRGTRLLLMLLGLGCAVPAVAIEQATIAAGAAALEQKLVAWRR
                     DIHANPELSNREFRTAALVAEHLRGLGLEVRTGIAHTGVAAYLQGGRPGPMVALRADM
                     DALPVTERTGLPFASQVTAEYNGSRTGVMHACGHDAHTAILMAVAELLAGMRDELPGS
                     VLFIFQPAEEGAPEGEDGGAELMLKEGLFEWQRPDAVFGLHVLSNFNAGHIGYRPGPA
                     MAAADRFEITVKGAQTHGSMPWLGVDPIVPAAQIVLGLQTITSRQVNISDVPAVISVG
                     QIQGGIRNNIIPDDVYLQGTIRTFDEAIREDIHERVRQTADSLAAASGATAEVHVHRG
                     YPVTVNDPGLVSATLPSLRRAAGEERVFQVPRLMPAEDFSYFANEVPGFYFYLGATPE
                     GVDPAAAPTNHSPHFDVDESTLRVGLEALATVAVDFLQRGAGEAR"
     gene            complement(3599..5011)
                     /locus_tag="PLS34_06830"
     CDS             complement(3599..5011)
                     /locus_tag="PLS34_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP87216.1"
                     /translation="MSQEKPLRYLEIPRKDPRKVPPKERVRDYREIYGQYDAEGAHKQ
                     AGRCLDCGNPYCEWKCPVHNYIPNWLRLVEEGNLFEAAELCHRTNSLPEICGRVCPQD
                     RLCEGACTLNDGFGAVTIGSIEKYITDEAVRQGWRPDLSGVRTSGREVAIIGAGPAGL
                     AAADVLARHGVRPVVYDRYPRIGGLLTFGIPPFKLEKEIVETRRMLLEGMGVEFVLNC
                     EIGRDLTLDQLLADYDAVFAGTGAYRYVKGGLPGEDLPGVYEALPYLVANARRELGLM
                     ADGEEFLSMAGKRVVVLGGGDTGMDCNRTAIRQGAVGVTCAYRRDEKNMPGSRREVRN
                     SREEGVEFLFNRQPIEIVGSSAAEGVKVVRTRLGEKGPGGRRRPEPVPGTEEILPADA
                     VIIAFGFRPDPPAWLTGAGVETWPDGRLRIDAVGRYPYQTTHPKIFAGGDNVRGSDLV
                     VTAVYEGREAAKGIIAYLGV"
     gene            complement(5019..>8382)
                     /gene="gltB"
                     /locus_tag="PLS34_06835"
     CDS             complement(5019..>8382)
                     /gene="gltB"
                     /locus_tag="PLS34_06835"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRP87217.1"
                     /translation="VVTDDDHLTIASEVGVWDYRPEQVVAKGRLGPGEMIIADTATGK
                     LMEWREIDEVLASRAPFKDWLRRGVRYLETSLVDPQLLAEPMQREELVRYEKMFGVTR
                     EEREEVLRPLCEAEAEAVGSMGDDTPLPFMSRRVRSLYDYFRQQFAQVTNPPIDSLRE
                     RVVMSLETQIGREHNLFEPRPEYARRIVINSPVLSQRKFRQLLADQEQDNGYELLDLN
                     RPEAESLQDGLDRLCADAEAAVRAGKLIIVLSDRQLRPGAVPIHALLATGAVHHHLVR
                     AGLRCDCNLLVETGTVRDPHQFACLVGYGATAIYPYLAYECLNDLARSGRLKGGRESG
                     RQLGRSYRRGIRKGLFKIMSKMGISTIASYRGAALFEIVGLHDEVVARCFPGSVSRIQ
                     GADFTDLKSDAMALAQRAWNTRSEVEQGGLLRYVHGGEYHAYNPDVIATLQAAVQSGD
                     YGIYQEYARLVNERPAMVIRDLLEVKPLGPPIPLEEVEPEEALYKRFDSAGMSLGALS
                     PEAHEALAIAMNRLGGRSNSGEGGEDKARYGTEKLSKIKQVASGRFGVTAEYLVNAEV
                     LQIKIAQGAKPGEGGQLPGHKVNEMIAKLRYARPGVGLISPPPHHDIYSIEDLAQLIF
                     DLKQVNPAALVSVKLVAQAGVGTVAAGVTKAYADLITISGYDGGTGASPLTSTKYAGS
                     PWELGLAETHQVLVQNGLRHRVRLQTDGGLKTGADVIKAAVLGAESFGFGTAPMVALG
                     CKYLRICHLNNCATGVATQHPVLRERHFIGLPEMVMNYFRFVARETRELLAALGARSI
                     EELIGRADLAAPLPGETPRQRRLDLKPLLNAGGLSAGEARFCTEARNPPFDEGLLNER
                     MVADCSQAIEERRGGTFFYEVKNFHRSIGARLSGEIARRYGNLGLEADPVVIRMKGSA
                     GQSFGVWNVGGLHLYLEGDANDYVGKGMAGGRLVIYPPTGSRFEARSAPIIGNTCLYG
                     ATGGQLYAAGTAGERFAVRNSGAVAVVEGCGDHGCEYMTGGVVVVLGRTGVNFGAGLT
                     GGFAYVLDIDRDFVDRYNHELIDIHRITPEAMEAHLHHLRGLLEAHARETGSAWGREI
                     LDDFRSYLPKFWLVKPKAADLESLIDSLRRAA"
BASE COUNT         1160 a         2962 c         2922 g         1338 t
ORIGIN      
        1 atcgctggcg actggtggtg gccaactccg gcagccgcct gccgcccgga ccggcggagc
       61 gcctgttcga ttcgctggtg tcggcgcggc cggcgggcgc cgcgccgcat ctcgggctgg
      121 ggctgttcat cgtgcggctg gtcgccgagc atcacggcgg ccgtgccacg gcccgcaacc
      181 tgccgcagga cggaggcgtg gaattcgtcg tggagctgcc gcgcgccgaa gccggcgccg
      241 tcgccacctg accggccggc cggtatcggg gcgcccacgg atgcgtccgg accgcgacgc
      301 ggctatagtc ccggctggcc tcttgcaatc gcccggagca atccatgagt cgcgcgttcc
      361 tgtacatctg cggtgtgctg tacctgggct acggactgtg ggttctgctg tcgccggcca
      421 ccagcctggc gcacctcggc gcggacctca gcgtcgtcaa caccatgtcc gagctgcgcg
      481 gcagtcttgg cgggctcaac gtcaccatcg gcctgttcct gctgtggtcg gccggccatc
      541 cgacctggtt ccgccagggc ctgtggctgg tgttcctgat gaacgtcggc tatttcggcg
      601 gccgcctgtt cgcgctgctg gccgacggca tgccgacggg gctcgtgccg ctggtgatgc
      661 tgatggaagt cgcgctgttc gtcatcgcct tcgtgctggc acgacgcgcg aatacgctct
      721 gagccaaacg cgtgacggcg ctgatgcgca acccggcgga gcggccggcc tgaatggcgg
      781 cgcctggctt caaggaccat ttttcggtcg cggcccacga gtacgaccgc taccggccgg
      841 gctatcccgc ggcgctgatc gactttctgg ccgatgcggc gccacagcgg gaactcgcct
      901 gggactgcgc gaccggcacc ggccaggcgg ccgtgccgct ggccggccgg ttcgactgcg
      961 tcgtcgccac cgatgccagt agcgcccagc tggcgcacgc cgtgccacat ccgcgcgttc
     1021 gttacgcggt ggcgccggct gaaagctgtg gcctggccga cggcgtcgcg gacctggtga
     1081 ccgtggcgca ggcgctgcac tggctcgacc tcgagcgctt ctatgccgaa gtgcggcgcg
     1141 tagcgcgccc cggcgcggtg ttcgctgcct ggacctacag cctggcggac gccgacccgg
     1201 cggtcgaccc gctggtcgcc gccttctacg aggaaatgag tccctggtgg ccgcccgagc
     1261 gcgcacacgt cgaggatggc tatcgccagc tgccgtttcc attcgtgccc atcgacgccc
     1321 cggccttcga gattcgcgag gcctggccgc tggagcggct gctggggtac ttcagcacct
     1381 ggtcgggggt gaaccgctac cgcaaggaca ccggccgcga cccggtggcc gcgctcggcc
     1441 ggcggctggc ggcggtctgg ggcgatccgg cgcaaccgcg ccccgtgcgc tggcccctgc
     1501 acctgcgcgt cggccgcgtg cagtgagcgg cacgccgctg ctgaccccgg acgcagcgct
     1561 gcccgaggtt gcgcgcgcgc cggcgccctg gcggctgcgg ggcgatgggt ggatcctgct
     1621 gctgcgactg ccggacgagg tgcgcagcga tccacggtca ttgccgccgg agttgcgcga
     1681 tcggcccgtc agcgggccct cgatgctgat gttcgtcgac tatgcggctt cgcccgccgg
     1741 accctaccgc gagctgctgt acatcccggg gcggttccgg accaccgacg gcacgcgcgc
     1801 ctggtcggtc acgcggatct tcgtcagcac ctgggacagc gtggtcaacg gccggttgaa
     1861 ctggggcatt cccaaggatc ttgccgagtt cacgcgcctc ccgtcacggc gaggggagcg
     1921 catcgaggtc cggatcggcg accgccaggt cgcggccatc gacctcgagg cccacggtcc
     1981 ggcgctgccc gtgcatgcgg ccctgctgcc cggccggctg cgccgcctgg tccagtacca
     2041 tggcacacgg cgcttcgagt tcacgcccgg tgcgcgcggc cgcgcgtcgc tggcgcgcgt
     2101 cggcagcatc gcggccgacc ccgagctggt gggcgagttc tccggcgcgc gggttgccct
     2161 ggcactgcgc atcccggaat tcgagctgca gttcccggtc gcggccagcc ggtcgcgctg
     2221 agcgtcggcg tcggtcagcg cgcttcgccc gccccgcgtt gcaggaaatc gacggccacg
     2281 gtggccaggg cctccaggcc gacgcgcagc gtgctctcgt cgacgtcgaa gtgcggcgaa
     2341 tgattggtcg gcgctgcggc gggatcgacg ccctccggcg tcgcacccag gtagaaatag
     2401 aaaccaggca cttcattggc gaagtaactg aaatcctccg ctggcatcag ccgcggcacc
     2461 tggaacacgc gctcctcgcc ggcggcgcga cgcaggctcg gcagggtcgc agagaccagc
     2521 ccggggtcgt tcacggtgac cgggtagccg cgatgcacgt gcacctcggc cgtggcgccc
     2581 gaggccgccg cgaggctgtc ggcggtctgc cggacgcgct cgtgaatgtc ttcgcggatg
     2641 gcctcgtcga aagtccggat ggtgccctgc aggtagacgt cgtcggggat gatgttgttg
     2701 cggatgccgc cctggatctg gccgaccgag atcacggcgg gcacgtccga gatgttcacc
     2761 tgccggctgg tgatggtctg caggcccagc acgatctgcg ccgccggcac gatggggtcg
     2821 acgcccagcc acggcatcga gccgtgcgtc tgtgcgccct tgaccgtgat ctcgaaccga
     2881 tccgcggccg ccatcgccgg gcccgggcgg tagccgatgt gccccgcatt gaagttggac
     2941 agcacgtgca ggccgaacac agcgtccggg cgctgccatt cgaacaggcc ctctttcagc
     3001 atcagctcgg ccccgccgtc ctcgccctcc ggtgcacctt cctcggccgg ctggaagatg
     3061 aacaacacgg acccgggcaa ttcgtcgcgc atccccgcca gcagctccgc cacggccatc
     3121 aggatcgccg tgtgcgcgtc atgcccgcat gcgtgcatca cgcccgtgcg gctgccgttg
     3181 tactcggcgg tgacttgtga ggcgaagggc agcccggtcc gctcggtgac cggcagggcg
     3241 tccatgtcgg cgcgcagcgc gaccatcggg ccgggccttc ctccctgcag gtaggcggcc
     3301 acgccggtgt gggcgatgcc ggtgcgcacc tccaggccca gcccgcgcag gtgctcagcc
     3361 accagcgccg cggtgcggaa ctcgcggttg gacagctcgg ggttggcgtg gatgtcgcgg
     3421 cgccacgcga ccagtttctg ctccagcgcg gccgcgccgg cggcgatcgt cgcctgctcg
     3481 atcgcgaccg ccgggacggc gcaaccaagc cccagcaaca tcagcagcaa cctggttcca
     3541 cggtgcatcg gtttctccgg cggattcggc gatctgcgga gcttacccca gccggtcgtc
     3601 agacaccgag gtaggcgatg atccccttcg cggcctcgcg gccctcgtac accgcggtga
     3661 ccaccaggtc ggagccgcgc acgttgtccc cgccggcgaa aatcttcgga tgcgtcgtct
     3721 ggtagggata acggccgacc gcatcgatcc gcaggcgccc gtcgggccag gtctcgacgc
     3781 cggcgccggt cagccaggcc ggcgggtcgg gacggaagcc gaaggcgatg atcacggcgt
     3841 ccgccggcag gatttcctcg gtgccgggaa ccggctcggg gcggcggcgg ccaccggggc
     3901 ccttctcgcc gagccgcgtg cgcaccacct tcacgccctc ggcggcgctg ctgccgacga
     3961 tctcgatggg ctggcggttg aacaggaact cgacgccctc ttcgcggctg ttgcgcactt
     4021 cgcggcgtga tccgggcatg ttcttctcgt cgcggcgata ggcgcaggta acgccgacgg
     4081 caccctgccg gatcgcggtg cgattgcagt ccatgccggt gtcgccaccg ccgagcacca
     4141 ccacgcgctt gccggccatc gacaggaact cctcgccgtc cgccatcagg cccagctcgc
     4201 gccgcgcatt ggcgaccagg tagggcagcg cctcgtaaac acccggcagg tcctcgcccg
     4261 gcaacccgcc cttcacgtag cggtaggccc cggtgccggc gaacacggcg tcgtagtcgg
     4321 ccagcaactg gtcgagcgtg aggtcgcgac cgatttcgca gttcagcacg aactccacgc
     4381 ccatgccttc cagcagcatg cggcgcgtct cgacgatctc cttctccagc ttgaacggcg
     4441 ggatgccgaa ggtcagcagg ccgccgatgc gcgggtagcg gtcgtacacc accggccgca
     4501 cgccgtggcg cgccagcacg tcggccgcgg cgagcccggc gggaccggcg ccgatgatcg
     4561 ccacctcgcg cccggaggtc ctgacaccgg acaggtcagg gcgccagccc tggcgcaccg
     4621 cctcgtcggt gatgtacttc tcgatgctgc cgatggtgac ggcgccgaag ccgtcattga
     4681 gcgtgcaggc gccctcgcag aggcggtcct gggggcacac ccggccgcag atctccggca
     4741 gcgagttggt gcgatgacaa agctcggccg cctcgaacag gttgccctcc tcgaccagcc
     4801 tgagccagtt cggaatgtag ttgtgcaccg ggcacttcca ctcgcagtag gggttgccgc
     4861 agtcgaggca acggcccgcc tgcttgtgtg cgccctcggc gtcgtactgg ccgtaaatct
     4921 cccggtagtc gcgcacgcgc tccttcggcg gcaccttgcg cgggtccttg cgcggaatct
     4981 cgaggtaacg aagcggcttt tcctggctca tggcttgctc acgccgcgcg cctgagcgaa
     5041 tcgatgaggc tctcgaggtc ggcggccttc ggcttcacca gccagaactt cggcaggtag
     5101 gagcgaaagt cgtcgagaat ctcgcggccc caggcgctgc cggtctcgcg cgcatgcgcc
     5161 tccagcaggc cgcgcaggtg gtgcaggtgc gcctccatgg cctccggcgt gatgcgatgg
     5221 atgtcgatga gttcgtggtt gtagcggtcg acgaaatcgc ggtcgatgtc gagcacgtag
     5281 gcgaagccgc cggtcaggcc ggcgccgaaa ttcacgccgg tgcgacccag caccaccacc
     5341 acgccgccgg tcatgtactc gcaaccgtga tcgccgcaac cctcgaccac cgcgaccgcg
     5401 cccgagttgc gcactgcgaa gcgctcgccg gcagtcccgg cggcgtacag ctgcccgccg
     5461 gtggcgccgt acaggcaggt gttgccgatg atcggcgcgc tgcgcgcctc gaagcggctg
     5521 ccggtcggcg ggtagatcac caggcggccg cccgccatgc ccttgccgac gtaatcgttg
     5581 gcgtcgccct cgaggtagag atgcaggccg ccgacgttcc agacaccgaa gctctgcccg
     5641 gcggagccct tcatgcggat gaccaccggg tccgcctcga ggccgagatt gccgtaccgg
     5701 cgcgcgatct cgcccgacag ccgcgccccg atggagcggt ggaaattctt cacttcgtag
     5761 aagaaggtgc cgccgcggcg ctcctcgatg gcctggctgc agtccgcgac catgcgctcg
     5821 ttgagcagtc cctcgtcgaa cggcgggttg cgtgcctcgg tgcagaagcg tgcctcgccg
     5881 gccgacagcc cgccggcgtt gagcaggggc ttcaggtcga ggcgtcgctg gcgcggcgtc
     5941 tcgcccggca gcggtgcggc aaggtcggcg cgcccgatca actcctcgat gctgcgcgca
     6001 ccgagcgcgg cgagcagttc gcgggtctcg cgcgccacga agcggaagta gttcatcacc
     6061 atctcgggca ggccgatgaa gtgccgctcg cgcagcacgg ggtgctgcgt ggccacgccg
     6121 gtggcacagt tgttgaggtg gcagatgcgc aggtacttgc agcccagcgc caccatcggc
     6181 gccgtgccga agccgaagct ctcggcgccc agcaccgccg ccttgatgac gtcggcgccg
     6241 gtcttgaggc cgccgtcggt ctgcaggcgc acgcggtgcc gcaagccgtt ctgcaccagc
     6301 acctggtggg tctcggccag gcccagctcc cacggcgagc cggcgtactt ggtcgaggtc
     6361 agcgggctcg cgcccgtgcc gccgtcgtag cccgagatgg tgatcaggtc agcgtaggcc
     6421 ttggtgacgc cggccgccac cgtgccgacg ccggcctgcg ctaccagctt gaccgatacc
     6481 agcgccgccg gattgacctg cttcaggtcg aagatcagct gcgcgagatc ttcgatcgag
     6541 tagatgtcgt gatgcggcgg cggcgagatc aggccgacac cgggccgcgc atagcgcagc
     6601 ttcgcgatca tctcgttgac cttgtggccg ggcagctggc cgccttcgcc gggcttcgcg
     6661 ccctgcgcga tcttgatctg caggacctcg gcgttcacca ggtactcggc cgtgacgccg
     6721 aagcgcccgg aggccacctg cttgatcttg gagagcttct ccgtgccgta gcgggccttg
     6781 tcctcgccgc cctcacctga attcgaacgc ccgccgaggc ggttcatggc gatggccagc
     6841 gcttcgtggg cctcgggcga gagcgcaccg aggctcatgc cggcggaatc gaagcgcttg
     6901 tagagcgcct cctccggctc gacctcctcc agcgggatcg gcgggcccag cggcttcacc
     6961 tccagcaggt cgcgaatcac catcgcgggt cgctcgttca ccagccgcgc atattcctgg
     7021 tagatgccgt agtcgccgct ctgcaccgcg gcctgcaggg tggcgatgac gtcggggttg
     7081 taggcgtggt actcgccgcc gtgcacgtag cgcaacaggc cgccctgctc gacctcgctg
     7141 cgcgtgttcc aggcgcgctg cgcgagggcc atggcgtcgg acttcaggtc ggtgaagtcg
     7201 gcgccctgga tgcgcgacac gctgcccggg aagcagcgcg ccacgacctc gtcgtgcagc
     7261 ccgacgattt cgaacagcgc ggcgccccgg tagctggcaa tggtcgagat gcccattttc
     7321 gacatgatct tgaacaggcc cttgcggata ccgcgacgat agctgcggcc cagctgccgg
     7381 ccgctctccc gcccgccctt taggcgcccg ctgcgcgcca ggtcgttgag gcactcgtag
     7441 gccaggtagg ggtagatggc cgtagcgccg taaccgacca ggcaggcgaa ctggtgcggg
     7501 tcgcgcaccg tgccggtctc gaccagcagg ttgcagtcgc agcgcagccc ggcgcgcacc
     7561 aggtggtggt gcacggcgcc ggtggcgagc aaggcatgga tgggcaccgc gcccggacgg
     7621 agctggcgat cgctgagaac gatgatcagc ttgccggcgc gcaccgcggc ctcggcgtcg
     7681 gcgcacagcc ggtccaggcc gtcctgcagg gactcagcct cggggcggtt caggtcgagc
     7741 agttcgtagc cgttgtcctg ctcctggtcc gccagcagct ggcggaactt gcgttgcgac
     7801 agtaccggcg agttgatgac gatacgccgg gcgtactccg gccgcggctc gaacaggttg
     7861 tgctcgcggc cgatctgggt ctccagcgac atcaccacgc gctcgcgcag cgaatcgatc
     7921 ggcggattgg tgacctgcgc gaactgctgg cgaaaataat cgtacagcga gcggacgcgc
     7981 cgcgacatga agggcagcgg cgtgtcgtca cccatcgagc cgacggcctc ggcctcagcc
     8041 tcgcacagcg gccgcagcac ctcctcgcgt tcctcgcggg tgacgccgaa catcttctcg
     8101 tagcggacca gttcctcgcg ctgcatgggc tcggcgagca gctgcgggtc gaccagcgag
     8161 gtctcgaggt agcgcacgcc gcggcgcagc cagtccttga acggtgcccg gctggcgagc
     8221 acctcgtcga tctcgcgcca ttccatcagc ttgccggtgg cggtgtcggc gatgatcatc
     8281 tcgccggggc cgagccgccc cttggcgacc acctgctccg ggcggtaatc ccacacgccg
     8341 acctcggagg cgatggtcag gtggtcgtcg tcggtcacca cc
//
LOCUS       DAOMPG010000098         8327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3681_length_8327_cov_5.082809, whole genome shotgun sequence.
ACCESSION   DAOMPG010000098 DAOMPG010000000
VERSION     DAOMPG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_8327_cov_5.082809"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLS34_06840"
     CDS             complement(<1..264)
                     /locus_tag="PLS34_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRP87218.1"
                     /translation="MSPLSQTENKPLFERGPSLGTRMVLLAVLSVALMVLDHRQGHLE
                     TVRRALSVAVYPIRVLVDMPSTSFGWAREKLSERNRLVLENRDL"
     gene            complement(326..1369)
                     /locus_tag="PLS34_06845"
     CDS             complement(326..1369)
                     /locus_tag="PLS34_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRP87219.1"
                     /translation="MFKNIRGYFSNDLSIDLGTANTLIYVRGRGIVLNEPSVVAIREE
                     SSRGGRTVQAVGAEAKSMLGRTPGNITAIRPLKDGVIADFTVTEKMLQHFIKKAHGSR
                     YFRPSPRVLVCVPYGSTQVERRAIRESAEGAGARKVYLVEEPMAAAIGAGMPVQEARG
                     SMVLDIGGGTSEVAVISLNGIVYAASVRIGGDRFDEAIMSYVRRNYGILIGEATAERI
                     KHEIGAAYPGQEVREISVRGRNLSEGVPRSFTLNSNEILEALQEPLQGIVGAVKAALE
                     QTPPELGADVADRGIVLTGGGALLRDLDKLLMEETGLPVIIADEPLTCVALGGGKVLE
                     LLDQHGPGLFGIE"
     gene            1540..1827
                     /gene="gatC"
                     /locus_tag="PLS34_06850"
     CDS             1540..1827
                     /gene="gatC"
                     /locus_tag="PLS34_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRP87220.1"
                     /translation="MKLSRHEVESIAHLARLEISADEIPVYVDNLSRIIDFVQQLERA
                     DTEGVTPMAHPLNMTQPLRPDEVTESDQHALYQQNAGAVESGLYLVPRVIE"
     gene            1845..3302
                     /gene="gatA"
                     /locus_tag="PLS34_06855"
     CDS             1845..3302
                     /gene="gatA"
                     /locus_tag="PLS34_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRP87221.1"
                     /translation="MHGMTLAGIARALRAREFSSEELVSHLLERIGRLDGQLNAFVTV
                     TGEAALQAARDADRLLAEGRAAPLAGVPIAHKDIFCTAGVKTSCGSRMLDNFVSPYDA
                     TVVERLRAAGTIMLGKTNMDEFAMGSSNETSFYGPVRNPWDLERVPGGSSGGSAAAVA
                     ARLVPAATGTDTGGSIRQPAALAGITGIKPTYGRVSRYGMIAFASSLDQAGVLTQTAE
                     DAALLLGAMCGHDPRDSTSVDAPVPDYAAGLGASLEGLRIGLVEEFLDDGLDAANAAA
                     VEAALKVLERLGAEVRRVSLPNIGLSVPTYYVVAPAECSSNLSRFDGVRFGYRAAAPR
                     DLEDLYKRSRGEGFGAEVKRRIMTGTYVLSAGYYDAYYLKAQRSRQLIAADFRRAFEQ
                     VDVIVGPTSPTPAFELGAKVDDPVTMYLNDIYTIGVNLAGLPGLSVPCGFIDGLPVGL
                     QIIGRAFDEGRILNVAHGYQQETDWHTRAPGGAFA"
     gene            3312..4745
                     /gene="gatB"
                     /locus_tag="PLS34_06860"
     CDS             3312..4745
                     /gene="gatB"
                     /locus_tag="PLS34_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRP87222.1"
                     /translation="MEWETVIGLEIHAQLATRSKIFSGAATAYGAAPNTQACLVDLGY
                     PGVLPVLNEGVVRMAVAFGLATESTIAARSVFARKNYFYPDLPKGYQISQYELPIVHD
                     GRIELQLDDGVHKTVGITRAHLEEDAGKSLHEDFQGQSGIDLNRAGTPLLEIVSEPDM
                     RSTKEAVAYMKAIHQLVRYLGICDGNMQEGSFRCDANVSVRPKGRREFGTRTEIKNLN
                     SFRYVERAILFEVERQIDILESGGSVVQETRLYDPDRDETRPMRSKEEANDYRYFPDP
                     DLLPLEVGPELVEAVRAALPELPWRKRDRFMDEYGLSEYDAGVLCASRAGADYFEAVA
                     AGVADKKLAANWVMGELAGALNKAGLEVTASPVPAGALLGLLKRIEDSTISGKIAKEV
                     FEAMWAGEGDADAVIEARGLRQITDSDSVEAVIREVMAANPQQLEQYRSGKDKLFGFF
                     VGQVMKATGGKANPALVNELLTKLLAG"
     gene            4745..5632
                     /gene="hslO"
                     /locus_tag="PLS34_06865"
     CDS             4745..5632
                     /gene="hslO"
                     /locus_tag="PLS34_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRP87223.1"
                     /translation="MSGGGDALRRFMFEDFPVRGEIVRMDTSWRAVLERQDYPPAVRD
                     LLGEAMAATVLLASTLKFDGMLTLQIQGEGDLHLLVAQCGSDLAVRGLAKWKSDDPRG
                     TLAELTGGGRLAITIERRKDRERYQGIVLADTDTLAGCLETYFAQSEQLPTRIWLAAD
                     DERAAGMLLQQMPSEAEETDRDGWNRAGLLAETLSSDELLKLDARDVLRRLFHEENLR
                     LADQRGVAFHCSCTRQRVESAMRLLGRDELAELLRADGRIEVRCEFCNKAYGLDAVDV
                     EQILADAGPLQPPGSERVH"
     gene            complement(5633..6430)
                     /locus_tag="PLS34_06870"
     CDS             complement(5633..6430)
                     /locus_tag="PLS34_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRP87224.1"
                     /translation="MRLLNTLGRRIAAYLSRPRENGGRVATGSRENLAATLQRGDVLL
                     VEGTSLFASAVKYLTQSTWSHAALCVGDALGRRSQDGESLLLVEADINTGVRALPLSE
                     YWDLHTRICRPVGLSSRELDELVAFVVARIGHQYDLKNIWDLARYLMPTPPVPRRWRR
                     KLLALGSGDPTRAICSSLIAQAFGSIGYPILPELYLQDLPTDSAQYREWLHIRHHSLY
                     APRDFDVSPYFEVVKPKLARGFDHHELDWADTVRLQALDLDGPHVPA"
     gene            complement(6514..6987)
                     /locus_tag="PLS34_06875"
     CDS             complement(6514..6987)
                     /locus_tag="PLS34_06875"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HRP87225.1"
                     /translation="MYTASELATWRQELARLWDDSIPLAAAMGVEIRRLDNDVLELAA
                     PLAPNRNHMGSAFGGSLQGLATLAGWGVTLMAAGPPGSCHVVVRDAQMRFLIPVGGEL
                     VADAELPQATAVATFRMGLKERGRARLTVPVAIRGPGESPAARFVGEFVAFVRQP"
     gene            7051..7977
                     /locus_tag="PLS34_06880"
     CDS             7051..7977
                     /locus_tag="PLS34_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRP87226.1"
                     /translation="MKSALEAFKAIAEPSRLRLLALCARGELTVTEVTEVVGQSQPRV
                     SRHLKVLCAAGLLDRFREQNWVYYRVPLGGRGAELARGLLRLLPQDDPVLRLDRERLE
                     RVLARRAEQVRGRALGDAVPTPAGVDARIVDLMAGEALGDLLDIGTGTGRMLRLLGVG
                     ADTAVGIDISREMLVLARSNLHAAGLDHLSVRQGNMYQLRFADGSFDTVTIDQVLYQA
                     EQPAEVLREAARVLRPGGRLLLVEFLTGQAPVALAAAGANRDAQLTPTGLARWCEQVG
                     LLPEAAEELTARPWPLIVSIARRAEDRRAVAA"
     gene            8050..>8327
                     /locus_tag="PLS34_06885"
     CDS             8050..>8327
                     /locus_tag="PLS34_06885"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRP87227.1"
                     /translation="MEARLWQTVERLAPLRPEFVSVTYGADGSTRERTHEIVTRIHAE
                     TGLVSAPHLTCVGASRGEVLDVARRYRDAGIRHIVALRGDPPKGSDRYV"
BASE COUNT         1351 a         2781 c         2892 g         1303 t
ORIGIN      
        1 caaatcgcga ttctcgagca ccaggcggtt gcgctcagac agcttctccc ttgcccaacc
       61 gaaactggtc gagggcatgt cgaccagcac ccggatggga tagaccgcca ccgacagtgc
      121 ccggcgtacg gtctcgaggt gcccctggcg atggtcgagg accatcaggg caactgacag
      181 aaccgccagc agcaccatgc gggtgccgag cgagggtccg cgctcgaaca gcggcttgtt
      241 ctcggtctgg ctgagtgggc tcacggctag gtcacggcca accgctgcct attgtggacc
      301 gcgggcgggt ggcgaggcca gcgcctcact cgatgccgaa caggcccggc ccgtgctggt
      361 cgagcagctc gagcaccttg ccgccgccca gcgccacaca ggtgagcggc tcgtccgcga
      421 tgatgacggg caacccggtc tcctccatca ggagcttgtc gaggtcgcgc aacagcgccc
      481 cgccgccggt cagcacgatg ccgcgatccg cgacgtcggc gcccagctcg ggcggcgtct
      541 gttcgagcgc cgccttgacc gccccgacga tgccttgcag cggttcctgc agggcctcga
      601 gaatctcgtt ggagttcagc gtgaagctgc gcggtacgcc ttccgagagg ttgcggccgc
      661 ggacggaaat ctcgcgcact tcctggccgg gataggccgc gccgatctcg tgcttgatgc
      721 gctccgcggt ggcctcgccg atgaggatgc cgtagttgcg gcgcacatag ctcatgatcg
      781 cctcgtcaaa gcggtcgccg ccgatgcgca ccgaggccgc gtagacgatg ccgttgagcg
      841 agatgaccgc cacctcggag gtgccgccgc cgatgtcgag caccatcgag ccgcgcgcct
      901 cctgcaccgg catgccggcg ccgatcgcgg ccgccatcgg ctcctccacc aggtagacct
      961 tgcgtgcgcc ggcgccctcg gccgactcgc ggatggcgcg gcgctcgacc tgcgtcgagc
     1021 cgtagggcac gcagaccagc acacgcgggc tcggccggaa gtaacggctg ccgtgcgcct
     1081 tcttgatgaa gtgctgcagc atcttctcgg taacggtgaa atccgcgatc acgccgtcct
     1141 tgagggggcg gatggcggtg atattgccgg gcgtacggcc cagcatgctc ttggcttcgg
     1201 cgccgacggc ctgcacggtt cgcccgccgc gggaggattc ctcgcggatg gccaccacgg
     1261 agggctcgtt gagcacgatg ccgcggcccc ggacgtagat cagggtgttg gccgtgccca
     1321 ggtcgatgga caggtcgttg gagaaataac cccggatatt cttgaacata cgttgtcttg
     1381 gcagggatca ggcggcgact ggggggcggc gcttctggca ccgggcgggt tcggcggaca
     1441 agctcataaa tctagcaacg ggggcgactt cgggcaagcg cgagggtatc atcgatggct
     1501 tgtccaaggt gcgttttctt tgcgtcccag gaggcctgca tgaagcttag ccgacacgag
     1561 gtcgagtcca tcgcacacct cgcgaggctg gagatctcgg ccgacgagat ccccgtgtac
     1621 gtcgacaacc tttcccggat catcgacttc gtccagcagc tggagcgggc cgataccgag
     1681 ggcgtgacgc cgatggccca cccgctcaac atgacccagc cgctccgccc ggacgaggtc
     1741 accgagtcgg accagcacgc actctaccag cagaacgccg gggccgtgga atccgggttg
     1801 tacctggtcc cccgcgtcat cgaatagcca gggagcacgc acgcatgcac ggcatgacac
     1861 tggccgggat tgcccgcgcc ctgcgcgccc gcgaattcag cagcgaggaa ctcgtcagcc
     1921 acctgctgga gcggatcggc cgcctcgacg gccagctcaa tgccttcgtc accgtgacgg
     1981 gcgaggccgc gctgcaggcg gcccgggacg ccgaccggtt gctggccgag ggccgtgccg
     2041 cccccctcgc cggcgtaccg atcgcccaca aggacatctt ctgcacggcc ggcgtgaaga
     2101 ccagctgcgg gtcgcggatg ctggacaatt tcgtgtcgcc ctatgacgcc accgtggtgg
     2161 agcggctgcg ggccgccggc accatcatgc tgggcaagac caacatggac gagttcgcca
     2221 tgggctcgtc caacgagacc agcttctacg ggccggtgcg caatccctgg gatctcgagc
     2281 gagtgccggg tggttcctcc gggggctcgg ccgcggcggt cgcagcgcgg ctcgtgccgg
     2341 cggcgaccgg cacggatacc ggcggttcga tccgccagcc ggcggcgctc gccggcatca
     2401 ccggcatcaa gcccacctac ggtcgggtgt cgcgctacgg catgatcgcc tttgcctcca
     2461 gcctggacca ggccggcgtg ctgacgcaga ccgccgagga cgccgcgctg ctgcttggcg
     2521 cgatgtgcgg ccacgatccg cgcgattcca cctcggtcga cgcgcccgtg ccggactacg
     2581 cggcggggct cggggcgtcg ctcgagggac tccggatcgg actggtggag gaattcctgg
     2641 acgacggcct ggatgcggcc aacgcggcgg cggtcgaggc ggccctgaaa gtgctggagc
     2701 ggctcggcgc cgaggtgcgt cgagtcagcc tgcccaacat cggcctgtcg gtgccgacct
     2761 attacgtagt ggcgccggcg gagtgctcgt ccaacctgtc gcgcttcgac ggcgtccgct
     2821 tcggctaccg ggccgcggcg ccgcgcgacc tcgaggatct ctacaagcgc agccgcggcg
     2881 aggggttcgg cgccgaggtg aagcgccgca tcatgaccgg cacttacgtg ctgtcggccg
     2941 gctactacga cgcctactac ctgaaggcgc agcgctcgcg gcagctcatt gcggccgact
     3001 tccggcgcgc cttcgagcaa gtggacgtga tcgtgggtcc gacctcgccg acgccggcct
     3061 tcgagctcgg cgccaaggtg gacgacccgg tcaccatgta cctgaacgac atctacacca
     3121 tcggcgtgaa cctggcagga ctgccgggcc tgtccgtgcc ctgcggcttc atcgacggac
     3181 tgccggtcgg cctgcagatc attggtcgcg ctttcgacga aggacgcatc ctgaacgtcg
     3241 cacacggcta ccagcaggaa acggattggc acacgagggc gcccggcggc gctttcgcct
     3301 gaggaccgcg catggaatgg gaaaccgtca tcgggctcga gattcacgcc cagctcgcca
     3361 cccggagcaa aatcttctcc ggcgccgcca ccgcctacgg cgcggcgccc aacacgcagg
     3421 cctgcctggt cgatctcggc tatccgggcg tcttgccggt actcaacgag ggtgtagtca
     3481 ggatggccgt ggccttcggc ctcgccaccg agtcgaccat cgcggcgcgc tcggtgtttg
     3541 cgcgcaagaa ctacttctac cccgacctgc cgaagggcta ccagatcagc cagtacgaac
     3601 tgcccatcgt gcatgacggc cgcatcgagc tgcagctcga cgacggtgtc cacaagacgg
     3661 tcggcatcac gcgcgcgcac ctggaggaag acgccggcaa gtcgctgcac gaggacttcc
     3721 agggccagtc gggcatcgac ctgaaccggg ccggcacgcc gctgctggaa atcgtctccg
     3781 agccggacat gcgctcgacc aaggaggcgg tcgcctacat gaaggcgatc caccagctgg
     3841 tgcgttacct cggcatttgc gacggtaaca tgcaggaagg ctcgtttcgt tgcgacgcca
     3901 acgtctcggt gcggccgaag ggccggcgcg agttcgggac tcgcaccgag atcaagaacc
     3961 tgaactcctt ccgttatgtg gagcgcgcca tcctgttcga ggtggaacgc cagatcgaca
     4021 tcctcgagtc cggcggcagc gtggtgcagg aaacgcggct ctacgacccg gaccgggacg
     4081 agacccggcc gatgcgcagc aaggaagagg ccaacgacta ccgttatttc cccgatcccg
     4141 acctgctccc gctggaagtg gggccggagc tggtcgaggc ggtgcgtgcc gcgctgccgg
     4201 agctgccgtg gcgcaagcgg gaccgcttca tggacgagta cggactttcg gagtacgacg
     4261 cgggcgtgct ctgcgcgagt cgcgcgggcg ccgactattt cgaggcggtg gccgccggcg
     4321 tcgcggacaa gaaactcgcc gccaactggg tgatgggcga gctggccggg gcgctcaaca
     4381 aggcaggcct ggaggtcacg gcgagcccgg tgcccgcagg cgcactgctg ggcctgctta
     4441 aacgtatcga ggacagcacc atttccggca agatcgccaa ggaagtcttc gaggcgatgt
     4501 gggccggcga gggcgatgcg gacgccgtga tcgaggcgcg cgggctcagg cagatcaccg
     4561 actccgactc ggtggaggcg gtgattcgcg aggtcatggc cgccaacccg cagcagctcg
     4621 agcaatatcg ctccggcaag gacaagctgt tcggcttttt cgtcgggcag gtgatgaagg
     4681 ccaccggtgg caaggccaat ccggcgctgg tcaacgagct gctcacgaaa ctgctggcgg
     4741 gctgatgagc ggcggcggcg acgcgctgcg gcgcttcatg ttcgaggact ttcccgtgcg
     4801 gggcgaaatc gtgcgcatgg acacgagctg gcgtgcggtg ctcgagcggc aggactaccc
     4861 accggcggtc cgggacctgc tgggcgaagc catggctgcc acggtgctgc tggcctcgac
     4921 gctgaagttc gacggcatgc tcaccctgca gatccagggc gagggcgacc tgcacctgct
     4981 ggtcgcgcaa tgcggcagcg acctcgccgt gcgcgggctc gccaagtgga agagcgacga
     5041 tccacgcggc acgctcgctg agctcaccgg cggcggacgg ctcgccatca ccattgaaag
     5101 gcgcaaggac cgcgagcgct accagggtat cgtgctggcg gacaccgata cgctggcggg
     5161 ctgcctggaa acctatttcg cacaatcgga gcagttgccg acccgcatct ggctggcggc
     5221 cgacgacgag cgcgcggccg gcatgctctt gcagcaaatg ccgtccgaag ccgaagagac
     5281 ggatcgggac ggctggaacc gcgccggcct gctggccgaa acgctatcgt ccgacgagtt
     5341 gctcaagctc gacgcgcgcg acgtgctgcg ccgactcttt cacgaggaga acctccggct
     5401 cgccgaccag cgtggggtgg cgttccactg cagttgcaca cggcagcggg tggaatcggc
     5461 gatgcgcctg ctgggacgcg acgagctggc cgaactgctg cgggcggacg gccgcatcga
     5521 ggtgcgctgc gaattctgca acaaggccta cgggctggat gcagtggacg tggaacagat
     5581 ccttgccgat gccgggccac tccagccgcc gggctctgaa cgcgttcact gactacgcgg
     5641 ggacgtgcgg tccgtccagg tcaagcgcct gcagtcgcac cgtgtccgcc cagtcgagtt
     5701 catggtgatc gaagccgcgc gccagctttg gcttgacgac ttcgaagtag ggcgacacgt
     5761 cgaaatcccg cggggcgtac aagctgtgat gacggatgtg cagccactcg cgatactggg
     5821 ctgagtcggt cggcaggtcc tgcaggtaca gctcgggcag gatgggatag ccgatcgacc
     5881 cgaaggcctg tgcgatcagc gacgagcaga tggcgcgggt cgggtcgccg ctgccgagcg
     5941 cgaggagctt gcggcgccag cgtcgcggca ccgggggcgt cggcatcagg tagcgcgcca
     6001 ggtcccagat gttcttgaga tcgtactggt gaccgatgcg cgccacgacg aacgccacca
     6061 gttcatcgag ctctcgcgac gacagcccga ccggccggca gatacgggtg tgcaggtccc
     6121 agtactccga cagcggcagg gcgcgcacgc ctgtattgat gtccgcctcg accagcaaca
     6181 gggactcacc atcctgtgac cggcggccga gggcatcacc cacgcacagc gcagcgtggg
     6241 accaggtgga ctgggtcagg tacttgactg cagaagcgaa caggctggtg ccttccacca
     6301 gcagcacgtc ccccctttgc agcgtggcgg ccaggttttc ccggctgccg gtcgcaacgc
     6361 ggcccccgtt ttcgcgagga cgcgaaaggt aggcggcgat acggcgccca agggtgttga
     6421 ggaggcgcat gtcgcggacg ctagcggtac caggcccccc ggcgccgcga actggttcag
     6481 gtttttacgg ttccggcggg gccggccgcc ggctcatggc tggcgaacaa aggccacgaa
     6541 ttcgccgacg aagcgtgctg ccggcgactc gcccggcccg cggatggcga ccggcaccgt
     6601 caatcgcgca cgaccacgtt ccttcagtcc catgcggaaa gtcgccaccg cggtggcctg
     6661 gggcaattcg gcatcggcaa cgagttcgcc gccgacgggg atgagaaatc gcatctgcgc
     6721 gtcccgcacc accacgtgac aggaccccgg cggccccgcc gccatcagcg tcacccccca
     6781 gccggccagc gtggcgagcc cctgcaggct gccgccgaag gcggatccca tatggttgcg
     6841 gttcggcgcc agcggtgccg cgagttccag cacgtcgttg tccaggcgac ggatttccac
     6901 gcccatcgcc gccgccagcg gaatgctgtc gtcccagagc cgggccagtt cctgtcgcca
     6961 cgtggcgagc tccgatgcgg tatacatgtt gtcacctata taaagatatg tttatattca
     7021 gcctgagtca tttttgcaac tgaggtccct gtgaagtccg cgctcgaagc cttcaaggcc
     7081 atcgccgaac ccagccgtct ccggctgctg gcgctgtgcg ctcgtggcga gcttaccgtc
     7141 accgaggtca ccgaggtggt cgggcagagc cagccacggg tgtcccggca cctcaaggtg
     7201 ctgtgtgcgg caggactgct ggatcgtttt cgcgagcaga actgggtgta ctaccgcgtg
     7261 ccgctgggcg ggcgcggggc ggagctggcg cgtggtctgc tgcggctgct gccgcaggac
     7321 gacccggtgc tgcggctgga ccgcgagcgg ctggagcgcg tgctggcgcg ccgggccgaa
     7381 caggtacgcg gccgtgcgct cggtgacgcg gtgccgaccc cggcgggcgt ggacgcgcgc
     7441 atcgtcgacc tgatggcagg cgaagcgctc ggcgacctgc tcgatatcgg taccggcacg
     7501 gggcgcatgt tgcggttgct cggggtcggc gccgacacgg cggtcggcat cgacatctcg
     7561 cgcgagatgc tggtgctggc gcgcagcaac ctgcatgcgg ccgggctgga tcatctctcc
     7621 gtgcgccagg gcaacatgta ccagctgcgc ttcgccgacg gcagcttcga caccgtgacc
     7681 atcgaccagg tcctgtacca ggccgaacag ccggccgagg tgctgcgcga ggcggcgcgc
     7741 gtgttgcggc cgggcggacg cctgctgctg gtggaattcc tgaccggcca ggcgccggtc
     7801 gccctggctg ctgccggcgc gaaccgggat gcgcagctca ccccgacggg gctcgcccgt
     7861 tggtgcgagc aggtcgggct gctgcccgag gccgccgagg aactcacggc gcgtccatgg
     7921 ccattgatcg tttccatcgc ccgccgcgcc gaggaccggc gtgccgtggc cgcttgaacc
     7981 gggagtcctt gcttgaaccg ccatcccatc cgcttttcct tcgagttctt cccgccacgg
     8041 gacgccggca tggaggcgcg cctgtggcag accgtggagc ggcttgcgcc gctcaggccc
     8101 gagttcgtct cggtgaccta cggcgccgac ggctcgacgc gcgagcgcac ccacgagatc
     8161 gtcacgcgca tccacgcgga aaccggcctg gtgagtgcgc cgcacctgac ctgcgtcggc
     8221 gccagccgcg gcgaggtgct ggatgttgct cgacggtatc gcgacgcagg catccggcac
     8281 atcgtcgcat tgcgcggcga cccgccgaag ggcagcgatc ggtacgt
//
LOCUS       DAOMPG010000099         8282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3711_length_8282_cov_4.497630, whole genome shotgun sequence.
ACCESSION   DAOMPG010000099 DAOMPG010000000
VERSION     DAOMPG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3711_length_8282_cov_4.497630"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="ffh"
                     /locus_tag="PLS34_06890"
     CDS             <1..1241
                     /gene="ffh"
                     /locus_tag="PLS34_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRP87228.1"
                     /translation="LEADVALPVVKDFIERTRARALGAEVAKSLTPGQAFIKIIHDEL
                     VRTMGSANEALNLRAQPPAIVMLAGLQGAGKTTTAAKLARRLAERDKRKVMLVSADVY
                     RPAAILQLERLAGEVGAVFCPSDAGEDPVAIAQRAVEAARRELCDVLIIDTAGRLHVD
                     EGMMAEAGRIHAAVNPVETLFVVDSMAGQDAVNAARAFGAALALTGVILTKTDGDARG
                     GVALSVRQVTGKPIKFIGAGEKTTALEPFHPERLASRILGMGDVVSLVEEVQHKVDRD
                     QADKLARKLSKGQGFNLQDLRDQIRQMQQMGGLAGLLDKLPGNAMPARVDSKTGEREM
                     RRQAAVIDSMTPKERRHPAIIDGSRKRRIAAGAGVQVQEVNRLLKQHAQMQKMMKKFS
                     KGGMARMMRTMKGRLPPGLG"
     gene            1383..1634
                     /gene="rpsP"
                     /locus_tag="PLS34_06895"
     CDS             1383..1634
                     /gene="rpsP"
                     /locus_tag="PLS34_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRP87229.1"
                     /translation="MVTIRLTRGGAKKRPFYHVVVTDSRNRRDGRYIERLGFFNPIAT
                     GKEEGLRLDLERVNYWLGHGAQPSERVASLIKQFEKKAA"
     gene            1673..2179
                     /gene="rimM"
                     /locus_tag="PLS34_06900"
     CDS             1673..2179
                     /gene="rimM"
                     /locus_tag="PLS34_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502682.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRP87230.1"
                     /translation="MTGQRRVVLGKVGGLYGVRGWVRLWSFTDPTDNLLDYRDLELGR
                     GGRWESVRLAEGRVQGESLVVRFEGCADRDQAALLVGAELAVARERLPPAAEGEYYWA
                     DLVGLEVVTTGGVALGRVDHMMATGANDVMVVVGERERLLPFVPGQYVSEVDLAGGRI
                     VVDWDPEF"
     gene            2190..2942
                     /gene="trmD"
                     /locus_tag="PLS34_06905"
     CDS             2190..2942
                     /gene="trmD"
                     /locus_tag="PLS34_06905"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRP87231.1"
                     /translation="MHFEVVTLFPELVGLVGRFGVVGRAVERGRLRLGTTDPREFATD
                     VHRTVDDRPYGGGPGMVMKVEPLRSAIRAARAKLPAGSRTVFLSPQGRVFSQAVAGEY
                     RNLPGLLLVAGRYEGIDERVVRMEADETLTIGDYVLSGGELPAMLVVDAVARLVPGVL
                     GDENSALEDSFSKGLLDCPHYTRPEVVEGLRVPPVLLSGDHEAIRRWRLAQSLGRTWT
                     IRPELLAGRELSAEERQLLDEYIAARATGGTD"
     gene            2974..3318
                     /gene="rplS"
                     /locus_tag="PLS34_06910"
     CDS             2974..3318
                     /gene="rplS"
                     /locus_tag="PLS34_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRP87232.1"
                     /translation="MSNIIEELDKEQMQREVPDFSPGDTVVVQVKVKEGNRERLQAFE
                     GVCIARRNRGLNSAFTVRKISHGEGVERVFQTYSPAIAEIQVKRRGDVRRAKLYYLRD
                     RTGKAARIKEKV"
     gene            3322..5175
                     /gene="sppA"
                     /locus_tag="PLS34_06915"
     CDS             3322..5175
                     /gene="sppA"
                     /locus_tag="PLS34_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRP87233.1"
                     /translation="MIWRGLRAFFRSVWRTLDGVRKAMHLLLMLLVLLGIVLALASRP
                     HKLPEAFTLVVSPAGLLVEQYSGDAVSRALEAAQGLPRAETLVSDLVEAIDEAAADSR
                     VKAIQLEVDGLAGGSMDKLATIAAALHRFSETGKPVVAYTAFVPMRNYFLAAHADELY
                     LDPHGIVLLQGFGFYRHYYKSAMEKLSLDWYVFSAGEAKSFADPYRRDDMSPAERENL
                     GPIASGMWDAWRDGVAQARGLEPALLDDYIERFLPRLKAAGGDTARAALDAGLVDGLR
                     SFDEVEARLVDAGGHDREGEYVGVHSEEYLAAVRAKRAARSTSRGKQAPAVGLIVARG
                     EILTGDQPPGSIGDESMRTLIREARDDDNVRALVLRIDSGGGSVMASEAIVRELDRVR
                     EAGKPVIVSMGGVAASGGYMIALPADEIWAHPTTITGSIGVIGMFPNFGRLLDRLGVS
                     LDGVGTHRYSGDFRLDREFSPEVMEIIQAIVDGTYDKFIGEVADWRDLPVAEVRGLAE
                     GRVWLGEVALESGLVDRLGSLDDALSSAADHAGLDEDYEVVLIEQELGFGERMMVELL
                     SRAGRIGFTAWPRSLLERLPVEIRTLFTELERMDGFADPRGIYYHCLCDAW"
     gene            complement(5188..6213)
                     /gene="tdh"
                     /locus_tag="PLS34_06920"
     CDS             complement(5188..6213)
                     /gene="tdh"
                     /locus_tag="PLS34_06920"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRP87234.1"
                     /translation="MRALVKAKAEPGIWMQDIRRPEPGPNDVLIRIRKTAICGTDMHI
                     YNWDEWAARTIPVPMAVGHEFVGQVEAVGSEVQGIAPGDRVSGEGHLTCGHCRNCRAG
                     RRHLCRNTIGVGVNRQGCFAEYLCIPASNVFKLPPAISDEIASILDPLGNAVHTALSF
                     DLVGEDVLITGAGPIGIMATAIARHVGARHVVITDVNDYRLDLAARMGATRTVNVTRQ
                     KLDDVMRELDMQEGFDVGLEMSGNPQAFRDMLRTMHHGGGVALLGIPPEETAIDWNQV
                     IFKGLTIKGIYGREMFETWYKMASMLQSGLDISPIITHRFKAEDYLEGFRTMGSGQSG
                     KVILDWA"
     gene            complement(6268..7458)
                     /gene="kbl"
                     /locus_tag="PLS34_06925"
     CDS             complement(6268..7458)
                     /gene="kbl"
                     /locus_tag="PLS34_06925"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRP87235.1"
                     /translation="MNQDLLERLGTELSALREQGLYKSERVIDSPQQAAIHMQGGREV
                     INLCANNYLGLANHPALIAAAHEALDRYGYGMASVRFICGTQTPHKELEKRISAFLGM
                     EDTILYPSCFDANGGLFETLLGEEDAVISDQLNHASIIDGIRLSKARRYRYRHNDMAD
                     LEAQLESAADARVRLIATDGVFSMDGTIAELAKICDLADRHDALVMIDDCHATGFLGA
                     TGRGTHERHGVLGRVDIITGTLGKALGGASGGFVAARGPIVEWLRQRSRPYLFSNTVA
                     PVIAATSIKVLDLLESGAELRRRLWDNTHFFRERMAALGFDLKPGAHPIIPVMLGDAK
                     LATTMADRLLDEGVYVIGFSFPVVPHGQARIRTQMSAGHSTHHLERAIAAFEKVGRDL
                     GVIR"
     gene            7540..7929
                     /locus_tag="PLS34_06930"
     CDS             7540..7929
                     /locus_tag="PLS34_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRP87236.1"
                     /translation="MEQDEVRLDRWLWAARFFKTRAVAQQAIAGGLVHHNGQRVKAAR
                     AVSPGDRLEITRGEERFEIQVLALAARRGPATVARGLYEETEASLAARSAAQQARRDT
                     AGNRPPASRPDKRDRRRIISFTRSCED"
     gene            7960..>8282
                     /locus_tag="PLS34_06935"
     CDS             7960..>8282
                     /locus_tag="PLS34_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87237.1"
                     /translation="MKPLIAALLLSCWIVLGSTAAAAPAPHLQGALPGIEATLVEMHG
                     AAQRERVRRGLRQAAELWRPDDGDAEAFAEFARTHFAGDQATLDAMFDRYQYLLERLG
                     GHMNKL"
BASE COUNT         1315 a         2605 c         3050 g         1312 t
ORIGIN      
        1 tgctcgaggc cgacgtcgcc ctgccggtgg tgaaggattt catcgagcgc acccgggccc
       61 gggcgttggg cgccgaggtg gcgaagagcc tgaccccggg gcaggccttc atcaagatca
      121 tccatgacga gctggtgcgc accatgggtt cggccaacga ggcgctgaac ctgcgcgccc
      181 aaccgccggc gatcgtgatg ctggcgggcc tgcagggcgc cggcaagacc accaccgcgg
      241 ccaagctggc gcggcgcctg gccgagcgcg acaagcgcaa ggtgatgcta gtgagtgccg
      301 acgtctaccg gccggccgcc atcctgcagc tcgagcggct ggcgggcgag gtgggcgcgg
      361 tgttctgtcc cagcgacgcc ggcgaggatc ccgtcgccat cgcgcagcgt gcggtcgagg
      421 ccgcgcggcg cgagctgtgc gacgtgctca tcatcgacac ggcgggacgc ctgcacgtcg
      481 acgaagggat gatggccgag gcgggccgca ttcacgccgc cgtgaacccg gtcgagacgc
      541 tgttcgtggt cgacagcatg gccggccagg acgcggtcaa cgccgcccgg gccttcggcg
      601 cggcgctggc actgaccggc gtgatcctca ccaagacgga cggcgatgcg cgcggcggcg
      661 tggcgctgtc ggttcgccag gtgaccggca agcccatcaa gttcatcggc gccggcgaaa
      721 agaccacggc gctggagccg tttcatccgg agcgcctggc gtcccggatc ctgggcatgg
      781 gcgacgtggt gtcgctggtg gaagaggtcc agcacaaggt ggaccgcgac caggccgaca
      841 agctggcacg caaactgtcc aagggccagg gcttcaacct gcaggatctg cgcgaccaga
      901 ttcgccagat gcaacagatg ggcgggctcg cggggctgct cgacaagctg cccggcaatg
      961 ccatgccggc cagggtggat tcgaagacgg gcgagcgcga aatgcgccgc caggccgcgg
     1021 tgatcgactc gatgacgccg aaggagcggc gccacccggc aatcatcgac ggctccagga
     1081 agcggcgcat cgccgccggg gcgggggtcc aggtccagga ggtcaaccgc ctgctgaagc
     1141 agcacgcgca gatgcagaag atgatgaaga agttcagcaa gggcggcatg gccaggatga
     1201 tgcgcaccat gaagggccgc ctgccgccag gcctcggctg acggccaggc cgcggggccc
     1261 ttcccattgg gccagattcc agtaaaatgc ccggtttacc tgcggccgcc ggagggtggt
     1321 cgcgccgtgg cgcgtggctg gtttgcgcgc cacgtccagc acgaacgagg aaatccggac
     1381 tcatggtgac gattcgtctg acgcggggcg gcgcgaagaa gcggcccttc taccacgtgg
     1441 tggtgaccga cagccgcaac cggcgcgacg gtcgctacat cgagcggctg ggctttttca
     1501 acccgatcgc gacgggcaag gaagagggcc tgcggcttga cctcgagcgc gtaaactact
     1561 ggctcgggca cggcgcgcag ccgtccgagc gggttgcgag cctgatcaag cagttcgaga
     1621 agaaggcggc ctgaggcttt ccgcggcgcg tccgcgcgcc ggggcgggga tcatgaccgg
     1681 tcaacgacgg gtggtcctgg gcaaggtcgg cggcctctac ggggtgcgtg gctgggtccg
     1741 gctctggtcg ttcaccgacc cgaccgacaa cctgctcgac taccgggatc tcgaactggg
     1801 ccggggcgga cgctgggaat cggtccggct ggcggagggc cgggtacagg gcgagtcgct
     1861 ggtggtgcgc ttcgagggct gcgcggatcg cgaccaggcg gcgttgctgg tgggcgcgga
     1921 actggcggtc gccagggagc gcttgccgcc ggcggcggag ggcgagtact actgggccga
     1981 cctggtcgga ctggaagtgg tgacgaccgg cggcgtggcg ctgggccggg tcgatcacat
     2041 gatggccacc ggggcgaacg acgtcatggt ggtggtgggc gaacgggagc gcttgctgcc
     2101 cttcgtgccg gggcagtacg tgagcgaagt ggacctggcc ggcgggcgta tcgtcgtcga
     2161 ctgggacccg gaattttgag gaaggcgtca tgcacttcga ggtcgtgacg ctgtttccgg
     2221 agctggtcgg cctggtcggc cgcttcggag tggtggggcg cgccgtggag cgcggacggt
     2281 tgcggctcgg caccaccgat ccgcgggagt tcgccacgga cgtgcaccgc accgtggacg
     2341 accggcccta tggcggtggt ccggggatgg tgatgaaagt ggagccgctg cgcagcgcca
     2401 tccgcgctgc cagggcgaag ctgccggcgg gcagccggac ggtgttcctg tcgccgcagg
     2461 ggcgggtatt cagccaggcg gtggccgggg aataccggaa cctgccgggg ctgctgctgg
     2521 tggcggggcg ctacgaggga attgacgagc gggtggtccg catggaggcg gacgagacgc
     2581 tgacgatcgg cgactacgtg ttgagcgggg gcgaactccc tgcgatgctg gtggtcgatg
     2641 cagtcgcgcg gctggtgccg ggcgtgctgg gcgacgagaa ttccgcgctg gaggactcgt
     2701 tcagcaaggg gctgctggac tgcccgcact acacgcggcc cgaggtggtc gagggcttgc
     2761 gggtgccgcc ggtgctgctg tcgggcgacc acgaggcgat caggcgctgg cgcctggcgc
     2821 agtcgctggg acggacatgg acgatacggc cggaactgct ggccggacgg gagctgagcg
     2881 ccgaagagcg gcagctgctg gatgaataca tcgccgcacg ggcaaccggc ggcacggact
     2941 gaaactggga tagcttgggc gcgggtgagc gatatgagca acatcatcga agaactggac
     3001 aaagagcaga tgcagcgcga agtccctgac ttttcgcccg gcgataccgt cgtcgtgcag
     3061 gtcaaggtca aggaaggcaa ccgtgagcgc ctgcaggcgt tcgaaggtgt gtgcatcgcg
     3121 cggcgcaatc gcgggctgaa ttccgcgttc accgtccgca agatctcgca cggcgagggg
     3181 gtcgagcggg tcttccagac ctacagcccg gcgatcgccg agatccaggt caagcgccgt
     3241 ggcgacgtgc gtcgcgccaa gctctactac ctgcgtgatc gcaccggcaa ggccgcgcgc
     3301 atcaaggaaa aggtctagtc cttgatctgg cgaggcctgc gggccttttt caggtcggtc
     3361 tggcggaccc tggacggggt ccgcaaggcg atgcacctgt tgctgatgct gctggtgctg
     3421 ctcggcatcg tgctggcgct cgcgtcgcgc ccgcacaagc tgccggaggc ctttaccctg
     3481 gtcgtcagtc ccgctgggct gctggtggag cagtactcgg gcgacgcggt cagcagggcg
     3541 ctggaggccg cccagggcct gccgcgcgcc gagaccctgg tcagcgacct ggtcgaggcc
     3601 atcgatgagg ccgccgcaga ctcgcgcgta aaagccatcc agctcgaggt cgacgggctc
     3661 gctggcggca gcatggacaa gctggcgacg atcgccgcag cgctgcaccg cttcagcgaa
     3721 accggcaaac cggtggtcgc gtataccgcc tttgtgccca tgcgaaacta ctttctcgcg
     3781 gcgcacgcgg acgagctcta tctcgatccg cacggtatcg tgctgctgca gggctttggc
     3841 ttctaccgcc actattacaa gagcgcgatg gagaagctct cgctggactg gtacgtcttc
     3901 agtgccggcg aggcgaagag tttcgcggat ccttaccggc gcgacgacat gtcgcccgcg
     3961 gagcgggaaa acctggggcc catcgccagt ggcatgtggg acgcgtggcg cgacggcgtg
     4021 gcgcaggctc gcggactcga gccggcgctg ctcgatgatt acatcgagcg gttcctgccg
     4081 cgcctgaagg ctgccggcgg cgataccgcc agggcggcgc tcgacgccgg gctggtggac
     4141 gggctgcgca gcttcgacga ggtggaagcg cgcctggtcg atgccggagg gcacgaccgc
     4201 gagggcgagt atgtcggtgt ccacagcgag gaatacctgg cagccgtccg ggcgaaacgc
     4261 gcggcgcgca gcacgtcccg cggcaagcag gcgccagccg tcggcctgat cgtcgcacgc
     4321 ggcgagatcc ttacgggtga tcagccgccg gggtcgatcg gcgacgaatc gatgcgcaca
     4381 ttgattcgtg aagcccgcga cgacgacaac gtgcgcgcgc tggtgttgcg catcgacagc
     4441 ggcggcggca gcgtcatggc ctccgaggcc atcgtccggg agctcgaccg ggtgcgcgag
     4501 gccggcaagc cggtgatcgt gtcgatgggc ggcgtcgctg cgtcaggcgg ctacatgatc
     4561 gcgttgccgg cggacgagat ctgggcgcac ccgaccacga tcacgggctc catcggcgtc
     4621 atcggcatgt tccccaactt cggccgcctg ctggaccggc tgggtgtcag cctcgacggc
     4681 gtcggcaccc atcgatacag cggcgatttc cggctggacc gtgagttcag cccggaggtc
     4741 atggagatta tccaggcgat cgtcgacggc acctacgaca agttcattgg ggaagtggcc
     4801 gattggcgcg acctgcccgt ggctgaagtg cgtgggctcg cggagggacg cgtgtggctc
     4861 ggcgaggtcg ccctcgagtc cggcctcgtg gaccggctcg gctcgctgga cgacgcgctt
     4921 tcgtcggccg ccgatcacgc cggactcgac gaggattacg aggtcgtgct gatcgagcag
     4981 gaattgggct tcggcgagcg catgatggtc gagctgctca gccgtgccgg gcggatcggc
     5041 ttcaccgcat ggccgcgctc gctgctggaa cgcctgccgg tggagattcg cacgctgttc
     5101 accgagctcg agcgcatgga cggcttcgcc gacccgcgcg gcatctacta ccactgcctc
     5161 tgcgacgcct ggtagaaaac cgggctttca ggcccagtcg aggatgacct tgccggactg
     5221 gccggaaccc atggtgcgaa agccctcgag gtagtcctcg gccttgaagc gatgggtgat
     5281 gatggggctg atatccagcc cgctctgcag catgctggcc atcttgtacc aggtctcgaa
     5341 catctcgcgg ccgtagatgc ccttgatcgt cagtcccttg aagatgacct ggttccagtc
     5401 gatggccgtc tcctccggtg gtatgcccag cagagcgacg ccgccgccgt ggtgcatggt
     5461 gcgcagcatg tcgcggaagg cctgcgggtt gccggacatc tccagcccga catcgaaacc
     5521 ttcctgcatg tccagttcgc gcatgacgtc gtccagcttc tggcgcgtca cgttgacggt
     5581 gcgcgtggcg cccattctcg cagccaggtc gagccggtag tcattgacat ccgtgatgac
     5641 gacgtgtcgc gcaccgacgt ggcgcgcaat cgccgtcgcc atgatgccga tcgggccggc
     5701 gccggtgatg agtacgtcct cgccgaccag gtcgaatgac agcgcggtgt gcaccgcgtt
     5761 gccgagcggg tcgagaatcg aggcgatctc gtcgctgatg gccggcggca gcttgaagac
     5821 gttgctcgcc ggaatgcaga ggtactccgc gaagcagccc tggcggttga ccccgacgcc
     5881 gatggtgttg cggcacaggt gccggcggcc ggcgcggcag ttgcggcaat ggccgcaggt
     5941 gaggtggcct tcgccggaca cgcgatcgcc gggggcgatc ccctggactt cgctgccgac
     6001 agcctctacc tggccgacga attcatggcc caccgccatc ggcaccggaa tggtcctggc
     6061 cgcccattcg tcccagttgt agatgtgcat gtccgtcccg cagatcgccg tcttgcggat
     6121 gcggatcagg acatcgttcg gcccgggctc gggacgacgg atatcctgca tccagattcc
     6181 cggttcggcc ttggccttga cgagcgcgcg catggtgaac tccgattcgc tgtgcctggg
     6241 tgacgtgacg gattcgcggg gcgggcctca gcggatgacc ccgaggtccc gcccgacttt
     6301 ctcgaaggcg gcaatggccc gctcgaggtg gtgggtggag tggccggccg acatctgcgt
     6361 gcggatgcgc gcctggccgt gcggcacgac cggaaacgag aagccgatca cgtagacgcc
     6421 ctcgtcgagc agccggtcag ccatggtggt ggccagcttc gcatccccca gcatgaccgg
     6481 gatgatggga tgcgcgccgg gtttcaggtc gaaaccgagc gcggccatgc gctcgcggaa
     6541 gaaatgcgtg ttgtcccaca gccggcgccg cagctcggcg cccgactcga gcaggtccag
     6601 caccttgatc gaggtcgcgg cgatcaccgg cgccacggtg ttggagaaca ggtaggggcg
     6661 ggaacgctgc cgcaaccact cgacgatggg gccgcgcgcc gcgacgaagc cgccagaggc
     6721 gccgccgagc gccttgccga gcgtgccggt gatgatgtcg acgcgcccga gcacgccgtg
     6781 ccgctcgtgg gtcccccggc cggtagcgcc gaggaaaccg gtggcatggc agtcgtcgat
     6841 catcaccagc gcgtcgtgcc ggtcggccag gtcgcagatc ttcgccagct ccgcgatcgt
     6901 gccgtccatg gagaacacgc cgtccgtggc aatcagccgc acgcgcgcgt ccgccgcgga
     6961 ctccagctgg gcctcgaggt cggccatgtc gttgtggcga taacggtagc ggcgcgcctt
     7021 ggacagccgg atgccgtcga tgatcgaggc gtggttgagc tggtcgctga tgactgcatc
     7081 ctcttcgccc agcaaggtct cgaacagccc gccgttggcg tcgaagcacg aggggtagag
     7141 gatcgtgtcc tccatgccca ggaacgcgct gatgcgcttc tcaagttcct tgtgcggcgt
     7201 ctgcgtgccg cagatgaaac gcaccgaggc catgccgtag ccgtagcggt ccagggcctc
     7261 gtgcgctgca gcaatcagtg ccgggtggtt ggccagcccg aggtagttat tggcgcaaag
     7321 gttgatgacc tcgcggccac cctgcatgtg gatggcggcc tgctgcggcg agtcgatgac
     7381 gcgctcggac ttgtacaagc cctgttcccg gagggcgctg agctccgtgc cgaggcgttc
     7441 gagaaggtcc tggttcattg cggcgtcctg cagggtcgaa aggcggatta tagcaatgcg
     7501 gaacgctgca accggagcgt gctgcgctaa actgtgccga tggaacagga cgaggtccgg
     7561 ctcgaccgat ggctgtgggc ggcgcgtttt ttcaagacgc gcgccgtggc gcaacaggcc
     7621 attgccggcg ggctggtcca ccacaatggc cagcgcgtca aggcggcgcg ggcggtgagc
     7681 cccggcgacc ggctggagat cacccgcggc gaggagcgct tcgagattca ggttctcgcg
     7741 ctggctgcgc gacgcgggcc ggccacggtg gcgcgcgggc tctacgagga gaccgaggcc
     7801 agcctggcgg cgcggtcggc ggcgcagcag gcgcgccgcg acactgccgg caaccggccg
     7861 ccggcgtcgc gacccgacaa gcgcgaccgg cgccggatca tcagtttcac ccgatcctgc
     7921 gaagattgac tcatttttga tttgccctcg gagccgctta tgaagcccct gattgccgca
     7981 ttgctgttgt cctgctggat cgtcctcgga agtaccgcgg ccgccgcgcc cgcgccgcac
     8041 ctgcaagggg cgctgccggg gatcgaggcg acgctggtgg aaatgcacgg ggcagcgcag
     8101 cgcgagcgcg tccggcgcgg cctgcgccag gcggcggagc tctggcgccc ggacgacggc
     8161 gacgccgagg cctttgccga gttcgcccgc acgcatttcg ccggcgacca ggccacgctg
     8221 gacgccatgt tcgaccgcta ccagtacctg ctggagcggc tcggtggtca catgaacaag
     8281 ct
//
LOCUS       DAOMPG010000100         8237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3737_length_8237_cov_3.807504, whole genome shotgun sequence.
ACCESSION   DAOMPG010000100 DAOMPG010000000
VERSION     DAOMPG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_8237_cov_3.807504"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2160)
                     /locus_tag="PLS34_06940"
     CDS             complement(88..2160)
                     /locus_tag="PLS34_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP87238.1"
                     /translation="MSRGVSVNGRPVFATLLAGLAALLAALAALAAAGAPRGHVVVAG
                     SSTVFPFAAAVAERAPRPGPLVLPMGTGAGIAWFCEGLGPATPDVVLASRPMTPTEAS
                     ACRVAGVTPLDAVPVGRDGIVLASAPGHPIAALSLAELWLAVARRVPDPRGGSGWVPN
                     PHARWSEIRADLPDLPIRVLGPPATSGTRDALSSLALRPACEAALADSPRADAERRAC
                     GEIRRDGAWVDAGENDDVLAARLGSDPAALGVVGYGALLRNAGRVRGVCFDGACPTAA
                     SISAGDYPLARTLYLYFKPAHRGFVPGLEAFVAGFRAPDAVGVEGWLTRLGLVPLPPA
                     APAARPPHGTVPPPVAAEAGLSARVTAVVLAIVLVALLVGSSALRRAVPAQRALERGM
                     GLTLWSSAAVAGALLAVVLAALVVPAVNFFLQVSPREFLAGTHWSPESAIRAAQSVGE
                     GAFGVLPVLLGSVVVAGVALLLALPLALGAALHAHAWAGRRFRRAWRHGIRVAAFVPT
                     VIFGLFAALTVAPAVAAVAGAVGLAVAPGNLLAAGLVVGIMLLPVLALRIGDALESVP
                     TAAADAALGLGATRWEVLRDVVLPSAAGGIGAAVLLAVSRALGETVVVLMAAGLVAQF
                     TLDPLAANTTLTAQMVALMSGTHALENVRTQLPFVLGLLLAALVLPLNAWAFRLLRKS
                     DVSPSPVF"
     gene            complement(2138..2977)
                     /gene="pstB"
                     /locus_tag="PLS34_06945"
     CDS             complement(2138..2977)
                     /gene="pstB"
                     /locus_tag="PLS34_06945"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRP87239.1"
                     /translation="MNAVAEKQVLDTSVAGIEPTVFHVTRSPEGAALDVRNFNLWYGE
                     AQALYDVNMEIQKGLVTALIGPSGCGKSTLLRCLNRMNDLVDDLKISGEIIVNDENIC
                     RPAIDVIALRKRMGMVFQKPNPFAMSIYENVVYPLRVDGVSNKKVLNETCERALKGAA
                     LWNEVKDRLQESALGLSGGQQQRLCIARAIASDPEILLMDEPCSALDPIATAKIEELI
                     DELKGHYTIVIVTHNMQQAARVSDNTAFMYLGRLIEYGATDTVFTNPKAKRTSDYVTG
                     RFG"
     gene            complement(2999..4744)
                     /gene="pstA"
                     /locus_tag="PLS34_06950"
     CDS             complement(2999..4744)
                     /gene="pstA"
                     /locus_tag="PLS34_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRP87240.1"
                     /translation="MASTADTAAGAATEAAQPRYGVRKYSADLSAVGEPWLWGLGGAL
                     ALGIILVAGFLTMIFWNGVSTFWPKPIEVVTLTDGTVVAGEPTRSGVYRVGANVREGL
                     SPEALAMIDANDGFAHRVLYRSANFDLYGDDFRWVSDYEVAGVERPTDFFLLERQEWG
                     PFVGRIAGLTLDGRERRLEDYGYRELRRAQAQARQRFEAIRHIEQKDIGRVNYYIERE
                     RLALRRVQLRHGEDSEQYRAQQPQTAARIAELQQEFAAHQARITEIRTRDRGDWLLLE
                     EVGGRSKQQLLSEVVRLYPANDLSGLQKIGIYLSRWKEFLTAEPREANTEGGVLPAIF
                     GTVAMTLIMVVIVAPFGVITALYLREYAKQGRIVSIVRVSVNNLAGVPSIVFGVFGLG
                     FFAYTMGVSIDQLFYPERLPSPTFGTGGILWSALTLALLTVPVVIVAAEEALAAVPRS
                     MREGSLACGASKWQTIRRVVLPRAMPGIMTGLILAMARGAGEVAPLMLVGVVKLAPML
                     PVDGFYPFLHLERSFMHLGFHIFDVGFQSRNSEAGKPMVFVTTLLLLFLIVTMNSSAI
                     YIRNRLKRKFNVGQF"
     gene            complement(4762..7386)
                     /locus_tag="PLS34_06955"
     CDS             complement(4762..7386)
                     /locus_tag="PLS34_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP87241.1"
                     /translation="MESKTTSPAGGSDAHAFIVHRREKSTKKSVLLGDKLANWSITIG
                     GLLVIVAVITIMVFLISVAMPLAGRGELQEHRVYELATAKPVAWLNTDEYTTTAIRVA
                     ADGAVSAFHIPTGTFLSSAQLDFADRATAVAGIVTREQVAFGFADGTVRFGQFGFDIG
                     VVRPDQVPSGRQPLTERDSVAGATVYREMPTGDYRTVAPGWSVDAPQQVSDFAIVALD
                     YRVGGTVERPTRSFVTMDAAGVARISRAVVQRNLMTGAETVRLTSSTLPTLPEGTVVT
                     RVLMNSAADRVIVATDDGTLYRYDVRDFNNPVLAETTRAFDSDVAITAMDFLNAEHAM
                     VVGGSNGAVDVFFRLEQPDADTTDGYRLVRARSHENQGTPIVGISVAQRKKAMVTLGA
                     DGSAWVRYSTADRTMFRLQRQSDPAVDAALMLMPRMDGLLLTDRQGRVDSWSFYFPHP
                     ETTLKALFGKLWYEGYPEPTFTWQSSSGTDVFEPKYSLVPLIFGTLKATFYALLFAVP
                     ISLLGAIYTSEFLHRKVRASVKPVMEMMESLPTVVLGFVAALVLAPLVEEWIAAVLLA
                     FVSIPMGLMLGAYGWQMLPPAAALRLGGIPKFLFMTAAIFVFGFVAYLLGPSFERALF
                     YGDFKAWVNGDMGTGLPFLFLVGFPLSFMLVTFGFGRWYGHHWRLLLRERPRESAGRL
                     DFLRWVLFVAAAAAISWAVAALLTVAGYDPRGGFIDSYSQRNALVVGFVMGFAVIPNI
                     YTLAEDALNSVPSHLRAASLASGATPWQTAMWVILPVAASGVFSAVMIGMGRAVGETM
                     IVVMAAGNTPVLEWNIFSGLRTLSANIAVELPEAVKDSTLYRTLFLAALTLFVMTFVI
                     NTLAELVRQRFRKRAFQL"
     gene            complement(7479..>8237)
                     /locus_tag="PLS34_06960"
     CDS             complement(7479..>8237)
                     /locus_tag="PLS34_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRP87242.1"
                     /translation="IEGKGSGTAPPALIGGNAQFGPMSRPMKSAEIQEFEKRYGYPPT
                     AIRGAVDALAVYVHKDNPISCLSLQQVDAIFSKTRNGGHPQNITTWGDVGLTGEWASR
                     PISIYGRNSASGTYGYFKEVALFNGDYKDEVKEQPGSSTVVQGVASDIGGIGYSGVGY
                     KTADVRSVPLKTDADSGCFDALAEHAVSGDYPIARFLYIYVNKNPNEPLDPVRGEFIR
                     YVYSKQGQEAVVRDGYFPVLAPLAEQDLKAFGLK"
BASE COUNT         1380 a         2884 c         2854 g         1119 t
ORIGIN      
        1 agcgccaaga ggccgatcag cagccacgcg ccgccctgcg ccagccggcg catcgcgcgg
       61 ccgcgccggc gccggccggc ggcgaaatca gaagaccggc gaagggctga catcggattt
      121 tctcaacagg cggaacgccc aggcgttcag cggcagcacc agcgccgcca gcaacagtcc
      181 gagcacgaac ggcagctggg tgcgcacgtt ctccagcgcg tgcgtcccgg acatcagcgc
      241 gaccatctgc gcggtcagcg tggtgttggc ggccagcggg tcgagcgtga attgcgccac
      301 cagcccggcc gccatcagca ccaccacggt ctcgccgagc gcgcgcgaca ctgccagcag
      361 caccgcggcg ccgatgccgc ccgccgcact cggcagcacg acgtcgcgca gcacttccca
      421 gcgcgtggcg ccgaggccga gcgcagcgtc ggcggccgcc gtcggcaccg attcgagcgc
      481 gtcgccgatg cgcagtgcca gcaccggcag cagcatgatg cccaccacca ggccggccgc
      541 cagcaggttg ccgggcgcga ccgccagccc gacggcgccg gcgacggcgg cgaccgcggg
      601 cgctacggtc agcgctgcga acagcccgaa gatcaccgtg ggcacgaagg ccgcgacgcg
      661 gatgccgtgg cgccaggcgc ggcgaaagcg gcggcccgcc caggcgtggg catgcaacgc
      721 ggcgcccagc gccagcggca gggcgagcag cagggcgacg ccggccacca ccacgctgcc
      781 gagcagcacc ggcagcacgc cgaaggcgcc ctcgcccacc gactgcgccg cgcggatcgc
      841 gctttccggg ctccagtgcg taccggccag gaattcgcgc ggcgacacct gcaggaagaa
      901 attcactgcc gggacgacca gcgcggcgag cacgaccgcc agcagcgcgc ccgccacggc
      961 cgcgctcgac cacaaggtca agcccatgcc ccgctccagc gcccgctggg ccgggaccgc
     1021 ccgacgcagc gcactgctgc ccaccagcag cgccaccagc acgatcgcca gcaccacggc
     1081 cgtgacgcgt gccgacagcc cggcttcagc cgccaccggc ggtggcacag tgccgtgcgg
     1141 cggccgcgcc gccggcgcgg ccggcggcag cggcaccagg cccagccggg tcagccagcc
     1201 ctcgacgccc accgcatccg gcgcacgaaa cccggcgacg aacgcctcca gtcccggcac
     1261 gaagccccgg tgagcgggct tgaagtagag gtacagcgtg cgcgccagcg gatagtcgcc
     1321 ggcgctgatc gacgcggccg tcgggcaggc gccgtcgaag cacacgccgc gcacccgccc
     1381 ggcgttgcgc agcagcgcgc cgtagccgac cacgccgagc gccgccggat cgctgccgag
     1441 ccgcgccgcc agcacgtcgt cgttctcgcc ggcgtccacc caggcgccat cgcggcggat
     1501 ttcgccacag gcgcggcgct cggcatccgc gcgcggcgag tccgccagcg ccgcttcgca
     1561 ggccggtcgc aaggccagcg aagacaacgc gtcgcgcgtg cccgaggtgg ccggcggccc
     1621 gagcacgcga atcggcaggt cgggcaggtc ggcgcgaatc tcgctccagc gcgcatgggg
     1681 attgggcacc cagccggacc cgccgcgcgg gtcaggcacg cgccgcgcca ccgccagcca
     1741 cagctcggcc aggctgagcg cggcgatcgg atggccgggc gcgctcgcca gcacgatgcc
     1801 gtcgcgcccg accggcacgg cgtccagcgg cgtgacgccg gcgacgcggc aggcgctcgc
     1861 ctcggtgggc gtcatcggcc gcgaggccag caccacgtcg ggcgtggccg gccccagccc
     1921 ctcgcagaac caggcgatgc cggcgccggt gcccatcggc agcaccagcg gacccgggcg
     1981 cggcgcgcgc tcagcgaccg cggcggcaaa gggaaacacg gtcgaggaac cggccacgac
     2041 gacatggccg cgcggcgcgc cggccgcggc gagcgccgcc agcgcagcca gcagcgcggc
     2101 gagacccgcc agcagcgtgg cgaagaccgg ccggccgtta accgaaacgc cccgtgacat
     2161 agtcgctcgt gcgcttcgcc ttgggattgg tgaacaccgt gtcggtggcg ccgtactcga
     2221 tcagccggcc gagatacatg aaggccgtgt tgtcggagac gcgcgccgcc tgctgcatgt
     2281 tatgggtcac gatgacgatg gtgtagtggc ccttgagctc gtcgatcagc tcctcgatct
     2341 tggccgtcgc gatcgggtcg agcgccgagc acggctcgtc catcaacagg atctcggggt
     2401 cggaggcgat cgcgcgggcg atgcacagcc gctgctgctg gccgccggac agtccgagcg
     2461 ccgactcctg cagccggtcc ttgacttcgt tccacagcgc agcgcccttg agggctcgct
     2521 cgcaggtctc gttgagcacc ttcttgttgc tgacgccgtc cacgcgcagc gggtagacga
     2581 cgttttcgta gatcgacatg gcgaagggat tgggcttctg gaacaccatg cccatgcgct
     2641 tgcgcagggc gatcacgtcg atcgccggcc ggcagatgtt ttcgtcgttg acgatgatct
     2701 cgccggagat cttgaggtcg tccaccaggt cgttcatgcg gttgaggcaa cgcagcagcg
     2761 tcgacttgcc gcagcccgag ggcccgatca gggccgtcac cagtcccttc tggatttcca
     2821 tgttgacgtc gtacagcgcc tgcgcctcgc cgtaccagag attgaagttg cgcacgtcca
     2881 gcgccgcgcc ctccggcgag cgggtaacgt ggaacaccgt cggctcgata ccggcgactg
     2941 aggtgtccag gacttgcttt tcggcaacgg cattcatctt gaggctccgg ggtcgagatc
     3001 agaactggcc gacgttgaac ttgcgcttca gccggttgcg gatgtagatc gccgaggagt
     3061 tcatggtgac gatcaggaac agtagcagca aggtggtgac gaacaccatc ggcttgccgg
     3121 cctccgagtt gcgcgactgg aagccgacgt cgaagatgtg gaagccgagg tgcatgaagc
     3181 tgcgctcgag gtgcaggaag ggatagaagc cgtccaccgg cagcatcggc gccagcttca
     3241 ccacgccgac cagcatcagc ggcgccacct cgccggcgcc gcgcgccatg gcgaggatca
     3301 gcccggtcat gatgccgggc atggcgcgcg gcagcaccac gcgccggatg gtctgccact
     3361 tggaagcgcc gcaggccagc gagccctcgc gcatggagcg cggcaccgcg gcgagcgcct
     3421 cttcggcggc cacgatcacc accggcacgg tcagcaacgc gagggtcagc gccgaccaga
     3481 ggatgccgcc ggtgccgaag gtcggactgg gcaggcgctc cggatagaac agctggtcga
     3541 tgctcacgcc catggtgtag gcgaaaaagc cgaggccgaa cacgccgaac acgatcgacg
     3601 gcacgccggc caggttgttc accgacaccc gcacgatgga cacgatgcgg ccctgcttgg
     3661 cgtactcgcg caggtacagc gccgtgatca cgccgaaggg cgccacgatc accaccatga
     3721 tgagcgtcat ggccacggtg ccgaagatcg ccggcagcac cccgccctcg gtgttggcct
     3781 cgcgcggctc cgcggtcagg aactctttcc agcgcgacag gtagatgccg atcttctgca
     3841 atccgctgag gtcgttggcc ggatacagtc gcaccacctc ggagagcagc tgctgcttgc
     3901 tgcgcccgcc gacttcctcg agcagcagcc agtcaccccg gtcgcgtgtg cggatctcgg
     3961 tgatgcgcgc ctggtgcgcg gcgaactcct gctggagttc ggcgatgcgg gccgcggtct
     4021 gcggctgctg cgcacggtac tgctcggagt cctcgccgtg gcgcagctgc acgcgtcgca
     4081 gtgcgagccg ctcccgctcg atgtagtagt tgacccggcc gatgtccttc tgttcgatgt
     4141 ggcggatcgc ctcgaaccgt tggcgggcct gggcctgagc gcgcctgagc tcgcgatagc
     4201 cgtagtcctc cagccggcgt tcccggccgt ccagcgtcag gccggcgatc cggccgacga
     4261 acggccccca ttcctgtcgt tccagcagga agaagtccgt cggccgctcg accccggcca
     4321 cctcgtagtc cgacacccag cggaagtcgt cgccgtacag gtcgaaattg gcggagcggt
     4381 acagcacgcg gtgcgcgaag ccgtcgttgg cgtcgatcat ggccagcgcc tcgggcgaca
     4441 ggccttcccg gacgttcgcg ccgacacggt aaacgccaga acgcgtcggc tcgccggcca
     4501 ccaccgtgcc gtccgtcagc gtgacgacct cgatcggctt gggccagaag gtcgagacgc
     4561 cgttccagaa gatcatggtg aggaagcccg ccaccaggat gatgcccagc gcgagcgcgc
     4621 cgccgaggcc ccacaaccag ggctcgccga ccgccgagag gtcggcgctg tacttgcgca
     4681 cgccgtagcg cggctgggcg gcttcggtgg cggcgccggc ggcggtgtct gcggtgctgg
     4741 ccatgtgcgg gttctccgga atcacagctg gaaggcgcgc ttgcggaagc gctggcgcac
     4801 cagctcggcg agcgtgttga tgacgaaggt catgacgaac agcgtcagcg cggcgagaaa
     4861 cagcgtgcgg tacagcgtgc tgtccttgac cgcctcgggc agctcgacgg cgatgttggc
     4921 cgacagggtg cgcaggcccg agaatatgtt ccactcgagc accggcgtgt tgccggcggc
     4981 catcaccacg atcatggtct cgccgaccgc gcggcccatg ccgatcatga ccgccgagaa
     5041 caccccggac gccgccaccg gcaggatcac ccacatggcg gtctgccacg gggtggcccc
     5101 ggaggccagc gaggcggcgc gcaggtgcga gggcacggag ttgagcgcat cctcggccag
     5161 ggtgtagatg ttcgggatca ccgcgaagcc catgacgaag ccgaccacca gcgcgttgcg
     5221 ctgcgagtag ctgtcgatga agccgccgcg cggatcgtag ccggccacgg tcagcagcgc
     5281 ggcgacggcc cacgagatcg cggcggcggc ggcgacgaac agcacccagc ggaggaaatc
     5341 gagccggccg gccgactcgc gcggccgctc acgcagcagc agccgccagt ggtggccgta
     5401 ccagcggccg aagccgaagg tcaccagcat gaaggacaac gggaagccga ccaggaacag
     5461 gaacggcagg cccgtgccca tgtcgccgtt cacccaggcc ttgaagtcgc cgtagaacag
     5521 cgcccgctcg aaggacgggc cgagcaggta cgccacgaag ccgaacacga aaatcgcagc
     5581 ggtcatgaac aggaatttcg ggataccacc gaggcgcagc gccgccgccg gcggcagcat
     5641 ctgccagccg taggcgccga gcatcagccc catcgggatg gacacgaagg ccagcagcac
     5701 cgccgcgatc cactcctcca ccagcggtgc cagcaccagc gcggcgacga agccgagcac
     5761 cacggtgggc agcgactcca tcatttccat caccggcttc accgaggcgc gcaccttgcg
     5821 atgcaggaac tccgaggtgt agatggcgcc gagcagcgag atcggcaccg caaacagcag
     5881 cgcgtaaaag gtcgccttaa gggtgccgaa gatcagcggc accagcgagt acttgggctc
     5941 gaacacgtcc gtcccggatg acgactgcca ggtgaaggtc ggctccgggt agccctcgta
     6001 ccagagcttg ccgaacagcg ccttgagcgt ggtctcgggg tgggggaagt aaaaactcca
     6061 cgaatccacc cgcccctgcc ggtcggtcag cagcaggccg tccatgcgcg gcatcagcat
     6121 cagcgcggcg tccaccgccg ggtcgctctg ccgctgcagg cggaacatgg tgcgatccgc
     6181 ggtcgagtaa cgcacccagg cactgccgtc ggcgccgagc gtcaccatgg ctttcttgcg
     6241 ttgcgccacc gagatgccga cgatcggcgt gccctggttt tcgtggctgc gggcgcgcac
     6301 caggcgatag ccgtcggtgg tgtccgcatc cggctgctcg agccggaaga acacgtccac
     6361 cgcgccgttg gatccgccga ccaccatggc gtgctcggcg ttgagaaagt ccatcgccgt
     6421 gatcgccacg tcgctgtcga aggcccgggt ggtctcggcc agcaccgggt tgttgaaatc
     6481 gcgcacgtcg tagcggtaca gcgtgccgtc gtcggtggcg acgatgacgc gatccgccgc
     6541 gctgttcatc agcacccggg tcacgaccgt gccctccggc aaggtcggca gggtgctcga
     6601 ggtgagccgc acggtctcgg cgccggtcat gagattgcgc tggaccacgg cccgcgagat
     6661 gcgcgccacg ccggccgcgt ccatggtgac gaaggagcgg gtcggccgtt ccacggtgcc
     6721 gccgacgcgg tagtcgagcg ccacgatggc gaaatccgat acctgctgcg gggcatcgac
     6781 cgaccagccc ggcgccacgg tgcggtaatc gccggtgggc atttcgcgat agaccgtcgc
     6841 gccggcgacg ctgtcgcgct cggtcagcgg ctggcggccc gacggcacct ggtccggccg
     6901 caccacgccg atgtcgaagc cgaactgccc gaagcgcacc gtgccgtcgg cgaaaccaaa
     6961 ggccacctgc tcacgcgtca cgatgccggc cacggccgta gcacggtcgg caaaatccag
     7021 ctgggccgag gacaggaaag tgccggtggg aatgtggaag gcgctgaccg cgccgtcggc
     7081 cgcgacacgg atcgcggtgg tggtgtactc gtccgtgttc agccaggcga cgggcttggc
     7141 ggtggccagt tcgtagacgc ggtgctcctg cagctcgccg cggcccgcca gcggcatggc
     7201 cacggagatc aggaacacca tgatggtgat gacggcgacg atgaccagca ggccgccgat
     7261 ggtgatggac cagttggcga gcttgtcgcc cagcagcacg gatttcttgg tggacttttc
     7321 gcgccggtgc acgatgaagg catgcgcgtc gctgccgccc gccgggctcg tggtcttgct
     7381 ttccatctgc tctccacccc gcctggaggt gacttccaga aatcatgcag cggtgcggga
     7441 aaaccccgca ccgctgcagt ataaaacccc cgcagggttt acttcaggcc gaaagccttc
     7501 aggtcctgct ccgccagcgg cgccagcacc gggaagtagc cgtcgcggac gaccgcctcc
     7561 tggccctgct tggagtacac gtagcggatg aactcgccgc gcaccgggtc gagcggctcg
     7621 ttcgggttct tgttgacgta gatgtagagg aagcgcgcga tcgggtagtc gcccgacacc
     7681 gcgtgctcgg ccagcgcatc gaagcaaccg ctgtcggcgt ccgtcttcag cggcacggag
     7741 cgcacgtcgg cggtcttgta gccgacaccc gagtagccga tgccgccgat atcggaggcc
     7801 acgccctgca ccaccgtcga cgaccccggc tgttccttca cttcgtcctt gtagtcgccg
     7861 ttgaacagcg cgacttcctt gaagtaaccg taggtgccgg aggccgagtt gcggccgtag
     7921 atggaaatcg ggcggctggc ccactcgccg gtcaggccga cgtcacccca ggtggtgatg
     7981 ttctgcgggt gaccgccgtt gcgggtcttc gagaagatcg cgtcgacctg ctgcagcgac
     8041 aggcaggaga tcgggttgtc cttgtggacg tacacggcca gggcgtccac cgcgccgcga
     8101 atggcggtcg gcggatagcc gtagcgcttc tcgaactcct ggatctcggc cgacttcatc
     8161 ggccgggaca tggggccgaa ctgggcgttg ccgccgatca gcgccggcgg cgcggtgccc
     8221 gagcccttgc cctcgat
//
LOCUS       DAOMPG010000101         8148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3802_length_8148_cov_4.889163, whole genome shotgun sequence.
ACCESSION   DAOMPG010000101 DAOMPG010000000
VERSION     DAOMPG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3802_length_8148_cov_4.889163"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /locus_tag="PLS34_06965"
     CDS             <1..2517
                     /locus_tag="PLS34_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRP87243.1"
                     /translation="IGQSDTGIVDFVDHSVYVMTSEYGAASQLEKIDMLDFAEIVVLN
                     KFEKRGAEDALRDVRKQWKRNRKAFKATDDEIPVYPTIASQFNDPGVNRLFEALCARI
                     AVRCGLDPADWTPPSLPASTDHGALIPPRRVRYLAEIAEDGRRANDGAEQAAVAAGLA
                     WSCWRALEGLDPLRPAALEAYPEEAVAGNDALAPLRRRYNEALAGLGEEHVALLKAWP
                     ERRAAVEAAQYSYRVRDRELRGENYTESLSRLPLPRLAPPRYQDWGEILRFLVRENLP
                     GAYPYTGGVYPYRREEEDPIRMFAGEGMPERTNRRFHYLAAGHAATRLSTAFDSVTLY
                     GEDPDERPDVYGRIGNSGVSIATLDDMKRLYSGFDLCDPATSVSMTINGPAPMILAMF
                     MNTAVDQQVEKHLRATGGWEAAERRIGELLQGRERPVYRGELPAGHDGSGLGLLGVSG
                     EQLVDAETYGRIRAETLARVRGTVQADILKEDQAQNTCIFSTEFALRMMGDVQQYFIE
                     HGVRNFYSVSISGYHIAEAGANPVSQLAFTLANGFTIVEYYLARGMKIDDFAPNLSFF
                     FSNGMDPEYAVIGRVARRIWARAMKRLYGASARSQMLKYHIQTSGRSLHAQEMQFNDI
                     RTTLQAMYALFDNCNSLHTNAFDEALTTPTEASVRRAVAIQLIINRELGLNRNQNPWQ
                     GSFAIDYLTDQVEEAVYQEFERLAERGGVLGAMESMYQRGRIQEESLYYEHKKHDGSL
                     PLIGVNTFLGADGADEASVEEGQLIRSSEHEKQAQVSSTRAFNEAGGAAGLQALESLA
                     HAAASGGNVFGALMETVKRCSLGRISRALYEVGGQYRRNM"
     gene            2632..3411
                     /locus_tag="PLS34_06970"
     CDS             2632..3411
                     /locus_tag="PLS34_06970"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HRP87244.1"
                     /translation="MHQNRRWRPSAYAALALLAVLPLGPAAAQERTLNTTLSEQKSSV
                     QDAARSQARIAQLADQTTELLGEYRVVIQRLDRVRIYNENLQNLVRDQEREKQDIQRQ
                     LVSFQEVQQDIVPLMFEMIDDLAQLIELDMPFQLQERRGRVEMLRDIMDQSDVTVSEK
                     YRQIMNAYQIEADFGRTTEAYDGQLGDRKVDFLRVGRILLAYQTPDRAETGFWNKNTR
                     EWEVANDFRNDVVEGLRIARQQAAPNLLRLPVSAPETEQVQ"
     gene            3408..4751
                     /locus_tag="PLS34_06975"
     CDS             3408..4751
                     /locus_tag="PLS34_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP87245.1"
                     /translation="MRKLLLVAVALTVAFGAAPVQAVDSLDQLLQQVRKSRSELSAEN
                     RAREQRFQQNRNEQQALLRQAQQELAAEERRSDELTGQFNANELQLAELNDTLRTQLG
                     NFGELFGVVRQVSGDTVGLIRSSLVTAQDPGREELAERLAKVRGVPSMEDLNALRALL
                     TEEMVRGGKVARFDATITKPDGTSADETLVRIGVFNLISENGFLNYLPDSQTILELAR
                     QPQSRFVKMSNNLFNAAPNTTVAAAVDPGRGSLLALLIQEPSLGERIDQGGLVGYVII
                     ALGIIGLLIALVRWLYLGTVGTRIRGQLKSDEPKENNPLGRILKVLQDNQQADTETLE
                     LKLDEAILKEVPKLETWQGAIKVIAAVAPLLGLLGTVTGMIVTFQAITLFGTGDPKLM
                     AGGISQALVTTVLGLTVAIPLVLLHSVVASRSKALIEVLEEQSAGIIAKQAEAGR"
     gene            4748..5272
                     /locus_tag="PLS34_06980"
     CDS             4748..5272
                     /locus_tag="PLS34_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP87246.1"
                     /translation="MNPFVDAFVSIRDFFEAGGNVLYGILFVTVLMWTFIIERIWFFY
                     FLLPKRIEEVNQAWHARHDTTSWYASQVRAGLISEVRIESRRFLLLIKTLMAVLPLLG
                     LLGTVTGMIAVFDVMAFAGTGNARLMAAGVSKATIPTMSGLVAALSGLYFAAWLEKKA
                     TVEVDRVEGLLVHH"
     gene            5286..5693
                     /locus_tag="PLS34_06985"
     CDS             5286..5693
                     /locus_tag="PLS34_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP87247.1"
                     /translation="MRRRIARSEDEAEINITPMLDIVFIMLIFFIVTTSFVKEIGLEL
                     NRPSNEPQEEKKISEVIPIRIMANGMIEVSGRPVDIRAVRANVEGELATKGHSTPVVV
                     IADRSADSGLMVRVIDQARMAGAASVSIAAMAR"
     gene            5738..6148
                     /locus_tag="PLS34_06990"
     CDS             5738..6148
                     /locus_tag="PLS34_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP87248.1"
                     /translation="MARRHATREEETEINITPMLDIVFIMLIFFIVTTSFIRETGIDP
                     QRPEAVTADRQDRGNILIAISATGQIWMNKRQVEMDSVRQAVEQARNESPESTVVIIA
                     DERSETGILIDLMDQVRLGGILNISVAAQPSGGS"
     gene            6148..6774
                     /locus_tag="PLS34_06995"
     CDS             6148..6774
                     /locus_tag="PLS34_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRP87249.1"
                     /translation="MRFLLAIVAGTIMALVLFLLMHALIGGRDGFERPEDTGRVIDFV
                     RVRADEIVQTRERHVPRKPPPPDKPPPPPQLQTQAPQQVVRQTLDIETPDISVGMTGG
                     PVVAAAWQAGDVGQDGDIIPIVRIEPSYPRDALLRGLEGWVRVRITILPDGSVGNATV
                     VAAEPPRVFNREAVRAVLRWKFKPRIVDGVAVEREAEQTIDFNLDNPG"
     gene            6780..8069
                     /locus_tag="PLS34_07000"
     CDS             6780..8069
                     /locus_tag="PLS34_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87250.1"
                     /translation="MNNDAVRTSKVLAGLIAAVVLLGVSMPGAAQQEGPKPEIERKEP
                     GVLMGERAYRRFDTIQQLYSENKYQETLTAVQNYLATDLNPYERAMGEQIYGFVLISL
                     DRLDEAVPRFEKAIELNALPNNAHFNMMRSLAQLYASREEWQKSIDMMTQYLRYQPTP
                     SPEDSIMMAQNYAQMNRYRDALPWVDRAIAEGGAKAQESWYQLKLAIYFELKDYRGAL
                     PVLRILVSRWPNKLTYWETLAGAHQELNQDADALAALMTAYDGGLITTQPKLLNLVRM
                     SMYLDLPYQGGQILARAMEAGQVEANAANLRLLLQAWTSAREYNRAGAVIDRLAPLTG
                     EGDLFVQKARLMMEQNQWQATVDAARQAIELGNVDKPGAAWLMIGIAEMELDNLREAR
                     RAFQQAQEYDANTRRQAREWQRFVEDRIQVAELRSGQ"
BASE COUNT         1455 a         2680 c         2751 g         1262 t
ORIGIN      
        1 atcggccaga gcgacaccgg gatcgtcgac ttcgtcgacc actcggtgta cgtcatgacc
       61 agcgagtacg gcgccgcgag ccagctcgag aagatcgaca tgctggactt tgccgagatc
      121 gtggtgctca acaagttcga gaagcgcggc gccgaggatg cgctgcgcga cgtccgcaag
      181 cagtggaaac gcaatcggaa ggcgttcaag gcgaccgacg acgagattcc cgtctacccg
      241 accatcgcca gccagttcaa cgacccgggc gtgaaccggc tgttcgaggc cctgtgcgcc
      301 aggatcgcgg tgcgctgcgg gctggacccg gccgactgga cgccgccgtc cctgccggcc
      361 tcgaccgacc acggcgccct cattccgccg cgccgggtgc gttacctggc ggaaatcgcc
      421 gaggacggcc ggcgcgccaa cgacggggcg gagcaggccg cggtggccgc cggcctggca
      481 tggtcctgct ggcgggcgct ggaagggctt gatccgctgc ggcccgcagc gctcgaggcc
      541 tatccggagg aggcggtcgc cgggaacgat gcgctggcgc cgctgcggcg gcgctacaac
      601 gaggccctgg ccggcctcgg cgaggagcac gtggcgctcc tgaaggcctg gccggagcgg
      661 cgtgcggcgg tggaggcggc gcagtacagc taccgggtgc gcgaccgcga actgcgcggc
      721 gagaactaca ccgaatcgct gagccgcctg ccgttgccgc ggcttgcgcc gcccaggtac
      781 caggactggg gcgagatcct gcggttcctg gtccgggaga acctgcccgg cgcctacccg
      841 tataccggcg gcgtctatcc ctatcgccgc gaggaagagg acccgatccg catgttcgcg
      901 ggcgagggca tgccggagcg caccaatcgg cgcttccatt atcttgccgc cggccatgcg
      961 gccacccgcc tgtcgacggc cttcgattcc gtgaccctgt acggcgagga cccggacgag
     1021 cggcccgacg tctacgggcg catcggcaat tccggggtgt cgattgcgac cctcgacgac
     1081 atgaagcgcc tgtactcggg cttcgatctg tgcgacccgg cgacctcggt ctcgatgacc
     1141 atcaacggcc cggcgccgat gatcctggcg atgttcatga acacggccgt cgaccagcag
     1201 gtcgagaagc acctgcgggc caccggcggc tgggaggctg cggagcgccg catcggcgaa
     1261 ttgctgcagg gccgcgagcg tcccgtgtac cgcggcgagt tgccggccgg acacgacggc
     1321 tcgggactcg gcctgctcgg cgtctcgggc gagcagctgg tggatgcgga gacctatggg
     1381 cggattcgtg ccgagaccct ggcgcgggtc cgcggcacgg tgcaggccga catcctcaag
     1441 gaggaccagg cccagaacac ctgcattttc tcgaccgagt tcgcgctgcg catgatgggg
     1501 gacgtacagc aatacttcat cgagcacggc gtgcgcaatt tctattccgt gtcgatcagc
     1561 ggctaccaca tcgccgaggc cggggcgaac ccggtcagcc agctggcgtt cacgctggcg
     1621 aacggcttca ccatcgtcga gtactacctg gcccgcggca tgaagatcga cgattttgcg
     1681 ccgaacctct cgtttttctt ctcgaacggc atggatcccg agtatgcggt catcggccgg
     1741 gtggcgcgcc gcatctgggc gcgggccatg aagcggctct acggcgcctc ggcccgcagc
     1801 cagatgctca agtaccacat ccagacctcg ggccgcagcc tgcatgccca ggagatgcag
     1861 ttcaatgaca tccgcaccac gctgcaggcc atgtacgcgc tgttcgacaa ctgcaacagc
     1921 ctgcacacca atgctttcga cgaggccctc accacgccca ccgaggcctc ggtgcgccgt
     1981 gcggtcgcca tccagctgat catcaaccgc gagctcggcc tgaaccgcaa ccagaacccc
     2041 tggcagggat cgttcgcgat cgattacctg accgaccagg tcgaggaggc cgtctaccag
     2101 gagttcgagc ggctcgccga gcgcggcggg gtgctgggcg ccatggagtc catgtaccag
     2161 cgcggccgga tccaggaaga aagtctttat tacgaacaca agaagcacga cggcagcctg
     2221 ccgctgatcg gcgtcaacac cttcctcggg gcggacggcg cggacgaggc ctcggtcgag
     2281 gagggccagc tgattcgctc cagcgagcac gagaagcagg cccaggtgtc gtcgacgcgg
     2341 gccttcaacg aggccggcgg cgcggccggc ttgcaggcgc tggagtcgct ggcgcacgcc
     2401 gcggccagtg gcggcaacgt gttcggggcg ctcatggaga cggtcaagcg ctgctcgctg
     2461 ggccgcatca gccgggctct ttacgaggtc ggcggccagt accggcgcaa catgtagccg
     2521 ggcccctgcg tgcttttcat ccggggacga ttctgctagc ttctcacgct tcacgcggca
     2581 ccgcccgcgg ctgcacctca tacctgcatt tgcccaattg aagaggttct catgcatcaa
     2641 aacaggagat ggcgcccgag tgcgtacgcc gccctggcgc tgctggcggt tctgccgctc
     2701 ggcccggcgg ccgcgcagga acgcactctg aacaccacgc tgtcggagca gaaatccagc
     2761 gtccaggatg cggcgcggtc ccaggcgaga atcgcccagc tggctgacca gacgaccgaa
     2821 ctgctcggcg agtaccgggt ggtgatccag cggctcgacc gggtgcggat ctacaacgag
     2881 aacctgcaga acctggtgcg cgaccaggag cgcgagaagc aggatatcca gcggcagctg
     2941 gtcagcttcc aggaagtcca gcaggacatc gtcccgctga tgttcgagat gattgacgac
     3001 ctggcgcagc tgatcgagct ggatatgccg ttccagttgc aggaacgtcg cggccgggtc
     3061 gagatgctgc gcgacatcat ggaccagtcc gacgtgaccg tgtcggagaa gtaccggcag
     3121 atcatgaacg cctaccagat cgaggcggac ttcggtcgca ccaccgaggc ctacgacggc
     3181 cagctgggcg accgcaaggt ggacttcctg agggtcggcc ggatcctgct ggcctaccag
     3241 acgccggatc gcgctgagac cggcttctgg aacaagaaca cccgcgagtg ggaggtcgcc
     3301 aacgacttcc gcaacgacgt cgtcgaaggg ttgcggattg cgcgccagca ggcagcgccg
     3361 aacctcctgc ggctccccgt gtccgccccc gagacggagc aggtgcaatg agaaagcttc
     3421 ttttggtcgc ggtcgcgctg acggtcgcat tcggggccgc gccggtccag gcggtcgatt
     3481 cgctcgacca gctcctgcag caggtccgca agtcgcgcag cgagctcagc gccgagaaca
     3541 gggcgcgcga gcagcgattc cagcagaatc gcaacgagca gcaggcgctg ctgcgccagg
     3601 cgcagcagga gctggcggcc gaggaacgcc gcagcgacga gttgaccggg cagttcaacg
     3661 ccaacgagtt gcagctggct gagttgaacg acaccttgcg cacgcagctc ggcaacttcg
     3721 gcgagctgtt cggcgtggtg cggcaggtgt ccggtgatac ggtcggcctg atccgcagct
     3781 cgctggtcac ggcccaggat ccgggccggg aggagctcgc ggaacggctg gccaaggtgc
     3841 gcggcgtacc ctccatggag gatctcaatg ccctgcgcgc actgctcacc gaggagatgg
     3901 tgcgcggcgg caaggtggcg cgcttcgacg ctaccatcac caagccggac ggcacctcgg
     3961 cggacgagac gctggtgcgc atcggcgtgt tcaacctgat ctccgagaac ggcttcctca
     4021 attacctgcc ggattcgcag accatcctgg aactggcgcg gcagccgcag tcacgcttcg
     4081 tcaagatgtc gaacaacctg ttcaacgcgg cgccgaacac cacggtggcg gcggccgtcg
     4141 atccgggccg cggctcgctg ttggcactgc tcatccagga gccgagcctc ggcgagcgca
     4201 tcgaccaggg cggcctggtg ggctacgtca tcattgccct cggcatcatc ggcctgctga
     4261 tcgcgctggt ccgctggctc tacctgggca ccgtcggtac ccggatccgc ggccagctca
     4321 agtccgacga gcccaaggaa aacaacccgc tgggccggat cctgaaggtc ctccaggaca
     4381 accagcaggc ggacaccgag acgctcgagc tcaagctcga cgaggcgatc ctcaaggaag
     4441 tgcccaagct cgagacctgg cagggtgcca tcaaggtgat cgctgccgtc gcgccgctgc
     4501 tcggcctgct cggcacggtg accggcatga tcgtgacctt ccaggcgatc accctgttcg
     4561 gcaccggcga tcccaagctg atggccggcg gcatctcgca ggcgctggtc acgaccgtgc
     4621 tcggcctgac ggtggccatc ccgctggtgc tgctgcacag tgtggtcgcc agccgcagca
     4681 aggccctcat cgaggtgctg gaagaacaga gcgcgggcat catcgccaag caggcggaag
     4741 ccggcagatg aacccgttcg tagacgcgtt cgtatcgatc cgcgatttct tcgaagcggg
     4801 cgggaacgtc ctctacggca tcctcttcgt gacggtgctg atgtggacct tcatcatcga
     4861 gcgcatctgg ttcttttact tcttgctgcc gaagcggatc gaggaggtca accaggcctg
     4921 gcacgcccgc cacgacacga cttcctggta cgccagccag gtccgggccg ggctgatctc
     4981 ggaggtgcgc atcgagagcc ggcgcttcct gctgctgatc aagacgctga tggcggtgct
     5041 gccgctgctc ggcctgctcg gtaccgtcac gggcatgatc gcggtgttcg acgtcatggc
     5101 cttcgccggg acgggcaacg cgcggctgat ggcggccggc gtgtccaagg cgaccatccc
     5161 gaccatgtcc ggcctcgtcg cggccctgtc gggcctgtat ttcgccgcct ggctggaaaa
     5221 gaaggccacc gtcgaagtcg accgggtcga aggcctgctg gtgcaccatt gaggactgag
     5281 gcggcatgag acgcaggatc gcaagaagcg aggacgaagc cgagatcaac atcacgccga
     5341 tgttggacat cgtcttcatc atgctgattt tcttcatcgt gacgacctcc ttcgtgaagg
     5401 agatcggcct cgagctgaac cggccgtcga acgagccgca ggaagaaaag aagatttccg
     5461 aggtcattcc gattcgcatc atggccaatg gcatgatcga ggtgagcggc cggccggtgg
     5521 acatccgcgc ggtgcgggcc aacgtcgagg gcgagctggc caccaagggg cacagcacgc
     5581 cggtggtggt gatcgccgac cgctccgccg attcggggct gatggtgcgg gtcatcgacc
     5641 aggcgcggat ggccggggcg gcctcggtgt cgattgcggc catggcccgc tgacgccggc
     5701 ggcgaaactt taggcacgga ccgggactct caactacatg gcaagaaggc acgcgacgcg
     5761 cgaggaagag accgagatca acatcacgcc gatgttggat atcgtcttca tcatgctgat
     5821 tttcttcatc gtgacgacct ccttcatccg cgagaccggc atcgatccgc agcgcccgga
     5881 ggccgtcacc gcggaccgcc aggaccgcgg caacatcctc atcgccatct ccgcgaccgg
     5941 ccagatctgg atgaacaagc gccaggtgga gatggattcc gtgcggcagg cggtcgagca
     6001 ggcgcgcaac gaaagcccgg agagcaccgt ggtgatcatc gccgacgagc gctcggagac
     6061 cggcatcctc atcgacctga tggaccaggt ccgcctgggc ggtatcctga acatctcggt
     6121 ggcggcgcag ccgtcggggg gtagctgatg cgattcctgc tcgccatagt ggccggcacg
     6181 atcatggccc tggtgctgtt cctgttgatg cacgcgctca tcggcggccg ggacggcttc
     6241 gaacggccgg aggataccgg ccgggtgatc gacttcgtgc gggtgcgcgc ggacgagatc
     6301 gtgcagacgc gcgagcgcca cgtgccgcgc aagccgccgc cgccggacaa gccgccgccg
     6361 ccgccgcagt tgcagaccca ggcgccgcag caggtggtca ggcagaccct cgacatcgag
     6421 accccggaca tttccgtcgg catgaccggc ggaccggtgg tggccgccgc ctggcaggcc
     6481 ggcgacgtcg gccaggacgg cgacatcatt cccatcgtgc gcatcgagcc gagctatccc
     6541 cgtgacgcgt tgctgcgcgg gctggaaggc tgggtgcggg tgcgcatcac catccttccc
     6601 gacgggtcgg tgggtaatgc gacggtggtc gccgccgagc cgccgcgggt gttcaaccgc
     6661 gaagccgtgc gggcggtgct gcgctggaag ttcaagccgc ggatcgtaga cggcgtcgcg
     6721 gtcgagcgcg aggccgagca gaccatcgat ttcaacctgg acaaccccgg ctagcgatga
     6781 tgaacaacga cgccgtacgc acgagcaaag tcctcgccgg cctgattgcg gccgtggttc
     6841 tcctgggcgt gtccatgccc ggcgctgcgc agcaggaggg acccaagccc gaaatcgagc
     6901 gcaaggagcc cggcgtgctg atgggcgagc gcgcctaccg gcgcttcgac accatccagc
     6961 agctgtacag cgagaacaag taccaggaga ctctcacggc ggtccagaat tacctggcga
     7021 ccgacctgaa tccctacgag cgtgccatgg gcgagcagat ctacggcttc gtgctgatct
     7081 cgctcgaccg gctcgacgag gccgtgccgc gcttcgagaa ggcgatcgag ctgaatgcgt
     7141 tgcccaacaa cgcccacttc aacatgatgc gttcgctggc gcagctctat gcgtcccgtg
     7201 aagagtggca gaaatccatt gacatgatga cccagtacct ccgctaccag ccgacgccgt
     7261 cgccggagga cagcatcatg atggcgcaga actacgcgca gatgaatcgc taccgggacg
     7321 ccctgccgtg ggtcgaccgg gcgatcgcgg aaggcggcgc caaggcccag gagagctggt
     7381 accagctcaa gctggcaatc tactttgagc tcaaggacta tcgcggcgcg ctcccggtgt
     7441 tgcgcatcct cgtatcccgc tggccgaaca agctgaccta ctgggagacc ctggcgggcg
     7501 cccaccagga actcaaccag gacgccgacg cgctcgctgc gctgatgacc gcctacgacg
     7561 gcggcctcat caccacccag cccaagctgc tcaacctggt gcgcatgagc atgtacctgg
     7621 acctgcccta ccagggcggg cagattcttg ccagggcgat ggaggccggc caggtggagg
     7681 cgaacgccgc caacctgcgc ctgctgctgc aggcctggac cagtgcacga gaatacaatc
     7741 gggccggcgc cgtcatcgac cggctggcgc cgctgacggg cgagggcgac ctgttcgtgc
     7801 agaaggcgcg cctgatgatg gagcagaacc agtggcaggc gaccgtggac gccgcccgcc
     7861 aggccatcga gctcggcaac gtcgacaagc cgggcgccgc gtggctgatg atcggcatcg
     7921 ccgagatgga gctggacaac ctgcgcgagg cgcgccgcgc cttccagcag gcccaggaat
     7981 acgacgccaa cacgcgccgc caggcgcgcg aatggcagcg cttcgtcgag gaccgcatcc
     8041 aggtcgccga gctgcgcagc ggccagtaat cgaacggccc attgccttgc cgggcggtgc
     8101 cgtcgcccgg cgcgccgtat tatgtgaccc gtttcacctt atgttaat
//
LOCUS       DAOMPG010000102         8091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3838_length_8091_cov_3.979592, whole genome shotgun sequence.
ACCESSION   DAOMPG010000102 DAOMPG010000000
VERSION     DAOMPG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_8091_cov_3.979592"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLS34_07005"
     CDS             complement(<1..465)
                     /locus_tag="PLS34_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRP87251.1"
                     /translation="MSGPVPVAILGASGRMGRTLLRCLPEFPSLGLAGALTAPGAGLV
                     GRDVGEVAGLGPLGVALSEDVAAAIRTARVAVDFTLAEAVPATAAACRAAGVALVCGV
                     TGLDARAQAALDDAATTVPVLWSPNMSAGVAVLERLTALAAAALADFDAGIVD"
     gene            complement(462..1601)
                     /gene="dnaJ"
                     /locus_tag="PLS34_07010"
     CDS             complement(462..1601)
                     /gene="dnaJ"
                     /locus_tag="PLS34_07010"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRP87252.1"
                     /translation="MAKRDYYEALGLQRNATEAELKQAYRRLAMKFHPDRNPGDPEAE
                     AAFKEVKEAWEVLKDPRKRAAYDQFGHAGVGGGGPGGPGGFGPGEAFSDIFGEVFGDI
                     FGVGRRSRSRVFRGADLRYELELELEQAAFGDTVTITLPLAAECGECHGSGARAGASP
                     VNCRTCGGRGSVRMQQGFFAVEQTCPECRGQGTVIEDPCDACHGEGRVPHNKTLAVKI
                     PAGVDSGDRIRLGGEGEAGRNGGPPGDLYVEVVVRPHPIFERRGADLACEIPVGFVAA
                     ALGAEVEVPTLGDASAEQLKVPEGTQSGKVFRLRGKGIRPVRGGPQGDLLVHVVVETP
                     VGLKKRQKELLREFEASLRDSGRSHSPREQGWMDKVRGFFDRLGA"
     gene            complement(1690..>2698)
                     /locus_tag="PLS34_07015"
     CDS             complement(1690..>2698)
                     /locus_tag="PLS34_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRP87253.1"
                     /translation="ESLVEELVRRTIEPCKIAIKDAGLKVGDIQDVILVGGQTRMPKV
                     QEAVKDFFGKEPKKDVNPDEAVAIGAAVQAGVLSGEVKDVLLLDVTPLSLGIETLGGV
                     MTKLIEKNTTIPTKAHQVFSTADDNQTAVTIHVLQGERERALDNKSLGRFDLTDLPLA
                     PRGVPQIEVTFDIDANGILNVSAKDKATGKEQKIVIKASSGLTDEEIKQMVADAEAHA
                     AEDKKFRELVDARNQAEALIHSSEKSLQDLGEKVQAEERARIESAVSDLKDAVKSDDK
                     GRIDAKSRALAEASASVMQRVYAEEAKAGTADGGEQSSANKPGEEDVVDAEFEEVKED
                     K"
     assembly_gap    2699..2708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2709..3594)
                     /locus_tag="PLS34_07020"
     CDS             complement(<2709..3594)
                     /locus_tag="PLS34_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRP87254.1"
                     /translation="MGKVIGIDLGTTNSCVAIMEGKNPKVIENSEGDRTTPSTVAFTK
                     DGEVLVGQSAKRQAVTNPQNTLFAVKRLIGRRFDDPVVAKDRDMVPYKIIQADNGDAW
                     VEAGGKKMAPPEISARVLMKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGKI
                     AGLEVKRIINEPTAAALAYGLDKQRGDRKVAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLKIIQYLAGSFEKESGIDIRKDALAMQRLKEAAEKAKIELSSS
                     QQTEVNLPYITADASGPKH"
     gene            complement(3692..4231)
                     /gene="grpE"
                     /locus_tag="PLS34_07025"
     CDS             complement(3692..4231)
                     /gene="grpE"
                     /locus_tag="PLS34_07025"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRP87255.1"
                     /translation="MSEMDKDAANEQVTAPGEVGDPNLEPEARLRRAEETATEFREQF
                     LRTAAELENVRRRAARDVESAHRFGVERFARELLAVVDSLEMGLEAARGAGDHNAVVE
                     GMEATLRLLVSVLEKFGVTPVEAAGAPFDPEAHEAIMTQPSEEAAPDTVIGVIQAGYR
                     IHDRLLRPARVIVARAPDE"
     gene            complement(4328..5386)
                     /gene="hrcA"
                     /locus_tag="PLS34_07030"
     CDS             complement(4328..5386)
                     /gene="hrcA"
                     /locus_tag="PLS34_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRP87256.1"
                     /translation="MANKPVEAELSERARHLLKVLVERYIRDGQPVGSRTLSKDSGLD
                     LSPATIRNVMADLEEMGLVVAPHTSAGRVPTPKGIRLFVDALLKVQPLSDAAIRRLQA
                     EMDAVRSGDGDVVGSASALLSSLTRMAGVVTVPRRTQASLRQIEFLPLSDHRILAILV
                     IDEKEVQNRILQMDRDYAGEELRQAANYLNEQFAGQSLASVREKLLAELADTRETMNA
                     MMQGAISMARQTFETVDDPGQGFVLAGETNLMQFAELSNIERLRGLFEAFARQRDILH
                     LLDQCLAAESLQIFIGEESGYRILESVSVVASPYSIDDEVVGVLGVIGPTRMAYDRII
                     PIVDITARLLGSALNSRD"
     gene            5511..6419
                     /locus_tag="PLS34_07035"
     CDS             5511..6419
                     /locus_tag="PLS34_07035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HRP87257.1"
                     /translation="MKRSFHTIALTGKYRDPRVGDSMLILARHLAAAGVRVLLDPEIR
                     MDFPGTAVESLPEGELADAADLVVAVGGDGTMLYASRLVAGRDVPLLGINRGRLGFLA
                     DITPGEMLRRLDEVLAGDYDEERRLMLEAVIDRGDGAPRRALALNDVVLQKRDTGRML
                     DFENWVNGVYVNTHGGDGLVIATPTGSTAYALSGGGPIIHPSLDAITLVPICPHTLSD
                     RPIVIRGDARIEVRVLERPDTRAEVACDGQPLGELAAGERLLVQAAAERVLLIHPRGH
                     DYFRLLRSKLHWGRGSQSRFDGTSEA"
     gene            6429..>8091
                     /gene="recN"
                     /locus_tag="PLS34_07040"
     CDS             6429..>8091
                     /gene="recN"
                     /locus_tag="PLS34_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRP87258.1"
                     /translation="MLSTVSVRNFAIIDEVSLELGPGLTVLTGETGAGKSILVDALGL
                     ALGDRADASAVRHGAERAEITASFDLATLPEVAAWLTEQALDADGECVLRRVVGAEGR
                     SRAWINGAQTTLQTLRELGERLVDIHGQHEHQSLTRPGVQRELLDARLPEPAPARAVA
                     EAWQAWRVALDERRRLEGAGRDREQRLDLLRYQHRELEAFAPRAGEAAELAAEFLQLA
                     NAGKLAEGAGGALERVYEGESGAAHDAVAQAAEALAELAELDPRLAAPQRLLAEAEIL
                     LAEAATELRRYLGNLDMDPARLDAVQSRLEDMKSLAHKHQVVAEALPERLAALAAEIE
                     ELEQAEVRLGRSAEKLAAAEAEYRKAAAKLGKARAAAAREFSAQVSALMHQLGMPGGR
                     FVVAVEHDPQAECASHGLDSIEFLVSANPGQPPAPVSKVASGGELSRISLAIQVAAKS
                     DHPIPVMVFDEVDSGVGGGVAEIVGRRLAEIGERAQVLCVTHLPQVASQARHHLRVVK
                     LTDGEVTRTTLKALTEAEKVEELARMLGGVEITSTTRAHAREMRKR"
BASE COUNT         1135 a         2901 c         2758 g         1287 t
ORIGIN      
        1 gtcgacgatg ccggcgtcga agtcggccag cgccgccgcg gccagcgctg tgagccgttc
       61 cagcaccgcc acgccggcgc tcatgttggg cgaccagagc accggcaccg tcgtggcggc
      121 atcgtccagc gccgcctggg cgcgcgcatc gagcccggtc acgccgcaca ccagcgccac
      181 gccggccgcg cggcaggccg cagccgtggc gggcaccgct tccgcgagcg taaagtccac
      241 cgcgacgcgc gccgtgcgga tcgccgcggc cacgtcctcg ctgagcgcca cgccgagcgg
      301 gccgagcccg gctacctcgc cgacgtcgcg cccgacgaga ccggcgccgg gcgcggtcag
      361 cgcgccggcc aggccgagcg acgggaattc gggcaggcag cgcaacagcg tccggcccat
      421 gcggccggag gcgccgagga tcgcgaccgg gacggggccg ctcatgcgcc gagcctgtcg
      481 aaaaagccgc gcaccttgtc catccagccc tgctcgcgcg gactgtggct gcggccgctg
      541 tcgcgcaggc tggcctcgaa ctcccgcaag agttccttct ggcgcttctt caggcccacc
      601 ggcgtctcca ccacgacatg caccagcagg tcgccctgcg ggccgccccg caccgggcgg
      661 atgcccttgc cgcgcaggcg gaacaccttg ccactctgcg tgccctcggg caccttgagc
      721 tgctctgcgc tcgcgtcgcc gagggtcggc acctccacct cggcgcccag cgccgcggcg
      781 acgaagccga ccgggatctc gcaggccagg tcggcgccgc gccgctcgaa gatcgggtgc
      841 gggcgcacca ccacctcgac gtacaggtca ccgggcgggc caccgttgcg tcccgcctcg
      901 ccctcgccgc ccaggcggat gcgatcaccg ctgtcgacgc cggccgggat cttcacggcc
      961 agcgtcttgt tgtgcggtac gcggccctca ccgtggcagg catcgcaggg gtcttcgatc
     1021 accgtgccct ggccgcgaca ctcgggacag gtctgctcga ccgcgaagaa gccctgctgc
     1081 atgcgcaccg agccgcgccc gccgcaggtc cggcagttga cgggcgacgc acccgcgcgg
     1141 gcgccgctgc cgtggcattc gccgcactcc gcggccagcg gcagggtgat ggtcacggta
     1201 tcgccgaagg cggcctgttc gagctcgagc tcgagctcgt aacgcaggtc ggcgccgcgg
     1261 aacacccgcg agcgactgcg gcgtccgacg ccgaaaatgt cgccgaacac ctcgccgaag
     1321 atgtcgctga aggcttcgcc cgggccgaag ccgccggggc cgcccggccc gccgccgccc
     1381 acgccggcat ggccgaactg gtcgtaggcg gcgcgcttgc gggggtcctt gagcacctcc
     1441 caggcctcct tgacctcctt gaaggccgcc tcggcctccg ggtcgccggg attgcgatcc
     1501 ggatgaaatt tcatggcgag gcgccgatag gcctgcttca gctccgcttc agtggcgttg
     1561 cgctgcagcc cgagggcctc gtagtaatcc cgcttcgcca tccggcgctc ccgaattggg
     1621 gacattcccc tttttgccag gcaaaaaggg gaatgtcccc aatagcccga aaatggggtc
     1681 tgaccccggt tacttgtctt ccttgacttc ctcgaactcg gcgtcgacga catcctcctc
     1741 gcccggcttg ttggcggatg actgctcgcc gccatcggcc gtgccggcct tcgcctcttc
     1801 ggcgtagacc cgctgcatca cgctcgccga ggcctcggcg agcgcgcgcg acttggcgtc
     1861 gatccgcccc ttgtcgtcgg acttcacggc gtccttgagg tcggagaccg ccgactcgat
     1921 tctggcgcgc tcctcggcct ggaccttctc gccgagatcc tgcagcgact tctcgctgga
     1981 gtggatcagc gcctcggcct ggttgcgcgc gtccaccagc tcgcggaact tcttgtcctc
     2041 ggcggcatgc gcctcggcgt ccgccaccat ctgcttgatc tcttcgtcgg tcaggccgct
     2101 cgaggccttg atgacgatct tctgctcctt gccggtggcc ttgtccttgg ccgacacgtt
     2161 gaggatgccg ttggcgtcga tgtcgaaggt gacctcgatc tgcggcacgc cgcgcggcgc
     2221 cagcggcagg tcggtcaagt cgaaacggcc gagtgacttg ttgtccagcg cccgctcgcg
     2281 ctcaccctgc agcacgtgga tggtgaccgc cgtctggttg tcgtccgcgg tggagaatac
     2341 ctggtgcgcc ttggtcggaa tggtggtgtt cttctcgatc agcttggtca tcacgccgcc
     2401 gagcgtctcg atgccgagcg acagcggggt gacatccagc agcagcacgt ccttgacctc
     2461 gcccgacagc acgccggcct gcaccgccgc accgatggcc acggcctcgt ccgggttgac
     2521 gtccttcttc ggttccttgc cgaagaaatc cttcaccgcc tcctgcacct tcggcatgcg
     2581 ggtctggcca ccgaccagga tcacgtcctg gatgtcgccg accttaaggc cggcgtcctt
     2641 gatggcgatc ttgcagggct cgatggtccg ccgcaccagt tcctcgacca gcgactccnn
     2701 nnnnnnnngg tgcttcggcc cgctggcgtc ggcggtgatg tacggcaggt tgacctcagt
     2761 ctgttgcgag gaggacagct cgatcttggc cttctcagcc gcctccttga ggcgctgcat
     2821 ggcgagcgca tccttgcgga tgtcgatgcc gctctccttc tcgaagctgc ccgccaggta
     2881 ctggatgatc ttaagatcga agtcctcacc gccgaggaag gtgtcgccgt tggtcgccag
     2941 cacttcgaac tggtgctcgc cgtccacctc ggcaatctcg atgatcgaga tgtcgaaggt
     3001 gccgccgccg aggtcgtaga ccgccacctt gcggtcgccg cgctgcttgt ccagcccgta
     3061 ggccagcgct gccgcagtcg gctcgttgat gatgcgcttg acctcgaggc cggcaatctt
     3121 gccggcgtcc ttggtggcct ggcgctggga gtcgttgaaa taggccggca ccgtgatcac
     3181 ggcctcggtg acgggctcgc cgagatagtc ctcggcggtc ttcttcatct tcatgagcac
     3241 gcgcgccgag atctcgggcg gcgccatctt cttgccgccg gcctcgaccc aggcgtcgcc
     3301 gttgtccgcc tggatgatct tgtagggcac catgtcgcgg tccttggcca cgaccgggtc
     3361 gtcgaaacgg cggccgatca gccgcttgac cgcgaacagg gtgttctggg gattggtcac
     3421 ggcctggcgc ttggccgact ggcccaccag cacctcaccg tccttggtaa aggcgacggt
     3481 cgacggggtg gtccgatcgc cttcgctgtt ctcgatgacc ttcgggttct tgccctccat
     3541 gatggccacg caggagtttg tggtccccag gtcgattccg atcaccttgc ccatgttcat
     3601 cgtctccagt ttgaattctt gcttcggcct cgcggcccgc tgcccctcaa gatgggggcc
     3661 cgatcagtgc tttcaagccc ctcggggcgg ctcattcgtc gggtgcgcgc gccacgatca
     3721 cccgcgcggg gcgcagcagc cgatcgtgga tccggtaacc cgcctggatc acgccgatca
     3781 ccgtgtccgg ggcggcttcc tcggagggct gggtcatgat ggcctcgtgc gcctcgggat
     3841 cgaagggtgc gccggcggcc tccaccgggg tcacgccaaa cttctccagc accgacacca
     3901 gcagccgcag ggtcgcctcc atgccctcga cgacggcgtt gtgatcgccc gccccgcgag
     3961 ccgcctccag ccccatttcc aggctgtcca ccaccgccag cagctcgcgc gcgaagcgct
     4021 ccacgccgaa gcggtgcgca ctctcgacgt cgcgcgccgc gcgccgccgc acgttctcca
     4081 gctccgccgc ggtgcgcagg aactgctcgc ggaactccgt cgcggtctcc tcggcgcgtc
     4141 gcagcctggc ctccggctcg aggttcgggt cgccgacctc accgggcgcc gtaacctgct
     4201 cattcgcggc gtccttgtcc atctcgctca ttggccgtgt ctccgtgcgc atgaccgcgg
     4261 ccggccgaaa tcggccggtc ccgccatgca aagcgctgaa ttatgcccgt ttctcgggac
     4321 ttggggccta gtcgcgggaa ttcaaggccg agccgagcag gcgcgccgtg atgtccacga
     4381 tggggatgat ccggtcgtag gccatgcggg tcgggccgat cacgccgagc acgccgacca
     4441 cctcgtcgtc gatggagtac gggctggcca cgacgctgac cgattccagg atacggtagc
     4501 ccgactcctc gccgatgaag atctgcaggc tctcggcggc caggcactgg tcgagcaggt
     4561 gcaggatgtc tctttggcgg gcaaaggcct cgaacagccc gcgcagccgc tcgatgttgg
     4621 acagctcggc gaactgcatc aggttggtct cgccggcgag cacgaagccc tggccgggat
     4681 cgtcgaccgt ctcgaaggtc tgccgggcca tgctgatggc gccctgcatc atggcgttca
     4741 tggtttcgcg ggtgtccgcc agctcggcca gcagcttctc gcgcacgctc gccagcgact
     4801 ggcccgcgaa ctgctcgttc aggtagttgg ccgcctggcg cagttcctcg ccggcgtagt
     4861 cgcggtccat ctgcaggatg cggttctgca cctctttctc gtcgatcacc aggatcgcca
     4921 ggatgcggtg gtccgacagc ggcaggaact cgatctggcg cagcgaggcc tgggtgcgcc
     4981 gcggcaccgt caccacccct gccatgcgcg tcagcgagga caacagcgcc gaagccgagc
     5041 ccaccacgtc gccgtcgccg ctgcgcaccg cgtccatctc cgcctgcagc cggcggatgg
     5101 ccgcgtccga cagcggctgc accttcagca acgcgtcgac gaacagccgg atgcccttcg
     5161 gcgtcggcac gcggccggcc gaggtgtgcg gcgcaaccac cagccccatc tcttcgaggt
     5221 ccgccatgac gttgcggatg gtcgccggcg acaggtcgag cccggaatcc ttcgacaggg
     5281 tgcgcgagcc gaccggctgg ccgtcacgga tatagcgctc caccagcacc ttcaggaggt
     5341 gccgggcgcg ctccgagagt tcggcttcga ccggtttatt cgccatactc gggattctac
     5401 gacggcgcgc gggccgacgc attccatgcc ccggcggcgc gatttgcccg ctggcccgcc
     5461 ccgcggcttc atgctacaag gacgcggccg cgccgctgaa tcgaaaccgg atgaaacgaa
     5521 gcttccacac catcgccctg accggcaaat accgggatcc gcgggtcggc gactcgatgt
     5581 tgatcctcgc gcgccacctc gccgccgcgg gcgtgcgggt gctgctggac cccgagatcc
     5641 gcatggactt tccgggcacc gcggtggagt cgctgcccga gggcgagctg gcggacgccg
     5701 ccgacctggt ggtcgcggtc ggcggcgacg gcaccatgct gtatgcctcg cgcctggtgg
     5761 ccgggcgcga cgtgccgctg ctcggcatca accgcggccg gctgggcttt ctcgccgaca
     5821 tcacgccggg cgagatgctg cgccggctgg acgaggtgtt ggccggcgac tacgacgaag
     5881 agcgccgcct gatgctggag gctgtcatcg accggggcga cggcgcgccg cgccgcgcgc
     5941 tggccctgaa cgacgtggtg ctgcaaaagc gcgacaccgg ccgcatgctc gatttcgaga
     6001 actgggtgaa cggcgtctac gtcaacaccc acggcggcga cggcctggtg atcgccaccc
     6061 cgaccggctc caccgcctac gcgctgtccg gcggcgggcc gatcatccac ccctcgctgg
     6121 atgccatcac gctggtgccg atctgcccgc acaccctgtc ggaccgcccg atcgtcatcc
     6181 gcggcgacgc gcgcatcgag gtgcgcgtgc tggagcgacc cgacacgcgc gccgaggtag
     6241 cttgcgacgg ccagccgctg ggcgagctgg cggccggcga acggctactg gtgcaggccg
     6301 ccgccgagcg cgtgctgctg attcacccgc gcggtcatga ctacttccgt ctgttgcgct
     6361 ccaagctgca ctgggggcgc ggcagccaga gccgcttcga cggcacctcc gaagcctgag
     6421 tcgccgccat gctgtccacc gtctcggtcc gcaatttcgc gatcatcgac gaagtgtcgc
     6481 tggagctggg tcccggcctg acggtgctta ccggcgagac cggcgccggc aagtcgatcc
     6541 tggtggacgc gctgggcctg gcgctcggcg accgcgccga cgcatcggcc gtacgccacg
     6601 gcgccgagcg cgccgagatc accgccagct tcgacctggc gacgctgccc gaggtagcgg
     6661 cctggctcac cgagcaggcg ctggacgcgg acggtgaatg cgtgttgcgg cgggtagtcg
     6721 gcgcggaggg gcgctcgcgt gcgtggatca acggcgcgca gaccaccctg cagacgctgc
     6781 gtgaactggg tgagcggctg gtggacatcc acggccagca cgagcaccag tcgctgacgc
     6841 gccccggcgt gcagcgcgaa ctgctggatg cgcggctgcc cgagcccgcg ccggcgcgcg
     6901 cggtggccga ggcctggcag gcctggcgcg tagcgctgga cgaacgccgg cggctggagg
     6961 gcgcggggcg tgaccgcgaa cagcggctcg acctgctgcg ctaccagcac cgcgaactgg
     7021 aggccttcgc gccgcgggcc ggcgaggccg ccgagctggc ggcggaattc ctccagctgg
     7081 ccaacgccgg caagctcgcc gagggcgccg gcggcgcgct ggagcgcgtc tacgagggcg
     7141 agtccggcgc ggcgcacgac gccgtggcgc aggcggccga ggcgctcgcc gagttggccg
     7201 agctcgaccc gcggctggcg gcgccccagc gcctgctggc ggaggccgaa atcctgctcg
     7261 ccgaggccgc caccgagctc aggcgctacc tcggcaacct ggacatggac ccggcgcggc
     7321 tggacgcggt gcaatcgcgg ctggaagaca tgaagagcct ggcgcacaag caccaggtcg
     7381 tggccgaggc gctgccggaa cggcttgctg cgctggccgc cgagatcgag gagctggagc
     7441 aggccgaggt gcggctcggc cgttcagcgg agaagctggc cgcagccgaa gcggagtacc
     7501 gcaaggccgc ggcgaagctc ggcaaggcgc gcgccgccgc ggccagggag ttttccgccc
     7561 aggtatcggc gctgatgcac caactcggca tgccgggcgg acgcttcgtg gtcgccgtcg
     7621 agcacgaccc gcaggccgag tgcgcgtccc acggcctcga cagcatcgag ttcctggtca
     7681 gcgccaaccc gggccagccg ccggcgccgg tctcgaaggt tgcctcgggc ggcgagctgt
     7741 cgcgcatcag cctcgccatc caggtggcgg ccaagagcga ccatcccatt cccgtgatgg
     7801 tgtttgacga agtggactcg ggcgtgggcg gcggtgtcgc agaaatcgtc gggcggcgcc
     7861 tggccgagat cggcgagcgc gcgcaggtgc tgtgcgtcac ccacctgccg caggtggcca
     7921 gccaggcgcg ccatcacctc cgggtggtca agctgaccga cggcgaagtg acgcgcacca
     7981 ccctcaaggc gctcaccgag gcggaaaagg tcgaggagct ggcgcggatg ctgggcggcg
     8041 tcgagatcac cagcacgacg cgcgcgcacg ccagggaaat gcgcaagcgc g
//
LOCUS       DAOMPG010000103         7998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3891_length_7998_cov_3.275211, whole genome shotgun sequence.
ACCESSION   DAOMPG010000103 DAOMPG010000000
VERSION     DAOMPG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3891_length_7998_cov_3.275211"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLS34_07045"
     CDS             complement(<1..852)
                     /locus_tag="PLS34_07045"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRP87259.1"
                     /translation="MVDLMAQESGAEIRRLMAGIGRQARIAAAALAQTSGAARDAALQ
                     GAAAMLRGRAERILAANAEDLATARDRGLSGALLDRLALDAARIEAMARGIEEIRALP
                     DPLGRVLAEWTRPNGLLIRRVSVPLGVIGIIYESRPNVTADAGALCLKSGNAVILRGG
                     SESFASSRAIHACLADALDDAGLPREAVQMVPTRERAAVGELLGGLGGCVDVVVPRGG
                     RNLIERVQRDARVPVIGHLEGICHVYVDGAADLAMARAIVLNSKMRRTGICGAAETLL
                     VDRACAAT"
     gene            complement(845..2008)
                     /gene="proB"
                     /locus_tag="PLS34_07050"
     CDS             complement(845..2008)
                     /gene="proB"
                     /locus_tag="PLS34_07050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRP87260.1"
                     /translation="MLSTVNDVNSSLAPASRIAAARRVVIKIGSALLVDRASGRLDAP
                     WFEDLVSDIARLRRRGQEVLVVSSGAIALGRGHLGLRRGALRLEESQAAAAAGQIRLA
                     HAWQEALGRHEVPVAQVLLTLGDTEERRRYLNARGTLETLLGLGAVPVINENDTVATE
                     EIRYGDNDRLAARVAQMVSADCLLLLSDTDGLHTADPTRDPAARLVEEVHAVTPEIEA
                     MAGEAATEFGKGGMITKVAAGRIALSAGCHMVIASGRVRHPVRAVEEGARCTWFIASV
                     SPQRVRKQWIAGSLQPRGEIVVDAGAAAALAAGKSLLPAGVIRVSGEFERGDAVLIRD
                     TGGRTLAHGLSAYGAADAARIIGLRSAQIEAALGYRGREELVHRNDLAMVTHG"
     gene            complement(2116..3306)
                     /gene="metB"
                     /locus_tag="PLS34_07055"
     CDS             complement(2116..3306)
                     /gene="metB"
                     /locus_tag="PLS34_07055"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRP87261.1"
                     /translation="MKTPAPATRAVRAGLGIDAEHGAVVPPIHLSSNFTFEGYGRKRR
                     YDYTRSGNPTRDALGEALAALEGGAGSVVTCSGMAAITLVCQLLGPEDLLLAPHDCYG
                     GTHRLLSSLAARGQFRLAFVDQTDAPALAAAFAGRPRMVLVETPSNPLLRITDLDAVA
                     RLCRAQGTLLVADNTFLSPGWQTPFAHGADIVLHSTTKYLNGHSDVVGGAVVARTPEL
                     HEQLDWWANCLGLTGAPFDSFLTLRGIRTLPARLRAHAENAAALVEVLAAHPAVERVH
                     YPGLETHPGHAIAARQQKGFGGMVSFELAGGEPAVEAFLDGLEHFFLAESLGGVESLV
                     AHPATMTHAAMDPEARRIAGIGDSLLRLSVGIEDPADLVADIDAALERAARVLERPLA
                     VAAG"
     regulatory      complement(3428..3558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3593..4681)
                     /gene="leuB"
                     /locus_tag="PLS34_07060"
     CDS             complement(3593..4681)
                     /gene="leuB"
                     /locus_tag="PLS34_07060"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRP87262.1"
                     /translation="MRGLITLLPGDGIGPEVTAAARGVLEVVARRFGHQFEFREALIG
                     GCAIDAAGEPLPPATVAACRAADGVLLGAVGGPQWSDPSAPVRPEQGLLALRRAMGVF
                     ANLRPVVTHRALYAASPLKESLLEGVDILFVRELTGGIYFGDKSRSADAASDLCVYSR
                     AEIERVVRLACTLARARRGVVTSVDKANVLETSRLWRETATRIARDEFPDVRLEHALV
                     DAAAMQLIQAPGRFDVVVTENMFGDILTDEASVITGSIGMLPSASLADRGPGLYEPIH
                     GSAPDIAGCGVANPCGAILSAAMLLRHSLGLEAEARAVEDATWDTVARGIGTADVVGK
                     HGAARGTDEVAAAIAAALEEAPAARAIA"
     gene            complement(4669..5253)
                     /gene="leuD"
                     /locus_tag="PLS34_07065"
     CDS             complement(4669..5253)
                     /gene="leuD"
                     /locus_tag="PLS34_07065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRP87263.1"
                     /translation="MEPFSVLTSRTAVLPVDNVDTDQIIPARFLTTTQSSGLGRHLFA
                     DWRRDADNQPRADFVLNRPENAGAKILVAGHNFGCGSSREHAPWALLDYGFRAVVSTG
                     IADIFRSNALRNGLLPVVLDAEPHGWLLANPGAELTIDLGRCVLCLPDGRAIPFEVEP
                     FARHCMLHGVDPLGWLLSREDDIRRYEEARTCAA"
     gene            complement(5253..6695)
                     /gene="leuC"
                     /locus_tag="PLS34_07070"
     CDS             complement(5253..6695)
                     /gene="leuC"
                     /locus_tag="PLS34_07070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRP87264.1"
                     /translation="MSEATPGAQGPRTLFQKVWDRHLVVPESADTPAVIYIDLHLVHE
                     VTSPQAFAVLRERGLKVRRPERTLATVDHSIPTDAAAWRSGLVDMDHAARHQVRMLGD
                     NCGRAGVRLFGVNSSRRGIVHVIGPEQGATQPGMTIVCGDSHTATHGAFGALAFGIGT
                     TEVGHVLATQCLLQRRPRTLRIEIAGRLQPGVTAKDVILAVIGRIGVGGGTGHVIEFC
                     GAAVSAMSMDERMTLCNMAIEAGARAGMVAPDDTTFEYLAGRPLAPQGAAWDRAVAGW
                     RSLAADPGAAYDRSVTIDVAELEPQITWGTDPGMVIPVSGRIPAPAGAGHRKALEYMG
                     LQAGAPIAGQPVQIVFIGSCTNSRISDLREAAALLRGRRVARGVRALVVPGSQQTRAM
                     AEAEGLDRVFIDAGAEWHQSGCSLCIAMNGDRAGAGQYVVSTSNRNFEGRQGQGARTL
                     LASPATAAAAAVAGCVADPRALLESERRIA"
     gene            complement(6692..>7147)
                     /locus_tag="PLS34_07075"
     CDS             complement(6692..>7147)
                     /locus_tag="PLS34_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRP87265.1"
                     /translation="QLISMEAKRHGYAEGIGLTTDGMIGEGAGENIFIVKDGKIYTPP
                     AACSILAGITRDTVITLARDLGYEVHEQPMPREMLYFADEVFFSGTAAEVTPVRSVDR
                     IQIGAGSRGPVTEAVQERFFGLFNGRTRDQWGWLQPLESRVATMASVAV"
     assembly_gap    7148..7157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7158..7661)
                     /locus_tag="PLS34_07080"
     CDS             complement(<7158..7661)
                     /locus_tag="PLS34_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRP87266.1"
                     /translation="MAPKFPRYIWQNGRLVPGENATVHVLSHALHYGSSVFEGVRVYK
                     TPEGSRSFRLREHITRLFNSAKLYRIEIPYSQAEIEAACQHAVLANDLDNGAYIRPVI
                     FRGFAGLGLTAPAGSPVEVAIAAIEWGAYLGAEGLKNGVDVCVSSWQRVAPNTIPSGA
                     KAAGGYLS"
     gene            complement(7701..>7998)
                     /locus_tag="PLS34_07085"
     CDS             complement(7701..>7998)
                     /locus_tag="PLS34_07085"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRP87267.1"
                     /translation="IHADGHACREAGTGDGPVEAAFRAMERATGIEVELKSFDIRNVT
                     VGEDAQGEAVVSVLHGGRTWQGKGFDTDIIQASAQAFLQAINRIAARQGADAAA"
BASE COUNT         1132 a         2945 c         2794 g         1117 t
ORIGIN      
        1 agtcgcggcg caggcccggt ccaccagcag ggtctcggcg gccccgcaga tgccggtgcg
       61 ccgcatcttc gagttcagca cgatggcccg cgccatcgcc aggtccgccg cgccgtccac
      121 atacacgtgg cagatgccct cgaggtgccc gatgaccggc acgcgggcgt cgcgctgtac
      181 ccgctcgatg agattgcgac cgccgcgcgg cacgaccacg tccacgcagc cgccgaggcc
      241 gccgagcagt tcgccgaccg ccgcccgctc ccgagtcggc accatctgca cggcttcgcg
      301 cggcagcccg gcgtcgtcca gggcgtcggc caggcaggca tggatggccc ggctcgaggc
      361 gaagctctcc gagccgcccc gcaggatcac cgcgttgccg gacttcaggc acaaggcccc
      421 ggcgtcggcg gtcacgttcg gccggctctc gtagatgatg ccgatgacgc ccagcggcac
      481 gctgacgcga cggatcagca ggccgttggg gcgggtccac tcggccagca cgcgtcccag
      541 cgggtccggc agtgcccgaa tttcctcgat cccccgggcc atcgcctcga tgcgcgccgc
      601 atcgagcgcc agccggtcga gcagcgcccc ggacaggccc cggtcccggg cggtcgccag
      661 gtcctcggca ttggcggcca ggatccgctc cgcccggccg cgcagcatcg cggcagcacc
      721 ctgcagggcg gcatcgcgcg ccgcaccgct ggtctgcgcc agcgcggccg cggcgatacg
      781 cgcctgccgc ccgatgccgg ccatcaggcg ccggatctcc gcacccgatt cctgcgccat
      841 caggtcaacc atgcgtcacc atcgccaggt cgttgcgatg caccagctcc tcgcggcccc
      901 ggtagccgag cgccgcctcg atctgggcgc tgcgcaggcc gatgatacgc gccgcatcgg
      961 cggcgccgta ggccgacagg ccgtgcgcca gcgtccggcc gcccgtatcg cgaatcagca
     1021 ccgcgtcgcc gcgctcgaac tcgccgctga ctctgatgac gccggccggc agcaggctct
     1081 tgcccgccgc cagcgcagcc gccgcgccgg cgtcgacgac gatctcgccg cgcggctgca
     1141 gcgaaccggc gatccactgc ttgcgcaccc gctgcggact caccgaagcg atgaaccagg
     1201 tgcagcgcgc gccctcttcg accgcccgca ccgggtggcg gacgcggccc gaggcaatca
     1261 ccatgtggca gccggccgac agcgcgatgc ggcccgcggc aaccttggtg atcatgccgc
     1321 ccttgccgaa ctcggtcgcc gcctcgccgg ccatggcctc gatctccggc gtcacggcat
     1381 gcacttcctc caccaggcgc gccgccgggt cgcgggtcgg gtccgcggtg tgcaggccgt
     1441 cggtgtcgga caacagcagc aggcagtcgg cgctgaccat ctgcgccacc cgcgcggcga
     1501 gccggtcgtt gtcgccatag cgaatctctt cggtggcgac cgtgtcgttc tcgttgatca
     1561 ccggcacggc gccgagcccg agcaaggtct cgagcgtgcc gcgggcgttc aggtagcggc
     1621 ggcgctcctc ggtgtcgccg agcgtcaaca gcacctgcgc gacgggaacc tcgtgccgtc
     1681 cgagggcctc ctgccaggcg tgcgcgaggc ggatctgccc ggccgcagcc gccgcctggc
     1741 tctcttccag gcgcagcgcg ccgcggcgca ggccgaggtg gccgcggccc agtgcgatgg
     1801 cgccggagga caccaccagc acctcctggc cgcgccgccg cagcctggcg atgtcgctga
     1861 ccaggtcctc gaaccagggc gcgtcgagcc ggccgctggc ccggtccacc agcagcgccg
     1921 agccgatctt gatcacgacc cggcgtgccg ccgcgatgcg gctggccggc gccagactgc
     1981 tattgacgtc gttcactgtc gagagcatga ccgttgcgcc gcaacatgtg aagtcccggg
     2041 accggcgaaa ccgggcaaaa ccggggtcca aaaccggggt cagaccccgt tttcgggaaa
     2101 cggggtctga ccccattaac cggccgccac cgccagcggg cgctccagca cgcgcgctgc
     2161 ccgctcgagc gccgcgtcga tatcggcgac caggtcggcc gggtcttcga tcccgacgga
     2221 gagccgcagc aggctgtcgc cgataccggc gatccggcgg gcttccgggt ccatggcggc
     2281 atgggtcatg gtggccgggt gcgccaccag gctctccacg ccgcccagcg actcggccag
     2341 gaagaaatgc tccagcccgt cgaggaaggc ctccacggcc ggctcgccgc cggccagctc
     2401 gaagctcacc atgccgccga agcccttctg ctggcgcgcg gcaattgcgt ggccggggtg
     2461 ggtctccagc cccggatagt gcacccgttc caccgccggg tgtgccgcga gcacttcgac
     2521 cagtgccgcg gcgttctcgg catgcgcgcg cagccgggcc ggcagcgtgc ggatgccccg
     2581 cagcgtcagg aagctgtcga agggcgcgcc ggtcaggccc aggcagttgg cccaccagtc
     2641 gagctgctcg tgcagctcag gggtcctcgc caccaccgcg ccgccgacca cgtcgctgtg
     2701 gccgttgagg tacttggtgg tcgagtgcag cacgatgtcc gcgccgtgcg cgaaaggagt
     2761 ctgccagccg ggcgacagaa aggtattgtc cgccaccaac agcgtgccct gcgcgcgaca
     2821 cagccgcgcg accgcgtcca ggtcggtgat gcgcagcagc gggttgctcg gcgtctcgac
     2881 cagcaccatg cgcgggcggc cggcaaacgc agccgccagc gccggcgcat cggtctggtc
     2941 cacgaaggcg agccggaact ggccgcgcgc cgccagcgag gacagcagcc ggtgcgtgcc
     3001 gccgtaacag tcgtgcggcg ccagcagcag gtcctccggt ccgagcaact ggcagaccag
     3061 cgtgatcgcc gccatgccgg agcaggtcac caccgagccg gcgccgcctt ccagcgcggc
     3121 cagcgcctcg ccgagcgcgt cgcgcgtggg attgccggac cgcgtgtaat cgtagcggcg
     3181 cttccggccg tagccctcga aggtgaagtt cgagctgagg tggatcggcg gcaccacggc
     3241 gccgtgctca gcatcgatgc cgagcccggc gcgcacggcg cgggtcgcgg gggcgggtgt
     3301 cttcatctgc tctcctccgt cgtgcgccct tgcgggagcg ggttccgccc atcgggaacc
     3361 ctgcggaggc aatcgggggg aagtcgccgg acccaatcac ccgcctctcc cggcggggtt
     3421 gcccggcaac tcatctcccg gtgaacgcgc ggcgcgagcc gcaggtcctc cgcaggaatt
     3481 ggcaccgtcg cgaagtggtg agcttcgcag gttgccccgg catcaaaggg cctgtccctc
     3541 cgccggtctc gatgagtgct tactgtgtac tcttctaact catccggccg cttcaggcaa
     3601 tcgcccgggc cgccggcgcc tcctccaaag ccgctgcgat ggccgcggcc acttcgtcgg
     3661 tgccgcgcgc ggcgccatgc ttgccgacca cgtcggcggt gccgatgccg cgcgccacgg
     3721 tgtcccaggt cgcgtcctcg acggcgcgcg cctcggcttc gaggccgagc gaatggcgca
     3781 acagcatcgc ggcgctgaga atggcgccgc agggattcgc gacgccacat cccgcgatgt
     3841 ccggcgccga gccgtggatg ggttcgtaca ggcccgggcc gcggtccgcc agcgacgccg
     3901 acggcagcat gccgatggag ccggtgatca ccgaggcctc gtcggtgaga atgtcgccga
     3961 acatgttctc ggtgaccacc acgtcgaagc ggcccggagc ctggatcagc tgcatggccg
     4021 ccgcgtccac cagtgcatgc tccagccgca cgtcgggaaa ctcgtcgcgc gcgatgcgcg
     4081 tggccgtttc ccgccacagc cgcgaggtct ccagcacgtt ggccttgtcc accgaggtca
     4141 ccacgccacg ccgcgcgcgc gccagcgtgc aggccagccg taccacacgc tcgatctccg
     4201 cccggctgta gacgcagaga tcggaggccg catcggcgga gcgcgacttg tcgccgaaat
     4261 agatgccacc ggtcagctcg cgcacgaaca ggatgtcgac gccttccagc agagactcct
     4321 tgaggggcga agccgcgtac agcgcccgat gcgtcaccac cgggcgcagg ttggcgaaca
     4381 cgcccatggc ccgccgcagc gccagcaggc cctgctcggg gcgcacgggg gcggacgggt
     4441 ccgaccactg cgggcccccg accgcaccga gcagcacgcc gtcggctgcg cggcaggccg
     4501 cgacggttgc cggcggcagc ggctcgcccg cagcgtcgat ggcgcagccg ccgatgagcg
     4561 cctcgcgaaa ctcgaactgg tggccgaagc gccgcgccac gacctcgagc acgccgcgtg
     4621 cggcggccgt gacctcgggc ccgatgccgt cgcctggcag cagggtgatc aggccgcgca
     4681 tgtgcgcgcc tcctcgtagc gccggatgtc gtcctcgcgg ctcagcagcc agcccagcgg
     4741 gtccacgccg tgcagcatgc agtggcgcgc gaagggctcg acctcgaacg ggatggcgcg
     4801 gccgtccggc aggcacagca cgcagcggcc gagatcgatg gtgagctcgg cgccgggatt
     4861 ggccagcagc cagccgtgcg gctcggcgtc gagcaccacc ggcaacagcc cgttgcgcag
     4921 ggcattgctg cggaagatgt ccgcgatgcc cgtgctgacc actgcgcgga agccgtaatc
     4981 gagcagggcc cagggcgcat gctcgcgcga cgagccgcag ccgaagttgt gcccggcgac
     5041 caggatcttc gcgccggcgt tctccgggcg gttgagcacg aagtcggcgc gcggctggtt
     5101 gtcggcatcc cgtcgccagt cggcgaacag gtggcggccc agcccgctgc tctgcgtggt
     5161 ggtgagaaac cgcgcgggaa tgatctggtc ggtgtccacg ttgtccaccg gcagtaccgc
     5221 ggtacgcgaa gtcagcacgg agaacggttc gatcatgcga tgcgcctctc gctctccagc
     5281 agcgcgcggg gatccgccac gcagcccgcg accgccgcgg ccgccgccgt ggccgggctg
     5341 gccagcaggg tgcgcgcgcc ctggccctgg cgaccctcga aattgcggtt gctggtgctc
     5401 actacgtact gtccggcgcc ggcgcgatcg ccgttcatgg cgatgcacag cgaacagccg
     5461 gactggtgcc actcggcacc ggcatcgatg aacacgcgat ccagcccctc ggcctccgcc
     5521 atcgcccggg tctgctggct gcccggaacc accagcgcgc gcacgccgcg cgccacgcgc
     5581 cggccgcgca gcaacgccgc cgcctcgcgc aggtcgctga tccgcgaatt ggtgcagctg
     5641 ccgatgaaca cgatctgcac cggctggccg gcaatgggcg cgcccgcctg caggcccatg
     5701 tactcgagcg ccttgcggtg cccggcgccg gccggcgccg gaatgcgtcc gctcaccggt
     5761 atcaccatgc ccgggtcggt gccccaggtg atctgcggtt cgagctctgc gacgtcgatg
     5821 gtgacgctgc ggtcgtaagc ggcgccggga tccgcagcca ggctgcgcca gccggccacg
     5881 gcacgatccc aggccgcgcc ctgcggggcc agcgggcgac ccgcgaggta ttcgaaggtg
     5941 gtgtcgtcgg gggccaccat gccggcgcgc gcgcccgcct cgatcgccat gttgcacagg
     6001 gtcatgcgct cgtccatgga catcgcgctg acggccgcgc cgcaaaattc gatgacgtgt
     6061 cccgtgccac cgccgacgcc gatccgtccg atcaccgcga ggatgacgtc cttggccgtc
     6121 acgccgggct gcaggcggcc ggcgatttcg atacgcaggg ttctcgggcg gcgctgcagc
     6181 aggcactgcg tggccagcac gtggccgacc tcggtggtgc cgatgccgaa ggccagcgcg
     6241 ccgaaggcgc cgtgggtggc ggtgtggctg tcgccgcaga cgatggtcat gccgggctgc
     6301 gtggcgccct gctccggccc gatgacgtgc acgatgccgc gccgcgagct gttgacgccg
     6361 aacaggcgga cgcccgcgcg gccgcagttg tcgcccagca tgcggacctg gtggcgcgcc
     6421 gcgtggtcca tgtcgaccag gccgctgcgc caggccgcgg cgtcggtcgg aatcgagtga
     6481 tcgaccgtgg ccagcgtccg ctcggggcgg cgcaccttta ggccgcgctc gcgcagcacg
     6541 gcaaaggcct gcggcgacgt gacctcgtgc accaggtgga ggtcgatgta gatcaccgcc
     6601 ggcgtgtcag cggactcggg cacgaccagg tgccggtccc agactttctg gaacagcgtg
     6661 cgcggtccct gcgccccggg cgtcgcctcg ctcatactgc caccgacgcc atcgtggcca
     6721 cgcgcgactc cagcggctgc agccagcccc actggtcgcg cgtccggccg ttgaacaggc
     6781 cgaagaagcg ctcctgcacg gcctcggtga ccgggccgcg cgagcccgcg ccgatctgga
     6841 tacgatccac cgagcgcacc ggcgtgactt ccgctgcagt gcccgagaag aacacctcgt
     6901 cggcgaaata cagcatttcg cgcggcatcg gctgctcgtg cacctcgtaa ccgaggtcgc
     6961 gggccagcgt gatgacggtg tcgcgcgtga tgccggccag gatcgagcag gcggcaggcg
     7021 gcgtgtagat cttgccgtcc ttgacgatga agatgttttc gcccgcgccc tcgccgatca
     7081 tgccgtcggt ggtcaggccg atgccctcgg catagccgtg gcgcttcgcc tccatgctga
     7141 tcagctgnnn nnnnnnncga caggtagccg cccgccgcct tggcgccgga cgggatggtg
     7201 ttcggggcga cgcgctgcca ggaggacacg cacacgtcca cgccgttttt cagcccctcg
     7261 gcgccgaggt aggcgcccca ctcgatggcc gcgatggcga cctccaccgg cgagccggcc
     7321 ggcgccgtca ggccgagtcc cgcgaagccg cggaatatca ccggccggat gtaggcgccg
     7381 ttgtccaggt cgttggccag caccgcgtgc tggcaggccg cctcgatctc cgcctggctg
     7441 tagggaatct cgatgcgata gagcttggcg gaattgaaca gccgcgtgat gtgctcgcgc
     7501 aggcggaacg accgcgatcc ctccggtgtc ttgtagaccc gcaccccctc gaataccgac
     7561 gagccgtagt gcagggcgtg cgacagcacg tgcaccgtcg cgttctcgcc ggggaccagc
     7621 ctgccgttct gccagatgta gcggggaaac ttgggcgcca cttgaattct ccgttgcctg
     7681 tcgcgaattg aagctgtgga tcaggccgcc gcgtcggcgc cctggcgggc ggcgatgcgg
     7741 ttgatggcct gcagaaaggc ctgggcgctg gcctggatga tgtccgtgtc gaagcccttg
     7801 ccctgccagg tccggccgcc gtgcagcacg ctgacgaccg cctcgccctg ggcgtcctcg
     7861 ccgaccgtga cgttgcggat gtcgaagctc ttcagctcga cctcgatgcc cgtggcgcgc
     7921 tccatggcgc ggaacgcggc ctcgaccggg ccgtcccccg tgccggcttc ccggcaggca
     7981 tggccgtcgg cgtgaatc
//
LOCUS       DAOMPG010000104         7830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_3989_length_7830_cov_4.957299, whole genome shotgun sequence.
ACCESSION   DAOMPG010000104 DAOMPG010000000
VERSION     DAOMPG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3989_length_7830_cov_4.957299"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="PLS34_07090"
     CDS             complement(<1..1392)
                     /locus_tag="PLS34_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRP87268.1"
                     /translation="MRLAALRLVLGLAAVGGACAAIEAAAAERAADAAPVVVERDLEA
                     SLDGATLRQVEILRLLIQAARIMDTLYLEQVEGEGFYPADMSREEFDAWRDPAAADPH
                     TVVRRDAAGALQAVPYHEAWPQELGYAARLLAQAAEISTDESLRNYLTQRARALITGD
                     YARAEAAWRAMRYSDLDVLIGPILRDADRRFGLKAGFGAYVMLRDWGWGARLAAFTVF
                     LPRVQASLPVADSFKAEVPEVDMKVAVYDLLFHAGYGVPRADSVTPEELGEPRLRIRQ
                     GPQRLQLRNVTQARFDALVQPVADALIAPEQHGHVRFDPFFLNTMFHEMAHALGMRET
                     VTARGPVNAALREHADTIEELKAAVLSLWLANWLNANGAMRGTAPMDHYASLLAAVFR
                     AVHIDADSATGRAHMMLFNYFRDWGAFRRDAATGRYRVDAADIGPAVAALAAQVLTIQ
                     GSGDYEGATELIRA"
     gene            complement(1400..1867)
                     /locus_tag="PLS34_07095"
     CDS             complement(1400..1867)
                     /locus_tag="PLS34_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87269.1"
                     /translation="MRPARLAFVTACVLACTAHGAWACNPDPPRPPILQGFSYDAAAA
                     EVLLRDATSVVAARLELELALQDSAGVARSDFVFAVLEGWRAETPRTLTIGGHWVDCA
                     IEPRQGRVFLLYLDGQRLLHAVPVSALDFEIALLGEPDWFYDVRGRLVRGPED"
     gene            complement(1891..3357)
                     /locus_tag="PLS34_07100"
     CDS             complement(1891..3357)
                     /locus_tag="PLS34_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP87270.1"
                     /translation="MRQRQDPEGLRLPVKLDSTSNGEFEPVPLEPQHRHANRLALDHA
                     TANAKRLGLSRRDFLVSASGAATTLLAMNAAYAAVGNTGGFFQLDPEASLDLAMAENA
                     LGGDEFIFDVQGHFVNPTGDWTRRLPEGARPLGSFSTQGCAAAELPGPLDRLQCFGPD
                     AFIRDVFLDSDTSLVVLSFVPSTREGEPLTIEEAAATAAIVERLEGSHRLYLHGRVNP
                     NQPGDLEDMERLATDYGISAWKTYTQWGPDGAAGFWMDDEPGLAMIENARRLGVPTIA
                     IHKGLPFGQRSYEHSTCADVGRVGKRFPDVNFLIYHSGFVTGQAEGPYDPARADGIDA
                     LVTSVLEAGLGHGSNVYAELGSTWRFLMRDPTSAAHGLGKLLKYLGPDNVLWGTDSIW
                     YGSPQDQIQAFRSFQIDPELRERHGYPEITPEIRAKVFGRNALQIYPVPEDLLGEHLR
                     RDRVARERENYRNRPDPAYLTYGPKTRREFLNLKAWGG"
     gene            complement(3421..4269)
                     /locus_tag="PLS34_07105"
     CDS             complement(3421..4269)
                     /locus_tag="PLS34_07105"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRP87271.1"
                     /translation="MRTLGDFLGDAVDTLTNVNGKLLKSLRLLLFRPGFLSAEHARGA
                     RVPYVRPVQLFVVANLAFFLWSYVPIFSTPLQVHTWSQGFPHRAVAERWVREAVAPDM
                     TADEFRAVLLQAWSPFTTSTPAVDVPPATLAAMEALAQQFNARVELQGRALVVLMIPL
                     FAIWPGMLNLRRRRHAIEHLVFATHFMSLVLLAALAQMWLMRSIHAALQSLAGVDVAL
                     LQSDPFNSMVLGLLMAAWLAAAVRVYYRRSWPAAVLQCLALVVLFLLSTQVFRAVLFF
                     TTWWSL"
     gene            complement(4429..4632)
                     /locus_tag="PLS34_07110"
     CDS             complement(4429..4632)
                     /locus_tag="PLS34_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87272.1"
                     /translation="MPGLDEHQRQLVEQKRAEFEARAAQQDAFADSAFPEGAQLCAKC
                     NTTAVVMMDGCMTCLNCGDSKCG"
     gene            5251..5817
                     /locus_tag="PLS34_07115"
     CDS             5251..5817
                     /locus_tag="PLS34_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87273.1"
                     /translation="MTAIFATATLGLLIPSTGMAGNGPTVTVNPDLQQAIMFLDGGEK
                     ISMNVLTQVFTFRAASGQSVSFTFAEVAQEITANPSEQQALIQQWSTEVSDPSNRFTI
                     TNTHEPTLSVFSGPPNPPPGYFEIEGAPGIGGSNLPKSPVNGSFSSLQGGSTTPGPCS
                     FASCSCKGGECWPSHGFPVEYGHDLLFD"
     gene            6375..6629
                     /locus_tag="PLS34_07120"
     CDS             6375..6629
                     /locus_tag="PLS34_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP87274.1"
                     /translation="MRGTTRQDPASTTVQDVKSEKVLYCFKGDDVADAAKSMRKQQVY
                     RLTVLDERDNKKLCGVIPLGDILRHKQGELAENTADAIVA"
     gene            complement(6727..7428)
                     /locus_tag="PLS34_07125"
     CDS             complement(6727..7428)
                     /locus_tag="PLS34_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HRP87275.1"
                     /translation="MSAHKIDKKIVAYHVAKPEEKAPPPKPRVEEGTEGKVIRMHEKL
                     ERPDMLIGATYKIKPPVAEHAMYVTINDILLNAGTKHEARRPFEIFINSKNLDHYQWI
                     VALTRIMSAVFRKGGDVTFLVEELKAVFDPRGGYWKPGGKYMPSLIAELGEVVEKHLV
                     SIGMMQAPGLDEHQRQLVEQKRAEFEARAAQQDAFADSAFPEGAQLCAKCNTTAVVMM
                     DGCMTCLNCGDSKCG"
     gene            complement(7438..>7830)
                     /locus_tag="PLS34_07130"
     CDS             complement(7438..>7830)
                     /locus_tag="PLS34_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HRP87276.1"
                     /translation="EKLPDYFITAEDITPRQHVDIQAAAQMWVDSSISKTANVPTDYP
                     YEDFKDIYMYAYEQGLKGCTTFRFNPAAFQGVLVKEQDLKNTTYVFELEDGSTIELKG
                     DEEVEYDGEMHTAANLFDALKEGYYGKF"
BASE COUNT         1347 a         2519 c         2615 g         1349 t
ORIGIN      
        1 ggcgcggatc agctcggtgg cgccctcgta gtccccgctg ccctggatgg tcagcacctg
       61 ggcggccagc gccgcgaccg ccgggccgat gtccgccgca tccacccggt agcgaccggt
      121 ggccgcgtcc cgccggaagg cgccccagtc gcggaaatag ttgaacagca tcatgtgggc
      181 gcgcccggtg gcgctgtcgg cgtcgatgtg caccgcgcgg aacaccgcgg ccagcaggct
      241 ggcgtagtga tccatcggcg ccgtgccgcg catcgcgccg ttggcattga gccagttggc
      301 cagccacaag gacagcacgg ccgccttgag ttcctcgatc gtgtcagcgt gctcgcgcag
      361 cgctgcgttc accggcccgc gcgcagtcac cgtctcccgc atgcccaggg cgtgggccat
      421 ctcgtgaaac atggtgttga gaaagaaggg atcgaagcgc acatggccgt gctgctcggg
      481 cgcgatcagc gcatccgcca ccggctgcac cagcgcatcg aagcgggcct gggtcacgtt
      541 gcgcagctgc agccgctgcg gcccctggcg gatccgcagg cgcggctcgc cgagttcctc
      601 cggggtgacc gagtcggccc gcggcacgcc gtagccggca tggaacagca ggtcgtacac
      661 ggccaccttc atgtcgacct cgggcacttc cgccttgaag gagtccgcca ccggcaggct
      721 ggcctgcacg cgcggcagga acacggtgaa ggccgccagg cgcgcgcccc agccccagtc
      781 gcgcagcatc acgtaggcgc cgaagcccgc cttcaggccg aagcgccggt cggcgtcgcg
      841 caggatgggc ccgatgagca catccaggtc gctgtagcgc atcgcccgcc acgcggcctc
      901 ggcgcgcgcg tagtcgccgg tgatcagcgc ccgggcgcgc tgggtgaggt agttgcgcag
      961 gctctcgtcg gtgctgatct cggccgcctg cgccagcagc cgggcggcgt aaccgagctc
     1021 ctgcggccag gcctcgtgat agggcacggc ctgcagcgcg ccggccgcat cgcgacgcac
     1081 caccgtgtgc gggtccgcgg ccgccggatc gcgccaggcg tcgaactcct cgcggctcat
     1141 gtcggccgga tagaagccct cgccctccac ctgttcgagg tacaacgtgt ccatgatgcg
     1201 cgcggcctgg atcagcaggc gcaggatctc gacctggcgc agcgtcgcgc cgtcgaggct
     1261 ggcctcgaga tcccgctcca ccaccacggg cgcggcgtcg gccgcgcgct ccgccgccgc
     1321 ggcctcgatc gcggcgcagg cgccgccgac ggcggccagg ccgagcacca gccggagcgc
     1381 tgccagtcgc atggggccgc tagtcctccg gcccgcgcac cagccggccg cgcacgtcgt
     1441 agaaccagtc cggctcgccc agcagcgcga tctcgaagtc cagcgcgctc accggcaccg
     1501 cgtggagcag gcgctggccg tcgagataga gcaggaacac ccgcccctgg cgcggctcga
     1561 tggcgcagtc cacccagtgc ccgccgatgg tgagcgtgcg cggcgtctcg gcgcgccagc
     1621 cctccagcac cgcgaacacg aagtcgctgc gcgcgacccc ggcggaatcc tgcagcgcca
     1681 gctcgagttc cagccgcgcc gcgaccacgg acgtcgcatc gcgcagcagg acttcggccg
     1741 cggcggcgtc gtagctgaag ccctgcagga tgggcggccg cggcggatcc gggttgcagg
     1801 cccacgcacc gtgggccgtg caggccagca cgcaggcggt cacgaacgcg aggcgggcag
     1861 gtctcatgcc gcgattctaa cccgcgcggc tcagcctccc catgccttga ggttgagaaa
     1921 ctcgcggcgc gtcttcggcc cgtaggtcag gtaggccgga tcggggcggt tgcgataatt
     1981 ctcgcgctcg cgcgccacgc ggtcgcggcg caggtgctcg cccagcaagt cctctggcac
     2041 ggggtagatc tgcagcgcgt tgcggccgaa caccttggcg cggatttccg gcgtgatttc
     2101 cgggtagccg tgccgctcgc gcagctccgg gtcgatctgg aaactgcgga aggcctggat
     2161 ctggtcctgc gggctgccgt accagatcga atcggtgccc cacagcacgt tgtccggccc
     2221 caagtacttg agcagtttgc cgaggccgtg cgccgccgag gtcgggtcgc gcatcaggaa
     2281 acgccaggtc gagcccagct ccgcgtacac attgctgccg tgccccaggc cggcctccag
     2341 caccgaggtg accagcgcat cgatgccgtc ggcgcgcgcc gggtcgtagg gtccttcggc
     2401 ctggccggtg acgaagccgg agtggtagat gaggaagttg acgtccggga agcgcttgcc
     2461 gacccggccg acgtcggcgc aggtgctgtg ctcgtaggag cgctgcccga agggcaatcc
     2521 cttgtggatg gcgatggtgg gcacgccgag ccggcgcgcg ttctcgatca tggcgagacc
     2581 cggctcgtcg tccatccaga agcccgccgc gccgtcggga ccccactggg tgtaggtctt
     2641 ccaggcgctg atgccgtagt ccgtcgcgag ccgctccatg tcctcgaggt cgcccggctg
     2701 gttagggttg acccgcccgt gcaggtaaag ccgatggctg ccctcgagcc gttccacgat
     2761 ggcggccgtg gcggccgcct cctcgatggt gagcggttcg ccctcgcgcg tcgacggcac
     2821 gaaggacagc accaccagcg aggtgtcgga atcgaggaac acgtcgcgga tgaaggcgtc
     2881 cggcccgaag cactgcaagc ggtcgagcgg gccgggcagc tcggccgcgg cgcagccctg
     2941 ggtcgagaag ctgccgagcg ggcgcgcgcc ctccgggagg cggcgcgtcc agtcgccggt
     3001 cgggttcacg aagtggccct ggacgtcgaa gatgaactcg tcgccgccca gcgcgttctc
     3061 cgccatggcg aggtccagcg aggcttcggg gtccagctga aagaagccgc cggtgttgcc
     3121 gaccgcggca taggcggcgt tcatggcgag cagcgtggtg gcggcgccgc tcgcggagac
     3181 caggaaatcg cgtcgcgaca gtccgaggcg tttcgcgttg gcggtggcgt ggtccagcgc
     3241 gaggcggttg gcgtgccgat gctgcggctc gagcggcacc ggctcgaact cgccgttgct
     3301 ggtggagtcg agcttgaccg ggaggcgaag tccctcggga tcctggcgct gacgcatgac
     3361 gactctcctg cttgcgtgaa attgtgccgg cattgtaccc cggcacgcgc agcaggccgg
     3421 ctagagcgac caccaggtcg tgaagaacag caccgcgcgg aacacctggg tgctcaacag
     3481 gaacagcacg accagcgcca ggcactgcag cacggcggcg ggccagctac gccggtagta
     3541 gacccgcacg gccgccgcca gccaggcggc catcaacagc cccagcacca tggaattgaa
     3601 ggggtcgctc tgcagcaggg cgacgtcgac cccggccagt gattgcagag cagcatggat
     3661 gctgcgcatc agccacatct gtgcgagcgc cgccaacagc accaggctca tgaagtgggt
     3721 ggcgaacacc aggtgctcga tggcgtgccg tcgacgccgg aggttcagca tgccgggcca
     3781 gatcgcgaac aggggaatca tcagcaccac cagggcgcga ccctgcagct cgacgcgcgc
     3841 attgaattgc tgcgcaagcg cctccatggc cgccagcgtc gcgggcggca catccaccgc
     3901 cggcgtggac gtcgtgaacg gcgaccacgc ctgcagcagc actgcgcgga actcatcggc
     3961 ggtcatgtcc ggcgccaccg cctcgcgtac ccaccgctcg gcgacggcgc gatgcggaaa
     4021 gccttgcgac caggtgtgca cctgcagggg cgtgctgaag atcggcacgt aggaccagag
     4081 gaaaaacgcc aggttggcca ccacgaacag ctgtaccggg cgcacgtacg gcacgcgcgc
     4141 gccacgggca tgctcggcgg agaggaaccc gggacggaac agcagcagcc gcagggactt
     4201 gagcagcttg ccgttgacgt tggtcagggt gtcgacggcg tcgccgagga agtcgccgag
     4261 cgttcgcatg cgctcgtccg gcgcgcgctc gccgcaggcg gcacagtatg cgccgccaag
     4321 cggcgcaccg caggaaacgc aggaactcga tgtcggcatg cctggccccg gaaacgaacg
     4381 ggggcgaccg cctggccgcc ccctttagtc tagccgctca ggcagtcttc agccgcactt
     4441 cgagtcgccg cagttcaggc aggtcatgca gccgtccatc atcaccacgg cggtggtgtt
     4501 gcacttggcg cacagctgcg cgccctcggg gaaggcgctg tccgcgaagg cgtcctgctg
     4561 cgcggcgcgc gcctcgaact cggcgcgctt ctgctcgacc agctggcgct ggtgctcgtc
     4621 gaggcccggc aatcgtctga taggcggcgc cggcgccgca ggccgagcat caccgatccc
     4681 agggaacagg acaaccagga ggacatccat gaattgatga ccgacacgcc gcgctatgtc
     4741 accggcacat ctacgctgca ggacgctgcc cgctagatgg cggacctgga ctgcggcttc
     4801 ctgctaaccg cggatgagcg cgaaccatga caacggcggc aggtgcacag ctcggaagtg
     4861 tcagctgtac tgtggagaac ccaccacaca ttttcggacg tgaccgaaga atcggttttc
     4921 aacgctgatt ctactgaggc gcacacggca ttctctcggg acccttcaag catcgctcgt
     4981 ggccgccatg taatcaaaat ggcagtggcc gcaggctggc gaatgctcca cgctgagtca
     5041 cagtcggctg atttttttgt ggttcttgca cggtcagaca ccgagccgat gaattgcgcg
     5101 accatgcgtt ccagcgccgt caagtcgagt ttgacaggct gcagtttgtc tactgcgctc
     5161 tgaatatggg cgtttggtcc catatatagt cactgtgttg tcttgcgcaa gttttgtgca
     5221 cgaaaatgga ggggagcgat aattcgacag atgacggcaa tttttgcaac ggcaacactt
     5281 gggcttttga tcccttcgac cggaatggca gggaacggac ctactgtcac cgttaatcca
     5341 gacttgcagc aagcgattat gttcctggat ggtggcgaga aaatcagcat gaacgtattg
     5401 actcaagtgt tcacttttcg agcggcgagc ggtcagagcg tctcattcac gtttgcggaa
     5461 gttgcgcagg agataacggc aaatccgtcc gaacagcaag cattgattca acagtggagt
     5521 acagaagtta gcgatccatc gaatagattc actattacga acacgcacga gccgacctta
     5581 tcggtgtttt ccggtccgcc gaatccgcca ccgggctatt tcgaaatcga gggtgcacca
     5641 gggattggcg gtagcaactt gcccaagtcg ccggtcaatg ggagtttcag ttcgctacaa
     5701 ggtggttcga ctactcccgg tccctgctcc tttgcttcgt gctcgtgtaa gggcggcgaa
     5761 tgttggccat cgcatggatt ccccgtggaa tatgggcatg atttactatt cgattgacgc
     5821 aggcggagct gggctagcga catcggaccg tcagaaatgt ttggctgact gggaactgca
     5881 agcacagaac catcctgcct gtgcgtcagt cgcaggtaac gggtggttag ctggatcctc
     5941 gacgtttgga gcagcagcca tatgtgcttt ggtgccagga agtctgggcg tcgcggcgat
     6001 accttgctta gccgcgtttg gaaacgccat cgttcagaat ctggttttca caaactcaaa
     6061 acaggcgtgc aatcaagcgc aggtctatac gggcagctgc gactaacgag tagcaatcag
     6121 tttggggata tctgtcgatg gtttcgttcg tatcgaacaa cgtgatgccc attaccttgg
     6181 ctgcgttctt ggcactactt cttttatcgt tttatttgtc gcgcggcagg cccgaggata
     6241 gaaaacacac ctatcgcggg ttggccgcct gggccatcat aacggcggta tacgttgtaa
     6301 cgagaatatt ctcttaattt tttcaagggc caatgagaga atgttgctgc ccggcggtga
     6361 gattcaacgt gaatatgcga ggtacaacga gacaagaccc ggccagcact actgtccagg
     6421 acgtcaagtc cgaaaaggtc ctgtactgct tcaagggtga cgatgtcgcc gacgcggcga
     6481 aaagcatgcg caagcagcag gtctaccgcc tgactgtgct cgacgagcgc gacaacaaga
     6541 agctgtgcgg cgtgatcccg ctgggcgaca tcctgcgcca caagcagggc gagctggccg
     6601 agaacaccgc cgatgcgatc gtggcatgac gccaggctag cctgcgaggc gcaacgccag
     6661 gcagtaagaa tcaaggggcg cgtcccgtcg ggacgtgccc cttttcgttc gccggcggct
     6721 aatgcgctat ccgcacttcg agtcgccgca gttcaggcag gtcatgcagc cgtccatcat
     6781 caccacggcg gtggtgttgc acttggcgca cagctgcgcg ccctcgggga aggcgctgtc
     6841 cgcgaaggcg tcctgctgcg cggcgcgcgc ctcgaactcg gcgcgcttct gctcgaccag
     6901 ctggcgctgg tgctcgtcga ggcccggcgc ctgcatcatg ccgatgctga ccaggtgctt
     6961 ctcgaccacc tcgccgagct cggcgatcaa ggagggcatg tacttgccgc ccggcttcca
     7021 gtagccgccg cgcgggtcga acaccgcctt gagctcctcg accaggaaag tgacgtcgcc
     7081 gcccttgcgg aacaccgcgg acatgatccg cgtcagcgcc acgatccact ggtagtgatc
     7141 gaggttcttg gagttgatga agatctcgaa gggccggcgc gcctcgtgct tggtcccggc
     7201 gttgagcagg atgtcgttga tggtgacgta catcgcgtgc tcggcgaccg gcggcttgat
     7261 cttgtaggtc gcgccgatga gcatgtcggg acgctcgagc ttctcgtgca tccggatgac
     7321 cttgccctcc gtgccctcct cgacgcgcgg cttgggcggc ggcgccttct cctcgggctt
     7381 ggcgacgtga taagcgacga tcttcttgtc tatcttgtgt gctgacatga tgttttctca
     7441 gaacttgccg taatagcctt ccttgagggc gtcgaacagg ttggcggcgg tgtgcatctc
     7501 gccgtcgtat tcgacttcct cgtccccctt gagctcgatg gtgctgccgt cctcgagctc
     7561 gaacacgtag gtcgtgttct tgaggtcctg ctccttgacc agcacgccct ggaaggccgc
     7621 cgggttgaag cggaaggtgg tgcagccctt gagcccctgc tcgtaggcgt acatgtagat
     7681 gtccttgaag tcctcgtaag gatagtccgt cgggacgttc gcggtcttgg aaatcgacga
     7741 gtcgacccac atctgcgccg ccgcctggat atcgacgtgc tggcgcggcg tgatgtcctc
     7801 ggcggtgatg aagtagtcgg gcagcttctc
//
LOCUS       DAOMPG010000105         7761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4040_length_7761_cov_4.245004, whole genome shotgun sequence.
ACCESSION   DAOMPG010000105 DAOMPG010000000
VERSION     DAOMPG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4040_length_7761_cov_4.245004"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLS34_07135"
     CDS             <1..551
                     /locus_tag="PLS34_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HRP87277.1"
                     /translation="PISECYWVESGRLLAGEHPGHWDESVVRRRIVGLLDAGIRVFID
                     LSARADGVYPYHEQLNKICRDRGAVSEYLWRPLPEFEVPEHPDDVAHILRVIKTAIDS
                     GDRVYLHCNDGVGRTGMVAGCWLVERGFDPEDALDELARRFAAMNKSRHYRCTPSSGL
                     QAEWVENWEPALGLSAERSQAC"
     gene            complement(568..2067)
                     /gene="nhaC"
                     /locus_tag="PLS34_07140"
     CDS             complement(568..2067)
                     /gene="nhaC"
                     /locus_tag="PLS34_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRP87278.1"
                     /translation="MSEPQDSGASIRLPSLLQSLTPIAALIALLSMSIWLFGADSSQG
                     ANQVALTLAAAVAALIGIYNGERWTDILQAIVNGVSTALGAILILFAIGALIGSWSLS
                     GTVPAMIYYGVQILSPTVFYPTACLICALVSVSVGSSWTTAGTIGIGLMGVAQGLGLS
                     PEVTAGAVISGAYFGDKMSPLSETTNLAPAVAGVELFTHIRHMSYTTGPSFLIALVGF
                     TLVGFLGDGPSGELALGELPAVLAANFSLSAWTLLPLMVVLGLALARFHALPVILLGA
                     LLGAVLAVVLQPEVVAAYGGTGELGRGLAMLKGAWISLFDGFVADTGNAVIDDLLSRG
                     GMSSVLTTVWLVISALAFGAVLEHAGMLQRIIQGVIRAARSTGALITAVLCTSIGTNI
                     VTGDQYIAIVLPGRMFRAEFRRRGLRARNLSRTIEDAGTLTSPLIPWNTCGAYMATTL
                     GVATLAYLPWCFLNLVNPLIAALLGFRGITIDRIPADEAGEEIDSHARV"
     gene            complement(2071..3408)
                     /locus_tag="PLS34_07145"
     CDS             complement(2071..3408)
                     /locus_tag="PLS34_07145"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRP87279.1"
                     /translation="MSEKPKPPNGRRRSRVPVTRTGRLFRMGLTAGGMAVGGLAEGLR
                     RATGKASGTAASALLSGANATRLARRLAHMRGAAMKLGQLLSMESSHVLPPEFAEALA
                     VLRASADVMPQTQVKRLMGREYGKGWEQRFAEFDFEPAASASIGQVHRAVASDGRAMA
                     LKIQYPGVAKSIDSDVDNLAMFLNMARLLPRELDVSDIIDEAKRQLRQEADYLTEAAN
                     LERYHELLADDHRFVVPRVHRDYSTQRILAMDWVEGEPLDKLVREQVPQDIRDGIATA
                     IQDLTFMELYEFRFMQTDPNFANYLYRHEDGRLVLLDLGAAVQFPQDFVDRYRRITRA
                     ILDHDRERVARHAAEIGYLREADSPRHKELVVDLIMMVCEPLTTDGAYDFGASNLPSR
                     AAAAGLNIVFRSREYRNPPASTAVLHRKLGGSFLLCQRLGARVEMRDYLLARI"
     gene            complement(3422..4492)
                     /locus_tag="PLS34_07150"
     CDS             complement(3422..4492)
                     /locus_tag="PLS34_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRP87280.1"
                     /translation="MPTNVTPEYKKAEEAYRKAREPRERLDCLRQMLSTIPKHKGTEH
                     LQADIKSRIKDLTEELAGPKKGGKRSGPALTLRPEGAAQIALLGPPNSGKSALHQRLT
                     GSRAQSGPGSFITQFPEPGMLPYEDIAFQLLDLPSMSAEHSPPWIASTLQTADAALLV
                     VDLSDPGCVEQVQFILDTLATRRIRLVPEWPGVPRGDQPGGSAAAAGSADELADPFRI
                     DLPTLLLANKSDLAPNPDEVAVLEELLGVTFPALRVSAETGLGLGEIAPWLFGALRIV
                     RVYTKAPGKDPERDRPFTVRGGDTVLDVARLVHKDIAASFRFGRLWGSGVFDGQQVGA
                     EHPVADGDIVELHAARAASEPA"
     gene            complement(4502..>5221)
                     /locus_tag="PLS34_07155"
     CDS             complement(4502..>5221)
                     /locus_tag="PLS34_07155"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRP87281.1"
                     /translation="LNGSADKAAVLAGWRDMKAAPGQSLGWGLLVLALSWLVSLVALW
                     LGSWVMLLSLLSGFVFIGPLLAIALYAVSWQLEAGRVPTFDRSIGAARTRLGDGMVFA
                     LILLVIFLVWARAGSMVHVFFPVESRPETIQLVRFLAIGSAVGSIFAAITFAASAFSL
                     PMLMDRKVDTVTAVVTSVNATLRNKPAMVVWLALIVLLTGVGFASAFIGLVVLFPLLG
                     HATWHAYRDVVDASAWPAHEA"
     assembly_gap    5222..5231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5467..7692
                     /locus_tag="PLS34_07160"
     CDS             5467..7692
                     /locus_tag="PLS34_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87282.1"
                     /translation="MKNLFAELKRRNVIRIAIFYGAAAWLILQVADVVLDILDAPDGS
                     LRLLAAVLALGFPFALILAWAFEVTPEGIKLESQVDRDRLPSDQSARKLDIATIGLLL
                     LAIVLLLWQQLGQQAGDAPPLAQQAAPADAARADRPRDLSIAVLPFVNMSADRENEYF
                     SDGLSEEILNVLAKMQDFRVAGRTSSFAFKDQQTDLRAIGERLGVANILEGSVRRQGE
                     RIRVTAQLIDARDGYHLWSETYDRRLDDIFAIQDDIATEVVKALRLTLLAADEEVISA
                     TAKGDVEAYNHYLLGQSLVRLRTREGLERALEEFQQAILIDSEFAPPYAGIAMVYALF
                     DNYGYRRLGETDELAHRALERALALDPQSDEAWAVRGLLLNQGPGASRRTAEARAALE
                     RAIEINPNNALAHLWIANLLFPDFEAVGAALRRAYELDPLSPVIVYRQAMNTLQSQDF
                     AAYERYQRELEEIAPDWFITWQSAGAGAQQAGRVAEAALAYERAVELNPEYIGGQAQL
                     GFTLETLDYQARAERMLERALELHDMDDARLMLAALRARKALAEQGHKAAAAIYREAV
                     ESLGPPMADRTAELAMLEIGAGEIDVAERRLRQVLYQDGDGADGVGASTPEYLLPQLA
                     LATALWMKGEPVASRRHAAVVRGIAREFESQGIAVNFMPLVLGALDYMEGKTDALYSG
                     LEQSIREGFRLPPGNLAWMTPFVTDTAETERLERLMGDVLAQERARYDAAKAATPAAS
                     D"
BASE COUNT         1248 a         2661 c         2692 g         1150 t
ORIGIN      
        1 agccaatcag cgaatgctac tgggtcgaat ccggccggct gcttgccggc gagcacccgg
       61 ggcactggga cgagagcgtc gtccgccggc gcatcgtcgg actcctggat gccggaatcc
      121 gcgttttcat cgacctgagt gcgcgcgccg acggcgtcta tccctaccac gaacagctca
      181 acaagatctg ccgcgaccgc ggcgcggtca gcgaatatct ctggcgtccg ctgccggaat
      241 tcgaggttcc ggagcacccg gacgacgtgg cccacatcct gcgggtcatc aagacggcaa
      301 tcgactccgg ggaccgcgtc tacctgcact gcaatgacgg cgtgggccgg accggcatgg
      361 tggccggctg ctggctggtc gagcgcggct tcgacccgga ggacgcgctc gatgagctcg
      421 cgcgccgctt cgccgcgatg aacaagtcgc gacactaccg ctgcacaccc tccagcgggc
      481 tgcaggccga gtgggtcgag aactgggagc cggcgctcgg cttgtccgcg gagcgtagcc
      541 aggcctgctg agcaccctgg tccgcgctta cacgcgggcg tggctgtcga tctcctcgcc
      601 cgcttcatcg gccggtatgc ggtcgatggt gatgccgcgg aaccccagca gtgccgcgat
      661 cagcggattc acgagattga ggaaacacca cggcaggtag gccagcgtgg ctacgccgag
      721 cgttgtcgcc atgtacgcgc cgcaggtgtt ccacggaatc agcggcgagg tcagcgtgcc
      781 ggcgtcctcg atggtgcgcg acaggttgcg cgcccgcagg ccgcggcgac ggaactccgc
      841 gcggaacatc ctgcccggca gcacgatggc gatgtactgg tcgccggtga cgatgttggt
      901 accgatcgag gtgcacagca cggccgtgat cagggccccg gtgctccgcg ccgcccggat
      961 cacgccctgg atgatccgct gcagcatgcc ggcatgctcg agcaccgcgc cgaacgccag
     1021 cgcggagatg accagccaga cggtagtcag cacgctgctc atgccgcctc gcgacagcag
     1081 gtcgtcgatc accgcgttgc cggtgtcggc gacgaagccg tcgaacaggc tgatccaggc
     1141 gcccttcagc atggccaggc cgcggccgag ttcgccggtg ccgccgtagg ccgcaaccac
     1201 ctcgggctgc agcaccacgg cgagcacggc gccgagcagc gcgcccagca ggatcacggg
     1261 cagggcgtgg aagcgggcca gcgccaggcc cagcacaacc atcagcggca gcagcgtcca
     1321 ggcactcagg ctgaagttcg ccgccagcac cgccggcagc tcgccgagcg ccagctcgcc
     1381 ggacgggcca tcgccaagga agccgaccaa agtgaagccg accagggcga tcaggaacga
     1441 cggcccggtc gtgtagctca tgtggcggat atgggtgaac agttcgaccc cggccaccgc
     1501 cggagcaagg ttggtggtct cggacagcgg cgacatcttg tcgccgaaat acgctcccga
     1561 tatcaccgcg ccggccgtca cttcgggcga caggcccagc ccctgcgcga cgcccatcaa
     1621 ccctatgccg atggtgcccg cggtcgtcca gctggagccg acggatacgg acaccagcgc
     1681 acagatcagg caggcggtcg ggtagaacac cgtcggcgac agaatctgta cgccgtagta
     1741 gatcatcgcc ggcacggtac cggacaggct ccagctgccg atcagcgcgc cgatggcgaa
     1801 caggatcagg atggcgccga gcgccgtcga gacgccattg acgatggcct ggaggatgtc
     1861 ggtccagcgc tcgccgttgt agatgccgat cagcgccgcg accgccgccg ccagcgtgag
     1921 ggccacctgg ttggcgccct gcgacgagtc tgcgccgaac agccagatgg acatggagag
     1981 caacgcgatc agcgccgcga tcggcgtcag cgactgcaac aggctcggca ggcgaatgct
     2041 cgctccgctg tcctgcggct cgctcaagcc tcagatcctg gcgagcaggt agtcgcgcat
     2101 ctcgacgcgc gcccccaacc gctggcagag caggaaactg ccgccgagct ttcggtgcag
     2161 cacggcggtc gacgccggcg gattacggta ctcccgcgag cggaacacga tgttcaggcc
     2221 ggccgccgcg gcgcgcgagg gcaggttcga ggcgccgaag tcataggcgc cgtcggtggt
     2281 cagcggttcg cacaccatca tgatcaggtc gacgaccagc tccttgtggc gcggcgagtc
     2341 cgcctcgcgg aggtagccga tctcggccgc gtgcctggcg acgcgctcgc ggtcgtggtc
     2401 gagaatggcg cgcgtgatcc gccggtagcg atcgacgaag tcctgtggaa attgcacggc
     2461 ggcgccgagg tccagcagga cgagacgacc gtcctcatgc cggtacaggt agttggcgaa
     2521 gttcgggtcg gtctgcatga agcggaattc gtacagctcc atgaaggtga ggtcctggat
     2581 ggccgtcgcg atgccgtcgc gtatgtcctg gggaacctgc tcgcgcacca gcttgtcgag
     2641 cggctcgccc tcgacccagt ccatggcgag gatccgctgg gtgctgtagt cgcgatgcac
     2701 ccggggcacg acgaagcgat ggtcgtccgc cagcagttca tggtagcgct cgagattggc
     2761 cgcctcggtc aggtagtcgg cttcctggcg caactgccgc ttcgcctcgt caatgatgtc
     2821 cgacacgtcc agctcgcgcg gcagcaggcg cgccatgttc aggaacatcg cgaggttgtc
     2881 gacgtcgctg tcgatgctct tggcgacgcc cgggtactgg atcttcagcg ccatggcgcg
     2941 gccgtcgctg gcgaccgcgc ggtgcacctg gccgatggac gccgacgccg ccggctcgaa
     3001 gtcgaactcg gcgaagcgct gctcccagcc cttgccgtac tcccggccca tcaggcgttt
     3061 cacctgggtc tgcggcatca cgtcggccga ggcgcgcagc accgcgagcg cttcggcgaa
     3121 ctccggcggg agtacgtggc tgctctccat ggacagcagc tgcccgagct tcatggccgc
     3181 gccgcgcatg tgcgcgaggc gcctggcgag gcgggttgca ttggcgccgg acagcagggc
     3241 cgaggccgcc gtgccggagg ccttgccggt ggcgcgccgc aggccttcgg ccagcccgcc
     3301 gaccgccatg ccgccggcgg tcagccccat gcggaacagc cgtccggtgc gggtgacggg
     3361 gactcgcgac cggcgtcgtc cattcggcgg cttgggtttc tcgctcacga ggctgagcct
     3421 atcacgccgg ctcagatgcc gcgcgtgccg catgcagctc cacgatgtcg ccgtccgcga
     3481 cgggatgctc ggcgccgacc tgctggccgt cgaatacgcc gctgccccac aggcggccga
     3541 agcggaagct cgccgcgatg tccttgtgca ccagccgcgc gacatccagc accgtgtcgc
     3601 cgccgcgcac cgtgaagggc cggtcgcgct cgggatcctt gccgggcgcc ttggtgtaga
     3661 cccgcacgat ccgcagggcg ccgaacaacc agggcgcgat ctcgcccagg cccaggccgg
     3721 tctccgccga cacgcgcagc gccgggaagg tcacgccgag cagctcttcc agcaccgcca
     3781 cttcatcggg gttcggcgcg aggtcgcttt tgttggccag cagcagcgtc ggcaggtcga
     3841 tccggaacgg atcggcaagc tcgtcggcgg acccggccgc cgcggcggag ccgccgggct
     3901 ggtcgccccg gggcacgccg ggccactcgg gcacgaggcg gatcctgcgc gtcgccagcg
     3961 tgtcgagaat gaactgcacc tgctcgacac agccgggatc agacaggtcg accacgagca
     4021 gcgcggcgtc ggccgtctgc agcgtcgagg cgatccacgg cgggctgtgt tcggcggaca
     4081 tcgacggcag gtcgagcagc tggaacgcga tgtcctcgta ggggagcatg ccgggctccg
     4141 ggaactgggt gatgaacgag cccggccccg actgggcgcg cgagccggtg aggcgctggt
     4201 gcagggccga cttgccggag ttgggcgggc cgagcagcgc gatctgcgcc gcgccctccg
     4261 ggcggagcgt cagcgccggc ccgctgcgct tgccgccttt tttcggcccc gccagttcct
     4321 cggtcaggtc cttgatgcgg ctcttgatgt cggcctgcag gtgctctgta cccttgtgct
     4381 tggggatggt ggacagcatc tgcctgaggc aatcgagtcg ctccctgggc tcgcgcgcct
     4441 tgcgataggc ttcctccgcc ttcttgtact cgggggtgac gttggtcggc acggcgtttg
     4501 ctcaggcctc gtgcgcgggc caggcggagg cgtcgaccac gtcgcggtag gcatgccagg
     4561 tcgcgtgtcc cagcagtgga aacagcacca cgagcccgat gaaggccgaa gcgaagccga
     4621 cgcccgtcaa caggacgatc agcgccagcc acaccaccat cgccggcttg ttgcgcaagg
     4681 tcgcgttgac gctggtgacc accgccgtga cggtgtcgac cttgcggtcc atcaacatgg
     4741 gcagcgaaaa ggcgctggcc gcgaaagtga tggcggcgaa aatcgagccc acggcggagc
     4801 cgatggccag gaagcgcacc agctggatcg tctccggccg ggactcgacc gggaagaaca
     4861 cgtgcaccat ggagccggcc cgcgcccaca ccagaaatat caccagcagg atgagtgcga
     4921 acaccatgcc gtccccgagg cgtgtgcgcg ccgccccgat gctgcgatcg aaggtcggta
     4981 cgcggcccgc ctccagctgc cagctcacgg cgtacagggc gatcgccagc aacggcccga
     5041 tgaacacgaa gcccgacagc agcgacagca gcatcaccca gctgccgagc cacagcgcca
     5101 cgaggctcac cagccaggac agcgccagca ccagcaggcc ccaccccagg ctctgtcccg
     5161 gcgcagcctt catgtcgcgc cagccggcca acacggcggc cttgtccgcc gagccgttga
     5221 gnnnnnnnnn nccgggaccc gccggcaggg cgcgacgaac ggcagcgccc cgtccgcttc
     5281 gctttgcccg cctgctgatg gctcgttttc catacccgtc gccgtgctcg aggccaggcc
     5341 atttctatac cacatgccgg cccggccggg tctgcgccgc gccggaggta gtcgctttcg
     5401 catctggccg gcgttatctt gtgcggggcc cgaaccgccg gccgccgacc cgtcgcagcg
     5461 ttgccgatga agaacctttt cgccgagctg aagcgccgca atgtaatccg catcgccatc
     5521 ttctacggcg cagcggcatg gctgatcctg caggtcgcgg acgtcgtgct ggacattctc
     5581 gacgccccgg acggttcgct gcgcctgctg gccgcggtgc tggcgctcgg ttttcccttt
     5641 gccctgattc tcgcctgggc cttcgaggtc acgccggaag gcatcaagct cgaaagccag
     5701 gtggatcgcg accgcttgcc gtccgaccag tcggcacgca agctggacat cgccaccatc
     5761 ggcctgctgt tgctggcgat cgtgctgctg ctttggcaac agctcggcca gcaggcgggc
     5821 gatgcgccgc cgctcgcaca gcaggccgcg cccgcggacg cggcgcgggc cgaccggccg
     5881 cgcgacctgt ccatcgcggt gctgcctttc gtgaacatgt cggcggaccg cgagaacgag
     5941 tatttctcgg acggcctgtc cgaggaaatc ctcaatgtgc tggcgaaaat gcaggacttc
     6001 cgggtggccg ggcggacctc ctcgtttgcg ttcaaggatc agcagaccga cctgcgcgcc
     6061 atcggcgagc ggctcggcgt cgccaacatt ctcgagggca gcgtgcgccg ccagggcgag
     6121 cgcatccggg tcacggcgca gttgatcgac gcgcgcgacg gctaccacct ctggtccgag
     6181 acctacgacc ggcggctgga cgacatcttc gccattcagg acgacatcgc taccgaggtg
     6241 gtgaaagcgt tgcggctgac gctgctggcg gccgacgaag aggtcatcag cgcgaccgcg
     6301 aaaggcgacg tcgaggccta caaccactac ctgctcggcc agtcgctggt ccgcctgcgc
     6361 acccgcgagg ggctggaacg cgcgctggag gaattccagc aggcgatcct catcgactcg
     6421 gagtttgcgc cgccctacgc gggcatcgcg atggtctacg cgctgttcga caactatggc
     6481 taccggcgcc tcggcgagac tgatgaactc gcgcaccggg cgctggagcg cgcgctcgcg
     6541 ctcgacccgc agagcgacga ggcctgggcg gtgcgcggcc tgctgctcaa ccagggcccc
     6601 ggggcgtcgc ggcggaccgc cgaggcgcgt gcggcgctcg agcgcgccat cgagatcaac
     6661 cccaacaatg cgctggcgca cctgtggatc gccaacctgc tgtttccgga cttcgaggcc
     6721 gtcggtgcag ctctgcggcg ggcttacgag ctcgatcccc tctcgccggt gatcgtctac
     6781 cggcaggcga tgaatacctt gcagagccag gatttcgctg cctacgagcg ctaccagcgc
     6841 gagctggagg aaatcgcgcc ggactggttc atcacctggc agtcggccgg cgcgggggcg
     6901 cagcaggccg gccgggtggc cgaggcggca ctggcctacg agcgcgcggt ggaactgaat
     6961 ccggagtaca tcggcggcca ggcgcagctg ggattcacct tggagactct ggactaccag
     7021 gcccgcgcag agcgcatgct cgaacgggcg ctggaattgc acgacatgga cgatgcgcgc
     7081 ctcatgctcg ccgcgctgcg cgcgcgcaag gcgcttgccg aacagggaca caaggctgcg
     7141 gcggccattt accgcgaggc cgtggagtcg ctcggcccac cgatggccga ccgcaccgcc
     7201 gagctcgcca tgctggaaat cggcgccggc gagatcgatg tcgccgagcg caggctgcgc
     7261 caggtcctct accaggacgg cgacggcgca gacggcgttg gcgcaagcac gccggagtac
     7321 ctcctcccgc aactggcgct ggcgacggcc ctgtggatga agggcgagcc tgtcgcctcc
     7381 cggcggcacg ccgcagtcgt caggggcatt gccagggagt tcgagtccca gggcatcgca
     7441 gtgaacttca tgcccctggt gctcggcgcg ctggactaca tggaaggcaa gacggatgcg
     7501 ctctactccg gcctggagca gtccatccgg gaaggcttcc ggttgcctcc aggcaacctg
     7561 gcctggatga cgccgttcgt gaccgacacc gccgagaccg agcggctcga acggctgatg
     7621 ggcgacgtgc tggcacagga gcgggcacgc tacgacgcgg ccaaggcggc cacgccggcc
     7681 gcgtccgact gaggggcggc gccggatcgg cgggcctggg gcgggtcagg cggcgatcgt
     7741 cttgtcgacc aggatgcggt t
//
LOCUS       DAOMPG010000106         7721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4060_length_7721_cov_4.353639, whole genome shotgun sequence.
ACCESSION   DAOMPG010000106 DAOMPG010000000
VERSION     DAOMPG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_7721_cov_4.353639"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLS34_07165"
     CDS             complement(<1..646)
                     /locus_tag="PLS34_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRP87283.1"
                     /translation="MKSFWKTLCIALLAGLAAHAAQAEERIKDLAVVSGVRSNQLVGY
                     GLVVGLSGTGDQTTQAPFTAQSLRNMLGQLGVTIPGNVNLQLNNVAAVAIHAELPPFA
                     KLGQTIDITVSSIGNAKSLRGGSLLMAPLKGADGQVYAIAQGNLVVGGFGAEGRDGSR
                     ISVNVPSAGRIPSGATVERVVPSGFNATDSITLNLRSPDFTTAYRLAEAVNKAIG"
     gene            complement(666..1367)
                     /gene="flgH"
                     /locus_tag="PLS34_07170"
     CDS             complement(666..1367)
                     /gene="flgH"
                     /locus_tag="PLS34_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRP87284.1"
                     /translation="MASPGSRNFTIAALLAALLSAGCATRPAPIERGDDRAYQAVEAQ
                     QPVPPEANHGAIFQAANASALFEDFKARRVGDILTVLLAERTNARKSANANTSKDSSL
                     SLLDPTLLGRPVTRDGVPLLNNELDMSRSFDGKGDAAQSNQLEGSITVTVAAVMPNGN
                     LVIQGEKWVKINQGQEYIRLRGIVRPVDIRSDNTVLSTQVGDAQVAYGGTGTVAHASS
                     PGWLTRFFNSPIWPF"
     gene            complement(1370..2161)
                     /gene="flgG"
                     /locus_tag="PLS34_07175"
     CDS             complement(1370..2161)
                     /gene="flgG"
                     /locus_tag="PLS34_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRP87285.1"
                     /translation="MSQILWIAKTGLDAQQTRMAVISNNLANVNTTGFKKGRAVFEDL
                     IYQNVRQAGAQSSQDTQLPSGLMLGTGVRTVATEKSFTQGNLVQTDNALDVAVEGRGF
                     LQILMPDGTVGYTRDGSLKIDSEGRFVTASGYELQPGIVLPQNTQSVTISRDGIVTAT
                     VPGSVAPVQVGTLQLADFINPAGLQPAGENLFLETAASGPPQVGNPGLNGLGTLMQGS
                     LEGSNVNTVEELVNMIETQRAYEINSKAISTADQMLQYVNNQLGR"
     gene            complement(2192..2932)
                     /gene="flgF"
                     /locus_tag="PLS34_07180"
     CDS             complement(2192..2932)
                     /gene="flgF"
                     /locus_tag="PLS34_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRP87286.1"
                     /translation="MDRMIYLAMTGARETMLAQAGVSHNLANANTTGFKASLQHAQTL
                     EVQGPGHPARAFSMGLDRLADLTPGALQSTGRDLDVAIEGEGWIAVQAPDGGEAYTRA
                     GDLRVDPLGRLTNGAGHPVMGEGGPIALPPFEQVEIGGDGTVTIRPLGQPANALAVVE
                     RIRLVNPDPATLRRGEDGLVRAEPGAAPPADAGVRLRTGALEASNVNTVAALVDMIQL
                     SRQFELQVKMMSTAEDADRASEQLLRMS"
     gene            complement(2947..4164)
                     /gene="flgE"
                     /locus_tag="PLS34_07185"
     CDS             complement(2947..4164)
                     /gene="flgE"
                     /locus_tag="PLS34_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRP87287.1"
                     /translation="MPFRTALSGLNAASADLRVTGNNVANSATVGFKQSRAEFADVFA
                     LSYSGISQNAVGAGVRLSSVTQQFNQGNVDFTGNNLDLALNGQGFFVLSDSGSQVYSR
                     AGAFQVDRNGYVVNSAGQRLQAYPPSNEAGTAFSTGMLSDLQLEAAESPPQATSLVSA
                     TLNLSSQAEDLSALPFDPSDPNTFNYTTSLTTYDSLGQAHTGTMYFRNIGPLQWEAHF
                     AVDGTPVGGAQPLSFNSDGSLAAPLTAVGFGAHDPGNGAAPMNIEINFSQATQFGGTS
                     SVSALRQDGYSTGRLSSIDIDPSGVVLARFTNGQSRALGQVALANFPNPQGLQQLGNN
                     AWAGTFGAGEVVLGAAGTGNFGAIQSGGLEGSNVDLAEQLVNLITAQRNFQANAQVIS
                     TADTVTQTIINLR"
     gene            complement(4222..4893)
                     /locus_tag="PLS34_07190"
     CDS             complement(4222..4893)
                     /locus_tag="PLS34_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRP87288.1"
                     /translation="MAGIDATTLEQAGLSRAPANDARSPDKLGQEQFLKLMITQLSNQ
                     DPFKPLESGEFLGQLAQFGTVSGIQDLQQSFQGLAGSMQSNQALQAAGLVGRSVYVPA
                     QEAWLPPDGAVSGAVQNPGGATNITVGIYDFAGRLVATVPAEGQGDRAAFQWDGTDHD
                     GEQVPPGFYEIRAVGQVSGEPVALDALVRGQVESVSIDRYSGALSLTVTGLGVVDLNQ
                     VRQIG"
     gene            complement(4896..5321)
                     /gene="flgC"
                     /locus_tag="PLS34_07195"
     CDS             complement(4896..5321)
                     /gene="flgC"
                     /locus_tag="PLS34_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRP87289.1"
                     /translation="MDLSKIFEISGSALSAQSTRLNTVASNLANADAATGNPASVYRA
                     RHPVFATVLEQAAGAGQGQPAAGVRIAGIVESQAPPIARYEPGHPLADEQGYIYLPAI
                     NTVEQMADMISASRSYSSSVEVLSTTKELMLRTLQLGRS"
     gene            complement(5325..5726)
                     /gene="flgB"
                     /locus_tag="PLS34_07200"
     CDS             complement(5325..5726)
                     /gene="flgB"
                     /locus_tag="PLS34_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRP87290.1"
                     /translation="MSSIVDRALGVHTQALEVASRRLELLASNIANADTPNYKARDID
                     FRTALTRAADERGAAAMAVTQPGHIRSTAGAGTPGEPQYRVPNHAALDGNTVDGQLEK
                     AAFAENAVRYQASLDFINGRLRSLRTALKGE"
     assembly_gap    6153..6196
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6197..6780
                     /gene="flgA"
                     /locus_tag="PLS34_07205"
     CDS             <6197..6780
                     /gene="flgA"
                     /locus_tag="PLS34_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRP87291.1"
                     /translation="AVKVEISIGRLDGRLRLPACTLPLETELPPGARPLGAVAVGVRC
                     TAPGWSLFVPARVAVSRPVVVLAASRPRGATLSAADLALEPRDLSTLPGGFLTDPDEL
                     VGMTLRRPVQARTVLLPGHAAAPELVRRGERVILQAAGGTIAVQVEGEALAGGALGER
                     VRVRNLATRRVVEGTVGGEGLVVMGGTGPGRLN"
     gene            6863..7171
                     /gene="flgM"
                     /locus_tag="PLS34_07210"
     CDS             6863..7171
                     /gene="flgM"
                     /locus_tag="PLS34_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRP87292.1"
                     /translation="MSNEIKSLHPMEPRRNGAVQGAAPAGKQVEAGREAAARGPAEDK
                     VTLTDAARTITSMSAELAREGTFDGAKVTQLRAAIESGRYQADPVAIADALMRFDQQG
                     "
     gene            7202..7630
                     /locus_tag="PLS34_07215"
     CDS             7202..7630
                     /locus_tag="PLS34_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRP87293.1"
                     /translation="MSEALKIVGNRRQDSPPLQRCAEVFDALEETLLEEQQALLDRDP
                     EVLLVVAERKRGLLVELQRIAASLVELPDAAPALRERLEATFARCRQLNQINGGVVAA
                     SRGATEKALALLRGDQVSVSLYDSHGDTRATGGSRSLAQA"
BASE COUNT         1069 a         2543 c         2810 g         1255 t
ORIGIN      
        1 cgccgatcgc cttgttgacc gcctccgcaa gccggtacgc ggtggtgaaa tcggggctgc
       61 gcaggttgag cgtaatgctg tcggtggcgt tgaagccgga gggcaccacg cgctcgacgg
      121 tggcgccgct ggggatccgc cccgcgctcg gcacgtttac ggaaatgcgc gagccgtcgc
      181 ggccctcggc gccgaagccg cccaccacca ggttgccctg ggcgatggcg tacacctggc
      241 cgtcggcgcc cttcagcggc gccatcagca ggctgccgcc gcgcaggctc ttggcgttac
      301 cgatcgagga caccgtgatg tcgatggtct ggccgagctt ggcgaaaggc ggcagctcgg
      361 catggatcgc caccgccgcc acgttgttga gctgcaggtt gacgttgccg gggatggtga
      421 cgcccagctg gccgagcatg ttgcgcaggc tctgcgcggt gaacggcgcc tgcgtggtct
      481 ggtcgccggt gccgctcagg cccaccacca ggccgtagcc caccagctgg ttgctgcgca
      541 cgcctgagac cacggcgagg tccttgatgc gttcctcggc ctgcgccgcg tgcgcggcca
      601 gcccggccag cagcgcgatg cacagggtct tccagaagct tttcattgcg ggcactccaa
      661 tcgcctcaga acggccagat ggggctgttg aagaagcgcg tcagccagcc cggcgagctg
      721 gcgtgcgcca ccgtgccggt gccgccgtag gcgacctggg catcgccgac ctgggtggac
      781 agcaccgtgt tgtcggagcg gatgtccacc gggcggacga tgccgcgcag gcggatgtat
      841 tcctggccct ggttgatctt cacccacttc tcgccctgga tgaccaggtt gccgttgggc
      901 atcaccgcgg ccacggtgac ggtgatcgag ccctcgagct ggttgctctg cgccgcgtcg
      961 cccttgccgt cgaaggagcg gctcatgtcg agctcgttgt tgagcagcgg cacgccgtcg
     1021 cgcgtcaccg gccggccgag cagcgtcggg tccagcaggc tgaggctgct gtccttggag
     1081 gtgttggcgt tggccgactt gcgcgcgttg gtgcgctcgg ccaaaagcac cgtgaggatg
     1141 tcgccgaccc ggcgggcctt gaagtcctcg aacagggccg aggcgttggc cgcctggaag
     1201 atggcgccgt ggttggcctc gggcggcacc ggctgctgcg cttccactgc ctggtaggcg
     1261 cggtcgtcgc cgcgctcgat cggtgcgggc cgggtggcgc agccggccga caggagcgcg
     1321 gccagcagtg cggcgatggt gaaattgcgg gatccggggc tggccatggc tatcgtccga
     1381 gctggttgtt cacgtactgc agcatctggt cggcggtgga gatcgccttg gagttgatct
     1441 cgtaggcgcg ctgcgtctcg atcatgttca cgagctcctc cacggtgttg acgttggagc
     1501 cctcgagcga gccctgcatc agcgtgccga ggccgttcag gcccgggttg ccgacctgcg
     1561 gcgggccgct cgcggcggtc tccaggaaga ggttctcgcc agccggctgc aggccggccg
     1621 ggttgatgaa atcggcgagc tgcagggtgc cgacctgcac cggtgcaacg ctgcccggca
     1681 ctgtcgcggt gacgatgccg tcgcggctga tggtgacgct ctgggtgttc tgcggcagca
     1741 cgatgccggg ctgcagctca tagccgctgg ccgtgacgaa gcgaccctcg ctgtcgatct
     1801 tcagcgaacc gtcgcgggtg taacccaccg tgccgtccgg catgaggatc tgcaggaagc
     1861 cgcggccttc gacggcgaca tccagcgcgt tgtcggtctg caccaggttg ccctgcgtga
     1921 aggacttctc ggtggcgacc gtgcgcacgc cggtgccgag catcaaaccc gagggcagct
     1981 gcgtgtcctg cgaggactgc gcgccggcct ggcggacgtt ctggtagatg aggtcctcga
     2041 acaccgcgcg gcctttcttg aagccggtgg tgttgacgtt cgcgaggttg ttggaaatga
     2101 ccgccatgcg cgtctgctgc gcatccagcc cggtcttcgc aatccacagt atctggctca
     2161 tggcctgtct ccttcagaat cgggcggggc gtcagctcat ccgcaacagt tgctccgagg
     2221 cgcggtcggc gtcctcggcg gtgctcatca tcttcacttg cagctcgaac tgacgggaca
     2281 gctggatcat gtccaccagc gccgccacgg tattcacgtt gctcgcctcc agcgcgccgg
     2341 tgcgcaaccg caccccggcg tcggccggcg gcgcggcgcc cggctccgca cggaccaggc
     2401 cgtcttcgcc acgccgcagc gtggccgggt cggggttcac cagccggatg cgctccacca
     2461 cggccaacgc gttcgccggc tggccgagcg gccggatggt caccgtgccg tcgccgccga
     2521 tctcgacctg ctcgaaaggc ggcagcgcga tcggcccacc ctcgcccatc accgggtggc
     2581 cggcgccatt ggtgagccgg cccagcgggt cgacccggag atctccggcg cgcgtgtagg
     2641 cctcgccgcc gtccggtgcc tgcaccgcga tccagccctc gccctcgatt gccacgtcca
     2701 ggtcgcggcc ggtggactgc aacgcgcccg gggtcagatc ggcgagccgg tcgagcccca
     2761 tcgagaaagc gcgcgccggg tggcccggcc cctgtacctc gagcgtctgg gcgtgctgca
     2821 ggctcgcctt gaagccggtg gtgttggcgt tggcgaggtt gtggctgaca ccggcctgcg
     2881 cgagcatcgt ctcgcgcgcg ccggtcatcg ccaggtagat catccggtcc atttatccct
     2941 gcctgcctag cggaggttga tgatggtctg ggtgacggtg tcggccgtgc tgatgacctg
     3001 cgcgttcgcc tggaaattgc gctgcgcggt gatcaggttg accagctgct cggccaggtc
     3061 gacgttggag ccctcgaggc cgcccgactg gatggcgccg aagttgccgg tgccggccgc
     3121 gccgagcacg acctcgccgg cgccgaaggt gccggcccag gcgttgttgc cgagctgttg
     3181 caggccctgc gggttcggga agttggcgag cgcgacctgc ccgagagcgc gcgactggcc
     3241 gttggtgaag cgggccagca cgacgccgga cgggtcgatg tcgatactgg acagccggcc
     3301 ggtgctgtag ccgtcctggc gcagggcgct gacgctgctg gtgccgccga actgcgtcgc
     3361 ctggctgaag ttgatctcga tgttcatcgg ggccgccccg ttgccgggat cgtgtgcgcc
     3421 gaaaccgacc gcagtcagcg gtgccgccag cgatccgtcg gagttgaagc tcagcggctg
     3481 cgcgccgccg accggcgtgc cgtcgaccgc gaaatgcgcc tcccactgca gcggcccgat
     3541 gttgcggaag tacatcgtcc cggtgtgcgc ctggccgagc gaatcgtagg tcgtcagcga
     3601 agtcgtgtag ttgaaggtgt tcggatcgct gggatcgaag ggcagcgccg acaggtcctc
     3661 ggcctgtgag ctgagattga gcgtcgcgct gaccagcgag gtggcctgcg gcggagactc
     3721 cgcggcttcc agctgcaggt cgctgagcat gccggtgctg aaggccgtgc cggcctcgtt
     3781 cgagggcggg taggcctgca accgctggcc ggccgagttg actacgtacc cgttgcggtc
     3841 cacctggaag gcgccggcgc ggctgtacac ctggctgccg gagtcgctca ggacgaagaa
     3901 gccctggccg ttcagcgcga ggtccaggtt gttgccggtg aagtcgacgt tgccctggtt
     3961 gaactgctgg gtgaccgagg acaggcgcac gcccgcaccg acggcgttct gcgaaatgcc
     4021 gctgtaggac agcgcgaaga cgtcggcgaa ctcggcgcgc gactgcttga agccgaccgt
     4081 ggcggagttg gcgacgttgt tgcccgtgac gcgaaggtcg gcggaggcgg cgttcaagcc
     4141 gctcaaggcg gttctgaaag gcatattcct gttctcctgt tggcggccgt cgtgacggcc
     4201 cgcttgctgg tacgcggcct gctagccgat ctggcggacc tggttgaggt ccacgacgcc
     4261 gagcccggtc accgtcagcg acagggcgcc tgaatatcga tcgatgctga cgctctccac
     4321 ctgcccgcgg acgagggcgt cgagcgccac cggttcgccg gacacctggc cgaccgcacg
     4381 gatttcgtag aagcccggcg ggacctgctc accgtcgtgg tcggtgccgt cccactggaa
     4441 cgcggcacga tcgccctgcc cctcggccgg cacggtcgcc accagtcgcc cggcaaagtc
     4501 gtagatgccg accgtgatgt tggtggcgcc gcccgggttc tgcaccgcgc cgctgaccgc
     4561 gccgtccggc ggcagccacg cttcctgggc cggcacgtac accgagcggc ccaccaggcc
     4621 cgcggcctgc agcgcctggt tggactgcat cgagcctgcc aggccctgga aggactgctg
     4681 caggtcctgg atgccggaga ccgtgccgaa ctgcgccagc tggccgagga actcgccgct
     4741 ctccagcggc ttgaacgggt cctggttgct cagctgggtg atcatgagct tcaggaactg
     4801 ctcctggccg agcttgtcgg gcgacctggc gtcgttcgcc ggcgcccggc tgaggccggc
     4861 ctgctcgagg gtggttgcgt ctattcctgc catgttcagc tccgaccgag ctgcagggtg
     4921 cgcagcatca gttccttggt ggtggagagc acctcgacgc tgctggaata gctgcgcgag
     4981 gcggaaatca tgtcggccat ctgctcgacg gtgttgatcg ccggcaggta gatgtagccc
     5041 tgctcgtccg cgagcgggtg acccggctcg tagcgcgcga tcggcggcgc ctggctctcg
     5101 acgatgccgg cgatgcgcac gccggcggcc ggctggccct ggcccgcgcc ggcggcctgc
     5161 tccagcaccg tggcgaacac ggggtggcgg gcgcggtaca cgcttgcggg gttgccggtc
     5221 gcggcatcgg cgttggcgag gttgctggcg acggtattga ggcgcgtgga ttgggcgctc
     5281 agcgccgaac ccgaaatctc gaagatcttg ctcaggtcca cgggttactc tcccttcagc
     5341 gcggtgcgca gactgcgcag ccggccgttg atgaaatcga gcgaggcctg gtagcgcacc
     5401 gcgttctcgg cgaaagcggc cttctccagc tgcccgtcca cggtgttgcc gtccagcgcg
     5461 gcgtggttcg gcacgcggta ttgcggctcg cccggcgtgc cggcgcccgc cgtgctccgg
     5521 atgtggcccg gctgcgtgac cgccatggcg gcggccccgc gctcgtccgc cgcccgggtc
     5581 agcgccgtgc ggaaatcgat gtcgcgcgcc ttgtaattcg gggtgtcggc gttggcgatg
     5641 ttcgacgcca ggagctccag gcgccgcgac gcgacctcga gggcctgcgt gtggacgcca
     5701 agtgcgcggt cgacgattga actcatgctc gctgtcctcg atgcctgccc gccgtcggac
     5761 gggctcgggg gaaagcaagc aaacggcgtg ccaaccagcc gaaaagtgcg caaaccctgg
     5821 ctatcgacac gccggcacgc cctcgccgac ggttttttga cgccacgggc atggcgcggg
     5881 aagcccgtcc ggcggcgtct gcttgccgcg cgggcggcaa gtccttgccg gccggggtcc
     5941 ggcgcaggcc tggagccggc cggagccggg attggtacgg accttgcagg ctgctatcgc
     6001 agcaactgtg cgaaggatgc gggaaactga tgacaatgcg gacactattg cttgggtgcg
     6061 cgctggcgat cggcggctgg gcggcgttcc cggcgacggc cgcgggcagc ggccaggcca
     6121 tccagccgct cgacaccatc gtcgctgcag ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnaggc ggtcaaggtc gagatcagca tcggccggct cgacgggcgc
     6241 ctgcgcctgc cggcctgcac gctgccgctg gagaccgaac tgccgcccgg cgcgcggccg
     6301 ctgggggcgg tggcggtcgg cgtgcgctgc acggcgcccg gctggtcgct gttcgtgccg
     6361 gcgcgggtcg ccgtcagccg gccggtggtg gtgctggcgg catcgaggcc gcgcggcgcg
     6421 acgctgtccg cagcggacct ggcgctcgaa ccgcgcgacc tgtcgacgct gcccggcggt
     6481 tttctcacgg acccggatga actggtcggc atgacgctgc gcaggccggt ccaggcgcgc
     6541 acggtcctgc tgcccggcca cgcggcggcg ccggagctgg tgcgtcgggg cgagcgggtc
     6601 atcctgcagg cggccggcgg caccatcgcc gtgcaggtgg aaggcgaggc cctggccggc
     6661 ggcgcgctcg gcgagcgcgt gcgagtccgc aatctcgcca ctcgccgggt ggtcgaaggc
     6721 accgtcggcg gcgaggggct ggtggtcatg ggaggcaccg gcccgggccg gctgaactga
     6781 ggcgtgacgc tcctaaagtt ctcgtcacaa acgccgataa gatgctcgtg gaggcgtttg
     6841 aagcgagttc ggggccgtag caatgagtaa tgaaatcaaa agcctgcatc cgatggaacc
     6901 gcgccggaac ggggcggtcc agggtgctgc gcccgcgggc aagcaggtgg aggccggtcg
     6961 cgaggcggcg gcccgggggc cggcggagga caaggtgacg ctgacggatg cggcgcggac
     7021 catcacgagc atgtcggccg aactcgcaag ggaaggcacc tttgacgggg cgaaggtcac
     7081 ccaactgcgc gcggccatcg aatcgggacg ctaccaggcc gacccggtgg cgatcgcgga
     7141 cgcactgatg cgcttcgatc agcagggctg agcagcgcgc ccgcactcca cggagaatcg
     7201 catgagcgaa gcactgaaaa tcgtcggcaa ccgcaggcag gactccccgc cgctgcagcg
     7261 ttgcgccgaa gtgttcgatg ccctcgagga gaccttgctg gaggaacagc aggcgctgct
     7321 cgaccgggac cccgaggtgc tgctcgtagt ggccgagaga aagcgcgggc tgctggtgga
     7381 attgcagagg atcgcagcct cgctggtcga gctgccggac gccgctcccg ccttgcgcga
     7441 gcgcctggaa gcgaccttcg cgcggtgccg ccagctcaac cagatcaacg gcggcgtcgt
     7501 ggctgccagc cgcggggcca ccgagaaggc gctcgcgctg ctgcgcggtg accaggtcag
     7561 cgtgtccctg tacgacagcc atggtgacac gcgcgccacg ggagggtccc ggtccctcgc
     7621 gcaggcctga tcccgaggcc ccgccatggc ctcgcacgag gaactcgtcc gccagctcct
     7681 cgacgatgac gatgtggaga cggcggagac cgtgctcgac c
//
LOCUS       DAOMPG010000107         7666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4111_length_7666_cov_4.779135, whole genome shotgun sequence.
ACCESSION   DAOMPG010000107 DAOMPG010000000
VERSION     DAOMPG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_7666_cov_4.779135"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2941)
                     /locus_tag="PLS34_07220"
     CDS             complement(<1..2941)
                     /locus_tag="PLS34_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HRP87294.1"
                     /translation="MKTAVQQEQAVVVESVNDIPFQAASVDIWEKKYRLVAKDGAVLD
                     RTMDDTYKRIARALADIEPEDRREHWFERFVWALRHGAIPAGRIVSNAGALAHKPATS
                     TINCTVSGTIRDSMDDILQKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGP
                     LSFMDIYDKMCFTVSSAGGRRGAQMGTFDIGHPDVIEFIRAKREDGRLRQFNLSLLVT
                     DEFMQAVANDQPWRLAFPISLKELKGEGLDLENPENIVWREWPTDGDYVRNDNGLVAC
                     RVYKTVPARRVWDVIMTSTYDFAEPGFVLIDRVNEMNNNWWCENIRATNPCVTADTWV
                     QTGDGPRQVADLLGRPFAARVDGADHASGAAGFFRTATKAVLRLQTVEGYALRLTADH
                     RVRRVTRQTRWSLESEWCEAGKLAAGDRVLLNDHREGATWSGARNFDEGYLLGLLVGD
                     GTLKKDAAVLSTWSMPAAVNASAETPRGLMTEALRCARGLPHRADFVGWTEVEGRGEH
                     RLKLAALRDLAFELGMRPGDKAITPAMEKTSSEFHRGFLRGLFDADGSVQGSQEKGVS
                     IRLAQSDLGRLEAAQRMLHRLGIVSRLYRDRRAAGTALLPDGKGGQREYPTLAQHELV
                     ISGDNLAVYLEQVGFSDEAKQARLKSLLSGYRRRLNRERFTATVESVEADGVEDVFDV
                     QVPGINTFDGNGLHAHNCGEQPLPPYGSCLLGSVNLTRFIVDAFTPEAKFDWDKFREV
                     VKVFTRLLDNVVEINGLPLGPQRDEIYRKRRHGMGFLGLGSTITMLGMKYGSKASVAF
                     TQDISRELAMAGWEAALELAREKGPAPIMDEEFTVTEEMLRKRPEMKADGWRAGARIK
                     GRVLHARYSRYMQRVADVNPELVDELAEVGARFTHHSSIAPTGTISLSLANNASNGIE
                     PSFAHHYFRNVIREGRKTKEKVDVFSFELLAYRSLVNAHAMPGSDHPEEKLPDYFITA
                     EDITPRQH"
     gene            3386..3826
                     /locus_tag="PLS34_07225"
     CDS             3386..3826
                     /locus_tag="PLS34_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRP87295.1"
                     /translation="MSLIRYQPWGLMRSLHEDLDRLFEQRLGMDETTGSVANWTPPVD
                     IREEENRFLLHADIPGVKPEDIEVTMENGVLTISGSRAETREQDANGYRRVERVAGRF
                     FRRFTLPDTANAEGITAKSTNGVLEVIIPKHERVMPRRISVDVN"
     gene            4007..5377
                     /locus_tag="PLS34_07230"
     CDS             4007..5377
                     /locus_tag="PLS34_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRP87296.1"
                     /translation="MTARPLAALAAVLSLAVTAPLYAQLPAAVDGAPLPSLAPMVKKT
                     APAVVNIATRGSIEVQGGNPFLDDPFFRRFFDMPDQPRQRRQTQSAGSGVIVNAAEGL
                     VLTNAHVVEGAEEISVTLQDDRTFRAEVVGADRASDVALLRIPAENLVELKMADSGRI
                     EVGDFVVAIGNPFGLGHTVTSGIVSALGRSGINPEGYEDFIQTDASINPGNSGGALVN
                     LRGELVGVNSAILSRGGGNIGIGFAIPSNMARSVMEQLLEFGEVRRGLLGVTILTITP
                     DIAEAYDIKESQGALVSQVMPESAAERAGVKVGDVIRSVDGKSISNANDLRNTIGLKR
                     SGDTVELEIIRDKRKRTVKARLGEQATQAQVSAQDIHPGLQGAELETLDASSPRYEGE
                     AGVLVSNVEPGSPAAQRGLRPGDVIIGVNRRKVSNLAELQQAAQGQALLLNIRRGNAN
                     LILPIR"
     gene            complement(5461..6279)
                     /locus_tag="PLS34_07235"
     CDS             complement(5461..6279)
                     /locus_tag="PLS34_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRP87297.1"
                     /translation="MPTLPALLLIFPAIGALSGLLAGLFGIGGGLVIVPALNAVFAAG
                     LVAMEPAVRMHVAIGSSLAVIVFTAISSVRAHHGYGAVLWPAVRGLAPGIVLGGLLGA
                     WLADLLATRSLEVVFGLFAILMALQIGFGGRVEPQPDAPLPRLPGYLGAGTVIGSLSA
                     LGGIGGGIMTVPYLAWHSVPLRVAVGTAAACTLPVALSGALGYALAGQGAPVPGWATG
                     YLYWPAIGVIALAAMTTAPLGARLAHRLPVKQLRRLFAVLLVVVAADMLIGGRG"
     gene            6370..>7666
                     /locus_tag="PLS34_07240"
     CDS             6370..>7666
                     /locus_tag="PLS34_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87298.1"
                     /translation="MPRIALLTTLVLLLTAAPAHATSRESQREAFRAALPQAEAGRWD
                     RVEPLLPQLAGYALLPDLRAAWLRSQLGRVNDAEVREFLARHPDLAFSAPLRRQWAGS
                     LARRGAWREYLELFKAHYADSHEAVLECHSFTARIRLDRTAGLEEAVLRRWLWPTSQV
                     KECDPAFAWLEQQGAITPERRRARMELALGAGEFRLARWLARPLGAAAVNEVDTWERL
                     HADPAGRLGSPAGWKDSPRARAMLLYGFHRLASSDPEAAARRWPAFRDHFSFDADERA
                     RIDRRIALMHAWRHLPGAYQMLIALPEASQDDSTRTWAVRVAIRAQDWSAIEAAIARL
                     DPVTAQEPAWRYWQARTLEATGRSDAARPIYAELAGERGYYSFMSADRLNADYNWQHT
                     QTPPDERLISALEERADIVRARELFMTGQDSHGRTEWQQA"
BASE COUNT         1226 a         2685 c         2598 g         1157 t
ORIGIN      
        1 cgtgctggcg cggcgtgatg tcctcggcgg tgatgaagta gtcgggcagc ttctcctcgg
       61 ggtgatccga gccgggcatg gcgtgcgcgt tgaccagcga gcggtaggcc agcagctcga
      121 aggagaacac gtcgaccttt tccttggtct tgcgcccctc gcggatcacg ttgcggaaat
      181 agtggtgcgc gaaggacggc tcgatgccgt tggaggcgtt gttggccagc gacagcgaga
      241 tggtgccggt cggggcgatc gagctgtggt gcgtgaagcg cgcacccacc tcggccagct
      301 cgtcgaccag ctccgggttc acgtcggcga cgcgctgcat gtagcggctg tagcgggcat
      361 gcagcacgcg gcccttgatg cgcgcgccgg cgcgccagcc gtccgccttc atctccgggc
      421 gcttgcgcag catttcctcg gtgacggtga actcctcgtc catgatcggc gccggaccct
      481 tctcgcgggc cagctccagc gcggcttccc agccggccat cgccagttcg cgggaaatgt
      541 cctgggtgaa cgcgaccgag gccttgctgc cgtacttcat gccgagcatg gtgatggtcg
      601 agccgaggcc caaaaagccc atgccgtggc ggcgcttgcg gtagatctcg tcgcgctgcg
      661 gtccgagcgg caggccgttg atctcgacga cattgtcgag caggcgcgtg aacaccttga
      721 cgacctcgcg gaacttgtcc cagtcgaact tcgcctcggg cgtgaacgcg tcgacgatga
      781 agcgcgtcag gttgaccgag ccgagcaggc aggagccgta gggcggcagc ggctgttcgc
      841 cgcaattgtg ggcgtgcagg ccgttgccgt cgaaagtatt gatgccgggg acctggacgt
      901 cgaaaacatc ctccacgcca tccgcctcga cgctctcgac ggtggcggtg aagcgctccc
      961 ggttgaggcg gcgacgatac ccggacagca ggctcttgag ccgcgcctgc ttggcttcgt
     1021 ccgagaagcc cacttgctcg agatacacgg cgaggttgtc gccgctgatg accagctcgt
     1081 gctgggccag cgtcgggtac tcgcgctggc cgcccttgcc gtcgggcaac agtgccgtgc
     1141 cggccgcacg ccggtcacgg tacaagcgcg acacgatgcc cagccggtgc agcatgcgct
     1201 gcgcagcttc gagacgcccg aggtcggact gcgccaggcg gatactgacg cccttctcct
     1261 ggctcccctg caccgagccg tccgcatcga acaggccgcg caggaatccg cggtgaaatt
     1321 cgctggaggt cttttccatc gccggcgtga tggccttgtc gcccggccgc atgccgagct
     1381 cgaaggcgag atcccgtaac gcggccagct tgagccggtg ctctccacgt ccttccacct
     1441 cggtccagcc gacgaaatcg gcgcggtgcg gcaagcctcg cgcgcagcgc aacgcctccg
     1501 tcatcaatcc ccggggcgtt tcggccgacg cgttcaccgc cgccggcatc gaccaggtgg
     1561 acaggacggc cgcatctttc ttcaaggtgc cgtcgcccac cagcaggccg agcaggtatc
     1621 cctcgtcgaa gttgcgtgcg cccgaccagg tcgcgccctc gcgatgatcg ttgagcagca
     1681 cccggtcgcc ggctgccagc ttgccggcct cgcaccactc ggattccagc gaccagcggg
     1741 tctggcgggt cacccggcgc acgcgatggt cggccgtaag gcgcaaggcg tagccctcga
     1801 cggtctgcaa gcgcagcacc gccttggtgg cggtgcggaa gaatcccgcg gcgccgctgg
     1861 catggtccgc accgtccacg cgcgcggcga agggccggcc aagcaggtcc gccacctggc
     1921 gggggccgtc gccggtctgc acccaggtgt cggcggtgac gcagggattg gtcgcgcgga
     1981 tgttctcgca ccaccagttg ttgttcatct cgttgacgcg gtcgatgagc acgaagccgg
     2041 gctcggcgaa gtcgtaggtg gaggtcatga tgacgtccca cacgcggcgc gccggcacgg
     2101 tcttgtagac gcggcaggcc accagcccgt tgtcgttgcg cacgtagtcg ccgtcggtcg
     2161 gccactcgcg ccagacgatg ttctcgggat tctcgagatc cagcccctcg cccttcagct
     2221 ccttgaggct gatggggaag gccagccgcc agggctggtc gttggccacc gcctgcatga
     2281 actcgtcggt gaccagcagc gacaggttga actggcgcag ccggccgtcc tcgcgcttgg
     2341 cgcggatgaa ctcgatgacg tcggggtggc cgatgtcgaa agtgcccatc tgggcgccgc
     2401 gccggccgcc ggccgaggac accgtgaagc acatcttgtc gtagatatcc atgaaggaca
     2461 gcgggcccga ggtgtaggcg ccggcgcccg acacgtaggc gccgcgcggc cgcagcgtgg
     2521 agaactcgta gccgatgccg cagcccgcct tcagcgtcag gccggcctcg tggaccttct
     2581 gcaggatgtc gtccatcgag tcgcggatgg tgccggacac ggtgcagttg atggtcgagg
     2641 tggccggctt gtgcgccagc gcgcccgcgt tggagacgat gcggccggcc ggaatggcgc
     2701 cgtggcgcag cgcccagacg aagcgctcga accagtgctc gcgacgatcc tcgggctcga
     2761 tgtcggcgag cgcgcgggcg atgcgcttgt aggtgtcgtc catcgtgcgg tcgagcaccg
     2821 cgccgtcctt cgcgaccagc cggtacttct tctcccagat gtccacggac gccgcctgga
     2881 aggggatgtc attcaccgac tccaccacga ccgcctgttc ttgttgcact gctgttttca
     2941 ttatgcctcg ttccccccgg gcgccccatg gtcctgaaat ttgctggtcc ctctgcaaac
     3001 cgtccaccgt ccggctgccg gctgctcgtg gcggccttcc cggcccccgg cgagcgaaca
     3061 caatatgttg tgttacgggg tggttaaggt aagtgccgga ccgacccctg tcaagcgctg
     3121 atttacgggg gggcgcaagg gtatttacgt cgaaaaaaca ttagtttatg aaatctttat
     3181 agaaattttc tatgaaccgg aggcggctgc cagaaaaaaa ctttctcccg cagcgcaaca
     3241 caacatcttg tgtctggcgg gccattgact cgcggccgga tcggtgctcc gcagggctgc
     3301 gcgcgggcct tgaaaaccgt cccggcgccc atattcacgg ctcatgaacc caaccccgat
     3361 caacgcacag ttcaggaggt aggccatgag cctgattcgt taccagccct ggggcctgat
     3421 gcgctccctg catgaagatc tcgaccgcct gttcgagcag cgcctcggca tggacgagac
     3481 caccggcagc gtcgccaact ggaccccgcc cgtggacatc cgggaagagg aaaaccgctt
     3541 cctgctgcat gccgacattc ccggcgtcaa gccggaggac atcgaagtca ccatggagaa
     3601 cggcgtgctg accatcagcg gcagccgcgc cgagacccgc gagcaggacg ccaacggcta
     3661 tcgccgcgtc gaacgcgtgg cgggacggtt cttccgtcgc ttcacgctgc ccgacaccgc
     3721 gaatgccgag ggcatcacgg cgaagagcac caacggcgtg ctcgaggtta tcatccccaa
     3781 gcatgagcgg gtgatgccgc gccggatcag cgtggacgtc aactgattgc ccgacgtgac
     3841 cggactgccg cgaccttttc ggcggcagcg gcatcttctg cgcagacggc gggcggcgcg
     3901 cagccgcccg tcgtccgtgt tgacgcgatg attttgaccc cgtgaggaac ctggccgcgc
     3961 cgccagcgtc caacagccac gttattccca gcagagaccc gagcccatga ctgcacgccc
     4021 cctcgccgct ctcgcggccg tcctctccct cgcggtcacc gcgccgctgt atgcgcaact
     4081 ccccgccgcg gtggacggcg cgccgctgcc ctcgctggcg ccgatggtga agaagaccgc
     4141 gccggccgtg gtcaacatcg ccacccgcgg cagcatcgag gtgcaagggg gcaacccgtt
     4201 cctcgatgat cccttcttcc ggcgcttctt cgacatgccc gaccagccgc gccagcggcg
     4261 ccagacccag agtgcgggct cgggcgtgat cgtaaatgcg gctgaggggc tggtgctgac
     4321 caacgcccac gtcgtcgagg gcgccgagga gatcagcgtc acgctgcagg acgaccgcac
     4381 cttcagggcc gaggtggtgg gcgcggaccg cgcttccgac gtcgcgctgc tgcggatccc
     4441 tgccgagaac ctggtcgagc tgaaaatggc cgactcgggt cgtatcgagg tgggtgattt
     4501 cgtggtcgcc atcggcaatc ccttcggcct gggccacacc gtcacctccg gcatcgtcag
     4561 cgcgctcggc cgctcgggca tcaaccccga gggttacgag gatttcatcc agaccgacgc
     4621 ctccatcaat ccgggcaatt ccggcggcgc gctggtcaac ctgcgcggtg aactggtggg
     4681 cgtgaactcc gccatcctgt cgcgcggcgg cggcaacatc ggcatcggct ttgcgatccc
     4741 ctcgaacatg gcgcgctcgg tcatggagca gctgctggag ttcggcgaag tgcggcgcgg
     4801 cctgctcggc gtcaccatcc tgaccatcac gccggacatc gccgaagcct atgacatcaa
     4861 ggaatcacag ggcgcgctgg tctcgcaggt gatgcccgaa tccgccgcgg agcgtgccgg
     4921 cgtcaaggtg ggcgacgtaa ttcgcagcgt cgacggcaag tcgatcagca acgccaacga
     4981 cctgcgcaac accatcggcc tcaagcgcag cggcgatacc gtcgagctgg agatcatccg
     5041 cgacaagcgc aagcgcacgg tcaaggccag gctcggtgag caggccacac aggcgcaggt
     5101 ctcggcccag gacatccatc ccgggctgca gggcgcggag ctcgagacgc tcgacgcctc
     5161 ctcgccgcgt tacgagggcg aggccggcgt gctggtgtcg aacgtcgagc ccggcagccc
     5221 cgccgcacag cgcggcctgc gcccgggcga cgtgatcatc ggcgtgaacc gtcgcaaggt
     5281 cagcaatctc gccgagctgc agcaggcggc ccagggccag gcgctgctgc tgaacatccg
     5341 ccgcggcaac gccaacctga tcctgccgat ccgctgaaaa accgggaaaa ccggggtcag
     5401 agacaggttt tcgcctcgcg aaaacctgtc tctgaccccg gttttcccga ccccggtttt
     5461 tcagccgcgg ccgccgatca gcatgtcggc ggccaccacc accagcagca cggcgaacag
     5521 gcggcgcagc tgcttgaccg gcagccggtg cgccaggcgc gccccgaggg gcgccgtggt
     5581 catggccgcc agcgcaatga cgccgatcgc cggccagtac aggtagccgg tcgcccagcc
     5641 cgggaccggt gcgccctggc cggccagcgc atagccgagc gcgcccgaca gcgcgaccgg
     5701 caaggtgcag gccgcggccg tgcccacggc cacgcgcagc ggcaccgagt gccaggcgag
     5761 gtacggcacg gtcatgatgc cgccgccgat gccgcccagc gcggagagcg agccgatcac
     5821 cgtgcccgcg ccgagatagc ccggcagccg cggcagcggc gcatccggct gcggctcgac
     5881 gcggccgccg aagccgatct gcaacgccat caggatggcg aacaggccga acaccacttc
     5941 cagcgagcgc gtcgcgagca ggtcggccag ccaggcgccc agcaggccgc cgagcacgat
     6001 gcccggcgcc agcccgcgca ccgccggcca cagcaccgcg ccgtagccgt ggtgtgcgcg
     6061 caccgaggaa atcgccgtga acacgatcac cgccagcgag ctgccgatgg cgacgtgcat
     6121 gcgcaccgcc ggctccatgg cgacgaggcc cgccgcgaac accgcgttga gcgccggcac
     6181 gatgaccagc ccgccgccga tgccgaacag gccggccagc agccccgaca gggcgccgat
     6241 ggcgggaaag atcaacagca gcgctggcag cgtcggcatc gtcggcccgg ttgagaacgg
     6301 cgaagcggcg agcataatgg ttagctcccg ggcccgcgag gcccgccctg ctcctggaga
     6361 gccgcgttca tgccgcgcat cgccctgctg acgaccctgg tcctgctgtt gacggccgca
     6421 ccggcgcacg caacctcccg cgaaagccag cgcgaagcgt tccgcgcggc gctgccccag
     6481 gccgaggcgg ggcgctggga ccgcgtcgaa ccgttgctgc cgcagctggc cggctacgcg
     6541 ctgctgcccg acctgcgcgc ggcgtggctg cgcagccagc tcggccgtgt gaacgacgcc
     6601 gaggtgcgcg aattcctggc gcgccatccg gatctggcct tcagcgcccc gctgcgccgc
     6661 cagtgggccg gctccctggc gcgccgcggc gcctggcgcg agtacctcga gctgttcaag
     6721 gcccactacg ccgacagtca cgaggcggtg ctggagtgcc attccttcac cgcccgcatc
     6781 cggctggatc gcactgccgg cctcgaggaa gcggtgctgc gccgctggct gtggcccacc
     6841 agccaggtga aggaatgtga tcccgccttc gcgtggctgg agcagcaggg cgccatcacg
     6901 ccggagcgcc gccgcgcgcg catggagctg gcgctcggcg ccggcgagtt ccgcctggcg
     6961 cggtggctgg cgcggcccct cggcgccgcg gccgtcaacg aagtggatac ctgggagcgg
     7021 ctgcatgccg acccggcggg gcggctcggt tcgccggccg gctggaagga ttcgccacgc
     7081 gcgcgcgcca tgctgctgta cggcttccac cggctggcct ccagcgaccc ggaagcggcc
     7141 gcgcgccgct ggccggcctt ccgcgaccac ttctccttcg acgccgacga acgcgcgcgc
     7201 atcgaccggc gcatcgcgct catgcacgcc tggcggcacc tgccgggcgc ttaccagatg
     7261 ctgatcgcat tgccggaggc cagccaggac gacagcacgc gcacctgggc ggtgcgcgtc
     7321 gccattcgcg cccaggactg gagcgcgatc gaagcggcca tcgcccggct cgatccggtg
     7381 accgcgcagg aaccggcctg gcgctactgg caggcgcgta cgctcgaggc gaccggccgc
     7441 agcgatgcgg cccggccgat ctacgccgaa ctggccggtg agcgcggcta ctacagcttc
     7501 atgtcggcgg accggctgaa tgccgactac aactggcagc acacgcagac cccgccggac
     7561 gagcgcctga tctcagccct cgaggagcgc gccgatatcg tgcgtgcgcg ggagctgttc
     7621 atgaccggcc aggactcgca cgggcggacc gagtggcagc aggcgc
//
LOCUS       DAOMPG010000108         7622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4151_length_7622_cov_4.129245, whole genome shotgun sequence.
ACCESSION   DAOMPG010000108 DAOMPG010000000
VERSION     DAOMPG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4151_length_7622_cov_4.129245"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1022)
                     /locus_tag="PLS34_07245"
     CDS             complement(93..1022)
                     /locus_tag="PLS34_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRP87299.1"
                     /translation="MDRLQAMETFVRVVELGNFSAAARQAGVARSVVTRQVAALEKHL
                     GVKLLNRTTRRLSLTSAGTRYLEKCRVILNLAEAAETDLAEERAELRGRIRIGLPLSF
                     GLKRVAPLLLEFAAEHPEVSLEADYTDRRLNLIEEGFDLSVRVTSRLAPGDVSRRLGR
                     CRIVAVAAPDYLARHGTPAHPAELEGHECLAYLGDANPNLWSFDTGGRIESFAIRSRF
                     SASNGEALLAAAAQGFGITLQPDFIVDDWLADGRVVPVLASFPAPGLGVYALLPSGTH
                     MPFRVRALVEYLASRLKGEQADVDPGRPYGSKA"
     gene            1121..1936
                     /locus_tag="PLS34_07250"
     CDS             1121..1936
                     /locus_tag="PLS34_07250"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRP87300.1"
                     /translation="MTINTTLPSLFVPHGAPTFALRPGAAGAALARQAAALPRPRAVV
                     AVSAHWETARPTVGTAPRLDTVHDFRGFPEALYRIRYPATGCPEAARAVGRAIEAAGL
                     PVDFDAERGLDHGAWVPLRMMFPAADVPIVPLSLQPDAGPAHALRVGRALAPLRREGL
                     MVIASGNLTHNLRDYFEAGPDRAEPPAYVRLFADWMHQRLVAGDTAALLDYRRQAPDA
                     RRAHPSEEHLLPLFVAPGATGEPFETLRFHAGIDDAVLAMDAFAFTARHDAVH"
     gene            1979..2557
                     /locus_tag="PLS34_07255"
     CDS             1979..2557
                     /locus_tag="PLS34_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRP87301.1"
                     /translation="MQTRPYASAVLVLAALLGAPALAAPQTYLIDDAHTFARFSYSHF
                     GLSTQQSRFNRTSGAIVLDKAARTASVEIEIDMTTVDTGDAEFDQHIQAEDFFHTARF
                     PTATFKSTRVDFDGEVPTAVHGDLTIKGITRPVTLAISNFLNMPHPMVEKDAIGADAG
                     VIVKRSDFDAGKYAPYVGDEVTISIALEAIVQ"
     gene            2575..3174
                     /locus_tag="PLS34_07260"
     CDS             2575..3174
                     /locus_tag="PLS34_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRP87302.1"
                     /translation="MNILQVNGSVRANGSNSTRLADAITARLRSQQPDACLVLRDLAT
                     HPHPVLDAAALAALSTPATERTAEQAARVALDDALIAEVQAADVIVLGVPMYNFGIPA
                     QLKAWIDAIARAGVTFRYTEQGPEGLLCNKKVYAGLARGGLYRDTPADSQVPYLRNVL
                     GFFGLEDIEFVYAEGLNMGPESARKALLEAEAHIEKLVA"
     gene            complement(3203..3754)
                     /gene="pgsA"
                     /locus_tag="PLS34_07265"
     CDS             complement(3203..3754)
                     /gene="pgsA"
                     /locus_tag="PLS34_07265"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003208929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRP87303.1"
                     /translation="MNIPNTLTWIRIILIPLIVVVFYLPYAWSGPAAAIIFSLAGITD
                     WLDGYFARKLDQASAFGAFLDPVADKLMISTALVLLVSKHPGYVVALPAAIIIGREIA
                     VSALREWMSELGGRHHVKVSGIGKYKTTAQITALIMMLWGQKLWFIPMYQIGYGFLLA
                     AALLTLWSMWNYLRAAWPHLRGY"
     gene            complement(3772..5640)
                     /gene="uvrC"
                     /locus_tag="PLS34_07270"
     CDS             complement(3772..5640)
                     /gene="uvrC"
                     /locus_tag="PLS34_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRP87304.1"
                     /translation="MSKRRQPAGEAAPAKAATDFDPRPVLKRLGHRPGVYRMLGADGD
                     ILYVGKSRDLKKRVSSYFRGTPRPGKTGFLVSQVRDIEVTVTRSEAEALVLENTLIKA
                     HRPRFNVLLKDDKSFPYIRLDTSHAYPRLSFYRGTRRVPGRLFGPYPNVPAVRATLRD
                     LQRLFRIRPCEDSYFAHRSRPCLQYQIERCSGPCVGLISEQDYARDLDHAIRFLEGRS
                     HEIKADLRRRMEAAAAQLAFEQAARYRDQIGRLAAIQESAVGGEAGALEADALAVYEQ
                     GGLFCVAAMFIRGGRILGSRSWFPRATPDTEPAEVLGAFVAQHYLGSSPPAEILTTLA
                     LEDEALLSTALSEQAGRQVAVRHRVRAPRSRWLEMAAENARQALELRIAGTAGFSAQF
                     AALGEALGLDDPPQRIECFDISHTGGEATVASCVVFGPEGPLKSDYRRFNISGVAAGD
                     DYAAMRQALQRRYARVKRGEAPMPDLLLVDGGAGQLAEAQSVLNELQLDGVELVAVAK
                     GPTRRPGLERLFLAGQPEPLILPRDSGALHLVQQIRDEAHRFAIAGHRQRRARVRRVS
                     PLEGIAGLGPKRRRQLLSELGGMQGVVAAGVEDLARVQGISRDLAQRIYDALHPVE"
     gene            complement(5637..6314)
                     /locus_tag="PLS34_07275"
     CDS             complement(5637..6314)
                     /locus_tag="PLS34_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRP87305.1"
                     /translation="MSLKSPTGDSYPATWFDPGCRRCARLAGFLDEVRDQHPDYHAAP
                     VPPFGPRAAALLIVGLAPGMHGANRSGRPFTGDHAGILLYETLHRLGLASGPASVSRD
                     DGLTLSGCRITNAVKCLPPANKPTPAEVRSCRRWLTAELEEGRPRVILALGRIAHDGV
                     LDALGLRRAAHPFAHGAEHDLGAAGRLVDSYHCSRYNTQTRRLTPEMFRAVMARAATL
                     AGVGRVP"
     gene            complement(6354..>7622)
                     /gene="icd"
                     /locus_tag="PLS34_07280"
     CDS             complement(6354..>7622)
                     /gene="icd"
                     /locus_tag="PLS34_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRP87306.1"
                     /translation="LNVPDRPIIPFIEGDGIGIDVTPVAIKVVDAAVRAAYGDRRSIA
                     WMEIFAGQKATGLYGGSQFLPEETLDALERFVVSIKGPLATPVGGGFRSLNVSIRQRL
                     DLYACVRPIRYFPGVQTPMKESELVAMTVFRENTEDIYAGIEWPAGSPEAQKLMKFLQ
                     DELNVHAVRFPSSSGLGIKPVSKEGSQRLIRKAINYAVDEDRSSVTLVHKGNIMKFTE
                     GAFRNWGYQLARDEFGAVELGDGPWLAFNNPRTGREIVIKDVIADNFLQQILLSPEEY
                     DVIATLNLNGDYISDALAAQVGGIGIAPGANIGDAVAVFEATHGTAPGFAGKDRVNPG
                     SLILSAEMMLRYLGWREAADLILRGVARTIANGELTFDLARLREAIRKPKKGEARGSV
                     EEELQRLVPGAKLVGTTGFGDAIIRHMQEE"
BASE COUNT         1179 a         2703 c         2589 g         1151 t
ORIGIN      
        1 aactcgcgac cccaaccttg gcaaggttgt gctctaccaa ctgagctatt cccgcttcgg
       61 gacgcggaat tctaagctac ccgccctccc tgtcaagcct tgctcccgta aggccgaccc
      121 ggatccacgt cggcctgctc gcccttgagg cgactcgcca ggtactccac cagcgcccgc
      181 acgcgaaacg gcatgtgcgt gccgctcggc aacagcgcgt acacgcccag cccgggcgcc
      241 gggaacgacg ccagcaccgg cacgacccgg ccgtcggcca gccaatcatc cactatgaag
      301 tccggctgca gggtgatgcc gaagccctgc gctgccgccg ccagcagcgc ttcgccgttg
      361 ctggcgctga aacggctgcg aatggcaaag ctctcgatgc gcccgccggt gtcgaaggac
      421 cagaggttcg ggttggcgtc gcccaggtag gcgaggcact cgtggccctc gagctcggcg
      481 gggtgcgccg gcgtgccgtg acgcgcgagg tagtccggcg cggccaccgc gacgatccgg
      541 cagcggccga ggcgcctcga cacgtccccc ggcgccagcc gggatgtgac ccgaaccgac
      601 aggtcgaagc cctcttcgat caggttcagg cgccggtcgg tgtagtcggc ctcgagactc
      661 acctcggggt gctccgcggc gaactccagc aacagcggcg cgacccgctt gagtccgaag
      721 ctcagcggca ggccgatgcg gatgcggccg cgcagctcgg cgcgttcctc ggcgagatcg
      781 gtttccgccg cttccgccag attcaggatc acgcgacact tctccaggta acgggtcccg
      841 gccgaggtga ggctgagccg ccgggtcgtg cggttgagga gcttgacgcc gagatgcttc
      901 tcgagcgccg ccacctggcg ggtgaccacc gagcgcgcca cgccggcctg ccgggcggcg
      961 gcggagaaat tccccaattc caccacccgc acgaaggttt ccatggcctg caggcgatcc
     1021 attgttgcat atcgtgcaac aaagaatccc tctttgtcac ctatttcgat aacaaaaact
     1081 gatgcactct gctcaccgaa ccctgcatgc cgtgacgaat atgaccatca acaccaccct
     1141 gcccagcctg ttcgtgccgc acggcgcgcc caccttcgcc ctgcgacccg gcgcggccgg
     1201 cgccgcgctc gcgcggcagg ccgccgccct gccccggccg cgtgccgtcg tcgccgtgag
     1261 cgcgcactgg gagaccgcgc gcccgacggt cggcactgcg cctcgcctgg acaccgtgca
     1321 cgacttccgg ggtttcccgg aggcgctgta ccgaatccgc tacccggcca cgggctgccc
     1381 cgaggccgcc cgggccgtcg gtcgggcgat cgaggccgct ggcctgccgg tcgatttcga
     1441 cgccgagcgc gggctcgacc acggcgcctg ggtccccctg cgcatgatgt tccccgcggc
     1501 cgacgtgccg atcgtgccct tgtcactgca gccggacgcg ggcccggcgc acgcgctgcg
     1561 ggtgggccgg gcgctcgcgc ccctgcgacg cgagggcctg atggtcatcg cgtcgggcaa
     1621 cctgacccac aacctgcgcg attatttcga ggccgggccc gatcgtgccg agcccccggc
     1681 ttacgtccgc ctgtttgccg actggatgca tcagcggctc gtggcaggtg ataccgcggc
     1741 gctgctcgat taccgcaggc aggcgcccga tgcccggcgc gcgcacccca gcgaagaaca
     1801 cctgctgccg ctgttcgtgg cgcccggcgc caccggcgag ccgttcgaga cgctgcgttt
     1861 ccatgccggc atcgacgacg cggtgctggc catggacgcc ttcgccttca ccgcccgcca
     1921 cgatgcggtg cactgagcct ttcttggatc tgtaactcac cacatggagc ccaacccgat
     1981 gcaaacccga ccctacgcgt ccgccgtcct cgtgctggcc gctttgctgg gcgcacccgc
     2041 gctggcggcg ccgcagacct acctgatcga cgacgcccac accttcgcgc gcttctccta
     2101 cagccacttc gggctgtcga cccagcagag ccgcttcaac cgcaccagcg gcgcgatcgt
     2161 gctcgacaag gcggcgcgca cagcctccgt ggaaatcgag atcgacatga ccacggtcga
     2221 taccggcgac gcggaattcg atcagcacat ccaggccgag gacttcttcc acaccgcccg
     2281 cttccccacg gcgacgttca agtccacacg cgtggatttt gacggcgagg ttcccacggc
     2341 ggtacacggc gacctgacca tcaagggcat cacccggccg gtgacgctcg cgatcagcaa
     2401 cttcctcaac atgccgcacc cgatggtcga gaaggacgcc atcggcgccg acgccggcgt
     2461 aatcgtcaag cgcagcgact tcgacgccgg caagtacgcg ccctacgtcg gcgacgaagt
     2521 gaccatcagc atcgcgctcg aggcgatcgt gcaatgagca cccggagcaa gaccatgaac
     2581 atcctgcagg tcaacggcag cgtccgcgcc aacggctcga actcgacccg gctggcggac
     2641 gcgattaccg cccggctgcg ctcgcaacag ccggacgcgt gcctcgtgtt gcgcgatctc
     2701 gccacgcatc cgcacccggt gctcgacgcg gcggcgctgg cggcgctttc cacgcccgcc
     2761 actgagcgca cggccgagca ggccgcgcgc gtggcgctcg acgacgcgct gatcgccgaa
     2821 gtgcaggcgg ccgacgtcat cgtgctgggc gtgccgatgt acaacttcgg catcccggcc
     2881 cagctcaagg cctggatcga cgccatcgcc cgcgccggcg tgaccttccg ttacaccgag
     2941 cagggcccgg aggggctgct gtgcaacaag aaggtctacg ccggcctggc ccgcggcggg
     3001 ctctatcgcg acaccccggc cgactcgcag gtgccctacc tgcgcaacgt gctgggcttc
     3061 ttcggcctcg aggacatcga gttcgtttac gcggaggggc tcaacatggg accggagtcc
     3121 gcgcgcaagg cgctgctcga ggccgaagcg cacatcgaga agctggtcgc ctgacgggga
     3181 atcaagcaag cctgcgggag gctcagtagc ctcgcaagtg gggccaggcc gcccgcaggt
     3241 agttccacat ggaccacagc gtcagcagcg ccgcggccag caggaagccg tagccgatct
     3301 ggtacatcgg gatgaaccag agtttctggc cccagagcat catgatcagc gcggtgatct
     3361 gcgcggtggt cttgtacttg ccgatgccgg acaccttgac gtggtgccgg ccgccgagct
     3421 cggacatcca ctcgcgcagc gccgagaccg cgatttcgcg gccgatgatg atcgccgcgg
     3481 gcagcgccac cacgtagccc ggatgcttcg agaccagcag caccagcgcg gtggaaatca
     3541 tcagcttgtc ggccaccggg tcaagaaagg cgccgaaggc cgaggcctgg tcgagcttgc
     3601 gggcgaaata gccgtccagc cagtcggtga tgcctgccag cgagaagatg atcgccgccg
     3661 ccggcccgga ccaggcatag ggcaggtaga acaccaccac gatcagcggg atcaggatga
     3721 tgcgaatcca ggtcagggta ttggggatgt tcagcctggc tggcatgtcg gtcattccac
     3781 cggatggagt gcgtcgtaaa ttcgttgcgc caggtcgcgg ctgatgccct ggacccgggc
     3841 gaggtcctcg acgccggccg ccaccacgcc ctgcatgccg ccgagctcgc tgagcagctg
     3901 ccgccggcgc ttcggcccca acccggcgat gccctccagc gggctgaccc gtcgcacccg
     3961 cgcgcgccgc tgccggtgcc cggcgatggc aaaccggtgc gcctcgtcgc gaatctgctg
     4021 caccaggtgc agcgcgcccg aatccctcgg cagtataagt ggctcgggtt ggccggccaa
     4081 gaacagccgc tcgagacccg ggcgtcgcgt cgggcccttg gccaccgcca ccagctcgac
     4141 gccgtcgagc tgcagctcgt tgagcacgga ttgcgcctcg gcgagctggc cggcgccgcc
     4201 gtccaccagc agcaggtcgg gcatcggcgc ctcgccgcgc ttgacccggg catagcggcg
     4261 ctgcagcgcc tggcgcatgg cggcgtagtc gtcgccggcc gccacgccgg aaatgttgaa
     4321 gcgccggtaa tcggacttga gcggcccttc cgggccgaac accacgcagg aagcgacggt
     4381 cgcctcgccg ccggtgtggc tgatgtcgaa acactcgatc cgttgcggcg gatcgtccag
     4441 gccgagcgcc tcgccgagcg cagcgaactg cgcactgaag cccgcggtgc cggcgatgcg
     4501 gagctccagc gcctggcggg cgttctccgc ggccatctcc agccagcgag aacgcggcgc
     4561 ccgcacgcgg tggcgcacgg ccacctggcg cccggcctgc tccgacaatg ccgtggacag
     4621 gagcgcctcg tcctccagcg cgagggtcgt gaggatctcc gcgggcgggc tgctgccgag
     4681 gtagtgctgc gcgacgaagg cgccgagcac ctctgcgggc tcggtgtccg gcgtggcgcg
     4741 cggaaaccag ctgcggctgc cgaggatgcg gccgccgcga atgaacatgg ccgcgacgca
     4801 gaacaggccg ccctgctcgt aaaccgccag cgcgtccgct tccagcgcgc ccgcctcgcc
     4861 gcccacggcg gactcctgga tagccgccag gcggccgatc tggtcgcggt agcgcgcggc
     4921 ttgctcgaag gcgagctgcg cggccgctgc ttccatccgg cggcggaggt cggccttgat
     4981 ctcgtggctg cggccctcca ggaagcggat cgcgtgatcc aggtcgcgcg catagtcttg
     5041 ttcggaaatc agcccgacgc agggccctga gcagcgctcg atctggtact gcaggcaggg
     5101 gcggctgcga tgcgcgaagt agctgtcctc gcagggccgg atgcggaaca accgctgcag
     5161 gtcgcgcagc gtggcgcgca cggccgggac gttcgggtac ggtccgaaca gccgccccgg
     5221 gacgcggcgc gtgccgcggt agaaggacag ccgcgggtag gcgtgcgagg tgtcgaggcg
     5281 gatgtaggga aagctcttgt cgtccttgag caggacgttg aagcgcggcc ggtgcgcctt
     5341 gatcagcgtg ttctcgagca ccagcgcctc ggcctcgctg cgcgtcaccg tcacctcgat
     5401 gtcgcgcacc tggctgacca ggaagccggt cttgccgggc cgcggcgtgc cgcggaaata
     5461 gctcgagacg cgctttttca ggtcgcgcga cttgccgacg tagaggatgt cgccgtctgc
     5521 gcccagcatc cggtagacgc ccggccggtg gccgagccgc ttgagcaccg ggcgcggatc
     5581 gaagtccgtc gcggcctttg ccggcgccgc ctcgccggcg ggctgccgac gcttgctcat
     5641 gggacccgcc ccacgccggc gagcgtggcc gccctggcca tgaccgcgcg gaacatctcc
     5701 ggtgtgaggc ggcgcgtctg ggtgttgtag cggctgcagt ggtaggaatc gaccagccgg
     5761 cccgccgccc cgaggtcgtg ctctgcgccg tgtgcgaagg gatgcgcagc ccgccgcaag
     5821 ccgagcgcat ccagcacacc gtcgtgcgcg atccgcccca gcgccaggat cacccgcggc
     5881 cggccctcct ccaattccgc cgtcagccag cgacggcagc tgcgcacctc ggccggcgtg
     5941 ggcttgttgg ccggcggcag gcatttcacg gcgttggtga tgcggcagcc ggacagagtc
     6001 agaccgtcgt cgcgcgacac ggatgccggc ccgctggcca ggccgagccg gtgcagggtc
     6061 tcatagagca ggatgccggc gtgatccccg gtgaacggcc ggccgctgcg gttggcgccg
     6121 tgcatgccgg gtgcgaggcc gacgatgagc agcgccgccg cgcgcgggcc gaagggcggc
     6181 accggtgcgg cgtggtagtc gggatgctgg tcgcggacct cgtcgagaaa gccggccagc
     6241 cgcgcgcaac gccggcagcc cgggtcgaac caggtggccg ggtagctgtc tccggtgggt
     6301 gacttcagcg acaattcact tcaaatagca tccgcaatcc tatgatatag aggctattcc
     6361 tcttgcatgt ggcggatgat ggcgtcaccg aatcctgtgg tcccgaccag cttggcgccg
     6421 ggcaccagcc gctgcagctc ctcttccacg ctgccccggg cctcgccctt cttcggcttg
     6481 cggatggcct cgcgcaggcg cgccaggtcg aaggtcagct cgccgttggc aatggtgcgc
     6541 gccacgcccc tgaggatcag gtcggcggcc tcgcgccagc cgaggtagcg cagcatcatc
     6601 tcggccgaca ggatcagcga gcccgggttg acgcggtcct tgccggcaaa gcccggcgcg
     6661 gtgccgtggg tcgcttcgaa caccgccacg gcgtcgccga tgttggcgcc cggcgcgatg
     6721 ccgatgccgc ccacctgggc ggccagcgcg tcggagatgt agtcgccgtt gaggttcagc
     6781 gtggcgatga cgtcgtactc ctccgggctc agcaggatct gctgcaggaa gttgtctgcg
     6841 atcacgtcct tgatgacgat ctcgcggccg gtgcgcgggt tgttgaaggc cagccacggg
     6901 ccgtcgccca gctcgacggc accgaactcg tcgcgcgcca gctggtagcc ccagttgcgg
     6961 aaggcgcctt ccgtgaactt catgatgttg cccttgtgga ccagcgtcac cgagctgcgg
     7021 tcctcgtcca cggcgtagtt gatggccttg cggatcagcc gctgcgagcc ctccttggag
     7081 accggcttga tgccgaggcc ggagctcgac gggaagcgca ccgcatggac attgagctcg
     7141 tcctgcagga acttcatcag cttctgcgcc tcgggcgagc ccgccggcca ctcgatgccg
     7201 gcgtagatgt cctcggtgtt ctcgcggaac accgtcatgg cgaccagctc ggattccttc
     7261 attggcgtct gcacgccggg gaagtagcgg atcggccgca cgcaggcgta caggtcgagg
     7321 cgctggcgga tcgagacgtt cagggagcga aagccgccgc ccaccggcgt cgccagcgga
     7381 cccttgatgg acaccacgaa gcgctccagc gcgtcgagcg tctcctcggg caggaactgg
     7441 ctgccgccgt acaggccggt ggccttctgg ccggcaaaga tttccatcca ggcgatcgag
     7501 cgccggtcac cgtaggccgc gcgcaccgcc gcgtccacga ccttgatggc caccggcgtg
     7561 acgtcgatgc cgatgccgtc gccctcgatg aagggaatga tcgggcgatc ggggacgttg
     7621 ag
//
LOCUS       DAOMPG010000109         7622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4152_length_7622_cov_3.971059, whole genome shotgun sequence.
ACCESSION   DAOMPG010000109 DAOMPG010000000
VERSION     DAOMPG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_7622_cov_3.971059"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLS34_07285"
     CDS             complement(<1..159)
                     /locus_tag="PLS34_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRP87307.1"
                     /translation="MALHVVILAAGQGTRMKSALPKVLQPLAGRPLLAHVLGRALGLG
                     AHATHVVYG"
     gene            complement(398..1738)
                     /locus_tag="PLS34_07290"
     CDS             complement(398..1738)
                     /locus_tag="PLS34_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRP87308.1"
                     /translation="MNGAPDEEPLLRQLSAAGIWLLAVNGMIGAGIFGVPAGAAALTG
                     IYSPLVFLGCGFLLTAVVLCFAELASYFRGTGGPVLYLRSAFGPMAGFQAGWAFYIAR
                     ATAFAANLNLLVASLAYFWSPATGGPVRVLLMAAIVALLAWPNIAGTRHAMRSLGLLP
                     LLKLLPLLAVVGFGAVQLGPASFPVGSAPLPDHAAFGTAALLVIYAFVGWESAVVPGG
                     ETRNPARDMPRALILAMVMVTALYVLIQAVAVAVLPDLAASERALVDVGAALFGPAGA
                     VVLTVAVLFSVGGNLAGSMVTAPRLTYALAREGSLPAWLARVHPEARTPSASILCYAV
                     LVLLLAVYGSFVWLAAMSALVRVLIYMACIGAMPRLRRRLGAVPGGFRVPGGGFTALG
                     AFLVSGLLLLQVRAAAVLVTAALLLAGALIQAWVRRGRPADDAPGELPPERPVE"
     gene            complement(1762..2205)
                     /locus_tag="PLS34_07295"
     CDS             complement(1762..2205)
                     /locus_tag="PLS34_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87309.1"
                     /translation="MRTLYRHKQFGGFSLLGAGAGVLLILALVGQLGAHPMLLVVLGL
                     IVMVGVMLSSLTVVVTPSSLVFWFGPGVLRKTVPLAEVEKVEAVRNPWYYGIGLRATP
                     RGMLYNVSGLSAVEVTRRDGGRFRVGTDEPEKLCASLRQAMARFE"
     gene            complement(2354..2824)
                     /locus_tag="PLS34_07300"
     CDS             complement(2354..2824)
                     /locus_tag="PLS34_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRP87310.1"
                     /translation="MRNTTAPGPFACGVACAVLLLALPVATAGADSHGGKALARSMDD
                     PRLEWGPCPEFLPAGCAIAVLHGDPAGPNADIFFKVPGGADIPRHQHTSAERMVLVSG
                     QLEVTYDGQDPVVLRPGDYAYGPPGLPHTGRCASGDPCVLMIAFEEPVDAVPVE"
     gene            complement(2970..4133)
                     /locus_tag="PLS34_07305"
     CDS             complement(2970..4133)
                     /locus_tag="PLS34_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HRP87311.1"
                     /translation="MPKSLIRVVWLAALIGAGCATSPPATTPGGEAPPWLQSLTAAVG
                     DRRLLQLGESGHGMAETYRLKTEIVRHLHERHGFDVLAVEGGIAECWVAAQRMAEWTA
                     EQSMRACFWGAWASEEAEALFEYLRSATAAGRPMRLVGFDNSPTSRAFSSWIEATPGL
                     PPTLAAAERSFLRIFAGDFEGEEGLAEIRRRAAAGFQAAREATTEPMLAMIIEDRLAT
                     LDFNPAEFDRAAFERLREQRMAVNLLRHIQREPDARIIVWAHNAHIAHAYSRFVGGVP
                     RQGEFVQGALGDAGYTLGIYPLGGRGYAWFINQDYDLRPPEADSLEARLAAMPGDPVF
                     LDLRGTAAERHPWIRQPVKSFEWGLNEQLIVPAEMYDGLLLIREVTPITRSGD"
     gene            complement(4210..5268)
                     /gene="pseI"
                     /locus_tag="PLS34_07310"
     CDS             complement(4210..5268)
                     /gene="pseI"
                     /locus_tag="PLS34_07310"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HRP87312.1"
                     /translation="MTLPSIHIAGRRIAADAPPYVIAEMSANHHGRLETALAIVDAAK
                     RAGADAVKVQTYRPDTITLDSGAEDFRIKGGLWDGRTLYELYQEAHMPWEWHAPLFAH
                     ARKAGITIFSSPFDATAVDLLESLDAPAYKIASFEAVDLPLIRYVAATGKPMIISTGM
                     ADADEIGEAIAAARDGGCRELAILHCVSGYPAPAADYNLRTLADMRHRFGVVTGLSDH
                     TLDNTTAIAAVALGAALIEKHFTLDRHGGGPDDTFSLEPADLAALCKGARTAWETLGQ
                     VDYGRKSSEQGNAQFRRSLYFVKALRAGDTVTADAVRSVRPGFGLAPKHLDRVLGRRL
                     ARDVAASTPVREDDLDPP"
     gene            complement(5265..5825)
                     /gene="pseH"
                     /locus_tag="PLS34_07315"
     CDS             complement(5265..5825)
                     /gene="pseH"
                     /locus_tag="PLS34_07315"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HRP87313.1"
                     /translation="MSTGPGDAVRRMTADDLERVLRWRNHPDVRACMFTRHEISPEEH
                     ARWFAAAAADPARHLLIFERDGAPLGFASLAVGAAPRVAAWGFYLAPEAPRGSGAALG
                     AAVLEHAFGVLGVDEVRGEALAFNTPSIRMHEKLGFHRAGERRQPDEHGEGEQAVICF
                     QLQRAAWLAGARPAGPSRHRHGDPTP"
     gene            complement(5822..6910)
                     /gene="pseG"
                     /locus_tag="PLS34_07320"
     CDS             complement(5822..6910)
                     /gene="pseG"
                     /locus_tag="PLS34_07320"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HRP87314.1"
                     /translation="MRFAFRADASIRIGSGHVMRCLTLADSLRARGHESRFLARPHEG
                     HLAEHIRARGHAVLLLPVDGAEHDEADPDGPPHADWLGGPWTEDATASLAALRDAPPD
                     WLVVDHYALDARWEALVAPACGRTLVIDDLADRPHACDLLLDQNLGRRAADYAALLPA
                     GCETLIGQQFALLRPEFRALRAASLARREAPLLRNLLVSMGGIDKDDATSAVLDGIEA
                     AGLPDGVTVTVISGRDSPWLDKVRRRAADMRLPTTVLVDVRNMAERMLQADLAIGGAG
                     GTAWERCCLGLPSIVVVLADNQRPGAEALARAGAAWVIDEVAAVPRELPGLLATAVAP
                     AVLAQAARRAAGITDGRGAERVSERLLS"
     gene            complement(6907..7608)
                     /gene="pseF"
                     /locus_tag="PLS34_07325"
     CDS             complement(6907..7608)
                     /gene="pseF"
                     /locus_tag="PLS34_07325"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HRP87315.1"
                     /translation="MRLAVIPARGGSKRIPRKNIREFCGRPIIAWSIEAALQSGCFDR
                     LVVSTDDEQIAAAARACGAEVPFLRPAALADDHAGTLAVMRHAVETLAVDRQAGRVCC
                     IYATAPFLRAEDLRRGLETLEQSGADYSFSVTSFPFPIQRALRMTPQGRVEMFDPAQF
                     ATRSQDLEEAWHDAGQFYWGRAAAWLDEAPLFGHGAAPVILPRHRVQDIDTPEDWLRA
                     EWLFRAMQAERGQPE"
BASE COUNT         1184 a         2781 c         2649 g         1008 t
ORIGIN      
        1 gccgtacacc acgtgcgtgg cgtgcgcgcc gagcccgagc gcgcggccca gcacgtgcgc
       61 cagcagcggc cggccggcca gcggctgcag caccttgggc agggccgact tcatccgggt
      121 gccttgcccg gcggcaagga tgacgacgtg tagggccatg gggggcgatg tccgttggcg
      181 caggatggcg catgatacct gttttgcggc ggcctccgaa gggcgccggg cgacggattc
      241 cggcaccccg tccatgccgt gccaccaata gttatccaca atagcatggc gtgtctagtg
      301 gcctgtgtat aactttccgc gatcgggcct ttgggcggac gaagaacgga aaccttcata
      361 gagaacaagg ctttggggac ggctctcctg tgggtaacta ttccacaggt cgctcgggcg
      421 ggagctcgcc gggcgcatcg tcagccggcc ggccgcgccg cacccaggcc tggatgagcg
      481 cgccggccag cagcaaggcc gccgtcacca gcaccgccgc cgcgcgcacc tgtagcagca
      541 gcaggcccga caccaggaag gcaccgagcg ccgtgaaccc accgccgggc acgcggaagc
      601 cgcccggcac ggcgccgaga cggcggcgca gccgcggcat ggcaccgatg caggccatgt
      661 agatcagcac gcgcaccagt gcgctcatgg ccgccagcca cacgaaggag ccgtacacgg
      721 ccagcagcag caccagcacg gcgtagcaca ggatggatgc gctcggcgtg cgcgcctcgg
      781 gatgcaccct ggcgagccag gcgggcaggc tgccctcgcg cgccagcgcg taggtcaggc
      841 gcggcgccgt gaccatggag ccggccaggt tgccgccgac cgagaacagc acggcgaccg
      901 tcaacaccac ggcccccgcc gggccgaaca gcgccgcgcc gacgtccact agcgcccgct
      961 ccgaggccgc gagatccggc agcaccgcca ccgccacggc ctggatcagc acatacagcg
     1021 ccgtcaccat caccatcgcc aggatcagcg cccggggcat gtcgcgggcc ggattgcggg
     1081 tctcgccgcc gggcacgacg gcggattccc agccgacgaa ggcatagatc accagcaggg
     1141 ccgccgtgcc gaaggccgca tggtccggca aaggggccga ccccaccggg aagctcgccg
     1201 gcccgagctg cacggcgccg aaccccacca cggccagcag gggcaacagc ttcagcaggg
     1261 gcagcaggcc gagcgagcgc atggcgtgcc gtgtgccggc gatattcggc caggccagca
     1321 gcgcgacgat cgccgccatc agcagcaccc gcaccggccc gcccgtggcg ggcgaccaga
     1381 agtatgcaag cgacgccacc agcaggttca ggttggccgc gaacgcggtg gcgcgcgcga
     1441 tgtagaaggc ccagcctgcc tggaatccgg ccatcggacc gaaggcgctg cgcaggtaca
     1501 ggaccgggcc gccggtgccg cgaaagtaac tcgccagctc ggcgaagcag agcaccacgg
     1561 ccgtcagcag gaagccacag cccaggaata ccagcgggct gtagatgccg gtgagtgccg
     1621 ccgcaccggc cgggacgccg aagatgccgg caccgatcat gccgttgacc gccagcagcc
     1681 agatgcccgc agcggagagc tgccgcagca gcggctcctc gtcgggcgcg ccgttcactg
     1741 ctgcgggccg cgccccgggg ctcactcgaa gcgtgccatc gcctgcctga gactcgcgca
     1801 aagcttctcc ggctcatcgg tgccgacgcg gaagcgcccg ccgtcgcgcc gcgtaacctc
     1861 gaccgccgac aggcccgaca cgttgtacag catgccgcgc ggcgttgcgc gcaggccgat
     1921 gccgtaatac caggggtttc tcaccgcctc gactttctcg acttcggcca gcgggacagt
     1981 cttgcgcagc acgcccgggc cgaaccagaa caccagcgac gagggcgtca ccacgacggt
     2041 cagcgacgac agcatcacgc cgaccatcac gatcagcccc agcaccacca gcagcatcgg
     2101 gtgcgcaccc agctgtccca ccagcgcgag gatcagcagc accccggcac cggccccgag
     2161 cagggagaac ccgccgaact gcttgtgccg gtagagcgtc cgcacggcta gccgccgccc
     2221 aggacgatgc cgaccgccag gaattcagcg aagttcggcc tggccatgac atcctcgctc
     2281 tatccttgag tacccctcac agggtacggc attttcggca tgccgtggcg ccgacgaagg
     2341 tcgccggagt gtgctactcc accggcacgg cgtcgaccgg ctcttcgaag gcgatcatga
     2401 gcacgcaggg atcaccgctg gcgcagcgcc cggtgtgcgg cagtcccggc ggtccgtacg
     2461 cgtagtcccc ggggcgcagc accaccgggt cctggccgtc ataggtcact tccagctggc
     2521 cggagaccag caccatgcgc tccgccgagg tgtgctggtg gcgtggaata tcggcgccgc
     2581 cgggtacttt gaagaatatg tccgcgttgg ggccggccgg atcgccgtgc agcacggcga
     2641 tggcgcaacc ggccgggagg aactccgggc agggccccca ttcgagccgc ggatcgtcca
     2701 tcgacctggc cagtgctttt ccgccgtgcg agtcggcgcc ggcggtcgcc accgggagcg
     2761 cgagcaggag aacggcgcag gcgactccgc aggcaaatgg tcccggagcg gtcgtgtttc
     2821 gcatgatgaa tctctcttgg tgatgagttt gccggacgag gcacatgcag ccggcaccgc
     2881 cccccggcag ggctgcaccg ctgctggctg tacaaaaaac actaacaacc atcgcactgc
     2941 aacgacaagg agcgggacgg cgcggctcct cagtcgcccg accgggtgat cggcgtgact
     3001 tcgcgaatca gcaacagccc gtcgtacatc tcggccggta cgatcaactg ctcgttgagg
     3061 ccccactcga agctctttac gggctggcgg atccagggat gccgttccgc agccgtcccg
     3121 cgcaggtcga ggaacaccgg atcacccggc atcgctgcga ggcgcgcttc cagcgaatcg
     3181 gcctcgggcg ggcgcaggtc gtagtcctgg ttgatgaacc aggcatagcc gcgaccaccg
     3241 agtggataga tgccgagcgt gtagccggcg tcgccgagcg cgccctggac gaactcgccc
     3301 tggcgcggca cgccgccgac gaacctgctg taggcgtgcg cgatatgagc gttgtgcgcc
     3361 cagacgatga tgcgggcgtc cggctcgcgc tggatgtgcc gcagcagatt caccgccatg
     3421 cgctgctccc gcagccgttc gaacgccgcg cgatcgaact cggccggatt gaaatccagc
     3481 gtggccagcc ggtcctcgat gatcatcgcc agcatgggct ccgtcgtcgc ctcgcgcgca
     3541 gcctggaaac ccgccgcggc ccgccgccgg atttccgcca ggccctcctc accctcgaag
     3601 tcgccggcga aaatccgcag gaaactccgc tcggcggccg cgagcgtcgg cggcagtccg
     3661 ggcgttgcct cgatccagct cgagaaggcc cggctggtcg gactgttgtc gaagccgacc
     3721 agccgcatgg gccggccggc ggccgtcgcg ctgcgcaggt actcgaacag ggcctcggcc
     3781 tcctcgctgg cccaggcgcc ccagaagcag gcacgcatgc tttgctcggc agtccattcg
     3841 gccatgcgct gcgccgccac ccagcattcc gcgatgccgc cctccaccgc cagcacgtcg
     3901 aaaccatgcc gctcgtgcag gtgccgcacg atttcggtct tcagccggta ggtctcggcc
     3961 atgccgtgac cgctctcccc cagctgcagc aggcgccggt cgccgaccgc cgcggtgagt
     4021 gattgcagcc agggcggcgc ctcgccgccc ggcgtcgtcg cgggcggcga cgtggcgcag
     4081 cctgcgccga tgagagccgc cagccatacg acgcggataa ggcttttcgg catatgtcga
     4141 tcctcctctt gcgcatgcgc ctgcgccacc ggccgcggcg atggcgagat cgcccgagag
     4201 gctagggcct cacggtggat ccaggtcgtc ctcgcgcacc ggcgtgctgg ccgccacgtc
     4261 gcgggccagc cgccggccga gcacgcggtc gaggtgcttg ggcgccaggc cgaagcccgg
     4321 ccgcacgctg cgcaccgcgt ccgcggtgac ggtgtcgccg gccctgagcg ccttgacgaa
     4381 atacagcgag cggcggaact gtgcgttgcc ctgctcgctc gacttgcggc cgtaatcgac
     4441 ctggccgagc gtttcccacg cggtacgtgc gcccttgcag agcgcggcga gatcggcagg
     4501 ctcgagtgaa aaggtgtcgt ccggcccgcc gccgtgccgg tccagcgtga agtgcttctc
     4561 gatgagcgcc gcgccgaggg cgaccgccgc gatcgcggta gtgttgtcga gggtgtggtc
     4621 ggacaggccg gtcaccacgc cgaagcgatg ccgcatgtcc gccagggtac gcaggttgta
     4681 gtcggcggcc ggcgcaggat agccgctcac gcaatgcagg atcgccagct cgcggcagcc
     4741 gccgtcccgc gccgccgcga tagcctcgcc gatctcgtcc gcgtcggcca tgccggtcga
     4801 gatgatcatc ggcttgccgg tggcggccac gtagcggatc agtggcaggt ccaccgcctc
     4861 gaaggaggcg atcttgtagg ccggcgcatc gagcgactcc agcaggtcga ccgcagtggc
     4921 gtcgaagggg ctgctgaaga tcgtgatgcc ggccttgcgc gcatgtgcga acaagggcgc
     4981 gtgccattcc cagggcatgt gggcttcctg gtagagctcg tagagcgtgc ggccgtccca
     5041 caggccgccc ttgatcctga agtcctcggc gcccgaatcc agcgtgatgg tgtccggccg
     5101 gtaggtctgc accttgaccg cgtcggcgcc ggcgcgcttt gcggcatcga cgattgcgag
     5161 ggccgtctcg aggcggccgt ggtggttggc cgacatctcg gcgatgacgt acggcggcgc
     5221 atcggcggcg atgcgtcggc cggcgatgtg gatggacggc agggtcatgg cgtcgggtcc
     5281 ccgtggcggt ggcgcgaggg gccggccggg cgcgccccgg ccagccaggc tgcgcgctgc
     5341 aactggaagc atattaccgc ctgctcgccc tcgccatgct cgtccggctg ccgcctttcg
     5401 cccgcgcggt ggaagcccag cttttcgtgc atgcggatgg aaggggtgtt gaaggccagc
     5461 gcctcgcccc ggacttcgtc caccccgagc accccgaagg catgctcgag caccgcagcg
     5521 ccgagcgccg cgccggaacc gcgcggcgcc tccggagcca ggtaaaagcc ccaggccgcc
     5581 acgcggggcg ctgcgccgac ggccaggctg gcgaagccga gcggcgcacc gtcgcgctcg
     5641 aagatcagca ggtgccgcgc cgggtcggcg gcggcggcgg cgaaccaccg cgcgtgctcc
     5701 tcgggcgaga tttcgtggcg cgtgaacatg caggcgcgca cgtcgggatg attgcgccag
     5761 cgcagcaccc gctcgaggtc gtcggccgtc atgcggcgca cggcatcgcc cggtcccgtg
     5821 ctcacgacag cagtcgctcg gacacccgct cagcgccgcg cccgtcggtg atcccggcgg
     5881 cccgtcgggc cgcctgcgcc agcaccgccg gcgcgaccgc cgtggcgagc agtccgggca
     5941 gttcgcgcgg cactgcggcg acctcgtcga tcacccaggc cgcaccggcc cgcgccagcg
     6001 cctcggcgcc gggacgctgg ttgtccgcga gcaccaccac gatggacggc agcccgagac
     6061 agcagcgctc ccaggccgtg ccgccggccc cgccgatggc gaggtcggcc tgcagcatgc
     6121 gctcggccat gttgcgcacg tccaccagca cagtcgtcgg cagccgcatg tccgccgcgc
     6181 ggcgtcgcac cttgtccagc cagggcgagt cacgcccgct gatcaccgtc acggtcaccc
     6241 cgtccggcag cccggccgcc tcgataccgt ccagcacggc cgacgtcgcg tcgtccttgt
     6301 cgatgccgcc catggatacc agcaggttgc gcagcaacgg cgcttcgcgg cgcgcgaggc
     6361 tggctgcgcg caaggcccgg aactccgggc gcagcagcgc gaactgctgg ccgatgagcg
     6421 tctcgcagcc ggcaggcagc agcgccgcgt agtccgccgc gcgccgcccg aggttctggt
     6481 cgagcagcag gtcgcaggcg tgcggccggt cggcgaggtc gtcgatcacc agcgtccggc
     6541 cgcaggccgg cgccacgagc gcctcccagc gggcgtccag cgcgtaatga tccaccacca
     6601 gccagtccgg cggggcgtcc cgtagcgccg cgaggctcgc cgtcgcatcc tcggtccacg
     6661 gcccgccgag ccagtcggcg tgcggcggtc cgtcgggatc ggcctcgtcg tgctcggcgc
     6721 cgtccaccgg cagcagcagg accgcgtggc cccgcgcgcg gatgtgctcc gccaggtgtc
     6781 cctcatgggg ccgcgccagg aaccggcttt cgtggccacg cgccctcagg ctatcggcca
     6841 gcgtgaggca gcgcatgacg tggcccgagc cgatgcggat ggaggcgtcc gcgcgaaacg
     6901 cgaacctcat tccggctgcc cgcgttccgc ctgcatggcc cggaacagcc attcggcgcg
     6961 cagccagtcc tccggcgtgt cgatgtcctg cacgcggtgc cgcggcagga tcaccggcgc
     7021 cgcgccgtgg ccgaacaacg gcgcctcatc cagccaggcg gcggcacggc cccagtagaa
     7081 ctggccggcg tcgtgccagg cctcctcgag gtcctgggag cgcgtggcga actgcgccgg
     7141 atcgaacatt tccacccggc cctgcggcgt catccggagc gcccgctgga tggggaaggg
     7201 gaagctggtg acgctgaaag aatagtccgc gcccgactgc tcgagcgtct ccaggccgcg
     7261 ccgcaggtcc tccgcccgca ggaagggcgc cgtcgcgtag atgcagcaca cgcgcccggc
     7321 ctgccgatcc acggccaggg tctccacggc atggcgcatc accgccagcg tgccggcatg
     7381 gtcgtccgcc agcgccgcgg gacgcaggaa gggcacctcc gcgccgcacg cgcgggccgc
     7441 cgccgcgatc tgttcgtcgt ccgtggacac caccaagcga tcgaagcagc cgctctgcag
     7501 cgccgcctcg atcgaccagg caatgatcgg ccggccacag aactcgcgga tgttcttgcg
     7561 cgggatgcgc ttgctgccgc cgcgcgccgg gatgacggcc agcctcatcc ggccagcgcc
     7621 tc
//
LOCUS       DAOMPG010000110         7440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4269_length_7440_cov_3.520515, whole genome shotgun sequence.
ACCESSION   DAOMPG010000110 DAOMPG010000000
VERSION     DAOMPG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_7440_cov_3.520515"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLS34_07330"
     CDS             <1..553
                     /locus_tag="PLS34_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP87316.1"
                     /translation="SVADMQRLFESARGLGVGLRAHTGQLSDGGATAAACALGARSCD
                     HLEHADDSAVAAMAAHGTVAVLLPGAWYCLRDTVLPPVAALRAAGVPMAVASDANPGT
                     SPVVSLLTALHMAVVLFGLTPAEALAGVTRHAAQALGTAGGELAPGGPADFSAWPMDR
                     PALLGYQLGGLKPDAVFIGGERI"
     gene            550..2067
                     /gene="hutH"
                     /locus_tag="PLS34_07335"
     CDS             550..2067
                     /gene="hutH"
                     /locus_tag="PLS34_07335"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRP87317.1"
                     /translation="MSSSGGTRLTLGNGALTLAALRRWESDRGTVTLADAARARMDHS
                     VATVRRVIEAGQVSYGINTGFGAFANSRISAPQLTQLQYNLVRSHACGVGTPLPPELV
                     RRVLLLKANSLAVGLSGIRPEVVDALLSLLAADVLPIIPGRGSVGASGDLAPLAHLAL
                     ALIGEGEAMRGEGPEVLAAAGCDAVRLEAKEGLALLNGTQLSAALAIEGLFRAEALLG
                     TAITAGALSVEGLAGSYTPFDDRIHAARGLAPQRTVAARFRALLTDSDIHASHVSCDR
                     VQDPYAVRCMPQVFGAVAATLAHAREVLEAEANGVSDNPLVFDEDVLSGGNFHAAPVG
                     FVADFMAIAVAELASMSERRIDLLARRVNPNLKMFLTDEPGVESGFMIAHVTAAALAS
                     ENKALAHPASVDSIPTSAGQEDHVSMAPWAGHKLLTACDNAATVLAIELLAAAHAVDE
                     QRPLGTTPELATVHARIRAHAPFVRGDRRLDRDIAAIAALVRAGAFDAHERQFGS"
     gene            2190..2891
                     /locus_tag="PLS34_07340"
     CDS             2190..2891
                     /locus_tag="PLS34_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87318.1"
                     /translation="MPMFVDKESGFHHDHGQHFALTCSSCSVFAHMTPVAVPSFTDLS
                     RFKPPETGLVFRCDNCNAPVFLRFPVKAYTEERVELSTNFTEVERPKENFDFTYLPED
                     TEVLFREALDCYSMNALNAFASMCRRTVQRVFADLGENGKMRIFEQCNEIRDMAEVEG
                     ATFNVVRRVLFDTDASREGMPLVTPVEAGVLMEFMRDILYECYVRKGRLQQAMMMRRY
                     VAEQVEPPRQNGSHG"
     gene            complement(2888..4222)
                     /gene="pepQ"
                     /locus_tag="PLS34_07345"
     CDS             complement(2888..4222)
                     /gene="pepQ"
                     /locus_tag="PLS34_07345"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HRP87319.1"
                     /translation="MPSKADVLSLDALYPAHHAELVARYESVLAASPYDAIAVYSGVP
                     RMCFRDDQSWPFRAGAFYQQWVPCDEHAGCVLLFRPDRKPLLVLNLPDDYWHSVPEVP
                     AGPWTEHFDLEVASDPAGVRAALPKKLASCALIGEPDEQAAGWGCAALNPAAIVNPLE
                     FARLYKTAYEVECIAVANRIAARGHRAARDAFQAGASEFGIHLAYLEATGHSEAELPY
                     ANIIALNEHGATLHYGRFERAAPTPRRSFLIDAGARCRGYAADITRTWPAEPGGEFAA
                     LVDALDREQRALIDGLRPGASYVDVHEDAQLAVAVILEDFGIVAMAPDDMVAAGVSNV
                     FLPHGVGHHLGLQVHDTGGKWMSPDGTPLAQPEQWPFLRNLRPVEQGNVFTIEPGIYF
                     IGSLLERLRTTPLGRQVDWDAVDRLRPYGGARIEDNVWMGPGGARNLSREAF"
     gene            4419..5477
                     /locus_tag="PLS34_07350"
     CDS             4419..5477
                     /locus_tag="PLS34_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRP87320.1"
                     /translation="MSQSDVFAADLLETSASGYAALATERLLAEHPALAARFAPDAAG
                     TWKAALAQRIVELAAALRLGEPRLFVARISWARRAFAAREVPEDDLRASLEALAGVLT
                     RELPEAVGNSPATMLAAALAELDRPLAESDTRIDPRSATGRLTLEYLATVLEGDSRGA
                     TERVLAEVDAGLAVEAAYLEVLVPAQREVGRLWHAGELGIVEEHVVTYTTERLMALLA
                     HRAKRAPANGRTVVCAAVTGNAHDIAVRVLADFFDIAGWRTVHLGANVPVQELASALQ
                     YFDGDLLVLSASLSVQLPKLAEAIAAVRRLEGRTVRVMVGGLAFVDAPDLWRKLGADG
                     YAPDALTAVKRGAELVDA"
     gene            complement(5515..6396)
                     /locus_tag="PLS34_07355"
     CDS             complement(5515..6396)
                     /locus_tag="PLS34_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87321.1"
                     /translation="MILVAGASGELGRAICRQLVERGGTVYGMVRESSAPAAVAELEA
                     MGVTLVRADLASPESLREACSGCDSVVSGMTAMGRAGESVEKVDRDGQLALVAAAAEE
                     GVERFVYVSFSGVIGKDDPLTLAKRAVERTLKHSGMTWTVLRPSYFMESWFSPMLGFD
                     AAGGRARIYGAGREPISWVARDDVAAFAAQAVDSEEARNATLEIGGPEAIAPLDAVAL
                     FESVTGRSIEVETVDEEGLRAQLAAAREPQQRSLLALMLAYARGNAIPMEDMADRFGL
                     ELTPLRQWAERTLQAAG"
     gene            complement(6497..>7440)
                     /gene="hutU"
                     /locus_tag="PLS34_07360"
     CDS             complement(6497..>7440)
                     /gene="hutU"
                     /locus_tag="PLS34_07360"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRP87322.1"
                     /translation="VFEGIASRSIRPDLVTDQTSAHDPLNGYIPVGYDVAAAAEFRAA
                     DPAGYVRAAKDSMARQVRAMLALQAAGAPTFDYGNNIRARAQDAGVSNAFDFKGFVPL
                     YIRPLFCEGVGPFRWVALSGDPEDIWKTDAKARELFPDKTGLHRWLDGARERIRFQGL
                     PARICWLGYQERHRLGLAFNDMVASGELKGPIVIGRDHLDSGSVASPNRETEAMRDGS
                     DAVADWPVLNALLNTASGASWVSFHHGGGVGMGYSLHAGVVVVADGSARAAEQLARVL
                     VNDPGTGVMRHADAGYPRALEVAAERGLDLPMAPHGT"
BASE COUNT         1084 a         2674 c         2594 g         1088 t
ORIGIN      
        1 ctcggtcgcc gacatgcagc gcttgttcga gagcgcccgg gggctcggcg tcggcctgcg
       61 cgcgcacacc ggccagctgt ccgacggcgg cgccacggcg gccgcctgcg cgctgggcgc
      121 gcgcagctgc gatcacctcg agcatgccga cgactcggcc gtggccgcca tggcggcgca
      181 cggcacggta gcggtactcc tccccggcgc ctggtactgc ctgcgcgaca ccgtgctgcc
      241 gccggtggct gcgctgcgcg cggcgggcgt gccgatggcg gtggccagcg acgccaatcc
      301 cggcacttcg ccggtagtca gcctgctgac ggcgctgcac atggccgtgg tgctgttcgg
      361 gctgacaccc gccgaggcgc tggccggcgt gacgcggcac gcggcgcaag cgctcggcac
      421 ggccggtggt gaactcgcac cgggcgggcc ggcggacttc agcgcgtggc ccatggaccg
      481 gccggcactg ctcggctacc agctgggcgg cctgaagccg gacgcagtct tcatcggggg
      541 agaacggatt tgagcagtag cgggggaacc aggctgactc tgggcaacgg tgcgttgacg
      601 ctagcggcat tgcggcgatg ggagtcggac cgcggcacgg tgacgctggc cgatgcggcg
      661 cgcgcccgca tggaccacag cgtcgccacc gtgcgccgcg tgatcgaggc cggccaggtc
      721 agctacggca tcaacaccgg ctttggcgca tttgccaaca gccgcatttc cgcaccgcag
      781 ctgacgcaac tccagtacaa cctggtgcgc agccatgcct gcggcgtcgg cacgccgctg
      841 cccccggaac tggtgcgccg ggtcctgctg ctgaaggcca acagcctggc agtcggcctg
      901 tcgggcatcc ggccggaggt ggtcgatgcg ctgctgtccc tgctggcggc cgacgtgctg
      961 ccgatcatcc cggggcgcgg ctcagtgggc gcatccggcg atctcgcgcc gctggcgcac
     1021 ctggcgctgg ccctgatcgg cgaaggcgag gcaatgcggg gcgagggccc tgaggtgctg
     1081 gcggccgccg gctgcgacgc cgtccgcctc gaggccaagg aaggtctggc gttgctcaac
     1141 ggcacgcagc tcagcgcggc gctggccatc gagggactgt tccgcgccga ggcgctgctc
     1201 ggcacggcga tcacggccgg tgcgctcagc gtcgagggtc ttgcgggcag ttacaccccc
     1261 ttcgacgacc gtatccacgc cgcccgcggc ctggcgcccc agcgcacggt cgcagcgcgc
     1321 ttccgcgcgc tgctcaccga cagcgacatc cacgccagcc acgtgagctg cgatcgcgtg
     1381 caggacccct acgcggtgcg ctgcatgccg caggtgttcg gcgcggtcgc cgcgaccctc
     1441 gcgcacgcgc gcgaggtgct cgaggccgag gccaacggcg tgtcggacaa cccgctggtg
     1501 ttcgacgagg acgtgctttc gggcggcaat tttcacgccg ccccggtggg cttcgtcgcc
     1561 gacttcatgg ccatcgccgt ggccgaactc gccagcatgt cggagcgccg catcgacctg
     1621 ctggcgcgcc gggtcaaccc caacctcaag atgttcctga ccgacgagcc cggcgtcgag
     1681 tccggcttca tgatcgcgca cgttaccgcc gccgcgctcg cttccgagaa caaggcgctg
     1741 gcgcatcccg cctcggtgga ctcgatcccg acctccgccg gccaggagga tcacgtcagc
     1801 atggcgccat gggccgggca caagctgctc accgcctgcg acaacgccgc cactgtgctg
     1861 gccatcgagt tgctggccgc cgcgcacgcg gtggacgagc agcgtccgct gggcactacg
     1921 cccgaactgg cgacggtgca cgctcgcatt cgcgcgcacg cgcctttcgt gcgcggcgac
     1981 cggcggctcg accgcgacat cgccgcgatc gccgcgctgg tccgtgcggg ggccttcgac
     2041 gcgcacgagc ggcagttcgg gagctgacga agttttgctt ccaaggcatt gattttacat
     2101 aaccggactg ttggcgcggc tgccgcgcat tcggctacac tcgtctgcag aatgcgccgg
     2161 gcacaaggcg ccagggggga gcgggcgaga tgcccatgtt cgtcgacaag gagagcggtt
     2221 tccatcacga ccacggtcag catttcgcgc tgacctgttc ctcgtgcagc gtgttcgcgc
     2281 acatgacgcc ggtcgcggtg ccgagcttca ccgacctgtc gcgcttcaag ccgccggaaa
     2341 ccggcctggt gttccgctgc gacaactgca acgcgcccgt cttcctgcgc tttcccgtca
     2401 aggcctacac ggaggagcgc gtcgagttgt ccaccaactt caccgaggtc gagcggccga
     2461 aggaaaactt cgatttcacc tacctgcccg aggacaccga ggtgctgttc cgcgaggcgc
     2521 tggactgcta cagcatgaac gcgctcaacg ccttcgcgtc catgtgccgg cgcaccgtcc
     2581 agcgcgtgtt cgccgacctc ggcgaaaacg gcaagatgcg catcttcgag cagtgcaatg
     2641 agatccgcga catggcggag gtggaaggcg cgaccttcaa cgtcgtgcgg cgggtgctgt
     2701 tcgacaccga cgcctcgcgc gagggtatgc cgctggtcac gccggtcgag gccggcgtgc
     2761 tgatggagtt catgcgcgac attctctacg agtgctacgt gcgcaagggc cgcctgcagc
     2821 aggccatgat gatgcgccgc tatgtggcgg agcaggtcga gccgccgcgg cagaacggct
     2881 cacacggcta gaacgcctcg cggctgagat tgcgcgcccc gccaggcccc atccagacgt
     2941 tgtcctcgat gcgcgccccg ccgtagggcc gcagtcgatc caccgcatcc cagtcgacct
     3001 ggcggccgag cggcgtggtg cgcaggcgct ccagcagcga gccgatgaaa tagatgcccg
     3061 gctcgatggt gaacacgttg ccctgctcga ccgggcgcag gttgcgcagg aagggccact
     3121 gctccggctg cgccagcggc gtgccgtccg gagacatcca cttgccgccg gtgtcgtgca
     3181 cctgcaggcc caggtggtgg ccgacgccgt gcggcaggaa cacgttgctg acgcccgccg
     3241 cgaccatgtc gtcgggcgcc atcgccacga tgccgaaatc ctccaggatc accgcgacgg
     3301 ccagctgcgc gtcctcgtgc acgtccacgt aggaggcgcc gggacgcaga ccgtcgatga
     3361 gcgcgcgctg ctcgcggtcc agggcatcga ccagagcggc gaattcgccg cccggctccg
     3421 ccggccaggt gcgggtgatg tccgcggcgt agccgcggca gcgcgccccc gcgtcgatca
     3481 ggaagctgcg acgcggcgtc ggtgccgcgc gttcgaagcg tccgtagtgc aaggtagcgc
     3541 cgtgctcgtt gagcgcgatg atgttggcgt agggcagttc cgcttcgctg tgcccggttg
     3601 cctcgagata ggcgagatgg atgccgaact cgctggcgcc ggcctggaag gcgtcgcgcg
     3661 cggcgcgatg gccgcgggcc gcaatgcggt tggcgactgc gatgcactcg acctcgtagg
     3721 ccgtcttgta cagccgtgcg aactccagcg gattgacgat cgcggcggga ttcagcgcgg
     3781 cacagcccca cccggcggcc tgctcgtcag gctcgccgat gagcgcgcag ctcgcgagct
     3841 ttttcggcag cgccgcgcgc acgcccgccg gatcgctcgc cacctcgagg tcgaaatgct
     3901 ccgtccaggg gccggccggg acctccggca cggagtgcca gtagtcgtcg ggcaggttca
     3961 gcaccagcag cggcttgcgg tccggtcgaa acagcagcac gcagccggcg tgctcgtcgc
     4021 acggcaccca ctgctggtag aacgcccccg cgcggaaggg ccaggactgg tcgtcgcgga
     4081 agcacattcg cggcacgccc gaatacaccg caatggcgtc gtagggggaa gcggccagca
     4141 ccgactcgta gcgcgcgacc agctcggcat ggtgcgcggg gtacagggcg tcgagggaca
     4201 ggacatccgc cttgcttggc atcggaacac tccaggccgt ggatcgggcg ccgggggacg
     4261 gcgacggctt cgaaggtccg attctatcgc ccgcgccgct ataatcgaat ccacgcgggg
     4321 ttctgccgtc gaatccggcc cctgagcatc gcatttcaag ctgcgatgcg ccgcaccgcc
     4381 agccaggctg gcggcggtcc tgtcaacgga ggcaacgcat gagccagtcc gacgtgttcg
     4441 ccgccgacct gctggagacc agcgcctccg gctacgcggc gctggccacc gagcggctgc
     4501 tggcggagca cccggcgctg gcggcgcggt ttgcaccgga cgcggcgggc acctggaagg
     4561 ccgccctcgc ccagcgcatc gtcgaactgg ctgctgcgct gcggctgggt gagccccggt
     4621 tgttcgtggc ccgcatcagc tgggcacggc gcgccttcgc cgcacgggaa gttcccgagg
     4681 acgacctgcg tgccagcctc gaggcactgg ccggggtctt gacccgggaa ctgccggagg
     4741 ccgtcgggaa cagccccgcc accatgctgg cggccgcgct ggccgagctc gatcgcccgc
     4801 tcgccgaaag cgacacccgt atcgacccgc gttccgccac cgggcggctg acgctcgagt
     4861 atctcgccac cgtgctcgag ggcgacagcc gcggggcgac cgagcgcgtg ctggccgagg
     4921 tggacgcggg gctcgccgtc gaagccgcct atctcgaggt gctggtgccg gcccagcggg
     4981 aggtcggccg actgtggcat gcgggcgagc tcggcatcgt cgaggagcac gtcgtcacct
     5041 acaccaccga gcggctcatg gcgctgctgg cgcaccgcgc gaagcgcgcg ccggccaacg
     5101 gccgcaccgt ggtgtgcgcg gcggtcaccg gcaacgcgca cgacatcgcc gtgcgcgtgc
     5161 tggccgactt cttcgacatc gccggctggc gcacggtgca cctgggcgcg aacgtgccgg
     5221 tgcaggaact ggcgtcggcg ttgcagtact tcgacggcga cctgctggtg ctgtcggcct
     5281 cgctcagcgt gcagctgccg aagctggccg aggcgatcgc ggccgtgcgg cgcctcgagg
     5341 gacgcacggt gcgcgtgatg gtcggcgggc tggccttcgt cgacgcaccc gatctctggc
     5401 ggaagctggg tgccgacggc tacgcgcccg atgcgctgac cgccgtcaag cgcggcgcgg
     5461 agctggtcga cgcctgaacc ggcgccgcac gcgcacgcgg cggcggcacg tgtctcaacc
     5521 ggcagcttgc agcgtgcgct ccgcccattg ccgcaggggc gtgagctcca gcccgaagcg
     5581 atccgccatg tcctccatgg ggatcgcgtt gccgcgcgca taggccagca tcaacgccag
     5641 gaggctgcgc tgctgcggct ccctggccgc cgccaactgc gcgcgcaggc cctcctcgtc
     5701 caccgtttcg acctcgatcg accggccggt gacggactcg aacagcgcca ccgcgtcaag
     5761 cggcgcaatg gcctccggtc cgccgatctc cagtgtggcg ttgcgggcct cttcgctgtc
     5821 caccgcctgg gcagcaaagg cggcgacgtc gtcgcgcgcc acccagctga tgggctcccg
     5881 accggcgccg taaatgcgcg cccggccgcc cgccgcgtcg aaaccgagca tgggcgagaa
     5941 ccaggactcc atgaaatagg agggccgcaa cacggtccag gtcatgccgc tgtgcttgag
     6001 cgtgcgttcc accgcgcgct tggcgagcgt cagcgggtcg tccttgccga tgacgccgga
     6061 gaacgagacg tagacgaaac gctccacgcc ctcctcggcc gccgccgcca ccagcgccag
     6121 ctgcccgtcg cgatcgactt tctcgacgct ctcgcccgcg cgccccatcg ccgtcatgcc
     6181 cgacaccacc gagtcacagc cgctgcaggc ttcgcgcaac gactccggac tggcgagatc
     6241 ggcgcgcacc agcgtcacgc ccatggcctc cagctccgcg acggcggccg gcgcggagct
     6301 ctcgcgcacc atgccgtaca ccgtcccccc gcgctccacg agctgacggc aaatggcccg
     6361 ccccagctcg cccgacgccc cggcgaccag gatcatgttc cttctcctcg ctccgtccca
     6421 gatgctttct tgtcgtcgcc gccgacgccc gcggacgccg gcaatgactg aatttcccga
     6481 atcacgcggt agtgcgtcaa gtcccgtgcg gcgccatggg caggtcgagg ccgcgctcgg
     6541 cagccacttc gagggcccgc gggtagcccg cgtcggcatg ccgcatcacg ccggtgcccg
     6601 gatcgttgac cagcacgcgg gccagctgtt cggccgcccg cgcgctgccg tcggccacca
     6661 ccaccacccc ggcgtgcagc gaataaccca tgccgacgcc gccgccgtgg tgaaagctca
     6721 cccacgacgc gccgctggcc gtgttgagca aggcattcag caccggccag tccgccaccg
     6781 cgtcggagcc gtcgcgcatc gcttcggtct cgcggttggg cgaggcgacc gagcccgaat
     6841 cgaggtgatc gcggccgatg acgatcggcc ccttgagctc gccgctcgcc accatgtcgt
     6901 tgaaagccag cccgagacga tggcgttcct ggtagccgag ccagcagatg cgcgccggca
     6961 gcccctggaa gcggatacgc tcccgggcac cgtccagcca gcggtgcagg ccggtcttgt
     7021 ccggaaacag ctcgcgggcc ttggcgtcgg tcttccagat gtcctctggg tcgccggaca
     7081 gcgcgaccca gcgaaacggc ccgacgccct cgcagaacag cggccggatg tacagcggca
     7141 cgaagccctt gaagtcgaag gcgttgctga caccggcgtc ctgcgccctg gcgcgaatgt
     7201 tgttgccgta atcgaaggtc ggcgcgccgg cggcctgcag cgcgagcatg gcgcgcacct
     7261 gccgggccat ggagtccttg gcggcgcgca cgtagccggc cggatccgcg gcacggaatt
     7321 ccgccgcggc cgccacgtca tagccgaccg ggatgtagcc gttcagcgga tcgtgcgcgg
     7381 aggtctggtc ggtgaccagg tcgggccgga tgctccgcga cgcgattccc tcgaacactt
//
LOCUS       DAOMPG010000111         7383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4306_length_7383_cov_4.924400, whole genome shotgun sequence.
ACCESSION   DAOMPG010000111 DAOMPG010000000
VERSION     DAOMPG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4306_length_7383_cov_4.924400"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLS34_07365"
     CDS             <1..301
                     /locus_tag="PLS34_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87323.1"
                     /translation="EEMRYGSEEECKALIGKIRNAVAVLGPSRPSLADIETRAQSITR
                     QAQFRSARETVPMAASVSRIDLPVRSSEGPSVRRIPLEVNVLAEEYWDIYDVFIP"
     gene            complement(291..3380)
                     /locus_tag="PLS34_07370"
     CDS             complement(291..3380)
                     /locus_tag="PLS34_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87324.1"
                     /translation="MNLTQGAISRPVTTVMIFIALALIGAISSRLLPLEKYPDIQFPG
                     IFISIPYEGSTPEEIEQLITRPVEEALATLSGVARMQSTSRQDNSQIFLQFGWDQDVA
                     TKGIEARAKVDAIRHLLPDDVRRVLIFTGSTSDQPILNLRISSDRDLSDAYTMLDRQL
                     KRRIERIEGVSRVTLYGVDQHEIRILLNADRVAAHGVDLNQLRELLEKANFSVSAGRL
                     TDNQQRFSVRPIGEMSSVEDVRNILVRENLRLSDIADVELRSPERTHGRHLDRKYSIG
                     LDVFKQPGANLVDVSRRVKQEVERAGENPMMSGIQVFALGDQADGVTSSLSDLLSSGL
                     IGALLAFAVLYLFLRQAATTLIVVACVPFSILITLGVMYFLGVSLNILSMMGLMLAIG
                     MLVDNAVVVTESIFRERQLDPDNPRQATLKGVREVALAVTAGTLTSIIVFVPILFGVK
                     TNITVFLTHVAVSIVVAMLASLVMAQTVVPMVAARIPAPRAPRASSWMSRLTGRYVRA
                     LGWTLRHRWWTALCVLLVVASVAIPLKAVKFDTFPQEAGRLLLLQYHVKEKYPMAETK
                     RAVEIMEEYLYSNQEYFDIRQVYSFYEEGRAQSTLMLNDGDDARVPTSEVIARVLENM
                     PEIIIGEPSFEFETQGGDEGFSLQLAGESTEVLARLAPEVVRMLGSIEGLRDIKADIG
                     SGDREVRVKVDRERAIQLGLSPQSVATSVSVALRGEQLRDFRSGDGEVKVRIAFREDD
                     RQSIEQLAALPLYTPDGQRVTLGTIADFEVTQAFQEIRRMDRVTAVVINANLDGRSMD
                     QVRPEITALMDAYQLPPGYSWKFGRGFQQADETQQVMAQNILLAIALIFLVMAALFES
                     TVYPLSIITSIGFSIIGVFWFFMITGTTFSFMATIGIMILVGVVVNNGIVLIDHVNNL
                     RRDGLERDAALLQAGRDRLRPILMTVATTILGLLPLAVSATKVGGDEIGAPAYFPMAR
                     AIIGGLGFSTLTSLLLVPWTYALLDDLGRWGRRVKAVARRSAGGGAAATARANPLRE"
     gene            complement(3377..6583)
                     /locus_tag="PLS34_07375"
     CDS             complement(3377..6583)
                     /locus_tag="PLS34_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008844440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87325.1"
                     /translation="MRLIDIATERRVTIAMFTVAIVLFGFVSLSRLKVNLLPDLSYPT
                     LTVRTELPGTAPLEVENLLSKPIEESVGIIKNVRQVRSVSRPGQSDVILEFLWGTDMD
                     IAGVEVREKLDMLQLPLEASRPLLLRFDPANEPIQRLAFMDKSATGAADDPNRLRALR
                     TFAEDRLRTDLESVEGVAAVKVSGGLEDEIQILIDQEKTAQLGLSIRAIAARLRAENV
                     NLSGGRLEQGAQRFLVRTLNEFVTVQEMADSIIATPAGRPIYLRDIATVTRGFKEREA
                     ITRVNGRESVELAVYKEGDANTVQVAHRVAQRIAAMQARLEPGTELTMIYDQSVFIDD
                     AITQVRSAALVGGLLAILVLYAFLRDARATFVIGVAIPISVIGTFLLMYAAGVSLNIM
                     SLGGIALAVGMLVDNSIVVLENIVRNKEQGHGVLEAARRGASEVAGAVTAATLTTVAV
                     FFPMVFVSGIAGQLFRDQALTVTFALLFSLLVALTLIPMLASRGAPPRYGDAAHAAPP
                     GRVTRAAGFVVRLLGYATAGVSWLMSRVMRPLVWIFQGFYRAAAAIYLPLLERSLRQR
                     WLVLLVAALAFASSLALAPRLGYELLPQLAQGEFAVEVRLPAGAPLAETDAAIRRLQE
                     QSADLPGIALTYSVAGTGNRLDASPVDAGEHTGQLNVKLKPGAGPTGEAATVASMRAT
                     LERMPGIAYEFSRPALFAVSTPLEIEIAGYDLDQIGSLAEQVRRALGASPRFADVRTS
                     VEEGNPEIQILFDQERAARLGLVVRDIADIVVAKVRGEVATRYTWRDKKIDVLVRSID
                     TRASSLTEIENLIVNPESSRPIPLKAVAEIQLALGPAEIRRSDQSRVAIVSANLAFGD
                     LATATEEVREILAGLPFSPGTAASIAGQSAEMQDSFQSMMFTLALAIFLVYLVMASQF
                     ESLIHPFVILFTIPLALVGAVWALWITGTTVSVVAFIGLIMLAGIVVNNAIVLVDLIN
                     QLRRAGQELETAIIEAGRARLRPILMTTLTTVLGLLPMALGFGEGSEVRAPMAITVIG
                     GLVVSTLLTLLVIPVVYSLFDRGMQAVSNARLAAEATG"
     gene            complement(6608..>7383)
                     /locus_tag="PLS34_07380"
     CDS             complement(6608..>7383)
                     /locus_tag="PLS34_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP87326.1"
                     /translation="ERARANLRKLEQEYNRSVELFERGLVSSGAFEGQKYELEALQAI
                     YRLAQLEYNYTQVRAPIAGVVTRRYIRTGNTISVNEPAFQVTELDPLIAYIHIPEREF
                     RRLEPGQSAELTLDAIPGQRFAAHVQRISPVVDPISGTFKVTMEVPDQGGRLKPGMFG
                     RFNIVWDTRRDALLAPRVAIVDDDVSDSVFVVVDGKAVRRPVRTGYARGAQVEIIDGL
                     NGDEEIVVIGQSGLRDGANVEVVRRAGTGAEPRVARDGA"
BASE COUNT         1257 a         2407 c         2536 g         1183 t
ORIGIN      
        1 cgaggaaatg cgctacggca gcgaagaaga gtgcaaggcg ctgatcggca agatccgtaa
       61 cgcagtcgcg gtgctcgggc cgagccgtcc gagccttgcg gacatcgaga cgcgcgccca
      121 gtcgatcacc aggcaggccc agttccgcag cgcccgcgag accgtcccga tggccgcgtc
      181 ggtgtcacgc atcgacctgc cggtgcgctc aagcgagggc ccgtcggtgc gccgcattcc
      241 gctcgaggtc aacgtgctgg cggaagagta ttgggacatc tacgacgtgt tcattccctg
      301 agcgggttcg cgcgcgcggt cgccgcggcg ccgccgcccg cggagcggcg cgccactgcc
      361 ttgacacgac gaccccagcg gccgaggtcg tcgagcaacg cgtaggtcca gggtaccagc
      421 agcaatgacg tcagcgtcga gaacccgagg ccaccgatga tggcgcgcgc catcgggaag
      481 tacgccggcg caccgatctc gtcgccgccc accttggtgg cgctgacggc cagcgggagc
      541 aggcccagaa tggtagtcgc gacggtcatg aggatgggac gcagacggtc gcggcccgcc
      601 tgcagcagtg ccgcatcgcg ctccagcccg tcgcgccgca ggttgttgac gtggtcgatc
      661 agcacgatac cgttgttcac caccacgccc accaggatca tgattccgat ggtggccatg
      721 aacgagaagg tcgtgccagt gatcatgaag aaccagaaca cgccgatgat cgagaagccg
      781 atcgaggtga tgatcgacag gggatagacg gtggattcga acagcgcagc catcacgagg
      841 aaaatcaatg cgatggccag caggatgttc tgcgccatga cctgctgcgt ctcgtccgcc
      901 tgctggaagc cgcgcccgaa tttccagctg tagcccggcg gcagctggta ggcgtccatc
      961 agggccgtga tttccggccg cacctggtcc attgaacggc cgtcgaggtt ggcgttgatg
     1021 accactgccg tcacgcggtc catgcgccgg atttcctgga aggcttgggt aacctcgaag
     1081 tcggcaatgg tgcccagcgt cacgcgctgg ccgtccggcg tgtagagggg cagggcagcc
     1141 agctgctcga tgctctggcg gtcgtcctcg cggaacgcga tgcgcacctt gacttcgccg
     1201 tcgccgctgc ggaagtcgcg caactgctcg ccgcgcagtg cgaccgacac ggacgtggcg
     1261 acggactgcg ggctcaggcc gagctggatg gcgcgttcgc ggtcgacctt cacgcgcacc
     1321 tcgcggtcgc ccgagccgat gtcggccttg atgtcgcgca agccctcgat gctgccgagc
     1381 atgcgcacga cttccggtgc gaggcgggcc agcacctcgg tcgactcgcc ggccagctgc
     1441 aggctgaagc cctcgtcgcc gccctgggtc tcgaactcga agctcggctc gccgatgatg
     1501 atctccggca tgttctccag aacgcgtgca atgacttcgc tggtaggcac gcgggcgtcg
     1561 tcgccgtcgt tcagcatcag ggtgctctgc gcgcggccct cctcgtagaa gctgtacacc
     1621 tgccggatgt cgaagtactc ctggttggag tacaggtact cctccatgat ttccacggcc
     1681 cgcttggtct cggccatggg gtacttttcc ttcacgtggt actgcagcag cagcaggcgt
     1741 ccggcctcct gcggaaaggt gtcgaacttg accgctttca gcgggatcgc gacgctggcg
     1801 acgaccagga gcacgcacag cgcggtccac cagcggtgtc gcaaggtcca gcccagggca
     1861 cgcacgtagc ggccggtcag gcggctcatc caggaactgg cccgcggcgc gcgcggggcc
     1921 gggatgcggg ccgcgaccat cggcacgacg gtctgtgcca tgaccagcga ggcgagcatc
     1981 gccaccacga tgctcacggc gacgtgcgtc aggaacaccg taatgttggt cttgacgccg
     2041 aacaggatcg gcacgaacac gatgatggaa gtgagcgtac cggcggtcac cgcgagggcg
     2101 acttcccgga cgcccttgag ggtcgcctgg cggggattgt ccggatcaag ttgccgctcg
     2161 cgaaaaatac tctcggtcac caccaccgcg ttgtcgacca gcatgccgat ggcgagcatc
     2221 aggcccatca tcgacaggat gttcaggctc acgcccagga agtacatcac tccaagagtg
     2281 atgagaatcg agaagggcac gcacgccacc acgatcagcg tggtggccgc ctggcgcagg
     2341 aacaggtaga gcacggcgaa ggccagcagc gcgccgatca ggccggagct caacaggtcg
     2401 gacagcgaag acgtcacacc gtccgcctgg tcgcccagcg cgaacacctg gatgccgctc
     2461 atcatcgggt tctcgccggc gcgctcgacc tcctgcttga cgcgccggga cacgtcgacc
     2521 aggttggcgc ccggctgctt gaagacgtcc agcccgatgg agtacttgcg gtcgagatgc
     2581 cggccatgag tccgctcggg gctgcgcagc tcgacgtcgg cgatatcgct gaggcgcagg
     2641 ttctcgcgca ccaggatgtt gcgcacgtcc tccaccgagg acatttcgcc gatgggccgt
     2701 acgctgaagc gctgctggtt gtcggtcaat cgcccggcgc tgaccgagaa attcgccttt
     2761 tcgaggagtt cgcgcagctg gttgaggtcg acgccgtggg cggcgacccg gtcggcgttc
     2821 agcaggatgc ggatctcgtg ctggtcgacg ccgtacaggg tgacccgtga caccccctcg
     2881 atgcgctcga tgcgccgctt gagctggcga tcgagcatgg tgtaggcgtc ggacaggtcc
     2941 cggtcgctgc tgatgcgcag gttgagaatc ggctggtcgc tggtcgagcc ggtgaaaatg
     3001 agtacccggc gcacgtcgtc cggcagcagg tgccggatcg cgtccacctt ggcgcgcgcc
     3061 tcgatgccct tggtggccac gtcctggtcc cagccgaact gcaggaagat ctggctgttg
     3121 tcctgccgcg aggtggactg catgcgggcc acgccgctca gtgtggcgag cgcttcctcg
     3181 accggccgcg tgatgagttg ctcgatctcc tcgggggtag agccttcgta ggggatgctg
     3241 atgaatatgc cgggaaactg gatgtcgggg tatttttcca gcggcagcag gcgcgacgag
     3301 atagcgccga tgagcgccag cgcaatgaat atcatcacgg tggtgaccgg cctgctgatc
     3361 gcaccctgcg tgagattcat ccggtggcct ccgcggccag gcgggcattg ctgacggcct
     3421 gcatgccgcg gtcgaacagg gagtaaacca ccgggatcac cagcagtgta agcagtgtgg
     3481 acacgaccag cccgccgatc acggtgatgg ccatcggcgc gcgcacctcc gagccctcgc
     3541 cgaagcccag cgccatcggc agcaggccca gcacagtggt cagggtggtc atcaggatcg
     3601 gtcgcagtcg cgcgcgtccg gcctcaatga tcgccgtttc gagctcctgg ccggcacgcc
     3661 gcagctggtt gatgagatcg accagcacga tggcgttgtt gaccacgatg cctgccagca
     3721 tgatcaggcc gatgaaggca acgacgctca cggtcgtgcc ggtgatccac aaggcccaga
     3781 cggcgccgac cagcgccagc ggaatggtga acaggatgac gaagggatga atcagcgact
     3841 cgaactgtga ggccatcacc aggtacacga ggaatatcgc cagggcgagc gtgaacatca
     3901 tggactggaa cgagtcctgc atctcggcgc tctggccggc gatgctggcg gccgtgcccg
     3961 gactgaacgg gagcccggcg aggatctcgc ggacttcctc cgtggcggtc gccaggtcgc
     4021 cgaacgccag gttcgcggac acgatggcga cgcgcgactg gtccgagcgg cggatttcag
     4081 cagggccgag cgccagctgg atttcggcca cggccttcag cggtatcgga cggctgctct
     4141 cggggttgac gatgagattc tcgatctccg tgagcgagga tgcacgggta tcgatactgc
     4201 gcaccagcac gtcgatcttc ttgtcgcgcc aggtgtaacg cgtggccacc tcgccgcgca
     4261 ccttggcgac gactatgtcc gcgatgtcgc gtaccaccag gccgaggcgg gctgcccgct
     4321 cctggtcgaa caggatctgg atctccgggt tgccttcctc gacgctggtg cgcacgtcgg
     4381 cgaaacgcgg cgacgccccc agcgcccgcc gcacctgctc tgcaaggctg ccgatctgat
     4441 cgaggtcgta gcccgcaatc tcgatctcca gcggcgtgct gacggcgaac agggccgggc
     4501 gggaaaactc gtatgcgatg ccgggcatac gctccagtgt ggcgcgcatg gacgcaacgg
     4561 tagccgcttc gccggtcggt ccggcgcccg gcttgagctt cacgttgagt tgcccggtat
     4621 gctcgccggc gtccaccggg ctggcgtcca ggcgattgcc ggtgccggcc acggagtagg
     4681 tcagtgcgat gcccggcaga tcagcgcttt gctcctgtaa gcgacggatg gcggcgtcgg
     4741 tctcggcgag cggcgcaccg gcgggcagtc ggacctcgac tgcgaattcg ccctgtgcca
     4801 gctgcggcag cagctcgtag ccgagccgcg gcgccaatgc caggctcgag gcaaaggcga
     4861 gggcggcgac cagcagcacc agccagcgtt gccgcagcga ccgctccagc aacggcaggt
     4921 agattgcggc tgcggcccgg tagaagccct ggaaaatcca taccagcggg cgcatcaccc
     4981 gcgacataag ccaggacacg ccggccgtcg catagccgag caggcgcacg acgaagcctg
     5041 ccgcgcgggt cacgcgtccg ggtggggcgg catgcgcggc gtcgccgtaa cggggcggtg
     5101 cgccgcggga cgccagcatg ggaatcagcg tcagggcgac cagcagtgag aacaacagcg
     5161 caaaagtaac cgtcagcgcc tggtcgcgaa acagctgccc cgcgatgccg ctgacgaaca
     5221 ccatggggaa gaacaccgct acggtcgtga gcgtcgcggc ggtgactgca ccggcgacct
     5281 cgctggcccc gcgcctggcg gcttccagca cgccgtgacc ctgctccttg ttgcgcacga
     5341 tattttcgag caccacgatg ctgttgtcga ccagcatgcc gaccgccagc gcgatgccgc
     5401 cgagcgacat gatgttcagg ctcacgcccg ccgcgtacat cagcaggaag gtgccgatca
     5461 ccgagatggg aatggcgacg ccgatgacga aggtggcgcg ggcgtcgcgc aggaaagcgt
     5521 acagcacgag gatggcgagc aggccgccga ccagggcggc gctgcggacc tgggtgatgg
     5581 cgtcgtcgat gaacaccgac tggtcgtaga tcatggtcag ctcggtgccc ggctcgagcc
     5641 gcgcctgcat ggcggcgatc cgctgggcga cccggtgcgc cacctgcacg gtgttggcgt
     5701 caccctcctt gtagaccgcc agctcgacgc tctcgcggcc gttgacgcgt gtgatcgcct
     5761 cgcgttcctt gaagccgcgc gtgacggtgg cgatatcccg caggtagatg gggcggccgg
     5821 ccggcgtggc gatgatggaa tccgccatct cctggacagt gacgaattcg ttcagcgtgc
     5881 gcaccaggaa tcgctgcgcc ccctgctcga gccggccgcc ggacaggttg acgttctcgg
     5941 cccgcaggcg ggccgcgatg gcgcggatcg acaggccgag ctgggcggtc ttctcctggt
     6001 cgatcaggat ctggatctcg tcctccaggc cgccgctgac cttgacggca gccacgcctt
     6061 cgaccgactc gaggtcggtc ctcaggcggt cctccgcgaa agtccgcaac gcgcgcaggc
     6121 ggttcgggtc gtccgcggcc ccggtggcgg acttgtccat gaaggcgagc cgctggatgg
     6181 gctcgttggc cggatcgaaa cggagcagca gcggccggct cgcttccagc ggcagctgca
     6241 gcatgtccag cttctcgcgc acttcgaccc cggcgatgtc catgtcggtg ccccagagaa
     6301 actccaggat cacgtcggac tggccggggc gggacaccga gcgcacctgg cgcacgttct
     6361 tgatgatgcc gaccgactcc tcgatcggct tgctcagcag gttctcgacc tccagcggcg
     6421 cggtgccggg cagctcggtg cgcaccgtca gagtggggta ggagagatcg ggcagcaggt
     6481 tgaccttgag gcgcgacagg gaaacgaagc cgaacagcac gatggcgacg gtgaacatcg
     6541 cgatggtgac gcgtcgctcg gtggcgatgt cgatcaggcg catgacagtg ctccgccggg
     6601 cgccgcgtca ggcgccgtca cgggcgacgc ggggctcggc accggtcccg gcgcgccgca
     6661 cgacctcgac gttggccccg tcgcgcaatc cggactgccc tatgaccacg atctcctcgt
     6721 cgccgttgag tccgtcgatg atctccactt gcgcaccgcg ggcataaccg gtgcggaccg
     6781 ggcgccgcac ggccttgccg tccacgacca cgaacaccga gtcggacacg tcgtcgtcca
     6841 cgatggcgac ccgcggtgcc agcaaggcat cccgccgcgt gtcccagacg atgttgaagc
     6901 gaccgaacat cccgggcttc aggcggccgc cctgatcggg tacctccatg gtgaccttga
     6961 aggtgccgga gatcggatcc acgaccgggc tgatgcgctg cacgtgggcc gcgaagcgct
     7021 ggccgggaat ggcgtccagg gtcagctccg cggactgccc gggctcgagt cgccggaact
     7081 cgcgctccgg aatgtgaatg taggcgatca gggggtccag ctcggtgacc tggaaggccg
     7141 gctcgttcac cgagatggtg ttgcccgtgc ggatgtaacg gcgcgtgacg acaccggcga
     7201 tcggcgcccg gacctgcgtg tagttgtact ccagctgggc cagccggtag atggcctgca
     7261 aggcctcgag ctcgtatttc tggccctcga aggcgcccga gctgaccagg ccgcgctcga
     7321 acaactccac gctgcggttg tattcctgct ccagcttgcg caggttggcg cgcgcccgct
     7381 cca
//
LOCUS       DAOMPG010000112         7072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4555_length_7072_cov_5.209491, whole genome shotgun sequence.
ACCESSION   DAOMPG010000112 DAOMPG010000000
VERSION     DAOMPG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_7072_cov_5.209491"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLS34_07385"
     CDS             complement(<1..264)
                     /locus_tag="PLS34_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRP87327.1"
                     /translation="MLRARQKLGKYRIERRLSDGPLAAVYQAFDTIHGQRVALKIPHR
                     RMMDDYFLADFRKEVRMLAKLDHPNILPIQNACFIDGYFVISMP"
     gene            468..680
                     /locus_tag="PLS34_07390"
     CDS             468..680
                     /locus_tag="PLS34_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87328.1"
                     /translation="MTGYERKDFSDEADFEFTGDAEPMDLLPDIERRETRRSGPGTAA
                     RRTLEALAESRRLSRELAELDHFEDI"
     gene            788..1375
                     /locus_tag="PLS34_07395"
     CDS             788..1375
                     /locus_tag="PLS34_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRP87329.1"
                     /translation="MNAVTARSGLAAAAMLALAACTTAGPEIRTDYDAATDFSQFSTF
                     AFMDRAERGETRTYDTIGDRRVMSSVQRELEARGYRRVDSDPDLLVNFAMATEDVQDV
                     RSVPSSIVPPPWYGWRGGYYYPWPTYAYETYVDSYQRGTLYIDLVDAERRQLVWEGRA
                     VGRVTRATREDPAGALDAAVVEIFAQYPFRAGPLR"
     gene            complement(1398..1835)
                     /locus_tag="PLS34_07400"
     CDS             complement(1398..1835)
                     /locus_tag="PLS34_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87330.1"
                     /translation="MPTRNRRCRITCLPAAALLLAGCAPTQPWWQQDLQEWQGAPVSE
                     LLAAWGAPLRTLTGEDTATVLVFERFRELDRRLESLADPAARLDPERDRTALVVPERS
                     ECTFYFEILADRVVAARHEGAACDVVARDPARRRADPEPTRRR"
     gene            complement(1846..3129)
                     /locus_tag="PLS34_07405"
     CDS             complement(1846..3129)
                     /locus_tag="PLS34_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP87331.1"
                     /translation="MLHSTLFRRLAALLTLLVGSTALAADLEWFTTGPDGESRVVVYY
                     FYSDTCPHCIEAKPFVASLDELGWIELHAHEVTKNRDNALLYRDVAAAAGEQARSVPA
                     FIFCGSMHTGFERPETTGTVLLARMEHCRTNPELVLGAQSGGAPEPGKVMGVEVPVLG
                     LINPSDWGLPILTVIIAGLDAFNPCAFFVLLFLLSLMVHARSRARMLLVGGTFVVISG
                     VIYFVFMAAWLNVFLLIGELRMVTLIAGLVAVTLAAINIKDYFWFDAGGPSLSIPESK
                     KPGMYQRMRNLVAAKSIHAMLAGTAVLAVAVNSYELLCTAGLPMVYTRILTLREADTF
                     VYYMYLVLYNIIYVIPLLVIVLLFVMKFGSRKLGEREGRLLKLMSGMMMLGLGAVMLF
                     APTALNNAGIAIGLLGVAVLGTWIIHAVMKKRGLI"
     gene            3263..3499
                     /locus_tag="PLS34_07410"
     CDS             3263..3499
                     /locus_tag="PLS34_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRP87332.1"
                     /translation="MREIIDQSVRHARRLIVAVVGVTVLLFGLALVILPGPAFVVIPL
                     GLAILAAEFAWARSLLRRVRRGISNGLRNGRLNR"
     gene            complement(3511..4935)
                     /locus_tag="PLS34_07415"
     CDS             complement(3511..4935)
                     /locus_tag="PLS34_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRP87333.1"
                     /translation="MSRHFFASLTQNPISLVGTALAAASLTLIVALFIVQMVGFEGGP
                     YLGILTFLVLPMFFVLGLLLIPFGIMRQRKRAARAAAAGEAPPLFPIIDLNQETTRKS
                     VIVFMVLTMVNIIVLAGATYKGVEIMDSTEFCGMACHKVMQPEHTAHQRSPHSRVGCV
                     DCHIGPGADWFVKSKLDGAWQLVAVALNIYPRPIETPLHNLRPARDTCEQCHWPTKFV
                     GDKLKIDTVYNEDELNTELRSAVLLKVGGLHGRESSGIHWHVDPNIQIRYLSDPDRMN
                     IYDVEMTKADGSVKVFLAGEAPADAEWRVMDCVDCHNRPTHIYRAPAEEIDLLLAAGK
                     IDTGLPYVKREGVRIIEEANYETHDAARVGITESLNAFYRENYPEVLAQNAVAVTAAA
                     DALGDVYSWNVFPHMKVRWDTYPNHSGHPPVRDRQDAPGCFRCHNRQHQTAEGERISR
                     SCDLCHTVLAQREEDPEILRLLNP"
     gene            complement(5053..7029)
                     /locus_tag="PLS34_07420"
     CDS             complement(5053..7029)
                     /locus_tag="PLS34_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87334.1"
                     /translation="MARHTSFPALLRGLLAVAALSLAATAVNAQEREINCMACHDDVH
                     FDSTAHAEVACKDCHTNVTRPRHKKEDLADLTGDAICAQCHSMATRAMGRSVHGDKVA
                     CVECHGDAHVMRKNDDLLSPMSPVGQVQTCGDCHAQLPGQKHMIDSFVDSVHGRGLLR
                     SGLVSSPTCSTCHGSHRVVEVENERSPTSFQNSPQMCGDCHQYVYQEWLNVSAHGVAW
                     TAGNTGTATCIDCHSSHEIRDPVTDRAERLGIVDQCGDCHGDYYQLYRGDFHGKATNL
                     GLAVSATCADCHHPHATLGKDDPRSTIHTDNLQATCGDCHGAVTAGFASYNPHIDSSD
                     PDSNFYVFLIAMAMHALIIGVFGFFGIHAILWLQRGIVGLKRGEFVGHHSASGKYVRR
                     FKKRDIKMHILIIVTFLMLAATGLPLKFSYAPWAQTVVDLFGGVENTMFLHRLAAVGT
                     FGYFVWHLGILVQRFFLKKEKGLFWGPNSMTPQLKDGKDIVAMFKYFLYMGPRPAGDR
                     WTYWEKFDYLAVFWGMMIIGGSGLMLWFPNFFTQILPLPGWALNAAYVVHSEEALLAT
                     GFIFLFHFFHTHLRPESFPLDPVIFTGSQPLERFMDERPLEYQRMVESGTLEENLVPA
                     PTKEQERWIHIWGFLAVAIGLALAVGIFTALLIY"
BASE COUNT         1171 a         2262 c         2477 g         1162 t
ORIGIN      
        1 cggcatggag atgacgaagt agccgtcgat gaagcaggcg ttctggatcg gcaggatgtt
       61 ggggtggtcc agcttggcca gcatcctgac ctccttgcgg aagtcggcca ggaagtagtc
      121 atccatcatc cgccggtggg ggatcttcag cgcgacccgc tggccatgga tggtgtcgaa
      181 ggcctggtat acggcggcga gcggcccgtc ggacaggcgc cgctcgatac ggtatttacc
      241 cagtttctgc ctggccctca gcacttcatt gcggctctcg cggcgggttg ccgggccctc
      301 gccggccggc cccaaagtcc cggaatatag ggcaaaccgg gcgccggcgc gaggcattga
      361 attttctgcc gggggggcca tctaagggca tagacggcgc cggcggcacc ggaatgggcc
      421 gcccgacgaa caatggcatt gcaccaggta cttggagagc acggatcatg accggctacg
      481 agcgcaagga tttctctgac gaggcggatt tcgagttcac cggcgacgcg gaaccgatgg
      541 acctgctgcc ggacatcgaa cggcgcgaga cgcggcgcag cggacccgga acggcggccc
      601 gccggaccct cgaggcgctg gcggagagcc gccgcctgag ccgggagttg gccgagctgg
      661 accatttcga ggatatttga cgcgggtcga gcctgtgctc ggccggtcgc gggtgagaga
      721 atgagcttgg cggcgtgacg acgcgccgct ggaacggctc cgcggacgcg gggcaggagg
      781 cttcgacatg aatgctgtga cagctcgatc cgggttggcg gctgcggcaa tgctcgcgct
      841 ggcagcctgt accacggccg ggccagagat tcgcaccgat tacgatgcgg cgaccgattt
      901 ctcgcaattt agcaccttcg ccttcatgga ccgcgcggag cgcggcgaga cgcgcaccta
      961 cgacaccatc ggtgaccggc gcgtgatgtc gtccgtgcaa cgggaactcg aggcgcgcgg
     1021 ctaccgcagg gtggacagcg atcctgacct gctggtcaat ttcgcaatgg ccaccgagga
     1081 cgtgcaggac gtgcgcagcg tgccgtcgtc gatcgtgccg ccgccctggt acgggtggcg
     1141 tggcggctac tactacccgt ggccgaccta tgcgtacgag acctacgtgg acagctacca
     1201 gcgcggcacc ctctacatcg acctggtgga cgccgaacgg cgccagctgg tgtgggaggg
     1261 gcgcgcggtg ggacgtgtca ccagggccac ccgcgaggat ccggccgggg cgctggacgc
     1321 tgcggtggtg gagattttcg cgcagtatcc cttccgggcg ggtccgctgc gctagcggcc
     1381 ggtgtcgccc accgggctca gcggcgtcgc gtcggctccg ggtcggcgcg acgccttgcc
     1441 gggtcgcgcg ccaccacgtc gcaggccgcg ccctcgtggc gcgccgcgac gacccggtcc
     1501 gccaggatct cgaagtagaa ggtgcactcg ctgcgctccg gcacgacgag ggccgtccgg
     1561 tcacgctccg gatcgagccg cgcggcgggg tcggccagtg attccaggcg gcgatccagc
     1621 tcccggaagc gctcgaagac cagcaccgtg gcggtatcct cgccggtgag ggtgcgcagc
     1681 ggcgcccccc aggcggccag cagctcggag accggcgcac cctgccattc ctgcaggtcc
     1741 tgttgccacc agggttgagt gggggcgcag cccgccagga gcagcgccgc cgccggcagg
     1801 caggtgattc ggcagcggcg gttgcgggtc ggcatcacgg cccgatcata tcagcccgcg
     1861 tttcttcatg acggcgtgaa tgatccaggt gccgagaacc gccacgccga gcagcccgat
     1921 ggcgatgccc gcgttgttga gcgccgtcgg cgcgaacagc atcaccgcgc cgaggcccag
     1981 catcatcatg cccgacatca gcttcagcag tcgcccctcg cgctcgccca gcttgcgcga
     2041 gccgaacttc atcacgaaca gcagcacgat gaccagcagc gggatgacat agatgatgtt
     2101 gtacagcacc aggtacatgt aatagacgaa ggtgtcggct tcgcgcagcg tcaggatgcg
     2161 cgtgtagacc atcggcaggc cggcggtaca cagcagctcg tagctgttga cggccaccgc
     2221 cagcacggcg gtaccggcga gcatggcgtg gatgcttttc gcggcgacca ggttgcgcat
     2281 ccgctggtac atgccgggct tctttgattc cgggatggac aacgagggcc cgccggcgtc
     2341 gaaccagaag tagtccttga tgttgatcgc agccagcgtg acggccacca gcccggcgat
     2401 cagcgtcacc atgcgcagtt cgccgatcag caggaagacg ttcagccagg ccgccatgaa
     2461 gacgaaatag atgacgcccg agatcaccac gaaagtgccg ccgaccagca acatgcgcgc
     2521 ccggctgcga gcgtggacca tgaggctcaa caggaacagc agcacgaaga atgcacaggg
     2581 attgaaggca tccagtccgg cgatgatcac ggtgaggatg ggcaggcccc agtcggaagg
     2641 gttgatcagg ccgagcaccg gcacctcgac gcccatcacc ttgccgggct ccggcgcgcc
     2701 gccggactga gcgccgagca ccagctccgg gttggtgcgg cagtgttcca tgcgcgccag
     2761 cagcaccgtg cccgtggttt ccgggcgctc gaaaccggta tgcatcgagc cgcagaagat
     2821 gaacgcgggc acgctgcgcg cctgctcgcc ggcggcggcc gcgacgtcgc ggtacaggag
     2881 ggcgttgtcg cggttcttgg tgacctcgtg cgcatgcagc tcgatccagc cgagctcgtc
     2941 cagggaagcg acgaagggct tggcctcgat gcaatgcggg caggtgtcgg aatagaagta
     3001 gtagaccacg accctcgatt cgccgtctgg gccggtggtg aaccactcga ggtccgccgc
     3061 cagcgccgtc gatccgacca gcagggtcag cagcgcggcc aggcggcgaa agagcgtgga
     3121 atgcagcatc gagcctcccc gaagtctgga gcggcgcact ctactgggcg tgccgggcac
     3181 aggcaatcgt ggcaatcccg ggtttccggg tcgccgtccg cttgactggc gccacgcgaa
     3241 gcccgacctt gtaggcgcga tgatgcgcga gatcatagac cagtccgtcc gccatgcacg
     3301 gcggctcatc gtggccgtgg tcggcgtcac ggtcctgttg ttcggcctgg cgctggtgat
     3361 cctgcccggg ccggccttcg tggtgatccc gctcggcctg gccatcctgg ccgcggagtt
     3421 cgcctgggcg cgcagcctgc tgcgccgcgt ccggcgcggc atcagcaacg ggctgcgcaa
     3481 cggccggctg aaccgctagg gcccggttcc ctacggattg agcagccgca ggatctccgg
     3541 gtcctcctcc cgctgggcca gcaccgtgtg gcacaggtcg cagctgcgcg agatgcgctc
     3601 gccctcggcc gtctggtgct gccggttgtg gcagcggaag cagccgggcg catcctggcg
     3661 atcgcggacc ggcgggtggc cgctgtggtt cgggtaggtg tcccagcgca ctttcatgtg
     3721 cgggaagacg ttccagctgt agacgtcgcc gagggcgtcc gcggccgccg tcacggcgac
     3781 ggcgttctgc gccagcacct ccggatagtt ctcgcggtag aaggcgttga gcgactccgt
     3841 gatgccgact cgtgctgcgt cgtgcgtctc gtaattcgcc tcctcgatga tcctgacgcc
     3901 ctcgcgcttg acgtagggca ggccggtgtc gatcttgccg gcggccagca gcaggtcgat
     3961 ctcctcggcc ggcgcgcggt agatgtgggt cggccggttg tggcagtcga cgcagtccat
     4021 gacgcgccac tcggcgtccg ccggagcctc gccggccagg aacaccttca cgctgccgtc
     4081 ggccttggtc atctcgacgt cgtagatgtt catccggtcg gggtccgaga ggtagcggat
     4141 ctggatgttc gggtccacgt gccagtgaat gccggaggat tcgcgcccgt gcaggccgcc
     4201 caccttcagc agcacggcgc tgcgcagctc ggtattgagc tcgtcctcgt tgtagacggt
     4261 gtcgatcttg agcttgtcgc cgacgaactt ggtcggccag tggcactgtt cgcaggtgtc
     4321 gcgcgccgga cgcaggttgt gcagcggggt ctcgatcggt cgcggataga tgttcagcgc
     4381 caccgcaacc agctgccagg cgccgtcgag cttggatttc acgaaccagt cggcgccggg
     4441 gccgatgtgg cagtcgacgc aaccgacccg ggagtgcggc gagcgctggt gggccgtgtg
     4501 ctcgggctgc atgaccttgt ggcaggccat gccgcagaac tccgtggagt ccatgatctc
     4561 cacgcctttg taggtcgcgc cggccagcac gatgatgttc accatggtca gcaccatgaa
     4621 cacgatgacc gacttgcggg tggtctcctg gttcaggtcg atgatcggga acaacggcgg
     4681 tgcctcgccc gccgccgcgg cccgcgcggc ccgcttgcgc tggcgcatga tcccgaaggg
     4741 gatcagcagc aggccgagga cgaagaacat cggcagcacc aggaaggtca ggatgccgag
     4801 gtagggccca ccctcgaagc cgaccatctg cacgatgaac aacgcgacga tcagggtcag
     4861 cgaggccgcg gccagcgcgg tgccgacgag gctgatcggg ttctgggtca gcgaagcgaa
     4921 gaagtgtctg gacatctgtt gttttccttc atcgagacga acagggcccc atcgatcgct
     4981 cgacgaggcc ctgcgccagt ccttggttga tgatccgggc ggccttcctg gccgcccgga
     5041 ctgtggcccg catcaatata tcagcagcgc cgtgaagatg cccacggcga gggccaggcc
     5101 gatcgccacc gccaggaagc cccagatgtg gatccagcgc tcctgctcct tggtcggcgc
     5161 cggcacgagg ttctcctcca gcgtgccgct ctcgaccatc cgctggtact ccagcgggcg
     5221 ttcgtccatg aaacgctcca gcggctggct gccggtgaag atcaccgggt cgagcgggaa
     5281 gctttccggc cgcaggtgcg tatggaagaa gtggaacagg aagatgaagc cggtggcgag
     5341 cagcgcttcc tcgctgtgca ccacgtaggc cgcgttcagc gcccagcccg gcagcggcag
     5401 gatctgggtg aagaagttcg ggaaccacag catcaggccc gaaccaccga tgatcatcat
     5461 gccccagaac accgccaggt agtcgaactt ctcccagtag gtccagcggt cgccggccgg
     5521 ccgcgggccc atgtacagga agtacttgaa catcgcgacg atgtccttgc cgtccttgag
     5581 ctgcggcgtc atggagttcg gcccccagaa caggcccttc tccttcttga ggaagaagcg
     5641 ctgcaccagg atgccgaggt gccagacgaa gtagccgaag gtgccgacgg ccgccaggcg
     5701 gtgcaggaac atcgtgttct cgacgccgcc gaacaggtcg accaccgtct gcgcccaggg
     5761 cgcatagctg aacttcagcg gcaggccggt cgccgccagc atcaggaagg tcacgatgat
     5821 caggatgtgc atcttgatgt cgcgcttctt gaagcggcgc acgtacttgc cgctggcgct
     5881 gtggtggccg acgaactcgc cgcgcttcag gccgacgatg ccgcgctgca gccacaggat
     5941 ggcgtggata ccgaagaagc cgaacacgcc gatgatcagc gcatgcatcg ccatcgcgat
     6001 caggaacacg tagaaattgc tgtccggatc gctcgagtcg atgtgcggat tgtacgaggc
     6061 aaagcccgcg gtcacggcgc cgtggcagtc gccgcaggtc gcctgcaggt tgtcggtatg
     6121 aatggtcgag cgcgggtcgt ccttgcccag cgtggcgtgc ggatggtggc agtccgcgca
     6181 ggtggcggac accgccaggc cgagattggt ggccttgccg tggaagtcgc cgcggtaaag
     6241 ctggtaatag tcgccgtggc agtcgccgca ctggtcgacg atgccgagac gctcggcccg
     6301 gtcggtgacc gggtcacgga tctcgtgcga ggagtggcag tcgatgcagg tcgcggtccc
     6361 ggtgttgccg gccgtccagg cgacgccatg ggcgctgacg ttcagccact cctggtacac
     6421 gtactggtgg cagtcgccgc acatctgcgg cgagttctgg aacgaggtcg gcgagcgctc
     6481 gttctcgacc tcgaccaccc ggtggctgcc atggcaggtg ctgcaggtcg gcgagctcac
     6541 cagcccggag cgcagcaggc cgcgaccgtg gacgctgtcg acgaaggaat cgatcatgtg
     6601 cttctggccg ggcagctgcg cgtggcaatc accgcaggtc tgcacctggc cgacgggaga
     6661 catcggggac agcaggtcgt cgttcttgcg catgacgtgc gcatcgccat ggcattcgac
     6721 gcaggcgacc ttgtcgccgt gcacgctgcg gcccatggcg cgggtggcca tggagtgaca
     6781 ttgcgcgcag atcgcgtcgc ccgtcaggtc ggccaggtcc tctttcttgt ggcgcggccg
     6841 ggtgacgttg gtgtggcagt ccttgcaggc cacctccgcg tgcgcggtcg aatcgaaatg
     6901 cacgtcgtcg tggcacgcca tgcaattgat ctcgcgctcc tgggcgttga ccgcggtcgc
     6961 ggccagcgac agcgccgcca cggccagcag gccgcgcagg agggcaggga aggaagtgtg
     7021 tctggccatg gctgtgatcc ctttgatgcg gttcccgacc gcgggccccg cg
//
LOCUS       DAOMPG010000113         7068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4558_length_7068_cov_3.881791, whole genome shotgun sequence.
ACCESSION   DAOMPG010000113 DAOMPG010000000
VERSION     DAOMPG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_7068_cov_3.881791"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="sucA"
                     /locus_tag="PLS34_07425"
     CDS             complement(<1..692)
                     /gene="sucA"
                     /locus_tag="PLS34_07425"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRP87335.1"
                     /translation="MSKTLQDRYATSPLFGGNAPYIEGLYEQFLEDPASVEPALREWF
                     DGIANGGGDMPRGPVEREFAARALRPRAAAATGAVPDAILDKQAAVLRLIEAYRLRGH
                     RLAKLDPLGLVGPQPLPELEPAFHGLGPSDDDTVFATAGFGGADRLSFREIRQRLGRV
                     YRGTIAAELGHITDTEERLWLQRRFEAAAAGGGLAPDDRRHLLERLTGAEGIERYLHT
                     RYVGQKRFSLEGG"
     gene            907..2289
                     /locus_tag="PLS34_07430"
     CDS             907..2289
                     /locus_tag="PLS34_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87336.1"
                     /translation="MRLSVLLLSFLISGQAGALGSSLYPDLMEFDEAMALYRAGELEP
                     ARRAFSHLAELGDPAAQFNLGVMLVKGEGGEPDAITGTAWLQWAADSGLAGAAEVLAI
                     MASRLDEDAQKQVAQALAGLQAGGSPSDRRTGPSDTDDGYWACFERNRHQPPPIYPMR
                     ALDQDRVGFAMLHFLVGPEGNFDAIHAIPDGHHYGPFGPAAQEGARRWHAHGCRTQRY
                     RHMTQSVVFEIVDGFGDPSISPARKQWAGKVLAEARAGSPEQSYIVGMLASLYETYFA
                     VDAEERLSLLHRAAVAGFPDARYEYWRAAGARGERWLLLAARQGYPPALFALRKRQHL
                     PREDRRRALHRAAEAGFLPAVYLAVRHLAAHPDASQRDGARALQLSSVVHSRILRGDP
                     SMAEVHAMALAENGQFKDAVSWQKRAVSTGRRLGRDTTGAAARLARYEAGEPWRDPHL
                     SSDPGGEDDG"
     gene            2338..2991
                     /locus_tag="PLS34_07435"
     CDS             2338..2991
                     /locus_tag="PLS34_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87337.1"
                     /translation="MAITSLKRLLYRLSVALALALVAAAGLADDLLPLEHSLRYVGHQ
                     GRTPVVVEITLRERLDGELEYVEWVTPADWGAWFREPAATRCRLRYRDGILATMDVDH
                     GLGAKRPPADVSAGALDALSVRLRARADIARGTKHAEYTVWNGGEALETWILEVAGPD
                     SVTTPDSRYEALRFRLGSDREWFEGWSAPLLVFHFVRIEHWRDGKKVGELSLDDKQL"
     gene            complement(3023..4474)
                     /locus_tag="PLS34_07440"
     CDS             complement(3023..4474)
                     /locus_tag="PLS34_07440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP87338.1"
                     /translation="MRLTFHGAAGGVTGSCYLIECGGRSLLLECGLLQGGPQEDLGNR
                     APFPVDPAGLDAVVLSHAHIDHSGRLPLLVKQGFRGRIHTQRATAELCEIMLRDAGYL
                     QEKDAEIENRKRERKGLSRIAPLYTRLDAERAMRRFQAHDYDEIIEVMPGVRARFRDA
                     GHILGSAVVELWLNEDGQTRKLVFSGDLGQPNTPILRDPVKVPDADAVLMESTYGDRL
                     HRSREETFAEMKEIFATARAGGGNILIPAFAVGRTQELLYLFAEHFEEWGLADWRICL
                     DSPMAIAATELYARHVDLHDEEALRLWRRGDDALAERLEFVRTPQQSRALNRVRSGLI
                     IVAASGMCEGGRIRHHLKNNVWRPDCHVVIVGFQARGTVGRQLVDGAASIRLWGESIR
                     VAATVHTVGGFSAHADQQGLLDWYRGFADAPPVWLVHGEDEARAGLAARLEAEADAGV
                     HLPRAGDGIDFLQLPARRKPGSDPHFCCAEMGV"
     gene            complement(4484..5122)
                     /locus_tag="PLS34_07445"
     CDS             complement(4484..5122)
                     /locus_tag="PLS34_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87339.1"
                     /translation="MWRSLLQFGLALTLPGLVPLAADAHEPPPIVPFCTMCHGDTGPS
                     PYPGVPTIHGLPGGVIEAALYNYRERHRPCRVTECSKLGTCPDISMCDIAGGMTDDEI
                     DLLAYWYSRQPFPAHQDPYNPELAARGREIHERYCEVCHTKLGSDPIDDASMLRGQRK
                     VYLRNALEDFKAGRRSVGLAAMDDRFEEFDERDIAALAEFFSGPAAYPLPDE"
     gene            complement(5145..5765)
                     /locus_tag="PLS34_07450"
     CDS             complement(5145..5765)
                     /locus_tag="PLS34_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP87340.1"
                     /translation="MLKLLLACGCLLLWMSGPVAAESGAPADVAVCAACHGDEAPSPF
                     PSVPTIHGLPQAVLDNALFDFRATIRPCRKPDCGAAADCPDIDFCSIAAALSDEQISA
                     LASWYASRRFVAAGEPFDAALAARGAQLHQDKCDSCHTEGGTSSTEQATILRGQRKAY
                     LRLALEDFRQERRVAVAEMDAMIRALSDDELTALVEFYASPRRPKQ"
     gene            5989..6510
                     /locus_tag="PLS34_07455"
     CDS             5989..6510
                     /locus_tag="PLS34_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019933475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRP87341.1"
                     /translation="MKTHVRLLAAAAMLALAVPVLADHHAAKGKAYGDKARADIVDTA
                     VAAGSFATLVAAVQAAGLVDTLKGDGPFTVFAPTDEAFAALPAGTVESLLQPENREQL
                     IAVLTYHVVPGKVTAADVVKLSDATTVNGAAVSISSSYGKVKVDGANVVQADVMASNG
                     VIHVIDQVILPGT"
     gene            complement(6572..6647)
                     /locus_tag="PLS34_07460"
     tRNA            complement(6572..6647)
                     /locus_tag="PLS34_07460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6612..6614),aa:Arg,seq:cct)
     assembly_gap    6752..6761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6762..>7068)
                     /locus_tag="PLS34_07465"
     CDS             complement(<6762..>7068)
                     /locus_tag="PLS34_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP87342.1"
                     /translation="LSVAALAQHMDMSARHFSRSFSEEAGVTPAKFVERARFEAAQRM
                     LVEDDLDLETVAARCGFGSVETLRRVFHRRTGMATAAYRLRLQREKHGDQAGPSCPGL
                     "
BASE COUNT         1114 a         2431 c         2399 g         1114 t
ORIGIN      
        1 ccgccctcga gcgagaaacg cttctgcccg acgtagcggg tgtgcaggta gcgctcgatg
       61 ccctccgcgc cggtgagccg ctcgagaagg tggcgacggt cgtcgggcgc caggccgccg
      121 ccggccgcag ccgcctcgaa gcggcgttgc agccagaggc gttcctcggt gtccgtgatg
      181 tggccgagtt cggcggcgat ggtgccgcgg tagacccggc ctagccgctg cctgatttcc
      241 cggaaactca gccggtccgc gccgccgaaa ccagccgtgg cgaacacggt gtcgtcgtcc
      301 gaggggccga gcccgtggaa ggccggctcg agctccggca ggggctgcgg cccgaccagg
      361 ccgagcggat cgagcttcgc cagccggtgg ccgcgcagcc ggtaggcctc gatcagccgc
      421 agcaccgcgg cctgcttgtc gaggatcgcg tcgggcacgg cgccggtcgc cgccgcggcg
      481 cgcgggcgca gcgcccgggc ggcgaactcg cgctccaccg ggccgcgcgg catatcgccg
      541 ccgccgttcg caatgccgtc gaaccattcc ctgagggccg gctcgaccga cgccggatcc
      601 tcgagaaact gctcgtaaag cccctcgatg tagggtgcat tgccgccgaa cagcgggctg
      661 gtcgcgtacc gatcctgcag cgtcttgctc atcggagtgg gaagtcgggc cttggattgt
      721 gggggcagcc acccattcta agcgaagcgc ccggcggttg gaatgtaagc ccatggccgg
      781 ccgggcaatt tgggacggcg ttcgcgcttc ggcggggtcc gggccggcag cggatttctg
      841 cttgtcggcc tcttgcctga gccgtatgct cggctggctc tcgccatatg tggggaccca
      901 gggccaatgc gcttgtccgt cttgctgttg tcattcctga tctccggcca ggcgggcgcg
      961 ctggggtcca gcctgtaccc ggacctgatg gagttcgacg aggccatggc cctctaccgg
     1021 gccggcgagc tcgagccggc ccgccgcgcc ttttcgcacc tcgccgaact cggcgacccg
     1081 gcggcgcagt tcaacctcgg cgtcatgctg gtcaagggcg aaggcggcga accggatgcc
     1141 atcaccggca ctgcatggct ccaatgggcg gcggattccg ggcttgctgg cgccgcagag
     1201 gtgctggcga tcatggcgag ccggctcgac gaagatgcgc agaagcaagt cgcgcaggcg
     1261 ctggccggcc tgcaggccgg aggttcgccc tccgaccggc gaaccgggcc atccgacaca
     1321 gatgacgggt actgggcctg ctttgagagg aaccgccacc agcccccgcc gatctatccc
     1381 atgcgggctc ttgaccagga ccgcgtcggc ttcgccatgc tccatttcct ggtcggtccg
     1441 gaaggcaact tcgatgccat ccacgccatt ccggacggcc accactacgg accgttcggc
     1501 ccggccgcgc aggagggtgc gcggcgatgg catgcccatg gatgccgcac gcaacgttat
     1561 cgccacatga cgcagtcagt cgtcttcgaa atagtggatg ggttcggcga cccgtccatc
     1621 agcccggcgc gcaagcaatg ggccgggaag gttctggccg aggcgcgggc aggcagcccc
     1681 gaacagtctt acattgtcgg catgttggcc agtctctacg agacgtattt cgcggtcgat
     1741 gcggaagaac gcctcagcct gctccaccgg gccgcggtgg ccggctttcc ggacgcccgt
     1801 tatgagtact ggcgggctgc tggcgcccga ggggaacggt ggctgctgct ggcggcacgc
     1861 cagggttacc cgccggcgct cttcgcgttg cgcaagcggc agcacctgcc tcgcgaggat
     1921 cgtcggcggg cattgcaccg cgccgccgag gccggcttcc tgccggccgt gtacctcgcc
     1981 gtgcgtcatc tggcagcgca tcctgacgcc tcacaacgcg atggtgcacg ggcgctgcag
     2041 ctgagctccg tggtgcacag ccggatcctg cgcggcgacc cctcgatggc cgaggttcat
     2101 gccatggcgc tggcggaaaa cggccagttc aaggacgccg tgagctggca gaaacgcgca
     2161 gtcagcaccg gccggcgcct ggggcgcgac accacgggcg ccgcggcgag gctggctcgc
     2221 tacgaggccg gcgaaccctg gcgcgacccc cacttgtcat ccgacccagg cggggaggat
     2281 gatggctgac aggccgttcc ggcggccgga tcttcgcttt gcggggtctg actctcgatg
     2341 gcgatcactt ccctcaagcg gctgctgtat cgcttatcgg tcgccctggc gctggcgctg
     2401 gtcgccgcgg ccgggctcgc cgacgacctg ctgccgctgg agcacagcct ccgctatgtc
     2461 ggccaccagg gacgcacgcc ggtcgtcgta gagattaccc tgcgcgaacg cctcgacggc
     2521 gaactcgaat acgtggaatg ggtgaccccc gccgactggg gcgcctggtt cagggagccc
     2581 gctgcgacgc gctgccggct gcgttaccgg gacgggatcc tggctaccat ggacgtggac
     2641 cacggccttg gcgccaagcg tcccccggcg gacgtctctg cgggtgcgct cgacgccttg
     2701 tctgtcaggc tccgcgcccg cgccgacatc gcacgcggta ctaaacacgc cgagtacacc
     2761 gtctggaacg gcggcgaggc gctggagacg tggatcctcg aggtcgccgg tccggacagc
     2821 gtgacgacgc ccgacagtcg ctacgaggcg ctccggttca ggctcgggtc ggaccgcgaa
     2881 tggttcgagg gctggtccgc gccgctgctg gtgttccact tcgtgcgcat cgagcactgg
     2941 cgcgacggca agaaggtcgg tgagctgagc ctcgacgaca agcagctctg acgtcgcgtc
     3001 cgaaaaccgg ggaaaaccgg gatcagaccc ccatttccgc gcagcagaaa tgggggtctg
     3061 accccggttt tcggcgcgcg ggcagctgga ggaagtcgat gccgtcaccg gcccgtggca
     3121 aatgcacgcc ggcgtcggcc tcggcttcga gccgggcggc cagcccggcg cgcgcctcat
     3181 cctcgccgtg gaccagccac acgggcggcg catccgcgaa tccccggtac cagtcgagca
     3241 gcccctgctg gtccgcatga gcagaaaaac cgccgacggt atgcaccgta gcagcgacgc
     3301 ggatactctc accccacaac cggatgctgg cggcgccgtc caccagctgc cggccgaccg
     3361 tgccgcgcgc ctggaagccg acgatgacca cgtggcagtc tgggcgccag acgttgttct
     3421 tgaggtgatg acggatgcgg ccgccctcgc acatgccgct ggcggccacg atgatcagcc
     3481 cggaacgcac gcggttcagg gcgcgcgact gctggggtgt gcgcacgaac tcgagccgct
     3541 ccgccagcgc atcgtcgccg cgccgccaca ggcgcagcgc ttcctcgtcg tgcagatcca
     3601 catgcctggc atacaactcg gtggccgcga tggccatcgg gctgtcgagg cagatacgcc
     3661 agtcggccag gccccattcc tcgaagtgtt cggcaaacag gtagagcagc tcctgggtgc
     3721 gtccgaccgc gaacgccggg atgagaatat tgccgccgcc ggcccgggcg gtcgcaaaga
     3781 tttccttcat ctccgcgaag gtctcttccc gcgagcggtg caaccggtcg ccgtaggtgg
     3841 actccatcag cacggcgtcc gcgtccggga ctttcaccgg gtcgcgcagg atcggcgtgt
     3901 tgggctggcc gagatccccc gagaacacca gcttgcgggt ctgtccgtcc tcgttcagcc
     3961 agagctcgac gacggccgag ccgagaatgt gcccagcgtc cctgaagcgc gcccgtaccc
     4021 cgggcatgac ttcgatgatc tcgtcgtagt cgtgcgcctg gaagcgtcgc atcgcccgct
     4081 cggcgtccag ccgggtgtac agcggggcga tccgggacag gcccttgcgc tcccgcttgc
     4141 ggttctcgat ctcggcgtct ttttcctgca ggtagccggc gtcccgaagc atgatttcgc
     4201 agagctccgc ggtcgcccgc tgggtgtgaa tgcggccccg aaaaccctgc ttgaccagca
     4261 gcggcaaccg cccggaatga tcgatgtggg catggctgag tacgacggca tccaggccgg
     4321 ccgggtccac cggaaacggc gcacggttgc cgagatcttc ctgcggcccg ccctgcagca
     4381 gcccgcactc gagcagcagg ctgcggccgc cgcactcaat gaggtaacag ctgccggtga
     4441 cgcccccggc cgcgccgtga aaagtcagcc gcatcgaagc gacctactcg tccggcagcg
     4501 ggtaagcggc cggcccggag aaaaactccg cgagggcggc gatatcccgt tcgtcgaact
     4561 cctcgaagcg atcatccatc gccgccaggc ccaccgagcg cctgccggcc ttgaagtcct
     4621 cgagcgcatt gcgcaggtat accttgcgct ggccgcgcaa catgctcgcg tcgtcgatcg
     4681 ggtccgagcc cagcttggtg tgacagacct cgcagtagcg ctcgtggatc tcgcgtcccc
     4741 gggcggccag ctccgggttg taggggtcct ggtgcgcagg gaacggctgc cgtgaatacc
     4801 agtacgccag caggtcgatc tcgtcgtccg tcatgccgcc ggcgatgtcg cacatactga
     4861 tgtccgggca ggtgcccagc ttggagcact cggtcacccg acagggacga tgacgctcgc
     4921 gatagttgta gagcgccgcc tcgatgacgc cgcccggcag gccgtgaatg gtcggcacgc
     4981 ccggatacgg gctcggcccg gtgtcgccgt ggcacatcgt acagaacggg acgatcggcg
     5041 gaggttcatg ggcatccgcg gcgagcggca cgaggcccgg gagagtgagc gccagaccga
     5101 actggagcag ggatcgccac atggtcattt cctcctggaa gccatcattg cttcggccgg
     5161 cgcggactgg cgtagaactc caccagggcc gtcaactcat cgtccgagag cgcacgaatc
     5221 atggcgtcca tctcagccac ggccacccgg cgctcctgcc ggaaatcttc cagcgcaagc
     5281 cgcaggtagg ccttgcgctg gccgcgcagg atggtggcct gctccgtcga ggaagtgccg
     5341 ccttccgtat gacagctgtc gcacttgtcc tgatgtagct gcgcaccgcg ggctgccagc
     5401 gcggcgtcga agggctcgcc cgccgcgacg aagcggcggc tcgcgtacca gctcgcgagt
     5461 gcgctgatct gctcgtcact cagtgcggcg gcgatgctgc agaaatcgat atcggggcaa
     5521 tccgccgcag cgccgcaatc gggcttgcgg cagggccgga tggtggcgcg aaagtcgaac
     5581 agggcgttgt ccagcaccgc ctgtgggagg ccgtggatgg tcgggaccga ggggaagggg
     5641 ctgggtgctt catccccatg gcaggctgcg caaaccgcaa cgtccgcggg cgccccgctc
     5701 tccgccgcga ccggaccgct catccacagg agcagacaac cgcacgcaag aagcaacttc
     5761 agcatcatgc atctccctgt ctcgcagtga aaatatcaca ggacaccgac ccgtgctccc
     5821 gtcgcgccaa caaggctgcc gccgggggtc cgcgccgtcc gccgggcggt accggccggt
     5881 cgtcccggca cgtcgcgaat ccacgtcgcg aggcgcgccg aaagcatcgc cgtagacaca
     5941 gcgtgccacg cccggcgcgc agcaaatcac tgccaaggag aattttgcat gaagactcat
     6001 gttcgcttgc ttgcggccgc cgccatgctt gcgctcgccg ttcccgtgct cgccgaccac
     6061 catgccgcca agggcaaggc ctacggcgac aaggcccggg ccgatattgt cgacaccgcg
     6121 gtggcggcgg gctcctttgc cacgctggtc gcggcggtgc aggccgccgg gctggttgac
     6181 acgctcaagg gtgatggccc gttcacggtg ttcgcgccga ccgacgaggc ctttgccgcg
     6241 ctgccggcgg gcacggtcga gtcgctgctg cagccggaaa accgtgagca gctgatcgcc
     6301 gtgctgacct atcatgtggt gcccggcaag gtgaccgccg ccgacgtcgt caagctgtcc
     6361 gacgctacga ccgtgaacgg cgccgccgta tcgatctcgt cgagctacgg caaggtcaag
     6421 gtcgacggag cgaacgtggt gcaggccgac gtgatggcca gcaacggcgt gatccacgtc
     6481 atcgaccagg tgatcctgcc cggcacctga aacgcccact cccacggacg tgccggcccc
     6541 tgcggccggc acgtcttttt tctttgctgt ctggcgcccc gggcaggaat cgaacctgcg
     6601 acctaccgct taggaggcgg tcgctctatc cactgagcta ccggggccgc ggggcgcggt
     6661 attgtgccat ccgggcggcg ctgctcacaa accggggtcc gagagtcctg ccccgtccac
     6721 caacgcctgt ctttgcaagc gcaggcggta gnnnnnnnnn ncaaaccggg gcacgaaggc
     6781 cccgcctgat cgccatgctt ctctctttgc aggcgtaacc ggtaggcggc agtcgccatt
     6841 ccggtacggc gatgaaacac gcgacgcagc gtctctacac tgccgaaacc gcagcgagcg
     6901 gcgacagttt ccaggtcgag gtcgtcctcg acaagcatcc gctgcgctgc ctcgaaacgg
     6961 gcccgctcca cgaacttggc gggcgtcaca ccggcttcct cgctgaagct gcggctgaag
     7021 tgccgcgcgc tcatgtccat gtgctgcgcc agcgcggcca ccgacaag
//
LOCUS       DAOMPG010000114         7058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4572_length_7058_cov_3.929602, whole genome shotgun sequence.
ACCESSION   DAOMPG010000114 DAOMPG010000000
VERSION     DAOMPG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4572_length_7058_cov_3.929602"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLS34_07470"
     CDS             complement(<1..827)
                     /locus_tag="PLS34_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP87343.1"
                     /translation="MSISRRVRAIASKEVRQLRRDRLTLGMVIGIPLMQIVLFGYAIN
                     LDVRHLRAAVADEADTQYSRQLVMSLQASQVIELVAAARNAAELEDLMRAGDIVVGVY
                     IPPDFERRLQDPMRPAAQLLVDGSDPAILGVAGQLVAAPPPGRVPATATAAPVFELRN
                     YYNPERRSAVQIVPALIGVILTMTMVLFTAVAIVRERERGNLEFLITTPVGTLELMLG
                     KLLPYVLIGLLQVSLVLIVGHLLFGVPFRGQLGDLYAAALVFIAASLALGLLISTLAR
                     "
     gene            complement(824..1756)
                     /locus_tag="PLS34_07475"
     CDS             complement(824..1756)
                     /locus_tag="PLS34_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP87344.1"
                     /translation="MVATGLTRRFGALVAVDALDLDVPRAAIYGFLGPNGSGKSTTIR
                     LLCGLLLPTAGRVTVLGYEVPGQAEAVRRRIGYMTQRFSLYEDLSVRENLDFVAEVHG
                     LPAREKRARLAEAMTEYRLAGLAKQRAGTMSGGQRQRLALAAATLHRPELLLLDEPTS
                     AVDPQSRRDFWDSLFELAGRGTTILVSTHYMDEAERCHRLAILDRGRLAAEGAPRELM
                     QGLAGDVVEVKTAGTLQARRVLESLPAVISITQLGTRLRALVERHAGIDPAASLGNAL
                     AAVGLPAEVERVPASLEDVFVAATRRAAAAECAA"
     gene            complement(1771..2742)
                     /locus_tag="PLS34_07480"
     CDS             complement(1771..2742)
                     /locus_tag="PLS34_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRP87345.1"
                     /translation="MNRCLSLCLLLTMLAACGGDGTGLPAVGTLERDRIEIVAEAHET
                     LLEMHAREGAAVAAGEVLARQDAARRQTAVAHAEAARTRAARRVDELLRGPRRESIAE
                     AQAGLDGARRRLTLERREYERLQALVERGLVSAAERDGAQLRRDAAEAERRQWEARVE
                     ALLEGTTVEELDQAVAALAEAEAVLADARLAVERLVLRAPRDGVVDAIPYKRGDRPQP
                     GAVVIVMLADQAPHARVYVPQAVRARVHAGTAAEVRVAGYEQVFAATVRTISAEAAFT
                     PYFALTERDRGRLVYLAELVLTEPAARDLPAGLPVQVDFPALGLAAQ"
     gene            complement(2739..3353)
                     /locus_tag="PLS34_07485"
     CDS             complement(2739..3353)
                     /locus_tag="PLS34_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP87346.1"
                     /translation="MGRPSEQSRKEVRARLGTAARELFARHGFEAVSLKQVAEAAGVT
                     PAMVHYYFGGKHGLWLAVLEDYLEEALGSMLAVRDEIEGPGGLDVYLARHTELLGRQP
                     WVAPLLFRELILGDGPGADFVRRFPSRLRDLLRSAVEHAKRRGEIEPGLDPDLIVLSL
                     LSAAVFPFLVRPLAEQLFAAPVDIAFAARWSAHAARMFHRGARP"
     gene            3505..4497
                     /locus_tag="PLS34_07490"
     CDS             3505..4497
                     /locus_tag="PLS34_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRP87347.1"
                     /translation="MSKIFAALLALTVALPSALAQPRAEPVLLIHGGAGTLRAADLSD
                     ADRARYEAALHEALDAGWRVLAGGGSALDAVTAAILPMEDSPLFNAGRGAVFTADGRN
                     ELDASIMDGATRAAGAVGGVRSVRHPVLLARRVMEASPHVFLAGPGALEFAIDQGLEL
                     KPAEWFWTERRWQQLQRATLRNETGAAPGDAGDDRFGTVGAVALDAAGQLAAATSTGG
                     TTAKRYGRLGDVPVIGAGTWADAGCAVSATGDGEYFIRAAAARSICARMAWTGAAPGD
                     AAAAVLQEISALGGLGGVIVVDGAGRFSMLFSTEGMYRGHADARGRQVAIFARD"
     gene            complement(4559..5512)
                     /locus_tag="PLS34_07495"
     CDS             complement(4559..5512)
                     /locus_tag="PLS34_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRP87348.1"
                     /translation="MIVHVGFLVLGSVLLYAGGEGLVRGAGTMALRLGMSPFIIGITI
                     VAFATSAPELAVSLQAALAGVDDVAIGNVVGSNIANIGLILGICAVLRPVKIEARILS
                     LDLPWLIFVSLLLVAFLNDGHISRPAGLVLLAGLGVFLYWNIQVARREQEVERVRQEF
                     EGVAPRVQESAFMDWVFIVGGLVALVAGGAAFVRGGVGLATALGVSPALIALTVIAVG
                     TSLPELATSIVASLKGNDDIAVGNVIGSNFFNILAILGITAVLRPLDRGDITMVDLVV
                     MLMLALMLVPLIMVRQRIGRPEGLALLAAYFAYIAWLARQA"
     gene            complement(5509..7047)
                     /gene="gshA"
                     /locus_tag="PLS34_07500"
     CDS             complement(5509..7047)
                     /gene="gshA"
                     /locus_tag="PLS34_07500"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRP87349.1"
                     /translation="MDRGCLSGGGRGVEKESLRVAPNGRIAATPHPRALGSALAHPWI
                     TTDYSEALLEFVTPPQQSCWETLQFLCDIHQFVYPRLPDDEMLWATSMPCAVGGDASV
                     PVAEYGSSNIGRMKHIYRLGLGYRYGRVMQTISGVHFNYSLPEPFWQVFREVEGWQGT
                     EREFRDRMYFGLLRNFRRFGWIVLYLFGASPAICKSFMGGRDPGLPEFDPATIYGPHA
                     TSLRMSDLGYRNTSQASIHLSMNTLDEYVAGLEQAIRSPWPPYEKIGVVSAGEWRQLS
                     TSALQIENEYYGFIRPKQPIRPGERPTLALRRAGVAYMEVRALDVSPYDPAGVSQNEF
                     RFLEAFLIFCMLHDSPPITAGELELLDDNHARVAVTGRCPGLALAISGVKKPLAEWGR
                     EICRQLVPICRLLDGNDPLRSYSAALELQAAKIEDVSLTPSARILAEMRQEGESFFQF
                     ALRMSRQHQEYFAALPPMSPERLQEFEQEAARSLEAQQAMEAGPAEPFEEFLASYFAM
                     AEAG"
BASE COUNT         1102 a         2541 c         2440 g          975 t
ORIGIN      
        1 cgcgccaggg tcgagatcag caggcccagc gccaggctgg ccgcgatgaa caccagcgcg
       61 gccgcgtaca ggtcgcccag ctggccgcga aacggcacgc cgaacagcag gtggccgacg
      121 atcagcacca gcgaaacctg cagcaggccg atcagcacgt acggcaggag cttgccgagc
      181 atcaattcga gcgtgcccac cggcgtggtg atcaggaact cgagattgcc gcgctcgcgc
      241 tcgcgcacga ttgccacggc ggtgaacagc accatggtca tggtcaggat gacgccgatc
      301 agggccggca cgatctgcac ggccgaacgg cgctcggggt tgtagtaatt gcgcagctcg
      361 aacaccggcg cggcggtggc ggtcgccggg accctgcccg gcggcggcgc ggcgacgagt
      421 tgccccgcca cgcccaggat cgccggatca ctgccgtcca ccagcagctg ggccgcgggc
      481 cgcatcgggt cctgcagtcg ccgctcgaaa tccggcggaa tgtagacccc caccacgatg
      541 tcgccggccc gcatcaggtc ctcgagttca gccgcgttgc gcgccgccgc cacgagttcg
      601 atcacctggc tggcctgcag gctcatcacc agctggcggg aatattgcgt gtccgcctcg
      661 tcggccaccg cagcgcgcag gtggcgcacg tccaggttga tggcatagcc gaacagcacg
      721 atctgcatca gcggaatgcc gatcaccatg ccgagcgtca ggcgatcgcg gcgcagctgg
      781 cggacttcct tgctggcgat ggcgcggacg cggcgcgaaa tgctcacgcg gcgcactccg
      841 cagccgccgc gcggcgggtc gccgcgacga acacgtcctc gagactggcg ggcacgcgct
      901 cgacctccgc cggcaggcca accgccgcca gggcgttgcc gaggctggcg gccggatcga
      961 tacctgcgtg ccgttcgacg agcgcccgca ggcgcgtgcc gagctgggtg atgctgatca
     1021 ccgcgggcaa ggactccagg acccgccgcg cctgcagcgt gccggcggtc ttgacctcga
     1081 ccacgtcgcc ggccagcccc tgcatcagct cgcgtggcgc accctcggca gcaagccggc
     1141 cgcggtcgag gatcgcgagc cggtgacagc gctcggcctc gtccatgtag tgggtcgaga
     1201 cgaggatggt cgtaccgcgg cccgccagtt cgaacaggct gtcccagaaa tcgcggcggc
     1261 tttgtggatc caccgcgctg gtgggctcgt cgagcagcag caattccggg cggtgcagcg
     1321 ttgccgcggc cagcgccagc cgctggcgct ggccgccgct catggtcccg gcacgctgtt
     1381 tcgccagccc cgccaggcgg tactcggtca tcgcctcggc cagcctggcg cgcttctcgc
     1441 gggcgggcag gccatgcact tcggcgacga aatccaggtt ctcacgcacc gacaggtcct
     1501 cgtacagcga gaaccgctgc gtcatgtagc cgatccggcg acgcacggcc tcggcctggc
     1561 cggggacctc gtagcccagc accgtgaccc tgccggcggt cggcaggagc aggccgcaca
     1621 gcaggcggat ggtggtcgac ttgcccgatc cgttcgggcc gagaaaaccg tagatggcgg
     1681 cacgcggcac gtcgaggtcc agcgcatcga ccgccaccag cgcaccgaag cggcgcgtca
     1741 gcccggtggc gacgatggcg gcgtcgccgc tcactgcgcc gccagcccca gcgcggggaa
     1801 gtccacctgg accggcaggc cggccggcag gtcgcgcgct gccggctcgg tcagcaccag
     1861 ttcggcgagg tagaccaggc gccctcggtc ccgctccgtc agggcgaaat acggcgtgaa
     1921 cgccgcctcc gcggagatcg tccggacggt ggcggcaaag acctgctcgt agccggccac
     1981 ccggacctcg gccgcagtgc cggcatgcac ccgggcgcgc accgcctggg gcacgtagac
     2041 gcgcgcatgg ggcgcctggt cggccagcat cacgatcacc accgcgcctg gctgggggcg
     2101 atcaccgcgc ttgtagggga tggcgtccac cacgccgtcg cgcggcgcgc gcaggacgag
     2161 gcgctcgacg gccaaccgcg catcggcgag caccgcctcg gcctcggcga gggccgccac
     2221 cgcctggtcc agttcctcga cggtcgtgcc ctccagcagg gcctccaccc gggcttccca
     2281 ctggcgccgt tcggcctccg cggcatcgcg ccgcagctgc gcaccgtcgc gctcggcggc
     2341 gctcaccagg ccccgctcga ccagcgcttg cagtcgctcg tactcgcgcc gctcgagagt
     2401 gagccgccgg cgggcgccgt ccagcccggc ctgcgcttcc gcgatcgatt cgcgacgcgg
     2461 gccgcgcagc agctcgtcga cgcggcgagc ggcgcgcgtg cgtgccgcct cggcgtgggc
     2521 caccgcggtc tgtcgccggg cagcgtcctg ccgcgccagg acctcgccgg cggctaccgc
     2581 cgcgccctcg cgggcgtgca tctccagcag cgtctcgtgc gcctccgcca cgatctctat
     2641 gcggtcccgc tccagcgtgc cgacggccgg cagtcccgtg ccgtcgccgc cgcaggctgc
     2701 cagcatcgtc agcaacaggc acagcgacag gcagcggttc atggccgggc gccccggtga
     2761 aacatgcgcg cagcatgcgc cgaccaacga gcggcaaagg cgatgtcgac cggagctgcg
     2821 aacagctgtt ctgccagcgg tcgtaccagg aacgggaaca ccgcggccga cagcagcgac
     2881 agcacgatca gatccgggtc cagtcccggc tcgatctccc cccgtcgctt cgcatgctcg
     2941 acggcgctgc gcagcaggtc cctcagccgg ctgggaaaac gccgcacgaa gtccgcgccc
     3001 ggcccgtcgc ccaggatcag ttccctgaac agcagcggcg ccacccaggg ttgtcgccca
     3061 agcagctccg tgtgccgcgc caggtacacg tcgaggccgc cggggccctc gatctcgtcg
     3121 cgcaccgcga gcatgctgcc gagcgcctct tcgaggtagt cctccagcac cgccagccac
     3181 aggccgtgct tgccgccgaa gtagtagtgc accatcgccg gggtgacacc cgcggcctct
     3241 gcgacctgtt tgagggagac ggcttcgaag ccgtgcctgg caaacagttc gcgggccgcc
     3301 gtgccgagac gcgcgcgcac ctccttgcgg ctctgctcgc tgggccggcc caccttgcgg
     3361 ttcctcgcga cgcggctcac acgactctgc ccgaccatcc tgcacctgcc cgatcacgac
     3421 ctgtgccata aattaattaa tgtggcaatt aatttcaagt cgcgggcaac gctatgctga
     3481 cgccgccctg ccggaggaag caccgtgagc aagatattcg ccgccctgct ggccctgacc
     3541 gttgcgctgc cttccgccct cgcccagccg cgagcggagc cggtgctgct gattcacggc
     3601 ggcgccggca cgctgcgcgc cgccgacctg agcgacgccg accgcgcgcg ctacgaggcc
     3661 gcactccacg aggccctgga cgccggctgg cgcgtgctgg ccggcggcgg cagcgcgctc
     3721 gacgcggtca ctgccgccat cctgccgatg gaggactcgc cgttgttcaa cgcagggcgc
     3781 ggcgcggtgt tcactgccga cgggcgcaac gagctggacg catcgatcat ggacggcgcc
     3841 acccgcgccg ccggcgccgt cggcggtgtg cgctcggtgc gccatccggt attgctggcg
     3901 cgtcgcgtga tggaggcctc gccgcatgtg ttcctggccg ggccgggtgc gctggagttc
     3961 gccatcgacc aggggctgga actcaagccg gccgagtggt tctggacgga gcgccgctgg
     4021 cagcagctgc aacgcgccac gctgcgcaat gagaccggcg cggcgcccgg cgacgcgggc
     4081 gacgaccgtt tcggcacggt cggggcggtc gcgctggatg ctgccggcca actcgccgcg
     4141 gccacatcga ccggcggcac cacggcgaag cgctacggcc gtctcggcga cgtaccggtc
     4201 atcggcgccg gcacctgggc ggacgcgggc tgcgccgtgt ccgccaccgg cgacggggaa
     4261 tacttcatcc gcgccgcggc ggcgcgttcg atttgcgcaa ggatggcatg gacgggcgca
     4321 gcgcccggcg atgcggcggc cgccgtgctg caggaaatca gcgcactggg cgggctcggg
     4381 ggcgtcatcg tggtggacgg cgcgggccgc ttcagcatgc tgttcagcac cgagggcatg
     4441 taccgcggcc acgccgacgc gcgcgggcgg caggtggcga tcttcgcccg ggactaggcc
     4501 ctgccctctt ttcgcgggtc gtccgcggca tggatcggcc gccgcttgag cccggatctc
     4561 aggcctggcg cgccagccag gcgatgtagg cgaagtaggc cgcaagcagc gccaggccct
     4621 ccggccggcc gatgcgttgc cgcaccatga tcagcggcac cagcatcagg gcaagcatca
     4681 gcatcaccac caggtcgacc atggtgatat cgccgcggtc caggggccgc agcacggccg
     4741 tgatgccgag gatggccagg atattgaaga agttcgagcc gatgacgttg ccgaccgcga
     4801 tgtcgtcgtt gcccttcagc gaggcgacga tgctggtggc cagttccggc aggctggtgc
     4861 ccacggcaat caccgtcagc gcgatcagcg ccgggctcac gccgagcgcg gtggcgagcc
     4921 ccacgccgcc gcgcacgaaa gcagcgccgc cggccaccag cgcgaccagc ccgccgacga
     4981 tgaacaccca gtccatgaac gccgattcct ggacccgcgg cgcgacgccc tcgaactcct
     5041 ggcggacccg ctcgacttcc tgctcccggc gcgccacctg gatgttccag tacaggaaca
     5101 cgccgaggcc ggcgagcaac accagcccgg cgggccggct gatgtggccg tcgttcagga
     5161 aagccaccag gagcagcgac acgaagatca gccagggcag gtcaagcgac aggatgcgcg
     5221 cctcgatctt cacgggccgc agcacggcgc agatgcccag gatcaggccg atgttggcga
     5281 tgttcgagcc gaccacgttg ccgatcgcga cgtcgtccac ccccgccagc gccgcctgca
     5341 ggctgaccgc cagctcgggc gcgctggtgg cgaaggccac gatcgtgatg ccgatgatga
     5401 acggactcat gccgagccgc agtgccatgg tgcccgcacc ccgcaccagc ccttcgccgc
     5461 ccgcgtagag cagtacggac cccagcacga ggaaaccgac gtgaacgatc atcccgcctc
     5521 cgccatcgcg aaataactcg ccaggaattc ctcgaacggc tcggccgggc ccgcttccat
     5581 cgcctgctgc gcctcgagcg aacgtgccgc ctcctgctcg aactcctgca gccgctcggg
     5641 cgacatcggc ggcagggcgg cgaagtactc ctggtgctgg cgcgacatgc gcagcgcaaa
     5701 ctggaagaac gactcgcctt cctggcgcat ctcggcgagg atgcgcgcgg agggcgtgag
     5761 cgacacgtcc tcgatcttcg cggcctgcag ctcgagcgcc gcgctgtagg agcgcaacgg
     5821 atcgttgccg tcgagcagcc gacagatggg gacgagctgc cgacagattt cgcgtcccca
     5881 ttcggccagc ggcttcttca cgcccgagat cgcgagcgcc aggccggggc aacgcccggt
     5941 gacggcgacg cgggcatggt tgtcgtcgag cagttcgagt tcgcctgcgg tgatcggcgg
     6001 gctgtcgtgc agcatgcaga aaatcaggaa ggcctccagg aagcggaatt cgttctggct
     6061 cacgccggcc ggatcatagg ggctaacgtc cagggcgcgc acttccatgt aggccacgcc
     6121 ggcgcggcgc agcgccagcg tcggccgctc gccggggcgg atcggctgct tgggacgaat
     6181 gaagccgtag tactcgttct cgatctgcag cgcgctggtc gagagctgcc gccactcgcc
     6241 ggccgacacc acgccgatct tctcgtaggg cggccacggc gaacggatgg cctgctcgag
     6301 gcccgcgacg tactcgtcga gcgtgttcat cgacaggtgg atactggcct ggctggtgtt
     6361 gcgatagccc aggtcgctca tcctcagcga ggtcgcgtgc gggccataga tcgttgcggg
     6421 atcgaactcc ggcaggccgg gatcgcggcc gcccatgaag gacttgcaga tcgccggcga
     6481 ggcgccgaac aggtagagca cgatccagcc gaagcggcgg aaattgcgca gcagcccgaa
     6541 gtacatccgg tcgcggaatt cccgttccgt gccctgccag ccctccacct cgcgaaacac
     6601 ctgccagaac ggctccggca gcgagtaatt gaaatgcaca ccggagatcg tctgcatgac
     6661 gcggccgtag cgatagccaa gcccgagccg gtagatgtgt ttcatccggc cgatgttgga
     6721 gctgccgtac tcggccaccg ggacgctggc gtcaccaccc accgcgcagg gcatgctggt
     6781 ggcccagagc atttcgtcgt cgggcagccg cggatagacg aactggtgga tgtcgcacag
     6841 gaactgcaag gtctcccagc agctttgctg cggcggcgtg acgaactcga gcagtgcctc
     6901 cgagtagtcg gtggtgatcc aggggtgggc gagcgcagac ccgagtgccc ggggatgggg
     6961 cgtcgctgcg atgcgcccgt tcggcgctac ccgcagcgac tctttttcaa cgccgcgacc
     7021 gccgccggac aggcagccgc ggtccaggtg cgcgaggc
//
LOCUS       DAOMPG010000115         7016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4614_length_7016_cov_3.872145, whole genome shotgun sequence.
ACCESSION   DAOMPG010000115 DAOMPG010000000
VERSION     DAOMPG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_7016_cov_3.872145"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLS34_07505"
     CDS             <1..330
                     /locus_tag="PLS34_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HRP87350.1"
                     /translation="LAKWQPYLFGIGTGLQAFFMMGAGTLGVSRRHWDMAFTGSAFEW
                     AYPGAAYTLMGLNGISAVIAVLGGALFGVIMVGTILFGKKKAGASEYGTRRPQWPRPW
                     AATARSA"
     gene            440..1369
                     /gene="cyoE"
                     /locus_tag="PLS34_07510"
     CDS             440..1369
                     /gene="cyoE"
                     /locus_tag="PLS34_07510"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRP87351.1"
                     /translation="MNARHDALPSPGQGAAALPTLPRSGLLRLCANLFKLRIGFAIVL
                     SALAGAILAQGDWPAGWDTVVLALGVLIASSGAAGCNHYFERDLDARMHRTRGRPFVS
                     GRLDVSALWPALFGAMMLFGSALVAWRFNLASGAFVLAGALTYSVVYTLWLKRRTHWN
                     IVIGGLSGSWAVLAGAAAFDGTLSPVALVLAVVLFLWTPSHFWSLAIALVQDYRRAGV
                     PMLPVVRGVEVASRWALINAVLLAGSALVLAAMLDSPFVWAGALLGGAWLCETHRRLV
                     QRPTPPVAMAAFRASLIQLGLLFAGLFIAYGLV"
     gene            1366..2229
                     /locus_tag="PLS34_07515"
     CDS             1366..2229
                     /locus_tag="PLS34_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP87352.1"
                     /translation="MKRCAASPARSSWRRTLAALIIVPVLVLAAAPATARTGVSPGAT
                     GIEARAIELSQAALGNEVGSLAFYDQRGERRSLEEFRGRPVVLSLIYTACVHSCQVAT
                     RQLDRVVRIARAAIGDDSFTVLTVGFDQPTDTPAQMAAYARRYGISDPHWHFLSSPDR
                     ATIDALMARVGFVYEPSPRGFDHTVKATILDAEGRVWRHVYGEVFPTPLLVEPLKDLV
                     WNRPVSAGGLMATIGDRVRLFCTVYDAQGDRYMFDYSLFAGIFIGVLVLGSALAWLLV
                     ELHRARRSRPA"
     gene            2226..3782
                     /locus_tag="PLS34_07520"
     CDS             2226..3782
                     /locus_tag="PLS34_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRP87353.1"
                     /translation="MSREHPGPNWVLRLLARFERPFSAAFAAQNNPFHHLGALTIYFF
                     WIILVSGIYLFLFYDTSVSGAWLSLERITHGQWYAGGVMRSLHRYASDAAIITMLLHL
                     SREFLRGRYRGVRWYSWFTGVPLLWLVFIFGMTGYWMVWDQLAQYVAVSTARLLDWLP
                     IFSDPMIRNFLTNETVNERFFTLMAFIHLVGLPIGLVLGVWFHLLRVRLPRIHPPRVL
                     MAGSLLTLLALSLAVPAVSHPPADLGLVPLDLRLDWFYLAVFPLLDLSSEGFVWAGLW
                     GISLLLCALPWLPPLKHPPAAEVHLPDCTGCEFCAEDCPYGAIEMVPRTDGRNFTLEA
                     KVDPALCVSCGICTGSCPSSSPFRRQAPLRTGIALPYFDMEEFKRRLDAAAAGARADG
                     PRIVLVGCDYSVAVEGLRQSGVAALSLPCAGMMPPAAIDYLLRIGGADGVVLTGCDAG
                     DCHFRHGDRWVEARVARERMPMLRGRVPRERLLLSWHKPVDAGGLRRAVDAFRERLAA
                     LPEDVAEQRR"
     gene            3779..4324
                     /locus_tag="PLS34_07525"
     CDS             3779..4324
                     /locus_tag="PLS34_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87354.1"
                     /translation="MKRMTAGMLAGQAVAGLLFASITGAFAQWPRYSPLPAGHGELKL
                     SMAHLTERLVPCVQLSAEELAALPPNMRNPERCERERAPAVIELRANDAVLLDTVVRP
                     VGLARGGRSYLQARWPLAAGDYELEIKLRDTPRAEGFDLVQRLGLTLRPAESALLELG
                     DGEARLIAGRDADAAAMETTP"
     gene            4321..5718
                     /locus_tag="PLS34_07530"
     CDS             4321..5718
                     /locus_tag="PLS34_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HRP87355.1"
                     /translation="MNAMNAMNKAGYRLEIPQDFRRHMAVAWLMLGVAGLLLSGLFVV
                     LIVASRTPGLQAVFPFQGFFHLAIVAHVDFSVLVWFAACGAMIWSLSTAPRAAGLGWA
                     ALGIAFAGAALMAVAPFQGGEAVMSNYVPVLDNPPFLAGLALFALGVVLMAGRALAFP
                     LPAGARLAPGGVLRFGSHTSAIAILLAAGALVWSLLTLPSYLTGPQYFEALFWGGGHV
                     LQFAWVQLLLVAWLWLAAASGLRMPLTPRLVTLLLLLGVLPAFLAIWAYLAHEPGGPG
                     HRTFFIWLMAAGGGVAAGPIALALLQGWRGSPRPASTTERGLRSALWFSILLMGIGGV
                     LGFMIDASNTMIPAHYHGCIVAVTLAFMSLSLHLLPRLGFAAPSPRLVLWMPRIYGIG
                     QILHVAGLAFSGGHGVQRKTAGAEQGLESLAQILGMSVMGLGGLIAITGGVIYLVAVA
                     GTLRRSLPMAGAHAA"
     gene            5741..5950
                     /locus_tag="PLS34_07535"
     CDS             5741..5950
                     /locus_tag="PLS34_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87356.1"
                     /translation="MLEILLLGLVVVVIYLVAHFLVTRAETMVGVPLGYWRTVMFFVV
                     FLGLLLAALELMPRLWPGGSPAGAP"
     gene            5947..6411
                     /locus_tag="PLS34_07540"
     CDS             5947..6411
                     /locus_tag="PLS34_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRP87357.1"
                     /translation="MIDDVALFLAHAVRLEGDAADTYERLAEQMEERANVSVAELFRK
                     FSGYCRQHLGEARERAVRETGSVPELAPEAYRWPDGYSPETARPGDLRKLTVRGALAL
                     ALDTERRACDFYAAVAGQTRSDAVQELAQEFAEEEAEHVEALERWLARLDQA"
     gene            6508..6774
                     /gene="rpsT"
                     /locus_tag="PLS34_07545"
     CDS             6508..6774
                     /gene="rpsT"
                     /locus_tag="PLS34_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRP87358.1"
                     /translation="MANIKSAQKRARQSVKNRAHNMAMRSKMRTYLKKVAAAIESGDR
                     AAAEASYQAAVPVLDSMVNKGIVHKNKAARHKSRLNTRIRSLQA"
BASE COUNT          879 a         2460 c         2460 g         1217 t
ORIGIN      
        1 ctggcgaagt ggcagcccta cctgttcggc atcggcaccg ggctgcaggc cttcttcatg
       61 atgggcgccg gcacgctcgg cgtatcccgc cgtcactggg acatggcctt caccggatcc
      121 gcgttcgagt gggcctaccc cggcgccgcc tacacgctga tgggcctgaa cggcatcagc
      181 gcggtgatcg cggtgctcgg cggcgcgctg ttcggcgtca tcatggtcgg caccatcctg
      241 ttcggcaaga agaaggccgg cgcgtcggag tacggcacgc gccgcccgca gtggccgagg
      301 ccatgggcgg ccacggctcg atcggcgtga agggcttcgc ggcgcccggc actttcatcc
      361 tcgcgatgct gctgctggtg atcttcgtcg tgtactactt catcaactac ggctacctgg
      421 ccacggtctg gaggctcagt tgaacgcccg tcacgatgcc ctgccctccc cggggcaggg
      481 cgccgcggcc ctgccgacgc tgccccgcag cggcctgctg cggctttgcg cgaacctctt
      541 caagctgcgc atcggcttcg cgatcgtgct ctcggcgctg gcgggagcca tcctggcgca
      601 gggcgactgg ccggccggct gggacaccgt ggtgctggcg ctgggcgtac tgatcgcctc
      661 ctccggtgct gccggctgca atcattactt cgagcgcgac ctcgatgcac ggatgcaccg
      721 cacccgcggc cggcccttcg tctcagggcg gctggacgtg tcggcgctct ggccggcgct
      781 gttcggcgca atgatgctgt tcggctcggc gctggtggcc tggcgcttca acctcgccag
      841 cggcgcgttc gtgctggccg gcgcgctgac ctactcggtg gtctacacgc tgtggctgaa
      901 gcgtcgtact cactggaaca tcgtcatcgg cggcctgtcg ggcagctggg cggtgctggc
      961 gggagcggcg gccttcgacg gcacgctctc cccggtcgcg ctggtgctgg cggtcgtgct
     1021 gttcctctgg accccctcgc acttctggag cctcgccatc gcgctggtcc aggactaccg
     1081 gcgcgccggg gtgccgatgc tgccggtggt gcggggtgtc gaggtcgcca gtcgctgggc
     1141 gctgatcaac gccgtgctgc tggccggcag cgccctagtg ctcgccgcga tgctcgacag
     1201 cccgttcgtc tgggccggcg cgctgctcgg cggcgcgtgg ctgtgcgaga cgcatcgccg
     1261 cctggtgcag cggccgacgc cgccggtcgc catggcggct ttccgcgcct cgctgatcca
     1321 gctcggactg ctctttgccg gcctgttcat cgcctacggc ctggtatgaa acggtgcgcc
     1381 gccagcccgg cacgatctag ctggcggcgc accctcgcgg cgctgatcat tgttcccgtg
     1441 ctggtcctgg ccgcggcgcc cgccaccgcc aggaccggcg tatcgcccgg tgcaacgggc
     1501 atcgaagccc gcgccatcga gctcagccag gcggcactcg gcaacgaggt ggggtcgctc
     1561 gccttctacg accagcgggg cgagcggcgc agcctggaag agtttcgcgg ccggccggtg
     1621 gtcctcagcc tgatctacac ggcgtgtgtg cattcctgcc aggtggccac ccgccagctc
     1681 gaccgcgtgg tccggatcgc ccgggccgct attggcgacg acagcttcac cgtgctgacg
     1741 gtcggcttcg atcagccgac ggacacgcct gcacagatgg cggcctatgc gcgccgctac
     1801 ggcatcagcg atccgcactg gcattttctc agcagcccgg accgcgccac catcgacgcg
     1861 ctgatggcgc gggtcggctt cgtctacgaa ccctcgccgc gcggcttcga ccataccgtg
     1921 aaggcgacga tcctcgatgc cgaggggcgg gtctggcgcc acgtctatgg cgaagtgttt
     1981 ccgacgccgc tgctggtcga gccgctcaag gacctggtct ggaaccggcc cgtctcggcc
     2041 ggcgggctga tggccacgat cggtgaccgc gtgcggctgt tctgcaccgt ctatgacgcg
     2101 cagggcgacc gctacatgtt cgactactcg ctgttcgccg ggattttcat cggcgtgctg
     2161 gtgctcggca gcgcgctggc ctggctgctg gtggaattgc accgcgcgcg ccggagcagg
     2221 ccggcatgag ccgcgagcac ccggggccga actgggtact gcggctgctg gcgcgtttcg
     2281 agcggccgtt ctcggccgcc ttcgcggcgc agaacaaccc cttccatcac ctcggcgcgc
     2341 tgaccatcta cttcttctgg atcatcctgg tcagcggcat ctacctgttc ctgttctacg
     2401 acaccagcgt gagcggggca tggctgtcgc tggagcgcat cacccacggc cagtggtacg
     2461 caggcggggt gatgcgcagc ctgcatcgtt acgcctcgga tgcggcgatc atcaccatgc
     2521 tgctgcacct gagccgcgag ttcctgcgcg gccgctaccg cggcgtacgc tggtactcgt
     2581 ggttcaccgg cgtgccgctg ctgtggctgg tgttcatctt cggcatgacc ggatactgga
     2641 tggtctggga ccagctggcc cagtacgtcg ccgtctcgac cgcccgtctg ctcgactggc
     2701 tgccgatctt ctccgacccg atgatccgca atttcctcac caacgagacg gtcaacgagc
     2761 gcttctttac gctgatggcg ttcattcacc tggtcggact gcccatcggg ctggtgctgg
     2821 gggtctggtt ccacctgctg cgcgtgcgcc tgccgcgcat ccacccgccg cgcgtcctga
     2881 tggccggcag cctgctgacg ctgctggcgc tgtcgctggc cgtgcccgcg gtgagccacc
     2941 cgcccgccga cctcgggctg gtgccgctgg acctgcggct cgactggttc tacctcgccg
     3001 tgttcccgct gctcgacctg agcagcgagg gtttcgtctg ggcgggcctg tggggcatct
     3061 cgctgctgct ctgcgccttg ccctggctgc cgccgctgaa gcacccgccc gcggcggagg
     3121 tgcacctgcc ggactgcacc ggctgcgagt tctgcgccga ggactgcccc tacggtgcga
     3181 tcgagatggt gccgcgcacc gacggccgca atttcacgct cgaggccaag gtcgatcccg
     3241 cgctgtgcgt cagctgcggc atctgcaccg gctcctgtcc ctcctcctcc ccgttccggc
     3301 gccaggcgcc gttgcgcacc ggcatcgcgc tgccctattt cgacatggaa gaattcaagc
     3361 gccgcctcga tgcagccgcc gccggcgcac gggccgacgg gccgcgcatc gtgctggtcg
     3421 gctgcgacta cagcgtagcc gtcgagggcc tccggcagtc cggcgtcgcc gcgctcagcc
     3481 tgccctgcgc cggcatgatg ccgcctgcgg ccatcgacta cctgcttcgc atcggcggag
     3541 ccgacggcgt ggtgctgacc ggttgcgacg cgggtgactg tcatttccgc cacggcgacc
     3601 gatgggtcga ggcacgggtg gcgcgcgagc gcatgcccat gctgcgcggc cgggtgccgc
     3661 gggagcggct gctgctcagc tggcacaagc ccgtcgacgc cggcggcctc cggcgcgcgg
     3721 tggatgcctt ccgcgagcgc ctcgccgcgc tgcccgaaga cgtcgcggag cagcgccgat
     3781 gaagcgcatg accgccggga tgctcgcggg ccaggccgtg gccgggctgc tgttcgcctc
     3841 gatcaccggc gcctttgccc agtggccgcg ctactcgccg ctgccggccg ggcacggcga
     3901 gctcaagctc agcatggctc acctcacgga gcggctggtg ccctgcgtgc agttgtcggc
     3961 ggaagagctg gccgccctgc cgccgaacat gcgcaacccg gagcgctgcg agcgcgagcg
     4021 cgcaccggcg gtgatcgagc tgcgcgccaa tgacgcggtg ctgctggaca ccgtggtgcg
     4081 gccggtcggc ctggcccgcg gcggcaggag ttacctgcag gcgcgctggc cgctcgcggc
     4141 cggagattac gagctggaaa tcaagctccg cgacacgccg cgagccgagg gtttcgacct
     4201 ggtgcagcgc ctgggcctga cgctccggcc cgctgaatcg gcgctgctgg aactgggtga
     4261 cggcgaggca cggctgattg ccggccgtga cgccgacgcc gccgcaatgg agaccacgcc
     4321 atgaacgcca tgaacgccat gaacaaggcc ggctaccgac tcgagattcc gcaggacttt
     4381 cgccgccaca tggcggtcgc ctggctcatg ctaggcgtgg ccgggctgct gctttccggc
     4441 ctgttcgtgg tcctgatcgt cgcctcccgc acgcccgggc ttcaggcggt gttccccttc
     4501 cagggattct tccacctggc gatcgtcgcg cacgtggatt tctccgtgct ggtgtggttc
     4561 gccgcctgcg gcgccatgat ctggagcctg tcgacggccc cgcgcgcagc cggcctgggc
     4621 tgggcggcgc tgggcatcgc gttcgcgggc gccgcgctga tggcggtcgc acccttccag
     4681 ggcggtgagg cggtcatgag caactacgtg ccggtgttgg acaacccgcc cttcctggcc
     4741 ggcctggcgc tgttcgcgct gggcgtggtg ctgatggccg ggcgcgccct ggccttcccg
     4801 ttgccggccg gcgcgcggct cgcgcccggc ggcgtgctgc gcttcggcag ccacacgtcc
     4861 gccatcgcca tcctgctggc ggccggcgcc ctggtctggt cgctgctgac cctgccgtcc
     4921 tatctcaccg ggccacagta tttcgaggca ttgttctggg gcggcggaca cgtcctgcag
     4981 ttcgcctggg tgcagctgct gctggtggcc tggctgtggc tggccgccgc cagcggcctg
     5041 cggatgccgc tcacgccccg cctggtcacc ttgctgctgc tgctcggcgt gctgccggcg
     5101 ttcctcgcga tctgggccta cctggcccac gagcccggcg gaccggggca ccgcaccttc
     5161 ttcatctggc tgatggcggc cggcggcggc gtggcggccg gcccgattgc gctggcgctg
     5221 ctgcagggct ggcgcggcag tccgcgaccg gcatcgacca ccgagcgcgg gctgcgctcg
     5281 gcactgtggt tctcgatcct gctgatgggc atcggcggcg tgctcggctt catgatcgac
     5341 gccagcaaca ccatgatccc ggcgcattac catggctgca tcgtcgccgt gacgctggcc
     5401 ttcatgtcgc tcagcctgca cctgctgccg cggctcggct tcgccgcgcc gagccccagg
     5461 ctggtgctgt ggatgccgcg catctacggg atcgggcaga tcctgcacgt cgccgggctg
     5521 gcgttctccg gcggccacgg ggtgcagcgc aagacggccg gggccgagca gggcctcgag
     5581 agcctcgcgc agattctcgg catgtcggtc atgggactag gtggtctgat cgccatcacg
     5641 ggcggcgtga tctacctggt tgcggtcgcc ggtacactta ggcgcagcct gccgatggcg
     5701 ggagcgcacg cggcctgaca cggcccgcgg ggaggacaag atgctggaga tcctgctgct
     5761 cggactggtg gtcgtggtga tctacctggt cgcgcatttc ctggttaccc gggccgaaac
     5821 gatggtcggg gtgccgctcg gctattggcg caccgtgatg ttcttcgtgg tgttcctcgg
     5881 gctgctgctg gcggcgctgg agctgatgcc gcgactgtgg cccggcggca gcccggcggg
     5941 cgcgccatga tcgacgacgt ggcgctgttc ctggcccacg cggtgcgcct ggagggggat
     6001 gcggcggaca cctacgagcg gctggcggag cagatggaag agcgcgccaa cgtcagtgtc
     6061 gccgagctgt tccgcaaatt ctcgggctat tgccgccagc acctcggcga agcccgcgag
     6121 cgtgccgtcc gcgagaccgg cagcgtgccg gagctggcgc cggaggccta ccgctggccc
     6181 gacggataca gcccggaaac ggcccggccg ggcgacctgc gcaagctgac tgtgcgcggc
     6241 gccctcgcac tggcgctgga caccgagcgg cgcgcctgtg atttctatgc cgccgtggcg
     6301 ggccagacgc gctccgacgc ggtgcaggaa ctcgcccagg agttcgcgga ggaagaggcc
     6361 gaacacgtgg aggccctcga gcgctggctg gcgcggctgg accaggcatg acgggttgac
     6421 aacaccacgc cgctgaacga cgatatgcgg cttcgcgcag cggcctgagg gcccggcgca
     6481 cattgcgatt caacgggagt cggtaccttg gcgaacatca agtcggccca gaagcgcgct
     6541 cgccagagcg tcaagaacag ggcccacaac atggcgatgc gctcgaaaat gcgcacctac
     6601 ctcaagaagg tcgcggcggc gatcgagtcc ggcgaccgtg ccgcggcgga agccagctac
     6661 caggccgccg tgccggtgct cgattcgatg gtcaacaagg gcatcgtcca caagaacaag
     6721 gccgcccgcc acaagagccg gctgaatacc cgcatcagga gcctgcaggc ctgaccggcg
     6781 gatcggcttc ggtggactga aaaaaaccgg cgcatgcgcc ggtttttttg tgcccgaacg
     6841 ccgtcagcgg ggcgacgccg gcggctggcc gccgtgggcc gccttgagct tgtccatcgc
     6901 ttcctgccgg gcctgcgcct gggtggcgag gaacttgccc accgcacgga ccagctcgtc
     6961 agtgccttcc ccggtcatcg ccgacacggt gagcaccggg cccttgtagt cgatcg
//
LOCUS       DAOMPG010000116         6982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4638_length_6982_cov_4.409846, whole genome shotgun sequence.
ACCESSION   DAOMPG010000116 DAOMPG010000000
VERSION     DAOMPG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_6982_cov_4.409846"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..2167)
                     /gene="katG"
                     /locus_tag="PLS34_07550"
     CDS             complement(8..2167)
                     /gene="katG"
                     /locus_tag="PLS34_07550"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRP87359.1"
                     /translation="MSNESKCPVMHGGNTASGRTNMEWWPNALNLDILHQHDSKTNPL
                     GQDFNYREELKKLDVAALKKDLRALMTDSQDWWPADWGHYGGLMIRMAWHAAGSYRVA
                     DGRGGGGTGNQRFAPLNSWPDNANLDKARRLLWPIKKKYGNKLSWADLIILAGNVAYE
                     SMGFKTFGFAFGREDIWHPEKDTYWGSEKEWLAPSDNPNSRYSGERDLENPLAAVMMG
                     LIYVNPEGVDGKPDPLKTARDVRVTFARMAMNDEETVALTAGGHTVGKCHGNGDAKLL
                     GPEPEAADVEEQGLGWNNHTKRGIGRDTVTSGLEGAWTTHPTRWDNGYFHMLLNHDWE
                     LKKSPAGAWQWEPVSIKEEDKPVDVEDPSIRYNPIMTDADMAMKMDPEYRKISERFYK
                     DPAYFDEVFARAWFKLTHRDMGPKARYIGPEVPAEDLIWQDPVPAGSTSYDVAAVKAK
                     IAASGLSISELVATAWDSARTFRGSDMRGGANGARIRLAPQKDWEGNEPKRLAKVLGA
                     LEPIAKASGASLADVIVLAGNVGVEQAAKAAGFDIEVPFAPGRGDASQAQTDVESFEP
                     LEPIHDGYRNWLKKDYVVSAEELLLDRTQLMGLTAHEMTALVGGMRVLGTNHGGTKHG
                     VFTDRVGALTNDFFVNLTDMANTWKPAGKNLYEIRDRKSGKVKWTATRIDLVFGSNSI
                     LRSYAEVYAQDDNKEKFAKDFVAAWTKVMNADRFDLA"
     gene            2401..3216
                     /locus_tag="PLS34_07555"
     CDS             2401..3216
                     /locus_tag="PLS34_07555"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HRP87360.1"
                     /translation="MNVSAACPNCDTPLQGRYCHACGQKRIEPEERRLSWFVRKLVKE
                     VTMVDGRLLGSLGRLMFRPGELERDWLAGRRRRHLAPLSLFLIANLVYFFHPPLTDLN
                     MSLAEQVQLQPWSPLAERMVDSRLQSRGVGFDDYATEYRVKATELAKLMVILHVPLLA
                     LVLMWLHPRRGLHYVDHLAVSLNFWAFLLFMIMVVPWVLALLLTIVGLGSHGILQLTL
                     LGIVCVYAWQQMRLAYDQPGGRALAKLPVFILGFGVAHMAYRATQFLVAFALS"
     gene            complement(3256..5217)
                     /locus_tag="PLS34_07560"
     CDS             complement(3256..5217)
                     /locus_tag="PLS34_07560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRP87361.1"
                     /translation="MSKRFAYGAVLALCLGSPAAAVGALPLEDMVRQAEFTGASLSPD
                     GRHLALATPVDDRTCLTVLNIEDSRQLSVVLQSCPPGGESVLASGMLWPNDERILFRT
                     TQQVGNLAQPVLTGRIYAIGIDGKGGRQIHGPALGAMNFVYTSVSIIDMLPDDPEHIL
                     VSTLTYAMHGERLKPRVERVNVYNGRTSFVLDSPLARGGVVTDQQSRVRFATGYSVDG
                     EQSLAWRRDEDSDWIEFPSPFEADIWPLSFTADEKGILVASRQEGELGVWRIDLESGE
                     RRRLLVSERSDVRDAIYDARGKQLIGARFEPDYPAYEFIDPEHEDTMLWRRVLAAFPG
                     RQGSIEGFTRDRSRAVVEVTSDREPFAWYLLNTEDMQLRFLLSAQGWIDPKQMSAREP
                     FRIRARDGLELTGYLTLPPDGRDANLPAVMLVHGGPHGPHDTWSFELEAQLLASRGYA
                     VVQLNFRGSGGYSQQFERAGHRKWGTAMQDDVTDATRWLISEGIADPERICIYGASYG
                     GYAVLAGITREPDLYACAFTYVGVYDLDMMFRHGDIPQRPEGEAYLTRVLGDRRADAA
                     DIAARSPVNHVAKITTPLYVAHGYRDERVPMAQFNALTRALDRAGIPYEELVLRTEGH
                     SLADPENRIKFYTELLNFLDKHTGGAAGR"
     gene            5364..6740
                     /gene="mltF"
                     /locus_tag="PLS34_07565"
     CDS             5364..6740
                     /gene="mltF"
                     /locus_tag="PLS34_07565"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HRP87362.1"
                     /translation="MRILATIVLCLLLGTCSPHLTVLEQVQRHGVLKVVTRNAPTTYY
                     IGPEGPVGPEVELARGFADFLGVRLEIYALDNIARIIPEVTQGRAHVAAAALTATPSR
                     EATLEFGPTYRTVTQQLVYRQGTSMPRTVEQTYGRRLEVAAGSSFVETLEALRMEAPG
                     LTWLENPLRDVQELLALVANGEIDLTVADSTAVEINRFFYPDIRAAFDLSEPKPVAWA
                     FRAGDDHSLIEAARRYFDEIEANGRLAEITDRYLGHTDRFDYVGTRTFLQHIDTRLPR
                     YREWFEEVAAEHGMDWRLLAAISYQESHWNPRAVSVTGVRGLMMLTLRTAEAVGVTDR
                     EDPEQSIRGGARYLERVMEKIPERIPQPDRTWMALASYNVGFGHLEDARRITERRGLD
                     PDRWVDVRDSLPLLTQERWYKQTRFGYTRGWEPVQFVDNIRRYYEVLAWITADTFDRP
                     VVADGSRR"
BASE COUNT         1043 a         2333 c         2349 g         1257 t
ORIGIN      
        1 actcgcctca ggcgaggtcg aagcgatccg cgttcatcac cttggtccag gccgcaacga
       61 agtccttggc gaacttctcc ttgttgtcgt cctgcgcata gacctcggca taggaacgga
      121 ggatcgagtt cgagccgaac accaggtcga tccgcgtagc ggtccacttg accttgcccg
      181 acttgcggtc gcggatctcg tagaggttct tgcccgccgg cttccaggtg ttcgccatgt
      241 cggtgaggtt gacgaagaag tcgttcgtca gcgcaccgac gcgatcggtg aacacaccgt
      301 gcttcgtgcc gccgtggttg gtgcccagca cccgcatgcc gcccaccagc gccgtcatct
      361 cgtgcgccgt gaggcccatc agctgggtgc ggtcgagcag cagttcctcg gcactgacca
      421 cgtagtcctt cttcagccag ttgcggtaac catcgtggat gggttcgagc ggctcgaagg
      481 actcgacgtc ggtctgcgcc tggctggcgt cgccgcggcc cggcgcgaac ggtacctcga
      541 tgtcgaagcc cgccgccttg gccgcctgct ccacgccgac gttgccggcc agcacgatga
      601 cgtccgcgag gctggccccg ctcgccttgg cgatcggctc cagcgcgccg agcaccttgg
      661 ccagccgctt gggctcattg ccctcccagt ccttctgcgg ggccaggcgg atgcgcgcgc
      721 cgttggcgcc gccgcgcatg tccgagccgc ggaaggtccg ggcgctgtcc caggccgtgg
      781 cgaccagctc gctgatcgac agaccgctgg cggcaatctt cgccttcacg gccgcgacgt
      841 cgtagctggt gctgcccgcc ggcaccggat cctgccagat caggtcctcg gccggcactt
      901 ccggcccgat gtagcgcgcc ttcgggccca tgtcgcgatg ggtcagcttg aaccatgcgc
      961 gggcgaagac ctcgtcgaaa taggcggggt ccttgtagaa gcgctccgag atcttgcgat
     1021 actcgggatc catcttcatc gccatgtcgg cgtcggtcat gatcgggttg tagcggatcg
     1081 acgggtcctc gacatcgacc ggcttgtctt cttccttgat ggacaccggc tcccactgcc
     1141 aggcgccggc cgggctcttc ttcagctccc agtcgtggtt gagcagcatg tggaaatagc
     1201 cgttgtccca gcgggtcgga tgggtggtcc aggcgccctc caggccgctg gtcacggtgt
     1261 cgcgaccgat gccgcgcttg gtgtggttgt tccagcccag gccctgttcc tcgacgtcgg
     1321 ccgcctcggg ctccggtccg agcagcttgg cgtcgccgtt gccgtggcac ttgcccaccg
     1381 tgtggccgcc ggcggtcagg gcgacggttt cctcatcgtt catcgccatg cgcgcgaagg
     1441 tcacgcgcac atcgcgcgcg gtcttcagcg ggtccggctt gccgtcgacg ccttccgggt
     1501 tgacgtagat gaggcccatc atcaccgcgg ccagcgggtt ctcgaggtca cgctcgccgg
     1561 agtaacggct gttcgggttg tcggagggcg ccagccactc cttctcggag ccccagtagg
     1621 tgtccttctc cggatgccag atgtcctcgc ggccgaaggc gaagccgaag gtcttgaagc
     1681 ccatcgactc gtaggccacg ttgccggcga ggatgatcag gtcggcccag ctgagcttgt
     1741 tgccgtactt cttcttgatc ggccacagca ggcgacgcgc cttgtcgagg ttggcgttgt
     1801 cgggccagga gttcaggggt gcgaagcgct ggttgccggt gccgccgccg ccgcggccgt
     1861 cggccacccg gtaggaaccc gcggcatgcc atgccatgcg gatcatcagc ccgccgtagt
     1921 ggccccagtc ggccggccac cagtcctggc tgtcggtcat cagcgcgcgc aggtctttct
     1981 tcagcgcggc gacgtcgagc ttcttgagct cttcacgata gttgaagtcc tgccccagcg
     2041 ggttggtctt gctgtcgtgc tgatggagga tgtcgaggtt cagcgcgttg ggccaccact
     2101 ccatgttggt ccttccggag gcggtgttgc caccgtgcat caccgggcat tttgattcgt
     2161 tcgacatggc tattcacctt cgtgggcgga tgtgttcgtg agcgtgagtg gagtttagga
     2221 gacggctgcg gcataggtct aatgaattgc ggcaatccgg tcgatagttt ttgactatca
     2281 cgccgtagtg aagttcaatt tgtccgaaaa gcggagtttt tcaccgagct gagtcaatcc
     2341 actcggctgg aagtcgggca gcgctttgca gatacccgcc ccgagccgat aatcccggcg
     2401 gtgaacgtat cggccgcctg ccccaactgc gacacccccc tgcagggccg ctattgccat
     2461 gcctgcggcc agaagcgcat cgagccggag gagcggcgcc tgagctggtt cgttcgcaag
     2521 ctggtcaagg aagtgaccat ggtggacggc cgcctgctgg gcagcctcgg gcggctgatg
     2581 ttccggcccg gcgagctgga gcgggactgg ctggcgggac gccggcggcg gcacctcgcg
     2641 cccctgtcac tgttcctgat cgccaacctg gtctatttct tccacccgcc gctgacggac
     2701 ctgaacatgt cgctcgccga gcaggtccag ctgcagccct ggagcccgct ggccgagcgc
     2761 atggtcgact cccggctgca gtcgcgcggc gtcggctttg acgattacgc gacggagtac
     2821 cgggtcaagg ccaccgagct ggccaagctg atggtgatcc tgcacgtgcc gctgctggcg
     2881 ctcgtgctga tgtggctgca tccgcgccgc gggctgcact acgtggacca cctcgcagtc
     2941 agcctgaact tctgggcctt cctgctgttc atgatcatgg tcgtgccgtg ggtgctggcg
     3001 ctgctgctga ccatcgtcgg tctaggctcg cacggcatcc tgcagctgac gctgctcgga
     3061 atagtctgcg tctacgcgtg gcagcagatg cgcctggcct acgatcagcc gggcggccgg
     3121 gcgctggcca agctgccggt gttcatcctc ggcttcggcg tcgcgcacat ggcttaccgc
     3181 gcgacccagt tcctggtcgc cttcgcgctc agctgagacg gcgcccggtc gttttgcgac
     3241 cgggcgccgc gtggcctagc ggccggccgc gccgccggtg tgcttgtcga ggaagttcag
     3301 cagctccgta tagaacttga tgcggttctc cggatccgcg aggctgtgac cctcggtgcg
     3361 caggacgagc tcttcatagg gaatccccgc ccggtccagc gcgcgggtca gcgcattgaa
     3421 ctgcgccatc ggcacgcgct cgtcccggta gccgtgggcg acgtacagcg gcgtcgtgat
     3481 cttcgccacg tggttgaccg gggagcgggc ggcgatatcc gcagcatcgg cgcgacggtc
     3541 gcccagcact cgcgtcaggt aggcttcgcc ctccgggcgc tgcggaatgt cgccgtgccg
     3601 gaacatcatg tcgaggtcat agacgccgac gtaagtgaag gcacaggcgt acaggtcggg
     3661 ctcccgcgtg atcccggcga gcaccgcata gccaccatag ctggcgccgt agatacagat
     3721 ccgctccggg tccgcgatgc cctcgctgat cagccagcgg gtggcgtcgg tcacgtcgtc
     3781 ctgcatggcc gtaccccact tgcggtggcc ggcgcgctcg aactgctggc tgtagccgcc
     3841 ggaaccgcgg aagttcagct gcacgaccgc atagccccgg ctggccagca gctgggcctc
     3901 gagctcgaag gaccatgtat cgtgcgggcc gtgcgggcca ccgtggacca gcatcaccgc
     3961 cggcaggttg gcgtcgcgcc cgtccggcgg cagggtcagg tatccggtca gttcgaggcc
     4021 gtcgcgcgcg cggatgcgga acggttcgcg cgcagacatc tgtttcgggt cgatccagcc
     4081 ctgggcggag agcaggaatc gcagctgcat gtcctcggtg ttgagcaggt accaggcgaa
     4141 cggctcgcgg tcgctggtca cctccaccac ggcccggctg cgatcgcggg tgaagccttc
     4201 gattgacccc tgccggccgg gaaacgccgc cagcacgcgc ctccagagca tcgtgtcctc
     4261 gtgctcggga tcgatgaact cgtacgccgg gtagtccggc tcgaaccgtg cgccgatcag
     4321 ctgtttgcca cgggcgtcgt agatggcgtc ccgcacgtcg gagcgctcgc tgaccagcaa
     4381 ccggcggcgt tcgccggact ccaggtcgat gcgccacacg ccgagttcgc cctcctggcg
     4441 agacgccacc aggatgccct tttcgtcggc agtaaaggac agcggccaga tgtcggcctc
     4501 gaaggggctg ggaaattcga tccagtccga gtcctcgtca cgtcgccagg ccaggctttg
     4561 ttcgccgtcc accgagtagc cggtggcgaa gcgcacgcgc gactgttggt cggtaacaac
     4621 gccgccacgc gccagcgggc tgtcgagtac gaagctggtg cgcccgttgt agacgttgac
     4681 gcgctccacg cgcggcttca gccgctcgcc atgcatggcg taggtcaaag tcgacaccag
     4741 gatgtgctcc gggtcgtccg gcagcatgtc gatgatgctg acgctggtat agacgaagtt
     4801 catcgcacca agcgcgggcc cgtgaatctg ccggccaccc ttgccgtcga tgccgatggc
     4861 atagatgcgc ccggtgagca ccggctgcgc gaggttgccg acctgctgcg tggtccggaa
     4921 caggatgcgc tcgtcgttcg gccacagcat ccccgacgcc agcacggact cgccgcccgg
     4981 cgggcaagac tgcagcacga ccgacagctg ccggctgtcc tcgatgttca gtaccgtgag
     5041 gcaggtccgg tcgtcgaccg gcgtcgcaag ggcaagatgc cggccatcgg gcgacaggga
     5101 cgcaccggtg aactccgcct ggcgcaccat gtcttccaat gggagcgcgc cgacggccgc
     5161 cgccgggctc ccgaggcaca gggcgagcac cgcaccgtat gcaaagcgct ttgacatatt
     5221 cgcgtaacct catgaactcg tggaccggcc gcagcatgca ccgctgcggc gatgtcggcg
     5281 ggcaagctta tcatcttcgt gcggctggct gggttacggc ggctgcggca gacttgcacc
     5341 gctagcttct acgggacgga ctattgcgaa tcctcgccac catcgtgctt tgcctcttgc
     5401 tgggcacctg cagcccgcac ctcacggtgc tcgagcaggt gcagcgccat ggtgtgctca
     5461 aggtggtcac gcgcaatgcc ccgacgacct actacatcgg cccggagggg cccgtcgggc
     5521 cggaggtcga gctggcgcga ggcttcgccg atttcctcgg cgttagactc gagatctacg
     5581 cgctggacaa tatcgcgcgg atcattccgg aggtcacaca gggccgggcc catgtcgctg
     5641 ccgccgcgct gaccgcgaca ccgtcccgcg aggccacgct ggagttcggc ccgacctacc
     5701 gcacggtcac gcagcaactc gtttatcggc agggcacctc catgccgcgc acggtcgagc
     5761 agacctacgg ccggcgtctg gaagtggccg ccggcagcag tttcgtggaa accctcgagg
     5821 cgctgcgcat ggaggcaccg ggcctcacct ggctggagaa cccgctgcgc gacgtgcagg
     5881 aactgctggc gctggtggcg aacggcgaga tcgacctgac ggtggccgac tccaccgccg
     5941 tggagatcaa ccgcttcttc tatccggaca tccgcgcggc cttcgacctc agcgagccca
     6001 agcccgttgc gtgggccttt cgcgccggcg acgaccacag cctgatcgaa gccgcgcgac
     6061 gctacttcga cgagatcgag gccaatggac ggctggccga gatcaccgac cgctacctgg
     6121 gccacaccga ccgcttcgac tacgtgggca cgcggacctt tctgcagcac atcgacacgc
     6181 gcctgccccg ctaccgcgaa tggttcgagg aggtggccgc cgagcatggc atggactggc
     6241 ggctgctggc ggccatcagc taccaggaat cgcactggaa cccccgggcc gtgtcggtga
     6301 ccggcgtgcg cggactgatg atgctgacgc tgcgcacggc cgaagcggtc ggcgtgacag
     6361 atcgcgagga tcccgagcag agcatccgcg gcggcgcgcg ctacctcgag cgcgtgatgg
     6421 agaagattcc ggagcgcatc ccgcaacccg accgcacctg gatggcgctg gcttcctaca
     6481 atgtcggctt cggccacctc gaggatgccc gccgcatcac cgagcggcgc ggcctcgacc
     6541 cggaccgctg ggtggacgtg cgcgacagcc tgccgctgct gacgcaggag cgctggtaca
     6601 agcagacccg cttcggctac acgcgcggct gggagccggt gcagttcgtc gacaacatcc
     6661 ggcgctacta cgaggtgctg gcatggatca ccgccgatac cttcgaccgg ccggtggtcg
     6721 cggacgggtc gcggcgctag cgccgccggg cttttcgttc gggtcgaccg cggggacgcg
     6781 ccgactgctg cccggctcgg ccgagttgcc agaaggctgg ccgaccgttg cgcggcgtgg
     6841 cagatgtcgc catggcttgc ggccactgcc atgctcagcg gcagtgcgga ggcggggcgt
     6901 tgccatcgtc gctttcgcag cgggtggggg gtgtcatagg ccggccgagc gagtttttcc
     6961 ttgtccgagc gcaggccggc ct
//
LOCUS       DAOMPG010000117         6959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4653_length_6959_cov_3.404838, whole genome shotgun sequence.
ACCESSION   DAOMPG010000117 DAOMPG010000000
VERSION     DAOMPG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4653_length_6959_cov_3.404838"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLS34_07570"
     CDS             <1..850
                     /locus_tag="PLS34_07570"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRP87363.1"
                     /translation="NGALEQAINQLEQAAAAVEFDGLVEALHAAGVQTVVFEDTARPA
                     TPDSIFPNNWLSLHADGTAVLYPMMAPNRRGERRRDLLERLSREHGFRVARVLDLSGH
                     EQRERFLEGTGSMVLDRVNRIAYACLSPRTDLEVLAEAAQALGYEPLAFAASDANGMP
                     IYHTNVMMCIGADFAVLCEAAIRDAGQRAAVRRRLADTGHEVIPISLEQMACFAGNML
                     ELAGAGGERLLALSARALEVLDAPQRAALEVRCRLVAAPIAAIEEGGGGSVRCMLAEI
                     HLPRRA"
     gene            927..1757
                     /locus_tag="PLS34_07575"
     CDS             927..1757
                     /locus_tag="PLS34_07575"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRP87364.1"
                     /translation="MTPITRILMCPPDHFEVDYVINPWMDGNTGSPDPALARQQWERL
                     RSALAECARVELLEPRPGLPDLVFTANAGFVHGERAVPSHFMPHERRPEEPVLRAWFE
                     SQGFDVKVLPDNIAFEGAGDALIDRGGPWLWAGYGFRTEIEAHDYLRRWFDLEVVSIR
                     LVDPRFYHIDTCMCPLEGGYLLYHPPAFDDASRAEIERRVPRDKLIPVNDANAGHFAC
                     NAVNIGERIFLNRAGERLTAALEARGFRVTELGLSEFLKAGGAAKCLTLKLTEPAGHG
                     "
     gene            1831..3204
                     /locus_tag="PLS34_07580"
     CDS             1831..3204
                     /locus_tag="PLS34_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRP87365.1"
                     /translation="MASLPRVCVIGAGSSGIAVVKALQDRAIPQLCYEKGDDIGGNWY
                     FRNTNRMSAAYESLHINTDSRLMEYRDYPMPPGTPDYPSHQVIKRYFDDYVSHFGLRR
                     HIRFNTEVKRVVRRDDGRWEVSLDTGETEPFDVLVVANGHHWDPLWPDPPYPGEFLGE
                     QIHSHAYLNPREPVDFHGKRVLVVGMGNSAMDIACELSRPGIAEQLFLSARHGVWILP
                     KYLFGVPMSRLGLLPHWVPWWLGSVLTRLAVTVNAGPPWRFGLPRPDHRMLQAHPTIS
                     QDIYMKLGGGDVLPRPGIERLDGDAVVFTDGRREAVDVIVWCTGYKLSLPFFDPGFIS
                     APGNDLPLWERMVKPGVDNLFFVGLLQPLGAIMPIAEAQGHFIGDHLAGRIALPAVAA
                     MQHAMERERAAMFRRYKDRATRHTMQVDFAAYLHRLAGHARRGRRIAQRRDGWLPVPA
                     SRTTDLF"
     gene            3208..4116
                     /locus_tag="PLS34_07585"
     CDS             3208..4116
                     /locus_tag="PLS34_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRP87366.1"
                     /translation="MALRRNLARWLVRGVIRPGIAPGRSLARRRQHVERSARLLPPPR
                     GVRRAPAGAENPGGEWWLPPGVAPDTERGAVLYIHGGGFMLGSPASHRGVAARLARQT
                     GLPVLAAHYRLAPEHAYPDGLEDLQLAWRRITRGGARKVALAGDSAGGWLALALAHYA
                     AAAGLPRPFGAALFSPLVDLSGAAHIAPAAHDLMLPPGFVAESIAAWRGGLPADDPRF
                     DLLGAQLAELPPLFISHDRDELLAGDARRLARAAQAAGVQVRVEEGRGLWHAWPLLAG
                     LLPEADATLRSAAAMLTPAVLPGGPG"
     gene            4116..4988
                     /locus_tag="PLS34_07590"
     CDS             4116..4988
                     /locus_tag="PLS34_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP87367.1"
                     /translation="MPSASPTLRLLCGAAMISFAPVFVRLTPVSPTDSAFYRTLFGGL
                     MLAAWVIAARTPVERRSGLALGALIAAGVLFAADLAVWHRSIWYVGPGLATLLGNFQV
                     FLLAFVGILFFREKARWELLLAIPMAVVGLGMIVGFEWSTLDEQYRWGIVFGLLTAVF
                     YAGYILALRRARAASPAGSIVSDLMIVSLVSAFFLFAGAGAAGAPVELPDARSAWLLA
                     AYALVAQVLGWLLISGSLPHVPASRVGLILLLQPTLAFCWDVLIFDRAFGAREALGAV
                     LAITAIGLGSRPRR"
     gene            complement(4995..5888)
                     /locus_tag="PLS34_07595"
     CDS             complement(4995..5888)
                     /locus_tag="PLS34_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRP87368.1"
                     /translation="MPESVIVLANPAAGSGRRRRQGARAVALLERHGVPVETIVSRGA
                     GHLAEAAATEAARGRVRLVAIGGDGTLSEVARGLLSVPGHVTALGIVPLGTGNDFVKS
                     AGLPRSWRAACRRLADGCTPRSIDAGRVNGRWFINGVGFGFDAAIAQATLRHKWMPGP
                     LGYGAGLVEALWAGVDRPVCTLRWDGGEDRRAVTLVAACNGQFAGGLFRLAPRAELDD
                     GRLDIVWADALSRLQVLRHAPRVIRGTHESLPIAHFARSTRLEIVCDRPLPAQADGEL
                     LGADLVRLEIVLAAGVLRLWT"
     gene            6003..6641
                     /gene="plsY"
                     /locus_tag="PLS34_07600"
     CDS             6003..6641
                     /gene="plsY"
                     /locus_tag="PLS34_07600"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRP87369.1"
                     /translation="MPEILLKLVLGYLAGSVVGSLVVGRVRGIDIRNLGSGNAGGTNA
                     LRTQGKGFALAVVLIDVLKVVLPVVFLTGAALPGVGVDAAIARETVQVAIATGAVLGH
                     VWPLYFGFRGGKGMATLLGAYGAIQPALLGVVVLAWALLFVLRGYVGLATLFAAAMAP
                     VWLAATGQSGRPLFWFALLMAGFVAWTHRSNIARMRDGKETRMFPGLLGRRR"
     gene            6777..>6959
                     /locus_tag="PLS34_07605"
     CDS             6777..>6959
                     /locus_tag="PLS34_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HRP87370.1"
                     /translation="MLLDGDAIRQGLPAGVARRVGLLEVLPETASTNARVLAAERPVG
                     ELGVCLAEYQSAGRGRR"
BASE COUNT          873 a         2540 c         2459 g         1087 t
ORIGIN      
        1 aaacggcgct ctggagcagg cgatcaacca gctcgagcag gcggccgccg ccgtcgagtt
       61 cgacggcctg gtcgaggcgt tgcacgcggc cggcgtgcag accgtggtgt tcgaggacac
      121 cgcccgcccg gcgacccccg actcgatttt tcccaacaac tggctgagcc tgcacgccga
      181 cggcaccgcc gtcctctatc ccatgatggc gccgaatcgc cgcggcgaac gccgtcgcga
      241 cctcctcgag cggctcagcc gcgagcacgg ctttcgcgtg gcgcgggtgc tggacctctc
      301 cggccacgaa cagcgggagc gcttcctcga gggcaccggc agcatggtgc tcgaccgggt
      361 caaccgcatc gcctatgcct gcctgtcgcc gcgcaccgat ctcgaggtgc tggccgaggc
      421 ggcccaggcg ctgggctacg agccgctggc gttcgccgcg tcggacgcca acggcatgcc
      481 gatctaccac accaacgtca tgatgtgcat tggcgccgac ttcgcggtgc tctgcgaggc
      541 ggccatccgc gacgccgggc agcgcgccgc ggtgcgacgg cgcctggcgg acaccggcca
      601 cgaggtgatc ccgatcagcc tcgagcagat ggcctgcttc gccggcaaca tgctggagct
      661 ggcgggggcg ggcggcgagc ggctgctggc cctctcggcg cgcgcgctgg aggtgctgga
      721 cgcaccgcag cgcgcagcgc tggaggtgcg ctgccggctg gtggcggcgc cgatcgccgc
      781 cattgaggag ggcggcggcg gcagcgtgcg ctgcatgctg gcggagatcc acctgccgcg
      841 gcgcgcctga gtgaccgccc cgcgagattt ttgcacataa tatgcagtag actcggcgcc
      901 cgtcccgact cgagcccggt gcctgcatga cccccatcac ccgcatcctg atgtgcccgc
      961 ccgatcattt cgaggtggat tacgtcatca acccgtggat ggacggcaac accggatcgc
     1021 cggatccggc gctcgcccgc cagcagtggg aacgcctcag gagcgccctc gccgagtgcg
     1081 cccgggtgga gttgctggag ccccgacccg ggctgcccga cctcgtgttc accgccaacg
     1141 ccggcttcgt gcacggcgag cgcgccgtgc cgtcgcactt catgccgcac gagcgtcgtc
     1201 cggaagagcc ggtgctgcgg gcctggttcg agtcgcaggg cttcgacgtc aaggtgctgc
     1261 ccgacaatat cgccttcgag ggcgccggcg atgcgctgat cgaccgtggc ggtccgtggc
     1321 tgtgggccgg ctacggcttc cgcaccgaga tcgaggccca cgactacctg cgccgctggt
     1381 tcgacctcga ggtggtgtcg attcgtctcg tcgacccgcg cttctatcac atcgacacct
     1441 gcatgtgccc gctcgaaggc ggttacctgc tgtatcaccc gccggctttc gacgacgcgt
     1501 cgcgcgcaga gatcgagcgg cgggtgccgc gcgacaagct gatcccggtg aacgacgcga
     1561 atgcgggcca tttcgcctgc aatgccgtca acatcggtga gcgcatcttc ctcaaccgcg
     1621 ccggtgagcg gctcacggcc gcgctcgagg cgcgcggttt ccgcgtcacc gagctcggcc
     1681 tgtcggaatt cctcaaggcc ggcggcgccg cgaagtgcct gacgctgaag ctgactgagc
     1741 ccgccgggca cggctgagcg cgggagcgcc tccgcggtcg ccgccgcgcg gatagaatag
     1801 gcgtccagac ctacgcccgg agtccacggc atggcctcac tgccccgcgt ctgcgtcatc
     1861 ggtgccggtt cgagcggcat cgccgtcgtc aaggcgctgc aggaccgcgc catcccgcaa
     1921 ctctgctacg agaagggcga cgacatcggt ggcaactggt atttccgcaa taccaaccgc
     1981 atgtcggccg cctacgagtc gctgcacatc aacaccgact ccaggctgat ggagtaccgc
     2041 gactacccga tgccgccggg gacgccggac tacccgagtc accaggtcat caagcggtat
     2101 ttcgacgact acgtaagcca tttcgggttg cgcaggcaca tccgcttcaa caccgaggtc
     2161 aagcgcgtgg ttcgccgtga cgacggccgc tgggaagtgt cgctcgacac gggggagacc
     2221 gagccgttcg acgtcctggt ggtcgcgaac ggccatcact gggacccgct ctggcccgat
     2281 ccgccctacc cgggcgaatt cctcggcgag cagatccatt cgcatgccta cctcaacccg
     2341 cgcgagccgg tcgacttcca cggcaagcgc gtgctggtgg tgggcatggg caacagtgcc
     2401 atggacatcg cctgcgagct gtcgcggccg ggcatcgctg agcagctgtt cctgtcggcc
     2461 cgtcacggcg tctggatcct gccgaagtac ctgttcggcg tgccgatgtc gcgtctcggc
     2521 ctgctgcccc actgggtgcc gtggtggctc ggcagcgtgc tgacgcgcct ggccgtcacc
     2581 gtcaatgccg gcccgccctg gcgcttcggc ctgccgcgcc ccgaccaccg gatgctgcag
     2641 gcgcatccga ccatcagcca ggacatctac atgaagctcg gcggcggcga cgtcctgccg
     2701 cgcccgggca tagagcgcct ggacggcgat gcggtggtct tcaccgacgg tcgccgcgaa
     2761 gcggtcgacg tgatcgtctg gtgcaccggc tacaagctca gcctgccgtt cttcgacccc
     2821 ggtttcatct cggctcccgg caacgacctg ccgctttggg agcgcatggt gaagccgggc
     2881 gtcgacaacc tgttcttcgt cggcctgctg cagcccctcg gcgccatcat gccgattgcc
     2941 gaagcccagg gacattttat cggcgaccac ctggcgggcc gcatcgcgct gcccgccgtc
     3001 gccgcgatgc agcacgccat ggagcgcgag cgcgccgcca tgttccgccg ctacaaggac
     3061 cgcgccaccc gccacaccat gcaggtggat ttcgcggcct acctgcaccg gctggcgggt
     3121 cacgcgcgcc gcggccggcg cattgcgcag cgccgcgacg gttggctgcc ggtgcctgcc
     3181 agtcgcacca cggacctgtt ctgagccgtg gcgctgcgac gcaacctggc gcgctggctg
     3241 gtgcgcggcg tcatccggcc cggcatcgca ccgggccggt cgctggcacg ccggcggcaa
     3301 cacgtggagc gcagcgcgcg cctgctgccc ccgccccgcg gcgttcgccg cgcgccggcc
     3361 ggcgcggaga acccgggcgg cgagtggtgg ctgccacccg gcgtcgcgcc cgatacggag
     3421 cgcggcgccg tgctgtacat tcacggcggc ggcttcatgc tggggtcgcc ggccagccac
     3481 cgcggcgtgg ccgcgagact ggcgcgccag accgggctgc cggtgctggc ggcgcattat
     3541 cggcttgcgc ccgagcacgc ctatccggac ggcctcgagg acctgcagct ggcctggcga
     3601 cgaatcaccc gcggcggcgc ccgcaaagtg gcgctggccg gcgattccgc gggcggatgg
     3661 ctggcgctgg cgctggcgca ttacgccgcg gccgccggcc tgccgcgccc gttcggcgcg
     3721 gcgctgttct cgccgctggt cgacctgtcc ggggcagcgc acattgcgcc cgcggcgcac
     3781 gacctgatgc tgccgcccgg attcgtcgcc gagagcattg cggcgtggcg cggcggcctg
     3841 ccggccgatg acccgcgctt cgacctgctc ggcgcgcagc tcgcggagct gccgcccctg
     3901 ttcatcagtc atgaccgcga tgagctgctt gccggcgatg cgcgccgcct ggcgcgggcc
     3961 gcacaagccg ccggcgtaca ggtgcgcgtc gaggaggggc gcggcctgtg gcacgcctgg
     4021 cccctgctgg ccggcctgct gcccgaggcc gacgcgaccc tgcgcagtgc ggcggccatg
     4081 ctgactccgg cggtcctgcc cggcggcccg ggatgatgcc gagcgccagc ccgacactcc
     4141 ggctgctgtg cggtgcggcg atgatcagct tcgcgccggt gttcgtgcgc ctgacgcccg
     4201 tcagccccac ggacagcgcc ttttaccgga cgctgttcgg cggcctcatg ctggccgcct
     4261 gggtgatcgc cgcccgcacg ccggtcgagc ggcgctccgg cctggcgctc ggcgcgctga
     4321 tcgccgcggg cgtgctgttc gccgcggatc ttgcggtctg gcaccgcagc atctggtacg
     4381 tgggcccggg ccttgccacc ctgctcggca atttccaggt tttcctgctg gctttcgtcg
     4441 gcatactttt ctttcgcgag aaggcgcgct gggaattgtt gctggcgatc cccatggccg
     4501 tcgtgggcct gggcatgatc gtcggcttcg agtggtcgac gctcgacgag cagtaccgct
     4561 ggggcatcgt gttcggcctg ctgaccgcgg tgttctatgc cggctacatc ctggcgctgc
     4621 gccgtgcgcg cgcggcgagc ccggccggat cgatcgtgtc ggacctgatg atcgtgtcgc
     4681 tggtctccgc tttcttcctg ttcgccggcg ccggggccgc aggcgcgccg gtcgaactgc
     4741 ccgacgcgcg ctcggcctgg ctgctggcgg cctatgcgct ggtcgcgcag gtgctgggct
     4801 ggctgctgat ctccggcagc ctgccgcatg tgccggcatc gcgggtcgga ctgatcctgc
     4861 tgttgcagcc gaccctggcg ttctgctggg acgtgctcat tttcgaccgg gccttcgggg
     4921 cgcgcgaagc actcggagcg gtgctggcca tcacggccat cggactgggg tcgcggccgc
     4981 ggcgctgagg cgggctaggt ccacaggcgg agcacgcccg ccgcaagcac gatctcgagg
     5041 cgcaccaggt cggccccgag caactcgccg tcggcctgcg ccggcagcgg ccggtcgcac
     5101 actatttcga gccgggtgct gcgggcgaag tgtgcgatgg gcagcgattc atgggtgccg
     5161 cggatcacgc gcggcgcgtg gcgcaggacc tgcagccggc tcagcgcgtc ggcccagacg
     5221 atgtccagcc ggccgtcatc gagctctgcg cgcggcgcca gccggaacag cccgcccgcg
     5281 aattgcccgt tgcacgcggc caccagcgtc acggcccgcc ggtcctctcc gccgtcccat
     5341 cgcagggtgc agacgggccg gtcgacgccg gcccacagcg cctccaccag ccccgccccg
     5401 tagccgagtg gccctggcat ccacttgtgt cgcagcgtcg cctgcgcaat cgcggcatcg
     5461 aagccgaaac cgacgccgtt gatgaaccac cgcccgttga cccgccccgc atcgatgctg
     5521 cgcggcgtgc acccgtcggc gagtcgccgg caggcggcgc gccagctgcg cggcagcccg
     5581 gccgacttga cgaagtcgtt gcccgtgccg agcggcacga tgccgagcgc cgtgacgtgc
     5641 ccgggcacgc tcagcaggcc gcgcgccact tcggagagcg tgccgtcgcc gccgatggcc
     5701 accagtcgca cgcgcccgcg cgcggcctcc gtcgcggccg cctcggccag gtggccggcg
     5761 ccgcggctga cgatcgtctc caccggcacg ccgtgacgct ccagcagcgc cacggcccgc
     5821 gcgccctggc ggcgcctgcg gccgctgccg gcggccgggt tggcgagcac gatcacggac
     5881 tccggcatcg ggcatcctcc cgggcgacgg gccgcagcat acctcagccc gaaccgcgcc
     5941 cggcgccgca cggcgcccgt gttccgggct agaatcgcgt ctcccggcct gcctgttcca
     6001 ccatgcctga aatcctgctc aagctagttc tcggctacct cgccggctcc gtggtcggca
     6061 gcctggtggt cggccgcgtg cgcggcatcg acatccgcaa cctcggcagc gggaacgccg
     6121 gcggcaccaa tgcgttgcgc acccagggca agggcttcgc gctcgccgtg gtgctcatcg
     6181 acgtgctcaa ggtggtgctg ccggtcgtgt tcctgaccgg cgctgcgctg ccgggcgtcg
     6241 gcgtggatgc cgcgatcgcg cgcgagaccg tgcaggtggc catcgcgacc ggcgccgtgc
     6301 tcggccatgt ctggccgctg tatttcggct ttcgcggcgg caagggcatg gccacgctgc
     6361 tgggcgccta cggcgccatc cagcccgcgc tgctcggcgt ggtggtgctg gcctgggcgc
     6421 tgctgttcgt gctgcgcggt tacgtcgggc tggcaaccct gttcgccgcg gcgatggcgc
     6481 cggtctggct ggcggcgacc ggccagtcgg ggcggccgct gttctggttc gcgctcctga
     6541 tggcaggctt cgtcgcatgg acgcaccgga gcaacatcgc gcgcatgcgg gacggcaaag
     6601 agacccgcat gtttcccggc ctgctgggcc ggcgccggtg aattacagcg tcgccttgct
     6661 gcgggcgctc gcgccgggca caccggtgtc gggccaggcg ctcgggcgcg agctcggcgt
     6721 cagccgcgcg gcggtctgga aggccacccg gcgcctaccg gctcgagcag ccgctggtgc
     6781 tgctcgacgg tgacgccatc cggcaaggcc ttcctgccgg cgtggcgcgt cgcgtcgggt
     6841 tgctggaggt gctgccggaa accgcctcca ccaatgcgcg cgtgctggcg gccgaacgcc
     6901 cggtcggcga gctgggggtg tgtcttgccg aataccagtc ggccggccgc ggccggcgg
//
LOCUS       DAOMPG010000118         6900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4706_length_6900_cov_3.916289, whole genome shotgun sequence.
ACCESSION   DAOMPG010000118 DAOMPG010000000
VERSION     DAOMPG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4706_length_6900_cov_3.916289"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /locus_tag="PLS34_07610"
     CDS             <1..1937
                     /locus_tag="PLS34_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87371.1"
                     /translation="RERIRRGLRQAAELWRADDGDAEVFAEFARTHFAGDQATLDAMF
                     DRYQYLLERLGGHMNKLVQEFRRHSDLDHGPILPFDMLFAGYNPAAHVSEDMFRNRLA
                     FVALLNFPLTTLEERLAEGGQWSRRQWAEARLAQRFGRRVPAEVNQEISRAGARADQY
                     IAEYNIWMHHLVNDQGERQFPAGLRLLSHWNLRDEIKASYGLEDQAAALARQRTIQRV
                     MEHIVEQTIPAVVIDNPQVDWNPHSNQVAPAAVVDGGEPPAAGFRPDNAPEPDERYRV
                     LLDTYRAARLADPYSPTAPTLIARRFDEDREIPEARVEAMLKEVLASPLVPRVAKLIE
                     ARLGRPLEPFDIWFNGFRPSSRYTEAELDAIVAERYPTAAAFEADLPDLLTKLGYSPE
                     RAAYLAANIVVEPARGSGHAWGAGMRSEKAHLRTRVEAGGMNYKGYNIAVHELGHNVE
                     QTFSLNDIDHTLLEGVPNTAFTEAIAFVFQARDRELLGLAQPDASAEALQTLNSFWGT
                     FEIGGVALVDMAVWHWMYDHPEATPAELKAATVRIAKDIWNEYYAPVFGVRDVVLLGI
                     YSHMIHSFLYLPDYPIGHLIAHQIEEQVRKTGDVAAEVERMSRIGSIAPDLWMQEATG
                     SPVGAGAMLAATERALEQLE"
     gene            1934..5158
                     /gene="putA"
                     /locus_tag="PLS34_07615"
     CDS             1934..5158
                     /gene="putA"
                     /locus_tag="PLS34_07615"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HRP87372.1"
                     /translation="MSGQGEQEVAATERTAGHPVPADGYFIFQAGQAPVSSVAERVNT
                     RYLADEATLVRELADLARAGADGAAQIRDTATRLVAAVRKNRKRVSGLDAFLQQYDLS
                     SQEGVVLMCLAEALLRVPDKATADRLISDKLSAADWKSHLGTSDSTFVNASTWGLMLT
                     GRIIRPASQDVEQPARFMQRLVSRLGEPVVRGAMRQAMRIMGHQFVMGRTIGDALKRA
                     SSGEGAAYRYSFDMLGEAALTAADSQRYLEAYHAGIRAIAAAERRPDSIFSADSISVK
                     LSALFPRYEFAKRERVLEELTPRIIELAVAARKAGVGLTIDAEEADRLQISLELFERV
                     FRDEALAAWDGFGLAVQSYLKRGLDACRWIIALAEETGRCIPVRLVKGAYWDAEVKRA
                     QENGLETYPVFTRKVNSDVSFLACARALLAARGRVYPQFATHNAHSVASIIHFAGDNR
                     QFEFQRLHGMGEELYAEVIGERQFNLPCRVYAPVGNHEDLLPYLVRRLLENGSNTSFV
                     NRIVDEDAPIEDIVADPVALVDGLEIVPHPRIPAPAALYGAERRNSRGLNLADPGELA
                     PLAEAMRAALARPLRAAPIVSGREEDGEAHPVHCPADRRRVLGEARWASPELARRALE
                     AAAKAQPRWNALGAAARAGMLERAADLFEEHTAELLALCVREGGRTLPDSLSELREAV
                     DFLRYYAAQARREFAAPLRLPGPTGESNDLSWHGRGVFLCISPWNFPLAIFAGQVAAA
                     LVTGNTVLAKPAEQTPLVAAVVVKLMHAAGVPGEVLHLLPGSGPVLGEALLSDERLAG
                     VAFTGSTEVARIINRALAARDSAIATLIAETGGQNAMLVDSSALPEQVVLDTVKSAFN
                     SAGQRCSALRVLFLQEDIADRTLRLLKGFMQELQVGDPGLLSTDVGPVIDAEALATLE
                     AHVSRMEQEGRILARCHLPAGASHGTFIAPTVVQLESIRQLEREVFGPVLHVVRYAAQ
                     DLDRIIGEINATGFALTLGVHSRIDARAREVFARAKAGNVYVNRNMVGAVVGVQPFGG
                     SGLSGTGPKAGGPGYLHRFMTERTLTINTSAVGGNASLLSLSEL"
     gene            5172..6530
                     /locus_tag="PLS34_07620"
     CDS             5172..6530
                     /locus_tag="PLS34_07620"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRP87373.1"
                     /translation="MAISIFDLIKIGVGPSSSHTMGPMLAARRFLLDAREAGWFERIE
                     RVGAQLYGSLALTGKGHCTDRAILLGFEGEVPATLEPDTVEARLERIRGAGRLRLLGE
                     REIGFDESMELLFHKEQVLPRHVNGMRFTALDAAGATLGSETFYSVGGGFIVRGDEPD
                     ESTGATVSVPFPFDNTAELLEQCRRHGMRIHELVLANEEAWRAAAETRSHLLELWQVM
                     KECVRHGFEAEGVLPGVLKVPRRAPRLYRELCGEGAAGDFMDWVNAYALAVNEENAAG
                     ARIVTAPTNGAAGIIPAVLHYYRRFVSNADEEGIVRFLLTAGAIAILYKKNASISGAE
                     MGCQGEVGVACSMAAGGLVAAMGGSNAQVENAAEIGMEHNLGLTCDPIAGLVQIPCIE
                     RNAMGAVKAVNAARMAFRGDGSHKVSLDQVIATMRQTGLDMSTSYKETSRGGLAVNVP
                     EC"
BASE COUNT         1095 a         2331 c         2421 g         1053 t
ORIGIN      
        1 aacgcgagcg catccggcgc ggattgcgcc aggcggcgga gctctggcgc gccgacgacg
       61 gcgacgccga ggtgtttgcc gagttcgccc gtacgcattt cgccggcgac caggcaacgc
      121 tggacgccat gtttgaccgt taccagtacc tgctggaacg cctcggcggc cacatgaaca
      181 agctggtgca ggagttccgg cgccactccg acctggacca cggtccgatc cttcccttcg
      241 acatgctgtt tgccggctac aacccggcgg cgcacgtgag cgaggacatg ttccggaacc
      301 ggctggcctt cgtcgcgttg ctgaatttcc cgctcaccac cctcgaggaa cggcttgccg
      361 agggcgggca gtggtcgcgc cgccagtggg cggaagcacg cctcgcacag cgcttcggcc
      421 ggcgggttcc ggcggaggtc aaccaggaaa tttcacgcgc cggcgcccgc gccgaccagt
      481 acatcgccga gtacaacatc tggatgcatc acctggtcaa cgaccagggc gagcgccagt
      541 ttccggccgg cctgcggctg ttgtcgcact ggaacctgcg cgacgagatc aaggccagct
      601 acgggctcga ggaccaggcg gccgcgctgg cgcgccagcg caccatccag cgcgtgatgg
      661 agcacatcgt cgaacagacc atcccggcgg tggtgatcga caatccccag gtggactgga
      721 acccgcacag taaccaggtg gcgccggctg ccgtggtgga cggcggcgaa ccgcccgcgg
      781 ccggtttccg gcccgacaat gcacccgagc cggacgagcg ctaccgggtg ttgctggaca
      841 cctaccgtgc agcgcgtctc gccgatccgt attcccccac cgcgccgacg ctgatcgcgc
      901 ggcgcttcga cgaggatcgc gagattccgg aggcccgggt cgaggccatg ctcaaggagg
      961 tgctcgcgtc gccgctggtg ccgcgcgtgg cgaagctcat cgaggccagg ctcggccggc
     1021 cgctcgagcc cttcgatatc tggttcaacg gcttccgccc gtcgagccgg tatacggagg
     1081 cggaactcga tgccatcgtc gccgagcgct acccgaccgc cgcagcgttc gaggccgacc
     1141 tgcccgacct cctcacgaag ctcggctaca gcccggagcg ggcggcatac ctcgcggcca
     1201 atatcgtcgt cgagccggcg cgcggctccg gccacgcctg gggggcgggc atgcgctccg
     1261 agaaagccca cctgcgcacg cgggtcgagg ccggcggcat gaactacaag ggctacaaca
     1321 tcgccgtgca cgagctcggc cacaacgtcg agcagacttt ctcgctgaac gacatcgacc
     1381 acaccctgct cgaaggcgtg ccgaatacgg cgttcacaga ggccatcgcc ttcgtgttcc
     1441 aggcgcggga ccgcgagctg ctcgggctgg cgcagcccga tgcgtcggcc gaggccttgc
     1501 agaccctgaa cagtttctgg ggaaccttcg agatcggcgg cgtcgcgctg gtcgacatgg
     1561 cggtgtggca ctggatgtac gaccacccgg aggcgacgcc ggcagagctc aaggcggcga
     1621 cggtgcggat tgcgaaagac atctggaacg agtactacgc gccggtgttc ggggtgcgcg
     1681 acgtcgtgct gctgggcatc tactcgcaca tgatccattc cttcctgtac ctgcccgact
     1741 acccgatcgg ccatttgatc gcgcaccaga tcgaggagca ggtccgcaag acgggcgacg
     1801 tcgcggccga ggtcgaacgg atgtcgcgaa tcggcagcat tgcgcccgac ttgtggatgc
     1861 aggaggcgac cggctcgccc gtcggtgccg gcgcgatgct cgcggcgacc gagcgtgccc
     1921 tggaacaact cgaatgagcg gccaaggaga acaggaagtg gcagcgacgg agcgcacggc
     1981 cggtcacccg gtcccggcgg acgggtactt catattccag gctggacagg cgcccgtgtc
     2041 gagcgtcgcg gaacgcgtca atacgcgcta cctggccgac gaggcgacgc tggttcgcga
     2101 actggcggac ctcgcgcgcg cgggtgccga cggcgcggcg cagattcgcg acacggccac
     2161 gcgcctggtg gcggccgtgc ggaagaaccg caagcgggtc agcggcctgg acgccttcct
     2221 gcagcagtac gacctgtcca gccaggaggg cgtggtgctg atgtgccttg cagaagcgct
     2281 gctgcgcgtg cccgacaagg cgacggctga ccggctgatc tccgacaagc tgtccgcggc
     2341 cgactggaag agccatctcg gcaccagcga ctccactttc gtgaacgcct cgacctgggg
     2401 gttaatgctc accggccgca tcatccggcc cgccagccag gacgtcgagc agccggccag
     2461 gttcatgcag cgcctggtga gccggctcgg cgagccggtg gtgcgcggcg cgatgcgcca
     2521 ggcgatgcgc atcatgggcc accagttcgt catggggcgc accatcggcg atgcgttgaa
     2581 gcgcgcttcg tcgggcgagg gcgccgcgta ccgctactcc ttcgacatgc tcggcgaggc
     2641 cgcgctcact gccgccgaca gccagcgcta cctcgaggcc taccatgccg gcatccgcgc
     2701 cattgccgcc gcggaacgcc gaccggactc gatcttctcg gccgacagca tctcggtgaa
     2761 actgtcggcc ctgtttccgc gctacgagtt cgccaagcga gagcgggtgc tcgaggaact
     2821 gacgccgcgc atcatcgagc tggcggtcgc cgctcgcaag gccggtgtcg ggctgaccat
     2881 cgacgccgaa gaggccgatc ggctgcagat ctcgctggaa ctgttcgagc gcgtgttccg
     2941 cgacgaggcg ctggcggcgt gggacggttt cggcctggcg gtacagtcct atctgaagcg
     3001 cggcctcgac gcctgtcgct ggatcatcgc gctggcggag gaaaccggcc gttgcattcc
     3061 cgtccggctg gtcaagggcg cgtattggga tgcggaggtc aaacgggcgc aggagaacgg
     3121 gctggagacc tacccggtct tcacgcgcaa ggtcaacagc gacgtatcgt tcctggcctg
     3181 tgcacgcgcg ctgctggccg cgcgcggccg ggtctatccg cagttcgcta cgcacaatgc
     3241 gcacagcgtt gccagcatca ttcatttcgc cggggacaac cgccagttcg agttccagcg
     3301 cctgcacggc atgggcgaag agctgtacgc cgaggtcatc ggggaacggc agttcaacct
     3361 gccctgccgc gtttacgcac cggtcggcaa ccacgaggac ctgttgcctt acctggtgcg
     3421 ccgcctgctg gagaacgggt ccaacacctc gttcgtgaac cgtatcgtcg acgaggatgc
     3481 gcccatcgaa gacatcgtcg ccgacccggt ggcgcttgtg gacggcctgg aaatcgtccc
     3541 gcatccgcgc atccctgcgc cggcggccct gtacggcgcc gagcggcgga actcgcgcgg
     3601 cctcaacctg gcggaccccg gtgaactggc gccgctggcc gaggcgatgc gggccgcgct
     3661 cgcccgtccg ctgcgcgcgg cgccgatcgt gagcggccgc gaggaagacg gtgaggccca
     3721 cccggtccat tgcccggccg atcggcgcag ggtgctcggc gaggcccgct gggcctcccc
     3781 cgaactcgcc cggcgggcgc tcgaagcggc tgcaaaggcg cagccgcgct ggaatgcgct
     3841 cggtgccgcc gcccgggcgg gcatgctgga gcgggccgcg gacctgttcg aggagcatac
     3901 ggcggagctg ctggccttgt gcgtccgcga gggcggcagg acgctgcctg actcgctgtc
     3961 cgagctgcgg gaggcggtgg acttcctgcg ctactacgcc gcgcaggcgc gccgtgagtt
     4021 cgccgcgccg ttgcgcctgc ccggaccgac cggcgagagc aacgacctgt cctggcatgg
     4081 ccgcggggtg ttcctgtgca tcagcccgtg gaactttccc ctggcgattt ttgccggcca
     4141 ggtggcggct gcgctggtga ccggcaatac cgtactggcc aagcctgccg agcagactcc
     4201 gctggtggcc gcagtcgtcg taaagctcat gcacgcagcc ggcgtgccgg gcgaggtgct
     4261 gcacctgttg ccgggcagcg gcccggtgct gggcgaggcg ctgctgtccg acgagcgcct
     4321 ggcgggcgtg gcgttcacgg ggtcgaccga ggtcgcccgg atcatcaatc gtgcgctggc
     4381 cgcgcgcgac agcgcgatcg ccacgctgat cgccgaaacg ggcgggcaga atgccatgct
     4441 ggtagacagc tcggcgctgc ccgagcaggt cgtgctcgac accgtcaagt ccgcgttcaa
     4501 cagcgccggc cagcggtgct ccgcattgcg ggtgctgttc ctgcaggagg acatcgcgga
     4561 ccggacgctg cgcctgctca agggcttcat gcaggaattg caggtggggg accccggcct
     4621 gttgtcgacc gacgtcggcc cggtgatcga cgccgaggcg ctggcgacgc tggaagcgca
     4681 cgtgagccgc atggaacagg aaggacgcat cctggcgcgc tgtcatctgc ccgccggagc
     4741 cagtcacggc accttcatcg ccccgaccgt ggtgcaactg gaaagcatcc gccagcttga
     4801 gcgcgaagtc ttcgggcccg tgcttcacgt ggtgcgttac gcggcgcagg acctcgaccg
     4861 gatcatcggc gagatcaacg ccaccggctt cgcgctcacg ctcggtgtgc acagccggat
     4921 cgacgcgcgc gcccgcgagg tctttgccag ggcgaaagcc ggcaacgtct acgtcaaccg
     4981 gaacatggtc ggtgcggtgg tgggagtgca gcccttcggc ggctccggcc tgtcgggcac
     5041 cggcccgaaa gccggcgggc cgggctacct gcatcgcttc atgaccgagc gcacgctcac
     5101 catcaacacc tcggcagtgg gcggcaacgc cagcctcttg tcgctgtctg agctttgagc
     5161 ggcgtcccgt catggccatc agcattttcg acctgatcaa gatcggcgtg ggaccatcga
     5221 gctcccacac catgggcccg atgctggcgg caaggcgctt cctgctggac gcacgcgagg
     5281 caggatggtt cgagcgcatc gagcgggtcg gcgcgcagct ttacggttcg ctggccctca
     5341 ccggcaaggg gcactgcacc gaccgcgcca tcctgctggg cttcgagggc gaagtgccgg
     5401 caaccctgga gccggacact gtcgaggcgc ggctcgaacg gatccggggg gcgggccgcc
     5461 tgcggctgct cggcgagcgg gagatcggct tcgacgagtc gatggaattg ctgttccaca
     5521 aggagcaggt cctgccgcgg cacgtcaacg gcatgcgctt cacggcgctc gacgcggcgg
     5581 gcgcgacgct cggcagcgag actttctatt ccgtcggcgg cggcttcatc gtgcgtggcg
     5641 acgagccgga cgaatctacc ggggcaaccg tcagcgtccc gttcccattc gacaacaccg
     5701 ccgaactgct cgagcagtgc cgccgtcacg gcatgcgcat ccacgaactc gtgctggcca
     5761 acgaggaggc atggcgcgcg gcggcagaga cgcgctcgca cttgctcgaa ctctggcagg
     5821 tgatgaagga atgcgtgcgc cacggctttg aggccgaagg cgtgctgccg ggcgtgctga
     5881 aggtaccgcg ccgggcgccg cgactttatc gcgagctctg cggcgagggt gcggccggcg
     5941 acttcatgga ctgggtcaat gcctacgcgc tggcggtcaa cgaggaaaac gcggccggcg
     6001 cgcgcatcgt cacggcgccc accaacggcg cggcggggat cattcccgcg gtgctgcatt
     6061 actacaggcg cttcgtgagt aatgcggacg aggagggcat cgtccgcttc ctgttgaccg
     6121 ccggcgcaat tgcaatcctc tacaagaaga acgcttcgat ctccggcgcc gagatggggt
     6181 gccagggcga ggtcggcgtg gcctgctcga tggcggcggg cggcctggtg gcggcgatgg
     6241 gcggcagcaa cgcccaggtc gagaacgccg cggaaatcgg catggagcac aacctgggac
     6301 tgacctgtga cccgatcgcc ggcctggtgc agattccctg catcgagcgc aatgcaatgg
     6361 gagccgtgaa ggccgtgaac gcggcacgca tggcgttccg cggcgacggc agtcacaagg
     6421 tgagcctgga ccaggtcatc gccaccatgc gccagaccgg gctggacatg tcgaccagct
     6481 acaaggaaac ctcgcgcggc ggcctcgcgg tcaacgtccc cgagtgctga ccgcccgaaa
     6541 ccggggtcag acaccaattt ccggtcgttc gcgggaccgc gccgccatgg gcgcccttcg
     6601 ctcggcgaat tccgagctcg ctcagtgcgc ccatgacgtc ccggtcccgc tgccgcagcg
     6661 atttcggcag cgcctcgcct tcgcggtgcc gctgacgatc gccacgccgg cgaccggcgt
     6721 tgcgggggcg gactgaccca gcgtggtcgc tggcgatcgg ggcatagcgc cgactcagca
     6781 gccggccgcg gccgcaggcg gtgtcgccgg gccgggcaac cgtcgccgcc accctcgtaa
     6841 tcccggcagc gggtgggggg tgtcataggc cggccgagcg agtttttcct tgtccgagcg
//
LOCUS       DAOMPG010000119         6875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4728_length_6875_cov_4.864076, whole genome shotgun sequence.
ACCESSION   DAOMPG010000119 DAOMPG010000000
VERSION     DAOMPG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_6875_cov_4.864076"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLS34_07625"
     CDS             complement(<1..830)
                     /locus_tag="PLS34_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRP87374.1"
                     /translation="MLHVLLIVLGIGLLTAGGEALIRGALAAARRLGVSPLLSGLVIV
                     GFGTSMPELVVSLDAAVNQRPDIAVGNVVGSNIGNILLILGLCAAITPLAVHPMALRR
                     DAVVMVGASLLFLLLAHGGLLGRFEGLAWLAGLLAYLGWAYRTERAQVTPGGELLVAE
                     SEEITALPGSTRLMAAAVLGGLGLLIAGSRVLIIGAVGLAEEFGVSEAVIGLTLVAVG
                     TSLPELTVGVMAALRRHADVAVGNILGSNIFNLLGILGVAALVEPLAVNPRILAFDQW
                     V"
     gene            1099..1446
                     /locus_tag="PLS34_07630"
     CDS             1099..1446
                     /locus_tag="PLS34_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87375.1"
                     /translation="MATSPPDDSLPNLRQLGLFALAAAVQVSIIAIGRLWLRFSSPEE
                     AGLPPDERDEAIERRAIVFAYYVLIGGVIIAAIIMPFYVGGWEIVNAAILAIVVAELV
                     QDGLTITGYRRLS"
     gene            1443..1661
                     /locus_tag="PLS34_07635"
     CDS             1443..1661
                     /locus_tag="PLS34_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP87376.1"
                     /translation="MIQGVITNNIRTLRFLHGEMTQAELGRLIGVTRQTIAAIEAGKY
                     SPSLEAAFRIAAVFEVPLGEVFSWEPLE"
     gene            1687..2397
                     /locus_tag="PLS34_07640"
     CDS             1687..2397
                     /locus_tag="PLS34_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRP87377.1"
                     /translation="MKRLPSWIPVFLRALPKMLKAARPRTVRRCPICAYTGYFAPVGQ
                     PPRLEAKCPRCGSKERHRLFWLWLAGRPDKLAEPVLHFAPERTLSERFRAMYREYQTA
                     DLYKDADLKLDIEDIDLPDGSQNTIICNHVLEHVDDRKALGELARVLAPTGRLICSVP
                     IIEGWERTYENDAVTTAQDRTLHYGQKGHVRYYGRDLRDRLRAAGFTSIEEVTAEGPA
                     VVEFGLWRGEKFFVCSKT"
     gene            complement(2408..2908)
                     /locus_tag="PLS34_07645"
     CDS             complement(2408..2908)
                     /locus_tag="PLS34_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRP87378.1"
                     /translation="MSRAFRAAALAACLLSAAIVAADEVVLRVSGAVDTPLELTRADL
                     DAMPRDEFRIPTQHDRSVMETWAGVPMIEILRKAGAPVGERLRGPNMAGYVVVIAEDG
                     FRAVYALAEIEPRFSPERRILVADRLDGEPLGDKFGNLRIANQGEGAFSRWVRKVVAL
                     EVRLAD"
     gene            3090..4169
                     /locus_tag="PLS34_07650"
     CDS             3090..4169
                     /locus_tag="PLS34_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRP87379.1"
                     /translation="MRNATITGWGKCMPPAVLSNDDISTFLETDDEWITSRTGIRERR
                     ISHVPLEEMGAVAAQRALACAGMEAGELELIVFGTTSFDQQVPNTASGVQKRIGAVNA
                     GAMDVNTACTSYLYGLTTAAALISQGVVNNAVVIGGELISPLMDWTDRGVAVLFGDGC
                     AATVLQATDADVGLRASKLGCYGEVREILLVHGMGQRYSNCGWVNGDTQWVFEGQEIF
                     KRAVHGMSQACEHVLTSCGMTADDVDLVVPHQANLRIIEAVAKRAHVPMERCFVNVHR
                     YGNMSAATVPVALVEALEEGRVEPGAELLLTGFGGGLTWSAHVLRWGERVTPLGESDV
                     QLPPCEKSALAMVRGYLAAKRGSHF"
     gene            4395..5024
                     /locus_tag="PLS34_07655"
     CDS             4395..5024
                     /locus_tag="PLS34_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HRP87380.1"
                     /translation="MNASRGLRALSGLLVVTALASLSGCAFGTRHAELNYPPSKGGAD
                     VIATAEAAGAPAAAGIDVILAVTDMRASKERIGNVRNGFGMDTANVVTEADIRAWVED
                     AFTRELTDAGYVVVPAGSGTGPETAITLGAEVTKVYCDAYLTYDGEVLMTVTLARQGA
                     PAQVKAYEGSGSVGLSWAATAKSYGESLSLALQDGIRQVMADLAAYRSP"
     gene            complement(5038..6624)
                     /locus_tag="PLS34_07660"
     CDS             complement(5038..6624)
                     /locus_tag="PLS34_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRP87381.1"
                     /translation="MPLQALLRVPTLQLLAVAALSAALAPPALAGDRPDPRPRHEFTT
                     DEAFDAAAVPAYAGRHEAVYAQIDRDLEAHVDALQRWVRQRSISAVDDGIREMAELVA
                     GDLRDLGFGEVAIVETDGHPGVFGYFDAGAEKTLLVYMMYDVQPIEPNWRIADPFAGE
                     LVETELGTVLMARGATNQKGPQRAFLNAVEAILKAEGTLPVNLAVVAEGEEELGSTHF
                     DQVLAPYAERLRQADGAFFPMNLQTPDGSVGLNLGVKGIVYFELEARGGEWGGPQKAE
                     IHGSLKSVVDAPALRLVNAIASMVSPDGNTILIDGYYDGLRGPTEDEQRLINGLAQSG
                     QFDRMREQLGVTRWIDGLEGRDIIVRYLFDPTLNINGMWSGYTGPGGKTILPHKATAK
                     IDTRLPPGLEPETAWTKFREHLDAHGFTDVEIVQIEGRGYPAAASPVDSPIVQAAIGV
                     FNKYGHTPAVSPWLGGSAPFYQFTRNLGLPFVFGGLGHGAGAHAPDEYMLIHPADGVG
                     AAGLAEVEKSYVDLLYALAE"
BASE COUNT         1150 a         2334 c         2326 g         1065 t
ORIGIN      
        1 acccactggt cgaaggcgag aatccgcgga ttgaccgcca gtggctcgac cagcgccgcg
       61 acgccgagaa tccccagcag gttgaaaatg ttgctgccca ggatgttgcc gacggccacg
      121 tccgcgtgac gacgcaatgc cgccatcacg ccgacggtca actccggcaa ggaggtaccc
      181 accgcgacga gcgtgagccc gatgacggcc tcggacacac cgaattcttc cgccaggccg
      241 accgccccga tgatcagcac gcgtgagccc gcgatcagca gccccaggcc gccgagcacc
      301 gcggcggcca tcagccgcgt cgagccgggc aaggcggtaa tctcctcgga ctccgcgacc
      361 agcaattcgc cgccgggggt gacctgcgcg cgctccgtac ggtaggccca gccgaggtag
      421 gccaacaggc cggccagcca ggccagcccc tcgaagcggc ccagcaggcc gccgtgggca
      481 agcagcagga acagcaggct cgcgcccacc atgaccaccg catcgcgccg tagcgccatc
      541 gggtgcacgg ccagcggcgt gatggccgca caaagcccga gaatcagcag gatattgccg
      601 atgttgctgc cgacgacatt gccgacggcg atgtcgggcc gctggttgac ggcggcatcg
      661 agcgacacca ccagctccgg catcgaggtg ccgaagccga cgatcaccag tccgctcagc
      721 agcggcgaga cgcccagccg acgggccgcc gccagcgcgc cgcgaatcaa ggcctcgccg
      781 ccggcggtca gcagtccgat accaagaaca atcaacagga catgcagcat cgtgcggact
      841 caactgaacg gaccgggatc agcgggggca agcgagccct cggcccgcct ggcggtggat
      901 tctaccgccc cctgggcgat ccctgccgaa agttaaagtt actagatatc ttgacaagaa
      961 tatttcacat catgtaaaac ttatcagtca cacaaccccg cgcccacgtt tagggacctc
     1021 aagatgcctc accgggaaaa agctgcctgg ctctcgctca tcgcaatcgt ctgtgcgttc
     1081 gggccgtatt ttgcgctcgt ggcgacatct ccgcccgacg attcactgcc gaacctgcgc
     1141 cagctcgggc tgtttgcgct ggccgccgcg gtgcaggtct cgatcatcgc gatcgggcgc
     1201 ctctggctgc gattcagttc gcccgaggaa gccggcctgc ccccggacga gcgcgacgag
     1261 gccatcgagc gtcgcgcgat cgtgttcgcc tactacgtgc tgatcggcgg ggtcattatt
     1321 gcggccatca tcatgccctt ctatgtcggc ggctgggaga tcgtcaacgc ggcgatcctg
     1381 gcgatcgtcg tcgcggaact ggtgcaagat ggcctcacca tcaccggcta ccgccgcctg
     1441 tcatgatcca gggcgtcatc accaacaaca tccgcacact gcgctttctt cacggggaga
     1501 tgacccaggc cgagctcggc aggctgatcg gggtgacccg gcaaaccatc gccgcaatcg
     1561 aagcgggcaa gtactcgcca tccctggaag ccgccttccg catcgccgcg gtgttcgaag
     1621 tgccgctggg cgaggtgttc tcatgggagc cattggaatg aacgtgatcc gaaggtcagc
     1681 acgcgcatga aacgccttcc atcgtggatt cccgtgttcc tgcgggcgct gccgaagatg
     1741 ctcaaggcgg ccaggccgcg cactgtccgc cgctgcccga tctgcgccta cactggctac
     1801 ttcgccccgg tcgggcagcc gccgcgactc gaggccaagt gcccccgctg cgggtcgaag
     1861 gagcgccacc ggctgttctg gctctggctg gcgggccgtc cggacaagct ggcggagccg
     1921 gtcctgcact tcgcgccgga gcggacgttg tcggagcgct tccgggcgat gtaccgggag
     1981 taccagacgg cggacctcta caaggacgcc gacctcaagt tggacatcga ggacatcgac
     2041 ctgccggacg gcagccagaa caccatcatc tgcaatcacg tcctggaaca cgttgatgac
     2101 cgcaaggcct tgggcgagct ggcccgcgtg ctggcgccga ccggccggct gatctgctcc
     2161 gtgccgatca tcgagggctg ggagcggact tacgagaacg acgccgtgac gacggcgcag
     2221 gatcgcacgc ttcattacgg gcagaaaggc catgtccgtt attacggtcg cgacttacgc
     2281 gaccggctgc gggcggcggg cttcacctcc atcgaggagg tcaccgctga gggcccggcg
     2341 gtggttgagt tcgggctgtg gcgcggcgag aagttcttcg tctgcagcaa gacttagccg
     2401 gcgccgacta atcggcgagc cggacttcca gcgccacgac cttgcgcacc caccgcgaga
     2461 acgcgccttc cccctggttg gcgatgcgca ggttgccaaa cttatcgccc agcggctcgc
     2521 catccaggcg gtcggccacg aggatgcggc gttccggcga gaagcgcggc tcgatctcgg
     2581 ccagcgcata gaccgcgcgg aagccgtcct cggcaatcac cacgacgtag ccggccatgt
     2641 tggggccgcg cagccgctcg cccaccggcg cgccggcctt gcgcaggatc tcgatcatcg
     2701 gcacgccggc ccaggtctcc atcacgctgc ggtcgtgctg ggtgggaatc cggaattcgt
     2761 cgcgtggcat ggcgtccagg tcggcgcgcg tcagctcgag cggcgtatcc accgcgccgc
     2821 tgacccgcaa cacgacctcg tcggccgcga cgatcgcggc gctcagcagg cacgcggcga
     2881 gggccgcggc acggaaggct ctgctcaaca tcggcgacaa catgttcaac ctctgccagg
     2941 agtggcgcgg tgatgtggag aaaccgcttc ggcgcacagt atgttgcctg cagcgacggc
     3001 ggtccagcgc cccgcactgg tgcgccgcga atggcatccg gcaggtgttt ccgggctaga
     3061 atcacgcaat ttgctgcggg gatgcctggg tgagaaacgc caccatcacc ggctggggca
     3121 agtgcatgcc gccggccgtg ctgtcgaacg acgacatctc gaccttcctc gagaccgacg
     3181 acgagtggat caccagccgc acgggcatcc gcgagcggcg tatctcgcac gtgccgctgg
     3241 aggagatggg cgcggtcgcg gcgcaacgcg cgctcgcttg cgccgggatg gaggccggcg
     3301 agctcgagct gatcgtgttc ggcactacca gcttcgacca gcaggtaccc aatacggctt
     3361 ccggcgtgca gaaacgcatc ggcgcggtaa acgcgggcgc gatggacgtg aacacggcct
     3421 gcaccagcta tctctacggc ctcaccacgg ctgccgcgct gatctcgcag ggcgttgtga
     3481 acaacgccgt ggtgatcggc ggcgagctga tttcgccgct catggactgg accgaccgcg
     3541 gcgtcgcggt gctgttcggc gacggctgcg cggccaccgt gctgcaggcc accgacgcgg
     3601 acgtgggcct ccgcgccagc aagctcggct gctacggcga ggtgcgcgag atcctgctgg
     3661 tgcacggcat gggccagcgc tattccaact gtggctgggt caacggcgac acccagtggg
     3721 tgttcgaggg ccaggagatc ttcaagcgcg ccgtgcacgg catgagccag gcgtgcgagc
     3781 acgtgctcac cagctgtggt atgaccgcgg acgacgtcga cctggtggtg ccgcaccagg
     3841 ccaacctgcg catcatcgag gcggtcgcca agcgggcgca cgtcccgatg gagcgctgct
     3901 tcgtcaacgt gcaccgctac ggcaacatgt cggcggcaac ggtgccggtg gcgctggtcg
     3961 aggcgctgga ggaaggccga gtcgagccgg gtgcggagtt gctgctgacc ggcttcggtg
     4021 gcgggctgac ctggtcggcg catgttctgc gctggggcga gcgtgtcacg ccgctgggcg
     4081 aatccgacgt ccagttgccg ccctgcgaga agagcgcgct ggcaatggtg cggggctacc
     4141 tggcggcgaa gcgcggcagc catttttgag cgtgcggcgc ggccctctgg agtcctgccg
     4201 cgcggcgcgc cacgcaggat ctacaaggac gccttgcagt aggcattccg cgggtgcaag
     4261 ccggtttcat cccctcgcaa actgccggaa tcgggccata cttgagatgc caaacaggtc
     4321 tgtttgtctc tcgcggcaac gagcggaaag caccgcccga agacctggaa cgccgcggga
     4381 gcgaggtcag ggaaatgaac gcctcacgtg gtctcagggc attgagcggc ctgctggtcg
     4441 tcacggccct ggcgagcctg tccggctgcg ccttcggcac ccggcacgcc gaactgaatt
     4501 acccgccctc aaagggcggc gctgacgtca tcgcgaccgc cgaggccgcc ggcgccccgg
     4561 cagcggcggg cattgatgtc atcctggcgg tcaccgacat gcgcgccagc aaggagcgca
     4621 tcggcaatgt ccgcaacggc ttcggcatgg acaccgccaa cgtcgtcacc gaggccgaca
     4681 ttcgtgcctg ggtggaggat gccttcactc gggagctgac ggacgcaggc tacgtggtgg
     4741 tcccggcggg aagcggcacg ggcccggaaa cggccattac gcttggtgcg gaagtcacca
     4801 aggtgtattg cgacgcctac ctgacctatg acggcgaggt gctgatgacg gtgaccctgg
     4861 cgcgccaggg cgcaccggct caggtaaagg cctacgaagg ctcgggcagc gtcggactca
     4921 gctgggcggc caccgcgaag agctacgggg agtcgctgtc gctcgccctg caggacggca
     4981 tccggcaggt gatggcggac ctggcggcct accgctcacc gtagcggtag ccgggcctca
     5041 ctcggccagc gcgtacagca ggtccacgta ggacttctcg acttcggcga gtcccgcggc
     5101 gccgacgcca tccgccgggt ggatgagcat gtactcgtcc ggcgcgtgcg cgccggcgcc
     5161 atggccgagc ccgccgaata cgaagggcag gccgaggttg cgcgtgaact ggtagaacgg
     5221 ggcgctgccg cccagccagg gcgagacggc gggcgtgtgg ccgtacttgt tgaacacgcc
     5281 gatggcggcc tgcacgatcg ggctgtccac cgggctggcg gccgccgggt agccgcgacc
     5341 ctcgatctgc acgatctcga cgtccgtgaa gccgtgcgca tcgaggtgct cgcggaactt
     5401 cgtccaggcg gtctccggct cgaggcccgg cggcagccgg gtgtcgatct tggcggtggc
     5461 cttgtgcggc aggatggtct tgccgccggg cccggtgtag cccgaccaca tgccgttgat
     5521 gttgagcgtc gggtcgaaca ggtagcgcac gatgatgtcg cggccctcca ggccgtcgat
     5581 ccagcgcgtc acgccgagct gctcgcgcat gcggtcgaac tggccgctct gcgccaggcc
     5641 gttgatcagc cgctgctcgt cctcggtggg gccgcgcagc ccgtcgtagt agccgtcgat
     5701 gagaatggtg ttgccgtcgg ggctcaccat ggaagcgatg gcgttcacca gccgcagcgc
     5761 cggcgcatcc acgactgact tcagggaccc gtgaatctcc gccttctgcg gcccgcccca
     5821 ctcgccgccg cgcgcctcga gctcgaagta gacgatgccc ttcacgccga ggttgagccc
     5881 cacgctgccg tcgggcgtct gcaggttcat ggggaagaag gccccgtcag cctggcgcag
     5941 ccgctcggca tagggcgcga gcacctggtc gaaatgggtc gagccgagct cctcctcgcc
     6001 ctcggccacc accgccaggt tcaccggcag cgtgccttcc gccttgagga tcgcctccac
     6061 ggcgttgagg aacgcccgct gcggcccctt ctggttggtc gcgccacgcg ccatcagcac
     6121 cgtgcccagc tcggtctcca ccaactcgcc ggcaaagggg tcggcgatgc gccagttcgg
     6181 ctcgatcggc tgtacgtcgt acatcatgta gaccagcaag gtcttctcgg cgccggcgtc
     6241 gaagtagccg aacacgccgg ggtggccgtc ggtctcgacg atggccacct cgccgaagcc
     6301 gagatcgcgc aggtcaccgg ccaccagttc agccatctcg cggatgccgt cgtcgacggc
     6361 gctgatcgag cgctgcctga cccagcgttg cagcgcgtcg acatgcgcct cgagatcgcg
     6421 gtcgatctgc gcgtacaccg cctcgtgccg ccccgcatac gccggtaccg cggcagcgtc
     6481 gaaggcctcg tcggtggtga actcgtgccg cggccgtgga tcgggccggt cgccggccag
     6541 tgcgggaggc gccaatgcgg cgctgagcgc cgcgacggcg agcagctgga gggtcggaac
     6601 gcgcagcagg gcttgcaggg gcatcttgtc tctccactcg agggaaggtc tgtccgggac
     6661 ggagcgtatg gccgcaccac cgcgcaccat gcctcctaat acttcagcca cgccgccatc
     6721 acaaggctgc gccgccggag cggcgaggaa ctcacttgcc tcccgcacga tctggactac
     6781 gcttgacgat caacacggcc ggctgcccag aggcgtaatc gatgcccaca catgctgcgc
     6841 gccccgggtg gtcagccgga ctgcgaatcc tgctg
//
LOCUS       DAOMPG010000120         6860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4746_length_6860_cov_4.227921, whole genome shotgun sequence.
ACCESSION   DAOMPG010000120 DAOMPG010000000
VERSION     DAOMPG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4746_length_6860_cov_4.227921"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLS34_07665"
     CDS             complement(<1..226)
                     /locus_tag="PLS34_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87382.1"
                     /translation="MIEFAREHALGLFVAAIMHAALIALLVVGLPARTVTPEPVARPP
                     VQAVAVSEADLRAAEQRQADARAAELRRQRE"
     gene            complement(230..679)
                     /locus_tag="PLS34_07670"
     CDS             complement(230..679)
                     /locus_tag="PLS34_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HRP87383.1"
                     /translation="MAAGKQKRRLMGEINVVPYIDVMLVLLIIFMVTAPLLTQGIEVE
                     LPKVGAAPMDPDKYIESDALVVSVDSQGRLYLNLVEPETPVSAEVAIERAGALLRTNP
                     ARPVLVRADARASYGDVAVAMRVLQDAGAAQIGMVTDPEELAAPGRR"
     gene            complement(683..1375)
                     /gene="tolQ"
                     /locus_tag="PLS34_07675"
     CDS             complement(683..1375)
                     /gene="tolQ"
                     /locus_tag="PLS34_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRP87384.1"
                     /translation="MQHDMSIVRLVLEASLVVQLVMLLLLLASLASWAVIIQKRRVLR
                     QARANADSFEKEFWSGGDLTGLYKRITGRGAGGAVTGMAGIFEAGFREFSRLRKQDDL
                     QASQIVEGSRRAMLVSQMRESDRLEQNLALLATVGSTSPYVGLFGTVWGIMNSFIALG
                     NVQQATLAMVAPGIAEALIATAMGLFAAIPAVIAYNRYADAVGRLESRYDTFMEEFST
                     ILQRHARRAVES"
     gene            complement(1383..1796)
                     /gene="ybgC"
                     /locus_tag="PLS34_07680"
     CDS             complement(1383..1796)
                     /gene="ybgC"
                     /locus_tag="PLS34_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRP87385.1"
                     /translation="MAGRISDRFSWPVRVYYEDTDLGGVVYYANYLRFLERARSEWLR
                     HLGVSQTRLLEEARLLFAITRVAIDYQKPARFEDELVVTVELARLRRASMEFRQAIHR
                     GGTDGELLCTAQVTAACLDADSMRPKPLPAHLFPE"
     gene            complement(1798..2859)
                     /gene="ruvB"
                     /locus_tag="PLS34_07685"
     CDS             complement(1798..2859)
                     /gene="ruvB"
                     /locus_tag="PLS34_07685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRP87386.1"
                     /translation="MIEHDRLVTAAAGKDEEALERAIRPRLLADYVGQASVRRQMEIF
                     IKAARQREEALDHVLIFGPPGLGKTTLAHIVANELGVHLRHTSGPVLERPGDLAAILT
                     NLEPRDVLFVDEIHRLSPVVEEILYPAMEDFQLDIIIGEGPGARSIKIDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVQRLEFYDDAELGRIVTRSAGILGVAIDAAGAHEIARRSRG
                     TPRIANRLLRRVRDYAQVEGDGRIDAGVATAAMDMLDVDPRGFDVMDRRLLLTIMEKF
                     DGGPVGVDSLAAAIGEERGTIEDVLEPYLIQQGLMMRTARGRMATRQAYLHFGLPAPS
                     RALDPETISLFEQSDGEQA"
     gene            complement(2864..3454)
                     /gene="ruvA"
                     /locus_tag="PLS34_07690"
     CDS             complement(2864..3454)
                     /gene="ruvA"
                     /locus_tag="PLS34_07690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRP87387.1"
                     /translation="MIGSLKGTLAAKHPPQLLIEVGGVGYEVEAPMSTFYQLPDTGQP
                     VHILTHLLVREDAHVLYGFASEAERQLFRSLIRISGVGARIALAILSGISAEAFVRCV
                     QSEDAASLTKIPGVGRKTAERLIVEMRDRLGQAGSSLAQAIPAGPSGSPVDEAFGALV
                     ALGYRPAEAQRLLKLVEDKASTTEDLIRAALQAAAR"
     gene            complement(3451..3954)
                     /gene="ruvC"
                     /locus_tag="PLS34_07695"
     CDS             complement(3451..3954)
                     /gene="ruvC"
                     /locus_tag="PLS34_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRP87388.1"
                     /translation="MTILGIDPGSQVTGFGVIAVEGRTMRYLASGCIRTRGGVMADRL
                     RVIFDGAGRLVDEYRPDEICIERVFMARNPDSALKLGQARAAALCGTFVHAVPVHEYA
                     AREIKQAVTGSGAAEKVQVAHMVRRLLAVRGPLAADAADALAVAICHAQLRSGRALRA
                     ALGGGGR"
     gene            complement(3988..4731)
                     /locus_tag="PLS34_07700"
     CDS             complement(3988..4731)
                     /locus_tag="PLS34_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRP87389.1"
                     /translation="MAGHSKWANIKHRKGAQDKKRGKIFTKLIREITSAARAGDPDPA
                     MNPRLRLAIDKAKAASVPKDTIDRAWKRGSGEIEGDDYQEVRYEGYAPGGVAVLVECL
                     TDNRNRTVGEVRHAFSKCGGNLGADGSVSYLFQHCGVLSYPAGTSEDRLMQAALEAGA
                     EDIVSNEDGSLDVITAPADFEAVRDALAAAGLEAEDAEVTMRAATGVAVDGDEADQVL
                     RLLELLDDLDDVQDVYSNAELSDDVLAGA"
     gene            complement(4799..5359)
                     /locus_tag="PLS34_07705"
     CDS             complement(4799..5359)
                     /locus_tag="PLS34_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HRP87390.1"
                     /translation="MLSRTLLSLALLAVAPLAAANGLDLNVNNKAVRVTMDFELSNNL
                     VVDGSWLHHQDKGDVYGLGLHVTGAATGGRDPLRAGLGGRLLRVDSDAGGREDGGVLP
                     LGGYVNYTLPEYNRFVLGGSLYYAPDVLSFGDVSTYWEYNAWAGYSVLRTGQVYLGIR
                     GIRAEFEDTPNVNMDTGLHFGLRLRF"
     gene            5453..>6860
                     /locus_tag="PLS34_07710"
     CDS             5453..>6860
                     /locus_tag="PLS34_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP87391.1"
                     /translation="MPAGDQALAAALAEARAQAAAGHQAAADRGLAIAALLEEIRAEP
                     EIRAAAVLYPLVESGAISSDAVEKRFDLAMRRRVEELQRLGSFGLPENWRTGTPLPPP
                     QAETLRKMLLAIVADVRLVLVRLADQLERLGRARTGDAELRERVAIETREIFAPLANR
                     LGIWHFKWQMEDLAFRYLEPETYRGIALYLKETREDREARIEEVLRVLRAELDKAGIH
                     GEIEGRPKHIYSIWRKMQRKHVGIEQVFDISAVRVLVDSVPECYAVLGQVHGLWPYIA
                     GEFDDYIANPKGNFYRSLHTAVMGPGNEPLEVQIRTREMHEHAELGVAAHWRYKEGGR
                     GDPAFEQKINWLRQLLAPGEDAEADRDLLDQLRTEIFEDRVYAFTPAGDVVDLPAGAT
                     PLDFAYQVHTSIGHHCRGARVNGRIVQLTHALKNGDKVEILTAKNSHPSRDWLSPQAG
                     YLVSPRSRSKVRSWFRQQD"
BASE COUNT         1091 a         2429 c         2280 g         1060 t
ORIGIN      
        1 cctcgcgctg ccgccgcagc tcggcggcgc gggcgtcggc ctggcgttgc tctgcggcgc
       61 gcaggtccgc ctcgctgacg gccaccgcct gcaccggcgg acgcgcgacc ggctccggcg
      121 tcacggtgcg cgccggcagc ccgacgacca gcaacgcgat gagcgcggca tgcatgatcg
      181 cggccacgaa caggccgagc gcatgctcgc gggcaaactc gatcaccggc tatctccgcc
      241 ccggggccgc gagttcctcg gggtccgtga ccatgccgat ctgcgcggcg ccggcatcct
      301 gcagcacgcg catcgccacg gccacgtcgc cgtagctggc ccgggcgtcg gcccttacca
      361 gcaccgggcg cgccgggttg gtcctgagca gcgcgccggc ccgctcgatc gcgacctcgg
      421 cgctcaccgg cgtttccggc tccaccaggt tgaggtagag ccggccctgg ctgtccaccg
      481 ataccaccag cgcgtctgac tcgatgtact tgtcgggatc catcggcgcc gcaccgacct
      541 tgggcagctc cacctcgatg ccctgcgtga gcaggggcgc ggtgaccatg aaaatgatca
      601 gcaggaccag catcacgtcg atgtagggca cgacgttgat ctcgcccatc agccggcgtt
      661 tctgcttccc ggccgccacg actcagctct ccacggcgcg gcgggcgtgc cgctgcagga
      721 tggtcgagaa ctcttccatg aaggtgtcgt aacggctctc cagccggccc acggcgtcgg
      781 catagcggtt gtaggcgatc actgccggga tggcggcaaa caggcccatg gccgtagcga
      841 tcagggcctc ggcgatgccg ggcgcgacca tcgccagcgt ggcctgctgc acgttgccga
      901 gcgcgatgaa ggaattcatg atgccccaca ccgtgccgaa caggccgacg taggggctgg
      961 tcgagccgac cgtcgccagc agcgcgaggt tctgttccag gcggtcggat tcacgcatct
     1021 gcgacaccag catcgcgcgg cgcgagccct cgacgatctg gctggcctgc aggtcgtcct
     1081 gcttgcgcag cctgctgaac tcgcggaagc cggcctcgaa aatgcccgcc atgccggtca
     1141 cggcgccgcc ggcgccgcgc ccggtgatgc gcttgtacag gcccgtcagg tcgccgcccg
     1201 accagaattc cttttcgaag ctgtcggcat tggcgcgcgc ctgccggagc acgcgccgct
     1261 tctggatgat gaccgcccac gaggcgagcg acgccagcag cagcaacagc atcaccagct
     1321 gcactaccag gctggcctcc aggacgaggc gtacgattga catgtcgtgt tgcatgcggg
     1381 ggttactccg gaaatagatg cgcgggcagc ggtttcggtc gcatggagtc cgcgtcgaga
     1441 caggcggctg tgacctgcgc agtgcacagg agttccccgt ccgtaccgcc gcgatggatg
     1501 gcctggcgga actccatgct tgcgcgccgc agcctggcga gctcgaccgt caccacgagt
     1561 tcgtcctcga accgggccgg cttctggtag tcgatcgcga cgcgggtgat ggcgaacagc
     1621 aggcgcgctt cctcgagcag gcgcgtctgg gagacgccga gatgacgcag ccactcgctc
     1681 cgcgcccgct ccaggaacct gaggtagttg gcgtagtaga ccacgccgcc gaggtcggtg
     1741 tcctcgtagt agacgcgcac cggccagctg aaccggtcgc tgatgcgccc cgccatgtca
     1801 ggcctgctcc ccgtcgctct gctcgaacag cgagatcgtc tccgggtcga gcgcgcggct
     1861 cggggcgggc aggccgaaat gcagataggc ctggcgggtc gccatgcggc cacgagcagt
     1921 gcgcatcatc aggccctgct ggatcaggta gggctcgagc acgtcctcga tggtgccgcg
     1981 ctcctcgccg atcgccgccg ccaggctgtc cacgccgacc ggcccgccgt cgaacttttc
     2041 catgatggtg agcaacagcc gtcggtccat cacgtcgaag ccgcgcgggt cgacgtcgag
     2101 catgtccatg gccgccgtcg ctacgccggc atcgatccgg ccgtcgcctt ccacctgggc
     2161 gtaatcgcgc acccgtcgca gcaagcggtt ggcgatgcgc ggcgtgccgc gcgagcggcg
     2221 cgcgatctcg tgggccccgg cggcgtcgat ggcgacgccg aggatgccgg cggagcgcgt
     2281 gacgatccgc ccgagctcgg cgtcgtcata gaactcgagt cgctgcacga tgccgaaacg
     2341 gtccctgagc ggcgaggtca gcaggccggc acgcgtggtg gcgccgacca gcgtgaaggg
     2401 cggcaggtcg atcttgatgg agcgggcgcc gggaccctcg ccgatgatga tgtcgagctg
     2461 gaaatcctcc atggcgggat agaggatctc ttccaccacc gggctgagcc gatggatctc
     2521 gtccacgaac agcacgtcgc gcggctccag gttggtcagg atggccgcca gatcgcccgg
     2581 ccgctccagc acgggaccgg aggtgtgccg caggtgcacg ccgagctcgt tcgccacgat
     2641 gtgcgccagc gtggtcttgc cgagcccggg cgggccgaag atcagcacgt gatcgagtgc
     2701 ttcctcgcgc tggcgcgccg ccttgatgaa gatttccatc tgccgccgga ccgacgcctg
     2761 gccgacgtag tccgccagca gccgtgggcg aatcgcccgc tccagcgctt cctcgtcctt
     2821 gcccgcggcg gccgtcacca gccggtcgtg ctcgatcatg acttcacctc gccgccgcct
     2881 gcagcgccgc gcgaatcagg tcctcggtgg tgctcgcctt gtcttcgacg agcttcagca
     2941 gccgctgcgc ctcggccggg cggtagccca gcgccaccag cgcaccgaag gcctcgtcca
     3001 cgggcgagcc cgacgggccg gccgggatcg cctgggcgag gctcgagccc gcctgcccga
     3061 gccgatcgcg catctcgacg atgagccgct cggcggtctt gcgcccgacg cccggaatct
     3121 tggtgagcga cgccgcatcc tcggactgca cgcagcgcac gaaagcctcg gcgctgatgc
     3181 ccgagagaat ggccagcgcg atgcgcgccc cgacgccgct gatgcggatc aggctgcgga
     3241 acagctgccg ctccgcctcg ctggcgaagc cgtacaggac gtgcgcgtct tcccgcacca
     3301 gcaggtgcgt gaggatatgc accggctggc cggtgtccgg cagctggtag aaggtggaca
     3361 tcggcgcctc gacctcgtag ccaaccccgc ccacctcgat gagcagctgc ggcggatgct
     3421 tcgccgccag cgtgcccttc agagaaccga tcatcggccg cctccgccca gtgccgcccg
     3481 cagcgcgcgc ccgctgcgca gctgagcgtg gcagatcgcg acagccagcg catccgccgc
     3541 atccgccgcg agcggaccgc gcaccgccag caggcgccgc accatgtggg ccacctggac
     3601 tttctcggcg gcgccgctgc cggtcaccgc ctgcttgatc tcgcgcgccg catactcgtg
     3661 caccggcacg gcgtgtacga atgtcccgca cagcgccgcc gcgcgcgcct ggcccagttt
     3721 cagtgcgctg tcggggttgc gcgccatgaa cacgcgctcg atgcagatct cgtcgggccg
     3781 atactcgtcc accagccgcc cggcgccgtc gaagatcacc cgcagccggt cggccatgac
     3841 accgccgcgc gtgcggatgc agccgctggc gagatatcgc atggtcctgc cctccacggc
     3901 gatgacgccg aagcccgtga cctgggaacc gggatcgatg ccgaggatcg tcaagccccc
     3961 ggtcatcgcc cgcgacccgg acaggcctca ggccccagcc agcacatcgt ccgacagctc
     4021 cgcgttgctg tagacgtcct gcacgtcgtc caggtcgtcg agcaactcca gcagcctcag
     4081 tacctggtcg gcctcgtcac cgtccaccgc aacgccggtc gcggcgcgca tggtgacctc
     4141 ggcgtcctcg gcctcgagac ccgcggccgc cagcgcgtcc cgtaccgcct cgaagtccgc
     4201 cggtgccgtg atcacgtcca gggagccgtc ctcgttgctc acgatgtcct cggcgcctgc
     4261 ctcgagagcc gcctgcatca ggcggtcctc gctcgtgccc gccggatagc tgagcacacc
     4321 acaatgctgg aacaggtagc tcacgctgcc gtcggcgccc aggttgccgc cgcacttcga
     4381 gaaggcgtgg cgcacttcgc cgaccgtgcg attgcggttg tcggtcaggc attccaccag
     4441 gaccgcaaca ccccccggcg cgtaaccctc gtagcgcact tcctggtagt catctccctc
     4501 gatctcgccg ctgccgcgct tccaggcgcg atcgatggtg tccttgggga cgctggcggc
     4561 cttggccttg tcgatggcca gccgcagccg cggattcatg gcgggatccg ggtcgcccgc
     4621 gcgcgccgcg ctggtgatct cgcggatcag cttggtgaag atcttgccgc gcttcttgtc
     4681 ctgggcgccc ttgcggtgct tgatgttcgc ccatttgcta tggcctgcca tggaatcccc
     4741 gcggggtgat gaaagccgcc aattttaccg tcggcccgga aagaccgtcc atgcaaccct
     4801 agaagcgcag ccgcaacccg aaatgcaggc cggtgtccat gttgacgttg ggcgtatcct
     4861 cgaactcggc acgaatgccg cggataccca ggtagacctg gccggtccgc aacacgctgt
     4921 aacccgccca ggcgttgtat tcccagtagg tgctgacatc cccgaaggac agtacgtccg
     4981 gtgcataata gaggctgccg ccgagcacga agcggttgta ctcgggcagc gtgtagttga
     5041 cgtacccgcc gagtggcagc acgccgccgt cctcgcgccc gcccgcatcc gagtcgacac
     5101 gcagcaggcg gccgccgagc ccggcgcgca gagggtcccg tccgcccgtg gccgcgccgg
     5161 ttacatgcag gccgagaccg tacacgtcgc ccttgtcctg gtggtggagc caggagccgt
     5221 ccacgaccag gttgttcgac agctcgaaat ccatggtgac ccgaacggcc ttattgttta
     5281 cgttcaggtc gagtccattg gccgcggcca gcggggcgac ggccagcagc gccagggaaa
     5341 gcaaggtccg gctcagcata aaacctccaa attcacatta actactgaat tctagtttct
     5401 gtattatata cgattcttga cgacaacaac tgtgactccc gcttcccgcc acatgcccgc
     5461 aggggaccag gcacttgccg ccgcactcgc ggaggcccgc gcgcaggcgg ccgcaggcca
     5521 ccaggcggct gccgatcgcg gcctcgccat tgccgcgctc ctcgaagaaa ttcgcgccga
     5581 gccggagatc cgcgccgccg ctgtacttta cccgctggtg gagtccggcg cgatcagcag
     5641 tgacgccgtc gaaaagcgct tcgacctggc gatgcgtcgc cgcgtcgagg aactgcagcg
     5701 gctggggtcc ttcggcttgc cggagaactg gcgcaccggt acgccgctgc cgccgccgca
     5761 ggcggagacc ttgcgcaaga tgctgctcgc catcgtggcg gacgtgcgcc tcgtgctggt
     5821 gagactggcc gaccagctgg agcgactggg gcgcgccaga accggcgatg cggagttgcg
     5881 cgaacgggtc gcgatcgaga cgcgcgagat tttcgccccg ctcgccaacc ggctcggcat
     5941 ctggcatttc aagtggcaga tggaagacct ggccttccgt tacctcgagc cggaaaccta
     6001 ccgcggcatc gcgttgtacc tcaaggaaac gcgcgaggac cgcgaagcac gcatcgagga
     6061 agtgctgcgc gtgctccgtg cggagctcga caaggccggc atccacggcg agatcgaggg
     6121 ccgcccgaag cacatctaca gcatctggcg aaaaatgcag cgcaagcacg tcggaatcga
     6181 gcaggtgttc gacatcagtg ccgtgcgcgt gctggtggac tcggttcccg agtgctacgc
     6241 ggtgctcggc caggtccacg gcctgtggcc ctacatcgcg ggcgaattcg acgactacat
     6301 cgccaacccg aagggcaatt tctaccgctc gctgcacacc gcggtgatgg gcccgggcaa
     6361 cgagccgctg gaagtacaga tccgcacgcg cgagatgcac gagcatgccg agctcggcgt
     6421 ggcggcccac tggcgctaca aggaaggcgg ccgcggcgac cccgccttcg agcagaagat
     6481 caactggctg cgccagctgc tcgcgccggg cgaggacgcc gaggcggatc gggacctgct
     6541 cgaccagctg cgcacggaaa tcttcgagga ccgggtgtac gccttcacgc cggcagggga
     6601 cgtggtggac ctgccggccg gcgccacgcc gctggatttc gcctaccagg tgcacaccag
     6661 catcggccat cactgccgcg gggcgcgggt gaacgggcgg atcgtgcagc tgacccacgc
     6721 gctgaagaac ggcgacaagg tcgagatcct gacggcgaag aactcgcacc cgagccgtga
     6781 ctggctgtcg ccgcaggccg gctacctggt gtcgccacgc agccgcagca aggtgcgcag
     6841 ctggttccgg cagcaggacc
//
LOCUS       DAOMPG010000121         6821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4780_length_6821_cov_4.205143, whole genome shotgun sequence.
ACCESSION   DAOMPG010000121 DAOMPG010000000
VERSION     DAOMPG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_6821_cov_4.205143"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLS34_07715"
     CDS             <1..252
                     /locus_tag="PLS34_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRP87392.1"
                     /translation="AVDRFLQLEREVASDQREIFAGFRKYAQKHRDIIARFGRFPHRN
                     AILGRRDTPEEAAWLAGGGERFGQSTPTDATGTHKTLPS"
     gene            complement(249..2051)
                     /locus_tag="PLS34_07720"
     CDS             complement(249..2051)
                     /locus_tag="PLS34_07720"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRP87393.1"
                     /translation="MNFKHARMIAVVFLLAACGVPTLDSQPVAPAESAAEQLDRLFAE
                     HWEATLELNPVLATFIGDHRFNDRFTVGISPEHRAATRALRQRSLAAVEAVDPEALDE
                     QRRLSRTLFLRDLRHELDGEQFPSHLLPIDQFRSTASFFVMLGSGSSAQPFTTVQDYE
                     NFLQRMDGFTDWVEQAIVNMREGVEAGVVQPAILMERTLPQLEAHIVEEVEQSLFYRP
                     VSAFPDTVPEGERARLRDAYAEAISGKLVPAYARLHDFIRDEYLPACRETYGLYALPD
                     GEAWYAYLIREHTTTELGAEEIHQVGLDEVARIHGEMIAIKQEVGFEGDLDAFFEHLN
                     TDPRYYFESREEIIAAHEALRQKADEAAPRLFHRSPRAGYEIRAVEEFRERSAAKGSY
                     QRPSTDGSRPGIFYVNTWNPTTRPRWDIEALFLHEAVPGHHFQRAFNLELEGLPAFRT
                     HGGVTAFAEGWGLYSESIPVGRAMGFYEDPYQRFGELNAELWRAIRLVVDTGLHAKGW
                     SRQQVLDYMYANSASLETRAVSEAERFMAIPGQALAYKIGQLKIAELRARAEAALGEA
                     FDIRDFHAQVLGQGDLPLDILEARIDAWLASRGR"
     gene            complement(2113..3480)
                     /locus_tag="PLS34_07725"
     CDS             complement(2113..3480)
                     /locus_tag="PLS34_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRP87394.1"
                     /translation="MQPPQSRRTRSIVEAAAILRLAGPLVANNLALAGMSFADTVMAG
                     RLGTTDPAAVAVGGSAWMVVFLLGLGTLMAVSPLVAHAYGAGRWEDAGGVLRQALWLS
                     QALAVGGFLLLLGAGPLLAAIGVDPTVVPLTSGYLFAMSFGLPGVFAFLAMRFMTEGV
                     GWTRPIMYAAAVGLVTNVFGNWVLMYGNLGFPRLGAVGCGMASAISMWSMFATMLLYL
                     LRSRRYAPFRLWGRLERPRWRAQREVLALGLPIAASVEAEAGLFAGTALIMATLGATQ
                     VAGHQIAINYAATMFMVPLAFHSATTIRVGQALGRGEARQARRAGWTGIGMCGLFMLM
                     SALVLFLFRDRIAAFYTTDPELLPLATALLTMAMVFQVSDGLQVGAAGALRGFKDTRV
                     PMLMNVASYWLLAFPMAWYAGVHARLGPIAVWVALIVGLTVTALLLNARFMRVSNRRV
                     AAAPL"
     gene            complement(3567..4220)
                     /locus_tag="PLS34_07730"
     CDS             complement(3567..4220)
                     /locus_tag="PLS34_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87395.1"
                     /translation="MNKSCLRAAALLAVVLLPLGWGSAAADSFAVGAKAGTTGLGLEA
                     TWRLTDAVNLRGGYYAFDYDTDLEEEGVEYDGDLRLRNAALFADWHPFGGAFRISAGG
                     VQSGNKFLGSADGELEVGDNTYTGQLEAEVSWSGLAPYLGIGFGNAMRGGRLSFSFDL
                     GVMFTGAPDVRLDGSVNDPALEDAFAEDLARERANLEDELSDAKYYPVVSLGFAYRF"
     gene            complement(4365..5837)
                     /locus_tag="PLS34_07735"
     CDS             complement(4365..5837)
                     /locus_tag="PLS34_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87396.1"
                     /translation="MLGNKTLALVLALAGVTMLDGREAGATTGPGWQEHVGRGYALQH
                     PAGWRVSETDDNRILIESGEGRYTLAAVALPNVRPDNLSPHMVQSCTGTRIDVRNFQR
                     VNPFSMRAAADWGDTYETSAIMYQPAGNGITLMCATIVAGKSQFASFAGTAARVVESM
                     KQVAPARAARGPATAGRDQDWTSAPRVQQRDPEEGSYTVSIPQGWSFRGGTGRVHVLE
                     TRQWFITSSPDGEITVIYGDVDVPRFVERDPLGMSPAGQWHTTQFSGNYFIWPYQHGL
                     QFARERAQNLFGSYADFAVDDEHQIDMTQSVRDAYAQSSPAMAYGSTSGLSAFTFSHQ
                     GQQFAGAYWATTTRLPDMGVWSLTQAVRIIATPDRVEEAVKLAMTMLPVPDPQWQQQQ
                     NAGAMRNQRIMTEAFADTNRIMRESYESRIASSERGTRDYIDGIWGTQRLQDPATGQR
                     YEMQHGSLSYWLHDDGTVVGTDTYDNPHPSHFRELLQVGN"
     gene            5976..>6821
                     /locus_tag="PLS34_07740"
     CDS             5976..>6821
                     /locus_tag="PLS34_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRP87397.1"
                     /translation="MERFNAWMETVNGVLWHDGVLVVVLLTGVVLTIWSRFGQYRALT
                     HGFQVIRGRYDEADDPGAINHFQALSAALSATVGLGNIGGVALAISLGGPGAVFWMWV
                     VGFFGMALKLTEVNLSMLYRNTDDPDNPHGGPMWVASKGLARIDPRLAGVGAFIGGLF
                     CITLLISTITGGNMFQAWNVAEITRSYFGVPGVASGILMALVVGVVIIGGIRRIGAVA
                     GRLVPFMVALYILAGSYVLILHAPELPGVFALIIKSAFGPTEAAGAFVGGTLGYAFLY
                     GMKRAL"
BASE COUNT         1089 a         2340 c         2351 g         1041 t
ORIGIN      
        1 gcggtggacc gcttcctgca actcgagcgc gaggtcgcct cagaccaacg cgagattttc
       61 gccggcttcc gcaagtacgc gcagaagcat cgcgacatca tcgcgcgctt cggccgtttc
      121 ccgcaccgca atgccatcct cgggcgccgc gacacgccgg aggaggccgc ctggctggcc
      181 ggcggcggcg agcgcttcgg ccagtcgacc ccgaccgacg ccaccgggac ccacaagacg
      241 ctgcccagct agcgcccccg cgaagccagc caggcgtcga tgcgcgcctc caggatatcc
      301 agcggcaggt cgccctgccc gagcacctgg gcatggaaat cgcggatgtc gaaagcctcg
      361 cccaatgccg cctcggcacg ggcccggagt tccgcgatct tcagctgccc gatcttgtag
      421 gccagcgcct ggccggggat ggccatgaag cgctccgcct ccgagaccgc gcgcgtctcc
      481 agcgacgccg agttggcata catgtaatcg agcacctgct ggcggctcca gcccttggcg
      541 tgcaggccgg tgtccaccac caggcggatg gcgcgccaca gctcggcgtt gagctcgccg
      601 aagcgctggt aagggtcctc gtagaagccc atggcgcggc cgaccggaat cgactcggaa
      661 taaaggcccc agccctccgc gaaggcggtc acgccgccat gcgtccggaa agccggcagg
      721 ccctcgagct cgagattgaa ggcgcgctgg aaatgatgtc cgggaaccgc ctcgtgcagg
      781 aacagcgcct cgatatccca gcgtggccga gtggtcggat tccacgtgtt gacgtaaaag
      841 atgccggggc ggctgccgtc ggtggacggc cgctggtagg aacccttggc cgcggatcgc
      901 tcgcggaact cctccacggc gcgaatctcg tagccggccc gcggcgagcg atggaatagc
      961 cgcggcgcgg cctcgtcggc cttctggcgc agtgcctcgt gcgcggcgat gatctcctcg
     1021 cgggactcga agtagtagcg tggatcggtg ttgagatgct cgaaaaatgc gtcgaggtca
     1081 ccctcgaagc cgacttcctg cttgatggcg atcatctcgc catgaatgcg cgcgacctcg
     1141 tcgaggccga cctggtggat ctcttcggcg ccgagctccg tggtcgtgtg ctcgcggatc
     1201 aggtaggcgt accaggcctc gccgtcgggc agcgcgtaca gcccgtaggt ttcgcggcag
     1261 gcgggcaggt attcgtcgcg aatgaaatca tgcagccgcg cgtaggccgg cacgagcttg
     1321 ccgctgatcg cctctgcgta ggcatcgcgc aggcgcgcgc gctcaccctc cggcacggtg
     1381 tcggggaagg cactcaccgg gcggtagaac aggctctgct cgacctcctc gacgatgtgc
     1441 gcctcgagct gcggcagcgt ccgctccatt aggatcgccg gctgcacgac gccggcctcg
     1501 acgccctcgc gcatgttcac gatggcctgc tccacccagt cggtgaagcc gtccatgcgc
     1561 tggaggaaat tctcgtagtc ctggaccgtg gtgaagggct gcgcgctgga gcccgagccg
     1621 agcatgacga agaaactggc ggtgctgcgg aactggtcga tgggcagcag gtggctgggg
     1681 aattgctctc cgtccagctc gtggcgaaga tcccgcagga acagcgtgcg gctgaggcgg
     1741 cgctgctcgt ccagcgcttc cgggtcgacg gcctcgaccg ccgcaagact gcgctgccgc
     1801 aatgcgcgcg tcgccgcgcg atgctccggg ctgatgccga cggtgaagcg gtcgttgaaa
     1861 cgatggtcgc cgatgaaggt ggcgagcacc ggattgagct ccagcgttgc ctcccagtgc
     1921 tcggcaaaca gccgatcgag ctgttcggcc gcggactcgg ccggcgcgac gggctgcgaa
     1981 tcgagcgtcg gcacgccgca agcggccagc aggaaaacga cggcgatcat ccgggcgtgc
     2041 ttgaaattca tcggcgagaa ctctcctgtg attccggggc ggccggcgcc gcagcgcagc
     2101 gccggctgcc aactatagcg gtgcggcggc gacgcggcga ttcgacaccc gcatgaagcg
     2161 tgcattcagc agcagcgccg tcaccgtcag gccgacgatc agcgcgaccc acacggcgat
     2221 cgggcccagc cgggcgtgga cgccggcata ccaggccatc gggaaggcca gcagccagta
     2281 gctggcgacg ttcatcagca tcggaacgcg ggtgtccttg aagccgcgca gggcgccggc
     2341 cgcgcccacc tgcaggccgt cggagacctg gaacaccatc gccatggtca gcagcgcggt
     2401 cgccagcggc aacagctcgg ggtccgtggt gtagaaagcg gcaatgcggt cgcgaaacag
     2461 gaacagcacc agcgcggaca tcagcatgaa caggccgcac atgccgatcc cggtccagcc
     2521 ggcgcgccgc gcctggcgcg cctcgccccg gccgagcgcc tgcccgacac ggatggtggt
     2581 cgccgagtgg aaggccagcg gcaccatgaa catggtggcg gcgtagttga tggcgatctg
     2641 gtggcccgcc acctgggtgg cgccgagcgt ggccatgatc agcgcagtgc cggcgaacag
     2701 cccggcctcg gcctccacgc tggcggcgat gggcaggccc agcgccagca cctcccgctg
     2761 cgcgcgccag cgcggccgct ccagccggcc ccacaagcgg aacggggcat agcgacgcga
     2821 gcgcagcagg tacagcagca tggtggcgaa catgctccac atggagatgg cgctggccat
     2881 gccgcagccg accgcgccga gccgcggaaa gccgaggttg ccgtacatca acacccagtt
     2941 gccgaacacg ttggtcacca ggccgacggc cgccgcgtac atgatcggac gcgtccagcc
     3001 gacgccctcg gtcatgaagc gcatggcgag aaaggcgaac acgccgggca ggccgaagga
     3061 catcgcgaac aggtagccgg aagtcagcgg cacgaccgtg gggtcgacgc cgatcgccgc
     3121 cagcagcggg cccgcgccca gcaacagcag gaagccgccg actgccagcg cctggctcag
     3181 ccagagcgcc tggcgcagca cgccgccggc gtcctcccag cggcccgcgc cgtaagcgtg
     3241 cgccaccagc gggctcacgg ccatgagcgt gcccaggccg agcaggaaca ccaccatcca
     3301 cgccgagccg cccaccgcca ccgcggcggg gtcggtcgtg ccgagcctgc ccgccatgac
     3361 ggtgtcggcg aagctcatgc ccgcgagcgc aagattgttg gccaccaggg ggccggccag
     3421 ccgcaggatg gcggcggcct cgacgatgct gcgggtgcgg cggctctgcg gcggttgcac
     3481 ggtcggatcc cggcaaatgg gtcccgcatt ctacgccgcc gccggcgccg cgcgggcggc
     3541 cggctcagcg gcgtccgtgc cggcggtcag aagcggtagg cgaagcccag gctgaccacg
     3601 gggtagtact tggcgtccga cagctcgtcc tcgaggttgg cacgctcccg tgcaaggtcc
     3661 tcggcgaagg cgtcctccag cgccgggtca ttcacggagc catccaggcg cacgtccggc
     3721 gcgccggtga acatcacgcc gaggtcgaag gaaaacgaca gccttccgcc acgcatggcg
     3781 ttgccgaagc cgatgcccag gtagggcgcc aggccgctcc agctgacttc ggcctccagc
     3841 tggcccgtgt aggtattgtc gccgacctcc agctcgccgt cggccgaacc caggaacttg
     3901 ttgccggact gcacgccgcc ggcgctgatc cggaacgcgc ccccgaaggg gtgccagtcg
     3961 gcgaacagcg ctgcattgcg cagccgcagg tcgccgtcgt actcgacgcc ttcttcctcg
     4021 aggtccgtgt cgtaatcgaa ggcgtagtag ccgccccgca ggttgaccgc gtcggtcagc
     4081 cgccaggtgg cctcgagccc cagccccgtg gtgccggcct tggcgccgac ggcgaaggaa
     4141 tccgccgcgg ccgagcccca gccgaggggc agcaggacga cggcgagcag ggcggcggca
     4201 cgaaggcagg acttgttcat gcgctgaact tccggtggca tggcgcggtt gcctgagcct
     4261 gtatcggcag cgcccggcgg cgacttgaac agccggggtg acggggcgac gaccccgatc
     4321 cgcagcgacg cggcacgatg gccgcggccg ccggcgcctc gggctcagtt tccgacttgc
     4381 agcagctcgc gaaaatggct cgggtgcgga ttgtcgtagg tgtcggtccc gaccaccgtg
     4441 ccgtcgtcgt gcagccagta ggacagcgat ccgtgctgca tctcgtatcg ctggccagtg
     4501 gccggatcct gcaggcgctg cgtgccccag atgccgtcga tgtagtcgcg ggtcccgcgc
     4561 tcggacgagg cgatccggct ctcatacgac tcccgcatga tgcggttcgt gtccgcgaaa
     4621 gcctcggtca tgatgcgctg gttgcgcatg gcgccggcat tctgctgctg ctgccactgt
     4681 gggtcgggca cgggcagcat ggtcatggcc agtttgaccg cctcctcgac acgatccggc
     4741 gtggcaatga tgcgaacggc ctgcgtcagg ctccagacgc ccatatccgg aagacgggtg
     4801 gtcgttgccc agtaggcgcc ggcgaactgc tggccctggt ggctgaacgt gaaggccgag
     4861 agcccggagg tcgagccgta cgccatcgcc ggactgctct gggcataagc gtcccgcacg
     4921 gactgcgtca tgtcgatctg gtgctcgtcg tccaccgcga aatcggcgta ggaaccgaac
     4981 aggttctgcg cccgctcgcg ggcgaactgc agcccgtgct gatacggcca gatgaagtag
     5041 ttgccggaaa actgggtggt atgccactgg cccgcagggc tcatccccag cggatcacgc
     5101 tccacgaacc gcggcacgtc gacgtccccg tagatgaccg tgatctcacc gtcaggactc
     5161 gaagtgatga accactgccg cgtctccagc acgtgcaccc ggcccgtccc gccccgaaag
     5221 ctccagccct gcggaatgct tacggtgtac gaaccttcct ccgggtcgcg ctgctgcacc
     5281 cggggcgccg aggtccagtc ctggtcgcgt cccgcggtgg caggtcctcg ggcggcgcgt
     5341 gccggggcca cctgcttcat ggactcgacc accctggcgg cggtgccggc gaacgatgcg
     5401 aactgcgact tgccggccac gatggtggcg cacatcaggg tgatgccgtt gccggccggc
     5461 tgatacatga tcgccgaggt ctcgtaggtg tctccccagt ccgctgcggc acgcatgctg
     5521 aacgggttca cgcgctggaa gttccgaacg tcgatccggg tgccggtgca ggactgcacc
     5581 atgtgcggac tcaggttgtc gggacgcacg ttcggcaggg cgaccgcggc cagcgtatag
     5641 cgtccctcgc ccgactcgat gagaatgcga ttgtcgtcgg tttcggacac ccgccaaccc
     5701 gccggatgct gcagggcgta tccgcgcccg acatgctcct gccagcctgg accagtcgtc
     5761 gccccggcct cccggccgtc gagcatcgtc acgcctgcaa gcgccagcac cagggccagc
     5821 gttttatttc ccagcatcat cacccccttg gtatgccggc gtcatgccga tgcacatgtc
     5881 ggccggaagt ttagcagccg gtcttgtcag cacgattgtc gcatggccat gatggcggcc
     5941 tcgcgcgccc cggcgcgccc agcgggatgc gccggatgga gcgattcaac gcctggatgg
     6001 agaccgtcaa cggcgtgctg tggcacgacg gcgtgctggt cgtggtactg ctcaccggcg
     6061 tggtactcac catctggagc cgcttcggcc agtaccgcgc cctcacgcac ggcttccagg
     6121 tgattcgtgg ccgctacgac gaggccgacg acccgggcgc cattaaccac ttccaggccc
     6181 tgtcggcggc gctgtccgcc acggtggggc tcggcaacat cggtggtgtc gcgctggcga
     6241 tctcactggg cggcccgggc gcggtgttct ggatgtgggt ggtgggtttc ttcggcatgg
     6301 cgctcaagct gaccgaagtg aacctgtcga tgctgtatcg caatacggat gaccccgaca
     6361 acccgcacgg cgggccgatg tgggtcgcca gcaaggggct ggcgcgcatc gatccgcgcc
     6421 tcgccggcgt cggggccttc atcggcgggc tgttctgcat cacgctgctg atctccacca
     6481 tcaccggcgg caacatgttc caggcgtgga acgtcgccga gatcacgcgc agctatttcg
     6541 gcgtgccggg cgtcgcctcg ggcatcctga tggcgctggt cgtcggcgtg gtcatcatcg
     6601 gcggcatccg gcgtatcggc gcggtggccg gccggctggt gcccttcatg gtcgcgctct
     6661 acatccttgc gggcagctac gtgctgatcc tgcacgcgcc cgaacttccc ggggtgttcg
     6721 cgctgatcat caagtcggcg ttcggaccga ccgaagcggc cggcgcattc gtcggcggca
     6781 cgctgggcta cgccttcctg tacggcatga agcgcgccct g
//
LOCUS       DAOMPG010000122         6768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4825_length_6768_cov_4.712349, whole genome shotgun sequence.
ACCESSION   DAOMPG010000122 DAOMPG010000000
VERSION     DAOMPG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4825_length_6768_cov_4.712349"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1756
                     /locus_tag="PLS34_07745"
     CDS             350..1756
                     /locus_tag="PLS34_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRP87398.1"
                     /translation="MSKSSVQLFGQHVDLWALILAAVDFALLCAAVHLAGLIQHAGDL
                     ESLKAAVGAPWLASLVFAGVALGALIAMGLYNRRVDARTSAIIARMVVGLVIGASAMT
                     ILMFFFEGLKLERGTLALSVSLALPLLAVSRVLFVQVLGRERFARRVLVYGIGAKAAE
                     LQNLRVRQDLRGFQVVGYVPTEGCTPKVPAEWRINPGDDLREFVLEEDVDEVVVAMED
                     RRRGLPVQQLLDCRLAGVPVSDVLSFMERESGKVELSMLYPSWLIYSEGINSRGVSRL
                     LTRVLDLMAAGLLLLLAAPLMLLTALAIWVESGFKGNVLYRQTRVGLEGQLFTLLKFR
                     SMREDAEKDGAKWAQKNDPRVTHVGRFIRTTRIDELPQLLNVLAGSMSFVGPRPERPE
                     FVAQLSQKIPYYRERHCVKPGITGWAQVCYPYGASEQDALEKLQYDLYYVKNKSVLFD
                     LMILLQTAEVVLWRKGSR"
     gene            complement(1826..2467)
                     /locus_tag="PLS34_07750"
     CDS             complement(1826..2467)
                     /locus_tag="PLS34_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRP87399.1"
                     /translation="MKSFKPAGSLAVGLACMGLAGCFGGDSETDAGGNSPPTISGTPP
                     TSATVGRQWSFQPSISDPDGDALTVTADNRPPWLSLDGSTGRMQGTPQEGDVLTWSGI
                     RLTVTDGMASASLPGFSIVVVPQTAHSGTATLSWSPPTERADGSPIGDLAGYEVLYGQ
                     ASRNYDTVIELNNPGLTRYMIENLGPGTWYFAVKAVTTDGLESAPSQEVSKTI"
     gene            complement(2885..3424)
                     /locus_tag="PLS34_07755"
     CDS             complement(2885..3424)
                     /locus_tag="PLS34_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRP87400.1"
                     /translation="MIKKFLPLLALALGLAAHTASADVIYTSDNFLDDPLYMNNPLFS
                     AGDSYSYTHDMTAEGYVPGSAILDGQLSLYLSDDHDFCLFGCPGETALVVGLGIFELV
                     NAAVPQEIEIGGLGLLSIWFDGLYSYTITAVTGDFYFHGSSLTITAESVPEPATLALL
                     GMGLLGAGALRRRRSAAAT"
     gene            complement(3790..5640)
                     /locus_tag="PLS34_07760"
     CDS             complement(3790..5640)
                     /locus_tag="PLS34_07760"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRP87401.1"
                     /translation="MATWNSPLKTRCSALIIAFLLTLGGCGGGESDGGSSVTPPPAGT
                     NPPPSGGTPPPSGGTTENHVTGSVGDGPIVGARLRVFARNGALLTETTSGDTADYDLV
                     ITTQDNNYALTLQADRGIDLVTGGTPDFRLVSAISRPSTRTISNLNPFSTLIYETARR
                     NGGISDSSVSAARHAVTTRYGFGLDTALLADPVTTPITGDNVAMVVKTSETLGEMIRR
                     TRDAMNATGQNLNGDAVVAALSADLVDGWLDGMGAYESNSRLAAVANVASAAVLVEAM
                     SNELQVYGNNATDAMDLSIRQVRPDAPQTASTRNVTIPATTFRQAARVLRAAQIVDPD
                     PVIAATLEVIETAEADLEPEILAARLPSGSRGALRRATTSTAYASDDVIRAVNAQARG
                     NSSTDPEPDPDPTPPPPDPDPDPTPPPPDPDPEPDPDPTPPPPANNPPVISGSPNTAL
                     VVGTPWSFTPTASDPDGDSLTFSVAGAPNWMYFNSSTGRLYGTPSSANVGTYTNIRIS
                     VSDGQATTTLPAFSLTVTEPQPSTGAATVSWTPPTERVDGSPIGTISSYRIYYGRNSA
                     QLDQAVNVSGGLTSFHIDNLVQGTWYFSVTATCADGLESARSAMVSKTIP"
     regulatory      complement(5812..5900)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(6058..6143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(6460..>6768)
                     /locus_tag="PLS34_07765"
     CDS             complement(6460..>6768)
                     /locus_tag="PLS34_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRP87402.1"
                     /translation="WLRNGGDSQVLNCGCGHGYSVREVVQAVERLTGEPLPVREDARR
                     PGDPPTLVANANRIRELLGWTPQLDDLDKIVASSLAWERNMRYPPPPAPPPGRTTPSS
                     "
BASE COUNT         1023 a         2010 c         2498 g         1237 t
ORIGIN      
        1 ttcgccgcga attccgattg gaatcagtct cggtctgcta tattgcatct ataagctcgg
       61 tctaattcgc gacaatacaa tatgttaaac gtgatttttg cacccgtgtc gcatgcgcgg
      121 agccaggacg gtaaagcttt ggtaagggtt cgcgagttaa aatgcctgtc cccgggcaaa
      181 ccgcaccggg cagcgtgacg aggatcacag cgtggccgcc agggaaccac ttagagtgga
      241 gaaatcgagt gaaaccggcc ccccaacggt aactcccatc ctgaagccag tgaattccca
      301 tatcgaatcc ctgcagagca cgccgccccc tgagcggcgg cgctggcgcg tgtccaagtc
      361 atcggtgcag ctgttcgggc agcacgtcga tctctgggcg ctgattctcg cggcggtgga
      421 tttcgcgctg ctgtgcgcgg ccgtgcacct cgccgggctg atccagcacg ccggtgacct
      481 ggagagcctg aaagcggccg tcggcgcgcc gtggctggcc tcgctggtgt tcgccggcgt
      541 ggcgctcggg gcgctcattg ccatggggct gtacaaccgg cgggtggacg cacgcacctc
      601 ggcgatcatc gcccggatgg tggtgggact ggtgatcggc gcctcggcga tgaccatcct
      661 gatgttcttt ttcgaggggc tcaagctgga acgtggcacg ctggccttgt cggtctcgct
      721 ggctttgccg ctgctggcgg tgtcgcgcgt gctgttcgtc caggtgctgg gccgcgagcg
      781 cttcgcccgc cgggtgctgg tctacggcat cggcgccaag gcggcggagt tgcagaatct
      841 ccgcgtgcgg caggacctgc gcggcttcca ggtggtgggc tacgtgccga ccgagggctg
      901 cacgccgaag gtgccggccg agtggcgcat caacccgggc gacgacctgc gcgagttcgt
      961 gctggaagag gacgtggacg aggtcgtggt ggcgatggaa gaccggcgcc gcggcctgcc
     1021 cgtgcagcag ctgctggact gccggctggc gggcgtgccg gtcagcgacg tgctcagctt
     1081 catggagcgc gagagcggca aggtcgagct gtccatgctc tacccgagct ggctgatcta
     1141 ctccgagggc atcaactcgc gcggcgtgtc gcggctgctg acgcgcgtgc tggacctgat
     1201 ggcggccggc ctgctgctgt tgctggcggc gccgctgatg ctgctgaccg cgctggcgat
     1261 ctgggtggaa tccggcttca agggcaatgt gctgtaccgc cagacgcggg tcggcctcga
     1321 ggggcagctc tttacgctgc tgaagttccg cagcatgcga gaggacgcgg aaaaggacgg
     1381 cgccaagtgg gcgcagaaaa acgacccgcg cgtcacgcac gtcgggcgct tcattcgcac
     1441 tacgcgtatc gacgagctgc cgcagctgtt gaacgtgctg gccggcagca tgagcttcgt
     1501 cgggccgcgc ccggagcggc cggagttcgt cgcgcagctg tcgcagaaga ttccctatta
     1561 ccgcgagcgc cattgcgtga agccgggtat caccggctgg gcgcaggtct gctatcccta
     1621 cggcgcctcc gagcaggacg cgctggagaa gctgcagtac gacctgtact acgtgaagaa
     1681 caagtcggtg ctgttcgacc tgatgatcct gctgcagacc gccgaggtgg tgctgtggcg
     1741 gaagggatcg cggtagtcga agcgacgtgc gcatgagcaa aggccggcgc ccgcaaggac
     1801 gccggccttt ttcgtgcggc cggggtcaga tcgtcttgct gacttcctgc gagggcgcgc
     1861 tttccagccc gtcggtggtc accgccttga cggcgaagta ccaggtgccg ggaccgagat
     1921 tctcgatcat gtagcgcgtc aggccgggat tgttcagctc gatcaccgtg tcgtagttgc
     1981 gcgaggcctg gccgtagagg acctcgtagc cggccaggtc gccgatcggc gagccgtcgg
     2041 cgcgctcggt cggcgggctc cagctcagcg tcgcggtgcc cgaatgggcg gtctgcggca
     2101 ccacgacgat cgagaagccg ggcaggctgg cgctggccat gccgtcggtc accgtgaggc
     2161 gaatgccgct ccaggtgagc acgtcgcctt cctggggggt gccctgcatc cggccggtag
     2221 agccgtcgag gctgagccac ggcggccggt tgtccgccgt gacggtgagc gcgtcgccgt
     2281 ccgggtcgga gatggacggc tggaaagacc actgacggcc gacggtggcg ctggtgggcg
     2341 gcgtaccgct gatggtcggc gggctgttgc cgccggcgtc ggtttcggaa tcgccaccga
     2401 aacagccggc aaggcccatg caggccaagc cgaccgccag cgatccggcg ggtttgaatg
     2461 atttcatgtt ctggttcatg gcggggggag ccaatcctca aaagtgtgat ttgggttgtc
     2521 gaaaaaactg cgcccttgta gcaggggacg ttaagtcaaa caacaggtac tagccgaaag
     2581 ttcgtttaat aaacagaaac ttgcgccgga accgcaccgt ttcgccgggg cattaacctt
     2641 ataaatcggc agcttgtggc gaatctttaa ccacgcgtcg aggagacgtt gtggggtttt
     2701 tctagacttc gcatgaagag tggcctgcag gtgtttgccg cattgcggaa aattgcaggt
     2761 cgcggcggcc tttcaccggg atggaagggg gcgcttgcgg gttgatttgc gcaacgacgc
     2821 gcgcaaaaaa aacggcgccc cgaggggcgc cgcatcgcta ttccaggtcg aggcccggcg
     2881 cggctcaggt ggccgcggcg ctccggcggc ggcgcaacgc ccccgccccg agcagcccca
     2941 tgccgagcag cgccagggtc gccggctccg gcacggactc ggccgtgatg gtcagcgacg
     3001 agccatggaa gtagaagtca ccggtgaccg cggtgatggt gtaggagtac aggccgtcga
     3061 accagatgct cagcagaccc agcccgccga tctcgatctc ctgcgggacc gcggcattga
     3121 ccagctcgaa gatgcccagc ccgaccacca gcgcggtttc gccggggcag ccgaacaggc
     3181 agaaatcgtg gtcgtcgctc aggtacagcg agagctggcc gtcgaggatc gcgctgccgg
     3241 ggacgtagcc ttcagcggtc atgtcgtggg tgtagctgta cgagtccccc gcgctgaaca
     3301 gcggattgtt catgtagagc gggtcgtcga ggaaattgtc actcgtgtag atgacgtcgg
     3361 ccgaagccgt gtgggccgcg aggccgagcg ccagggccag cagcggcagg aatttcttga
     3421 tcatatcggt actcccttat ccggagaatc acagtcggtg cgtccatgct gccgcgaccc
     3481 ggcaagttcc cccctgccca cgacaacact tgccttgtac aacttgcaag ttttacgcca
     3541 gctcggtgga agggattgga aaggcgtgtc aagacggggg cttgcgagtc tcggtgggtt
     3601 cgggcgtcgg cttgtacagg tttttccgct ccgtttgtgt aagatttctc gacgtgacct
     3661 gcgtcacagt tttgggtgcg tggcctggcg gcctgccagc gtcggcccgc cgaaaaaaag
     3721 cgcgggccgc tttcgcgacc cgcgctcggg aaccggcccc gaggggccgc tgcactgctc
     3781 ccgggatcgt tacgggatgg tcttgctcac catggccgac cgggcgctct cgagcccgtc
     3841 cgcgcaggtc gcggtgaccg agaagtacca ggtcccctgt accaggttgt cgatatggaa
     3901 gctggtcagg ccgccgctga cgttgacggc ctggtcgagc tgcgcgctgt tgcggccgta
     3961 gtagatccgg tagctgctga tcgtgccgat cggcgagccg tccacgcgct cggtcggcgg
     4021 cgtccagctc accgtggccg cgccggtcga gggctgcggc tcggtcaccg taagcgagaa
     4081 ggccggcagg gtggtggtcg cctggccgtc gctcaccgag atgcggatgt tggtgtaggt
     4141 gccgacattc gcgctgctcg gcgtgccgta cagccgcccg gtggacgagt tgaagtacat
     4201 ccagttcggc gcgccggcga ccgagaaggt caggctgtcg ccgtcggggt cggaggcggt
     4261 gggcgtaaag ctccacggcg tgccgacgac cagcgccgtg ttcggcgagc cggagatcac
     4321 cggcggattg ttggccgggg gcggcggcgt cgggtccgga tccggctcgg gatcggggtc
     4381 cggcggcggc ggggtcgggt cggggtccgg atcgggcggc ggcggggtgg gatcggggtc
     4441 cggctccggg tccgtcgagc tgttgccgcg cgcctgggcg ttgacggctc tgatgacgtc
     4501 gtcggaagcg taggcggtgc tggtggtcgc gcgacgcagg gcgccgcgtg agccggacgg
     4561 caggcgggcg gcgaggatct cgggctccag gtccgcctcg gcagtctcga tgacctccag
     4621 ggttgccgcg atcaccgggt cgggatcgac gatctgggcg gcgcgcagca cgcgggctgc
     4681 ctgccggaag gtggtcgccg ggatggtgac gttgcgggtg ctggcggtct gcggcgcgtc
     4741 gggacgcacc tggcggatgg acaggtccat cgcgtcggtg gcgttgttgc cgtagacctg
     4801 cagctcgttg ctcatggcct cgaccagcac ggcggcgctg gcgacgttgg cgacggcggc
     4861 gaggcgcgag ttgctctcgt aggcgcccat gccgtcgagc cagccgtcca ccaggtcggc
     4921 ggacagcgcg gccaccacgg cgtcgccgtt gaggttctgg ccggtggcgt tcattgcgtc
     4981 gcgggtgcgg cggatcattt cgcccagtgt ttcgctggtc ttgacgacca tggcgacgtt
     5041 gtcgccggtg atcggggtgg tgaccgggtc ggccagcagg gcggtgtcga ggccgaagcc
     5101 gtagcgggtg gtaacggcgt ggcgggcggc ggagacgctc gagtcgctga tgccgccgtt
     5161 gcggcgcgcg gtctcgtaaa tcagcgtgct gaaggggttc aagttgctga tcgtgcgcgt
     5221 cgaggggcgg ctgatggcgg acaccaggcg gaagtcgggc gtgccgccgg tgaccaggtc
     5281 gatgccccgg tcggcctgca gggtcagcgc gtagttgttg tcctgggtgg tgatgaccag
     5341 gtcgtagtcg gcggtgtcgc cgctggtggt ctcggtgagc agcgcgccgt tcctggcgaa
     5401 gacgcgcagg cgcgcgccga cgatcgggcc gtcgccgacg ctgccggtga cgtggttctc
     5461 ggtggtcccg ccgctcgggg gcggggtgcc gccgctcggc gggggattcg tgccggccgg
     5521 cggcggagtg acggaggagc cgccgtcact ctcgccaccg ccgcacccgc cgagagtcag
     5581 caggaaagcg atgatcagcg cgctgcagcg cgtctttagt ggtgaattcc aggtagccat
     5641 ggcgaaacct cgcttcgcaa catggccacg gaaaagatca actcacgggt agcagtgctt
     5701 tgcaaaaagc gcggtgtccc gcgccggatc ggacgcgccg gaggtggaaa gtggcaggcc
     5761 agccgtgtgc ggctgcgcaa ccgttggtat gacgaatgtc ttcccatgtt ttctggcggc
     5821 cccgctatcg tagaacccgg aggttcctta gaccggaagc tttgcgtcct cacctttcag
     5881 tgagtttgcc tttttcagtg gcctcgactg tgtcgtgtac tccccccgtc gccggggacg
     5941 tgctgacagg acaaaccgcc gagaggcgtg gccgtagcgt ggccgtccgg gagtcggttg
     6001 cgcagggagt tgccgaggcg ggcgcacgcc gggggagggg atggcgcgtc ccgggtcttt
     6061 ggcaaccccg ctgtcgtagg acctcgtccc ttagaccggt ggctttgcgt ccccgccttt
     6121 cgacgggttt gcccttgtca gtgggcgtag ttctacgcgt ggcgcggcgg gcccgcaata
     6181 ggtatttgac gtttgccggc ggcgccgtgc gggaagtgtg acttgcgtca cacccccgcg
     6241 ggtcggaccc gcggaagtaa ttgataataa atggaaaact tcgtagaagg ggtaattctg
     6301 aagccttaga acgctgattt ttgggccaaa aatgagctgt aagggcagtt gggaatcggg
     6361 gcggccctgg cgggttgtcg taagagcgcc ggaaatgacc ggtttccggt gttctaaggc
     6421 gtgcggaagg cgggttttgg gctggttttt ctcgattcgt caggagcttg gcgtggtccg
     6481 acccggtggt ggggccggtg gtggggggta gcgcatgttg cgctcccagg ccagcgagga
     6541 ggcgacgatc ttgtcgaggt cgtcgagctg cggcgtccag cctagcagct cgcggatgcg
     6601 gttggcgttc gcgaccagcg tcggcgggtc gccgggccgg cgtgcgtcct cgcgcaccgg
     6661 cagcggctcg ccggtgagcc gctccaccgc ctgcaccact tcgcgcacgc tgtagccgtg
     6721 gccgcagccg caattgagca cctggctatc cccgccgttg cgcagcca
//
LOCUS       DAOMPG010000123         6749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4844_length_6749_cov_4.497909, whole genome shotgun sequence.
ACCESSION   DAOMPG010000123 DAOMPG010000000
VERSION     DAOMPG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4844_length_6749_cov_4.497909"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLS34_07770"
     CDS             <1..334
                     /locus_tag="PLS34_07770"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HRP87403.1"
                     /translation="IAPGASGDLALWRRDDGVDTELVDGVEDLWLRYGEDLNNDGAPN
                     VYVEAAAVGNWSRVRSLRISLLLASNEDNVTEGAQPVDFRGAVVVPADNRFRQVLTTT
                     VGLRNRLP"
     gene            347..841
                     /locus_tag="PLS34_07775"
     CDS             347..841
                     /locus_tag="PLS34_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HRP87404.1"
                     /translation="MKAKQPEQSLTRQRERGSALAVALIFLLLMTLLGISAMRGSNMQ
                     ERMAGNLRDRNLAFQASEAALRGGETWLLNIVNQQTADLNAQMLNPAAWDGAAPAPTG
                     SVAALDGQLASDPEFYVGPPSLRRVGIQLPPDFRRIYPVTARGEGGSDNAVVVLQSTF
                     EPPQ"
     gene            901..4407
                     /locus_tag="PLS34_07780"
     CDS             901..4407
                     /locus_tag="PLS34_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HRP87405.1"
                     /translation="MKVETRRSAMLRRLGAGMAAFALSLLSVGPASANVSIANSPLFV
                     TSSADPNILFIIDDSGSMQWETMPDALTNIFSAGCCTNYMMWVYPRTAGLHGGGDYDG
                     IRVPSFATTSIMGHVYRSALVNTVYYNPGITYLPWANADGSRMPNASPTAAPNRPLFP
                     GYGTRNLTVNNTQAARWVENRTNADVNATRTFFPAVYSTYTGPAIADLNAAINDAASP
                     LWTIANYQRTEIIPANAPFTGEGRVNRSDCTAGSCTYDQEIQNFANWYSYHRNRIFAS
                     RAGIGSAFSVQPESMRVGLGAINTTGTVIRGVRPFAGTDRTAFFDDLYRQNIPPQGTP
                     LRLALDGAGQYFSRTDDLGPWSSTPGEAGGVDLECRQSYSILMTDGSASGTNTYAAAG
                     TRRDNNDGSGSNSMVINNPAQGGSDYTYTPTDPFQDGRSNTLADVAMYYWKRDLRPDL
                     PNLVPTTETNPAFWQHMVTFGVGLGVSGTIDPATAFAAIETGTPIAWPDPEYGTTNCT
                     GAACLARSDDVLHAAVNSRGGFFNAKDPEEFAIELASVLEDIVARVESSATSAATSSA
                     VLQTDTLLYTAGFRSGDWSGRLDARAVNEDGSLGGQVWDAEVQLALKTPAARNIVTRR
                     SDTGVAVTFQFANLSAAQQAALNRDPSGVNDGLGAQRVAWLRGDDNAHGSFRSRLPAG
                     GTAPNDLRLLGDIVNSNPKFQDGVLYVGANDGMLHAFNAETGEELFAYVPSPMLVPEG
                     SDTFAPVSRLMDPDYTHRYFVDGTVSVVDLSVAGTPRKVLVGSLGAGGRTVFALDVTN
                     PASFGPADVLWEFSHPELGYNVGPPGIVRLSDGTMAAVFGNGYNSTSHRAMLFAVNMN
                     TGALIQTVDTGVGSAGNPNGLASPVITDWPVSNLRANRVYAGDLQGNLWRFSVGGAPA
                     NWNDAGNVNMLFTAIGPSGSRQPITAQPIVALNPVNSNEVVVAFGTGSYFRTDDQDMP
                     GAAVQSLYGIIDTRTGASGVLRADLLEQEVTSQSVETFGGETVGVRIVSDNDFDASLH
                     DGWYLDLDVEDGERVISEGTYPSGPLQRRVRFSTLIPDENPCGTGRRGYIMDLDIISG
                     GRTRYSVFDLNRDGNYDDLDQLGGEAVSGVAWGQGERPTVLTPGDTSGDPPEFLYTGE
                     GEYIRGLGEEGIGGRQSWQQLR"
     gene            4433..4744
                     /locus_tag="PLS34_07785"
     CDS             4433..4744
                     /locus_tag="PLS34_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87406.1"
                     /translation="MRAGRLSVVLGAIVIATLVSGAIPDRAHAHVPEVAGLVQEWSPD
                     LRHLVLDGVEYALSPEFEVVDQDGRVLSAFQVSVGSRVRIVEHDGVVHTVVLLPSGTG
                     Q"
     gene            4741..5166
                     /locus_tag="PLS34_07790"
     CDS             4741..5166
                     /locus_tag="PLS34_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HRP87407.1"
                     /translation="MNIRNLRVQAAERGFTLIEVMIVVVVIAILASIAYPAYTEQVRQ
                     ARRADGTSALLNVSQRLERCFTQFNAYNNANCGVAFPQNSPEGNYEITAPVRTATQFT
                     LVATPIGPQAGDTRCTTFTLTQAGVQTATGTLGNDCWRR"
     gene            complement(5227..5661)
                     /locus_tag="PLS34_07795"
     CDS             complement(5227..5661)
                     /locus_tag="PLS34_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP87408.1"
                     /translation="MNRIPLLKTEMTPFPWWVDIDEPLLAARELMRQHKVRHLPVKDR
                     GKLVSVVTDRDLKFALDPDLGLPPRESLRVRDVCVYTAYIVDIECRLEEVLETMAERK
                     IGSALVTRDGKLVGIFTSPDACRVLARIIRERRGEDLDPQIA"
     gene            complement(5658..6314)
                     /locus_tag="PLS34_07800"
     CDS             complement(5658..6314)
                     /locus_tag="PLS34_07800"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRP87409.1"
                     /translation="MLRFARKELKHAPADRPDGSSLAETPWRRDGRVQRFANPFFTIY
                     EDEAWNRRAGRHFPYGIISFRNRAVGILPLEPDGRVWLVGQHRYATDEFSWELPMGGV
                     APDMSLLDGARKELKEETGLEAAEWEPLLQRVHLSNSVTDELGTVFLARGLREGAMAP
                     EASEELWVITTPLQDAVAAALDGRITDSLTVAGLLALAVRATEFGLDPGLFRAAGDGR
                     "
     gene            6524..>6749
                     /locus_tag="PLS34_07805"
     CDS             6524..>6749
                     /locus_tag="PLS34_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87410.1"
                     /translation="MKTTRWISFVLLAGVAALLQGCRDEAATAPVVELAPVGATRLEG
                     LGDHSKSVSSRHPEVQQWFDQGLMLTYGFNH"
BASE COUNT         1151 a         2303 c         2233 g         1062 t
ORIGIN      
        1 catcgcgccg ggcgccagtg gcgatctcgc cctctggcgg cgtgatgacg gcgtcgatac
       61 cgagctggtg gacggcgtcg aggacctgtg gctgcgctac ggcgaggatc tcaacaatga
      121 cggcgcgccc aacgtgtacg tcgaggcggc ggcggtcggc aactggagcc gcgtgcgcag
      181 cctgcgcatc agcctgctgc tggccagcaa cgaggacaac gtgaccgagg gcgcccagcc
      241 ggtggatttc cgcggcgccg tcgtcgtgcc cgccgacaat cgcttccgcc aggtgctgac
      301 gaccaccgtc ggcctgcgca accggcttcc ctgaggcaag agaatcatga aggcaaagca
      361 gccagagcaa tccctgaccc ggcaacgcga gcgcggctcc gcgctggcgg tggcgctgat
      421 tttcctgctg ctgatgacgc tgctgggcat atcggccatg cgcggcagca acatgcagga
      481 gcgcatggcg ggcaacctgc gcgatcgcaa cctggctttc caggcgtccg aagcggcact
      541 gcgcggcggc gagacctggc tgctgaacat cgtcaaccag cagacggccg acctcaatgc
      601 gcagatgctc aatccggcgg cctgggacgg cgcagcgccg gctccgaccg gcagcgtcgc
      661 cgcgctcgac ggacagctcg cgtccgaccc cgagttctat gtgggcccgc cgagcctgcg
      721 gcgcgtcggt atccagctgc cgcccgactt caggcgcatc tacccggtca cggcccgcgg
      781 tgagggcggt tcggacaacg ccgtcgtcgt gttgcagtcg accttcgagc cgccgcaatg
      841 accggcgtga ataccaagat tccagtcctg gcgccaacgg gcatgccgag gagtaccgag
      901 atgaaagtgg aaacgcgtcg atccgcaatg ttgcgccggc tgggtgcggg aatggcggcg
      961 ttcgccctgt cgctgctgtc cgtcggcccg gcgagcgcga acgtcagcat cgccaactcg
     1021 ccgttgttcg tgacctcgag cgccgatccc aacatcctgt tcatcatcga cgactccggc
     1081 tcgatgcaat gggaaacgat gcccgacgcg ctcaccaaca tcttcagcgc cgggtgctgc
     1141 acgaactaca tgatgtgggt gtacccgcgc acggcggggc tgcacggcgg gggtgactac
     1201 gacggcattc gcgtgccgtc ctttgcgacc accagcatca tgggccacgt ctaccgctcg
     1261 gcgctggtca acaccgttta ctacaatccg ggtatcactt acctgccgtg ggccaatgcc
     1321 gacggcagcc ggatgccgaa tgcctcgccc actgcggcgc cgaaccggcc gctgttcccg
     1381 ggctacggca cgcgcaacct gaccgtgaac aacacgcagg cggcccgctg ggtcgaaaac
     1441 cggaccaatg cggacgtcaa tgcgacgcgg acgttcttcc ccgccgtcta ttcgacctac
     1501 accgggccgg cgatcgccga cctgaacgcg gcgatcaacg atgccgccag tccgctttgg
     1561 accatcgcca attaccagcg cacggagatc atcccggcca acgcgccgtt cacgggcgag
     1621 ggccgcgtga accgcagcga ctgcaccgcc ggctcctgca cctatgacca ggaaatccag
     1681 aacttcgcga attggtacag ctatcatcgc aaccggatct ttgcgtcccg cgcgggcatc
     1741 ggcagcgcct tctcggtgca gccggaaagc atgcgcgtcg gcttaggcgc catcaacacg
     1801 acgggtaccg tgatccgcgg cgtgcgcccg ttcgccggca ccgaccggac ggcgttcttc
     1861 gacgaccttt accggcagaa cattccgccg cagggcacgc cgctgcgact ggcgctggac
     1921 ggcgccggcc agtacttctc acgcaccgac gatctcgggc catggagcag cacgccgggg
     1981 gaggccggcg gcgtcgatct cgagtgccgg cagagctact cgatcctgat gaccgacggc
     2041 tcggcctcgg gcaccaacac ctatgccgcg gccggcacac gccgcgacaa caacgacggc
     2101 tcgggctcca attccatggt aatcaacaac ccggcgcagg gcgggtccga ctacacctac
     2161 acgccgaccg atcctttcca ggacgggcgc agcaacaccc tggccgacgt ggccatgtat
     2221 tactggaagc gggacctcag gccggacctg ccgaacctgg tgccgaccac cgagacgaac
     2281 ccggcgttct ggcagcacat ggtgaccttc ggcgtgggcc tcggcgtgag cggcaccatc
     2341 gatcccgcga cggccttcgc ggccatcgag accggcacgc cgatcgcctg gccggatccc
     2401 gagtacggca ccaccaactg taccggcgcg gcctgcctgg cacgctccga cgacgtgctg
     2461 catgcggccg tgaattcgcg cggcggtttc ttcaacgcca aggacccgga ggagttcgcc
     2521 atcgagctgg cgagcgtact cgaggacatc gtcgcacgcg tcgaaagctc ggcgacctcc
     2581 gccgcgacca gctcggccgt gctgcagacc gacacgctgt tgtacaccgc gggcttccgc
     2641 agcggtgact ggtccggccg actcgacgcg cgggcggtca acgaggacgg cagcctcggc
     2701 gggcaggtgt gggacgccga ggtccagctc gccctcaaga cgcccgctgc ccggaacatc
     2761 gtgacccggc gcagcgatac cggcgtggcg gtgacgttcc agttcgccaa cctcagcgcg
     2821 gcccagcagg cggcgctcaa tcgcgatccg agcggcgtca acgacgggct cggtgcgcag
     2881 cgggtggcct ggctgcgcgg cgacgacaac gcgcatggct cgttccgcag ccggttgccg
     2941 gcgggcggca cggcgcccaa cgacctgcgt ctgctgggcg acatcgtcaa ctcgaacccg
     3001 aaattccagg acggcgtgct gtacgtcggc gccaatgacg gcatgctgca cgccttcaat
     3061 gcggaaacgg gcgaggaact gtttgcctac gtcccgagcc cgatgctcgt gcccgagggc
     3121 agcgatacct tcgcgccggt gagccggttg atggacccgg actacacgca ccgatacttc
     3181 gtcgacggca cggtgtcggt cgtcgacctg tcggtggccg gcacgccgag gaaagtcctg
     3241 gtgggcagcc tcggcgccgg cgggcgcacg gtgtttgcgc tcgacgtcac gaatcccgcg
     3301 agtttcgggc cggccgacgt gctgtgggaa ttctctcacc cggaactcgg ctacaacgtc
     3361 ggaccgccgg gaatcgtgcg gctcagcgac gggaccatgg ccgcggtatt cggcaatggc
     3421 tacaacagca ccagtcatcg cgccatgctg ttcgccgtga acatgaatac cggcgcgctg
     3481 atccagaccg tcgacaccgg tgtcggctca gcggggaatc cgaacgggct ggcttcgccg
     3541 gtaatcaccg actggccggt cagcaacctg cgcgccaatc gtgtttacgc cggcgacctg
     3601 cagggcaacc tgtggcgctt cagcgtcggc ggtgcgcccg ccaactggaa cgacgccggc
     3661 aacgtcaaca tgctgttcac ggccatcggc cccagcggca gccggcagcc catcacggcg
     3721 cagccgatcg ttgcgctcaa tccggtcaac agcaacgagg tggtggtggc cttcggtacc
     3781 ggcagctact tccgcacgga cgaccaggac atgcccggcg ccgcggtgca gagcctgtac
     3841 gggatcatcg acacgcggac cggcgccagc ggtgtgttgc gcgcggacct gctggagcag
     3901 gaggtcactt cgcagagcgt cgagactttc ggcggcgaaa cggtcggcgt gcgcatcgtg
     3961 tcggacaatg acttcgacgc gagcctgcat gacggctggt acctggacct ggacgtcgag
     4021 gatggcgagc gtgtgatcag cgagggcact tatccctcgg gaccgctgca gcgccgcgtc
     4081 cgcttcagca cactgatccc cgacgagaat ccctgcggca ccggtcgccg gggctacatc
     4141 atggatctcg acatcatctc gggcgggcgt acccgataca gcgtcttcga cctgaaccgg
     4201 gacggtaact acgacgacct ggaccagctc ggcggcgaag cggtatccgg cgtggcctgg
     4261 ggacagggcg agcgcccgac ggtattgacg ccgggtgaca cctcgggtga cccgccggag
     4321 ttcctctata ccggtgaagg cgagtacatc aggggtcttg gtgaagaggg catcggcggc
     4381 cgccagtcct ggcagcagct gagatgaggc cccgggaaca cggagcgaaa tcatgagagc
     4441 aggcagattg tcagtggtcc tcggtgcgat cgtgatcgcg accctggttt cgggcgcgat
     4501 tcccgaccgg gcccacgccc acgtccctga ggtggccggg ctggtgcagg agtggtcgcc
     4561 cgatctcagg cacctggtcc tggatggcgt cgagtatgcg ctgagcccgg aattcgaggt
     4621 cgtcgaccag gatggccggg tgctctcggc gttccaggtg tccgtcggct ccagggtacg
     4681 gattgtcgag cacgatgggg tcgtgcatac cgtcgtcttg ctgccgagtg gtacgggcca
     4741 atgaacatcc ggaatctgcg cgtgcaggct gccgaacgcg gcttcaccct catcgaagtc
     4801 atgatcgtcg tggtggtgat cgcgattctc gccagcattg cctatccggc ctacaccgag
     4861 caggtgcgcc aggcgaggcg ggcggacggc accagtgcgc tgctcaacgt gtcacagcgg
     4921 ctggaacggt gtttcacgca gttcaacgcc tacaacaatg ccaactgcgg ggtggccttc
     4981 ccccagaatt cgccggaagg caattacgag atcaccgcgc cggtccgcac ggccacccag
     5041 ttcacgctag tggccacacc gatcgggcca caggccggtg atacgcgctg caccaccttc
     5101 acgctgaccc aggcaggcgt gcagaccgcg accggcaccc tgggcaacga ctgctggcga
     5161 cgctgaattg gggacattcc ccaattaggt tgccaattgg gatgccaatt ggggaatgtc
     5221 cccaattcag gcgatctgcg ggtcgaggtc ctcgccgcgc cgctcgcgga tgatccgcgc
     5281 cagcacgcgg caggcgtccg gtgacgtgaa aatgcccacc agcttgccgt cacgggtcac
     5341 cagggccgag ccgatcttgc gctccgccat ggtctccagg acttcctcca ggcggcattc
     5401 gatgtccacg atgtacgcgg tgtagacgca gacgtcgcgc acccgcagcg actcgcgcgg
     5461 cggcaatccc aggtccgggt ccagtgcgaa cttcagatcc cggtccgtca ccacgctcac
     5521 cagcttgccg cggtccttca ccggcaggtg gcgcaccttg tgctggcgca tgagctcgcg
     5581 cgccgccagc aagggctcgt cgatgtccac ccaccacggg aacggcgtca tctccgtctt
     5641 gagcaggggg atgcggttca tcgaccgtct ccggcggctc ggaacaggcc cgggtccagg
     5701 ccgaactccg tggcgcgcac ggccagcgcc agcaatccgg cgaccgtcag cgagtcggtg
     5761 atgcggccgt ccagcgcggc ggccacggcg tcctggaggg gcgtcgtaat gacccacagc
     5821 tcctcgctcg cctcgggcgc catcgcgccc tcgcgaaggc cccgcgccag gaacaccgtg
     5881 ccgagctcgt cggtcaccga gttggacagg tgcacccgct ggagcagggg ctcccactcg
     5941 gcggcctcga ggccggtttc ttccttgagt tccttcctgg cgccgtccag caggctcatg
     6001 tccggcgcca cgccgcccat cggcagctcc caggaaaact cgtccgtggc atagcggtgc
     6061 tgcccgacca gccagacccg gccgtccggt tccagcggca ggatgcccac cgcccggttg
     6121 cgaaaggaaa tgatgccgta gggaaagtgg cggcccgcgc gccggttcca ggcttcgtct
     6181 tcgtagatcg tgaaaaacgg gttggcgaaa cgctggacgc gcccgtcccg tcgccatggt
     6241 gtctccgcca gcgatgagcc gtccggccgg tccgcgggcg cgtgtttcag ttccttgcgg
     6301 gcgaagcgaa gcatcggtct gtcgtcccgg acatgatcga acaaggggcg gcggaggccc
     6361 ctggcaaccg ccacccctat tatgccctga cgcccggcca tcggcgccag gttgaggccg
     6421 gccgccagcc ggcaaatgcc gcaacgcatg attgccagcc gggctgtaca ggtctattag
     6481 acaggcttgc ccttcacacg ccatggttgc tgggagcgtt gcgatgaaaa cgacccgctg
     6541 gatatcgttc gtgctgttgg ccggcgtcgc tgccctgctg caggggtgtc gggacgaggc
     6601 cgccacggca ccggtggtcg aactggcgcc ggtcggcgcc accaggctcg agggtctggg
     6661 cgaccacagc aagtcggtct ccagccgcca tcccgaggtg cagcaatggt tcgaccaggg
     6721 gctcatgctc acctacggct tcaaccatg
//
LOCUS       DAOMPG010000124         6717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4879_length_6717_cov_4.785350, whole genome shotgun sequence.
ACCESSION   DAOMPG010000124 DAOMPG010000000
VERSION     DAOMPG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_6717_cov_4.785350"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(861..1397)
                     /locus_tag="PLS34_07810"
     CDS             complement(861..1397)
                     /locus_tag="PLS34_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87411.1"
                     /translation="MNRAQLELVVSEVARRFDLDYFYVVGSAAVLAWIPDATAGVLTA
                     TRDVDVIPQPVEPERMSRVMDQIDFVLGEASDFDVEHGVYAQAVDDTTVAYAPVGWKD
                     RAIPLRVGRKTALCMDLHDLMLSKYGAGREKDLLFNRALAGTGTVFPRTLVERLQDVR
                     CDDRLRELIRARIAADFT"
     gene            complement(1394..1906)
                     /locus_tag="PLS34_07815"
     CDS             complement(1394..1906)
                     /locus_tag="PLS34_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87412.1"
                     /translation="MTRSPDNETLRNQIAALLASAPDGLSAPQIMSGLESRVSQPTLS
                     RRLMELRSQGRVAVVGKARATRYFYTGSRVEWAAVRSRLLHERIARRVARDPALLDRV
                     RKRLKQLSEVNPAGHVYHQQWSALLDADLPQLLRTMVAESEKSDALRKESPFTVLATR
                     KDRERVFAAQ"
     gene            complement(2152..2228)
                     /locus_tag="PLS34_07820"
     tRNA            complement(2152..2228)
                     /locus_tag="PLS34_07820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2192..2194),aa:Arg,seq:acg)
     gene            complement(2380..2473)
                     /locus_tag="PLS34_07825"
     tRNA            complement(2380..2473)
                     /locus_tag="PLS34_07825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Ser,seq:gct)
     gene            complement(2713..2931)
                     /gene="csrA"
                     /locus_tag="PLS34_07830"
     CDS             complement(2713..2931)
                     /gene="csrA"
                     /locus_tag="PLS34_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRP87413.1"
                     /translation="MLILTRRVGETVVIGDEVQVTVLGVKGNQVRLGVTAPRDVSVHR
                     QEIYERIRKEQAGEAGPAAGPDEGSADD"
     gene            complement(3083..4315)
                     /locus_tag="PLS34_07835"
     CDS             complement(3083..4315)
                     /locus_tag="PLS34_07835"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRP87414.1"
                     /translation="MARYIMKFGGSSVASVDHIRAVAAVIGRKRREGHQVAVVLSAMG
                     DTTDELLAKAHAISTRPSPRELDMLLSSGEQVSIALMAMALKELGLPARSWLGHQVRI
                     RTDAVHCKARIEEVETGRLEADLAAGVIPVVAGFQGIDADGQVTTLGRGGSDTTGVAL
                     AAALKADECQILTDVDGVYTTDPRMVPQARRLSRVTFEEMLELASLGSRVLQIRSVQF
                     AGKYNVPVRVLHNSGEGEGTLISYEEPDMEAPVVSGIAFSRDEAQVTITGVPDKPGNG
                     YAILNGVSEAHIVVDMIVMNAPRSGVVDFSFSVPRDEFEQAMELTRAAAAEIGAGDVL
                     GSPNVAKISVVGVGMRSHAGVATTMFEVLAREGINVRMVSTSEIKISVLIDEKYLELG
                     VRSLHEAFGLDREPHPAK"
     gene            complement(4333..>6717)
                     /gene="alaS"
                     /locus_tag="PLS34_07840"
     CDS             complement(4333..>6717)
                     /gene="alaS"
                     /locus_tag="PLS34_07840"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRP87415.1"
                     /translation="DLENVGYTARHHTFFEMLGNFSFGDYFKEDAIRYAWDYITGTLG
                     LPADKLWVTVYEQDDEAAAIWLEKIGVDPARFSRLGADSNFWAMGDTGPCGPCSEIFY
                     DHGPEVPGGPPGSPDEDGDRYVEIWNLVFMQFNRAADGSMTPLPKPSVDTGMGLERLA
                     AVLQGVHSNYDIDLFRALLSRAAELAGTDDHDNASLRVIADHIRACAFLVVDGVLPAN
                     EGRGYVLRRIIRRAIRHGYQLGIEEPFFWRLVETLEQQMGEAYPELAKARGHVEKVLH
                     QEEQRFAETLDQGMRLLDSAIAGLDGGVIPGETAFKLYDTYGFPLDLTADIARERGLA
                     VDHAGFERAMEAQRERARAASRFEADYGEKQDTELESKFVGYDSLGASGVILALYKDG
                     GRQVDALGEGDEGVIILDETPFYAESGGQVGDIGRIRAAAGEFQVTDTRKWGKAHAHH
                     GRMLRGRLEGGEQVRAEVDAGARQATVLNHSATHLLHAALRKVLGGHVQQKGSLVAPD
                     RLRFDFSHYEPLSRAQLAEIESLVNAQVRANAAAETRVVPYEEAVAGGALALFGEKYE
                     DRVRVLKLGDFSMELCGGTHVRRAGDIGLFKIVAESGIASGVRRIEAVTGEGALAWTA
                     RHEQSLQQIAALLKGGWDEAGQKVQQLIERARQLEKEVQQLQTKLATGGGSDDPAERA
                     ESVGDARIYIARLAGSDPKVLRDAVDRFKQKLGSGVVLLGAADGDKVRLVAGVTKDCV
                     ERISAVDLVNAAAQLVGGKGGGRPDLAQAGGADAARLDEALDMARAFARARLAG"
BASE COUNT         1113 a         2269 c         2157 g         1178 t
ORIGIN      
        1 gtcggcctcg gctcgaactc gctcttgctt gtcgggagtt cgactctctc ccgagccatc
       61 aggtcaaata aaaaagggcc ccgttgggac cctttttcat ttgactggcg cgcccggaga
      121 gattcgaact cccgaccttc tggttcgtag ccagagacag aaattagtat tttcagctac
      181 ttagctgcat tcttcccaaa ataactgctg catttcggcc ttggatgtca gcagcttggc
      241 gatagttttg ggaacggatg gcgaatccca ttcagcctcg gctgcgctaa ttacttgcgc
      301 gacagaatcc tccccaagca tcttccagca attgcggcgc ggtgtcacac gggaacaagg
      361 ttctccggcc tggcgaggag accctgcaag gcatctccgc caacccgatt tccacacggc
      421 gcagatcaag ttcgctccag ctattctcgg ccaggcagta cggcacagcc cgctcgaagt
      481 ccacgagcgt tccgacgacc gacctgtctc gcgtcggacc gacaccaacc ggctgcatcg
      541 cggcgacctc ccgctcgacc agcggatccg ccacatccag tcgccggagg cgttgcccga
      601 ccaagccggg tagccggtcc ggcagctggc ggacgtgtcg ggcaggctcg aggatagcaa
      661 gcagcgaacg cgaggacacg agcagtaaca ggggctgccg atcgacaatg aaccgattca
      721 cataccagtc gccgagcgcc gtgtcagcgc tgtgctcttc tactgagacc ggcggaagcc
      781 acctcaggac tttcttcgtc gccctgagca tcaccatccc ggaacgaccc gcagacctga
      841 acgtcgggta ctggagacga tcacgtgaaa tctgccgcga tccgggcccg gatcaactcg
      901 cgcaggcgat catcacagcg cacatcctgg agacgctcaa ccaaggttct ggggaatacg
      961 gtaccggtcc ctgccaacgc ccgattgaag agcagatcct tctcgcgccc tgcaccgtac
     1021 tttgagagca tcaagtcatg aaggtccatg catagcgccg tctttcggcc aacccgtaac
     1081 gggatggcgc ggtccttcca tcccaccggc gcataggcca ccgtcgtatc atcgacggcc
     1141 tgtgcgtaca ctccatgttc gacgtcaaaa tccgatgcct cgcccaggac gaaatcgatc
     1201 tggtccatga ctctggacat gcgctccggc tcgacgggtt gcgggatcac gtcgacgtcg
     1261 cgggtcgccg tcaggactcc cgctgtggcg tcaggaatcc aggccagcac agcggcagac
     1321 ccaacgacgt aaaagtaatc caggtcaaac cgacgggcga cctccgaaac gaccagttcg
     1381 agttgggcgc ggttcattgc gcggcaaata cccgctcgcg atccttgcgc gtggcgagca
     1441 cggtaaacgg ggattctttc ctcaacgcat ccgacttttc ggactcggcg accatggttc
     1501 ggagcaattg cggcaggtcc gcatccagca aggcgctcca ctgctggtga taaacgtgcc
     1561 cagcggggtt tacctcggac aactgtttca gtcgtttcct gactcgatcc aagagggcgg
     1621 gatcacgagc gacgcggcgc gcaatcctct cgtgcagcaa gcggctgcga acggcagccc
     1681 attccactct gctgccagtg tagaaatagc gcgtcgcgcg agccttgccg accacggcca
     1741 cgcgaccctg cgagcgaagt tccattaatc gtcgggacaa agtcggctga ctgacccgac
     1801 tctcaaggcc cgacatgatc tgcggcgcgc tcaggccgtc cggggcgctc gccaacagcg
     1861 ccgcgatctg gtttctgagg gtttcattat cgggtgatct cgtcatggga cggaacgagt
     1921 gtgaatgaat tgtgaataga tactaacaga ctcgacgggg cgggaacagt tcgcggctgg
     1981 cgcgcccgga gagatgaccg gcgccgctgc gcggcaccgt tgtctcggct cgaactgggc
     2041 ttgtctgtcg ggagtctgag ccccccgaga cattggtcaa acaaaaaaat cggggtcaga
     2101 caccagtttc ccgtttgcgg gaaactggtg tctgaccccg atttttttga ctggcgcgcc
     2161 cggagagatt cgaactcccg accttctggt tcgtagccag acactctatc caactgagct
     2221 acgggcgcgt atttctgaaa actgcaaact gtggcggaga gggagggatt accggccgcc
     2281 tgcggcggct cgttccctgc gccggctccg ccggctccgg gtcgaacccg gtctttgttg
     2341 cttcgagggt tcgaatccct cgtgatttaa cactaactgt ggcggagagg gagggattcg
     2401 aaccctcgaa ggggctttat gaccccttac tcccttagca ggggagcgct ttcgaccact
     2461 cagccacctc tcctgtcaaa atcctccggt cggcgaggct cccgccgaaa caacccacgg
     2521 tcgggattac tcgtcgctgc gctcctcgcc ctgcgggccg ccgtcgcttc gcgacgacgt
     2581 tccctcgccc cgctgcgcgg gtctcgggtc gaaccctcga aggggcttta tgacccctta
     2641 ctcccttagc agggcagcgc tcccgccgaa aggcgaggaa tgatagcggg gggtggtggg
     2701 gaggtaaagg gctcagtcgt ccgcgctgcc ctcatcgggt ccggccgcgg ggccagcctc
     2761 gccggcctgc tccttgcgga tcctctcgta gatctcctgg cggtggaccg agacgtcgcg
     2821 cggcgccgtg acccccaggc ggacctggtt gcccttcacg ccgagcacgg tcacctggac
     2881 ctcatcgcca atgacgaccg tctcgccaac acgtctcgta aggatcagca tttctacttc
     2941 cctgcccgct gccgaaaccg gattctggac caatgttaga catttcgcag cggtcgcaca
     3001 ccaagacata agtcaagggt atgaaaaacg gacgattttc ccacatacgg tgatcgtggc
     3061 gcgttctgtg ggatgttatg acctattttg cgggatgagg ttcccggtcc aacccgaagg
     3121 cctcgtgcag gctgcgcact ccaagctcca ggtacttctc gtcgatgagc accgagatct
     3181 tgatctcgga ggtcgagacc atgcgcacgt tgatgccctc gcgcgccagc acctcgaaca
     3241 tggtggtggc gacgccggcg tggctgcgca tgcccacgcc gaccacggaa atcttcgcca
     3301 cgttggggct gccgagcacg tcgccggcgc cgatctccgc ggccgccgcc cgggtcagct
     3361 ccatggcctg ctcgaactcg tcgcgcggca cgctgaaact gaagtccacc acgccggagc
     3421 gcggcgcgtt catgacgatc atgtcgacga cgatgtgcgc ctcggacacg ccgttgagaa
     3481 tcgcgtagcc gttgcctggc ttgtcgggca cgccggtgat ggtcacctgg gcctcgtccc
     3541 tggagaaggc gatgccggag acgacgggtg cttccatgtc gggttcctcg tagctgatca
     3601 gggtcccctc gccctcgccg ctgttgtgca gcacgcggac gggaacgttg tacttgccgg
     3661 cgaactgcac cgagcggatc tgcagcacgc gcgagccgag gctggcgagc tcgagcattt
     3721 cctcgaaggt gacgcgggac aggcgacggg cctgcggcac catgcgcggg tccgtcgtgt
     3781 acacgccatc gacgtcggtg agaatctggc actcgtcggc cttgagcgcc gcggccagcg
     3841 ccacgccggt ggtgtccgag ccgccgcgcc cgagtgtcgt cacctggccg tccgcgtcga
     3901 tgccctggaa gccggccacg acggggatga cgccggccgc aaggtcggcc tcgagccgcc
     3961 cggtctcgac ttcctcgatg cgcgccttgc aatgcacggc gtcggtgcgg atgcgcacct
     4021 ggtggcccag ccaggagcgc gccggcaggc cgagttcctt cagcgccatc gccatcagcg
     4081 cgatcgacac ctgctcgccg gaggacagca gcatgtcgag ctcgcgcggg ctgggccggg
     4141 tggagatcgc gtgggccttg gcgagcaact cgtcggtggt gtcgcccatg gccgacagga
     4201 ccacggccac ctggtggccc tcccgccgct tcctgccgat caccgccgcg acggcgcgga
     4261 tgtggtctac gctggcgacg gagctgccgc cgaacttcat gatgtagcgg gccattgctc
     4321 gcgccttcgt ggtcagccgg ccaggcgcgc gcgcgcgaag gcgcgggcca tgtccagcgc
     4381 ctcgtccagc cgcgccgcgt ccgcgccgcc ggcctgcgcg aggtccggcc ggccgccacc
     4441 cttgccgccg accagctggg ccgccgcgtt gaccaggtcc accgcgctga tgcgctcgac
     4501 gcagtccttg gtcacgccgg ccaccagccg caccttgtcg ccgtcagcgg cgccgagcag
     4561 caccacgccg ctgccgagct tctgcttgaa gcggtccacg gcgtcgcgca gcaccttggg
     4621 gtcactgccg gcaagccgcg cgatgtagat gcgcgcgtcg ccgaccgact cggcacgctc
     4681 cgcgggatca tcactgccgc cgccggtggc cagcttcgtc tgcagctgct ggacttcctt
     4741 ctcgagctgc cgcgcgcgtt cgatgagctg ctgcaccttc tggccggcct cgtcccagcc
     4801 gcccttcagc agggcagcaa tttgctgcag gctctgttcg tggcgcgccg tccacgccag
     4861 cgcgccctcg ccggtgaccg cctcgatgcg gcgcaccccg gaggcgatgc cgctctcggc
     4921 gacgatcttg aacagcccga tgtcgccggc ccggcgcaca tgcgtgccac cgcaaagctc
     4981 catcgagaaa tcgcccagct tgaggacgcg cacgcgatcc tcgtacttct caccgaacag
     5041 cgccagcgcc ccgccggcca ccgcctcctc gtaagggacc accctggtct cggccgcggc
     5101 attggcgcgc acctgggcgt tcaccagcga ctcgatctcg gcgagctgcg cgcggctgag
     5161 cggctcgtag tgcgagaagt cgaagcgcag ccggtcgggt gcgaccagcg agcccttctg
     5221 ctgcacgtgc ccgccgagca ccttgcgcaa ggcggcgtgc agcaggtgcg tcgcggagtg
     5281 gttgagcacc gtggcctggc gcgcaccggc atccacttcg gcacgcacct gttcgccgcc
     5341 ttcgaggcgg ccgcgcagca tgcggccgtg gtgggcatgc gccttgcccc acttgcgggt
     5401 atcggtgacc tggaactcgc cggccgcggc gcgaatccgg ccgatatcgc cgacctggcc
     5461 gcccgactcg gcgtagaagg gcgtctcgtc gaggatgatg acgccctcgt cgccctcgcc
     5521 gagtgcatcc acctggcggc cgccgtcctt gtacagcgcg aggatcacgc ccgaggcgcc
     5581 gagcgagtcg taaccgacga acttggactc gagttcggtg tcctgcttct caccgtagtc
     5641 cgcctcgaag cggctggcgg cgcgggcgcg ctcgcgctgc gcctccatgg cgcgttcgaa
     5701 gccggcgtgg tccaccgcca ggccgcgctc gcgcgcgatg tcggcggtca aatccagcgg
     5761 gaagccgtag gtgtcgtaga gcttgaaggc ggtctcgccg gggatgacgc cgccgtcgag
     5821 gccggcgatg gccgagtcga gcagccgcat gccctggtcg agcgtctcgg cgaagcgctg
     5881 ctcctcctgg tgcagcactt tctcgacgtg gccgcgcgct ttcgcgagtt cggggtaggc
     5941 ctcgcccatc tgctgctcga gcgtctcgac cagccgccag aagaacggct cctcgatgcc
     6001 gagctggtag ccgtggcgga tggcgcggcg gatgatgcgc cggagcacgt agccgcggcc
     6061 ctcgttggcg ggcagcacgc cgtccaccac caggaaggca caggcgcgga tatggtccgc
     6121 gatcacccgg agcgaggcgt tgtcgtgatc gtccgtgccg gccagctcgg cggcgcggct
     6181 gagcagcgcg cggaacaggt cgatgtcgta gttggagtgc acgccctgca gcacggcggc
     6241 gagccgctcg aggcccatgc cggtgtccac cgagggcttg ggcagcggcg tcatcgagcc
     6301 gtcggccgcg cggttgaact gcatgaacac caggttccag atctcgacgt agcgatcgcc
     6361 gtcctcgtcg ggcgagccgg gcggcccgcc gggcacctcg ggaccgtggt cgtagaagat
     6421 ctcggagcag ggcccgcagg ggccggtatc gcccatggcc cagaagttgg agtcggcgcc
     6481 gagccggctg aagcgtgccg ggtccacgcc gattttttcc agccagatgg cggcggcctc
     6541 gtcatcctgc tcgtagaccg tcacccagag cttgtcggcc ggcagcccga gcgtgccggt
     6601 gatgtagtcc caggcgtagc ggatggcgtc ttccttgaag taatcgccga agctgaagtt
     6661 gcccagcatc tcgaagaagg tgtgatgccg ggcggtgtag ccgacgttct cgaggtc
//
LOCUS       DAOMPG010000125         6660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_4932_length_6660_cov_4.359425, whole genome shotgun sequence.
ACCESSION   DAOMPG010000125 DAOMPG010000000
VERSION     DAOMPG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_6660_cov_4.359425"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLS34_07845"
     CDS             <1..490
                     /locus_tag="PLS34_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRP87416.1"
                     /translation="VGDVRVINLYVPNGQAVGTDKYAYKLAWLDTLRLHLAAELARYP
                     RMVVMGDFNIAPDDRDVHDPAEWAGKVLCSEPERAALARVLELGFVDTFRLFEQPEGV
                     YSWWDYRAAGFRRNRGLRIDLVLASKALAGACSRSWVDIEPRRNERPSDHAPAVAVFD
                     VA"
     gene            575..1612
                     /locus_tag="PLS34_07850"
     CDS             575..1612
                     /locus_tag="PLS34_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87417.1"
                     /translation="MTATATKGRVIMLRDRRSDHDVTNTVPLTHWEPVWQVVHDSFKR
                     LYPGIPLRVLRQGFEDFDALFRGRMPGYLGCDTVYHDIQHTLDMTLALARLIQGHETS
                     VAAEERLGAPRAILGIISSLFHDSGYIRHRDDEQHANGAEFTRSHVSRSAQFLDRYLN
                     QVGLGAASGIARQIVHFTGYEVRLDQIRVVAEKDRKLGHLMGTADLLAQMADRCYLEK
                     CRDRLYPEFVLGGIAVWRDPSGRVITRYRSGLDLLRQTPAFFRQVCEPRLETSFEGAY
                     RYMDEVFEGRNPYMVAIERNMFYLERVLEGERWPLLRRRPSCELAETDALERIRRMVA
                     ERLKEYRAEAA"
     gene            complement(1647..2714)
                     /locus_tag="PLS34_07855"
     CDS             complement(1647..2714)
                     /locus_tag="PLS34_07855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRP87418.1"
                     /translation="MRVSLVMTSPGMGGIQQSVVPYALALKAGGHAVQIVLRHGADIL
                     GEALERGLAEDTWVLAGGWRALRPWQTRELRRRLLAFGPDIVVGFAQKGLFEAERAMR
                     GRDVPVVTRVGTMRAKRQRRFFKADGWLATTPEMQRALAGLGLPAERIFVVPNFLADP
                     AAPRRLTAPGDVALAGALGRFARRKGFEVLIEAMALLAQRGIAARCDIAGDGRHRNAL
                     ARLIDARGVGDAVRLVGWLGNEQKAAFLAGLDLFVCPSLDEPFGFVYLEAMRLGIPVV
                     TTPTVGAGYIFSDGAGAQFVPFGDAAALAATIERLVQDPALRRTLGDQAAAIYEARFD
                     LAAGERNLGTALAALRQAGRR"
     gene            2823..3608
                     /locus_tag="PLS34_07860"
     CDS             2823..3608
                     /locus_tag="PLS34_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRP87419.1"
                     /translation="MPRRVETFSKRLFGALGLEVRRKSRFAPPLNFFETILEGLIAGR
                     DMLNVVQVGANDGAHNDPIHAFLMAHRQHTNALLIEPQPELIPHLEAAYAEHPRVAIY
                     NGAIGATDEFVLHRIRPALWGEFRSSYMKGAPAYRAPSGLTSADRAHVLAAARKYLPA
                     GIDAEDAVEAFAVPCRRLSQLLAETGFPTPIQLLQVDAEGADDEVLRACDVPGLRPAV
                     INYESRHLPAARREALEVLLTGEGYRLFHWHKSDTLAVRSQRT"
     gene            3688..5355
                     /locus_tag="PLS34_07865"
     CDS             3688..5355
                     /locus_tag="PLS34_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HRP87420.1"
                     /translation="MKIRHLDLFRQSLSSTGLLVGTLLFAASLSPSLVPRPFMLQALL
                     AGVALAAGYGVGVFLLWLWHFMELPEFKGRVLWIGKIVAWTICTAVAVGFLVRASAWQ
                     DSIRELMGLPPVETTRPFSVGLIAVLVFALLLGLARLLRFIKRTVARTADLWVPGRIA
                     LVVGVLVTATLVWSLATGLFSRLVLSSFDASFQQLDLLIEDELEPPGHPLATGSGHSL
                     LNWDGLGRQGRRFIARASTLEELEQIGPAEIRRPLRIYVGLNSAETIEERVRLALGEM
                     QRVGAFQRDYLVIVTPTGTGWVDPGAIRSLEHLVGGNVASVAVQYSYLPSWLSLLAQA
                     EYGQETARALFAEVYRHWRNLPAERRPRLYLHGLSLGALNSARSTDTWDLVGDPIAGA
                     LWSGPPFRSETWQWLTARRNPGTPAWLPRFRDGSVVRFANQHGGLEDFDAPWGPFRIA
                     YLQYASDPISFFRPNALYREPAWMQGERAHDVSPKLRWYPIVTSLQLAVDIAAAETAP
                     IGHGHNYAAEHYIEAWHALLKPPGWDAGRLGELKRRIGDLSPDPAGH"
     gene            complement(5695..>6660)
                     /gene="glmS"
                     /locus_tag="PLS34_07870"
     CDS             complement(5695..>6660)
                     /gene="glmS"
                     /locus_tag="PLS34_07870"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRP87421.1"
                     /translation="LFDATRAVQIVACGTSYHAGLVARYFIEKLARIPCMVEIASEYR
                     YRQPVVPEGTLFVTISQSGETLDTLEALRAAKKMGYVGTLGICNVPESALVRESDLVL
                     MTRAGPEIGVASTKAFTTQLAALSLLGLVLARRHGANGVEADLVRQLAAVPALLEKIL
                     EMDHDIELLAERFVDKHHALFLGRGIQFPVALEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDADMPVIVVAPNNELVEKLKANMQEVRARGGELFVFADPDAGIANGPGVTVLKM
                     PVHVEDFQAPIAFTVPLQLLAYHVAVLRGTDVDQPRNLAKSVTVE"
BASE COUNT          999 a         2367 c         2216 g         1078 t
ORIGIN      
        1 ggtcggcgac gtgcgcgtga tcaacctgta cgtccccaac ggccaggcgg tcggtaccga
       61 caagtacgcc tacaagctgg cgtggctgga caccctgcgc ctgcacctgg ccgccgagct
      121 ggcgcgatat ccgcgcatgg tcgtgatggg cgacttcaac atcgcgccgg acgatcgcga
      181 cgtgcacgac ccggccgagt gggccggcaa ggtgctgtgc agcgagcccg agcgggccgc
      241 gctggcccgc gtgctggagc tcggtttcgt cgacaccttc cgcctgttcg aacagccgga
      301 gggcgtgtac agctggtggg actaccgcgc cgccggcttc cggcgcaacc gcggcctcag
      361 gatcgacctg gtgctggcca gcaaggcgct ggccggagcc tgcagccgct cctgggtcga
      421 catcgagccg cgccgcaacg agcggccgtc ggaccatgcg ccggccgtgg cagtgttcga
      481 cgtcgcttga cgggcgcggc ggtccctgtg actgtggtca cataaccgcc acgccgacat
      541 ggcgccacgc gccctgcttt tataaactca ccagatgacc gccaccgcca ccaagggccg
      601 cgtgatcatg ctccgggacc ggcgcagcga ccacgacgtg accaacaccg tgccgctgac
      661 ccactgggag ccggtgtggc aggtggtcca cgacagcttc aagcgcctgt accccggcat
      721 ccccctgcgg gtcctgcgcc agggcttcga ggacttcgac gcgctgttcc gcggccgcat
      781 gcccggctac ctgggctgcg acaccgtcta ccacgacatc cagcacacgc tcgacatgac
      841 gctggcgctg gcgcgcctga tccagggcca cgagacctcg gtggcggctg aggagcggct
      901 cggggcgccg cgcgccatcc tcggcatcat cagctcgctg ttccacgact caggctacat
      961 ccggcaccgg gacgacgagc agcatgccaa cggcgccgag ttcacgcgct ctcacgtcag
     1021 tcgcagcgcc cagttcctgg accgctacct gaaccaggtc ggcctcggcg ccgcctccgg
     1081 gatcgcgcgc cagatcgtgc acttcaccgg ctacgaggtg cgcctcgacc agatccgcgt
     1141 ggtcgccgag aaggaccgca agctcggcca cctgatgggc accgccgacc tgctcgcgca
     1201 gatggcggac cgctgctacc tggagaagtg ccgcgaccgg ctgtacccgg agttcgtgct
     1261 cggcggcatc gccgtgtggc gcgacccgtc cggtcgggtg atcacgcgct atcgttccgg
     1321 tctcgacctg ctgcgccaga ccccggcgtt cttccgccag gtgtgcgagc cgcgcctgga
     1381 gacctccttc gagggcgcct accgctacat ggacgaggtg ttcgagggcc gcaaccccta
     1441 catggtggcg atcgagcgca acatgttcta cctggaacgc gtgctggagg gcgagcgctg
     1501 gccgttgctg cgtcgccgcc cgtcctgcga gctcgccgag accgacgcgc tcgagcgcat
     1561 ccgccgcatg gtcgcggagc ggctcaagga atatcgcgcc gaggcagcct gagcgcggcc
     1621 cgtgccttgc gggccaaaga tacggcctat cgtctccctg cctgccgcag cgccgccagc
     1681 gccgtaccga gattgcgctc acccgccgcc aggtcgaagc gcgcctcgta gatcgccgcg
     1741 gcctggtcgc cgagcgtgcg ccgcagcgcc gggtcttgta ccaggcgctc gatggtcgcc
     1801 gccagcgcgg ccgcatcgcc gaacggcacg aactgcgccc ctgcgccgtc gctgaagatg
     1861 tagccggcgc cgaccgtcgg cgttgtgacc acgggaatcc cgaggcgcat cgcttccagg
     1921 tagacgaagc cgaaaggctc gtccagcgag gggcagacga acaggtcgag gccggccagg
     1981 aaggccgcct tctgttcgtt accgagccag cccacgagcc ggacggcatc gccgacaccg
     2041 cgcgcatcga tcagtcgcgc cagcgcattg cgatgtcggc cgtcgccggc gatgtcgcag
     2101 cgggcggcga tgccccgctg cgcgagcagg gccatggcct cgatgagcac ctcgaagccc
     2161 ttgcgccggg cgaaacgccc cagggcgccg gccagggcga cgtcccccgg cgcggtgagg
     2221 cggcgcggtg cggccggatc cgccaggaag ttcggcacca cgaagatgcg ctccgccggc
     2281 aggcccaggc ccgccagcgc ccgctgcatt tccggcgtcg tggccagcca cccgtcggcc
     2341 ttgaaaaagc gccgctggcg cttggcccgc atggtaccga cgcgcgtgac caccgggacg
     2401 tcccgcccgc gcatggcgcg ctcggcctcg aacaacccct tctgcgcgaa gcccacgacg
     2461 atgtccggcc cgaacgccag cagcctgcgc cgtagttcgc gcgtctgcca cggccgcagg
     2521 gcgcgccagc cgccggccag cacccaggtg tcctcggcca ggccgcgctc cagcgcttcg
     2581 ccgaggatgt ctgcgccgtg ccgcaacacg atctgcaccg cgtggccgcc ggccttcagc
     2641 gccagcgcat acggcaccac ggactgctgg atgccgccca tgcccggcga ggtcatcacc
     2701 agcgaaaccc tcactgcgcc gcccctggcc ggcgcggcaa cggctgccgc cgcgaccggc
     2761 tccccgccgg ccgggtttcg ctacactgcc ggtcgcagcc ccctgttccg gagtcggccc
     2821 gcatgccgcg acgcgtggag acattcagca agcgactgtt cggcgcgctg ggcctcgagg
     2881 tccgccgcaa gtccaggttc gcgccgccgc tgaatttttt cgaaaccatt ctggagggac
     2941 tcattgccgg ccgggacatg ctcaacgtgg tgcaggtcgg cgccaacgac ggcgcgcaca
     3001 acgaccccat ccacgctttc ctgatggcac accgacagca taccaatgcg ctgctcatcg
     3061 agccgcagcc ggagctcatc ccgcatctcg aggcggcgta cgccgaacat ccgcgagtcg
     3121 cgatctacaa cggcgccatc ggcgccacgg acgagttcgt cctgcatcgc atccggccgg
     3181 cgctgtgggg cgagttccgg tcttcctaca tgaagggcgc gcccgcctac cgcgcgccga
     3241 gcggcctgac ctcggccgac cgggcgcacg tgctggccgc cgcccgcaag tacctgcccg
     3301 ccgggatcga tgccgaggac gccgtggagg ccttcgccgt gccctgccgc cgcctgtcgc
     3361 agctgctggc ggagaccggc ttcccgaccc cgatccaact gctgcaggtg gacgcggagg
     3421 gcgccgacga cgaggtgctc cgcgcctgcg acgtgccggg gcttaggccg gcagtcatca
     3481 actacgaatc aaggcacctg ccggcagcac ggcgcgaggc gctggaggtt ctgctcaccg
     3541 gcgagggcta ccggttgttc cactggcaca agtcggacac gctggccgtg cgctcgcagc
     3601 gcacttagcc gtcactgccg ccgcgtgccg ccaaccccgg ccagcgttcc gcgcacgccc
     3661 ccgggccatc cgtgcgaaga ttaaggcgtg aagatcagac acttggatct gttccgccag
     3721 tcgctgtcga gcaccgggct gctggtcggc accctcctgt tcgccgcttc gctatcaccc
     3781 agcctggtcc cgcgtccgtt catgttgcag gccctcctgg cgggcgtggc gcttgccgcc
     3841 ggctacgggg ttggcgtgtt cctgctctgg ctgtggcatt tcatggagct gccggagttc
     3901 aaggggcgcg tactatggat cggcaagatt gtcgcctgga cgatctgcac tgccgtggcc
     3961 gtaggcttcc tggtgcgcgc ttcggcctgg caggacagca tccgtgaact catggggcta
     4021 ccgccggtcg agacgacgcg acctttttcg gttggcttga ttgctgtcct ggtgttcgcg
     4081 ctgctgctgg gcctggcgcg gctgctccgc ttcatcaagc gcaccgtcgc ccgaaccgcc
     4141 gatctctggg tgcccggccg gatcgccctg gtggtcggtg tcctggtcac ggcgacactg
     4201 gtctggagcc tggcgacggg tttgttttca cggctggtgc tgagcagctt cgacgcatct
     4261 tttcagcagc tggacctgct catcgaggac gaactcgagc cgcccgggca tccgctggcg
     4321 acggggtcgg gccattcgct cctcaactgg gacggcctcg gcaggcaggg acggcgcttc
     4381 atcgcccgtg catcgaccct cgaggagctg gagcagatcg gtccggcaga aatccgcagg
     4441 cccctgcgaa tctacgttgg gctgaattcg gccgaaacca tcgaggagcg agtccggctg
     4501 gcgctgggcg aaatgcagcg cgtgggcgcc ttccagcgcg actacctggt catcgtgacg
     4561 cccaccggca cgggctgggt cgaccccggt gccatccgca gtctcgagca cctggtcggc
     4621 ggcaacgtgg ccagcgtcgc cgtgcagtat tcttacctgc cgagctggct gtcgttgctg
     4681 gcccaggccg agtacggcca ggaaacagcg cgcgcattgt ttgccgaagt ctaccggcac
     4741 tggcgcaacc tccctgccga gcgccgcccg cgcctgtact tgcacggctt gagcctgggc
     4801 gcgctgaact cggcgcgctc gactgacacc tgggacctcg tcggcgaccc catcgctggc
     4861 gccttgtgga gcggaccacc ctttcgtagc gagacctggc agtggctgac cgcccggcgt
     4921 aaccccggca caccggcctg gctgccccgc ttccgcgacg gctcggtggt ccggttcgcc
     4981 aaccagcatg gcgggctgga agacttcgac gcgccgtggg gtcccttccg catcgcctac
     5041 ctgcaatatg ccagcgaccc gatcagcttt ttccggccga atgccttgta tcgcgaaccg
     5101 gcctggatgc agggggagcg agcgcatgac gtctcgccga agttgcgttg gtacccgatc
     5161 gtgacttccc tgcagctagc ggtggatatt gctgcggccg agaccgcgcc gatcggccac
     5221 ggccacaatt atgcggccga gcactacatc gaggcatggc acgcactgct caagccgccg
     5281 ggctgggacg ccgggcggct gggcgagctg aaacgccgga tcggcgatct gtcgcccgac
     5341 cccgcagggc actgatcggg tttcacccca tttcgacgaa tggttgagtc aggagtagtg
     5401 ggttctgctc agtcgtttcc atctggcgcc gacgaacagg attagtccct gttcaagata
     5461 caaaggaccc ggtttccctt cgtatggcgt gttgtttgtt tggactgtaa gcaggcctga
     5521 ctgggccgac ccggatcgcc caactcgacc cgtgtcgcac tgaatttagc ccgatggggc
     5581 cgggcccctg ctgtgctgca catccggccg ctttttctca ggcacgaact tcggccggcc
     5641 ggaaaaggta aggacattat tgtttgctga cagattcgtg atctggcctg cgctttactc
     5701 caccgtaacc gacttcgcca gattgcgcgg ctggtccacg tcggtgccgc gcagcacggc
     5761 gacgtgatag gcgagcagct gcagcggcac ggtgaaggcg atcggcgcct ggaagtcctc
     5821 tacgtgcacc ggcatcttca gcacggtcac gccggggccg ttggcgatgc cggcgtccgg
     5881 gtcggcgaac acgaacagct cgccgccgcg ggcgcgcact tcctgcatgt tggccttcag
     5941 cttctcgacc agctcgttgt tgggcgccac cacgatcacc ggcatgtccg cgtccaccag
     6001 cgccagcggg ccgtgcttca gctcgccggc cgggtaggcc tcggcgtgga tgtatgagat
     6061 ttccttcagc ttcagcgcgc cttccagcgc caccggaaac tggatgccgc ggccgaggaa
     6121 cagtgcgtgg tgcttgtcca cgaagcgctc ggcgagcagc tcgatgtcgt ggtccatctc
     6181 gaggatcttc tccagcagcg ccggcacggc ggccagctgc cgcaccaggt ctgcctcgac
     6241 gccgttcgcg ccgtggcggc gcgccagcac caggccgagc agcgacagcg cggcgagctg
     6301 ggtggtgaag gccttggtgg aggccacgcc gatctccgga ccggcgcggg tcatcagcac
     6361 caggtcggac tcgcgcacca gcgcgctctc cggcacgttg cagatgccca gcgtgccgac
     6421 gtagcccatc ttcttcgcgg cgcgcagcgc ctcgagggta tccagcgtct cgccggactg
     6481 ggagatggtg acgaacagcg tgccctcggg caccaccggc tggcggtagc gatactcgct
     6541 ggcgatctcc accatgcagg ggatgcgcgc cagcttctcg atgaaatagc gcgccaccag
     6601 gccggcatgg tagctggtgc cgcaggcgac gatctgcacg gcgcgcgtgg cgtcgaacag
//
LOCUS       DAOMPG010000126         6539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5045_length_6539_cov_3.621067, whole genome shotgun sequence.
ACCESSION   DAOMPG010000126 DAOMPG010000000
VERSION     DAOMPG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_6539_cov_3.621067"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLS34_07875"
     CDS             <1..417
                     /locus_tag="PLS34_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87422.1"
                     /translation="EPAAPVAAPVAAQPETAAPAADTVAGGIPLEVTLDPALAARIPG
                     PTPMFIIARNPGGGAPLAVIRSNSTELPMSVTLSDANAMMAGVTISDQAELELIVRVA
                     LSGSPAQRPGDLYGAVNYVRGSGEAPTRIRIDSVAE"
     gene            436..696
                     /locus_tag="PLS34_07880"
     CDS             436..696
                     /locus_tag="PLS34_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HRP87423.1"
                     /translation="MADDLKARFEQASVDVKSLSQRPSDNDMLSLYALYKQATEGDVA
                     GDKPGFFDFVARAKYEAWEGLKGTGAAEAMQKYIDKVRSLGA"
     gene            702..1571
                     /locus_tag="PLS34_07885"
     CDS             702..1571
                     /locus_tag="PLS34_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRP87424.1"
                     /translation="MSSRLSFPVQPSIAAAYLRAMFDRRPRRLPTDAGVIRVEAEAGG
                     VVADPARLAAYRDVCGLPDDGMLPLTYPHILAGGVHMAMLLHRDFPVRMVGLVHLSND
                     IELFTPIPAGARLALDCTLESGRSKPRGDEFRMVTEALWNGQLAWRETMSFLAPAPRQ
                     RSRAPGEAPRFPPLLGQWPVPADTGRRYARVSGDWNPIHLASLLARPFGFPAAIAHGM
                     WTVARCLAWLGEGTPGPGARLEVRFLKPLLLPGQVGLHVESPMESGDREFWLLSTDGA
                     TPHLKGLWNPGTG"
     gene            complement(1603..3036)
                     /locus_tag="PLS34_07890"
     CDS             complement(1603..3036)
                     /locus_tag="PLS34_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRP87425.1"
                     /translation="MRDTRRAARRFLTAVEFDDLGALRRVSVCLLVAAVVAFLPDWVG
                     LDAAGRRCLFILVLAAGLWATEAIPAFAVAFLVIGLSILLLGRPDGVFVPIDQPDRWQ
                     IFIAPFASPLIWLFLAGFILAEAAARTGLDRTLAGLVFRAAGARATAVLVAAMGITFV
                     LSMFMSNTATAAMMIAVITPVLRTLPAGHAFRPALPLGIAAAANLGGMGTLVGTPPNA
                     IAAGFITRAGTELSFMDWMALGLPPALLLAAVLGAFLARRYHAAGLMLDRTALAQAQE
                     TGPRAPIWQRGVVVVVFGTTLLLWMFGPAPAAVVAFVPITVLSVTGVIGIAEIRQLRW
                     DVLILMAGGLTLGVAVTESGLAGWLVGGLPLGGLGLLGAALAFALLTAGLSNFMSNTA
                     AANILVPLGAAAAMGLGSAPGILPVIIALSASTAMCLPIATPPNAIAYSTGEINSRAF
                     LVPGLLLAGLTPLVTVPWVSWLLPRIT"
     gene            complement(3071..4666)
                     /gene="pckA"
                     /locus_tag="PLS34_07895"
     CDS             complement(3071..4666)
                     /gene="pckA"
                     /locus_tag="PLS34_07895"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRP87426.1"
                     /translation="MAARFSLQQYGIGVEEVHRNLPPAVLYEHALRWDRGSAIAASGA
                     LVAMSGVKTGRSPTDKRIIDDPNVAEDVWWGKVNMKLEQKAFEINRTQAIDYLGTRDR
                     LYVVDAYAGWDPKYTIKVRVICARPYHALFMHNMLIRPTAEQLANFGEPDYVIFNAGS
                     FPANRHVAGMTSGTSISLQFGHREMVILGTEYAGEMKKGVFTIMNYLMPARGVLSMHC
                     SATEGKDGDTSILFGLSGTGKTTLSADPKRRLIGDDEHCWSDEGIFNIEGGCYAKVID
                     LSEEAEPDIYRALRFGSVLENVVYDRDTRTVDYADTSITANTRGSYPIEYIDNVKIPC
                     MGGHPKNIIFLTCDAFGVLPPVSRLTPEQAMYHFISGYTAKVAGTEMGVTEPEATFSP
                     CFGGPFLVWHPAKYAELLAEHIRKHNSDVWLVNTGWSGGAYGTGKRMKLAYTRAIVDA
                     IHSGALAKQPTEADPIFGLAIPKACPGVPDEILVPRNTWPDKAAYDATCRKLARLFAE
                     NFQNYEDVAGRAVTDAGPRVKAA"
     gene            4873..5661
                     /locus_tag="PLS34_07900"
     CDS             4873..5661
                     /locus_tag="PLS34_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87427.1"
                     /translation="MRQPADVLDAERVAGDEPARPAGSPAARRELAWEIVSGGTGFAL
                     TIFIWFHIFDVGSLLFGPQGFDRLAEFLEGLYIAQPLVIVITLLFLAHALAAARKIPA
                     RVRERRRLNRLAKELARARRGWIGGDGLERPHEDSRLWVWQIRTGMLILVLGCFHIGM
                     MTVDTFTDFWGARHGMEAATTMERVAGGLAWFYLVLLVLLAIHASVGIYRLAVKWGVA
                     AGRSRVALKRFARIFGVAFLAAGMIVLAVMAGWLPAPFAFLLGS"
     gene            5663..>6539
                     /locus_tag="PLS34_07905"
     CDS             5663..>6539
                     /locus_tag="PLS34_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRP87428.1"
                     /translation="MKITDVLVIGAGLAGERCAIEAAAAGHSLVPPRRSHSCAAQGGM
                     QAALGNCVKAADDSPHVHFLDTVRGSDWGADQEVARMFADNAPVAVRELAHFGVPWTR
                     VRPGVNRYHIKGQQVDIEEPADKAGLIMHRDFGGTAKWRACYAAAGTGHATLYAVDSQ
                     VIRLGIDVLDRMEAIALIHDGERCHGAVVRCLRTGRLDTILARATVIASGGYGRIYGG
                     YTTNAVINEGTGASIALDTGVVPLGNMEAVQFHPTGLWPSGILITEGCRGDGGYLLNG
                     AGERFLVELEPEKQEL"
BASE COUNT         1025 a         2194 c         2316 g         1004 t
ORIGIN      
        1 gagccggcgg cgccagtcgc ggcaccggtc gcggcgcagc ccgaaacggc ggcgcccgcg
       61 gcggacaccg tggccggcgg catcccgctc gaagtcacgc tcgatcccgc gctcgcggca
      121 cgcatccccg gccccacgcc gatgttcatc atcgcccgca atccgggcgg cggcgcgccg
      181 ctggcagtga tccgcagcaa ctccaccgag ctgccgatgt cggtgacgct cagcgacgcc
      241 aacgcgatga tggcgggcgt cacgatttcc gaccaggccg aactcgagtt gatcgtgcgt
      301 gtggcgctct ccggcagtcc ggcgcagcgt cccggcgacc tgtacggtgc ggtaaactac
      361 gtccgcggca gcggtgaagc gcccacccgc atcaggatcg acagcgtcgc ggaatagcag
      421 ctgaaggagc acggcatggc ggacgatctc aaggcccgtt tcgagcaggc gtcggtggac
      481 gtgaagtcgc tgtctcagcg tcccagcgac aacgacatgc tgtcgctgta cgcgctctac
      541 aagcaggcga ccgagggcga cgtggccggc gacaagcccg gcttcttcga cttcgtcgcg
      601 cgggccaagt acgaggcctg ggaagggctg aaaggcaccg gcgccgccga ggcgatgcag
      661 aagtacatcg acaaggtgcg ctcactcggc gcctgacgcg cttgtccagc cgcctgagtt
      721 tcccggtcca gccgtcgatc gcggctgcct acctgcgcgc aatgttcgat cgccggccgc
      781 ggcgcctgcc cacggacgcc ggagtcatcc gtgtcgaggc cgaggccggc ggcgtggtgg
      841 cggacccggc gcggctcgcg gcttatcgtg atgtgtgcgg gctccccgac gacggcatgt
      901 tgccgctgac ctatccgcac atcctcgccg gcggcgtgca catggcgatg ctgctgcatc
      961 gcgacttccc ggtgcgcatg gtcgggctgg ttcacttgag caacgacatc gagctgttca
     1021 ctcccatccc cgcgggtgcg cgcctggcgc tggattgcac actggagagc ggtcgcagca
     1081 agccgcgtgg cgatgaattc cgcatggtca ccgaggcgtt gtggaacggc cagctggcct
     1141 ggcgcgaaac catgagcttc ctggccccgg cgccacgcca gcgcagcagg gcgcccggcg
     1201 aagcgccgcg gtttccgccg ctgctgggcc agtggccggt gccggccgat accgggcggc
     1261 gctacgcgcg ggtgtccggt gactggaacc cgatccacct ggcgtcgctg ctggcgcgcc
     1321 cctttggttt tcccgccgcc atcgcgcacg gcatgtggac ggtggcgcgc tgccttgcct
     1381 ggctcggcga ggggacgccc gggccgggcg cacggctgga ggtgcgcttc ctcaagccac
     1441 tgctgctgcc gggccaggtc ggcttgcacg tcgagtcacc catggagtcg ggcgatcgtg
     1501 agttctggct gttgtccacg gacggcgcca ccccccacct gaaggggctc tggaacccgg
     1561 gtacgggcta gtgagcggcc ataagtcggg cgtgcggcgg tctcaggtga tgcgcggcaa
     1621 cagccagctc acccagggca cggtgaccag cggcgtcaat cctgccaaga gcagacccgg
     1681 cacgaggaac gcgcggctgt tgatctcgcc ggtggagtag gcgatcgcgt tcggcggcgt
     1741 ggcgatcggc aggcacatgg cggtcgaggc cgacaacgcg atgatcaccg gcagaatgcc
     1801 gggcgcgctg cccagcccca tcgccgccgc cgcgccgagc ggcaccagga tattggccgc
     1861 cgccgtgttg ctcatgaagt tcgacaggcc ggccgtgagc agggcgaagg ccagcgcggc
     1921 gcccagcagc ccgagcccgc cgagcggcag ccctccgacc agccagccgg ccagccccga
     1981 ttcggtcacc gctacgccga gcgtgagccc tccggccatg aggatcagca cgtcccagcg
     2041 cagctggcgg atctcggcga tccctattac gccggtcacc gacaggacgg tgatcggcac
     2101 gaaggcgacc acggcagcgg gcgccggacc gaacatccac agcagcaggg ttgtgccgaa
     2161 caccacgacg accacgcccc gctgccagat cggtgcgcgc gggccggtct cctgggcctg
     2221 ggccagggcg gtgcgatcca gcatcagccc cgcggcgtgg tagcgacggg cgagaaaggc
     2281 gccgagcacg gcggcgagca gcagcgccgg cggcagcccg agcgccatcc agtccatgaa
     2341 cgacagctcc gtgccggcgc gcgtgatgaa cccggccgca atggcgttgg gcggcgtgcc
     2401 taccagtgtg cccatcccgc caaggttggc ggctgcggcg atgccgagcg gcagcgccgg
     2461 gcggaaggcg tggcccgccg gcagggtgcg cagcaccggc gtgatgactg cgatcatcat
     2521 cgccgcggtg gcggtgttgg acatgaacat ggacagcacg aaagtaatgc ccatggccgc
     2581 gaccagcacc gcggtggcgc gcgcgccggc ggcgcggaac accagcccgg ccagggtccg
     2641 gtccagcccc gtacgggcag ccgcttcggc caggataaag ccggccagga acagccagat
     2701 caggggactc gcaaaggggg cgatgaagat ctgccagcga tcgggctggt cgatgggcac
     2761 gaagacaccg tccggccgcc cgagcagcag gatagacagg ccgatgacca ggaacgcgac
     2821 cgcgaacgcc ggaatggcct cggtggccca caggccggcc gccagcacca ggatgaacag
     2881 gcagcgccgc ccggccgcat cgaggccgac ccagtcgggg agaaacgcca ccacggccgc
     2941 caccagcagg cagaccgata cccgccgcag cgccccgagg tcgtcgaatt cgacggccgt
     3001 gagaaagcga cgggcggcgc gccgcgtatc tctcacgact caggcctgtg ggtgcgtttc
     3061 gccagcgggc tcaggcagcc ttgacccgcg gtccggcgtc ggtgaccgcg cggccggcga
     3121 cgtcctcgta gttctggaaa ttctccgcga acagcctcgc cagcttgcga caggtcgcat
     3181 cgtaggcggc cttgtcgggc caggtgttgc gcggcacgag gatctcgtcc ggcacgcccg
     3241 gacaggcctt cggaatcgcc aggccgaaaa tgggatcggc ttccgtcggc tgcttcgcca
     3301 gtgcgccgga gtggatggcg tcgacgatgg cgcgcgtgta agccagcttc atgcgcttcc
     3361 cggtgccgta ggcgccccct gaccagcccg tgttgaccag ccacacgtcg gagttgtgct
     3421 tgcggatgtg ctccgccagc agctcggcgt acttggccgg atgccagacc aggaacggtc
     3481 cgccgaagca cggcgagaag gtcgcctcgg gctcggtcac ccccatctcg gtgccggcga
     3541 ccttggcggt gtagccgctg atgaaatggt acatcgcctg ctcgggcgtc agccggctga
     3601 ccggcggcag cacgccgaag gcgtcgcatg tcaggaagat aatgttcttc gggtgaccgc
     3661 ccatgcaggg aatcttgacg ttgtcgatgt actcgatcgg gtaggagcca cgggtgttgg
     3721 cggtgatcga ggtgtcggca tagtccacgg tccgggtatc acggtcgtac accacgtttt
     3781 cgagcaccga gccgaagcgc agcgcgcggt agatgtccgg ttcggcctcc tcggagaggt
     3841 cgatgacctt ggcgtagcag ccgccctcga tgttgaagat gccctcgtcg ctccagcaat
     3901 gctcgtcgtc gccgatcagg cggcgcttgg ggtcagcgga caaggtggtc ttgccggtgc
     3961 cggacaggcc gaacaggatc gaggtgtcgc cgtccttgcc ctcggtcgcc gagcagtgca
     4021 tggacagcac gccgcgcgcc ggcatcaggt agttcatgat ggtgaacacg cctttcttca
     4081 tctcgccggc gtactcggtg ccgagaatca ccatctcgcg atgtccgaac tgcaggctga
     4141 tgctggtgcc ggaggtcatg ccggccacgt gccggttggc cgggaagctg ccggcgttga
     4201 agatcacgta atccggctcg ccgaaattcg ccagctgctc ggccgtcggc cggatcagca
     4261 tgttgtgcat gaacagcgcg tggtaggggc gggcgcagat gacgcgcacc ttgatggtgt
     4321 acttggggtc ccagcccgca taggcgtcga ccacgtagag ccgatcacga gtgccaagat
     4381 aatcaattgc ctgcgtgcga ttgatctcga aggccttctg ctcgagcttc atgttgacct
     4441 tgccccacca gacgtcctcc gcgacgttgg ggtcgtcgat gatgcgcttg tcggtcggac
     4501 tgcggccggt cttcacaccg gacatcgcca ccagggcccc ggaggcggcg atcgcggagc
     4561 cgcgatccca gcgcagggca tgctcgtaga gcactgccgg cggcaggttg cggtgcactt
     4621 cctctacgcc gatgccgtat tgttgcaggg agaaacgcgc tgccatccga tgggcctcct
     4681 caatcgcttg ccgtgcggcg ggatccgccg gacccgccgg gaaacgccct gcgggaccgg
     4741 gggcccgggg cgtacgggtc aagcgcgtaa gtatataccg cggcctcgaa aaacccacgc
     4801 ctgcggcaat ccacagtacg cgcccggttt gggcgcggca tagactcggg ccgagagagt
     4861 acggggagcc cgatgagaca gccagcagac gtcctcgacg ccgaacgcgt cgccggcgac
     4921 gaaccagcgc gtcctgcggg aagcccggct gcccggcgcg agttggcgtg ggagatcgtc
     4981 agcggcggta ccggcttcgc gcttacgatc ttcatctggt ttcacatttt cgacgtcggc
     5041 tcactgttgt tcgggccgca gggcttcgat cggctggccg agttcctcga ggggctctac
     5101 atcgcccagc cgctcgtgat cgtcatcacg ctgctgttcc tggcccacgc gctcgccgcc
     5161 gcgcggaaga ttccggcgcg cgtgcgcgag cggcgaaggc tgaaccggct ggcgaaggag
     5221 ctggccaggg cccgtcgtgg ctggatcggc ggcgacggcc tcgagcggcc gcacgaggac
     5281 tcccggctgt gggtctggca gatccgtacg ggaatgctga tcctggtgtt gggctgcttc
     5341 cacatcggca tgatgacggt cgataccttc accgatttct ggggcgcccg gcatggcatg
     5401 gaggcggcca ccaccatgga gcgggtggcc ggcggcctgg cctggttcta cctcgtgctg
     5461 ctggtgctgc tggcgattca tgcctcggtc ggcatctacc ggctggccgt caagtggggc
     5521 gtggcggccg gccgctcccg ggtggccctg aagcgcttcg cgcggatttt cggtgtcgcg
     5581 ttcctggcgg ccgggatgat cgtgctggcg gtgatggccg gctggctgcc ggcgccattt
     5641 gccttcctgc tggggtcctg agttgaagat caccgacgta ctggtcatcg gcgccggcct
     5701 cgcgggcgag cgctgcgcca tcgaggcggc cgcggcaggg cactcgctgg tgccgccgcg
     5761 ccgcagccac agctgcgccg cgcagggcgg catgcaggcg gcactcggca actgcgtcaa
     5821 ggccgcggac gacagccccc acgtgcattt cctcgacacc gtgcgcggct cggactgggg
     5881 cgcagatcag gaagtcgcgc ggatgttcgc cgacaacgcc ccggtggcgg tgcgtgaact
     5941 ggcgcatttc ggcgtaccgt ggacgcgcgt gcggccgggc gtgaatcgct accacatcaa
     6001 gggccagcag gtggacatcg aggagccggc ggacaaggcc gggctcatca tgcaccgcga
     6061 tttcggcggc accgccaagt ggcgcgcctg ttatgcggcc gcgggcaccg gccacgccac
     6121 gctgtacgcc gtggacagcc aggtcatccg gctcggcatc gacgtgctgg atcgcatgga
     6181 agcgattgcc ctgatccacg acggcgagcg ctgccacggt gccgtggtgc gttgcctgcg
     6241 caccggccgc ctcgacacca ttctcgccag ggccactgtc atcgcctccg gcggctacgg
     6301 ccgcatctac ggcggctaca ccactaacgc ggtcatcaac gagggcaccg gcgcatcgat
     6361 cgcactcgac accggcgtgg tgccgctggg caacatggag gcggtgcagt tccacccgac
     6421 cgggctgtgg ccgtccggca tcctgatcac cgagggctgt cgcggcgacg gcggctatct
     6481 tttgaacggc gccggcgagc gcttcctggt cgagctggag cccgaaaagc aggagctgg
//
LOCUS       DAOMPG010000127         6506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5074_length_6506_cov_3.998140, whole genome shotgun sequence.
ACCESSION   DAOMPG010000127 DAOMPG010000000
VERSION     DAOMPG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5074_length_6506_cov_3.998140"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLS34_07910"
     CDS             complement(<1..661)
                     /locus_tag="PLS34_07910"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRP87429.1"
                     /translation="MSDEPSRKFSGPVVDGVTRAPSRAMLRAVGFTDADFRKPQVGIA
                     STWAEVTPCNMHIDGLAREAAAGVNQAGGKALIFNTITVSDGISMGTAGMRYSLVSRE
                     VIADSIETVVAGEGFDGFVAIGGCDKNMPGCVMAMARLDRPSVFVYGGTIRPGAQRRD
                     IVSVFEAVGGHARGVVTDAELGEVERTAIPGAGSCGGMYTANTMASAIEALGLSLPNS
                     SA"
     gene            1077..2081
                     /gene="ilvC"
                     /locus_tag="PLS34_07915"
     CDS             1077..2081
                     /gene="ilvC"
                     /locus_tag="PLS34_07915"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRP87430.1"
                     /translation="MKIYVESDVEPGNAGVKRIAVIGYGSQGRAHALNLRDSGFDVTV
                     GLRPGGASWKKAEADGIKVAPPAQAAQGADIVAVLVPDMAAPAVWRAIEGSIRPGAAA
                     LFAHGFNIHYGSIKAPDNIDVILVAPKGPGALVRRQFEEGRGVPCLMAVAQDATGRAK
                     AKALAYAHGVGGTRGGVLETTFAEETETDLFGEQAVLCGGVTELVLQGFETLVEAGYQ
                     PEVAYFECMHELKLIVDLLHEGGMAKMHQFVSDTAKYGDLSRGPRIIDEHTRQRMREV
                     LGEIRDGTFAREWQAEHEAGLPHYKAMLAADLDHPVEKVGRELRARMSWLKSDKQAAA
                     "
     gene            2078..3733
                     /gene="ilvG"
                     /locus_tag="PLS34_07920"
     CDS             2078..3733
                     /gene="ilvG"
                     /locus_tag="PLS34_07920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HRP87431.1"
                     /translation="MSMKGAELVVRALEQQGVEYIFGYPGGAIMPVYDALLNSPIKHI
                     LARHEQGAALAADAYARVTGRPAVCMATSGPGATNLVTGIANAFMDSVPLVIITGQVP
                     TVVMGTDAFQEVDVFGITMPIVKHSYIVRRAEDIPKTIAQAFQIAAEGRPGPVLVDLP
                     KDISNAVPAAAPIFEMRRNREAKADPEQIQRARRLIRASERPVLYGGGGIPMAGAVDA
                     FRAYARASGIPVVTTLKGIGAVPTRDENFLGMLGMHGNRAANQAIQESDLLICVGARF
                     DDRATGRLDGFAPQAKVIHMDADPSEIGKLRHADAGLLGDLGESLAAMPTGLAIRPWQ
                     QWCAERKAEHAWRYDAPGEYIYVPDMLRKLTDAAPDVTIACDVGQHQMWVAQHCHFDR
                     PEQHLTSAGLGTMGYGVPAGIGACLALPGKRVVTVSGDGSIMMNIQELATIRRYGLDL
                     KILLVDNSSLGMVRQWQQLFHNERYSEVDLSDNPDFVEVAKSFGIPAFRITRRDEVDG
                     AIRRLVDTEGPLLAHVCIDPKSNVWPLVPPGQSNDVMMEELSA"
     gene            3730..4005
                     /locus_tag="PLS34_07925"
     CDS             3730..4005
                     /locus_tag="PLS34_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRP87432.1"
                     /translation="MNHTLRMNVHKVEGAMIRLLGLVERRGFSVTAMNAHSDEAGQQV
                     EITMQVRSAGRSVDTLTRQIAKLYDVRSVACLTAGESQATMGESVAC"
     gene            3999..5123
                     /locus_tag="PLS34_07930"
     CDS             3999..5123
                     /locus_tag="PLS34_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRP87433.1"
                     /translation="MLSHGSLALDMDGVARVPESSLTAPPARAEARAPVVTPDDVRCA
                     MQVVGRHLAPTPLVSHALLDERAGCRMFVKLENSQGIGSFKIRGSLNLLAGMSAEDRA
                     RGLVTATRGNHGQAIAYAARMHGACCTVFVPHGNSPAKNAAIRALGAQVLEAGHDYDA
                     AMIAAEKHAARSGAVLVHQGREPAMIAGAGTVALEMIEQAQGGLDAVFVPVGAGSLAA
                     GMAVAIKAASPATRVIGVCAENAPAMHHAWHTGERHAFAATETVADGLALRIPVEETL
                     AIMQAQLDGMVLVSEREIQDAIRAYAETIHQMAEGAAAAALAAALRHREAFAGGRVGV
                     VLSGGNIDAPTLVAALAGERPQHEPRAMPYFPIGELSYGY"
     gene            5113..>6506
                     /locus_tag="PLS34_07935"
     CDS             5113..>6506
                     /locus_tag="PLS34_07935"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRP87434.1"
                     /translation="MATDIDRLVIFDTTLRDGEQAPGCSMTDREKIRVARALAELGVD
                     VIEAGFPAASDGDFESVRLIAREIQGPIICGLARCGGNDIERAWQALREARRPRLHVF
                     IATSPIHRKFKLGMSREEVVRRAVEGVRVARALCEDIEFSAEDAARTELEFLAEVVAA
                     VIDAGARTVNIPDTVGYAVPAEFAERITYLRSNVPNIDQAVLSVHCHDDLGMAVANSL
                     AAVQAGARQVECTINGIGERAGNCALEEVVMAVRTRADRLPVRTGIDTRRLAPTSRLV
                     SAVTGVSVPRNKAVVGENAFAHEAGIHQHGMLRNQETYEIMRPEDVGLGGSTLVLGKH
                     SGRHAFRSRVEALGLALDEVELDRAFIAFKTLADRKKEIFDADIEALVLRADEAGAGP
                     WRLVALGIRSGEGPRASAEVELEHADGHACREAGTGDGPVEAAFRAMERATGIEVELK
                     SFDIRNVTIGEDAQG"
BASE COUNT         1066 a         2197 c         2346 g          897 t
ORIGIN      
        1 gggccgagct gttgggcagg ctcaggccca gcgcctcgat ggccgaggcc atggtgttgg
       61 cggtgtacat gccgccgcag gagccggcgc ccgggatcgc ggtacgctcc acctcgccga
      121 gctccgcgtc cgtcacgacg ccgcgcgcat gcccgccgac cgcctcgaac accgagacga
      181 tgtcgcggcg ctgcgcgccc gggcggatcg tgccgccgta cacgaacacg ctgggtcggt
      241 cgagccgcgc catggccatc acgcagcccg gcatgttctt gtcgcagccg ccgatggcca
      301 cgaagccgtc gaagccttca ccggcgacca ctgtctctat ggaatcggcg atgacctcgc
      361 gcgagaccag cgagtagcgc attccggcgg tgcccatcga gatcccgtca gagacggtga
      421 tcgtgttgaa tatcaacgcc ttgccgcccg cctggttgac gccggccgcc gcctcgcggg
      481 ccagcccgtc gatgtgcata ttgcagggcg tgacctcggc ccaggtggag gcgatgccca
      541 cctgcggctt gcggaaatcg gcgtcggtga agcccaccgc gcggagcatg gcccggctcg
      601 gcgcgcgggt gacgccgtcc acgacgggac cggaaaactt gcgggatggc tcgtcgctca
      661 tgtcgaaatc cgccctttgc ttgctaaggt gtgatggccg ccgccggccg gccggaaacc
      721 ggacgcaatc cggactatcc gaaaaaaagt gttgacacgc cggaaacagc cgtggttaga
      781 atccgctcaa cctgattgca ccgcaacaag cgttaccgaa acaatgatcc acggcaagtc
      841 acagcgcagg gcagacacga ggcagcggcc gacggccgtc gctcgcgcgc ctgagcgctt
      901 gccgctgaac gcagtcagcg ccctccttgg cctccttatc gtgattctcg tactcgggaa
      961 gagcggggtc gtggtgtagg caggctaaga agcgaaagta ctaaaccaga gaaccccgca
     1021 acctgaaaag gctgcggggt tctcgcattc tgggctggag aaaaagggta gtgagcatga
     1081 agatttacgt cgaatcggat gtcgagccgg gcaacgccgg ggtgaagcgc atcgcggtga
     1141 tcggttatgg cagccagggg cgcgcccacg cgctgaacct gcgcgacagc ggcttcgacg
     1201 tgaccgtcgg cctgcggccg ggcggcgcga gctggaagaa ggccgaggcg gacggcatca
     1261 aggtggcgcc gccggcgcag gccgcacagg gcgccgacat cgtcgccgtg ctggtgccgg
     1321 acatggccgc gccggcggtg tggcgcgcca tcgagggcag catccgcccc ggcgccgccg
     1381 cgctgttcgc gcacggcttc aacatccatt acggctccat caaggcgccg gacaacatcg
     1441 acgtcatcct ggtggcgccg aagggccccg gcgcgctggt gcgccgccag ttcgaagagg
     1501 gccgcggcgt gccctgcctg atggcggtgg cgcaggacgc caccgggcgc gcgaaagcga
     1561 aggcgctggc ctacgcccac ggcgtcggcg gcacccgcgg cggcgtgctc gagaccacct
     1621 tcgccgagga gaccgagacc gacctgttcg gcgagcaggc ggtgctgtgc ggcggcgtca
     1681 ccgagctggt cctgcagggc ttcgagacgc tggtggaagc cggctaccag ccggaggtgg
     1741 cgtatttcga gtgcatgcac gagctgaagc tgatcgtcga cctgctgcac gagggcggca
     1801 tggcgaagat gcaccagttc gtcagcgaca cggccaagta cggcgacctg agccgcgggc
     1861 cgcgcatcat cgacgagcac acgcgccagc gcatgcgcga ggtgctgggc gagatccgcg
     1921 acggcacctt cgcgcgcgag tggcaggccg agcacgaggc cgggctgccg cactacaagg
     1981 cgatgctggc ggccgacctg gaccacccgg tcgagaaggt cgggcgcgag ctgcgtgcgc
     2041 gcatgtcgtg gctgaagtcg gacaagcagg ccgccgcatg agcatgaagg gcgcagagct
     2101 ggtggttcgc gccctcgaac aacagggcgt cgagtacatt ttcggttacc ccggcggcgc
     2161 catcatgccg gtgtacgacg ccctgctgaa ctcgcccatc aagcacatcc tggcgcgcca
     2221 cgagcagggc gccgcgctgg ccgccgacgc ctacgcgcgc gtcaccggcc ggccggcggt
     2281 ctgcatggcg acctccgggc cgggcgccac caacctggtc accggcatcg ccaacgcctt
     2341 catggactcg gtgccgctgg tcatcatcac cggccaggtg ccgaccgtgg tgatgggcac
     2401 cgatgctttc caggaggtcg acgtgttcgg catcaccatg ccgatcgtca agcacagcta
     2461 catcgtgcgg cgcgccgagg acatcccgaa gaccatcgcg caggccttcc agatcgccgc
     2521 cgaggggcgg cccggcccgg tgctggtcga cctgcccaag gacatctcca atgccgtgcc
     2581 ggccgcggcg ccgatcttcg agatgcgccg caaccgcgag gccaaggcgg acccggagca
     2641 gatccagcgc gcccgccggc tgatccgggc gtccgagcgc ccggtgctgt acggcggcgg
     2701 cggcatcccg atggctgggg cggtggacgc cttccgtgcc tacgcgcgcg ccagcggcat
     2761 cccggtggtg acgaccctga agggcatcgg cgcggtgccg acccgcgacg aaaacttcct
     2821 gggcatgctg ggcatgcacg gcaaccgggc ggccaaccag gccatccagg agtccgacct
     2881 gctgatctgc gtcggcgcgc gcttcgacga ccgcgccacc ggccggctgg acggcttcgc
     2941 gccgcaagcg aaggtcatcc acatggacgc cgacccgagc gagatcggca agctgcgcca
     3001 tgccgacgcc ggcctgctgg gtgacctggg cgagtcgctg gcggcgatgc cgaccgggct
     3061 ggcgatccgt ccctggcagc agtggtgcgc cgagcgcaag gcggagcacg catggcgcta
     3121 cgatgcgccc ggcgaataca tctacgtgcc cgacatgctg cgcaagctga ccgacgccgc
     3181 gccggacgtg accatcgcct gcgacgtcgg ccagcaccag atgtgggtgg cgcagcactg
     3241 ccatttcgac cgtcccgagc agcacctgac gagcgccggg ctgggcacca tgggctacgg
     3301 cgtgccggcg ggcatcggcg cctgcctggc gctgcccggc aagcgcgtcg tcacggtgtc
     3361 gggcgacggc tcgatcatga tgaacatcca ggagctggcc accatcaggc gctatggcct
     3421 ggacctgaag atcctgctgg tcgacaactc gtcgctgggc atggtgcgcc agtggcagca
     3481 gctgtttcac aacgagcgct acagcgaggt cgacctgtcg gacaacccgg atttcgtgga
     3541 agtcgcgaag tccttcggca tcccggcctt ccgcatcacg cgccgcgacg aggtcgacgg
     3601 tgcgatccgg cgcctggtgg acaccgaggg gccgctgctg gcgcacgtat gcatcgatcc
     3661 gaagagcaat gtctggccgc tggtgccgcc gggccagagc aatgacgtca tgatggagga
     3721 gctgagcgca tgaaccacac gctgcgaatg aatgtccaca aggtcgaagg cgcaatgatc
     3781 cggctcctgg gactcgttga acggcgcggc ttctccgtga ccgcgatgaa cgcccactcc
     3841 gacgaagccg gccagcaggt ggagatcacc atgcaggtgc gctccgccgg tcgctcggtc
     3901 gacaccctga cgcgccagat cgccaagctc tacgacgtgc gcagtgtcgc gtgtctgacg
     3961 gccggcgagt cgcaggccac catgggggag agcgtcgcat gctgagccac ggatcactgg
     4021 ccctggacat ggacggggtc gcgcgagtac ccgagagttc actgaccgcg ccgccggcgc
     4081 gggccgaggc gcgtgcgccg gtggtgacgc cggacgatgt acggtgcgcc atgcaggtgg
     4141 tcggccggca tctcgcgccc acgcccctgg tgagccacgc gctgctggac gagcgcgccg
     4201 gctgccggat gttcgtgaag ctcgagaaca gccagggcat cggctcgttc aagattcgcg
     4261 gcagcctgaa cctgctggcc gggatgagcg ccgaggatcg cgcccgcggg ctggtcacgg
     4321 cgacgcgcgg caaccacggg caggcgatcg cttatgccgc gcgcatgcac ggcgcctgct
     4381 gcacggtgtt cgtgccgcac ggcaacagcc cggccaagaa cgccgccatc agggcactcg
     4441 gcgcgcaggt gctcgaggcc ggccacgatt acgatgcggc gatgatcgcc gcggagaaac
     4501 atgccgcgcg cagcggggcc gtgctggtgc accaggggcg cgagcccgcc atgatcgccg
     4561 gcgccggcac cgtggccctg gaaatgatcg agcaggccca gggcggcctg gacgcggtgt
     4621 tcgtgccggt cggcgcgggc agcctggcgg cgggcatggc ggtggcgatc aaggccgcga
     4681 gcccggcgac gcgcgtgatt ggcgtgtgcg ctgaaaacgc tccggcgatg catcacgcct
     4741 ggcacacggg tgagcggcac gcctttgccg cgaccgaaac cgtcgctgac ggcctggcct
     4801 tgcgcatccc cgtggaggaa accctggcca tcatgcaggc acagctggac ggcatggtgc
     4861 tggtctcgga gcgcgagatc caggacgcca tccgtgcata tgccgaaacc attcaccaga
     4921 tggcggaagg tgccgcggcg gcggcgctgg ctgcggcgct gcgtcaccgc gaggcctttg
     4981 ccggcgggcg cgtcggcgtg gtgctgagcg gcggcaacat cgacgcgccg acgctggtcg
     5041 cggcgctggc gggcgaacgg ccgcagcacg agccccgcgc gatgccgtac tttccgatcg
     5101 gcgagctgtc ctatggctac tgacatcgat cggctggtca tcttcgacac cacgctgcgc
     5161 gacggcgaac aggcgccggg ctgcagcatg accgaccgcg agaagatccg cgtggcgcgt
     5221 gcgctggcgg aactgggcgt agacgtcatc gaggccggct tcccggcggc ctcggacggc
     5281 gacttcgagt cggtgcggct gatcgcccgc gagatccagg ggccgatcat ctgcggcctg
     5341 gcgcgttgcg ggggcaacga catcgaacgc gcctggcagg cgctgcgcga ggcccggcgc
     5401 ccgcgcctgc acgtgttcat cgcgaccagt cccatccatc gcaagttcaa gctcggcatg
     5461 agccgcgagg aagtcgtccg gcgcgccgtc gaaggcgtgc gggtggcgcg ggcgctgtgc
     5521 gaggacatcg agttctccgc cgaggacgcc gcgcgcaccg aactggagtt cctcgcggag
     5581 gtcgtggcgg cggtgatcga tgcaggcgcg cgcacggtga acatcccgga cacggtcggc
     5641 tacgcggtgc cggcggaatt cgccgagcgc atcacctacc tgcgcagtaa cgtgccgaac
     5701 atcgaccagg ccgtgctcag cgtgcattgt cacgacgacc tcggcatggc cgtggccaac
     5761 agcctggcgg ccgtgcaggc cggtgcgcgc caggtggagt gcaccatcaa cggcatcggc
     5821 gagcgcgccg gcaactgcgc actcgaggaa gtggtgatgg cggtgcgcac gcgcgccgac
     5881 cggctgccgg tgcgcaccgg catcgacacg cgccgcctgg cgccgaccag ccggctggtg
     5941 tcggcggtca ccggcgtctc ggtgccgcgc aacaaggccg tcgtcgggga gaatgccttc
     6001 gcccacgagg cgggcatcca ccagcacggc atgctgcgca accaggagac ctacgagatc
     6061 atgcgtcccg aggacgtcgg gctgggcggc agcacgctgg tgctgggcaa gcacagcggc
     6121 cggcatgcct tccgcagccg cgtggaggcg ctcgggctgg cgctggacga ggtcgaactg
     6181 gaccgtgctt tcatcgcctt caagacgctc gccgatcgca agaaagagat cttcgacgcg
     6241 gacatcgagg cgctggtgct gcgcgccgac gaggcgggcg ccggcccgtg gcggctggtc
     6301 gcgctgggca tccgctccgg cgagggcccg cgcgcgtccg ccgaagtcga gctggagcat
     6361 gccgacggcc atgcctgccg ggaggccggc acgggcgacg gcccggtcga ggcggcgttc
     6421 cgcgccatgg agcgcgccac cggcatcgag gtcgaactga agagcttcga catccgcaac
     6481 gtcacgatcg gcgaggacgc ccaggg
//
LOCUS       DAOMPG010000128         6497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5080_length_6497_cov_4.106799, whole genome shotgun sequence.
ACCESSION   DAOMPG010000128 DAOMPG010000000
VERSION     DAOMPG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5080_length_6497_cov_4.106799"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLS34_07940"
     CDS             complement(<1..730)
                     /locus_tag="PLS34_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HRP87435.1"
                     /translation="MSESWMIYGANGYTGELIAREAVRRGRAPVLAGRSADKVAPLAR
                     ELGLEHRCFELGSPAAVREGIAGMTLVLHCAGPFSATARPMMEACIETGAHYLDITGE
                     IAVFELAHGLDAEARRRGVLLCPGVGFDVVPTDCLAAALKAALPDATRLALGFDSRGG
                     MSPGTARTSVEGLAQGGRVRREGRLITVPLAWRTRRIDFGDGEKLAMTIPWGDVSTAW
                     HTTGIQDIEVYIPASPKLVARLKRM"
     gene            complement(727..1563)
                     /locus_tag="PLS34_07945"
     CDS             complement(727..1563)
                     /locus_tag="PLS34_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87436.1"
                     /translation="MKQQRIVITGGGSGLGRAMALDHARAGWRVALLDRDADSAATVG
                     EEIEAAGGKALALGCDVTDTESVAAAATAIRRGWHGLDVLVNNAGVAGSGTVVDTPEE
                     DWRRIMEVNLFGVVNVCRAFLAAMIRAGSGHVVNIASAAGFVSPPGVAAYNVSKAGVV
                     SLSETLRVELAPHGIGVSVVCPSFFKTNLLEDFSGSEASRQMALRLMEKSPLDADDVA
                     RIVRRGVRKKRFMIVPHAEARAILMLKRMSPDLFFTAVKRQAAGFLKAQGRPAGKRSA
                     SA"
     gene            1701..2420
                     /locus_tag="PLS34_07950"
     CDS             1701..2420
                     /locus_tag="PLS34_07950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRP87437.1"
                     /translation="MAVVYLIRHGQASFGSEDYDRLSELGRRQAGLLGAHLKRSGPHV
                     DALLSGGLRRQQDTAAEIATALGERAPEITIRNDFNEYDHIGVIRAYMPLFMTEVGGP
                     RGLEDAQVFSDHKLFEFAFRFMIKSWMDNRPHDNDALESWRDFCGRVSAGLDHVLTAH
                     GPKARVAIVTSGGTIAAALRAVLGLSNKRTLSMNWSIYNASLTQLYYGRSARHEDALL
                     LGFNNVSHLEQSGGRDLITFR"
     gene            2426..3622
                     /locus_tag="PLS34_07955"
     CDS             2426..3622
                     /locus_tag="PLS34_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP87438.1"
                     /translation="MDFQIDSELSAKLAQIRAFVIERVQPLEPLLLAGEWDALDAGIA
                     ACRAEVRARDWWAPNLPAAEGGTGQGLVTLGLVSEVLGRSPLGHFVFGCQAPDAGNAE
                     LLASHGTPAQRAQFLEPLATGEIRSCFLMTEPEFAGSNPTEMGTTAERDGDEFVINGH
                     KWFATAADGARFGICMAVTDPGADKYRRASMILVETDRPGYWLVRNIPVMGHPGRGFF
                     SHGEVRLENVRVPVENLLGEAGAGFAMAQERLGPGRIHHCMRWLGICRRALDEMIAHV
                     LKREIKPGLPLAKMGAIQDWIAESAAEVEAARALVLMTAWTIEQEGFRAARERVSMIK
                     YYTANVMQRVVDRALQAHGGLGVTDDRILAFFFREERAARIYDGPDEVHRMAVSRRLL
                     KSAAER"
     gene            3619..4701
                     /locus_tag="PLS34_07960"
     CDS             3619..4701
                     /locus_tag="PLS34_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRP87439.1"
                     /translation="MSEAGAREEARAPRAGEELDAGRVADYLGSVLPGFAGPVEIAQF
                     PGGASNLTYLVRSAAGEWVLRRPPFGTKARSAHDMGREYRILSRIHDAFPYAPQPLLF
                     CADRDVLGEEFYLMERLKGLILRRDLPAGMALSPAQADALCRNLVDVHVELHAIDPET
                     AGLGELGRPEGYVARQVAGWSERYRAARTDDVPDNEALMAWLEANRPPEAARAAIIHN
                     DYKFDNVVLGETPDGWRITGVLDWEMATLGDPLMDLGASLAYWVQADDPEPLRQIRML
                     PTHLPGMMRRAEVVEYYCARAGLAPVRFDFYYVYGLFRLAVIVQQIYFRFVLGQTKNP
                     RFAPFGQFCTVLSRSAEAVAAGKAPF"
     gene            4725..5489
                     /locus_tag="PLS34_07965"
     CDS             4725..5489
                     /locus_tag="PLS34_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008304054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87440.1"
                     /translation="MSHSLFNLDGRVALVTGASRGIGEATAHLLARHGAHVIVSSRKH
                     DSVAAVAEAIRAAGGKASALACHVGETASIDAAFALIEQEHGRLDILVNNAATNPHFG
                     HITSTPVSMLEKTVEVNVRGYFVMSQKAALLMKSHGGGSIINTSSINGVRPGPGQGIY
                     SVTKAAVISMTQAFAKECAPWKVRVNAVLPGLTDTKFAAALIENERMLNMILPLIPMG
                     RVASPEEIAPAILFFASDAASYVTGACLPVDGGFLS"
     gene            5518..5778
                     /locus_tag="PLS34_07970"
     CDS             5518..5778
                     /locus_tag="PLS34_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87441.1"
                     /translation="MKLLLALVLVWIANLPFGYWREGLVRLSPAWFVAIHAPVPLVFL
                     IREWLDLDWRLATLPLFLAAYFAGQWLGGRVRRQVARRTGGN"
     gene            5827..6090
                     /locus_tag="PLS34_07975"
     CDS             5827..6090
                     /locus_tag="PLS34_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87442.1"
                     /translation="MTEASRRRQVVEKLRRERDELKLKMHLGKAELKDEWEALERKWE
                     RLEGRMEGAAEEAREASREVGAAFGVLADELGEAYRRIRAKLG"
     gene            complement(6105..>6497)
                     /locus_tag="PLS34_07980"
     CDS             complement(6105..>6497)
                     /locus_tag="PLS34_07980"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRP87443.1"
                     /translation="MRFDLGESQVHIDYRGRQSSTHWEPGIYDLLSLRLVLSNDLARG
                     ELKHAYRIVDDRARVEEVDVQVAGRETLSTPLGDIETLRLEYRSERRDRLFRLWIAPG
                     MDGALIRLEQYEEGSLRGALEIVEYRRL"
BASE COUNT          990 a         2283 c         2232 g          992 t
ORIGIN      
        1 tcatgcgctt cagccgggcg accagcttgg gcgaggccgg gatgtagacc tcgatgtcct
       61 ggatgccggt ggtgtgccac gcggtcgaca catcgcccca tgggatggtc atggccagct
      121 tttcaccgtc gccgaaatcg atgcgccggg tgcgccaggc gagcggcacg gtgatgagcc
      181 ggccctcgcg ccgcacccgc ccgccctgtg ccagcccctc gaccgaggtg cgggcggtgc
      241 cggggctcat gccgccgcgc gaatcgaagc cgagggcgag acgcgtggcg tcgggcagcg
      301 cggccttgag cgcggccgcg aggcagtccg tgggcacgac gtcgaagcct acccccggac
      361 agagcagcac gccgcgacgc cgggcctcgg catcgaggcc gtgcgccagc tcgaacaccg
      421 cgatctcgcc ggtgatgtcg aggtaatgtg ccccggtctc gatgcaggcc tccatcatcg
      481 gccgggcggt ggcagagaac gggcccgcgc aatgcagcac cagagtcatg ccggcaatgc
      541 cctcgcggac cgcggcgggg ctgcccagct cgaagcagcg atgttccagt ccgagctcgc
      601 gcgccagcgg ggcgaccttg tccgcgctgc gtcccgccag cacgggcgcg cggccgcgcc
      661 gcacggcctc gcgcgcgatc agctcgccgg tgtagccgtt ggcgccgtag atcatccagg
      721 actcgctcat gcgctggcgc tccgcttgcc ggccggtcgg ccctgggcct tgagaaagcc
      781 cgccgcctgt cgcttgacgg cggtaaagaa caggtcgggc gacatgcgct tcagcatcag
      841 gatggcacgg gcctcggcgt gcggcacgat catgaaccgc ttcttgcgca cgccccgccg
      901 cacgatgcga gcgacgtcgt cggcatccag cggcgatttc tccatcagcc gcagggccat
      961 ctggcgcgac gcctcactgc cactgaaatc ctccagcagg ttggtcttga agaaggacgg
     1021 acacaccacg ctgacgccga tgccgtgcgg cgccagttcg acgcgcaggg tttccgacag
     1081 cgaaaccacc ccggccttcg agacgttgta ggcggcgacg cccggcggcg agacgaagcc
     1141 ggcggccgag gcgatattca ccacgtggcc ggagcccgcc cggatcatgg ccgccaggaa
     1201 ggcccggcag acgttcacga cgccgaacag gttgacctcc atgatccggc gccagtcctc
     1261 ctccggcgtg tcgaccacgg tacccgagcc ggccacgccg gcgttgttca ccagcacgtc
     1321 gaggccgtgc cagccacgcc ggatggcagt ggccgccgcg gccaccgact ccgtgtcggt
     1381 cacgtcgcag ccgagcgcca gcgccttgcc gccggccgcc tcgatctcct cgccgaccgt
     1441 cgcggcgctg tccgcgtccc ggtcgagaag ggccacgcgc cagcccgcgc gcgcatggtc
     1501 cagcgccatc gcgcggccca ggccgctgcc gccgccggta attacgatcc tctgctgttt
     1561 catcttcgca ggcccgccgt gctgtccctg aaaccgacat gatgcacgag gccgcgccca
     1621 ttgttgacac cgcgcgaagc ggggccctat ggttccggcc ctcccgacct gcttcggcta
     1681 cagcgagccc gcattttttc atggcagtcg tctacctcat ccggcacggc caggcatcct
     1741 tcggcagcga ggactacgac cggctgtccg agctcggccg ccggcaggcg ggactgctgg
     1801 gcgcgcacct caagcgcagc ggaccgcacg tcgacgccct gctcagcggc ggactgcggc
     1861 gccagcagga cacggcggcc gagatcgcca cggcgctcgg cgagcgcgcg ccggaaatca
     1921 ccatccgcaa cgatttcaac gaatacgacc acatcggcgt catccgcgcc tacatgccgc
     1981 tgttcatgac cgaggtcggc gggccgcgtg gcctcgagga cgcccaggtc ttcagtgatc
     2041 acaagctgtt cgagttcgcg tttcgcttca tgatcaagtc ctggatggac aaccggccac
     2101 acgacaacga cgcgctcgaa agctggcgcg atttctgcgg ccgggtgagc gccgggctcg
     2161 accatgtgct cactgcgcac ggccccaagg cgcgggtggc gatcgtcacc tccggcggca
     2221 ccatcgccgc cgccttgcgc gcggtcctcg gcctgtcgaa caagcggacc ctgtcgatga
     2281 actggagcat ttacaatgcg tcgctgacgc agctctatta cgggcgctcc gcgcggcacg
     2341 aggatgcgct gctgctcggt ttcaacaacg tgtcccacct ggagcagtcg ggtgggcggg
     2401 acctcatcac cttccggtag gcgccatgga ctttcagatc gattccgaac tgtcggcgaa
     2461 gcttgcgcag atccgcgcgt tcgtcatcga aagggtccag ccgctcgagc cgctgctgtt
     2521 ggcgggcgag tgggatgcgc tggatgcagg catcgccgcc tgccgcgcgg aagtcagggc
     2581 acgcgactgg tgggcgccca acctgccggc cgccgagggc ggcacggggc aggggctggt
     2641 gacgctgggc ctggtctccg aggtcctggg acgcagcccg ctcggccatt tcgtgttcgg
     2701 ctgccaggcg cccgacgccg gcaacgccga gctgctggcc tcccacggga caccggcgca
     2761 gcgcgcgcag tttctcgagc cgctggcaac cggtgagatc cgcagctgtt tcctgatgac
     2821 cgagccggag ttcgccggct ccaaccccac cgagatgggc accaccgccg aacgcgacgg
     2881 cgacgagttc gtcatcaacg gccacaagtg gttcgccacc gctgccgacg gcgcgcgctt
     2941 cggcatctgc atggccgtta ccgatccggg cgccgacaag taccgccgcg ccagcatgat
     3001 cctggtggag accgaccggc ccggctactg gctggtgcgc aacatcccgg tgatgggcca
     3061 tcccggccgc ggcttcttca gccacggcga ggtgcgcctc gagaacgtgc gcgtgccggt
     3121 ggagaacctg ctcggcgagg ccggcgccgg cttcgccatg gcgcaggagc ggctcggacc
     3181 gggccgcatc caccactgca tgcgctggct gggcatctgc cggcgggcac tggacgagat
     3241 gatcgcccac gtcctcaagc gcgagatcaa gcccggcctg ccgctggcga agatgggcgc
     3301 catccaggac tggatcgcgg agtcggccgc cgaggtcgag gcggcgcgcg cgctggtcct
     3361 gatgacggcg tggaccatcg agcaggaggg cttccgtgcc gcgcgcgagc gcgtctcgat
     3421 gatcaagtac tacaccgcca acgtcatgca acgggtggtc gaccgcgccc tgcaggccca
     3481 cggcggcctc ggcgtcaccg acgaccgtat cctggccttc ttcttccgcg aggagcgcgc
     3541 cgcgcgcatc tatgacggcc ccgacgaagt gcatcgcatg gccgtgtcgc ggcgactgct
     3601 gaagtcggcg gcggaaaggt gagcgaagcc ggcgcccggg aggaagcacg cgcaccgcgc
     3661 gccggcgagg aactcgacgc cgggcgcgtg gcggattacc tcggcagtgt gctgccgggc
     3721 ttcgccgggc cggtcgagat cgcgcagttt cccggcggcg cctccaacct gacctacctg
     3781 gtgcgcagcg ccgccggcga gtgggtgctg cgccgcccgc ccttcggcac caaggcgcgc
     3841 agcgcccacg acatgggtcg cgagtaccgt atcctgtcgc gcattcacga cgccttcccc
     3901 tacgcgccgc agccgctgct gttctgcgcc gaccgcgacg tgctgggcga ggagttctac
     3961 ctcatggagc ggctcaaggg cctgatcctg cgccgcgacc tgccggcggg gatggcgctg
     4021 tccccggccc aggccgacgc gctgtgccgc aacctggtgg acgtgcacgt ggagttgcac
     4081 gccatcgatc ccgagacggc cggcctcggc gagctcgggc ggcccgaagg ctacgtggcg
     4141 cgccaggtcg ccggctggtc ggagcgctat cgcgccgcgc gcaccgacga cgtgcccgac
     4201 aacgaggcgc tgatggcctg gctggaagcc aaccggccgc ccgaggctgc gcgcgcggcc
     4261 atcattcaca acgactacaa gttcgacaac gtggtgctgg gggagacgcc ggacggctgg
     4321 cgcatcaccg gcgtgctcga ctgggagatg gcgaccctcg gcgacccgct gatggacctc
     4381 ggcgcttccc tcgcctactg ggtccaggcc gatgatccgg agccattgcg gcagatccgc
     4441 atgctgccga cacacctgcc gggcatgatg cgccgggccg aggtcgtgga gtattactgc
     4501 gcgcgcgccg gcctcgcgcc ggtgcgcttc gatttctact atgtatacgg cctgttccgc
     4561 ctggccgtga tcgtccagca gatctacttc cgcttcgtgc tcggccagac gaagaacccg
     4621 cgcttcgcgc ccttcgggca gttctgcacc gtgttgtcgc gatcggccga ggccgtggcc
     4681 gccggcaagg caccgttctg agatccgctt ccgaggagtt cctgatgagc cattcactgt
     4741 tcaacctcga cggccgcgtc gcgctggtga ccggcgcctc gcgcggcatc ggcgaggcca
     4801 ccgcgcacct gctggcgcgc cacggcgccc acgtcatcgt cagctcgcgc aagcacgaca
     4861 gcgtcgcggc cgtcgcggag gcgatccgcg cggcgggcgg caaggcgtcc gcgctcgcct
     4921 gccacgtcgg cgagaccgcg tcgatcgacg cggccttcgc actgatcgag caagagcatg
     4981 gccggctgga catcctcgtg aacaacgccg ccaccaatcc gcacttcggc cacatcacgt
     5041 cgacgcccgt gtcgatgctg gagaagaccg tcgaggtcaa cgtgcgcggc tatttcgtga
     5101 tgtcgcagaa ggcggccctg ctgatgaagt cccacggcgg cggctccatc atcaacacct
     5161 cgtccatcaa cggcgtccgg ccgggaccgg gccagggcat ctactcggtc accaaggcag
     5221 ccgtgatttc gatgacccag gccttcgcca aggagtgcgc accctggaaa gtccgggtca
     5281 atgccgtgct gcccggcctg accgacacga agtttgccgc agcgctgatc gagaacgagc
     5341 gcatgctgaa catgatcctg ccgctgatcc cgatgggccg cgtggcgagc ccggaggaaa
     5401 tcgccccggc gatcctgttc tttgcctccg acgcagcctc gtacgtcacc ggcgcctgcc
     5461 tgccggtgga cggcggcttc ctgtcctgag gcgccggacc ggcacggcca gggaacattg
     5521 aagctgctgc tcgcgctcgt gctggtgtgg atcgccaacc tgccgttcgg ctactggcgg
     5581 gagggcctgg tccgcctgtc gccggcctgg ttcgttgcga tccacgcccc cgtgccgctg
     5641 gtgttcctga tccgcgagtg gctggatctg gactggcgcc tcgccaccct gccgctgttc
     5701 ctggcggcct attttgcagg ccagtggctg ggcgggcgcg tgcgccgcca ggtggccagg
     5761 cggactggag gaaactgagc cctggactat gctttggaca ggaatcgtgg cagcgaggtg
     5821 ggcgccatga ccgaagcatc acgccgtcgc caggtggtgg aaaagctgcg ccgggagcgg
     5881 gatgagctca agctcaagat gcacctcggc aaggccgagc tgaaggacga gtgggaggcg
     5941 ctggaacgca agtgggagcg cctcgagggg cgcatggaag gcgccgcaga agaggcccgc
     6001 gaagcctcgc gcgaggtcgg cgcggccttc ggggtgctgg ccgatgagct cggcgaggca
     6061 tatcgccgca tccgggcgaa gctcggctga gtcgaggcct ggcgctacag gcgccggtat
     6121 tccacgatct cgagagcgcc gcgcaggctg ccttcttcgt actgctcgag ccggatcagc
     6181 gcgccgtcca tgccgggggc gatccagagc ctgaacagcc ggtcgcgtcg ctcgctgcgg
     6241 tactccagcc gcagggtctc gatatcgccc agcggggtgg acagggtttc gcggccggcg
     6301 acctgtacgt cgacctcctc gacccgcgca cggtcatcga cgatccggta ggcatgcttc
     6361 agctcacctc gcgcgagatc attggacagc accagccgca ggctgagcag gtcgtagatg
     6421 cccggttccc aatgcgtgct ggactggcgg ccgcggtagt cgatgtggac ctggctttcg
     6481 cccaggtcga agcgcat
//
LOCUS       DAOMPG010000129         6495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5082_length_6495_cov_4.010404, whole genome shotgun sequence.
ACCESSION   DAOMPG010000129 DAOMPG010000000
VERSION     DAOMPG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_6495_cov_4.010404"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PLS34_07985"
     CDS             complement(<1..1396)
                     /locus_tag="PLS34_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRP87444.1"
                     /translation="MNGGARIARVLYDRGVREIFTLCGGHISPILVEAKAVGIRVTDV
                     RDEATAVFAADAHARLTGVPGVAAVTAGPGLTNTITPLKNAQLAQSPVLVLGGATATA
                     LKKRGALQDIDQMPLMTPHVKYARAVRRLRDLAPAVVDALEVSETGVPGPVFVECPVD
                     LLYDEQIIRGWYAESMPRGDKLADKAVRAWLNWHTQRLFAGGDRMPRPAAESPAVLPK
                     PDTIRDIARRVAAAQRPVMVCGSQALARAGDADLLRDAIIKLGIPVYLSGMARGLLGA
                     EHPLLFRHRRRDAIKGADLVILAGVPCDFRLDYGKQIRRSARLVSFNLSRRELRLNRR
                     PDDGLLADPGLCVRDLAEAVGDARPDRREWLAELESRDTAREADITARAAVSGEGVNP
                     LRLCREIDAVLEDDSVLVADGGDFVGTASYVVRPRGPLRWLDPGAFGTLGVGGGFAIG
                     AARTRPSAQVWILYG"
     gene            complement(1407..3116)
                     /locus_tag="PLS34_07990"
     CDS             complement(1407..3116)
                     /locus_tag="PLS34_07990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRP87445.1"
                     /translation="MRLIDRILLAARIVQWRLTWSRRNLDYRPAGELDARFMTAREAA
                     RRVPNGACCLSTGMAGNARCSTLFWAIRESFQAGGAPHDLTWIAVGGQGGRGRAPGTV
                     EELGLEGLVTRFISGHVETTRSLLALAEAGKLELHVLPQGEMCFAIESQADGIDHVLS
                     KTGVGTALDPRCGRGSAVTPGARENFIDSEGEALRFRLPRLDVAVIAAPWADADGNIY
                     FHDAATITESIEAARAAKSNGGQVLVSVAGLIPAQPARVSLPASLVDAVVVNPRQEQT
                     ILSPQRRHWRLFTVGGDGDDDAAIEKLRYINRFLKLTPVRGPVECALARLGAHVFARA
                     VEPPACVNIGVGFGEEVCRELYQSPLRPELTFTTETGVYGGLPAPGIYFGAAVNPLRM
                     ESSAWMFRHYRDHLDATVLGFLEVDARGNVNVSRRGPRITNYVGPGGLPSITAAARTC
                     IFVGTFMQGAKWRVEGARMVLEQAGTPKLIDAVSEVTFSGAEALKAGKNAWYVTTVGA
                     FRLTPGGLVLEIIMPGLDVERDIRPNCGVRFGLPPGGPVVAPASVLTGVDYALAWGVG
                     AQR"
     gene            3251..4357
                     /locus_tag="PLS34_07995"
     CDS             3251..4357
                     /locus_tag="PLS34_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRP87446.1"
                     /translation="MTNPQEALARLRAELDAIDGELVDMAARRQAIVAEIGRLKRSHR
                     QPTRDYSREREVRELARARAAHRGLDPDLGERLVGAMIEASLTSQERDRLEALGTGAG
                     RSALVIGGAGRMGRWFTEFLATQGWAVSVSDPAGPVAGVPWHEDWRAPARTAELIIVA
                     APLAATAAILAELAALRPTGLVVEIGSIKAPVAGPLAALRDAGVAVASIHPMFGPDVR
                     LLAGRHVILVDIGAPAAQEAARALFEQTSAGLAEMSLEQHDRLVAWVLGLSHATNIAF
                     FTALAGSGRSVAELAAFASTTFGAQLRVASRVAQENPELYFEIQSLNPHSIAPLQALA
                     SAAAAVEQAVAAGDGAAFGTLMRAGARYLEEPGT"
     gene            4354..>4742
                     /locus_tag="PLS34_08000"
     CDS             4354..>4742
                     /locus_tag="PLS34_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP87447.1"
                     /translation="MKRSELAGSLYDLVTGDEDARVCKDIPDAACNDQPENFFLQLGA
                     NVCTKIGDELASARLVLPWLLSAAGAPAFFIGLLVPIRESLSLLPQLFVAALIRARAV
                     RKWFWVAGSIVQGACVAAMAGAALVARL"
     assembly_gap    4743..4752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4753..5675
                     /locus_tag="PLS34_08005"
     CDS             <4753..5675
                     /locus_tag="PLS34_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP87448.1"
                     /translation="AAGWVVLGLLVLFSLARGVSSVSAKDVMGKTVSKTRRGTLTGYA
                     TAAAGLVTVAAGGWLQLRGDGSEGAAGVVGLLLLAGGLWLLGAGLYAGIREQPGATEG
                     GGNAGRDAIASLSILVSDAPFRRFVLTRALLVSTALLVPFYAALAREESDGGIGSLGG
                     LLVAAGVAATVSSGFWGRMADRSSRDVLRLAAAVAGVLSLAVAGWAVAGSPGLPGGVV
                     VVAAFFVVAIAHAGIRIGRKTYLVDMATAETRATYVAVSNTAIGVVLLAGGVFGWIAD
                     WVGTAETIGLLGLVCLAGALGASRLDEVTQ"
     gene            5703..>6495
                     /locus_tag="PLS34_08010"
     CDS             5703..>6495
                     /locus_tag="PLS34_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRP87449.1"
                     /translation="MEDNFILVGGGSAGHVQLNPRYANRHGLIAGATGTGKTVTLQVL
                     AEAFSARGVPVFLADVKGDLAGLSQAGSPHPKIDERVATIGMPPPTLAPSPVVFWDVF
                     GRGGHHLRTTVSEMGPMLLARLLELNETQEGVLHVVFRYADEEGLLLLDMPDLRALLT
                     HAADQEQEISARYGRITRASIGAIQRRLLALESQGGDSFFGEPALRLEDLMRTDLSGR
                     GIINVLDARELFHSPRVYATSLLWLLAELFEQLPEVGDPPVPRLVF"
BASE COUNT          890 a         2278 c         2324 g          993 t
ORIGIN      
        1 cgccgtagag gatccagacc tgcgccgagg gccgggtgcg cgcggcgccg atggcgaagc
       61 cgccgccgac gccgagcgtg ccgaaagcgc ccgggtcgag ccagcgcagc ggtccgcgcg
      121 gccgcacgac gtacgacgcc gtgcccacga agtcgccgcc atccgccacc agcacgctat
      181 cgtcctccag caccgcgtcg atctcgcgac acagccgcag cggattcacg ccctcaccgc
      241 tgaccgccgc gcgcgccgtg atgtccgctt cgcgagcagt gtcgcgcgac tcgagttccg
      301 ccagccactc gcgccgatcc ggccgcgcgt cgccgacggc ttccgccagg tcgcgcacgc
      361 acagccccgg gtcggccagc aggccgtcgt cgggacggcg gttcaggcgc agctcgcgtc
      421 ggctgaggtt gaaagacacc agccttgcgc tgcgccggat ctgcttgccg taatccagtc
      481 ggaagtcgca gggcacgccg gccaggatga ccaggtcggc gcccttgatg gcgtcgcgac
      541 gtcgatggcg gaacagtagc gggtgctccg cgccgagcag gccgcgcgcc atgcccgaga
      601 ggtaaaccgg gatgcccagc ttgatgatgg cgtcgcgcag caggtcggcg tcgccggccc
      661 gcgccaacgc ctggctgccg cataccatga ccggccgctg cgccgccgcc acccgcctcg
      721 cgatgtcgcg gatggtgtcg ggcttcggca ggaccgccgg cgattcggca gccggcctgg
      781 gcatgcggtc gccgcccgcg aacaggcgct gcgtgtgcca gttcagccag gcgcgcaccg
      841 ccttgtccgc cagcttgtcg ccgcgcggca tgctctccgc gtaccagccg cggatgatct
      901 gttcgtcgta gagcaggtcc accgggcact ccacgaacac cgggccgggc acgcccgtct
      961 ccgagacttc cagcgcgtcg accacggccg gcgcgagatc gcgcagccgg cgcacggcac
     1021 gcgcatattt cacgtgcggc gtcatcagcg gcatctggtc gatgtcctgc agcgcgccgc
     1081 gttttttcag cgccgtggcg gtggcgccgc cgagcaccag caccggggac tgcgccagct
     1141 gcgcgttctt gagcggcgta atggtgttgg tgagccccgg gcccgcggtg acggcggcca
     1201 cgcccggcac tcccgtgagc cgcgcatgcg catctgcggc gaacaccgcg gtggcctcgt
     1261 cgcgcacgtc cgtgacgcga atgccgacgg ctttcgcctc gaccaggatg ggggagatat
     1321 ggccgccgca cagcgtgaag atctccctca ccccacggtc gtacaggacc cgggcgatgc
     1381 gtgccccgcc attcatagat gctccttcag cgttgtgcgc cgacgcccca ggcgagggca
     1441 tagtctactc ccgtcagcac ggacgcaggc gccaccaccg gcccgccggg cggcagcccg
     1501 aagcgcacgc cgcagttcgg ccggatgtcc cgctccacgt cgaggccggg catgatgatc
     1561 tcgagcacca gcccgccggg cgtgagccgg aaagcaccga cggtggtcac ataccaggca
     1621 ttcttgcccg ccttgagcgc ctcggcgccg ctgaaggtca cctccgagac ggcgtcgatc
     1681 agcttcggcg tgccggcttg ctcgagcacc atgcgcgcgc cctcgactcg ccatttcgcg
     1741 ccctgcatga aggtgccgac gaaaatgcag gtgcgcgccg cggcggtgat gctgggcagt
     1801 ccgccgggcc cgacgtaatt ggtgatgcgc gggccgcgcc tggacacgtt cacgttgccg
     1861 cgcgcgtcca cttccaggaa gccgagcacg gtggcgtcga gatgatcgcg gtagtggcgg
     1921 aacatccagg ccgagctttc catgcgcaag gggttcacgg cagcgccgaa atagatgccc
     1981 ggtgccggca gcccgccgta cacgccggtc tcggtggtga aggtcaactc cggccgcaac
     2041 ggactctggt agagctcgcg gcacacttcc tcgccaaaac cgacaccgat gttcacgcag
     2101 gccggcggct cgacggcacg ggcgaacacg tgtgcgccaa ggcgcgccag cgcgcattcc
     2161 accgggccgc gcaccggcgt cagcttgaga aagcgattga tgtaccgcag tttctcgatg
     2221 gctgcgtcgt cgtcgccgtc gcccccgacc gtgaacaggc gccagtggcg ccgctgcggc
     2281 gagaggatgg tctgctcctg gcggggattg accaccacgg cgtcgaccag cgaggccggc
     2341 aggctgacac gcgccggctg cgccgggatc aggccggcga ccgacaccag cacctggccg
     2401 ccgttggatt tcgccgcacg tgccgcctcg atgctctcgg taattgtcgc ggcatcatgg
     2461 aaatagatgt tgccgtcggc gtccgcccag ggcgccgcaa tcaccgcgac gtccagccgc
     2521 ggcagcctga agcgcagcgc ctcgccctcg ctgtcgatga agttttcccg tgcacccggc
     2581 gtcacggccg agccgcgccc gcagcgcgga tcgagtgccg tgcccacgcc ggtcttgctc
     2641 agcacgtggt cgatgccgtc ggcctgtgac tcgatcgcaa agcacatctc gccctgcggc
     2701 aggacgtgga gctccagctt gccggcctcc gcgagggcca gcagggagcg ggtggtctcg
     2761 acgtgccccg aaatgaagcg cgtgaccagg ccttcgaggc cgagctcctc gaccgtgccc
     2821 ggagcgcggc cgcgcccacc ctggccgccg acggcgatcc aggtcaggtc gtgcggcgcg
     2881 ccgccggcct ggaagctttc gcggatcgcc cagaacagcg tcgagcagcg cgcattgccc
     2941 gccattccgg tggacaggca gcaggcgccg ttcggcaccc gccgcgccgc ctcacgcgcc
     3001 gtcatgaaac gcgcatcgag ttcgccggcc ggccggtagt ccaggttgcg gcgggaccag
     3061 gtgaggcgcc attgcacgat gcgcgccgcc agcagtattc ggtcgatcaa tcgcatgaga
     3121 ccccgaataa tgccgcatcc gcgcgcccgc caatagagga attcccgcag gatccgggcg
     3181 tcggcggacc tgaccggccg gcggcgctgg gatatgctgt cgagcagccg tttgcaggag
     3241 gatcccggca ttgaccaatc cgcaggaagc gctggcccgg ctgcgggctg agctcgacgc
     3301 catcgacggc gaactggtgg acatggcggc gcgccggcag gcgattgtcg ccgagatcgg
     3361 ccggctcaag cgcagtcacc gccagccgac ccgtgattac tcgcgcgagc gcgaagtgcg
     3421 cgaactggcg cgtgcgcgcg ccgcgcatcg cggcctcgat cccgacctcg gcgagcgcct
     3481 ggtcggcgcc atgatcgagg cgtcgctcac cagccaggaa cgggaccgcc tcgaagcgct
     3541 gggcacgggc gcgggccgca gcgcgctggt gatcggcggc gccgggcgca tgggtcgctg
     3601 gttcacggaa ttcctggcga cgcagggctg ggcggtgagt gtgtcggatc cggccggccc
     3661 ggtggccggc gtcccgtggc acgaggactg gcgcgcgccg gcgcgcactg cggaactgat
     3721 catcgtcgcc gcgccgctcg cggcgaccgc ggccattctc gcggagctgg ccgcgctccg
     3781 tcccaccggt ctggtggtcg agatcggctc catcaaggcg ccggtggcgg gtccgctggc
     3841 tgcattgcgc gacgccgggg tggcggtcgc atccattcat cccatgttcg gcccggacgt
     3901 gcggctgctg gccggccgtc acgtcatcct ggtggacatc ggtgcgccgg cggcccagga
     3961 ggcggcccgt gcgctgttcg agcagacttc cgccggactg gccgagatgt ccctcgagca
     4021 gcacgaccgg ctggtcgcct gggtgctggg cctgtcccat gcgaccaaca tcgcgttctt
     4081 cacggcgctg gccggcagcg gccgcagtgt cgccgagctg gcggccttcg cgagcacgac
     4141 cttcggcgcg cagttgcgcg tcgcctcgcg ggtggcgcag gaaaaccctg agctgtactt
     4201 cgaaatccag tccctcaacc cgcacagtat cgcgccgctg caggcgctcg ccagcgcggc
     4261 cgctgccgtg gagcaagcgg ttgcggccgg cgacggcgcc gcattcggca ccctgatgcg
     4321 ggcgggcgct cgttacctcg aggaacccgg gacttgaagc gcagcgagct ggccggctcg
     4381 ctctacgacc tcgtcaccgg tgacgaggat gcgcgcgtct gcaaggacat tccggatgcg
     4441 gcgtgcaacg accagccgga gaacttcttc ctgcagctcg gcgccaacgt ctgcaccaag
     4501 atcggggacg agctggccag cgccaggctg gtactgccct ggctgctgtc cgccgccggc
     4561 gcgccggcgt ttttcatcgg gctgctggtg ccgattcgcg agtccctgtc gctgctgccg
     4621 cagctctttg tcgcggcgct gatccgggcg cgcgccgtcc gcaagtggtt ctgggtcgcg
     4681 ggcagcatcg tgcagggcgc ctgcgtcgcg gccatggccg gggcggcgct ggtcgcccgg
     4741 ctnnnnnnnn nncagcggcc ggctgggtcg tgctcggcct gctggtgctg ttcagcctgg
     4801 cacgcggcgt gtcgtcggtg tccgccaagg atgtcatggg caagaccgtc tcgaagaccc
     4861 ggcgcggcac gctgaccggc tatgccacgg cggccgctgg cctggtgacc gtggcggccg
     4921 gcggctggct gcagttgcgc ggtgacgggt ccgagggtgc agccggggtc gtcggcctgc
     4981 tgctcctggc cggcgggctg tggttgctcg gcgcggggct gtatgccggg attcgcgagc
     5041 agccgggcgc caccgaaggt ggcggcaatg ccggtcgtga cgccatcgcc agcctgtcga
     5101 tcctggtgtc ggacgccccg tttcgccgct tcgtcctcac gcgcgcgctg ctggtctcta
     5161 cggcgctgct ggtgccgttt tatgccgccc tggcgcggga ggagagcgac ggcggcatcg
     5221 gcagcctggg cggcctgctg gtcgccgccg gggtggccgc gaccgtgagt tccggcttct
     5281 ggggccgcat ggcggatcga tccagccgcg acgtgctgcg ccttgcggcg gccgtggcgg
     5341 gtgtgctgag cctggccgtc gcgggctggg cggtggccgg ctcgccgggc ctgccgggcg
     5401 gcgtggtggt ggtggccgcc ttcttcgtgg tcgccatcgc gcacgcgggc attcgcatcg
     5461 gtcgcaagac ctacctcgtg gacatggcga cggcggaaac ccgcgcaacc tacgtggcgg
     5521 tgagcaacac cgcgattggc gtggtgctgc tggccggcgg cgtgttcggc tggatcgccg
     5581 actgggtcgg caccgccgag accatcgggc tgctcggcct cgtctgcctc gccggcgcgc
     5641 tcggtgcatc acggctcgac gaagtgacgc aataagctga atcatttgac ggggaacgga
     5701 gcatggaaga caacttcatc ctggtaggcg gcggcagcgc gggccatgtg cagctcaatc
     5761 cgcgctatgc gaaccggcac gggctgatcg ccggcgccac gggcaccggc aagaccgtca
     5821 ccctgcaagt gctcgcggag gccttctcgg cgcgcggcgt gccggtgttc ctggcggacg
     5881 tgaagggtga tctcgccggc ttgtcgcagg cggggtcgcc gcacccgaag atcgacgagc
     5941 gcgttgccac catcggcatg ccgccgccga ccctcgcgcc gtcaccggtg gtgttctggg
     6001 acgtgttcgg ccgcggtggg catcatctgc gcacgacggt ttcggagatg gggccgatgt
     6061 tgctggcccg cctgctggag ctgaacgaaa cgcaggaagg cgtgctgcac gtggtgttcc
     6121 gctatgccga cgaggaaggg ctgctgctgc tcgacatgcc ggatttgcgc gcgctgctga
     6181 cccatgcggc tgaccaggaa caggaaattt cagcgcgcta tggccgcatc acccgtgcct
     6241 cgatcggcgc catccagcga cgcctgctgg cgctggagtc gcagggcggc gacagcttct
     6301 tcggcgagcc cgcgctcagg ctcgaggacc tgatgcgcac ggacctgtcg ggccgcggca
     6361 tcatcaacgt gctggacgcc cgcgagctgt tccactcgcc gcgcgtctac gccaccagtc
     6421 tcctgtggct gctggcggaa ctgttcgagc agttgccgga agtcggcgac ccgcccgtgc
     6481 cgcggctggt gttct
//
LOCUS       DAOMPG010000130         6444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5139_length_6444_cov_3.541086, whole genome shotgun sequence.
ACCESSION   DAOMPG010000130 DAOMPG010000000
VERSION     DAOMPG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_6444_cov_3.541086"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..392
                     /locus_tag="PLS34_08015"
     CDS             96..392
                     /locus_tag="PLS34_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87450.1"
                     /translation="MPKTLNALAIAALLAFVPAAGADVLIIEKLEAAATEMPARGTSM
                     ERVQSRFGAPLSVSGPIGDPPITRWDYRDFVVVFEHRFVIHSVEKSAQPPRPAS"
     gene            468..1358
                     /locus_tag="PLS34_08020"
     CDS             468..1358
                     /locus_tag="PLS34_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HRP87451.1"
                     /translation="MNTSSILSTGLVQHAVGTDLAATRALTEAGVRDAAARGARLVLL
                     QELHQSSYFCKVEDPALFDLAEPIPGPGTEWLGELARELSVVIVGSLFERRAAGIYHN
                     TAVVLERDGRLAGRYRKAHIPDDPAYYEKFFFTPGDDDLRPIETSVGRLGVLVCWDQW
                     YPEAARLMALAGADLLLYPTAIGRSAEDPEAEQRRQQEAWETVQRGHAVANGLPVLAC
                     NRVGFEPDPSGATAGITFWGNSFACGPQGEMLARAGDAAETLVVDIDLARSERVRRMW
                     PFLRDRRIDAYGDLLLRYRD"
     gene            1355..4948
                     /gene="mfd"
                     /locus_tag="PLS34_08025"
     CDS             1355..4948
                     /gene="mfd"
                     /locus_tag="PLS34_08025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRP87452.1"
                     /translation="MKPSFQHLPLPETAGRPLHWGQLYGSAPALAIAAAAGRHDGPLL
                     VLAGSPRQADQLAAEIGFFAGADLEVLLLPDAETLPWDVFSPHPDITSRRLATLARLP
                     ALARGVVVAATQALLQRLPPPAWTATQGLDIAIADHLDTARLRRQLQDAGYLAVPQVA
                     EHGEFALRGSLLDLYPMGSELPYRIDLFDDVVDSIRSFDPATQRSLDRLERIRLLPGR
                     EYPFSADAIRSFRERFRERFPGDPSRVGLYRDIGEGIATGGLEYYLPLFFGALAELAD
                     YLPAGTLVVEAEPVGPILEQGWAAALERYEQLAHDIERPILEAGEILVPPEALATRLQ
                     GFPTVRISPFKQAHEGVDLGTASPPSLKLDPRAAEPAAALLEFLVGFEGRVLFTAESP
                     GRREMLRSLLAGHGIDVKSMDGWPEFLAGDAPRGLAVAPLENGLLLPETRLAVIAEAQ
                     LYGERIRQRLRRRRGARDPEAVIRELSDLRPGAPVVHVDYGVGRYLGLVTLEVQDMRS
                     EFLALEYAGGDKLYVPVQSLDLISRYTGASPESAPLHRLGGEQWHKARRRAAAKVRDV
                     AAELLDLHARRAARSGHGFTLSEQDYAVFRDAFPFDETPDQRQAIEQVITDMTTAQPM
                     DRVICGDVGFGKTEVALRAAFVAVQDGRQVAMLVPTTLLAQQHFQTFADRFADWPVRV
                     EALSRFRSPKEVERVLEGLQAGKVDIVIGTHKLLQHTVRFRNLGLVIIDEEHRFGVRD
                     KERLKSLRAEVDVLTLTATPIPRTMNMALGGLRDLSMITTPPTERLAVKTFVTEWHDG
                     LLREACLREIRRGGQVYFLHNEVQTIEQAAATLAELVPEARIRIAHGQMRERELEQVM
                     LDFYHRRFNVLVCTTIIESGIDIPTANTIIVQRADKLGLAQLHQLRGRVGRSHHRAYA
                     YLLTPPRNAMTSDAVKRLEAIESLEDLGAGFTLATHDLEIRGAGELLGEEQSGQIHEV
                     GFSMYMDMLQRAVAALRSGREPDLDRPLDFGVEVELGVAALLPEDYVPDVHLRLVLYK
                     RIAGAANPQVLEDLQVELIDRFGLLPPQAGTLFEVARVRQRAARLGVRRIEANAAGGS
                     LLFRPDTTVEPRALIELIQSDSRRYRLDGQDKLRFNLDLSDPELRFDTVHRLLDRLEP
                     AQGRARPITGNRPPCALTPLPYCDSLPCWAWSCPRAWRRRTLGSASTRWRS"
     gene            4873..5490
                     /locus_tag="PLS34_08030"
     CDS             4873..5490
                     /locus_tag="PLS34_08030"
                     /inference="COORDINATES: protein motif:HMM:NF022425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HRP87453.1"
                     /translation="MLGLVLPAGLAAQDAGQRLYQVEVVIFAQPEGTSVERGPRQSRE
                     PGEARQRAGLGAGLEDEFETGDEEAPAEPAAAGNPVAPLDRGYTAPSAPLALANVAAR
                     LNTGGYRLLWHQAWVQPASDATAADLNVLAALGRGRTSPDLGGAISLWQGRFLHLGME
                     LEWRSAAGLRAELRQRRRVRTGVDQYFDHPRIGVIAVVTELPATR"
     gene            complement(5487..>6444)
                     /locus_tag="PLS34_08035"
     CDS             complement(5487..>6444)
                     /locus_tag="PLS34_08035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP87454.1"
                     /translation="ALPQEAFLRALEAAGEGIVVVDGERPDHPVAWVNPAFERMSGFA
                     RTDLEGSNLRVLQGGDRDQPALLELRAALSAGLGCSVLLRNYRPDGAMYWNQLRIEPW
                     RDAEGRLWWLGFARDVSTQREMEIMLGRRDDDGEAVDRRLPESEAVDRVTGLQNERSF
                     ELALELTWFSCARDRRSLALFLFAPDYFDTYLETFGRVAGDSCLRMVARSVAAAFRRA
                     TDVSARLGDAEFAALGTDMEREVLEAHARRVCDRVRALAIRNPHAPLTRTLTFSAVVL
                     LVQPGRAPDWRGMLAAARTALAEAQLGGTERLVIREFETPRD"
BASE COUNT          912 a         2369 c         2210 g          953 t
ORIGIN      
        1 catggcgcgc cattaaagca tagggtgacg cgcgtccggt tttttccggc ggcgcgggtg
       61 gtatattcca cataatcatg gactggagac ccgccatgcc caagaccctc aatgccctgg
      121 cgatcgcggc cctgctggcc ttcgtcccgg ccgcgggcgc cgacgtgctc atcatcgaga
      181 agctcgaggc cgcggccacg gaaatgccgg cccgcgggac ttccatggaa cgcgtccaga
      241 gccgtttcgg cgcgccgctg agcgtctccg gcccgatcgg cgacccgccg atcacgcgct
      301 gggactaccg ggatttcgtg gtggtgttcg agcaccgctt cgtcatccac agcgtggaga
      361 agagcgccca gccgccgcgc cccgcctcct gatcgaggtc tccatcggtt aaacttgcac
      421 agggtttcgg tcgcaccgaa accctgtttg tttttccggg gaccccaatg aacacatcca
      481 gcatcctcag cacgggcctg gtccagcatg cggtcgggac cgatctcgcc gccacccgcg
      541 ccctcaccga agcgggtgtc cgcgacgccg ccgcgcgcgg ggcgcgcctc gtgctgctgc
      601 aggaactgca ccagagcagc tatttctgca aggtcgagga ccccgcgctg ttcgatctcg
      661 ccgagccgat tcccgggccg ggcacggagt ggctcggcga attggcgcgt gagctctcgg
      721 tggtgatcgt cggctcgctg ttcgagcggc gcgccgccgg catctatcac aacaccgccg
      781 tggtgctgga gcgggacggc cgcctcgccg gtcgttatcg caaggcgcac attccggatg
      841 atccggccta ctacgagaag tttttcttca ctcccggcga tgacgacctg cggcccatcg
      901 agacctccgt cgggcggctc ggcgtgctcg tctgctggga ccagtggtat cccgaggctg
      961 cgcggctgat ggcgttggcg ggcgcggatc tgctgctcta cccgaccgcg atcggacgct
     1021 cggccgagga ccccgaggcc gagcagcgcc gccagcagga ggcctgggag accgtgcagc
     1081 gcggccatgc cgtggccaac ggcctgccgg tgctggcctg caaccgggtc ggcttcgagc
     1141 cggatccttc cggcgccacg gcggggatca ccttctgggg caacagcttc gcctgcggtc
     1201 cacagggcga gatgctggcg cgcgccggcg acgccgccga aacgctggtc gtggatatcg
     1261 acctggctcg ctccgagcgc gtccgccgca tgtggccgtt cctgcgggac cggcgcatcg
     1321 acgcctacgg cgacctgctg ctgcgttacc gcgattgaaa ccatcgttcc agcacctgcc
     1381 cctgcctgag acggcgggcc gcccgctgca ctggggccag ttgtacggct cggcgccggc
     1441 gctggcgatc gcggccgccg cgggccggca cgacgggccg ctgctggtgc ttgccggttc
     1501 accgcgccag gcggaccagc tggccgccga aatcggcttt ttcgccggcg ccgatctcga
     1561 agtgctgctg ctgccggacg ccgagacgtt gccgtgggac gtcttttcgc cgcacccgga
     1621 catcacctcg cggcgcctcg cgaccctggc gcgcctgccc gcgctggcac ggggcgtggt
     1681 ggtcgccgcg acccaggcgt tgctgcagcg gctgccaccg cccgcctgga ccgccacgca
     1741 gggcctcgac atcgcgatcg ctgatcacct cgacaccgcc cgcctgcggc ggcagttgca
     1801 ggacgccggc tacctggcgg tgccccaggt ggccgagcac ggcgagttcg cgttgcgcgg
     1861 ctcgctgctc gatctctacc cgatgggcag cgagctgccc taccgcatcg acctgttcga
     1921 cgacgtggtg gactccatcc gctccttcga cccggcgacg cagcgctcac tggaccggct
     1981 ggaacgcatc cgcctgctgc ctgggcggga gtaccccttc tccgcggacg ccatccgcag
     2041 cttccgcgag cgctttcgcg agcgctttcc cggcgacccg tcgcgagtgg gactgtatcg
     2101 cgacatcggc gagggcatcg ccaccggcgg gctcgagtac tacctgccgc tgtttttcgg
     2161 ggcgcttgcc gagctcgccg attacctgcc ggccggcacg ctggtggtcg aggcggagcc
     2221 ggtcgggccg atcctggagc agggctgggc ggcggcgctc gagcgctacg agcagctggc
     2281 gcacgacatc gagcgcccga tcctcgaggc cggcgagatc ctggtaccgc cggaggcgct
     2341 ggccacgcgg ctgcagggct tcccgaccgt gcggatttct cccttcaagc aggcccatga
     2401 aggcgtggat ctcggcacgg cgagcccgcc gtcgctgaaa ctcgacccgc gcgccgccga
     2461 gccggccgcg gcgctgctcg agttcctggt cggtttcgag gggcgcgtgc tgttcaccgc
     2521 cgagtccccg ggccgccgcg aaatgctgcg ctcgctgctg gccggccacg gtatcgacgt
     2581 caagtcgatg gacggctggc ccgaattcct cgccggcgac gcaccccgcg gcctggcggt
     2641 ggcgccgctg gaaaacggtc tgctgctgcc cgagacgcgg ctggcggtga tcgccgaggc
     2701 gcagctctac ggcgagcgca tccgccagcg gctgcggcgc cggcgcgggg cgcgcgaccc
     2761 cgaggcggtg atccgcgagc tgtcggacct gcggccgggc gctcccgtgg tgcacgtcga
     2821 ctacggcgtg ggccgctatc tcgggctggt gacgctcgag gtgcaggaca tgcgcagcga
     2881 gttcctcgcc ctcgagtacg ccggcggcga caagctgtac gtgccggtcc agtcgctcga
     2941 cctgatcagc cgctacaccg gcgcctcgcc ggaatccgcg ccgctgcacc ggctcggcgg
     3001 cgaacagtgg cacaaggcgc gtcgccgcgc cgccgccaag gtccgcgacg tggccgccga
     3061 gctgctcgac ctgcacgccc gccgcgcggc gcgcagcggg cacggcttca cgctgtccga
     3121 gcaggactac gcggtgtttc gcgacgcctt tcccttcgac gagacgcccg accagcgcca
     3181 ggccatcgag caggtcatca ccgacatgac cacggcgcag ccgatggacc gcgtcatttg
     3241 cggcgacgtc ggcttcggca agaccgaagt cgcgctgcgc gccgccttcg tcgccgtgca
     3301 ggacggccgc caggtggcca tgctggtgcc gaccacgctg ctggcccagc agcacttcca
     3361 gactttcgcc gaccgcttcg ccgactggcc ggtgcgggtc gaggcgctgt cgcgcttccg
     3421 ctcgcccaag gaggtcgagc gcgtcctgga aggcctgcaa gcgggcaagg tcgacatcgt
     3481 catcggcacc cacaagctgc tgcagcacac ggtgcgtttc cgcaacctcg gcctggtgat
     3541 catcgacgag gaacaccgtt tcggcgtgcg cgacaaggaa cgcctgaagt cgctgcgtgc
     3601 cgaggtcgac gtgctgacgc tcaccgccac gcccatcccg cgcaccatga acatggcgct
     3661 cggcggcctg cgcgacctgt ccatgatcac caccccgccg accgagcgcc ttgcggtcaa
     3721 gactttcgtc accgagtggc acgacggcct gctgcgcgag gcctgcctgc gcgagatccg
     3781 gcgcggcggc caggtgtatt tcctgcacaa cgaagtgcag accatcgagc aggcggcggc
     3841 gacactggct gagctcgtgc ccgaggcgcg catccgcatc gcccacggcc agatgcgcga
     3901 gcgcgagctg gagcaagtca tgctcgactt ctaccaccgg cgcttcaacg tgctggtgtg
     3961 caccaccatc atcgagagcg gcatcgacat ccccaccgca aacaccatca tcgtgcagcg
     4021 cgccgacaag ctcggcctcg cccagctcca ccagctgcgt ggccgggtcg ggcgctcgca
     4081 tcaccgcgcc tacgcctacc tgctcacgcc gccgcgcaat gccatgacct cggatgcggt
     4141 caagcgcctc gaggcgatcg agtcgctgga ggatctcggc gcgggcttca ccctcgccac
     4201 gcacgacctc gagatccgcg gcgcgggcga gctgctcggc gaggagcaga gcggccagat
     4261 tcacgaagtc ggcttcagca tgtacatgga catgctgcag cgtgcggtgg cggcgctgcg
     4321 cagcggccgc gagcccgatc tcgaccggcc gctcgatttc ggcgtcgaag tcgaactggg
     4381 ggtggccgcg ctgttgccgg aggactacgt gcccgacgtc cacctgcggc tggtgcttta
     4441 caagcgcatc gccggcgccg ccaatccgca ggtgctggag gatctccagg tcgagctgat
     4501 cgatcgtttc gggctgctgc caccgcaggc cgggacgctg ttcgaagtcg cgcgcgtccg
     4561 gcagcgcgcg gcgcggctcg gcgttcgccg catcgaggcc aacgcggcgg gcggcagcct
     4621 gctgttccgc ccggacacga cggtcgagcc gcgcgcgctg atcgagctga tccagtccga
     4681 ctcccgcagg taccggctgg acggtcagga caaactgcgt ttcaatctcg acctgtccga
     4741 cccggagctc cgcttcgaca cggtccatcg cctgctcgac cggctcgagc cggcccaagg
     4801 gcgcgcccga cccatcaccg ggaatcgccc gccatgcgca ttgacaccct tgccgtactg
     4861 cgactcgttg ccatgctggg cctggtcctg cccgcgggcc tggcggcgca ggacgctggg
     4921 cagcgcctct accaggtgga ggtcgtgatc ttcgcccagc cggagggcac gagtgtggaa
     4981 cgcggtccgc gccagtcgag ggagccgggc gaggcccgtc aacgcgccgg actcggcgcc
     5041 ggcctcgagg acgagttcga gacgggcgac gaggaggcgc cggccgagcc cgccgcggcc
     5101 ggcaacccgg ttgcgccgct cgaccgaggc tataccgcgc cgagcgcgcc gctcgccctt
     5161 gccaacgtgg ctgcgcggct gaacacgggc ggttaccgcc tgctctggca ccaggcgtgg
     5221 gtgcagccgg catcggatgc cacggccgcg gatctcaacg tgctcgcggc gctgggccgg
     5281 gggcgcacga gccccgacct gggcggtgcg atcagcctgt ggcagggccg cttcctgcac
     5341 ctgggcatgg agctggagtg gcgctcggcg gccggactcc gggccgagct gcgccagcgg
     5401 cgccgcgtcc gcaccggcgt ggaccagtac ttcgaccatc cgcgcatcgg cgtgatcgcc
     5461 gtggtcaccg agctgcccgc gacgcgctag tcgcgcggcg tctcgaattc ccggatgacc
     5521 agccgctcgg tgcccccgag ctgggcctcg gccagcgccg tgcgcgccgc cgccagcatg
     5581 ccgcgccagt ccggggcccg gcccggctgt accagcagca ccaccgcact gaaggtcagc
     5641 gtccgcgtga gcggcgcgtg cgggttgcgg atcgcgagcg cgcgcacgcg gtcgcagacc
     5701 cggcgcgcat gcgcctcgag cacctcgcgt tccatgtcgg tgcccagcgc cgcgaactcg
     5761 gcatcgccga gccgtgcgga cacgtccgtg gcacgccgga aggcggcggc gacgctgcgc
     5821 gccaccatcc gcaggcagga gtcgcccgcg acccggccga acgtctccag gtaagtgtcg
     5881 aaatagtccg gtgcgaacag gaacagcgcc agcgagcgcc ggtcgcgcgc gcaggagaac
     5941 cacgtcaact cgagcgccag ctcgaagctg cgctcgttct gcaggccggt gacacgatcc
     6001 acggcctcgg actccggcag ccgccggtcg accgcctcgc cgtcatcgtc gcgccgtccg
     6061 agcatgatct ccatctcgcg ctgcgtcgag acgtcgcgcg cgaagcccag ccaccacagg
     6121 cgaccttccg cgtcacgcca tggctcgatc cggagctggt tccagtacat cgccccgtcg
     6181 ggccgatagt tccgcaacag cacgctgcaa ccgagtccag cggacagcgc cgcgcgcagc
     6241 tcgagcaggg ccggctggtc ccggtcgccg ccctgcagca cacgcaggtt gctgccctcg
     6301 agatccgtgc gcgcaaagcc ggacatgcgc tcgaaggccg gattcaccca cgccaccgga
     6361 tgatccgggc gctcgccgtc gaccaccacg atgccctcgc ccgcggcctc cagcgcccgc
     6421 aggaacgctt cctgcggcag ggcg
//
LOCUS       DAOMPG010000131         6417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5166_length_6417_cov_4.079535, whole genome shotgun sequence.
ACCESSION   DAOMPG010000131 DAOMPG010000000
VERSION     DAOMPG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5166_length_6417_cov_4.079535"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="mnmA"
                     /locus_tag="PLS34_08040"
     CDS             complement(<1..816)
                     /gene="mnmA"
                     /locus_tag="PLS34_08040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRP87455.1"
                     /translation="MTAERIIVGMSGGVDSSVAALRLLEAGHPVEGLFMSNWSEDEEG
                     YCQSAEDFQDARAVCELLGVPLHYADFSREYRERVFADFLDGYRAGVTPNPDVLCNRE
                     IKFGSFLRHARRLGAERIATGHYARTDPDGRLLRAADPNKDQTYFLHAVPRTALQRTL
                     FPLGGLCKPEVRAIAKAAGLPNHDRKDSTGICFIGERPFAEFLARHLDDRPGAIEDAD
                     GRVLGRHHGLSRYTLGQRQGLQVGGVAGAGDAPWYVVAKDAARNVLLVAQDHDH"
     gene            complement(820..1767)
                     /locus_tag="PLS34_08045"
     CDS             complement(820..1767)
                     /locus_tag="PLS34_08045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP87456.1"
                     /translation="MDYSDFKVRLADWERDEPALREVRTSVFVKEQAVSEREEWDGLD
                     AECTHAVAENAAQGVFGTGRLHPSGKIGRMAVLPEWRGQGVGAAILRRLLEEATNRGL
                     ESVYLHAQVQVLGFYSRFGFVAEGEEFEEANIPHRLMRLRLPRGGTGLAAAGEQTGTR
                     RVLSGKREFAAAVADVAAVAARSLAIFTPDLEHGIYDTKPFLETVKRLVLSRSHARIR
                     VLVSDPDRVQLSVNRFLHVGRRLSTFIEFRRLAESFQERGDAFLVADNAALVYRARAD
                     RWEGVADTREPRMARRYLGEFDQMWQHSETAQEIGELRI"
     gene            complement(1785..3152)
                     /gene="purB"
                     /locus_tag="PLS34_08050"
     CDS             complement(1785..3152)
                     /gene="purB"
                     /locus_tag="PLS34_08050"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRP87457.1"
                     /translation="MDTSPLTAVSPVDGRYAGRAARLRPVFSEYGLIRYRVAVEARWL
                     QALADEAGIVELDPVGGKPRELLAELAAGLSMEQAVRVKTIEAKTNHDVKAVEYFLKE
                     QLAAAEPEGRLREFIHFACTSEDINNLAYALMLRDARDGVLAPEQKSLVARLARLAEE
                     HAALAMLSRTHGQAASPTTLGKEFANFAWRLRRLEAGFARVEILGKINGAVGNYNAHL
                     AAYPDVDWPALARKFVESLGLTWNPYTTQIEPHDWIAEYCHALERFNTALLDLCRDFW
                     GYVSLGYFKQKLVAGEVGSSTMPHKVNPIDFENAEGNLGIANALLAHFAAKLPVSRWQ
                     RDLSDSTVLRNIGVALAHSLIAYASVMKGLERIEPDPERLAQDLDVNWEVLGEAIQTA
                     MRRHGLENPYEQLKALTRGRRVTPAQLHEFIAGLGLPGEVQHRLLQLTPAGYTGLAAA
                     LARDL"
     gene            3325..4713
                     /locus_tag="PLS34_08055"
     CDS             3325..4713
                     /locus_tag="PLS34_08055"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRP87458.1"
                     /translation="MAAQEYRIEKDSMGELRVPADALWGAQTQRAVENFPISGRRMPA
                     EFVRAVGLVKWAAAGANLELGQLDAEIAAAIRAAADAVADGDHYEQFPVDVFQTGSGT
                     STNMNANEVIASLASGIAGKAVHPNDHVNMGQSSNDVIPTAIHLSAALEIDGALLPAL
                     DHLAEVIEAKARACHGIVKTGRTHLMDAMPVRMSQELGGWAVQVRAGMDRIRATLPRL
                     TGLAQGGTAVGTGINADPAFGKLFAAQLAQRTGIGFHPAADYFEALSCQDSAVELSGQ
                     LKTVAVSLMKIANDLRWMNSGPLAGLGEIALPALQPGSSIMPGKVNPVIPEATAMVAA
                     QVIGNDATITVAGQSGSFQLNVMLPVIADRLLDSIGLLAAVSRALADRAIRGFTVNEA
                     NIRAALDRNPILVTALNPVIGYELGAATAKKAYAEGRPIREVALEMTELTAEELARLL
                     DAAALTEGGIRK"
     gene            4710..5330
                     /locus_tag="PLS34_08060"
     CDS             4710..5330
                     /locus_tag="PLS34_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRP87459.1"
                     /translation="MKALIARRLADTRPAPPEVYRVPGIAGDLPASLQRLRPARLVPA
                     AVLLPIVERPEGFTMLFTVRAPDLRHHAGQVSFPGGRLDPRDAGPREAALREAAEEVG
                     LPEHLVEVVGYLPNYLTITGFSVTPVVGFVQPAFKPAPDAKEVSEAFEVPLDCLLEPA
                     NMQLCHKRFLGVRLPYYEMPWGPHRIWGATAAMLVNFRDLLRGELQ"
     gene            5357..6115
                     /gene="mazG"
                     /locus_tag="PLS34_08065"
     CDS             5357..6115
                     /gene="mazG"
                     /locus_tag="PLS34_08065"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRP87460.1"
                     /translation="MRRLRDPERGCPWDVAQTFETIAPYTVEEAYEVADAIERGDLDA
                     LREELGDLLFQVVFHARMAEELGAFDFATVAGGLADKMRRRHPHVFGTAEIADAAAQT
                     TAWESHKARERRARGEGVLDGVPIALPALARAQKLGRRAARVGFDWPDARGVLAKLAE
                     EARELEDAIAARAGAPEAVEEELGDLLFTVANLARHLEVDAEGALRRASLKFEHRFRA
                     MEARLAAVGRRPEEADADELERLWNEAKESTPGR"
     gene            6105..>6417
                     /locus_tag="PLS34_08070"
     CDS             6105..>6417
                     /locus_tag="PLS34_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87461.1"
                     /translation="MGVEPGGAPAAPHFVYPPELPVSAERERIAAALRARQVVVVCGA
                     TGSGKTTQLPKICLELGRGAAGMIGHTQPRRIAARAVAARIAEELGPAGAALVGWKVR
                     FT"
BASE COUNT          988 a         2205 c         2263 g          961 t
ORIGIN      
        1 atggtcgtgg tcctgcgcca ccagcagcac gttgcgtgcg gcatccttgg cgacgacgta
       61 ccagggcgca tcgccggctc cggcgacgcc gcccacctgc agtccctgcc gctgacccag
      121 ggtgtagcga ctcaggccgt ggtgccggcc cagcacccgg ccgtcggcgt cctctatggc
      181 gccgggccgg tcatcgagat gccgggccag gaactccgcg aacggccgct cgccgatgaa
      241 gcagatgccg gtgctgtcct tgcggtcgtg attgggcagg cccgccgcct tggcgatggc
      301 acgcacctcg ggcttgcaca gcccgccgag cggaaacaag gtcctctgca aggccgtgcg
      361 cggcacggca tgcaggaaat acgtctggtc cttgttcggg tcggcggcgc gcagcaggcg
      421 accgtccggg tcggtgcgtg catagtggcc ggtggcgatg cgctccgcgc cgaggcgccg
      481 ggcatgccgc aggaagctgc cgaacttgat ttcccggttg cacagcacgt ccggattggg
      541 cgtcacgccg gcgcggtagc cgtccaggaa atccgcgaac actcgttcgc ggtactcgcg
      601 cgaaaaatcc gcgtagtgca gcgggacgcc cagcagttca cagaccgcac gtgcgtcctg
      661 gaagtcctcc gccgactggc agtagccttc ctcgtcctcg gaccagttgc tcatgaacag
      721 gccctcgacc gggtggccgg cctcgaggag gcgcagcgcc gccaccgagg aatccacgcc
      781 cccggacatg ccaacgatga tgcgctccgc cgtcaggact cagatcctca gctcgccgat
      841 ttcctgcgcg gtctcgctgt gctgccacat ctggtcgaac tcgccgaggt agcgacgcgc
      901 catgcgcggc tcgcgggtat ccgccacgcc ctcccagcga tcggcccgcg cgcggtacac
      961 cagcgcggcg ttgtcggcga ccaggaaggc gtcgccgcgt tcctggaagg attccgcgag
     1021 gcggcgaaac tcgatgaacg tcgacagccg ccggcccacg tgcagaaagc gattgacgct
     1081 gagctgcacc cggtccgggt cgctgaccag gacgcggatg cgggcatggc tgcgcgacag
     1141 cacgaggcgc ttgacggtct cgagaaacgg cttggtatcg tagattccgt gctcgagatc
     1201 aggcgtgaaa atcgccagcg agcgggccgc caccgcggcg acgtccgcca ccgccgcggc
     1261 gaactcgcgc ttgccgctca acacgcgccg tgtaccggtc tgttcaccgg cggcggcgag
     1321 gccggtgccg ccgcgcggca gccgcaggcg catgagccga tgcgggatgt tggcttcctc
     1381 gaactcctca ccctcggcca cgaagccgaa gcgggaatag aagccgagca cctggacctg
     1441 cgcatgcagg tagaccgatt cgagcccgcg attcgtggcc tcctcgagca gccggcgcag
     1501 gatggcggca ccgacgccct ggccgcgcca ctccggtagc accgccatgc ggccgatctt
     1561 gccggaggga tgcagccgcc cggtgccgaa caccccctgg gctgcgttct cggccacggc
     1621 gtgggtgcat tcggcatcca gcccgtccca ctcctcgcgt tcagagacgg cctgttcctt
     1681 gacgaacacg ctggtgcgca cctccctgag ggcgggctcg tcgcgctccc agtcggccag
     1741 gcgcaccttg aaatcgctgt agtccatggc tccgtgcgtt cggttcagag atcccgcgca
     1801 agcgccgcgg ccagcccggt atagccggcc ggcgtcaatt gcagcagccg gtgctggact
     1861 tcgccgggca ggccgaggcc cgcgatgaac tcgtgcagct gcgccggcgt gacgcgccgc
     1921 ccgcgcgtca gcgccttgag ctgctcatat ggattctcca gtccatggcg gcgcatggcc
     1981 gtctggatcg cttcgccgag cacttcccag ttgacgtcga gatcctgggc gagccgctcc
     2041 ggatccggct cgatgcgctc gaggcccttc atcaccgagg cataggcgat cagtgaatgg
     2101 gccagcgcca cgccgatgtt gcgcagcacg gtggaatcgg agaggtcgcg ctgccagcgg
     2161 gacacgggca gcttggccgc gaagtgggcg agcagggcgt tggcgatgcc gaggttgccc
     2221 tcggcattct cgaagtcgat cgggttgacc ttgtgcggca tggtggagga gcccacttcg
     2281 ccggccacca gcttctgctt gaaatagccg agcgagacgt agccccagaa gtcccggcag
     2341 agatccagca gcgcggtgtt gaaacgctcc agcgcatggc agtactcggc gatccagtcg
     2401 tgcggctcga tctgcgtggt gtagggattc caggtgagac cgagcgactc cacgaacttg
     2461 cgcgccagcg ccggccagtc gacgtcgggg taagcggcga ggtgtgcgtt gtagttgccg
     2521 accgcgccgt tgatcttgcc gaggatctcg acgcgggcga agccggcctc cagccgtcgc
     2581 agtcgccacg cgaagttggc gaactccttg cccagcgtgg tcggcgacgc agcctggccg
     2641 tgcgtacgcg acagcatggc gagcgcggca tgttcctcgg ccagccgcgc caggcgggcg
     2701 accagtgact tctgctccgg cgccagcacg ccgtcgcgcg catcgcgcag catcagcgcg
     2761 taggccagat tgttgatgtc ctcggacgtg caggcgaagt ggatgaactc gcgcagccgg
     2821 ccctcgggct ccgctgccgc cagctgttcc ttgagaaagt actccaccgc cttgacgtcg
     2881 tggttggtct tggcctcgat ggtcttgacc cgcaccgcct gctccatgga cagtccggcg
     2941 gccagttccg cgagcagttc gcgcggcttg ccgccgaccg gatcgagttc cacgatgccc
     3001 gcttcgtcgg ccagcgcctg cagccagcgg gcctcgaccg cgacgcggta gcgaatcagc
     3061 ccgtactcgc tgaaaaccgg ccgcaggcgg gcggcccgac cggcgtaacg accgtcgacc
     3121 ggggagacgg cagtcagggg ggaagtatcc atcgataagg tgccgaagat gttaggattg
     3181 tcgcatcata ccgcaatgcg tcaggtcgcc tgaatccacc ccgccgaggc gccggttgcg
     3241 ccgggattaa gaaggcaaaa aacttttcat ttcattagcc tgggagcaac gattcaagtt
     3301 gtttcccgtt acgggagaga gcgcatggcc gcgcaggaat atcgcatcga gaaagacagc
     3361 atgggcgagc ttcgcgtgcc cgccgacgcc ctgtgggggg cgcagaccca gcgggcggtg
     3421 gagaacttcc ccatcagcgg ccggcgcatg ccggccgagt tcgtccgcgc ggtgggcctg
     3481 gtcaagtggg ccgcagccgg cgcgaacctc gagctcggac agctggacgc cgagattgcg
     3541 gcggccattc gcgcggcggc cgacgcggtg gcggacggcg accactacga gcagtttccg
     3601 gtggacgttt tccagaccgg ctccggcacc agcaccaaca tgaacgccaa cgaggtcatc
     3661 gcatcgctgg cctcgggcat cgccggtaag gccgtgcatc ccaacgacca cgtcaacatg
     3721 ggccagagca gcaacgacgt catccccacg gcgatccacc tgagcgccgc gctcgagatc
     3781 gacggcgcgc tgctgccggc gctcgatcac ctggcggagg tgatcgaggc caaggcgcgg
     3841 gcgtgccacg gcatcgtcaa gacgggccgt acgcacctga tggacgccat gccggtgcgc
     3901 atgagccagg aactcggcgg ctgggccgtg caggtgcgtg ccggcatgga tcgcattcgc
     3961 gccacattgc cgcgcctgac aggcctggcg cagggcggca ccgcggtcgg caccggcatc
     4021 aacgccgacc cggccttcgg gaagcttttc gcagcgcaac tggcccagcg gaccggcatc
     4081 ggcttccatc ccgctgcgga ctacttcgag gcgctgagct gccaggactc cgccgtcgag
     4141 ctgtccggcc agctcaagac cgtggcggtg agcctgatga agatcgccaa cgacctgcgc
     4201 tggatgaaca gcggaccgct ggccgggctg ggcgagatcg cgctgcccgc gctgcagccg
     4261 ggctccagca tcatgcccgg caaggtcaac ccggtgatcc cagaggcgac cgccatggtg
     4321 gctgcgcagg tgatcggcaa cgacgccacc atcacggtcg ccggccagtc cggcagcttc
     4381 cagctcaacg tcatgttgcc ggtgatcgcg gatcggctgc tcgacagcat cggcctgctg
     4441 gcggccgtgt cgcgcgcact ggccgaccgc gcgattcgcg gcttcacggt gaacgaggcc
     4501 aacatccgcg ccgcgctcga tcgcaacccc atcctggtca cggcgctcaa tccggtgatc
     4561 ggctacgagc tcggtgcggc gaccgccaag aaggcctatg ccgagggccg gccgatccgc
     4621 gaggtggcgc tcgagatgac cgaactgacg gcggaggaac tggcgcgcct gctggacgcc
     4681 gcggcgctga cggagggcgg catccgcaag tgaaggccct gatcgcccgg cggctcgcgg
     4741 atacccggcc ggcgccgccg gaggtctacc gggtgccggg cattgccggc gacctgccgg
     4801 cgtcgctgca gcgcctgcgg ccggcccgcc tggtgcctgc ggccgtgctg ctgccgatcg
     4861 tcgagcgccc ggaagggttc accatgctgt tcaccgtgcg ggcgccggac ctgcggcacc
     4921 atgcgggcca ggtgagcttt cccggcggcc ggctcgatcc ccgggatgcc gggccgcggg
     4981 aggccgcgct gcgcgaggcc gccgaggagg tcgggctgcc cgaacacctg gtggaggtcg
     5041 tcggttacct gccgaattac ctcaccatca ccggcttctc ggtgaccccc gtagtcggct
     5101 tcgtgcagcc ggcgttcaag cccgcgccgg acgccaagga agtcagcgag gccttcgagg
     5161 tgccgctcga ttgcctgctc gagccggcca acatgcagct ctgccacaag cgcttcctgg
     5221 gcgtgcgcct gccgtattac gaaatgccct ggggtccgca tcggatctgg ggcgccaccg
     5281 ccgcgatgct ggtgaatttt cgcgacttgt tgcgcggtga gctgcagtga gtgagatcga
     5341 gctgctgctg gaggtgatgc gccggctgcg cgaccccgag cgcggctgtc cctgggacgt
     5401 ggcgcagacc tttgagacca tcgcgccgta caccgtggag gaagcctacg aggtcgccga
     5461 cgccatcgag cggggtgacc tggacgccct gcgcgaagag ctcggcgacc tgctgttcca
     5521 ggtggtgttc catgcccgca tggccgagga actcggcgcc tttgatttcg cgacggtggc
     5581 gggcggactg gccgacaaga tgcgccggcg ccacccgcac gtgttcggca cggccgaaat
     5641 cgccgacgcc gccgcgcaga ccaccgcctg ggagtctcac aaggcgcgcg aacgacgcgc
     5701 gcgcggcgag ggcgtgcttg acggtgtccc gatcgccctg ccggccctgg cccgggcgca
     5761 gaaactcggc cggcgggccg cgcgggtcgg cttcgactgg ccggacgcgc gcggcgtgct
     5821 ggccaagctg gccgaggagg cgcgcgagct cgaggacgcc atagcggcac gcgccggcgc
     5881 gccggaggcg gtcgaggagg agctgggcga cctgctgttc accgtcgcca acctggcgcg
     5941 ccacctggag gtcgacgccg agggtgcgct gcgccgggcc agcctgaagt tcgagcatcg
     6001 cttccgggcg atggaggcgc gtctcgccgc agtcggccgt cgccccgagg aggcggacgc
     6061 cgacgagctg gagcggctct ggaacgaggc gaaggagtcg actcctgggc gttgagccgg
     6121 gcggtgcgcc ggcggcgccg catttcgtgt atccgccgga gctgccggtc tcggccgagc
     6181 gcgagcgcat tgccgccgcg ctccgcgcgc gccaggtcgt ggtggtctgc ggcgccaccg
     6241 gctccggcaa gaccacccag ctgcccaaga tctgcctcga actcgggcgg ggtgccgccg
     6301 gcatgatcgg acacacgcag ccgcggcgca tcgccgcccg ggcggtggcg gcgcgcatcg
     6361 ccgaggaact cgggccggcc ggcgcggcgc tggtcggctg gaaggtgcgc ttcaccg
//
LOCUS       DAOMPG010000132         6397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5181_length_6397_cov_4.206086, whole genome shotgun sequence.
ACCESSION   DAOMPG010000132 DAOMPG010000000
VERSION     DAOMPG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5181_length_6397_cov_4.206086"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLS34_08075"
     CDS             complement(<1..1168)
                     /locus_tag="PLS34_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRP87462.1"
                     /translation="MGLSVGRAAREGVLWVGFALALILLWALASYDPADPGFYSTGSG
                     RGISNAAGPAGAWVASFLYVLFGHPAYLFPVLLAGAGWFALKSRAEAQPPSGRVVGLR
                     IGGFLVMLATSCGLATLHFSGAGLPETAGGIVGQLAGLGLASFLSFLGATLLLLALWL
                     AGVSLFSGVSWLAIMDRVGRVTLALVGWVQGTIESVRDRIEGRKAAQARQESVERRKQ
                     TVVRKSPPRIEPVIAPVERSERVEKERQVPLFGTPTPGEIPPLGLLDEAPPKVGGYSD
                     EALEAMSRLVELKLRDFGVEVEVVAVHPGPVVTRFELAPAPGVKVSQISNLAKDLARA
                     LSAIAVRVVEVIPGKPTVGLEIPNESRELVTLGEILTSRAYDEMKSPLALALGKD"
     assembly_gap    1334..1343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1344..2304)
                     /gene="ald"
                     /locus_tag="PLS34_08080"
     CDS             complement(<1344..2304)
                     /gene="ald"
                     /locus_tag="PLS34_08080"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRP87463.1"
                     /translation="MRIGVPREIKPLEGRVGLIPSACAELVRAGHEVYIESSAGVKSG
                     YGDDDFRAVGVEVLADAAAVYGRAEMVVKVKEPIDGDLAHLRKDHLLFCYLHLAALPE
                     LTRRLCEIGCTAVAFETVEDDRGRLPLLAPMSDIAGRLSMQVATHLLHQPNGGKGILL
                     GGVPAAKRGKVVILGGGVAGSNSAIVAAGLGAEVTVFDMDRDKLVAARAIGANVTGLY
                     PHSADVARAVAEADIVVGSVLVTGERAPHVVSEEMVRAMQPGSVIADISVDQGGCVET
                     TRPTTWTDPTYIEHGVVHFTVTNMPGAVPRTSSQALSAAIAPYA"
     gene            2417..3409
                     /gene="trxB"
                     /locus_tag="PLS34_08085"
     CDS             2417..3409
                     /gene="trxB"
                     /locus_tag="PLS34_08085"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRP87464.1"
                     /translation="MSDPKHCRVLILGSGPAGYSAAVYAARANLKPVLVTGVEQGGQL
                     MTTTDVDNWPGDDAGVQGPELMERMRRHAERFETEIVFDHIHAVDLQRRPFRLEGDSG
                     RYTCDALIIATGASARYLGLPSEERFKGRGVSACATCDGFFYRGQPVAVIGGGNTAVE
                     EALYLSNIASHVTLVHRRDALRAEKIMQQKLFAKAGPGGNVTLLWDHVVDEVLGDDSG
                     LTGVRVRSTKNRATTRDLEVTGMFVAIGHTPNTGIFAGQLDMEHGYIQVAGTGRGNAT
                     ATSVPGVFAAGDVSDPIYRQAVTSAGSGCMAALDAERWLETAGLAPPVEVARAV"
     gene            3420..4562
                     /locus_tag="PLS34_08090"
     CDS             3420..4562
                     /locus_tag="PLS34_08090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP87465.1"
                     /translation="MPVRRLSSLAEVPRAAWNALQLAGNPFLDWAYLAGLERAGAVGE
                     DAGWLPCHLVAEEAGQLRAALPLYLKSHSWGEFVFDFAWADAYHRAGLPYYPKLVAAV
                     PFTPVPGARILTAPGVDASQAAAALIGAARELAAQAGASSLHLLLPEDGQLPLLRDAG
                     LILRQDCQFHWFDEGYGDFEGFLGALTAEKRKKFRRERRRVLEQGIRFEWLAGSEIGD
                     ALFGELYRFYAASFLRRGMEPYFPPAFWASLFTDMPDSIRVVLARRGTQAVAAAIFFV
                     GGGVLYGRYWGCDEDYHSLHFETCFYQGIELALAEGCRRFEPGAQGEHKLRRGFRPVP
                     TWSGHWLANPGFARAIDAWLARERAHSNAYIEAARRHLPFRRDEPS"
     gene            4574..5302
                     /gene="aat"
                     /locus_tag="PLS34_08095"
     CDS             4574..5302
                     /gene="aat"
                     /locus_tag="PLS34_08095"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRP87466.1"
                     /translation="MRWLGPPGSPVWFPDPASALSWPNGLLAAGGDLSPERLLAAYRR
                     GVFPWYEAGQPILWWCPDPRAVILPGELHVARRLKRYLATRRFEASFDRAFGAVVAGC
                     ARRESPDVGTWITPEMALAYERLHAMGHAHSVEVWSGERLAGGLYGVAVGQVYFAESM
                     FSRVSNASKAAMIFLAQELRRRGFRLMDCQLPSRHLASMGAREISRREFLERLAGSVD
                     EPPLADDGWSRERVSIVVDAGDAS"
     gene            5396..5614
                     /gene="infA"
                     /locus_tag="PLS34_08100"
     CDS             5396..5614
                     /gene="infA"
                     /locus_tag="PLS34_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRP87467.1"
                     /translation="MAKEDAIQMEGKVIETLPNTTFRVELDNGHIVTAHISGKMRKHY
                     IRILTGDKVTVELTPYDLSKGRIVYRGR"
     gene            complement(5706..>6397)
                     /locus_tag="PLS34_08105"
     CDS             complement(5706..>6397)
                     /locus_tag="PLS34_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87468.1"
                     /translation="VSRLIGAPPGYVGFDQGGLLTEAITKTPHCVLLLDEIEKAHPEV
                     FNLLLQVMDHGTLTDNNGRKADFRNVIIVLTTNAGAQEMSRSSIGFTEQDHATDGMDA
                     IRRLFSPEFRNRLDGIIEFKALDPKTISRVVDKLVVELEAQLQDKLVTLELDDDAREW
                     IAAKGYDPKMGARPMARIIQEHIKRPLAEELLFGSLVGGGHIRVRLDAAADRLVLETE
                     PLETREPVSQD"
BASE COUNT          966 a         2279 c         2159 g          983 t
ORIGIN      
        1 tgtccttgcc gagcgccagc gccagcggcg acttcatctc gtcgtaggcc cgcgaggtga
       61 ggatctcgcc cagcgtgacc agctcgcggg actcattcgg gatctccagc ccgacggtgg
      121 gcttgccggg aatgacctcg acgacccgca cggcgatggc cgacagcgcc cgcgccaggt
      181 ccttggcgag gttgctgatc tggctgacct tgacgccggg tgccggcgcc agctcgaagc
      241 gcgtcaccac cggccccgga tgcaccgcga cgacttcgac ctcgacgccg aagtcccgca
      301 gcttgagctc caccagccgc gacatcgcct cgagcgcctc gtccgaatag ccgccgacct
      361 tgggcggcgc ctcgtcgagc aggccgagcg gcgggatttc ccccggcgtg ggcgtgccga
      421 acagcggcac ctggcgctcc ttctcgaccc gctcgctgcg ctccaccggc gcgatcaccg
      481 gctcgatgcg cggcggcgac ttgcgcacca ccgtctgttt cctgcgctcg accgactcct
      541 ggcgcgcctg ggcggccttg cggccctcga tccggtcgcg caccgactcg atcgtgccct
      601 gcacccagcc gaccagtgcc agcgtgaccc ggccgacgcg gtccatgatg gccagccagg
      661 acaccccgct gaacagcgac acgccggcca gccagagcgc cagcaacaac agcgtggcgc
      721 ccagaaagct caggaaagag gccagcccga gaccggcgag ctgcccgacg atgccgccgg
      781 cggtctccgg caggcccgcg ccggaaaaat gcagcgtggc gagaccgcag ctggtcgcga
      841 gcatcaccag gaagccgccg atcctgaggc cgaccacgcg cccggacggc ggctgcgcct
      901 cggcccgact cttcagcgca aaccagccgg cgcccgccag cagcaccgga aacaggtacg
      961 ccgggtggcc gaacagcacg tacaggaaac tggccaccca tgcgccggcc gggccggccg
     1021 cgttgctgat gccccgcccc gagcccgtgg agtaaaagcc cgggtcggcg ggatcgtagc
     1081 tggccagcgc ccagagcagg atcagcgcca gcgcgaatcc cacccacagc acgccctcgc
     1141 gggccgcccg cccgacggac agccccatga gttgcggctt gctcccattc gctttcgcca
     1201 atctattaat ccacgcgaac taaagtcgcc aagtaactgt ttttgcgatt gtacacgcta
     1261 cttgaaaagc gcggggtgga caacctcgcc ggcgtcgacg ttgatgccct tctgcagcga
     1321 gggtacgtcc cgcnnnnnnn nnntggcata gggcgcgatc gccgcggaca gcgcctgcga
     1381 cgaggtgcgc ggcaccgcgc ccggcatgtt cgtcaccgtg aagtgcacga ccccgtgctc
     1441 gatgtaggtc ggatcggtcc aggtcgtcgg gcgggtggtc tcgacgcagc cgccctggtc
     1501 gaccgagatg tcggcgatca cgctgcccgg ctgcatggcc cggaccatct cctcgctcac
     1561 cacgtgcggc gcacgttcgc cggtgaccag caccgagccc accacgatgt cggcctcggc
     1621 caccgcccgc gccacgtcgg ccgagtgcgg atacaggccg gtgacgttgg cgccgatcgc
     1681 ccgcgcggcc accagcttgt cgcgatccat gtcgaacacc gtgacttcgg cgcccaggcc
     1741 cgccgccacg atggcggagt tgctgccggc cacgccgccg ccgaggatca cgaccttgcc
     1801 gcgcttggcg gcgggcacgc cgccgagcag gatgcccttg ccgccgttgg gctggtgcag
     1861 caggtgggtg gccacctgca tggagaggcg tcccgcgatg tcgctcatcg gcgccagcag
     1921 gggcagccgg ccgcggtcgt cctccacggt ctcgaaggcc acggcggtgc agccgatttc
     1981 gcacagccgg cgggtcagct ccggcagggc cgcaagatgc aggtagcaga acagcaggtg
     2041 atccttgcgc aggtgggcca ggtcgccgtc gatcggttcc ttgaccttga ccaccatctc
     2101 cgcgcggccg tagaccgccg cagcgtccgc caggacctcg acgccgacgg cccggaaatc
     2161 gtcgtcgccg taaccgctct tcacgccggc ggacgattcg atgtacacct cgtggccggc
     2221 cctcacgagc tcggcacagg cggagggaat caggccgacc cggccctcga ggggcttgat
     2281 ctcacgcggc acaccgatac gcatagtcgc tcctcgaaag ctgcaacgcc ggccggcccg
     2341 cggcgctttg ccggaacctg ccgcttctca taacataggt gaagtgggca tgaccgatta
     2401 aaaggctgaa ttatacatga gcgacccgaa gcactgccgc gtgctgatcc tcggctccgg
     2461 ccccgccggc tacagcgcgg ccgtgtacgc cgcgcgcgcc aatctcaagc cggtgctggt
     2521 cacgggcgtc gagcaaggcg gccagctgat gaccaccacc gacgtcgaca actggccggg
     2581 cgacgacgcc ggcgtgcagg gcccggaact catggagcgc atgcgtcgcc acgcggagcg
     2641 cttcgagacc gagatcgtgt tcgatcacat ccacgccgtc gacctgcagc gccgcccgtt
     2701 ccgcctcgag ggggacagcg gccgctacac ctgcgatgcg ttgatcatcg ccaccggcgc
     2761 cagcgcccgc tatctcgggc tgccctccga ggagagattc aaggggcgcg gcgtgtcggc
     2821 ctgcgcgacc tgcgacggct tcttctaccg cggccagccc gtcgcggtga tcggcggcgg
     2881 caacaccgcc gtcgaggagg ccttgtacct gtccaacatc gcttcgcacg tgaccctggt
     2941 gcatcggcgc gacgcgctgc gggccgagaa gatcatgcag cagaagctgt tcgccaaggc
     3001 cgggccgggc ggcaacgtca cgctgctgtg ggaccacgtg gtcgacgagg tgctcggcga
     3061 cgacagcggc ctgaccggcg tgcgcgtgcg cagcacgaag aatcgtgcga ccacccgcga
     3121 cctcgaggtc accggcatgt tcgtggccat cggccacacg cccaacacgg gcattttcgc
     3181 gggccagctc gacatggagc acggctacat ccaggtggcc ggcaccgggc gcggcaacgc
     3241 cacggccacc agcgtgccgg gcgtattcgc ggccggcgac gtctccgacc ccatttaccg
     3301 gcaggccgtc acgtcggccg gctcgggctg catggccgcg ctcgatgccg agcgctggct
     3361 ggaaaccgca ggccttgcgc cgccggtgga ggtcgccagg gcggtctgag gctgccgtga
     3421 tgccggtgcg ccgactgtcg tcgctggccg aggtgccgcg cgccgcctgg aacgccctgc
     3481 agctcgccgg taatcccttc ctcgactggg cctacctggc ggggctggag cgtgccggcg
     3541 ccgtcggcga ggacgccggc tggctgccct gccacctggt agccgaggag gccgggcaac
     3601 tgcgtgccgc cctgcccctg tacctgaagt cccactcgtg gggcgagttc gtgttcgatt
     3661 tcgcctgggc cgacgcctac caccgcgccg gcctccccta ctaccccaag ctggtcgccg
     3721 ccgtaccgtt cacgccggtg cccggcgcgc ggatcctcac cgcgccgggc gtggatgcat
     3781 cgcaggcggc ggcggcactg atcggcgccg cccgcgagct ggccgcgcag gccggcgcct
     3841 cgtcgctgca cctgctgctg ccggaggacg gccagctgcc cttgctgcgt gatgccgggc
     3901 tcatcctgcg ccaggactgc cagtttcact ggttcgacga gggctatggc gatttcgagg
     3961 gattcctcgg cgcgctcacc gccgagaagc gcaagaagtt ccgccgcgaa cggcggcgcg
     4021 tgctcgaaca gggcattcgt ttcgaatggc tcgccggcag tgaaatcggc gacgcgctgt
     4081 tcggcgagct gtatcgcttt tatgccgcca gcttcctgcg gcgcggcatg gaaccctact
     4141 ttccgccggc cttctgggcg agcctgttca ccgacatgcc ggacagcatc cgcgtggtgc
     4201 tggcgcgtcg cggcacgcag gcggtcgccg cggcgatctt tttcgtcggc ggcggcgtgc
     4261 tgtacgggcg ctactggggc tgcgacgagg actatcacag cctgcacttc gagacctgct
     4321 tctaccaggg catcgagctg gcgctggcgg agggctgccg gcgcttcgag ccgggagcgc
     4381 agggcgagca caagctgcgc cgcggtttcc ggccggtgcc cacctggtcg ggccactggc
     4441 tggcgaaccc gggtttcgcg cgcgccatcg atgcgtggct ggcacgtgaa cgcgcgcaca
     4501 gcaatgccta catcgaggca gcgcgacgcc acctgccgtt ccggcgcgac gagccgtcct
     4561 gatgacgcag gggatgcgct ggctcggccc gccgggcagc ccggtgtggt ttcccgaccc
     4621 ggcctccgcg ctgagctggc cgaacggcct gctggcggcc ggcggggacc tgtcgccgga
     4681 gcgcctgctg gcggcctatc gccgcggagt gtttccctgg tacgaggccg gccagccgat
     4741 tctctggtgg tgccccgacc cgcgcgccgt gatcctgccc ggcgagttgc acgtcgcgcg
     4801 ccggttgaag cgctaccttg cgacccgtcg attcgaggca tccttcgatc gcgccttcgg
     4861 ggcggtggtc gccggctgcg cacgccggga gtcgccggac gtcggtacct ggatcacccc
     4921 ggaaatggca ctcgcctacg agcggctgca cgcgatgggc catgcgcatt ccgtggaggt
     4981 ctggtcgggc gagcgcctcg ccggcggcct ctacggcgtc gccgtgggcc aggtgtactt
     5041 cgcggaatcc atgttcagcc gggtaagcaa tgcttccaag gccgccatga tcttcctggc
     5101 gcaggagctg cgccgccgag gattccgcct gatggattgc cagctgccgt cgcgccacct
     5161 cgccagcatg ggtgcgcgtg aaatcagccg gcgggagttc ctcgagcggc tggccgggag
     5221 cgtggacgag ccacccctgg ccgacgacgg ctggtcccgc gagcgagtct caatcgtggt
     5281 cgacgccggc gacgcgtcct gacgcctgag cgcgcgccgc cgggcccgcg attgaaaagg
     5341 cgtggggctt tctgcacaat gcgcgcggtc gaagcaccgg agtatccgcc cccagatggc
     5401 gaaggaagac gcaattcaga tggaaggcaa ggtcatcgag accctgccca acaccacgtt
     5461 cagggtggaa ctggacaacg ggcacatcgt gacggcccat atctccggga agatgcgcaa
     5521 gcactacatc cggatcctca ccggcgacaa ggtcaccgtc gagctcaccc cctacgatct
     5581 ctccaagggc cgcatcgtct accgcggccg ctagaaaacc gggaaaccgg ggtcagaaac
     5641 cggggtcaga cacgggtttc gattgggaaa cccgtgtctg accccggttt ctgaccccgg
     5701 ttttctcagt cctgcgacac cggctcgcgc gtctccagcg gctccgtctc cagcaccagc
     5761 cggtccgccg cggcatccag ccgcacgcgg atgtggccgc cgccgaccag cgagccgaac
     5821 agcaactcct cggcgagcgg ccgcttgatg tgttcctgga tgatgcgggc catgggccgt
     5881 gcacccattt tcgggtcgta gcccttggcg gcgatccatt cgcgcgcatc gtcgtcgagt
     5941 tccagggtca cgagcttgtc ctgcagctgc gcctccagct cgaccaccag cttgtccacc
     6001 acccggctga tggtcttcgg gtccagcgcc ttgaactcga tgatgccgtc caggcgattg
     6061 cggaactcgg gcgagaacag ccggcggatc gcgtccatgc cgtcggtggc gtggtcctgc
     6121 tcggtgaagc cgatgctgct gcggctcatc tcctgtgcgc cggcgttggt ggtgagcacg
     6181 atgatgacgt tgcggaaatc cgccttgcga ccgttgttgt cggtcagcgt gccgtggtcc
     6241 atgacctgca gcagcaggtt gaagacctcc gggtgggctt tctcgatctc gtccagcaac
     6301 agcacgcagt gcggggtctt ggtgatggcc tcggtcagca ggccgccctg gtcgaaaccg
     6361 acgtagcccg gcggcgcgcc gatcaggcgc gacaccg
//
LOCUS       DAOMPG010000133         6393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5184_length_6393_cov_3.839539, whole genome shotgun sequence.
ACCESSION   DAOMPG010000133 DAOMPG010000000
VERSION     DAOMPG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_6393_cov_3.839539"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1058)
                     /locus_tag="PLS34_08110"
     CDS             complement(<1..>1058)
                     /locus_tag="PLS34_08110"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRP87469.1"
                     /translation="AAAAGATLVAWEPADGWGAPRLPEGCAAFPVPGLRARLGEIADR
                     FFDAPSQRLQVIGVTGTNGKTTCAHLVAGALEALGRRAGMIGTLGSGPVAQLAPSGLT
                     TPDVIEVHRVLADLAGQGVRAVAMEVSSHALDQGRVDAVRYAAAGFTNLSRDHLDYHP
                     SLEAYGESKARLFLAHAPGVSVINTGDAFGRGLLARLPAGRRRIAVGRRAADLPDAGE
                     RVLLASVAAMEDGLALALDGDFGRATIRSRLVGDFNADNLALAFGLLAGIGVPAPDAA
                     AALGRVAAPAGRMEAYLDAAREVLAVVDYAHTPDALGKALAALRCHCKGRLWCVFGCG
                     GDRDRGKRPEMGRVAEQL"
     assembly_gap    1059..1098
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1272..3002)
                     /locus_tag="PLS34_08115"
     CDS             complement(1272..3002)
                     /locus_tag="PLS34_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP87470.1"
                     /translation="MKRQRDRADFQRRFRRRMLLAAAAFVGLGVMLGARAVNLQFVDQ
                     AFFARQGDARQMRVVEIAAHRGSIVDRRGEPLAVSSPVDSVWVNPRELRNAPADVPRL
                     AKAVGRDPEWLMRAVSSQPEREFLYVRRHLPPAEAQAVAQLGIPGVHLQREYRRFYPA
                     GEVVGHVLGFTNIDDVGQEGLELAYDHWLAGEHGAKRIRRDRLGNAIEDVESIREPRP
                     GRDLRLSIDLRLQYLAYRELKRAVQTHRAQSGSMVILDVRSGEVLAMVNQPSFNPNDR
                     SQLEAARYRNRATNDIFEPGSSIKPLVVAAALSSGRFNADSMVDTAPGWFTVGAKTIE
                     DKSNLGRIDLSTLLVRSSNVGASKVALALEPDQLWSELRRFGLGEVSATGFPGESAGV
                     LMEPDLWRAIGQATLAYGYGLSVTPLQLAHAYAILGAGGRAVPVSLLAVDQPPQTRQA
                     IDADAAAAVLRMMEPVATAKGTAPQAAIAGYRVAGKTGTARKFTTGGYHADRYLSVFA
                     GVAPASEPRLAAVVMLDEPSAGVFYGGEVAGPVFSRVVGDALRILGIPPDDGGDARSV
                     GTLVQAMGRP"
     gene            complement(2999..3274)
                     /gene="ftsL"
                     /locus_tag="PLS34_08120"
     CDS             complement(2999..3274)
                     /gene="ftsL"
                     /locus_tag="PLS34_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018948922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRP87471.1"
                     /translation="MSAVRMLLLVMALAAAVVGSAVAAIDARHQARQLFIELQGLNRD
                     RDELNVEWRQLQIERSTWAAHARVEQLARDKLSMSVPDPARIEVVKP"
     gene            complement(3271..4212)
                     /gene="rsmH"
                     /locus_tag="PLS34_08125"
     CDS             complement(3271..4212)
                     /gene="rsmH"
                     /locus_tag="PLS34_08125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRP87472.1"
                     /translation="MESGATAHAPVLLGEALEALAVHAAGRYLDATLGRGGHAAAILE
                     RLGEAGRLLVVDRDPEAIRAGQRRFAGDDRVSIARGNFAELGRIAGEAGFAAGFDGVL
                     LDLGVSSPQLDDPARGFSFLRDGPLDMRMDPECGMSAAEWLADVPERELAWVLFEYGE
                     ERHSRRIARALVKARDEAPITTTGRLAELIAAAVPGREPGKHPATRSFQAIRIFINDE
                     LGALDAALEQSLGLLAPGGRLCVISFHSLEDRRVKRFIRDHSQVAEPWRGLPEIPAHA
                     RPLLRPVGRAVRAGDAEIAANPRARSAVLRVAERLAA"
     gene            complement(4212..4667)
                     /gene="mraZ"
                     /locus_tag="PLS34_08130"
     CDS             complement(4212..4667)
                     /gene="mraZ"
                     /locus_tag="PLS34_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRP87473.1"
                     /translation="MFRGANRISLDAKGRLAVPTRYRARIEERCSNQLIITVDRDYCL
                     LVYPLPEWEDIERRISRLPTLNRQARRLQRLMVGHATEVELDGQGRILLPKELREFAA
                     IDRQVMFIGQGNKFEIWDEQRWNERRDQWLTEESADEGGLAAELETFSF"
     gene            complement(4894..5258)
                     /gene="rnpB"
                     /locus_tag="PLS34_08135"
     ncRNA           complement(4894..5258)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS34_08135"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5450..6304)
                     /gene="rsmI"
                     /locus_tag="PLS34_08140"
     CDS             complement(5450..6304)
                     /gene="rsmI"
                     /locus_tag="PLS34_08140"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRP87474.1"
                     /translation="MAKTPGTLWVVATPIGNLEDLAPRAQRILAAADLVLCEDTRHSG
                     RLLAAFGIAARTSSLHEHNEERAVPGLVERLAAGENMALISDAGTPLLSDPGFRLVRA
                     AAAAGITISPVPGPSAILAALSVAGLPTDRFAFEGFLPARATARRARLEQLAAESRTL
                     VLFEAGNRITDLLTDAAEILGGERAAVVARELTKLHETVYRGSLGELAERLPTEPDAA
                     RGEIVVIIAGHQPATDEEGDAALLARLLPALLEELPPSRAVKIAAKLSGVPRREAYEL
                     ALKLAPPD"
BASE COUNT          915 a         2296 c         2217 g          925 t
ORIGIN      
        1 cagctgctcg gcgacgcgac ccatctccgg ccgcttgccg cgatcgcggt cgcccccgca
       61 gccgaatacg caccacaggc ggcccttgca atggcagcgc agcgcagcga gcgccttgcc
      121 gagcgcatcc ggcgtgtgcg catagtcgac cacggccagc acttcgcgcg ctgcgtccag
      181 gtaggcctcc atgcgcccgg ccggggccgc gacccggccc agcgccgctg cggcatccgg
      241 cgccggcacg ccgatgccgg ccagcaggcc gaaggcgagc gcgagattgt cggcattgaa
      301 gtcgccgacc agccgtgagc ggatggtggc ccggccgaag tcgccgtcca gcgccagggc
      361 caggccgtct tccatggccg cgacgctcgc cagcagcacc cgctcgccgg cgtccggcag
      421 gtccgccgcg cgacggccca cggcgatgcg gcgtcgtccg gccggcaagc gcgccagcag
      481 gccgcggcca aaggcgtcgc cggtattgat gacgctgaca ccgggggcgt gggcgaggaa
      541 cagccgcgcc ttgctctcgc cgtaggcttc gaggctcggg tgatagtcga gatgatcacg
      601 gctcaggttg gtgaaccccg ccgccgcgta gcgcaccgcg tcgacgcgcc cctggtcgag
      661 tgcatgcgag gagacctcca tcgctacggc gcgcacgccc tgcccggcga ggtccgccag
      721 gacgcgatgc acttcgatca cgtcgggggt ggtgagaccg gaaggcgcga gttgcgcgac
      781 cgggccgctg ccgagcgtgc cgatcatgcc ggcgcgccgg ccgagcgcct cgagggcacc
      841 cgccaccagg tgcgcgcagg tcgtcttgcc gttcgtgccc gtgacgccga tcacctgcag
      901 gcgttgcgag ggcgcgtcga aaaagcggtc ggcgatctcg ccgaggcgcg cgcgcaggcc
      961 cggcaccggg aaggccgcac atccctccgg caggcgcggc gcgccccagc cgtccgccgg
     1021 ctcccaggcc accagggtcg cgccggccgc ggccgcggnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnngt ggcgcagccc gtgctcgcgc gtgcccggca cggcgaagaa
     1141 cagcgcgccg ggcacgatgc gccggctgtc caggctgagg tcggccaccg gccggtccgc
     1201 cagcgcggcc ggcgccaggt cgcccatcag cgcgccgagc gtgaacctgg ctggcgtggc
     1261 ggcgacgggc atcatggacg tcccatcgcc tggacgagcg tgccgaccga gcgggcgtcg
     1321 ccaccgtcat ccggcgggat gccgagaatg cgcaacgcat cgccgaccac tcgcgagaac
     1381 accgggcccg cgacttcgcc gccgtagaac acgcctgccg agggctcgtc gagcatgacc
     1441 acggcagcca gtcgcggctc cgaggcgggc gccacgccgg cgaacaccga caggtaccgg
     1501 tcggcgtgat aaccgccggt ggtgaacttg cgcgccgtgc cggtcttgcc ggcgacgcgg
     1561 tagccggcga tggcggcctg cggcgccgtg cccttggcag tggcgaccgg ctccatcatt
     1621 ctcagcacgg ccgcggcggc atccgcgtcg atcgcctgcc gcgtctgcgg cggctggtcg
     1681 acggccagca gcgataccgg caccgcgcgg ccgcccgcgc cgagaatggc gtaggcgtgc
     1741 gccagctgca gcggcgtcac cgacagcccg tagccgtacg ccagcgtggc ctggccgata
     1801 gcgcgccaga ggtcgggctc catcaacacg ccggccgact cgccggggaa gcccgtggcc
     1861 gacacctcgc cgaggccgaa gcggcgcaac tcgctccaca gctggtccgg ctccagtgcc
     1921 agcgccacct tggaggcgcc gacgttgctg ctgcggacca gcagcgtgct caggtcgatg
     1981 cgcccgaggt tgctcttgtc ctcgatggtc ttggcgccga ccgtgaacca gcccggcgcg
     2041 gtgtcgacca tcgagtcggc attgaagcgg ccgctgctga gcgccgcggc caccaccagc
     2101 ggcttgatgc tgctgccggg ctcgaagatg tcgttggtgg cgcggttgcg gtagcgcgcc
     2161 gcttcgagct ggctgcggtc gttggggttg aagctcggct ggttgaccat cgcgagcact
     2221 tcgccgctgc gcacatcgag gatcaccatc gagccggact gggcgcgatg cgtctgcacg
     2281 gcgcgcttga gctcgcggta ggcgaggtac tgcaggcgca ggtcgatgct caggcgcagg
     2341 tcgcggccgg gccgcggctc gcggatgctc tcgacgtcct cgatggcgtt gccgaggcgg
     2401 tcgcggcgga tccgcttggc gccgtgctcg ccggccagcc agtggtcgta cgccagctcg
     2461 agaccctcct ggccgacgtc gtcgatgttg gtgaagccga gcacgtgccc gacgacttcg
     2521 ccggccgggt agaagcgccg gtactcgcgc tgcaggtgca cgccggggat gccgagctgt
     2581 gcgaccgcct gcgcctcggc cggcggcagg tgccggcgga cgtacaggaa ttcgcgctcg
     2641 ggttgcgagc tgacggcgcg catcagccac tccggatcgc ggccgacggc cttggcgagg
     2701 cgcgggacgt cggcgggcgc gttgcgcagc tcgcgcggat tgacccatac gctgtccacc
     2761 gggctgctga ccgccagcgg ctcgccgcgc cggtcgacga tcgagccgcg atgcgccgcg
     2821 atttccacca cgcgcatctg ccgcgcgtcc ccctggcgcg cgaagaaggc ctggtcgacg
     2881 aactgcaggt tgaccgcgcg cgcaccgagc atcacgccga ggccgacgaa ggcggccgcc
     2941 gcgagcagca ttcggcgccg gaagcgccgc tggaaatcgg cacggtcgcg ctgccgcttc
     3001 atggcttcac gacctcgatc cgggcggggt ccggcacgct catggacagc ttgtcacgcg
     3061 ccagctgctc gacgcgggca tgggccgccc aggtgctgcg ctcgatctgc agctggcgcc
     3121 actcgacgtt caactcgtca cgatcgcggt tcagtccctg cagctcgatg aacagctggc
     3181 gcgcctggtg gcgggcgtcg atcgccgcga ccgcggagcc gaccaccgcc gcggccagcg
     3241 ccatgaccag caatagcatg cgcacggcac tcatgcggcc agccgctcgg ccacgcgcag
     3301 cacggcgctg cgcgcgcgcg ggttggcagc gatttcggcg tcgccggcgc gcacggccct
     3361 gccgaccggc cgcagcagcg ggcgcgcatg cgccggaatt tcgggcaggc cgcgccaagg
     3421 ctccgcaacc tgcgaatgat cccgaatgaa gcgcttcacg cgccggtcct cgagcgagtg
     3481 gaagctgatc acgcatagcc ggccgccagg cgccagcagc ccgagcgact gctcgagcgc
     3541 cgcgtccagc gcgcccaact cgtcgttgat gaaaatgcgg atcgcctgga agctgcgtgt
     3601 ggccggatgc ttgcctggct cgcggccggg gactgccgcc gcgatcagct ccgccaaccg
     3661 ccccgtggtg gtgatcggcg cctcgtcacg tgccttcacc agtgcgcgcg caatgcgcct
     3721 ggaatgacgc tcctcgccat actcgaacag cacccaggcg agctcgcgct cgggcacgtc
     3781 ggccagccac tccgcggcgc tcatgccgca ctcgggatcc atgcgcatgt ccagcgggcc
     3841 gtcgcgcaga aagctgaagc cgcgcgccgg atcgtcgagc tgcggggacg acacgcccag
     3901 gtcgagcagc acgccgtcga agccggcagc aaagccggcc tcaccggcga ttcgccccag
     3961 ctcggcgaaa ttccccctgg caatcgacac tctatcgtcc ccggcaaacc gccgctgtcc
     4021 cgcccggatg gcttccgggt cgcgatcaac gaccagcaat ctccccgcct caccaagccg
     4081 ctcgaggatc gccgccgcat gcccgccgcg ccccagcgtg gcatcgaggt agcgtcctgc
     4141 tgcgtgcacg gcgagggcct ccagcgcctc cccgaggagc accggcgcat gtgcagttgc
     4201 gcccgactcc actagaaact gaacgtttcg agttcagccg cgagtccgcc ctcgtcggcg
     4261 gactcctcgg tcaaccattg gtcgcggcgc tcgttccagc gctgctcgtc ccagatctcg
     4321 aacttgttgc cctggccgat gaacatcacc tgccggtcga tggcggcgaa ttcgcgcagc
     4381 tccttcggca gcaggatccg cccctgcccg tccagctcga cctcggtggc gtggccgacc
     4441 atcagtcgct gcaggcggcg cgcctggcga ttcagcgtgg gcagtcggga gatacgacgc
     4501 tcgatgtcct cccattcggg gagcgggtac accagcaggc agtagtcgcg gtcgacggtg
     4561 atgatcagct ggttgctgca gcgctcttcg atgcgcgcgc gatagcgcgt cgggaccgcg
     4621 aggcgaccct tggcatccag cgaaattcga ttggcgccgc gaaacatatt ggtggcccga
     4681 gggagcaagc tcccatttat tcccacttct ccccacttgg cgccactata tgaaccggca
     4741 tgccccccgt caaccgcctc ggggaagaaa tttcttttgg ctaccgatac ttaggcgcgt
     4801 ttcgaaaagt ggtttcgggc cccatcgtct ggcgaaaaat cagtcagtta agagcacttc
     4861 ctattaaagt gccttaggaa agcgcaaagg agaagaggga gccggcctgt aagccgggtt
     4921 ctgtcgagga cagtcattcc tctgggacgc acgtcaccgt gcgcctcaag caacctaccc
     4981 ggaagcccgc gcgggccgcg cgtcgcgggc gaacccgcat gcttccctat ttggtcttgc
     5041 tccgagtggg gtttaccgtg ccgcgaaccg ttaccggccg cgcggtgcgc tcttaccgca
     5101 ccttttcacc cttgccggcg cccggaggcg cttgggcggt atgttttctg tggcactttc
     5161 cgtcggctca cgccgcccag gcgttacctg gcaccctgcc ctgcggagcc cggactttcc
     5221 tccccggccg aggccggagc gactgcccgg ccgactcccg gctgccattg tacgcctgtc
     5281 cattcgaccg cgggggcgtg ccgccatcgt tgctgtcgta gcgggtgcgg ggtgtcatag
     5341 gccggccgag cgagtttttc cttgtccgag cgcaggccgg cctatggcgc cgcgcgcccg
     5401 cgggcaaccg gtgtcaggcc cgggcttcgc cgcggtagag ccatgcgcgt caatcgggcg
     5461 gcgcgagctt cagggcaagc tcgtaggcct cgcggcgcgg cacgccgctg agcttggccg
     5521 cgatcttcac agcgcgactc ggcggcagct cctcgaggag tgccggcagc agccgcgcca
     5581 gcagcgctgc gtcgccctcc tcgtccgtgg ccggctgatg tccggcgatg atcaccacga
     5641 tctcgccgcg cgctgcgtcg ggctcggtcg gcaggcgctc ggccagctcg cccaggctgc
     5701 cgcgatagac cgtctcgtgg agcttggtga gctcgcgcgc aacgaccgcg gcgcgctcgc
     5761 cgccgagtat ttcggccgcg tcggtcagca ggtcggtgat gcgattcccg gcctcgaaca
     5821 acaccagcgt gcgcgactcc gcggccagct gctcgaggcg cgcgcggcgc gcggttgcgc
     5881 gtgccggcag gaagccctcg aaggcgaaac gatcggtcgg caggccggcg accgacaagg
     5941 cggcgaggat ggccgagggg ccgggcaccg ggcttatggt gatccccgcg gccgcggcgg
     6001 cgcgcaccag gcgaaagccg ggatcgctga gcaggggcgt gccggcgtcc gagatgaggg
     6061 ccatgttctc gccggccgct agccgctcca ccagtcccgg cacggcgcgt tcctcgttgt
     6121 gctcgtgcag ggaggacgtg cgcgccgcga tgccgaaggc cgccagcagt cggccgctgt
     6181 ggcgcgtatc ctcgcagagc acgaggtcgg ccgcggcgag gatgcgctgc gctcgcggcg
     6241 cgaggtcctc gagattgccg atgggcgtcg cgacgaccca cagcgtacct ggtgtttttg
     6301 ccactataat cgcccttccg ctgaccacgc ccggaccgga aactccctcg catgctgcaa
     6361 cttccgcatc gcacagcgca aaccctgtcg gcc
//
LOCUS       DAOMPG010000134         6373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5202_length_6373_cov_4.304368, whole genome shotgun sequence.
ACCESSION   DAOMPG010000134 DAOMPG010000000
VERSION     DAOMPG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_6373_cov_4.304368"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLS34_08145"
     CDS             complement(<1..224)
                     /locus_tag="PLS34_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRP87475.1"
                     /translation="MADKRSKFIVIGLGRFGAALARRLAEHEHRVTGLDIDEAAVQRV
                     EGALSSALVADGADEEVLKTLDVAGAAAVF"
     gene            complement(217..1599)
                     /locus_tag="PLS34_08150"
     CDS             complement(217..1599)
                     /locus_tag="PLS34_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRP87476.1"
                     /translation="MQQGLVKFVGALAGYPARMLFAWYLGLVAVGTALLLLPVCHAVG
                     ADPLDFSDALFTATSAASVTGLMVRSMATDLSFTGQLVVLLLIQLGGLGIMSIGTLLF
                     VSLTGRQPVQYRLLTSESLGAPLGANIGRLILLVLAVSLAVEAVGAAALLLARLGDGP
                     FLDILWWAVFHAVSGFCNSGIALQDASLAPWAGDPAIILTMSALIILGGLGFPVLLDM
                     LHFRRRGGDRRGLRFHSQLVITATLVLLAGGTLAIWLAERHGALAGLPAGTALMNAFF
                     QGVTARTAGFTTLPVEHFAYPTLFLLLLLMFIGGGACSTAGGVKVSTVAVLTVEGVSL
                     LRRRWQAAAFRHGIPTRVLATAAAVVVVYALVLLTGIMLLLILEAHDRPHADAGGHFM
                     DMMFEAVSALGTVGLSTGVTADLVDSSRLVLVAMMLLGRVGPLAMASLLLRGPRGPKI
                     RYPESEVIVG"
     gene            complement(1610..1861)
                     /locus_tag="PLS34_08155"
     CDS             complement(1610..1861)
                     /locus_tag="PLS34_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87477.1"
                     /translation="MIDRKLVEILRCPVSRQGVTVAPKDRLRALNAAIAAGELEHQDG
                     RKVEGELEEALLTADGALIYRVRDGIPIMLADEAIVAPD"
     gene            complement(1884..2696)
                     /locus_tag="PLS34_08160"
     CDS             complement(1884..2696)
                     /locus_tag="PLS34_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRP87478.1"
                     /translation="MEFLQALLEHEFLQRSLAGGLLASIGCGVVGSYVVVQRISLLAG
                     AIAHAVLGGMGMAWYYGANPLAGALVAAVIAALIIGWVKLRFAEREDTAISAIWALGM
                     AVGILFIARTPGYSVDLMGYLFGNLLMMSSSDLQLIAALDIVILLLVALFFRQFLAVC
                     FDPEFARVRGLNVERYYLLLLVLVALTVVMLIQVVGLILVIALMTLPAAIAGRFTQSL
                     AAMMGLAVLLGMLFTTGGLALSYAPDLPSGPVIIVLAGLGYLLAAFVQRGAR"
     gene            complement(2696..3451)
                     /locus_tag="PLS34_08165"
     CDS             complement(2696..3451)
                     /locus_tag="PLS34_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP87479.1"
                     /translation="MNAALTFEGVSFGYGDGPNVVEDVDLEIGSHELIGLIGPNGGGK
                     STLLKLAAGLLRPRKGKVRVFGRPPHEARDEIGYVPQFAHFARDFPITVAQAVLLGRL
                     SGGFALRWSTADRAAAARAIEETELDALADRPLTALSGGELQRVLIARALAAEPRLLL
                     LDEPTSNLDQRAEEDIFALLARLNDRMAIVLVSHDVGFVTDFVQRVACISRTLICHTT
                     SEVTGEVINELYGHPVRAVHHAHHHHDTLRAKD"
     gene            complement(3448..4359)
                     /locus_tag="PLS34_08170"
     CDS             complement(3448..4359)
                     /locus_tag="PLS34_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRP87480.1"
                     /translation="MKAPKLLAAWLPALLLLSGPAAWGQAAPLQVVVSILPQQFLVER
                     IAGPRVEVLTLVQPGDNEATYSPGPATLAALDGARVWFTLGVHFENAWLGRITRDRPG
                     MQVVSLADGLPMRSTEQSLVAAGAADEHGHRHGGELDPHTWTDPRLAARMAGRIAETL
                     ARLDPGGADEYRRRAVRLQDELLALHEEIAARLAPVRGGAFIVFHPSWGYFADAYGLV
                     QLPIEVGGREPGPRGLAEIIRRGREAGVRAVFVQQQFSQRSARAVAEALGVEVVEADP
                     LAPDYVANLRQVSLRIAKALAAPGAGS"
     gene            4438..5268
                     /gene="ttcA"
                     /locus_tag="PLS34_08175"
     CDS             4438..5268
                     /gene="ttcA"
                     /locus_tag="PLS34_08175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRP87481.1"
                     /translation="MNAQPAPALSATARRLESRLRTQVGKAIEDYGMIADGDRVMVCL
                     SGGKDSYAMLSLLLALQRAAPVSFELVAVNLDQKQPGFPAHVLPDYLRSLGVPFHVLE
                     QDTYSVVKRVIPIGKTTCGLCSRLRRGVLYNFAAEQGFNKIALGHHLDDIMETLFLNL
                     FFGGTLKAMPPKLKSDDGRNVVIRPLAYCRESELARYATARAFPLIPCNLCGSQENLQ
                     RKEIKAMLADWERRFPGRIESIAAALGNVAPSQLMDAGLFDFARLAVEPAGGSGAKTP
                     "
     gene            complement(5341..5604)
                     /locus_tag="PLS34_08180"
     CDS             complement(5341..5604)
                     /locus_tag="PLS34_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRP87482.1"
                     /translation="MKSIYVGNLPFSATEDEVRGLFAQFGDVQSVKIVSDRETGRPRG
                     FGFVEMGDADADNAIKALEGHQMGGRALKINEARPKAPRPPRF"
     gene            complement(5770..6306)
                     /locus_tag="PLS34_08185"
     CDS             complement(5770..6306)
                     /locus_tag="PLS34_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRP87483.1"
                     /translation="MRRIAPWLLLAAVTALLGGCGGKHPPLPTVERVDLDRYLGTWYE
                     IARYPAPFQEGCVATSANYSLRDGGGIRVLNRCLVERFDGELREAEGRAKIVDRDTNA
                     RLKVTFFWPFYGDYWVLGLGPDYEWALVGEPSRKYLWILSRSRQMDPAVYAEIVARLP
                     ALGYDPERLLVTPQPPTD"
BASE COUNT         1057 a         2266 c         2200 g          850 t
ORIGIN      
        1 atgaacaccg cggcggcgcc ggccacgtcg agcgtcttga gtacttcctc gtcggcgccg
       61 tccgcgacca gcgcgctgct gagcgcgcct tcgacccgtt gcacggccgc ctcgtcgatg
      121 tcgaggcccg tgaccctgtg ctcgtgctct gcgagccgcc gcgcgagcgc cgcgccgaat
      181 cggcccagcc cgatgacgat gaacttcgaa cgcttgtcag ccaacgatca cctcgctttc
      241 cggataccgg atcttcggcc cccgcgggcc gcgcagcagc agcgacgcca tggccagcgg
      301 ccccacccgc ccgagcagca tcatcgccac cagcaccagg cgcgacgaat cgacgaggtc
      361 cgcggtgacg ccggtggaca gtccgaccgt gccgagcgcc gacaccgcct cgaacatcat
      421 gtccatgaaa tgcccgcccg cgtccgcatg cggtcgatca tgcgcctcga ggatcagcag
      481 cagcatgatc cccgtcaaca gcaccagcgc gtagaccacc acgaccgcgg ccgccgtggc
      541 gagcacccga gtgggaatgc cgtgccggaa ggccgccgcc tgccagcggc ggcgcagtag
      601 cgacacccct tccaccgtga gcaccgccac ggtggacacc ttgacgccgc cggcggtcga
      661 gcaggcgccg ccgccgatga acatcaacaa caacagcagg aacagggtcg ggtaggcgaa
      721 atgctccacc ggcagggtgg tgaagccggc ggtgcgcgcc gtcaccccct ggaaaaaagc
      781 attcatcagt gccgttccgg cgggcaatcc cgccagcgcg ccatgccgct cggccagcca
      841 gatggcgagc gtgccgccag ccagcagcac aagcgtcgcg gtgatcacca gctggctgtg
      901 aaagcgcagc ccgcgcctgt cgccgccgcg ccgccggaaa tgaagcatgt cgagcagcac
      961 cgggaagccc agtccgccca ggatgatcag cgccgacatg gtgaggatga ttgccgggtc
     1021 gccggcccag ggcgcgagac tggcgtcctg cagcgcgata ccggaattgc agaagccgga
     1081 cacagcgtgg aacaccgccc accacaggat gtcgaggaac ggcccgtcgc cgagccgcgc
     1141 cagcagcagc gccgccgcgc cgacggcctc cacggcgagt gagacggcca gcaccagcag
     1201 gatcagccgg ccgatgttgg cgccgagcgg cgcgccgagg gattcgctgg tgagcaggcg
     1261 gtactggacc ggctggcggc cggtcaggct gacgaacagc agcgtgccga tggacatgat
     1321 gccgaggccg ccgagctgga tcagcagcag caccacgagc tgtccggtga agctcaggtc
     1381 ggtggccatg gagcgcacca tcagcccggt gacagaagcc gcgctggtcg cggtgaacag
     1441 cgcgtcgctg aagtccagcg gatccgcgcc gaccgcgtgg cagaccggca gcaacagcag
     1501 cgccgtgccg accgccacca ggccgaggta ccaggcgaac agcatccggg ccgggtaacc
     1561 cgccagggcg ccgacgaact tgaccaggcc ctgttgcacg cgctcggtcc tagtcggggg
     1621 cgacgatcgc ctcgtcggcc agcatgatcg gaatgccgtc gcgcacccgg tagatcagcg
     1681 cgccgtcggc cgtgagcagc gcttcctcga gctcgccttc gaccttgcgg ccgtcctggt
     1741 gctcgagttc gcccgcggcg attgcggcgt tcagcgcccg cagccggtcc ttcggcgcca
     1801 cggtgacgcc ctgccgactc accgggcagc gcaggatttc caccagcttt ctatcgatca
     1861 ttgaatcagt tccttgtgtc agctcagcgc gcgccgcgct gcacgaatgc ggccagcagg
     1921 tagccgaggc ccgccagcac aatgatgacc ggcccggagg gcaggtccgg cgcgtaggag
     1981 agcgccaggc cgccggtcgt gaacagcatg ccgagcagca ccgcgaggcc catcatggcg
     2041 gccagcgact gggtgaagcg cccggcgatc gccgccggca gggtcatcag cgcgatcacc
     2101 aggatcaggc cgaccacctg gatcagcatc accaccgtca acgccaccag caccagcagc
     2161 agcaggtaat agcgctccac gttgaggccg cgcacccgcg cgaactccgg gtcgaagcac
     2221 accgccagga actggcggaa gaacagcgcg accagcagca ggatgacgat gtcgagtgcc
     2281 gcgatcagct gcaggtcgct ggacgacatc atcagcaggt tgccgaacag gtagcccatc
     2341 aaatcgacgc tgtaacccgg ggtcctggcg atgaacagga tgccgacggc catgccgagc
     2401 gcccagatgg ccgagatggc ggtgtcctcg cgctccgcga agcggagctt cacccagccg
     2461 atgatcagcg cggcgatgac ggccgccacc agcgcgcccg ccagcgggtt ggcgccgtaa
     2521 taccaggcca tgcccatgcc gccgagcacg gcgtgcgcaa tggcgccggc cagcaggctg
     2581 atccgctgca ccaccacgta gctgccgacc acgccgcagc cgatgctcgc cagcagcccg
     2641 ccggccagcg agcgctgcag gaattcgtgc tccagcaggg cctgcaggaa ttccatcagt
     2701 ccttcgcccg cagcgtatcg tggtgatggt gcgcatggtg gaccgcgcgc accgggtggc
     2761 cgtacagctc gttgatgact tcgccggtca cctcgctggt ggtatggcag atcagcgtgc
     2821 ggctgatgca ggcgacgcgc tgcacgaagt cggtgacgaa gccgacgtcg tgcgacacca
     2881 gcacgatggc catgcggtcg ttcaatcgcg ccagcagcgc gaaaatatcc tcctcggcgc
     2941 gctggtccag gttggaagtc ggctcgtcga gcagcagcag ccgcggctcc gccgccagcg
     3001 cgcgcgcgat cagcacccgc tgcagctcgc cacccgacag ggcggtcagc ggccggtccg
     3061 ccagcgcgtc cagctcggtt tcctcgatgg cgcgcgctgc tgccgcccgg tcggccgtgc
     3121 tccagcggag cgcaaagccg ccgctcagcc ggccgagcag caccgcctgg gccaccgtga
     3181 tggggaaatc gcgcgcaaag tgcgcgaact gcggtacgta gccgatctcg tcgcgcgcct
     3241 cgtgcggcgg gcggccgaac acccgcacct tgcccttgcg cggcctgagc agcccggccg
     3301 cgagcttgag cagcgtgctc ttgccgccgc cgttcggccc gatcagcccg atcagctcgt
     3361 gcgagccgat ctcgaggtcg acgtcctcga ccacgttcgg cccgtcgccg tagccgaagc
     3421 tcacgccctc gaaagtcagc gcggcgttca tgaaccggcg ccgggcgcgg cgagcgcctt
     3481 ggcgatgcgc agcgagacct gccgcaggtt ggcgacgtag tccggcgcca gggggtcggc
     3541 ctcgaccacc tcgacgccga gtgcctcggc gacggcccgg gcgctgcgct ggctgaactg
     3601 ctgctgcacg aacacggccc ggacgccggc ctcgcggccg cggcggatga tctccgcgag
     3661 gccgcgcggg ccgggctcgc gcccgcccac ctcgatgggc agctgcacca gcccgtacgc
     3721 atctgcgaag tagccccagg acgggtggaa cacgatgaac gcgccgccgc gcaccggcgc
     3781 gaggcgcgcc gcgatctcct cgtgcagcgc cagcagctcg tcctgcagcc tcacggcgcg
     3841 gcgccggtat tcgtccgcgc cgccggggtc gagccgcgcc agggtctcgg cgatacgccc
     3901 cgccatccgc gcggcgagcc gcgggtcggt ccaggtgtgg ggatcgagct caccgccgtg
     3961 acggtgaccg tgctcgtccg ctgcgccggc cgcgaccagg ctctgctcgg tgctgcgcat
     4021 cggcaggccg tcggccagcg acaccacctg catgccgggg cggtcgcggg tgatgcggcc
     4081 cagccaggcg ttctcgaagt gcacgcccag ggtgaaccag acgcgcgcgc cgtcgagcgc
     4141 cgccagcgtg gcggggccgg gggaataggt ggcctcgttg tcgccgggct gcacaagggt
     4201 caggacctcg acgcgcgggc cggcgatgcg ttcgaccagg aactgctgcg gcaggatgct
     4261 gaccaccact tggagcggcg ccgcctgccc ccaggccgcc ggcccggata gcagcagcag
     4321 ggccgggagc caggccgcca ggagcttggg cgctttcatc cggctattct accgcgccgg
     4381 tcaagccaat cagggcattt gcattgggtt agaatgccgg gccccggagg agacccgatg
     4441 aacgcccagc ccgcgcccgc cctgtccgcc accgcccgcc gcctcgagtc gcggctgcgc
     4501 acccaggtgg gcaaggccat cgaggactac ggcatgatcg ccgacggcga tcgggtcatg
     4561 gtctgcctgt ccggcggcaa ggacagctac gcgatgctga gcctgttgct ggcgctgcag
     4621 cgggccgcgc cggtgtcctt cgagctggtg gcggtcaacc tcgaccagaa gcagccgggg
     4681 ttcccggcgc acgtgctgcc ggattacctg cgctcgctgg gcgtgccctt tcacgtcctg
     4741 gagcaggaca cctacagcgt ggtgaagcgc gttattccca tcggcaagac cacctgcggc
     4801 ttgtgctcgc ggctgcggcg cggcgtgctc tacaacttcg cggcggaaca gggcttcaac
     4861 aagatcgcgc tggggcacca cctcgacgac atcatggaga cgctgttcct caacttgttc
     4921 ttcggcggca cgctgaaggc catgccgccc aagctgaaga gcgacgacgg gcgcaacgtg
     4981 gttatccggc cgctggccta ttgccgcgaa agcgagctgg cgcgttacgc caccgccagg
     5041 gccttcccgc tgattccctg caacctgtgc ggcagccagg agaacctgca gcgcaaggag
     5101 atcaaggcga tgctggccga ctgggagcgg cgcttcccgg ggcgcatcga gtcgatcgcc
     5161 gccgcgctcg gcaacgtggc gccgtcgcag ctgatggatg cgggactgtt cgatttcgcc
     5221 cggctggcgg tggagccggc gggcgggtcg ggcgcaaaaa caccctgagg ccgcgcgccg
     5281 gcgcggcaag gcaggaggca ccagtcacgg gccgggcgcc gtgcccggcg cacgcttcgt
     5341 tcagaaccgc ggcgggcgcg gtgctttcgg acgagcctcg ttgatcttca gcgcgcggcc
     5401 gcccatctgg tggccttcga gggccttgat ggcgttgtcg gcgtccgcat cgcccatttc
     5461 gacgaacccg aaaccacgcg gccggccggt ctcgcgatcg ctcacgatct tgaccgactg
     5521 gacgtcgccg aactgggcga acaggccgcg aacttcatct tcggtggcgg agaagggcag
     5581 gttgcccacg tagattgact tcatctcaag aggctctcag gaacggaaca cgatcacggg
     5641 cgccgccacc cgacggcgcc gccacagcat acgacaggcg gcgaagtgcg caaagtggcg
     5701 cccgagcggg gcgcaccggg ccccggtccc gcatccgcgc aagcatcgga ccggacgcgg
     5761 ccaggagggc tagtccgttg gcggctgggg cgtgacgagc agccgctccg ggtcataacc
     5821 aagcgccggc aggcgggcga cgatctccgc gtacaccgcc gggtccatct gccgactgcg
     5881 tgacaggatc cacaggtact tgcgtgacgg ctcgccgacc agcgcccact cgtagtccgg
     5941 accgaggccg agcacccagt agtcgccgta gaagggccag aagaaggtca ccttcaacct
     6001 ggcgttggtg tcccggtcga cgatcttcgc gcgcccctcg gcctcgcgca gctcgccgtc
     6061 gaagcgctcc accaggcagc ggttgagcac ccggattccg ccgccgtcgc gcaggctgta
     6121 gttcgcgctg gtcgccacgc agccctcctg gaagggcgcc ggatagcggg cgatctcgta
     6181 ccaggtaccg aggtagcgat cgagatcgac gcgctcgacg gtgggcaggg gcgggtgctt
     6241 gccgccgcaa ccgccaagca gggcggtaac ggcggcgagg agaagccagg gggcgatgcg
     6301 gcgcatggcg aggtccgggt ctgaatcgat gccgctagcg tacctccgcg gcccgtgctt
     6361 cacacgcgcc ggg
//
LOCUS       DAOMPG010000135         6321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5251_length_6321_cov_4.175870, whole genome shotgun sequence.
ACCESSION   DAOMPG010000135 DAOMPG010000000
VERSION     DAOMPG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5251_length_6321_cov_4.175870"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLS34_08190"
     CDS             <1..370
                     /locus_tag="PLS34_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRP87484.1"
                     /translation="NGSQAQLEFLPERSTDFIFAALGEEFGLLGLVFLIAAYLFLVGR
                     GMYIALQAQDTYTRLLAGSISLTFFVYVFVNTGMVTGLLPVVGVPLPLVSYGGTSMVT
                     LMAGFGILMAINGHRNLLAR"
     gene            367..1389
                     /gene="mltB"
                     /locus_tag="PLS34_08195"
     CDS             367..1389
                     /gene="mltB"
                     /locus_tag="PLS34_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HRP87485.1"
                     /translation="MKRFNPLLGCAALAAAQFATTAAPAAIELDLERADVRDFIARVE
                     REQQLDAETVRSILGLARVQPRIIEAISRPAERVRPWHEYRKIFMTEERISAGVEFWQ
                     THRERIEEVAASTGVPVEILVGIIGVETFYGRIIGRYRVLDALATLAFEYPPRSAFFA
                     RELEQFLLLAREQGLEIEAPLGSYAGAMGLPQFIPSSYRAYAVDGDGDGRIDLWDSPD
                     DIFASVANYFKAHGWRPGEPVVAPAVPGDGDPSGLAQQGLQADITVGALWDAGVGLAG
                     PAPRDRRAAAGLFVLEHEEGPRYWAGFHNLYVITRYNRSLMYALAVHQLGEAIAARMA
                     EAADAP"
     gene            1379..2209
                     /locus_tag="PLS34_08200"
     CDS             1379..2209
                     /locus_tag="PLS34_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRP87486.1"
                     /translation="MRLDRGWHSRATALLAALAVVAGCTPARDGPPAVDVDGAPARPP
                     DVTRIPDPVPRAEPRSARGNPPFYEVMGRRYYVMKEAAGYRERGIASWYGTKFHGRTT
                     SSGEPYDMYAMTAAHKTLPLPSYVRVTHRGNGRSIIVRVNDRGPFVGDRIIDLSYAAA
                     VRLGMHNEGTALVDVELIEPDGPPPVVASVSPEMPPARAETVWLQVGAFSDERNAARL
                     RERLAGKGINDVVLSTEQRQRHPVYRVRIGPLSAVEDVERMLERVRELGIRDAHLAMD
                     "
     gene            2285..3442
                     /locus_tag="PLS34_08205"
     CDS             2285..3442
                     /locus_tag="PLS34_08205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRP87487.1"
                     /translation="MRLTFLNLLAFALLAASPVAFSQQPAAALPPVPAAPAVAARGYI
                     LVDFNSGEVLAEKDADTPMDPASITKLMTAYAVFSELRKGHMALDDMVTVSERAWRTG
                     GSRMFIEVGTRVSVEDLIRGMIIQSGNDASVALAEHTAGTEAAFAELMTEYALRLGMS
                     NTRFRNATGLPAEDHYSSARDIAILAGAIIREYPEFYQFYSEREFTYNNITQRNRNQM
                     LWRDPSVDGMKTGMTDGAGYCLVSSAEREGMRLVSVLLGTASAKARADATQALLNWGF
                     RFYESHRLYAAGEPVTTARLWMGEAEQLPVGLREDLWVTVPRGRYDLMQASMELQSPL
                     TAPVTTDDVIGRLRVSLEGRELADRSLHPLQGAPQAGFLRRAIHSVLLWFE"
     gene            3439..3699
                     /locus_tag="PLS34_08210"
     CDS             3439..3699
                     /locus_tag="PLS34_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HRP87488.1"
                     /translation="MSDSLLEFPCDFPIKVMGRKEAGFSEVVVALVEAHTGPIAAERV
                     QLRESRDGNFVSLTLMVRTESQAQLDAIYSSLSGHDQVLMVL"
     gene            3699..4337
                     /gene="lipB"
                     /locus_tag="PLS34_08215"
     CDS             3699..4337
                     /gene="lipB"
                     /locus_tag="PLS34_08215"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRP87489.1"
                     /translation="MQAPVVRRLGRVEYEPTWRKMQAMTEARGADQPDELWFLQHPPV
                     FTLGLNGRREHLLAPGDIPVVQVDRGGQVTYHGPGQLVVYPLLDLGRRGLGVRQLVCA
                     LERAVIRCVASYGIEAVGDRDAPGVYVAGRKLASIGLRIRRNCCYHGVALNVAMDLVP
                     FQRINPCGFQGLEVTQLADLGAKHPMERVAADLEAALLAELAQASPSAACSA"
     gene            complement(4304..4969)
                     /locus_tag="PLS34_08220"
     CDS             complement(4304..4969)
                     /locus_tag="PLS34_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRP87490.1"
                     /translation="MKAMILAAGRGERMRPLTDRMPKPLLPVGGEPLVAHHLKALAAA
                     GCREVVMNCSWLADQLRAALGDGRAWGVSIEYSPEPWPPLDTGGGIFAALPMLGPGPF
                     LLLNGDVWCDLSPASLELPAGMLGHLVLVPNPPHNAQGDFGLADGRVDPDATPRLTYG
                     GMAILTPELFVGCRAGRFPLAPILAAAARQGKIGGMLHQGVWHDVGTPERLYALQAAL
                     GEA"
     gene            5059..>6321
                     /gene="lptD"
                     /locus_tag="PLS34_08225"
     CDS             5059..>6321
                     /gene="lptD"
                     /locus_tag="PLS34_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRP87491.1"
                     /translation="MSRFLALSLALMMLTTGAAAREYAACPAPEVIDFGPIDESGRIE
                     VSARNADVSAQGDSEFSGGVIVQRGAQRLETESATWDRQAGRVTALHGARYLQPGFSV
                     EADVLEFRPAEGAAILTGSRYRLPAQPASGGAERIEARDSGRVTLDGVTYTTCPGDDP
                     DWLLKIDRLKLDIDREVGEARQVTLRLFDTPLIYWPYLSFPLSDRRKSGFLVPKFGQS
                     TRSGFEFALPYYWNLAPNYDLMVTPTFLSSRGLQLRNEYRYLTTRSSGDLAVDYLPRD
                     DARAETDSRSWMNLAHLTRFENGWRVAAQLEDVSDTDYFQDLGNTPQVTSRTHVERLL
                     QADYAGRVWRITGRMQNFRTLDLTIPEDERPYARLPQVIASAQLPDGLLGLNWRLRTE
                     AGSFTRDVGPEGVRVVMEPGMSLPLEAPG"
BASE COUNT          988 a         2184 c         2157 g          992 t
ORIGIN      
        1 caacggctcg caggcgcagc tggaattcct gcccgagcgc tcgaccgact tcatattcgc
       61 ggcgcttggc gaggagttcg ggctgctcgg actggtcttc ctgatcgcgg cttacctgtt
      121 cctggtgggt cgcgggatgt acatcgccct gcaggcgcag gacacctaca cgcgcctgct
      181 ggccggcagc atcagcctga ccttcttcgt gtacgtgttc gtcaacaccg gcatggtcac
      241 cggcctgctg ccggtggtcg gcgtgccgct gccgctggtg agctacgggg gcacctcgat
      301 ggtgaccctg atggcgggtt tcggtattct catggccatc aacggccaca gaaacctact
      361 cgccaggtga agcgcttcaa tcccctgctc ggctgcgcag ccctggccgc cgcgcaattt
      421 gccaccacgg ccgcgcccgc cgccatcgag ctcgacctcg aacgcgccga cgtgcgcgat
      481 ttcatcgcgc gggtcgagcg cgaacaacag ctggacgctg aaaccgtgcg ctcgattctc
      541 gggctcgcgc gcgtccagcc gcggatcatc gaggccatca gccggcctgc cgaacgtgtc
      601 cgcccgtggc acgagtaccg caagatcttc atgaccgagg agcggatcag cgccggcgtg
      661 gagttctggc agacgcaccg cgaacgcatc gaagaggtgg ccgcaagcac cggtgtgccg
      721 gtcgaaattc tcgtcggcat catcggcgtc gagacttttt acggacggat catcggccgc
      781 taccgggtgc tcgatgcgct tgccacactg gccttcgagt acccgccgcg cagcgctttc
      841 ttcgcccgcg agctggagca gtttctgctg ctggcgcgcg agcaggggct cgagatcgag
      901 gcgccgctcg gctcctacgc cggcgccatg ggcctgccgc agttcatccc gtcgagttac
      961 cgcgcctatg cggtcgacgg cgacggcgac ggccgcatcg acctgtggga cagcccggac
     1021 gacatcttcg cgagcgttgc caactacttc aaggcgcacg gctggcgtcc cggggaaccc
     1081 gtggtggcgc cggcggtgcc cggggacggc gacccgtcgg gcctcgctca gcagggtctg
     1141 caggccgaca tcacggtggg cgcgctgtgg gacgccgggg tgggtctggc cgggccggcg
     1201 ccgcgcgatc gtcgcgccgc tgccgggctg ttcgtactcg agcacgagga agggccgcgt
     1261 tactgggcag gctttcacaa cctgtacgtg atcacccgct acaaccgcag cctgatgtac
     1321 gcgctcgccg tccaccagct cggtgaagcg atcgcggcgc gcatggcgga ggccgcggat
     1381 gcgccttgat cgaggttggc actcgcgcgc gacagcgctg ctggccgcgc ttgccgtagt
     1441 cgcgggctgc acgcctgcgc gggacggtcc gcccgccgtc gatgtcgacg gcgcccccgc
     1501 gaggccgccg gacgtcacgc ggatccccga ccccgtgccg cgtgccgagc cgcgcagcgc
     1561 acgcggcaat ccgcccttct acgaggtaat gggccgtcgc tactacgtga tgaaggaggc
     1621 cgccggctac cgcgagcgcg gcatcgcgag ctggtacggc accaagttcc acggccgcac
     1681 gacttccagc ggcgagccct acgacatgta cgccatgacg gccgcacaca agactttgcc
     1741 gttgcccagt tacgtgcggg tcacgcaccg cggcaacggc cgcagcatca tcgtccgcgt
     1801 caacgaccgc ggacccttcg tcggcgaccg catcatcgac ctgtcctacg cggctgccgt
     1861 gcgcctcggc atgcacaacg aggggaccgc cctggtcgac gtcgagctca tcgaacccga
     1921 cgggccgccg ccggtcgtcg ccagcgtctc tcccgaaatg ccgccggccc gcgccgagac
     1981 cgtctggctg caggtaggcg ccttctcgga cgaaaggaac gcggcgcgac tgcgggaacg
     2041 actcgccggc aagggcatca acgatgtggt cctgtcgacc gaacaacgcc agcggcaccc
     2101 cgtttaccgt gtcaggatcg ggccgttgag cgcggtggag gacgtcgagc gcatgctgga
     2161 acgcgtgcgt gaactgggaa tcagggacgc ccacctggcg atggactgac gctcggtgag
     2221 cgagcgtgta ccatttcccg cctgttcact ttctttatgg cggccgcgcg ccgcgctggg
     2281 agccatgcgc ctgacattcc tgaatctgct tgccttcgcc ctgttggcag cgtcgccggt
     2341 ggcgttttct caacaacccg ccgccgcgct gccgccggtg cccgccgccc cggcggtcgc
     2401 ggcccgcggc tacatcctgg tcgacttcaa ttccggcgaa gtgctggcag aaaaggacgc
     2461 cgacacgccg atggacccgg ccagcatcac caagctgatg accgcctacg cggtgttcag
     2521 cgagctgcgc aagggccaca tggcgctcga cgacatggtg acggtcagcg agcgggcctg
     2581 gcggaccggc ggctcacgga tgttcatcga ggtcggcacc cgggtgagcg tcgaggattt
     2641 gatccgcggc atgatcatcc agtccggcaa cgacgcctcg gtggcgctcg ccgagcacac
     2701 ggccggcacg gaggccgcgt tcgccgagct gatgaccgag tacgctttgc gcctcggcat
     2761 gtccaacacg cggtttcgca acgccaccgg cctgccggcc gaagaccact acagctccgc
     2821 gcgggacatc gcgatcctgg cgggcgcgat catccgcgag tatcccgagt tctaccagtt
     2881 ctactcggag cgcgagttca cctacaacaa catcacccag cgcaaccgca accagatgtt
     2941 gtggcgcgac ccgtcggtgg acggcatgaa gaccggcatg acggacggtg ccggttactg
     3001 cctcgtgtcg tcggcggagc gcgagggcat gcggctggta tcggtgctgc tcgggacggc
     3061 cagcgccaag gcgcgcgccg acgccaccca ggccctgctg aactggggtt tccgcttcta
     3121 cgaaagccac cggctgtatg cggcgggtga accggtgacg acggcccggc tgtggatggg
     3181 cgaggcagag cagctgccag tgggcctgcg cgaagacttg tgggtcacgg tgccgcgcgg
     3241 ccgttacgac ctcatgcagg cctcgatgga actgcaatcg ccgctgaccg ccccggtcac
     3301 gaccgacgac gtgatcgggc gcctgcgggt cagcctcgag ggtcgtgagc tggcggatcg
     3361 cagcctgcat ccgttgcagg gggcaccgca ggccggcttc ctgcgacgcg ccatccattc
     3421 cgtgctgctg tggttcgaat gagcgacagc ctgctggagt tcccctgtga ctttcccatc
     3481 aaggtcatgg gccggaagga agccggcttc agcgaggtcg tggtggcgct ggtcgaggcc
     3541 cacacgggac cgattgcggc ggagcgcgtg cagctgcgcg agagccgcga cggcaacttc
     3601 gtgtccctga cgctgatggt gcgcacggaa agccaggccc agctcgacgc gatttacagc
     3661 tcgctgtcag gccacgacca ggtcctgatg gtgctgtgat gcaggcaccc gtcgtccggc
     3721 ggctcggccg cgtcgagtac gagccgacct ggcggaagat gcaggccatg accgaggcgc
     3781 gtggcgcgga ccagccggac gagctgtggt tcctccagca cccgcccgta tttaccctcg
     3841 gcttgaacgg acggcgggag cacctgctgg cgccgggcga catcccggtg gtgcaagtgg
     3901 atcgcggcgg ccaggtgacc taccacgggc ccggccagct ggtggtctat cccctgctgg
     3961 atctcggccg ccgcggattg ggggtgcgcc agctggtgtg cgcgctggag cgcgccgtta
     4021 tccgttgcgt ggcaagctac ggcatcgagg ccgtcggcga ccgcgacgcc cccggcgttt
     4081 acgtcgcggg ccgcaagctg gccagcatcg gcctgcgaat ccggcgcaac tgctgttacc
     4141 acggggtggc gctgaacgtg gccatggacc tggtgccgtt ccagcggatc aatccctgcg
     4201 gcttccaggg gctcgaggtg acgcaactcg cggaccttgg cgcgaaacac ccgatggaac
     4261 gtgttgccgc ggacctggaa gcggcactgc ttgccgaact ggctcaggcc tcgcccagcg
     4321 ctgcctgcag cgcatagagc cgctcgggcg ttcccacgtc gtgccagacg ccttggtgca
     4381 gcatgccgcc gattttgccc tggcgggccg ccgccgccag gatcggcgcc agcggaaagc
     4441 gccccgctcg acagcccacg aacaactcgg gcgtaaggat cgccatgccg ccgtaggtca
     4501 agcgcggcgt cgcgtccgga tcgacgcggc cgtcggccag gccgaaatca ccttgtgcgt
     4561 tgtggggcgg gttgggcacg agcaccaggt ggccgagcat cccggcgggg agctcaagcg
     4621 acgcgggcga gagatcgcac cagacgtccc cgttgagcag caggaaaggg cccgggccga
     4681 gcatcggcag ggccgcgaaa atcccgccgc cggtgtccag cggcggccag ggctccgggg
     4741 agtactcgat ggacacgccc catgcacggc cgtcgccgag cgctgcgcgc agctggtccg
     4801 ccagccagga gcagttcatg acgacctccc ggcaaccggc cgccgccagg gccttgaggt
     4861 gatgcgccac caacggctcc ccgccgaccg gcagcagcgg cttgggcatt cgatccgtca
     4921 gggggcgcat gcgctcgccg cggccggccg ccaggatcat cgctttcact ggatctcccc
     4981 gtccgggccc gccgcgggcc atgattgatg atgagcgtca aagtatgcca tgttagattt
     5041 cggccgccgg gaagccagat gtctcgcttt ctcgccctgt cacttgcctt gatgatgttg
     5101 accaccggcg cggccgcccg cgagtacgcg gcgtgcccgg cgcccgaggt gatcgacttc
     5161 ggcccgatcg acgagagcgg gcggatcgaa gtgtcggcgc gcaacgcgga cgtcagtgcc
     5221 cagggcgact ccgagttctc cggcggggtc atcgtgcagc ggggcgccca gcgcctcgaa
     5281 accgaatccg ccacctggga ccggcaggcg gggcgggtga ccgccctgca cggagcgcgc
     5341 tacctccagc ccggcttcag cgtcgaggcc gacgtgctcg agtttcgtcc cgcagaaggc
     5401 gccgcaattc tgactggcag ccgttatcgc ctgccggcgc agcccgccag cggcggcgcc
     5461 gagcgcatcg aggcgcgcga cagcgggcgt gtcacgctgg acggcgtgac ttacaccacc
     5521 tgccccggcg acgaccccga ctggctgctg aagatcgatc gcctgaaact ggacatcgat
     5581 cgggaggtcg gcgaggcgcg ccaggtcacg ctgcggctgt tcgacacgcc gttgatctac
     5641 tggccctacc tgtcctttcc gctgtccgac cgccgcaaga gcggttttct ggtgccgaag
     5701 ttcggccagt cgacgcgcag cggcttcgag ttcgccctgc cctactactg gaacctcgca
     5761 ccgaactacg acctcatggt gacgccgacc ttcctgtcga gccgtggcct tcagctcagg
     5821 aacgaatacc gctacctgac cacgcgcagc agcggagacc ttgccgtcga ctacctgccc
     5881 cgtgacgatg cccgggcgga gaccgactca cgaagctgga tgaacctcgc ccacctgacg
     5941 cgcttcgaga acgggtggcg cgtcgcggcc cagctcgagg acgtttccga cacggactat
     6001 ttccaggacc tcggcaacac gccccaggtc accagccgca cgcacgtcga acgcctgctg
     6061 caggccgatt acgcaggccg ggtctggcgg atcacgggtc gcatgcagaa cttccgcacg
     6121 ctcgacctga ccatccccga ggacgagcgg ccctatgcgc gattgcccca ggtgatcgcc
     6181 agcgcgcagc tgcctgatgg cctgctggga ttgaactggc ggctgcgcac cgaggccggc
     6241 agcttcaccc gcgatgtggg gcccgaggga gtgcgcgtgg tcatggagcc gggaatgagc
     6301 ctgccgctgg aggcgccggg c
//
LOCUS       DAOMPG010000136         6315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5257_length_6315_cov_3.605272, whole genome shotgun sequence.
ACCESSION   DAOMPG010000136 DAOMPG010000000
VERSION     DAOMPG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_6315_cov_3.605272"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="ribB"
                     /locus_tag="PLS34_08230"
     CDS             <1..1009
                     /gene="ribB"
                     /locus_tag="PLS34_08230"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRP87492.1"
                     /translation="AGSMVRAEDVNFMARYGRGLICLTLTPERCEQLRLPLMVTGTDR
                     ARSTNFTLSIEAAEGVTTGISAHDRARTIRAAVAPNARPEDLRQPGHIFPLMGQPGGV
                     LTRAGHTEAGCDLARLAGLEPAAVIVEILNEDGTMARRPDLVSFARSHGLRMCTIAQL
                     IRYRLEKEESVEQISQDRIDTEFGPFRLVCFEDHVHQDVHLALIRGDIGPGAVPLVRV
                     QLTDTLRDLVGVQAPQLGWPLRNAMQRIAQEKAGIVVILRRHELPRELVDSVSLLSGR
                     PLAPGDHRDGQESRVLRTYGIGAQILRSLGVRRMRVLSAPKQMFGLSGFGLEVVEYVN
                     Q"
     gene            1035..1511
                     /gene="ribE"
                     /locus_tag="PLS34_08235"
     CDS             1035..1511
                     /gene="ribE"
                     /locus_tag="PLS34_08235"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014149346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRP87493.1"
                     /translation="MDKVKKIEAELSARDLRIGIVAARFNDFIVDSLLRGAVDTLLRH
                     GASEKDITLVRVPGAWEMPWAVKKMAAGKRYDAIIALGAVIRGATPHFDYVAGECVKG
                     VAQAGLAAEVPVAFGVLTTDTIEQALERAGTKAGNKGADAAMAAIEMARLLPRLEA"
     gene            1514..1966
                     /gene="nusB"
                     /locus_tag="PLS34_08240"
     CDS             1514..1966
                     /gene="nusB"
                     /locus_tag="PLS34_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRP87494.1"
                     /translation="MAEPGHRRARRGRSASRELALQALYQWQIGGQGGQDVLLEFLAE
                     RMTPGADEDYFKELVTQATGRSAELDQALGEFSDRPVAQLDPVEHGILLIGLYELAHR
                     PEVPWRVVINEAVDLCKRYGGSDGHRFVNALLDRSARRYRALETGTAG"
     gene            1983..2537
                     /locus_tag="PLS34_08245"
     CDS             1983..2537
                     /locus_tag="PLS34_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRP87495.1"
                     /translation="MPLPEPAEREAIHRRDIECRGFRRADGLWDIEGRMTDVKAYGFD
                     SRYRGRLEPGMPVHDMHMRMTLDDRLVVRDLQVASDAHPFPNCAEIESAYATLVGTML
                     KPGWTSLVRERLGGVAGCTHLTRLAQELAVVAMQTIMPLRSRDEAVTEGQERKPPHLD
                     GCHALATDGPIVREIAPRWYRGEQ"
     gene            2534..3487
                     /gene="thiL"
                     /locus_tag="PLS34_08250"
     CDS             2534..3487
                     /gene="thiL"
                     /locus_tag="PLS34_08250"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRP87496.1"
                     /translation="MSGGEFGLIARLVAATPCRRGDVLVGPGDDGAVLRAPPGMDLVQ
                     TIDTCLEGVHFPPGLDAADIGWRSLAVNLSDLAAMGAEPAWGLLSLSLPRADEGWLDG
                     FASGVAALASESGIDIVGGDTVRGPLAVTFALTGFVPPGAALRRNGARPGDGIWVTGP
                     LGGGAAGLAAWRRGGREDATGFLRPRPRLAEGRALRGMAGAAIDVSDGLLQDLGHVLE
                     ASGVGATVALERVPLAPGAAAAGEAAGLAMALDGGDDYQLCFAVPAARESELLAAAAG
                     WDALPVRIGAIRAEPGLVLTRDGQPVTRPAGGWDPFREVAP"
     gene            3484..3966
                     /locus_tag="PLS34_08255"
     CDS             3484..3966
                     /locus_tag="PLS34_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRP87497.1"
                     /translation="MSALSPRLLRDPVHLLALGFGTGLAPRAPGTAGTLVGVLLDPLL
                     RPLGLELRILVVALMFGAGVWLCGESARRLGVHDHPAIVWDEIVGYLALMLVLPAGWG
                     WALAGFVAFRFFDIVKPWPIRQLDHGVGGGLGIMVDDVMAAAWGAVVLLGIGFALGIQ
                     "
     gene            3963..5279
                     /locus_tag="PLS34_08260"
     CDS             3963..5279
                     /locus_tag="PLS34_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRP87498.1"
                     /translation="MSVEPKEMDASHPSKGEMPDKIGKYSIIREVGRGSSGTVYLSHD
                     PFYGRDVAIKLYKEPSGEDESELRVARKMFFNEAQMVGRLQHPNILPIYDAGEENGRY
                     YVVMEHVHGARTLSAYCRPDNLLPIEEVVRIIFNTVRAMHYAHTRGVVHRDIKPSNIM
                     LTMSNEVRVIDFGIALWVDNEVSVIRGVAGSPSYMSPEQIKAEEIGPRSDLYALGAVM
                     YELLTGRRPFKAANLAKLLHQIVFATPHPVHKLRPDVPEELEEVVWRAMRKKPEDRFS
                     NGLEFAAALTRIHQTLRSTRDALDERERVAILRGLRFFHDFSHTEIRELIKAAEWREY
                     APQQEIVRQGEMDDRFYVIISGEVAVEVDGQKVGHLMPGDCFGESSVVPNARRQATIR
                     ALGAVTLLQVSSTLLETLSAACQLRFNRMFLQTLIQRLQGSGHANA"
     assembly_gap    5566..5575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5694..6188
                     /locus_tag="PLS34_08265"
     CDS             5694..6188
                     /locus_tag="PLS34_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HRP87499.1"
                     /translation="MASEVQAMGLWRRLGGSELGRWLFTRIVCVKAPYFASISPRLTR
                     LEPGRCEAYLKHRRRVQNHIGTVHAIALCNLAELCAGLVTDVSIPRDMRWIPQDMTVR
                     YLKKAAGRISAIATPATELRSTAEPYPATIEVRLSDAAGQLVASADIHMWLSPRPGQQ
                     ARKA"
BASE COUNT          920 a         2187 c         2279 g          919 t
ORIGIN      
        1 ggcgggctca atggtgcggg ccgaggacgt caacttcatg gcccgctacg gacgcgggct
       61 gatctgcctg acgctcacgc cggagcgctg cgagcagctg cgcctgccgc tgatggtcac
      121 cggcaccgac cgcgcgcgca gcaccaattt cacgctgtcc atcgaggcgg ccgagggcgt
      181 gaccacgggc atctctgccc acgatcgcgc gcgcaccatc cgcgccgccg tggcgccgaa
      241 cgcgcgcccc gaggacctgc gccagcccgg ccacatcttc cccctgatgg gccagcccgg
      301 cggggtactg acgcgcgccg gccacaccga ggccggttgc gacctggcgc ggctcgccgg
      361 cctcgagcct gccgccgtga tcgtcgagat tctcaacgag gacggcacca tggcgcgccg
      421 tccggacctg gtgtctttcg cccgcagcca cggcttgcgg atgtgcacca tcgcccagct
      481 gatccgttac cggctggaga aagaggagtc ggtcgagcag atctcgcagg accggatcga
      541 caccgagttc gggccgttcc ggctggtgtg cttcgaggac cacgtccacc aggacgtgca
      601 tctcgcgctg atccgcggcg acatcgggcc gggcgccgtg ccgctggtgc gcgtgcagct
      661 gaccgacacc ttgcgcgacc tggtcggtgt gcaggcgccg cagctcggct ggccgttgcg
      721 caacgccatg cagcggatcg cgcaggaaaa ggccggcatc gtggtgatcc tgcgccggca
      781 cgagctgccg cgcgagctgg tggactcggt gtcgctgctg tccggccggc cgctggcgcc
      841 gggtgatcac cgcgacggcc aggagtcgcg cgtgctgcgc acctacggca tcggcgcgca
      901 gatcctgcgc agcctggggg tgcgcaggat gcgggtgctg tctgcgccca agcagatgtt
      961 cggcctctcg ggcttcggcc tcgaggtggt cgagtacgtc aaccagtgaa ccgccgccgc
     1021 gggggaatgc cgtcatggac aaagtgaaga agatcgaagc cgagctgtcc gcgcgcgacc
     1081 tgcgcatcgg catcgtggcg gcgcggttca acgacttcat cgtggacagc ctgctgcgcg
     1141 gtgcggtgga cacgctgctc aggcacggcg ccagcgagaa ggacatcacg ctggtgcggg
     1201 tgcccggcgc ctgggagatg ccctgggcgg tcaagaagat ggccgccggc aagcgctatg
     1261 acgccatcat cgcgctgggc gcggtgatcc gcggggcgac gccgcatttc gactacgtgg
     1321 caggcgagtg cgtcaagggc gtggcgcagg ccggcctggc ggccgaggtc ccggtcgcct
     1381 tcggcgtgct gaccaccgac accatcgaac aggcgctcga gcgcgccggc acgaaggccg
     1441 gcaacaaggg cgccgacgcc gccatggcgg ccatcgagat ggcgcgcctg ttgccgcggc
     1501 tcgaggcctg agcgtggcgg aacccggtca tcgtcgtgcg cgccgcggcc gcagcgcctc
     1561 gcgcgaactg gccctgcagg ccctgtacca gtggcagatc ggcgggcagg gcggccagga
     1621 cgtgctgctg gagtttctcg ccgagcgcat gacgcccggc gccgacgagg actatttcaa
     1681 ggaactcgtc acccaggcca ccgggcgcag cgccgagctc gaccaggcgc tgggcgagtt
     1741 ctccgaccgg cccgtggcgc agctcgaccc cgtcgagcac ggcatcctgc tgatcgggct
     1801 gtacgagctg gcgcatcgcc cggaagtgcc gtggcgggtg gtgatcaacg aggccgtgga
     1861 cctgtgcaag cgctacggcg gcagcgacgg ccatcgcttc gtcaacgcgc tgctggatcg
     1921 ctccgcgcgc cgttatcgcg ccctggaaac cggcacggcc ggctgagcca cgggagacgc
     1981 ccatgccatt gcccgagccc gcagaacgcg aagcgatcca tcggcgcgac atcgagtgcc
     2041 gcggcttccg ccgggccgac ggcctgtggg acatcgaggg ccgcatgacg gacgtcaagg
     2101 cctacggatt cgacagtcgc tatcgcggcc gcctcgagcc cggcatgccg gtgcacgaca
     2161 tgcacatgcg catgacgctg gacgaccggc tggtggtgcg cgacctgcag gtcgcctccg
     2221 acgcgcaccc cttcccgaac tgcgccgaga tcgagtcggc ctatgccacg ctggtcggca
     2281 cgatgctcaa gccgggctgg accagcctgg tgcgcgagcg gctgggcggt gtggcgggct
     2341 gcacgcatct cacgcgcctg gcacaagaac tcgcggtggt cgccatgcag accatcatgc
     2401 cgctgcgcag ccgcgacgag gcggtcactg agggccagga gcgcaagccg ccgcatctcg
     2461 acggctgtca cgcactggcg accgacgggc cgatcgtgcg cgagattgcg ccccggtggt
     2521 accggggcga gcagtgagcg gcggcgagtt cggtctgatc gcccggctgg tggccgcgac
     2581 tccctgccgg cgcggcgatg tgctggtcgg ccccggcgat gacggggcag tgctgcgtgc
     2641 gccgccgggc atggacctcg tgcagaccat cgacacctgt ctcgagggcg tgcatttccc
     2701 gccggggctc gatgccgccg acatcggctg gcgcagcctg gcggtgaatc tctccgacct
     2761 ggcggcgatg ggcgctgaac ccgcctgggg cctgctgtcg ctgtcgctgc cccgggcgga
     2821 cgagggctgg ctggacgggt tcgcgagcgg cgtcgcggcg ctggcctcgg agtcgggaat
     2881 cgacatcgtg ggcggcgaca ccgtgcgcgg gccgctcgcg gtcaccttcg cgctgaccgg
     2941 cttcgtcccg ccgggcgcgg cgctgcggcg caacggcgcc cggcccggcg acggcatctg
     3001 ggtgaccggc ccgctcggcg ggggcgctgc cggactggcc gcatggcgtc gcggcgggcg
     3061 cgaggacgcg accgggttcc tgcggccgcg tccgcggctg gccgagggcc gggcgctgcg
     3121 cggcatggcc ggtgccgcca tcgacgtctc cgacggcctg ctccaggacc tcggccacgt
     3181 gctcgaggcc tccggcgtcg gcgccaccgt ggcgctcgag cgcgtcccgc tcgcgccggg
     3241 cgccgctgct gcgggcgagg cagccggcct cgccatggcg ctcgacggcg gggacgatta
     3301 ccagctgtgt ttcgcggtcc cggccgcgcg cgagtcggag ctgctggccg cggccgccgg
     3361 ctgggatgcg ctgccggtgc ggatcggcgc cattcgcgcc gagccgggac tcgtgctcac
     3421 ccgggacggg cagccggtca cgcggccggc gggcggctgg gatccgtttc gcgaggtggc
     3481 gccatgagtg cgctatcgcc gcgcctgctg cgcgatccgg tgcatttgct cgcgctgggc
     3541 ttcggcaccg ggctggcgcc ccgggcgccg ggcacggccg gcacgctggt cggcgtgctg
     3601 ctggacccgc tgctgcggcc gctgggcctg gagctgcgga tcctggtggt ggcgctgatg
     3661 ttcggcgcgg gcgtgtggct gtgcggcgag agcgcccggc ggctgggcgt gcatgatcat
     3721 cccgccatcg tctgggacga gatcgtcggc tacctggcgc tgatgctcgt gcttccggcg
     3781 ggctggggct gggcattggc cggattcgtc gcgttccgct tcttcgacat cgtcaaaccg
     3841 tggccgattc gccaactgga tcacggagtc ggcggggggc tgggcattat ggtggacgac
     3901 gtcatggcgg cggcctgggg ggcagtggtg ctcctcggga tcgggttcgc cctggggatt
     3961 caatgagcgt cgagccgaaa gaaatggatg cctcgcaccc gtccaagggt gaaatgccgg
     4021 acaagatcgg caagtacagc atcatccgcg aagtgggccg gggcagctcg ggcacggtct
     4081 acctgtcgca cgaccccttt tacggccgcg acgtcgccat caagctgtac aaggaaccca
     4141 gcggcgagga cgagagcgag ctgcgggtcg cgcgcaagat gttcttcaac gaggcgcaga
     4201 tggtgggtcg gctgcagcac ccgaacatcc tgcccatcta cgacgccggc gaggagaacg
     4261 gtcgctatta cgtcgtcatg gagcacgtgc acggcgcgcg cacgctgtcg gcctattgcc
     4321 ggcccgacaa cctgctgccg atcgaggaag tcgtccggat cattttcaat accgtgcgcg
     4381 ccatgcacta cgcccacacc cgcggcgtcg tgcaccgcga catcaagccc agcaacatca
     4441 tgctcaccat gagcaacgaa gtgcgggtca tcgacttcgg catcgccctg tgggtggaca
     4501 acgaggtgtc cgtcatccgc ggcgtcgccg gctcgcccag ctacatgtcg ccggagcaga
     4561 tcaaggccga ggaaatcggc ccgcgctccg acctgtacgc actcggggcg gtgatgtacg
     4621 agctgctcac cggccggcgg cccttcaagg ccgccaacct cgccaagctg ctgcaccaga
     4681 tcgtgttcgc cacgccgcac ccggtgcaca agctgcggcc cgacgtcccg gaggaactcg
     4741 aggaagtggt gtggcgggcg atgcgcaaga agcccgaaga ccgcttcagc aacgggctgg
     4801 agttcgccgc tgcgctcacg cgcattcacc agaccttgcg cagcacccgc gatgcgctcg
     4861 acgagcgcga gcgggtcgcc atcctgcgcg ggctgcgttt cttccacgac ttctcgcata
     4921 ccgagatccg cgagctgata aaggcggccg aatggcgcga gtacgcgccg caacaggaga
     4981 tcgttcgcca gggcgagatg gacgatcgtt tctacgtcat catctccggc gaagtcgcag
     5041 tggaagtgga cggccagaag gtcggccacc tgatgccggg cgactgcttc ggcgaaagca
     5101 gcgtggtgcc caacgcccgc cgccaggcga ccatccgggc gctcggcgcc gtgacgctgc
     5161 tgcaggtcag ctcgaccctg ctggagacgc tctcggccgc ctgccagctg cgcttcaacc
     5221 gcatgttcct gcagacgctg atccagcggc tccagggtag cgggcacgcc aacgcctgac
     5281 cgacgactcc gcggcgcgtt cgaccgcgag ccggggctac ggtcgaccgc gagccggggc
     5341 cgcatgaggc ccccctcgct cggcgaaatc tttagctcgc tcggtgaacc tcatgcgtcc
     5401 ccggcccgct gcgctatcgc tggaggcgcg ctggccgcgc caccgacgca atcgctgaca
     5461 cttctggcag gcgcaatcgc tgtggtcggg gaggcgccga tccgtcgcag gcgtagcggc
     5521 cgcgggcgtg cggggcgggc tgcgcggcgc tcgcgcacag gacgtnnnnn nnnnncgagc
     5581 gagcctacag ggacgtattc acggcgtccg cgcaggtcgc ccggcacggc tgcggccgcg
     5641 gcaattctcg tgcatgatct gcccccggat gaggcacgaa acagggtagc gatatggcga
     5701 gcgaagtgca ggcaatgggc ctgtggcggc ggctgggcgg cagcgagctc ggacgctggc
     5761 tgttcacccg catcgtgtgc gtcaaggccc cgtacttcgc gtccatctcg ccgcgcctca
     5821 cgcggctgga gcccggccgc tgcgaggcgt acctgaagca ccgccggcgc gtgcagaacc
     5881 acatcggcac cgtgcacgcc atcgcgctgt gcaacctcgc cgagctgtgc gccggcctgg
     5941 tcaccgacgt cagcatcccg cgcgacatgc gctggattcc gcaggacatg accgtgcgct
     6001 acctgaagaa agccgccggc aggatctccg ccatcgccac gccggcgacc gaacttcgct
     6061 caaccgccga gccctatccc gccaccatcg aggtcaggct gagcgacgcc gccggccagc
     6121 tggtcgccag cgccgacatc cacatgtggc tcagcccgcg acccggccag caagcccgaa
     6181 aagcctgagc gatcgacgag ccggcagccg atcggtcgac atcgcagctg ccgagccgcc
     6241 gacaatgtac cggccagtcg acgttgcagc ggctgggtgt gtcggtggcg gcctgcgcgg
     6301 cgctcgcgca cagga
//
LOCUS       DAOMPG010000137         6293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5283_length_6293_cov_4.138346, whole genome shotgun sequence.
ACCESSION   DAOMPG010000137 DAOMPG010000000
VERSION     DAOMPG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_6293_cov_4.138346"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLS34_08270"
     CDS             <1..276
                     /locus_tag="PLS34_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRP87500.1"
                     /translation="ADVEWESNHRQVETVQAGVADGTLARAVESRIRLDDELAREDIT
                     VSSTGDVVTLHGRLSKVALLERALRIASEVQGVSRVVSRLTVEMEAG"
     gene            278..1369
                     /locus_tag="PLS34_08275"
     CDS             278..1369
                     /locus_tag="PLS34_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRP87501.1"
                     /translation="MRALTTLAFTALLALAAVPPAGAVERTLELRIDGSRPVTVENLV
                     GAVRVVPGDKDFVIRATVSADQQSVADAVQLRREDGRSSVAVVVEYPENVSRLRYDGA
                     EIRRIDASIDYQGRRLRITSSSGERVSVDLEIAVPADARLGLKQAVGPVTADRVAADL
                     SLAARFGRIVVTDGAGRLRADTGSGTISVASFRGSVVADTGSGGVTIENVLGKVVADT
                     GSGSVRLRGVEGDVVADTGSGSVRLDDVIGSLHVDTGSGSVRGENIVAGPELLVDTGS
                     GSVSLSGDLGAVRRLVVDTGSGSVALQSSSPLSLRLELATGSGGIRVDVPAISNVDSS
                     RRRFRGVIGAGEGTAKVSTGSGSVRISAP"
     gene            complement(1424..2605)
                     /gene="purT"
                     /locus_tag="PLS34_08280"
     CDS             complement(1424..2605)
                     /gene="purT"
                     /locus_tag="PLS34_08280"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRP87502.1"
                     /translation="MTTLGTPLTDNATRLLLLGAGELGKEVAIEAQRLGVEVIAVDRY
                     PNAPAMQVAHRSHVIDMLDRKALRALIEAERPSLVVPEIEAIATQELLALEAEGYRII
                     PTAAAARLTMDREGIRRLAAEELGLATSPFRFADTVEEYRSAIAAIGLPCVVKPVMSS
                     SGKGQSTVRSEADIEPAWQYSQQGGRTGAGRIIVEGFVEFDYEITLLTVRHGGTTSFC
                     APIGHLQVDGDYRESWQPQPMSALALERARAIADAVTRRLGGFGIFGVELFVRGDEVL
                     FSEVSPRPHDTGLVTLASQSLSQFALHVRAILGLPVPDIRQWGPAASCAILGAGDSDR
                     IRYAGVDEVLQEPDTELRLFGKPEVHGKRRLGVVLARGGSIEEARDKARRAAGRLRIE
                     L"
     gene            2709..3836
                     /locus_tag="PLS34_08285"
     CDS             2709..3836
                     /locus_tag="PLS34_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRP87503.1"
                     /translation="MTDPVRPSLGPVGALLGAAAVVIIIGGLKLAAPLVVPFLLAAVL
                     ATILIPPLHWLTDRRVPLPLAMLILSLGLVLVWIPLAAVVGASFDDFYKELPAYQEKI
                     QAMVASVAAWLGEHGVEAGRAAIEDLVKPAAALSFVRQIGGGLGTALANIFLILLTII
                     FILFEASGFPAKVRAALGDRGDVLGSFREFSERLQEYLRIKTLVSLMTGGTIALWLWI
                     VGLDFAMLWGVLAFLLNYVPNIGSIIAAVPAVLLALVALDWTGVLLVVAGYAAVNMVF
                     GNIIEPKMMGKGLGLSTLVVFLSLVFWGWVLGPVGMLLSGPLTMAVKIALETDPRSRW
                     FAVLLGPGGQPPPAAAQDAEAAANDAASPAGGGSGTVQGDA"
     gene            3833..4498
                     /gene="can"
                     /locus_tag="PLS34_08290"
     CDS             3833..4498
                     /gene="can"
                     /locus_tag="PLS34_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HRP87504.1"
                     /translation="MTDRRSEDIRQLFQSNRTWAARIRERDPEFFLKLSAQQSPRYLW
                     IGCADSRVPANEIVDLMPGELFVHRNVANLVVHTDLNCLSVIQFAVDVLKVEHVIVCG
                     HYGCSGVGAAFRGDRLGLSDNWLRHVQDVHARHRDCISADGGEDAVNRLCELNVVEQV
                     VNVCSTTVVQEAWERGQSLAVHGWVYGLRDGLIKDLEVSVSRPGDLAPAYGAAVSRLR
                     AMP"
     gene            complement(5081..5737)
                     /locus_tag="PLS34_08295"
     CDS             complement(5081..5737)
                     /locus_tag="PLS34_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP87505.1"
                     /translation="MGESARIIVADDHPLFREALQQALAPMMPGVSFIEAESFESLQA
                     AVGECEDADLVLLDLEMPGAQGFSVLAWLRTQHPALPVVVVSATSDAGVMRRAVDFGA
                     SGFIPKSSPVETISEAIAAVLEGEIWLPESALALDDSSLSEHEDLAKRVASLTPQQFR
                     VLDMLAEGLLNKQIAAELQVSEATVKAHVTAIFRKLGVRSRTQAAVAARRLEASARPD
                     "
     gene            complement(5834..>6293)
                     /locus_tag="PLS34_08300"
     CDS             complement(5834..>6293)
                     /locus_tag="PLS34_08300"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493644.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HRP87506.1"
                     /translation="TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESALVAHPK
                     VAEAAVVGFPHDIKGQGVYCYVTLQAGEEPSDALGKELRAWVREEIGPIATPDVIQFA
                     PGLPKTRSGKIMRRILRKVAAHEYDSLGDTSTLADPAVVTDLIDNRKKLD"
BASE COUNT          885 a         2172 c         2265 g          971 t
ORIGIN      
        1 gccgacgtcg agtgggaatc gaatcaccgc caggtcgaga cggtgcaggc gggcgtggcc
       61 gacggcaccc tggcgcgcgc agtcgagtcg agaatccgcc ttgacgatga gctggcccgc
      121 gaggacatca cggtgtcgtc gaccggcgac gtcgtcacgc tgcatggccg gctgagcaag
      181 gtcgcgctgc tggagcgggc gttgcgtatc gcgagcgaag tgcagggcgt gtcgcgggtc
      241 gtgtcgcgac tcaccgtaga gatggaggca ggctgacatg agagcactga cgaccctggc
      301 attcaccgcc ctgctggcgc tggccgcggt gccgcccgcg ggcgcggtgg agcggacgct
      361 cgaactgcgc atcgacggct cccggcccgt caccgtggag aacctggtcg gtgcggtccg
      421 cgtcgtgccg ggagacaagg acttcgtcat ccgcgccacg gtgtccgccg accagcagtc
      481 ggtcgccgac gccgtccagc tgcgccgcga ggacggccgc agcagcgtcg cggtcgtggt
      541 ggagtacccg gaaaatgttt cgcgcctccg ttacgacggt gccgagatcc gtcgcatcga
      601 cgcgtccatc gactaccagg gacgcaggct gcgcatcacg agttccagcg gcgagcgggt
      661 cagcgtggac ctggagatcg cggtgcctgc cgacgcccgg ctcggcctga agcaggcggt
      721 ggggccggtc actgcagatc gcgtcgccgc agacctgtcg ctcgccgccc ggttcggccg
      781 catcgtggtc accgacggcg cggggcggct gcgcgccgac accgggtcgg ggaccatcag
      841 cgtggccagc ttccggggca gcgtggtggc cgataccggc agcggcgggg tcaccatcga
      901 gaacgtgctg ggcaaggtcg tagcggacac cggttccggc agcgtccggc tgcgcggcgt
      961 cgagggcgac gtggtggcgg acacgggctc cggcagcgtg cggctggacg acgtcatcgg
     1021 ctcgctgcac gtcgataccg gctcgggctc ggtgcgcggc gaaaacatcg tggccggccc
     1081 ggagctgctg gtggacaccg gctccggcag tgtgtcgttg tcgggcgatc taggtgcggt
     1141 gcgccggctg gtggtcgaca ccggctcggg cagcgtggcc ttgcagagca gcagcccgct
     1201 ttccctgcgg ctggagctcg cgaccggcag cggcggcatc cgcgtggatg tgccggcgat
     1261 cagcaacgtc gactcgagcc gccgcaggtt ccgcggcgtg atcggcgccg gcgagggcac
     1321 cgcgaaagta tccaccggca gcggcagcgt gcgcatcagc gcgccctagc cggccggcac
     1381 aaactccccc tcggggtgcc gcccgtgtgc ggcgcccctt tttttacagc tcgatgcgca
     1441 accgcccggc ggcgcgacgt gccttgtcgc gcgcctcctc gatgctgccg ccgcgcgcca
     1501 gcacgacgcc gaggcggcgc ttgccgtgga cctccggctt gccgaacagg cgcagctcgg
     1561 tgtccggttc ctgcagcacc tcgtccacgc cggcatagcg gatccggtca gagtcgcccg
     1621 cgccgagaat cgcgcacgag gctgccgggc cccactggcg gatgtcgggc acgggcagcc
     1681 cgagaatggc gcgcacgtgc agcgcgaact gcgacaggct ctgcgaggcg agcgtcacca
     1741 gcccggtgtc gtgaggacgc ggcgacacct cgctgaacag cacctcgtcg ccgcgcacga
     1801 acagctccac gccgaaaatg ccgaagccgc cgagccgccg ggtgacggcg tcggcgattg
     1861 cgcgggcgcg ctccagcgcc agtgcgctca tcggctgggg ctgccaggat tcgcggtagt
     1921 cgccgtccac ctgcaggtgg ccgatgggcg cgcagaagct ggtcgtgccg ccgtggcgca
     1981 cggtcagcag ggtgatttcg tagtcgaact cgacgaagcc ttcgacgatg atccggccgg
     2041 cgcccgtgcg cccaccctgt tgcgagtact gccaggccgg ctcgatgtcc gcctcgctgc
     2101 gcaccgtcga ctgccccttg ccggaggagc tcattaccgg cttgacgacg cagggcagcc
     2161 cgatggccgc gatcgcgctt cgatactcct ccaccgtgtc ggcgaagcgg aacggcgagg
     2221 tcgccaggcc gagttcctcg gcagcgagcc tgcgaatgcc ctcccggtcc atggtcaatc
     2281 gcgcggccgc ggcggtcgga atgatccggt agccctcggc ctcgagcgcc agcagttcct
     2341 gcgtggcgat ggcctcgatc tccggcacca ccaggctcgg tcgctcggcc tcgatcaggg
     2401 cgcgcagcgc cttgcgatcg agcatgtcga tgacgtggct gcggtgcgcc acctgcatcg
     2461 caggggcatt cgggtagcgg tccacggcaa tgacctcgac gccgaggcgc tgcgcttcga
     2521 tggcgacttc cttgcccagc tcccccgcgc cgagcaacag caggcgggtg gcgttatcgg
     2581 tcagcggcgt gcccagcgtc gtcatggctc tctcgcggca gttgatcccg ccggcgatac
     2641 ccgccggccc gccgcgtatc gtactctatg ggcccgcagc aggttcgcag caaagtggcg
     2701 cagcacgcat gacagaccca gttcgcccgt ccctgggacc cgtcggggcc ctgctcgggg
     2761 ccgccgccgt ggtgatcatc atcggcggcc tgaagctcgc cgccccgctg gtggtgccct
     2821 tcctgctggc agcggtgctg gcgacgatcc tgatcccgcc gttgcactgg ctcaccgacc
     2881 ggcgggtgcc gctgccgctg gccatgctga tcctgtcgct gggcctggtg ctcgtctgga
     2941 tcccgctggc tgcggtggtg ggcgcttcct tcgacgattt ctacaaggag ctgccggcct
     3001 accaggagaa gatccaggcg atggtggcct cggtcgccgc ctggctgggc gaacacggcg
     3061 tcgaggcggg gcgagcggcg atcgaggacc tggtcaagcc ggcggcggcg ctgtccttcg
     3121 tccggcagat cggcggcggg ctcggtacgg cgctggcgaa catcttcctg atcctgctga
     3181 ccatcatctt catcctgttc gaggcctccg gctttccggc caaggtgcgc gcggcgctcg
     3241 gcgaccgggg ggacgtgctg ggctcgtttc gcgaattttc cgagcggctg caggagtacc
     3301 tgcgcatcaa gacgctggtc agcctgatga ccggcggcac gatcgcgctg tggctgtgga
     3361 tcgtggggct cgacttcgcg atgctgtggg gcgtgctggc cttcctgctg aactacgtgc
     3421 cgaacatcgg cagcatcatc gcggccgtgc ccgccgtgct gctcgcgctg gtggcgctcg
     3481 actggaccgg cgtgctgctg gtggtcgcgg gctacgccgc ggtcaacatg gtgttcggca
     3541 acatcatcga gccgaagatg atgggcaagg gtctcggcct ctcgacgctg gtggtgttcc
     3601 tgtcactggt gttctggggc tgggtgctgg ggccggtggg catgctgctg tccggcccgc
     3661 tgaccatggc cgtcaagatc gccctcgaga ccgacccgcg ctcccgctgg ttcgcggtgc
     3721 tgctggggcc gggcggtcag cccccgccgg cggccgcgca ggatgccgag gcggcggcga
     3781 acgacgccgc gtcgccggcg ggcggcgggt cgggcactgt acagggcgac gcatgaccga
     3841 ccggcgcagc gaggacatcc gccagctgtt ccagagcaat cgcacctggg cggcgcgcat
     3901 tcgtgagcgc gacccggagt tctttctcaa gctttcggcc cagcaatcgc cgcgctacct
     3961 gtggatcggc tgcgccgaca gccgcgtgcc ggccaacgag atcgtcgact tgatgcccgg
     4021 cgagctgttc gtccaccgca acgtggccaa cctggtggtc cataccgacc tgaactgcct
     4081 gtcggtcatc cagttcgccg tcgacgtgct caaagtcgag cacgtcatcg tgtgcggcca
     4141 ctatggctgc agcggcgtgg gcgcggcctt ccgtggcgac cggcttggcc tgagcgacaa
     4201 ctggctgcgc cacgtgcagg acgtgcacgc ccgccaccgg gattgcatct cggcggacgg
     4261 cggcgaggat gcggtcaacc ggctgtgcga gctcaacgtc gtggagcagg tggtcaacgt
     4321 ctgcagcacg accgtggtcc aggaagcctg ggagcgcggc cagtcgctcg cggtgcacgg
     4381 ctgggtgtac gggctgcgcg atggcctgat caaggacctc gaagtcagcg tcagccggcc
     4441 gggagatctc gcgcccgctt acggcgcggc cgtcagccgg ctgcgcgcca tgccctgacg
     4501 gaagcccggg aaaaccgggg tcagacaccg gtttccagtc gaacgccggc gccttcggtt
     4561 ccagtcgacc gcgggcgcgc ggggccttcg gttgcggtcg accgcgggcg cgcggcgcca
     4621 taggccggcc tgcgctcgga caaggaaaaa ctcgctcggc cggcctatga caccccacgc
     4681 ccgctgccgg gatcacaagg ctggcggcga caatggttgc cgagccgagc cgagccgccc
     4741 ggggtcgccg atgcgcggcg gcagcgtcca gtgccgaacg cctgcgacga cgacgggtca
     4801 gtccaccccg gcaatgccga tgcccgcgtg gcaatggtca gcggcgccac aaaggcaagg
     4861 cgaccccccc gtctgcgcgg cagcgggacc ggggtgtcat gggcgcgctg agtgagctca
     4921 gacttcgccg aacgaagcgc gcccatggcg gcccggtccc gcggacaacc gccaaccgaa
     4981 gcgcgcccat ggcggcgcgg tcccgcggac aaacggaacc aatggcgccc atggcggcgc
     5041 atctccgcgg tcaacccgaa accggtgtct gaccccggtt tcagtcgggg cgagcggagg
     5101 cctcgaggcg gcgcgccgcg acggcggcct gggtgcggct gcgcacgccg agcttgcgga
     5161 agatcgccgt gacgtgcgcc ttgacggtcg cctcggacac ctgcagctcg gccgcgatct
     5221 gcttgttgag caggccctcg gcgagcatgt ccagcacacg gaactgctgc ggcgtcaggc
     5281 tggcgacgcg cttggcgaga tcctcgtgct cggacaggct cgagtcgtcc agcgccagcg
     5341 cggattcggg cagccagatc tcgccctcca ggaccgcggc gatggcctcc gagatcgtct
     5401 ctaccggcga agacttgggg atgaagccgg aggcgccgaa atcgaccgcg cggcgcatga
     5461 cgccggcgtc gctggtggcg gacaccacca ccaccggcaa cgccgggtgc tgcgtacgca
     5521 accaggcgag cacggaaaag ccctgcgcgc cgggcatctc gagatccagc agcacgagat
     5581 ccgcgtcctc gcattcgccc actgcagcct gcagggactc gaaggactcc gcctcgatga
     5641 agctcacgcc gggcatcatc ggcgccagcg cctgctgcaa agcctcccga aagagggggt
     5701 ggtcgtcggc gacgatgatg cgcgcgcttt cgcccatgag tccgtcatcc cggttcgccg
     5761 cgccgggcgg cgggccccgg cgcggtgatt caattgcctg gcatccgtgc cgccgctcca
     5821 gtagcggcag gcgtcagtcc agcttcttgc ggttgtcgat caggtccgtg accacggccg
     5881 gatcggccag ggtcgaggtg tcgccgaggc tgtcgtactc gtgcgccgcg accttcctca
     5941 agatgcgccg catgatcttg ccggagcggg tcttgggcag tcccggcgca aactggataa
     6001 cgtcgggcgt ggcgatcggg ccgatctcct cgcgcaccca ggcgcgcagt tccttgccga
     6061 gcgcgtcgct cggctcctcg cccgcctgca gggtgacgta gcagtacacg ccctggccct
     6121 tgatgtcgtg cgggaagccc accaccgcgg cttccgccac cttcgggtgg gccaccagcg
     6181 cgctctcgat ctcggcggtg cccatgcggt gcccggagac gttcagcacg tcgtccacgc
     6241 ggccggtgat ccagtagtag ccgtcctcgt cgcgccgcgc gccgtcgccg gtg
//
LOCUS       DAOMPG010000138         6269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5311_length_6269_cov_4.958159, whole genome shotgun sequence.
ACCESSION   DAOMPG010000138 DAOMPG010000000
VERSION     DAOMPG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5311_length_6269_cov_4.958159"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..983
                     /gene="hflC"
                     /locus_tag="PLS34_08305"
     CDS             105..983
                     /gene="hflC"
                     /locus_tag="PLS34_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRP87507.1"
                     /translation="MSSRATFLLLIVAAAAFLLSMSLFVVDEREHAIKLRFGEIVRVG
                     YEPGLHFKTPFVNTVRKFDNRVLTRNNPNEPFLTAEKKNLRVDFYVKWRITDVGQYFR
                     ATAGDERLATSRLLEIIKDGLRAQFARQTLQEVVTADRREVIEDLMGSAGVTARELGI
                     EIVDVRVKTLDLPPDVSDSVFNRMRQERARVASQLRAEGAETAERIRSDADRQRTVII
                     AEAQRDSQRIRGVGDARAADIYAEAFGRDRGFYDFWRSMQAYRTSIGRGDDVLVIEPD
                     GEFFRYLKDPTADQAR"
     gene            1000..1191
                     /locus_tag="PLS34_08310"
     CDS             1000..1191
                     /locus_tag="PLS34_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRP87508.1"
                     /translation="MNWTDLLSAIALLMVIEGLLPFANPRGSRRTMAMLAQMPDEKLR
                     LAGLVSIIAGLVLLWVVRS"
     gene            1217..2509
                     /locus_tag="PLS34_08315"
     CDS             1217..2509
                     /locus_tag="PLS34_08315"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRP87509.1"
                     /translation="MGKNVVVIGTQWGDEGKGKVVDLLTDRVAAVVRFQGGHNAGHTL
                     VIGGKKTIVRLLPSGILREGVSCLIGNGVVLSPEALLGEIDELTGRGIPVIERVRISP
                     ACPLIMPSHVALDAAREHAKGAAKIGTTGRGIGPAYEDKVARRALRVSDLAHRERFAA
                     KLGEVLDFHNFVLQRYFGREAVDFQQTLDHLLALGERIQPMVADVVALLDGHRKAGDN
                     VLFEGAQGALLDIDHGTYPFVTSSNTTAGGACTGAGVGPRSIDYVLGIVKAYTTRVGS
                     GPFPTELFDEMGEHLARVGHEFGSVTGRARRCGWFDAVSLRRSISLNSVSGLCVTKLD
                     VLDALDTIRICVGYRVGGKVTETPPLAADQYADIEPIYEDVPGWNSSTVGITRHADLP
                     ANARAYLERLAEVVGVPIDIISTGPDRAETIVLRNPFD"
     gene            complement(2556..3017)
                     /locus_tag="PLS34_08320"
     CDS             complement(2556..3017)
                     /locus_tag="PLS34_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRP87510.1"
                     /translation="MNKLWIVVIVVALLVLAVGGVAGHQMTKGDAFCSSCHAYERASW
                     DHGMHPDVGCLECHTGGFMRDKTQGARKVFLVFTGQVNPHHDKLASYPDKTMNNCIGC
                     HLSDEAVQANPIFATRHSEYRQAAEYCIACHEAGHVERIRNMRYLSVRRTE"
     gene            complement(3081..4625)
                     /locus_tag="PLS34_08325"
     CDS             complement(3081..4625)
                     /locus_tag="PLS34_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRP87511.1"
                     /translation="MRYLIALVTGAVLVGIGLSLSLRSADSASAAPEGAYGAAPSEWK
                     YGEGNLKFQNLPQYKSYLANNDDSQMTEYAGSVQHQKHNDFDPLPRGYKHAQPYLKNL
                     WLGYPFSYEYDRARGHTYAITDVTKIDRINRYSEQAGLPTTCFNCKTNAMPQLLKEYG
                     DALWAMNFHDFREQLDNERHSIGCENCHDPANHMRLVITSVPLDEALKRQGKDWREAS
                     RHEMRSLVCAQCHVEYYFEKGEHGVAAKPHFPWDYGMNAEDIYRFKADGQAERDGFTG
                     PFADWVHAVSKTPMVKVQHPEYEMFSDSVHAQNGVSCADCHMPRVTNGPVRITSHHWT
                     SPLKNDESIKSSCGTCHSDRSPADLRERTEYNQERVWKQLNIAQEKSVRAHEAVRRAM
                     ETPGVDQEVLAQARELVRKGQWFWDYVSAENSAGAHNPVKALDTLASSQQYSDEAVQL
                     AIRASNYAIAAAMDRPINELVPPIREHSRKLQQSQAHLDSHPWLQYLPLLPEAELVWD
                     GNKRVK"
     gene            complement(4780..6150)
                     /locus_tag="PLS34_08330"
     CDS             complement(4780..6150)
                     /locus_tag="PLS34_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP87512.1"
                     /translation="MNQTIRYARDNDGQNVAYEVSGDGPLDLVFIPDWVTNLEVMREE
                     PTIARFYDRLASFSRLICLDKRGSGLSDPVPLGAIPTIEEWMHDVCTVLDDLGCGRAA
                     LFAHGEGGPLALLFAATHPDRTHAIVLADTCARRRRGPDYPAGLPDDAAQRYIDDIIG
                     AWGSGEAAYIGAPSEAAHPGFVERRGRLERLAIIPGEFAAIYPSTYDLDIRPVLPTVR
                     VPTLVLHRRDNHYMRVGNGRYLAEHIDGATLVELPGRDHFFHAGDVVEMLDHVQQFLT
                     GTREAPDHDRVLATVLFTDIVGATGLAERLGDRDWKNLLDRHHVLVRQELARFRGREI
                     DTAGDGFFATFDGPARGVRCALAIRDVVQTLGIEVRAGLHIGECEVMGPKVGGIAVHI
                     AARIMGLCRPGAVLVSRTVKDLVAGSGLQFAPAGRHALRGVGEEWDLYVAMGRPPEPA
                     GSAWPP"
BASE COUNT          968 a         2060 c         2161 g         1080 t
ORIGIN      
        1 cagacgcggc tcggtgaccc ggccgatctc ggccaggcca cgccgctgcg ccagacggag
       61 cgccagggtt cgcccgacga cctgcgcgcc agggggaccc gctgatgagc tcgcgagcca
      121 cattcctgtt gctgatcgtc gccgcggcgg cctttctgtt gtcgatgtcg ctgttcgttg
      181 tcgatgagcg cgagcacgcc atcaagctgc gcttcggcga gatcgtgcgt gtcggctacg
      241 agcccggcct gcatttcaag acgccgttcg tcaacacggt gcggaagttc gacaaccgcg
      301 tgttgacccg taacaacccg aacgagccct tcctgaccgc ggaaaagaag aacttgcggg
      361 tggacttcta cgtgaagtgg cgcatcaccg atgtcggcca gtacttccgt gccacggccg
      421 gcgacgagcg cctcgccacc agccgcctgc tggaaatcat caaggacggg ctgcgcgccc
      481 agttcgcgcg ccagaccctg caggaggtcg tgaccgcgga ccggcgcgaa gtcatcgagg
      541 acctgatggg ttcggcgggc gtcactgcgc gcgagctcgg catcgagatc gtcgatgtcc
      601 gcgtcaagac gctggacctg ccgccggacg tcagcgacag cgtgttcaac aggatgcgcc
      661 aggagcgcgc ccgggtcgcc tcgcagctgc gcgcggaagg cgcggagacg gccgagcgca
      721 tccgctcgga cgccgaccgg cagcgcaccg tcatcattgc cgaggcgcag cgtgattcac
      781 agcgcatccg cggcgtcggc gacgcgcgcg ccgcggacat ctacgcggag gccttcgggc
      841 gcgatcgcgg cttttatgac ttctggcgca gcatgcaggc ctaccggaca tccatcggcc
      901 ggggcgacga cgtgctggtg atcgagcccg acggcgaatt cttccgttac ctgaaggacc
      961 cgacggccga ccaggcccgc tgaacggcgc caggcggcca tgaactggac cgacctgctg
     1021 tcggcgatcg ccctcttgat ggtgatcgag ggcctgctgc cgttcgccaa cccgcgcggg
     1081 tcgcgccgca ccatggccat gctggcgcag atgccggacg aaaagctgcg gctcgcggga
     1141 ctggtgagca tcatcgccgg cctggtgctg ttgtgggtcg tgcgttcctg aacggctgag
     1201 gcaaaggagc tcgagcgtgg ggaaaaacgt ggtcgtcatc ggcacccagt ggggtgacga
     1261 gggcaagggc aaggtcgtgg acctcctgac cgaccgcgtg gcggcggtgg tgcgcttcca
     1321 gggcgggcac aacgcgggcc atacgctggt tatcgggggc aagaaaacca tcgtgcggct
     1381 gctgccctcg ggcatcctgc gcgagggcgt gtcctgcctc atcggcaacg gcgtggtgct
     1441 ctcgcccgag gccttgctgg gcgagatcga cgagctgacc gggcgcggca ttcccgtcat
     1501 cgagcgtgtg cgcatcagtc ctgcctgccc gttgatcatg ccctcgcacg tcgcgctgga
     1561 tgcggcgcgc gagcacgcca agggcgccgc caagatcggc accaccgggc gcggcatcgg
     1621 cccggcctac gaggacaagg tcgcacgccg cgccttgcgg gtctcggacc tggcgcatcg
     1681 cgagcgattt gccgcgaagc tgggcgaggt gctcgacttc cacaatttcg tgctgcagcg
     1741 ctatttcggc cgcgaggcgg tggatttcca acagacgctg gatcacctgc tggcactcgg
     1801 cgaacgcatc cagccgatgg tcgccgacgt ggtggcgctg ctcgacggcc accgcaaggc
     1861 aggcgacaac gtgctgttcg agggcgcgca gggcgccttg ctggacatcg accacggcac
     1921 ctatcccttc gtgacctcgt ccaataccac ggccggcggc gcgtgtaccg gcgccggcgt
     1981 cgggccgcgc tcgatcgact acgtgctcgg gatcgtgaag gcctacacga cgcgcgtcgg
     2041 ctccggcccg ttcccgaccg agttgttcga cgagatgggc gagcacctgg cgcgcgtggg
     2101 ccacgaattc ggctcggtga cggggcgggc gcgccgctgc gggtggttcg acgccgtgtc
     2161 gctgcgccgc tccatctcgc tgaacagcgt ctccgggcta tgcgtcacca agctcgacgt
     2221 gctcgatgcg ctcgacacca tccgtatctg cgtcggctac cgggtcggcg gcaaggtcac
     2281 ggagacgccg ccgctggccg ccgaccagta cgccgatatc gagccgatct acgaggacgt
     2341 gccgggctgg aacagctcga ccgtcggcat cacgcgccac gcggacctgc ccgccaacgc
     2401 gcgtgcctat ctcgagcggc tggcagaggt cgtcggcgtg ccgatcgaca tcatctcgac
     2461 cgggcccgac cgtgccgaga cgatcgtgct gcgcaatccc ttcgattaat ggggacactc
     2521 cccaaatccg agggaattgg ggagtgtccc caatttcact ccgtgcgccg cacgctcagg
     2581 tagcgcatgt tgcggatgcg ttccacgtgg ccggcctcgt ggcaggcgat gcagtattcc
     2641 gcggcctgcc ggtactccga gtggcgcgtg gcgaagatcg ggtttgcctg caccgcctcg
     2701 tcggagaggt ggcagccgat gcagttgttc atggtcttgt cgggatagct cgccagcttg
     2761 tcgtgatgcg ggttcacctg cccggtgaac accaggaaca ccttgcgcgc gccctgcgtc
     2821 ttgtcgcgca tgaagccgcc ggtatggcat tcgaggcagc cgacgtcagg gtgcatgccg
     2881 tgatcccacg acgcgcgctc gtacgcatgg cacgagctgc agaatgcgtc gcccttggtc
     2941 atctggtgac ctgcgacgcc gccgacggcc aacacgagca gcgccacgac gatcaccacg
     3001 atccagagtt tgttcatgtc ttcgactctc caggttccgc ctggtgaagg accggcgggc
     3061 gcggggcccg ccggcgatcg tcacttgact cgcttgttgc cgtcccagac cagctcggcc
     3121 tccggcagca gcggcaggta ttgcagccac gggtgggagt ccagatgcgc ctggctctgc
     3181 tggagcttgc ggctgtgctc gcgaatcggc ggcaccagtt cattgatcgg ccggtccatg
     3241 gcggccgcaa tcgcgtaatt ggaggcgcgg atcgcaagct ggaccgcctc gtcgctgtac
     3301 tgctgcgagg aggccagggt gtcgagcgcc ttgaccgggt tgtgggcgcc ggcgctgttc
     3361 tccgccgaca cgtagtccca gaaccactgg cccttgcgca ccagctcgcg tgcctgcgcg
     3421 aggacttcct ggtcgacgcc cggcgtctcc atcgcgcggc gcaccgcctc gtgggcgcgc
     3481 accgatttct cctgcgcgat gttgagttgc ttccagacgc gctcctggtt gtactcggtg
     3541 cgctcgcgca ggtcggccgg cgagcggtcg gagtgacagg tgccgcagga ggacttgatg
     3601 gattcgtcat tcttcagcgg cgaggtccag tggtgcgagg tgatgcgcac cgggccgttg
     3661 gtgacgcgcg gcatgtggca atcggcgcag ctgacgccgt tctgcgcatg cacgctgtca
     3721 gagaacatct cgtactcggg gtgctgtacc ttgaccatcg gcgtcttgga taccgcgtgc
     3781 acccagtccg cgaacgggcc ggtgaagccg tcgcgctcgg cctggccgtc cgccttgaag
     3841 cggtagatgt cctcggcgtt catgccgtag tcccagggga agtgcggctt ggcggccacg
     3901 ccgtgctcgc ctttctcgaa gtaatactca acatgacatt gcgcgcacac cagcgagcgc
     3961 atctcgtggc gcgacgcttc gcgccagtcc ttgccctggc gcttgagcgc ctcgtccagc
     4021 ggcacgctgg tgatgaccag gcgcatgtgg ttggccgggt cgtggcagtt ctcgcagccg
     4081 atgctgtggc gttcgttgtc gagctgctcg cggaagtcgt ggaaattcat cgcccagagc
     4141 gcatcgccgt attccttcag gagctgcggc atggcgttgg tcttgcagtt gaagcaggtg
     4201 gtcggcagtc cggcctgctc gctgtaacgg ttgatccggt cgatcttggt gacgtccgtg
     4261 atcgcataag tgtggccgcg ggcgcggtcg tactcgtagc tgaacgggta gccgagccac
     4321 aggttcttca ggtacggctg cgcatgcttg tagccacgcg gcagcgggtc gaagtcattg
     4381 tgcttctggt gctgcaccga acccgcgtac tcggtcattt gcgagtcgtc attgttcgcg
     4441 aggtacgact tgtactgcgg caggttctgg aacttcaggt tgccttcacc gtatttccac
     4501 tcggacgggg cggcaccgta ggctccctcc ggcgcagccg aggccgagtc cgcggaccgg
     4561 aggctgagcg acaggccaat accgacgagc accgcgcccg ttaccagtgc gatcagataa
     4621 cgcatgactg aactcctgct gtttccccgg ctcgccgcgt ggccgccgca agccctgtga
     4681 cacgactgcg tcgcgtcatc cctcccggta gtagagagat tccttcaaac cgtttgtata
     4741 gtggttaacc cctacatttc cggatggatc cccatgggct cagggtggcc acgccgaccc
     4801 cgccggctcc ggcgggcggc ccatggcgac gtacagatcc cattcctcgc cgaccccgcg
     4861 caaggcgtgt cgcccggcgg gcgcgaactg caggcccgag cccgccacga gatccttgac
     4921 cgtgcgcgac accagcaccg cgcccggccg gcagagcccc atgatacggg cggcgatatg
     4981 gacggcgata ccgccgacct tgggacccat gacttcgcac tcgccgatgt gcaggccggc
     5041 gcgaacctcg atgccgagcg tctggacgac gtcccggatg gccagcgcgc agcgcacgcc
     5101 gcgggccggg ccgtcgaagg tcgcgaaaaa gccgtctccg gcagtgtcga tctcacggcc
     5161 gcgaaaccgg gcaagctcct gccgcacgag gacatggtgg cggtccagca ggttcttcca
     5221 gtcccggtcg ccgagccgct ctgcgaggcc ggttgcgccg acgatgtcgg tgaacagcac
     5281 ggtcgcgagc acgcggtcgt gatccggcgc ttcacgggtg ccggtcagga actgctggac
     5341 gtggtcgagc atttccacca cgtcgccggc atggaaaaaa tggtcccggc cgggcagttc
     5401 gaccagcgtt gcgccatcga tgtgctcggc gaggtagcgg ccgttgccga cgcgcatgta
     5461 gtggttgtcg cgccggtgca gcaccagcgt gggtacgcgg acggtcggca gcaccgggcg
     5521 tatgtccagg tcataggtcg acgggtagat cgcggcaaac tcgccgggga tgatggccag
     5581 ccgctcgagc cgcccgcgac gttccacgaa gccgggatgg gcagcctcgc tgggcgcgcc
     5641 gatgtacgcc gcctcgccgg agccccaggc gccgatgatg tcgtcgatgt agcgttgcgc
     5701 cgcgtcgtcg ggcagcccgg cagggtagtc cggccccctg cggcgacgcg cgcaggtgtc
     5761 ggccagcacg atggcgtgcg tgcggtcggg atgggtcgcg gcgaacagca gggccagggg
     5821 tccgccttcc ccgtgggcga acagcgcggc gcgcccgcaa ccgaggtcat cgagcacggt
     5881 gcatacgtcg tgcatccatt cctcgatggt cgggatggcc ccgagcggca ccggatcgga
     5941 aaggccgctg ccgcgcttgt ccaggcagat caggcggctg aaggaggcga gccggtcgta
     6001 gaaccgcgcg atcgtcggct cttcgcgcat gacctccagg ttggtgaccc agtccgggat
     6061 gaacacgaga tccagcggcc cgtcgccgct gacttcatag gcgacgttct ggccgtcgtt
     6121 gtcgcgcgca tagcggatcg tctggttcat gctggctggg tcccgcgaag agaaaccctg
     6181 gcgcgcaccg catagcgcgc ggcgtcgccg gagggacttg tcggaccgag attacgagat
     6241 gccgggcgct ttcaccatct cgggcgagc
//
LOCUS       DAOMPG010000139         6261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5319_length_6261_cov_4.283597, whole genome shotgun sequence.
ACCESSION   DAOMPG010000139 DAOMPG010000000
VERSION     DAOMPG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5319_length_6261_cov_4.283597"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLS34_08335"
     CDS             <1..286
                     /locus_tag="PLS34_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRP87513.1"
                     /translation="LFATHAHWIAGRPPRLPLACTARARHRQPEQPCRVEQGGAEGTL
                     QVRFEQPQRALTPGQFIVFYAGEECLGGATIASVGAPFMPAAGAPRAASA"
     gene            397..3153
                     /gene="acnA"
                     /locus_tag="PLS34_08340"
     CDS             397..3153
                     /gene="acnA"
                     /locus_tag="PLS34_08340"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRP87514.1"
                     /translation="MKDTVDARSALKVNGREYGIWSLDSLGKDKVARLPYSLRILLEN
                     LLRHEDGVNVTRKDIQALLDWNPKAEPDTEIAFTPARVILQDFTGVPAVVDLAAMRDA
                     VVKLGGRAEAINPLSPAELVIDHSVQVDSFGAADSLKRNNEIEFGRNEERYSFLRWGQ
                     NAFDNFKVVPPNTGIVHQVNLEYLARVVFDADKDGKLIAYPDTLVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQAVTMLIPQVIGFRLKGRLAEGATATDLVLTVTEMLRKKGV
                     VGKFVEFFGDGLSNLPLADRATLGNMSPEFGSTCAIFLIDAETVRYLELSGRDAGRIA
                     LVEAYAKAQGLWREDGQPEAEYSDILELDMADVKPSIAGPKRPQDRILLSDAKQAFLK
                     HLQPIQAERADSKDAAEARFETEGGDLAVGNPASASAPGLGKVRRGGQEFVLRDGAVV
                     IAAITSCTNTSNPAVMLAAGLLARNAAARGLKVKPWVKTSLAPGSKVVTEYLQKAGLL
                     DDLETLGFNVVGYGCTTCIGNSGPLPDEIAEAITQHDIVGTSVLSGNRNFEGRIHPLV
                     RMNFLASPPLVVAYALAGNLDVDLLNEPLGDDTNGKPVYLKDIWPSSGEIQETVMGNL
                     DSGMFQGSYGSVFKGDANWQGLSVPEGEIYDWPEESTYVRNPPYFEDMGMTPASLKPI
                     AGARALAVLGDSVTTDHISPAGSIASNSPAAKYLVGQGVDPKDFNSYGSRRGNHEVMM
                     RGTFANIRLRNRLAGGVEGGWTKYLPNGEIMSIYDAAMRYQQDGTPLVILAGKEYGSG
                     SSRDWAAKGTKLLGVEAVIAESYERIHRSNLIGMGVLPLQFKDGESAASLGLDGTETF
                     TIDGLGDGDVREVTVTAARTEGKPVVFKARVRIDTPKELEYYQHGGILHYVLRQLAGA
                     DRAA"
     gene            3150..3911
                     /locus_tag="PLS34_08345"
     CDS             3150..3911
                     /locus_tag="PLS34_08345"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRP87515.1"
                     /translation="MSAAATPAPDTIPAGLLEALRQAGRVVFLTGSGVSAESGVPTFR
                     DEMTGLWARYRPEELATPEAFRRDPMLVWNWYRERRRHVAAARPNPAHHAIARLQEAL
                     PGSLLVTQNVDGLHQRAGSHDVVEFHGNLFRNRCRECGHETLHPDPELESPPACPRCG
                     HPMGPGVVWFGEAIPDDALKQAWRASAVADVFFSIGTSGLVFPAAQLVEVARWGGAVI
                     VEVNTADTALSSSADYVLRGPAGVMMPRLQQQLGS"
     gene            3959..5239
                     /locus_tag="PLS34_08350"
     CDS             3959..5239
                     /locus_tag="PLS34_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRP87516.1"
                     /translation="MTEPWASTWRGALGPGLLMAGAAVGVSHLVQSTRAGADYGLLLI
                     PVVLLACLLKYPFLEFGPRYAAATGEDLLVGYRRLGRWALGLFALVTVGTMFIVLAVV
                     TLVTAGLAGLVLGLDAPVAVISAGILAACVLLLALGNYRGLDLAMKLLMGALAVSTLS
                     AVLLAIGRQPDWSGISGVAAAPRLLTAGGVAFLLALVGWMPIPVEVAAWHSLWTRERA
                     LQTGRPPSVRHAILDFRIGFVGATVLACAFLLLGALLMHASGLRFADSAAGFSAQLVD
                     LYARNLGEWSRPLIAVAALSTMFSTTLAVADAYPRVLAAILDAARHGMDAAARPAGAG
                     RRRYLAALLIVSGGALLILHQAGAAFTRLIDFATTLSFLSAPLLAWITLRAVTGPHMP
                     PAARPGTRLLWLARLSLAFGIAFSITWLLWLLLG"
     gene            complement(5248..5988)
                     /locus_tag="PLS34_08355"
     CDS             complement(5248..5988)
                     /locus_tag="PLS34_08355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRP87517.1"
                     /translation="MSAAAEQLGLLDGADSVVVRRSARARRLALRVFPHGAVEVVAPL
                     RAGERSIREFVRSHAGWIAKARGHFRSLHGDGGFAPPAEIRLPALGERWKIEYADGPR
                     RLHAERSPDGGQLRVSGPDNDQLRRQQLRAWLKGRARETLAPWLARVSAETGLAYHSV
                     TVRRQRSRWGSCSAKHAISLNCALLFLEPPLVRHLMLHELAHTRHLDHSRAFWRLVAR
                     LDPDYEAAEQRLRAAWSDVPAWVSYDSA"
     gene            complement(5985..>6261)
                     /gene="fadH"
                     /locus_tag="PLS34_08360"
     CDS             complement(5985..>6261)
                     /gene="fadH"
                     /locus_tag="PLS34_08360"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158933.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HRP87518.1"
                     /translation="LGLQRRGVTMLPGVRYLGVEAGGLRIEVDGAERLLEVDNVVLCA
                     GQEPLDKLAGELAGSGVTVHRVGGALEAAELDAKRAIDQATRLAARL"
BASE COUNT          928 a         2235 c         2133 g          965 t
ORIGIN      
        1 cctgtttgcc acccacgcgc actggattgc cgggcgtccc ccgcgcctgc cgctggcgtg
       61 cactgcgcgc gcccgccacc ggcagccgga gcagccctgc cgggtcgagc agggcggcgc
      121 ggaaggcacg ctgcaggtcc gcttcgagca gccgcagcgg gccctgaccc ccgggcagtt
      181 catcgtcttc tatgccggcg aggagtgcct cggcggcgcg accatcgcgt cggtcggcgc
      241 gccgtttatg ccggccgccg gcgcgccccg cgccgcctcc gcctgacgcc gatccgctat
      301 aattccggat tggcaattgc cggtctgcag tactcgtcgc gccgcgccat gcagacccgc
      361 atggaccggc ccgccctcaa tccagctgga ggaatcatga aggacacggt ggacgcgcgc
      421 tccgcgctca aggtcaacgg cagggaatac ggcatctggt cgctggacag cctcggcaag
      481 gacaaggtgg cgcggctgcc gtattcgctg cgcatcctgc tggagaacct gctgcggcac
      541 gaggacggcg tcaacgtgac gcgcaaggac atccaggcgc tgctcgactg gaatccgaag
      601 gccgagcccg acaccgagat cgccttcacc ccggcgcgcg tcatcctgca ggacttcacc
      661 ggcgtgcccg ccgtcgtcga tctcgccgcg atgcgggacg ccgtggtgaa gctcggcggc
      721 cgcgccgagg ccatcaaccc gctgtcgccg gccgagctgg tcatcgacca ctccgtgcag
      781 gtcgactcct tcggtgccgc ggactcgctg aagcgcaaca acgagatcga attcggccgc
      841 aacgaggaac gctacagctt cctgcgctgg ggccagaacg ccttcgacaa cttcaaggtc
      901 gtgccgccca acaccggcat cgtgcaccag gtcaacctcg agtacctggc gcgggtcgtg
      961 ttcgacgcag acaaggacgg caaactcatc gcctaccccg acacgctggt cggcaccgat
     1021 tcccacacca ccatgatcaa cggcctcggc gtgctgggct ggggcgtcgg cggcatcgag
     1081 gccgaagccg ccatgctcgg ccaggccgtg accatgctga tcccgcaggt catcggcttc
     1141 cgtctcaagg ggcgcctggc cgagggcgcg accgccaccg acctggtgct gaccgtcacc
     1201 gaaatgctgc gcaagaaggg cgtggtcggc aagttcgtgg agttcttcgg cgacggcctg
     1261 tcgaacctgc cgctggccga ccgggccacg ctcggcaaca tgtcgccgga attcggcagc
     1321 acctgcgcga tcttcctgat cgacgccgaa acggtgcgtt acctcgagct ttccgggcgc
     1381 gatgccgggc gcatcgcgct ggtggaggcc tacgcgaagg cgcagggcct gtggcgcgag
     1441 gacggccagc cggaagccga gtacagcgac atcctcgagc tcgacatggc cgacgtgaag
     1501 ccgagcatcg ccggccccaa gcgtccccag gaccgcatcc tgctcagcga cgccaagcag
     1561 gccttcctca agcacctgca gccgatccag gcggagcgtg cggacagcaa ggacgccgcc
     1621 gaggcgcgtt tcgagaccga gggcggtgac ctcgcggtcg gcaacccggc ctccgcgtcc
     1681 gcaccgggcc tcggcaaggt gcgccgcggc ggccaggaat tcgtgctgcg cgacggcgcc
     1741 gtggtgatcg ccgcgatcac cagctgtacc aacacgtcga accccgcggt gatgctggcc
     1801 gccggcctgc tggcgcgcaa cgctgccgcc cggggcctga aggtgaagcc ctgggtcaag
     1861 acgagtctcg cgccgggatc gaaggtcgta accgagtacc tgcaaaaggc cgggctgctc
     1921 gacgacctcg agaccctggg cttcaacgtg gtcggctacg gctgcacgac ctgcatcggc
     1981 aactccgggc cgctgcccga cgagatcgcc gaggccatca cgcagcacga catcgtcggc
     2041 acctcggtgc tctccggcaa ccgcaacttc gagggtcgca tccatccgct ggtgcgcatg
     2101 aacttcctcg cctcgccgcc gctggtcgtg gcctacgcgc tggccggcaa cctcgacgtc
     2161 gacctgctga acgagccgct cggcgacgac acgaacggca agccggtgta cctcaaggat
     2221 atctggccga gctccggcga gatccaggag acggtcatgg gcaacctcga ctccggcatg
     2281 ttccagggca gctacggcag cgtgttcaag ggcgacgcca actggcaggg cctgtcggtc
     2341 cccgagggcg agatctacga ctggccggag gagtcgacct acgtccgcaa cccgccctac
     2401 ttcgaggaca tgggcatgac gccggcgtcg ctcaagccga ttgccggcgc gcgcgccctc
     2461 gccgtgctcg gcgactcggt gaccaccgac cacatttccc cggccggcag catcgcgtcc
     2521 aacagcccgg cggcgaaata cctggtgggc cagggcgtcg atccgaagga tttcaattcc
     2581 tacggctcgc ggcgcggcaa ccacgaagtg atgatgcgcg gcaccttcgc caacatccgc
     2641 ctgcgcaacc ggctggccgg cggtgtggag ggcggctgga ccaagtacct ccccaacggc
     2701 gaaatcatgt cgatctatga cgcggccatg cgttaccagc aggacggcac gccgctggtg
     2761 atcctggcgg ggaaggaata cggctccggc tcgtcgcgcg actgggcggc caagggcacc
     2821 aagctgctcg gcgtcgaggc ggtgatcgcg gaaagctacg agcgtattca ccgctccaac
     2881 ctgatcggca tgggcgtgct gccgctgcag ttcaaggacg gcgagagcgc cgcatcgctc
     2941 ggcctcgacg gcaccgagac cttcaccatc gacgggctcg gtgacggcga cgtgcgcgag
     3001 gtgacggtca ccgcggcgcg caccgagggt aagccagtgg tgttcaaggc ccgcgtccgg
     3061 atcgacacgc cgaaggagct ggagtactac cagcacggcg gcatcctgca ctacgtgctg
     3121 cgccagctgg ccggcgccga ccgggcggct tgagcgccgc ggcgacgccc gcgcccgaca
     3181 ccattccggc cggcctgctg gaggctctcc ggcaggccgg ccgcgtcgtc ttcctgaccg
     3241 gctccggcgt ctcggcggaa agcggcgtgc cgacgttccg cgacgagatg accggcctgt
     3301 gggcgcgcta caggcccgaa gaactggcga cgcccgaggc cttccggcgc gatcccatgc
     3361 tggtgtggaa ctggtaccgg gaacgtcgcc gtcacgtcgc cgcggcacga cccaaccctg
     3421 cacaccacgc catcgcacgg ctgcaggagg ccctgcccgg ctcgctgctg gtcacccaga
     3481 acgtcgacgg cctgcaccaa cgcgcgggaa gccatgacgt cgtcgagttt cacggcaacc
     3541 tgtttcgcaa ccgttgccgt gaatgcggcc atgagaccct gcaccccgac ccggagctgg
     3601 aaagcccgcc ggcctgcccg cgctgcggcc acccgatggg cccgggcgtg gtgtggttcg
     3661 gggaggccat tcctgacgac gccctgaagc aggcctggcg cgcctcagcc gtcgccgacg
     3721 tgttcttctc catcggcacc tccgggctgg tgtttcccgc cgcgcagctg gtggaggtgg
     3781 cgcgctgggg aggcgcggtg atcgtcgagg tgaataccgc ggacaccgcg ttgagctcct
     3841 cggccgacta tgtcctgcgc ggtccggcgg gcgtcatgat gccgcgcctg caacagcagc
     3901 taggttcttg aaacaacatt agaattccga cgtaactaaa tggcggggtt gagttaactt
     3961 gacggaaccc tgggcatcca cgtggcgagg cgcgcttgga ccgggcctcc tgatggccgg
     4021 cgccgccgtc ggggtctcgc acctggtgca gtcgacccgt gccggcgcgg attacggcct
     4081 gctgctgatt cccgtggtgc tcctggcctg cctgttgaaa tatcctttcc tggagttcgg
     4141 gccgcgctat gcagcggcga ccggcgagga cctgctggtc ggctaccgcc gtctcgggcg
     4201 ttgggcactg gggctctttg cgctggtgac agtcggcacg atgttcatcg tgctggccgt
     4261 cgtcaccctg gtgaccgccg ggctggcagg tctcgtgctc ggcctcgacg cgccagtcgc
     4321 cgtcatctcg gcggggatcc tggcagcctg cgtgctgttg cttgcgctgg gcaattaccg
     4381 gggcctcgac ctggcgatga agttactgat gggcgcgctg gccgtctcca cgcttagtgc
     4441 cgtgctgctg gccatcggcc gccagccgga ctggagcggc atcagcggcg tcgccgccgc
     4501 gccgcgcctg ctgacggccg gcggcgtggc gttcctgctc gccctggtgg gctggatgcc
     4561 gatccccgtc gaggtcgcgg cctggcattc gctctggacg cgggagcgag cgctgcagac
     4621 cggccggcca ccgagcgtcc ggcatgcgat tctcgacttc cgcatcggct tcgtcggcgc
     4681 caccgtgctg gcctgtgcgt tcctgcttct cggtgcgctg ctgatgcacg ccagcggcct
     4741 gcgtttcgcg gattccgccg cgggtttttc cgcgcagctg gtggacctct atgcgcgcaa
     4801 cctcggcgaa tggagccggc cgctgatcgc ggtcgccgcg ctcagcacca tgttcagcac
     4861 cacgctcgcg gtggcggacg cctacccgcg cgtgctggcg gcgatcctgg acgcagcgcg
     4921 ccacgggatg gatgccgcgg cgcgcccggc cggcgcgggc cggcgacgct atcttgcggc
     4981 gctgctcatt gtcagcggcg gtgcactgct gatcctccac caggcaggtg ctgccttcac
     5041 gcggctgatc gatttcgcga ccaccctttc cttcctgtcc gcccccctgt tggcctggat
     5101 caccttgcgt gcagtgaccg ggccccacat gccgccggcc gcacgaccgg gaacgcggtt
     5161 gctgtggttg gcacggctca gcctggcatt cgggatcgct ttctcgatca cctggctgct
     5221 ctggctgctg ctcggctgac gacgggctca ggccgagtcg tagctcaccc aggccggcac
     5281 atcgctccag gctgcgcgca ggcgctgctc ggcggcctcg tagtccgggt ccaggcgcgc
     5341 caccagtcgc cagaaggcgc gcgagtggtc caggtgccgg gtgtgcgcca gctcgtgcag
     5401 catgaggtgg cgcaccagcg gcggctcgag gaacaacagc gcgcagttga ggctgatggc
     5461 gtgcttcgcg gaacagctgc cccagcgaga ccgctgccgc cgcacggtga cgctgtggta
     5521 ggcgagcccg gtctcggcac tgacgcgcgc cagccagggc gccagggtct cccgcgcccg
     5581 ccccttgagc caggcgcgca gctgctgcct gcgcagctgg tcgttgtcgg gtccgctgac
     5641 ccgcagttgg ccaccatcgg gcgagcgctc ggcgtgcagc cgccgcgggc cgtcggcata
     5701 ctcgatcttc cagcgttccc cgagcgccgg caaccggatc tctgcaggtg gcgcgaagcc
     5761 accgtcgccg tgcagcgagc ggaagtgtcc gcgcgccttc gcgatccagc cggcgtggct
     5821 gcgcacgaac tcacggatgc tgcgctcgcc ggcgcgcagc ggtgcgacga cctccactgc
     5881 gccgtgcgga aacaccctga gcgccagccg gcgggcccgc gcgctgcgcc ggaccactac
     5941 ggaatccgcg ccgtccagca ggccgagctg ttccgcggcc gcgctcacag gcgggcggcg
     6001 agccgggtcg cctggtcgat ggcgcgcttg gcgtcgagtt cggccgcctc gagcgcaccg
     6061 cccacccgat ggaccgtcac gccactgccc gccagctcgc ccgccagctt gtcgagcggc
     6121 tcctggcccg cgcacagcac gacgttgtcc acctcgagca ggcgttcagc accgtcgacc
     6181 tcgatacgca agccgccggc ttcgacaccg agatagcgga cgccgggaag catcgtcacg
     6241 ccgcggcgtt gcagtccgag c
//
LOCUS       DAOMPG010000140         6174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5423_length_6174_cov_4.351528, whole genome shotgun sequence.
ACCESSION   DAOMPG010000140 DAOMPG010000000
VERSION     DAOMPG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5423_length_6174_cov_4.351528"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLS34_08365"
     CDS             complement(<1..511)
                     /locus_tag="PLS34_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRP87519.1"
                     /translation="MPSRALKFLPIVHWIRLTTRRDLGADVTAGAITAVLLVPQGLAY
                     AMIAGLPPVVGLYASILPAILYAVFGTSRDLSVGPTSIAAIMVAAAVTGAGAGIDPVA
                     TAVILAALSGLILLAMGILRMGVFANLLSQPVLTGFSSGAAIVIMADQLRHFAGIELP
                     AGLQPHEMLA"
     gene            complement(575..1111)
                     /locus_tag="PLS34_08370"
     CDS             complement(575..1111)
                     /locus_tag="PLS34_08370"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRP87520.1"
                     /translation="MTRRLPLLLAALLGLTLLAQSPAHATPPEGAAAPAFELPDQQGE
                     LRRLGDYDGKWLVLYFYPKDDTPGCTTQACEFRDNIFAFRRAGAEIVGISLDDVESHM
                     AFAEKHGLPFTLLADEGGVVAREYGVLRNFGVVTLASRQTFLISPDGRIARHYAKVDV
                     NTHADEVLRDIAALSGGG"
     gene            complement(1164..2069)
                     /gene="rapZ"
                     /locus_tag="PLS34_08375"
     CDS             complement(1164..2069)
                     /gene="rapZ"
                     /locus_tag="PLS34_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRP87521.1"
                     /translation="MELVVVSGLSGSGKSVALAMLEDLEWYTLDNVPAHLLSLVVGEL
                     TKDEDPKFNRLAIGLDSRPRPADLPMIRHLLEDLRARGVRCQLVFLHASETALVHRYL
                     DTRRRHPHTPGKSGDRTLADAIKFEQALLTPLAETADLVLDTSATSVHELRDIVRDRV
                     AGQPRNQVSLQFESFGFRQGIPKDADFVFDARFLPNPYWEPELRMLSGRDQGVIDYLS
                     ASPEVQAYLEDLKRFLSSWIDRIEAANRSYLTVAIGCTGGRHRSVYLAERLARHFQDD
                     FPGVTVRHTGLEQLAAANARGGTNP"
     gene            complement(2089..2565)
                     /locus_tag="PLS34_08380"
     CDS             complement(2089..2565)
                     /locus_tag="PLS34_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRP87522.1"
                     /translation="MTFASLLKPGRVLSNVEARSKKHALDILSELLASAADLDQGDIF
                     QSLISREKLGCTAIENGIAIPHGRLEELEAPVGAFLKLSRPVDFDMPDREPVDLIFGL
                     LVPMNGDDVGCHHEEVSLIATALDDRALAAALRRATSSRSLYDLLVNHEASRAASA"
     gene            complement(2599..2916)
                     /gene="raiA"
                     /locus_tag="PLS34_08385"
     CDS             complement(2599..2916)
                     /gene="raiA"
                     /locus_tag="PLS34_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRP87523.1"
                     /translation="MQINLTGHHVDITDPLRDYVHNKMDRVVRHFDNLIDVHCILTVE
                     KLQHKAEATLRVAGDTLHATATEEDMYAAIDGMIDRLDRQVRRHKDKLTDHHNGAGRP
                     RPA"
     gene            complement(3042..4493)
                     /locus_tag="PLS34_08390"
     CDS             complement(3042..4493)
                     /locus_tag="PLS34_08390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRP87524.1"
                     /translation="MKQSLQLKLGQQLTLTPQLQMAIRLLQLPVLDLQAELREALEKN
                     LMLEMDDGLELAPPKGEQQAPSEAHQESAPDTPGEDRAEGQEDYREDLPYADVTEFGA
                     AYGGPGRDDAEDQRDYADVSGSSLRDHLLAQVDVEFPEGDRKFIATMLVDAIDDDGYL
                     TETLEEICAHLAPDIETEVDEVERVLARIQRFDPLGVGARDLSECLLLQLAPFAPDTP
                     GLDLVRRLAADCLAELGEQEYGVIRRRLDCSAEELEHAVALLRSLNPRPGSAADTRPP
                     EYIVPDVFVRRGDESWRVEINPAIAPRLRVNTAYAGCLGRSGEYSTLRTQLQEARWLV
                     KSLEIRNETLIRVARAIVSHQQDFLERGEEGMRPLVLREIAEALELHESTVSRATTGK
                     YMHTPRGVFEFRYFFSSQVAGSDGESVSSTAIRARIRKLISDEDPQKPLSDSALTRML
                     MSEGIEVARRTVAKYRETMGFPSSNERRRVAIR"
     gene            complement(4513..5238)
                     /gene="lptB"
                     /locus_tag="PLS34_08395"
     CDS             complement(4513..5238)
                     /gene="lptB"
                     /locus_tag="PLS34_08395"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007990185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRP87525.1"
                     /translation="MRTLRAEGIAKSYRSRQVVRSLSLSISSGEVVGLLGPNGAGKTT
                     AFYMIVGLVPCDSGRILLDDADLTALPMHRRARLGVGYLPQEASVFRGLTVEQNVLAV
                     LETRKELDALGRRQALEELLEELNIAHIRDSKGLSLSGGERRRVEIARALAAEPAFIL
                     LDEPFAGVDPISVLDIQRIIGHLRDRGIGVLITDHNVRETLGICGRAFILNAGEVIAS
                     GSADEILANRQVREVYLGEHFRL"
     gene            complement(5235..5873)
                     /locus_tag="PLS34_08400"
     CDS             complement(5235..5873)
                     /locus_tag="PLS34_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRP87526.1"
                     /translation="MVASSPDCRSRRAPPRLAPALLGLVALFGVPPLVAQQPQQEITL
                     EAESSDFDRRNERLLFRQVRIQQGDTVVSADQAESRDLDFSQGTWLFQGNVLIRSPMG
                     DIESARATISFAGHRLTRAAADGAPARFSRTMPPPEARVVTASANRIVYDLGRDELEL
                     AGQAVLRDGLREVSGGRLLYRISEDRLIASADDDGSDRVRIVITPPETEENP"
     gene            5941..>6174
                     /locus_tag="PLS34_08405"
     CDS             5941..>6174
                     /locus_tag="PLS34_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87527.1"
                     /translation="MLSQRQLDQDRGARDVRGGVDLEALGFDRGVLVQGGAHGTDNAA
                     MLDALQRLGPRVKGIAVLPPGTPLREREALHERG"
BASE COUNT          954 a         2047 c         2182 g          991 t
ORIGIN      
        1 aggccagcat ttcgtgcggc tgcaggccgg ccggcagctc gatgccggca aaatgtcgca
       61 gctggtcggc catgatcacg attgccgcgc cgctggagaa gccggtcaac accggctggc
      121 tcagcaagtt ggcgaacacg cccatccgga ggatgcccat ggcgagcagg atgagacccg
      181 acagggcggc aaggatcact gcggtggcca ccgggtcgat gccggcgccg gcgccggtga
      241 cggccgccgc gaccatgatc gcggcgatcg aggtcgggcc cacggacagg tcccggctgg
      301 taccgaacac ggcgtacagg atggcgggca ggatggaggc gtagagcccg accaccggtg
      361 gcaggcccgc gatcatggcg tacgccagcc cctgcggcac cagcagcacc gcggtgatgg
      421 ctcctgcggt cacgtccgcg ccgaggtccc ggcgcgtggt cagccggatc cagtgcacga
      481 tcggcagaaa cttgagagct cgcgaaggca atgtggcagg cctgttcaat gcaggcctgc
      541 aagatagtga agcgccccgt ggagggtcaa gcgatcagcc gcccccggac agcgccgcga
      601 tgtcccgcag cacctcgtcg gcgtgagtgt tcacgtcgac cttggcgtaa tgacgcgcaa
      661 tccggccgtc cggcgaaatc aggaatgtct gccggctggc cagcgtgacg acgccgaaat
      721 tgcgcagcac gccgtactcg cgcgccacca cgccaccctc gtcggcgagc agcgtgaacg
      781 gcaggccgtg cttttcggcg aaggccatgt gcgactccac gtcgtcgagg ctgatgccga
      841 cgatctccgc accggcacgg cggaatgcaa agatgttgtc gcggaattcg caggcctggg
      901 tggtgcagcc gggcgtgtcg tccttcgggt agaaatagag caccagccac ttgccgtcgt
      961 agtcgccgag acgccgcagc tcaccttgct ggtccggcag ctcgaaggcc ggtgcagcag
     1021 cgccttcggg cggcgtggca tgtgccggcg attgcgccag cagggtgagg cccagcaggg
     1081 cggcaaggag aagcgggagt ctgcgtgtca tggtcgtgtc ctgctggtgg ggagctcgga
     1141 aagcggattc gcctcgcccg cggctacgga ttcgtgccgc cccgggcgtt cgcggccgcc
     1201 agttgctcga ggcccgtgtg gcgcaccgtc acgccgggga agtcgtcctg gaaatgccgg
     1261 gcgagccgtt cggccaggta gacggagcga tggcgcccgc cggtgcagcc gatggcgacc
     1321 gtcaggtagc tgcggttggc ggcctcgatc ctgtcgatcc acgaagacag gaagcgcttg
     1381 agatcctcca ggtaggcctg cacctccggg ctggccgaca ggtagtcgat gacgccctgg
     1441 tcgcggcccg acagcatgcg cagttcgggc tcccagtagg gattcggcag gaagcgggcg
     1501 tcgaacacga agtccgcgtc cttcggtata ccctggcgga agccgaagga ctcgaactgc
     1561 agcgagacct ggttgcgcgg ctggccggcg acacggtcac ggacgatgtc gcgcaattcg
     1621 tggacgctgg tcgcgctggt atccagcacc aggtcggcgg tctcggccag cggcgtgagc
     1681 agcgcctgtt cgaacttgat ggcatcggcc agggtccggt cgccgctctt gcccggggtg
     1741 tgcggatgcc ggcgccgcgt gtcgaggtag cggtgcacca gcgcggtttc cgaggcgtgc
     1801 aggaagacca gctggcaacg cacgccgcgc gcccgcaggt cttccagcag gtggcgtatc
     1861 atcggcaggt ccgcgggacg cggccgcgaa tcgaggccga tggcgaggcg gttgaacttg
     1921 gggtcctcgt ccttggtgag ttccccgacc accagcgaca gcaggtgcgc cggcacgttg
     1981 tccagcgtgt accactcgag atcctcgagc atggccagcg ccacgctctt gccggatccc
     2041 gacaggccgc tgacgactac cagttccatc gccggcggtc gccccgcgtc acgccgaggc
     2101 cgcgcgggag gcttcgtggt tgaccagcag gtcgtagaga gaccggctcg aggttgcgcg
     2161 tcgtaacgcc gcggccagcg cgcgatcgtc gagcgccgtc gcgatcagcg agacttcctc
     2221 gtggtggcag ccgacgtcgt ccccgttcat cggcaccagc aggccgaaaa tcaggtcgac
     2281 cggctcgcgg tccggcatgt cgaaatcgac cggccgggaa agcttgagaa acgcccccac
     2341 gggcgcctcg agctcctcga ggcgcccgtg gggaatggcg atgccgttct ctatggccgt
     2401 gcagccgagt ttctcgcggc tgatcaggct ctggaagata tcgccctggt ccaggtccgc
     2461 ggccgacgcc agcagctcgc tgaggatgtc gagagcgtgc ttcttgctgc gcgcctcgac
     2521 gttgctcagc acgcggccgg gtttgagcag ggatgcgaaa gtcattggat cgctccgggc
     2581 ggccatcgcc cgcgggtttc aggcggggcg cgggcgcccg gcaccgttgt ggtggtcggt
     2641 cagcttgtcc ttgtggcgcc gcacctggcg gtcgagccga tcgatcatgc cgtcgatggc
     2701 ggcgtacatg tcctcttccg tcgccgtggc gtgcaaggtg tcgcccgcga cgcgcagcgt
     2761 ggcctcggcc ttgtgctgga gtttctcgac cgtcaggatg caatgcacgt cgatgaggtt
     2821 gtcgaaatgg cgcacgaccc ggtccatctt gttgtgcacg tagtcgcgca gcgggtcggt
     2881 gatgtcgacg tgatggccgg tcaggttaat ctgcatttgt tcgctcctcg ggagtttctc
     2941 gggccggtcc gtcgggcggc ctgctccatg gttctattct agtagccccc gtccgtggct
     3001 ggctagggag ccggcagcgg ggcaccgccg gccggcaata ttcaacgaat tgcgacccgc
     3061 cgccgctcgt tggaggacgg gaaacccatc gtttcccggt acttggcgac ggtacgccgc
     3121 gccacctcga tgccctccga cattagcatg cgggtcaggg cgctgtccga cagcggtttc
     3181 tggggatctt catcggaaat gagcttacgg atacgcgccc ggatggccgt cgacgagacg
     3241 ctttccccgt cgctgccggc gacctggctg gagaagaagt agcggaactc gaacaccccg
     3301 cgcggcgtat gcatgtactt gccggtggtg gcccgggaca cggtcgactc gtgcaactcc
     3361 agcgcctcgg cgatttcgcg cagcaccagc ggccgcatgc cctcctcgcc gcgctcgagg
     3421 aagtcctgct ggtgggacac gatggcgcgg gccacccgga tcagggtttc gttgcggatc
     3481 tcgaggctct tcaccagcca gcgcgcttcc tgcagctggg tgcgcagggt ggagtattcg
     3541 ccgctccggc cgagacagcc ggcgtacgcc gtgttcactc tcaggcgggg cgcgatcgcc
     3601 gggttgattt ccacccgcca gctctcgtcg ccgcgccgca cgaacacgtc cgggacgatg
     3661 tactcgggcg ggcgcgtgtc ggccgcgctg ccggggcggg gattgagcga gcgcagcagg
     3721 gccaccgcgt gctcgagctc ctcggcgctg cagtccaggc gacgccggat gacgccgtac
     3781 tcctgctcgc cgagctccgc caggcagtcg gccgcgaggc gccgcaccag gtcgaggccg
     3841 ggcgtgtccg gcgcgaacgg ggcgagctgg agcagcaggc attccgacag gtcgcgcgcg
     3901 ccgacaccca gcgggtcgaa gcgctggatc cgggcgagca cacgctccac ctcgtcgact
     3961 tcggtttcga tatcgggcgc caggtgggca cagatctcct cgagcgtctc ggtcaggtag
     4021 ccgtcgtcgt cgatggcgtc caccagcatg gtggcgatga acttgcgatc accctcggga
     4081 aactcgacgt ccacctgcgc gagcaggtgg tcgcgcaggg agctgccgct gacatcagcg
     4141 tagtcgcgct ggtcctcggc gtcgtcacgc ccgggacccc cgtacgcggc gccgaactcc
     4201 gtgacgtcgg cgtagggcag atcctcgcgg taatcctcct ggccctcggc gcggtcctcg
     4261 cccggggtat ccggcgcgga ttcctggtgg gcctccgacg gcgcctgctg ctcacccttc
     4321 ggcggtgcca gctcgaggcc gtcgtccatc tcgagcatga ggttcttctc gagcgcctcg
     4381 cgcagctccg cctgcaggtc gagcacgggc agctgcaaca gccggatggc catctgcagc
     4441 tgaggcgtca gcgtcagctg ttgaccgagc ttcagttgca gcgattgctt catcatgggg
     4501 ctgacattct gctcacaagc ggaagtgttc gccgagatac acttcgcgaa cctgccggtt
     4561 ggccagtatc tcatccgccg agccggaggc gatgacctcg ccggcattga gaatgaacgc
     4621 ccgcccgcag atgcccagcg tctcacgcac gttgtgatcc gtgatcagca cgccgatccc
     4681 gcggtcgcgg agatggccga tgatccgctg gatgtcgagc accgagatcg gatcgacccc
     4741 ggcaaagggc tcgtccagca ggatgaaggc gggctcggcc gccagcgcgc gggcgatctc
     4801 gacgcggcgg cgctcaccgc cggacaggct cagccccttg ctgtcacgta tgtgggcgat
     4861 gttcagctcc tcgagcaact cctccagcgc ctggcgccgt cccagcgcgt cgagttcctt
     4921 gcgcgtctcc agcaccgcga gaacgttctg ctcgacggtc aacccgcgga acacggaagc
     4981 ctcctgcggc aggtagccga cgccgagccg cgcccgccgg tgcatcggca gcgcggtcag
     5041 gtcggcgtcg tccagcagga tgcggccgct gtcacacggc accaggccca cgatcatgta
     5101 gaaggccgtg gtcttgccgg cgccgttcgg gccgagcagg ccgaccacct cgccgctgga
     5161 gatcgacagc gacagcgacc ggacgacctg gcgggaccgg tagcttttcg caatgccttc
     5221 ggcgcgcagg gtcctcacgg attctcttcc gtctcgggcg gtgtgatgac gatccggacg
     5281 cggtccgacc cgtcgtcgtc tgcgctggcg atcagccggt cctcggagat gcggtacagc
     5341 agtcgcccgc cgctcacctc gcgaagaccg tcgcgcaata ccgcctggcc ggccagttcg
     5401 agctcgtccc ggccgaggtc gtagacgatc cggttcgccg aggcggtcac cacccgggcc
     5461 tcggggggcg gcatggtgcg cgaaaagcgg gccggggcgc cgtcggccgc ggcccgcgtg
     5521 agccggtgac cggcaaagct gatggtcgcg cgggcggact cgatgtcgcc catggggctg
     5581 cgaatcagga cattgccctg gaacagccag gtgccctggg agaagtcgag gtcgcgcgac
     5641 tcagcctggt cggccgagac cacggtgtcg ccctgctgga tccgcacctg gcggaacagc
     5701 aaccgctcgt tgcgacgatc gaagtcggaa gattcggcct cgagggtgat ctcctgctgg
     5761 ggctgttgcg cgaccagcgg cggcacgccg aacagcgcca cgagaccgag cagcgcaggc
     5821 gcaagccgcg gcggagcgcg ccgggaacgg cagtcagggg aggaagcgac catggacttc
     5881 ggattgtaac tgcagccgtt cctccagcaa gtaagcgacc atgcccacgc cgctgagccg
     5941 ttgctctccc agcgccagct ggaccaggac cgcggagcgc gcgacgtgcg tggcggtgtc
     6001 gatctcgagg cgctcggttt cgaccgcggc gtcctggtcc agggcggcgc tcacggcacc
     6061 gacaacgccg ccatgctgga cgccctgcaa cggctgggcc cgcgggtgaa gggcattgcg
     6121 gtgctgccgc ccggcacgcc gctgcgcgag cgcgaggcct tgcatgagcg cggc
//
LOCUS       DAOMPG010000141         6148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5450_length_6148_cov_4.546857, whole genome shotgun sequence.
ACCESSION   DAOMPG010000141 DAOMPG010000000
VERSION     DAOMPG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_6148_cov_4.546857"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PLS34_08410"
     CDS             <1..1311
                     /locus_tag="PLS34_08410"
                     /inference="COORDINATES: protein motif:HMM:NF026213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRP87528.1"
                     /translation="GPDAFPNLLSFTPEKFLVASSYQPPTKLFADGKVVELVIAGEPI
                     QVAPSRTDTTDSVAIYFPQRRLMVTNFLVIDTIFNIYTLRGGAFRNPEILVDDARWVE
                     SRNAEILLDIHGPTLRGEEVVREALERSVDSVQLIHDQTLRLVASGLGPREAAETIYV
                     PRNLREDREGYGQVESHVRQVYNGNIGWFDGDVYQINPLALKEEAERMVQAMGGRAKV
                     QEMASRAVADGGLANWRWGLKLTSLLLRLDPADPAARQTRATAARALGQRTDSANARG
                     FYITEALQMEGNLLVQGQPQTMDGIRTFLNTPRLDRLAAVSVDQNLQFVRYLIDPGKA
                     EGKRLAFTIAAEGDPQIRRVELRNGVLVILPVDSVGARHVDVTRSELADFVVGKGAPA
                     RAGGELLAELDRVLDRSRLMPPTMTKPAVLDDKGDRDYNHGLEH"
     gene            1347..3200
                     /locus_tag="PLS34_08415"
     CDS             1347..3200
                     /locus_tag="PLS34_08415"
                     /inference="COORDINATES: protein motif:HMM:NF026213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRP87529.1"
                     /translation="MLNILACAVIVLAASPGSPAQAAASADGERSWHSPSLRTETVTG
                     PNGAVTTQAALLANDKWGIGDTKRVEQVTDGVYAMRGWGIASSFAIKAPEGWIIIDTG
                     DHTPAATEMRAMLEESLGGPIRVAAILLTHWHYGNGIGAWLDEGTEVWGHEHLDRNLR
                     RSTGISVLSGTYQARAAAQFGVFHPQNGPDAFPNTLGFTPDKLLAGSSYQPPTRLFPD
                     GRVIDVVVAGEPVQVAPNRSDANDSVGFYFARKRMLVTNFMVPGGIFNVYSLRGGSYR
                     NPAVFVEDGRWIESKNAEILLDIHGPTVKGEDAVRNAVQRALDQVQLIHDQTLRMIAR
                     GVDARRAAEEVYMPQHLRGDWENYGQVESHVRQVFNGTMGWFGGDVADINPLSVNEEA
                     VRTVQMMGGQAAVRKAAAEANAAGGLTDWRWTLTLTSLLLELDPADSEALELRASAAR
                     QLGQRTSSANARGWYLSEALQIEGGLQFKGQPLTFDVIRRFLGTPAAQALAAASVDEN
                     LQFVRYLVDPRQAEAQRLMFTLAVEGDPRIRQVELRNAVIVISDVDAEAPVHLSLTRQ
                     ELADFVLGTRPASAADPLTELDRVLDRSHLMPPGAVESALQGVKAASYLEH"
     gene            3319..3392
                     /locus_tag="PLS34_08420"
     tRNA            3319..3392
                     /locus_tag="PLS34_08420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3351..3353,aa:Gly,seq:ccc)
     gene            complement(3493..3984)
                     /locus_tag="PLS34_08425"
     CDS             complement(3493..3984)
                     /locus_tag="PLS34_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87530.1"
                     /translation="MSKFAIAFLFTLTFMLSIAGCSKEPATPAASAPEPAASSTPAQP
                     PHPMPAAPSEAVDLSGIEKADGGKTIAEVFAGKDQLAGQTIVFRGKVVKVNANIMGKN
                     WLHVRDGTGEEGTNDLTVTTADVLPEVGDTVLVTGTVAINKDFGMGYQYAILVEDAEV
                     VVE"
     gene            complement(4153..5829)
                     /locus_tag="PLS34_08430"
     CDS             complement(4153..5829)
                     /locus_tag="PLS34_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HRP87531.1"
                     /translation="MRLTRFLTIAAVAALGAGLAGCDGDDGAAGPAGATGPAGPSGPA
                     GTGEALLDLNLIGRYSSGIFDASAAEIVAFDPATQRLFVVNAAAVTIDVLNLADPANP
                     VLLDTIDASSEGGGANSVAVHGGVAAVAIEANVKTDPGKVVFYDTSTLAKISEVEVGA
                     LPDMLTFTPDGNAVLVANEGEPNEGYTIDPEGSVSVIDVSGGFDAPTVATAGFAAFNA
                     SIDALRAAGVRIYGPGATVAQDLEPEYIAVAPDGLSARVVLQEANALAVLDLADISAP
                     EITEIMPLGVKDHMILGNELDPSDRDPQGDPQIRIRNWPVYGMYQPDAIAAYQFNGKT
                     YYVTANEGDDRDDFIPGEETARVSSLTLDPVAFPNAAELQAASALGRLTVSTYDGDLD
                     DDGQYEKLFVLGARSFSIWAEDGTQMYDSGSDFERITAQRFPQNFNASNSSNERENRS
                     DNKGPEPEGVVLGELAGRTFAFSGLERIGGIMVYDVTNPQRASFVQYVNSRDFSKDPE
                     SELALVGDLGPEGLLFIPVADSPSGEPLLVVGNEVSGTTSIFQVDVITLP"
     gene            complement(5892..6116)
                     /locus_tag="PLS34_08435"
     CDS             complement(5892..6116)
                     /locus_tag="PLS34_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87532.1"
                     /translation="MKVVTWELLEGLGLCVLALGFGCVMLYGTFVAVGQSAHLRAAVF
                     GILALFALYWGVRLTLAELARYRERSGRIS"
BASE COUNT         1045 a         2035 c         2071 g          997 t
ORIGIN      
        1 ggccccgacg cgtttccgaa cctgctcagc ttcactcccg agaagttcct ggtggcgtcc
       61 agctaccagc cgccgacgaa gctcttcgcg gacggcaagg tcgtcgagct ggtcatcgcc
      121 ggcgagccga tccaggtcgc gccgagccgc acggacacga cggacagcgt cgcgatctac
      181 ttcccgcaac gccggctgat ggtgaccaac ttcctggtga tcgacaccat cttcaacatc
      241 tacacgctgc gcggcggcgc cttccggaat cccgagatcc tggtggacga cgcgcgctgg
      301 gtcgagtcca ggaatgccga gatcctgctc gacatccacg gtccgacgct ccggggggag
      361 gaggtggtgc gggaagcgct ggagcgctcc gtcgactcgg tgcagctgat ccatgaccag
      421 acgctgcgcc tggtcgcgag cggcctgggt ccccgcgagg cggcagagac gatctacgtg
      481 ccccgcaacc tgcgcgagga ccgtgaaggc tacggccagg tcgagagcca cgtacggcag
      541 gtctacaacg ggaacatcgg ctggttcgac ggcgacgtct accagatcaa cccgctcgcg
      601 ctcaaagaag aagccgagcg gatggtgcag gcgatgggcg gccgcgcgaa ggtgcaggaa
      661 atggcctcgc gggcggtggc cgacggcggg ctcgcgaact ggcgctgggg cctcaagctc
      721 acctcgctgc tgctccggct cgatcccgcg gacccggccg cgcggcagac tcgtgcgacg
      781 gcggcgcgtg ccctcggcca gcgcaccgac tcggccaacg cgcgggggtt ctacatcacc
      841 gaagcgctgc agatggaggg caaccttctc gtgcagggac agccgcagac aatggacggg
      901 atccgcacgt tcctcaacac accgcgactc gatcgcctgg cggcggtttc tgtcgaccag
      961 aacctgcagt tcgtccgcta cctcattgac cccggcaagg cggaaggcaa gcgattagcg
     1021 ttcaccatcg cggccgaggg cgacccgcag atccggcgag tcgagctgcg caacggcgtg
     1081 ctcgtcatct tgccggtcga ctctgtcggc gcccgccacg tcgacgtgac gcgcagcgag
     1141 ctggcggatt tcgtcgtcgg caagggtgcg ccggcgaggg ccggcgggga actgctggcc
     1201 gagctggatc gcgtgctcga ccgcagccgg ctgatgccgc cgacgatgac caagccggca
     1261 gtgctcgacg acaagggcga ccgcgattac aaccacggcc tcgaacacta actgaaccga
     1321 gagaaaagcc ggctatgaaa atcagtatgc tcaacatcct ggcctgcgca gtcatcgtct
     1381 tggccgcgtc ccccggctcc ccggcccaag ctgcggccag tgccgacggc gagcgttcct
     1441 ggcacagccc gtccctgcgc actgaaaccg tcaccggccc caacggcgcg gtgacgaccc
     1501 aggccgcgct gcttgccaac gacaaatggg gcatcggcga caccaagcgc gtcgagcaag
     1561 tgaccgacgg cgtgtacgcg atgcgcggtt ggggcatcgc gagcagtttc gcgatcaaag
     1621 ctcccgaggg ttggatcatc atcgacaccg gcgaccacac gccggccgcc accgagatgc
     1681 gcgccatgct cgaggagtcg ctcggcgggc caatcagggt ggccgcgatc ctcctgaccc
     1741 actggcacta tggcaacgga atcggcgcat ggctcgacga agggacggag gtctggggac
     1801 acgagcatct ggaccgcaac ctcagacgct cgaccggcat cagcgtcctg agcggcacct
     1861 accaggcacg agcggccgcc cagttcggcg tcttccatcc acaaaacggt cccgatgcct
     1921 ttccgaacac gctcggcttc acgcccgaca aactgctggc cgggtccagc taccagccgc
     1981 ccacgcgtct cttcccggac ggtcgcgtca tagacgtcgt cgtcgccggc gagccggtgc
     2041 aggtcgctcc caatcgctcg gacgctaacg acagcgtcgg cttctatttc gcgcgcaagc
     2101 gcatgctcgt gaccaacttc atggtgccgg gcggcatctt caacgtgtac tcgctgcgcg
     2161 gtggttcgta tcgcaacccg gcggtgtttg ttgaggacgg ccgctggatc gagtcgaaga
     2221 acgcggaaat cctgctcgac attcacggcc caaccgtcaa gggcgaagat gcggtccgca
     2281 atgcggtcca gcgtgccctt gaccaggtgc aacttattca cgaccagacg ctgcgcatga
     2341 ttgcccgcgg ggtggatgcg cgtagggccg ccgaggaggt gtatatgccg cagcacctgc
     2401 gcggcgactg ggagaactac gggcaggtag aaagccacgt ccggcaggtc ttcaatggca
     2461 cgatgggctg gttcggcggc gacgtcgccg acatcaatcc gctgtccgtc aacgaggaag
     2521 ccgtgcgtac cgtccagatg atgggtggcc aggccgccgt ccgcaaagcc gccgcggaag
     2581 ccaacgccgc gggcggactc accgactggc ggtggaccct caccctgaca tccctgctgc
     2641 tggaactcga tccggctgac tccgaggcgc tcgagctccg ggcctcggcg gcgcgccagc
     2701 tcggccagcg gacctcatcg gccaacgcgc gcggctggta cctgtcggag gcgctgcaga
     2761 tcgaaggcgg cttgcagttc aagggccagc cgctgacctt cgacgtgatt cgccgtttcc
     2821 tcggcacccc cgccgcgcag gcactcgcag cggcctccgt ggatgagaac ctgcagttcg
     2881 tgcgctacct cgtcgatccg cgccaggccg aggcgcagcg actgatgttc acgctcgctg
     2941 tcgaaggcga cccgcgcatc cggcaggttg agttgcgcaa tgccgtcatc gtgatctcag
     3001 acgtggacgc cgaggcaccc gtgcatctgt cactgacgcg acaggagctc gccgattttg
     3061 tgctcggcac gcgcccggcg tccgccgccg acccgctgac cgaactcgac cgggtccttg
     3121 accgtagcca cctgatgccc cccggggcgg tggagtcggc gctgcaggga gtgaaggcgg
     3181 ccagctatct cgagcattga gccaaaccag cctgcgagcg gttcccgccg gcactggcca
     3241 gcaggccagg cctggcggcg cgcttgcagg aatggattag ggccgccgga gccagtagaa
     3301 tctcgaacgc tacgtcgggc gggtgtagtt caatggtaga acagtagctt cccaagctac
     3361 gaacgtgggt tcgattccca tcacccgctc caggacatga gaagcccccg cttgtcgggg
     3421 gcttcgtcga tctggacgtg ggtgacggga ttagaagcca cgtccctcgc acggcggagc
     3481 ggtgcgaggt ggctactcca ccaccacctc ggcgtcctcg acgaggatgg cgtactggta
     3541 gcccatgccg aaatccttgt tgatcgcaac cgtgccggtc acgagcacgg tgtcgccgac
     3601 ctcaggcagg acgtcggccg tggtgacggt gaggtcgttc gtgccttcct cgccggtgcc
     3661 gtcgcggaca tgcagccagt tcttgcccat gatgttggcg ttgaccttga ctaccttgcc
     3721 ccggaacacg atcgtctggc ctgccaactg gtccttcccg gcgaacactt cggcgatggt
     3781 cttgccgccg tccgccttct cgatgccgga caggtcgacc gcctcgctgg gcgcagcggg
     3841 catggggtgg ggcggctggg ccggcgtgct ggaggcggcc ggctcgggcg cgctcgcggc
     3901 gggagtcgcc ggctccttgc tgcagcccgc aatggagagc atgaaggtaa gggtgaacag
     3961 gaaggcaatg gcgaactttg acacgggaag atactccgga ttgggcggac acgacggccg
     4021 cggtgggcac catcgttcac ggaaatgagg gcgtattgta cccgacgacg gctggcgcca
     4081 ccgtaagtag tatcgcagac gaaaaagggc gctcccttga gggagcgccc cggtcgaggt
     4141 cgggacgggc ggttaaggca gcgtgatgac gtcaacctgg aaaatggacg tcgtaccgct
     4201 gacctcgttg cccactacca gcagcggctc gccgctgggg ctgtcggcta cgggtatgaa
     4261 cagcaggccc tccgggccga ggtcgccgac cagcgccagt tcgctttcag ggtccttgct
     4321 gaagtcacgt gagtttacgt actggacgaa gctcgcgcgc tgcgggttgg tcacgtcgta
     4381 gaccatgatg ccgccgatgc gctcgaggcc gctgaaggcg aaagtgcgac cagccagttc
     4441 cccgagcacc acgccctccg gctcggggcc cttgttgtcg ctgcggtttt cgcgctcgtt
     4501 gctgctgttg ctggcgttga agttttgcgg gaagcgctgc gccgtgatgc gttcgaagtc
     4561 ggaaccgctg tcgtacatct gggtaccgtc ctccgcccag atggagaacg agcgggcgcc
     4621 cagcacgaac agcttctcgt actgcccgtc atcgtccagg tcgccgtcgt aggtcgagac
     4681 cgtgagccgg ccgagcgccg aggcggcctg cagctccgca gcattcggga aggcgaccgg
     4741 gtcgagcgtc agcgagctga cgcgcgccgt ttcctcgccc gggatgaagt cgtcgcggtc
     4801 gtcgccctcg ttggcggtga cgtagtaggt cttgccgttg aactggtagg ccgcgatcgc
     4861 gtccggctgg tacatgccgt aaaccggcca gttgcggatc cggatctgcg gatcgccctg
     4921 cgggtcacgg tcactgggat ccagctcgtt gccaaggatc atgtggtcct tgacgcccag
     4981 cggcatgatc tcggtgatct ccggcgccga gatgtccgcc aggtcaagca ccgccagcgc
     5041 gttggcctcc tgtagcacga cgcgcgccga caggccgtcg ggagccaccg cgatgtactc
     5101 gggctcaagg tcctgggcaa ccgtggcgcc gggaccgtag atgcgcacgc cggccgcgcg
     5161 cagggcgtca atgctggcgt tgaacgcggc gaagcccgcg gtcgcgacgg tcggggcgtc
     5221 gaagccaccg ctgacgtcga tcacgctgac cgagccctcc gggtcgatgg tgtagccttc
     5281 gttcggctcg ccctcgttgg ctaccagtac ggcattgccg tccggcgtga aggtcagcat
     5341 gtcaggcagc gcgccgacct cgacttcgct gatcttggcg agcgtgctcg tgtcgtagaa
     5401 cacgaccttg ccggggtctg tcttgacgtt cgcctcgatg gccacggcgg ccacgccgcc
     5461 atgcacggcg acgctgttcg cgccgccgcc ctccgagctg gcgtcgatgg tgtcgagcag
     5521 caccggattg gcagggtccg cgaggttcag cacgtcgatc gtgactgccg ccgcattgac
     5581 gacgaacagg cgctgcgtcg cggggtcgaa agcgacgatt tcggcggcgc tcgcgtcgaa
     5641 gatgcctgac gagtaacggc cgatgaggtt caggtcgagc agggcctcgc cggtgcccgc
     5701 aggcccgctt ggcccggccg gtccggtcgc gcccgccggt cccgctgcgc cgtcatcgcc
     5761 gtcacaaccc gcgagtccgg cgccgagggc cgccaccgcg gcaatggtca gaaatctcgt
     5821 caatctcatc atcaggctcc tgcatcatcg tggcaaagcc cggatgatgc ggagcccagg
     5881 tgacggcttg attacgagat ccttccggac cgttcgcggt agcgtgcgag ctcggccagg
     5941 gtgagccgga cgccccagta gagtgcaaac agcgccagga tgccgaacac ggcggcgcgc
     6001 aaatgcgcgg actggcccac ggcgacgaag gtgccgtaca gcatcacgca gccgaagccg
     6061 agggcgagga cgcagagccc gagaccctcc agcaattccc aggtaacgac tttcatgcgg
     6121 gccatggtgc cctaacgtgg tcatgccg
//
LOCUS       DAOMPG010000142         6128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5479_length_6128_cov_3.489050, whole genome shotgun sequence.
ACCESSION   DAOMPG010000142 DAOMPG010000000
VERSION     DAOMPG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_6128_cov_3.489050"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="PLS34_08440"
     CDS             <1..1524
                     /locus_tag="PLS34_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87533.1"
                     /translation="ERYEVHHGVEITDPAIVAAATLSHRYISDRQLPDKAIDLVDEAA
                     SRIRMEIDSKPEALDRLERRLIQLKIEREAMKKESDEASKKRLQALDGELQEVQRQYS
                     DLEEIWKAEKAEVQGASQIKEELERARVELETARRAGDLGRMSELQYGRIPELEKRLA
                     AAGEHEKKETRLLRNRVTDEEVAEVVSKWTGIPVARMLESERDKLIRMEEALEKRVVG
                     QGEALRAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALAEFMFDTEDAMV
                     RIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDTFN
                     VLLQVLDDGRLTDGHGRTVDFKNTVIVMTSNLGSHRIQELGSKPGSDTDYERMKNAVM
                     EVVAQHFRPEFINRVDDVVVFHALGREQIRIIVDIQLGHLRRRLAERDMRLELTDAAV
                     DRLAEAGFDPVYGARPLKRAIQQQVENPLAQRILRGEFGPGATVHVDAGPDGLAFAA"
     gene            1606..1968
                     /locus_tag="PLS34_08445"
     CDS             1606..1968
                     /locus_tag="PLS34_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRP87534.1"
                     /translation="MPIYEYLCGACGHVLDALQKHSDEPLVHCPHCGEPQLKRQLSAP
                     SFRLKGGGWYETDFKSDKRRNIAESDSTAGGGKAKESPDGCQARRGQDGQACRRQTGL
                     RQGRRRCLNTCAACWSRG"
     gene            1929..2582
                     /locus_tag="PLS34_08450"
     CDS             1929..2582
                     /locus_tag="PLS34_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRP87535.1"
                     /translation="MLKHLRRLLVTGLILWVPLLITFFVIRFLIRFVDKILLFLPPSW
                     RPEALLGFNIPGLGIILAVGILLATGLFAANFMGRRLVELGDRIVTRIPLVSGIYSGA
                     KQVAETLFGDSSTAFKRVLLVEYPRKGIWSMCFQTSEWVGEIQARTKEDVVCVFLPTT
                     PNPTSGFLLFVPKNELVPLDMPVDEGLRMIISLGVAVPRWKSPDAARASLAPPREQL"
     gene            2649..4412
                     /gene="aspS"
                     /locus_tag="PLS34_08455"
     CDS             2649..4412
                     /gene="aspS"
                     /locus_tag="PLS34_08455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRP87536.1"
                     /translation="MRSHYCGEIDEALIGQSATVVGWVHRRRDHGGVIFVDLRDREGL
                     LQVVFNPDDAATFALAESLRSEFVLQVAGEVRRRPEGTANPNLRTGQVELVARRLEVL
                     NRSETPPFHHDEPAGEDLRLRYRYLDLRRPEMLNNLRLRHRVTSALRRFLDGHGFVDV
                     ETPILTKATPEGARDYLVPARNFPGKFYALPQSPQLFKQLLMMSGLDRYYQIARCFRD
                     EDLRADRQPEFTQLDVETSFMDEAGITGLMETMVRELFRDVLGVELPDPFPRMSWHEA
                     VARFGTDRPDLRNPLELVEIADLVANVEFKVFAGPANDPQGRVTALRVPGGAGLSRKE
                     IDDYTAFVARYGARGLAYVKVNDAAGGRDGLQSPILKFLPDEAIAGILSRTGAATGDL
                     VFFGADKAKVVNDALGALRDRLGADLGLLEGNWRPLWVVDFPMFEWDEGERRWFALHH
                     PFTAPSVADADALRRDPGSALSRAYDMVLNGSEIGGGSIRIHDRAMQETVFELLGVRE
                     EEARLKFGFLLDALKFGCPPHGGIAFGIDRMVMLMAGAGSIREVIAFPKTQTASCPLT
                     DARARRPRAQAGVMGTLPNSA"
     gene            4413..4862
                     /gene="nudB"
                     /locus_tag="PLS34_08460"
     CDS             4413..4862
                     /gene="nudB"
                     /locus_tag="PLS34_08460"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HRP87537.1"
                     /translation="MGNVPNKRPESVLVVVYTRAGEVLLMERRQPPGWWQSVTGSLEA
                     DEMPWEAAVRELREETGLAADGLLDLGVNQRFTIAPAWRGRFAPGVTENLEHAFALRL
                     AAPVEIALDPAEHVRYEWLPLQAAAERATSHTNRAAIELIGRFIASC"
     gene            complement(4872..5945)
                     /locus_tag="PLS34_08465"
     CDS             complement(4872..5945)
                     /locus_tag="PLS34_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRP87538.1"
                     /translation="MTLLIVFFCLAIIVSFACSLWESVLLSITPSYVTRANSEGTSTG
                     RLLTEFKRDIDRPLSAILTLNTIAHTVGAIGVGAQAGRIWGSGGFQYMGMVLDYEVIV
                     AAVTTLAILVLSEIIPKTIGANNWERLAPFTAASIKALILVLWPLVWMSQTITRYLKK
                     DKGAPVFSRAEFLAMTEMGRAEGQLDEREAAVINNLLSLNRLRVYDVMTPRPVVIGGD
                     QTDTVEAFLAEHPDLPFSRLPVYEDVIDKVTGVVFKDDLLGALGTGDGHRTLAELERE
                     ALFVPDTLSLARALSQLLERRQQIAIVVDEYGVTAGVITVEDILETLLGLEIMDEKDS
                     VADLQRLARERWEARRGRLFDEG"
BASE COUNT          977 a         2095 c         2111 g          945 t
ORIGIN      
        1 gagcgctacg aggtgcatca cggcgtcgag atcaccgacc cggcgatcgt cgccgcggcc
       61 acgctgtcgc atcgctacat ctccgaccgc cagctgcccg acaaggccat cgacctggtg
      121 gacgaggccg cctcgcgcat ccgcatggag atcgactcca agccggaggc gctggaccgg
      181 ctggagcgcc gcctgatcca gctgaagatc gagcgcgagg ccatgaagaa ggagtcggac
      241 gaggcctcga agaaacgcct gcaggcgctg gacggcgagc tgcaggaagt gcagcgccag
      301 tactccgacc tcgaggaaat ctggaaggcc gagaaggccg aggtgcaggg cgcctcgcag
      361 atcaaggaag agctggagcg cgctcgtgtc gagctcgaga cggcgcgccg tgccggcgat
      421 ctcggccgca tgtccgagct gcagtacggc cgcatcccgg agctggagaa gcgcctggcc
      481 gcggccggcg agcacgagaa gaaggagacc cggctgctca ggaaccgcgt caccgacgaa
      541 gaggtggccg aggtcgtgtc caagtggacc ggcatcccgg tcgcacgcat gctggagagc
      601 gagcgtgaca agctgatccg catggaagag gcgctggaga agcgcgtcgt cggccagggc
      661 gaggcgctcc gggcggtgtc gaacgccatc cgccgctcgc gcgccgggct gtccgatccc
      721 aatcgcccga tcggctcctt cctgttcctc ggccccaccg gcgtcggcaa gaccgagttg
      781 tgcaaggcgc tggccgagtt catgttcgac actgaagacg ccatggtgcg catcgacatg
      841 tcggagttca tggagaagca ctccgtggcg cggctgatcg gcgccccgcc gggctacgtc
      901 ggttacgagg agggcggcta cctgaccgag gcggtacggc gccggccgta ctccgtgatc
      961 ctgctcgacg aagtcgagaa ggcgcatccg gacactttca acgtcctgct gcaggtgctc
     1021 gacgacggcc gcctgacaga cggccacggc cgcaccgtgg acttcaagaa caccgtcatc
     1081 gtcatgacct ccaacctcgg ctcgcaccgc atccaggaac tgggctcgaa accggggtca
     1141 gacaccgatt acgagcgcat gaagaacgcc gtgatggagg tggtggcgca gcatttccgg
     1201 ccggagttca tcaaccgcgt cgacgacgtg gtggtgttcc acgcgctggg gcgcgagcag
     1261 atccgcatca tcgtcgacat ccagctcggc cacctgcgcc gccggctggc ggagcgcgac
     1321 atgcgtctcg agctgaccga cgcggccgtg gaccggctgg ccgaggctgg tttcgacccg
     1381 gtctacggcg cgcggccgct gaagcgggcg atccagcagc aggtcgagaa cccgctggcg
     1441 cagcggatcc tgcgcggcga gttcgggccg ggcgccacgg tgcacgtcga cgccggcccc
     1501 gacggcctgg ccttcgccgc ctgacatata atgttgaatt tcggggcggc gtccccatgc
     1561 agcgtcgcaa cgggccgacc ggcccggcga tgagggtcca gcacgatgcc tatctatgaa
     1621 tacctgtgcg gcgcctgcgg ccacgtgctc gatgcgctgc agaagcactc ggacgagccg
     1681 ctcgtccact gcccgcactg cggtgagccg cagctcaagc gccagctgtc cgcgcccagc
     1741 ttccgcctga agggcggcgg ctggtacgag accgacttca agagcgacaa gcgtcgcaac
     1801 atcgccgaat ccgacagcac ggcgggcggc ggcaaggcga aggagtcgcc cgacggatgc
     1861 caagcccgcc gcggccaaga cggacaagcc tgccgccgcc aaaccggcct ccggcaaggc
     1921 cggcgacgtt gcttaaacac ctgcgccgcc tgctggtcac ggggctgatc ctctgggtcc
     1981 cgttgctgat cacgttcttc gtgatccgct tcctgatccg cttcgtcgac aagatcctgc
     2041 tgtttctgcc gccgagctgg cggcccgagg cgctgctcgg cttcaacatc cccggcctcg
     2101 gcatcatcct ggccgtgggc atcctgctcg cgaccggcct gttcgcggcg aacttcatgg
     2161 gccgccgcct ggtggagctg ggcgaccgca tcgtgacgcg catcccgctg gtcagcggca
     2221 tctattccgg cgccaagcag gtggccgaga cgctgttcgg cgacagcagc acggcgttca
     2281 agcgcgtgct gctggtcgag tacccgcgca agggcatctg gagcatgtgc ttccagacct
     2341 cggagtgggt cggcgagatc caggcgcgca ccaaggagga cgtggtgtgc gtgttcctgc
     2401 cgaccacgcc caacccgacc tccggcttcc tgctgttcgt gcccaagaac gagctcgtgc
     2461 cgctcgacat gccggtggac gaggggctgc gcatgatcat ttcgctcggc gtggcggtgc
     2521 cgcgctggaa gagccccgac gccgccaggg cgagccttgc gccgccccgg gagcagcttt
     2581 aacatccccg gcccttcgcc gccgttcgga tgacgggccg gcgccatccc ctgaaggaaa
     2641 tccaggtcat gcgtagtcat tattgcggtg agatcgacga ggcgctgatc gggcagtcgg
     2701 ccacggtcgt cggctgggtg catcgccgcc gcgaccacgg cggcgtgatc ttcgtcgacc
     2761 tgcgcgaccg cgagggcctg ctgcaggtgg tgttcaaccc cgacgatgcc gcgaccttcg
     2821 cgctcgccga gagcctgcgc tccgaattcg tgctgcaggt cgcgggcgaa gtgcgccgcc
     2881 ggccggaggg caccgccaac ccgaacctgc gcaccggcca ggtggagctg gtggcgcgcc
     2941 gcctcgaggt gctcaaccgc agcgagacgc cgcccttcca ccacgatgag ccggccggcg
     3001 aggacctgcg gctgcgctac cgctacctcg acctgcgccg cccggagatg ctcaacaacc
     3061 tgcgcctcag gcaccgcgtg accagcgcgc tgcgccgctt ccttgacggg cacggcttcg
     3121 tcgacgtcga gacgccgatc ctgaccaagg cgacgccgga gggcgcgcgc gactacctgg
     3181 tgccggcgcg caatttcccc ggcaagttct acgccctgcc gcagtcgccg cagctgttca
     3241 agcagctgtt gatgatgtcg ggcctggacc gctactacca gatcgcccgc tgcttccgcg
     3301 acgaggacct gcgcgccgat cgccagccgg agttcaccca gctcgacgtc gagacttcct
     3361 tcatggacga ggcgggcatc accgggctga tggagacgat ggtgcgcgag ctgttcaggg
     3421 acgtgctcgg cgttgaactg ccggatccct tcccgcgcat gagctggcac gaggccgtgg
     3481 cgcgtttcgg caccgaccgg cccgacctgc gcaacccgct ggagctggtc gagatcgccg
     3541 acctggtggc caacgtcgag ttcaaggtgt tcgccggccc ggccaacgac ccgcagggcc
     3601 gcgtcacggc gctgcgcgtg cctggtggcg ccggcctgtc gcgcaaggag atcgacgact
     3661 acaccgcctt cgtggcgcgt tacggcgcgc gcgggctggc ctacgtgaag gtcaacgatg
     3721 cggcgggcgg ccgcgacggc ctgcagtcgc cgatcctcaa gttcctgccc gacgaggcga
     3781 tcgccggcat cctgtcgcgc accggcgcgg ccaccggcga cctggtgttc ttcggcgccg
     3841 acaaggccaa ggtcgtcaac gatgcgctcg gcgcgttgcg cgaccggctg ggcgccgatc
     3901 tcggcctgct cgaggggaat tggaggccgc tgtgggtggt ggacttcccg atgttcgagt
     3961 gggacgaggg ggagcggcgc tggttcgcgc tacatcaccc ctttaccgcg ccgagcgtgg
     4021 ccgacgccga cgcgttgcgc cgcgatcccg gcagcgccct gtcgcgcgcc tacgacatgg
     4081 tgctgaacgg ctccgagatc ggcggcggct cgatccgcat tcacgaccgc gccatgcagg
     4141 aaaccgtgtt cgagctgctc ggcgtccgcg aggaggaggc gcggctcaag ttcggcttcc
     4201 tgctcgatgc gctgaagttc ggctgcccgc cgcacggcgg catcgccttc ggcatcgacc
     4261 gcatggtgat gctgatggcc ggcgccggca gcatccgcga agtgatcgcc ttcccgaaga
     4321 cccagaccgc cagctgcccg ctcaccgatg cgcgagctcg gcgtccgcgt gcgcaagccg
     4381 gagtaatggg gacactcccc aattccgcgt aaatggggaa tgtccccaat aaaaggccgg
     4441 agtcggtgct cgtggtggtg tacacgcggg ccggcgaagt gctgctcatg gagcgtcgcc
     4501 agccgcccgg ctggtggcag tcggtgaccg gcagcctgga agccgacgag atgccctggg
     4561 aggcggcagt gcgcgagctg cgcgaggaga ccgggctggc cgccgacggc ctgctcgact
     4621 tgggtgtcaa ccagcgcttc accatcgcgc cggcctggcg cgggcgcttt gcgccgggcg
     4681 tgaccgagaa cctggagcat gctttcgcat tgcggttggc cgcgccggtc gagatcgcgc
     4741 tcgatcccgc cgagcacgtc cgctacgagt ggctgccgct gcaggcggcg gcggaacggg
     4801 ccacctcgca cactaaccgg gccgccatcg agctcatcgg gcggttcatc gcgagctgct
     4861 gaaggctcgg ctcaaccctc gtcgaacagc cgcccgcggc gcgcctccca gcgctcgcgc
     4921 gccaggcgct gcaggtcggc caccgagtcc ttttcgtcca tgatctcgag acccagcaag
     4981 gtctcgagga tgtcctccac ggtgatcacg ccggcggtca cgccatactc gtcgaccacg
     5041 atggcgatct gctggcgccg ctcgagcagc tgggacaggg ccctggccag cgacagcgtg
     5101 tccggaacga acagcgcctc gcgctccagc tccgcgagcg tccgatggcc gtcgccggtg
     5161 cccagggcgc cgagcaggtc gtccttgaag accacgccgg tgaccttgtc gatgacgtcc
     5221 tcgtacaccg gcaggcggga gaacggcagg tccggatgct cggccaggaa ggcctccacg
     5281 gtgtcggtct ggtcgccgcc gatcaccacc gggcgcggcg tcatgacgtc gtagacccgc
     5341 aggcggttca ggctgagcag gttgttgatg acggcggcct cgcgctcgtc cagctggcct
     5401 tcggcccgcc ccatctcggt catggccagg aactcggcgc ggctgaacac cggcgcgccc
     5461 ttgtccttct tgaggtaacg ggtgatcgtc tggctcatcc acaccagcgg ccagagcacg
     5521 agaatcagcg ccttgatgga tgccgccgtg aacggcgcga gccgctccca gttgttggcg
     5581 ccgatggtct tcggaatgat ctccgagagc accaggatgg cgagggtcgt gaccgccgcc
     5641 acgatcacct cgtagtccag caccatcccc atgtactgga acccgccgct gccccagatc
     5701 cggccggcct gggcgcccac gccgatcgca cccaccgtgt gcgcgatggt gttgagcgtg
     5761 agaatggccg acagcggccg gtcgatgtcg cgcttgaact cggtcagcag gcggcccgtg
     5821 gaggtgccct cgctgttggc ccgggtgacg tagctgggcg tgatggacag cagcaccgat
     5881 tcccacaggg agcaggcgaa ggacacgatg atggcgaggc agaaaaacac tatcagcagg
     5941 gtcatgtttc cgttcgtctc ccaatgcgcg cggttcggct cgccttgaag tgattcagcg
     6001 gctctcgccg ttcgacagcg acttcagctc gtacaggatc tcgtgcgcct cgcgtgggct
     6061 gaggctgtcg gggtcgatgg ctgcgagtcg cgcctgcagg gcgccgggct cggcgaccgc
     6121 atcgaaca
//
LOCUS       DAOMPG010000143         5969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5626_length_5969_cov_3.241123, whole genome shotgun sequence.
ACCESSION   DAOMPG010000143 DAOMPG010000000
VERSION     DAOMPG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_5969_cov_3.241123"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLS34_08470"
     CDS             <1..272
                     /locus_tag="PLS34_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87539.1"
                     /translation="LALVTCVDFYAPDIMQRARRLLAPGGMLLVQVVLQPPGGDSPHR
                     AARNEALGFARGLQLHYYREGMIGGRALAQLLAQREPAAWPAFSD"
     gene            complement(262..447)
                     /gene="hemP"
                     /locus_tag="PLS34_08475"
     CDS             complement(262..447)
                     /gene="hemP"
                     /locus_tag="PLS34_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016536052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HRP87540.1"
                     /translation="MNKQTPQAPPPGGGVVNRLGRRPARIPSKVVLGGDNEVVIVHGS
                     EEYLLRVNPEGKLVLSR"
     gene            complement(542..1591)
                     /locus_tag="PLS34_08480"
     CDS             complement(542..1591)
                     /locus_tag="PLS34_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRP87541.1"
                     /translation="MTAAPPANPLVAAAWSLAPESIRPLGTGLINRTWLARAYDGELR
                     VLQQLNPMFPPEVMDDIAAVTAHLAARGLSTPRLVPDRDGRLWLPDPDGAWRVLTYMA
                     GQVFDAVASPAQAGEAGRLLASFHRALDDLEHEFRHVRPPVHEPARHLADLASAVESH
                     RSHRLYDAAAGLADDIFAAADALPALPRTPSRVVHGDPKISNLVFAADGERALCMLDL
                     DTLGRMALPYELGDALRSWCNVASEDDPRGSFSAELFAAAMGGYGAAAAGWIRPDEAG
                     AAVDATATIQVELAARFCADALNERYFGWNPALFPARGEHNLARARGQLNVHRSLLAQ
                     RHRLDEMAGAALARV"
     gene            1681..>2276
                     /locus_tag="PLS34_08485"
     CDS             1681..>2276
                     /locus_tag="PLS34_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRP87542.1"
                     /translation="MSNTVTAVHKFGGTSLAGAAEFRRVIGILEASGAQPLAVIVSAP
                     AGFTDALHALVHSAETGGDWPFGLESLISRCDGLAEALLSGAALAEWRAALARDRDDM
                     HDVLKATALIGRAPPEVDALIAGHGEIWSARLLSAALAARGLAADWFDARDALTVIPS
                     ELGPTVAWDQTRVRFAAAWTPPPAIRVVTGYIARDAHGG"
     assembly_gap    2277..2286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2287..4145
                     /gene="thrA"
                     /locus_tag="PLS34_08490"
     CDS             <2287..4145
                     /gene="thrA"
                     /locus_tag="PLS34_08490"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRP87543.1"
                     /translation="LGRNGSDFSASIFAALLDAEEVIIWTDVDGVMSGDPRRIPDVSV
                     IEELSWHEAMELAYFGAKVLHPRTMAPLVARSIPVWIRNTARPEGKGTRIGALSESGG
                     VKGVTTIAGMALLNLEGAGLIGVPGTASRLFEALRNAGISVVLISQGSSEHSICCAVP
                     EGDAARARDAVEKAFAAEIRQGQVEGVGMLPGCSILAVVGEGMRGMPGVAAKLFGALG
                     RAGVNVRAIAQGASERNISAVIPSDDADRALRAVHASFYLSPQAVSIGVIGPGGVGGQ
                     LLDQLAGEIPRLFKQSRVDLRVRAVAGSRRMVLAPRVVELGEWRDLLERGGATDLDAF
                     AAHVQAEHLPHAVIIDCSGVAEVAARYTDWLSRGIHVITPSKLAASGPLGTWRRLQQT
                     RRAAGTRYLGETTVGAGLPIIQTLRDLRQTGDEIIAIEGILSGTLAFLFNGFDGSKPF
                     SAIVREARALGFTEPDPRDDLSGRDVARKLVILAREMGLELELEQVDVESLVPPELAA
                     GSIDEFLDGLAAWDGGMRERLESARARDHVLRYVARLDGRSGRAAVGLEELPGDHPFA
                     YARLTDNVVQFVTRRYQDNPLVVQGPGAGPGVTAAGVFADLLRLTAYLGADV"
     gene            4142..5428
                     /gene="thrC"
                     /locus_tag="PLS34_08495"
     CDS             4142..5428
                     /gene="thrC"
                     /locus_tag="PLS34_08495"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRP87544.1"
                     /translation="MMRFTSTRGIASPVGAADAMLAGLAPDGGLYVPESFPQFGADCF
                     EGAAGLADTAAALLAPFFAGDPLAAELDAICREALDFPTPLVAAGDFQVLELFHGPTA
                     AFKDVGARFLAACLARLGGARERTVLVATSGDTGGAVAAAFAGRPGFRVVLLYPRGRV
                     SDRQEAQLTCWGGNVLSLRVDGDFDDCQRMVKEAFADAGLRARAGLTSANSISIGRLL
                     PQMSYYAWAALRTFRATGTPPGFIIPSGNLGNSLACVWARECGLPVGPVVLATNANET
                     VGDFFAGAPWSPRPTVQTLATAMDVGNPSNMERLLHLAGSAAAARSLARALRVSDEEI
                     RDAIRAGPQRYGQVWCPHTATGVRAWERLDEQARERPWVVVATAHPAKFEDVVEPLVG
                     AAVPLPPSLQELMARPRHFLEVGPSADELKELIAAY"
     gene            complement(5462..>5969)
                     /locus_tag="PLS34_08500"
     CDS             complement(5462..>5969)
                     /locus_tag="PLS34_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRP87545.1"
                     /translation="VLVADGGDFVGTASYVVRPRGPLRWLDPGAFGTLGVGGGFAIGA
                     ARARPSAQVWILYGDGSLGYSLIEFDSYVRHGLTPIAIVGNDACWAQIAREQVKMLKD
                     DVGVRLARTDYHRAAEGLGAAGLVITRDEDIAPTLAQARELAAAGRPVLVNVHLAETD
                     FREGSLSI"
BASE COUNT          784 a         2109 c         2223 g          843 t
ORIGIN      
        1 gcctggcgct ggttacctgc gtggacttct atgcccccga catcatgcag cgggcgcgcc
       61 ggttgctggc gcccggcggc atgctgctgg tgcaggtggt gttgcagccg cccggtggcg
      121 acagcccgca ccgcgccgcg cgcaacgagg cgctgggctt tgcccggggc ctgcagctgc
      181 attattaccg cgaaggcatg atcggcggcc gcgcgctggc gcagctgctc gcgcaacgcg
      241 agccggccgc gtggccggcc ttcagcgact gaggaccagc ttcccttccg ggttgacccg
      301 gagcaggtat tcctcgctcc cgtgcacgat gacgacctcg ttgtccccgc cgagcacgac
      361 cttgctcggt atgcgggccg ggcgcctgcc caggcggttc acgacgccgc cgccgggagg
      421 tggggcctgg ggggtctgct tgttcatcgg ccgtacctcg ggcgcgtggt gtcgagccca
      481 actttggggc tggcggggcg tcaacggcaa tcggggattc ccgcaggaga tggcgggcgg
      541 ttcagacccg cgccagcgcg gcgcccgcca tctcgtcaag ccggtggcgt tgcgccagca
      601 gcgagcgatg gacgttgagc tggcccctgg cgcgcgccag gttgtgttcg ccgcgagccg
      661 ggaacagggc gggattccag ccgaaatagc gctcgttgag cgcatccgcg cagaagcgtg
      721 ccgccagttc cacctggatg gtcgcggtcg cgtcgacggc ggccccagcc tcgtccgggc
      781 gaatccagcc cgcggccgcg gcgccgtagc cacccatggc ggcggcgaac agctcggccg
      841 agaaactgcc gcgcggatcg tcctccgagg ccacgttgca ccaggagcgc agcgcgtcgc
      901 cgagttcgta cggcagcgcc atgcgcccca gggtgtcgag gtccagcatg cacagggcgc
      961 gctcgccgtc cgccgcgaac accaggttgc tgatcttggg atcgccgtgc actacccggg
     1021 acggcgtgcg gggcagcgcc ggcagggcgt cggctgccgc gaagatgtcg tcggcgaggc
     1081 ctgcggcggc atcgtacagc cggtggctgc ggtgtgactc gaccgcgctg gcgaggtcgg
     1141 cgaggtgccg cgccggctcg tgcaccggcg ggcggacgtg gcggaactcg tgctcgaggt
     1201 cgtccagcgc gcggtggaag gacgccagca ggcggccggc ttcgcccgcc tgtgccgggc
     1261 tggcgacggc gtcgaacacc tgcccggcca tgtacgtgag cacccgccac gcgccgtcag
     1321 gatcggggag ccagagccgt ccgtcgcgat ccggcaccag gcgcggcgtc gacaggccgc
     1381 gcgccgcgag gtgcgccgtg accgccgcga tgtcgtccat cacctcgggc ggaaacatcg
     1441 ggttgagctg ttgcagcacc cgcagttcgc cgtcgtacgc gcgggccagc caggtgcggt
     1501 tgatcaggcc cgtgccgagc ggccggatcg actccggcgc cagcgaccag gctgcggcaa
     1561 cgagtggatt ggcggggggc gctgcagtca cgggtggcat cctccccccg ccgacgccac
     1621 gcggcaaccc ctggccggtg cgttgcagcg caacaccggc tgtcgtaggc tcgtcggact
     1681 atgagcaaca ccgtcacagc cgttcacaag ttcggcggca cgagcctggc cggtgcggcc
     1741 gagttccgcc gcgtcatcgg cattctcgag gcgagcggcg cgcagcccct ggccgtcatc
     1801 gtctcggcgc ccgccggctt cacggatgcg ctgcatgccc tggtgcactc ggccgagacc
     1861 ggtggcgact ggccgttcgg gctggaatcg ctcatcagcc ggtgcgacgg gctggccgag
     1921 gccctgctga gcggcgcagc gctggccgag tggcgtgcgg cgctggcgcg cgaccgggac
     1981 gacatgcacg acgtgctcaa ggccaccgcc ctgatcggcc gcgccccgcc ggaagtggac
     2041 gcgctcatcg ccgggcacgg cgaaatctgg tcggcgcgcc tgctgtccgc cgcgctcgcg
     2101 gcgcgcgggc tggcggccga ctggttcgat gcgcgcgacg cattgacggt gatccccagc
     2161 gagctgggcc cgaccgtcgc ctgggaccag acccgggtgc ggttcgcggc cgcatggacg
     2221 ccgccgccgg ccatccgcgt cgtcaccggc tacatcgcgc gcgacgccca cggcggnnnn
     2281 nnnnnncgct cggccgcaac ggcagcgact tctcggcgtc gatcttcgcc gcgctgctgg
     2341 acgcagagga agtcatcatc tggaccgacg tggacggcgt gatgagcggc gacccccgcc
     2401 gcatcccgga cgtctcggtg atcgaggaac tgtcgtggca tgaggccatg gaactggcgt
     2461 acttcggcgc caaggtgctc cacccccgca ccatggcgcc gctggtggcc cgcagcatac
     2521 cggtgtggat caggaacacc gcgcggccgg agggcaaggg cacgcgcatc ggcgcgctga
     2581 gcgaatccgg cggggtcaag ggcgtcacga ccatcgccgg gatggcgctg ctcaacctcg
     2641 agggtgccgg actcatcggg gtgcccggca ccgccagccg gctgttcgag gcgctgcgca
     2701 atgccggcat ctcggtagtg ttgatctcgc agggcagctc ggagcactcg atctgctgcg
     2761 cggtgccgga aggcgacgcg gcgcgggcgc gcgacgccgt ggagaaggcc ttcgcggccg
     2821 agatccgcca gggccaggtc gagggcgtcg gcatgctgcc gggctgcagc atactcgcgg
     2881 tcgtcggcga gggcatgcgc ggcatgcccg gcgtcgccgc caagctgttc ggcgcactcg
     2941 ggcgggccgg cgtgaacgtg cgggcgatcg cgcagggcgc ctcggagcgg aacatctccg
     3001 cagtcatccc cagcgacgat gccgatcgtg ccttgcgcgc cgtgcacgcc agcttctacc
     3061 tgtcgccgca ggcggtctcc atcggcgtca tcggcccggg cggtgtgggc gggcaactgc
     3121 tcgaccagct cgccggggaa attccgcgcc tgttcaagca gtcccgggtc gacctgcggg
     3181 tgcgggccgt ggcgggctca aggcgcatgg tgctggcgcc gcgggtggtg gagctgggcg
     3241 agtggcgcga cctgctcgag cggggcgggg ctaccgatct cgacgccttc gccgcgcatg
     3301 tccaggccga gcacctgccg catgcggtga tcatcgactg cagcggcgtc gccgaggtgg
     3361 cggcccgtta cacggactgg ctgtcgcgcg gcattcacgt catcacgccg agcaagctgg
     3421 ccgcgtcggg gccgctgggg acctggcggc gcctgcagca gacccggcgc gcggcgggca
     3481 cccgctacct cggcgagacc accgtgggtg ccggcctgcc gatcatccag acgctccgcg
     3541 acctgcgcca gacgggcgac gagatcatcg ccatcgaggg catcctgtcc ggcacgctgg
     3601 cgttcctgtt caacggcttc gacggctcga aaccgttctc ggccatcgtt cgcgaagcgc
     3661 gcgcgctggg cttcaccgag cccgatccgc gcgacgatct ctcgggccgc gacgtggcgc
     3721 gcaagctggt catcctggcg cgcgagatgg gcctggaact ggaactcgag caggtggacg
     3781 tcgagagcct cgtgccgccc gaactggccg ccggcagcat cgacgagttc ctcgacggcc
     3841 tcgccgcctg ggacggcggg atgcgggaac ggctggagtc ggcgcgcgcc cgcgaccatg
     3901 tgctgcgcta cgtggcgcgg ctcgacgggc gcagcggccg cgccgcggtg ggcctcgagg
     3961 aactgcccgg cgatcatccc ttcgcctatg cacgactgac cgacaacgtg gtgcagttcg
     4021 tcacccggcg ctaccaggac aacccgctgg tcgtgcaggg ccccggcgcc gggccgggcg
     4081 tgaccgcggc gggcgtgttc gccgacctgc tgcgcctgac ggcgtacctg ggcgcggacg
     4141 tctgatgcga ttcaccagca cgcgcggcat cgcgtcgccg gtgggcgccg cggacgcgat
     4201 gctcgcggga ctggcgccgg acggcgggct gtacgtgccg gagtcctttc cccagttcgg
     4261 tgcagactgt ttcgagggcg ccgcgggact ggccgacacc gccgccgcgc tgctggcgcc
     4321 atttttcgcc ggcgacccgc tggccgcaga actggacgcg atctgccgcg aggcgctgga
     4381 cttcccgacg ccgctcgtgg ctgccggcga cttccaggtg ctggagttgt tccacggtcc
     4441 gaccgccgcg ttcaaggacg tcggggcacg cttcctcgcg gcgtgcctgg cacggctcgg
     4501 cggcgcacgc gagcgcacgg tgctggtggc cacctcgggc gacacgggcg gcgcggtcgc
     4561 ggcggccttc gccggccggc cgggattccg cgtggtcctg ctgtacccgc ggggccgggt
     4621 gtccgaccgc caggaggccc agctgacctg ctggggcggc aacgtgctga gcctgcgcgt
     4681 ggatggcgac ttcgacgact gccagcgcat ggtcaaggag gcgtttgcgg acgccgggct
     4741 gcgcgcacgc gccgggctga cctcggccaa cagcatcagc atcggccgcc tgctgccgca
     4801 gatgtcctac tacgcctggg cggcgctgcg caccttccgc gcgaccggta caccgccggg
     4861 tttcatcatt cccagcggca acctgggcaa ttcgctggcc tgtgtctggg cgcgtgagtg
     4921 cggcctgccg gtggggccgg tcgtgctggc gaccaacgcc aacgagaccg tcggggactt
     4981 tttcgccggc gcgccctggt cgccgcggcc caccgtgcag acgctggcga cggccatgga
     5041 cgtcggcaac ccgagcaaca tggagcggct gctgcacctg gcgggctccg cggccgcggc
     5101 ccgcagcctg gcgcgcgcgc tgcgcgtctc ggacgaggaa atccgcgacg ccatccgggc
     5161 cgggccgcag cgctacggcc aggtctggtg tccgcacaca gcgacgggcg tgcgcgcctg
     5221 ggagcggctg gacgaacagg cgcgggagcg cccctgggtg gtcgtcgcga ccgcacatcc
     5281 cgccaagttc gaggacgtcg tcgaaccgct ggtcggtgcg gcggtgccgc tgccaccgtc
     5341 gctgcaggaa ctgatggcac ggccgcggca cttcctcgag gtcggcccgt cagccgacga
     5401 actcaaggag ctcatcgcgg catattgact gcgcccgttg gccggccgcg gcccggcggg
     5461 gctagatcga cagcgagccc tcgcggaaat cggtctccgc caggtgaacg ttgaccagca
     5521 cggggcggcc ggccgcagcg agttccctgg cctgcgccag ggtcggcgcg atgtcctcgt
     5581 cgcgggtgat gaccaggccc gccgctccga ggccctcggc agcgcggtga tagtccgtgc
     5641 gcgccagtcg cacgccgacg tcgtccttga gcatcttgac ctgctcgcgc gcgatctgcg
     5701 cccagcaggc gtcgttgccg acaatggcga tcggcgtcag gccgtggcgc acgtagctgt
     5761 cgaactcgat caggctgtag ccgagcgagc cgtcgccgta gaggatccag acctgcgccg
     5821 agggccgcgc gcgcgccgcg ccgatggcga aaccgccgcc cacgccgagc gtgccgaagg
     5881 cgccggggtc gagccagcgc agcggcccgc gcggtcgcac gacatacgaa gcagtgccga
     5941 caaagtcacc gccgtccgcc accagcacg
//
LOCUS       DAOMPG010000144         5891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5718_length_5891_cov_3.589102, whole genome shotgun sequence.
ACCESSION   DAOMPG010000144 DAOMPG010000000
VERSION     DAOMPG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_5891_cov_3.589102"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLS34_08505"
     CDS             <1..360
                     /locus_tag="PLS34_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRP87546.1"
                     /translation="RRKRQENQMAVKKAAAAVVKKAKPPTKAEIVTQIANKTGVAKKD
                     VTAAFDELNAIIKKSLKSANEFSMPGLMKIKVVKKPATKARLGKNPFTGEEIMIKAKP
                     ARKTVKVTALKGLKDMV"
     gene            complement(405..872)
                     /locus_tag="PLS34_08510"
     CDS             complement(405..872)
                     /locus_tag="PLS34_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRP87547.1"
                     /translation="MIGKLKHLLRDALGEARPEAGGGHDLRLATAALLLEMARADREE
                     TPAERQAAEAMLARHFSLERAAAEALLARAEGSLHEAVSLYEFTRVLNDTLTAAERRK
                     VVEMVAEVAATDGHFDKHEQHLLSKLADLLHLRRAEYASIRASVLQRGPDRPE"
     gene            complement(869..1537)
                     /locus_tag="PLS34_08515"
     CDS             complement(869..1537)
                     /locus_tag="PLS34_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP87548.1"
                     /translation="MKRLYRDTPWRPPRPEMLEIQGPAGPLEAIVEDPDGEPPALAVV
                     CHPHPLYQGTMRNKVVHTVARAANRLGAPAVRFNFRGVGASAGAWDEGRGETEDALAV
                     IEWARRRWPQRPLWLAGFSFGAFVALRAAAAAVPAALVTVAPPVSRFPVGATPDPGCP
                     WLVVQGEADELVDYREVVAWAEARPAARLALLPETSHFFHGRLAELQQVVQEFLHEAQ
                     EPPS"
     gene            1573..2787
                     /gene="rocD"
                     /locus_tag="PLS34_08520"
     CDS             1573..2787
                     /gene="rocD"
                     /locus_tag="PLS34_08520"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRP87549.1"
                     /translation="MHRIIELEDRYCAHNYHPLPVVLVRGEGPYVWDDQGKRYVDMMS
                     AYSAVSHGHAHPRIITALVEQARRLNIVSRAFHTDRLAAFLERACTLTGQDMALPMNT
                     GAEAVETALKAARKWAWQVKGVPDGAAEIIACEGNFHGRTIAVCGLSTERQYREGFGP
                     FPPGLRTVPYGDAAALEAAITPHTAAFLVEPIQGEGGIVIPPQGYLAACAEICRRHDV
                     MLIADEIQTGLGRTGKLLACEHEGVKPDGLVLGKALGGGVLPVSMFLARRELMGVFRP
                     GDHGSTFGGNPLAAAVGLEALRILEDEDLAAQSAAKGAWLLGELKKIASPLVREVRGR
                     GLFIGVEIDPAKARARTVVERLMDRGLLSKETHETVVRLAPPLAIPQELLEWSLEQIR
                     EVFAELAAPAHA"
     gene            2810..3442
                     /locus_tag="PLS34_08525"
     CDS             2810..3442
                     /locus_tag="PLS34_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRP87550.1"
                     /translation="MKLIVEGIQHGKPIPARFAFGVPDAATHVRLGENLNPAIRWENL
                     PEGTRTLVLICVDTDVPTRPDDVNQEGRTVPADLPRGRFYHWVVVDLAPDSMGIAEGA
                     CSRGVTPRGKQSPEGPEGSRQGRNDYTAWFEGDKDMGGVYLGYDGPCPPWNDSIPHHY
                     HFVLYATDLERCPVTGAFSAEEVETALTGHVLARAEVTGLYSLNPDVPAA"
     gene            complement(3464..3937)
                     /locus_tag="PLS34_08530"
     CDS             complement(3464..3937)
                     /locus_tag="PLS34_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRP87551.1"
                     /translation="MNRPLRFLLSAAIASAVVLALMVSALWAFGLFDRAEEVIYAHPV
                     EVLSQSDRVDVAELVGAPQRPLPARPVDRPVEPLVVPRREVSGFVQIEVEVDPDGRVQ
                     RAEVVNALPAGVYEEQALRQVRQRRYPPRDGGGSYVEVVPFKIAADELPPGAPGD"
     gene            complement(3956..4402)
                     /locus_tag="PLS34_08535"
     CDS             complement(3956..4402)
                     /locus_tag="PLS34_08535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRP87552.1"
                     /translation="MRRLAMLIATLSAPTMHGCAPPAEGPAETPAAAPTELVITELAA
                     GEGAAIEPGSTAVMHYTGWLFDAAAPDGKGAKFDSSRDRGQPFRFPLGAGRVIQGWDR
                     GVPGMKVGEQRRLVIPPELGYGSRGAGGVIPPNATLVFDVELLGIE"
     gene            complement(4483..4707)
                     /locus_tag="PLS34_08540"
     CDS             complement(4483..4707)
                     /locus_tag="PLS34_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87553.1"
                     /translation="MINKFLHGRELPAEILDDERSWQGDHDPYLGMLAHEGRKHREGP
                     PPRGPRLAGPADDGDSYLADLVHARPADSD"
     gene            complement(4712..4876)
                     /locus_tag="PLS34_08545"
     CDS             complement(4712..4876)
                     /locus_tag="PLS34_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87554.1"
                     /translation="MEVMLQWADDLDDLLAAGLQLVEAYGYRLLGLAGVAAAGISGIA
                     IGIGVGVLWG"
     gene            complement(4971..5792)
                     /locus_tag="PLS34_08550"
     CDS             complement(4971..5792)
                     /locus_tag="PLS34_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87555.1"
                     /translation="MPLIVLLSLLPLLAGIGWLARARRLRRERRDRVREVLLQANVGG
                     DCFIADSVDALPDPAARYLLHALAPDAPLARSVDVRLSGSLRVGADDWVPFEARQRIC
                     AERGFIWEARINVVRRLGIEGADWLLGDEAGIEYALEDWWPVVGRQGPETSRSALGRL
                     MVELIWLPSALTPQRGASWTRGDADRAVVTPAGGKAPMTVAVGDDGRLREASVMRRRV
                     SANGETSIASYGVSVEAEERWGELVVPARLTAAWGIGTDDRQDFMRLVVEDLRWL"
BASE COUNT          944 a         2168 c         2013 g          766 t
ORIGIN      
        1 agacgaaaaa ggcaggagaa ccagatggca gttaagaagg cagccgccgc cgtagtgaaa
       61 aaagccaagc cgccgaccaa ggcagaaatc gtcacgcaga tcgcgaacaa gaccggcgtg
      121 gccaagaagg atgtcacggc cgcgttcgac gaactgaacg cgatcatcaa gaagtcgttg
      181 aagtccgcca acgagttctc gatgcccggc ctgatgaaga tcaaggtcgt gaagaagccc
      241 gccaccaagg cgcgcctcgg caagaacccg ttcaccggcg aagagatcat gatcaaggcc
      301 aagccggcgc ggaagaccgt caaggtcacc gccctcaagg gcctcaagga catggtctga
      361 ggccaggctg acgacgcggg cccggcggac tgcgccgggc cccgttactc cgggcgatcc
      421 ggcccgcgct gcagcacgga cgcccggatg cttgcgtact ccgcgcggcg caggtgcaac
      481 aggtcggcga gcttcgacag caggtgctgc tcgtgcttgt cgaaatggcc gtcggtggcc
      541 gccacttccg ccaccatctc gacgaccttg cggcgctccg ccgcggtcag cgtgtcgttc
      601 aggacccgcg tgaactcgta gagcgacacg gcctcgtgca ggctgccctc ggcgcgcgcc
      661 agcagcgcct cggccgccgc ccgctcgagc gaaaagtgcc gcgccagcat ggcctcggcc
      721 gcctgtcgct ccgccggcgt ttcctcgcga tccgcgcgcg ccatttccag cagcagcgcc
      781 gccgtggcca gccgcaggtc gtgacctccg cccgcctcgg ggcgcgcttc gcccagggcg
      841 tcgcgcaaca ggtgcttgag cttgccgatc aactgggcgg ctcctgtgcc tcgtgaagaa
      901 actcctgcac cacctgctgc agctccgcca ggcggccgtg aaagaaatgg ctggtctcgg
      961 gcagcaacgc caggcgcgcc gccggccgcg cctcggccca ggcaacgacc tcgcgatagt
     1021 cgaccagttc gtccgcctcg ccctgcacca ccagccacgg acagcccggg tcgggagtcg
     1081 cgccgaccgg gaaacggctc accggcggcg cgaccgtgac cagcgccgcg ggcacggcgg
     1141 ccgccgcggc gcgcaaggcc acgaacgccc cgaaggaaaa gccggccagc cacagggggc
     1201 gttgcggcca gcgccgccgc gcccattcga tcaccgccag cgcgtcctcc gtttcaccgc
     1261 gcccctcgtc ccaggcgccg gcgctcgcac cgacgccacg aaagttgaaa cgcaccgccg
     1321 gcgcacccag gcggttggcc gcccgggcca cggtatgtac caccttgttg cgcatcgtcc
     1381 cctggtagag cgggtgtgga tgacagacca cggccagcgc cggcggttcc ccgtccgggt
     1441 cctccacgat cgcctcgagc ggccctgccg ggccctggat ctccagcatt tccggccgcg
     1501 gcggccgcca cggcgtatca cggtacaatc gcttcatctg catagaatgc cgcaagccgg
     1561 ggaacgcgcg ccatgcaccg catcatcgag ctcgaggatc gctactgcgc ccacaactac
     1621 caccccttgc cggtggtgct ggtgcgcggc gagggcccgt acgtctggga cgaccagggc
     1681 aagcgttacg tggacatgat gagcgcctac tccgcggtga gccacggcca cgcgcacccg
     1741 cgcatcatca ccgcactcgt cgagcaggcg cggcggctga acatcgtctc gcgcgccttc
     1801 cacaccgacc ggctggccgc cttcctggag cgagcctgca ccctcaccgg ccaggacatg
     1861 gcgctgccga tgaacaccgg cgccgaagcc gtcgagacgg cgctcaaggc ggcgcgcaaa
     1921 tgggcgtggc aggtcaaggg cgtgcccgac ggcgcggcgg aaatcatcgc ctgcgaaggc
     1981 aatttccacg gccgcaccat cgccgtctgc gggctgtcca ccgagcgcca gtaccgcgag
     2041 ggcttcggcc ccttcccgcc cggcctgcgc accgtgccct acggggacgc cgccgcgctg
     2101 gaggccgcca tcacgccaca caccgcggct ttcctggtcg agcccatcca gggcgaaggc
     2161 ggcatcgtca tcccgccaca gggctatctc gccgcctgtg cggagatctg ccggcgccac
     2221 gacgtgatgc tgattgccga cgagatccag accgggctcg gccgcaccgg caagctgctg
     2281 gcctgcgagc acgagggcgt caagcccgac gggctggtgc tcggcaaggc gctcggcggc
     2341 ggcgtgctgc cggtgtcgat gtttctcgca cgccgcgagc tgatgggcgt gttccggccc
     2401 ggcgatcacg gcagcacctt cggcggcaac ccgctggccg cggccgtcgg actggaggca
     2461 ctgcgcatcc tcgaggacga ggacctggcg gcacagagcg cggccaaggg cgcctggctg
     2521 ctcggcgagc tgaagaagat cgccagcccg ctggtgcgcg aggtgcgcgg ccgcgggctg
     2581 ttcatcggcg tggagatcga cccggcgaag gcgcgcgccc gcacggtggt cgagcgcctc
     2641 atggaccgcg gcctgctcag caaggaaacc cacgagaccg tcgtgcgcct cgcgccgccg
     2701 ctcgccatcc cgcaggagtt gctcgagtgg tcgctcgagc agatccgcga ggtgttcgcc
     2761 gagctggccg cgccggcgca cgcctgacca agcacaggag gcagacagca tgaaactgat
     2821 cgtcgaaggg atccagcacg gcaagccgat acccgccagg ttcgcgttcg gtgtgcccga
     2881 cgcggccacg cacgtgcgcc tgggcgagaa cctcaacccg gccattcgct gggagaacct
     2941 gccggaaggc acgcgtacgc tggtgctgat ctgcgtggat accgacgtgc ccacgcgacc
     3001 cgacgacgtc aaccaggaag gccgcaccgt gccggccgac ctgccgcgcg ggcgcttcta
     3061 ccactgggtg gtcgtcgacc tggcgccgga ctcgatgggc atcgccgagg gcgcctgcag
     3121 tcgcggcgtg acgccgcgcg gcaagcagtc accggaaggg ccggagggct cgcgccaggg
     3181 gcgcaacgac tacaccgcct ggttcgaggg cgacaaggac atgggtggcg tgtacctggg
     3241 ttacgacggc ccgtgcccgc cgtggaacga ttccatcccg caccactatc acttcgtgct
     3301 ctacgccacc gacctggagc gctgtccggt gacgggcgcg ttctcggcgg aagaagtgga
     3361 gactgcgctg accggccacg tgctggcgcg cgcggaggtg accggcttgt actcgctcaa
     3421 cccggacgtg ccggcggcct gacccggcgc ggacgaaccg gggctagtcg ccaggtgccc
     3481 cgggcggcaa ctcgtccgcg gcgatcttga agggcacgac ctcgacatag ctgccgccgc
     3541 cgtcgcgcgg cgggtagcgg cgctggcgca cctggcgcag cgcctgctcc tcgtagacgc
     3601 cggccggcag cgcattgacc acctcggcac gctgcacgcg gccgtccgga tcgacctcga
     3661 cctcgatctg cacgaagccg ctcacctcgc ggcgcggcac caccagcggc tcgaccggcc
     3721 ggtccaccgg gcgtgccggc agcggccgtt gcggggcgcc caccagctcg gcgacgtcga
     3781 cgcgatcgct ctgcgagagg acctcgaccg ggtgggcgta gatgacctcc tcggcgcggt
     3841 cgaacaggcc gaaggcccac agcgccgaca ccatcagcgc cagcacgacc gccgaggcga
     3901 tcgccgcgga aagcaggaag cgcagcggcc ggttcaccgg atcttcagcc ctcgtctact
     3961 cgatgcccaa cagttcgaca tcgaaaacga gcgttgcgtt cggcggaatc acgccgccgg
     4021 cgccgcgact gccgtagccc agctccggcg ggatcaccag ccggcgctgc tcgccgacct
     4081 tcatgccggg cacgccccgg tcccagccct ggatgacacg accggcgccc agcgggaaac
     4141 gaaaaggctg gccgcgatcg cgcgagctgt cgaacttggc gcccttgccg tccggtgccg
     4201 cggcatcgaa cagccagccg gtgtaatgca tcacagccgt gctgccgggc tcgatggccg
     4261 cgccctcgcc ggcggcgagc tcggtgatca ccagctcggt cggggccgcc gcgggagttt
     4321 ccgcgggacc ttcggcgggc ggcgcgcaac cgtgcatggt gggggcggac agcgtggcga
     4381 tcagcatggc gagacggcgc atggcgtggc tccaggaaaa cggagcccgc accatagagc
     4441 agtcgccgcc gggcgcgcaa gccgcgcgct gcaacgctcc cgctagtcgc tgtccgccgg
     4501 tcgcgcgtgg accaggtcgg cgagatagga atcgccgtcg tcggccggcc cggcgaggcg
     4561 cggcccgcgc ggcggcgggc cttcgcggtg cttgcggccc tcgtgcgcga gcatgccgag
     4621 gtagggatcg tggtcgccct gccagctgcg ctcgtcgtcg aggatctcgg caggcagttc
     4681 gcggccatgc aggaacttgt tgatcatgat ttcagcccca cagcacgccc acgccgatgc
     4741 cgatcgcaat gcccgagatg cccgccgcgg cgacgccggc cagccccagc aggcggtagc
     4801 cgtaggcctc gaccagctgc agccccgcgg ccagcaggtc gtcgaggtcg tccgcccatt
     4861 gcagcatcac ttccaagcgc cggttcccga gatacgattg cgttgtgtgc aagctgccac
     4921 ggcgccgctg cggataccgt gacaggattc gcgaaaatgc tgcgccaggc tcacagccag
     4981 cgcaggtctt cgacgacgag ccgcatgaag tcctggcgat cgtcggtgcc gatgccccac
     5041 gccgccgtga gccgcgccgg cacgaccagt tcgccccagc gctcctccgc ttccacgctg
     5101 acgccgtagg acgcgatcga ggtctcgccg ttggcggaca cccggcgacg catcacgctg
     5161 gcctcgcgca gccggccgtc gtcgcccacc gcaacggtca tcggcgcctt gccgccggcc
     5221 ggcgtcacca ccgcgcggtc ggcgtcgccg cgcgtccagc tcgcgccgcg ctgcggcgtg
     5281 agcgccgaag gcagccagat cagttccacc atgagccggc cgagcgccga ccgcgacgtt
     5341 tccggaccct gccgcccgac caccggccac cagtcctcga gggcatactc gatgcccgcc
     5401 tcgtcgccca gcagccagtc cgcgccctcg atgccgagcc gccgcacgac gttgatgcgc
     5461 gcttcccaga tgaaaccgcg ttccgcacag atgcgctgcc gcgcctcgaa cggcacccag
     5521 tcgtccgccc ccactcgcag gctgccgctc agccgcacgt cgacggacct tgccagcggt
     5581 gcgtcgggcg ccagcgcatg cagcaggtag cgggcggcgg ggtcgggcag ggcgtcgacc
     5641 gagtcggcga tgaagcagtc gccgccgacg ttggcctgca gcaacacttc gcgtacccgg
     5701 tcacggcgct cgcggcgcag tcggcgcgcg cgggccagcc agccgattcc ggccagcagc
     5761 ggcaacagcg acagcaggac gatcagtggc atcggcgggc aaccctcgtg acggcgcgcg
     5821 ccgggacgat cacgtcagtc ggcgggtaaa tcccggtcgg gaggcgcagg cgcgcggtcc
     5881 gcggagtttc c
//
LOCUS       DAOMPG010000145         5877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5740_length_5877_cov_4.761937, whole genome shotgun sequence.
ACCESSION   DAOMPG010000145 DAOMPG010000000
VERSION     DAOMPG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5740_length_5877_cov_4.761937"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /gene="xseA"
                     /locus_tag="PLS34_08555"
     CDS             complement(<1..711)
                     /gene="xseA"
                     /locus_tag="PLS34_08555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRP87556.1"
                     /translation="MQTHDFGLPERDVWTISRLNREVRLLIESGLPALWIEGEVSNLA
                     RPASGHVYFSLKDEGAQVRCALWRSAALKLSFTPRNGMQLLVRARVSVYEPRGEYQLI
                     VEYAEESGEGALRRRFEELKARLRAEGLFDEAAKRPLPRVPRRIGVITSPTGAAVRDI
                     LHVLKRRCPMVPVVIYPVPVQGEGAADKIAAALALASRRAECDVLILARGGGSLEDLW
                     SFNEEVVAHAIRAAAIPLI"
     gene            801..2267
                     /gene="guaB"
                     /locus_tag="PLS34_08560"
     CDS             801..2267
                     /gene="guaB"
                     /locus_tag="PLS34_08560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRP87557.1"
                     /translation="MRLIQEALTFDDVLLEPAYSEVLPRDVSLRTRLTKEIQLQLPIV
                     SAAMDTVTEARLAIALAQEGGIGVIHKNMTGEAQAREVRRVKKYESGIINDPITVAPG
                     KTIREVLDLTRAKGISGVPVVDGDRTVGIVTNRDLRFETRLDLPVSEVMTPRDRLVTV
                     REGAAKDEVLRLLHQHRIEKVLIVDDDFRLKGMITAKDFQKATDFPRACKDERGALRV
                     AAAVGTSADTDERVEALVAAGVDVIVVDTSHGHTLGVLQRVRRVKDAFPHIQVIGGNI
                     VTGDAARALVEAGADGVKVGIGPGSICTTRIVAGVGVPQVSAVANVAAALEGTGVPLI
                     ADGGIRYSGDLAKAIVAGAHSIMVGGLFAGTEESPGEVELYQGRSYKAYRGMGSLGAM
                     SQRHGSSDRYFQDATDEVEKLVPEGIEGRVPYKGSLLGIIHQLAGGLRAAMGYTGCST
                     IEEMRSKPKFVRITNAGVRESHVHDVAITKEAPNYRVD"
     gene            2281..3858
                     /gene="guaA"
                     /locus_tag="PLS34_08565"
     CDS             2281..3858
                     /gene="guaA"
                     /locus_tag="PLS34_08565"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRP87558.1"
                     /translation="MTTDIHAQRILILDFGSQYTQLIARRVREAGVYSEIRAWDCPAE
                     DIRHFAPAGIILSGGPESVTVGEGPRAAAAVFELGVPVLGICYGMQTMAAQLGGEVAN
                     SAHREFGYAQVRITGSSSLLDDIEDHEDAAGSALLDVWMSHGDRVETLPPGFIGIAGT
                     DSAPLAGIADDGRRFYGLQFHPEVTHTRQGARIVSRFVHDICGCGSDWNAGNIVEDSI
                     ARVRAQVGGGKVLLGLSGGVDSSVVAALLHRAIGEQLTCVFVDHGLLRLGEGDQVMAT
                     FARHMGIRVIRVDAEERFLAALAGVTDPEEKRKIIGRLFIEVFEEQAQQLEDVDWLAQ
                     GTIYPDVIESAGASTGKAHVIKSHHNVGGLPERMRMKLVEPLRELFKDEVRKIGLELG
                     LPWDMVYRHPFPGPGLGVRILGEVRKEFAELLRRADDIFITELRRHELYDQVSQAFAV
                     FLPVRSVGVMGDGRRYDYVVALRAVETIDFMTARWAHLPYEFLDLVGRRIINEVAGIS
                     RVTYDISGKPPATIEWE"
     gene            3913..5466
                     /locus_tag="PLS34_08570"
     CDS             3913..5466
                     /locus_tag="PLS34_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010452121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRP87559.1"
                     /translation="MAGPVIAADKPQKVTLEKGKEYYWCRCGRSQSQPFCDGSHKSTD
                     ITPKKFVAEKDGDAYLCACKHTGNQPFCDGTHKRFSAEQVGKEGPGPQAIHSGDGGRP
                     KAEATKEEPTVAFIHELARDGLKSHGAHGPMTAMGVPRHELPHWDDLQILVAQLADKP
                     LQEDVAVGTELVIGPEARKPLVLKIPLFVSDMSFGSLSEEAKIALARGAELAGTGICS
                     GEGGMLPEEQQANSRYFYELASAMFGFREELLARVQAFHFKGGQGAKTGTGGHLPASK
                     NVGRIAEVRGLTAGEPAVSPPTFRDLSSKKDFRKFAARVRDITGGVPVGFKLSANHIE
                     RDVQFALDAGADYIILDGRGGATGSAPQIFRDHISVPTIPALARARRHLDKQKAGGRV
                     TLIITGGLRMPMDFVKAMALGADGVAIANAAIQAVGCVAARMCHTNNCPSGVATQKPE
                     LRKRIDVDRSARQLCNFLEASVQLMQVMARACGHDHLSQFNPGDLSTWKRDMALLSGV
                     KFGGVLPPR"
     gene            5463..>5877
                     /gene="tadA"
                     /locus_tag="PLS34_08575"
     CDS             5463..>5877
                     /gene="tadA"
                     /locus_tag="PLS34_08575"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRP87560.1"
                     /translation="MSVAGLSGTVDELYMRRALALAACAEAEGEVPVGAVLVAGGEVI
                     AETWNRCITLSDPAGHAEVLALRAGGQALGNYRLPGATLYVTLEPCAMCAGAMVHARI
                     ARLVYGAADPKTGAAGSVFDLVQAPALNHRIEVTAG"
BASE COUNT          955 a         2025 c         2046 g          851 t
ORIGIN      
        1 gatcagcggg atcgccgcgg cgcggatggc atgggccacg acctcttcgt tgaaactcca
       61 caggtcctcc agcgagccgc cgccgcgggc gaggatcagg acgtcgcact cggcgcgccg
      121 gctcgccagc gccagggcgg cggcgatctt gtcggcggca ccctcgccct ggaccggcac
      181 cgggtagatc accaccggca ccatcgggca gcgccgcttg agaacgtgca ggatgtcgcg
      241 caccgccgcg ccggtgggcg aggtgatcac gccgatccgg cgcggcacgc gcggcagcgg
      301 ccgcttggcg gcttcgtcga acagcccctc ggcgcgcagc ctcgccttga gctcctcgaa
      361 cctgcgccgc agcgcgccct cgccggattc ctccgcgtac tcgacgatca gctggtattc
      421 gccgcgcggc tcgtagacgc tcacgcgggc gcgcaccagc agctgcatgc cgttgcgcgg
      481 cgtgaaggag agtttcagcg ccgccgagcg ccacagcgcg cagcgcacct gtgcaccctc
      541 gtccttgagc gagaagtaca cgtggccgga ggccggccgt gccaggttgg agacctcgcc
      601 ttcgatccac aaggccggca gaccggactc gatcagcagc cgcacctcgc gattgaggcg
      661 cgatatggtc cagacgtcgc gttccggcag gccgaagtcg tgcgtttgca tgcaccgatc
      721 ataccgcgag atttaccccc gccggggcag cgcgtagaat ggccggttta ccgctaccca
      781 ctgcccccga gggaccagcg atgcgtttga tccaggaagc cctcactttc gacgatgtcc
      841 tgctcgagcc cgcctactcg gaggtgctgc cgcgcgacgt cagcctgcgc acccgcctga
      901 cgaaagagat ccagctgcag ctgccgatcg tctcggccgc catggacacg gtcaccgagg
      961 cgcgcctggc catcgcgctg gcccaggaag gcggcatcgg cgtgattcac aagaacatga
     1021 ccggcgaggc gcaggcccgc gaggtgcgcc gcgtcaagaa atacgaaagc ggcatcatca
     1081 acgatcccat cacggtggcc ccgggcaaga ccatccgcga agtgctcgac ctgacccgtg
     1141 ccaagggcat ctccggcgtg ccggtggtgg acggcgaccg cacggtcggc atcgtcacca
     1201 atcgcgacct gcgtttcgag acccgcctgg acctgcccgt gtccgaggtg atgacgccgc
     1261 gcgaccggct ggtgacggtg cgcgagggcg ccgccaagga cgaggtgctg cggctgctgc
     1321 accagcatcg catcgagaaa gtgctgatcg tcgacgacga tttccgcctc aagggcatga
     1381 tcaccgcgaa ggacttccag aaggcgaccg attttccgcg cgcctgcaag gacgaacgtg
     1441 gcgcgctgcg ggtggcggcg gcggtcggca ccagcgccga caccgatgag cgcgtcgaag
     1501 cgctggtggc cgccggcgtg gacgtcatcg tggtggacac ttcgcacggc catacgctgg
     1561 gcgtgctgca gcgcgtacgg cgggtcaagg acgcctttcc gcacatccag gtcatcggcg
     1621 gcaacatcgt caccggcgac gccgccaggg cgctggtcga ggccggcgcg gacggcgtca
     1681 aggtcggcat cggcccgggc agcatctgca ccacgcgcat cgtcgcgggc gtcggcgtgc
     1741 cgcaggtgag cgcggtggcg aacgtcgccg cggcgctgga gggcaccggc gtgccgctga
     1801 tcgcggacgg cggcatccgc tattcgggcg atctcgccaa ggcgattgtc gcaggcgccc
     1861 attcgatcat ggtcggcggg ctgtttgccg gcaccgagga atcgccgggc gaggtcgagc
     1921 tgtaccaggg ccgctcctac aaggcctacc gcggcatggg ttcgctgggc gccatgtcgc
     1981 agcgccacgg ttccagcgac cgctacttcc aggacgcgac cgacgaggtc gagaagctgg
     2041 tgcccgaggg catcgaggga cgcgtgccct acaagggcag cctgctgggg atcatccacc
     2101 agctcgccgg cggcttgcgt gccgcgatgg gctacacggg ttgctcgacc atcgaggaga
     2161 tgcgcagcaa gccgaagttc gtgcgcatca ccaacgccgg cgtacgcgag agccacgtgc
     2221 acgacgtggc catcaccaag gaagcgccga attaccgcgt ggactgaaga aatcgccgcc
     2281 atgaccaccg acatccacgc gcaacgaatc ctgatcctcg atttcggctc gcagtacacc
     2341 cagctgatcg cgcgccgcgt gcgcgaagcc ggcgtgtaca gcgaaatccg cgcgtgggac
     2401 tgcccggcgg aagacatccg tcacttcgcg ccggcgggca tcatcctgtc cggcggaccc
     2461 gagtcggtga cggtgggcga ggggccgcgc gccgccgccg ccgtgttcga gctcggcgtg
     2521 ccggtgctcg gcatctgcta cggcatgcag accatggccg cccagctggg cggcgaggtg
     2581 gccaactccg cgcaccggga gttcggttac gcgcaggtcc gcatcaccgg ctcgtcgagc
     2641 ctgctcgacg acatcgagga ccacgaggac gcggcgggca gcgcgctgct ggacgtgtgg
     2701 atgagtcatg gcgaccgtgt cgagacgctg ccgccgggat tcatcggcat cgccggcacc
     2761 gacagcgccc cgctggccgg catcgccgac gacggccggc gcttctacgg cctgcagttc
     2821 catcccgagg tcacccacac ccgccagggc gctcgcatcg tgtcgcgctt cgtgcacgac
     2881 atctgcggtt gcggcagcga ctggaacgcc ggcaacatcg tcgaggacag catcgcgcgg
     2941 gtgcgcgcgc aggtcggcgg cggcaaggtg ctgctgggcc tgtcgggcgg cgtggactcc
     3001 tcggtggtgg ccgcgctgct gcaccgggcg atcggtgagc agctcacctg cgtgttcgtc
     3061 gatcacggcc tgctgaggct cggcgagggc gaccaggtca tggcgacttt cgccaggcac
     3121 atgggcatcc gcgtcatccg cgtggatgcc gaggaacgct tcctcgcggc gctggccggc
     3181 gtcaccgacc cggaggagaa gcgcaagatc atcggccggc tgttcatcga ggtgttcgag
     3241 gaacaggcgc aacagctgga ggacgtcgac tggctggcgc agggcacgat ctatcccgac
     3301 gtcatcgagt cggccggcgc cagcaccggc aaggcgcacg tcatcaagtc gcaccacaac
     3361 gtgggcggcc tgcccgagcg catgcgcatg aagctggtcg agccgctgcg cgagctgttc
     3421 aaggacgagg tgcgcaagat cggcctggag ctcggcctgc cctgggacat ggtctaccgc
     3481 catcccttcc ccggtcccgg cctcggcgtg cgcatcctcg gcgaggtccg caaggagttc
     3541 gccgagctgc tgcgccgcgc cgacgacatc ttcatcaccg agctgcgccg ccacgagctg
     3601 tacgaccagg tcagccaggc cttcgcggtg ttcctgccgg tgcgctcggt gggcgtgatg
     3661 ggcgacggcc ggcgctatga ctatgtggtg gcgctgcgcg cggtggagac catcgatttc
     3721 atgacggcgc gctgggcgca cctgccctac gagttcctcg acctggtcgg gcggcgcatc
     3781 atcaacgagg tcgccggcat ctcgcgcgtc acctacgata tttccggcaa gccgcccgcc
     3841 accatcgagt gggagtgagg aacggcgccc tggtgtaagc tggggccgaa cccagcatca
     3901 cgggagggcg ccatggccgg gcccgtcatc gccgccgaca agccgcagaa agtcacgctc
     3961 gaaaagggca aggaatacta ctggtgtcgc tgcggccgct cgcagagcca gcccttctgc
     4021 gacggctccc acaagagcac cgacatcacg ccaaagaagt tcgtcgccga aaaggacggc
     4081 gatgcctacc tgtgcgcctg caagcacacc ggcaaccagc cgttctgcga cggcacccac
     4141 aagcggttct ccgccgagca ggtcggcaag gaaggcccgg gcccgcaggc gattcattcc
     4201 ggggacggcg gccggccgaa ggcggaagcc acgaaggaag agccgactgt ggccttcatc
     4261 catgagctcg cccgggacgg cctgaagtca cacggtgcgc acggccccat gacggcgatg
     4321 ggcgtgccgc gccacgagct gccccattgg gacgacctgc agattctggt ggcgcagctg
     4381 gcggacaagc cgctgcagga ggacgtggcg gtgggcaccg agctggtcat cgggcccgag
     4441 gccaggaaac cgctggtgct gaagattccg ctgttcgtct cggacatgag tttcgggtcc
     4501 ctgtcggagg aagcgaaaat cgcactcgcg cggggcgcgg agctggcggg caccggcatc
     4561 tgctcgggcg agggcggcat gctgcccgag gaacaacagg ccaactcgcg ttacttctac
     4621 gagcttgcca gcgccatgtt cggtttccgg gaggaattgc tggcaagagt ccaggccttc
     4681 cacttcaagg gcgggcaggg cgcgaagacc ggcaccggcg gccacctgcc cgccagcaag
     4741 aacgtcggcc gcatcgcgga ggtccgcggc ctcaccgcag gcgagcccgc ggtctcgccg
     4801 ccgactttcc gggacctgtc ctcgaagaag gatttccgga agttcgccgc gcgggtccgc
     4861 gacatcaccg gcggcgtccc ggtagggttc aagctgagcg ccaaccacat cgaacgcgac
     4921 gtccagttcg cgctcgacgc gggcgccgac tacatcatcc tcgacggccg cggcggcgcg
     4981 accggctcgg cgccgcagat ctttcgcgac cacatcagtg tgcccaccat tcccgcgctg
     5041 gcccgcgcgc gccggcacct ggacaagcag aaggccggcg ggcgcgtcac cctgatcatc
     5101 accggcggcc tgcgcatgcc gatggatttc gtcaaggcca tggcgctcgg cgccgacggc
     5161 gtcgccatcg ccaatgccgc catccaggcc gtgggctgcg tcgcggcgcg catgtgtcac
     5221 accaacaatt gcccctcggg cgtggcgacg cagaaacccg agctgcgcaa gcgcatcgat
     5281 gtcgatcgct ccgcgcggca gctgtgcaac ttcctcgagg catcggtcca gctgatgcag
     5341 gtgatggcac gcgcctgcgg ccacgatcac ctcagccagt tcaatcccgg ggacctcagc
     5401 acctggaagc gggacatggc cctgctgtcc ggggtgaagt tcggcggcgt gctgccgccg
     5461 cgatgagcgt ggctggcttg tccggcacgg tcgacgagct ctacatgcgg cgtgcgctgg
     5521 cgctggctgc ctgcgccgag gccgagggcg aggtgccggt cggagcggtg ctggtggccg
     5581 gcggcgaggt gatcgcggag acctggaacc gctgcatcac gctgagcgat cccgccggcc
     5641 atgccgaggt gcttgcgctg cgcgccggcg ggcaggcgct cggcaactac cggctgcccg
     5701 gcgcaacgct ctacgtcacg ctcgagccct gcgccatgtg cgccggcgcc atggtgcacg
     5761 cgcgcatcgc gcggctggtt tacggcgccg ccgacccgaa gaccggcgcc gccggcagtg
     5821 tgttcgacct cgtgcaggcg cccgcgctca atcatcgcat cgaggtcacc gccggcg
//
LOCUS       DAOMPG010000146         5862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5762_length_5862_cov_3.530394, whole genome shotgun sequence.
ACCESSION   DAOMPG010000146 DAOMPG010000000
VERSION     DAOMPG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5762_length_5862_cov_3.530394"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLS34_08580"
     CDS             <1..302
                     /locus_tag="PLS34_08580"
                     /inference="COORDINATES: protein motif:HMM:NF023592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HRP87561.1"
                     /translation="LGCEAGVVRLVIDPAHEILATSAQQERLRDALAAHFGTTVRLQL
                     EFARPHSETPADRERRAAAERQRQAESAIEEDPLVREMQKVFDATVDRDSIRPAD"
     gene            328..651
                     /locus_tag="PLS34_08585"
     CDS             328..651
                     /locus_tag="PLS34_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRP87562.1"
                     /translation="MKGQIGQLMRQAQQMQENLEKAQQELAALEVTGEAGGGMVRVTM
                     GCKHEVRGIVIDPALLGDDREMLEDLVAAAVNDALRKVERTVQEKYAGMTSGLGLPPG
                     MKLPF"
     gene            659..1255
                     /gene="recR"
                     /locus_tag="PLS34_08590"
     CDS             659..1255
                     /gene="recR"
                     /locus_tag="PLS34_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRP87563.1"
                     /translation="MRYPPRLAGLIEALRRLPGVGPRSAQRMAFHLIEKDREGGAALA
                     AAIEAALEGIGRCGRCRMFTESELCEICRAPTRDAALLCIVETPADVVAVETGSGYTG
                     RYFVLMGHLSPLDGVGPDELGLDRLEAILDEGGIGEVILATNPTVEGEATAHYIAELC
                     TRRGLRASRIAHGVPVGGELEYVDSGTLSHAFAGRQRL"
     gene            complement(1252..2118)
                     /gene="rsgA"
                     /locus_tag="PLS34_08595"
     CDS             complement(1252..2118)
                     /gene="rsgA"
                     /locus_tag="PLS34_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRP87564.1"
                     /translation="MASYGKRLVLEDEAGERRPAFVARRNLKVVCGDRVRWCEAVDGG
                     GEAAVLDILPRATELSRPDSRGRPEVLAANIDQVVVVCAPEPRPDPYIIDRYLAAAEV
                     MGATALVACNKHDLPGASEVVQLAEFEAVGYPTLQVSAHAAHGLDALRNALAGHCSIL
                     VGQSGVGKSSLLNALLPGTGVATAQLSTATGEGRHRTTATTLYPLPGGGALLDSPGVR
                     DFAPALGDPRLAATGFREVVDAAAHCRFANCRHMREPGCAVKDRVATGEISARRYESY
                     RRLLRLTEDLQR"
     gene            2208..2753
                     /gene="orn"
                     /locus_tag="PLS34_08600"
     CDS             2208..2753
                     /gene="orn"
                     /locus_tag="PLS34_08600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRP87565.1"
                     /translation="MKDVDNLIWIDLEMTGLDTARDRVIEIATVVTDKQLNVLAEGPV
                     IAIHQDDDTLAAMDEWNTRQHGKSGLTERVRASRVSEREAELATLTFLSRHAEPGASP
                     MCGNSICQDRRFLARWMPELEKFFHYRNLDVSTLKELARRWAPSVADAFRKESSHLAL
                     RDIYDSIEELKHYREHLLRLP"
     gene            2750..3349
                     /locus_tag="PLS34_08605"
     CDS             2750..3349
                     /locus_tag="PLS34_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRP87566.1"
                     /translation="MTHLPHSAACERNRVPILECIAPLLSTATRVVEIGSGTGQHAAW
                     FAPRLPQLTWVTTDLAANHAGIRGWMEASGAGNLEGPLTLDTLVQPWPELGAIDAAFS
                     ANTAHIMPEPAVVAMFAGLGGRLPSGAPFFLYGPFMEQGRHTSESNARFDAGLRAQDA
                     GMGVRDLGWLLQIAALAGFALEGVAPMPANNRVLVWRRS"
     gene            3334..4308
                     /locus_tag="PLS34_08610"
     CDS             3334..4308
                     /locus_tag="PLS34_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP87567.1"
                     /translation="MAPELIDLPTADDVFAAAERIAPYVRRTPVLTSRGLNAELGTRL
                     FFKCENFQASGAFKARGAFNAVLSLAAAEAVRGVATHSSGNHGAALALAAKRRGIPAW
                     VVVPTDASGFKRDAIARYGAEVVACAPGLASREARLAELVAERGALVVHPYDDNSVIA
                     GQGTATLELLEQAGPLDDLLVPVGGGGLVSGSALAARAVCPGVRIVAAEPAGADDCWQ
                     SMQSGRRVRLEQVNTVADGLRASIGVRNWALISQHVDQVLRIPEQEIVAAMKTAWERL
                     KIVIEPSSAVPLAAILGGHLPVAGRRVGLIFSGGNVDLESLPALLAIR"
     gene            complement(4305..5351)
                     /locus_tag="PLS34_08615"
     CDS             complement(4305..5351)
                     /locus_tag="PLS34_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP87568.1"
                     /translation="MQARLFTFILGMLLLAPSVARAQGAPPAVVRIAQAQVTELAPSI
                     QVPGTVISRNDARISAEVPGRLTWIAEIGSHITAGEPVARIDDAELLLQREEFRGVLA
                     TQESRRGFLAREAARLRQLAAENVAAKNLLDQVETDLQSAESDISTARARLGQIELQL
                     ERTRVRAPFGGVVTERLRTPGEYTQSGDGVVRLVDPASLEVVARAPLASLGFIGPGSE
                     LQLFSDWHSGSGAVRTMVPFGDSRSHMFELRLSIAATPWRVGENVRVAVPSAPAREML
                     AVPRDALVLRREGATVFRITPEGTAEQVEVTAGLSAGELIAVTGRLVAGDRVVIRGAE
                     RLRPGQPVQVIDED"
     gene            complement(5384..>5862)
                     /locus_tag="PLS34_08620"
     CDS             complement(5384..>5862)
                     /locus_tag="PLS34_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005907177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87569.1"
                     /translation="LVIVALPMAMVGGVVTLRITGMLTFQPMDLLTMIGFITLMGLVV
                     NNAILLVAQTRAAEREGIARTAAVEQAVRYRLRPILMSTLTSLFGMLPLLLMPGAGTE
                     LYRGLAAVIVGGLSVSTVFTLVLLPSLLRIGEDAPVAVTDGAGALSGGFAGPERPA"
BASE COUNT          903 a         1985 c         2102 g          872 t
ORIGIN      
        1 acctgggctg cgaggccggc gtcgtgcggc tggtgatcga cccggcgcac gagattctcg
       61 ccaccagcgc ccagcaggag cgcctgcgcg acgccctggc cgcccatttc ggcaccaccg
      121 tgaggctgca gctcgagttc gcgcggccac actcggaaac gcctgcggac cgcgagcgcc
      181 gggcggcggc ggagcgtcaa cgccaggcgg agagcgccat cgaggaagac ccgctggtgc
      241 gggagatgca gaaggttttc gacgccaccg tggatcgcga cagcatccgg ccggcagact
      301 gatcgacttg agcagaggag cagtggaatg aaggggcaga ttggacagct catgcgccag
      361 gcccagcaga tgcaggagaa cctcgagaag gcgcagcagg agctggcggc gctcgaggtc
      421 accggcgaag cgggcggcgg gatggtcagg gtgaccatgg gctgcaagca cgaggtgcgc
      481 ggcatcgtca tcgatccggc gctgctcggt gacgaccggg aaatgctgga ggacctggtc
      541 gcagcggcgg tgaacgacgc gctgcgcaag gtggagcgca cggtgcagga aaagtatgcc
      601 ggcatgacct ccgggctcgg cctgccgccc ggcatgaagc tgccgttcta ggcgtcgcat
      661 gcgttatccg ccgcgactcg cgggcctgat cgaggcgctg cggcgcctgc cgggcgtcgg
      721 cccgcgctcc gcgcagcgca tggctttcca tctcatcgaa aaagatcgcg agggcggggc
      781 cgcgcttgcc gcggccatcg aggccgcgct cgagggcatc ggccgctgcg gccgctgccg
      841 gatgtttacc gagtcggagc tgtgcgagat ctgccgcgcg cccacccgcg acgccgcact
      901 gctctgcatc gtcgagacgc cggcggacgt ggtggcggtg gagaccgggt caggttacac
      961 gggacgctat ttcgtgctga tgggccatct ttcgccgctg gacggcgtcg gtccggacga
     1021 gttggggctc gaccggctcg aagccatcct cgacgagggc ggcatcggcg aggtcatcct
     1081 ggctaccaac ccgacggtcg agggggaagc caccgcgcac tacatcgccg agttgtgcac
     1141 gcgccgcggc cttcgcgcca gtcgcatcgc gcacggcgtc ccggtcggcg gcgaactcga
     1201 gtacgtggac agcggcacgc tgtcgcacgc ctttgccggg cggcaaagac tctagcgctg
     1261 caggtcctcg gtgaggcgca gcagccggcg atagctttcg tagcggcgcg cgctgatctc
     1321 tcccgtcgcg acacgatcct tcacggcgca gcccggctcc cgcatgtggc ggcaattcgc
     1381 gaagcggcag tgtgccgcgg cgtcgaccac ttcccggaag ccggtggccg ccagtcgcgg
     1441 gtcgccgagt gccggggcga aatcccgcac tcccggcgag tccagcagcg ccccgccgcc
     1501 gggcagcgga tagagggtgg tggcggtggt gcggtgccgg ccctccccgg tggccgtgga
     1561 gagctgcgcg gtcgccaccc ccgtaccggg gagcagagcg ttcagcagcg atgacttgcc
     1621 tactccggac tggcccacca ggatgctgca gtggccggcg agcgcattgc gcaaagcatc
     1681 gagcccatgc gctgcgtggg cgctcacctg cagcgtcggg tagccgacgg cctcgaactc
     1741 cgccagctgc accacctcgc tggcgccggg caggtcgtgc ttgttgcagg cgaccagcgc
     1801 ggtggcgccc atgacctccg cggccgcgag gtaacggtcg atgatgtacg gatccgggcg
     1861 cggctcgggc gcgcacacca cgacgacctg gtcgatattg gcggcaagga cttccggccg
     1921 accgcgcgag tcagggcgac tcagctcggt ggcacgaggc aggatgtcga gcaccgccgc
     1981 ctcgccgccc ccatcgaccg cctcgcacca gcgcacccgg tcgccgcaga cgaccttgag
     2041 gttgcgcctg gcgacgaagg cgggacgccg ctcgccggct tcgtcctcga gcaccaggcg
     2101 cttgccgtag gaggccacca cgcggccggt gcacggcgaa ctgtctccgt ccgcatccgg
     2161 cgctctgtta gaattcggaa attgcaagga ggccgcccgc cgcgcttatg aaagacgtcg
     2221 ataacctgat ctggatcgac ctggagatga ccggcctcga caccgcacgg gaccgtgtca
     2281 tcgagattgc caccgtggtc accgacaagc aactgaacgt gcttgccgaa ggcccggtga
     2341 tcgcgattca ccaggacgac gatacgctgg ccgcgatgga cgagtggaac acgcgccagc
     2401 acggcaagtc cggcctgacc gagcgggtgc gcgcgagccg ggtgtccgaa cgcgaggcgg
     2461 agcttgcgac gctgaccttc ctgtcgcggc atgccgagcc gggagcctcg ccgatgtgcg
     2521 gcaacagcat ctgccaggat cgccggttcc tggcgcgctg gatgcccgaa ctggagaagt
     2581 tcttccatta ccgcaacctc gacgtcagca ccttgaagga gctggcgcgc cgctgggcgc
     2641 cgtcggtcgc ggacgcgttc cgcaaggagt cgagtcacct ggcactgcgc gatatctacg
     2701 attccatcga ggagttgaag cactatcgcg agcacctgct gcgcctgccg tgacgcacct
     2761 gcctcattcg gcggcctgcg agcgcaatcg cgtgccgatc ctggagtgca tcgcgccgct
     2821 gctgtcgact gccacgcggg tggtggaaat cggcagcggt acgggtcagc atgccgcctg
     2881 gtttgcgcca cggctgccgc agctgacctg ggtgacgacc gacctggcgg cgaaccacgc
     2941 cggcatccgc ggctggatgg aagccagcgg cgccgggaac ctggagggac cactgacgct
     3001 cgacacgctg gtgcagccct ggcccgagct gggtgcgatc gatgcggcgt tcagcgccaa
     3061 cacggcgcac atcatgccgg agcccgccgt ggtcgcgatg ttcgcggggc tgggtgggcg
     3121 gctgccgtcc ggcgcgccgt tctttctcta cggaccgttc atggagcagg gccgccacac
     3181 cagcgagagc aatgcacgat tcgacgccgg gctccgtgcc caggacgccg gcatgggcgt
     3241 gcgggacctg ggctggctgt tgcagatcgc cgccctggcg gggttcgcac tggagggcgt
     3301 ggcgccgatg ccggccaaca accgggtgct ggtgtggcgc cggagttgat cgacctgccc
     3361 actgcggacg acgtcttcgc cgcggccgag cgaattgcgc cttatgtccg ccgcaccccc
     3421 gtactgacct cgcgcggact gaatgccgag ctcggcacgc ggctgttctt caagtgcgag
     3481 aatttccagg cctccggcgc cttcaaggcg cgcggggctt tcaatgccgt gttgtcgctg
     3541 gcggcggccg aagccgtgcg cggcgtcgcg acgcattcct ccggcaatca cggcgccgca
     3601 ctcgcgctgg cggcaaagag gcgcggaatc ccggcctggg tggtcgtgcc caccgacgcc
     3661 tccggcttca agcgcgacgc catcgcgcgt tacggcgccg aggtggtcgc atgtgcgccg
     3721 gggctcgcca gccgtgaggc ccggctggcc gaactggtcg ccgagcgtgg tgccctggtc
     3781 gtgcatccct acgacgacaa ctctgtcatt gcggggcagg gcaccgcgac gctggaactg
     3841 ctggagcagg ccgggccact ggatgatctg ctggtgccgg tgggcggcgg gggcctggtc
     3901 tcgggcagcg ccctggccgc ccgtgccgtg tgtcccggcg tgcgcatcgt ggcggccgag
     3961 ccggcgggtg cggacgactg ctggcaatcc atgcagagcg gccggcgcgt ccggctggag
     4021 caggtgaaca cggtggcgga cggcctgcgt gcatccatcg gggttcgcaa ctgggcgctg
     4081 atctcgcaac acgtggacca agtcctgcgc attccggagc aggagatcgt cgctgcgatg
     4141 aagaccgcct gggagcggtt gaagatcgtc atcgagcctt catcggcggt gccgctggcg
     4201 gccatcctgg gtggccacct gccggttgcg gggcgccgcg tgggactgat cttctcgggc
     4261 ggcaacgtcg acctggaatc gctgccggcc ctgctcgcca ttcgctagtc ttcgtcgatg
     4321 acctggactg gctgacctgg ccgcagccgt tccgcgccgc gaattaccac ccggtcgccg
     4381 gccacaaggc ggcctgtcac ggcgatgagt tcgccggcac tcagccccgc cgtcacttcg
     4441 acctgttccg ccgtgccctc gggggtgatg cggaacacgg tggcgccctc gcggcgcagt
     4501 accagcgcgt cgcgcggcac cgccagcatt tcgcgtgccg gcgcgctggg caccgcgacc
     4561 cggacattct cgccgacgcg ccatggcgtt gcggcgatac tgagtcgcag ctcgaacatg
     4621 tgcgagcgac tgtcgccgaa gggcaccatg gtgcggacgg cgccgctgcc ggagtgccag
     4681 tcgctgaaca actgcaactc gctgcccgga ccgatgaagc cgagcgaggc cagcggcgca
     4741 cgcgccacga cttcgaggct ggccgggtcc acgaggcgca ccacgccgtc gccggactgt
     4801 gtgtactcgc cgggggtgcg caggcgctcc gtgacgacgc cgccgaaggg ggcgcgcacg
     4861 cgcgtacgct ccagctgcag ctcgatctgg ccgaggcggg cgcgcgccgt cgagatatcg
     4921 ctctcggcgg actgcaggtc cgtctcgacc tggtcgagca ggttcttggc cgcgacgttc
     4981 tccgcagcca gttgccgcag ccgcgcggcc tctcgcgcca ggaaaccgcg gcggctctcc
     5041 tgcgtggcga gcacgccacg gaactcttca cgttgcagca gcagctccgc atcgtcgatg
     5101 cgcgccaccg gctcaccggc tgtgatgtgg ctgccgatct cggcaatcca ggtgagcctg
     5161 ccgggcactt ccgccgagat gcgtgcatcg ttgcggctga tcacggtgcc gggcacctgg
     5221 atgctcggcg cgagctcggt cacctgggcc tgtgcgatac gtaccacggc gggcggtgcg
     5281 ccctgggcgc gagccacgct gggcgccagc agcagcatac ccaggatgaa ggtgaacaat
     5341 ctggcttgca tgggaatctg aactccaggg aaagcttgcg gactcaggca gggcgctcgg
     5401 ggccggcgaa accgccactc agggcgccag cgccgtccgt gacggcgact ggtgcgtcct
     5461 cgccgatgcg cagcaggctg ggcagcagca ccagcgtgaa gaccgtgctg accgagagcc
     5521 cgccgacaat gactgcggcc agcccccggt acagctcggt gccggcgccc ggcatcaaga
     5581 gcagcggcag catgccgaac aggctggtaa gcgtgctcat caggatgggg cgcaggcgat
     5641 agcgcaccgc ctgttcgacc gcggccgtgc gcgcgatgcc ttcacgctcc gcggcgcggg
     5701 tctgcgccac cagcaggatg gcgttgttca cgaccagccc catcaaggtg ataaagccga
     5761 tcatggtaag caggtccatg ggctggaagg tcagcatgcc ggtgatgcgc agcgtgacga
     5821 cgccgccgac catcgccatc ggcagcgcca cgatgaccag ca
//
LOCUS       DAOMPG010000147         5855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5772_length_5855_cov_3.870172, whole genome shotgun sequence.
ACCESSION   DAOMPG010000147 DAOMPG010000000
VERSION     DAOMPG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5772_length_5855_cov_3.870172"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLS34_08625"
     CDS             <1..448
                     /locus_tag="PLS34_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRP87570.1"
                     /translation="ATLWRRLAPAARRLIPPRHAGQALALGALWGWLPCGLVYGMLAA
                     AAASGTARDGALFMAVFGLGTAPAMIGLSWAAGRGGSFLTAQRRRALGWLLVIFGAWT
                     AATPLMRMAGLHGHDPHPPTQEGAAPMHQHHDARAPVTPPAGASIG"
     gene            482..1573
                     /gene="fbaA"
                     /locus_tag="PLS34_08630"
     CDS             482..1573
                     /gene="fbaA"
                     /locus_tag="PLS34_08630"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRP87571.1"
                     /translation="MSQQSAARVSAVARPGVVTGDAVMQVFRLAQAGNFALPAVNVVN
                     SNSVNAVLEAARAVNSPVIIQFSNGGGTFFSGKSLPNDRQQAAILGSIAGARYVHAVA
                     EAYGVPVILHTDHAARKLLPWIDGLLEASRRHFEETGKPLYSSHMLDLSEEPLEENIG
                     TCAAYLERMAPLGMTLEIELGVTGGEEDGVDNTGIDNSALYTQPEDVEYAYRTLSAIS
                     PNFTVAASFGNVHGVYKPGNVLLTPEILKNSQDHVRATHKTPDKPVCFVFHGGSGSEK
                     DKIAEAISYGVVKMNIDTDTQWATWKGVKDYYEAKRDYLQGQIGNPDGDDKPNKKHYD
                     PRVWLREGEKAMVERLREAFADLHCIDRN"
     gene            complement(1595..2071)
                     /locus_tag="PLS34_08635"
     CDS             complement(1595..2071)
                     /locus_tag="PLS34_08635"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HRP87572.1"
                     /translation="MRYLPALLAAALLLAGCEEKQPMTVADFMGNEAALYGTLTRCQE
                     NPSAGTDPECRNARQAAERISVIEERAMRKAREEAFASARAEYRAQLDRERELRLKAE
                     AEAEEARLQSLLSPAPEDVESGEEHGPVNGSNGTTAADGAADAAVEALTPEPDDSR"
     gene            complement(2147..5620)
                     /locus_tag="PLS34_08640"
     CDS             complement(2147..5620)
                     /locus_tag="PLS34_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRP87573.1"
                     /translation="MRLELPDFRFDEVLPLAEGLDMPPGDAERALYRILYWTGGHPYL
                     TQKLCQAVARNAARINSDAAVDRLVAARFFARNAVVSETSMSRVLDGLDRAGKLARPA
                     LRLYRRIRRGRRPRYEPGNSEHELLRVCGLVKLNGERRLVVRNRIYAEVFTHRWAREA
                     LPVEWRRVGRAATISVLLAAALWGYAEVLPRPYDETLRVASVELDEAVEAWSSLRRIP
                     GFGGRADRLLSRVLIRGSRQATRWPEAADLDERLRGLSGYEARADALLVEYWERRAAE
                     AEAAERRDAALLYRLRAYHAGPTADAGLVAELSGGGYRQLETVIRPAGTVDALAAADA
                     GRRIVTVSAGNVVEQWHADTGLPAAGARLELLAEEFVTVRRRLSLDTSGRVAQVRAEL
                     ELEHPRPGDLYLRLTAPSGRSVELPLPDAAVDGSRVLIDERRAPGLRALRGEPVLGTW
                     LLEIEDRVGGSPGFLRGWVLQASPAAGHRAEDRPENALLLPAPSRTSAVRSALSPGGS
                     TVAALPRNAQARGRLQTWDVAGARALASVPVGAGERWIGFADEETLLLVAGVGAGEHL
                     RVLDATSGAERFVHRSEGRFVAGPAVSPDGQFVAVAEATPQAAWIRALDSGREIFRLP
                     AAGEVTAVSVGPGGLMLAVADRGNVVRVWHSSDGALAAEYPQDSTVAEVAFDPSGRWL
                     VTVDAARQVRVWDLSTPGAVPVLARRGGETRQFGFDGSGRRFAMQGPAGGYEIWALQE
                     GLPAAPVLPHPAEQAATSGPPERPSAARLVLAGDGRLIGGQGTRSVRSWDTARAGSAT
                     ELPRIAPVVALAPSGLRMAAGMSDGRVILRMRDPDTLVLQQALVTSGETRHGGAVTAL
                     DFSPDSGRLASVGADGSVLLWDAATGRPLGGLFQHGSGRVMAVDLGPDGRRLVTAGEL
                     GARSWDAQAGTPGPDLGPGRLATAVALDPAGLRAYTGTAAGEVESWDVASGERLWFGD
                     IGAPVSRLAVSTDGARIAAASDAGLVQSWGMGSAGRPLSTDLPAPVIGLRFSPDGKAL
                     IAQTASWMHRLEAADGRLRVTASRLLPAAVPPGAWRSAGADGMRIVLVGGAHSETMAV
                     LDFQRAPSPPEDWQADLGGWQRKLKLYFTTDGELQAGIPPAPPAQDAEAAAESIEPAA
                     DDQSEAGPPPQ"
BASE COUNT          901 a         2190 c         1948 g          816 t
ORIGIN      
        1 cgcaaccctg tggcgcaggc tcgccccggc cgcacgccgc ctcatcccgc cgcggcatgc
       61 cggccaggcg ctggcgctgg gcgcactgtg gggctggctg ccctgcggcc tggtgtacgg
      121 catgctggcg gctgccgcgg ccagcggcac tgcgcgcgac ggcgcgctgt tcatggcggt
      181 gttcggcctc ggcacggcgc cggcgatgat cggcctgagc tgggcggccg ggcgcggtgg
      241 cagttttctt acggcgcagc gccggcgcgc gctcggctgg ctgctggtga tcttcggtgc
      301 atggaccgca gccacgccgt tgatgcgcat ggccggactg cacggccatg acccgcaccc
      361 gccgacccag gagggcgcag caccgatgca ccagcaccac gacgcccggg cgccggtcac
      421 acccccggcc ggggcctcga tcggctagaa tttcgcgctg ttttcgcagg agcactgatt
      481 catgtcgcaa caatccgccg cccgcgtatc tgctgtagcc cgccccggcg tcgtcaccgg
      541 cgatgccgtc atgcaggtct tccggcttgc acaagccggc aactttgcct tgccggccgt
      601 caacgtcgtc aactccaact cggtaaacgc cgtgctggaa gcggcccggg cggtgaactc
      661 gccggtgatc atccagttct ccaacggtgg cggcacgttt ttctccggca agtcgctccc
      721 caacgacagg cagcaggccg ccatcctcgg ctcgatcgcg ggcgcgcgct atgtgcatgc
      781 ggtggcggag gcctacggcg tgccggtgat cctgcatacc gaccatgccg cgcgcaagct
      841 gctgccatgg atcgacggcc tgctggaggc cagccgcagg cacttcgagg agaccggcaa
      901 accgctctac tcgtcgcaca tgctggacct gtcggaggag ccgctggagg aaaacatcgg
      961 cacgtgcgcc gcatacctcg agagaatggc gccgctcggc atgacgctgg agatcgaact
     1021 cggcgtgact ggcggcgagg aagatggcgt ggacaacacg ggcatcgaca actcggccct
     1081 gtacacccag ccggaggacg tcgagtacgc ctaccgcacg ttgtcggcca tcagccccaa
     1141 cttcacggtg gccgcctcat tcggcaacgt gcacggcgtc tacaagccgg gcaacgtact
     1201 gctcacgccg gaaatcctga agaactcgca ggaccacgtg cgcgccacgc acaagacgcc
     1261 cgacaagccg gtctgtttcg tgttccacgg cggctccggc tcggagaagg acaagatcgc
     1321 ggaagcgatt tcctacggcg tggtgaagat gaacatcgac accgacacgc agtgggccac
     1381 ctggaagggc gtgaaagact actacgaggc gaagcgcgac tacctgcagg gccagatcgg
     1441 caacccggac ggcgacgaca agcccaacaa gaaacactat gacccgcgcg tctggctacg
     1501 cgagggcgag aaggcgatgg tcgagcggct cagggaagcg ttcgcggacc tgcactgcat
     1561 cgaccgcaac tgatcgcgcc ggacggcggc cgcttcagcg gctgtcgtcc ggctcgggcg
     1621 tcaacgcctc aacggcagca tcagcggcgc catccgccgc ggtcgtaccg ttgctcccgt
     1681 tcaccggccc atgttcctcg ccgctctcca cgtcctcggg cgccgggctg agcaaggact
     1741 gcaaccgggc ctcctccgcc tcggcttcgg ccttcaggcg cagctcccgc tcccgatcga
     1801 gctgggcgcg gtactccgct cgcgcgctcg caaacgcttc ttcgcgcgcc ttgcgcatcg
     1861 cccgctcctc gatcaccgag atcctttccg ccgcctggcg cgcgttgcgg cactcggggt
     1921 ccgtgccggc agagggattc tcctggcaac gcgtgagcgt gccgtacagc gccgcctcgt
     1981 tgcccatgaa atcggccacg gtcatcggct gtttttcctc gcatcccgcg agcaacagcg
     2041 cggccgccag cagtgcgggc aagtacctca tgacgatttc ccccggacgt tcaaaaagca
     2101 gattctagcc gctcccgcgc atgccgaggt ttgcgccgcg tcacaatcac tgcggtggcg
     2161 gcccggcttc ggactggtcg tcggccgcag gttcgatcga ctccgcggcg gcttcggcgt
     2221 cctgcgcggg cggcgccggc ggaatgcccg cctgcagttc tccgtcggtg gtgaagtaca
     2281 gcttcagctt ccgctgccag ccacccaggt ccgcctgcca atcctcaggc ggcgacggtg
     2341 cccgctggaa atcgagcacg gccatggtct cgctgtgcgc gccgcccacc agcacgatgc
     2401 gcatgccgtc cgcgccggca ctacgccacg cgcctggcgg cacggccgcc ggcagcagcc
     2461 ggctcgcggt cacccgcagg cggccgtccg cggcttccag gcgatgcatc caggatgccg
     2521 tctgtgcgat caatgccttg ccgtctgggg agaaacgcag cccgatgacc ggcgcaggca
     2581 ggtctgtact cagcggccgc ccggcgctgc ccatgcccca ggactgcacc agcccggcgt
     2641 cgctggccgc cgcgatcctg gcgccgtcgg tgctcacggc gagccgggac accggcgcac
     2701 caatgtcgcc gaaccagagg cgctcgccgc tcgcgacgtc ccagctttcc acttcgccgg
     2761 ccgcggtgcc tgtgtaggcc cgcagtcctg ccggatcgag cgccacggcg gtcgccagcc
     2821 gccccggacc caggtcaggc cccggggtgc cggcctgggc atcccaggag cgtgccccca
     2881 gttcgcccgc cgttaccagc cgcctgccat ccgggccgag atcgaccgcc atcacgcggc
     2941 cgctgccgtg ctggaacaat ccgccgagcg gccggccggt cgcggcatcc cagagcaaca
     3001 cgctgccgtc ggccccgacg ctggccagtc gcccgctgtc gggggaaaag tccagcgccg
     3061 tcaccgcgcc gccatggcgt gtctcgccac tggtcaccag ggcctgctgc aggaccagcg
     3121 tgtcgggatc gcgcatccgc aagatgacgc ggccgtccga catcccggcc gccatgcgca
     3181 ggccggaggg cgcgagcgcg acgaccggcg caatgcgcgg caactcggtg gcggaacccg
     3241 cgcgcgcggt gtcccagctg cgaacactgc gtgtaccctg tccgccgatc aggcggccgt
     3301 cgccggccaa caccaggcgg gcggccgacg gccgttcggg cgggcccgat gtggcggcct
     3361 gctccgcagg atgcggcaac accggagcgg cgggcaggcc ttcctgcagc gcccagattt
     3421 cgtaccctcc cgcgggcccc tgcatggcga accggcgccc tgatccatcg aagccgaact
     3481 gccttgtctc gccgccgcgc ctggccagga cgggcaccgc cccgggcgtc gacaggtccc
     3541 acacccgaac ctggcgggcc gcgtccaccg tcaccagcca tcgtcccgaa gggtcgaagg
     3601 ccacttcggc cacggttgaa tcctgtggat actccgccgc cagcgcgccg tccgagctat
     3661 gccagacccg caccacgttg ccgcgatccg caaccgcgag catcaggccg cccggtccga
     3721 cggacactgc ggtaacttcg cccgccgccg gcaaccggaa tatttcacgg cccgaatcca
     3781 gcgcgcggat ccaggcagcc tggggcgtgg cctcggccac ggcgacgaac tgaccgtccg
     3841 gcgacactgc cgggcccgcc acgaagcgcc cctcgctgcg atgcacaaag cgctccgcac
     3901 cgctggtggc atcgaggacc cgcagatgtt cgccggcacc cacaccggcc accagcagca
     3961 gcgtctcctc gtcggcaaag ccgatccagc gctctccggc gccgaccggt acactggcca
     4021 gcgcccgggc gccggcgacg tcccaggtct gcagacgccc acgtgcctgc gcattgcgcg
     4081 gcagggccgc gaccgtcgag ccgcccggcg atagcgcact gcgtaccgcc gaggtccgcg
     4141 acggcgccgg cagcagcagg gcgttctccg gccggtcctc ggcgcgatgc ccggcggcgg
     4201 gcgaggcctg cagcacccag ccgcgcagaa accccggcga tccgccgacc cggtcctcga
     4261 tttccagcaa ccaggttccc agcacaggct cgccgcgcaa ggcgcgcagg cccggcgcac
     4321 ggcgctcgtc gataagaacc cggctcccat ccacggcggc gtccggcaac ggcagctcca
     4381 cggatcgtcc ggacggcgcg gtcagccgca ggtacaggtc gcccggccgc gggtgctcca
     4441 gctccaactc cgcgcggacc tgcgcgaccc ggccgctggt atcgaggctc aaccgccggc
     4501 gcaccgtcac gaattcctcg gccagcaatt ccagccgggc accggctgcc ggcaacccgg
     4561 tgtccgcgtg ccactgctcg accacgttgc cggcgctgac ggtcacgatg cgtcggcccg
     4621 cgtccgcggc cgcaagcgcg tcgaccgtcc cggccggacg gatcacggtc tcgagctgcc
     4681 gatacccgcc accggacagt tcggcgacca ggccggcgtc ggcggtcgga cccgcatggt
     4741 aggcacgcag gcggtacaac agggcggcat cgcgccgctc ggccgcctcg gcttccgccg
     4801 cacgccgctc ccagtactcg accagcagcg catcggcccg ggcctcgtag cccgacaggc
     4861 cgcgcagccg ctcgtcaagg tcggcggctt ccggccagcg ggtcgcctgc cggctgccgc
     4921 ggatcagcac gcgcgacagc aggcgatcgg cacggccgcc gaagcccggg atgcggcgca
     4981 acgaggacca ggcctcgacc gcctcgtcca gctcgaccga ggcgacgcgg agggtctcgt
     5041 cgtagggacg cggcaggacc tcggcgtagc cccagagggc ggcggcgagc agcacgctga
     5101 tcgtggcggc acggccgacg cgccgccatt ccacgggcag ggcctcgcgg gcccagcggt
     5161 gggtgaacac ctcggcataa atccggttgc gcaccaccag ccggcgttcg ccgttgagct
     5221 tgaccagccc gcagacgcgc agcagttcgt gctcggaatt gccgggctcg taacgcggcc
     5281 ggcggccacg ccgaatccgc cgatagagcc gcagcgccgg cctggccagc ttgccggcgc
     5341 gatccagccc gtcgagcacc cggctcatgc tggtctcgct caccaccgcg ttgcgggcaa
     5401 agaatcgcgc ggcaacgagc cggtccacgg ctgcgtccga attgatgcgc gcggcgttgc
     5461 gcgccaccgc ctggcagagt ttctgggtga gatagggatg cccacccgtc cagtacagga
     5521 tccggtacag cgcacgttcc gcgtcgcccg gcggcatgtc gaggccctcg gcaagcggca
     5581 gtacctcgtc gaaacggaaa tccggcagct cgagtcgcac gccgatctcc gccggggcga
     5641 taccggaccg cgctctatgg agcagactgg cagcatggcc ggattctgat gatcgacatg
     5701 gtcccgcagg gggcctctta tcgcggccag atgtctgctt ttctggaagg cagcccgctg
     5761 aatgtctgcg atctgacgtt tggacccgat ggagccatgt acttcatcac cggtggacgg
     5821 ggatcacagt ccggattgta ccgggtcacg ttgac
//
LOCUS       DAOMPG010000148         5801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5846_length_5801_cov_4.155761, whole genome shotgun sequence.
ACCESSION   DAOMPG010000148 DAOMPG010000000
VERSION     DAOMPG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5846_length_5801_cov_4.155761"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3041
                     /gene="smc"
                     /locus_tag="PLS34_08645"
     CDS             <1..3041
                     /gene="smc"
                     /locus_tag="PLS34_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRP87574.1"
                     /translation="KPEELRMYLEEAAGISRYKERRRETETRIRHTRENLDRLNDLRE
                     EVEKQIRHLQRQARTAERYREMKSEQRRLEAELLVLRLAGLEAEAGAQKSALGERENT
                     LQAAVAELRRVEADLEAARERHREEGEALNAVQGDYYRIGAEIARLEQAIQHARELRN
                     RQSQELEQSEQGMLEIRQHIERDSDQLAELNRVLGTLGPELDAARAAEARSAEALRSA
                     EQAMQDWQASWEGFNREASDAREMVEVERARIEQLEGQITRLAERRRRIEEERSRLAA
                     EAGDEDLDALALELDRARELEQSTQAQDVAVRDEIVQLREQDQQLVEQLDKGRSALQT
                     LRGTLASLEALQRAAVGRRGDQVGKWLEGQGLAERPRLAERLSVEPGWERAVETVLGQ
                     YLEAVCVDEIDAIATELDSLAQGAASFLEVTGGDGGSGDTALLASRVKGVQGSTALLG
                     GVYAVDDLGAALARRRTLAPTESVITRDGIWLGPNWLRVNRAEDPHSGVLAREQEIRE
                     LRASIGGAAYEVEELAARHEEIRTRLAMLEASRDEVRQQAADAHRTAAGLQATLQTAR
                     SRLEQAANRLAGLEQEAGEVETQLAEADTAIRGSRGRLQEGIELMSGVEGRRQGLEAE
                     RERLLTALQDVRDQARTDRSAAQEIAIRVESRRSSLDSASLSMQRFQDQVARFAARKE
                     ELERQLEAGVEPLAERQATLEGLLAQRLEVEESLAAARRKVQALEAELREFQEQRTGR
                     DQVVDRLREELEGLRLSAREVSVRLQTAVEQFAATGFELETLRAELPEDADMPAWEER
                     LAQLAARIERLGPINLASIDELREQSERKQYLDAQLADLNDALGTLENAIRRIDRETR
                     TRFKETFDRVNAGMQELFPRLFGGGNAFLQLESEDLLEAGVTVMARPPGKRISTIHLM
                     SGGEKALTAVALVFAIFQLNPAPFCMLDEVDAPLDDANVGRFCDIVRTMSEKVQFIII
                     THNKTTMEMVNQLTGVTMHEPGVSRLVAVDIDEAVQMVAS"
     gene            3059..3772
                     /locus_tag="PLS34_08650"
     CDS             3059..3772
                     /locus_tag="PLS34_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HRP87575.1"
                     /translation="MPELRWILTAIGVALIAGVYVWSRRDRAGDEPDAHRREPAIGAR
                     SEGWHGAPPTPRPAPEPMLGHDPLLDDVPADELPGDDVPVIPPEGEQLIVALRLRARE
                     PAGFDGNDLIKAFSAESLEFGKFGAFHSLDERRRSRFLVASLVEPGSFEIEKMHELRC
                     PGVSMFMVLPGPREPLAALDAMFVCARRLAERLDADVLDDKGNALTMQQAGWLREQVV
                     EFHRRSKLGAGTGRPGDLP"
     gene            3775..5799
                     /gene="ligA"
                     /locus_tag="PLS34_08655"
     CDS             3775..5799
                     /gene="ligA"
                     /locus_tag="PLS34_08655"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRP87576.1"
                     /translation="MAKVDPAVRAAELRKLIAHHDYRYYVLDDPEIADVDYDRLFREL
                     QAIEAAHPELCTEDSPTQRVGGAPVARFGTVEHLLPMLSLDNAFTSDELIAFDRRASE
                     RLKTEETLEYSAETKLDGTAISLLYEHGRLVRGATRGDGQTGEDVTHNVRTIESIPLR
                     LIGRKHPAVLEVRGEVYMPKAGFAALNRRLARDGAKTFVNPRNAAAGTLRQLDPRITA
                     RRPLEFFCYGIGYVEGGEVPARHSEILAHLREWGIRVSPLSRVVRGVEGCQAYYDEVA
                     ALRDTLPYEIDGIVLKVDRIDQQQALGYVSRAPRWAIAFKFPAQEVTTVVRGVDFQVG
                     RTGALTPVARLEPVFVGGVTVSNATLHNIDEIERKDVRVGDTVIVRRAGDVIPEVVRV
                     LLEQRQGNPHKVRLPKTCPVCGADVVRPEGQSVARCSGGLVCAAQRKEKLRHFASRRA
                     MDIEGLGAKLVEQLVEQGAGERPIKTPADLYTLTTAELASLDRMGQKSAENLVAAIER
                     SKSTTLPRFLFALGIPEVGEATAANLARYFGDLGPLAQAEIEELEAVPDVGPVVARHV
                     HDFLRQEENLAVIEALRKHGVSWPVAKATKSGEQPLMGKTFVLTGTLESMTRDEARER
                     IEQAGGKVTGSVSSKTDYVVVGADPGSKLKKAQELGIEVVDEAGLGRLIV"
BASE COUNT         1029 a         1829 c         2123 g          820 t
ORIGIN      
        1 ccaagcccga agagcttcgc atgtacctcg aggaggcggc gggtatttcc cgctacaagg
       61 agcgccgccg cgagaccgag acgcgcatcc gccacacgcg cgagaacctc gaccggctca
      121 acgacctgcg tgaggaggtc gaaaagcaga tccgccacct gcagcgccag gcacgcaccg
      181 ccgagcgcta tcgcgagatg aaatccgaac agcgccgcct cgaggccgag ttgctcgtgc
      241 tgcgcctcgc cgggctcgag gcggaggccg gtgcgcagaa atcggcgctg ggtgagcgtg
      301 aaaacacgct gcaggccgcg gtcgccgagc tgcggcgggt cgaggcggac ctggaggcgg
      361 cgcgcgagcg gcatcgcgag gaaggcgaag cgctgaatgc cgtgcagggc gactattacc
      421 ggatcggagc ggagatcgcg cggcttgagc aggccatcca gcacgccagg gaactgcgca
      481 accgccagtc gcaggaactc gagcagtccg agcaaggcat gctggagatt cgccagcaca
      541 tcgagcgtga ctccgaccag ctggccgagc tgaatcgcgt gctgggaact ctggggccgg
      601 agctggatgc cgcgcgcgcc gccgaggcgc ggtcggctga agcgctgcgc tcggccgagc
      661 aggcgatgca ggactggcaa gcaagctggg agggcttcaa ccgcgaggcc agcgacgccc
      721 gcgaaatggt cgaagtcgag cgcgccagga tcgagcagct cgaagggcag atcacccgcc
      781 tcgccgagcg ccgccggcgc atcgaggagg agcgcagccg cctggccgcc gaggccggcg
      841 acgaggacct cgacgcgctt gcgctcgagc tcgatcgggc ccgcgagctc gagcagagca
      901 cccaggcgca ggacgtcgcg gtgcgcgacg agatcgtcca gctgcgtgag caggaccagc
      961 agctcgtcga gcagctggac aagggccgca gcgccttgca gacgctgcgc ggcacgctgg
     1021 cgtcgctcga agcgctgcag cgggccgcgg tcgggcgccg gggcgaccag gtcggcaagt
     1081 ggctggaggg gcaggggctg gccgaacgcc cgcggctggc cgagcggctg tcggtggaac
     1141 ccggctggga gcgcgccgtc gagacggtgc tgggccagta cctcgaggcg gtgtgtgtgg
     1201 acgagatcga cgccatcgca accgagctgg acagcctggc gcagggcgcc gcgagctttc
     1261 tcgaggtcac ggggggcgac ggtgggagcg gcgacacagc cctgctggcg agccgtgtca
     1321 agggcgtgca gggctcgacg gccttgctgg gcggcgtgta cgcggtcgac gacctgggcg
     1381 cggcgctggc gcggcgtcgc acgctggcac cgaccgagtc ggtcatcacg cgggacggca
     1441 tctggctcgg gcccaactgg ctgcgagtga atcgcgccga ggacccgcat tccggtgtgc
     1501 tggcccgcga gcaggagatt cgcgaactgc gcgcctccat tggcggcgca gcctacgagg
     1561 tcgaagagct ggcagcgcgg cacgaggaga ttcgcacccg gcttgccatg ctcgaggcct
     1621 cgcgcgacga ggtgcggcag caggccgccg acgcccatcg caccgccgcc ggactgcagg
     1681 cgacattgca gaccgcgcgc tcgcgtctcg agcaggccgc caaccggctt gcagggctcg
     1741 agcaggaagc gggcgaggtg gagacccagc tggccgaggc cgacacggcc atccgcggca
     1801 gtcgcggtcg gttgcaggaa ggcatcgagc tgatgagcgg cgtcgagggc cgtcggcaag
     1861 gcctggaggc cgagcgggaa cgcctgctga cagcgcttca ggacgtgcgg gaccaagcgc
     1921 gcacggaccg atccgccgcg caggaaatcg ccatccgggt ggagtcgcgg cgttccagcc
     1981 tggactcggc cagcctcagc atgcagcgct tccaggacca ggtggcgcgc tttgcggcgc
     2041 gcaaggaaga gctggaacgg cagctcgagg cgggcgtgga gccgctcgcc gagcggcagg
     2101 cgaccctgga ggggctgctg gcgcaacggc tggaggtcga agagtcgctg gccgcggcgc
     2161 ggcgcaaggt gcaggcgctg gaagccgagt tgcgcgagtt ccaggagcag cgcacggggc
     2221 gcgatcaggt cgtggatcgc ctgcgtgagg agctggaggg gctgcggttg agcgcgcgcg
     2281 aagtgagcgt acggctgcag acggccgtcg agcagtttgc cgcgacgggc ttcgaactgg
     2341 agacgctgcg cgcggagctg ccagaggacg cagacatgcc ggcctgggag gagcgcctcg
     2401 cacagctggc ggcgcgcatc gagcgcctcg gtcccatcaa cctggcatcg atcgacgaac
     2461 tgcgggagca gagcgagcgc aagcaatacc tggacgccca gctggcggac ctgaacgatg
     2521 cgctcgggac gctcgagaat gcgatccgac ggatcgaccg cgagacgcgc acccgcttca
     2581 aggagacctt cgatcgcgtc aacgcgggga tgcaggagct tttccccagg ctgttcggcg
     2641 gcggcaacgc cttcctgcag ctcgaatcgg aggacctgct cgaggccggc gtcaccgtga
     2701 tggcgcgacc acccggcaag cgcatcagca ccatccacct gatgtcgggc ggcgagaagg
     2761 cgctcacggc cgtggccctg gtgttcgcca tttttcagct gaacccggcg ccgttctgca
     2821 tgctcgacga ggtcgatgcg ccgctggacg acgcgaacgt cgggcggttc tgcgatatag
     2881 tcaggacgat gtcggaaaag gtccagttca tcatcatcac gcacaacaag accaccatgg
     2941 agatggtcaa ccagttgacc ggcgtgacga tgcacgagcc cggtgtatcg cgactggtcg
     3001 cggtggacat cgacgaggcg gtccagatgg tcgccagctg accagccagg agatccaggt
     3061 gcccgagctt cgctggatac tgacggcaat tggtgtggcc ctgatcgccg gggtctatgt
     3121 ctggagccgt cgcgaccggg ccggcgacga gccggatgcc catcggcgcg aaccggccat
     3181 cggcgcgcgc agcgagggct ggcacggcgc cccgcccacg ccgcggcccg ctcccgagcc
     3241 catgctcggt cacgacccgc tgctcgacga cgtcccggcg gacgaactcc ccggggacga
     3301 cgtgcccgtc attcccccgg agggcgagca gctgatcgtg gccttgcgtt tgcgcgcgcg
     3361 cgaaccagcc ggcttcgacg gcaacgacct gatcaaggcc ttctccgcgg agagcctgga
     3421 gttcggcaag ttcggcgcgt tccactcgct cgacgagcgc aggcgcagcc gctttctggt
     3481 cgccagcctg gtcgagccgg gcagcttcga gatcgagaag atgcacgagc tgcgctgccc
     3541 cggcgtttcc atgttcatgg tgctgccggg tccgcgcgag cctctcgccg cgctggacgc
     3601 catgttcgtt tgcgcacgcc ggctggcgga acggctcgat gccgacgtcc tggacgacaa
     3661 gggcaacgcc ctgaccatgc aacaggccgg ctggttgcgt gaacaggtgg tcgagttcca
     3721 tcgccgcagc aagctcgggg cgggcacggg gaggcccggg gatctgccgt gagcgtggcg
     3781 aaggtggacc cagcggtgcg cgccgccgag ttgcgcaagc tcatcgcgca tcacgactac
     3841 cgctattacg tgttggatga tccggagatc gcggacgtcg actatgatcg gctgttccgc
     3901 gagttgcagg ccatcgaggc cgcgcacccc gaactgtgca ccgaggattc gcccacccag
     3961 cgcgtgggcg gtgcgcccgt cgcgcggttc ggaaccgtcg agcacctgtt gccgatgttg
     4021 tccctcgaca acgcctttac gagcgacgag ctcatcgcat tcgaccggcg cgccagcgaa
     4081 cgactcaaga ccgaggagac gctggagtac tcggccgaga ccaagctcga cggcaccgcc
     4141 atcagcctgc tgtacgagca cggccggctg gtgcggggtg cgacccgggg tgacgggcag
     4201 accggcgagg acgtcacgca caacgtccgg acgatcgagt ccattccgct gcgcctgatc
     4261 ggccggaagc acccggcggt cctcgaggtc cgcggcgagg tctacatgcc caaggccggg
     4321 ttcgcggcac tcaaccgacg gctcgccagg gacggcgcca agactttcgt gaatccgcgc
     4381 aacgccgcag ccggcacgct gcggcagctg gacccgcgaa tcaccgcccg gcgtccactg
     4441 gagttcttct gctacggcat aggctacgtc gaaggtggtg aagttccggc gcgccacagc
     4501 gaaattcttg cccacctccg ggaatggggc atccgggttt caccgctcag ccgggtcgta
     4561 cgaggggtgg agggttgcca ggcttactac gacgaggtcg cggcactgcg cgataccctg
     4621 ccgtacgaga tcgacggcat cgtgctcaag gtcgaccgta tcgatcagca acaggcgctg
     4681 ggctacgtgt cacgggcgcc gcgatgggcc attgcgttca agtttccggc gcaggaagtg
     4741 acgaccgtag tgcggggcgt ggacttccag gttggacgca ccggcgccct gacaccggtg
     4801 gcgcgactcg agccggtgtt cgtgggcggc gtcaccgtca gcaacgccac gttgcataac
     4861 attgacgaga tcgagcggaa ggacgtgcgc gtcggcgata cggtgatcgt gcgccgggca
     4921 ggcgacgtga ttccggaggt cgtcagggtg ctgttggagc aacggcaggg gaatccacac
     4981 aaggtccgac taccgaaaac ctgtcccgtc tgtggcgccg acgtcgtccg cccggagggc
     5041 cagtccgtgg cgcgttgttc cggtgggctg gtatgcgcag cgcaacgcaa ggaaaagctg
     5101 cgccacttcg catcgcggcg cgccatggac atcgaggggc ttggcgcgaa gctggtggag
     5161 cagctcgtgg agcagggcgc aggtgagcgg ccgatcaaaa cgccggccga tctctataca
     5221 ttgaccacgg cagaactcgc cagcctggat cggatgggcc agaagtccgc cgagaacctg
     5281 gtggcggcca tcgagcgtag caagagcacg actttgccca ggttcttgtt tgcgctcggt
     5341 attcccgagg tgggtgaggc gactgcagca aatctggcgc gatatttcgg tgacctggga
     5401 ccgctcgccc aggcggagat cgaggagctc gaagctgtcc ccgacgtggg accggtggtc
     5461 gccaggcacg ttcatgattt cctgcgccag gaggagaacc ttgcagtcat cgaagcgttg
     5521 cgtaagcatg gtgtgagctg gccggtggca aaggcaacga agtccggcga gcagccgtta
     5581 atgggcaaga cattcgtgct caccggaacc cttgaatcga tgactcgaga cgaggcccgg
     5641 gagcgaatag aacaggccgg cggcaaggta acaggaagcg tctcgtcaaa gacagactac
     5701 gtggtggtcg gggccgatcc gggctcaaag ctcaagaagg cacaagagct cggcatcgaa
     5761 gttgtcgatg aggctgggtt ggggcggtta attgtgtaag c
//
LOCUS       DAOMPG010000149         5772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5886_length_5772_cov_4.874410, whole genome shotgun sequence.
ACCESSION   DAOMPG010000149 DAOMPG010000000
VERSION     DAOMPG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5886_length_5772_cov_4.874410"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..751
                     /locus_tag="PLS34_08660"
     CDS             398..751
                     /locus_tag="PLS34_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87577.1"
                     /translation="MDEDPLLLMRQAEAAVLAVTRYLAAQDGPPPAWRDAPTGHAAAD
                     IRRRLLARNDGPVATHLALQSPNWTLGTRDASVLGPYRLDDRQGLLETGFLHVELARS
                     GRQWRVAAVRLDPSP"
     gene            748..>1286
                     /locus_tag="PLS34_08665"
     CDS             748..>1286
                     /locus_tag="PLS34_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87578.1"
                     /translation="MSTLRWLLFGALLAALVAAGAARLATHDDASRLQADLAAAEAAL
                     RQEDSAHAVALARGVLEREPVNGHAFGVLARALADDGNDQADRLRFKAAARRAPRDPG
                     VRAKLAAWALEAGDHATAVDHLDALLTGAPGQRQPMLQLIASMTPDRDFSAALAAHLI
                     RYPQWRSAILRATTASKLP"
     assembly_gap    1287..1296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1297..1979
                     /locus_tag="PLS34_08670"
     CDS             <1297..1979
                     /locus_tag="PLS34_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87579.1"
                     /translation="RSAILHASVVHGSGEGADHVHGAMRAHGGLSAAETARWIDGMLR
                     DGRWGSAYARWVSGLDDAPERLPVPWNGDFGREPSSSGFDWRVRRTPGVLFDRVALDG
                     GGHAARLRFLGRPVARPGLEIPLLLAPGDHVLRLRAGSDGLRSDQGLAWSLSCGDGRT
                     RIAEGARLREAAAWTPLELAFTVPDTDGCEGQWLRLLNPAPRGVGQTLRGEIQLTDVV
                     IVRAAASP"
     gene            2076..2501
                     /locus_tag="PLS34_08675"
     CDS             2076..2501
                     /locus_tag="PLS34_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87580.1"
                     /translation="MNGITHLLAAAILASLPLTVAAQAADPAPAAPIALEIETGSVLV
                     STGGEFGPAAAGQGLQPGHRVLVGEGASAVLRYPDGCTFALREPGVHPVASQCQPART
                     PSMAPRGSTIAMVGVGVLAVGALAGGGGSDSTPQRPISR"
     gene            2790..3218
                     /locus_tag="PLS34_08680"
     CDS             2790..3218
                     /locus_tag="PLS34_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87581.1"
                     /translation="MHQRLLFTTALVSALAAAPVLAQSSAATLRIDSGSAMVSTGGEF
                     TTAGSGAQVPGGSRLMLTEGSSATLVYPNGCTKRFESAGVHNVTGTCSLAASGGSGAG
                     GAGVDWTGAGLIAGGTVLVGAVLANQDDVEIQAPVPPVSR"
     gene            3601..4986
                     /locus_tag="PLS34_08685"
     CDS             3601..4986
                     /locus_tag="PLS34_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRP87582.1"
                     /translation="MTPLDQQARAGLLAVAMLLVLCLATGGSSQESGSGVFAAQLLSL
                     ALLAWAGWRLLHGPGGAVRIAWLAFALALVALPLLQSLLPGAVAGGAGRTALADDLSA
                     FGIAAPARWSLTPVASREAAFHLLPALAVFAMAMALPAAAHRRLAQLVVALAVASLLL
                     GVAQLGAPQESPLNPYPQWAPAMNGFFANPNHQATLLVVAATLACARLAMVVGAWPPN
                     RPHRVVHGVAAALVMLLAAVALPLTGSRAGVVLIILASAVVVLAHWPAWRGGARSRIV
                     LVACMAVAALALFITLRWMQVDAIDELRGPLRALTGEIAARFAPIGAGVGSYVPVFEQ
                     EAPRDLLMANYVNHAHNEYAQWWLEAGVPAVLVMLLGAIALASTLRMLLRRPAHMRGL
                     GVTALVALGVILAHSFVDYPLRTPAMLAVAAVLAGIAAGQAAQGDEARNREGRRTAAD
                     ATHTEQRGMTT"
     gene            4983..>5772
                     /locus_tag="PLS34_08690"
     CDS             4983..>5772
                     /locus_tag="PLS34_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017055401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRP87583.1"
                     /translation="MTGTSFPTTQHTRIAVVGLGYVGLPLAVEFGKQYDTVGFDINAA
                     RIDELSAGRDSTLEVEPELLAEAARLSFTSDLDGIAACNVYIVTVPTPIDSARRPDLT
                     PLIKASESLGKVLGKGDTVVYESTVYPGCTEEVCVPILERVSGLKFNEDFFVGYSPER
                     INPGDKAHRVTTILKVTSGSTPATASFVDDLYASIINAGTHKASSIKVAEAAKVIENT
                     QRDLNIALVNDLAILFNKLGIDTLEVLEAAGTKWNFLPFRPGLVG"
BASE COUNT          812 a         2171 c         1912 g          867 t
ORIGIN      
        1 catcgaggcg aggccggcga taagcccgct ggtccaatcg ccgaacaggc cgccgaagcc
       61 ggtggcgagc ggccagccgt ctggcgtcgg gatggtggag gcggcgccgg cgagcaggag
      121 aatgccggca aggcaggcgc cggcatccac gctcgaatcc gcgaccgccc ccaccgccgt
      181 ggtggcgccc cggcagacgc caccaccgcc gtggccgacg accgcccctc ccgtccccgc
      241 ggtggccctg gcacatgccg cgacagctgc gccccgacag cccaacccgt tgccgctgga
      301 cgacgcaccg cctgcggctc agccgccgtc ggcgcctgcc gtccccaccg ccaccctcgt
      361 cgccgccagc gcccagcccc ccgcgcctga gcccgccgtg gacgaggatc ccctgctgct
      421 gatgcgccag gccgaagccg cggtgctggc cgtcacccgc tacctcgccg cgcaggacgg
      481 gccgccgccg gcatggcggg atgcgcccac cggccacgcc gccgccgaca tccgccgccg
      541 gctgctcgcg cgcaacgacg gaccggtggc aacgcacctg gcgctgcagt caccgaactg
      601 gacgctcggc acccgcgacg cctcggtgct gggcccttac cgcctcgacg accgccaggg
      661 cctcctggaa accgggttcc tgcacgtcga actggcgcgc tccggccgcc agtggcgggt
      721 ggcggcggtg cgcctggatc cgtcgccgtg agtacgttgc gctggctcct gttcggcgcc
      781 ctgctggcgg ccctggtcgc agccggcgca gcgcggctgg ccacgcacga tgacgcctcc
      841 cgactgcagg ccgacctggc cgccgccgaa gccgccctgc gccaggagga cagcgcccac
      901 gccgtcgcgc tcgcccgcgg cgtgctcgaa cgcgaaccgg tcaacggcca cgctttcggg
      961 gtgctggcgc gggcgctagc cgacgacgga aacgaccagg ccgaccgcct ccgctttaaa
     1021 gcagccgcgc gccgcgcgcc gcgcgatcct ggtgtgcgcg ccaagctggc cgcgtgggcg
     1081 ctggaggccg gcgaccacgc caccgccgtc gaccatctcg acgccctgct gacaggcgcc
     1141 cccggccagc gccagcccat gctccagctc atcgcctcga tgaccccgga ccgcgacttc
     1201 agcgccgccc tggccgcgca cttgatccgg tacccgcagt ggcgcagcgc gatcctgcgc
     1261 gccaccaccg ccagcaagct gcccgannnn nnnnnnggcg cagcgcgatc ctgcacgcaa
     1321 gcgtcgtcca cggctcgggc gagggcgccg accacgtcca cggtgccatg cgcgcccacg
     1381 gtggactgag cgctgcggaa accgcacgct ggatcgacgg catgctccgc gacggccgct
     1441 ggggcagtgc ctacgctcgt tgggtcagcg gcttggacga tgcgccggaa cggctgccgg
     1501 tgccgtggaa cggcgatttc ggacgcgagc cgtcgtcgtc cggattcgac tggcgggtgc
     1561 gccgcacgcc gggcgtgctg ttcgaccggg tggcgctgga cggcggcggc cacgccgcgc
     1621 ggctgcgttt cctggggcgt ccggtggcac gccccgggct cgagatcccg ctgctgctcg
     1681 cccccggcga ccacgtgctg cgcctgcgcg ccggcagcga cggcctgcgc tccgaccagg
     1741 gcctggcctg gtcgctgagc tgcggtgacg gccgcacccg catcgccgag ggcgcgcgcc
     1801 tgcgcgaggc cgccgcctgg acgccgctgg agctggcctt caccgtcccg gacaccgacg
     1861 gctgcgaagg ccagtggctg cgcctgttga acccggcccc ccgcggcgtc ggccagaccc
     1921 tgcgcggtga gatccagttg accgatgtag tgatcgtgcg cgcggccgcc agcccgtgac
     1981 ggcaccgcgg ctccgatacg gtcccgtgac ttggcaagaa acttgctctc tgccactgtt
     2041 cagcatcgaa tccctcttgc catccggacc gcgccatgaa cggaatcacc catctgctcg
     2101 ccgccgccat cctcgcctcg ctgccgctga ccgtggccgc gcaggctgcc gaccccgcgc
     2161 cggccgcgcc gatcgcgctt gagatcgaaa ccggatcggt cctggtcagc accggcggcg
     2221 agttcggtcc cgccgccgcc ggccaaggcc tgcagccggg ccaccgcgtg ctcgtgggtg
     2281 aaggcgcttc ggctgtcctc cgctatcccg acggctgcac cttcgccctg cgcgagcccg
     2341 gggtccaccc ggtggcgagc cagtgccagc ccgcgcgcac gccgtccatg gccccgcgcg
     2401 ggagcacgat cgcgatggtc ggcgtgggtg tcctcgcggt tggtgcgctg gccggtggtg
     2461 gtggcagtga ttccacgccc cagcgaccga tcagtcgcta ggcaacctct gaacaagccg
     2521 ggtttgtcat ttcgagcgca gcgagaaatc tacgctgttg attgccagag atttctctct
     2581 gcgctcgaaa tgacagttgt tcagagattc cccaagccgc catccgctcc ccaacttgca
     2641 catcaacgtc acaagtcatg tgcatcatgt tgactgcgaa gctgaatcga cctgtgtccg
     2701 gtttgcctgc gctctctgtg atccgataaa cttccaaagc agcggtgccc gactgccccg
     2761 ctggaacacg aacccgaagg accaccccca tgcatcagcg tctcctcttt accaccgccc
     2821 tggtctccgc actcgccgcc gctcccgtgc tcgcacagag cagcgccgcc acgctccgca
     2881 tcgacagcgg ttcggcgatg gtgtccaccg gtggcgagtt caccacggcg ggctccggcg
     2941 cccaggtgcc cggcggctcc cggttgatgc tgaccgaagg cagcagcgcc accctggtct
     3001 accccaacgg ctgcaccaag cgcttcgaga gcgccggcgt ccacaacgtc accggcacct
     3061 gctcgctggc cgccagcggc gggtcgggcg caggtggtgc cggcgtggac tggaccggag
     3121 ccggcctcat cgccggtggt accgtcctgg tcggcgcggt cctggcgaac caggacgacg
     3181 tggagatcca ggcgccggtg ccaccggtga gccgctgacc tgatccagcg ccaacgctgt
     3241 tccatgccat gacggcggga cgggtcttac ccgtcccgct ttgttttcag ggcaacgctg
     3301 aaaagtccgg tttgtcattt ctcgatacgc ttgaattgac agttgttcag acgatccttg
     3361 ggaaaggtct gaagcgagta ccgtcatccc cgcgaaggcg gggacccagt gtctttgctt
     3421 tccaggactt gaagacactg ggttcccgcc ttcgcgggaa tgacagcatt tttccagcgt
     3481 agcctcagag atcttctgca ccaagtcgga atgaccaatc cagggttctc acaggttccc
     3541 ttcagggatg cgtagaccat gcacggtgcc gccgcccacc accgcagtca cgattcaccg
     3601 ttgacccccc ttgatcagca ggcccgcgcc gggctcctgg cagtggccat gctgctcgtc
     3661 ctgtgcctgg ccaccggcgg cagctcgcag gagtcgggca gcggtgtctt cgccgcgcaa
     3721 ctgctgtcgc ttgcgctgct ggcctgggcc ggctggcggc tgctgcacgg gcctggcggc
     3781 gcggtccgga tcgcctggct ggcgttcgcg ctggcgctgg tcgcgctgcc cctgctgcag
     3841 tccctgctcc ccggggccgt cgcaggcggc gccggtcgca cggcactggc cgacgacctc
     3901 tcggccttcg gcatcgccgc gcccgcgcgc tggtcgctga cgccggtcgc cagtcgcgag
     3961 gccgccttcc atctgctgcc ggcactggcc gtcttcgcca tggcgatggc cctgccggcc
     4021 gccgcgcatc gccgcctcgc ccagctggtc gtggcgctgg cggtggccag cctgctgctc
     4081 ggcgtggccc agctcggcgc cccgcaggag agcccgctca acccctaccc gcagtgggca
     4141 ccggcgatga acgggttctt cgccaacccc aaccaccagg ccaccttgct ggtggtggcg
     4201 gccacgctgg cctgcgcgcg gctggcgatg gtggtaggcg cctggccccc gaaccgcccg
     4261 caccgagtgg tgcacggtgt cgccgccgcg ctggtgatgc tgctggcggc cgtggccctg
     4321 ccgctgaccg gttcacgtgc gggcgtggtg ctgatcatcc tggccagtgc cgtggtggta
     4381 ctggcgcact ggccggcctg gcgtggcggc gcgcgcagcc gcatcgtgct ggtggcctgc
     4441 atggccgtgg ccgcgctggc cctgttcatt acgctgcgct ggatgcaggt cgacgccatc
     4501 gacgagctgc gcggcccgct gcgcgccctg accggcgaga tcgccgcgcg cttcgccccg
     4561 atcggcgccg gtgtcggcag ctacgtgccc gtgttcgagc aggaggcccc gcgcgacctg
     4621 ctgatggcca actacgtcaa ccacgcccac aacgaatacg cgcagtggtg gctggaggcc
     4681 ggcgtgcccg ccgtgctcgt catgctgctg ggtgcgatcg cgctggcatc gaccttgcgc
     4741 atgctgctgc gccggcccgc gcacatgcgt ggcctgggcg tgaccgcgct ggtcgccctg
     4801 ggcgtgatcc tggcgcactc cttcgtggac tacccgttgc gcaccccggc gatgctggcg
     4861 gtggcggcgg tgctggcggg gattgccgcg ggacaggccg cacagggcga cgaagcgcgt
     4921 aacagggaag ggcgccgcac agccgcggac gctacccaca cagaacagag aggcatgaca
     4981 acgtgacggg cacttctttt cctaccaccc agcacacccg catcgccgtt gtcggcctgg
     5041 gctatgtcgg cctgccgctg gcggtcgagt tcggcaagca gtacgacacc gtcggcttcg
     5101 acatcaacgc cgcgcgcatc gacgaactga gtgccggccg cgacagcacc ctggaagtcg
     5161 agcccgaact gctggccgag gccgcgcgcc tgtcgttcac cagcgacctg gatggcatcg
     5221 ctgcctgcaa cgtctacatc gtcaccgtgc ccaccccgat cgacagcgcc cgccgcccgg
     5281 acctgacccc gctgatcaag gccagcgaat cactcggcaa ggtgctgggc aagggcgaca
     5341 ccgtggtcta cgagagcacc gtctaccccg gatgcaccga ggaagtctgc gtgccgatcc
     5401 tggagcgcgt gagcgggctc aagttcaacg aagacttctt cgtcggctac agccccgagc
     5461 gcatcaaccc cggcgacaag gcgcaccgcg tcaccaccat cctcaaggtg acctcgggct
     5521 cgaccccggc aacggccagc ttcgtggacg acctgtacgc cagcatcatc aacgccggca
     5581 cccacaaggc cagcagcatc aaggtggccg aggccgccaa ggtcatcgag aacacccagc
     5641 gcgacctcaa tatcgccctg gtcaacgacc tcgccatcct gttcaacaag ctcggcatcg
     5701 acaccctgga agtactggaa gccgccggca ccaagtggaa cttcctgccg ttccgcccgg
     5761 gcctggtcgg cg
//
LOCUS       DAOMPG010000150         5723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5944_length_5723_cov_4.277347, whole genome shotgun sequence.
ACCESSION   DAOMPG010000150 DAOMPG010000000
VERSION     DAOMPG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5944_length_5723_cov_4.277347"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLS34_08695"
     CDS             complement(<1..430)
                     /locus_tag="PLS34_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRP87584.1"
                     /translation="MDGPTTTIFGGTGFVGNRIARALVASGGRVRIAARHPRRPDWVT
                     SAHRVELVTADIRNETSVTRAVFGADAVVNAVSCFVESRRHGTFESIHVQGAARLARA
                     AKAAHVQRFVLVSGMGVDPTSPSPYVRARMRGEELTRAAFP"
     gene            complement(514..1584)
                     /locus_tag="PLS34_08700"
     CDS             complement(514..1584)
                     /locus_tag="PLS34_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRP87585.1"
                     /translation="MANSGADRALPTLPRDRFRGCLLGGAVGDALGAPVEFMSHAEIL
                     DRFGPTGITGFAPAYGGLGRITDDTQLTLFTAEGLLEGWASGCTDGRACVVRATHRAY
                     LRWLRTQDENPDACAGDDAPPEGWLLGHRRLHSRRGPGPTCLTALQTAGEPGTPADND
                     RKGCGGVMRVAPVGLFLWRPTMANSYRPAFALASQLAGLTHGHPTGQLAAGVLAVLVQ
                     GLLDDLSLEAALAGGKVILASHPGHEETLSLLEQAEMLARSDLPRAAAVTALGQGWLA
                     EEALAIAVYCALVAESFTDGVVLAVNHDGDSDSTGAIAGHLLGARHGAQAIPSEWLEV
                     LELGDVIADVADRLAQLAGSVG"
     gene            complement(1793..2209)
                     /locus_tag="PLS34_08705"
     CDS             complement(1793..2209)
                     /locus_tag="PLS34_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP87586.1"
                     /translation="MLVQDIMKTKVITVSPLATLREAMAIMSKQNVKSLVVERRDETD
                     AYGLLTYTAILRTIVAEEGDIDLINVYDVCVKPVITVPPKMDLKYVARLMAKHRLGRL
                     VVLEGGDMVGIVTMNDIVGEVLAMADKLHVAEPPEQ"
     gene            complement(2231..2578)
                     /locus_tag="PLS34_08710"
     CDS             complement(2231..2578)
                     /locus_tag="PLS34_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRP87587.1"
                     /translation="MKFAALVLIVAEELEERAIAVAKEAGAGGMTLFNARGISAAEKK
                     TFFGLTYQGSQTMMICILERKLALTVMKALTEGLELKKHSKGVVFTVPLEHIAGMDTR
                     QIESFVEQIREDI"
     gene            complement(2578..4089)
                     /locus_tag="PLS34_08715"
     CDS             complement(2578..4089)
                     /locus_tag="PLS34_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HRP87588.1"
                     /translation="MSQAKEFIVLLGHSFRDLLPIIAVVAIFQFVVIRQLPEDWLPIT
                     AGLLVVVVGVALFLQGLELGIFPVGKNLSNEFALKGSLPLLMIFGFSLGFAAVVAEPA
                     LIAVADQAEIISQGRIKQFTLRMVVACSVGLVVSLGVLRILLGHGLQWYVFIGYLLVT
                     VVTFFAPEEIVGLAYDTGGVTTNIVTVPLIAALGIGLAMSVGGRNAMVHGFGLVAPAV
                     IMPMITVQFLGIIVYNFGEPAEVVAVVAEAVHVDGAPLVEAAAGSLLLGMLTDLLAMF
                     RDVLPIIGVVLFFQYAVIRKPLAHLHRIAGGFVLVIIGLYAFVLGLKMGLFPVGRSMA
                     EQLIQLDGWLFVYLFAFAIGFATTMAEPALIAIGRKAEEAAHGKLDGKVIRLLVAVGV
                     AVGITIGVHRIIAGDSIHHYMIGGFAVVITLTFVAPKYIVGLAYDLGGVTPSAVTVPL
                     VTALGLGLATYIEGRNVLIDGFGLIAFVSLFPIISVMVYAILVEFAKRRRGSA"
     gene            complement(4221..4781)
                     /locus_tag="PLS34_08720"
     CDS             complement(4221..4781)
                     /locus_tag="PLS34_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRP87589.1"
                     /translation="MQFRMILALLLGAALCWQPAQADGRWTASLHLGNAAVDRLVEDD
                     GQWWSQVDDNGAAVGVSVTYEHSPIFGLRLMYELTAELEAGNRCPPGEVCPAVAIDEE
                     VDFSAWQVAAVPRLPLGPDWSLFGTLGAMRWKLHQDDILPGDSGTAFVYGLGLAWRAE
                     RIELGVEYQRADIDLDTFRLSLGLRF"
     gene            complement(4795..5148)
                     /locus_tag="PLS34_08725"
     CDS             complement(4795..5148)
                     /locus_tag="PLS34_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRP87590.1"
                     /translation="MSDDRLEDLRRRLAAFAAARDWDQFHSPKNLAMALAAEAGELLE
                     HFQWLSESQSETLDAVRRDAVALELADVLLYLVRLADKLDIDLVAAAHRKIGLNELKY
                     PSDKARGNARKYDQL"
     gene            complement(5291..>5723)
                     /locus_tag="PLS34_08730"
     CDS             complement(5291..>5723)
                     /locus_tag="PLS34_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRP87591.1"
                     /translation="GVLRDAETRRSFMANALGTATFLLRWLALAFVLESLMVAWLPAD
                     RVLALLGDEAMAIPMAVLVGVPAYFNGFAALPLVAGLVDLGMSPAVALAFLVAGGIAS
                     IPAAAAVWALAQPRVFVLYLGLGATGSLLAAYTYSLAKGVM"
BASE COUNT          975 a         1945 c         1972 g          831 t
ORIGIN      
        1 cgggaaaggc ggcgcgcgtc agctcctcgc cgcgcatccg ggcgcgcacg tagggtgagg
       61 gcgaagtcgg gtcgacaccc atccccgaga ccaggacgaa acgctgcacg tgggcggcct
      121 tcgcggcgcg ggccagccgc gcggcgcctt gcacgtggat cgactcgaag gtgccgtggc
      181 ggcgcgactc gacgaagcag ctgacggcgt tgaccaccgc gtcggccccg aacaccgccc
      241 gggtcacgct ggtctcgttg cggatgtcgg cggtcacgag ctcgacccgg tgcgcgctgg
      301 tcacccagtc cggccgccgc gggtgccggg cggcgatgcg gacccggccg ccggaggcga
      361 ccagggcacg ggcgatgcga ttgccgacga agccggtgcc gccgaaaatc gtggtggttg
      421 gaccgtccat ggcagttcct tggggggaga cggctcaggc gaagactaac cccatccggg
      481 gcgaattggc agcgtgaccg accggcggcc gcctcacccg accgaccccg ccagctgcgc
      541 cagccggtcg gcgacgtccg cgatcacgtc gccgagctcc agtacttcga gccattccga
      601 cgggatggcc tgggcgccgt gccgggcgcc gagcaggtgc ccggcaatgg cgccggtgga
      661 gtccgagtcg ccgtcgtggt tgacggccag cacgacgccg tcggtgaagg actcggccac
      721 cagcgcgcag tacacggcga tcgccagcgc ttcctcggcg agccagcctt gcccgagcgc
      781 ggtgaccgcg gcggcgcgcg gcaggtcgga gcgcgccagc atctcggcct gctcgagcag
      841 cgacagggtc tcctcgtggc cgggatgact ggcgagtatt accttgccgc cggccagcgc
      901 cgcctccagc gacaggtcgt ccagcaggcc ctgcaccagc acggcgagga cgccggcggc
      961 cagctgcccg gtggggtggc cgtgggtcag gccggccagc tggctggcca gcgcaaaggc
     1021 cgggcgatag gagttggcca tcgtcggccg ccacaggaac agccccacgg gcgcgacgcg
     1081 catgaccccg ccgcagccct tgcggtcgtt gtccgccggc gtgccgggtt cgcccgcggt
     1141 ctgcagcgcc gtcaggcacg tgggtccggg tccgcggcgg ctgtgcagcc ggcggtggcc
     1201 cagcagccag ccttccggtg gagcgtcgtc gccggcgcaa gcgtccgggt tctcgtcctg
     1261 ggtgcgcagc cagcgcaggt aggcgcggtg ggtcgcgcgc accacgcagg cgcggccgtc
     1321 ggtacagccg gacgcccagc cctccagaag accttcggcg gtgaacagcg tcagctgtgt
     1381 gtcgtcggtg atccggccga ggccgccgta ggcgggggcg aagccggtga tgccggtggg
     1441 cccgaagcgg tcgaggatct cggcgtggga catgaattcg accggtgcgc cgagcgcgtc
     1501 gccgacggcg ccgccaagca ggcagccgcg aaagcggtcc cttggtaacg tggggagcgc
     1561 acggtccgca cccgaattcg ccatgacggc actccttgcc agcccggggg tccgatgatg
     1621 cgcggagccg ggctcagggc gtgagccagc gcggcacgcg ccgtcggcaa ggccaggcac
     1681 cggccgcgct tccttatacc gaatatggcg ccatcggacc agaaacggaa ttcgtagaag
     1741 gcgcagtcgg cgcctcgggc gggtttgtgc gcgccggcct ggccggcgcc ggctactgtt
     1801 ccggcggctc cgcgacgtgc agcttgtcgg ccatcgccag cacctcgccg acgatgtcgt
     1861 tcatggtgac gatgccgacc atgtcgccgc cctcgagcac caccagccgg cccagtcggt
     1921 gcttggccat gagccgcgcg acgtatttga ggtccatttt cggcggcacc gtgattaccg
     1981 gcttgacgca cacgtcgtag acattgatga ggtcgatgtc gccttcttcc gcgacgatgg
     2041 tgcgcaggat cgcggtgtag gtcagcaggc cgtaggcgtc ggtctcgtcg cggcgctcca
     2101 cgaccagcga cttgacgttc tgcttgctca tgatggccat cgcctcgcgc agcgtcgcca
     2161 gcggcgagac ggtgatcacc ttggtcttca tgatgtcctg tacgagcatg gtgcctccgg
     2221 tggaatccgc tcagatgtct tcgcgtatct gttcgacgaa actctcgatc tgccgggtgt
     2281 ccatgccggc gatgtgttcc agcggcacgg tgaacaccac gcccttggag tgcttcttca
     2341 gttccaggcc ctcggtgagc gctttcatta cggtcagggc gagcttgcgc tccaggatgc
     2401 agatcatcat ggtctgcgag ccctggtagg tcaggccgaa aaaggtcttc ttctcggcgg
     2461 cgctgatgcc gcgcgcgttg aagagggtca tgccgccggc gccggcttcc ttggccaccg
     2521 cgatggcccg ctcttccagt tcttcggcga cgatcaggac cagtgctgcg aacttcatca
     2581 ggcgcttcct ctccgcctct tggcgaactc gacgaggatg gcgtaaacca tcacggatat
     2641 tatcggaaac agggacacga acgcgatcag cccgaagccg tcgatcagca cgttgcgtcc
     2701 ctcgatatag gtggcgagcc cgagccccag tgcggtgacc agcggcacgg tcacggccga
     2761 aggcgtcacg ccgccgagat cataggccag ccccacgatg tacttcgggg cgacaaaagt
     2821 cagcgtgatc acgacggcaa agccgccgat catgtaatgg tgaatcgagt cgccggcgat
     2881 gatccggtgc acgccgatgg tgatgcccac ggcgacaccg acggcgacca gcaggcggat
     2941 caccttgccg tcgagcttgc catgtgccgc ttcctcggct ttgcggccga tggcaatcaa
     3001 tgccggctcg gccatggtcg tggcgaagcc gatggcgaag gcgaacaggt agacgaacag
     3061 ccagccgtcg agctggatga gctgctccgc catgctgcgc ccgacgggaa acaggcccat
     3121 tttcagcccg agcacgaagg cgtacaggcc gatgatcacc agcacgaatc cgccggcgat
     3181 gcggtgcagg tgggccagcg gcttgcgaat cacggcgtac tggaagaaca gcacgacgcc
     3241 gatgatcggc agcacgtccc ggaacattgc cagcaggtcg gtcagcatgc cgagcagcag
     3301 gctgccggct gccgcctcga ccagcggcgc gccatcgacg tgcacggcct cggcgaccac
     3361 ggcgacgacc tctgcaggct cgccgaagtt gtagacgatg atgcccagga actggaccgt
     3421 aatcatcggc atgatcacgg ccggcgccac cagcccgaag ccgtgtacca tcgcgttgcg
     3481 gccccctacc gacatggcca gcccgatgcc gagggcggcg atcagcggca cggtgacgat
     3541 gttggtggtt acgccgccgg tgtcgtaggc gagaccgacg atctcctcgg gcgcgaagaa
     3601 cgtgacgacg gtgaccagca ggtagccgat gaacacgtac cactgcaggc cgtgaccgag
     3661 caggatgcgc agcaccccga gcgataccac cagtccgacg gagcacgcga ccaccatgcg
     3721 cagggtgaac tgcttgatgc ggccctggct gatgatctcg gcctggtcgg ccacggcgat
     3781 cagcgccggc tccgccacga ccgcggcaaa gcccagcgag aagccgaaga tcatcagcag
     3841 cggcagcgat cccttcaggg caaactcgtt ggagagattc ttgccgacgg ggaagatgcc
     3901 gagctcgagg ccctgcagga acagtgcgac gccgacgacc accacgagga ggcccgcagt
     3961 aatgggcagc cagtcttccg gcagctggcg gatgacgacg aactggaaga tggcgaccac
     4021 agcgatgatc ggcagcaggt cacggaagga gtgaccgagc aacacgatga attccttcgc
     4081 ctggctcaca ctccccccgg gtcggaccct gacaagcgca tgatattacg gaaaaaacca
     4141 gcaaaaaggg gccgtcgcgg ccccttggga gaacgatttt tggccgccag cggccgtcta
     4201 aggcgcgctt gcaggtgccg tcagaagcgc aggcccaggc tcagccggaa ggtgtcgaga
     4261 tcgatgtcgg cccgctggta ctcgacgccg agctcgatgc gctcggcccg ccacgccagc
     4321 cccaggccgt agacgaaggc ggtgccggag tcgccgggca ggatgtcgtc ctggtgcagc
     4381 ttccagcgca tcgcgccgag cgtgccgaac agcgaccagt ccgggccgag cggcaggcgc
     4441 ggcacggccg caacctgcca tgccgagaaa tccacttctt cgtcgatggc cacggccggg
     4501 caaacctcgc cgggtggaca gcggttaccg gcctcgagct ccgcggtgag ttcgtacatt
     4561 agccgcagcc cgaaaatcgg cgagtgctcg taggtcacgc tgacgccgac ggccgcgccg
     4621 ttgtcgtcca cctggctcca ccactggccg tcgtcctcca ccagccggtc gacggcggca
     4681 ttgcccaggt gcagcgacgc ggtccatcgg ccgtccgcct gtgcgggttg ccagcacagg
     4741 gccgcgccca gcaacagcgc cagtatcatc cggaattgca tcacgacagg cctcctacag
     4801 ctggtcgtac ttgcgcgcat tgccgcgcgc cttgtccgac gggtacttga gctcgttcag
     4861 cccgatcttg cgatgcgccg cggcgaccag gtcgatgtcg agcttgtccg ccaggcgcac
     4921 caggtagagc agcacgtcgg cgagctccag cgcaacggcg tcacgccgga ccgcatccag
     4981 cgtctcgctt tgcgactcgg acagccactg gaagtgctcc agcagctcgc cggcttccgc
     5041 cgccagcgcc atggccaggt tcttcggcga atgaaactgg tcccagtcgc gtgccgcggc
     5101 gaacgcggcg agacgccggc gcaggtcttc gaggcgatca tcgctcatgt tcagcagtgt
     5161 agctgcccgc cgccgccgct gtaacgtcgc ccggacaagg gcgcggcccc gccacgcagg
     5221 ccggcggggc cgcgccgcca cgatcctcag cagcagccgg gaccgcagca accgtcgtcg
     5281 atggtctgca tcacatcact cctttcgcca ggctgtaggt gtaagcggcc agcagcgagc
     5341 ctgtggcgcc gaggccgagg tagagcacga atacacgggg ctgcgccagc gcccagaccg
     5401 cggcggctgc cgggatgctg gcgatgccgc cggcgaccag gaaggccagc gcgaccgccg
     5461 ggctcatgcc caggtcgacg agcccggcga ccagcggcag tgccgcgaag ccgttgaagt
     5521 aggcggggac cccgaccagc acggccatcg ggatggccat ggcctcgtcg ccgagcagcg
     5581 ccagcacccg gtccgccggc agccacgcga ccatcaggct ctcgagcacg aaggcaagcg
     5641 ccagccagcg caacaggaac gtggcggtcc cgagggcatt ggccatgaag ctgcggcggg
     5701 tctctgcatc ccgcagcacg ccc
//
LOCUS       DAOMPG010000151         5717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_5956_length_5717_cov_4.054928, whole genome shotgun sequence.
ACCESSION   DAOMPG010000151 DAOMPG010000000
VERSION     DAOMPG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5956_length_5717_cov_4.054928"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="waaA"
                     /locus_tag="PLS34_08735"
     CDS             complement(<1..1002)
                     /gene="waaA"
                     /locus_tag="PLS34_08735"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HRP87592.1"
                     /translation="MHRIYLVISYLAAPLVALLLFWKGLGNRAYWERFEERFGFGRSR
                     LESPGIWVHAVSVGEVVAASSLIAALRRDYPQLPIVVTTVTPTGAQRVRDLFGDEVLH
                     SYAPYDTIGSVRRFFERMQPRLAIVMETELWPNMYAACGARGVPLVLANARISPRSVA
                     RYRRFIGLFRQALANGVVIAAQSDQDAERFLSLGADPARTVVTGNLKADLEFPPGMTA
                     AGRQFRTDHIGARPTWIAASTHAGEEEAALDAHARVRTRHRDALLLLVPRHPDRFDGV
                     AELLNTHGITFARRSRDETPVPEHAVYLVDSLGELPMFYAAADVAFVGGSLVPVGGHN
                     "
     gene            1118..2077
                     /gene="lpxL"
                     /locus_tag="PLS34_08740"
     CDS             1118..2077
                     /gene="lpxL"
                     /locus_tag="PLS34_08740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005743829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HRP87593.1"
                     /translation="MSTGQTPLYRFLAPRYWPIWLALGLVRLLVALPFRAQLAAGRLI
                     GRLGYRIAPERRAIAARNLELAFPELDAAARHELLLRHFDALGMSVIETGMCWWADDR
                     RIGRLAEVRGLEHVEAALAAGRGAIMLTGHFTTLDLGGRFLTRRAPVAAMYRPHRNPL
                     FEEIMRRGRERAARQAFPKRDVRAMIRGLRTNHLIWYAPDQAHAHGKYSAVVPFFGVP
                     APTNTATSAFARLGNAPVIPFFPVRLPGKAGYRLDILPPLPDFPSDDPAADAARINRL
                     LEERIRLCPEQYLWVHRRFKPAGGDGTDYYGPLARRRKSPPAS"
     gene            complement(2032..2766)
                     /locus_tag="PLS34_08745"
     CDS             complement(2032..2766)
                     /locus_tag="PLS34_08745"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HRP87594.1"
                     /translation="MSAAIARGPGWAILYDPALVSGPGPEWFDPGHWDGRVAGRADRG
                     RGNLLFVDAGPRQWALRHYRRGGMAGRLLVDRFVWCGESRTRSFREWRMLARLREAGM
                     PVPRPVAAHWRRRGLAYVADLATERIPGAVPLSVHIAAGEAVDWGAVGGVIRAFHDLG
                     AWHADLNAHNILLDPAGGIWLLDFDRGSFRAPGAWQRKSLDRLNRSLRKIAAEEAGPG
                     FDSTGWSGLLQGYEAGGDLRRRASGP"
     gene            complement(2777..2986)
                     /locus_tag="PLS34_08750"
     CDS             complement(2777..2986)
                     /locus_tag="PLS34_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRP87595.1"
                     /translation="MSEPGTIEANTQRHYEVTRADLPLSCPTPAMKLWNSHPRVYLPI
                     EATGRAQCPYCSAVFTLVDNATVAP"
     gene            complement(3026..>5717)
                     /gene="glnE"
                     /locus_tag="PLS34_08755"
     CDS             complement(3026..>5717)
                     /gene="glnE"
                     /locus_tag="PLS34_08755"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HRP87596.1"
                     /translation="RGAEELPGLAVAVQRRWTPGELLAATPGLLEEANDEAAVMRRLR
                     LFRRREMLRIAWRDLAGWAVLEETLGHLSELAEVCLRAADDWVHRDMVARHGEPRSVD
                     GVAQRLLVVAMGKLGGGELNFSSDIDLVFLYPESGETDGERCLSNEQFFTRAGRQLIR
                     LLDEGTQDGFVFRVDMRLRPFGESGPLAMSLAAFANYLQQHGRTWERYAYVKARAVTG
                     HHVGMGLYRDVLRPFVFRRYLDFGLFDALRDMKSKISLEVQRRELRGNIKLGRGGIRE
                     IEFIVQCLQLLRGGADPRLREPGLPAALSRLAETGQLPAGAVTELREAWRFLRRTENR
                     LQAWQDRQTHELPADEPGRLRLAFAMGFDAWPAFEAELAAHQQRVDAWFRRQTADDRA
                     PEPEDGVAAAWRADGESAAATASLSAAGFDAPERALAAMRSLAEASWYRRLDETARQR
                     LATLVPALARGAGELPAPEEALERLLRVVSAIGGRSAYYALLNENPAARARFIKLCGS
                     SEFLASQVAVHPLLLDDLIDPRVMETPPGRDELAAELDERLAGVEPEDLETVMDALRN
                     FQRAAVFRTAVADLTGRLPLMKVSDQLTWIAEVLLEACIRIARDDAQQRHGRPWCGDR
                     EDALRECNMAIIGYGKLGGLELGYGSDLDLVFVHDASGEIQRTEGPAVIEAGTLFSRM
                     TRRIVHLLATPTGAGVLYEVDTRLRPSGKGGLLVTSLGAFESYQREAAWTWEHQALLR
                     ARAVAGDPGVRRAFEQLRVRVLVEAVSRDGLRTEVATMRERMRNELSRGRQDTFDLKQ
                     DAGGIADIEFLVQYLVLQNAARSPELLRWPDNMRQIEDLRDAGLLAREDSELLHQAYL
                     DYRQKLHRLALANSPGLVPVADVAVLSDRVRALWQRIMEP"
BASE COUNT          858 a         2139 c         1928 g          792 t
ORIGIN      
        1 gttgtggccg ccgaccggca ccaggctgcc gccgacgaac gccacatctg cggccgcgta
       61 gaacatcggc aattcgccaa ggctgtccac caggtagaca gcgtgttccg gcacgggcgt
      121 ttcgtcgcgc gagcgacgtg cgaacgtgat gccgtgcgtg ttcagcagct ccgcgacgcc
      181 gtcgaagcga tccggatgcc tgggcaccag cagcagcagc gcgtcgcgat gccgcgtgcg
      241 cacccgcgcg tgggcgtcca acgcggcttc ctcctcgccc gcgtgagtgc tggccgcaat
      301 ccaggtcggc cgggcgccga tgtgatccgt cctgaattgc cggcccgccg cagtcatccc
      361 cggcgggaat tcgaggtcag ccttcaggtt ccccgtgacc accgtgcggg cgggatcggc
      421 gcccagcgag agaaaccgct cggcatcctg gtcgctctgt gcggcgatca cgacgccgtt
      481 ggcgagcgcc tgccggaaca ggccgatgaa gcgccggtaa cgcgccaccg agcgcggcga
      541 tatccgcgcg ttcgccagca ccagcggcac gccccgcgca ccgcaggcgg cgtacatgtt
      601 cggccacagc tcggtctcca tgacgatcgc caggcggggc tgcatgcgct cgaagaagcg
      661 gcgcacggac ccgatggtgt cgtacggggc atagctgtgc agcacctcgt cgccgaacag
      721 gtcgcgcacg cgctgcgcgc cggtcggggt gaccgtggtg acgacgatcg gcagctgcgg
      781 atagtcgcgt cgcagcgcgg cgatcaggga tgaggcagcg accacctcgc ccaccgacac
      841 cgcgtgtacc cagatgccgg gtgattccag tcggctgcgg ccgaagccga accgctcctc
      901 gaagcgctcc cagtaggcgc ggttgccaag ccctttccag aacagcagca aagcgacgag
      961 cggggccgcg agataggaga tgacgaggta gatgcggtgc aaagcggatc gcccggcggg
     1021 gtggcggcga tggtagcaca ccgctccggg caggccccgg cggcatcgct ataatgcagg
     1081 ccacgcgcgc atgcgcggct cggaaaccgg caccaacttg agcaccggac agacgcctct
     1141 ctaccgcttc ctggcgccgc gctactggcc catctggctg gcgctgggac tggtgcgcct
     1201 gctggtcgcg ctgccttttc gcgcacagct cgcggccggc cggctgatcg gccggctcgg
     1261 ctaccgcatc gccccggagc gccgcgcgat cgccgcccgc aacctcgagc tggcattccc
     1321 ggaactggat gccgcggccc gccatgaact gctgctgcga cacttcgatg ccctcggcat
     1381 gtcggtcatc gagaccggca tgtgctggtg ggccgacgac cggcgcatcg ggcggctggc
     1441 cgaggtccgc ggactggagc acgtcgaggc cgcgctggcc gcgggccgcg gcgccatcat
     1501 gctgaccggg catttcacga cgctggacct ggggggccgc ttcctcacgc ggcgagcgcc
     1561 cgtggctgcc atgtaccgtc cgcatcgcaa cccgctgttc gaggagatca tgcggcgcgg
     1621 gcgcgaacgc gccgcgcggc aggccttccc gaagcgggac gtgcgggcga tgatccgcgg
     1681 cctgcgcacg aaccacctga tctggtatgc gccggaccag gcgcacgcac acggcaagta
     1741 cagtgcggtg gtgcccttct tcggcgtgcc ggcgccgacc aacaccgcaa cctcggcctt
     1801 cgccaggctc ggcaacgcgc cggtgattcc gttcttcccg gtccgcctgc ccggcaaggc
     1861 gggctaccgg ctggatatcc tgccgccgct gccggacttc ccgagcgacg acccggccgc
     1921 cgacgccgcg cgcatcaacc ggctgctcga ggaacgcatc cgcctctgcc ccgagcaata
     1981 cctgtgggtg caccgccgct tcaagccggc cggcggcgac ggcacggatt actacgggcc
     2041 gctggcgcgc cgccgcaagt ccccgcccgc ttcgtaacct tgcagcaacc cggaccagcc
     2101 ggtggaatcg aaacccggtc ccgcttcctc cgccgcgatc ttgcgcagcg agcgattcag
     2161 gcggtcgaga ctcttgcgct gccaggcgcc cggcgcgcgg aagctccccc ggtcgaagtc
     2221 cagcagccag atgccgccgg ccgggtcgag caggatgttg tgggcgttca gatcggcgtg
     2281 ccaggcgccc aggtcgtgga atgcccggat cactccgccg acggcacccc agtcgacggc
     2341 ttcgccggcg gcaatgtgca ccgacagcgg cacggcaccc gggatgcgct ccgtcgccag
     2401 gtcggccaca taggccaggc cgcgacgccg ccagtgggcg gccaccggcc ggggcaccgg
     2461 catgccggcc tcacgcaggc gggcgagcat gcgccattcg cgaaaggaac gcgtgcgcga
     2521 ctcgccgcac cacacgaagc gatcgaccag caggcggccg gccatgccgc cgcggcggta
     2581 atggcgcagc gcccattgtc gcggcccggc gtcgacgaac aggagattgc cgcggccgcg
     2641 gtccgcacgg cccgccacac gcccgtccca gtgcccggga tcgaaccact ccgggcccgg
     2701 accggagacc agcgccgggt cgtacaggat cgcccagccc ggcccgcgcg cgatcgccgc
     2761 gctcaccgaa ttcgcctcag ggcgcgacag tcgcgttgtc caccagcgta aagaccgcac
     2821 tgcagtacgg gcactgcgca cggccggtcg cctcgatggg caggtagacc ctggggtggg
     2881 agttccacag cttcatcgcc ggcgtcgggc acgacaaggg caggtcggcg cgggtcactt
     2941 cgtagtgccg ctgcgtgttc gcctcgatgg tgccgggctc ggacatgcgc ctcctccctc
     3001 gccgaagcgc gcattatagc cgcgttcagg gctccatgat gcgctgccag agagcgcgca
     3061 cccgatcgct caggaccgcc acgtcggcca ccggcacgag cccgggtgaa ttggcaagcg
     3121 ccaggcgatg cagcttctgg cgatagtcca ggtaagcctg gtgcagcagc tcgctgtctt
     3181 ccctggccag caggcccgcg tcacgcagat cctcgatctg gcgcatgttg tccggccagc
     3241 gcagcagctc ggggctgcgc gcggcattct gcagcaccag gtactgcacc aggaactcga
     3301 tgtccgcgat cccgcccgcg tcctgcttga ggtcgaaagt atcctgcctg cccctggaga
     3361 gctcgttgcg catgcgctcg cgcattgtcg cgacctccgt gcgcaggccg tcgcggctca
     3421 ccgcctcgac cagcacccgc acccgcaact gttcgaaggc ccggcgcact ccggggtcgc
     3481 ccgccacggc ccgcgcccgc aacagcgcct ggtgctccca ggtccacgcc gcctcccgct
     3541 ggtagctctc gaaagcgccc aggctcgtca ccagcagccc gcccttgccg ctcggccgca
     3601 agcgcgtgtc cacctcgtac agcacgccgg cgccggtcgg cgtcgccagc aagtgcacga
     3661 tgcgacgggt catgcgcgaa aacaacgtgc ccgcctcgat cacggccgga ccctcggtgc
     3721 gctggatctc gccgctggcg tcgtgcacga acaccaggtc cagatccgag ccgtagccga
     3781 gctccagccc gcccagcttg ccgtagccga tgatcgccat gttgcattcg cgcagcgcgt
     3841 cctcgcggtc gccgcaccag ggccggccgt ggcgctgctg cgcgtcgtcg cgcgcgatcc
     3901 tgatgcaggc ctccagcaac acctctgcga tccacgtgag ctggtcgctc accttcatca
     3961 acggcagccg ccccgtcagg tccgccacgg ccgtgcggaa cacggcggcc cgctggaagt
     4021 tgcgcagtgc atccatcacg gtctcgaggt cctccggttc gacgccggcg agccgctcgt
     4081 ccagttccgc ggccagctcg tcacgccccg gcggcgtctc catcacgcgg ggatcgatca
     4141 ggtcgtcgag cagcaaggga tgcaccgcca cctggctggc caggaactcg ctgctgccgc
     4201 aaagcttgat gaagcgcgcg cgggccgcag gattttcgtt cagcagcgcg tagtaggcgc
     4261 tgcggccgcc gatggcactg acgacgcgca acagccgctc gagcgcctcc tccggcgccg
     4321 gcagttcccc tgcgccgcgc gccagcgccg gcaccagcgt cgcgagacgt tgccgcgcgg
     4381 tctcgtcgag acgtcggtac cagctcgcct cggccaggct tcgcatcgcc gcgagggcgc
     4441 gctccggcgc gtcgaagccg gccgccgaca ggctcgccgt cgccgccgcc gactccccgt
     4501 cggcgcgcca ggccgccgcc acgccgtcct cgggctccgg cgcgcgatcg tcggccgtct
     4561 ggcgcctgaa ccaggcgtcc acccgctgct ggtgcgcggc cagctcggcc tcgaatgccg
     4621 gccaggcatc gaagcccatg gcgaaggcca gccgcaggcg gcctggctcg tcggccggca
     4681 actcatgggt ctggcgatcc tgccaggcct gcagccggtt ctccgtgcgg cgcaggaagc
     4741 gccaggcctc gcgcagttcc gtgaccgccc cggccggcag ctggccggtc tccgccagcc
     4801 gcgacagcgc cgccggcaga cccggctccc gcaaacgcgg atcggcgccg ccgcgcagca
     4861 gttgcaggca ttgcacgatg aactcgatct cgcgaatgcc gccgcggccg agcttgatgt
     4921 tgccgcgcag ctcgcgccgc tggacctcca gcgagatctt gctcttcatg tccctgagcg
     4981 cgtcgaacag accgaagtcg agatagcggc ggaatacgaa gggacgcaac acgtcgcggt
     5041 agaggcccat gccgacatgg tggccggtca ccgcccgcgc cttcacatag gcgtagcgtt
     5101 cccaggtgcg gccgtgctgc tgcaggtagt tggcgaacgc cgccaggctc atggccagcg
     5161 gaccgctctc gccgaacggc cgcaggcgca tgtccacgcg gaacacgaag ccgtcctgcg
     5221 taccctcgtc cagcaggcgg atcagctggc gaccggcgcg cgtgaagaac tgctcgttgc
     5281 tcaggcagcg ctcgccgtcg gtctcgccgg actccggata caggaacacc aggtcgatgt
     5341 ccgaagagaa gttcaactca ccgccgccga gcttgcccat ggccaccacc agcaggcgct
     5401 gggccacgcc gtccaccgag cgcggctcgc cgtggcgggc gaccatgtcg cgatggaccc
     5461 agtcgtcggc ggcccgcagg cagacctcgg cgagttcgct gaggtggccg agggtctcct
     5521 ccagcaccgc ccagccggcg agatcacgcc aggcgatgcg cagcatctcg cgccgcctga
     5581 acagtcgcag gcgacgcatc accgccgctt cgtcgtttgc ctcctcgagc agccccgggg
     5641 tggcggccaa cagttctccc ggcgtccagc ggcgctgcac ggccacggcg agcccgggca
     5701 gctcctcggc gccgcgc
//
LOCUS       DAOMPG010000152         5622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6076_length_5622_cov_4.263517, whole genome shotgun sequence.
ACCESSION   DAOMPG010000152 DAOMPG010000000
VERSION     DAOMPG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_5622_cov_4.263517"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLS34_08760"
     CDS             <1..300
                     /locus_tag="PLS34_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87597.1"
                     /translation="PRLVMGPAGDGGFWLLGANLDPAPADWTRAPCGRADTATGFREA
                     MGRHGQWRTLPVLTDVDEAADLAPMLAEMDRLDHPTPAQSRLARWSRAALAAQRA"
     gene            393..647
                     /locus_tag="PLS34_08765"
     CDS             393..647
                     /locus_tag="PLS34_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87598.1"
                     /translation="MAKNPRKAGLDIVVKVTDSDDNLRGRFLISAGSLYYFRKNAREP
                     SRRYSWRQLIEMIEDQSAAKGTAAKSAAKPAAKTPSTRKR"
     gene            complement(682..2052)
                     /locus_tag="PLS34_08770"
     CDS             complement(682..2052)
                     /locus_tag="PLS34_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP87599.1"
                     /translation="MAKDSLRRRVLLSLGIALAVFFGATVVVLEWSLATSLERSRDEV
                     LQAQLMALIAMAEPTEPADAPPGLTLPEPPDGRMLSPGSGLLAEIRDARGAAVWRSPS
                     ALGVELEVPPAGAPGAYARWVQAESDGPGLALAGITIDWEFEDESVASFRFIVGQSLE
                     PWRAQRRAFRLGLVGWFGALAAAMLFVLAVVLRRVLQPLGRIEREIHEMETAQRDQLS
                     AAWPEELRGVAANLNALLRRERARQQRYQESLANLAHSLKTPLAAVRTLLPEVDDGRS
                     DRVRVIRTELSRMDQIIGWQLRRAGAAGAAGGVGLVPVAVAPVARDLAATLDKVYAGR
                     GSSCELDIPATVRFRGDEGDLQEILGNLLDNAWKYGRRRIRCSAAVISPGAIELRVED
                     DGAGLDPERASAAMARGARLDESHPGQGIGLAVVNELVSAYGGGIEFERSSLGGTAVV
                     VRLPGA"
     gene            complement(2060..2746)
                     /locus_tag="PLS34_08775"
     CDS             complement(2060..2746)
                     /locus_tag="PLS34_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP87600.1"
                     /translation="MRALLVEDDEALRHGLAAAMRAAGWQVDEAGDGREGLYLAEEST
                     PDVAVVDLGLPGMSGMDLISALRKNRRELPVLILTARDRWQDKVAGLKAGADDYMVKP
                     FHTEELLARLEALVRRAAGWAQPVLDFGVLRFEPAAQRVELHGEPVELTGFEYRLLEY
                     LVLNAGKVVSRSELIDRLYDQDYDRDSNTIEVFVGRLRRKLDPDGGLKPIETLRGRGY
                     RFALTPAGKD"
     gene            complement(2795..3115)
                     /locus_tag="PLS34_08780"
     CDS             complement(2795..3115)
                     /locus_tag="PLS34_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRP87601.1"
                     /translation="MPNPPLQRRRHLRRGASAALLCAALLFAGATAPTTPALAQALQV
                     AQANNGISLAEATRMVSERTGGQVLRAETKRDKGRAVHRIRVLTEDGRVRTWHVDAET
                     GRIY"
     gene            complement(3218..3808)
                     /locus_tag="PLS34_08785"
     CDS             complement(3218..3808)
                     /locus_tag="PLS34_08785"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRP87602.1"
                     /translation="MNNKLMLKLAIAGLLVSVAGTAAADPRGKSGVDHARVVDVTPIV
                     RQVRVETPVRECWQETEYQTIETRSYGPGRSGHASPLPTIAGGIIGGVVGSQFGGGSG
                     KAAMTAVGALVGASMGAQAEERRVYAGGPGRVAYEQRPVTVQHCQTTTSYQLQEHVEG
                     YRVTYVYGGREYQTTMASRPGRTIPVRVTVVPSGRF"
     gene            4119..>5622
                     /locus_tag="PLS34_08790"
     CDS             4119..>5622
                     /locus_tag="PLS34_08790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP87603.1"
                     /translation="MTPEIREALQYCPNLPSPPGIAIRIVELGRDPNVDLTALSRLLA
                     RDPALASRVLRASNSALYAQRRRSDNLRQALVVIGLNATMTLALSFSLAETITDQPAT
                     RNVNRLVWRRALIAATAARLLGTRLKLQELEELFLAGLLQDIGILALDAAIPESYHPL
                     VAEARDHDDLLARERAALEADHGDAGEWLMTNWGLPKRLARVASAVHAPEQARLSGSE
                     ALFLNCVAVAGKIADLFLGEDRKAGTDRVADDARHLLGIRQEDIEPLLNEVAEVLPEV
                     AALYDTEIISARKAAGVIDQAREILAARNLHLIQEVAEQTRKVQEMERTTQHLRDSAH
                     RDALTGLLNRGRFDEVLESEFMMATESGWPLTIGFIDLDHFKSVNDTHGHQVGDAVLS
                     RVASILASHLRERDFIMRYGGEEFVALLPGTGIEQAQGVFERLRKAVADEIHKGEAGA
                     RFQTTVSIGLATHMDGSRRAASAVDLVRSADRALYEAKRTGRNRILVDKTI"
BASE COUNT          857 a         2024 c         1946 g          795 t
ORIGIN      
        1 ccgcggctgg tcatgggccc ggccggcgat ggcggcttct ggctgctggg cgcgaacctc
       61 gatcccgcgc cggcggactg gacacgcgct ccgtgcggcc gcgcagacac agcgacgggc
      121 ttcagggagg ccatgggacg gcacggtcag tggcggacgc tgccggtgct gacggacgtc
      181 gacgaggcgg cggatctcgc gcccatgctg gccgaaatgg accggctcga ccatcccacg
      241 cctgcgcagt cccggctggc ccggtggagc cgcgccgcac tggcggcgca aagagcgtag
      301 caagtgcttg cgcgcgacgc atggggtaca tttgagggct gtggcgccgc ttttccggcg
      361 gccgggacaa gttcaagaac aaggagcacc ggatggcgaa gaatccgcgc aaggccggac
      421 tggacatcgt ggtcaaggtc acggacagcg acgacaacct gcggggccga ttcctgatct
      481 cggcgggcag cctgtactac ttccgcaaga acgccaggga accctcgcgc cgctactcat
      541 ggcgccagct catcgagatg atcgaagacc agtctgcggc gaagggcacg gccgcaaagt
      601 ctgccgcgaa acccgccgcg aagacgccca gtacccgcaa gcgctgagcc cgcgcacccg
      661 tgccggggcc acgggcgtcg ttcaggcgcc cgggagcctg acgacgaccg cggtgccgcc
      721 aagcgacgag cgctcgaact ctatgccgcc gccgtaggcg ctcaccagct cgttgacgac
      781 cgccagcccg ataccctgcc cgggatggct ttcgtcgagg cgtgcgccgc gcgccatcgc
      841 cgccgaggcg cgctctggat cgagccccgc gccgtcgtcc tcgacccgca gctcgatggc
      901 gcccggcgag atgacggcag cgctgcagcg aatccgtcgg cgtccgtatt tccaggcgtt
      961 gtccagcagg ttgcccagga tttcctgcag gtcgccctcg tcgccgcgga accgcaccgt
     1021 ggcggggatg tcgagctcgc agctgctgcc gcgcccggcg tagaccttgt cgagcgtcgc
     1081 ggcaagatcg cgcgccaccg gggccaccgc cacgggcacg agcccgacgc cgcccgccgc
     1141 gcccgcggcc ccggcgcgac gcagctgcca gccgatgatc tggtccatgc gggagagttc
     1201 cgtgcggatc acccggacgc ggtccgaccg gccatcgtcg acctcgggca acagggtgcg
     1261 caccgccgcc agcggggtct tcaggctgtg ggcaagattg gcgaggctct cctggtagcg
     1321 ctgctggcgc gcccgctcgc ggcgcaacag cgcgttgagg ttggccgcga cgccgcgcaa
     1381 ctcctccggc caggcggccg agagctggtc acgctgcgcg gtctccattt cgtggatctc
     1441 ccgctcgatc cggccgagcg gctgcagcac gcggcgcaac accaccgcca gcacgaacag
     1501 catggccgcg gccagcgcgc cgaaccagcc gaccagaccc agccggaagg cgcggcgctg
     1561 ggcgcgccag ggttccaggc tctggccgac gatgaagcga aacgacgcga cgctttcgtc
     1621 ctcgaactcc cagtcgatgg tgataccggc cagcgccagg ccggggccgt cggactccgc
     1681 ctggacccag cgggcatagg cgccgggtgc gcccgccggg ggcacctcga gttccacccc
     1741 cagtgccgac ggcgagcgcc agacggctgc gccgcgcgca tcgcgaattt cggcgagcag
     1801 gccggagccg gggctcagca tccggccgtc cggcggttcg ggcagcgtca ggccgggcgg
     1861 tgcgtccgcc ggttcggtcg gctcggccat ggcgatcagc gccatcaact gggcctggag
     1921 cacttcgtcg cgtgagcgct cgaggctggt cgcaaggctc cactccagca ctaccaccgt
     1981 ggcgccgaaa aacaccgcca gcgcgatccc gaggctcagc aggacgcgcc ggcggaggga
     2041 gtccttcgcc accggcaatt cagtccttgc ccgccggcgt cagcgcgaaa cgatagccgc
     2101 ggccgcgcag cgtctcgatg ggcttgaggc cgccgtccgg atccagcttg cgacgcaggc
     2161 ggccgacgaa gacctcgatg gtgttgctgt cgcggtcgta gtcctggtcg tagaggcggt
     2221 cgatcagctc gctgcgggaa acgaccttgc cggcattgag caccaggtac tccagcagcc
     2281 ggtactcaaa gccggtgagc tcgaccggct cgccgtgcag ttccacacgt tgcgcggcgg
     2341 gttcgaaccg aagcacgccg aagtcgagca ccggctgcgc ccagcccgcg gcgcggcgca
     2401 ccagcgcctc gaggcgcgcc agcagctcct cggtgtggaa cggtttcacc atgtagtcgt
     2461 cggcccccgc cttgaggccc gccaccttgt cctgccagcg gtcgcgcgcg gtcaggatca
     2521 gcaccggcag ctcgcgccgg ttcttgcgca gggcggagat caggtccatg cccgacatgc
     2581 ccggcaggcc caggtcgacc accgcgacgt ccggggtcga ctcctcagcc aggtacaacc
     2641 cctcgcggcc gtcgccggcc tcgtccacct gccagcctgc ggcgcgcatc gccgccgcca
     2701 ggccgtgccg cagggcttcg tcgtcttcga ccagcaatgc gcgcatttcg ctcgtccccc
     2761 ggcgtgcccc gcgccacgcg ggcgcagggt cgcttcaata gattcgcccg gtctcggcgt
     2821 ccacgtgcca ggtgcgaacg cgcccgtcct cagtgagcac ccggatccgg tgcaccgcgc
     2881 gccccttgtc gcgcttggtc tcggcgcgca gcacctgccc gccggtgcgc tcgctgacca
     2941 tccgggtggc ctccgccagg ctgatgccgt tgttcgcctg ggcgacctgc agcgcctggg
     3001 ccagtgcggg ggtggtcggc gccgtcgccc cggcgaacag cagggccgca caaagcagcg
     3061 ccgccgacgc gccacggcgc aggtgacgac gacgctggag gggaggattg ggcatgtgag
     3121 atccgttccg tgtcatgcaa tttcacaagg gtggaatccc tgccccgcga ttgcaagggg
     3181 gcgggatcca gcccagggcg ggctgcgtcc cgccggacta gaaccgcccc gacggcacga
     3241 ccgtcactcg caccgggatg gtgcggcccg ggcgcgaggc catggtcgtc tggtactcgc
     3301 gaccgccgta cacgtaggtc acccggtagc cctcgacgtg ctcctgcagc tggtagctgg
     3361 tggtcgtctg gcaatgctgc acagtgaccg gccgctgttc gtaggccacg cgacccgggc
     3421 cgccggcgta cacgcgtcgc tcttccgcct gcgcgcccat cgaggcgccg accagggcgc
     3481 ccacggccgt catcgcggcc ttgccgctgc cgccgccgaa ctggctgccg acgacgccgc
     3541 cgatgatgcc gccggcgatg gtcggcaacg gcgaggcgtg gccgctccgt cccgggccgt
     3601 agctgcgggt ctcgatggtc tggtactcgg tttcctgcca gcactcgcgc accggcgttt
     3661 cgacacgcac ctgccggacg atcggcgtga cgtcgacgac gcgcgcgtgg tcgacgcccg
     3721 atttgccgcg gggatcggcc gcggccgtgc ccgcaacgga caccagcagg ccggcgatcg
     3781 ccagtttgag catcagcttg ttgttcatga ccgttctcct cgttctccct gccgccatcc
     3841 ccgcgggatg gtccggcacg ctcgagcaca ggctaggggg ggatcgctga acccagcctg
     3901 aacgctgtgc cccggggtgg caccaggccg gccacctgtc ggccggaacc aggcggattg
     3961 accggcagcc gtccgctgga tagtttttgc cagttcagtt gatgctaaat tgcgcacggc
     4021 gcccggcccc ggcaaacagg cgcgacggcc cttcaagtct tgccggctgc tgccgaaaag
     4081 gaaaatatga aaatgcgctg tttcaggcct gtcccgtcat gacccccgag attcgcgagg
     4141 cgctgcagta ctgcccgaac ctccccagcc cgcccggcat cgccatccgg atcgtcgagc
     4201 tgggccggga cccgaacgtg gacctgaccg ccctgtcccg gctgctagcg cgcgatccgg
     4261 cgctggccag ccgggtgctg cgcgccagca actcggccct ctacgcgcag cgccggcgca
     4321 gcgacaatct ccgccaggcg ctggtggtca tcgggctgaa cgccaccatg accctcgcgc
     4381 tcagcttctc gctggccgaa acgatcaccg accagcccgc gacacgcaac gtgaacaggc
     4441 tcgtctggcg ccgggccctg attgccgcca ccgcggcgcg gctgctcggc acgcgcctga
     4501 agctgcagga actcgaggag ctgtttctcg ccgggctgct gcaggacatc ggcatcctgg
     4561 cgctggacgc cgccattccg gagtcgtacc acccgctggt cgcggaggcc cgcgaccacg
     4621 acgacctgct ggccagggag cgcgcggccc tcgaggcgga tcacggcgac gccggcgaat
     4681 ggctgatgac caactggggg ctgccgaagc gcctggcgcg ggtggcttcg gcggtgcatg
     4741 cgcctgagca ggcaaggctt tcgggcagcg aggcgctgtt cctgaactgc gtcgcggtcg
     4801 ccggcaagat cgccgacctg ttcctgggcg aggatcgcaa ggccggcacg gaccgcgtcg
     4861 cggacgacgc ccggcacctg ctcggcattc gccaggaaga catcgagccg ctgctcaacg
     4921 aggtggcgga agtgctgccg gaagtcgcgg cgctgtacga cacggagatc atctcggccc
     4981 gcaaggcggc cggcgtcatc gaccaggcac gcgagattct cgccgcgcgc aacctgcatc
     5041 tgatccagga agtggcggag cagacccgca aggtgcagga gatggagcgg accacgcagc
     5101 acctgcgcga ctcggcccat cgcgacgcgc tcaccggcct gctcaaccgc ggccgcttcg
     5161 acgaggtgct ggagtccgag ttcatgatgg cgaccgaaag cggctggccg ctgaccatcg
     5221 gcttcatcga tctcgaccat ttcaagtcgg tcaacgacac ccacggccac caggtcggtg
     5281 acgccgtgct gtcgcgggtg gccagcatcc tggcgagcca cctgcgcgag cgtgacttca
     5341 tcatgcgcta cggcggcgag gaattcgtcg cactactgcc cggcacgggc atcgagcagg
     5401 cccagggcgt gttcgagcgg ctgcgcaagg cggtggccga cgagattcac aaaggcgagg
     5461 cgggcgcacg cttccagacc acggtctcga tcggcctggc gacgcatatg gacgggtcgc
     5521 ggcgtgcagc cagcgcggtc gacctggtgc gctcggccga tcgcgcgctg tacgaggcga
     5581 agcgcaccgg ccgcaaccgc atcctggtcg acaagacgat cg
//
LOCUS       DAOMPG010000153         5618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6083_length_5618_cov_3.848104, whole genome shotgun sequence.
ACCESSION   DAOMPG010000153 DAOMPG010000000
VERSION     DAOMPG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_5618_cov_3.848104"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLS34_08795"
     CDS             <1..256
                     /locus_tag="PLS34_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRP87604.1"
                     /translation="VAAFLLLTLAAGLWRVMRGPTAADRMLAAQLFGTTAVAVLLLLA
                     EAMATPSFRDIALVFALLAAVAAVAFVQRVWSRGGGDVGD"
     gene            243..545
                     /gene="mnhG"
                     /locus_tag="PLS34_08800"
     CDS             243..545
                     /gene="mnhG"
                     /locus_tag="PLS34_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRP87605.1"
                     /translation="MSAIDLLSAVLVLVGAGYFFAGTVGMLRFPDVYSRLHALTKGDN
                     VGLGLTIVGLALQAPSLAVAGKLVLIWMLVLLGGATASHLIARASLRRGIKPWTAP"
     gene            542..844
                     /locus_tag="PLS34_08805"
     CDS             542..844
                     /locus_tag="PLS34_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRP87606.1"
                     /translation="MNPGLFLDALLAAILLWLAYTSLSSRDLFRGIVLFIAFGLVLSM
                     TWARLGAPDVALAEAAIGAGLTGALLLATWGRLPGKDGKSKDRPQDPPDKGDEGGG"
     gene            834..1517
                     /locus_tag="PLS34_08810"
     CDS             834..1517
                     /locus_tag="PLS34_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRP87607.1"
                     /translation="MAARRSNGEPGLWLRAGLGLLLLGAAAGLVLVLLTEPLQPGLSD
                     AVAGAMAASGTDNPVTAVLLNFRGYDTLLEIAVLTTTLVGVWALGTAPRITETTPSPI
                     LLGLNSVLLPLAVLIGAYLLWVGALRPGGAFQAGAVLAAAGVLFILAGRRPVTRIPDV
                     PPRWSLVVGLATFLAVAFGVMAAGHRLLEYPGGLAGMLMLVIEAAATLSIAAILIGLF
                     IGGRPEGEP"
     gene            1514..1843
                     /locus_tag="PLS34_08815"
     CDS             1514..1843
                     /locus_tag="PLS34_08815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HRP87608.1"
                     /translation="MTSGLMFAFVGVGLLLIGAWGLALRAHLIRKILAINVMGSGAFL
                     MLVGSGTPAAGTPDPVPQAMVITGIVVAVSATALALALVLRIANATGKPALPEDAERD
                     LMDEEGQ"
     gene            1850..3289
                     /locus_tag="PLS34_08820"
     CDS             1850..3289
                     /locus_tag="PLS34_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP87609.1"
                     /translation="MSGWLAEAPWMLWTIALPLIGAMLSFLFPRRAASLGLAAALATV
                     AAAVVVLLQVVAFGPRLYAVGGWGAPLGIDLLADGLSALMLAMTAVVGAAVSIYARVY
                     LLSSPEKVRRFWPLWMFVWAALNALFQSGDAFNLYVTLELLGLSAVALVALTGSGPAL
                     WGAMRYLLVSLLGSLAYLVGVALLYHAYGTVDLALLGERADGGLALAVPLGAMIAGLL
                     LKTGLFPFHVWLPPAHGSARAPVSALLSALVVKASFYLLLRLWFTFTPEGEGLPLLLG
                     SLGVAAVFWGSVQALRQKRLKLLVAYSTVAQLGYLFIAFPLAGLDPKLAFGGAIYLAL
                     SHALAKGAMFLAAGSIMTQGGHDEIDELDRVVDRMPLTLAGFALAGISLAGLPPSGGF
                     VGKWMLLQAAMAQGQWFWAVAIMIGGVLTATYVFRVLGFAFTKSPHEHERHEVPPTME
                     WTALLLALGAVLLGFVAPWALPLLDGVLP"
     gene            3286..4779
                     /locus_tag="PLS34_08825"
     CDS             3286..4779
                     /locus_tag="PLS34_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP87610.1"
                     /translation="MSTNAWLPLLVLASSLVPGLIIFMLPERAVGWRITLNLGGAVIK
                     IVFLVLMMIGVFHGVDYQFRVPLVPGLDFVLHADALSLMFVNLSAVLWLATTVYAIGY
                     LEDSPNRSRFFGYFSLCVTATVGLAMSGNLLTFLVFYEALTLTTYPLVVHRGTEVARK
                     AGRSYLAHTLFGGVLVLAGTIWLYSLTGTLEFVDRGFIDGRGIDPAVLRIIFVLLILG
                     VGVKAALVPLHSWLPQAMVAPAPVSALLHAVAVVKAGAFGVVRIVYDVFGIEFAASLG
                     VTLPLAVLAAVTIVYGSLRALFQDDLKKRLAFSTVSQVSYITLGIAVAGPIATIGGLV
                     HLVHQGFMKITLFFCAGNYAETLGVHKVSEMRGIGRRMPWTMGAFTLAALGMIGVPPT
                     VGFISKWYLGLGALEVGQDWVVLVLAGSSLLNACYFLPILRTAWFDEPPVEWPHERSF
                     GRRETAWMLLLPPLFTASMVLAWGLFASMPGTPLDWVQFIAARKYAP"
     gene            4776..>5618
                     /locus_tag="PLS34_08830"
     CDS             4776..>5618
                     /locus_tag="PLS34_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP87611.1"
                     /translation="MSLLLALPLLPLAAAAWVALSSRSEQLATGWMVALVAAFPVAIA
                     ALYPPDRLEFPELLVRGDAALVLDPTARAALLLFGGLWLSTGLLLTRTREQGPTTTAL
                     LVGLSGALTLAVAEGGPLVYAGMLATGYGLYAAMASEPGDDWRRSARAFIVLLVASDL
                     LVFELLLSGTAEPGIGTSRKLLLLGLVALALRGGIPPAHAWLPPAMAVVGTPVALLLV
                     AVPPGMALFGALKLLPAGSGEIAVVCALLGLAGAAWVTVAGLAQRQARPTLANALAAS
                     AALLL"
BASE COUNT          625 a         1867 c         2066 g         1060 t
ORIGIN      
        1 cgtcgccgcg ttcctgctgt tgaccctggc tgccggactg tggcgcgtca tgcgcgggcc
       61 gacggcggcc gaccgcatgc tggcggcgca actgttcggc accactgcag tggcggtgct
      121 gctgctgctc gccgaggcca tggccacacc gtcgttccgg gacatcgccc tggtgttcgc
      181 gctgctggcc gcggtcgcgg cggtggcctt cgtacagcgg gtctggagca gggggggcgg
      241 cgatgtcggc gattgacctg ctcagtgccg tgctggtgct ggtcggggcc ggatactttt
      301 tcgccggcac ggtcggcatg ctgcgtttcc cggacgtgta cagccgcctg cacgcgctga
      361 ccaagggcga caacgtcggc ctgggcctca ccatcgtcgg cctcgcactg caggcgcctt
      421 cgctggccgt ggcgggcaag ctggtgctca tctggatgct ggtgctgctg ggcggtgcga
      481 cggcctcgca cctgattgcg cgtgccagcc tgcgacgcgg catcaagccg tggacggcgc
      541 catgaatccc gggctgttcc tcgatgcgct gctggcggcg atcctgctgt ggctcgccta
      601 tacgtcgctc tccagcaggg acctgttccg cggcatcgtg ctgttcatcg ccttcgggct
      661 ggtgttgtcc atgacctggg cccggctggg ggcgcccgac gtcgccctgg cggaggccgc
      721 catcggcgcc gggttgaccg gcgcgttgct gctcgcgacc tggggccggt tgccgggcaa
      781 ggacggcaag tccaaggacc ggccgcagga cccgccggac aagggagacg agggtggcgg
      841 ctaggcggag caatggggag cccgggctgt ggctgcgggc gggcctcggc ttgctgctcc
      901 tcggggcggc tgccggcctg gtgctcgtcc tgctgacaga gccgctgcaa ccggggttga
      961 gtgacgcggt ggcgggcgcc atggccgcca gcggcaccga caacccggtt acggcggtgc
     1021 tgctgaattt ccgcggctac gacaccttgc tggagatcgc ggtgctgacc acgacgctgg
     1081 tgggcgtctg ggcactcggc accgcgccgc gcatcacgga gacgacgccg agcccgatcc
     1141 tgctggggct caacagcgtg ctgctcccgc ttgcggtgct gatcggcgcc tacctcttgt
     1201 gggtgggtgc gttacggccc ggcggcgcct tccaggccgg cgccgtgctg gcggcggcgg
     1261 gggtactgtt cattctcgcg ggccgccggc cggtgacccg gatccccgat gtgccgccgc
     1321 gatggagcct ggtggtcggc ctggcgactt tcctggctgt agcgttcggg gtgatggccg
     1381 ccggccatcg cctgctcgag tacccgggcg gcctcgccgg catgctgatg ctggtgatcg
     1441 aggcggccgc gacgctctcc attgcggcga tcctcatcgg gctgttcatc ggcggaaggc
     1501 cggaggggga gccatgacct ccggcctgat gttcgcgttc gtcggggtgg gactgttgtt
     1561 gatcggcgcc tgggggctgg cgctgcgtgc gcatctcatc cgcaagatcc tggccatcaa
     1621 cgtcatgggc agcggtgcgt tcctcatgct ggtggggtca gggacgccgg ccgccggcac
     1681 ccccgatccg gtgccgcagg cgatggtgat cacgggcatc gtcgtggccg tgtccgcgac
     1741 cgcgctggcg ctggcgttgg tgttgcgcat cgccaatgcg accggcaagc cggcgctgcc
     1801 ggaggatgcg gagcgcgacc tgatggatga ggaggggcag taggcgacta tgagcggatg
     1861 gttggccgag gcaccctgga tgctgtggac gatcgcgctg cccttgatcg gcgccatgct
     1921 cagcttcctg ttcccgcgac gcgccgcctc gctcggcctg gccgcggcgc tggcgaccgt
     1981 cgccgccgcc gtcgtggtgc tgctgcaggt cgtggccttc ggtccgcggc tctacgccgt
     2041 cggcggatgg ggcgcgccgc tcggcatcga cctgctggcg gacggactca gtgcgctgat
     2101 gctggcgatg accgcagtcg tcggggcggc cgtcagcatt tatgcccgcg tgtacctgct
     2161 gagttcgccg gagaaggtac gtcgcttctg gccgctgtgg atgttcgtgt gggcggcgct
     2221 gaacgcactg ttccagtccg gcgacgcgtt caatctctac gtcacactgg agttgctcgg
     2281 cctgtcggcg gtcgcgctgg tggcgctcac cggtagcggg ccggcacttt ggggcgcaat
     2341 gcgttacctg ctggtcagcc tgctcggttc gctcgcctac ctggtggggg tggcgctgct
     2401 ctaccatgct tacggtaccg tcgacctggc gctgcttggc gagcgcgcgg acggcgggct
     2461 ggccctcgcg gtaccgctgg gcgccatgat cgccggcctg ctgctcaaga ccggcctgtt
     2521 tccgttccac gtgtggctgc cgccggcgca cggcagcgcg cgcgcgccgg tcagcgcgct
     2581 gctgtccgca ctggtcgtca aggcctcgtt ctacctcttg ctgcgactct ggttcacctt
     2641 cacgccggag ggcgagggcc tgccgctgtt gctgggttca ctgggtgtgg cggccgtgtt
     2701 ctggggctcg gtgcaggcgc tgcgccagaa gcgactcaag ctgctggtgg cctattcgac
     2761 ggtggcgcag ctcggctacc tgttcatcgc cttcccgctg gcgggcctcg acccgaagct
     2821 cgccttcggc ggcgcgatct acctcgccct gtcgcatgcg ctggccaagg gcgccatgtt
     2881 cctcgctgcc ggcagcatca tgacccaggg cgggcacgac gaaatcgacg aactggaccg
     2941 cgtcgtcgat cgcatgcccc tgacgctggc cggtttcgcg ctcgcgggga tcagcctggc
     3001 ggggctgccg cccagtggcg gcttcgtcgg caagtggatg ctgctgcagg ccgccatggc
     3061 gcagggccag tggttctggg cggtggccat catgatcggt ggcgtgctca ccgccaccta
     3121 cgtgttccgc gtgctcggtt tcgccttcac caagtcgccg catgagcacg agcggcacga
     3181 ggtgccgccc accatggaat ggactgcgct gctgctggcg ctcggtgccg tgctgctggg
     3241 cttcgtcgca ccctgggcgc tgccgctgct cgacggagtg ctgccgtgag caccaatgcg
     3301 tggctgccgc tgctggtgct ggccagctcg ctggttcccg ggctgatcat cttcatgctg
     3361 ccggaaaggg cagtgggctg gcgcatcacg ctgaacctcg gcggggcggt catcaagatc
     3421 gtcttcctgg tgctgatgat gatcggcgta ttccacggtg tggactacca gttccgtgtg
     3481 ccgttggtac cggggctcga cttcgtgctg cacgccgacg cgctgtcgct gatgttcgtc
     3541 aacctgtccg cggtgctgtg gctggccacc accgtgtacg ccatcggcta tctcgaggac
     3601 tcgccgaacc gcagccgctt cttcggctac ttcagcctgt gcgtgaccgc caccgtcggc
     3661 ctggcgatgt cgggcaacct gctcaccttc ctggtgttct acgaggcgct gacgctcacc
     3721 acctatccgc tggtcgtgca tcgcggtacc gaggtggcca ggaaggccgg ccgcagctat
     3781 ctggcgcata cgctgttcgg cggcgtgctg gtgctggccg gcaccatctg gctgtactcc
     3841 ctgaccggca ccctcgaatt cgtcgaccgg ggcttcatcg acgggcgcgg tatcgacccg
     3901 gcggtgctgc gcatcatctt cgtgctgctg atcctcggcg tcggtgtgaa ggcggcgctg
     3961 gtaccgctgc acagctggct gccgcaggcg atggtcgcac cggcgccggt cagcgccctg
     4021 ctgcacgccg tggcggtggt caaggcgggg gccttcggcg tggtgcgcat cgtctacgac
     4081 gtgttcggca tcgagttcgc ggcaagtctc ggcgtgaccc tgccgctggc ggtgctggcc
     4141 gccgttacga tcgtgtacgg atcgctgcgt gcgctgttcc aggacgacct gaagaaacgg
     4201 ctggcgtttt ccaccgtcag ccaggtgtcc tacatcacgc tcggcatcgc ggtggcgggg
     4261 ccgatcgcca ccatcggcgg gctggtgcac ctggtgcacc agggcttcat gaagatcacc
     4321 ctgttcttct gcgccggcaa ctacgcggag acgctgggag tccacaaggt cagcgagatg
     4381 cgcggcatcg gccggcgcat gccctggacc atgggcgcat tcacgctggc ggccctcggc
     4441 atgatcggtg tgccgcccac ggtgggcttc atcagcaagt ggtacctggg cctcggcgcg
     4501 ctcgaggtcg ggcaggactg ggtggtgctg gtgctggccg gcagcagcct gctcaatgcg
     4561 tgttatttcc tgccgatcct gcgcactgcc tggttcgacg agccgccggt ggaatggccg
     4621 cacgagcgca gcttcgggcg ccgggagacg gcgtggatgc tgctgctgcc gccgctgttc
     4681 acggcgtcga tggtgctggc gtgggggctg tttgcctcca tgccgggcac gccgctcgac
     4741 tgggtgcagt tcatcgccgc caggaagtac gcgccgtgag cctgctgctc gccctgccgc
     4801 tgctgccgct ggcggccgcc gcctgggtgg cgctgtcgtc gcgctccgag cagctcgcca
     4861 ccggctggat ggtggcgctg gtggccgcgt tcccggtggc gatcgccgcc ctgtatccgc
     4921 ccgaccggct ggagttcccg gagttgctgg tgcgcggcga tgcggcgctc gtgctggacc
     4981 caacggcgcg cgccgcgctg ctgctgttcg gtggcctgtg gctgagcacc ggcctgctgc
     5041 tgacgcgtac gcgcgagcag ggccccacca cgaccgcatt gctcgtcgga ctgtcgggcg
     5101 cgctgacgct ggcagtcgct gaaggcgggc cgttggtgta cgcgggtatg ctcgccaccg
     5161 gctacggcct ctacgcggcg atggccagcg aacccggcga cgactggcgg cgctccgccc
     5221 gcgcattcat cgtgctgctg gtggccagcg acctgctggt tttcgagctg ctgctgagcg
     5281 gcaccgcaga gccgggcatc gggacgtcgc ggaagctgct gctgctgggg ctggtcgccc
     5341 tggccctgcg cggcggcatc ccgccggcgc acgcctggct gccgccggca atggcggtgg
     5401 tcggcacgcc ggtggcgttg ctgctggtgg cggtgccgcc gggtatggcg ctgttcggcg
     5461 cgctcaagct cctgccggca ggaagcggcg agatcgccgt cgtctgcgca ttgctcggcc
     5521 tcgccggcgc tgcctgggtg acggtcgcag gactggccca gcggcaggcg cggccgacgc
     5581 tggccaacgc acttgccgcc agcgccgcgc tcctgttg
//
LOCUS       DAOMPG010000154         5606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6103_length_5606_cov_4.480094, whole genome shotgun sequence.
ACCESSION   DAOMPG010000154 DAOMPG010000000
VERSION     DAOMPG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6103_length_5606_cov_4.480094"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLS34_08835"
     CDS             complement(<1..811)
                     /locus_tag="PLS34_08835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRP87612.1"
                     /translation="MSDVAEIRNFTMNFGPQHPAAHGVLRLVLEMDGETIQKADPHIG
                     LLHRATEKLAESKPYNQSIGYMDRLDYVSMMCNEHAYVLAIEKLLGVTPPLRAQYNRV
                     MFDEITRILNHLMWLGAHGLDIGAMTMFLYCFREREDLMDCYEAVSGTRMHATYYRPG
                     GVHRDLPLEMPRYEHNRFRSKKVTDKLNEARSGSLLDFIEDFTDRFPACVDDYETLLT
                     DNRIWKQRTVNIGVVTPERAVALGFTGPMLRGSGIAWDLRKKQPYEVYAEMD"
     gene            complement(808..1488)
                     /locus_tag="PLS34_08840"
     CDS             complement(808..1488)
                     /locus_tag="PLS34_08840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRP87613.1"
                     /translation="MSKRSERLAARIRERFGASLELLTGVADELTVVVPPADLLATAL
                     ALRDEEGFEFAMLVELAGVDYLTYGMDEWRTRQATATGFSRGVRRGSPPVSPDTPQRF
                     AVAYHLLSLSLNHRLRVRCHPAPGDPPRLPTVTGIWPAANWFEREAFDLFGIMFEGHP
                     DLRRILTDYGFIGHPFRKDFPLSGQVEVRYDAEQGRVVYEPVDIEPRTLVPRVIRDDH
                     RYDPAIKQ"
     gene            complement(1485..1979)
                     /locus_tag="PLS34_08845"
     CDS             complement(1485..1979)
                     /locus_tag="PLS34_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRP87614.1"
                     /translation="MNAPVPASLGKSQRPGIVTTSVDAVVNWARTGSMWPMTFGLACC
                     AVEMMHAGAARYDLDRFGVIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPRWV
                     ISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALIFGILQLQKKIRRTN
                     TIAR"
     gene            complement(1976..2332)
                     /locus_tag="PLS34_08850"
     CDS             complement(1976..2332)
                     /locus_tag="PLS34_08850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRP87615.1"
                     /translation="MLENYLPILVFLVIASVLGAALLSLGFILGPRRPDDEKTSPYEC
                     GFEAFEDSRMKFDVRYYLVAILFIIFDLEIAFLFPWAVSLDAIGGFGLLAMAVFLLIL
                     VVGFIYEWKKGALEWD"
     gene            complement(2498..2582)
                     /locus_tag="PLS34_08855"
     tRNA            complement(2498..2582)
                     /locus_tag="PLS34_08855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2546..2548),aa:Leu,seq:gag)
     gene            complement(2615..2998)
                     /gene="secG"
                     /locus_tag="PLS34_08860"
     CDS             complement(2615..2998)
                     /gene="secG"
                     /locus_tag="PLS34_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRP87616.1"
                     /translation="MLQTIATVSHVFLAVAIIALVLLQRGKGADAGAAFGAGASGTVF
                     GSRGTTSFLSRTTAILATLFFLTSLGLAYFAGQRTEPGSILDRAPVTAPAEPAPLAVP
                     TPQDADDLPALPATQEPAEQRDPPN"
     gene            complement(3019..3771)
                     /gene="tpiA"
                     /locus_tag="PLS34_08865"
     CDS             complement(3019..3771)
                     /gene="tpiA"
                     /locus_tag="PLS34_08865"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRP87617.1"
                     /translation="MRQKLVAGNWKMYGSRAFTRSLVAGLAAEFANGAPCEIAVCPPF
                     AYLADAVAAAASGPILVGGQDVSAQDEGAFTGEVSGSMLADLGCRFAIVGHSERRSLH
                     GESDELVAGKFLAAQRHGLVPILCVGESLEEREAGQTEAVVGRQLGAVVGAAGIEAFS
                     GAVIAYEPIWAIGTGRTASPEQAQAVHTFIRGLLRAENGTIADFVKVLYGGSVKAANA
                     AELFAMPDVDGGLVGGASLEVAGFASICRSAG"
     gene            complement(3827..5164)
                     /gene="glmM"
                     /locus_tag="PLS34_08870"
     CDS             complement(3827..5164)
                     /gene="glmM"
                     /locus_tag="PLS34_08870"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRP87618.1"
                     /translation="MKRRYFGTDGVRGRVGEPPMTVDFALRLASAAARVLAPGGGTVL
                     IGKDTRLSGYMFEAALEAGLTAAGANVLLAGPLPTPAVAYLCRELGAEFGIVISASHN
                     LYDDNGIKIFAAGGEKLTDEQENAIEALLDEPPLTLESAALGRARRVDHARVDYQEFC
                     KALMPADTNLAGFKLVVDCAHGAAYKVAPRVLAELGAEIIPIGCSPNGRNINEGCGST
                     EPELLATTVKGVGARMGVALDGDGDRCVLVDEHGRLADSDQLLFVLARDAMTRGELRG
                     PVVGTVMSNLGLEQALAELDIPFLRAAVGDRYVMAMLKEHGGQLGGETSGHIINLAKT
                     STGDGLATALQVLAVMQHTGESLASLVAGMRQLPQVMINVKTAQRIDVGGSTSIQAAV
                     RAAEGELGERGRVVLRASGTEPVIRVMVEAVDADEAQRQAGRLAEVVRGAAAH"
     gene            complement(5161..>5606)
                     /gene="folP"
                     /locus_tag="PLS34_08875"
     CDS             complement(5161..>5606)
                     /gene="folP"
                     /locus_tag="PLS34_08875"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRP87619.1"
                     /translation="VCLMHMQGEPRTMQAAPAYGDVVEEVYAFLAARIEACVRAGISL
                     DRIVADPGFGFGKTLEHNLSLMKHLERFGGLGVPLLVGVSRKSMIGAVTGRPVGERLA
                     GGLALAALAVAAGARIIRSHDVAATRDAVTMAAAVSAAGKRGFQG"
BASE COUNT          916 a         1988 c         1839 g          863 t
ORIGIN      
        1 agtccatctc cgcgtagacc tcgtagggct gcttcttgcg caggtcccag gcgatccccg
       61 agccgcgcag catcggcccg gtgaagccta gcgccacggc acgctccggc gtgaccacgc
      121 cgatgttgac ggtgcgttgc ttccagatgc ggttatcagt cagcagcgtc tcgtagtcgt
      181 cgacgcaggc gggaaagcgg tcggtgaaat cttcgatgaa atcgagcagc gagccggagc
      241 gcgcctcgtt cagcttgtcg gtgaccttct tgctgcggaa gcgattgtgc tcgtagcggg
      301 gcatttccag cggcagatcc cggtgcacgc cccccgggcg gtagtacgtg gcgtgcatgc
      361 gcgtgccgga gaccgcctcg tagcagtcca tcaggtcctc gcgctcgcga aagcagtaca
      421 ggaacatcgt catcgcgccg atgtccaggc cgtgcgcccc gagccacatc aggtggttca
      481 ggatgcgcgt gatctcgtcg aacatgacgc ggttgtactg cgcgcgcagc ggcggcgtca
      541 cgccgagcag cttctcgatc gccagcacgt aggcgtgctc gttgcacatc atcgagacgt
      601 agtcgagccg gtccatgtag ccgatcgact ggttgtaggg cttgctctcg gcgagcttct
      661 cggtggcccg gtgcagcagc ccgatgtgcg ggtcggcctt ctggatggtc tcgccgtcca
      721 tctccagcac cagccgcagc acgccgtggg ccgccgggtg ctggggcccg aaattcatgg
      781 tgaagttgcg gatctccgcc acgtcgctca ttgcttgatc gccgggtcgt agcgatggtc
      841 gtcgcgaatc acccgcggca ccagggtgcg cggctcgatg tccaccggtt cgtacaccac
      901 gcgcccctgc tcggcgtcat agcgcacctc gacctgcccg ctgagcggga agtccttgcg
      961 gaacggatgg ccgatgaaac cgtagtcggt caggatgcgg cgcaggtccg ggtgcccctc
     1021 gaacatgatg ccgaacaggt cgaaggcctc gcgctcgaac cagttggcgg cgggccagat
     1081 gccggtgacg gtcggcagcc gcggcggatc gccgggcgcc gggtggcagc gcacccgcag
     1141 ccgatggttg aggctcagcg acagcaggtg ataggcgact gcgaagcgct gcggcgtgtc
     1201 cggcgagacc ggcggcgagc cgcgtcgcac gccgcggctg aaaccggtag cggtggcctg
     1261 ccgcgtccgc cactcgtcca tgccgtaggt caggtagtcc acgcccgcca gttccaccag
     1321 catggcgaac tcgaagcctt cctcgtcccg cagcgccagc gccgtcgcca gcaggtcggc
     1381 cggcggcacc accacggtaa gctcgtccgc caccccggtc agcagttcca gcgatgcgcc
     1441 gaaccgctcc ctgatgcgcg ccgccagccg ttcgcttcgc ttgctcatcg cgcgatggta
     1501 ttggtgcgcc ggattttttt ctgcagctgc agaatgccga aaatcagcgc ctccgcagtg
     1561 gggggacagc cgggcacgta gacgtccacc ggcacgatcc ggtcgcagcc gcgcaccacg
     1621 gcataggaat aatggtagta gccgccgccg ttggcgcagc tgcccatgga gatgacccag
     1681 cgcggctcgg ccatctggtc gtagaccttg cgcagcgccg gcgccatctt gttgaccagc
     1741 gtgccggcga cgatcatcac atccgactgg cgggggctgg ggcgaaagat cacgccgaag
     1801 cggtcgagat cgtagcgcgc cgcgccggcg tgcatcatct cgaccgcgca gcaggcgagc
     1861 ccgaaagtca tcggccacat ggagccggtg cgcgcccagt tgaccacggc gtccaccgaa
     1921 gtggtgacga tgccggggcg ctggctcttg cccagggagg ccggcacggg agcgttcaat
     1981 cccactccag cgcccccttc ttccactcgt agatgaaacc gaccaccagg atcagcagga
     2041 agaccgccat ggcgagcagc ccgaagccgc cgatcgcgtc cagcgacacc gcccaaggaa
     2101 acaggaaggc gatctcgagg tcgaagatga tgaacaggat tgcgaccagg taatagcgca
     2161 cgtcgaactt catgcgcgaa tcctcgaagg cctcgaagcc gcactcgtaa ggcgaggtct
     2221 tctcgtcatc tggtcgacgc ggaccgagaa tgaagcccag ggaaagcagg gcggcgccca
     2281 gcacggaggc gatgacgagg aaaacgagga ttggcagata attctcgagc attcctcttc
     2341 ctgttgagcc cacgcccggc cgagagtgcg gcgaggcggg acactcggcc tattctaaca
     2401 gcgatgcgct gcgttgcagc gccgaagagt cgtgccggaa tacgacccct cgcggaccgc
     2461 gcgggcccgg gctccctcgt ggcgagggtc gagaagatgg tgcggatggt cggactcgaa
     2521 ccgacacggc ttgcgccact gccccctcaa gacagcgtgt ctaccagttc caccacatcc
     2581 gcatgaaaac catacgctcc gcaggcccgg ccgatcagtt cggcggatcc cgctgctctg
     2641 cgggttcctg cgtcgccggc agtgccggca ggtcgtccgc atcctgcggc gtgggcacgg
     2701 cgaggggcgc gggctccgcc ggcgccgtca ccggcgcgcg atccaggatg ctgccaggct
     2761 cggtacgctg cccggcgaag taggcgagcc cgaggctcgt caggaaaaac aacgtcgcga
     2821 ggatggccgt cgtcctgctg aggaacgagg tcgtgccgcg cgatccaaac acggtcccgg
     2881 aggcgccggc accgaaggcc gcacccgcat cggcgccctt gccgcgctgg agtagcacca
     2941 gggcgatgat cgccacggcg agaaagacgt ggcttactgt ggcaatggtc tgcaacattt
     3001 gattccggtt tccgcagctc agccggccga acggcagatg ctcgcgaagc ccgcgacctc
     3061 gagagaggcg ccgcccacga gcccgccgtc aacgtccggc atggcaaata gttcggctgc
     3121 attggcggct tttacgctgc cgccgtacag caccttgacg aagtcggcaa tcgtaccatt
     3181 ttccgcgcgc aacaggccac ggataaatgt gtgcaccgcc tgggcctgct ccggcgaggc
     3241 ggtgcggccg gtgccgatgg cccagatggg ctcgtaggcg atcaccgcgc ccgagaaggc
     3301 ctcgatgccg gccgcgccga ccacggcgcc aagctgccgg ccgacgaccg cctcggtctg
     3361 cccggcttcc cgttcctcca ggctctcgcc cacgcacagg atcggcacca gcccgtggcg
     3421 ctgcgcggcc aggaacttgc ccgccaccag ttcatcgctc tcgccatgca ggctgcgccg
     3481 ctcggaatgg cccacgatcg cgaatcgaca gccgaggtcc gccagcatgc tgcccgacac
     3541 ctcgccggtg aacgcgccct cgtcctgcgc cgagacgtcc tggccaccga ccaggatcgg
     3601 cccgctcgcg gccgccgcaa cggcgtcggc caggtaggcg aacggcgggc acacggcgat
     3661 ttcgcagggt gcgccgttgg cgaattccgc cgccaggccg gccaccagcg agcgcgtgaa
     3721 cgcccgcgag ccatacatct tccagttgcc tgcgaccagc ttctgtctca tcccggtctc
     3781 cggaccgcgt aaaggcgcgg cactttagcc gcaggcccgt ggaaaatcaa tgtgctgcgg
     3841 cgccccggac cacctcagcc aggcgcccgg cctgccgttg cgcctcgtcc gcgtcgaccg
     3901 cctccaccat gacgcggatc accggctcgg tgccggaggc acgcaacacc acgcggccgc
     3961 gctcgcccag ttcgccctcg gccgcgcgca ccgcggcctg gatcgacgtc gaaccgccca
     4021 cgtcgatgcg ctgcgcggtc ttcacgttga tcatcacctg gggcagctgg cgcatgcccg
     4081 cgaccagcga ggccagtgac tcgccggtat gctgcatcac cgcgagcacc tgcagcgcgg
     4141 tggcgaggcc gtcgccggtg gaggtcttcg ccaggttgat gatgtggccg gaagtctcgc
     4201 cgccgagctg cccgccgtgt tccttgagca tggccatcac gtagcgatcg ccgaccgctg
     4261 cgcgcaggaa cggaatgtcc agctccgcca gcgcctgctc gagcccgagg ttgctcatca
     4321 cggtgcccac caccgggccg cgcaattcgc cgcgcgtcat ggcatcgcgt gcgagcacga
     4381 acagcagctg gtcgctatcc gccagccggc cgtgctcgtc caccagcacg cagcggtcgc
     4441 cgtcgccgtc cagcgccacg cccatccggg cgccgacgcc cttgacggtg gtcgccagca
     4501 gctcgggctc ggtggagccg cagccctcgt tgatattgcg gccgttgggt gaacagccga
     4561 tggggatgat ttccgccccc agttcggcca gcacgcgtgg cgcgaccttg taggcggcgc
     4621 cgtgggcgca gtccaccacc agcttgaagc cggcgagatt cgtatccgcc ggcatcagcg
     4681 ccttgcagaa ctcctggtag tccaccctgg cgtggtccac ccggcgcgcg cgccccagcg
     4741 ccgccgactc gagcgtcagc ggcggctcgt cgagcagggc ctcgatggcg ttctcctgct
     4801 catccgtgag cttttcgccg cccgcggcga aaatcttgat gccgttgtcg tcgtacaggt
     4861 tatgggaggc gctgatcacg atgccgaact cggcgccgag ctcgcggcac aaataagcca
     4921 ccgccggcgt cggcagcggc ccggccagca gcacgttggc gcccgccgcc gtcaggccgg
     4981 cctccagtgc cgcctcgaac atgtagccgg acagccgcgt gtccttgccg atcagcaccg
     5041 tgccgccgcc gggggccagc acgcgtgccg ccgcgctcgc cagccgcagc gcgaaatcca
     5101 cggtcatcgg cggctcgccg accctgccgc gcacgccgtc ggtgccgaaa tatcgcctct
     5161 tcatccctgg aatccccttt tgcctgccgc gctcaccgcg gctgccatgg tcaccgcatc
     5221 acgggtcgcg gcgacgtcat ggctgcggat tatccgcgcc ccggcggcca ccgccaatgc
     5281 cgccagcgcc agccccccgg ccagtcgctc gccgaccggg cggcccgtga cggctccgat
     5341 catggacttt ctcgatacgc cgaccagcag cggcacaccg agcccgccaa aacgctcgag
     5401 gtgcttcatc aggctgaggt tgtgctccag cgtcttgccg aaaccgaaac ccgggtcggc
     5461 gacgatgcga tccagggaaa tcccggcgcg cacgcaggcc tcgatgcgcg cggcgaggaa
     5521 agcgtatacc tcctccacca cgtccccgta ggccggcgcc gcctgcatgg tgcggggctc
     5581 gccctgcata tgcatcaggc agaccg
//
LOCUS       DAOMPG010000155         5573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6148_length_5573_cov_3.552918, whole genome shotgun sequence.
ACCESSION   DAOMPG010000155 DAOMPG010000000
VERSION     DAOMPG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6148_length_5573_cov_3.552918"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1099)
                     /locus_tag="PLS34_08880"
     CDS             complement(155..1099)
                     /locus_tag="PLS34_08880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP87620.1"
                     /translation="MAAKRDDQKQAGKGGKGGKGGKGGKGAAGKGSKGAAGKGKPKVR
                     MADLADRHELYEASVQSVSHEVEFLATAFEELVGRKPVSLREDFCGTAAAACEWVRSD
                     RRRTAVAVDFDPEVLAWGRAHHVAALKPKQRERVRLIEGDVRSAETGPADVTVAFNFS
                     WWTFKTREALLGYFRRVHAALADDGLFVLDIYGGSDAYAEQEEETDYGLFTYVWDQHR
                     FDPVSARYICYIHFRFPDGSELPRAFRYDWRLWTLPELRELLAEAGFARSVVYWQGED
                     EDGEPSGEFSIVERGDADPAWIAYVAAVKGSGRRGGKG"
     gene            1207..>2417
                     /locus_tag="PLS34_08885"
     CDS             1207..>2417
                     /locus_tag="PLS34_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRP87621.1"
                     /translation="MSRCLPAALALSLLSAASMPAFAAEPSPALLKEAATLRDAARAE
                     SDAWRHAASLTTEVGPRFAGSVGDRAAVAWALANLADMGFDEVRPQTVIVPGWTRGEA
                     EVRITQPFPQPLVAVALGSTPGTPEEGIEAPVIRFESLAALEAARPSAVRGHIVFIDE
                     RMERTRDGSGYGPAVQKRSRGPSVASERGAVAMVIRSVGTSNNRLAHTGNTRFAEGVR
                     PIPAFALSAPDADMLTRQIETGRDVLLKLRSSARLLPPERSANVIADIRGAEKPDEIV
                     ILAAHLDCWDVGTGAHDNATGVAILMETARYLLAQSRRPARTVRLVLYANEEAGLDGS
                     KTYTAAALEAGLRHVVGIEADAGGGVIYRFNSGVGEAALPVVEAMRDVLAPLDIKGGD
                     KVVKALDEQFK"
     assembly_gap    2418..2427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2428..2743
                     /locus_tag="PLS34_08890"
     CDS             <2428..2743
                     /locus_tag="PLS34_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87622.1"
                     /translation="EAMRELLAPLGIEGGDNSARGGADLSTLRAEGMPVLALQHDMSE
                     YFDLHHTANDTLDKVDPDALKQAVAAYASVVWIAANVEGDFGSFPEPAVPPADPGETT
                     TR"
     gene            complement(2810..3784)
                     /gene="dusA"
                     /locus_tag="PLS34_08895"
     CDS             complement(2810..3784)
                     /gene="dusA"
                     /locus_tag="PLS34_08895"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRP87623.1"
                     /translation="MDQKLPRVRPAVAVAPMMEWTDRHCRFFLRLLSRHALLYTEMVT
                     AQAVLHGDRERLLGFDPAEAPLALQLGGSEPATLAQAALAGAHYGYDEINLNVGCPSD
                     RVQSGRFGACLMAEPALVRDCVAAMQAAVDIPVTVKSRIGIDDRDDYGFLRDFVGTVA
                     ESGCDTFIVHARKAILAGLSPKENREVPPLRYEFVYRLKQEIPQLNIMLNGGVRDLDA
                     VRAHLAKVDGVMIGREAYHNPYFLAELDRELHGVEPPTREAVMTAFMDYVEARLAEGL
                     RLTAMTRHVLGLYLGRPGARRWRRQLSEGACARGAGLEVIRAAMRLVA"
     gene            3918..4214
                     /locus_tag="PLS34_08900"
     CDS             3918..4214
                     /locus_tag="PLS34_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP87624.1"
                     /translation="MQHERRHSARSEVATDVYLDVDGIRLRGRALNLSPRGVFITGVQ
                     PRLEAGRVVDLVFPVPVQGMIRLHRKRAVVAHASPRGIGMRMEPAPRPLHVAGR"
     gene            complement(4320..5465)
                     /locus_tag="PLS34_08905"
     CDS             complement(4320..5465)
                     /locus_tag="PLS34_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRP87625.1"
                     /translation="MSHKEPSFTVGVEEEYLLVDKESRDLVTDMPESMIEECESAMEA
                     RGQVSPEFLRAQIEVGTKVCESIAEVRQELSRMRECVGKVADKHGCAPIAASTHPFAR
                     WLDQRHTDKERYDSLGRELQATARRLLICGMHVHVGIEDEETRIDLMSQFAYFLPHLL
                     ALSCSSPFWEGENTGLKSFRLTVFDALPRTGMPENFASWAEYSRHIEVMVEAGLIEDG
                     TKIWWDVRPSARYPTLETRVMDVCTRIEDAICIAAINVSLLRMLFRLRRSNQRWRTYA
                     RMLLMENRWRAMRYSFDEGLVDLGRGAIVPHAENLGELIELIREDSDALGCTAEVEHA
                     WKILERGTSAHRQVATYERALAAGAAPREALNAVVDMLIEESRVGPA"
BASE COUNT          807 a         1984 c         1887 g          885 t
ORIGIN      
        1 cgtcggcaaa caggacggcg actgaaaatt cactggacat cgtgttccgg ggcctgttcc
       61 ctgcggcctg cctggaggga ggcaaatccg tgctgagcat actcgctgga ccgctggcca
      121 atcaactcct tacgcgcgct agctgaggtt ttcatcagcc tttgccgccg cgcctgccgg
      181 agcccttcac ggccgcgaca taggcgatcc acgccgggtc ggcgtcgccg cgctcgacga
      241 tggagaactc gccgctcggc tcgccgtcct cgtcctcgcc ctgccagtac accaccgagc
      301 gggcgaaacc ggcctcggcc agcagttcgc gcagctccgg cagcgtccac agccgccagt
      361 cgtagcggaa ggcgcgcggc agctcggagc cgtccgggaa gcggaagtgg atgtagcaga
      421 tgtagcgcgc cgacaccggg tcgaagcggt gctggtccca gacgtaggtg aacaggccgt
      481 agtcggtctc ctcttcctgc tcggcatagg cgtccgagcc gccgtagatg tcgagcacga
      541 acaggccgtc gtcggccagc gcggcatgca cgcggcggaa gtagccgagc agcgcctcgc
      601 gcgtcttgaa ggtccaccag ctgaagttga aggccacggt gacgtcggcc ggcccggtct
      661 cggcgctgcg cacgtcgccc tcgatcagcc gcacgcgctc gcgctgcttc ggcttcagcg
      721 ccgccacgtg gtgcgcgcgt ccccaggcca gcacctccgg gtcgaagtcc accgccaccg
      781 ccgtgcggcg ccggtcgctg cgcacccatt cgcaggccgc cgccgccgtg ccgcagaagt
      841 cttcgcgcag gctcaccggc ttgcggccga ccagctcctc gaacgccgtg gcgaggaact
      901 cgacctcgtg gctgacgctc tgcaccgagg cctcgtagag ctcatgacgg tcggcgagat
      961 ccgccatgcg gaccttgggc ttgcccttgc cggcggcgcc cttgctgccc ttgccggcgg
     1021 cgcccttgcc gcccttgccg cccttgccgc ccttgccgcc cttgcctgcc tgcttctggt
     1081 cgtcgcgctt cgccgccatg ccgtgtcacc gccccgctcc gtggaaggcg ggcattctag
     1141 ccgaatgcgg cggcgttgat tggtgtacat taagggcccg cattccccga cctcggagtc
     1201 cgctcgatgt cacgctgcct gcccgcggcg ctcgccctgt cattgctgag tgccgcctcc
     1261 atgcccgctt tcgccgccga gccctcgccc gcactgctca aggaggccgc cacgctgcgt
     1321 gacgccgcgc gcgccgagag cgacgcctgg cgccacgccg cgtcgctgac cactgaggtc
     1381 gggccgcgtt tcgcggggtc ggtgggcgat cgcgccgccg tggcctgggc gctggccaac
     1441 ctcgccgaca tgggcttcga cgaggtccgc ccgcagacgg tgatcgtgcc gggctggacg
     1501 cgcggcgagg ccgaggtgcg catcacccag cccttcccgc agccgctggt ggcggtggcg
     1561 ctgggcagca cgcccggcac gccggaggaa ggcatcgagg cgccggtgat ccgcttcgag
     1621 agcctcgccg cgctggaggc cgcccggccc tcggcggtgc gcggccacat cgtgttcatc
     1681 gacgagcgca tggagcgcac gcgcgacggc agcggctacg gcccggccgt gcagaaacgc
     1741 agccgcgggc cgtcggtggc gtcagagcgc ggcgcggtgg cgatggtgat ccgctcggtc
     1801 ggcacctcga acaatcgtct cgcgcatacc ggcaacacgc gttttgccga gggcgtgcgg
     1861 ccgatcccag ccttcgcgct gtccgcgccg gatgcggaca tgctgacgcg ccagatcgag
     1921 acgggccgcg acgtgctgct gaagctgcgc tcgagcgcgc ggctgctgcc gccggagcgt
     1981 tcggcgaacg tgatcgccga catccgcggt gccgagaagc cggatgagat cgtcattctc
     2041 gccgcgcacc tcgactgctg ggacgtcggc accggcgcgc acgacaacgc caccggcgtg
     2101 gccatcctga tggagaccgc gcgctacctc ctcgcgcagt cgcggcggcc ggcgcgcacc
     2161 gtgcggctgg tgctgtacgc caacgaggaa gcggggctgg acggctccaa gacctacacg
     2221 gctgcggcgc tggaggccgg cttgcggcac gtggtgggga tcgaggcgga tgccggcggc
     2281 ggcgtgatct accgcttcaa cagcggtgtc ggcgaggcgg cgctgccggt ggtcgaggcg
     2341 atgcgtgacg tgctggcgcc actcgacatc aagggcggcg acaaggtcgt caaagccctc
     2401 gacgagcagt tcaaggannn nnnnnnncga agccatgcgt gaactgcttg cgccgctcgg
     2461 catcgagggc ggcgacaaca gcgcgcgcgg cggcgccgac ctgtcgacgc tgcgtgccga
     2521 gggcatgccg gtgctggcgc tgcaacacga catgagcgag tacttcgacc tgcaccacac
     2581 cgccaacgac acgctcgaca aggtcgaccc cgatgcgctg aagcaggccg tcgcggccta
     2641 cgcctcggtg gtgtggattg cggccaacgt cgagggtgat ttcggttcct tcccggaacc
     2701 ggcggtgccg ccggccgatc cgggcgagac cacgacgcgc tgaagccggg cgccgacgcg
     2761 ggtacttctg ccagggccgc agcggatcgc cgccgaaccg gcgtggccgt caggcgacca
     2821 gccgcatggc cgcgcggatg acctcgaggc cggcgcccct ggcacaggcg ccttccgaga
     2881 gctggcggcg ccagcggcgc gcacccggcc ggccgagata caggccgagc acgtgccgcg
     2941 tcatggcggt cagccgcaag ccctcggcga gccgcgcctc gacgtagtcc atgaacgccg
     3001 tcatgaccgc ctcgcgcgtc ggcggctcga cgccatgcag ctcgcgatcc agctcggcca
     3061 ggaagtacgg gttgtggtac gcctcgcggc cgatcatcac gccgtcgacc ttggccaggt
     3121 gcgcgcgcac cgcgtcgaga tcgcgcacgc cgccgttgag catgatgttc agctgcggga
     3181 tttcctgctt gaggcggtag acgaactcgt agcgcaatgg cggcacctcg cggttttcct
     3241 tcggcgacag gccggcgagg atcgccttgc gcgcgtgcac gatgaaggta tcacagccgg
     3301 actcggccac cgtgccgacg aagtcgcgca ggaagccgta gtcgtcgcga tcgtcgatgc
     3361 cgatgcggct ttttaccgtg acggggatgt ccacggcggc ctgcatggcg gcgacgcagt
     3421 cgcgtaccag cgcaggctcg gccatcaggc aggcgccgaa gcggcctgac tgcacgcggt
     3481 cgctcgggca gccgacgttg aggttgatct cgtcgtagcc gtaatgcgcg ccggccagtg
     3541 cggcctgggc gagcgtcgcg ggttcggacc cgccgagctg cagcgccagc ggcgcctccg
     3601 ccggatcgaa gccgagcagc cgctcgcggt cgccgtgcag caccgcctgc gccgtcacca
     3661 tctccgtgta cagcagcgcg tggcggctca gcagccgcag gaaaaagcgg cagtggcggt
     3721 ccgtccactc catcatcggc gccaccgcca ccgcgggtcg gaccctcgga agtttctgat
     3781 ccatttgcat ttgccgcgga ttttgcgcga ttccgagggc ttagaacaat gatttcgggc
     3841 ccgaatcggg catctaaggc acggccggcg ccccaaggga attcagattc ctacgccgtt
     3901 tctgtattct cggcgacatg cagcacgagc ggcgtcactc agcacgaagc gaagtcgcaa
     3961 ccgatgtgta cctcgacgtc gacggaattc gcctgcgtgg ccgtgcgctc aacctgtcgc
     4021 cgcgcggcgt ctttatcacc ggcgtgcagc cgcggctcga ggccggccgc gtggtcgacc
     4081 tggtctttcc ggtgcctgtg cagggcatga tccggctgca tcgcaagcgc gcggtcgtcg
     4141 cgcacgcctc gccgcgcggc atcgggatgc gcatggagcc cgcgccgcga ccgctgcacg
     4201 tcgccggccg gtgaagtcct tccgccccgt ctcttagacg caccgttttc gcccgaaaac
     4261 cgtgctctaa ggccctgttt tgcccctgtt ttcgacctaa ctgattgatt cgaatggagc
     4321 tacgcgggtc cgacccgcga ctcttcaata agcatgtcga ccacggcgtt caaggcctcg
     4381 cgcggcgcgg cgccggcggc cagcgcgcgc tcgtaggtcg ccacctggcg gtgggcgctg
     4441 gtgccgcgct cgaggatctt ccacgcgtgc tcgacctcgg cggtgcagcc gagcgcgtcc
     4501 gaatcttcgc ggatcagctc gatcagttcg ccgaggttct cggcatgcgg cacgatcgcg
     4561 ccgcgcccga ggtccaccag cccctcgtcg aagctgtagc gcatcgcccg ccagcggttc
     4621 tccatcagca gcatgcgcgc gtaggtccgc cagcgctggt tcgagcgccg cagccggaac
     4681 agcatgcgca gcagcgacac gttgatcgcc gcgatgcaga tggcgtcctc gatgcgcgtg
     4741 cacacgtcca tcacgcgcgt ctccagcgtc gggtagcgcg cgctcggccg cacgtcccac
     4801 cagatcttgg tgccgtcctc gatcagcccg gcctccacca tcacctcgat gtgccgcgag
     4861 tactccgccc agctggcgaa attctccggc atgccggtgc gcggcagcgc gtcgaacacc
     4921 gtgaggcgga acgacttcag cccggtgttc tccccttccc agaacggcga cgagcacgac
     4981 agcgccagca ggtgcggcag gaaatacgcg aactggctca tcaaatcgat gcgcgtctct
     5041 tcgtcctcga tgccgacgtg cacatgcatg ccgcagatca gcagccgccg cgcggtggcc
     5101 tgcagctcgc ggccgaggct gtcgtagcgc tccttgtcgg tgtggcgctg gtcgagccag
     5161 cgggcgaagg gatgcgtgga ggccgcgatc ggcgcgcagc cgtgcttgtc cgccaccttg
     5221 ccgacgcatt cgcgcatgcg cgacagctcc tggcgcacct cggcgatgct ctcgcacacc
     5281 ttggtgccca cctcgatctg cgcgcgcagg aactcggggc tgacctggcc gcgcgcctcc
     5341 atggcgctct cgcattcctc gatcatcgac tcgggcatgt ccgtgacgag gtcgcggctc
     5401 tccttgtcga cgagcaggta ttcctcctct acgccgacgg tgaagctcgg ttccttgtgg
     5461 ctcatgtcag ccctccccgt atttcctgta cagcccctcg tcggcgagga tccgctcgag
     5521 cacgtcgccg aggatgtccg cccagcgcga gacgccctcg gggtgatcga tca
//
LOCUS       DAOMPG010000156         5520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6224_length_5520_cov_3.829277, whole genome shotgun sequence.
ACCESSION   DAOMPG010000156 DAOMPG010000000
VERSION     DAOMPG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_5520_cov_3.829277"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..1143)
                     /gene="pgeF"
                     /locus_tag="PLS34_08910"
     CDS             complement(394..1143)
                     /gene="pgeF"
                     /locus_tag="PLS34_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRP87626.1"
                     /translation="MIPAWMVPDWPAPPAVRAVSTLRPGGASPPPWSSLNLAEHVGDD
                     PARVARNRDLLARALGLPAAPAWLEQVHGCDVLRLDQAELPAARVADGAVTARTGRVL
                     AVLTADCLPVLFCRADGKAVGIAHAGWRGLAAGVLEATVAAMDAPPERLLAWIGPGIG
                     AAAYEVGDEVRAACLAADDGADEHFAPGRPGHWQFDLAGLARNRLAALGLAGVHGGEW
                     CTHSDPARFFSHRRDGAERPTGRMATLIWLS"
     gene            complement(1140..2090)
                     /gene="rluD"
                     /locus_tag="PLS34_08915"
     CDS             complement(1140..2090)
                     /gene="rluD"
                     /locus_tag="PLS34_08915"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HRP87627.1"
                     /translation="MQTVEHRVVVPAELAGQRLDQALAALFPDYSRSRLQGWIKAGRV
                     RVDGRELRPRDKVEGGEAIVLEAALEDETRAEAQAIRLAILHEDEQILVIDKPAGLVV
                     HPGAGNPAGTLVNALLHHRPDLRALPRAGIVHRLDKDTTGVMVVACTPGAHAALVRQL
                     EARSVKREYQAICVGVMTGGGTVDAPIGRHPVDRVRMAIREGGREAVTHYRVIERFRG
                     HTHVLCRLETGRTHQIRVHLASIRNPIVGDPVYGGRLALPKGASPALIEALRGFRRQA
                     LHAARLELVHPGSGEQVHFEAPLPADFEGLLAALRADLAA"
     gene            2222..2977
                     /locus_tag="PLS34_08920"
     CDS             2222..2977
                     /locus_tag="PLS34_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRP87628.1"
                     /translation="MTQRLASLLLAVALATTAGCASWRNKDVTADPTSNAQRIYEQSV
                     GAMKAGNYGAAILGFENLTAIYPFSEEARQALLNLIYVYWRNDEPDNAVSAADRFILE
                     NPTHPRVDYALYMKGLARFPRDAGPLERLFRTDLTERPTGQMLSAYNTFAQLLQQHPE
                     SAYEEDARQRMIYLRNRLAAHEVNVARFYVERHAHVAAINRARHVLESYQETPSAIEA
                     LRIIATAYDNLGLEDLAADTRRILAANEAARRR"
     gene            complement(2990..4597)
                     /locus_tag="PLS34_08925"
     CDS             complement(2990..4597)
                     /locus_tag="PLS34_08925"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRP87629.1"
                     /translation="MRIAVAQVNPVVGDVETNVTRMLEVCRRAHDALRADLVIFPELA
                     LCGYPPEDLLFHRGFRRDVAEALARLGDSLDGMSVLVGYPEYAGEEIFNSAILLRPDG
                     PPVVYRKQTLPNYGVFDEKRYFSPGTQPCVVEMKGVRLGVTICEDLWGEDVARRTVAA
                     GAEVLLSINGSPYDTGKLARREAVLRARSTETGLPIIYVNIAGGQDELIFDGGSMAVS
                     AAGEIVYRAPFFTEALEIIEVERGGRIGPARVQPAPEFVEGVYRALELGVADYVNKHN
                     FPGVVLGLSGGIDSALTLAVAVDALGPERVRAVMMPSRYTSQMSREDAQRQAELLGVR
                     YDVVSIEPIFEGARQALAEVFRGMPEDVTEENIQARCRGMILMAMSNKFGCMLLTTGN
                     KSEMAVGYATLYGDMAGGFAPLKDCSKTLVYRLAEYRNRNVEVIPRRVIERPPSAELR
                     PGQRDTDSLPPYEVLDPILEAFIEEDCSVDQIVARGYERAVVGRILGMVKRNEYKRRQ
                     APPGIRISRRAFGRDWRYPITSGFAAR"
     gene            complement(4660..>5520)
                     /gene="sucD"
                     /locus_tag="PLS34_08930"
     CDS             complement(4660..>5520)
                     /gene="sucD"
                     /locus_tag="PLS34_08930"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRP87630.1"
                     /translation="LVDRNSKVICQGFTGRQGTFHSEQCIEYGTQMVGGVTPGRGGEK
                     HLDLPVFDTVHDAVAETGADVSMIYVPAPFAADAILEAADAGIGLVVCITEGIPVLDM
                     VKVKAALKDYDCRLIGPNCPGIITPGQAKIGIMPGFIHKPGKVGIVSRSGTLTYEAVY
                     QTTRNGLGQSTCVGIGGDPVRGMNFIDCLALFEQDEQTEGVLMVGEIGGSDEEAAAEF
                     ISQHMTKPVVAYIAGVTAPPGKRMGHAGAIVSGGKGTADEKFRALEAAGVTTVRSPAE
                     LGSAILERLG"
BASE COUNT          812 a         1995 c         1904 g          809 t
ORIGIN      
        1 gtcagtttgt ccatgcgcat gacgggggtc ccggtagtcc ttggctctcc ctgatattgg
       61 ggtcagaccc cggttttcaa ccagctggcg cgtcctttcg ggtcggccgc gggggcggac
      121 catcatgggc tcccctcgct ccgaactctt atgtcgctgc ggggagccca tgatgtccca
      181 cccccgctac cgtgccgaac ggggtggcgc cgtctggcgc aaccgcctgg tgacacgccg
      241 ctaccatcgc ggcagcgcca aggcctacct gtcggcgggg tggcagcggc ggggggatgg
      301 cacaggtccg cggagcgcat aaagatttct aagcgaggcg gacctgtgcc gtctccccgc
      361 cgcggtcgac ccgaggcacc ggaacgccgc gaatcagctg agccagatca gcgtcgccat
      421 ccgcccggtc ggccgctccg cgccgtcgcg gcggtgcgag aagaagcgcg ccgggtccga
      481 gtgcgtacac cattcgccgc catgcacgcc ggccagcccc agcgcggcca gccggttgcg
      541 cgccagcccg gccaggtcga actgccagtg gcccgggcgg ccgggcgcga agtgctcgtc
      601 ggcgccgtcg tcggccgcca ggcaggcggc gcggacctca tcgccgactt cgtaggcggc
      661 ggcgccgatg ccgggcccga tccaggcgag cagccgctcg ggcggggcat ccatggccgc
      721 aacagtggcc tcgaggacgc cagcggcaag gccgcgccag ccggcgtggg cgatgccgac
      781 cgccttgccg tcggcgcggc agaacagcac cggcaggcag tctgcggtca gcaccgcgag
      841 gacccgcccg gtgcgcgcgg tgacggcgcc gtcggcaacg cgcgcggccg gcagctcggc
      901 ctggtcgaga cgcagcacgt cgcagccgtg cacctgctcg agccaggcgg gcgcggcagg
      961 caggcccagc gcccgcgcca gcaggtcgcg gttgcgggcc acccgcgcgg ggtcgtcgcc
     1021 gacatgctcg gcgaggttga gtgaactcca gggcggcggg ctggcgccgc cggggcgcag
     1081 tgtgctgacg gcccgcaccg ccggcggcgc cggccagtcg ggcaccatcc aggccgggat
     1141 catgccgcca ggtccgcccg cagcgccgcg agcaggccct cgaagtcggc gggcagcggc
     1201 gcctcgaagt gcacctgctc accgctgccc gggtgcacca gttccagccg tgcggcatgc
     1261 agggcctggc gccggaagcc gcgcagggcc tcgatcaacg ccgggctggc gcccttcggc
     1321 aaggccaggc gaccgccgta caccgggtcg ccgacgatcg ggttgcggat cgaggcgagg
     1381 tggacgcgaa tctggtgggt gcggcccgtt tccagccggc acagtacgtg ggtgtggccg
     1441 cgaaagcgct cgattacccg gtagtgggtc accgcctcgc ggccgccctc gcggatggcc
     1501 atgcgcaccc ggtcgaccgg gtgccggccg atcggtgcgt cgacggtgcc gccgccggtc
     1561 atcacgccga cgcagatcgc ctggtactcg cgctttacgc tgcgcgcctc gagctggcgt
     1621 accagcgccg catgcgcgcc cggcgtgcag gcgaccacca tgacgccggt ggtgtccttg
     1681 tcgagccggt gcacgatgcc ggcgcgcggc agcgcccgca ggtccggccg gtgatgcagc
     1741 agcgcgttga ccagcgtgcc cgccggattc ccggcgcccg ggtgcaccac cagccccgcg
     1801 ggcttgtcga tcaccaggat ctgttcgtcc tcgtgcagga tcgccagccg gatcgcctgc
     1861 gcctcggccc gggtctcgtc ctccagcgcg gcctccagca cgattgcctc gccgccttcc
     1921 accttgtcgc gcggccgcag ttcacggccg tcgacgcgca cccggccggc cttgatccag
     1981 ccctgcagcc ggctgcggga atagtccggg aacagcgcag ccagcgcctg gtcgaggcgc
     2041 tgcccggcca gttctgcggg cacgacgacg cggtgctcga cggtctgcat cagggaaccc
     2101 cgtggtggcc tgcgggtcgg aaaagccaag tgtacggcct cgccgggcgc cacgtccggc
     2161 gcagcgggta taatcggccg ccgccgctcc cggccgcgac cgcctgatgg aacaacattc
     2221 gatgacccaa cgtctcgcca gcctgttgct cgctgttgcg ctcgccacga ccgccggctg
     2281 cgcatcctgg cgcaacaagg acgtcacggc cgaccccacg agcaacgccc agcgcatcta
     2341 cgagcaatcg gtcggcgcca tgaaggccgg caactacgga gcggcgatcc tcggcttcga
     2401 gaacctcacg gcgatctacc cgttcagcga ggaggctcgc caggcgctgc tgaacctcat
     2461 ctacgtttac tggcgcaacg acgagcccga caatgccgtc tccgcggccg accgcttcat
     2521 cctcgagaac ccgacccacc cgcgggtcga ttatgcgctg tacatgaagg gcctggcgcg
     2581 cttcccgcgc gacgccggcc cgctcgagcg gctgttccgc acggacctga ccgagcggcc
     2641 gaccgggcag atgctgagcg cctacaacac tttcgcgcag ctcctgcaac agcatcccga
     2701 gagcgcctac gaggaagacg cgcgccagcg gatgatctac ctgcgcaacc ggctcgcggc
     2761 ccacgaggtg aacgtggcac ggttctacgt cgagcggcat gcgcacgtcg cggcgatcaa
     2821 ccgcgcgcgg cacgtgctgg aaagctacca ggagacccct tcggccatcg aggcgctgcg
     2881 tatcatcgcg acggcctacg acaatctcgg cctggaggac ctggccgcgg acacccgccg
     2941 gatcctggcc gccaacgagg ccgcccggcg ccgctgagac cggccggtct cagcgagcgg
     3001 caaagcccga ggtgatcggg tagcgccagt cgcgcccgaa ggcgcgccgg ctgatgcgga
     3061 tgccgggcgg cgcctggcgg cgcttgtact cgttgcgctt gaccatgccg agaatgcgcc
     3121 cgaccacggc acgctcgtaa ccgcgcgcga cgatctggtc caccgagcaa tcctcctcga
     3181 tgaaggcctc caggatcggg tcgagcacct cgtagggcgg cagcgagtcg gtgtcgcgct
     3241 ggccgggccg cagctccgcc gacggcggcc gctcgatgac ccgccgcggg atcacctcga
     3301 cgttgcggtt gcgatactcg gccaggcggt agaccagggt cttgctgcaa tccttcagtg
     3361 gcgcgaagcc gccggccatg tcgccgtaca gggtcgcgta accgaccgcc atctcgctct
     3421 tgttgccggt ggtcagcagc atgcagccga acttgttcga catcgccatc aggatcatgc
     3481 cgcggcagcg cgcctggatg ttctcctcgg tgacgtcctc cggcatgccg cggaacactt
     3541 ccgccagggc ctgcctggcg ccctcgaaga tcggctcgat ggacaccacg tcgtaacgca
     3601 cgccgagcag ctcggcctgc ctttgcgcat cctcgcggct catctggctg gtgtaacgcg
     3661 acggcatcat caccgcccgg acccgctcgg ggccgagcgc gtccaccgcc acggccagcg
     3721 tcagcgccga gtcgatgccg ccggacagcc cgagcaccac gccgggaaag ttgtgcttgt
     3781 tgacgtagtc ggcgacgccg agctccagtg cgcggtaaac gccttcgacg aactccgggg
     3841 ccggctggac cctggccggc ccgatgcgcc cgccccgctc cacttcgatg atttccagcg
     3901 cctcggtgaa gaacggcgcg cgatagacga tctcgcccgc ggcgctcacc gccatggaac
     3961 cgccgtcgaa aatcagttcg tcctgcccgc cggcaatatt gacgtagatg atcggcaggc
     4021 cggtctcggt gctgcgggcc cgcagtacgg cctcgcgccg cgccagcttg ccggtgtcgt
     4081 agggcgagcc gttgatggac agcagcacct cggcgccggc cgcgaccgtg cggcgcgcca
     4141 cgtcctcgcc ccagaggtcc tcgcagatcg tgaccccgag gcgcacgccc ttcatctcga
     4201 ccacgcaggg ctgcgtgccg ggcgagaaat agcgcttctc gtcgaatacg ccgtagttcg
     4261 gcagggtctg cttgcggtac accaccggcg ggccgtcggg gcgcagcagg atggcgctgt
     4321 tgaagatctc ttcgccggcg tattcggggt agcccaccag caccgacatg ccgtcgaggc
     4381 tgtcgccgag ccgcgccagc gcttccgcca cgtcgcgccg gaagccgcgg tggaacagca
     4441 ggtcctcggg cgggtagccg cacagcgcca gctcggggaa gatcaccagg tcggcgcgca
     4501 aggcgtcgtg cgcgcggcgg cagacttcca gcatgcgcgt gacgttggtc tcgacgtcgc
     4561 cgacgacggg gttgacctgg gcgacggcaa tgcgcatggc gcggaaactg gggtcagaca
     4621 ccatttttgc acgcaaaaat ggtgtctgac cccagtttgc taccccagcc gctcgaggat
     4681 ggcgctgccc agctcggccg gcgaacgcac cgtggtgacg ccggccgcct cgagggcgcg
     4741 gaacttttcg tccgccgtgc ccttgccgcc cgagacgatc gcgccggcgt ggcccatgcg
     4801 cttgcccggc ggcgccgtga cgccggcgat gtaggcgacc accggcttgg tcatgtgctg
     4861 ggagatgaat tccgccgcgg cttcctcgtc gctgccgccg atctcgccca ccatcagcac
     4921 gccctcggtc tgctcgtcct gctcgaacag cgccaggcag tcgatgaagt tcatgccgcg
     4981 caccgggtcg ccgccgatgc ccacgcaggt cgactggccg aggccgttcc tggtggtctg
     5041 gtaaaccgcc tcgtaggtca gcgtgccgga gcgcgacacg atgccgacct tgcccggctt
     5101 gtggatgaag cccggcatga tgcctatctt cgcctgcccg ggggtgatga tgccggggca
     5161 gttggggccg atcaggcggc agtcgtagtc cttgagcgcc gccttgacct tcaccatgtc
     5221 gagcaccggg ataccctcgg tgatacacac caccagcccg atgccggcgt cggcggcctc
     5281 gaggatggcg tcggcggcga agggcgccgg cacgtagatc atgctgacgt ccgcgccggt
     5341 ctcggcgacg gcgtcatgca cggtgtcgaa caccggcagg tcgagatgct tttcgccgcc
     5401 gcgacccggc gtcacgccgc cgaccatctg cgtgccgtac tcgatgcact gctcggaatg
     5461 gaatgtgccc tgccggccgg tgaagccctg gcagatgacc ttgctgttgc ggtctacgag
//
LOCUS       DAOMPG010000157         5471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6299_length_5471_cov_4.651219, whole genome shotgun sequence.
ACCESSION   DAOMPG010000157 DAOMPG010000000
VERSION     DAOMPG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_5471_cov_4.651219"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLS34_08935"
     CDS             <1..733
                     /locus_tag="PLS34_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRP87631.1"
                     /translation="HLAELPGAGYAYACVLAGVFITALYSFRMFFLVFHGPERFDAHT
                     RSHLHEPPAVVTGPLVLLAIPSVIAGWLGMEALLFGGFFGDAIQVSPARDTLAALAAG
                     GADSPTAMLAHALVTPALWLALAGLGVAWYLYLKRPELPGRIAARLGGLVRVLDQKYY
                     FDAFNERVLAPMARGAGGLFWRMGDGALIDGLLVNGSARAVGWLSGVVRRVQTGYLYH
                     YAFAMFIGLALMIGWLLFVGARGQG"
     gene            738..2252
                     /locus_tag="PLS34_08940"
     CDS             738..2252
                     /locus_tag="PLS34_08940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRP87632.1"
                     /translation="MPTSLPLLSILVWLPILGGLAVLAVGDGRAPLARRLSLLIAALT
                     FVASLPLWIRFDPTTAAMQFTERAPWIPAFDAWYHLGVDGISMPLILLTTFITVLVVI
                     AGWEVIKSRPAQYFASFLVLEGVMIGVFAALDALLFYVFWEAMLIPMFIIIGVWGGAR
                     RVYATLKFFLYTFLGSVFMLVALIYLRVEAGSYELLALHRLPLGMTEQTLIFLAFLAA
                     FAVKVPMWPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPITPDASHALDW
                     LLIGLSLVAVVYVGFVALAQNDMKKLIAYSSIAHMGFVTLGFFIAWRILANTGGADGI
                     VMGLQGGMVQMVSHGLISGALFLCVGVLYDRMHSREISAYGGVANTMPVFAAFLVLFA
                     MANAGLPGTSGFVGEFLVILASFKASFWYAALAATTLVLGAAYTLWMVKRVLYGEVAN
                     EQVAALKDIGRREFLVLGVLALAVLLLGLWPAPLIDAMATTLQGLADHVVQSKL"
     gene            2249..3682
                     /gene="nuoN"
                     /locus_tag="PLS34_08945"
     CDS             2249..3682
                     /gene="nuoN"
                     /locus_tag="PLS34_08945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRP87633.1"
                     /translation="MNGMSTFAPATAEIFLATAACLVLLADLFLPERRRVLSYWLAMA
                     TLLGTALSLLATAPEGRIVALAGAFVADPLSQLLKLFSCGVVAVAFLYARSYLQLRGL
                     FKGEYYVLGLFATLGIFVMISAHSMLSLYLGLELMSLSLYAMVAFNRDSPLAAEAAMK
                     YFVLGAIATGCFLYGTSILYGVTGSLDLGEIARRAGELNGLDIHLLVALSFIIVGVAF
                     KFGAVPFHMWLPDVYQGASTPVTMFIGTAAKIASLAMIIRVLVEGLGAMHASWADMLV
                     VLAVLSMAIGNIVAIAQTNLKRMLAYSTISHVGFILLGILAGTPAGYQAAVYYTLAYV
                     IMAGAAFGMIILLSRQGFEADELSDFSGLNARSPWFAAIMLIVMFSMAGVPPLLGFYA
                     KFAVLAAVVEVGRTWLAVAGVVFSVIGAYYYLRVVKLMYFDAPGDAGPLAAPADLRWV
                     LSLNGLAVLALGIFPQALVAVSIRALG"
     gene            3794..3870
                     /locus_tag="PLS34_08950"
     tRNA            3794..3870
                     /locus_tag="PLS34_08950"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3828..3830,aa:Met,seq:cat)
     gene            3996..4448
                     /gene="rimP"
                     /locus_tag="PLS34_08955"
     CDS             3996..4448
                     /gene="rimP"
                     /locus_tag="PLS34_08955"
                     /inference="COORDINATES: protein motif:HMM:NF000927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRP87634.1"
                     /translation="MTLKDKLLELLAPEVAALGYELVELDAPAAGGSGTLRIYIDREE
                     GIGLDDCERVSHRVSGLLDVEDPIPGHYVLEVSSPGLDRPLRTEEHFRRQAGQLAKIV
                     LVAGRPGRRRYKGSIVGANDGMVEIEVDGERIALSLSDIESARLVPEF"
     gene            4472..>5471
                     /gene="nusA"
                     /locus_tag="PLS34_08960"
     CDS             4472..>5471
                     /gene="nusA"
                     /locus_tag="PLS34_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRP87635.1"
                     /translation="MNKEILQVIEQVSAEKELDKEIIFEALEMALATAARKQFGEDID
                     TRVAMDRKAGTLSTFRRWKVFADDSTELETPSRELRLEDALDIDPNAEVGGFVEEPLD
                     VTPTGRIVAQTAKQVVVQKVREAERAQVVEEFRDRVGTLIGGIVKRVDRNGVFVDLGG
                     NAEGFISREEMIPREAVRTQDRIKGYLREVRSEPRGPQIFLSRTAPEFIIELFKLEVP
                     EVGQGLIKILGAARDPGHRAKIAVKSLDQRIDPIGACVGMRGSRVQAVSNELAGERVD
                     IIPWDDNPAQFVINAMSPAEVVSIVVDEEAHSMDVAVEEDKLSQAIGRGGQNVRLAS"
BASE COUNT          766 a         1766 c         1895 g         1044 t
ORIGIN      
        1 ccaccttgcc gagctgccgg gcgccggtta cgcctatgcc tgcgtgctgg ccggggtgtt
       61 catcaccgcc ctgtacagct tccgcatgtt cttcctggtg ttccacgggc cggagcgctt
      121 cgacgcgcac acccgcagcc acctgcacga gcccccggcg gtggtgaccg gcccgctggt
      181 gctgctggcc atcccgtcgg tgatcgccgg ctggctgggc atggaggcgc tgctgttcgg
      241 cggctttttc ggcgacgcca tccaggtctc gcccgcgcgc gacacgctcg cggcgctggc
      301 cgcgggcggc gccgattcgc ccaccgccat gctggcgcac gccctcgtga ctcccgcgct
      361 ctggctggcg ctggcgggcc tgggtgtggc ctggtacctg tacctcaagc ggcccgagct
      421 gcccggccgg atcgccgccc ggctcggcgg cctcgtgcgc gtgctggacc agaaatacta
      481 tttcgacgct ttcaacgagc gcgtgctggc gccgatggcg cggggcgccg gcggcctgtt
      541 ctggagaatg ggcgacggcg cgctgatcga cggcctgctg gtcaacggct cggcccgggc
      601 ggtgggctgg ctgtccgggg tcgtccgccg ggtgcagacc ggttacctgt accactacgc
      661 cttcgccatg ttcatcggac tggcgttgat gatcggttgg ctgctgttcg tcggcgcgcg
      721 ggggcaggga taacgccatg ccgaccagcc tgccgctgct gagcattctc gtctggctgc
      781 cgattctcgg cggcctcgcc gtgctcgcgg tcggtgacgg gcgcgcgccg ctggcgcgcc
      841 ggttgtcgct gctgatcgcc gcgctgactt tcgtggccag cctgccgctg tggatccgct
      901 tcgacccgac cacggccgcc atgcagttca ctgagcgggc gccgtggatc cccgccttcg
      961 acgcctggta ccacctgggc gtggatggca tttccatgcc gctgatcctg ctgaccacct
     1021 tcatcacggt gctggtggtg atcgccggct gggaggtcat caagagccgg cccgcgcagt
     1081 acttcgcgtc cttcctggtc ctggaggggg tcatgatcgg cgtgttcgcc gcgctcgacg
     1141 cgctgctgtt ctacgtcttc tgggaagcga tgctgatccc gatgttcatc atcatcggcg
     1201 tctggggcgg cgcgcggcgc gtctacgcca cgctcaagtt tttcctgtac accttcctcg
     1261 gctcggtgtt catgctggtc gcgctgatct acctgcgggt cgaggcgggc agctacgagc
     1321 tgctggcgct gcaccggctg ccgctcggga tgaccgagca gactctcatc ttcctggcct
     1381 tcctggcggc gttcgcggtc aaggtgccga tgtggccggt gcacacctgg ctgcccgacg
     1441 cccatgtcga ggcgcccacg ggcggctcgg tgatcctggc ggccatcatg ctgaagatgg
     1501 gcggctacgg cttcctgcgc ttcagcctgc cgatcacccc ggacgccagc cacgcgctgg
     1561 actggctgct gatcggcctg tcgctggtgg cggtggtcta cgtcggcttc gtggcgctgg
     1621 cgcagaacga catgaagaag ctgatcgcgt actcctcgat cgcccacatg ggcttcgtga
     1681 cgctcggctt tttcattgcc tggcggatcc tggccaatac cggtggggcg gacggtatcg
     1741 tcatgggcct gcagggcggc atggtccaga tggtgtccca cgggctgatc tccggcgcgc
     1801 tgttcctgtg cgtcggcgtg ctctatgacc gcatgcacag ccgcgagatc tccgcctacg
     1861 gcggcgtggc caacaccatg ccggtgttcg cggccttcct ggtgctgttc gccatggcca
     1921 acgccggcct gccgggcact tccggcttcg tcggcgagtt cctggtgatc ctggccagct
     1981 tcaaggccag cttctggtac gcggcactgg ccgccaccac gctggttctc ggcgccgcct
     2041 acacgctgtg gatggtcaaa cgcgtgctgt acggcgaggt ggccaacgag caggtggcgg
     2101 cgctcaagga catcgggcgg cgggaatttc tggtgctggg cgtcctcgca ctggcggtgc
     2161 tgttgctggg cctgtggccg gcgccgctca tcgacgccat ggcgaccacc ctgcagggcc
     2221 tcgccgacca cgtggtgcaa tccaagctat gaacggcatg agcacattcg cccctgcgac
     2281 ggcggagatc ttcctggcaa ctgcggcctg cctcgtgttg ctcgcggacc tgttcctgcc
     2341 cgagcgccgc cgggtgctga gctactggct ggccatggcg acgctgctgg ggaccgcgtt
     2401 gagcctgctg gcgaccgcgc ccgagggccg catcgtggcc ctggcgggcg ccttcgtggc
     2461 cgacccgcta tcgcagctgc tgaagctgtt cagctgcggc gtggtagccg tggccttcct
     2521 ctatgcgcgc agctacctgc agctgcgggg gctgttcaag ggcgagtact acgtgctcgg
     2581 gctgttcgcc acgctgggca tcttcgtgat gatctcggcg cacagtatgc tcagcctgta
     2641 cctcggcctg gagctgatgt ccctgtcgct gtacgccatg gtggccttca atcgcgacag
     2701 cccgctggcg gcggaagcgg ccatgaaata cttcgtgctg ggggcgatcg ccacgggctg
     2761 cttcctgtac ggtacttcga ttctttacgg cgtgaccggg agcctggatc tcggcgagat
     2821 cgcgcggcgc gccggcgagc tgaacgggct agatatccac ctgctggtgg ccttgtcctt
     2881 catcatcgtg ggcgtggcct tcaagttcgg cgccgtgccg ttccacatgt ggctgcccga
     2941 cgtgtaccag ggcgccagca cgccggtcac gatgttcatc ggcacggccg caaagatcgc
     3001 ctcgctggcg atgatcatcc gcgtgctggt ggaggggctc ggcgccatgc acgccagctg
     3061 ggcggacatg ctggtggtgc tggcggtgct ctccatggcg atcggcaaca tcgtcgccat
     3121 cgcgcagacc aacctgaagc ggatgctggc ctactcgacg atctcgcacg tcggcttcat
     3181 tttgctgggc atcctggccg gcacgccggc cggctaccag gcggcggtct actacacgct
     3241 ggcctacgtg atcatggccg gcgccgcctt cggcatgatc atcctgctca gccggcaggg
     3301 cttcgaggcc gacgagctga gcgatttcag cggcctgaac gctcgcagcc cctggttcgc
     3361 ggccatcatg ctgatcgtaa tgttcagcat ggcgggcgtg ccgccgctgc tgggtttcta
     3421 tgccaagttc gccgtgctcg cggccgtggt cgaagtgggg cgcacctggc tggccgtggc
     3481 cggcgtggtg ttttcggtca tcggcgccta ttactacctg cgtgtcgtca agctgatgta
     3541 tttcgatgca ccaggcgacg ccggccccct ggcggcgcct gccgacctgc gctgggtgct
     3601 gagcctgaac gggctggccg tgctggccct gggcatcttc ccgcaggcgc tggtcgccgt
     3661 cagtatccgc gccctgggct gagcaattgg ggacattccc caattccatg cggaattggg
     3721 gaatgtcccc aattggtgcc cgcatttagc ttgcactgcg gaaaagcccc ccgtaaacta
     3781 tgcggtctgc ggatgcgggg tggagcagtc tggcagctcg tcgggctcat aacccgaagg
     3841 tcgcaggttc gaatcctgcc cccgctacca agcttttcgc cggaaaaggc ccgctgggcc
     3901 tttttcttta tccggccccc tgcggggggt tgggaatggc ctccaagggg gctgacaggt
     3961 gggccgcagg gcccattttt tgttttggca agacagtgac gctgaaggac aaattgctgg
     4021 agttgctggc gccggaagtg gcggccctgg gctacgagct ggtcgaactc gatgccccgg
     4081 cggccggtgg gtcgggaacg ctgagaattt acatcgaccg ggaagagggt atcgggctgg
     4141 acgattgcga gcgggtcagc caccgggtga gcgggctgct ggacgtcgag gaccccatcc
     4201 cgggccacta cgtcctcgag gtgtcctcgc cgggcctgga ccggccgctg cgcaccgagg
     4261 agcattttcg ccgccaggcg ggacagctgg caaagatcgt gctggttgca gggcgaccgg
     4321 gacggcggcg ctacaaggga tcgatcgtcg gcgcgaacga cggcatggtt gaaattgagg
     4381 tggatggcga gcgcattgcg ctgtcgctgt ctgatatcga atccgcccgg ctggtgccgg
     4441 agttctagtc ctggggaccg ggtcaactga aatgaacaag gaaatcctgc aggttatcga
     4501 acaggtgtcg gccgaaaagg aactcgacaa ggagatcatt ttcgaggccc tggagatggc
     4561 gctggcgacc gccgcacgca agcagttcgg cgaagacatc gatacgcgcg tcgccatgga
     4621 ccgcaaggcc ggcacgctga gcactttccg tcgctggaag gtgtttgccg acgattccac
     4681 ggagctggag acgccgagcc gtgagttgcg actcgaagat gcgctcgaca tcgacccgaa
     4741 cgccgaagtc ggcggcttcg tcgaggaacc gctggacgtc acgccgaccg gtcggatcgt
     4801 cgcccagacc gccaagcagg tggtggtgca gaaggtccgg gaggccgagc gcgcccaggt
     4861 ggtcgaggag ttccgcgatc gcgtcggtac gctcatcggc ggcatcgtca agcgggtgga
     4921 ccgcaacggc gtgttcgtgg acctgggcgg caacgccgag ggcttcatct cccgcgagga
     4981 gatgatcccg cgcgaggccg tgcgtaccca ggaccgcatc aagggctacc tgcgcgaggt
     5041 gcgttccgag ccgcgcggcc cgcagatttt cctgtctcgc accgcaccgg agttcatcat
     5101 tgaactgttc aagctcgagg tgccggaggt cggccagggc ctgatcaaga ttctcggtgc
     5161 cgcgcgcgat ccgggccacc gcgccaagat cgcggtgaag agcctcgacc agcggatcga
     5221 cccgatcggc gcctgcgtcg gcatgcgcgg ctcgcgcgtg caggccgtgt ccaacgagct
     5281 ggccggcgag cgggtcgaca tcatcccgtg ggacgacaac ccggcgcagt tcgtcatcaa
     5341 cgccatgtcg cctgccgaag tggtttccat cgtggtcgac gaagaagcgc acagcatgga
     5401 cgtggcggtc gaggaggaca agctgtcgca ggccatcggc cgcggcggcc agaacgtgcg
     5461 cctggcgagc g
//
LOCUS       DAOMPG010000158         5456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6324_length_5456_cov_3.449732, whole genome shotgun sequence.
ACCESSION   DAOMPG010000158 DAOMPG010000000
VERSION     DAOMPG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_5456_cov_3.449732"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLS34_08965"
     CDS             <1..201
                     /locus_tag="PLS34_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87636.1"
                     /translation="LPGITGSDLGVTDARLLSPGDPGRSLVWLRMGRRDAHAMPPIGS
                     LEVDEDGLALVSAWIVGLAGCD"
     gene            complement(215..949)
                     /gene="dnaQ"
                     /locus_tag="PLS34_08970"
     CDS             complement(215..949)
                     /gene="dnaQ"
                     /locus_tag="PLS34_08970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRP87637.1"
                     /translation="MRQIVLDTETTGLEPAEGHRVIEIGCVELVNRRPTERTFHQYLN
                     PEREIDRAAEDVHGLSNAFLADKPRFHEVAAEFLAFIEGAELVIHNAAFDVGFLDAEL
                     ARLEPRGPAIAEVCGVLDTLMLARQTHPGQRNSLDALCKRYSVDNSGRDLHGALLDAR
                     LLAEVYLAMTGGQAELGLAADTGGGATTGPRRRKKVNRKGLSLVVQRASAEEIAAHEA
                     ALDRLDKALGEPCLWRRAAPGSSAEP"
     gene            complement(1049..1504)
                     /gene="rnhA"
                     /locus_tag="PLS34_08975"
     CDS             complement(1049..1504)
                     /gene="rnhA"
                     /locus_tag="PLS34_08975"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRP87638.1"
                     /translation="MTRVEAWTDGACRGNPGPGGWGALLRSGGHERELNGAEGMTTNN
                     RMELTAAIRALESLKRPCIVDLYTDSQYVRGGITEWLAGWKRNGWRTSGRQPVKNQDL
                     WQALDAAAARHQVRWHWVKGHSGHPENDRADALANAAIDALAGPTRVSN"
     gene            complement(1501..2205)
                     /locus_tag="PLS34_08980"
     CDS             complement(1501..2205)
                     /locus_tag="PLS34_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP87639.1"
                     /translation="MPIYPPELSEWLQSPTGMRLLAAERAVAAEPLRRVFGSQLLQIG
                     AWGGAEEFVGLAGTVRRAVCAAEQAPGIGFVAEDWQLPVASHSVDAVLLPHTHERSSE
                     PHQLLRECERVLAGGGRLIVLGFNPWSVVGLRRLLSRGGWPPQIEQLYSERRLRDWLS
                     LLNFDVTGVQRYFPAFAEAEGRFRHRARGLPGYYGGYLLVAVKRVHAVTPLRRLWRRP
                     ERLPTGLAEPTTRSRL"
     gene            2347..3120
                     /gene="gloB"
                     /locus_tag="PLS34_08985"
     CDS             2347..3120
                     /gene="gloB"
                     /locus_tag="PLS34_08985"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRP87640.1"
                     /translation="MLKVTPVPAFRDNYIWLVHGDDPRRVAVVDPGDARPVIAAVEAQ
                     ALEPVAVLLTHHHPDHSGGIPALRDAFDIEAWGPAGEPVAGLSHTVSGGERVELPALG
                     LAFDVIDIPGHTAGHIAFHGHGALFCGDTLFSAGCGRIFEGTPAQMLASLDALAALPD
                     DTRVYCGHEYTGANLRFAQAVEPANADVAAHVEKVAALRRDDTPSLPSTLAIEKRINP
                     FLRCDQPAVQAAASRRATQPLASRVEVFAAVRRWKDEFA"
     gene            3117..4865
                     /locus_tag="PLS34_08990"
     CDS             3117..4865
                     /locus_tag="PLS34_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRP87641.1"
                     /translation="MRTADEAPTASCLVLRAAGRLACIAALLSLAACAAPRPEAPAES
                     AAPGDELIVETEATLADALDDGRDDHGHDDDDRAEDRSIDSETTSAARPEAHSAAEVV
                     QAFEAVDERIEDLFEQLRGGYALPAVDNDAVRRHADWYGRHPDHLERVLNRARPYLPH
                     VAAELSARGLPAELAFIPVIESAFDPFAYSHGRAAGLWQIVPGTGRRFGLKQNWWYDG
                     RRDVVESTRAALDYFEYLHKFFDGDWLLAVAAYNSGEGTVRRAIRSNQDAGRPTDFWS
                     LRLPAETRSYVPRLLGLAAVLADPEAYSLSLPSVAPTPAFAKVELDGQIDLAVAAELA
                     GIELADLQALNPGFNRWATDPDGPHRLLLPPDAEASFLAALAELPPTERVRWHRHQVA
                     AGDTLGGIARRYRTTVRVLQSVNGLTGTNIRLGSHLMVPTSTRSLDDYPLSAANRLAS
                     TQQRERSGRVRAEHTVRSGESLWTISRKHGVGVRELAAWNGMAPGDTLAAGRKLVVWQ
                     QGDAASAARAPASNATLRRVHYTVRQGDSLYRIANNFRVSVDDIKRWNKLDGKRYLQP
                     GQRLILHVDVTAQSGG"
     gene            4877..>5456
                     /locus_tag="PLS34_08995"
     CDS             4877..>5456
                     /locus_tag="PLS34_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004660733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87642.1"
                     /translation="MAFLQGKRVLIVGLASERSIAWGIAQAMRREGAELAFTYQGEKL
                     KPRVEDMAAQLGGGFTCAMDVAEDEQITGAFEALGRHWDGLDVIVHSVGFAPRDQLSG
                     RFVDAVNREGFRIAHDISAYSFAALAQAGLPLMRGRGGSLLTLSYLGAVRAVPNYNVM
                     GPAKASLEATVRFLAADLGAEGLRVNGISAWPI"
BASE COUNT          799 a         2003 c         1876 g          778 t
ORIGIN      
        1 ctgccgggca tcacgggcag cgatctgggc gtcacggatg cacgcctgct gagcccgggc
       61 gacccgggcc ggtcgctggt ctggctgcgc atggggcggc gcgatgcgca tgccatgccg
      121 ccgatcggca gcctcgaagt ggacgaggac ggcctggcgc tggtcagcgc gtggatcgta
      181 ggcctggccg ggtgcgactg agttccaggc gcagctaggg ctcggcggag gacccaggcg
      241 ccgcccgtcg ccacaggcac ggctcgccca gggccttgtc cagccggtcg agcgcggcct
      301 catgcgcggc gatttcctcg gcgctggcgc gttgcactac caggctgagg cccttgcgat
      361 tgaccttctt gcgccgccgc ggaccggtgg tggcgccacc gccggtgtcg gccgccaggc
      421 cgagttccgc ctggccgccg gtcatcgcga ggtacacctc ggcaagcagg cgtgcgtcga
      481 gcagtgcgcc gtgcaggtcg cgacccgaat tgtcgacgct gtagcgcttg cacagcgcgt
      541 cgaggctgtt gcgctggccg ggatgcgtct ggcgggccag catgagggtg tcgagcacgc
      601 cgcacacctc ggcgatcgcg ggcccgcgcg gctcgaggcg cgccagctcg gcgtcgagaa
      661 agcccacgtc gaaggccgca ttgtggatca ccagctcggc gccctcgatg aaggcgagaa
      721 actccgcggc cacctcgtgg aagcgtggct tgtccgcgag gaaggcgttc gacaggccgt
      781 gcacgtcctc ggcggcacgg tcgatctcgc gctcgggatt gaggtactgg tggaaggtgc
      841 gctcggtcgg acgccggttg accagctcca cgcacccgat ctcgatgact cgatggccct
      901 ccgccggctc gagcccggtg gtctcggtgt ccaggacgat ctgtctcatg ccgtctccgc
      961 ctggccgcgc ttagcgcccc gatttttgcc caaaaactgc attctaagcc ccgaaaacag
     1021 cccgttttca caccaaatct ttttcatttc agttgcttac ccgggtcgga cccgcgagag
     1081 catcgatggc ggcgttggcg agggcgtcgg cgcggtcgtt ttccgggtgg ccgctgtggc
     1141 ctttcaccca gtgccagcga acctggtggc gggccgccgc ggcatcgagc gcctgccaca
     1201 ggtcctggtt cttgaccggc tgccgccccg acgtgcgcca gccgttccgc ttccagcccg
     1261 ccagccactc agtgatgccg ccgcgcacgt actgcgagtc cgtgtagagg tccacgatgc
     1321 agggccgctt gagcgactcc agcgcccgga tggccgcggt cagttccatg cggttgttgg
     1381 tggtcatgcc ctcggcgccg ttgagctcac gctcatggcc gcctgaccgc agcaacgccc
     1441 cccagccgcc cggaccggga ttgccgcgac atgccccatc cgtccaggcc tcgactcgcg
     1501 tcaaagccga ctccgcgtgg tgggctccgc cagcccggtc ggcagccgct cggggcgccg
     1561 ccacagccgg cgcagcggcg tcaccgcgtg cacgcgcttc accgccacca gcaggtagcc
     1621 accgtagtag ccgggcagcc cgcgcgcgcg gtggcggaag cgcccctccg cctctgcgaa
     1681 cgctgggaaa taccgctgca cgccggtcac gtcgaagttc aacagcgaca accagtcgcg
     1741 caggcgccgc tcggaataca actgctcgat ctgtggcggc cagccgccgc gcgacagcag
     1801 ccgtcgcaga ccgaccacgc tccatggatt gaagccgagc acgatcagcc ggccgccccc
     1861 ggccagcacg cgctcgcact cgcgcagcaa ctggtgcggc tcgctggagc gctcgtgcgt
     1921 gtgcggcagc agcacggcgt cgaccgagtg gctcgccacc ggcaactgcc agtcctccgc
     1981 cacgaaaccg atccccggcg cctgctccgc ggcacacacc gcgcgccgca cggtgccggc
     2041 cagcccgacg aattcctccg caccgcccca cgcgccgatc tgcagcaatt gtgagccgaa
     2101 cacccgccgc agcggctcgg cggccaccgc ccgctccgcc gccagcaggc gcatgccggt
     2161 cggcgactgt agccactcgg acaactcggg gggatagatc ggcatgccgg ctaggatagc
     2221 ccaactgcga aacagggaca tgccggaatt ggggacagtc cccaatttcc caattttccg
     2281 aaaattgggg actgtcccca attccgcggg ttggccggga cggtcatccg cccgtaaaat
     2341 cgccgcatgc tcaaggtgac gccggtaccg gccttccgcg acaactacat ctggctcgtg
     2401 cacggcgacg atccgcgccg cgtggccgtc gtcgatcccg gcgacgcgcg cccggtcatc
     2461 gcggcggtcg aggcgcaggc gctggagccg gtcgccgtcc tgctgaccca tcaccatccc
     2521 gaccactccg gcggcattcc cgcgctgcgc gacgccttcg acatcgaggc ctggggaccg
     2581 gccggcgagc cggtggcggg cctcagccac accgtctccg gcggcgagcg cgtcgagttg
     2641 cccgcgctcg ggctggcctt cgacgtcatc gatatcccgg gccacaccgc cggccacatc
     2701 gcttttcacg gtcacggcgc gctgttctgc ggcgacacac tgttcagcgc cggctgcggc
     2761 cgcatcttcg aaggcacgcc ggcgcagatg ctggcctcgc tggatgcgct cgcggcgctg
     2821 cccgacgaca cccgtgtcta ctgcggtcac gagtacaccg gcgccaacct ccgctttgcg
     2881 caggcggtcg agccggccaa cgccgacgtc gccgcgcatg tcgagaaggt cgccgccctg
     2941 cgccgcgatg acacaccgag cctgccgtcg acgctggcta tcgagaagcg catcaatccc
     3001 ttcctgcgct gcgaccagcc ggcggtgcag gcagccgcca gccgccgggc cacgcagccg
     3061 ctggcctcgc gcgttgaagt gttcgcggcg gtgcgccgct ggaaggacga gttcgcatga
     3121 ggacggcgga cgaagcgccc accgcttcgt gccttgtcct gcgcgcggcc gggcggctgg
     3181 cctgcatcgc cgcgctgctg tcgctcgcgg cctgcgcggc cccgcggccc gaggcgccgg
     3241 cggagtcggc cgcgccgggc gacgagctga tcgtcgagac cgaagcgacc ctcgccgacg
     3301 ccctcgacga cggtcgcgac gaccacgggc acgatgacga cgaccgggcc gaggaccggt
     3361 cgatcgacag cgagacgacc tccgccgcgc ggccggaggc gcacagcgcc gccgaggtcg
     3421 tgcaggcctt cgaagcggtg gacgaacgca tcgaggacct gttcgagcag ctgcgcggcg
     3481 gctacgccct gccggcagtc gacaacgatg ccgtcaggcg acatgccgac tggtacgggc
     3541 gccacccgga ccacctcgag cgcgtgctca accgcgcccg accctacctg ccgcacgtcg
     3601 ccgccgagct gtccgcccgc gggctgccgg ccgagctggc gttcatcccg gtcatcgaga
     3661 gcgccttcga tcccttcgcg tactcgcacg gccgcgcggc cgggctgtgg cagatcgtgc
     3721 ccggcacagg caggcgcttc ggcctgaagc agaactggtg gtacgacggc cgccgtgacg
     3781 tggtcgagtc gacccgtgcc gcactggact acttcgaata cctgcacaag ttcttcgacg
     3841 gagactggct gctggcggta gccgcctaca acagcggcga gggcaccgtg cggcgcgcga
     3901 tccgcagcaa ccaggacgcc gggcggccga ccgatttctg gagcctgcgc ctgccggcgg
     3961 agacgcgcag ctacgtgccg cgcctgctgg ggctggcggc cgtcctggcg gacccggagg
     4021 cgtactcgct gtccttgccg agcgtcgcgc ccacgccggc gttcgccaag gtggagctgg
     4081 acggccagat cgacctggcg gtggcggcgg agctcgcggg catcgagctc gccgacctgc
     4141 aggcgctcaa cccgggtttc aatcgctggg ccaccgatcc cgacggcccg caccggctgc
     4201 tgctgccgcc ggatgcggag gcgtccttcc tggccgcgct ggccgagctg ccgccgaccg
     4261 agcgcgtccg ctggcatcgc caccaggtcg ccgccggcga caccctcggc ggcatcgcgc
     4321 gacggtatcg caccaccgtc cgcgtgctgc agtcggtcaa cggcctcacc ggcaccaaca
     4381 tccgcctcgg cagccacctg atggtgccga cttcgacccg cagcctggac gactatccgc
     4441 tgagcgccgc caaccgcctg gccagcacgc agcaacgcga gcgcagcggg cgggtgcgcg
     4501 ccgagcacac ggtgcgcagc ggcgagtcgc tctggaccat ctcccgcaag cacggcgtcg
     4561 gcgtgcgcga gctggccgcg tggaacggca tggccccggg ggacaccctg gccgcgggcc
     4621 gcaagctggt cgtctggcag cagggcgatg cggcgagcgc ggcgcgcgcc ccggcgagca
     4681 atgccacgct gcgacgggtg cactacaccg tgcgccaggg cgattcgctg taccggatcg
     4741 cgaacaattt ccgcgtgtcg gtcgacgaca tcaagcgctg gaacaagctc gacggcaagc
     4801 gctacctgca gccgggccaa cggctgatcc tgcatgttga cgtcaccgcc cagtcaggcg
     4861 gttgaggaga atcgagatgg catttctgca aggcaaacgc gtgctgatcg tcgggctggc
     4921 gagtgagcgg tccatcgcgt ggggcatcgc acaggcgatg cgccgcgaag gcgccgagct
     4981 ggccttcacc taccagggcg aaaagctcaa gccgcgcgtc gaggacatgg ccgcgcagct
     5041 cggcggcggc ttcacctgcg ccatggacgt tgccgaggac gagcagatca cgggcgcctt
     5101 cgaggcgctc ggccggcact gggacggact cgacgtcatc gtccactcgg tgggcttcgc
     5161 gccgcgcgac cagttgagcg gacgcttcgt cgacgccgtg aaccgcgagg gcttccgcat
     5221 cgcccacgac atcagcgcct acagcttcgc ggcgctcgcc caggcgggcc tgccgctgat
     5281 gcgcggccgc ggcggctcgc tgctgacgct gagctatctc ggcgcggtgc gcgcggtgcc
     5341 caactacaac gtcatgggcc cggccaaggc cagcctggag gccaccgtgc gcttcctggc
     5401 cgcggatctc ggcgccgagg gcctgcgagt caacggcatc tcggcctggc cgatca
//
LOCUS       DAOMPG010000159         5417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6378_length_5417_cov_3.850242, whole genome shotgun sequence.
ACCESSION   DAOMPG010000159 DAOMPG010000000
VERSION     DAOMPG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_5417_cov_3.850242"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..964
                     /locus_tag="PLS34_09000"
     CDS             344..964
                     /locus_tag="PLS34_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRP87643.1"
                     /translation="MDPLIYTLDLTGTFVFALSGGLAAARIRLDPFGFVVIAMVTGLG
                     GGTLRNLLLDLHPVTWVADPLYLGVCIAAALISFAWARWLESRMRWLRIADALGLGLF
                     VVAGTQLALSAGTHGGIAVMMGVTTGIAGGIIRDVLCNEVPLVLQQEIYALAALAGSL
                     SYVLLDAGGADPLLSAALAFTLCAGLRLAAIRWNLSLPAYGGGRMS"
     gene            complement(1339..3669)
                     /locus_tag="PLS34_09005"
     CDS             complement(1339..3669)
                     /locus_tag="PLS34_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRP87644.1"
                     /translation="MRLQTLTILGGLLALLPVLAVPAAAGNAAAEHGVQRMERGALVL
                     ENVPPVPDELAATLFQYQQARAASFAGWLPAPHGMLITTRFGQTRQVHRVADPLGDRQ
                     QLTFFPEAVSNIVTVSDPEQPGFLFGKDSGGDEHYQIYYQDLASGRQSRLTHGGARNT
                     APRWSNMGDYFAYSTTQRNGRDTDIHVYERATGESRPVLQSEGLWYTLDWSPDDRKLL
                     VLRYVSVGESYPHILDLETGELAPFRPSIYPVSFGTARFSRDGRGVFYTSDEDSEFRQ
                     LRFHDLRSGDSRLLTGEIPWDVTSFALSRDGRYLAFVTNADGRSELHLRAVREWRPVA
                     VPDLPMGVVRGLRFSEDGWRIGFTLNSPRTPGDVYSFRVGETQLTRWTKSEVGGLSTD
                     QFVVPELVRYPTFDFADGSRPAEAPGEVAAGPAGDPEGSDAESDPADPKQDSNPDSGE
                     APPPRRTIPAFVYRPAGPGPHPVLVLMHGGPASQARPTFNPVLQYWVNELGLAVIAPN
                     VRGSTGYGREFKMLDDGRLREDSVRDVGALLDWIDTQPELDGARVGIIGGSYGGYMVM
                     ASMIHYGPRLRAGVNVVGISNFVTFLENTGVYRRDRRRLEYGDERDPEMRAYLKSISP
                     TTHADRIVQPLFIAQGANDPRVPQSEADQMVATIRSHGGEVWYFLARDEGHGFRKKVN
                     RDYYNNAVALFLRTHLLDTSPAPGELPAEAPRLEAQDGPAAPVEVPDEDAADEVDDAM
                     HAPLEPPTQDSPEEPLDPVTEPPNGQSTLAQVLQFR"
     gene            complement(3712..4944)
                     /locus_tag="PLS34_09010"
     CDS             complement(3712..4944)
                     /locus_tag="PLS34_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87645.1"
                     /translation="MLVFPAADATGAPAKELPYELRYDLTFRPADDRADIVITLGKGA
                     QHVHWMRFSIKPDRLTDFEADGKLERDGRYLTWTPPDKGGQLRYTAVVSHLRGDRFDA
                     RMTEDWALFRGDDLFPAARLRQSKGAEADATLHVHLPKGWSIATPYEPLGGNAYRIEN
                     PELSFDRPTGWMTAGRIGSRRDEVAGVLVGVAGPLNQGVRRMDMVAFLNWNLGELRAV
                     VDRLPERLLVVSARDKMWRGGLSGPGSLYIHADRPLLSENGTSTLLHELFHVVTRLSG
                     RDGGDWIVEGLAEYYSLKLMWRSGTLSQRRYEAAFEQLASWGGDLDLDELATDRARGR
                     VTARAVLLMRELDREIHRRTGRDKSLDDAVRYLVEAREPVDLERFRAAVEQVMGARSE
                     ILTTGTRFTPIGSMSGVH"
     gene            complement(4976..>5417)
                     /locus_tag="PLS34_09015"
     CDS             complement(4976..>5417)
                     /locus_tag="PLS34_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP87646.1"
                     /translation="AAMLQDEGDTPGGIDFDESDTGYKFFAGYRFDWLPLVSLSAELG
                     YRDLGTPAGSGARYELDGFDYAALAGLGLGPVELFGRLGGMNYDLVKDTGGVLGSFDG
                     TAAVYGVGLRFALLGLGVRAEYEMIDIDELDRVDVVSLSVLYEF"
BASE COUNT          826 a         1876 c         1836 g          879 t
ORIGIN      
        1 gccggtctcg atttccgtgc gcgtgtagac cagttcgccg tcgctggaca cgacgcgttc
       61 cggcatgggc ggcatcttcc ggtagatctg gttgccactc cacagcagca cggcgaacga
      121 gacgaacaac attgccgcaa gaatggtcca gagctttctg gtgctggtca tggcgcagtt
      181 cctcggtgtc gctgaggccg ggtgattgct gcggccctgc ccgaaatgct gcgtccttgc
      241 tgcgactcgg cgcagcgccc ggcccatagg gtagttgctc tcgaccgggc attcaaagat
      301 gatgttggat gctgtgccgc aacataagct ggaacgccga accatggacc cgctcatcta
      361 cacgctcgat ctcaccggca ccttcgtgtt cgcgctctcc ggcgggctgg ccgcggcgcg
      421 catccggctc gatcccttcg gcttcgtggt catcgccatg gtcaccggcc tgggtggcgg
      481 cacgctgcgc aacctgctgc tggatctgca ccccgtgacc tgggtcgcgg atccgctgta
      541 tctcggcgtg tgcatcgcag cggcgttgat cagctttgcc tgggcccgct ggctcgagtc
      601 gcgcatgcgc tggctgcgca tcgccgacgc actggggctc ggcctgttcg tggtcgccgg
      661 cacgcagctc gcgctgagcg ccggcacgca cggcggcatc gcggtgatga tgggtgtcac
      721 cacggggatc gccggcggca tcatccgtga cgtgctgtgc aacgaggtgc cgctggtgct
      781 gcagcaggag atctacgccc tggcggcgct ggccggcagc ctgtcctacg tgctgctgga
      841 tgccggcggc gccgacccgc tgctgtccgc ggcgttggcc ttcaccctgt gcgcggggct
      901 gcgcctcgcc gccattcgct ggaacctgtc gctgccggcc tacggcggcg ggcggatgtc
      961 ctagctcgcc cgctcgagac caggcggagc aacccgcaga gcattgaact gaaaccgatc
     1021 ccggggcaag gcctgggcgc tttctcgatg aagcacatcc ggctccggcc cgccttccca
     1081 tttccgctcg cggcggagtc cgacatgcgg ggcgaggttg ccgacgtctg cctgctcgac
     1141 accggcgact tgttgcggcc cagccacccg gtccgcgact tgccggccgg ggcggaggct
     1201 cagggcaggt aggagtagtc gccgaggtgg ttgacgttgg cccaggccta gatgcggaat
     1261 tccggcccgt agtcggcgcc ccgggacagg cgccgcccca gcccgcatag ctggtgccgc
     1321 gctcgtccag ccggaaactc atctgaactg caggacctgc gccagcgtgc tctgcccgtt
     1381 gggcggctcc gtcaccgggt ccaggggttc ttcgggactg tcctgcgtcg gcggctcgag
     1441 cggcgcgtgc atggcgtcgt cgacttcatc cgcggcatct tcgtccggca cttccacggg
     1501 cgccgccgga ccgtcctgcg cctcgagccg cggagcttcc gctggcaact cgccaggcgc
     1561 cgggctggta tccagcaggt gcgtgcgcag gaacagcgcc acggcattgt tgtaatagtc
     1621 gcggttgacc ttcttgcgaa agccgtgccc ctcgtcgcgc gccaggaaat accagacctc
     1681 tccgccatgg ctgcgaattg tggccaccat ctggtcggcc tccgattgcg gtacgcgggg
     1741 atcgttggcc ccctgggcga tgaacagcgg ctgcacgatg cggtcggcat gggtggtcgg
     1801 cgagatggac ttgagatagg cgcgcatttc cggatcgcgc tcgtcgccgt actccagccg
     1861 gcggcgatcg cgtcggtaca cgccggtgtt ctccaggaag gtcacgaagt tgctgatgcc
     1921 gaccacgttg acgccggccc gcagccgcgg tccgtaatga atcatcgagg ccatcaccat
     1981 gtagcccccg taggagccgc cgatgatgcc cacgcgcgcg ccgtcgagct ccggttgggt
     2041 gtcgatccag tcgagcagcg cgcccacgtc gcggaccgag tcctcgcgca ggcgaccgtc
     2101 gtccagcatc ttgaactcgc ggccgtagcc ggtcgagccg cgcacgttcg gcgcaatgac
     2161 cgccagcccc agctcgttga cccagtactg cagcaccggg ttgaaagtgg gccgcgcctg
     2221 gctcgccggg ccgccgtgca tcaggaccag cacaggatgt ggccccggcc cggcaggccg
     2281 gtagacgaag gcgggaatgg tgcgccgggg cggcggcgct tcgcccgagt cgggattcga
     2341 gtcctgcttc gggtccgcgg ggtccgactc cgcgtccgag ccctccgggt cgcctgcggg
     2401 gccggccgcg acctcgccgg gcgcctcggc cgggcgcgag ccgtcggcga aatcgaaagt
     2461 cggatagcga accagttccg gcacgacgaa ctgatcggtc gacagaccgc cgacctcgct
     2521 cttggtccag cgggtcagct gcgtttcacc gacgcggaag gaatacacgt cgccgggtgt
     2581 gcgcggcgag ttgagcgtga agccgatgcg ccagccgtcc tcggagaagc gcaggccgcg
     2641 caccacgccc atcggcaggt ccggcaccgc caccgggcgc cactcgcgca ccgcccgcag
     2701 gtgcagctcg gagcggccgt cggcattggt gacgaaggcc aggtacctcc cgtcgcggga
     2761 cagcgcgaag ctggtgacat cccaggggat ctcgccggtc aggagccggc tgtcgccgct
     2821 gcgcaggtca tggaaccgca attgccgaaa ctcgctgtcc tcgtccgagg tgtagaacac
     2881 gccgcggccg tcgcgtgaaa atcgggcggt tccgaaggat accgggtaga tgctcggccg
     2941 gaacggcgcc agctcgccgg tctcgagatc gagaatgtgc ggataggatt cacccaccga
     3001 cacgtagcgc agcaccagca gcttgcgatc gtccggcgac cagtccagcg tgtaccacaa
     3061 gccctcactc tgcagcaccg ggcggctttc gccggtggcg cgctcgtaga cgtggatgtc
     3121 cgtgtcccgg ccattgcgct gggtggtcga ataagcgaag taatcgccca tgttggacca
     3181 acgcggcgcc gtattgcgcg cgccgccgtg cgtcaaacgg gactgccggc cgctggccag
     3241 gtcctggtag tagatctggt agtgctcgtc gccgccggag tccttgccga acaggaagcc
     3301 tggctgctcc gggtcggaca cggtgacgat gttgctgacc gcctcgggga agaaggtcag
     3361 ttgctggcgg tcaccgagcg gatcggcgac ccggtgcacc tgccgcgtct gtccgaagcg
     3421 ggtggtaatc agcatgccgt gcggggccgg cagccagccg gcgaaggacg ccgcccgcgc
     3481 ctgctggtac tggaacaggg tcgcggctag ctcgtccggg acgggcggga cgttctccag
     3541 caccagggcg ccccgctcca tgcgctgcac gccgtgctcc gcggcggcat tcccggccgc
     3601 cgccgggacc gcgagcactg gcaggagggc cagcagcccg ccaaggattg tcagtgtctg
     3661 caatcgcatg ggtcgtgaga ctccccgcgc ggggaacagt ttcaagtccg gctagtgaac
     3721 accagacatc gacccgattg gcgtgaaccg ggttccagtt gtgaggattt ccgaccgcgc
     3781 ccccatgacc tgctccactg cggcccggaa ccgctcgaga tcgaccggct cacgcgcctc
     3841 gaccaggtac ctcacggcgt catcgaggct cttgtcgcgc ccggtacgcc ggtgaatctc
     3901 gcgatcgagc tcccgcatca gcaacaccgc gcgcgccgta acccggccgc gcgcccggtc
     3961 ggtcgccagc tcgtcgaggt ccaggtcgcc gccccagctc gccagctgct cgaaggccgc
     4021 ctcgtagcgc cgctggctga gcgtgcccga gcgccacatg agtttcagcg agtaatactc
     4081 cgccagcccc tcgacgatcc agtcgccgcc gtcccgcccg ctgaggcgcg tcacgacgtg
     4141 aaaaagctcg tgcagcagcg tgctggtgcc gttttcgctc agcagcggcc ggtccgcgtg
     4201 gatgtacagg gagcccggtc cggagaggcc gccacgccac atcttgtcgc gcgcgctgac
     4261 caccagcaga cgctccggca gccggtccac gacggcgcgc aattcgccga ggttccagtt
     4321 caggaaggcg accatgtcca tcctgcggac gccctggttc aggggacccg cgacgccgac
     4381 cagcaccccg gcgacctcgt cccgccgcga gccgatccgc cccgccgtca tccagcccgt
     4441 gggacggtcg aagctgagct ccgggttctc gatgcgataa gcgttgccgc ccagcggctc
     4501 gtagggcgta gcgatggacc aacccttcgg gaggtgcaca tgcagggtgg catcggcctc
     4561 cgcgcccttg ctctgccgca gccgcgcggc ggggaacagg tcgtcgccgc gaaacagcgc
     4621 ccagtcctcg gtcatgcggg catcgaagcg atcgccgcgc aggtggctga ccacggccgt
     4681 gtaccgcaat tgcccgccct tgtccggcgg cgtccaggtc aggtagcggc cgtcgcgctc
     4741 gagcttgccg tcggcctcga agtccgtcag ccggtccggc ttgatggaga agcgcatcca
     4801 gtggacgtgt tgtgcgccct tgccaagcgt gatgacgatg tcggcccggt cgtctgcagg
     4861 tcggaacgtc aggtcgtagc gcagctcgta gggcagctcc ttggctggag ctccggttgc
     4921 atctgccgcc gggaagacca gcacgcacgc tgccgcgcac caccaccggg caaacctaga
     4981 actcatagag cacgctcaat gacaccacgt ccacgcggtc cagttcgtcg atgtcgatca
     5041 tctcgtactc ggcccgcacg ccgagaccaa ggagcgcgaa ccgcaggccg acgccgtata
     5101 cggccgcggt gccgtcgaag ctgccgagca cgccaccggt gtccttgacc aggtcatagt
     5161 tcatcccgcc cagtcgcccg aacaattcca ccgggccgag accgagtccc gccagcgcgg
     5221 catagtcgaa gccgtcgagc tcgtaccgcg cgcccgagcc ggccggggta ccgagatcgc
     5281 gatagccgag ttcggcggac aggctgacca ggggcagcca gtcgaagcga tagccggcga
     5341 agaacttgta gccggtgtcg ctttcgtcaa agtcgatacc gcccggcgtg tcgccctcgt
     5401 cctggagcat ggcggca
//
LOCUS       DAOMPG010000160         5394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6411_length_5394_cov_4.716052, whole genome shotgun sequence.
ACCESSION   DAOMPG010000160 DAOMPG010000000
VERSION     DAOMPG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6411_length_5394_cov_4.716052"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="PLS34_09020"
     CDS             <1..2143
                     /locus_tag="PLS34_09020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRP87647.1"
                     /translation="TPWLATEYIEGEPITAYCTARNLGVEARLDLFLQVADAVHHAHQ
                     KLVVHRDLKPSNILVDAQGRVRLLDFGIAKLLDPDSATGPAALTRAAHRPMTPEYAAP
                     EQLAGGAITTATDVFQLGVLLYELLAGQRPPRSAGNTAHGETPPPSTAALRDTGAPYR
                     TVALPPNSAAQLARRLKGDLDLIVLKALQEDPERRYLSVAGLADDVRNHLKGRAISAR
                     PESALDATRRFFRRRPWAAAALALALGLVATLQVSAVRLGHERDAAQREAARVTQVKD
                     LLVNLFRQADPMAEDALRGRETTIWDSVQAATARVRDELHHDPALRAEMLGTLAMLHH
                     YAGHMEEGAELLEEVVALHREQEGPRSAPYAVALAELARHRLKLDRRADAMAAIEEAL
                     ALGRGLPNDAVAARVAILLDAGEVLRAYGSLHDVETVFREAVALAGVAASLPLASRLA
                     ATNGLAEVLSELGDDREAEALALASIAAVENELGVGHARLVAPLSILGSSQNALGRPR
                     DAVASLERGLRIIEREYGDRYESTYNMRNNLVLALAAAGEHERAVGEMLVLLEQRRAA
                     LGDGHLEVANSMQNLAVMLVLAGNPGEALAILEQVRASYAASLPPDHYRLAFPLLTQS
                     FIRLRQDEPELAELAARPALRILQGALPATHYAVGIAGCLLGEAELALGRREEAAARL
                     AEALPIADAGQANMLPYVAHCREAHAAATSQ"
     gene            complement(2133..3452)
                     /gene="rimO"
                     /locus_tag="PLS34_09025"
     CDS             complement(2133..3452)
                     /gene="rimO"
                     /locus_tag="PLS34_09025"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRP87648.1"
                     /translation="MNRRIGFVSLGCPKALVDSERILTRLRAEGYETVPTYEDADLVV
                     VNTCGFIDEAVEESLEAIGDAMAANGKVLVTGCLGARPEAIREKHPGVLGITGPHAYE
                     QVMAAVHEHLPPAHDPFTDLVPPQGIRLTPRHYAYLKISEGCNNKCSFCIIPDLRGKL
                     ASRPIGEVLREAERLVGAGVRELLVISQDTSAYGADIRYLPDRWRGERYETRFIDLAQ
                     ALGELGAWVRMHYVYPYPHVDKVLPLMAEGKVLPYLDIPFQHASPAVLKAMRRPAAAV
                     DTLARIRRWRETCPELTLRSTFIVGFPGETEQDFERLLEWLEEAQLDRVGCFRYSPVA
                     GAAANGLGAPVPDEIKEERWQRFMETAARISAARLDARVGAIEPVIVDEIDVEQDIAL
                     ARSRGDAPEIDGRVIVAGGGGLRQGDIVEVEITAADEYDLEGQLVTG"
     gene            3623..3823
                     /gene="thiS"
                     /locus_tag="PLS34_09030"
     CDS             3623..3823
                     /gene="thiS"
                     /locus_tag="PLS34_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRP87649.1"
                     /translation="MEIILNGEPRDVPEGLSAAGLVELLGLAGRRYAMEVNGELLPKS
                     AHVTHALGPGDRIEIVQAVGGG"
     gene            3825..4640
                     /locus_tag="PLS34_09035"
     CDS             3825..4640
                     /locus_tag="PLS34_09035"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRP87650.1"
                     /translation="METASKQDTARGQDDFLELAGRKYRSRLLTGTGKYRDFDETLAA
                     TEASGAEIVTVAIRRTNIGQDKTQPNLLDYLSPERFTILPNTAGCFTVQDAVRTCRLA
                     RELLDGHPLVKLEVLADQKTLFPDITATLEATEILVNEGFQVMVYTNDDPIIAKRLED
                     MGCISVMPLAAPIGSGLGIRNPYNILTIVENAKVPILVDAGVGTASDAAIAMELGCDG
                     VLMNTAIAGARNPVLMASAMRKAVEAGREAFLAGRIPRKRFASASSPLDGTFF"
     gene            4643..5386
                     /gene="trmB"
                     /locus_tag="PLS34_09040"
     CDS             4643..5386
                     /gene="trmB"
                     /locus_tag="PLS34_09040"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRP87651.1"
                     /translation="MAGQERDEPPVHLRRIRSFVRRAGRLTPSQARALEELWPRFGID
                     PPAGPLDLEATFGRLAPRTLEIGFGNGDNLAELAARHPERDYLGVEVHDPGVGHLLLR
                     IEREGLANIRIVRHDAVEVVTGWLPPDSIDEALIFFPDPWHKKRHHKRRLVQSEFLAQ
                     LARVMPPGARLHLATDWAPYAEQMLEVCEASPLFENAAPGGGYAARPATRPVTRFERR
                     GLRLGHEVFDLLYRRKAGSETGSSTPAPR"
BASE COUNT          847 a         1897 c         1843 g          807 t
ORIGIN      
        1 cacgccctgg ctggcgacgg agtacatcga gggcgagccc atcactgcgt actgcacggc
       61 acgcaacctc ggcgtcgagg cccgcctcga cctgttcctg caggtggccg acgccgtgca
      121 ccatgcccac cagaagctgg tggtacatcg cgacctgaag ccgagcaaca tcctggtgga
      181 cgcgcagggc cgcgtgcgcc tgctcgactt cggcatcgcc aagctgctcg accccgactc
      241 cgccaccggc ccggccgcgc tcactcgtgc cgcgcaccga ccgatgacgc cggagtacgc
      301 cgccccggag cagctggccg gcggcgccat caccaccgcc acggacgtgt tccagctcgg
      361 cgtgctgctc tacgagctgc tcgccgggca acgcccgccg cggtcggccg gcaacaccgc
      421 ccacggcgaa acaccgccgc ccagcacagc cgcgttgcgg gacaccggcg cgccctaccg
      481 tactgtcgcg ctcccgccca actcagccgc gcaactcgcc aggcgactga agggcgacct
      541 cgacctcatc gtgctgaaag cgctgcagga agatcccgag cgtcgatacc tgtcagtggc
      601 gggactcgct gacgacgtcc gtaaccatct caagggccgc gccatttccg cacgcccgga
      661 atcggcgctg gacgcgaccc gccggttttt tcgacgccgg ccatgggcgg cggcggccct
      721 ggcgctggcg ctaggcctgg tggcgacgct gcaggtgtcg gcggtgcgac tggggcacga
      781 acgagacgca gcgcagcgtg aagctgcccg ggtcacacag gtgaaggacc tgctcgtgaa
      841 cctgttccgc caggcggatc ccatggcgga agacgcgctg cgcgggcgcg aaaccaccat
      901 ctgggactcg gtgcaagccg cgaccgccag ggtgcgcgac gaactgcacc acgaccccgc
      961 gctgcgcgcc gagatgctgg gtacgctggc catgctgcat cactacgcgg ggcacatgga
     1021 ggaaggcgcg gagttgctcg aggaagtcgt tgcactgcat cgcgagcagg aaggacccag
     1081 gtcggcgccc tatgccgtcg cgcttgccga actggcgcgt caccggttga agctggatcg
     1141 ccgcgcggac gcgatggccg ccatcgaaga ggccctcgcg ctcgggcggg gcctgccgaa
     1201 tgatgcggtg gcggcacgcg tggcgatcct gctcgatgca ggcgaagtgc tgcgggcata
     1261 cggttcgctg catgacgtcg agactgtctt tcgcgaggcc gtggcgctgg ccggcgtggc
     1321 ggcctcgttg ccgctggcaa gtcgcctcgc cgccaccaac ggcctggccg aggtcctgag
     1381 cgaactgggg gacgaccgcg aggcggaagc gctggcgctg gcctcgattg ccgccgtgga
     1441 aaacgaactg ggagtcgggc acgcaaggct cgtggcgccg ctgtcgatcc tcggctcctc
     1501 ccagaacgcc ctggggcgcc cgcgcgatgc cgtcgcctcg ctggagcgcg gcctgcgcat
     1561 catcgagcgc gagtacggcg accgttacga gtcgacctac aatatgcgca acaacctggt
     1621 gctcgcgctc gcggccgcag gcgagcacga acgcgcggtg ggcgagatgc ttgtcctgct
     1681 cgagcagcgc cgcgctgcgc ttggcgacgg ccatctcgag gttgccaatt ccatgcagaa
     1741 cctggccgtg atgctggtgc tggccggcaa tcccggcgaa gcgctggcga ttctcgagca
     1801 ggtgcgcgcc agctatgcgg cgtcgctgcc gccggaccat taccgcctgg cctttccgtt
     1861 gctgacccag tccttcatcc gcctgcggca ggacgagccc gaactggcgg agcttgccgc
     1921 acggccggcg ctgcgaatcc tgcaaggtgc gctgcccgca acgcactacg cggtcggcat
     1981 cgccggctgc ctgctcggcg aggccgaact cgcgctgggg cggcgtgaag aagctgcggc
     2041 acgcctggcg gaggccctgc cgatcgccga cgccggacag gccaacatgc ttccctacgt
     2101 cgcgcattgc cgggaggcgc acgccgccgc gactagccag tgaccagttg cccctcgagg
     2161 tcgtactcgt ccgccgctgt aatctcgacc tcgacgatgt cgccctgcct gaggccaccg
     2221 ccgcccgcca cgatcacccg cccgtcgatt tccggcgcat cgccgcggct gcgcgccagc
     2281 gcgatgtcct gctccacatc gatctcgtcc acgatcaccg gctcgatggc gccgacgcgc
     2341 gcgtccagcc gcgctgcgct gatccgcgcc gctgtctcca tgaagcgctg ccagcgctct
     2401 tccttgatct cgtccggaac gggtgcgccg agcccgttgg cggccgcgcc tgccaccggc
     2461 gaataacgga aacagccgac gcggtcgagc tgcgcctcct ccagccattc cagcaaccgc
     2521 tcgaagtcct gctccgtctc accggggaag ccgacgatga aggtgctgcg gagtgtcagc
     2581 tcggggcagg tctcgcgcca gcgccggatg cgtgccagcg tgtcgaccgc cgccgcgggg
     2641 cgccgcatgg ccttcaacac ggcgggactc gcgtgctgga agggaatgtc caggtatggc
     2701 agcaccttgc cctcggccat cagcggcagc accttgtcga catgcggata gggatagacg
     2761 tagtgcatgc gcacccaggc gcccagctcg ccgagcgcct gcgccaggtc gatgaagcgg
     2821 gtctcgtagc gctcgccgcg ccagcggtcg ggcaggtagc ggatgtcggc gccgtaggca
     2881 ctggtgtcct gggagatcac cagcagctca cgcaccccgg cgccgaccag ccgctcggcc
     2941 tcgcgcagca cctcgccgat cggccggctg gcgagcttgc cgcgcaggtc ggggatgatg
     3001 cagaagctgc acttgttgtt gcagccctcg gaaatcttca ggtaggcgta gtggcgcggc
     3061 gtcaggcgga tgccctgcgg cggcacgagg tcggtgaagg ggtcatgcgc cgggggaaga
     3121 tgttcgtgca cggcggccat gacctgctcg taggcgtgcg gaccggtgat gccgagcacc
     3181 cccgggtgct tctcgcgaat ggcctccggc ctggcgccga ggcagccggt gaccagcacc
     3241 ttgccattgg cggccatggc gtcgccgatc gcctcgagcg actcctcgac ggcctcatcg
     3301 atgaagccgc aggtgttcac caccacgagg tcggcgtcct cgtaggtggg cacggtctcg
     3361 tagccctccg cgcgcagccg ggtgaggatg cgctcggagt ccacgagcgc cttggggcag
     3421 ccgaggctga caaagccgat gcgacggttc atggcagggt atgttatcgg caaatgggga
     3481 cagaaaaact ggggtcagag acaggtttcg cgtgcgaaac ctgtctctga ccccagtttt
     3541 tctgaaaagg ggaatgtccc cgatttcgcc atgtagaata ctgccctgag ttccccaaga
     3601 cccaggccgg cctgaccccc gcatggaaat catcctgaac ggcgagcctc gcgatgtgcc
     3661 cgagggcctg agcgccgccg ggctggtgga gctgctcggc ctggccggcc ggcgctatgc
     3721 catggaggtc aacggcgaac tgctgccgaa aagtgcgcat gtgacccatg cgctgggtcc
     3781 cggggaccgt atcgagatcg tgcaggccgt gggaggcgga tgacgtggaa acagccagca
     3841 agcaggacac cgcccgcggg caggacgatt tcctcgagct ggccgggcgg aagtaccgct
     3901 cgcgcctgct caccggcacc ggcaagtacc gcgacttcga cgagacgctt gcggccaccg
     3961 aggccagcgg cgcggagatc gtcacggtcg ccatccgccg caccaatatc ggccaggaca
     4021 agacccagcc caacctgctc gattacctgt cgccggagcg cttcaccatc ctgccgaaca
     4081 ccgccggctg cttcaccgtg caggacgcgg tgcgcacctg ccggctggcg cgcgaactgc
     4141 tcgacggcca cccgctggtg aagctggagg tgctggccga ccagaagacg ttgttcccgg
     4201 acatcacagc gacgctcgag gccacggaaa tcctcgtcaa cgagggcttc caggtgatgg
     4261 tctacaccaa cgacgacccg atcattgcca agcgcctcga ggacatgggc tgcatctcgg
     4321 tcatgccgct ggctgcgccc atcggctccg ggctgggcat ccgcaatccc tacaacattc
     4381 tcaccatcgt cgagaacgcg aaggtgccga tcctggtgga cgccggcgtc ggcaccgcgt
     4441 cggacgcggc gatcgccatg gagctcggct gcgacggcgt gctgatgaac accgcgatcg
     4501 ccggcgcgcg caacccggtg ctgatggcct cggcgatgcg caaggcggtc gaggcgggcc
     4561 gcgaggcgtt tctcgcgggc cgcatcccgc gcaagcgctt cgcctccgcg tcgtcgccgc
     4621 tcgacgggac tttcttctga gcgtggcggg acaggagcgc gacgagccac ccgtccatct
     4681 tcgccggatc cgcagcttcg tccggcgcgc ggggcggctc acgccgtccc aggcccgtgc
     4741 actcgaggaa ctctggccgc gcttcggtat cgacccgccg gcggggccgc tcgacctgga
     4801 agcgaccttc ggccgcctgg ccccgcgcac cctggagatt ggcttcggca acggcgacaa
     4861 cctcgcggag ctggccgcac gccaccctga gcgcgactac ctgggcgtgg aggtgcacga
     4921 cccgggcgtc ggccacctgc tgctgcggat cgagcgtgag ggcctcgcga acatccgcat
     4981 cgtgcgccac gacgcggtcg aggtcgtgac cggctggctg ccgccggact ccattgacga
     5041 ggcgctgatc ttttttccgg acccctggca caagaagcgc caccacaagc ggcgtctcgt
     5101 gcagtcggag ttcctcgccc agctggcgcg cgtcatgccg cccggggcac ggctgcatct
     5161 cgccacggac tgggccccct acgccgagca gatgctggaa gtgtgcgagg cctcgccgtt
     5221 gttcgagaac gccgcgcccg gcggcggtta cgcggcacgg ccggcgacgc ggccggtgac
     5281 ccggttcgag cgccgcggat tgcggctcgg gcacgaagtc ttcgacctgc tgtaccggag
     5341 aaaggcaggg tcggagacag gttcttctac gccagcacct cggtgacgcc gtcc
//
LOCUS       DAOMPG010000161         5383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6431_length_5383_cov_4.509384, whole genome shotgun sequence.
ACCESSION   DAOMPG010000161 DAOMPG010000000
VERSION     DAOMPG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_5383_cov_4.509384"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLS34_09045"
     CDS             <1..364
                     /locus_tag="PLS34_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRP87652.1"
                     /translation="MDFLRHVVASREHYLFELWLMLGGVLAAPVFASSVITLPLLLDR
                     QVNVLQAVTASWLVVLRNPVVMACWAALIAGLSLAGIGLFLVGSLVVVPVLGHASWHA
                     YTDTLDAAVLPERVRGAP"
     gene            376..585
                     /locus_tag="PLS34_09050"
     CDS             376..585
                     /locus_tag="PLS34_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HRP87653.1"
                     /translation="MFGFTEQQISWFGLTFGLAAFMLYMLFIIGQIAYQSKAGLFGTF
                     VLFLVLALGILGFVAKAVIQWVLGI"
     gene            complement(590..1012)
                     /locus_tag="PLS34_09055"
     CDS             complement(590..1012)
                     /locus_tag="PLS34_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP87654.1"
                     /translation="MIVLDTNVLSELMRPAPDAGVVAWLGARDPFELATTTITIAEIQ
                     RGIVRLPDGRRRKSLEQRFSAFVEAAFPGRLLAFDRDAAYACGEVSASRERNGLHADA
                     VDMMIAAIVKTAGATLATRNMRDFEACGIALADPWRSY"
     gene            complement(1014..1259)
                     /locus_tag="PLS34_09060"
     CDS             complement(1014..1259)
                     /locus_tag="PLS34_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HRP87655.1"
                     /translation="MANINVRGLSDKTKEALRVRAAKAGMSLEAYARRALTKASTEKA
                     EEPKPVVELAETYFGTRHGVELDIPPRATSRDPPEFP"
     gene            complement(1343..1921)
                     /locus_tag="PLS34_09065"
     CDS             complement(1343..1921)
                     /locus_tag="PLS34_09065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP87656.1"
                     /translation="MSRAGRESPLVVETLARSHDRAGFSSGCGPLDEYLQRRALQDQR
                     RRAAVCHVLVSPAEPARIIGYYTLSSYTIRLAELPAALAGKLPRYPDVPCALLGRLAV
                     DTAFRGRRLGEFLLVDAMYRCFTRVASQVAVYALVTHAKDDAAAAFYRRYDFIPFPAT
                     PLTLFLPMATISDLFVRQARRPARALQVPAGR"
     gene            complement(1931..2215)
                     /locus_tag="PLS34_09070"
     CDS             complement(1931..2215)
                     /locus_tag="PLS34_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRP87657.1"
                     /translation="MQARQLKSERFEARLRPEDKALMQRAADLSGRKLSEYVIISARE
                     AAVRTVRQYEGMVLSEPRDQEAFAAALLQPPPPGRRLKAAARRYRSMSRP"
     gene            2540..2788
                     /locus_tag="PLS34_09075"
     CDS             2540..2788
                     /locus_tag="PLS34_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017028225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRP87658.1"
                     /translation="MPHQLLAETAASISELKANPMKVVASGKGMPVVVLNRNEPAFYC
                     VPAGAYEAMMELIEDLELLELVKQREQEESVRVSVDDL"
     gene            2778..3074
                     /locus_tag="PLS34_09080"
     CDS             2778..3074
                     /locus_tag="PLS34_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRP87659.1"
                     /translation="MTYELAFKTSALKEWRKLGHAVRAQFRKKLIERLQSPHVPSAAL
                     SGAVNIYKIKLRRAGYRLVYSVEDRTVTVTVIAVGKRGRNEVYDLALSRLKDPG"
     gene            3454..3813
                     /locus_tag="PLS34_09085"
     CDS             3454..3813
                     /locus_tag="PLS34_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRP87660.1"
                     /translation="MAEKRKVLYWEGSAKKDFKEFPVPVQKDMGVALFVVQLGQTPES
                     AKLWKGLGSGVYELTEDHRGDAFRVVYAVRVGNAVHVLHAFRKKSKSGIATPQRDVEL
                     IERRLKKVLALGASGRS"
     gene            3815..4135
                     /locus_tag="PLS34_09090"
     CDS             3815..4135
                     /locus_tag="PLS34_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP87661.1"
                     /translation="MTRKGQPHGIDNVLVDLGFEDAEELSAKAALAVKLNELIDRRGL
                     SQTEAAHITGMTQPKISRVKRYKLQNISLERLMQALVSLDQQVEIVVRPARRSHAGGI
                     TVAA"
     gene            4190..4663
                     /locus_tag="PLS34_09095"
     CDS             4190..4663
                     /locus_tag="PLS34_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRP87662.1"
                     /translation="MKAIPLAALAALLMLAGGAGAEDEAAAVSPAFDEALAASVGADD
                     NGMRQYVLVILKTGPTPVPAGEARSEMFRGHFANMKRLAAEGKLALAGPLDGVDGWRG
                     LFILAVSDLEEAAALVATDPVIINGEMVAEYHKYYGSAALMLVNDAHAKVARKGF"
     gene            complement(4693..4917)
                     /locus_tag="PLS34_09100"
     CDS             complement(4693..4917)
                     /locus_tag="PLS34_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRP87663.1"
                     /translation="MTQVSPHGVWLLVRGEELLMPFVDFPWFQGASVDALSHIEEPVQ
                     GHFYWPDLDVDLGLETIRHPERFPLKSRVR"
     gene            complement(4919..4996)
                     /locus_tag="PLS34_09105"
     CDS             complement(4919..4996)
                     /locus_tag="PLS34_09105"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87664.1"
                     /translation="MKTCEQIIEEHRDELIAAWHKHFSG"
     gene            5137..>5383
                     /locus_tag="PLS34_09110"
     CDS             5137..>5383
                     /locus_tag="PLS34_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRP87665.1"
                     /translation="MPHENDRELNPEGFARQARAAELEGEIASLAAHINAATYRLIEM
                     LREFDELRGWSGWPTPAHWLQYRCGYSLGAARERFRVA"
BASE COUNT          956 a         1711 c         1764 g          952 t
ORIGIN      
        1 gatggatttc ctgcgccacg tggtcgcgtc gcgggagcac tacctgttcg agctgtggct
       61 gatgctcggc ggcgtgctgg ccgcgccggt gttcgcctcg agcgtcatca ccctgccgct
      121 gctgctggat cgccaggtga acgtgctcca ggcggtgacc gcgagctggc tggtggtgct
      181 caggaacccg gttgtgatgg cgtgctgggc ggcgttgatc gcggggctgt cgctggcggg
      241 aatcggcctg ttcctggtgg gcagcctggt ggtggtgccg gtgctgggcc acgccagctg
      301 gcatgcctac acggacacgc tggacgccgc cgtgctgccg gagcgggtgc ggggcgcgcc
      361 gtgaacgccg ccgacctgtt cggcttcacg gagcagcaga tctcctggtt cggcctgacc
      421 ttcgggctgg cggccttcat gctctacatg ctgttcatca tcggccagat cgcctaccag
      481 tcgaaggccg ggctgttcgg caccttcgtg ctgttcctgg tgctcgcgct cggcatcctc
      541 ggcttcgtgg cgaaggcggt gatccagtgg gtgctgggga tttgactggt cagtaactcc
      601 gccaggggtc ggcaagcgca atcccgcacg cctcgaaatc gcgcatgttc cgcgtggcca
      661 gcgttgcccc ggcagtcttc acgatcgccg caatcatcat gtcgaccgcg tccgcatgaa
      721 ggccgtttcg ctcccgtgac gccgagacct cgccacacgc gtaggccgca tcccggtcga
      781 aagcgagcag ccggcctggg aaggccgctt cgacaaaggc ggaaaatcgc tgctccaggc
      841 tcttgcgtcg ccggccgtcg ggcaggcgaa cgatgccgcg ctggatctcg gcgatggtga
      901 tggtggtcgt ggccagctcg aacgggtcgc gcgcgccgag ccacgccacg acccccgcgt
      961 cgggtgccgg ccgcatcagc tccgagagaa cgttagtgtc aagaacgatc acctcaggga
     1021 aactctggcg ggtctcgact cgtagcgcgt ggcgggatgt cgagctcgac tccatgccgg
     1081 gtgccgaaat atgtctccgc cagctcgacc accggcttcg gctcttcggc cttttcggta
     1141 gaagccttgg tcagcgcgcg acgcgcataa gcctcgagcg acatgcccgc cttcgcggcc
     1201 cgcactcgca gcgcttcctt ggtcttgtcg gaaaggcctc ggacgttgat gttggccatg
     1261 gtgccctccg gaacggcgca aatctgacgc cattgtatcc attgagtcca ttggagtcac
     1321 tgctgcagac tccacagcga gatcatcgcc cggctgggac ctgcagtgca cgcgccgggc
     1381 ggcgcgcctg acgcacgaac aggtccgaaa tggtagccat cggcaggaac agggtcagcg
     1441 gcgtggccgg gaacgggatg aagtcgtagc gccggtagaa ggccgcggcg gcatcgtcct
     1501 tggcgtgggt caccagcgcg tagacggcca cctggctggc gacccgcgta aaacaccggt
     1561 acatggcgtc gaccagcagg aactcgccca ggcgccggcc gcgaaaagcg gtgtcgacgg
     1621 cgagacggcc cagcaacgcg cacggcacat cgggatagcg cggcagcttc ccggccaacg
     1681 cggcaggaag ttcggcgagg cggatcgtgt agctggagag cgtgtagtag ccgattatgc
     1741 gcgccggctc agccggcgag accagcacgt gacagacggc cgcgcggcgg cgctggtcct
     1801 gcagcgcacg ccgttgcaga tactcgtcga gcggcccgca gccgctgctg aagccggccc
     1861 ggtcatgcga ccgggcgagt gtctccacga ccagcggaga ctcgcgcccc gcgcgggaca
     1921 ccggccgccc tcaaggccgg gacatcgaac gataacgccg cgcagcggcc ttgagccggc
     1981 ggcccggcgg cggcggttgc agcaacgccg cggcgaatgc ctcctggtcg cgcggctcgc
     2041 tcagcaccat gccctcgtac tgtcggaccg tccgcacggc ggcctcgcgg gcgctgatga
     2101 tgacgtactc gctgagcttc cgccccgaga gatccgcggc gcgctgcata agcgccttgt
     2161 cctctggccg caggcgcgcc tcgaatcgct cgctcttgag ctgcctggct tgcatgcttg
     2221 tttttccttt catgggccca gactgtacgg cagatcgccg tacatcgtaa agcgctccac
     2281 caggccgcat gatctggcgg tgcacgggca ttcacaatat ggcgtttctg ggtggaccct
     2341 tcggaaacgc cctcactccc cctcgagcat tctcctcagc cgccgcgagg cgctagagaa
     2401 ctttaccttc gcgggttcga agaaaaatat ccctgcaccc ctgtttcggt gaacctggat
     2461 ggaagaggcg gcgaacttgt caaagacctt taaaaagacc tattattcgg tctcttcaga
     2521 ctctccggaa gagaccagca tgccccacca actccttgcc gagacggctg caagcatctc
     2581 cgagctcaag gcgaacccca tgaaagtggt ggccagcggc aagggcatgc ccgtcgtcgt
     2641 ccttaaccgc aacgagccgg ccttctattg cgttccggcc ggtgcctacg aggcaatgat
     2701 ggagctgatc gaggacctcg agctgctcga gctggtcaag cagcgcgagc aggaggagtc
     2761 ggtccgggtt tcggtcgatg acctatgagc tggcgttcaa gacctccgcg ctgaaggaat
     2821 ggagaaagct cggacacgct gtccgggccc agttcaggaa aaaactgatc gagcgacttc
     2881 agagcccgca tgtgccgagc gctgcgctgt ccggcgcggt gaacatttac aagatcaagc
     2941 tgcgccgcgc cggctaccgg ctcgtttact ccgtcgagga ccgcaccgtc accgtgaccg
     3001 tgattgcggt cggcaagcgc ggccggaacg aggtgtacga ccttgccctg tcccggctga
     3061 aggatccggg gtgagatatc ttcacgcagc ggtcaattgg caactgacct gacgtctccg
     3121 cggagacgcc tcaccccggc cctttcaccc cgtaccccgc cttcgccgcg atccccacca
     3181 tcgccttgac cgcgctctcc agcttgcccg ccagccgcgc gagccggccc ggggtcagtc
     3241 cggggcggac attgccagtt tagacgctgg agggctcagg gggtgagcca tgcggcgtcg
     3301 ggaccggctg gaaaatcgcg ttagaaccga tgcttttcgt cagttgaatt gagctgctcg
     3361 gacagtactt tccagagagt gctgaactgg gacgctttcc ggatgacccg ctacctggat
     3421 tgccttgcct tttatacctg aacggatata gtaatggccg agaaacgcaa ggtcttgtac
     3481 tgggagggct ccgcgaagaa ggacttcaag gaattcccgg tccctgtcca gaaggatatg
     3541 ggtgtcgctt tgttcgtcgt gcagttgggc cagacgcccg aatcggccaa actctggaaa
     3601 gggctcggtt caggagtcta tgaactgaca gaagaccacc gcggtgacgc tttccgcgtg
     3661 gtttacgccg tgcgcgtcgg gaacgcagta catgtgctgc atgcgtttcg taagaagtcc
     3721 aagtccggca ttgccacacc gcagcgggac gtggaattga tcgaacggcg gttgaagaaa
     3781 gtgcttgccc ttggggccag cgggagatct tgagatgaca cgcaaaggcc aacctcacgg
     3841 cattgacaac gtgctggtag acctcgggtt cgaagatgcg gaagagctgt cggcgaaggc
     3901 cgccctcgcg gtcaaactga atgaattgat cgaccggcgc ggtctgagtc agaccgaggc
     3961 ggcccacatc accggcatga cgcagccgaa gatatcgcgg gtcaagcgct acaagctgca
     4021 gaacatttct cttgaacgct tgatgcaggc gttggtgtcg ctcgatcaac aggtagaaat
     4081 tgtcgtgcgg ccggcgcgcc gctcgcatgc aggcggaatt acggttgccg cttagcgccg
     4141 cgccgtgtcg taggattgaa cgctatcacc ccgacccgga gctcatgcca tgaaagcgat
     4201 ccctctcgcg gcgctggccg cgctgttgat gctggccggc ggcgcgggcg ccgaggacga
     4261 agccgccgcg gtgagcccgg ccttcgacga ggcgctggcc gcttcggtcg gcgcggatga
     4321 caacggcatg cggcagtacg tgctggtcat cctcaagacc ggcccgacgc cggtgcctgc
     4381 gggcgaggca cgcagcgaaa tgttccgcgg ccacttcgcc aacatgaagc gcctcgcggc
     4441 ggaagggaaa ctggcgctgg cgggcccgct cgacggcgtg gacggctggc gcggcctgtt
     4501 catactggcg gtctccgatc tcgaagaggc cgcggcgctg gtcgccaccg acccggtcat
     4561 catcaacggc gaaatggtcg ccgagtatca caagtactac ggctcggcgg cgctgatgct
     4621 cgtcaacgac gcccacgcga aggtggcgcg gaaggggttc tagcggagct gtaggccgct
     4681 gccgagcgag ggctagcgca cccggctctt gagcgggaag cgctccgggt gccggatggt
     4741 ctccaggccg agatcgacat ccaggtccgg ccagtagaag tgcccttgca caggctcctc
     4801 gatgtgcgat agcgcgtcga ccgatgcgcc ctggaaccac gggaagtcaa caaagggcat
     4861 taacagctct tcgccacgca ccagcagcca cacgccatgc ggcgagacct gggtcacctc
     4921 aaccgctgaa gtgcttgtgc catgcggcga tgagctcatc gcggtgctcc tcaatgattt
     4981 gctcgcacgt tttcaattgt gctcgcgata acccgaaatt cctagcgagc tcaatgcgtc
     5041 tattgccctc ggaagaacta tagaaaaaac cgattgcaat tcaacgacat ccgggtaaca
     5101 atactgtatg ttttgacagt gcccgggagc gccggaatgc cccacgaaaa cgaccgcgag
     5161 ctgaaccccg agggcttcgc ccgccaggcc cgcgccgccg agctcgaggg cgaaatcgcc
     5221 tcgctggccg cccacatcaa cgccgccact taccgcttga tcgagatgct gcgggagttc
     5281 gacgagctcc ggggctggag cggctggccg acgccggcgc actggctgca gtatcgctgc
     5341 ggctactcgc tcggcgccgc gcgggagcgg ttccgtgtcg ccc
//
LOCUS       DAOMPG010000162         5379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6436_length_5379_cov_3.192900, whole genome shotgun sequence.
ACCESSION   DAOMPG010000162 DAOMPG010000000
VERSION     DAOMPG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6436_length_5379_cov_3.192900"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PLS34_09115"
     CDS             complement(<1..113)
                     /locus_tag="PLS34_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRP87666.1"
                     /translation="MLFMSATTKTPEEIEKMRVAGRLAAEVLQVVAEHVKPG"
     gene            complement(184..1134)
                     /locus_tag="PLS34_09120"
     CDS             complement(184..1134)
                     /locus_tag="PLS34_09120"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HRP87667.1"
                     /translation="MRSIMAMLLLLAGLLAAPGVMAQTCTATAPTVHFGTVPTPVPQS
                     DVTTPITINCSGGSSNGSLRICVGITPAMSPSGERRMFGPGVGSLAYDIYKNSGRTQR
                     WADNSATARELVQVSLNASGSGSTTMNMYARMSAGGSPANGAYHSGTTDDTISGSFHN
                     GNSCNSSTAPGTFNGSPFDVSVLVGGNCTITAAPLLDFGSVMGSTTTQIDSAVTLHAT
                     CNNQLPYTIALNAGQVVGNTIADRRLGLDGTGPGVLRYQLYRDAGRSQLWGDGTAGSV
                     HVGTGNGVQQDIIVYGRVPAGQAMPAAGTYRDTVTATITY"
     gene            complement(1134..3431)
                     /locus_tag="PLS34_09125"
     CDS             complement(1134..3431)
                     /locus_tag="PLS34_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HRP87668.1"
                     /translation="MNELDLPAIGRSQAPGVEVNPAEQRLYLEVWFGDRASGQLAEFR
                     LHDGQLYATPGTLRTLGILIDPALAGPDGLLPLSALPGLTADYQAANQRVVLQVPASL
                     RPTQRLGYRLPEPVQAQRDTGLLFNYDSYGRRQDGHDSLSVSGLVRVFGRLGALETSG
                     VQDLGDGERGFRRLDTRWTYSHAASMTTWTAGDLVNGGLAWTRPVRLGGLQWRRNFGV
                     RPDLISFPVPTFAGQATLPSSVELMVDNISQYRGEVGDGPFVIDSFPRISGAGDVTVV
                     VRDALGRAVETQVPLYIDYQRLAPGLSDFSMEAGYLRTGFATREDDYGNELAASASYR
                     RGLSDSFTIEGHAEYGDDLRLGGLGAVWSPSARYGVLNLSAARSSGLGTAGQHYSAGY
                     QWMNRRVGFDLQAQRFDTGFRDLGDLAEMPSTGVQRDRLRALDRASVWMAVPRGSVGY
                     SWVRTARAGNAGDERVQSLSWSQSWGRLFSYLSAYDSSRSGVGLSISVSLPLGETRSA
                     SLAVSRADDRTAVVAGLRQSPPYDGGLGWDLQAGHRDSDTFGQASATLRGDHGEATLG
                     VDRYGGRIGYFGQAAGSVVWMGGGVFASRRVNDSFAVVSTNGVADVPVLYENRELGLT
                     NRRGLFLVPDLRGWQRNHIAIDPDGLGAGYRLPPLELEATPADRGATLVEFDVARIHP
                     ALVALLDPAGAPIAAGTEGGLPGTDRTFMVGLDGVAWLEDHAAGDILELDTAAGTCRY
                     TLPASASGEEGAVVKLGPLACEGTR"
     assembly_gap    3468..3477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3487..3633
                     /locus_tag="PLS34_09130"
                     /pseudo
     CDS             3487..3633
                     /locus_tag="PLS34_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3667..>4066)
                     /locus_tag="PLS34_09135"
     CDS             complement(3667..>4066)
                     /locus_tag="PLS34_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HRP87669.1"
                     /translation="QGRDLSFRLVVDELPAPDSEQGSGIELRLRYLVPLFARARDAAE
                     PELACTLEATTLACRNTGGRPAQLGATRIVDDGGHSVALSEGLFGYLLPGTGRTWDVD
                     RSRVSGLRAGLKLEAQINGQPGTVPVQRQP"
     assembly_gap    4067..4107
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4108..4409)
                     /locus_tag="PLS34_09140"
     CDS             complement(<4108..4409)
                     /locus_tag="PLS34_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HRP87670.1"
                     /translation="MKRAIALAAVLALLPAGHAFARGQLQARPTLVELAPGNQAGRLV
                     LANTGDAPVAAQVRVFAWSQVDGEDRLADTNEIAISPAIVRIPPGGEQVVRVVRLG"
     gene            complement(4412..4903)
                     /locus_tag="PLS34_09145"
     CDS             complement(4412..4903)
                     /locus_tag="PLS34_09145"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HRP87671.1"
                     /translation="MRTQLATALALAVLTVAGHASAADTASFGVQLVVQNACNITAGG
                     TAADMDFGTTISNIPAAIDRTTNLSVTCSNGAAYRIGLGNGANESAGQRRMANGANFA
                     NYDLFRDDTRTAPWGALSTANALVGTGNGSLQSIPVYGRVPVQNGLVAGTYNDIVTAT
                     IEY"
     gene            4927..5160
                     /locus_tag="PLS34_09150"
     CDS             4927..5160
                     /locus_tag="PLS34_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87672.1"
                     /translation="MSGMAGSDADRGLLPATALTSHYANGVPTLLNHLMYLIFLIFSS
                     GCTLISKLFNDIFTARDALPATRLRNLTMRARM"
BASE COUNT          826 a         1815 c         1832 g          855 t
ORIGIN      
        1 ccgggcttca cgtgctcggc cacgacctgc agcacctcgg ccgccaggcg gccggccacg
       61 cgcatctttt cgatctcttc gggggttttg gtggtcgcgg acatgaacag cattatcccc
      121 gatccggcca tttcctcaag aaaagcgctg gccaggcgtg cccgaacccc ggcgacggcg
      181 tccctagtag gtgatggtcg cggtcaccgt gtcgcggtag gtgccggcgg ccggcatcgc
      241 ctgcccggcg ggtacacggc cgtagacgat gatgtcttgc tgcacgccgt tgccggtacc
      301 gacgtgcacc gagcccgcgg ttccgtcgcc ccacagctgc gagcgcccgg cgtcgcggta
      361 gagctggtaa cggagcacgc cggggccggt gccgtcgagc cccagccgac ggtcggcgat
      421 ggtgttgccc acgacctggc ccgcattgag cgcgatcgtg tagggcagct ggttgttgca
      481 ggtcgcgtgc agggtgacgg cgctgtcgat ctgcgtcgtc gtgctgccca tcacgctgcc
      541 gaagtcgagt agcggggcgg ccgtgatggt gcagttgccg ccgacgagca cactcacgtc
      601 gaacggactg ccgttgaacg tgccgggggc ggtactcgaa ttgcagctgt tgccgttgtg
      661 gaatgatccc gagatcgtgt cgtcggtggt cccggaatgg taggccccat tggcgggact
      721 gccgccggcg gacatccgcg cgtacatgtt catcgtcgta ctgccgctgc cgctggcatt
      781 caggctcact tgcaccagct cgcgcgcggt cgcgctattg tcggcccaac gctgggtacg
      841 accggaattc ttgtagatgt cgtaggccag ggaaccgaca ccaggcccga acatccggcg
      901 ttctccggag ggcgacatcg cgggcgtgat cccgacgcag attcgcaggc tcccattcga
      961 gctcccgccc gagcagttga tcgtgatcgg tgtcgttacg tcggattgcg gcaccggcgt
     1021 gggcacggtg ccgaaatgga cggtgggcgc ggtcgcggtg caggtctggg ccatgacgcc
     1081 tggcgcggcg agcaacccgg ccagcagcaa caacattgcc atgatcgaac gcatcagcgt
     1141 gttccctcgc aggccaacgg gccaagtttg accacagcgc cctcttcacc ggacgccgat
     1201 gcgggcaagg tgtagcggca ggtaccggcg gccgtgtcga gctccagtat gtcgccggcg
     1261 gcatggtcct caagccaagc cacgccgtcc aggccgacca tgaacgtccg gtccgtgccc
     1321 ggcaagccgc cctcggtgcc ggccgcgatc ggcgcgccag cggggtcgag cagggccacc
     1381 agcgccggat ggatgcgcgc gacgtcgaac tcgaccagcg ttgcgccacg atcggccggc
     1441 gtggcctcca gctccagcgg tggcaagcgg tagcccgcgc cgaggccgtc gggatcaatc
     1501 gcgatgtggt tgcgctgcca gccgcgcagg tcgggaacga ggaacaggcc gcggcgattg
     1561 gtcaggccca gttcgcggtt ctcgtacagc accggcacgt cggcgacgcc attggtgctg
     1621 accaccgcga aggagtcgtt gacgcggcgg ctggcgaaca cgccgccacc catccacacc
     1681 acgctgccgg cggcctggcc gaagtagccg atgcggccac cgtaacgatc gacgcccagc
     1741 gtcgcctcgc cgtgatcgcc gcgcagggtg gccgaggcct gcccgaacgt gtccgagtcg
     1801 cgatgcccgg cctgcaggtc ccagcccagg ccgccgtcgt agggcggcga ctgacgcagg
     1861 ccggcgacca cggcggtgcg atcgtcggcc cggctgaccg ccagggacgc gctgcgggtc
     1921 tcgcccagtg gcaacgacac gctgatgctc agcccgacgc cactgcggct gctgtcgtac
     1981 gcactcaggt aggaaaacaa gcgcccccac gactgcgacc agctcagcga ctgcacgcgc
     2041 tcgtcgccgg cgttgcccgc gcgcgcagtc cgcacccacg aatagccgac gctgccgcgc
     2101 ggcaccgcca tccagaccga ggcgcgatcg agcgcgcgca gccggtcccg ctggacaccg
     2161 gtcgatggca tctcggccag gtcgccaagg tcgcggaaac ccgtgtcgaa gcgctgcgcc
     2221 tgcaggtcga atcccacgcg tcggttcatc cactggtaac ccgcgctgta gtgctggcca
     2281 gcggtgccga ggccgctgct gcgtgcggcc gaaagattga gcacgccata tcgggctgag
     2341 ggcgaccaga ccgcgccaag cccacccagg cggaggtcgt cgccgtattc ggcatggccc
     2401 tcgatggtga agctgtcact gaggccgcgg cggtagctgg cactcgccgc aagctcgttg
     2461 ccatagtcgt cctcgcgcgt ggcgaagccg gtgcgcaagt agcctgcttc catgctgaaa
     2521 tcgctgaggc caggcgccag gcgctggtag tcgatgtaca gcggcacctg ggtttcgacc
     2581 gcacgcccga gcgcatcgcg caccaccacg gtgacatcac cggctccgga gatgcgcggg
     2641 aaactgtcaa tgacgaacgg tccgtcaccg acctcacccc ggtactggct gatgttgtcg
     2701 accatcagtt cgaccgacga cggcagcgtg gcctggccgg caaacgtggg gacaggaaag
     2761 ctgatcaggt ccgggcgcac gccgaagttg cgccgccact gcaggccgcc cagccgcacc
     2821 ggccgggtcc aggccagacc gccgttgacc aggtcgccgg cggtccaggt ggtcatgctc
     2881 gccgcgtggc tgtaggtcca gcgcgtatcg agcctgcgga agccgcgttc gccgtcgccg
     2941 agatcctgga caccgctggt ttcgagcgcg cccagacgcc cgaagacgcg cacaaggccg
     3001 gacacggaga ggctgtcgtg gccatcctgc cggcgaccat agctgtcgta gttgaacagc
     3061 aggccggtat cgcgctgggc ctgcacgggt tcgggcaggc gatagcccag gcgctgggtc
     3121 ggcctcagcg acgccggcac ctgcaggacc acgcgctggt tcgcggcctg gtagtcggcc
     3181 gtcaggcccg gcaacgccga aagcggcagc aggccatccg gcccggccag ggccggatcg
     3241 atgaggatgc caagggtccg cagggtgccc ggcgtggcat acagttggcc gtcatgcagg
     3301 cggaattccg ccagttggcc gctggcccgg tcgccgaacc agacttccag gtacaggcgc
     3361 tgctccgccg ggttgacctc caccccgggg gcctggctgc ggcctatcgc cggcaggtca
     3421 agttcattca tcgaataggt cgcggcactt gcaccgctcg tggcaggnnn nnnnnnngac
     3481 cggctgctgc cgccgctcga gccggccgag gcgctcgaag tcgcgaccat cgcgtcggtc
     3541 ggcggtcgcg gcgtcgatca tgcgagctgg ggctgccggc cgttccgcag cccgcatcac
     3601 actgcgtcgg ccagtgcgat cgacggcaac atcagcaatg cgagggcgat cgccccggcc
     3661 agccggtcag ggctggcgct ggaccggcac ggtacccggc tggccattga tctgtgcttc
     3721 cagcttcaac ccggccctga gcccggacac gcgactgcgg tcgacgtccc aggtccgacc
     3781 ggtcccaggc agcagatatc caaacaagcc ctcgctcagg gcgacgctgt gtccgccgtc
     3841 gtccacgatg cgggtcgcac ccagctgcgc cgggcggcca ccggtgttgc gacaggccag
     3901 cgtggtcgct tccagggtgc acgccagctc cggttcggcg gcgtcgcggg cgcgggcgaa
     3961 cagcggcacc aggtaacgca gccgcagttc gattccgctc ccctgttcgc tgtccggcgc
     4021 cgggagctca tccacgacca gccggaaact caggtcgcgc ccttgcnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg cccaggcgca cgacccggac cacctgttcg
     4141 ccacctggcg gaatcctgac gatcgccgga ctgatggcga tctcgttggt gtcggcaagg
     4201 cgatcctcgc cgtcgacctg cgaccaggcg aacacgcgca cctgcgccgc caccggcgcg
     4261 tcgccggtat tggccaggac cagccgcccg gcctggttgc cgggcgccag ttccaccagc
     4321 gtcgggcgtg cctgcagctg gccgcgcgcg aacgcgtggc cagccggaag cagcgcgagg
     4381 acggcggcaa gcgcgatcgc ccgcttcatg ctcagtactc gatcgtcgcg gtgacgatgt
     4441 cgttgtaggt cccggccacg aggccgttct ggaccggtac gcggccgtag acggggatcg
     4501 actggaggct gccgttgccg gtgccgacca gtgcgttggc ggtgctcagc gcgccccacg
     4561 gtgcggtgcg ggtgtcgtct cggaacaggt cgtagttggc gaagttggca ccgttggcca
     4621 tccggcgctg gccagcgctc tcgttggcgc cgttgccgag accaatcctg tacgccgccc
     4681 cgttgctgca ggtgacgctc aggttggtcg tgcggtcgat ggccgcggga atgttgctga
     4741 tggtggtgcc gaagtccatg tcggcagcgg ttccgccggc ggtgatgttg caggcgttct
     4801 ggacgaccag ctggacgccg aagctggccg tgtccgcggc actggcgtgg ccggcaacgg
     4861 tcaggacggc aagggcaagg gcggtggcga gttgggtacg cattgcaagt ttcctcagag
     4921 ggtgaaatgt ctggaatggc gggatcggat gccgatcgtg gactcctccc cgcgacagcc
     4981 ttgacctcgc attatgcaaa tggcgtgcca acactgttaa atcatctcat gtatttgatt
     5041 tttctaatat tttcatcagg atgcaccttg atttccaagc tttttaacga tattttcact
     5101 gctcgcgacg ctttgccggc gacccgcctc cggaacctga ccatgcgcgc ccgaatgtga
     5161 cgctggtccc gtcagtgcgg gctcggtcgc ggccggcgga caacctgttt caatccccga
     5221 tttccctcag gactctcgtc gggctagaat gcgcggcttg cttggcaccg tgcccggcaa
     5281 gccgcccacg ccgggcgaag cgctcttgat cgacgcaggc gaatacacac ccgctgcaac
     5341 cccccgttcc ggggtgtccc gagcctgcga aggcccggg
//
LOCUS       DAOMPG010000163         5344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6486_length_5344_cov_4.040083, whole genome shotgun sequence.
ACCESSION   DAOMPG010000163 DAOMPG010000000
VERSION     DAOMPG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_5344_cov_4.040083"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLS34_09155"
     CDS             complement(<1..300)
                     /locus_tag="PLS34_09155"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 family protein"
                     /protein_id="HRP87673.1"
                     /translation="MNTLAQPAPQWIPAAANAVGFNIVWAVTVFGAAAGLAWAGPAAL
                     LVFATLQLRLAARPHYDLAAMAVFASAGVVIDSAWSLAGALSYAAPWPAASLAPVW"
     gene            366..1061
                     /locus_tag="PLS34_09160"
     CDS             366..1061
                     /locus_tag="PLS34_09160"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRP87674.1"
                     /translation="MSDEKQHSDQFHSWRPPPWHGLEPGLNPPLEVRAYIEITPFDLV
                     KYEVDKASGYTIVDRPQRTSAAPPVLYGFIPQTYCDERVRRLTPGAKRADGDPLDICV
                     ISERQIERGDILLNSRVIGGIQMIDGGEADDKIIAVLRNDNIWSEANDIGDLPHVMIQ
                     RLQHYFSTYKMGPNMTSGVEITATYGRDHAAKVVTAAIRDYQDVFGASRRAWEKLEER
                     LMHPDESADPAAE"
     gene            complement(1084..1851)
                     /locus_tag="PLS34_09165"
     CDS             complement(1084..1851)
                     /locus_tag="PLS34_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRP87675.1"
                     /translation="MRRVIFNQKGGVGKSSITANLAAISAARGRDTLVVDLDPQGNSS
                     QYLLGDAWETAPGDVAAFFNDSLAIRILPRDPLRYVTPTDYEGLHVLASSPELAELQV
                     KLEARHKIYKLRELLDKLAEQFDAIYIDTPPAFNFYTLSALIAADACLIPFDCDDFSR
                     RALYTLLENVEETRADHNPGLRVEGIVINQFLSRANLPQRLVDELREEGLPVLETKLS
                     SSVKMRESHEQCVPLVHLAPRHKLTEEFLALYDELGG"
     gene            complement(1863..2417)
                     /locus_tag="PLS34_09170"
     CDS             complement(1863..2417)
                     /locus_tag="PLS34_09170"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRP87676.1"
                     /translation="MSLRISFELSARDVKHFREAMRKARRTVRDADDAEILDAARMLF
                     TDVSATRVPGYVRERIGRLRAMVDMVEDKEWRLSAAEQERVLAALVYFCDPEDLIPDD
                     IPGLGFLDDAIMVELAFRELKHEIEAYQDFCEYRRKYDSGFRLRRSPEARRQKLDARE
                     AQLRERAARRRERDQENGATQKLL"
     gene            2566..3501
                     /locus_tag="PLS34_09175"
     CDS             2566..3501
                     /locus_tag="PLS34_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRP87677.1"
                     /translation="MTMFRSIAVALALLGPGGTLAATFILPPEGTDLVGVPSWVHTRY
                     ADTLMDVARDYSVGYTELKRANPGVDTWIPGDGTRVLLPTRYVLPPGPREGIVINLPE
                     YRLYYFPAPKKGERAQVVTYPISIGKMDWNTPIGRHRIISKAKNPSWYPPDSIREDHA
                     KRGSILPRVVPPGPDNPLGAYAMRLNLTSYLIHGTNNPDGVGMRVTSGCIRMFPEHIE
                     RIFPMVDVGTTVHIISEPIKVGWAADTLFVEVHPPLEEESESQLRMRFTALLREAAQL
                     RGVAIDWPRADLIFAAASGVPTEVPVREVTVASQP"
     gene            complement(3621..3920)
                     /locus_tag="PLS34_09180"
     CDS             complement(3621..3920)
                     /locus_tag="PLS34_09180"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HRP87678.1"
                     /translation="MKKNFLYAGVAVIALGVMGGCATTELDAVRATAEQALNEARAAK
                     TTADAARAAADEAKSVAANAQSTASRAAQAASEAQACCAANTDRIERMFQRGMSK"
     gene            4177..4800
                     /gene="lexA"
                     /locus_tag="PLS34_09185"
     CDS             4177..4800
                     /gene="lexA"
                     /locus_tag="PLS34_09185"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012442797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRP87679.1"
                     /translation="MRDRLTARQRQVLDLVRENIEESGMPPTRAEIATALGFRSANAA
                     EEHLRALARKGAIELLPGASRGIRLKGASREPEGLPLVGRVAAGRPMLAEEHIQGRYQ
                     IDPALFRPRPHYLLQVHGMSMKGAGILDGDLVAVHRTPEVRSRQIVVARLDDEVTVKR
                     YRQEGKIVWLLPENADFEPIRVDLAEQAIIIEGVVVGVVRKDLAQRS"
     gene            4810..>5344
                     /gene="imuA"
                     /locus_tag="PLS34_09190"
     CDS             4810..>5344
                     /gene="imuA"
                     /locus_tag="PLS34_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HRP87680.1"
                     /translation="MSESLHTLLEDPRLWRAGRRGRHWPTLPSGHPPLDALLPGGGWP
                     ADRLTEIAVERWGSGELALLMPLLARLSRADAAGWVAWIAPPFLPYAPALAAAGVDLA
                     RLLLVRARDDAEALWATEQTLHSGACRLVLAWAGRADGRCLRRLQLAAESSRTALILF
                     RPPTTLAVPSPAALRLSL"
BASE COUNT          848 a         1801 c         1849 g          846 t
ORIGIN      
        1 ccagacgggc gccagcgacg cggcgggcca gggcgcggca tagctcaggg cgccggccag
       61 cgaccaggcg ctgtcgatca cgaccccggc gctcgcgaac accgccatcg cggccaggtc
      121 gtagtgcgga cgggcggcca ggcgcagctg gagtgtggcg aacaccagta gcgcggcggg
      181 gccggcccag gccagcccgg cggccgcccc gaataccgtg acggcccata cgatgttgaa
      241 gccgacggcg ttggccgcgg cgggaatcca ctggggggca ggctgggcga gtgtgttcat
      301 gtaagctagt tcgccaggcg ccgcggggcg gattcggggc gcgtcaccag ggaggattcc
      361 ggccgatgag cgacgagaag caacattccg accagttcca cagctggcgg ccccccccct
      421 ggcacgggct ggagcccggc ctcaacccgc ccctggaagt cagggcctac atcgagatca
      481 cgccattcga cctggtcaag tacgaggtgg acaaggcctc cggctacacc atcgtcgacc
      541 gcccgcagcg cacctccgcg gcgccgccgg tgttgtacgg gttcatcccg cagacctact
      601 gcgacgagcg ggtgcggcgg ctcacgccgg gggcgaagcg cgccgacggc gacccgctcg
      661 acatctgcgt catcagcgag cgccagatcg agcgcggcga catcctgctg aactcgcgcg
      721 tcatcggcgg catccagatg atcgacggcg gcgaggccga cgacaagatc atcgccgtgc
      781 tgcgcaacga caacatctgg agcgaggcga acgacatcgg cgacctcccg cacgtcatga
      841 tccagcgcct gcagcactac ttcagcacct acaagatggg tcccaacatg acgtcgggcg
      901 tggagatcac ggcgacgtac gggcgcgacc atgccgccaa ggtcgtcacg gcggcgattc
      961 gcgactacca ggacgtgttc ggcgccagcc gccgggcctg ggagaagctc gaggagcgcc
     1021 tgatgcatcc cgacgagtcc gcggaccccg cggcggagta gctcgcccgg gcggcgcaca
     1081 cggtcagccg cccagctcgt cgtacagcgc gaggaattcc tcggtcagct tgtggcgggg
     1141 cgcgaggtgg accagcggca cgcattgttc gtgcgactcg cgcattttca cgctcgagga
     1201 caacttggtc tcgagcaccg gcaggccttc ttcgcgcagt tcgtccacca gccgctgcgg
     1261 caggttggcg cgcgacagga actggttgat cacgatgcct tcgacgcgca gcccgggatt
     1321 gtggtcggcg cgggtctcct cgacgttctc gagcagcgta tagagcgcgc gccgcgagaa
     1381 gtcgtcgcag tcgaagggga tcaggcaggc gtccgcggcg atgagcgccg acagggtgta
     1441 gaagttgaag gcgggcggcg tgtcgatgta gatcgcgtcg aactgctccg ccagcttgtc
     1501 gagcagctcg cggagcttgt agatcttgtg gcgggcttcc agcttgacct gcagctcggc
     1561 gagttccggc gaggaggcga gcacgtgcag cccctcgtag tcggtcgggg tcacgtagcg
     1621 cagcgggtcg cgcggcagta tgcggatggc cagcgagtcg ttgaaaaatg ccgccacgtc
     1681 gccgggtgcc gtttcccacg catcgccgag caggtactgg ctggaattgc cctggggatc
     1741 gagatcgacc accagcgtat cgcggccgcg ggccgcgctg atcgccgcga gattcgccgt
     1801 gatgctcgac ttgccgacgc cacccttctg gttgaagatc actcgccgca taagggcgtg
     1861 gcctacagca gcttctgcgt ggcgccgttt tcctggtcgc gctcacgacg acgcgcggcg
     1921 cgctcgcgaa gttgcgcctc gcgcgcatcg agcttctgcc tgcgcgcctc cgggctgcgc
     1981 cgcaggcgga agccggagtc gtacttgcgc cggtattcgc agaaatcctg gtaggcctcg
     2041 atctcgtgct tcagttcgcg gaaggccagc tcgaccatga tggcgtcatc gagaaaaccg
     2101 agccccggga tgtcgtcggg aatcaggtcc tcgggatcgc agaaatacac cagcgccgcc
     2161 agcacgcgtt cctgttcggc ggcgctcaag cgccattcct tgtcctccac catgtcgacc
     2221 atggcgcgca ggcggccgat gcgctcccgc acgtagcccg gcacccgcgt ggcactgacg
     2281 tcggtgaaca gcatcctggc cgcatcgagg atctcggcgt cgtccgcgtc gcgtaccgtg
     2341 cggcgcgcct tgcgcatcgc ctcgcggaaa tgcttcacat ccctggcgct gagttcgaaa
     2401 gatattctta ggctcatggg caagctccgc gggcggaagc aggaagccta agggccgcct
     2461 gtgggagcgt caaccagccg cgccgcggcc cggcgtggct tcggcccgtt tggcaggtaa
     2521 aatgggcacc aggtgcaagg tgcccagggc cggaaaggcg aattgatgac aatgtttcga
     2581 tccatcgcgg tggcgctggc gctgctgggt cccggcggga ccctggcggc gaccttcatc
     2641 ctgccgccgg aaggtaccga tctcgtcggg gtgcccagct gggtgcacac gcgctatgcg
     2701 gacacgctga tggacgtggc gcgcgattac agcgtcggct acacggagct gaagcgggcc
     2761 aatccgggcg tcgacacctg gatccccggc gacggcacgc gcgtgctgct gccgacccgc
     2821 tacgtgctgc cgccggggcc gcgcgagggc atcgtcatca acctgcccga ataccggctg
     2881 tactacttcc ccgcgccgaa gaagggcgag cgcgcccagg tcgtcaccta ccccatcagc
     2941 atcggcaaga tggactggaa cacgcccatc ggtcgccatc gcatcatcag caaggccaag
     3001 aacccttcct ggtatccgcc ggactccatt cgcgaggacc atgccaagcg cggcagcatc
     3061 ctgccgcgcg tggtgccgcc cggaccggac aatccgctcg gcgcttacgc catgcggctc
     3121 aacctgactt cgtacctgat ccacggcacc aacaatcccg acggcgtcgg catgcgcgtg
     3181 acctccggct gcatccgcat gtttcccgag cacatcgagc ggattttccc gatggtggac
     3241 gtcggcacca ccgtgcacat catcagcgag ccgatcaagg tgggctgggc ggcggacacg
     3301 ctgttcgtcg aggtccatcc gccgctcgag gaggagagcg agtcgcagct gcggatgcgc
     3361 ttcaccgcgc tgctgcggga ggccgcgcag ctccgcggcg tggcgatcga ctggccgcgc
     3421 gcggacctga ttttcgccgc tgccagcggc gtgccgacgg aggtgccggt gcgcgaggtg
     3481 acggtcgcct cacaaccgtg aatgggaatt gggcacattc cccaatcacc tgaacgtcat
     3541 tggggaacgt ccccaattcc cccgtcgcaa aagaaaaggc cgcagcaagc tgcggccttc
     3601 tcgtttgggc gagcgtgtcc ttacttggac atgccgcgct ggaacatccg ctcgatgcga
     3661 tccgtgttgg cggcgcagca ggcctgggcc tcgctggcgg cctgggcggc gcggctggca
     3721 gtgctctggg cattggcagc aacgctcttc gcttcgtcgg cggccgcacg ggcggcgtcg
     3781 gcggtggtct tggcggcgcg ggcctcgttc agggcctgct cggccgtggc gcggacagca
     3841 tccagttcgg tcgtggcgca gccacccatc acgcccagcg cgatgacggc gacgccggca
     3901 tacaggaagt tcttcttcat ggatttaaat cccctggtga tcacgatgat ttttcgcggt
     3961 ttttcgcatg cggaaacacg gctgattcat tctgccgtta cataatatgc ttcggtagtt
     4021 tacgaaatcc ggctttgtaa atcaaccagc taggtcgctg gaagcgggcc gggcgccgcc
     4081 ttcggcgagc cggaaggacc ccggaacagg gacttgccgc cggcgcctgg ctgtatataa
     4141 tcgcaagtac tgttcatcct tacagaggcg ccggacatgc gagacaggct caccgcgcga
     4201 cagcgccagg tcctcgacct ggtgcgcgag aacatcgagg aatccggcat gcccccgacc
     4261 cgggcggaga tcgccacggc gctgggcttc cgttccgcga acgccgccga ggaacacctg
     4321 cgggcgctgg cgcgcaaggg cgccatcgaa ctgctgcccg gcgcgtcgcg cggcatccgt
     4381 ctgaaagggg cgtcgcgcga gccggagggc ctgccgctgg tcggacgggt tgcggcgggc
     4441 cgaccgatgc tcgccgagga gcacatccag gggcgctacc agatcgatcc ggcgctgttc
     4501 cggccgcgcc cgcactacct gctccaggtc cacggcatga gcatgaaggg cgccggcatc
     4561 ctcgacggcg acctggtcgc ggtccaccgc actccggagg tgcgcagccg gcagatcgtc
     4621 gtcgcccggc tggacgacga agtgaccgtg aagcgctacc ggcaggaagg caagatcgtg
     4681 tggctgctgc cggagaacgc cgatttcgag ccgatccgcg tggacctggc cgagcaggcc
     4741 atcatcatcg agggcgtcgt cgtgggggtg gtgcgcaagg acctcgccca gcgcagctga
     4801 cgcgtcgtca tgagtgagtc gctgcacacc ctgctggagg acccgcggct ctggcgggcc
     4861 gggcgtcggg gtcgccactg gccgaccctg ccgagtggcc acccgccgct tgatgcgctg
     4921 ctgcccggcg gcggctggcc tgcggaccgg ctcaccgaga tcgcggtcga gcgctggggc
     4981 agcggcgaac tcgcgctgtt gatgccgctg ctggcgcgcc tctcccgtgc cgacgcggcc
     5041 ggctgggtcg cctggattgc cccgccgttc ctgccctatg cgccggccct ggcggcggcc
     5101 ggtgtcgacc tggcgcggct gctgctggtg cgcgcacgag acgacgccga ggcgctctgg
     5161 gccactgagc agacgctgca ttccggtgct tgccggctcg tgctggcctg ggccggacgg
     5221 gccgacgggc gctgcctgcg gcgcctgcag ctggccgccg agagcagtcg caccgcgctg
     5281 atcctgtttc gcccgccgac gacgctcgcc gtgccgtcac cggccgcctt gcgcctcagc
     5341 ctgg
//
LOCUS       DAOMPG010000164         5333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6499_length_5333_cov_4.118037, whole genome shotgun sequence.
ACCESSION   DAOMPG010000164 DAOMPG010000000
VERSION     DAOMPG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_5333_cov_4.118037"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..763)
                     /locus_tag="PLS34_09195"
     CDS             complement(146..763)
                     /locus_tag="PLS34_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRP87681.1"
                     /translation="MPDTKLARTSLPVHELIARRWSARAIDPGRRVARDHLVVLLEAA
                     RWAPSCSGDEPWRYIAWDRFDNPAGWQLAWDCLKPGNQYWVKHAPVILASFADSEFRS
                     GKPNRWGQHDTGAASENLCLQGAALGLVVHQMGGFDKAKLAAACAVPERFIPMAMIAV
                     GHPGDPTVLSEKHQAAETAPRQRRPFGELFFDGAWEQPLADGARG"
     gene            865..1545
                     /locus_tag="PLS34_09200"
     CDS             865..1545
                     /locus_tag="PLS34_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRP87682.1"
                     /translation="MHNPVNGKASDLRSAGKRLQSAGEELANSVSHGAGFVSAAVGTP
                     FLLAEVAGNGGVADLVGAAVFVVTVLLAYLASTLYHAVPWPRAKGVLRAIDHSSIFLL
                     IAGTYTPFTLGVLRGGWGWTLFGMVWTLALAGVIMKSLGGVRFHKLSMAVYLGMGWLI
                     VIAIVPLVERMAPGGLAWLVAGGLAYTGGVLFYALDRYRYCHFIWHLFVLAGTACHFV
                     AVMRYAAG"
     gene            1685..2587
                     /locus_tag="PLS34_09205"
     CDS             1685..2587
                     /locus_tag="PLS34_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HRP87683.1"
                     /translation="MPELDSAREGLAAFRGFRTRFRVVGRGRARRPLLCIHGGPGLGM
                     AYLQPLEEMHTTGRQVVFYDQLGCGGSDHPGTDFDWSLELFLDELDAVRAAAGLDTCH
                     LLGHGWGGMLALEHALRRPAGIASLVLSSAVASVPQWRRELVTLVAALPEEIRLPLYQ
                     HSAAGTAGSPDCCCVVDAVLRRNLCRLSPWPDCLERSHREARQHPEARNAMLGPNELE
                     PAGQLAGWDVTSRLGEIKSPTLVVSGRHDLVTPPMAAALYQGIPGSEWVVFEHSAHVP
                     HLEEPQRYLEVLDGFLDKAEDAQR"
     gene            complement(2607..3104)
                     /locus_tag="PLS34_09210"
     CDS             complement(2607..3104)
                     /locus_tag="PLS34_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRP87684.1"
                     /translation="MLRLILLRHGKSDWDDPSLDDFHRPLAPRGLRDVPEMGRRLARR
                     GQVPDLIISSSAVRAISTARAVARELGYREDGIVEDAGLYHAPPGSMLAIIGRAPATA
                     GTLMVVGHNPGMTELANMLDDVRLDNMPTAGMLCVEFAAADWGAIEPAAARFAWFDYP
                     KKRSD"
     gene            complement(3201..3467)
                     /locus_tag="PLS34_09215"
     CDS             complement(3201..3467)
                     /locus_tag="PLS34_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRP87685.1"
                     /translation="MASWHDVTAADDIEPGGFALLETDELTAAVFNVDGTLYAIEDVC
                     THDGAELAGGPVVGDQVVCPRHGARFCLRTGAALCPPAYEPVGR"
     gene            complement(3467..4018)
                     /gene="sufT"
                     /locus_tag="PLS34_09220"
     CDS             complement(3467..4018)
                     /gene="sufT"
                     /locus_tag="PLS34_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HRP87686.1"
                     /translation="MYGQDSEPVTLARDCTAIIVPAGDQVILRKGARGLITQALGGSF
                     TVYLDGNLFRIAGEDADALGKEPVLPPQLAPDAADEDVEDLVWEQLRTCFDPEIPINI
                     VDLGLIYHCKLLPAQGGDRDVEVTMTLTAPGCGMGDVLVMDVKDKLEIIPTIREARVE
                     LTFDPPWNRDMMSEAARLQTGMF"
     gene            complement(4028..4471)
                     /locus_tag="PLS34_09225"
     CDS             complement(4028..4471)
                     /locus_tag="PLS34_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019218124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRP87687.1"
                     /translation="MNLADLYRDVIIDHNRKPRNFRKIPPPCSHAEGLNPLCGDKLQV
                     YLKLGADDRIEDVAFEGSGCAISVASASLMTEALRGKTRAEAEDMFARIHGMLTGAEE
                     PQVDSIGKLAALAGVREYPSRVKCASLCWHTMHSALGQGGTVSTE"
     gene            complement(4471..>5333)
                     /locus_tag="PLS34_09230"
     CDS             complement(4471..>5333)
                     /locus_tag="PLS34_09230"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRP87688.1"
                     /translation="MEHHANIVPWQMLCEQTGAVLKVIPITMAGELDLDAARGLVGPR
                     TRLLACGHVSNALGTINPVEELVALARSVGALVLLDGAQGVPHMRVDVRALGCDFYAF
                     SAHKMFGPTGIGVLWGRQELLAAMPPWQGGGDMILAVSFDGTTYNELPWKFEAGTPHI
                     AGAIGLGAAIDYLERLDFERAAAWEQELLEYGTAALGEIDGLRIIGTAPHKAAVISFV
                     LDGVHPHDIGTIVDHAGVAIRTGHHCAMPVMEFYGVPATARASFAFYNTKAEIDRLAA
                     ALRHAREVFA"
BASE COUNT          772 a         1792 c         1877 g          892 t
ORIGIN      
        1 gtggggggtg tcataggccg gccgagcgag tttttcctcg tccgagcgca ggccggcgta
       61 tggcgccgcg cgcccgcagt cgacccggaa cgaagccccc cgcccgcggt cgaccggaaa
      121 ctggtgtctg accccggttt cgggcctagc cccgcgcgcc atccgccagc ggctgctccc
      181 aggcgccgtc gaagaacagt tccccgaacg gtcggcgctg acgcggcgca gtctcggccg
      241 cctggtgctt ttccgacagc accgtcgggt cgccgggatg gccgaccgca atcatggcca
      301 tcggtatgaa gcgctccggc accgcgcagg ccgcggccag cttcgccttg tcgaaaccac
      361 ccatctggtg gaccaccagc cccagcgccg caccctgcag gcaaaggttc tccgaggccg
      421 cgccggtgtc atgctggccc cagcggttcg gcttacctga gcggaattcc gaatccgcga
      481 aggaggccag gatcacgggc gcgtgcttga cccagtactg gttgcccggt ttcaggcagt
      541 cccacgcgag ctgccagccg gcggggttgt cgaagcggtc ccaggcgatg tatcgccacg
      601 gctcgtcgcc gctgcacgac ggcgcccagc gggcggcctc cagcagcacg acgaggtgat
      661 cgcgcgcgac gcgccggccc gggtcgatgg cgcgggcgct ccagcgccgc gcgatgagtt
      721 cgtggacggg cagggaggtg cgggcaagct tggtgtccgg catgggcaag tccttttggc
      781 gggatgtagc tcaccccggt gacggttggc gtcattctac ccggcccgtt tgacgctccg
      841 cgtctcgaac gatacattga cgcaatgcac aatcccgtca acggcaaggc cagcgatctc
      901 cgcagcgcgg gcaagcgcct gcagtcggcc ggcgaggagc tcgcgaacag cgtcagccac
      961 ggcgccggat tcgtgtccgc ggcggtcggc acgccgttcc tgctggccga ggtggcgggc
     1021 aacggcggcg tggccgacct ggtcggcgcc gcggtgtttg tcgtcaccgt gctgctggcc
     1081 tacctggcct cgacccttta tcacgccgtg ccgtggccgc gtgccaaggg tgtcctgcgg
     1141 gcgatcgacc acagctccat tttcctgctg atcgcgggca cctacacgcc gtttacgctc
     1201 ggcgtgctgc gaggcggctg gggctggacg ctgttcggca tggtctggac gctggcgctg
     1261 gcgggcgtga tcatgaaatc gctcggcggc gtgcggttcc acaagctgtc gatggccgtc
     1321 tatctcggca tgggctggct gatcgtgatc gccatcgtac cgctggtcga gcgcatggct
     1381 ccggggggcc tcgcctggct ggtggcgggc gggctggcct acaccggcgg tgtactcttc
     1441 tatgcgctgg accgataccg ctactgccac ttcatctggc acttgttcgt gctggccggt
     1501 acggcctgcc atttcgtcgc ggtcatgcgc tacgccgcgg gctgacgcca cccgccggcc
     1561 gcggcgggcc gccgcggtcc ctgcttcggc attccctgtc ggtatctctc ctgatggcgg
     1621 tcaggcggcc gcatcctaag ctgagtccag tgcacaccca tggacaggac ggggtgcggg
     1681 ccgcatgccg gagctagatt cagccaggga aggcttggcg gcctttcgtg gttttcgtac
     1741 gaggttccgg gtcgtcggcc ggggccgcgc gcgtcgtccg ctgctctgca tccatggcgg
     1801 ccccggcctg ggcatggcct atctccagcc gctcgaggaa atgcacacta ccgggcgaca
     1861 ggttgttttc tacgaccagc tcggctgcgg gggctcggat catcccggca cggatttcga
     1921 ctggtcgctc gagctgtttc tcgatgagct ggatgcggtg cgcgccgccg ccggcctcga
     1981 tacgtgccat ctgcttgggc acggctgggg cggcatgctc gcgctggagc acgcgctgcg
     2041 ccggcccgcc gggatcgcca gcctggtgct ttccagcgcg gtggcgagcg taccgcagtg
     2101 gcgacgtgaa ctcgtgaccc tggtcgcggc cctgcccgag gagatccgcc tgccgcttta
     2161 ccagcacagt gccgccggca cagccggttc gccggattgc tgctgcgtcg tcgacgccgt
     2221 cttgcgccgc aacctgtgcc ggctctctcc ctggccggac tgcctcgagc gcagccaccg
     2281 ggaggcccgc cagcatccgg aagcccgcaa cgccatgctc gggccgaacg agctggagcc
     2341 ggccggccag ctggcgggat gggacgtcac gtcgcgcttg ggcgagatca agagtccgac
     2401 gctggtggtc tcggggcggc acgacctcgt cacgccgccg atggcggcgg cgctctacca
     2461 gggcataccc ggctccgagt gggtggtgtt cgagcacagc gcccacgtgc cgcatctcga
     2521 ggagccgcag cgctacctcg aagtgctgga cggcttcctc gacaaggccg aggacgcgca
     2581 gcgctagcgc gtccgggcaa cgcgattcag tcggagcgtt tcttcgggta gtcgaaccag
     2641 gcaaagcgtg ccgcggcagg ctcgatggcg ccccagtcag cggcggcgaa ctcgacgcac
     2701 agcatgccgg cggtcggcat gttgtcgagg cgcacgtcgt ccagcatgtt ggcgagctcg
     2761 gtcatgcccg ggttgtggcc gaccaccatc agcgtgcccg ccgtggccgg cgcacggccg
     2821 atgatcgcca gcatggatcc gggcggcgca tggtagaggc cggcgtcctc aacgatgccg
     2881 tcttcgcggt aaccgagctc ccgcgccact gcgcgcgcgg tgctgatggc gcggacggcg
     2941 ctgctggaga tgatcaggtc gggaacctgg ccgcggcgcg ccaggcgtcg gcccatttcc
     3001 ggcacgtcgc gcaatccgcg tggtgccagc gggcgatgga aatcatccag gctgggatcg
     3061 tcccagtccg attttccgtg tcgcaggagg atcaggcgca gcatcgacat cgcccggcac
     3121 tcagtccggc agctcgatct cgacccgtcc gtcgttgatg cgcacgggaa aggtctgcac
     3181 ggccgcgtag gcgggcggcg tcagcgcccg accggttcgt aggccggtgg acacagagcg
     3241 gcaccggtgc gcaggcagaa gcgcgcgccg tgacgcgggc acacgacctg gtcgccgacc
     3301 acgggcccgc cagcaagctc ggcaccatca tgggtgcaca cgtcctcgat ggcgtacagc
     3361 gtgccgtcga cgttgaacac ggcggccgtc agctcgtcgg tttcgagcag cgcgaagccg
     3421 cccggttcga tgtcgtccgc cgccgtcacg tcatgccaac tggccatcag aacatccccg
     3481 tctgcaatcg ggccgcttcc gacatcatgt cgcgattcca cggcgggtcg aaggtcagct
     3541 cgacacgcgc ctcgcgaatg gtcgggatga tctcgagctt gtccttcacg tccatgacca
     3601 gcacgtcgcc catgccgcag cccggcgcgg tcaacgtcat ggtgacttcc acgtcacggt
     3661 cgccgccctg ggccggcagc agcttgcaat ggtaaatgag gccgagatcg acgatgttga
     3721 tgggaatctc cggatcaaag caggtgcgca gctgctccca gacgaggtcc tcgacgtctt
     3781 cgtcggcggc gtcgggcgcc agctgcggcg gcaggaccgg ttccttgccg agtgcgtcgg
     3841 cgtcctcgcc ggcgatgcgg aacaggttgc cgtccaggta gacggtgaag ctgccgccca
     3901 gcgcctgcgt gatgagtccg cgggcgccct tgcggagaat gacctggtcg ccggccggca
     3961 cgatgatggc cgtgcagtcg cgcgccaggg tgacgggttc gctgtcttgt ccgtacatga
     4021 ctgtctgcta ctcggtactg actgtgccgc cctggccgag ggcggaatgc atggtgtgcc
     4081 agcagaggct ggcgcacttg acgcggctcg gatactcgcg cacgccggcc agcgcggcaa
     4141 gcttgccgat ggaatcgacc tgtggttctt cggcgccggt gagcatgccg tggatgcgcg
     4201 cgaacatgtc ctcggcctcc gcgcgggtct tgccgcgcaa cgcctcggtc atcagcgacg
     4261 ccgaggccac cgagatggcg cagccggagc cttcgaaggc gacgtcctcg atgcggtcgt
     4321 cggcaccgag cttcaggtag acctgcagct tgtcaccgca gagggggttg aggccctcgg
     4381 catggctgca gggtggcgga atcttgcgga aattccgcgg cttgcggttg tgatcgatga
     4441 tcacgtcccg atagagatcg gcgaggttca tcaggcgaac acctcgcggg catggcgcaa
     4501 cgccgctgcc agtcggtcga tctcggcctt ggtattgtag aaggcgaacg acgcgcgggc
     4561 cgttgcgggt acgccgtaga actccatgac cggcatggcg cagtgatgcc cggtgcggat
     4621 cgcgacaccg gcatggtcca cgatggtgcc gatatcgtgc gggtggacgc cgtcgagcac
     4681 gaacgagatc acggccgcct tgtgcggcgc cgtgccaatg atccggaggc cgtcgatttc
     4741 gcccagcgcc gcggtgccgt attccagcag ttcctgctcc caggccgccg cgcgctcgaa
     4801 gtcgaggcgc tcgaggtagt cgatcgcggc gccgagcccg atggccccgg caatgtgtgg
     4861 tgtaccggcc tcgaacttcc acggcagttc gttgtacgtg gtcccatcga agctgaccgc
     4921 gagaatcatg tcgccgccgc cctgccaggg cggcatggca gcgagcagtt cctggcggcc
     4981 ccagagcacg ccgatcccgg tggggccgaa catcttgtgg gccgagaagg cgtagaagtc
     5041 gcaacctagg gcgcgcacgt cgacccgcat gtgcggcacg ccctgggcgc cgtcgagcag
     5101 caccagcgca ccgaccgacc gggccagcgc caccagctcc tcgaccgggt tgatggtgcc
     5161 gagcgcgttg gagacgtggc cgcaggcgag caggcgggta cgcggtccca ccaggccgcg
     5221 ggcggcgtcc aggtcgagct cgccggccat ggtgatgggg atgaccttca ggaccgcccc
     5281 ggtctgctcg cagagcatct gccacggcac gatgttggcg tggtgttcca tgc
//
LOCUS       DAOMPG010000165         5324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6513_length_5324_cov_5.194914, whole genome shotgun sequence.
ACCESSION   DAOMPG010000165 DAOMPG010000000
VERSION     DAOMPG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6513_length_5324_cov_5.194914"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..4359)
                     /locus_tag="PLS34_09235"
     CDS             complement(379..4359)
                     /locus_tag="PLS34_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRP87689.1"
                     /translation="MLETWVPPAVALAYAVLLFLVAYVGDRLHRRGVYVPTPVVYSLA
                     LGVYCTSWTFFGAVGMSAAKGWDFLTIYLGPIIVFLMMTRLPARIVAVSKRQSITSIA
                     DFIASRYGKSQGIAALASVMALVTVLPYIALQLKAVAGSYSLITGGAGMDTVPRISAD
                     TGLLAAGLLAVFTILFGTRQVDSTESHRGMVLAVAFESAVKLVAFLAVGLWVLYGLFD
                     GPGALLEASRANETVTALYASATIGHSFLVLTVISMLAILCLPRQFHVAVVENTSPRD
                     LALARWTFPLYLALFTAFVVPITAAGIITQGTGADADGFLISLPMVAGREGLALLAFL
                     GGFSAATGMVIVATIACSTMLCNEIVMPALVKLRGEEVSSRPDLARLLIRIRRLLIVG
                     ILALAWLAYRVIGMYGALASIGLLSFALAAQFGPAMIGGLYWRRATRSGAIAGMTVGF
                     AVWIWTLLVPAFAQTGWLPAELVTAGPRGIAWLSPYALFGLDGLDPVTHGVVWSLGAN
                     IACFVGVSWFTVPRLMERRQAAAFVDGPGGPGGHGGPLPFLERFKGSAKVGDMRILAE
                     RFIGREKAVAAFEAHLHARGLPLSDGLRADPQVLDLTERLLSGAMGASAARTVLLSAL
                     KGAELQIEDVASIVGEASQISRFNRDLLETTLENVAEGISVIDADNRLVVWNRRYAEL
                     FKYPDHLLRIGTPVEALIRYNAELGRCGPGDVEEMVARRMAHFRRGEPHVFQRTRDDG
                     SVIEMRVNPLPGGGYVTSFSDITEHKRTELALRESERNIRIYTDNVPVLIAYLDRDMV
                     YRFVNKAYEQAIGLDRDEICGRRVDQIIGRESFLEREAHMEGALAGMRQSFEVATLDE
                     DGRQRYAEATYIPQFAADGKVQGFFALFHDITERRRAQQGLKEAYDTLEQRVQDRTRE
                     LSEVNRRLRSEIELRNRIERALRDAKADAEAANLSKTRFLAAASHDLLQPLNAARLFT
                     SALGQGDHDARTLRAVDRIDSSLRAAEELLTALLDISKLDAGALEPRHTDFRVDDILA
                     TLGVEFGIIARERGIEFRTVPCRVTVHSDRQFLRRILQNFLSNALRYTRQGKVLLGCR
                     RRGDTLVIEVLDTGPGIPAEHLEKDIYEEFRRFQPRDELGAKGMGLGLAIVQRMARAL
                     GHEVRVRSELGRGSVFSVEVPIARSQAAVASLPAMPRPAARLDEARVLCVDNEHSILE
                     GMARLLEGWSCAVLTATDTPGALAVLEDAACPPDLVLADFHLDGGDTGLKTIEAVRAR
                     WGDVPAVIITADYGEQAREHVRNAGFAIMRKPVKPAALRAIMSQLLLARRQGVIDAAR
                     ALADSA"
     gene            4604..4870
                     /locus_tag="PLS34_09240"
     CDS             4604..4870
                     /locus_tag="PLS34_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRP87690.1"
                     /translation="MSQLSAENRAEHWRRTRTLMIIHLVVWFIFSYVVHWFAVPLNNI
                     SFLGWPLGYYMAAQGSLIVFVVQLFLFNRQQHAIDVQFGVAEDE"
     gene            4883..>5324
                     /locus_tag="PLS34_09245"
     CDS             4883..>5324
                     /locus_tag="PLS34_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRP87691.1"
                     /translation="MGMQWEGKSFIDNLPKIYGVYTGGFLAFFALMAVLEAYAGLGAD
                     TIGILFIAFTIGIYAFIGILSRTMAVEAYYVAGRSVPPVFNGMATAADWMSGASFVAM
                     AGGVFMAGHGYMAFIVGWTGGYILVATLMAPYLRKFGCYTVPDFI"
BASE COUNT          861 a         1843 c         1815 g          805 t
ORIGIN      
        1 catgggcgcg cttcgttcgg cgaagtctga gctcactcag cgcgcccata acaccccggt
       61 cccgctgccg tgccgaacac ggcaacgcct tgccttcgca gcgccgctga cgatcgccac
      121 gctggcgatc ggctttgcgg cgggcggact gaccaagcgt tgtcgctggc gatcggcaca
      181 ggacgctgcc gccgcgcata ggcaccatca cgggcggcgt agctcggctc agcaaccgtc
      241 ggcgcctgcc tcgtcatccc ggcagcgggc gtggggtgtc ataggccggc cgagcgagtt
      301 tttccttgtc cgagcgcagg ccggcctatg gcgccgcgcg cccgcggtcg accggaaaac
      361 cggtgtctga ccccggtttc aggcgctgtc cgccagggcg cgggcggcgt cgatgacgcc
      421 ctggcggcgg gcgagcagca gctggctcat gatggcccgc agggcggccg gcttcaccgg
      481 cttgcgcatg atggcgaagc cggcgttgcg cacgtgctcg cgcgcctgct cgccgtagtc
      541 ggccgtgatg atcaccgccg gtacgtcgcc ccagcgggcg cgcaccgcct cgatggtctt
      601 caggccggtg tcgccgccgt cgaggtggaa gtccgccagc acgaggtcgg gcgggcaggc
      661 tgcatcctcg agcactgcca gcgcaccggg cgtatcggtc gccgtgagca ccgcgcagga
      721 ccagccctcc agcagccgcg ccataccctc gagaatgctg tgctcattgt ccacgcacaa
      781 cacccgggcc tcgtcgagcc gcgccgccgg gcgcggcatg gccggcaggc tggcgaccgc
      841 cgcctggctc ctggcgatcg gcacctcgac cgagaacacc gagccacggc cgagctcgga
      901 gcggacacgg acctcgtggc ccagcgcccg cgccatgcgc tgcacgatgg ccaacccgag
      961 ccccatgccc ttggcgccca gctcgtcgcg cggctggaag cggcggaatt cctcgtagat
     1021 gtccttctcg agatgctccg ccggaatgcc cggcccggtg tcgagcactt cgatgaccag
     1081 cgtgtcgccg cgccgccggc agccgagcag caccttgccc tggcgcgtgt aacgcagtgc
     1141 attggacagg aagttctgca ggatgcgccg caggaactgc cgatcgctgt gcaccgtgac
     1201 tcggcacggc acggtgcgga actcgatgcc gcgctcgcgc gcgatgatgc cgaactccac
     1261 gccgagggtg gccaggatgt cgtccacccg gaaatcggtg tggcgcggct ccagcgcgcc
     1321 cgcgtcgagc ttggagatgt cgagcagcgc ggtcagcagt tcctcggcgg cgcgcagcga
     1381 gctgtcgatg cggtcgaccg cgcgcaaggt gcgcgcatcg tgatcgccct ggccgagcgc
     1441 cgaggtgaac agccgggccg cgttcagcgg ctgcagcagg tcgtggctgg ccgccgcgag
     1501 gaagcgcgtt ttcgacaggt tggcggcttc ggcatcggcc ttggcgtcac gcaaggctcg
     1561 ctcgatgcgg ttgcgcagct cgatctcgct gcgcaggcgc cgattgactt cggacagctc
     1621 gcgggtgcgg tcctggacgc gctgctcgag cgtgtcatag gcctccttga ggccctgctg
     1681 ggcacgccgc cgctcggtga tgtcgtggaa cagcgcgaag aagccctgca ccttgccgtc
     1741 ggccgcgaac tgcgggatgt aggtcgcctc ggcataacgc tgccggccgt cctcgtcgag
     1801 cgtcgccacc tcgaagctct ggcgcatccc cgccagtgcc ccctccatgt gggcctcgcg
     1861 ctccaggaag gactcgcgcc cgatgatctg gtccacgcgc cgcccgcaga tttcgtcgcg
     1921 gtccagcccg atggcctgct cgtaggcctt gttgacgaag cggtagacca tgtcccggtc
     1981 gaggtaggcg atcagcaccg gcacgttgtc ggtgtagatg cgaatgttgc gctcgctctc
     2041 cctgagcgcc agctcggtgc gcttgtgctc ggtgatgtcg ctgaagctgg tgacgtaccc
     2101 cccgcccggc agcgggttga cgcgcatctc gatcaccgag ccgtcgtcgc gggtgcgctg
     2161 gaagacgtgc ggctcgccgc gccggaaatg cgccatccgc cgggcgacca tttcctcgac
     2221 gtcgcccggc ccgcagcgcc cgagctccgc gttgtagcgg atcagcgcct cgaccggcgt
     2281 gccgatgcgc agcaggtggt cggggtactt gaacaactcg gcatagcggc gattccagac
     2341 caccagccgg ttgtccgcgt cgatcacgct gatgccctcg gcgacgttct cgagcgtggt
     2401 ctccagcagg tcgcggttga agcgtgagat ctgcgaggct tcgccgacga tgctggccac
     2461 gtcctcgatc tgcaactcgg cgcccttgag cgccgataac agcaccgtgc gcgccgcgga
     2521 ggcccccatc gcgccggaca acagccgctc ggtcagatcg agaacctggg ggtcggcgcg
     2581 caggccgtcg gacagcggca gcccgcgcgc atgcaggtgc gcctcgaagg ccgccaccgc
     2641 cttctcacga ccgatgaagc gctccgccag gatgcgcatg tcgccgacct tggcggagcc
     2701 cttgaaacgc tccaggaagg gcaatgggcc gccgtgcccg ccgggcccgc cgggcccgtc
     2761 gacgaaggcc gccgcctggc gccgctccat cagccgcggc accgtgaacc aggagacgcc
     2821 gacgaaacac gcgatgttgg cgcccaggct ccagacgacg ccatgcgtga ccgggtccag
     2881 gccgtccagg ccgaacagcg cgtacgggct gagccaggcg atgccacgcg gcccggcggt
     2941 caccagctcc gccggcagcc agccggtctg cgcgaaggcg ggcaccagca gcgtccagat
     3001 ccacaccgca aaaccgacgg tcatgccggc gatcgcgccg ctgcgtgtcg cacgccgcca
     3061 gtacagcccg ccgatcatcg ccgggccgaa ctgggcggcc agcgcgaatg acagcaggcc
     3121 gatggacgcc agcgcaccgt acatgccgat gacgcgatag gccagccacg ccagcgcgag
     3181 gatgccgacg atcagcagcc ggcggatgcg gatcagcagg cgcgccaggt cggggcggct
     3241 cgagacctcc tcgccgcgca gcttcaccag ggccggcatg acgatctcgt tgcagagcat
     3301 ggtgctgcag gcgatggtgg cgacgatcac catgccggtg gcggccgaga agccgcccag
     3361 gaaggcgagc agcgccagcc cctcccggcc ggccaccatc ggcaggctga tgaggaagcc
     3421 gtcggcatcg gcgccggtgc cctgggtgat gatgccggcg gcggtgatcg gcaccacgaa
     3481 ggcggtgaac aacgccaggt acaaggggaa ggtccagcgc gccagcgcca ggtcccgcgg
     3541 gctggtgttc tccaccaccg ctacgtggaa ctgccgcggc aggcagagga tggccagcat
     3601 ggagatgacc gtgagcacca ggaagctgtg gccgatagtg gccgacgcat acagcgcggt
     3661 caccgtctcg ttggcgcgcg aggcctcgag cagcgcaccg ggaccgtcga acagcccgta
     3721 cagcacccac aggcccaccg ccaggaaggc caccagcttg acggcggact cgaaggcgac
     3781 cgccagcacc atcccgcggt ggctttcggt agagtccacc tggcgggtac cgaacaggat
     3841 ggtgaacacc gccagcaggc ccgccgccag caggccggtg tccgcggaga tgcgcggcac
     3901 cgtgtccatg cccgcgccgc cggtgatcag gctgtagctg cccgcgacgg ccttgagctg
     3961 cagtgcaata taaggaagca ctgtaacaag cgccatgacc gaagccagcg ccgcgatgcc
     4021 ctgggacttg ccgtagcgcg aggcaatgaa atcggcaatc gaggtgatgc tctgccgctt
     4081 gctgaccgcc acgatgcggg ccggcaggcg ggtcatcatc aggaacacga tgatcgggcc
     4141 caggtaaatg gtcaggaagt cccagccctt ggccgccgac atccccaccg ccccgaagaa
     4201 ggtccaggaa gtgcaataga cgccgagcgc gaggctgtag accaccggcg tggggacgta
     4261 tacgccccgc cggtgcagcc ggtcgccgac ataggcgacc aggaacagca gcacggcata
     4321 ggccagcgcc actgccggcg ggacccaggt ctccagcatg cgatctcctc ccgcccgtcc
     4381 ggccatgtgg ttataggggg ccggtccgga caggcggatg ctacccacat ccgccgccac
     4441 gcggaagcgg ccgggccggg cgactccgac taaggtcgca gggacaagcc cccggggcgc
     4501 tgctagtttc tgttgacccg acaccgggct gccgggctcg cggtcgtgag aaaactagag
     4561 aagctccgcg agggccaatc tccatctagc gggaggaaat tcagtgtctc aactcagtgc
     4621 agagaatcgc gccgaacatt ggcgtcgaac gcggaccctg atgatcattc acctggtcgt
     4681 ctggttcatt ttttcctacg tcgtgcactg gttcgccgtg ccgttgaaca acatcagctt
     4741 ccttggctgg ccgctcggct actacatggc cgcccagggc tcgctgatcg tgttcgtggt
     4801 ccagctgttc ctgttcaacc gtcagcagca cgccatcgac gtgcagttcg gcgtcgctga
     4861 agacgagtaa gggggacata tcatgggcat gcaatgggaa ggcaaatcct tcattgacaa
     4921 cctgccgaag atctacgggg tctacaccgg cgggttcctg gcgttcttcg ccctcatggc
     4981 ggtgctcgag gcctacgccg ggctcggcgc cgacacgatc ggtatcctgt tcatcgcgtt
     5041 caccatcggc atctatgcct tcatcggcat cctgtcgcgc accatggcgg tcgaggccta
     5101 ctacgtcgcg ggccgcagcg tgccgccggt gttcaacggc atggccacgg ccgcggactg
     5161 gatgtccggc gcgtccttcg tggcgatggc gggcggcgtg ttcatggccg gccacggcta
     5221 catggccttc atcgtgggct ggaccggggg ctacatcctg gtcgcgaccc tgatggcgcc
     5281 gtacctgcgc aagttcggct gctacaccgt gccggacttc atcg
//
LOCUS       DAOMPG010000166         5315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6525_length_5315_cov_4.119772, whole genome shotgun sequence.
ACCESSION   DAOMPG010000166 DAOMPG010000000
VERSION     DAOMPG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6525_length_5315_cov_4.119772"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2565
                     /locus_tag="PLS34_09250"
     CDS             <1..2565
                     /locus_tag="PLS34_09250"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRP87692.1"
                     /translation="LRGHRLAKLDPLGLVGPQPLPELEPAFHGLGPSDDDTVFATAGF
                     GGADRLSFREIRQRLADVYRGTIAAELGHITDTEERLWLQRRFEAAAAGGGLAGDDRR
                     HILERLTGAEGIERYLHTRYVGQKRFSLEGGDSMIPLLGDIIQQAGARGMDEVVIGMA
                     HRGRLNVLVNVLGKPPKELFSEFEGAYDPQVQRRSGDVKYHLGFSSDISTPGGNIHVA
                     LAFNPSHLEIVNPVVVGSVRARQDRRGDRDGSTVLPILIHGDAAFAGQGVVMETLQMS
                     QARGFAAGGTVHVICNNQIGFTTSHPQDARSTPHCSDVAKMIEAPIFHVNADDPDAVA
                     LVGRLAFDYRMKYRKDVVIDLVCYRRHGHNEADEPAATQPQMYGVVRNHPTARQIYAK
                     RLAADGVVGEDQAQAMVDAYRQGLDEGRVVSSSLGFVGNKFTVDWTRFDEVNFSQPVR
                     TRITPEQVERYAAAITRVPEEHTLHPRVERIMADRRRMAAGELPMDWGFAETMAYASL
                     LTEGYDIRLTGQDSGRGTFFHRHAVLHDQKGREPWVPLTQVDEDQRRFVVTDSLLSEE
                     AVLGFEYGYATTNPDTLVIWEAQFGDFVNGAQVVIDQFISSGEAKWGRYCGLMMFLPH
                     GYEGQGPEHSSARLERFLQLCAEHNMSVVVPSTPAQMFHLIRRQMVRNFRKPTVVLTP
                     KSLLRHKLSVSPLETMSSGRFLRMIPEIDPIDQAKVTRLVLCSGKVYFDLLEARRERE
                     LDNVAIVRLEQLYPFPIRRYAALLEDFPALREVVWCQEEPQNQGAWYQIRHRLQEPLQ
                     DRHQLFYAGRKGAAAPAAGYYQLHNQQQQALVSAALTGTEIAGRRQTSRKADRKQA"
     gene            2562..3797
                     /gene="odhB"
                     /locus_tag="PLS34_09255"
     CDS             2562..3797
                     /gene="odhB"
                     /locus_tag="PLS34_09255"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRP87693.1"
                     /translation="MSIEVKVPQLPESVADATLVAWHKSAGDKVSRDENLADLETDKV
                     VLEVPAPADGVLKEIRISDGTTVTAGEILAILEPGEAAGKPAAKAAPDNAKGQARSEA
                     EDAEQGDDDGEPGKAAGRLSPSVRRLVEEHGLDPAAIRGSGRDGRISKADVMAHLEQQ
                     PVQGKPAAKPAAAAAPRGAREDRRVPMTRMRARIAERLVDAQATAAMLTSFNEVDLGA
                     VMDIRKRYRDSFEKKHGVRIGFMSFFAKAAIEALRKFPVVNASVEGNDIIYHDYYDIG
                     IAVSTERGLMVPVLRDADLMSFADIERAIGEFAGKARDGKIGVEDLTGGTFTITNGGV
                     FGSLMSTPIINPPQSAILGMHKIQDRPMVVDGEIRIRPMMYIALTYDHRIIDGREAVQ
                     FLVSIKESLEDPARLLIQL"
     gene            3823..5250
                     /gene="lpdA"
                     /locus_tag="PLS34_09260"
     CDS             3823..5250
                     /gene="lpdA"
                     /locus_tag="PLS34_09260"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRP87694.1"
                     /translation="MSDSFDVIVIGGGPAGYPAAIRAAQNGLKAACIDDWQNYDGSRA
                     FGGTCLNAGCIPSKALLESSELFHRAKEEFAVHGIKTGGVELDLAAMQARKAGIVKQL
                     TGGIGQLFKANKVTGLQGRGKFLGEGKVEFTDPDGKQQTLTARHVILAAGSNPIELKS
                     VPFDGKQIVDSWGALEFDVVPGKLGVIGAGVIGLELGSVWQRLGAEVTVLEALDEFLP
                     MADAQIARDAARQFKKQGLDIRLGAKVQGAKTGKKGVTLSYADKNGAQELTVDKVIVC
                     VGRRPASQGLLADGTGVEVDERGFVKVDHECRTGVKNVWAVGDLVRGPMLAHKATEEG
                     VMVADLIAGRIAEVNYEVIPSVIYTAPEIAWVGKTEAQAKQAGIDYKVGTFPFAPNGR
                     AKAMEQAAGMVKLIASKRDDAIIGAHVIGPMAGELISELVVAMEFQASIEDIQRTVHA
                     HPTLAEAIHEAALAADGKALHGINK"
BASE COUNT          945 a         1753 c         1843 g          774 t
ORIGIN      
        1 ctgcgtggcc accggttggc aaagctcgat ccgctcggcc tggtcgggcc gcagcccctg
       61 ccggaactcg agccggcgtt ccatggcctc ggcccttcgg acgacgacac cgtttttgcc
      121 accgcgggct tcggcggcgc ggaccggctg agcttccgcg aaatccgcca gcggcttgcc
      181 gacgtctacc gcggcaccat cgccgcggag ctcgggcaca tcacggacac cgaggagcgg
      241 ctctggctgc agcggcgctt cgaggcggcc gcggccggcg gtggactcgc aggcgacgac
      301 cggcgccaca tcctcgagcg tctcaccggg gccgagggca tcgagcgcta cctgcatacg
      361 cgttacgtgg ggcagaagcg tttctcgctc gagggcggcg acagcatgat cccgctgctc
      421 ggcgacatca tccagcaggc cggcgcccgg ggcatggacg aggtcgtcat cggcatggcc
      481 caccgcggcc ggctcaacgt gctggtcaac gtgctcggca agccgccgaa ggaactgttc
      541 tccgagttcg agggtgccta cgacccgcag gtgcagcggc gctccggcga cgtgaagtac
      601 caccttggct tctcgtccga catcagcacg ccgggcggca acatccacgt cgcgctggcc
      661 ttcaatccat cgcacctcga gatcgtcaac ccggtggtgg tcggctcggt gcgcgcccgc
      721 caggaccggc gtggcgaccg cgacggctcg accgtgctgc cgatcctgat ccacggcgac
      781 gcggcgttcg ccggccaggg cgtggtgatg gagactctgc agatgtcgca ggcgcgcggc
      841 ttcgcggccg gcggcacggt gcacgtcatc tgcaacaacc agatcggctt caccaccagc
      901 cacccgcagg atgcgcgttc gacgccgcat tgcagcgacg tggcgaagat gatcgaggcg
      961 cccatcttcc acgtgaacgc cgatgatccg gacgcggtgg cgctggtcgg gcgcctcgcc
     1021 ttcgactacc gcatgaagta ccgcaaggac gtggtgatcg acctcgtctg ctaccgccgc
     1081 cacggccaca acgaggccga cgagccggcg gcgacgcagc cgcaaatgta cggtgtggtg
     1141 cgcaatcacc ccaccgcgcg ccagatctac gccaagcgcc tcgccgcgga cggcgtggtc
     1201 ggcgaggacc aggcgcaggc catggtggac gcctaccggc agggcctcga tgagggacgc
     1261 gtggtctcga gttcgctggg attcgtcggc aacaagttca cggtggactg gacgcgcttc
     1321 gacgaggtca atttcagcca gccggtgcgc acccgcatca cgccggagca ggtcgagcgt
     1381 tacgcggcgg cgattacgcg cgtgccggag gaacacaccc tgcatccgcg cgtcgagcgc
     1441 atcatggcgg accggcgccg catggcggcc ggcgagctgc cgatggactg gggtttcgcc
     1501 gagaccatgg cctacgccag cttgctgacc gagggttacg acatccgcct caccgggcag
     1561 gacagcggcc gcggcacatt cttccaccgg catgccgtgc tgcacgacca gaagggtcgc
     1621 gagccgtggg ttccgctgac gcaggtggac gaggaccagc gacgcttcgt ggtgaccgac
     1681 tcgttgttgt cggaggaggc cgtgctcggc ttcgagtacg gctacgccac gaccaacccg
     1741 gatacgctgg tgatctggga ggcgcagttc ggcgacttcg tcaacggcgc acaggtggtg
     1801 atcgaccagt tcatcagttc cggcgaggcc aagtggggtc gctactgcgg gctgatgatg
     1861 ttcctgccgc acggctacga gggccagggg cccgagcatt cctcggcacg gctggaacgc
     1921 ttcctgcagc tgtgcgccga gcacaacatg tcggtggtgg tgccgtccac gccggcacag
     1981 atgttccacc tcatccgccg gcagatggtg cgcaacttcc gcaagccgac cgtggtactg
     2041 acgcccaaga gcctgctcag gcacaagctg tcggtctcgc cgctggagac gatgtcgagc
     2101 gggcgcttcc tgcggatgat tcccgagatc gatccgatcg accaggccaa ggtgacgcgc
     2161 ctggtgctgt gcagcggcaa ggtctatttc gacctgctgg aggcgcggcg cgaacgcgag
     2221 ctcgacaacg tcgccatcgt gcggctcgag cagctctatc ccttcccgat ccggcgctac
     2281 gcggcgctgc tcgaggattt ccctgcgctg cgggaagtgg tgtggtgcca ggaagagccc
     2341 cagaaccagg gcgcctggta ccagatccgt catcgcctgc aggagccgct gcaggaccgc
     2401 caccagctgt tctacgcggg ccgcaagggc gcggcggcgc cggcggccgg atactatcaa
     2461 ctgcacaatc agcagcagca ggcactcgtg agcgcggcgt tgaccggcac ggagatcgcc
     2521 ggccgccgcc agacatccag gaaagctgac aggaaacaag catgagcatc gaagtgaaag
     2581 tgccccagct gccggaatcg gtggcggacg cgacgctggt cgcgtggcac aagtcggccg
     2641 gcgacaaggt gagccgcgac gagaacctcg ccgacctcga gaccgacaag gtggtgctgg
     2701 aagtgccggc accggccgac ggcgtcctca aggaaattcg catttccgac ggcacgaccg
     2761 tcacggcggg cgaaattctc gccatcctcg agcccggcga ggccgccggc aagccggccg
     2821 ccaaggcagc gccggacaac gccaagggcc aggcccggtc cgaggcggag gatgccgagc
     2881 aaggcgacga cgacggtgag cccggcaagg ccgcgggccg gttgagcccg tcggtgcgcc
     2941 ggctggtgga agagcacggg ctcgaccccg cggcgatccg tggctcgggc cgggacggcc
     3001 gcatcagcaa ggccgacgtg atggcccatc tcgagcagca gcctgtgcag ggcaagccgg
     3061 ccgcgaagcc cgccgcggcg gcggcaccac gcggcgcgcg cgaggatcgc cgcgtgccga
     3121 tgacgcggat gcgtgcccgc atcgccgagc ggctggtgga tgcgcaggcc acggccgcga
     3181 tgctgacctc cttcaacgag gtggacctcg gcgccgtgat ggacattcgc aagcgctacc
     3241 gcgacagctt cgagaagaag catggtgtgc gcatcggctt catgagcttc ttcgccaagg
     3301 ccgccatcga ggcgctgcgc aagttcccgg tggtcaatgc ctcggtggaa ggcaacgaca
     3361 tcatctatca cgactactac gacatcggca tcgccgtatc cacggagcgg ggactgatgg
     3421 tacccgtgct gcgcgacgcc gacctgatga gcttcgctga catcgagcgg gccatcggcg
     3481 agttcgccgg caaggcgcgc gacggcaaga tcggcgtgga ggacctgacc ggcggcacct
     3541 tcaccatcac caacggcggc gtgttcggct cgctgatgtc gacgccgatc atcaacccgc
     3601 cgcagagcgc catcctcggc atgcacaaga tccaggaccg gccgatggtg gtcgacggcg
     3661 agatccgcat ccggccgatg atgtacatcg cgctgaccta cgatcaccgc atcatcgacg
     3721 gacgcgaggc ggtgcagttc ctggtcagca tcaaggaaag cctcgaggat ccggcccggc
     3781 tgctgatcca gctttgacac gcattacaaa ccaggtaaga ccatgagtga cagcttcgac
     3841 gtgatcgtca tcggcggcgg gcctgccggc tatccggccg ccatccgcgc ggcgcagaac
     3901 ggcctcaagg ccgcgtgcat cgacgactgg cagaactacg acggcagccg cgcctttggc
     3961 ggcacctgcc tgaacgccgg ctgcatcccc tccaaggcgc tgctggagtc ctccgagctg
     4021 ttccaccgcg cgaaggaaga gttcgccgtg cacggcatca agaccggcgg cgtcgagctg
     4081 gacctggcgg ccatgcaggc ccgcaaggcc ggcatcgtca agcagctgac cggcggcatc
     4141 ggccagctgt tcaaggccaa caaggtcacc gggctgcagg ggcgcggcaa gtttctcggc
     4201 gaaggcaagg tggaattcac cgatcccgac ggcaagcagc agacccttac ggccaggcac
     4261 gtcatcctgg cggccggctc gaatcccatc gagctcaaga gcgtgccttt cgacggcaag
     4321 caaatcgtcg actcctgggg tgcgctggag ttcgatgtgg tgcccggcaa gctgggggtg
     4381 atcggcgccg gcgtgatcgg gctggaactc ggcagcgtct ggcagcggct cggcgccgag
     4441 gtcaccgtgc tcgaggcgct ggacgagttc ctgccgatgg cggacgcgca gatcgcacgc
     4501 gatgccgcgc ggcagttcaa gaagcagggc ctggacatcc gcctgggcgc caaggtgcag
     4561 ggcgccaaga ccggcaagaa gggcgttacg ctgagctatg cggacaagaa cggcgcgcag
     4621 gagctgaccg tggacaaggt catcgtgtgc gtcggccggc gtcccgcctc gcagggcttg
     4681 cttgccgacg gcaccggtgt cgaggtcgac gagcgcggct tcgtcaaggt ggatcacgag
     4741 tgccgcaccg gcgtaaagaa tgtctgggcg gtgggcgacc tggtacgtgg cccgatgctc
     4801 gcgcacaagg cgacggaaga gggcgtaatg gtcgccgacc tgatcgccgg caggattgcc
     4861 gaggtcaact acgaggtgat tccgtcggtg atctacaccg cgcccgaaat tgcctgggtc
     4921 ggcaagaccg aagcgcaggc caagcaggcc ggcatagatt acaaggtcgg gactttcccg
     4981 tttgcgccga acggccgcgc caaggccatg gaacaggcgg ccggcatggt gaagctgatc
     5041 gccagcaagc gtgacgacgc catcatcggc gcgcatgtca tcgggccgat ggccggcgag
     5101 ctgatcagcg agctggtggt ggcgatggag ttccaggcca gcatcgagga catccagcgc
     5161 accgtgcatg cacacccgac gctggccgag gccattcatg aggccgcact ggccgccgac
     5221 ggcaaggcac tgcacggcat caacaagtaa accggggtca gacacctgat tccggtcgac
     5281 cgcggaggcc tgccgccacg ggcgtgcttc ggttg
//
LOCUS       DAOMPG010000167         5293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6560_length_5293_cov_3.203131, whole genome shotgun sequence.
ACCESSION   DAOMPG010000167 DAOMPG010000000
VERSION     DAOMPG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_5293_cov_3.203131"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLS34_09265"
     CDS             <1..1199
                     /locus_tag="PLS34_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRP87695.1"
                     /translation="AAAVMASLAPLDTARVHAEAARPADHEARVAAFGEAARLPEASP
                     PRHLSLQINRALHDLMIKYPEMTLFGEDVARKGGVYTVTAGLAAAFRGNRVFNTLLDE
                     TTILGMAQGAAYMGLLPCPEIQYLAYFHNACDQVRGEACSLQYFSNGQWANPMVIRIA
                     GLAYQKGFGGHFHNDNSFAALRDIPGLVVACPARGDDAAGMLRTAAALAKVNGRVVAF
                     LEPIALYMTKDLYEDKDGGWLCAYPPPGEFVPFGAPRAYGPERPDLLVVTYGNGVRMA
                     LRAARRIVEDAGAGARIRVLDLRWLKPLNGAAIVAEADAAGRVLVLDEGRRTGGIAEE
                     IFTLLDERARPELPKARICGEDTYIPLGAAADTVLVSEAGAEAAMRALLARDGSRATR
                     RPAREP"
     gene            1202..2557
                     /locus_tag="PLS34_09270"
     CDS             1202..2557
                     /locus_tag="PLS34_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP87696.1"
                     /translation="MDLLDDVARRAARYLRELPARAIWPDDAALAALDKFVEPMPRPP
                     APAEAVLAMLDELGSPATVASAGPRYFGYVVGGALPSAVAANWLAAAWDQNAHHWAGS
                     PVATRLEEVALGWVTELAGLPQGTGGAFVTGASIANFCALAAARHALLGRLGWDVEAR
                     GLYGAPEIQVVVGEEVHSTVLKAIALLGLGRERVVRVPADAQGRMRADAVPEIHGPAI
                     VCLQAGNVNTGACDPMHEIIPSARAAGAWVHVDAAFGFWAAASPRYAALVEGMALADS
                     WATDAHKWLNVPYDCGLALVRDPAALRAAMSINAAYLPDEQYREPSRFGPELSRRARG
                     VEVWAALKHLGREGLAALVERCCEHAQRFAAGLTATGYTILNEVALNQVLVSFGSPAQ
                     TRRVVAALQAEGTCWCSGTEWQGHTAMRISVTSWATTAADVEQSLDAILRVAAQELER
                     G"
     gene            complement(2569..2793)
                     /locus_tag="PLS34_09275"
     CDS             complement(2569..2793)
                     /locus_tag="PLS34_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87697.1"
                     /translation="MNSPYPHRLPPGPGRSRRLPDRRRMHDLATRIGRARLVYLLGRS
                     GTPLTDWVPLGLACLVLSLLVLAGPGVPPA"
     gene            2920..3621
                     /gene="pdsR"
                     /locus_tag="PLS34_09280"
     CDS             2920..3621
                     /gene="pdsR"
                     /locus_tag="PLS34_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HRP87698.1"
                     /translation="MSQHIAIVEDEPAIRENYADAFRRAGYRVSAFAGRRAALDAFDA
                     RLPDLVVIDVSLGAEPEGGFELCRELRTRAPQLPIIFLTARDSDFDAVSGLRLGADDY
                     LTKDISLPHLLARIAALFRRVQVLTEPAGTARTLERGKLRVDAERLAAHWNEREVDLT
                     LTEFWIVHALAKHPGHVKNRQQLMDAARVVLDDATITSHVKRIRRKFEAIDSGFDAIQ
                     TVYGLGYRWRDGTAA"
     gene            3618..4052
                     /locus_tag="PLS34_09285"
     CDS             3618..4052
                     /locus_tag="PLS34_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87699.1"
                     /translation="MKLAGQLLAVSAVTLLLPWAGCQYAREIETALRDGEQEALASTA
                     HLLAAALAPTAARFDPQPERWGAGRGRGEDVFLHELANAPTLDGFVEDWGAAADAAEQ
                     FGGMSLVAGRRGAFVHLCAVAPASRSAAAWRRRRSHWRRPGP"
     gene            4049..>5293
                     /locus_tag="PLS34_09290"
     CDS             4049..>5293
                     /locus_tag="PLS34_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRP87700.1"
                     /translation="MSAVIGGAAEDPRARGYWQPTSRGWQIEARLPAALLEGRLGLRL
                     LERDGAVRGATFRTAPGWTTGRDPAAQAALDALAAPGQRVFLTDRAGFVLAQSGPRRR
                     ADPPTAAGWLDALYRGVLGARLDADAPPPARPGQLSGRHMELASLGVDDVRRFALPGG
                     GLLAAAAPIGAGGSVRAIVVVERDSAEILSLTRTPTRRLLTTSLLASFGAAAALLGYA
                     GWLSLRIRRLRDAAAAAVGTRGDIAGHLPGAGARDELGDLARSFGGLLGRVRDHNLYL
                     QSLGGRLAHELRTPLAVVRSSLDNLEAENATQGPWLGRARDGVDRMGGLVNALAAARQ
                     IERAVAAAEHQRFDLAAQVGDMVDAYRVLDPERSFVFTRPPQPCVLAGAPDLIAQLLD
                     KLVDNAVDFAPPDAPISLAIEAA"
BASE COUNT          698 a         1889 c         1985 g          721 t
ORIGIN      
        1 atgcggccgc ggtgatggcg agcctggcgc cgctggacac cgcccgggtg cacgccgagg
       61 cggcgcggcc ggctgatcac gaggcccgtg tggcggcctt cggtgaggca gcgcggctgc
      121 ctgaagcgag tccgccgcgt cacctgtcgc tgcagatcaa ccgcgcgctg cacgacctga
      181 tgatcaagta cccggagatg acgctgttcg gcgaagacgt cgccagaaag ggcggcgtct
      241 acacggtgac cgcagggctg gccgccgcct tccgcggcaa ccgcgtgttc aacaccctgc
      301 tcgacgagac caccattctc ggcatggccc agggcgcagc ctacatgggc ctgctgcctt
      361 gcccggaaat ccagtacctg gcgtatttcc acaacgcctg cgaccaggtg cgcggcgagg
      421 cctgctcgct gcagtacttc tccaacgggc agtgggccaa tcccatggtg atccgcattg
      481 cggggcttgc gtatcagaag ggcttcggcg gccactttca caacgacaat tccttcgccg
      541 cgctgcgcga catccccggc ctggtcgtcg cctgcccggc gcgcggcgac gacgccgcgg
      601 gcatgctgcg caccgcggcg gcgctggcga aagtcaacgg ccgggtggtg gcgtttctcg
      661 agcccattgc gctgtacatg accaaggacc tttacgagga caaggacggc ggctggctgt
      721 gcgcctaccc gccgcccggc gagttcgtgc ccttcggtgc gccgcgcgcc tacgggcccg
      781 aacggcccga cctcttggtc gtcacctatg gcaacggcgt gcgcatggcg ctgcgcgccg
      841 cgcggcgcat cgtagaggac gcgggcgcgg gcgcgcgcat tcgcgtgctg gacctgcgct
      901 ggctcaagcc gctcaacggc gcggccatcg tagccgaagc cgacgcggcc ggccgcgtgc
      961 tggtgctcga cgagggtcgc cgcaccggcg gcatcgccga ggagattttc acgctgctcg
     1021 acgagcgcgc gcggccggag ctgcccaagg cgcgcatctg cggcgaggac acctacatcc
     1081 cgctgggcgc ggcggccgac acggtgctgg tttccgaagc gggggccgag gcggccatgc
     1141 gggccttgct ggcccgcgac ggcagccgag ccacgcgcag gccggcgcgg gagccctgat
     1201 catggacctg ctggacgacg tcgcgcggcg ggcggcacgc tacctgcgcg aactgccggc
     1261 gcgcgcgatc tggccggacg acgctgcgct ggccgcgctc gacaagttcg ttgagcccat
     1321 gccgcggcca ccggcgccgg ccgaggccgt gctggccatg ctcgacgaac tgggctcgcc
     1381 ggcgaccgtg gcgagcgccg ggcctaggta tttcggctac gtcgtgggcg gcgcgctgcc
     1441 ctcggccgtc gccgcgaact ggctggccgc ggcctgggac cagaacgcgc accactgggc
     1501 cggctcgccg gtggcgacgc gtctcgagga ggtcgcactg ggctgggtca cggaactcgc
     1561 cggcctgccg cagggcaccg gcggcgcgtt cgtcaccggc gcttcgatcg ccaatttctg
     1621 cgcgctggcg gcggcccggc acgcgctgct cgggcggctc ggctgggacg tcgaggcgcg
     1681 cgggctgtac ggggcgccgg aaatccaggt ggtggtgggc gaggaggtcc attccacggt
     1741 gctcaaggcc attgcgctgc tcggcctggg ccgcgagcgc gtagtccgcg tgccggccga
     1801 cgcccagggc aggatgcgtg ccgatgccgt gcctgagatc cacggtcccg ccatcgtctg
     1861 cctgcaggcc ggcaacgtga acaccggcgc ctgcgatccc atgcacgaaa tcattccgtc
     1921 ggcgcgggcg gccggcgcct gggtgcacgt cgacgcggcc ttcggcttct gggcggccgc
     1981 ttcgccgcgc tacgcggcgc tggtcgaggg tatggcgctg gccgattcct gggcgaccga
     2041 tgcgcacaag tggctcaacg tgccctacga ctgcgggctg gcgctggtca gggatcccgc
     2101 cgccctgcgc gccgccatga gcatcaatgc cgcctacctg cccgacgagc agtaccgcga
     2161 accgtcgcgt ttcggaccgg agctgtcgcg ccgggcgcgc ggcgtcgagg tctgggccgc
     2221 gctgaagcac ctcggccggg aggggctcgc cgcgctggtg gaacggtgct gcgagcacgc
     2281 ccagcgcttt gccgccggcc tgacggcgac cggatatacg atcctcaacg aggtggcgct
     2341 caaccaggtg ctggtgtcct tcggcagccc ggcgcagacg cgacgggtcg ttgccgcatt
     2401 gcaggcggag ggcacctgct ggtgcagcgg caccgagtgg cagggccaca ccgccatgcg
     2461 catcagcgtc acctcctggg ccaccaccgc cgcggacgtc gagcagagcc tcgatgcgat
     2521 cctgcgcgtc gcggcgcagg agctggagcg gggctgagcc gggcccgctc aggccggcgg
     2581 cacgccgggc ccggcgagca cgagcaggct gagcaccagg cacgcgaggc ccagaggcac
     2641 ccagtcggtg agcggcgtgc cgctgcgccc cagcaggtac accaggcgcg cccggccgat
     2701 gcgcgtggcg agatcgtgca tgcgccggcg atcgggcagg cgacggctgc ggcccggccc
     2761 ggggggaaga cgatgcggat aaggcgagtt catcggtgtg ctcctggaag aacggggtca
     2821 cgcctgcatt acgcgcgcgc cgccgggcac acggcgggcg ggaccggggc gatgctcgga
     2881 tagaatgtgg cgaattctga ctgccgggac gcgccgccca tgagccagca catcgccatc
     2941 gtcgaggacg agccagcgat tcgcgagaac tacgccgacg ccttccggcg tgccggctac
     3001 cgggtcagcg ccttcgcggg gcggcgcgcg gcgctggacg ccttcgacgc ccgcctgccg
     3061 gacctggtgg tcatcgacgt cagcctcggc gccgagcccg agggcggctt cgagctgtgc
     3121 cgcgagctgc gcacgcgcgc gccgcaactg ccgatcattt tcctgactgc gcgcgacagc
     3181 gatttcgacg cggtgtccgg cctgcgcctg ggcgccgacg actacctcac caaggacatc
     3241 agcctgccgc acctgctggc gcggattgcc gcgctgttcc gccgcgtgca ggtcctgacc
     3301 gagccggccg ggaccgcgcg cacgctcgag cgaggcaagc tgcgcgtgga tgcggagcgg
     3361 ctggcggccc actggaacga acgcgaagtc gacctgacgc tcaccgagtt ctggatcgtg
     3421 cacgcgctgg cgaagcaccc ggggcacgtg aagaatcgcc agcaactcat ggatgccgcg
     3481 cgggtggtgc tcgacgacgc gaccatcacc tcgcacgtga aacgcatccg ccgcaagttc
     3541 gaagcgatcg attccggctt cgatgccatc cagacggtct acggcctcgg ctatcgctgg
     3601 cgcgacggca ccgctgcatg aaactggccg gccagctgct ggccgtcagc gcggtcacgc
     3661 tgctgctgcc ctgggcgggc tgccagtacg cgcgcgagat cgagacggcg ttgcgggacg
     3721 gcgagcagga ggcgctggcg tccacggcgc acctgctcgc tgcggcgctg gcgccgacgg
     3781 cggcccgctt cgacccgcag ccggagcgct ggggcgcggg ccgcgggcgg ggcgaggacg
     3841 tcttccttca cgagctcgcc aatgccccga cactggacgg cttcgtcgag gactggggcg
     3901 cggcggccga cgccgccgag cagttcggcg gcatgtccct ggtggcgggc aggcggggcg
     3961 ccttcgtgca cctgtgcgcc gtggcgcccg cttcgaggtc cgcggccgca tggcgacggc
     4021 ggcgttccca ctggaggcgc ccgggcccgt gagtgcggtc attggcgggg ccgccgagga
     4081 cccccgcgcg cggggctact ggcagccgac cagccgcggc tggcagatcg aggcgcgcct
     4141 gcccgcagcc ctgctggagg gccgcctcgg gttgcgcctg ctcgaacggg acggcgccgt
     4201 acgcggcgcg accttcagaa cggcgcccgg atggacgacc gggcgcgatc ccgcggccca
     4261 ggcggcgctg gatgcgctcg cggcgcccgg ccagcgcgtg ttcctgaccg atcgtgccgg
     4321 cttcgtgctc gcgcagagcg gtccgcggcg gcgtgcagac ccgccgacgg cggcgggctg
     4381 gctcgacgcc ctgtaccgcg gggtgctcgg cgcgcggctg gacgccgatg cgccgccgcc
     4441 ggcccgcccg ggccagctga gcgggcgaca catggaactg gcgtcgctgg gcgtggacga
     4501 cgtccgccgc ttcgccttgc cgggtggcgg cctgctcgcg gcggcggccc cgatcggcgc
     4561 gggcggatcg gtgcgggcga tcgtcgtcgt agagcgcgac tcggccgaga tcctcagcct
     4621 gacgcgcacg ccgacgcgac gcctgctgac caccagcctg ctcgccagct tcggcgcggc
     4681 cgctgcgctg ctcggctatg cgggctggct gtcgctgcgc attcgtcgcc tgcgggacgc
     4741 ggcggcagcg gccgtcggaa cccgcggtga tatcgccggc cacctgcccg gcgccggcgc
     4801 cagggacgag ctcggcgatc tcgcgcgcag cttcggcggc ttactcgggc gggtgcgcga
     4861 ccacaatctc tacctgcaga gcctcggcgg gcggctggcg cacgagctgc gcacgccgct
     4921 ggctgtggtg cgcagctcgc tggacaacct cgaggcagag aacgccacgc agggtccgtg
     4981 gctggggcgc gcgcgcgacg gggtggatcg catggggggg ctggtgaacg ccctcgcggc
     5041 ggcgcgccag atcgagcgcg ccgtggcggc cgcggagcac cagcgcttcg acctcgccgc
     5101 acaggtcggc gacatggtgg atgcctaccg cgtcctggac ccggaacgaa gcttcgtatt
     5161 cacgcggccg ccgcagcctt gcgtcctggc aggcgctccc gacctcattg cccagctgct
     5221 cgacaagctg gtggacaacg ctgtggactt cgcaccgccc gacgcaccga tcagcctcgc
     5281 catcgaggcg gcc
//
LOCUS       DAOMPG010000168         5281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6580_length_5281_cov_5.386529, whole genome shotgun sequence.
ACCESSION   DAOMPG010000168 DAOMPG010000000
VERSION     DAOMPG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6580_length_5281_cov_5.386529"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="atpF"
                     /locus_tag="PLS34_09295"
     CDS             complement(<1..230)
                     /gene="atpF"
                     /locus_tag="PLS34_09295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011576978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRP87701.1"
                     /translation="MGINLTLFGQMLTFLVFVWFTKKFVWPPVMQALEERRARIAEGL
                     AAAERGQKALEEADAQVAERLREARQQAAQII"
     gene            complement(284..550)
                     /gene="atpE"
                     /locus_tag="PLS34_09300"
     CDS             complement(284..550)
                     /gene="atpE"
                     /locus_tag="PLS34_09300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRP87702.1"
                     /translation="MEAIANVQAFTALAVGLIFAFAALGTGIGFGLLGGKFLEGAARQ
                     PEMANMLQIRMFIVAGLLDALAIIGVAFAALLLFANPLLAALGS"
     gene            complement(591..1355)
                     /gene="atpB"
                     /locus_tag="PLS34_09305"
     CDS             complement(591..1355)
                     /gene="atpB"
                     /locus_tag="PLS34_09305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRP87703.1"
                     /translation="MAASPTEYVTHHLEHLAVGEGFWTLHIDTLFWSWVTGLIFLGVF
                     YGAARAVTAGVPGKLQNFVETILEFVDKSVSEAFHGPKGLLAPLALTIFVWVFLWNLM
                     DLIPVDLFPVLAAMVGIEYMRVVPSADMSATFSLSLSVLVLIIYYGIKGKGAAGFGKE
                     FLFHPFGKNPLLVPFNLILNTVEMIAKPVSLALRLFGNLYAAELIFILIAMLPFWAQW
                     LPGSAWAIFHILVIPLQAFIFMTLTIVYIALAYEKH"
     gene            complement(1361..1714)
                     /locus_tag="PLS34_09310"
     CDS             complement(1361..1714)
                     /locus_tag="PLS34_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRP87704.1"
                     /translation="MAGQTGVTLVAAVVFGLAMGGEAALGALAGGGISLVLSLWMALR
                     VFSVPAEAGPQAMLSAFVKAEAIKLVMAVVLFSAAVIFLGHVFLPLVVTFVATLVVNW
                     LALIFTRRDRQGFGG"
     gene            complement(1894..2769)
                     /locus_tag="PLS34_09315"
     CDS             complement(1894..2769)
                     /locus_tag="PLS34_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRP87705.1"
                     /translation="MAVKKRSLGRGLDALLSSASRPPAAESADQGQYRELAVELLKRG
                     RYQPRLDIRQESLEDLADSIRAQGVVQPIVIRPVRDTGDGVRYEIIAGERRWRAAQLA
                     GLQKVPVIVRDVPDEAAVAMALIENIQREDLNPLEEARSLDRLIREFEMTHQQAAEAV
                     GRSRAAVSNLLRLLELGDEVKALVEKRAIEMGHARALLGLADPRAQAAAAREVASKGL
                     SVRETERLVKRLQSPPAKAPARKPDANIRRLEAELADKLGAKVALQHAASGAGKLVVS
                     YNSLDELDGILAHIR"
     gene            complement(2847..3626)
                     /locus_tag="PLS34_09320"
     CDS             complement(2847..3626)
                     /locus_tag="PLS34_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRP87706.1"
                     /translation="MTRILAIANQKGGVGKTTTAVNLAASLAATRRRVLLVDLDPQGN
                     ATMGSGVNKSELELSSCDVLLGEAAARDAIVRTDPGGFDLIPGNADLTTAEVRLISEF
                     GRETRLRTALKDVVADYDYVLIDCPPSINMLTVNALTAADGVLIPMQCEYYALEGLSA
                     LVSTIEQIRDAVNPGLQIEGLLRTMFDPRNNLANEVSSQLILHFGDKVFRTVIPRNVR
                     LAEAPSFGMPALFHDKTSRGALAYLALAGEMIRRRAAAAVG"
     gene            complement(3623..4267)
                     /gene="rsmG"
                     /locus_tag="PLS34_09325"
     CDS             complement(3623..4267)
                     /gene="rsmG"
                     /locus_tag="PLS34_09325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRP87707.1"
                     /translation="MDESRVQQLIEQGLAELDLREPPGLAAALAGYLELLARWNRTYN
                     LTAVREPEEMVTRHVLDSLVVLPFIDAERMLDVGTGAGLPGLVLAMARPGQRFVLLDS
                     SGKKTRFVEHAATRLGLGNVEVVQARVEDHEDEEGFAVVLSRAFASVADFIRLAGHLA
                     RTEGRLLAMKGALPEAELAALPAGWGLQAVHKLSVPGLAGRRHLLEFCRKDNRT"
     gene            complement(4313..>5281)
                     /gene="prpD"
                     /locus_tag="PLS34_09330"
     CDS             complement(4313..>5281)
                     /gene="prpD"
                     /locus_tag="PLS34_09330"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HRP87708.1"
                     /translation="LENSFNRVGLDHVLLVRIASTAVATRMLGGDREQVINAVSNAWL
                     DGAALRAYRHAPNTGSRKSWAAGDATSRGVRLALMALAGEMGYPSALTAERWGFQDVL
                     FGGRPLALPQDFGTYVMENVLFKISYPAEFHAQTAVEAAMKLHPEVRERLDDIREIVI
                     ETQEPALRIIDKTGPLANPADRDHCLQYMTAIPLIFGRLTASDYEDAVARDPRVDALR
                     ERMTVRENERFTRDYYDPAKRYIGNAVQVFFRNGSSTERIEAEVPVGHRKRRAEGIPL
                     LVRKFENSLKGKLTDAQFKGLKSLCADQEKLEATPVDDFVALLVAP"
BASE COUNT          872 a         1808 c         1777 g          824 t
ORIGIN      
        1 tcgatgatct gcgccgcctg ctggcgggct tcccgcaggc gctccgccac ctgggcgtcg
       61 gcttcctcga gcgccttctg gccgcgctcg gcggcggcaa ggccctcggc gatgcgcgcc
      121 cggcgctctt ccagcgcctg catgaccggc ggccaaacga acttcttggt gaaccagacg
      181 aagaccagga aggtcagcat ctggccgaag agtgtcaggt taatgcccac gattccgact
      241 cccgatcagc gtgcgtttgc ttgcgaagac gccatgcgtc ccttcagctc ccgagcgcgg
      301 cgagcagcgg attggcgaac agcagcagcg cggcgaaggc gacgccgatg atggcgagcg
      361 cgtcgagcag gccggccacg atgaacatgc ggatctgcag catgttggcc atttccggct
      421 ggcgcgcggc gccctcgagg aacttgccgc cgagcaggcc gaaaccgata ccggtgccga
      481 gcgcggcgaa cgcgaagatg agtccgacgg ccagcgcggt gaaggcctga acgtttgcaa
      541 ttgcttccat gatcaatctc cgtaggaggc agtttctaca ggggtgaaaa tcagtgcttc
      601 tcgtaagcca gcgcaatgta gacgatggtc agcgtcatga agatgaacgc ctgcagcggg
      661 atgacgagaa tgtggaagat cgcccaggcg ctgccgggga gccactgggc ccagaacggc
      721 agcatggcga tcaggatgaa aatcagctcg gcggcgtaca ggttgccgaa caggcgcaac
      781 gccagcgaga ccggcttggc gatcatctcg acggtgttga gaatcaggtt gaagggcacc
      841 agcagcgggt tcttgccgaa ggggtggaac aggaactcct tgccgaagcc ggcggccccc
      901 ttgcccttga tgccgtaata gataatgagt acgagcaccg acagcgacag gctgaaggta
      961 gcgctcatgt cggccgacgg cacgacgcgc atgtactcga tgcccaccat cgcggcgagc
     1021 accgggaaca ggtccaccgg gatcaggtcc atgaggttcc agaggaacac ccacacgaag
     1081 atagtcagcg ccagcggcgc aagcagcccc ttcgggccgt ggaaggcttc gctcaccgac
     1141 ttgtcgacga attcgaggat ggtctcgacg aagttctgca gcttgcccgg tacgccggcg
     1201 gtgaccgccc gcgccgcgcc gtagaacacg ccgaggaaga tcagcccggt gacccaggac
     1261 cagaacagcg tgtcgatgtg gagcgtccag aagccctcgc cgacggcgag atgctcgagg
     1321 tgatgcgtga cgtattcggt cggagaagct gccatttccg tcaaccacca aagccctgtc
     1381 ggtcacgtcg cgtgaaaatc agcgccagcc agttcacgac cagcgtggcg acgaacgtga
     1441 ccacgagcgg taagaacacg tggcccaaaa aaatgactgc ggctgagaac agcacgacag
     1501 ccatcaccaa cttgattgcc tccgccttga cgaaggccga gagcatcgcc tggggtcccg
     1561 cctcggccgg aaccgagaaa acccgcagtg ccatccagag actcagcacg aggctgattc
     1621 cccctcccgc cagtgctccg agagcggcct cgccgcccat cgcgagcccg aacacgaccg
     1681 cggcgaccaa ggtcacgccc gtctgtccgg ccaagatctt tccgggtact gaaccgaccc
     1741 gttgtcgcat gccgagggac ctcggcccca aaagccgcag gattgtatcc cgtttccgca
     1801 gtcgcagcaa tttcggaaaa tgccgctgaa ttggggacat tccccttttc cgcgtggccg
     1861 aaaaggggaa tgtccccaat tcagcggccc gattcagcgg atgtgggcga ggatcccgtc
     1921 cagctcgtcg aggctgttgt agctgacgac cagcttgccg gcgccggacg cggcatgctg
     1981 gagggcgacc ttggcgccga gcttgtcggc cagctcggcc tcgaggcgcc ggatgttggc
     2041 gtcgggcttg cgcgccggcg ccttggcggg cggcgactgc agccgcttca ccagccgctc
     2101 ggtctcgcgg accgacaggc ccttggacgc cacttcgcgc gccgccgcgg cctgcgcccg
     2161 cgggtccgcc agcccgagca gcgcccgggc gtggcccatc tcgatggcgc gcttctccac
     2221 cagcgccttg acctcgtcgc ccagctccag cagccgcaac agattgctga ccgcggcgcg
     2281 cgagcggccg accgcctccg cggcctgctg gtgggtcatt tcgaactcgc ggatcagccg
     2341 gtccagcgag cgcgcttcct cgagcgggtt gaggtcctcg cgctggatgt tctcgatcag
     2401 cgccatcgcc acggccgcct cgtcgggcac gtcgcgcacg atcaccggca ccttctgcag
     2461 gcccgccagc tgggcagcgc gccagcggcg ctcgccggcg atgatctcgt agcggacacc
     2521 gtcgccggtg tcgcgcaccg ggcggatgac gatcggctgc accacgccct gcgcacggat
     2581 cgagtcggcg aggtcctcca gcgattcctg gcgaatgtcg aggcgcggct ggtagcggcc
     2641 ccgcttgagc agctcgacgg ccagctcgcg gtactgtccc tggtcggccg actccgcggc
     2701 cggcgggcgg ctggcggatg acagcagggc gtcgaggccg cggcccagcg agcgtttctt
     2761 gacggccatc aggcttcttt ctccgtaatc gttcccggcg gggtcagcgc ccggaagtgt
     2821 accgcaagcg cctgccgccg gcggactcag cccacggccg cggccgcccg gcggcggatc
     2881 atctcgccgg ccagcgccag gtaggccagc gcgccgcggc tggtcttgtc gtggaacagc
     2941 gccggcatgc cgaagctggg cgcctccgcg agccgcacgt tgcgcgggat caccgtgcgg
     3001 aacaccttgt cgccgaaatg caggatcagc tgcgaggaga cctcgttcgc caggttgttg
     3061 cgcggatcga acatggtccg cagcaggccc tcgatctgca ggccgggatt cacggcgtcg
     3121 cggatctgct cgatggtgct gaccagcgcc gaaagcccct cgagcgcgta gtactcgcac
     3181 tgcatcggga tcagcacgcc gtccgcggcc gtcagggcgt tgaccgtcag catgttgatg
     3241 gacggcggac agtcgatgag cacgtagtcg tagtccgcga cgacgtcctt gagcgcggtg
     3301 cgcaggcgcg tctcgcggcc gaactcgctg atcagccgga cctcggccgt ggtcaggtcg
     3361 gcattgccgg gaatcaggtc gaagccgccg ggatcggtgc gcacgatggc gtcgcgcgcg
     3421 gctgcctcgc cgagcagcac gtcgcaagac gagagctcca gctcgctctt gttgaccccg
     3481 gagcccatgg tggcgttgcc ctgcgggtcg aggtccacca gcagcacgcg ccgccgcgtc
     3541 gccgccagcg aggcggcgag gttgacggcg gtggtcgtct tgccgacgcc gcctttctgg
     3601 tttgcgatgg cgagtatcct ggtcacgtcc ggttgtcctt gcgacagaat tccagcaggt
     3661 ggcggcgtcc ggccaggccg ggcacagaaa gcttgtgcac cgcctgcagt ccccagccgg
     3721 ccggcagcgc ggccagctcc gcctccggca gcgcgccttt catggccagc agccggccct
     3781 cggttcgcgc caggtggccg gccagccgga tgaaatccgc caccgaggca aaggcccgac
     3841 tcagcaccac cgcgaaacct tcttcatcct cgtggtcctc gacgcgggcc tgcacgacct
     3901 cgacgttgcc gagccccaga cgcgtcgccg cgtgctcgac gaagcgcgtc ttcttgcccg
     3961 acgagtcgag cagcacgaag cgctggccgg gccgggccat cgccagcacc aggcccggca
     4021 gcccggcgcc cgtgccgacg tccagcatcc gctccgcgtc gatgaacggc aacaccacca
     4081 ggctgtccag cacgtggcgg gtgaccatct cctcgggctc acgcacggcc gtcaggttgt
     4141 aggtccggtt ccacctcgcc agcagctcca ggtagccggc cagcgcggcg gccagcccgg
     4201 gcggctcccg gaggtccagt tccgccaggc cctgttcgat cagctgctga actcggctct
     4261 cgtccaccgg gacccgcctc cgtcggccgg ccagtataac ggcgcgacgc ggctacgggg
     4321 cgaccagcag ggccacgaaa tcgtccaccg gcgtggcctc cagcttctcc tggtcggcgc
     4381 acagcgattt caaccccttg aactgggcgt ccgtgagctt gcccttgagg ctgttctcga
     4441 acttgcggac caagagcggg atgccctcgg cgcggcgctt gcggtgcccg accggaacct
     4501 ccgcctcgat ccgctcggtg gagctgccgt tgcggaagaa cacctgcacg gcattgccga
     4561 tgtagcgctt ggccgggtcg tagtagtcgc gcgtgaagcg ctcgttctcc cgcacggtca
     4621 tccgctcgcg cagtgcgtcc acgcgcgggt cgcgtgccac ggcgtcctcg tagtccgacg
     4681 ccgtgagccg gccgaagatc agcgggatgg cggtcatgta ctggaggcag tggtcgcggt
     4741 cggccgggtt ggccagcggg ccggtcttgt cgatgatgcg cagcgcaggc tcctgggtct
     4801 cgatgacgat ttccctgatg tcgtcgagcc gctcgcgtac ctccggatgc agcttcatgg
     4861 ccgcctcgac ggcggtctgg gcatgaaact cggccgggta ggaaatcttg aacagcacgt
     4921 tctccatcac gtaggtgccg aagtcctgcg ggagcgccag gggccggccg ccgaacagca
     4981 cgtcctggaa accccaacgc tccgccgtca gggccgacgg gtagcccatc tcacctgcca
     5041 gcgccatcag ggcgaggcgc acgccgcggc tggtcgcgtc gcccgccgcc cagctcttgc
     5101 gcgagccggt gttcggcgcg tggcggtagg cgcgcagcgc agcaccgtcg agccaggcgt
     5161 tggacaccgc gttgatgacc tgctcgcggt cgccgccgag catgcgggtc gccaccgcgg
     5221 tggaggcgat gcgcaccagc agcacgtggt cgagcccgac gcggttgaag ctgttctcca
     5281 g
//
LOCUS       DAOMPG010000169         5273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6600_length_5273_cov_4.245688, whole genome shotgun sequence.
ACCESSION   DAOMPG010000169 DAOMPG010000000
VERSION     DAOMPG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6600_length_5273_cov_4.245688"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLS34_09335"
     CDS             <1..479
                     /locus_tag="PLS34_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRP87709.1"
                     /translation="IFSSILTLTVGDKSQPCILKDVQRHPYRNLLVHVDLQRIIEDEE
                     IRVSVPLHFSGEKVAPGVKAGGVISRVMNELEISCLPKYLPEFIEVDVSAVELEGMLP
                     LSAVKLPEGVAIVGGDEMLEQPVFMIHRPRLEEPEEGEGEAEAGGQESVEPEPRED"
     gene            563..1195
                     /gene="pth"
                     /locus_tag="PLS34_09340"
     CDS             563..1195
                     /gene="pth"
                     /locus_tag="PLS34_09340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRP87710.1"
                     /translation="MTERSLIRMIVGLGNPGPEYAPTRHNAGFWLADLLADRHGGTFR
                     GERKFGADLASVRIDGADVLLLKPQSFMNRSGGPVQAAAAYLKVPVEQILVAHDDLDL
                     PPGDVRLKQGGGHGGHNGLRNMITHLGPGFWRLRLGIGHPGHRAEVIDYVLQRPSRED
                     DLLIHAAVSDAADVMPILLGSGEQPAMHKLHSRSVPPRPDRNDSGPGPAE"
     gene            1200..2291
                     /gene="ychF"
                     /locus_tag="PLS34_09345"
     CDS             1200..2291
                     /gene="ychF"
                     /locus_tag="PLS34_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRP87711.1"
                     /translation="MGIRIGIVGLPNVGKSTLFNALTRAQIAAENYPFCTIDPNVGVV
                     PVPDPRLQALAAIAKPQKVLPTTIEFVDIAGLVRGASKGEGLGNQFLANIRETDAIGH
                     VVRCFEDPDVVHVEGRIDPAGDIEVINLELALADFDTVTRNLERAVKAAKTGKKEDIV
                     RRDLLAQLADHLDQGLPVRSMTLDAAQREIVRELHLLTAKPTLYIANVDEGGFHDNPH
                     LDTVTQIAAAEGAEVVPVCAAIEAEIAQLDEADKGEFLAELGLEEAGLDRVIRAGYRL
                     LGLQTYFTVGPKEVRAWTVRVGATAPEAAGEIHTDFQRGFIRAEVIAYADFIACGGES
                     KAKDTGKMRVEGKEYVVKDGDVMHFRFNV"
     gene            complement(2301..3008)
                     /locus_tag="PLS34_09350"
     CDS             complement(2301..3008)
                     /locus_tag="PLS34_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP87712.1"
                     /translation="MSHCCGSCPLPAVCGDGPDLPGALLDSVLEQRELAPGATLYRAG
                     QKRCSIWVISQGALKTCELDIEGREQVVGFHGHGDVLGLERIEIPQHRGFALALEPTS
                     VCRIPVNRLVERLTVMPALWRDVLGIAGRQIAQAREVHRVLGQLQTGQRLAWFLLEGA
                     GPRRGECGCSGDRLIHLPMQRQDIASFLGMTLETVSRSFSALQREGLIEVHGRTVRLL
                     DPAALAARIMPQEKAAA"
     gene            complement(3005..3439)
                     /locus_tag="PLS34_09355"
     CDS             complement(3005..3439)
                     /locus_tag="PLS34_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRP87713.1"
                     /translation="MRLTYFSDYAFRVLMYLSVTPDRRCTIREISEAYKISRAHLMKV
                     VNLLTREGFITAQRGPNGGLTLALPAEQIRLGDVVRKTEDDLGLVECFRPENTCRLTP
                     SCALAPALSRAMHAFLAELDKQTIADLVRRPQPIRRLLDISP"
     gene            complement(3553..3945)
                     /locus_tag="PLS34_09360"
     CDS             complement(3553..3945)
                     /locus_tag="PLS34_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HRP87714.1"
                     /translation="MYYWVKQVHMFSVLVSFTLFLARGAWVLAGRSLPRHPLLRALPH
                     TVDTVLLTSALWLTTIIQQYPFSHGWLTVKVVLLLVYIVLGSLALRRAPTPAGRAAAF
                     VGAVVTFLFLYSVARFRHPMGVLAPLLG"
     gene            complement(3959..5173)
                     /locus_tag="PLS34_09365"
     CDS             complement(3959..5173)
                     /locus_tag="PLS34_09365"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRP87715.1"
                     /translation="MSAERVDRWAVLTSAPHRMFFATGLAWLAAWSAWWMLVLGARAA
                     GIDGLEPARPALLLHGAVMLFLVLPPFMYGFLLTVFPRWMPAPPPGRAPMLAAWWLLN
                     AGNLALLAGLAGPAPLQLAGWLSAALALAVIATTLLRILLGATERASHAFAVLAGLCA
                     GLAGMLLYTPVLASGDFSTWPLVRGIGLWGFLLVVYFGVSHRMIPFFTSRVVRDYVPW
                     RPAWILYLFVACALLRAGLELAPAWAWLGSVPLAGIATACALRWRPRQRSGVRLLEVL
                     HISLAWLAIGLLLAAAADLAAALGAPGVVGRAPLHAIGMGFIGGMLMAMVTRVTLGHS
                     GRPLVMDALNWRLFLVVQAATVLRIAGEFLPGAGTMLSAIAALAWAAAFGTWTVRHLP
                     IYLRPRADGAPG"
BASE COUNT          923 a         1768 c         1869 g          713 t
ORIGIN      
        1 cgatcttctc cagcatcctg acgctcacgg tgggcgacaa gtcccagccg tgcatcctca
       61 aggacgtcca gcggcatccc taccggaacc tgctggtgca cgtcgacctg cagaggatca
      121 tcgaggatga ggagattcgc gtgtccgtgc cgctgcattt cagcggcgag aaggtggcgc
      181 cgggcgtgaa ggccggcggc gtgatttcgc gcgtcatgaa cgagctggaa atcagctgcc
      241 tgccgaagta cctgcccgag ttcatcgagg tggacgtctc ggccgtagag ttggagggca
      301 tgttgccgct gtcggcggtc aagctgcccg agggcgtggc gatcgttggc ggcgacgaga
      361 tgctcgagca gccggtgttc atgatccaca ggccgcgcct cgaggagccg gaagagggcg
      421 aaggggaagc cgaggccggt gggcaggagt ccgtcgagcc cgagccccgc gaggactgag
      481 gccgactcgt cactgcctgg cgcagggccg tccgtcggga cggccctgcg atttcgccgc
      541 cgagcggcgg catggttgaa aaatgacaga gcgctccctg atccgcatga tcgtcggcct
      601 gggcaatccg gggccggagt atgcgcctac gcggcacaac gccgggttct ggctcgcgga
      661 cctgctggcg gatcgccatg gcgggacatt tcggggcgag cgcaagttcg gcgcagacct
      721 cgcatcggtc aggatcgacg gcgccgacgt gctgctgctc aagccgcaga gtttcatgaa
      781 tcgcagcggc ggacccgtgc aagccgcggc tgcgtacctg aaggtgccgg tggagcagat
      841 cctggtcgcc cacgacgacc ttgacctgcc gcccggcgac gtacggctca agcagggtgg
      901 cggccatggc ggccataatg gcctgcgcaa catgattacc cacctcgggc cgggtttctg
      961 gcgcttgcgg ctcggcatcg gtcatccggg ccaccgcgcc gaggtgatcg actacgtgct
     1021 gcagcgtccg tctcgcgagg acgatctgtt gatacacgcc gcggtgtctg acgcagccga
     1081 tgtcatgccg atcctgctgg ggagcggcga gcaacccgcc atgcacaagc ttcatagccg
     1141 cagcgtgccg ccgcggcccg accgaaacga cagcgggccc ggtcccgcgg agtaaaagca
     1201 tgggaatccg tatcgggatc gtcggcctgc cgaacgtggg caagtccacc ctgttcaacg
     1261 cgctcacccg ggcgcagatc gcggccgaga actacccgtt ctgcaccatc gacccgaacg
     1321 ttggcgtcgt gccggtgccg gatccgcgcc tgcaggcgct ggccgccatc gcgaagccgc
     1381 agaaggtgct gcccaccacc atcgagttcg tcgacatcgc cgggctggtg cgcggcgcct
     1441 ccaagggcga gggcctcggc aaccagttcc tcgccaacat tcgcgaaacc gacgccatcg
     1501 ggcacgtggt gcgctgcttc gaggatcccg acgtggtcca cgtcgagggg cgcatcgatc
     1561 ccgccggcga tatcgaagtc atcaacctcg agctggcgct ggctgacttt gacacggtca
     1621 cgcgcaatct cgagcgggcg gtgaaggcgg ccaagaccgg caagaaggaa gacattgtcc
     1681 gccgcgacct gctcgcccag ctggccgacc atctcgacca gggcctgccg gtccgatcga
     1741 tgacgctgga tgcggcgcag cgcgagatcg tgcgcgagct ccacctgctg accgcaaagc
     1801 ccacgctgta catcgcgaat gtcgacgaag gcggctttca cgacaatccg catctcgaca
     1861 ccgtgaccca gatcgccgcc gccgagggtg ccgaagtagt gccggtgtgt gccgcgattg
     1921 aggcggagat cgcgcagctc gacgaggccg acaagggcga gttccttgcc gagctcgggc
     1981 tggaggaggc cggcctcgac cgggtgatcc gggcgggcta tcgcctgttg ggcctgcaga
     2041 cctactttac ggtcggccca aaggaagttc gcgcgtggac cgtcagggtc ggcgcgacgg
     2101 cgcccgaagc agctggtgaa attcacaccg acttccagcg tggcttcatt cgcgccgagg
     2161 tcatcgccta cgcggacttc atcgcctgcg gcggcgagtc gaaggcgaag gataccggca
     2221 agatgcgcgt cgaaggcaaa gaatacgtcg tcaaggacgg cgacgtcatg cacttccgct
     2281 tcaacgtctg agcgtcgcgc tcaggcggcg gccttctcct gcggcatgat gcgcgccgcc
     2341 agcgccgccg ggtccagcag ccgtacggta cggccgtgta cttcgatcag gccttccctc
     2401 tgcagcgcgc tgaagctccg gctgacggtc tcgagcgtca tgccgagaaa cgatgcgatg
     2461 tcctggcgct gcatcggcag gtggatcaag cggtcgccgc tgcagccgca ctctccacgg
     2521 cgcgggccgg cgccctccag caggaaccag gccagtcgct ggcccgtctg caactggccg
     2581 agcacccgat gcacctcgcg ggcttgcgcg atctgtcgtc cggcgatgcc gagcacgtcg
     2641 cgccacagcg cgggcatgac ggtgagccgt tcgaccaggc ggttgaccgg gatgcggcag
     2701 acgctggtgg gctcgagcgc cagggcgaag ccgcggtgct ggggaatctc gatgcgctcg
     2761 aggccgagta cgtcgccgtg gccgtggaag ccgaccacct gctcgcgacc ctcgatgtcc
     2821 agctcgcagg ttttcagcgc gccctgcgag atgacccaga tggaacagcg cttctggccg
     2881 gcccggtaga gagttgctcc cggggccagc tcccgttgct cgaggacgga atccagcagc
     2941 gctccgggca ggtcggggcc gtcgccgcag acggccggca gcggacagct gccacagcag
     3001 tggctcatgg cgagatgtcc agcagccggc ggatcggctg cggccggcgc accaggtcgg
     3061 cgatggtctg cttgtcgagc tccgccagga aggcgtgcat ggcgcgactg agcgccgggg
     3121 ccagcgcgca ggagggcgtc aggcgacagg tgttctccgg ccggaaacac tccaccaggc
     3181 cgaggtcgtc ttccgtcttg cgaacgacgt cgccgaggcg gatctgctcg gccggcaggg
     3241 ccagcgtcag gccgccgttc gggccgcgct gggcggtgat gaatccctcg cgggtcaaca
     3301 ggttgactac cttcatcagg tgggcgcgcg aaatcttgta ggcctcggaa atctcgcgga
     3361 tcgtgcagcg ccggtccggc gtcaccgaca ggtacatcag gacgcggaag gcgtagtcgc
     3421 tgaaataggt caggcgcatg gtcgtgcatc cgggcgccgc agcgggccct tgtattgatg
     3481 catgatacat gaactatttt gcactgcaag ccggccggcc ggctggccgg ccgcccggcc
     3541 aggacggcgg cttcaaccca gcagcggcgc aagaactccc atggggtggc gaaaccgtgc
     3601 aaccgagtac aggaacagga aggtgaccac ggcgccgacg aaggcggcag cgcggcccgc
     3661 cggcgtgggc gcccggcgca gggcaaggct gcccagcacg atgtagacca gcaacaggac
     3721 gaccttgacg gtcagccagc catggctgaa cggatattgc tggatgatgg tggtcagcca
     3781 cagggcgctg gtcagcagca ccgtgtccac ggtgtggggc agtgcccgca acaggggatg
     3841 gcgcggcagg ctgcggcctg ccagcaccca tgccccgcga gccaggaaca gcgtgaagga
     3901 caccagcacg ctgaacatgt gcacctgctt gacccagtag tacatcaagt ccgcatcctc
     3961 agccgggtgc gccgtcggcg cggggccgca ggtagatcgg caggtgccgg accgtccagg
     4021 tcccgaatgc cgccgcccag gcgagcgcgg cgatggcgct cagcatcgtg ccggcgcccg
     4081 gcaggaactc gccggcgatg cgcagcaccg tggcggcctg gacgacgaga aacaaccgcc
     4141 agttgagcgc gtccatcacc agcggccggc cggagtggcc gagagtcacg cgggtcacca
     4201 tggccatcag catgccgccg atgaagccca tgccgatggc gtgcagcggc gcgcgtccga
     4261 ccacgcccgg cgcccccagg gcggccgcga ggtcggcggc tgcagccagc agcaggccga
     4321 tcgccagcca ggccagcgag atgtgcagca cctccagcaa gcgcacgccg ctgcgttgcc
     4381 ggggccgcca gcgcagcgcg caggcggttg cgatgcctgc cagcgggacg gagcccagcc
     4441 aggcccaggc cggggcgagc tcgaggccgg cccggaggag ggcgcaagcg acgaacaggt
     4501 acaggatcca ggcaggacgc caggggacgt agtcgcgtac gacccgcgaa gtgaaaaagg
     4561 gaatcatccg gtgactgacg ccgaagtaca ccaccagcag gaagccccac aggccgatac
     4621 cgcgcaccag cggccaggtc gagaaatcgc cgctggccag caccggcgtg tacagcagca
     4681 tgcctgccag gccggcgcac aggccggcca ggaccgcgaa ggcatgcgac gcgcgctccg
     4741 tggcgcccag caggatacgc aacagggtgg tggcgatgac ggccagcgcc agcgcggccg
     4801 acagccagcc ggccagctgc agcggcgccg gccccgccag gcccgccagc aaggcgaggt
     4861 tgccggcgtt gagcagccac caggccgcaa gcatgggcgc ccggcccggc ggcggcgccg
     4921 gcatccagcg ggggaacacg gtcagcagga agccgtacat gaagggcggc agcaccagga
     4981 acaacatgac cgccccatgc agcagcagcg cgggccgggc cggctccagt ccgtcgatgc
     5041 ccgccgcgcg ggcgccgagc accagcatcc accacgcgga ccaggcggcc agccaggcca
     5101 gcccggtggc aaagaacatc cggtggggcg cggaggtcag caccgcccag cggtcgacgc
     5161 gttcggcgct catgccttgc cgcccaccgc cggatggcct ggcgggttcg ccccgccgtc
     5221 acgcgggcga cggggcgaac ggcgactcac tcccgggcct ccgcaacggt cgt
//
LOCUS       DAOMPG010000170         5270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6607_length_5270_cov_3.524640, whole genome shotgun sequence.
ACCESSION   DAOMPG010000170 DAOMPG010000000
VERSION     DAOMPG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_5270_cov_3.524640"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLS34_09370"
     CDS             <1..1192
                     /locus_tag="PLS34_09370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRP87716.1"
                     /translation="LLQAAVSRQREKLADAAAVQFTRNPDGLANALKRIAASPLSSSV
                     RAVRREEIGHMLFASGRRSLAGLMATHPPVEERIRALDPNWRPGDPPPPLPTPVEPAE
                     TAAEAARKERIRQGALPGLPGGLGLPGLDPVAAAAMIGVVDLAMAERLLAMVPEAIHQ
                     AVLEPEGAAVVALAVLLDSGERLRAHQLAMLEARLGSTVADRIAQAAGRLADVSPTLR
                     LALLDLASPALRHLPEARREDLLHLAQRLVEIDGVVTPQEAALAAALESRLSPEADRQ
                     PDPEGALAMLALLARAGHEDDAEAEQAWRDGAARLAAAGFPLPSPLPEFPATEGSAGA
                     ESAAHAPRLAAMAERHRQALLAALAEVAAHDGVIRPAELQLLRAAAAALDCPMPPLNV
                     PPSG"
     gene            complement(1189..2313)
                     /locus_tag="PLS34_09375"
     CDS             complement(1189..2313)
                     /locus_tag="PLS34_09375"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRP87717.1"
                     /translation="MVMPSLLAAWLVAPTGHAAAEKVAEGLRTEYEPIRVVRIADGLE
                     HPWAVAVLPDGRFLVSERSGRLNLLADGVKTRVAGLPEIHAHRQGGLLDVVLHPDFTA
                     NGWVYITYSKGDAAGTTTALGRGRLEGDALVGFEELFVQDRRSEPGRHYGSRIAWLPD
                     GTLLMSIGDRGASPPRAQDLRDHAGKVLRLNADGSVPNDNPFVGRDDAHAEIFSTGHR
                     NIQGMVVDAAGGAIWATEHGERGGDELNRVQAGKNYGWPVVSKARQYRSEEQYGEARS
                     RAGYVDPVYEFLPTLAPSGLALVTKDRFPSWQGNLLAGGLRAQRIRRLVIEDGEVVHE
                     EELLLQTVGRIRDVREGPDGHIYVLSDEADGGLYRIESER"
     gene            complement(2349..3377)
                     /locus_tag="PLS34_09380"
     CDS             complement(2349..3377)
                     /locus_tag="PLS34_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRP87718.1"
                     /translation="MKLQQLRYLRAVIDNGLNMTQAAERLATSQPGVSKQLKLLEQEL
                     GLSLFVRKGRSLVSLTPEGEEVAQRAVRILREVEAIRGISPNIYSEVSGRLSIGTTHT
                     QARYVLPDVIACFRAQYPEVTLDLHQGTSEQLAEMAEQRRVDFVIASESRHRFPELVV
                     LPCFRWDRIVLVPEGHALASLRRPPTLEELAAHPLVTYVFNFDGGSSLKRAFAARGLE
                     PNVAFTARDADVIKTYVARGLGIGIVASMAWSADARDGLVALDASGLFPRCTTWIGFR
                     RDVVLRKYMFDFFAMFAAHLDERTVRRMLACDSQEEVDAAMARVALPLRGSQPHGGGP
                     PQELGEGL"
     gene            3530..4486
                     /gene="cysK"
                     /locus_tag="PLS34_09385"
     CDS             3530..4486
                     /gene="cysK"
                     /locus_tag="PLS34_09385"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRP87719.1"
                     /translation="MIHDSILGTIGDTPVVRLNRIGPDHVTIYVKIESFNPMASVKDR
                     LAFAIIDDAEKRGLLKPGQTVIEATSGNTGIALAMVCAARGYPFVATMVETFSIERRR
                     VMKALGAKVILTPAAERGSGMVRRAEELAKQHGWFLARQFENEANPAFHRQTTGPEIL
                     KDFAGRRLDYWVTGWGTGGTLTGAGEVIKLARPEVKIVATEPEGASLLGGKEWKPHKI
                     QGWTPDFVPQVLNREVYDELVTVTDEESREWARALATREGILVGLSAGGTLAAALKVA
                     ERAAPGSVLLAMLPDTGERYLSTFLFEGIDESSDDAWLAEQG"
     gene            4579..5169
                     /locus_tag="PLS34_09390"
     CDS             4579..5169
                     /locus_tag="PLS34_09390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRP87720.1"
                     /translation="MARRIVIVQGHPDPGGGRYGHALAEAYREGAAGAGHEVEVIPVA
                     ELDFPLLRSKADFDGGTPPAAILRCQQLILQADHLVIIHPLWLGSMPALLKGFLEQVF
                     RPGFAFGKPVTPGRPGEKLLKGKSARVIVTMGMPGFAYRWFYGAHAVKSLERNILAFT
                     GFGPIRRTLIGMVEARDGSSRKKRLAEVRRLGARAL"
BASE COUNT          784 a         1896 c         1793 g          797 t
ORIGIN      
        1 gctgctgcag gcggcggtct cccggcagcg cgaaaagctg gccgacgcgg ccgcggtgca
       61 gttcacccgc aaccccgacg ggctggccaa cgcgctcaaa cgcatcgccg cctcgccgct
      121 gtcctcgtcc gtgcgcgcgg tgcggcgcga ggaaatcggc cacatgctgt tcgccagcgg
      181 gcggcgcagc ctggcgggac tgatggcgac gcacccgccg gtcgaggagc gcatccgcgc
      241 gctggatccc aactggcggc cgggtgaccc cccgccgccg ctgccgacgc cggtcgagcc
      301 ggccgagacc gccgccgagg cggcccgcaa ggagcgtatc cggcaggggg cgttgcccgg
      361 cctgccgggc ggcctgggcc tgccggggct cgacccggtc gccgcggcag cgatgatcgg
      421 cgtcgtggat ctcgccatgg ccgagcgact gctggcgatg gtgccggagg cgatccacca
      481 ggcggtgctc gagcccgaag gcgccgccgt cgtcgcgctg gccgtgctgc tggacagtgg
      541 cgaacggctg cgcgcccacc agctcgccat gctcgaggca cgcctgggca gcaccgtggc
      601 ggaccgcatc gcgcaggccg cgggccggct cgcagacgtc tcgccgacgc tgcgtctcgc
      661 cctgctggac ctcgcctccc ctgcgctgcg gcacctgccg gaggcgcgcc gcgaggacct
      721 gctgcacctg gcgcagcgcc tggtcgagat cgacggcgtg gtgacgcccc aggaggccgc
      781 gctggccgcg gcgctggaat cgcggctgtc acccgaggcc gacaggcaac ctgatcccga
      841 gggcgccctg gcgatgctgg ccctgctcgc gcgcgccggc cacgaagatg atgccgaggc
      901 ggaacaggcg tggcgagacg gtgccgctcg gctcgcggcc gccggcttcc cgctgccgtc
      961 cccgctgccg gaattcccgg ccacggaggg ctctgccggc gctgaatccg ccgcgcatgc
     1021 gccccggctg gccgccatgg cggagcgtca tcgacaggcg ctgctggctg cgctggcgga
     1081 agtggccgcc cacgacggcg tcattcgccc ggccgaactg cagctgctgc gcgccgcggc
     1141 cgccgccctc gactgcccga tgccgccgct gaacgtaccg ccctccggct agcgctccga
     1201 ctcgatcctg tagagcccgc cgtcggcctc gtcgctgagc acgtagatgt gcccgtccgg
     1261 cccttcgcgc acgtcgcgga tccggccgac cgtctgcagc agcagctcct cctcgtgcac
     1321 cacctcgccg tcctcgatca ccagccgccg gatgcgctgc gcgcgcagtc cgccggcgag
     1381 caggttgcct tgccagctcg ggaaacggtc cttggtcacc agcgccagcc cggacggcgc
     1441 caacgtcggc aggaactcgt acaccgggtc cacgtagccc gcccggctgc gcgcctcgcc
     1501 atactgctcc tcgctgcggt actggcgtgc cttgctcacc accggccagc cgtaattctt
     1561 gccggcctgc acccggttca gctcgtcgcc gccgcgctcg ccgtgctcgg tggcccagat
     1621 cgcaccgccg gcggcatcga ccaccatgcc ctgtatgttg cggtggccgg tcgagaagat
     1681 ctcggcatgc gcgtcgtcgc gaccgacgaa gggattgtca ttcggcacgc tgccgtcggc
     1741 gttcaggcgc agcaccttgc cggcgtggtc gcgcaggtcc tgggcgcgcg gcggcgacgc
     1801 gccgcgatcg ccgatgctca tcagcaaggt gccgtcgggc agccacgcga tgcgggaacc
     1861 gtaatggcgg ccgggctcgg accggcggtc ctgcacgaac agttcctcga agccgaccag
     1921 cgcgtcaccc tcgagccggc cacgccccag cgcggtggtc gtgccggcgg catcgccctt
     1981 cgagtaggtg atatagaccc agccgttggc tgtgaaatcc ggatgcagga cgacgtcgag
     2041 cagtccgccc tgtcgatgcg cgtggatctc cggcaggccg gcgacgcgcg tcttcacgcc
     2101 gtcggccaag aggttcaggc ggccggaccg ctcggagacc aggaagcggc cgtcgggcag
     2161 cacggcgacg gcccagggat gctcgaggcc gtcggcgatg cgcaccaccc ggatgggctc
     2221 atattcggtg cggaggcctt cggccacctt ctccgcggcc gcgtgcccgg tgggggcgac
     2281 cagccacgcc gccagcaggc tgggcatgac cattccatgg gccagttttt tcacagctct
     2341 cctccttact acagtccctc tcccagttcc tgcggcggcc cgccaccatg tggctgcgaa
     2401 ccccgcagcg gcagcgccac gcgcgccatc gccgcgtcga cctcttcctg gctgtcgcat
     2461 gccagcatcc ttcgtaccgt gcgttcatcc agatgcgcgg caaacatcgc gaagaaatcg
     2521 aacatgtact tgcgcagcac cacgtcgcga cggaaaccga tccaggtggt gcagcgcgga
     2581 aacagcccgg aggcgtcaag cgccacgagg ccgtcccgtg cgtcggcgct ccaggccatg
     2641 ctcgccacga tgccgatacc cagcccgcgc gccacgtaag tcttgatgac gtccgcatcg
     2701 cgcgcggtga acgccacgtt gggctccagt ccgcgcgccg cgaaagcgcg cttcaggctc
     2761 gagccgccgt caaagttgaa tacataagtg accagcgggt gcgccgcgag ttcctccagc
     2821 gtcggcgggc gccgcagcga cgccagcgcg tggccctccg gcacgagcac gatccggtcc
     2881 cagcgaaagc atggcagcac caccagttcc ggaaacctgt ggcgcgactc ggaggcgatg
     2941 acgaaatcca cgcgccgctg ttccgccatc tcggcgagct gctccgaggt gccctgatgg
     3001 aggtccaggg tcacttccgg atactgcgcc cggaagcagg cgatgacgtc cggcagcacg
     3061 tagcgcgcct gggtgtgagt ggtgccgatg gacagccggc ccgacacctc tgaatagatg
     3121 ttgggcgaga tcccccggat ggcctccacc tcgcgcagga tgcgcaccgc tcgctgcgcc
     3181 acctcctcgc cctccggggt caggctgacc aggctgcggc ccttgcgcac gaacaggctc
     3241 aggccgagct cctgttccag cagcttgagc tgcttgctga cgcccggctg cgaggtggcc
     3301 aggcgctcgg ccgcctgggt catattgagc ccgttgtcga tcaccgcccg caggtaacgg
     3361 agttgttgca acttcatcga tggtcctcgg ctggtcgaaa ccccgaaatt ttcaggcggc
     3421 catcatccgg gcctgctata agcaatgcac atagcgacag aaccaatcgt tatttccgtc
     3481 tggcgggcgc cagcttatat tcttttcagc ataagccatt gggaaagcca tgatccacga
     3541 cagcattctc ggcactatcg gcgacacgcc ggtcgtccgt ctcaaccgca tcggcccgga
     3601 ccacgtgacg atctacgtca agatcgagtc cttcaacccg atggcgtcgg tgaaggaccg
     3661 cctggccttc gccattatcg acgacgccga gaagcgcggt ctgctgaagc caggccagac
     3721 cgtcatcgag gcgacttctg gcaacaccgg cattgcgctc gccatggtct gcgccgcccg
     3781 cggctatccc ttcgtcgcca ccatggtgga aaccttctcc atcgagcggc gccgcgtcat
     3841 gaaggccctg ggcgcgaagg tcatcctgac gccggccgcc gagcgcggca gcggcatggt
     3901 acggcgcgcg gaggaactcg cgaagcagca cggctggttt cttgcccggc agttcgagaa
     3961 cgaggccaac ccggcgttcc accggcagac gaccggcccg gagattctca aggacttcgc
     4021 cgggcggcgg ctcgactact gggtcacggg ctggggcacc ggcggcacgc tgaccggcgc
     4081 cggtgaggtc atcaagctgg cccggccgga ggtgaagatc gtcgccaccg agccggaagg
     4141 cgcgtcgctg ctgggcggca aggaatggaa gccccacaag atccagggct ggacgccgga
     4201 tttcgtgccg caggtgctca atcgcgaggt ctacgacgag ctcgtgaccg tgacggacga
     4261 ggaatcgcgc gagtgggccc gtgcgctggc gacgcgcgag ggcattctcg tcggcctgtc
     4321 cgccggcggc acgctggccg cggcgctcaa ggtggcggag cgcgccgcgc cgggctcggt
     4381 gttgctggcg atgctgcccg ataccggcga gcgttacctg agcactttcc tcttcgaggg
     4441 catcgacgag agctccgacg acgcctggct ggctgaacag ggctgaccgc ccgccgcgcc
     4501 gacttcggca atccggcatg acgccggacc tgccgctctg gctaggatag ggccctcatc
     4561 caggcgggag cgcgcaacgt ggccaggcga atcgtcatcg tgcaggggca ccccgacccg
     4621 ggcggcggtc gctacggcca tgccctggcc gaggcctatc gcgagggggc ggccggcgcg
     4681 ggacatgaag tcgaggtgat ccccgtcgcg gagctcgact ttccgctgtt gcgctccaag
     4741 gcggacttcg atggcggcac cccgccggcc gccatcctgc gctgccagca gttgatcctg
     4801 caggccgatc acctggtcat catccatccg ctctggctgg gttccatgcc ggcgctgctg
     4861 aagggctttc tcgaacaggt cttccggccc gggttcgcct tcggcaagcc cgtgacgccc
     4921 ggccggccgg gcgagaagct gctcaagggc aagagcgccc gcgtgatcgt gaccatgggc
     4981 atgccgggct ttgcctaccg ctggttttac ggcgcccatg ccgtcaagag cctggaacgg
     5041 aacattctcg ccttcaccgg cttcgggccg attcgccgga cgctcatcgg catggtcgag
     5101 gcgcgcgacg ggtccagccg caagaaacgc ctggccgagg tccgccggct cggcgcccgg
     5161 gcactgtagc gtccgcccgg cgacgaccgt ggccgacagg tattttcgtc gtaccggatt
     5221 ttcccggaga atacgggtcc gggacgctgc cgcgaggcgg cgtcgacatc
//
LOCUS       DAOMPG010000171         5266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6613_length_5266_cov_4.353675, whole genome shotgun sequence.
ACCESSION   DAOMPG010000171 DAOMPG010000000
VERSION     DAOMPG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6613_length_5266_cov_4.353675"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLS34_09395"
     CDS             <1..458
                     /locus_tag="PLS34_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP87721.1"
                     /translation="GPMMSIKTVNALSHYTDWTIGHVHSGALGWVAMISIGCVYMLVP
                     PLFGKKQMHSIKAIDVHFWVSTIGVVLYIAAMWMAGVMQGLMWRAVNEDGTLTYTFIE
                     SIARTYPFYAVRLLGGVLFLGGMLIMCWNTWRTIRGAKAAEVPVMAPAH"
     gene            471..1079
                     /gene="ccoO"
                     /locus_tag="PLS34_09400"
     CDS             471..1079
                     /gene="ccoO"
                     /locus_tag="PLS34_09400"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRP87722.1"
                     /translation="MKHELVERKVGLMAVLIVLVISVGGLVEIVPLIFDSQVRDPAPG
                     IEPHLPLVLAGRDIFIREGCYGCHSQMIRPFRAETERYGHYSLAGESVYDRPFQFGSK
                     RTGPDLARVGGLYSDEWHRLHLIDPRMLVPESNMPGYPWLEKRGLDPRRIERRMLALR
                     RLGHPYSDEDIAGVGAALADKTEMDAMITYLQSLGTNLGRGR"
     gene            1082..1261
                     /locus_tag="PLS34_09405"
     CDS             1082..1261
                     /locus_tag="PLS34_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011496290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRP87723.1"
                     /translation="MSGDDWHIVWTLLLMVAFIGIVFWAWSSRRHKDFKEAANLPLEE
                     DERGPKSTLENGESR"
     gene            1258..2154
                     /gene="ccoP"
                     /locus_tag="PLS34_09410"
     CDS             1258..2154
                     /gene="ccoP"
                     /locus_tag="PLS34_09410"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRP87724.1"
                     /translation="MSSFWSGWIILLTVANIVGCLWLLWMTSRTSPGEKASDTTGHVW
                     DGDLQEYNNPLPRWWLWLFYLTVVFSVIYLVLYPGLGNFQGVLGWTQTGQYEEEVARI
                     DERQQEFFARFDNLEIVELARNSDAMAAAGNIFGNRCAQCHGSDGRGAPGFPNLTDDA
                     WLWGGDETAILASIQNGRSGMMPPMGDALGGERAVAQMVEYVRSLAGLDHDAALAAAA
                     QPMWAVCGACHGMDGTGMTALGAPNLTDNNWLYGSDRRTIAETITRGRQNNMPAQLPI
                     LGEQQSRLMAAYVLRLSGQAGR"
     gene            2165..2572
                     /locus_tag="PLS34_09415"
     CDS             2165..2572
                     /locus_tag="PLS34_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87725.1"
                     /translation="MARSPQPQHDESGDRPRIWRRRQRDVAYVAWASFLMACAASAVF
                     FAMVDPEVLSGTTTPGWEISRQAGYAIGFFGFWMLTAATALFAIWLVRTELPGPPGGT
                     RRPGDGQRSGGRRGGPLRRRLRPRAHQGREERQ"
     gene            2569..3981
                     /gene="ccoG"
                     /locus_tag="PLS34_09420"
     CDS             2569..3981
                     /gene="ccoG"
                     /locus_tag="PLS34_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRP87726.1"
                     /translation="MNAQPQGGAGGAAPESFYVAHEKIYPREVKGRYATLRNLAVWVL
                     LGIYYLLPWIPWGDRQAVLFDLPARKFYIFGLTFWPQDFFFMAWLLIIAALTLFFVTA
                     IAGRLWCGYACPQTVWTEAFLWMERLAEGNHSQRRKLDKGPWTREKVLRKGAKQVMWI
                     GFALWTGFTFVAYFTPATELGAKLLTFSAGPWETFWVLFYGFATYGNAGLLREQVCKY
                     MCPYARFQSAMFDKDTMIISYDARRGEPRGGRKRSIDPKAAGLGDCIDCNMCVQVCPT
                     GIDIREGLQIECIACAACVDVCNDVMDKMAYPRGLIRYSTHHAMEERQPARVLRPRVM
                     IYGALLLTLLVGFGVAVANRSTLIVDVIRDRNMLYRELPGGLVENVYTVTFVNKTEAE
                     RSYRVTARGLEGLEVKVDAPLPLAAAPGEVRRVPLRLQVPVRAVPAGGAEVTLVVEAL
                     GEAPATVERATRFIGPADPP"
     gene            3978..4496
                     /locus_tag="PLS34_09425"
     CDS             3978..4496
                     /locus_tag="PLS34_09425"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRP87727.1"
                     /translation="MNTPTRTALGEGRHPEPWYRQFWPWFLIALPASVVIASMVTIWL
                     AVSRPNPMVVDDYSRIARSIEQRRERDLAAAALGVTAEIRFIADAGLVVVRLAPESVE
                     PASLQLRLSHPLVEERDQVVQLTRSPDGWSGSVETPEGRWYVQIYPGDLGWRLSGELD
                     GERELALAPPAP"
     gene            4503..>5266
                     /locus_tag="PLS34_09430"
     CDS             4503..>5266
                     /locus_tag="PLS34_09430"
                     /inference="COORDINATES: protein motif:HMM:NF023578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRP87728.1"
                     /translation="MTESACFHCGEPLNGTEPWFVEIEGRSHRVCCPGCKAVAELIGA
                     SGLADFYRFRTLPAGRPADITAAEAPDLPWRPFDRPEMLEAVSAPAGEGLRSCTMLVE
                     GVRCAACGWLIERSLEQVPGVREIRVNPASSRARLVWDPARLPLSGALGVLSRLGYRP
                     HPGGAGAAEAAQRERRTALRRIGVAGIGMMQVMMYAVALWVGVFQDMDPGLEQLFRWV
                     SMLVATPVALYSALPFYAGAWRDIRSGRPGMDVPVSL"
BASE COUNT          802 a         1745 c         1839 g          880 t
ORIGIN      
        1 aggggccgat gatgtccatc aagacggtca acgcgctgtc ccactacacc gactggacca
       61 ttggccacgt gcactccggc gcgctcggct gggtggcgat gatctccatc ggctgcgtct
      121 acatgctggt gccgccgctg ttcggcaaga aacagatgca cagcatcaag gccatcgacg
      181 tccatttctg ggtgtcgacc atcggcgtag tgctgtacat cgcggcaatg tggatggccg
      241 gcgtcatgca gggcctgatg tggcgggcgg tcaacgagga cggcacgctc acctacacct
      301 tcatcgagag catcgcccgc acctatccgt tctacgcggt gcgactgctg ggcggcgtcc
      361 tgttcctcgg cggcatgctg atcatgtgct ggaacacctg gcgcacgatc cgcggcgcaa
      421 aggctgcgga agttcccgtc atggctccgg cgcactaggg ggaagctggc atgaagcacg
      481 aactcgtgga aagaaaagtc ggcctcatgg cggtgctgat cgtcctggtg atcagcgtcg
      541 gcgggctggt ggagatcgtt ccgctgatct tcgactcgca ggtcagggac cccgcgccgg
      601 gaatcgagcc gcacctgccg ctggtgctgg cggggcgcga catcttcatc cgcgaaggct
      661 gttacggctg ccactcgcag atgatccggc cgttccgcgc cgagaccgag cgttacgggc
      721 actattcgct cgccggcgag tcggtttatg accggccctt ccagttcggc tccaagcgca
      781 ccgggcccga cctggcgcgc gtcggtggcc tgtacagcga cgagtggcac cgcctgcacc
      841 tgatcgaccc ccgcatgctg gtgcccgagt cgaacatgcc gggctacccc tggctcgaga
      901 agcgcggcct cgacccgcgt cgcatcgagc ggcggatgct ggccttgcgc aggctcggcc
      961 acccctactc cgacgaagac atcgccggcg tgggggcggc gctggcggac aagaccgaga
     1021 tggacgccat gatcacttac ctgcagagcc tcggcaccaa cctgggaagg ggccgctgaa
     1081 ggtgagcgga gacgactggc atatcgtctg gacgctgctc ctgatggtgg cattcatcgg
     1141 gatcgtcttc tgggcgtgga gcagccgccg gcacaaggac ttcaaagagg ccgcgaacct
     1201 cccgctggag gaggacgagc gcggacccaa gagcacgctt gagaacgggg aatcgcgatg
     1261 agcagtttct ggagcggatg gatcattctg ctgaccgtgg ccaacatcgt cggctgtctg
     1321 tggctgctgt ggatgacgtc gcggaccagt cccggcgaga aggcttcgga cacgaccggc
     1381 cacgtgtggg acggcgacct gcaggagtac aacaacccgc tgccgcgctg gtggctgtgg
     1441 ctgttctacc tgacggtggt gttctcggtg atctacctgg tgctgtatcc cggcctgggc
     1501 aatttccagg gcgtgctcgg ctggacccag acgggccagt acgaggaaga agtggcccgg
     1561 atcgacgaac ggcagcagga attcttcgca cgcttcgaca atctcgagat cgttgaactg
     1621 gcccgcaaca gcgatgccat ggcggcagcc ggcaacatct tcggcaaccg ttgtgcccag
     1681 tgccacggct ccgacggccg cggtgcgccc ggcttcccga acctgaccga cgatgcgtgg
     1741 ctgtggggcg gcgacgaaac cgcaattctc gccagcatcc agaacggccg ctcgggcatg
     1801 atgccgccga tgggcgatgc gctcggcggc gaacgcgccg tggcgcagat ggtcgagtac
     1861 gtgcgcagcc tcgccggcct ggaccacgac gccgcgctgg cggccgcggc gcagccgatg
     1921 tgggcggtgt gcggcgcctg ccacggcatg gacggcacgg gcatgaccgc gctcggcgcg
     1981 ccgaacctga ccgacaacaa ctggctgtac gggtccgacc ggcgcaccat cgccgagacc
     2041 atcaccaggg gccgccagaa caacatgccg gcacaattgc cgatactcgg cgagcagcag
     2101 tcccggctga tggcggccta cgtgctgcgc ctctccggac aggccggccg gtaagaggta
     2161 cggcatggcg cgcagcccgc agccgcaaca cgacgagagc ggcgaccgcc cccggatctg
     2221 gcgccgccgt caacgcgacg tcgcctacgt ggcatgggcg tctttcctga tggcctgcgc
     2281 ggcatccgcg gtgtttttcg ccatggtgga tcccgaggtg ctgtccggaa cgaccacgcc
     2341 gggctgggaa atcagccggc aggccggcta cgcgatcggc tttttcggct tctggatgct
     2401 gactgcggcg actgccttgt tcgccatctg gctggtgcgc acggaactgc cggggccgcc
     2461 cggtggaacg cgccggcccg gcgacggtca gagaagcggc ggcaggcgcg gcggcccgtt
     2521 gcgcaggcgg ctgcgccccc gcgcgcacca gggtcgagag gaacggcaat gaatgcccag
     2581 ccccagggcg gcgccggcgg cgccgcgcct gagtctttct acgtcgcgca cgagaagatc
     2641 tacccgcggg aggtcaaggg tcgctatgcc acgctgcgca acctcgcggt gtgggtcctg
     2701 ctgggcatct attacctgct gccgtggatt ccctggggtg accgccaggc ggtgctgttc
     2761 gacctgccgg cccgcaagtt ctacatcttc gggctgactt tctggccgca ggatttcttc
     2821 ttcatggcgt ggctgctgat catcgccgca ctcacgctgt ttttcgtcac ggcgatcgcc
     2881 ggccggctgt ggtgcggcta cgcctgcccg cagacggtgt ggaccgaggc cttcctgtgg
     2941 atggagcgcc tggcggaggg caatcacagc cagcgtcgca agctggacaa ggggccgtgg
     3001 acgcgcgaaa aggtgctgcg caagggcgcc aagcaggtca tgtggatcgg cttcgcgctg
     3061 tggacgggtt tcaccttcgt cgcctatttc acgccggcga ccgagctcgg cgccaagctc
     3121 ctgacttttt ccgccgggcc gtgggaaacc ttctgggtgc tgttctacgg tttcgccacc
     3181 tacggcaatg ccgggctgct ccgcgaacag gtctgcaagt acatgtgtcc ttacgcgcgc
     3241 ttccagagcg cgatgttcga caaggacacc atgatcatct cctacgatgc ccgccgcggc
     3301 gaaccgcgcg gcggccgcaa gcgctcgatc gacccgaaag cggccgggct cggcgactgc
     3361 atcgactgca atatgtgcgt gcaggtctgt ccgaccggca tcgacatccg cgaggggctg
     3421 cagatcgagt gcattgcctg tgccgcctgc gtggacgtct gcaacgacgt gatggacaag
     3481 atggcgtacc cgcgcggcct gatccgctac tccacccacc acgcaatgga agagcgccag
     3541 ccggcgcgcg tgctgcggcc gcgggtgatg atctacggcg cgctgctgct gaccctgctg
     3601 gtcggcttcg gcgtcgccgt cgccaaccgc agcacgctga tcgtcgacgt cattcgcgat
     3661 cggaacatgc tctaccgcga actgccgggt ggactggtcg agaatgtcta taccgtcacc
     3721 ttcgtcaaca agaccgaggc cgagcgcagc taccgcgtaa ccgcgcgcgg gctagaggga
     3781 ctggaggtga aagtcgacgc accgctgccg ctcgcggccg cgccgggcga agtgcggcgc
     3841 gtgccgttgc gcctgcaggt gcccgtgcgc gcggtgccgg ccggcggcgc cgaagtgacg
     3901 ctggtcgtgg aagcgctcgg cgaggcgccc gcgaccgtcg agcgcgccac acgcttcatc
     3961 gggccggcgg acccgccgtg aacacaccga cccgcacggc gcttggagag ggccgccatc
     4021 cggagccctg gtaccggcag ttctggccgt ggttcctgat cgccctgccg gcttcggtgg
     4081 tgatcgccag catggtcacc atctggctgg cggtgagccg gccgaacccg atggtggtgg
     4141 atgattattc ccgcatcgcc cgctccatcg agcaacgccg cgagcgcgac ctcgccgccg
     4201 ccgcactcgg cgtcaccgcc gagatccgtt tcattgccga tgccgggctg gtggtggtcc
     4261 gcctggcgcc ggaatcagtc gagccggcgt cgctgcaatt gcgtttgtcg cacccgctgg
     4321 tggaggagcg cgaccaggtg gtgcagctca cccgctctcc cgatggctgg agcggcagtg
     4381 ttgagacgcc cgagggacgc tggtatgtgc agatctaccc gggggacctg ggctggcgcc
     4441 tgtccggcga gctcgacggc gagcgcgaac tggcgctggc gccgccggcg ccctgagcca
     4501 tcgtgaccga gagcgcctgc ttccactgcg gcgagccgct gaacggcacg gagccttggt
     4561 tcgtcgagat cgagggacgg agccaccggg tctgctgtcc cggctgcaag gcggtggccg
     4621 agttgatcgg cgccagcggc ctggccgatt tctatcgctt tcgcacgctg cccgccgggc
     4681 gacccgccga cattaccgcc gcagaggccc cggacttgcc ctggcggccg tttgaccggc
     4741 cggagatgct cgaggcggtg agcgcgcccg ccggcgaagg cctgcgcagt tgcaccatgc
     4801 tggtcgaggg cgtgcgctgc gcggcgtgcg gctggctgat cgagcgcagc ctcgaacagg
     4861 tgcccggcgt gcgcgagatc cgggtcaacc cggcctcttc gcgcgcccgc ctggtgtggg
     4921 acccggcccg tttgccgctc agcggcgccc tcggcgtgct ctcgcgactg ggttaccggc
     4981 cccatccggg cggcgccggc gccgccgagg ccgcgcaacg cgagcggcgc acggcactgc
     5041 gccggatcgg cgtggccggc atcggcatga tgcaggtgat gatgtacgcg gtggcgctct
     5101 gggtcggcgt gttccaggac atggatcccg gcctggagca gctgttccgc tgggtcagca
     5161 tgctggtggc gaccccggtt gcgctttatt cggccttgcc gttctacgcc ggcgcctggc
     5221 gtgatatccg ttccggccgg ccgggcatgg acgtgccggt atccct
//
LOCUS       DAOMPG010000172         5224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6670_length_5224_cov_3.591604, whole genome shotgun sequence.
ACCESSION   DAOMPG010000172 DAOMPG010000000
VERSION     DAOMPG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6670_length_5224_cov_3.591604"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLS34_09435"
     CDS             <1..201
                     /locus_tag="PLS34_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87729.1"
                     /translation="TPGITLQSVSFRAGVLDLRLLAPSVDSLEQIRQSVGRGGRFTAT
                     IQQANQRPDGVDGRIQLTGDGA"
     gene            240..686
                     /locus_tag="PLS34_09440"
     CDS             240..686
                     /locus_tag="PLS34_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HRP87730.1"
                     /translation="MVSVGGVVLLLAVIWGLLINPVYSASAAAERRIDTKRETLAFLR
                     SAAAELAGATDVPAGRPDLAGQSLVVIIDRSARQAGLGAALTSNQPIGEDGIRVRLES
                     ASFEAVARWLAAINQGSGLAIETASFDRAPESGRVNANLTLRQGLP"
     gene            683..1429
                     /locus_tag="PLS34_09445"
     CDS             683..1429
                     /locus_tag="PLS34_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HRP87731.1"
                     /translation="MSLVERRWMLVAAGMLALLAGLVANFPARLALAWFAPPEVRAWG
                     VEGSIWRGRAAELVVQQSAMGALSWQSRPARLLAMQPAWDLELRRPDGYVRGRAAFAL
                     LADRQVINDLEASLALETLPPAIVPKGLSGQVRASLQRLELSGGWPASVAGRAAVAEL
                     KLPGVIMPLGPFSFHFPDQPGTPVGEIVSTGGPLAVDGRIELPAHGQWNFSAELAPGE
                     NPPKELIDGLAFVGEDLGGGRRRLVMSSHP"
     gene            complement(1464..4853)
                     /locus_tag="PLS34_09450"
     CDS             complement(1464..4853)
                     /locus_tag="PLS34_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRP87732.1"
                     /translation="MTERRPEDQAVREAALDPARSFIVQAPAGSGKTELLTRRFLRLL
                     AVVERPEEIVAITFTRKAAGEMRSRILDALQLGESLQAPAEAHRREAWSLARDVLARD
                     RAQHWGLLDNPRRLRIETIDALNAWLARQLPMSSRLGAAPVVTDNAEPVYQQTARDIL
                     LAAGGDEAHAPHAAALLVHLGGRFGQAQALLVAMLKARDHWLGKIVPRKGMQPSMLRK
                     TLNQALHNFVEDELRRVHAELWPEMLDELAELAAGAAARAPQIDWLEPFAGATSFPAP
                     SAEALPRWRAVAELLLTKEGQWRKTLNKHQGFPPDAKSEKQRMRELLQALAEGADPAG
                     LQRVRELPAPAYDAERWAILEHLLALLPVCAAALDVDFAGRGESDYVGIAQAALAALG
                     DDRPTDLALALDCRISHLLVDEFQDTSQAQVELLARLTAGWVPGDGRTLFCVGDPMQS
                     IYRFREADVGRFLRAQRTGIGQVTLQRLRLGVNFRSQASLVDWVSTSFPDIFPRREDL
                     SLGAVPFAPSVAWHPALPGPAVNCHAAPPGDSRTEAARVAGIIAGIRQAAPAASIAVL
                     VRSRSHLAHITPALKAAAIPFQAVEIEPLAEVPAIRDLEALTRALCHRADRTAWLAIL
                     RAPWCGLPLAELLAVAGGRHDEIWARLQDPEVLAGLSEDGRARVARLVVAIAPALEER
                     GRRPLRRVVEGAWLALGGPATLERESELADARTFLEYLDGRAPHGDLDDPPGLTALLG
                     DLYAAPEAITGTAVQLLTIHKAKGLEFDHVILPGLDRSTGRNEKRLLRWLEQVREEGT
                     ELILAPVERFGDEQDPLHRALRQLDDRRAQLELDRLLYVAVTRARERLHLVARLRPDD
                     PDSGQPPRPRSGTLLERLWPALGDAFLAAREPVGNVPGEGAARPPAKLRRYTADWSPP
                     VPLAAVHWTAAEAEPAAPGAVVEYDWSGREARAVGVAVHRLLQVISTEGPDAWPEARL
                     RAATPLLRALLSEAGLGRAELDAALARSSAVLARTLADARGRWLLDPGHADSSSERRI
                     SGRLDGRVVQGIVDRSFLDESGVLWIIDYKTGRHEGAELDAFLDREQERYRAQLERYA
                     RMLAARHAGPIRMGLYFPQLSGWREWGAGDPPPDRETPAGE"
     gene            complement(4846..>5224)
                     /locus_tag="PLS34_09455"
     CDS             complement(4846..>5224)
                     /locus_tag="PLS34_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRP87733.1"
                     /translation="PGDWQGERPAEPQMPLYAIAFQHELAGLAYASLRPGAVGLAGLA
                     RSDQVFGAALPKLKPPAADEWRALIDAWQQVLEALAHAFAAGYARVDPLEPRGEGGTC
                     AWCHLATLCRRDQLLREGALGDD"
BASE COUNT          727 a         1903 c         1849 g          745 t
ORIGIN      
        1 acgcccggga tcacgctcca gtcggtcagc ttccgggccg gggtgcttga cctgcggctg
       61 ctggcgccga gtgtcgactc actggagcag atccggcagt cggtcggccg cggcggacgt
      121 ttcaccgcga ccatccagca ggccaaccag cgaccggacg gcgtcgacgg gcgtatccag
      181 ctgaccggag acggggcatg aaggactggt acctcgggct cgagccgcgc gagcgcctga
      241 tggtttcggt cggcggcgtg gtgctgttgc tcgcggtgat ctggggtctg ctcatcaacc
      301 ccgtgtacag cgcgtcggcc gcggccgaac gccgcatcga cacgaaacgc gaaaccctgg
      361 cgttcctgcg ctcggccgcg gccgaactgg cgggcgcaac ggatgtcccg gcaggtcgcc
      421 ccgatcttgc cggtcagtcc ctggtggtga tcatcgatcg cagcgcgcgc caggcgggac
      481 tcggcgccgc cctgaccagc aaccagccga tcggcgagga cggcatccgg gtgcgcctgg
      541 aaagcgcgtc gttcgaggcg gtggcgcgtt ggctggcggc aatcaaccag gggtccggac
      601 ttgccatcga gacggccagt ttcgaccggg caccggaatc cggccgggtc aatgcgaacc
      661 tgacgctgcg ccaaggcctg ccatgagcct ggtcgagcga cgctggatgc tcgtcgcggc
      721 gggcatgctc gccctgctgg cagggctggt ggcgaatttc ccggcgcggc tcgcgctggc
      781 ctggttcgcc ccgccggagg tgcgcgcatg gggcgtggaa ggcagcatct ggcgcgggcg
      841 cgcggccgag ctggtggtcc agcagagcgc tatgggcgcg ctgtcctggc agtcccgccc
      901 ggcccgcctg ctcgccatgc agcccgcctg ggacctcgag ctgcggcgtc ccgacggcta
      961 cgtgcggggg cgcgcggctt tcgccctgct cgccgaccgg caggtcatca acgacctgga
     1021 ggcctccctg gccctcgaga cgctgccgcc ggcgatcgtg ccgaagggcc tgtcgggcca
     1081 ggtcagggcg tcgctgcaac gcctcgaact gagcggcggc tggccggcca gtgtcgccgg
     1141 ccgggctgcg gtggcggagc tcaagctgcc gggagtgatc atgccgctcg gcccgttcag
     1201 cttccatttt cccgaccagc cgggcacacc ggtcggcgag atcgtgtcca ccggcggccc
     1261 gctcgcggtg gacggccgga tcgagttgcc ggcacacggc caatggaatt tcagcgccga
     1321 gctggccccg ggcgagaatc ctcccaagga gctcatcgac gggctggcct tcgtcggtga
     1381 agacctgggc ggcggtcgcc gccggctggt catgtcgagc cacccctgat cgagcgaccc
     1441 cggtcgagcc acgccgcgcg gcgtcactcc cctgccgggg tttcccggtc cggcggcggg
     1501 tcgcctgcgc cccattcgcg ccagccggaa agctgcggga aatagagtcc catcctgatc
     1561 ggtccggcgt gccgcgcggc gagcatccgg gcgtagcgtt ccagctgggc gcggtagcgt
     1621 tcctgctcgc ggtcgaggaa ggcgtcgagc tccgcaccct cgtggcggcc ggttttgtag
     1681 tcgatgatcc acagtacgcc cgactcgtcc aggaaactgc ggtccacgat cccctgcacc
     1741 acccggccgt ctagccggcc gctgatgcgg cgttcgctcg agctgtcggc atgcccggga
     1801 tcgagcagcc agcgaccgcg cgcatcggcc agcgtgcgcg ccagcaccgc gctgctgcgc
     1861 gccagcgctg cgtcgagttc cgcccggccg agaccggcct cgctcagcaa ggcacgcaac
     1921 aacggcgtcg cagcgcgcag ccgcgcttcg ggccaagcat ccggcccctc cgtcgaaatg
     1981 acctgcagca ggcgatgcac cgccacgccg accgcccggg cttcccggcc agaccagtcg
     2041 tactcgacca cggcgcccgg cgcagccggc tcggcttccg ccgcggtcca gtgcactgct
     2101 gccagcggca ccggcggcga ccagtcggca gtataccgcc gcagcttggc gggcggccgg
     2161 gcagcgccct cgccgggcac gttcccgacg ggctcgcgag cggcgagaaa ggcgtcgccg
     2221 agcgccggcc acaaccgctc gagcagagtg ccgcttcgcg gccgcggcgg ctggccgctg
     2281 tctggatcgt cgggccggag ccgggccacc aggtgcaggc gctcgcgcgc ccgcgtgacc
     2341 gccacgtaca acaggcggtc gagttccaac tgcgcccggc gatcgtccag ctggcgcagc
     2401 gcgcgatgca gcggatcctg ttcgtcgcca aagcgctcga ccggcgccag gatcagttcc
     2461 gtgccctcct ccctgacctg ttccagccag cgcagcagac gcttctcgtt gcggccggtg
     2521 ctgcgatcca gcccgggcag gatgacgtga tcgaattcca ggcccttggc cttgtgaatg
     2581 gtgagcagct gcacggccgt gccggtgatc gcctcgggcg cggcataaag gtcgccgagc
     2641 agcgccgtca gtcccggcgg gtcgtccagg tcgccgtgcg gcgcgcgtcc gtcaaggtat
     2701 tcgaggaagg tgcgcgcgtc cgccagctcc gattcgcgct ccagggtggc cggcccgccg
     2761 agcgcgagcc aggcgccctc caccacccgc cggagcggac gccggccccg ttcctcgagt
     2821 gccggcgcaa tcgccacgac caggcgggcg acgcgcgccc gcccgtcctc gctcagtccg
     2881 gccagcacct ccggatcctg aagtcttgcc cagatttcgt cgtgccgccc gcccgccacc
     2941 gccagcaact cggccagcgg cagtccgcac cacggcgccc gcaggatcgc gagccatgca
     3001 gtgcggtccg cccgatggca cagcgcgcgg gtcagcgcct cgagatcccg gatcgccggc
     3061 acttcggcca ggggttcgat ctccacggcc tggaacggaa tggccgccgc cttgagggcc
     3121 ggcgtgatgt gcgccaggtg gctgcggctg cgtaccagca cggcgatgct ggccgcgggg
     3181 gctgcctgcc ggatgcccgc gatgatcccc gcgacccggg cagcttccgt acgcgaatct
     3241 ccgggcggcg ccgcatgaca gttgacggcc ggcccgggga gtgccggatg ccaggccacc
     3301 gatggcgcga agggcacggc gcccagcgac aggtcctccc gccggggaaa aatgtccggg
     3361 aagctggtgg aaacccagtc gaccagcgac gcctggctgc ggaagttcac tcccagccga
     3421 agccgctgca gggtgacctg gccgatgccg gttcgttgtg ccctgagaaa acggcccacg
     3481 tccgcctcgc ggaagcggta gatcgactgc atggggtcgc cgacgcagaa cagcgtgcgg
     3541 ccgtctcccg gcacccagcc tgcggtgagc cgcgccagca attccacctg cgcctgcgag
     3601 gtgtcctgga actcgtccac cagcaggtgg ctgatgcggc agtccagcgc cagggcgaga
     3661 tcggtaggcc ggtcgtcccc gagagcggcg agcgcagcct gggcgatccc gacataatcg
     3721 ctctcgccgc gcccggcaaa gtcgacgtcg agtgccgccg cgcagaccgg cagcaacgcc
     3781 agcaggtgct cgagtatcgc ccatcgctcc gcgtcgtagg ccggcgcggg cagctcccgg
     3841 acgcgttgca gtcccgccgg atccgcgccc tcggccagcg cctgcagcag ttcgcgcatc
     3901 cgttgcttct ctgacttcgc atcgggcggg aagccctggt gcttgttgag cgtcttgcgc
     3961 cactggccct ctttcgtgag cagcaactcg gccacggcac gccagcgcgg cagggcctcg
     4021 gcactcggtg ccgggaaaga tgtcgcgccc gcgaacggtt cgagccagtc gatttgcggc
     4081 gcgcgtgcgg cggcgccggc cgcgagctcc gccaactcgt cgagcatctc gggccacagc
     4141 tcggcgtgca cgcggcgcag ttcatcctcg acgaaattgt gcaacgcctg gttgagcgtc
     4201 ttgcgcagca tcgatggctg catgcccttg cgcggcacaa tcttccccag ccagtggtcg
     4261 cgcgccttga gcatggcgac cagaagcgcc tgtgcctgcc cgaatcgtcc acccaggtgc
     4321 accagcaggg cggccgcgtg cggcgcatga gcttcgtcgc cgccggccgc cagcaggatg
     4381 tcgcgggccg tctgttggta cacgggctca gcgttgtcgg tcaccaccgg cgccgcgccg
     4441 aggcgcgagg acatcggcag ttgccgggcg agccaggcgt tcagcgcgtc gatggtctcg
     4501 atccgcagcc ggcgcggatt gtccagcagg ccccagtgct gcgcacggtc gcgtgccagc
     4561 acgtcgcgcg ccagcgacca ggcctccctg cgatgtgcct cggccggcgc ttgcagggac
     4621 tcgccgagct gcagcgcatc gagaatgcgc gagcgcattt cgcccgcggc cttgcgcgtg
     4681 aacgtgatgg caacgatctc ctccgggcgc tcgaccaccg cgagcagccg caggaagcga
     4741 cgtgtcagca gttccgtctt gccggagccg gccggcgcct ggacgatgaa gctccgcgcc
     4801 gggtccagcg cggcctcgcg aacagcctgg tcttcgggcc ggcgctcagt catcaccgag
     4861 cgctccctcg cgcagcagtt ggtcccgccg gcacaacgtg gccaggtggc accaggcgca
     4921 ggtaccgccc tcgcccctgg gctcgagcgg atcgacgcgc gcatagcctg cagcgaaggc
     4981 gtgcgccagc gcttcgagta cctgctgcca ggcgtcgatc agggcgcgcc attcgtcggc
     5041 cgcggggggc ttcaactttg gcagcgccgc gccgaacacc tggtcggagc gcgccagccc
     5101 ggccagcccg accgcgccgg ggcgcagcga cgcgtacgcc agcccggcca actcgtgctg
     5161 gaaggcgatg gcgtaaagcg gcatctgcgg ctcggccggg cgctcgccct gccagtcacc
     5221 gggg
//
LOCUS       DAOMPG010000173         5219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6682_length_5219_cov_4.269558, whole genome shotgun sequence.
ACCESSION   DAOMPG010000173 DAOMPG010000000
VERSION     DAOMPG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6682_length_5219_cov_4.269558"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLS34_09460"
     CDS             <1..646
                     /locus_tag="PLS34_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP87734.1"
                     /translation="TSEATGRRLAALGVEGGRITVAPPGTEPAPRAAAAGEPPLVVSV
                     GSLIRRKRHDLLLAALARVRDRPWRARIVGSAELDPATAAELARQVEALGLAGRVVLA
                     GAVADVAAELAAADVFALASEYEGYGMAFAEALAHGLPVVACRAGAIADLVPEAAGGL
                     VAPGDAAAFAAALARLLDDPAARQAAADAAWAAGRALPDWRRTAELVDAALARA"
     gene            643..1668
                     /locus_tag="PLS34_09465"
     CDS             643..1668
                     /locus_tag="PLS34_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRP87735.1"
                     /translation="MSFTAEWLELRAPADAAARDPALRAAAAEHLGRDGVALDLGCGT
                     GALAAAVIERCPQSTVLGIDFAATYVDYARGRVQGRIANADGTSRPAAIIEHADVRRM
                     LLLQKAYAEGALALALYSARLLDEQLVGDEACAARASRLLDLLTPVVKAWPSEYGLAA
                     NDLAIQIHGGYGYTRDYDVEQLYRDNRLNPIHEGTTGIQALDLLGRKLLRDRGAALAE
                     LRALVSATVAAADADPAQARHAKQLDTAWKRVGEVVDTLLEADAAHVLDDATPFLWAF
                     GHAVVAWLWLDMARLCTGDTPFHRGKRAAARCFFEYELPRVDAWLDQVAARSDLLVST
                     APELFHS"
     gene            1680..2858
                     /gene="bktB"
                     /locus_tag="PLS34_09470"
     CDS             1680..2858
                     /gene="bktB"
                     /locus_tag="PLS34_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HRP87736.1"
                     /translation="MTDVFIVSAVRTAIGTFGGALKDTPPSQLGVATAREAIARAGIP
                     FEAIGHAVYGQVLPSEPRDAYMARIIAIESGLPVATPALTVNRLCGSGLQAIVSAAQS
                     LLLGDCDVALAGGAESMSRTPFYLATHRWGRKLGDATLVDGLNEGLKDPFNQVLMGIT
                     AENIAQRHGIDRARQDAIALESHRRATAAIARGRFDGQIVPVTVGRHDKETEFRSDEH
                     VRAQLSADDLAGLRPVFKPDGSVTAGNASGINDGAASLVLATGEAVERHGLKPLARLI
                     AYAHAGVEPLYMGMGPVPATRAALARAGLSVNDLDVIESNEAFAAQCGAVSDELGLDP
                     AKVNPNGSGIALGHPVGTTGAIITVKLVHELQRSGGRYGLATMCIGGGQGIAAIFERT
                     "
     gene            complement(3028..3582)
                     /locus_tag="PLS34_09475"
     CDS             complement(3028..3582)
                     /locus_tag="PLS34_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87737.1"
                     /translation="MTMTVLHCSQKLLKRLRQPPTPPEPPPAGNPLGPWHGDIDFIDR
                     QPFAVLMNAATGTSLVLPARAADLKQLHVMAAAQLAALLQACGIDGALAQAELDALAE
                     PFAYARNRDRSWTAAMNQRRFEVWSQFAHGDLPAFEVALRTLETPFSRKDLGRDFHFA
                     ADLLRQHLRPSAKIVALGSAPGLH"
     gene            complement(3579..3878)
                     /locus_tag="PLS34_09480"
     CDS             complement(3579..3878)
                     /locus_tag="PLS34_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRP87738.1"
                     /translation="MTRLAFHPEALDEFEAAARYYEQQQAGLGNRFVGAVELALDTIL
                     HAPLTWPALAQDVRRKLVRVFPYALLYSVEPDGVLVLAVMHCHQRPGYWRHRTGA"
     gene            complement(3875..4105)
                     /locus_tag="PLS34_09485"
     CDS             complement(3875..4105)
                     /locus_tag="PLS34_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRP87739.1"
                     /translation="MNVSIEQIAEAALALPSDARALLADRLVESLDPLTDPEIRDAWA
                     AESLHRLDEVRSGKVKAVPADEVFARVRSGLK"
     gene            4188..>5219
                     /locus_tag="PLS34_09490"
     CDS             4188..>5219
                     /locus_tag="PLS34_09490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRP87740.1"
                     /translation="MPELNWLGDHQARRSARRVAYRLLETVEQVGAADDGQRDNLLIQ
                     GDNLEALKSLLPLYAGRVKCIFIDPPYNTKSAFEHYDDNLEHSQWLSMIYPRLVLLRE
                     LLAEDGSIWVTIDDNEAHYLKVLMDEVFGRGNFVTSCTWQQRTTRENRKVFSEDCESI
                     LLYCRDPELFKAKRNDLDLTEAVKARYKNPDDDPRGPWQSVSLNAQGGHGTPSQFYEF
                     IAPSGKVHRLPAGRCWLYTWPVMQEKIADSRVWFGAEGNNAPREKKFLSEAKAGLTPN
                     TLWLAADVGTNDEAKKETVTLFGEDVFETPKPERLIQRILHIATNANDLVLDSFLGSG
                     TTAAVAHKMG"
BASE COUNT          835 a         1800 c         1852 g          732 t
ORIGIN      
        1 caccagcgag gcgacggggc ggcggctggc ggcgctcggg gtcgagggcg ggcggatcac
       61 cgtcgcgccg cccggcacgg agccggcgcc gcgcgccgcg gcggcggggg agccgccgct
      121 cgtcgtcagc gtgggctcgc tcatccggcg caagcggcac gacctgctgc tcgcggcgct
      181 ggcgcgggtc cgcgaccggc cgtggcgggc gcggatcgtc gggtcggcgg agctcgatcc
      241 ggccacggcg gcggagctgg cgcggcaggt cgaggcgctc ggcctcgccg gccgcgtggt
      301 gctggccggg gcggtggccg acgtggcggc ggagctggcg gcggcggacg tcttcgcgct
      361 ggcgagcgag tacgagggct acggcatggc tttcgccgag gcgctggcgc acgggctgcc
      421 ggtggtcgcc tgccgggcag gggcgatcgc cgacctggtg ccggaggcgg ccggcggcct
      481 cgtcgccccg ggggacgcgg cggccttcgc ggcggcgctg gcgcggctcc tcgacgatcc
      541 ggctgcgcgg caggcggccg cggatgccgc ctgggcggcg ggccgggcgc tgccggactg
      601 gcggcggacg gccgagctgg tcgacgccgc cctggcccgg gcgtgagctt cacggcggaa
      661 tggctggagc tgcgcgcgcc cgccgacgcc gcggcgcgcg acccggcgct ccgggccgcg
      721 gcggcggaac atctcgggcg ggacggggtc gcgctcgatc tcggctgcgg cacaggcgcg
      781 cttgcggccg cagtcatcga gcgctgtccg caatcgacag tgctcggcat cgactttgcg
      841 gcgacgtacg tcgactacgc gcgcggccgc gtgcagggac gcatcgccaa tgcggatggc
      901 acgagccggc cggccgcgat catcgagcat gcggacgtca ggcgcatgct gctgctgcag
      961 aaggcctatg ccgaaggcgc gctggcgctg gcgctgtact cggcgcgcct gctcgacgaa
     1021 cagcttgtcg gcgacgaggc ttgcgccgcc cgcgccagcc gcctgctcga cctgctcacg
     1081 cccgtggtca aggcctggcc gtccgaatac gggctggccg cgaacgacct cgccatccag
     1141 atccacggcg gctacggcta cacccgcgac tacgacgtcg agcagctcta ccgcgacaac
     1201 cgcctcaacc cgatccacga gggcaccacc ggcatccagg cgctggacct gctggggcgc
     1261 aagctgctgc gcgaccgtgg cgccgcgctg gccgaactgc gcgcgctggt gtcggcgacg
     1321 gtggccgccg ccgatgcgga tcctgcgcag gccaggcacg cgaagcagct cgacaccgcg
     1381 tggaagcgcg tgggcgaggt agtggacacg ctgctcgaag ccgatgcagc gcacgtcctc
     1441 gacgatgcga cgcccttcct gtgggccttc ggccacgccg tcgtcgcctg gctgtggctc
     1501 gacatggccc gcctgtgcac gggcgacacg cctttccacc gcggcaagcg cgccgccgcg
     1561 cgctgcttct tcgaatacga actgcccagg gtcgacgcct ggctcgacca ggtggccgca
     1621 cgcagcgacc tgctggtgtc caccgcgccc gaactcttcc actcctgagg aaccacgaca
     1681 tgaccgacgt cttcatcgtc tccgccgtgc gcaccgccat cggcaccttc ggcggcgcgc
     1741 tcaaggacac gccgccttcg cagctcggcg tcgccaccgc cagggaagcc atcgcccgcg
     1801 cggggattcc gtttgaggca atcggccacg ccgtctacgg ccaggtgctg ccgagcgaac
     1861 cgcgcgacgc ctacatggcg cgcatcatcg ccatcgagtc cggcctgccg gtggccacgc
     1921 ccgcgctcac cgtcaaccgt ctgtgcggct cgggcctgca ggccatcgtc tcggccgcgc
     1981 agtcgctgct gctgggcgac tgcgacgtgg cgctggccgg cggcgcggaa tcgatgagcc
     2041 gcacgccgtt ctacctcgcg acgcaccgct ggggccgcaa gctcggcgac gcgaccctgg
     2101 tcgacggact caacgagggt ctgaaggatc cgttcaacca ggtgctgatg ggcatcaccg
     2161 ccgagaacat cgcgcagcgc cacgggatcg accgcgcgcg gcaggacgcg atcgcgttgg
     2221 aaagccatcg gcgcgcgacc gccgccatcg cgcggggacg tttcgacggc cagatcgtgc
     2281 cggtgacggt gggcagacac gacaaggaaa ccgaattccg cagcgacgaa cacgtgcgcg
     2341 cccaactctc ggccgacgac ctcgccggcc tgcgaccggt cttcaagccc gatggcagcg
     2401 tcacggcagg caacgcttcc ggcatcaacg acggcgccgc ttcgctggtg ctggccaccg
     2461 gcgaggccgt cgagcgacat ggactcaagc cgctcgcacg cctgatcgcc tatgcgcacg
     2521 ccggcgtcga gccgctgtac atgggcatgg gcccggtgcc ggccacgcgc gcggcgctgg
     2581 cgcgtgcagg attgagcgtc aacgacctcg acgtgatcga atccaacgaa gccttcgccg
     2641 cgcagtgcgg cgccgtgtcc gacgaacttg gactggaccc ggcgaaggtc aatcccaacg
     2701 gcagcggcat cgcgctcggc catccggtcg gcacgaccgg cgcgatcatc accgtcaagc
     2761 tggtgcacga gctgcagcgc agcggcggcc gctacggact ggccacgatg tgcatcggtg
     2821 gcggccaggg tatcgccgcg atcttcgagc ggacctgatc cgcagccgtc catcgaccca
     2881 accccgcacc gtaggagcgg cttcagccgc gatcaccccg acagcctcgt cggaccctcg
     2941 gcacccgttc gcggctgaag ccgctcctgc agtgcacgga ccgggccatg acagaaccga
     3001 agggatgaat gacgccgccg gtgccggtca gtgcagcccg ggggcgctgc cgagggcaac
     3061 gatcttcgcc gaggggcgca ggtgctggcg cagcaggtcc gcagcgaagt ggaagtcgcg
     3121 gcccaggtcc ttgcgggaga agggcgtttc cagcgtgcgc agggccacct cgaacgcggg
     3181 caggtcgccg tgcgcgaact gcgaccagac ctcgaacctg cgctggttca tcgccgccgt
     3241 ccagctgcgg tcgcggttgc gcgcgtaggc gaacggttcg gccagcgcgt cgagttcggc
     3301 ctgcgccagc gcgccgtcga tgccgcaggc ctgcagcagc gcggccagct gcgcggccgc
     3361 catcacgtgc agctgcttca ggtccgccgc gcgcgccggc agcaccagcg aggtgccggt
     3421 ggccgcgttc atcagcaccg cgaacggctg gcggtcgatg aagtcgatgt cgccatgcca
     3481 cggccccagc ggattgccgg ccggcggcgg ctccggcggc gtgggcggct ggcgcaggcg
     3541 cttgaggagt ttttgcgagc agtgcaggac ggtcatggtc aagcgccggt ccggtggcgc
     3601 cagtagcctg ggcgctgatg gcaatgcatg accgccagca ccagcacgcc atcgggttcc
     3661 accgagtaca ggagggcata cgggaagacc cggaccagct tgcggcgcac gtcctgcgcg
     3721 agggcgggcc aggtcagcgg ggcgtggagg atagtgtcga gcgccagctc gacggcgccg
     3781 acgaaccggt tgcccaggcc cgcctgctgc tgttcgtagt agcgcgcggc cgcctcgaac
     3841 tcgtcgagcg cttccgggtg gaacgcaagg cgcgtcactt gagcccggaa cggactcggg
     3901 cgaacacctc gtcggccggg acggccttga ccttgccgga acgcacttcg tcgaggcggt
     3961 gcagcgactc cgccgcccag gcgtcgcgga tctccggatc ggtgagcgga tccaggcttt
     4021 cgaccagccg gtcggccagc agcgcccgcg cgtcgctggg cagggcgagg gcggcttcgg
     4081 caatctgttc gatcgacacg ttcatggccg cagcttaccc cccgggaagg tcatcggcta
     4141 ggctaacggg cggggcgcgg ggcgcccagc atggacaaga gaccgcgatg cccgaactca
     4201 actggctggg cgaccatcag gcccgccgca gcgcgcgccg cgtggcgtac cggctgctgg
     4261 aaaccgtcga acaggtcggc gccgccgacg acggccagcg cgacaacctg ctcatccagg
     4321 gcgacaacct ggaagcgctg aagagcctgc tgccgctgta cgccggccgc gtcaagtgca
     4381 tcttcatcga cccgccgtac aacacgaaga gcgcgttcga gcactacgac gacaacctcg
     4441 agcacagcca gtggctgagc atgatctatc cgcggctggt gctgctgcgc gaactgctgg
     4501 ccgaggacgg cagcatctgg gtgaccatcg acgacaacga ggcgcattac ctcaaggtgt
     4561 tgatggatga ggtgtttggg cggggaaatt tcgtaacatc gtgcacatgg cagcagcgaa
     4621 caactagaga gaaccgaaag gtattttccg aggattgtga aagcattctt ctgtattgcc
     4681 gtgaccctga attattcaaa gcaaagcgca acgatttgga tctcaccgag gcggtcaagg
     4741 ctcgttacaa gaatcctgat gatgatcctc gcggcccatg gcaatcggtt tcgctgaatg
     4801 cccaaggagg acatggaaca ccaagccagt tctatgagtt catcgcgccc agcggcaagg
     4861 tgcacaggct tcccgcaggt cgctgctggc tttacacatg gcctgtcatg caggaaaaga
     4921 ttgcggacag ccgtgtttgg ttcggtgcgg agggaaataa tgcgccgcga gagaagaaat
     4981 tcctgtctga ggccaaggct ggcctaacgc cgaacacact gtggcttgca gctgatgtcg
     5041 gaacgaatga cgaggctaag aaggaaacgg ttaccttgtt cggcgaggac gtcttcgaga
     5101 cccccaaacc cgaacgcctg atccaacgca tcctccacat cgccaccaac gccaacgacc
     5161 tcgtcctcga cagcttcctc ggctccggca ccaccgccgc ggtcgcgcac aagatgggc
//
LOCUS       DAOMPG010000174         5147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6805_length_5147_cov_4.379419, whole genome shotgun sequence.
ACCESSION   DAOMPG010000174 DAOMPG010000000
VERSION     DAOMPG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_5147_cov_4.379419"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1009)
                     /locus_tag="PLS34_09495"
     CDS             complement(95..1009)
                     /locus_tag="PLS34_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HRP87741.1"
                     /translation="MRQLTTLIQRELIENRSSWLVTTVFGGLFVLAALLSVFGLVRVD
                     AFSDPMTLAEIGERVDAVVLGEVLTVLLVAIAGVLSVVTALVVVFYFLDALYAERKDR
                     SILFWKSLPVSDLKVVASKYLTGALTIPLLTVLVFIATAVLVSLVGSVALFRLGGGEL
                     LAAAPLAIGRVALFMLYLIACQVLWFAPVAGWLLLVSAFARRAVLGWAVLPPTLVVVA
                     ERILFGTRHALELIGHRLAGGFELAVQHAERRIIIADDAVMSAFPALADMLTPGRLLA
                     APSLWIGIAVGVAFFAGAVWLRRWRDET"
     gene            complement(1006..1863)
                     /locus_tag="PLS34_09500"
     CDS             complement(1006..1863)
                     /locus_tag="PLS34_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP87742.1"
                     /translation="MNPVIRAQGLSRRYGSTTALDAVDFEIPAGRIVGLIGPNGAGKT
                     TALKAILGLGPFEGELSVLGLDPRRQRAELMQQVSFIADVAVLPRWLRVRQAIDITSG
                     LHPRFSRDKAEAFLDQTEIKRDSKIGALSKGMVTQLHLALVMAIDARLLVLDEPTLGL
                     DIIYRKDFYAALLNDYFDGERTILLTTHQVEEVEHILTDLMFISHGRIVLNESMDTLG
                     EKFLELATRPDRAAEARSLGPLSERQGLGRHYFVFEDRPRDELAPLGEIKVPGVADLF
                     VAKVRRAAS"
     gene            complement(1860..2243)
                     /locus_tag="PLS34_09505"
     CDS             complement(1860..2243)
                     /locus_tag="PLS34_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRP87743.1"
                     /translation="MEGEWNDAQPIYRQLRDRVVTMILEGVLADGDALPSVRTVAAEY
                     RLNPLTVLKGYQELVDEGLVEKRRGRGMFVRDGAGKALLKDERQAFLTEQWPQVVSTI
                     QRLDLDLDELLARARAQMDDKEDMQ"
     gene            2527..3504
                     /locus_tag="PLS34_09510"
     CDS             2527..3504
                     /locus_tag="PLS34_09510"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRP87744.1"
                     /translation="MTQPARVAITGAAGQIGYQLAFRIASGQLLGPNTPVILQLLEIP
                     PALGALQGVEMELLDCAFPTLAGVVATDQADVAFGDADYALLVGARPRGPGMERKDLL
                     MENAKIFSAQGKALNDNAHRKVKVLVVGNPANTNALIALSNAPDLDHARFTAMTRLDH
                     NRAISQLAAKTGASVNDIRQMTVWGNHSATQYPDISHCTVGGKPAPGLVDERWVREDF
                     IPTVQQRGAAIIKARGLSSAASAASAAIDHVRDWALGSAPGDWVSMAVPSDGSYGISE
                     GIVYSFPVRCANGRYEIVQGLEVSDFSRARMTVTEQELLEERNDVRELL"
     gene            3658..>5147
                     /locus_tag="PLS34_09515"
     CDS             3658..>5147
                     /locus_tag="PLS34_09515"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRP87745.1"
                     /translation="MSTLLWFLLFFGGALALAYQRVGLGRATAFAGGALLVYTMFGGG
                     GFAWKLLLWVLFGGMVALNLTDVRRERITRPLLKMYRKMLPSMSRTEREALEAGTVWW
                     EGELFAGVPKWRKLLGAPAPQLSEEEQAFLDGPCEELCRMIDEWQVTHTLQDLSPETW
                     KFLRSKGFFAMIIPKSYGGLEFSAFAHSQVLTKIASRSATAASTVAVPNSLGPAELLL
                     KYGTEEQKNRWLPGLADGREIPCFGLTSPRAGSDAAAIPDSGVVVRRKVDGEDVLGLQ
                     LNFSKRYITLAPVATVVGIAFRMLDPDGLLGKEKDLGITVALVPADTPGVEIGRRHLP
                     LSVPFMNGPIEGRDVFLPLDAIIGGPKMAGQGWRMLMECLSVGRCISLPSNANGGAKA
                     GVFATGAYARIRKQFGMPIGRFEGVQEALARMAGFAYIVDAARSVTIAAVDAGEKPAV
                     PAAILKYHCTEMARVVANDAMDIHGGKAICMGPGNYLGTGYNSIPV"
BASE COUNT          849 a         1791 c         1704 g          803 t
ORIGIN      
        1 gtcgcttgtt tgaataattc atcagttgct ctctgtcatc gattggttga ttgagttgcg
       61 ccactgagct gcgccgtggc acgccgtggc tgcttcaggt ctcgtcccgc cagcggcgca
      121 gccacaccgc cccggcgaag aacgccacgc cgaccgcgat gccgatccac agcgacggcg
      181 ccgccagcag ccgccccggc gtcagcatgt ccgccagcgc ggggaaggcc gacatcacgg
      241 cgtcgtccgc gatgatgatc cgccgctcgg cgtgctgcac ggccagttcg aagccgcccg
      301 ccaggcggtg gccgatcagc tcgagcgcgt ggcgcgtgcc gaacaggatg cgctcggcga
      361 ccacgacgag ggtcggcggc agcaccgccc agcccagcac ggcgcgcctg gcgaaagcgg
      421 aaaccagcag cagccagcct gcgacgggcg cgaaccacag cacctggcag gcgatcaggt
      481 acagcatgaa cagcgcgacc cgcccgatgg cgagcggcgc ggccgccagc aactcgccac
      541 cgccgagccg gaacaacgcg acgctgccga ccaggctcac cagcacggcg gtggcgatga
      601 acaccagcac cgtcagcagc gggatggtca gcgcgccggt caggtacttg gaggcaacca
      661 ccttcaggtc cgacacgggc agcgacttcc agaacaggat gctgcggtcc ttgcgctccg
      721 cgtacagcgc atcgaggaaa tagaacacca ccacgagcgc cgtcaccacg ctcagcacgc
      781 cggcgatcgc caccagcagc accgtcagca cctcgccgag caccacggcg tcgacgcgtt
      841 cgccgatctc ggcgagcgtc atgggatccg agaaggcgtc cacgcgcacc aggccgaaca
      901 ccgacagcag ggccgccagc acgaacaggc cgccgaacac cgtggtcacg agccagctcg
      961 agcggttctc gatcagctcc cgctggatca gggtcgtcag ctgtctcatg acgctgccct
     1021 cctgaccttg gcgacgaaca ggtcggccac cccggggacc ttgatttcgc ccagcggcgc
     1081 aagctcgtcg cgcgggcgat cctcgaacac gaaataatgc cgcccgaggc cctggcgctc
     1141 cgacagcgga ccgagactgc gcgcctcggc cgcgcggtcg ggccgcgtcg ccagctcgag
     1201 gaacttctca cccagcgtgt ccatgctctc gttgagcacg atgcggccgt ggctgatgaa
     1261 catcaggtcc gtgaggatgt gctcgacctc ctcgacctgg tgcgtggtca gcagaatggt
     1321 gcgctcgccg tcgaaatagt cgttcagcag cgcggcatag aagtccttgc ggtagatgat
     1381 gtcgaggccc agcgtgggct cgtcgagcac cagcaggcgc gcgtcgatgg ccatcaccag
     1441 tgcgaggtgc agctgcgtga ccatgccctt ggacaacgcg ccgatcttgc tgtcgcgctt
     1501 gatctcggtc tggtcgagaa aggcctcggc cttgtcacgg ctgaagcggg gatgcaggcc
     1561 ggaggtgatg tcgatggcct ggcgcacgcg cagccagcgc ggcagcacgg cgacatcggc
     1621 gatgaacgac acctgctgca tgagctccgc ccgctggcgg cgcgggtcga gcccgagcac
     1681 ggagagttcg ccctcgaagg gcccgagccc caggatggcc ttcagggccg tggtcttgcc
     1741 ggcaccgttc ggcccgatca gcccgacgat gcgcccggcc ggaatctcga agtcgacggc
     1801 gtcgagcgcc gtggtgctgc cgtagcggcg cgacaggccc tgggcacgaa tcacgggatt
     1861 cactgcatat cctccttgtc gtccatctgc gcgcgcgccc gcgccagcag ctcgtcgaga
     1921 tcgaggtcga gacgctggat ggtcgaaacc acttgcggcc actgctcggt gaggaacgcc
     1981 tgccgctcgt ccttgagcag cgccttgccg gccccgtcgc gtacgaacat gccgcgcccg
     2041 cggcgtttct ccaccagccc ctcgtcgacg agttcctggt agcccttgag cacggtgagc
     2101 gggttcagcc ggtattcggc ggccacggtg cgtaccgaag ggagcgcgtc accgtcggcg
     2161 agcacgcctt cgagaatcat ggtcaccacc cggtcgcgaa gctgccggta gatgggctgg
     2221 gcgtcgttcc attcgccttc catggtgctc tccgcgcgcc tcaggccatc atcgaggcaa
     2281 gcgtgaggca ggcgatggcg acgatggtca gcatccgcat ggcctgttcg tgcttactgg
     2341 cgttgtttgg tttgttcatg ttcatgacct gcctcgatga cggctgtccg tcgctggctg
     2401 agccggtgtt gtagtgaact ataacaccag tttcgggaat tgcaaccccc cccgacccat
     2461 gccgcccccg acgcgccgtc gccgctgcta aaatgccgcg tcccgccgaa ttccaggaac
     2521 ccacagatga cccaacccgc gagagtcgcc atcaccggcg ccgccggcca gatcggatac
     2581 cagctggctt tccgcatcgc ctccggccag ctgctcggcc cgaatacccc cgtgatcctg
     2641 cagctgctcg agatcccgcc cgcgctcggt gcgctgcagg gcgtggagat ggaattgctg
     2701 gactgcgcct ttcctacgct cgccggcgtg gtggccaccg accaggcgga cgtggccttc
     2761 ggcgacgccg attacgccct gctggtcggc gcccggccgc gcggccccgg catggagcgc
     2821 aaggacctgc tcatggagaa cgccaagatc ttctccgcgc agggcaaggc gctgaacgac
     2881 aatgcgcacc gcaaggtcaa ggtgctggtg gtcggcaacc cggccaacac caatgcgctg
     2941 atcgcgctct ccaacgcccc ggacctggac cacgcacgct tcaccgcgat gacccggctg
     3001 gatcacaacc gcgccatcag ccagctcgcc gccaagaccg gcgcgagcgt caacgacatc
     3061 cgccagatga ccgtctgggg caatcattcc gccacccagt atcccgacat cagccactgc
     3121 acggtcggcg gcaagcccgc accgggcctg gtggacgagc gctgggtgcg cgaggacttc
     3181 attcccaccg tgcaacagcg cggcgcggcc atcatcaagg cgcgcggcct gtcttccgcc
     3241 gcctcggccg cctcggcggc gatcgaccac gtgcgcgact gggcgctcgg cagcgcgccg
     3301 ggcgactggg tcagcatggc ggtgccgtcc gacggcagct acggcatcag cgaaggcatc
     3361 gtgtattcct tcccggtgcg ttgcgcgaac ggccgctacg agatcgtcca ggggctggag
     3421 gtcagcgatt tcagtcgcgc gcgcatgacc gtcaccgagc aggagctgct cgaggaacgc
     3481 aacgacgtgc gcgagctgct gtaagcgcgg ccgccggcgg gcccggccga tcaaggggac
     3541 attgccggcg agcaatgtcc cctttttgtt gccggagccc aacggagtta tattcaaaca
     3601 actgtatgcc gccgcgatgc gtgcacggcg gcccgcttgc tcgaggggtg atccatcgtg
     3661 tcgactctgt tgtggttcct gttgttcttc ggcggcgcgc tggcgctcgc ctaccagcgt
     3721 gtcggactgg ggcgtgccac cgccttcgcc ggcggggcgc tgctggtcta cacgatgttc
     3781 ggcggcggcg gattcgcctg gaaactgctg ctgtgggtgc tgttcggcgg catggtggca
     3841 ttgaacctga ccgacgtgcg gcgcgagcgc atcacccggc cgttgttgaa gatgtaccgc
     3901 aagatgctgc cgtcgatgtc gcgtaccgag cgcgaggcgc tcgaagccgg caccgtctgg
     3961 tgggaaggcg agctgtttgc cggcgtgccg aaatggcgca agctgctggg cgcgccggcg
     4021 ccgcagctgt ccgaagagga gcaggctttc ctcgacggcc cctgcgagga gctctgccgg
     4081 atgatcgacg aatggcaggt cacgcacacc ctgcaggacc tttcgccgga gacctggaag
     4141 ttcctgcgca gcaagggctt cttcgcgatg atcatcccga agagctacgg cggcctcgag
     4201 ttctccgcct tcgcgcacag ccaggtcctc accaagatcg ccagccgcag cgcgaccgcc
     4261 gcctccacgg tcgcggtgcc gaactcgctg ggcccggccg agctgctgct gaagtacggc
     4321 accgaggagc agaagaaccg ctggttgccc gggctggccg acgggcgtga gatcccctgt
     4381 ttcggcctga ccagcccgcg tgccggctcc gacgccgccg ccatcccgga cagcggcgtg
     4441 gtggtcaggc gcaaggtgga cggcgaggac gtgctcggcc tgcagctcaa cttctcgaag
     4501 cgctacatca ccctcgcgcc ggtcgcgacg gtggtcggca tcgccttccg catgctcgat
     4561 cccgacggcc tgctcggcaa ggagaaggat ctcggcatca cggtggcgct ggtgccggcg
     4621 gacacgccgg gcgtcgagat cggccgtcgc cacctgccgc tgtcggtgcc gttcatgaac
     4681 ggcccgatcg agggccggga cgtattcctg ccgctggacg cgatcatcgg cgggccgaag
     4741 atggccggcc agggctggcg catgctgatg gaatgcctct ctgtgggccg ttgcatctcg
     4801 ctgccgtcca acgccaacgg cggcgccaag gccggggtat tcgccaccgg cgcctacgcg
     4861 cgcatccgca agcagttcgg catgccgatc ggccgcttcg agggcgtgca ggaagcactc
     4921 gcgcgcatgg cgggcttcgc ctacatcgtc gacgccgcac gcagcgtcac catcgccgcc
     4981 gtcgacgccg gcgagaagcc ggccgtgccg gccgccatcc tcaagtacca ctgcaccgag
     5041 atggcccggg tggtcgccaa cgatgccatg gacatccacg gcggcaaggc catctgcatg
     5101 ggtccgggca actacctggg caccggctac aactccatcc cggtgat
//
LOCUS       DAOMPG010000175         5123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6851_length_5123_cov_5.206985, whole genome shotgun sequence.
ACCESSION   DAOMPG010000175 DAOMPG010000000
VERSION     DAOMPG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_5123_cov_5.206985"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /gene="glyS"
                     /locus_tag="PLS34_09520"
     CDS             <1..1970
                     /gene="glyS"
                     /locus_tag="PLS34_09520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRP87746.1"
                     /translation="LGHGEMESFATPRRLAVKVRRLATRQADQQVVLRGPPAGVARAD
                     GTWTKAAEKFAASAGVPVESLVEQAEGKGVYVYAHKTEHGAAAGDLLPGLVEAALAAL
                     PVPRRMRWGASDAEFVRPVHWLVMLLGKDVVPCALFGAVAGRNTRGHRFMAPVPVELA
                     GPEDYPARLESEGFVVAGFAARRERIRAQAETAASEHGGRLVLDPSLLDEVTALVEWP
                     VPVTGAFEARFLELPEEVLIATLQDHQRYFPMRGPDGRLLAAFITISNLDSPEPAAIR
                     HGNERVVRPRLADAAFFWAQDRRAPLQARTALLDGVVFQKKLGSVGDKSRRVAALAAH
                     VAELLGDDARAAEHAALLAKCDLVTAMVGEFPELQGIMGGYYARADGQPEELARAIEE
                     QYLPRFAGDRLPATRLGQVLALADKLDTIAGIFAIGQRPTGNRDPFGIRRAALGVLRI
                     LIECELDLDLPALLARAVDGQPVRRNDDDAKGILEFLLERLRGYYLEGAGSIVAPHDI
                     FEAVLARRPTSLVDFHLRLIAVQEFARLDAAVALAAANKRIANILRQVDAGQLGKVDP
                     SLLREDAERALHQQLALLADEVAPLVATRRYRPALERLATLREVIDRFFDDVLVMDED
                     QALRSNRLSLLASLRALFMDVADVSRLGGSA"
     gene            1986..2717
                     /locus_tag="PLS34_09525"
     CDS             1986..2717
                     /locus_tag="PLS34_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRP87747.1"
                     /translation="MIWLRSLLFTAFLFLSVPVYATGVLLLAPFGHRVYYRSAVGWAR
                     ANLWLLDRLCGLRFEVEGLENIPQSPCVLYWKHESVWETMAGAVLFPPQTWVVKRELM
                     WLPFFGWGLALLKPIAINRKAGRTAVKQVMSQGRERLANGLFVVIYPEGTRVLPGQER
                     RFGISGAALAKSAGRPILPVAHNAGDFWPRRSFLKRAGVIKVVIGAPIETGELSNEEI
                     TARGREWIAAAIERISPHRGVAGGS"
     gene            complement(2787..>5123)
                     /gene="gyrB"
                     /locus_tag="PLS34_09530"
     CDS             complement(2787..>5123)
                     /gene="gyrB"
                     /locus_tag="PLS34_09530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRP87748.1"
                     /translation="MYIGDTDDGTGLHHMVFEVVDNSIDEALAGYCSTITVIIHADES
                     VTVADDGRGIPVDMHPEEKCSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNAL
                     SEHLDLTIHRDGKVHRQEYQLGKPLYDLKVLDDTTRTGTVIRFKPSAEVFSNIDFHYD
                     ILAKRLRELSFLNSGVRIVLQDERDGQEDVFEYEGGIRAFVEHLNRNKTPVHPSVFYF
                     QNERDGIQVEVAMQWNDSYQESMFCFTNTIPQRDGGTHLAGFRSALTRTLNGYIEREN
                     VARKEKVSTTGDDAREGLTAVLSVKVPDPKFSSQTKDKLVSSEVKSAVESLMAEKLEE
                     FLLEKPADAKAIVGKLIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAL
                     SELFLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLSSAEVGTLITALG
                     CGIGREEFNIDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIAQPP
                     LYKIKKGKQEHYVKDDAELLAYLSSMALDEAALYLAPGAPPLQGPGLESLMKKYTEVM
                     AIIHRLGRRYDERVLEQLLYSAPLTAAELDDLGRVDAWMTTLARRLAGPADAPRYELS
                     LVHEADRPGWEIQVRRRHHGVDTVRQLQRDFFESAEYRRIAGLGSELADLVGEGAYVM
                     RGDKRQPVASFREAVEWLMAEARKGQAIQRYKGLGEMNPDQLWDTTVNPETRRLMQVR
                     IEDAVAADDIFTTLMGDQVEPRREFIEKNALTVSNLDV"
BASE COUNT          752 a         1707 c         1785 g          879 t
ORIGIN      
        1 gcctgggcca cggggagatg gaatccttcg caacgccgcg ccggctggcc gtgaaggttc
       61 gtcggctggc gacgcgccag gccgatcagc aggtcgtgct gcggggaccg cccgccggcg
      121 tggcgcgtgc cgacggcacc tggacgaagg cggcggagaa gttcgcggcc tcggccggcg
      181 tgccggtcga gtcgctggtg gaacaggccg agggcaaggg cgtctacgtc tacgcgcaca
      241 agaccgagca cggcgcggcc gccggggacc tcctgccggg gctcgtggag gccgcgctgg
      301 cggcgctgcc ggtgccgcgg cggatgcgct ggggtgcgtc cgacgccgag ttcgtgcgcc
      361 cggtacactg gctggtgatg ctgttgggca aggacgtcgt gccttgcgcg ctgttcggcg
      421 ccgtcgccgg ccggaacacg cggggtcatc gcttcatggc gccggtgccg gtggagctgg
      481 ccggcccgga ggactatccg gcgcggctcg agagcgaagg cttcgtggtg gccggcttcg
      541 ccgcccgccg cgagcgtatc cgcgcccagg ccgagaccgc ggcgagcgag cacggcggcc
      601 ggctggtgct cgatccgtcg ctgctcgacg aggtcacggc gctggtggaa tggcccgtgc
      661 cggtgacggg cgcgttcgag gcgcgcttcc tggagctgcc ggaagaagtc ctgatcgcca
      721 ccttgcagga ccaccagcgc tatttcccga tgcgcgggcc ggacgggcga ctgctggcgg
      781 cgttcatcac catcagcaac ctggacagtc cggagcctgc cgcgatccgg catggcaacg
      841 agcgcgtcgt gcggccgcgc ctcgccgatg cggcgttctt ctgggcgcag gaccggcgcg
      901 cgccgctgca ggcccgcacg gcgctgctcg acggcgtggt gttccagaag aagctcggca
      961 gcgtcggcga caagagccgc cgcgtcgccg cgctggccgc gcacgtcgcc gaactgcttg
     1021 gcgacgacgc gcgggccgcc gagcatgccg cgctgctcgc caagtgcgac ctcgtcaccg
     1081 cgatggtcgg cgagttcccc gagctgcagg gcatcatggg cggctactac gcgcgtgccg
     1141 acgggcaacc cgaggagctc gcgcgcgcca tcgaggaaca gtacctgccg cgatttgccg
     1201 gcgaccgcct gcccgcgacg cgtctcggcc aggtgctggc actggccgac aagctcgaca
     1261 ccatcgccgg gatcttcgcc atcggccagc gccccaccgg caaccgcgat cccttcggga
     1321 tccgccgcgc cgcgctgggc gtgctgcgca tactcatcga gtgcgagctc gacctcgacc
     1381 tgcccgcgct gctggcgcgg gcggtggacg gacagccggt gcgccgcaac gacgacgacg
     1441 ccaaggggat cttggaattc ctgctcgagc ggctgcgtgg ttattacctc gagggcgccg
     1501 ggtccatcgt cgcgccgcac gacattttcg aagcggtgct cgcgcggcgc ccgacgtcac
     1561 tggtggactt ccacctgcga ctgatcgccg tgcaggagtt cgccaggctg gatgccgcgg
     1621 tcgcgctggc agccgcgaac aagcgcattg ccaacatcct gcgtcaagtg gatgccgggc
     1681 aactcggcaa ggtggatccg agcctgctgc gggaggatgc tgagcgcgcg ctgcaccagc
     1741 aactggccct cctggcggat gaagttgcac cgctggtggc cacgcgccga taccggccgg
     1801 cactggagcg gctggccacc ttgcgcgagg tcatagatcg cttcttcgac gacgtgctgg
     1861 tgatggacga ggatcaggcg ctgcgcagca accggctgtc gctgctggcg agtctgcgcg
     1921 cgctgttcat ggacgtcgcg gatgtgtcga ggctcggtgg ctcggcctga gcgggagcgc
     1981 cgccggtgat ctggctgcgg tcgctgctgt tcacggcgtt cctgttcctg tcggtgccgg
     2041 tgtacgcgac cggcgtgctg ctgctggccc cgttcggcca ccgggtgtac taccgcagcg
     2101 cggtcggctg ggcgcgcgcc aacctctggt tgctggacag gttgtgcggc ctgcgtttcg
     2161 aggtcgaggg cctggagaac atcccgcagt cgccctgcgt gctctactgg aaacacgagt
     2221 cggtgtggga aaccatggcc ggcgccgtac tgtttccgcc gcaaacctgg gtggtgaaac
     2281 gcgaactgat gtggctgccg ttcttcggct ggggcctggc attgctcaag cccatagcca
     2341 tcaatcgcaa ggccgggcgg accgcggtga agcaagtgat gtcgcaaggc cgcgagcgcc
     2401 tggccaacgg cctgttcgtg gtgatctacc cggagggtac ccgcgtgctg ccgggccagg
     2461 aacggcgctt cggcatcagc ggtgcggccc tcgccaagtc agcaggtcgg ccgatcctgc
     2521 cggtcgcgca caacgccggc gacttctggc cacggcgcag cttcctgaaa cgtgcgggcg
     2581 tcatcaaggt ggtgatcggc gcgccgatcg agaccggcga gctcagcaac gaggaaatca
     2641 ctgcccgtgg tcgcgagtgg atcgcagccg ccatcgagcg catcagtccg catcgggggg
     2701 ttgccggcgg atcctgacgc gaccgccgag cctctggaac ggcggctacc tcgccccgat
     2761 aaagggtgaa aatgcgccta tcttcgtcaa acgtccaggt ttgagaccgt caaggcattc
     2821 ttctcgatga actcgcgacg cggttcgacc tggtcgccca tcaaggtcgt gaagatgtcg
     2881 tccgcggcca ccgcgtcctc gatgcgcacc tgcatgagac ggcgggtctc cggattcacg
     2941 gtggtgtccc agagctggtc gggattcatt tcgccaaggc ccttgtagcg ctggatcgcc
     3001 tggcccttgc gcgcctccgc catcagccat tcgaccgcct cgcggaagct ggcgaccggc
     3061 tggcgcttgt cgccgcgcat gacgtacgcg ccctcgccga ccaggtcggc cagctccgaa
     3121 ccgaggccgg cgatgcgccg gtattccgcg gactcgaaga aatcgcgttg cagctggcgc
     3181 accgtgtcca cgccatggtg gcggcgccgc acctggatct cccagcccgg ccggtccgcc
     3241 tcgtgcacca gcgacaactc gtagcgcggc gcgtcggccg gaccggccag ccgccgcgcc
     3301 agcgtggtca tccaggcatc gacccggccc aggtcgtcca gctcggcggc cgtgagcggc
     3361 gcactgtaga gcaattgctc gagcacccgc tcgtcgtaac ggcgaccgag gcggtggatg
     3421 atggccatca cctcggtata tttcttcatc aggctctcga ggcccggtcc ctggagcggc
     3481 ggcgcgcccg gcgccaggta cagcgcggcc tcgtccagcg ccatgctgga caggtaggcg
     3541 agcagctccg catcatcttt tacataatgc tcctgcttgc ccttcttgat cttgtacagc
     3601 ggcggctgcg cgatgtagat gtggccgcgc tcgatcagct ccggcatctg gcggtagaag
     3661 aaggtcaaga gcagcgtgcg gatgtgcgag ccgtcgacgt cggcgtcggt catgatgatg
     3721 atgcggtggt agcgcagctt gtcgatgttg aactcttcgc gcccgatgcc gcagcccagc
     3781 gcggtgatca aggtgccgac ctcggccgag gacagcatct tgtcgaagcg ggctttctcg
     3841 acgttcagga tcttgccctt cagcggcagg atcgcctgcg tgcgccggtc gcggccctgc
     3901 ttggcggagc cgccggccga gtcgccctcg accaggaaca gctccgagag cgccgggtcc
     3961 ttctcctggc aatccgccag cttgccgggc aggccggcga tgtccagcgc gcccttgcgc
     4021 cgggtcatct cgcgcgcctt gcgggcggcc tcgcgcgccc gtgcggcatc gatcagcttg
     4081 ccgacgatcg ccttggcgtc ggccggcttc tcaagcagga attcctcgag cttctcggcc
     4141 atgagcgact cgaccgcgct tttcacctcg gacgagacca gcttgtcctt ggtctgcgag
     4201 gagaacttcg gatcgggcac cttcaccgac aagaccgcgg tcagtccctc gcgggcgtcg
     4261 tcgccggtgg tgctgacctt ttccttgcgg gcgacgttct cccgctcgat gtagccgttg
     4321 agcgtgcgcg tcagggcgct gcgaaagccg gccaggtggg tgccaccgtc gcgctgcggg
     4381 atggtgttgg tgaagcagaa catgctctcc tggtaggagt cgttccactg catggccacc
     4441 tcgacctgga tgccgtcgcg ctcgttctgg aaatagaaca ccgacggatg caccggcgtc
     4501 ttgttgcggt tgaggtgctc gacgaaggcg cgaatgccgc cctcgtactc gaacacgtct
     4561 tcctggccgt cccgctcgtc ctgcagcacg atgcgcacgc cggaattcag gaaggacagc
     4621 tcgcgcagcc gcttggcgag gatgtcgtag tggaaatcga tgttactgaa cacctcggcg
     4681 ctcggcttga agcggatcac cgtgccggtc cgcgtcgtat cgtcgagcac cttcaaatcg
     4741 tacagcggct tgccgagctg gtattcctgg cgatgcacct tgccgtcgcg atgaatggtg
     4801 agatccaggt gctccgacag ggcgttcacc acggacacgc cgacgccgtg gagcccgcca
     4861 gacaccttgt aggaattgtc gtcgaacttc ccgccggcgt gcagcaccgt catgatcacc
     4921 tgggcggcgg agcacttctc ctcgggatgc atgtcgaccg ggatgccgcg gccgtcgtcc
     4981 gccaccgtca ccgactcgtc ggcgtggatg atgacggtga tggtggagca atagcccgcc
     5041 aaggcctcgt caatggagtt gtccacgacc tcgaacacca tgtggtgcag gccggtgccg
     5101 tcatcggtgt cgccgatgta cat
//
LOCUS       DAOMPG010000176         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6885_length_5099_cov_4.680214, whole genome shotgun sequence.
ACCESSION   DAOMPG010000176 DAOMPG010000000
VERSION     DAOMPG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_5099_cov_4.680214"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLS34_09535"
     CDS             <1..301
                     /locus_tag="PLS34_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87749.1"
                     /translation="EEPFMAGLRQLGVLGASPRGRIAPGRIDPDGVAWPVPIETLQPA
                     DLEAVERDWAAPLLAHLQRGRVAGVRIVTGRRVHETRRGDALRFWRRPQPVGVSC"
     gene            295..1998
                     /gene="recJ"
                     /locus_tag="PLS34_09540"
     CDS             295..1998
                     /gene="recJ"
                     /locus_tag="PLS34_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRP87750.1"
                     /translation="MLSRRPLHRRPVEAAALAALGGIDPVLARLFAARGIRAPGALEL
                     GLAGLAPVGSLEGVEAAAGLVADHLQARRNILVVGDFDADGATSTALLVRVLRALGGE
                     PAYLVPDRFVHGYGLSPALVRDAAAHAPALLITVDSGINCHAGVELARQLGMDVLVTD
                     HHLPGEHLPPATAIVNPALPGQAFPSRCLAGVGVAFYLAAALARRLGADRALVPGLLD
                     LVALGTVADLVPLDRNNRILVEHGLQRIRAGACRPGVTALLAAGKRDPRSAVTTDLAF
                     AAGPRLNAAGRLDDMSVGIECLLSDDAGRAGELAALLSGLNEERKEIEARMQQEALRA
                     VEGLEPGPGELPPVLCVYRKGWHSGVVGLVAARLKERYHRPSVAFAADDDGLLKGSAR
                     SVPGLHIRDALADVDAQHPGLIERFGGHAMAAGLTLAPAGLPEFRRALAAAVAARLEP
                     GALEGAILSDGELPEEAFCLAMAERLRDAAPWGQGFPEPLFDGVFELLDARVVGADHL
                     KLRLRSPGGQRVLDGIAFRQAERLGGARPARLRLAYRLGVNDYAGLRAAQLVVEYLEP
                     A"
     gene            2076..3171
                     /gene="prfB"
                     /locus_tag="PLS34_09545"
     CDS             join(2076..2147,2149..3171)
                     /gene="prfB"
                     /locus_tag="PLS34_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638028.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRP87751.1"
                     /translation="MEISPLRQQLADLASRADALRRYLDYPGKRERLEEVRRELEDPT
                     VWNKPERAQALGRERAQLEGVVEMLDEIGAGVTDAADLLELAAEEHDEATVTGIATDA
                     DALEKKIEELEFRRMFSGPMDEHNAFVDINAGQGGTEAQDWAEMLLRMYLRWIESHGF
                     EAEIIEASAGEVAGIKSATVHVTGPYAYGWLRTETGIHRLVRKSPFDSGNRRHTSFAG
                     VFVSPEVDDEVDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHNPTGIVVQCQND
                     RSQHKNKATAMKQLKAKLYELEMQERRAKAQAVEDTKSDIAWGHQIRSYVLDQSRIKD
                     LRTGVEVGNTAAVLNGDLDQFIEASLKQGL"
     gene            3191..4681
                     /gene="lysS"
                     /locus_tag="PLS34_09550"
     CDS             3191..4681
                     /gene="lysS"
                     /locus_tag="PLS34_09550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRP87752.1"
                     /translation="MSEQPENALIAERRRKLEALREAGEAYANDFRRNALAEELHLAF
                     GELDGEALEREGVRVAIAGRLMAKRVMGKASFARLDDMSGAIQLFMQRDALPEGVYAA
                     FKGWDLGDLVGAEGTLFKTRTGELSVKVEAARLLSKCLRPLPEKYHGLADTETRYRQR
                     YLDLIMNADSRAVFRLRSQMVSFMRSYLDARGFLEVETPMMQPIPGGANARPFITHHN
                     ALGIDMYLRVAPELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPEFTMLELYQAYVD
                     QVAMMDIAEDLLRMMAVALTGGARLAWQGAEVDLGPAFRRVALEELVLEANPALDSLR
                     ARDAGYLREFAAGLGMHVEPGWGAGKLLLELFEKTVEPDLQQPTFVTGYPLEVSPLAR
                     CRDADPFLTDRFELFVCGRELANGFSELNDPEEQARRFRAQVEQKAAGDEEAMFYDAD
                     YVRALEYGMPPAGGLGIGVDRLVMLFADQPSIRDVLLFPQMRPEDT"
BASE COUNT          734 a         1771 c         1866 g          728 t
ORIGIN      
        1 cgaagagccg ttcatggcgg gcctgcggca gttgggggtg ctgggcgcgt cgccgcgcgg
       61 gcggattgcg ccgggccgca tcgatcctga cggcgtcgcc tggccggtgc cgatcgagac
      121 gctgcagccg gccgacctgg aggccgtcga gcgggactgg gcggcgccgc tgctcgccca
      181 cctgcagcgc ggtcgggtgg cgggcgtgcg gatcgtgacc ggccgccggg tgcacgagac
      241 gcgccgtggc gacgccctgc gcttctggcg gcggccgcag ccggtgggcg tgtcgtgctg
      301 agccgccggc cgctgcatcg ccgcccggtc gaggccgccg cgctggccgc gctcggcggg
      361 atcgacccgg tgctggcgcg actgttcgcg gcacgcggca ttcgcgcgcc gggcgcactc
      421 gaactgggcc tcgccgggct ggcgccggtc ggcagcctgg agggggtgga ggccgcggcc
      481 gggctggtgg cggatcacct gcaggcgcgg cggaatattc tcgtggtggg cgatttcgac
      541 gccgacggcg ccaccagcac cgcgctgctg gtgcgcgtgc tgcgcgcact cggtggcgag
      601 cccgcctacc tggtgccgga ccggttcgtg cacggctacg gcctcagccc ggcgctggtg
      661 cgggatgccg ccgcccatgc gccggcgctg ctgatcaccg tggacagcgg catcaattgc
      721 catgccggcg tcgagctggc gcgccagctc ggcatggacg tgctggtcac cgatcatcac
      781 ctgccgggcg agcacctgcc gccggccacc gcgatcgtca acccggcgct gccggggcag
      841 gcgttcccga gccgctgcct ggccggcgtc ggcgtggcct tttacctggc ggcggcactg
      901 gcccgccggc tcggcgccga ccgtgcgctg gtgcccggcc tgctcgacct ggtggcgctc
      961 ggcacggtcg cggacctggt gccgctggat cgcaacaacc ggatcctggt cgagcacggc
     1021 ctgcagcgga ttcgcgccgg cgcgtgccgt ccgggcgtga ccgcgctgct ggcggccggc
     1081 aagcgcgacc cgcgcagtgc cgtcaccacc gacctggcct tcgcggccgg cccgcgcctg
     1141 aacgcggccg ggcgactgga cgacatgtca gtcggcatcg agtgcctgct gagtgatgac
     1201 gccgggcgcg ccggcgagct ggctgcgctg ctgtccggcc tcaacgagga gcgcaaggaa
     1261 atcgaggcgc gcatgcagca ggaagcgctg cgcgcggtcg aaggtctcga acccggtccc
     1321 ggcgagttgc cgccggtgct gtgcgtatac cgcaagggct ggcacagcgg cgtggtcggc
     1381 ctggtggcgg cgcgcctgaa ggagcgctat caccgtccgt cggtggcctt cgcagcggac
     1441 gacgacggcc tgctcaaggg ttcggcgcgc tccgtccccg gcctgcacat tcgcgatgcg
     1501 ctggccgacg tggacgcgca gcatccgggc ctgatcgagc gcttcggcgg gcacgccatg
     1561 gccgccggcc tgacgctggc gcccgccggc ttgccggagt ttcgccgggc gctggctgcg
     1621 gcggtcgccg cgcggctcga gccgggcgcg ctggaaggcg ccatcctgtc cgatggcgaa
     1681 ttgcccgagg aggccttctg cctggccatg gccgagcggc tgcgggacgc cgcgccgtgg
     1741 ggccagggct ttcccgagcc gctgttcgac ggcgtgttcg aactgctgga tgcgcgggtc
     1801 gtcggcgccg accacctcaa gctccgactg cgctcgcccg gcgggcagcg ggtgctcgac
     1861 ggcatcgcct ttcgccaggc cgagcggctc ggcggcgcgc ggccggcgcg cctgcggctg
     1921 gcctaccggc tgggggtcaa cgattacgcc ggcttgcgcg cggcacagct ggtggtcgag
     1981 tatctcgagc ctgcgtgacc ccggcggcag ccgctgttgc gctgcgtgat aaacttccgg
     2041 gttgttcccg cacccgcaac aggccgaacg ccgcgatgga aatctcaccc ctgcgccagc
     2101 aactggcgga tctcgcctcg agggccgatg ccctgaggag gtacctttga ctacccgggc
     2161 aagcgcgagc ggctcgagga agtgcggcgc gagctcgagg atcccaccgt ctggaacaag
     2221 cccgagcggg cccaggcgct aggccgggag cgcgcccagc tcgagggagt ggtcgagatg
     2281 ctcgacgaga tcggcgccgg cgtgaccgat gcggccgacc tgctcgagct ggcggcggag
     2341 gagcacgacg aggctacggt gacgggcatc gccacggacg ccgacgcgct cgagaagaag
     2401 atcgaggagc tcgagttccg gcgcatgttc tccggcccga tggacgagca caacgctttc
     2461 gtcgacatca atgccggcca gggcggtacc gaggcgcagg actgggccga gatgctgctg
     2521 cgcatgtacc tgcgctggat cgagtcgcac ggcttcgagg ccgagatcat cgaggcctcg
     2581 gcgggcgagg tggccggcat caagagcgcc acggtgcacg ttaccggccc ctatgcctac
     2641 ggctggttgc gcaccgagac cggcattcac cgcctggtgc gcaaatcgcc gttcgactcc
     2701 ggcaaccgcc ggcacaccag ctttgccggc gtgttcgttt cgcccgaggt ggacgacgag
     2761 gtcgacatcg agatcaaccc ggccgacctg cgcatcgacg tgtaccgcgc cagcggcgcg
     2821 ggcggccagc acgtcaaccg caccgagtcg gcggtgcgca tcacgcacaa cccgaccggc
     2881 atcgtcgtgc agtgccagaa cgaccgcagc cagcacaaga acaaggccac ggcgatgaag
     2941 cagctcaagg ccaagctcta cgagctggag atgcaggagc gccgcgccaa ggcgcaggcg
     3001 gtggaggaca ccaagtcgga catcgcctgg gggcaccaga tccgcagcta cgtgctcgac
     3061 cagtcccgca tcaaggacct gcgcaccggc gtggaggttg gcaacacggc ggccgtgctg
     3121 aacggcgacc tggaccagtt catcgaggcc agcctgaagc agggtctcta gcccgagacg
     3181 gggaaacatc atgtcggaac agcccgaaaa cgccctgatc gccgagcgcc gccgcaagct
     3241 ggaggcgctg cgcgaggccg gcgaggccta tgccaacgac ttccgccgca acgcgctggc
     3301 cgaggagttg catctggctt tcggcgaact cgacggcgag gcgctggagc gcgagggcgt
     3361 gcgcgtcgcg atcgccgggc ggctgatggc caagcgcgtc atgggcaagg ccagcttcgc
     3421 ccggctcgac gacatgagcg gcgccatcca gctgttcatg cagcgcgacg ccctgcccga
     3481 gggcgtctac gcggcgttca agggctggga cctcggtgat ctcgtcggcg ccgaaggcac
     3541 gctgttcaag accaggaccg gcgagctgtc ggtcaaggtc gaggcggcac gcctgctctc
     3601 caagtgcctg cgccccttgc ccgagaagta ccacggcctg gcggacactg aaacgcgcta
     3661 ccgccagcgc tatctcgacc tgatcatgaa tgccgacagc cgcgccgtgt tccggctgcg
     3721 ctcgcagatg gtcagcttca tgcgcagcta tctcgacgcg cgcggctttc tcgaggtcga
     3781 gactccgatg atgcagccga tccccggcgg cgcgaatgca cgaccgttca tcacccatca
     3841 caacgcgctg ggcatcgaca tgtacctgcg ggtcgcgccc gagctctatc tcaagcggct
     3901 ggtagtgggc ggcttcgagc gcgtctacga gatcaaccgc aatttccgca acgaggggct
     3961 gtccacgcgg cacaaccccg agttcaccat gctcgagctc taccaggcct atgtcgacca
     4021 ggtcgcgatg atggacatcg ccgaggacct gctgcgcatg atggccgtgg ccctgaccgg
     4081 cggcgcccgg ctggcctggc agggcgcgga ggtggatctc gggccggcgt tccggcgcgt
     4141 ggcgctggag gaactggtgc tcgaggcgaa cccggcgctc gactcgctgc gcgcccgcga
     4201 cgcgggctac ctgcgcgaat tcgcggccgg cctcggcatg cacgtcgagc ccggctgggg
     4261 cgccggcaag ctgttgctcg agctgttcga gaaaaccgtc gagcccgatc tgcagcagcc
     4321 gaccttcgtc accggctatc cgctcgaggt ctcgccgctg gcgcgctgtc gcgacgcgga
     4381 ccccttcctg accgatcgct tcgagctgtt cgtctgcggc cgcgagctcg ccaacggctt
     4441 ctccgaactc aacgaccccg aggagcaggc gcggcgcttc cgcgcgcagg tcgagcagaa
     4501 ggcggccggc gacgaggagg cgatgttcta cgacgccgac tacgtgcgcg cgctggagta
     4561 cggcatgccg ccggccggcg ggctgggcat cggcgtggac cggctggtaa tgctgttcgc
     4621 cgaccagccg tcgatccgcg acgtgctgct gttcccgcag atgcgacccg aggacacctg
     4681 aaaccggggt cagacaccgg ttacctgggg tccgcggggc tctcggttcc ggtcgaccgc
     4741 ggacgggcgc gtggcctcca gctccgattg tccgcgggcg cgcggcgcca taggccggcc
     4801 tgcgctcgga caaggaaaaa ctcgctcggc cggcctatga caccccacac ccgctgcgac
     4861 cgcgacgatg gctatgcccc gcctccgcgg taacgccgag catggcgttg gttgcaagcc
     4921 atgccgacca gcgccgcgca ctggtccggc gccttggctg gtggccgcag cgcttcgccg
     4981 cggtcatgga aggatgtcgg ccagccaccg ggctacggcg ccgagccagg cgacggtcga
     5041 cgccgggcac atcgactccg cagcgggggc gggacgtcat gggcgcgctg agggagctt
//
LOCUS       DAOMPG010000177         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6899_length_5091_cov_3.985902, whole genome shotgun sequence.
ACCESSION   DAOMPG010000177 DAOMPG010000000
VERSION     DAOMPG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_5091_cov_3.985902"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLS34_09555"
     CDS             <1..336
                     /locus_tag="PLS34_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRP87753.1"
                     /translation="VPTSELLSVEEFDEDIVEELRSRARDVLITQAIVKEEKLDQAEP
                     AEDLLSLEGMDKGLAYALASRGIQTREDLADQATDDLEDIEGLDPERAAKLIMAARAH
                     WFESAEENG"
     gene            356..2896
                     /gene="infB"
                     /locus_tag="PLS34_09560"
     CDS             356..2896
                     /gene="infB"
                     /locus_tag="PLS34_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRP87754.1"
                     /translation="MADVTVAQFADVLKVPVDKLLTQLEEAGIRVKGAEDTISDEAKL
                     ELLTFLRESHGREAPAVTPRKITLKRKTQSELRQPVGQGRARTVNVEVRKKRTYIKRD
                     VLEEQARKEQEELDRQRQAEEEAKRVAEEAARRSVEQEQKARDDEEKRRQADEEARLK
                     AEEDARRKAEEEARRIAEEEARVLAEARKRSSDEDRKRREADRAAPRRGGEAPGGTKY
                     GRKELHVAGDKSGKRRKRPTSGRRGAGAQIERQGFERPTAPVVREVAIPETITVAELA
                     QKMAVKATEVIKTMMGMGAMVTINQVIDRDTAVIVVEEMGHTPKILRENQIEEVVEEA
                     VRSSGEEQARWPVVTIMGHVDHGKTSLLDYIRRSKVAAGEAGGITQHIGAYVVESPKG
                     RITFLDTPGHAAFTAMRARGAQATDVVILVVAADDGVMPQTVEAVQHARAAGVPIVVA
                     VNKIDKHGADPERVKSELAQHEVIPEEWGGDTQFVSVSAHTGEGVDELLESVALQAEV
                     LELKAVAAGPAKGVVIESSLEKGRGAVATVLVQQGTLRPGDAVLAGREYGRVRAMFNE
                     LGKPLKEAGPSTPVQVLGLSGTPMAGDDVVVLPDERKAREVALHRQGKFRDVKLAKQQ
                     AAKLEDVFSQMEEGEASAVQLLIKADVQGSAEALRDAMTRLSSDEVKVKVISSNVGGI
                     TESDVNLAAASNAIIIGFNVRADATARNAIKETGVDVRYYSIIYEAIDDVKSAIVGLL
                     SPEIREQIVGIAEVREVFTSPKFGQVAGCLVTEGTVKRNNPIRVLRDNVVIFEGQLES
                     LRRFKDDVNEVRSGTECGIGVRNYNDVRAGDQIECFERVEIARTFQQS"
     gene            2896..3273
                     /gene="rbfA"
                     /locus_tag="PLS34_09565"
     CDS             2896..3273
                     /gene="rbfA"
                     /locus_tag="PLS34_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRP87755.1"
                     /translation="MPREFTRSRRVGEQMQRLLGEILLRDVHDPGAHGVSVTAVEVSR
                     DLSHATVWFSLLDPAADPAPAAAALARAAGMIRGKLGRAMYIRQVPELHFRHDESLER
                     GARLSDLINRAVASDKERQDQDE"
     gene            3266..4195
                     /gene="truB"
                     /locus_tag="PLS34_09570"
     CDS             3266..4195
                     /gene="truB"
                     /locus_tag="PLS34_09570"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRP87756.1"
                     /translation="MNEPVAGGRRPGAGGLDGVVLLDKPLGLTSNVALQKVKRLFGIR
                     KAGHTGSLDPLATGLLPVCLGQATRFSGYLLESAKGYRVTGRLGARTATGDAEGEVVE
                     TCEWRQLDRAAFEAGLAAFQGKLEQVPPMYSALKQGGERLYRKARRGEEVARPPRRIE
                     IRSLALTSWAPPDFSLQLHCSKGTYVRTLVEDVARRAGSCAHVTALRRFAVGPFDEAD
                     MRTLAELEVLAGEGRLAEAVLPCDAALAGLPALQLSPDEAARIRQGQPVEAPGTDDNA
                     LLRLYGGEEFLGVGMRGADGTLRAKRLMATAMA"
     gene            4315..4584
                     /gene="rpsO"
                     /locus_tag="PLS34_09575"
     CDS             4315..4584
                     /gene="rpsO"
                     /locus_tag="PLS34_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRP87757.1"
                     /translation="MPLSVEQKQDIIKEYGRGNADTGSPEVQVALLSARISELTEHFG
                     THKKDHHSRRGLLKLVNQRRQLLDYLKRKDVDRYQDLITRLGLRR"
     gene            4640..>5091
                     /locus_tag="PLS34_09580"
     CDS             4640..>5091
                     /locus_tag="PLS34_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRP87758.1"
                     /translation="MTPTKKSFQYGAHTVTLETGEIARQADAAVVVTMADTVVLVTVV
                     GRKAAAEGQSFLPLTVNYQEKTYAAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLF
                     PKGFLNEVQVIATVMSANPDIDPDIPSLIGTSAALALSGIPFNGPIGAA"
BASE COUNT          929 a         1643 c         1824 g          695 t
ORIGIN      
        1 gtgccgacct cggagctgct gtcggtcgag gagttcgacg aagacatcgt cgaggagttg
       61 cgcagccgcg cgcgcgacgt gctgatcacc caggcgatcg tcaaggaaga aaagctcgac
      121 caggccgagc cggccgagga cctgctgtcg ctcgagggca tggacaaggg tcttgcctac
      181 gcgctggcga gccgcgggat ccagacccgc gaggacctgg ccgaccaggc gaccgacgat
      241 ctcgaggaca tcgagggcct cgaccccgag cgcgccgcga agctgatcat ggcggcccgt
      301 gcgcactggt tcgagagcgc cgaagagaac ggctgaacac gaggttcgat tgcatatggc
      361 cgacgtcact gttgcccagt tcgctgatgt cctgaaggtc ccggtggaca agctgctcac
      421 gcagctcgag gaagcgggta tccgcgtcaa gggcgcggag gacaccatca gcgacgaggc
      481 gaaactcgag ttgctgacct tcctgcggga gagccacggc cgcgaggcgc cggccgtgac
      541 gccgcgcaag atcacgctca agcgcaagac ccagagcgag ctgcgccagc cggtgggcca
      601 gggccgcgcg cgcaccgtca atgtcgaagt gcgtaagaag cgcacctaca tcaagcgcga
      661 cgtgctcgag gagcaggccc gcaaggagca ggaagagctc gaccgccagc ggcaggccga
      721 ggaagaggcc aagcgggtgg cggaggaagc ggcgcgtcgc agcgtcgagc aggaacagaa
      781 ggcccgcgac gacgaggaga agcgccgcca ggccgacgaa gaggcccgcc tcaaggccga
      841 ggaagacgcc cgtcgcaagg ccgaggaaga ggcgcgtcgc atcgcggagg aagaagcgcg
      901 tgtgctggcc gaggcgcgca agcgcagttc ggacgaagac cgcaagcgcc gggaagctga
      961 ccgcgccgcg ccgcgtcgcg gcggcgaggc cccgggcggc accaagtacg gccgcaagga
     1021 gctgcacgtc gccggcgaca agtcgggcaa gcgacgcaag cggccgacca gtggccgccg
     1081 cggcgccggc gcgcagatcg agcgccaggg cttcgaacgc ccgaccgccc cggtcgtgcg
     1141 cgaggtcgcg atccccgaga ccatcacggt cgccgagctg gcgcagaaga tggccgtcaa
     1201 ggccaccgag gtcatcaaga ccatgatggg catgggcgcc atggtcacga tcaaccaggt
     1261 catcgaccgc gacacggcgg tgatcgtggt ggaagaaatg ggccacacgc ccaagatcct
     1321 gcgcgaaaac cagatcgagg aggtggtcga ggaggcggtc cgcagcagcg gcgaggagca
     1381 ggcccgctgg ccggtggtca ccatcatggg ccacgtcgat cacggcaaga cctcgctgct
     1441 cgactacatc cgccgcagca aggtcgcggc cggcgaggcc ggcggcatca cgcagcacat
     1501 cggtgcctac gtggtggagt cgccgaaggg ccgcatcact ttcctggata cgccgggcca
     1561 cgccgccttc accgcgatgc gggcgcgtgg cgcacaggcg acggacgtgg tcatcctggt
     1621 ggtcgcggcc gacgacggcg tcatgccgca gaccgtcgag gccgtgcagc acgcgcgcgc
     1681 ggccggggtg ccgatcgtcg tcgccgtcaa caagatcgac aagcacggcg cggacccgga
     1741 gcgggtcaag agcgagctcg cccagcacga ggtgattccg gaggaatggg gcggcgacac
     1801 ccagttcgtg agcgtttcgg cgcacaccgg tgagggcgtc gacgagttgc tggagtccgt
     1861 ggccctgcag gctgaggtgc tcgaattgaa ggcggttgcc gccggtccgg ccaagggcgt
     1921 ggtcatcgag tccagcctgg agaagggccg cggcgccgtg gcgaccgtgc tggtccagca
     1981 gggcaccctg aggcccgggg acgccgttct ggcggggcgc gagtacggcc gcgtccgcgc
     2041 catgttcaat gagctcggca agccgctcaa ggaagccggg ccgtccaccc cggtgcaggt
     2101 gctcggcctg tccggcacgc cgatggccgg cgacgacgta gtcgtgttgc cggacgagcg
     2161 caaggcacgc gaggtggccc tgcaccgcca gggcaagttc cgcgacgtga agctggccaa
     2221 gcagcaggcc gcgaagctcg aggacgtctt ctcgcagatg gaggagggcg aggccagcgc
     2281 agtgcagctg ctcatcaagg ccgacgtgca gggcagcgcg gaggccctgc gtgacgcaat
     2341 gacccggctg tcctctgacg aggtcaaggt caaggtcatc agcagcaacg tcggcggcat
     2401 caccgagtcg gacgtgaacc tcgcggcagc gtccaacgcc atcatcatcg gcttcaacgt
     2461 ccgcgccgac gccaccgcgc gcaacgccat caaggaaacc ggcgtggatg tgcgctatta
     2521 cagcatcatc tacgaggcca tcgacgacgt gaagtcggcg atcgtcggac tgctgtcgcc
     2581 ggagattcgc gagcagatcg tgggcatcgc cgaggtgcgc gaggtgttca cctcgcccaa
     2641 gttcggccag gtggccggct gcctcgtcac cgagggcacc gtcaagcgca acaacccgat
     2701 tcgcgtgttg cgtgacaacg tggtgatctt cgagggccag ctggagtcgc tgcgacgctt
     2761 caaggacgac gtcaacgaag tgcgctccgg caccgagtgc ggcatcggcg tgcgcaacta
     2821 caacgacgtc cgtgcgggcg accagatcga gtgcttcgag cgggtcgaga tcgcgcgtac
     2881 cttccagcaa agctgatgcc cagggagttc acgcgcagtc gccgggtcgg cgagcagatg
     2941 cagagactgc tgggcgagat cctgctgcgc gacgtgcacg acccgggcgc gcacggcgtc
     3001 agcgtgacgg ccgtcgaggt gtcgcgcgac ctgtcgcacg cgacggtctg gttcagcctg
     3061 ctcgaccccg ccgcggatcc ggcgccggca gctgcggcgc tggcccgggc tgccggcatg
     3121 attcgcggca agctgggacg tgcgatgtac attcgccagg tgcccgaatt gcatttccgg
     3181 cacgacgaga gcctggagcg cggcgcccgc ctatccgacc tgatcaaccg ggcggttgcg
     3241 tccgacaagg aaaggcaaga ccaggatgaa tgagccggtc gccgggggcc gccggccggg
     3301 cgccgggggt ctcgacggcg tggtgctgct cgacaagccg ctcggcctta catcgaacgt
     3361 cgccctgcag aaggtcaagc ggctgttcgg gatccgcaag gccggccaca ccgggagcct
     3421 cgacccgctg gcaaccggcc tgctgccggt ctgcctcggc caggccacgc gcttctcggg
     3481 ctacctgctc gagtcggcga agggctaccg cgtgaccggc cggctgggtg cgcgcaccgc
     3541 caccggcgat gccgaaggcg aagtggtcga gacctgcgag tggcggcagc tggatcgggc
     3601 ggccttcgag gccgggctgg cggccttcca gggcaagctc gagcaggtgc cgccgatgta
     3661 ctcggcgctc aagcagggcg gcgagcgcct gtaccgcaag gcacgccgcg gcgaggaagt
     3721 ggcgcgcccg ccgcgccgga tcgagatccg gtcgctggca ctgacgagct gggcgccgcc
     3781 ggatttttcc ctgcagctgc attgttccaa ggggacttac gtgcggacac tggtcgagga
     3841 cgtggcgcgc cgtgccggca gctgcgcgca cgtcacggcc ttgcggcggt tcgccgtcgg
     3901 cccgttcgac gaggccgaca tgcgcacgct ggcggagctc gaggtgttgg cgggagaggg
     3961 tcgcctggcc gaggcggtgc tgccctgcga cgcggcgctc gccgggctcc cggcactgca
     4021 gctcagcccg gatgaggcgg cgcgtatccg gcagggacag ccggtcgaag cgcccggcac
     4081 ggacgacaat gcgctgctaa ggctttacgg cggcgaggaa ttccttggcg tcggcatgcg
     4141 cggagcggac ggcaccctgc gggcgaaacg cctcatggcg accgcgatgg cgtagctaag
     4201 acgtcgaagt taaaggtttt ttagtggcgg caggcttgtt cgatcgcggc tccacgcgat
     4261 acaattgcgg gcttgtcaac gggacagcaa agagaaggtt tcaggagata tctcatgcct
     4321 ctgtcggtcg agcagaaaca ggacatcatc aaggagtacg gccgcggcaa tgccgacacg
     4381 ggctccccgg aagtgcaggt ggcgttgttg tccgcccgca tcagcgagct cactgagcat
     4441 ttcggtacgc acaagaagga ccaccattcc aggcgtggcc tgctgaagct ggtcaaccag
     4501 cgccggcagc tcctcgatta cctgaagcgg aaggacgtcg accgctacca ggacctcatt
     4561 acccgtctcg gcctgcgtcg ctgagtatct tccaagtccg gtcatgggga ccggcagaaa
     4621 acaggcgaag aaagtgaatc tgactcctac caagaaatcc ttccagtacg gcgcccacac
     4681 cgtcacactc gaaaccggcg agattgctcg ccaggccgac gcggcggtcg tggtcaccat
     4741 ggccgacacc gtggtgctgg tcaccgtcgt gggccgcaag gcggcggccg agggccagag
     4801 tttcctgccg ctgacggtca actaccagga aaagacctac gccgcgggcc gcatccccgg
     4861 cggcttcttc aagcgcgagg gtcggcccac cgagaaagag acgctgacct cgcggctgat
     4921 cgaccggccc atccggccgc tgttcccgaa gggcttcctg aacgaagtcc aggtgatcgc
     4981 cacggtgatg tcggcgaacc cggatatcga cccggacatt ccgtcgctca tcggtacctc
     5041 cgcggcgctg gctttgtccg gcattccgtt caacggcccg atcggcgctg c
//
LOCUS       DAOMPG010000178         5072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6937_length_5072_cov_4.483556, whole genome shotgun sequence.
ACCESSION   DAOMPG010000178 DAOMPG010000000
VERSION     DAOMPG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6937_length_5072_cov_4.483556"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLS34_09585"
     CDS             <1..973
                     /locus_tag="PLS34_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRP87759.1"
                     /translation="AGQLSFAQVRAVIRVATPDNEATLLMWAQHSTASQMETFARLYR
                     RHGENAAVMRQHRERSFTSWEEDDGMVSFRIRLPAEQAAVVLQAVEAAKASLEGRDRE
                     DDVSAETPPSPWDDPPAHPDEDVSAETRRARKADALVRVAESYLEPDGRPRSLAEKYQ
                     VHVHLDLRRDTDGPVIDPDAPAIRRALRLRDRGCRFPGCTHTRHVDAHHVRHWADGGE
                     TKLSNLVELCRGHHRLLHEGGYGLRVTDDGALVFTRPDGERIPDVPRPLVLTGDPVAV
                     LAADNHRHGVSAETFRCQWDGKPPDYGAMVEILDWKRPPPAEGHAHE"
     gene            1047..1122
                     /locus_tag="PLS34_09590"
     tRNA            1047..1122
                     /locus_tag="PLS34_09590"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1080..1082,aa:Ala,seq:ggc)
     gene            1154..1229
                     /locus_tag="PLS34_09595"
     tRNA            1154..1229
                     /locus_tag="PLS34_09595"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1188..1190,aa:Glu,seq:ttc)
     gene            complement(1303..1737)
                     /locus_tag="PLS34_09600"
     CDS             complement(1303..1737)
                     /locus_tag="PLS34_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87760.1"
                     /translation="MRSTELDNLVRIGRLKPEPPSERELAGLLASARVRLEDAGQASL
                     SFDSRFDLAYNASHSLALYALRRLGYRSDHRYLAFQALPHSTGLPPEVWRVLAKAHQQ
                     RNRAEYEGHLERDERLLADLITAAKRLSDVVSALPPVDEQNA"
     gene            complement(1724..2386)
                     /locus_tag="PLS34_09605"
     CDS             complement(1724..2386)
                     /locus_tag="PLS34_09605"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRP87761.1"
                     /translation="MPFVPFLGMVIPVMGTNVEKLAQPSAREALAAALFTPVQQRVLG
                     LLFGQPERRYQSAELIRLAEAGTGAVHRLITRLAATGLVSVASVGNQKFYQANPESPV
                     FAELCGLIRKTVGLAGPLQEALAPLADRITAAFVYGSIAKGIDQASSDIDLMVITDDL
                     DYAALYAALTGAEAVLSRPINPNVMSAADWRLKRTQKDSFVARIADQPKVFVIGSEHA
                     ID"
     gene            complement(2473..3015)
                     /locus_tag="PLS34_09610"
     CDS             complement(2473..3015)
                     /locus_tag="PLS34_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87762.1"
                     /translation="MRASRFSGGPAWLQALTLAAALALGLAPEAAPTGAEAPLPEDEA
                     RMLLQRYVASWDLDALLAEPPVAREFQALPAAAREQLLRNLSVRGTIDVYGGALSIMG
                     NAPHRGGEDEALLCVEVLGGAVRLHAAILSGGRVTVHTRGEQYSYLPICVRDWVAVVS
                     TGHHYRFRPPANLELVRLPE"
     gene            complement(3026..3481)
                     /locus_tag="PLS34_09615"
     CDS             complement(3026..3481)
                     /locus_tag="PLS34_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87763.1"
                     /translation="MTETLSGTVSMLQFSSETTGQVSGSYGRVQGSTHTKQVATFRVD
                     GRPAQIKLLGQPSLSDGDVVLLAGAMKNGTFHARAMRNESSGAIDHRPAVPSLVIGVL
                     MVAIGLPLSLIIIGLPFLAFGIYNIWDGFKTKKAISMVRGASPAVLAPG"
     gene            3726..>5072
                     /locus_tag="PLS34_09620"
     CDS             3726..>5072
                     /locus_tag="PLS34_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRP87764.1"
                     /translation="MDRSRPLYRWRFGSAEFDEARHELRLGGLPVELESRPLEVLAVL
                     LRHAGEVVTKRELLDEVWRDRVTVEHVLATAVGKLRQALGPEGPQLIVTVPRIGYRLQ
                     AEVQRTAVGQAHVSELALAPGAPVPGREHFLLERPLGASHASEVWLARHPGSGARRVF
                     KFATSVARLDLLKREATVARVLVAGLGERPDIARLLDWNFETAPFFLECEYGGQSLGA
                     WAADGKLQALPATRRLALFLAVAGAVDAAHSVGVLHKDIKPDNVLIDESGAVRLTDFG
                     SSQLLDPGRLAALGITALGMTVDSSTAGLSGTLLYLAPELLDGQPATVRSDVYALGVL
                     LYQLKVGDLHRPLTPGWERAVGDPELRADISRATDMVPGRRFGSVAEFMERLSRLEER
                     RRERLAAQVEAENAAVARAALLRMRARRPWIAAAMLSLSVGLLAALWLHREAQIARA"
BASE COUNT          773 a         1762 c         1761 g          776 t
ORIGIN      
        1 cgccggccag ctgtccttcg cccaggtgcg cgccgtgatc cgcgtcgcga cgccggacaa
       61 cgaggcgacc ctgctgatgt gggcgcagca ctccacggcc tcgcagatgg agaccttcgc
      121 ccggctgtat cgccgccatg gcgagaacgc ggcggtcatg cgccagcacc gcgagcgctc
      181 cttcacttcc tgggaagaag acgatggcat ggtgtcgttc cgcatccgcc tgccggccga
      241 gcaggcggcg gtggtgctgc aggccgtcga ggcggcgaag gcctcgctgg aagggcgcga
      301 ccgcgaagac gacgtctccg cggagacgcc gccctcgccc tgggacgatc cgcccgccca
      361 tcccgacgaa gacgtctccg cggagacgcg gcgggcgcgc aaggccgacg cgctggtgcg
      421 ggtggcggaa tcctacctgg agccggacgg ccggccgcgc tcactggccg agaagtacca
      481 ggtgcacgtg cacctcgatc tcaggcgcga cacggacggg ccggtgatcg atccggacgc
      541 gccggcgatc cgccgggccc tgcggctgcg tgaccgcggc tgccgcttcc cgggctgcac
      601 gcacacccgc catgtcgacg cgcaccacgt gcgccactgg gccgacggcg gcgagacgaa
      661 gctctccaac ctggtggagc tgtgccgcgg ccatcaccgg ctgctgcacg agggcggtta
      721 cgggctgcgc gtcaccgacg acggtgcgct ggtgttcacg cgcccggacg gcgagcgcat
      781 ccccgacgtg ccgcggccgc tggtgctgac cggcgacccg gtggcagtgc tcgccgcgga
      841 caatcatcgc cacggcgtct ccgcggagac gtttcgctgc cagtgggacg gcaagccgcc
      901 ggattacggg gcgatggtgg agatactcga ctggaagcgg ccgccgcctg ccgaggggca
      961 tgcgcacgaa tgaccggcgc ctggatccgg ccaccaggca gtcttgccgc ggcaagggcg
     1021 gttgtttatc atgcccgact tcgtgtgggg ccatagctca gctgggagag cgcttgcatg
     1081 gcatgcaaga ggtcggcggt tcgatcccgc ctggctccac cagaatccac tctcagcgct
     1141 cagtccgcac tgcgtcccct tcgtctagag gcctaggaca ccgccctttc acggcggcga
     1201 caggggttcg aatcccctag gggacgccac tttcccactg tcgaatcgac gagcctggtc
     1261 gccttctgtc ctggcatttg gcgctggggt caagctccag gctcaggcgt tttgctcgtc
     1321 aacaggcggc aaggcgctaa ccacgtcgct cagccgtttg gctgcggtga tcaagtcggc
     1381 gagcaagcgc tcatcgcgct ccagatgtcc ctcgtattcc gcgcgattgc gctgttgatg
     1441 cgctttggcc agaacccgcc acacttcggg tggcaagccg gtcgaatgcg gcagggcctg
     1501 gaacgccaga taacgatggt cgctgcggta gcccaggcgg cgcagggcat acagcgccaa
     1561 cgagtggctt gcgttgtaag ccaggtcgaa gcggctgtcg aaactcagtg aggcctggcc
     1621 tgcgtcttcg agccgtacgc gggcggaggc caacaggccc gctagctcgc gttcggatgg
     1681 tggctctggc ttcagcctgc cgatccggac caggttgtcc agttcagtcg atcgcatgct
     1741 cggatccgat cacgaagacc ttgggctggt cggcgatgcg tgcgacaaag ctgtccttct
     1801 gagtgcgctt gaggcgccag tctgcggcgc tcatcacgtt cggattgatc ggccgagaca
     1861 gcacagcctc ggcaccggtc aacgccgcgt acaaggccgc gtagtccagg tcatcggtga
     1921 tcaccatcag atcgatgtcg ctcgaggctt gatcgatgcc cttggcaatc gaaccataaa
     1981 cgaatgctgc ggtaattcga tcggccagcg gcgccagcgc ctcctgcagc gggccggcaa
     2041 ggcccaccgt cttgcgaatc agcccgcaca gctccgcgaa caccggcgat tcaggattgg
     2101 cctggtagaa cttctggttt cccacagaag caaccgaaac gagcccggtc gcggccagcc
     2161 tggtaatcag gcgatgcacc gcgccggtgc ccgcctcggc cagccggatc aactcggcgc
     2221 tctgatatcg ccgttcgggc tggccgaaca gcaaacccag cacgcgctgc tgcacgggcg
     2281 taaacagggc agctgcaagc gcttcgcggg cagacggctg ggcaagcttt tcgacattcg
     2341 ttcccataac gggaatgacc atacccaaaa agggaacgaa tggcaatggc caatcggggc
     2401 cgtgcctgat cgatgaatcg ggccgtccct tcgagcgtcg cgggagacgc cgccttcacg
     2461 cctggcgacc gatcactccg gcaggcgcac caactccagg ttggccggcg gccgaaaccg
     2521 gtaatggtgc ccggtgctga ccaccgccac ccagtcgcgc acgcagatcg gcaggtaact
     2581 gtattgctcc ccgcgcgtat gcacggtcac acggccgccc gacaggatgg ctgcgtgcag
     2641 ccggacagca ccgccaagga cctcgacgca caacagcgcc tcgtcctcgc cgccccggtg
     2701 cggcgcgttg cccatgatcg acagcgcccc cccatagacg tcgatggtgc cgcgcaccga
     2761 caggttgcgc agcagctgtt cgcgtgccgc cgccggcagg gcctgaaact cgcgcgccac
     2821 cggcggctcg gccagcaggg catcgagatc ccagctggcc acgtagcgct gcagcagcat
     2881 gcgtgcctcg tcctcgggca acggtgcctc cgcgccggtc ggcgccgcct cgggcgccag
     2941 tcccagcgcc aacgccgccg caagcgtcaa tgcctgcagc caggccgggc cgccggagaa
     3001 gcgtgacgcc cgcaaaggcc ccgcctcagc cgggcgccag cacggccggg ctggcgccac
     3061 gcaccatcga gatggctttc ttggtcttga agccgtccca gatgttgtag atgccgaagg
     3121 cgaggaacgg cagcccgatg atgatgagcg acaacggcag tccgatcgcc accatcagga
     3181 cgccgatcac caggctcggc accgcgggtc gatggtcgat ggccccggag ctctcgttgc
     3241 gcatggcgcg cgcatggaag gtgccgttct tcattgcgcc ggccagcagt acgacgtcgc
     3301 cgtcgctgag gctcggctgg ccgagcagct tgatctgcgc cggccggccg tcgacgcgga
     3361 aggtggccac ctgcttggtg tgggtgctgc cctgcacgcg gccgtaggag ccgctgacct
     3421 ggccggtggt ctccgacgag aactgcagca tgctgacggt gcctgaaagc gtctctgtca
     3481 tgactcttct ccccctgtgg tggtgccgcc gcaatgtcgc gcgcccgcaa cgcccgacct
     3541 gcgacttgta atggcctgga tctgccgccc gaatctgccg gcgccgtcct catctgtcct
     3601 cacgccgcca tcaagaaagg ttcaagccgc cgccgatccg cgcggcgcgg ctatggcgca
     3661 tcgacccggc ccgggagtag catcgcgcca cagcctgtcg gctgcctcct gcccgcggag
     3721 ccgccatgga ccgttcgcgt cccctgtatc gctggcgctt cggctcggcc gagttcgacg
     3781 aagcgcgcca cgagctgcgc ctcggcgggc tgccggtgga actggagtcg aggccgctcg
     3841 aggtgctggc ggtattgctg cgccatgccg gcgaggtggt caccaaacga gagttgctcg
     3901 acgaggtctg gcgcgaccgc gtgacggtcg agcacgtgct ggcgaccgcg gtgggcaagc
     3961 tgcgccaggc gctcggtccc gaaggcccgc agctgatcgt caccgtgccg cgcatcggtt
     4021 accggctgca ggcggaggtg cagcgcaccg cggtcggcca ggcgcacgtc agcgaactgg
     4081 cgctggcgcc cggcgcgcca gtgccgggcc gcgagcactt cctgctggag cgcccgctcg
     4141 gcgcctcgca tgcgagcgag gtctggctgg cccggcatcc gggcagcggc gcgcggcgcg
     4201 tcttcaagtt cgccaccagc gtggcgcgcc tcgacctgct caagcgcgaa gccacggtcg
     4261 cgcgcgtgct ggtggccgga ctcggcgagc gccccgacat cgcgcggttg ctggactgga
     4321 atttcgagac cgcgcccttt ttcctcgagt gcgagtacgg cggccagagc ctcggcgcct
     4381 gggcggccga cggcaagctc caggcgctgc ccgccacgcg ccggctggcg ctgttcctgg
     4441 cggtggccgg cgcagtcgac gcggcgcaca gcgtcggcgt gctgcacaag gacatcaagc
     4501 cggacaacgt gctgatcgac gagagcggcg cagtgcgcct gacggatttc ggcagcagcc
     4561 agctgctcga tccgggccgg ctggcggcgc tgggcatcac ggcgctgggc atgaccgtcg
     4621 attcctcgac cgccggcttg tccgggacgc tgctgtatct cgcgccggag ctgctggacg
     4681 gccagccggc gacggtgcgc agcgacgtct acgccctcgg cgtgctgctc taccagctga
     4741 aggtcggcga cctgcaccgg ccgctgacgc ccggctggga gcgcgcggtc ggcgacccgg
     4801 agttgcgcgc agacatcagt cgcgccaccg acatggtgcc cgggcggcgc ttcggctcgg
     4861 tggccgagtt catggagcgc ctgtcgcgcc tggaggagcg gcgccgcgag cggctggcgg
     4921 cgcaggtcga ggccgagaac gcggcggtcg cgcgggccgc gttgctgcgc atgcgggcgc
     4981 gtcgcccctg gatagcggcc gcgatgctgt cgctgtcagt cgggctgctc gcggcgctgt
     5041 ggctgcaccg cgaggcgcag atcgcgcgcg cc
//
LOCUS       DAOMPG010000179         5047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_6973_length_5047_cov_4.056090, whole genome shotgun sequence.
ACCESSION   DAOMPG010000179 DAOMPG010000000
VERSION     DAOMPG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_5047_cov_4.056090"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="lptD"
                     /locus_tag="PLS34_09625"
     CDS             <1..477
                     /gene="lptD"
                     /locus_tag="PLS34_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRP87765.1"
                     /translation="PNLAASSPIVLELGLGLFNNWNADLIYQWDNNADQTLLAQYRVQ
                     YQPAPNKVVNLSYRYRPELLEDVAFSFGWPLARRWSLVSALEYSLRDNTTVDQLVGLQ
                     YETCCWALRFSASEQVSGRDGSTDTALRLQVEFKGLGGTGARQRFESDILGYSVYD"
     gene            488..1810
                     /locus_tag="PLS34_09630"
     CDS             488..1810
                     /locus_tag="PLS34_09630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP87766.1"
                     /translation="MRLIYKYFSLFVLLLAGTLPGTRGALHAEPLSESGVLLDRIVAL
                     VDDGVVLQSELDRQVELVTHQLRSQGTRMPPEAVLRQQILESLIVQQVQMQRAERIGI
                     RVGDQQLNAALQQVAQRNGTTLSQLPDRLAADGVDYTAFREQLRREMTIDGLRQRDVM
                     ARIQVSDREVRRWLEQQEANRGNLVDFDISQILIALPEDPTPEQRDAAERRAAEIHAR
                     LVGGADFAAIAVAESGGQQALSGGRLGWRRGSQLPQQFAAVIQSLAAGEISQPVRSSS
                     GFHIFRVNETRGGEEKIVQLQTRARHILITPNEILDDETVRGRLEAIRERVAAGESFA
                     DIAMLESGDASSAARGGDLGWNPPGTFVPEFDAALERLSPGEISEPIRTMFGWHIILL
                     EDRQERDTTTEVRRMQAVQAIRASKLEQETELWLRRLRDEAWVEILGG"
     gene            1826..2812
                     /gene="pdxA"
                     /locus_tag="PLS34_09635"
     CDS             1826..2812
                     /gene="pdxA"
                     /locus_tag="PLS34_09635"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRP87767.1"
                     /translation="MTASPAIALTTGEPAGIGPELCLAVAARPWPARLVAVGPLAHLQ
                     DTASRLNLAVALKPVSHAGPHRAGELQVIDVPLAVPATPGRPDPRNADAVLAMLRRAA
                     QGCLAGEFDGMVTAPVHKAVINDAGHPFSGHTEFLAELSGGRPVMMLLAGSLRVALAT
                     THLPLSRVPAAIERDSLTAVLRILHGELRNRFGIAVPRILVAGLNPHAGEGGHLGDEE
                     IRVIGPVLDTLRGEGLLLEGPLPADTLFTPDRLARADAVLAMYHDQGLPVLKYVGFGR
                     AVNITLGLPLLRTSVDHGTALDIAGTGRADPGSLVAALELAVEIAGRHSAPR"
     gene            2809..3612
                     /gene="rsmA"
                     /locus_tag="PLS34_09640"
     CDS             2809..3612
                     /gene="rsmA"
                     /locus_tag="PLS34_09640"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRP87768.1"
                     /translation="MSHQPRKRFGQHFLHDPRVIERIIEAVNPAPGDQLVEIGPGEGA
                     LTLPLLARGARLTVIELDRDLAARLAARPEAGATLTVVQGDALKVSLARFAAPGERLR
                     LVGNLPYNVSTPLLFRFIEQAGVIRDMHFMLQREVVERMAAPPGSKTYGRLSVMLAAA
                     CRVEALFEIGPGAFRPPPKVWSSVVRLEPWTAPPFPVPDAKLFAEVVRRAFGQRRKTL
                     RNALARLLDEADIRAADCDPGARAETLPAAAFGRLATVAARNWGQSPIS"
     gene            3685..4116
                     /locus_tag="PLS34_09645"
     CDS             3685..4116
                     /locus_tag="PLS34_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRP87769.1"
                     /translation="MDYQRIIAAVDLDEHSDLVLSRAKAVAGVTGAQLRLMHVVEYVP
                     VEPMGEALLPAVNIEEELVAGARTRLAELAAAAGLDFAEQRVIAGTIKSELLREAREW
                     PADLVVLGSRERHGLALIVNLTEDAVLHAAPCDVLAVRLSG"
     gene            4174..4548
                     /gene="apaG"
                     /locus_tag="PLS34_09650"
     CDS             4174..4548
                     /gene="apaG"
                     /locus_tag="PLS34_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRP87770.1"
                     /translation="MTDPSIRIEVETAYLDHQSDPGEGRYAFAYSITISNDGPIAARL
                     LSRHWIITDADGGVQEVRGEGVVGEQPLIRPGEGFRYCSGAIIPTPVGSMRGAYHMVD
                     ETGHRFEAQIAPFTLARPGALH"
     gene            4548..>5047
                     /locus_tag="PLS34_09655"
     CDS             4548..>5047
                     /locus_tag="PLS34_09655"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRP87771.1"
                     /translation="MAVYAIGDLQGCLDPLQRLLDRIAFDPARDRLWFTGDLVNRGPA
                     SLETLRFVRSLGELAVVVLGNHDLHLLVAACTGNRKTRRNDTLGEILVAPDRDELLHW
                     LRHRPLLHHDPAVDFTMVHAGLPPQWDLADAVAAAAELRSVLAGDDFVAFLEEMYGNL
                     PNRWSP"
BASE COUNT          762 a         1770 c         1720 g          795 t
ORIGIN      
        1 cccaaccttg ccgcaagctc tcccatcgtc ctcgagctcg ggctcgggtt gttcaacaac
       61 tggaatgccg atctgatcta ccagtgggac aacaacgccg atcagacact gctggcgcaa
      121 taccgggtcc agtaccagcc ggcgcccaac aaggtagtga acctttccta ccgctatcgg
      181 cctgaattgt tggaggacgt ggcgttttcc ttcggctggc cgctggcgcg gcgctggagc
      241 ctcgtcagcg ccctggagta ctcgctgcgc gacaacacga ccgtggacca gctcgtcggc
      301 ctccagtacg agacctgctg ctgggcgctg cgcttctcgg cttcggagca ggtcagcggc
      361 cgcgacggca gcacggacac ggccctgcgg ctgcaggtgg aattcaaggg tctcggcggc
      421 accggcgcga ggcaacgctt cgagagtgat atccttggct attccgttta cgactgaata
      481 aacatccatg cgactgattt ataaatattt ttccctgttc gtactgctgc ttgccggtac
      541 ccttccgggc acgcgcggcg cgctgcatgc cgagccgctg agcgaatcgg gggtgctgct
      601 ggaccgaatc gtagcgctgg tcgacgacgg cgtggtcctg cagtccgaac tcgatcgcca
      661 ggtcgaactg gtgacgcacc agttgcgcag ccagggcact cgcatgccgc cggaggcggt
      721 cctgcggcag cagattctcg agagcctcat cgtccaacag gtacagatgc agcgggccga
      781 gcggatcggt atccgcgtcg gcgaccagca gctgaacgcc gccctgcaac aggttgcgca
      841 gcgcaatggg accaccttgt cccagttgcc agaccggctg gcggccgacg gcgtggacta
      901 cacggccttt cgcgagcaac tgcgccgcga aatgaccatc gacggcctgc gccagcgcga
      961 cgtcatggcg cgaatccagg tatcggaccg cgaggtccgg cgctggctcg agcagcagga
     1021 ggccaaccgc ggcaacctgg tcgactttga catctcccag attctcatcg cgctgccgga
     1081 agaccccacg ccggagcagc gcgatgcggc agaacggcgc gccgcggaga ttcatgcgcg
     1141 actggtgggc ggcgctgatt ttgccgcgat cgcggtcgcc gaatcgggtg gccagcaggc
     1201 actgagcggc ggtcgcctcg gctggcggcg gggctcgcag ctgccgcagc agtttgcggc
     1261 cgtgatccag tcgctcgccg ccggcgagat cagccagccg gttcgcagca gcagcggttt
     1321 tcacattttc cgcgtcaacg agacccgcgg cggcgaggaa aagatcgtgc agctgcagac
     1381 acgcgcgcgg cacatcctca tcacgcccaa tgaaattctc gacgacgaga ccgtgcgcgg
     1441 ccgtctcgag gcgatccgcg agcgcgtcgc cgccggtgag tccttcgccg acatcgccat
     1501 gctcgagtcc ggcgacgcct cctcggcagc gcgcggcggc gacctgggct ggaacccccc
     1561 cggcaccttc gtaccggaat tcgacgccgc cctggagcgc ctgagtcccg gcgagatcag
     1621 cgagccgatc cgcacgatgt tcggctggca catcatcctg ctggaggacc gccaggagcg
     1681 cgacacgact acggaagtca ggcgcatgca ggcggtgcag gccatccggg ccagcaagct
     1741 ggagcaggaa accgagctct ggctgcggcg gctgcgggat gaggcctggg tcgagatcct
     1801 cggcggctga gccccgccgc gcccgatgac cgcgtcgccg gccattgcat tgaccaccgg
     1861 cgagcccgcg ggcatcggcc ccgagctctg cctggcggtc gccgcccgac cgtggccggc
     1921 gcgcctggtg gcagtcggcc ccctggcgca tctgcaggac acggcctcgc gcctgaacct
     1981 ggccgtggcg ctgaagcccg tcagccatgc cgggccgcac cgcgccggcg agctgcaggt
     2041 catcgacgtg ccgctggcgg tgcccgcgac cccgggccgt cccgacccgc gcaacgccga
     2101 cgcggtgctc gcgatgctgc gtcgcgcggc acaagggtgc ctggccggcg aattcgacgg
     2161 catggtcacg gcgccagtcc acaaggccgt gatcaacgac gcgggtcacc cgttctccgg
     2221 ccacaccgag tttctcgcgg aactgtccgg cggacgcccg gtgatgatgc tgctggccgg
     2281 gtcgctgcgc gtcgccctgg ccacgacgca cctgcccttg tcgcgcgtgc cggctgcaat
     2341 cgagcgggat tcgttgacgg cggtgctgcg cattctccac ggtgagctca ggaatcgctt
     2401 cggtatcgcc gtgccgcgta tcctcgtcgc cggcctgaac ccgcacgccg gcgagggcgg
     2461 acacctcggc gacgaggaga tccgggtgat cggcccggtg ctggacacgc tgcgcggcga
     2521 gggactgctg ctcgaggggc cgctgccggc cgacaccctg ttcacgcccg accggctggc
     2581 ccgcgccgac gccgtgctgg ccatgtacca cgaccagggc ctgcccgtgc tcaagtacgt
     2641 cgggttcggg cgcgccgtca acatcacgct ggggctgccg ttgctgcgca cctcggtcga
     2701 ccacggcacg gcgctggaca tcgccggcac ggggcgcgcg gatccgggca gcctcgtcgc
     2761 cgcgctggag ctggccgtcg agatcgccgg ccggcacagc gcgccgcgat gagccaccag
     2821 ccgcgcaagc gcttcggcca gcacttcctg catgatccac gggtcatcga gcgcatcatc
     2881 gaggccgtca acccggcgcc gggcgaccag ctggtggaga tcggcccagg ggaaggcgcg
     2941 ctcaccctgc ccctgctggc gcgcggcgcc cggctgacgg tcattgagct ggatcgcgac
     3001 ctcgcggcgc ggctcgcggc gcggccggag gcgggtgcaa cgctgaccgt cgtgcagggc
     3061 gacgcgctca aggtgtcgct cgcccggttc gccgcgcccg gtgagcgcct gcggctggtg
     3121 ggcaacctgc cctacaacgt ctccaccccc ctgctgttcc gcttcatcga gcaggccggc
     3181 gtgattcgtg acatgcattt catgctccag cgggaggtcg tcgagcgcat ggcggcgcca
     3241 ccgggctcga aaacctacgg ccgcctgagc gtgatgctgg cggcggcctg ccgcgtggaa
     3301 gcgctgttcg agatcggccc gggtgcgttt cgcccgccgc cgaaggtctg gtcgtccgtg
     3361 gtgcggctcg agccgtggac cgcgccccct ttcccggtgc cggatgcgaa gttgttcgcc
     3421 gaggtcgtgc gccgggcctt cggccagcgc cgcaagacgc tgcgcaacgc cctcgcccgc
     3481 ctgctcgatg aggccgacat ccgcgcagcg gactgcgacc cgggcgcgcg cgcggagacg
     3541 ctgcccgccg cagccttcgg acgccttgca acggtcgccg cccggaattg gggacagtcc
     3601 ccgatttcct agcggcaatt ggggagtgtc cccaattccg tcgccatcta tactcagggc
     3661 aggagcatcc aggggagcgc gcatatggac taccagcgga tcattgcagc cgtcgatctc
     3721 gatgagcaca gcgacctggt gctctcccgt gccaaggccg ttgccggcgt gaccggcgcc
     3781 cagctacggc tcatgcacgt cgtggaatac gtgccggtcg agccgatggg cgaggcgttg
     3841 ctgccggcgg tcaacatcga ggaggaactg gtcgccggcg cccgtacgcg gttggcggaa
     3901 ctggccgccg cggccggcct cgacttcgca gagcaacgcg tgatcgccgg caccatcaag
     3961 agcgagctgc ttcgcgaggc gcgcgagtgg ccggccgatc tcgtcgtgct cggcagccgc
     4021 gaacgccacg gtctcgcgct gatcgtcaac ctgaccgagg acgcggtgct gcatgccgcg
     4081 ccgtgcgacg tgctggcggt gcgcctctcc ggataggccg ccgtgctcgt gtagaatctg
     4141 gagtacacga gacgccctgt cacggagcgc cccatgactg atccttcgat ccggatcgag
     4201 gtggaaaccg cgtacctgga tcaccaatcc gatcccggcg agggccgcta cgccttcgcc
     4261 tacagcatca cgatctcgaa tgacggaccg atcgccgccc gacttctttc ccgacactgg
     4321 atcatcaccg acgcggacgg cggtgtgcag gaagtgcgcg gcgagggtgt ggtcggcgaa
     4381 cagccgctga tccgccccgg cgagggattc cgctactgca gcggggccat catccccacg
     4441 ccggtcggct cgatgcgcgg cgcctaccac atggtcgacg agaccgggca ccgcttcgag
     4501 gcacagatcg ccccgttcac gcttgccagg ccgggcgcac ttcactgatg gcggtttacg
     4561 ccatcggtga tctgcagggt tgtctcgatc cgctgcaacg gctgctggat cgcatcgcct
     4621 tcgatcccgc gcgcgaccgg ctatggttca ccggcgacct ggtcaaccgc ggccccgcct
     4681 cgctggagac gctgcgcttc gtgcgctcgc tcggcgagct ggccgtcgtg gtgctgggca
     4741 accatgacct gcacctgctc gtcgccgcct gcacgggcaa ccgcaagacc aggcgcaacg
     4801 acacgctcgg cgagatcctg gtcgcgccgg atcgcgatga gctactgcac tggctgcgcc
     4861 accggccgct gctgcatcac gatccggcgg tggacttcac catggtgcac gcgggcctgc
     4921 cgccgcagtg ggacctggcg gacgccgtcg cggccgccgc tgaactccgg tcggtgctgg
     4981 cgggtgacga cttcgtcgct tttctcgagg agatgtacgg caaccttccg aaccgctggt
     5041 cgccgga
//
LOCUS       DAOMPG010000180         5017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7032_length_5017_cov_3.449416, whole genome shotgun sequence.
ACCESSION   DAOMPG010000180 DAOMPG010000000
VERSION     DAOMPG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7032_length_5017_cov_3.449416"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLS34_09660"
     CDS             complement(<1..300)
                     /locus_tag="PLS34_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87772.1"
                     /translation="MSEPTSLWAELKRRNVVRVAAAYAVIAWLLIEVSDTIFPRLGLP
                     EWTVTLVIALLLLGLPLALFLAWAFELTPDGMKRTEDTAGEAPTPRTGWRRTDRLI"
     gene            complement(297..2159)
                     /locus_tag="PLS34_09665"
     CDS             complement(297..2159)
                     /locus_tag="PLS34_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP87773.1"
                     /translation="MRRRNVFRAGLAWLALAWLLIAIADLLFPAFGFPDAVVRWLIIG
                     LALALLPVMLFAWLFRLTPDGLRLRRGPQGDDPVTARAGRRTDQLTLVLLLLAVGFSA
                     VKQFVLPQRVTPPTQVQAPAPAPVPAPDPRPPAPPGPVDPLSIAVLPFANLSPDPENA
                     YLADGMAEEILNVLARVEGLKVASRTSSFSLRDQRLGSREIGRQLGVAHVLDGSVRRQ
                     GEQLRITAQLVAAATDQQLWSGTFDRQLTDIFSLQEEIAQAITDALADTLGVRKVEVR
                     QATADLQAYELYLRGRQLFAQRGANLAAARELLEQAVARDPGFAEAWATLAGALYVTP
                     SYSSELSGDEAVGRARAAAERALALLPGQPDALAVSGRIAAERGDRVAAAELIERALE
                     SDPNNANTWTWRGLVLIETGHIGAAREAFEHAMRLDPLSGIHVGWLGTAQLFGGEHAV
                     AEANLKRAHALGWRGPASFMRLKLALAGEPGADMARRFEDWLHDDGRIAPEHRATHEA
                     VLPAIREPARREAARAAIDAAVQSWPENEWAQLYLLAGLPDEAINEALRPKPVSGQIL
                     LMLIWSSVDRAFREHPRFMELAARDGLTAFWDRFGEPDFCRRTAGPPPGMECER"
     gene            2314..3831
                     /locus_tag="PLS34_09670"
     CDS             2314..3831
                     /locus_tag="PLS34_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRP87774.1"
                     /translation="MARVHEVLIIGSGFGGSIAAARLAEAGVDVTLLERGPWRDTLPV
                     QSMGIPNRIPYPAGARLLTRGLKRVNARLLPRNGLSQSRYGLFELFSAGDVSVLCSSG
                     VGGGSHVYTALNDRPRVERYWDGRHPEVSDAAMAPHYERVMAEMGGRPPRLEDAIPNL
                     TAQRYRDSSLFRAEDTLQRPAHAVQFPETPGTSREIDWGEGIRRREADWKDGGVLGSR
                     FGTKTTLDFAFLAPAMRRGLRVLDLHEAVSVHLCRQTHAARYCVQTRNLYTGRIEPFY
                     AEHVMLAAGTMNTLRLLLVSRDEARGLDGMPVLGRRFGTNGDCVGWWALDDAEADFSR
                     GLPSHGTIAPRDGARADDPLLIEIGLIGLDRLPMPEALRRKLRRGVMVVSMGADQADG
                     RVSLQRGRLRVRYDRDASPVYGRAEAIFRGLAEASGKKLRVRRRPFTVHPLGGAVLGA
                     SGTEGVTDAGGEVHGHPGLFVLDAAALPAAPGGPPSMTIAAWSSWVAARFLEARS"
     gene            3828..4085
                     /locus_tag="PLS34_09675"
     CDS             3828..4085
                     /locus_tag="PLS34_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87775.1"
                     /translation="MKHAVDLHRRRIPLRLLFIDAVGAVLVAIGILDLLQAGPQLVPA
                     FLQFPGIGIALVVAGSLLMLPVPVWLLRRHQADHRAQGQRQ"
     gene            4082..4987
                     /locus_tag="PLS34_09680"
     CDS             4082..4987
                     /locus_tag="PLS34_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRP87776.1"
                     /translation="MSRHLRRYRILDVFTDTAYGGNPLALLPEAEGLNGRQMQLIARE
                     FNLSETAFVLPPRNKGETHRLRIFTPTRELPFAGHPTVGAALALAEEGWTDTEFVFGE
                     GVGPVRVTLRQQKHLKSAWLWAAKLPEKGPNPPPADALAAVISLRTTDLLAGGWGPAM
                     VSAGVPYLVVPVRDTDALARAELDFNRWREILKDSWADQVYLVAPLDGPHGTRYRARM
                     FAPGIGIPEDSATGSAATAFPGWLVPRLGLKDGIFTIQIEQGIEMGRPSALMIEVELK
                     GGAITVVRVGGTAVSIAEGTIHIPE"
BASE COUNT          754 a         1756 c         1768 g          739 t
ORIGIN      
        1 gatcagccgg tcagtgcgtc gccagccggt gcgtggagtc ggcgcttctc cggctgtgtc
       61 ctcggtgcgc ttcatcccgt ccggggtcag ctcgaaggcc cacgccagga acagcgccag
      121 cggcaggccg agcagcagca gcgcgatcac cagggtgacc gtccactcgg gcaagcccag
      181 ccgcggaaag atggtgtcgc tgacctcgat cagcagccag gcgatgactg cataggcggc
      241 ggcgacacgc accacgttgc ggcgcttcag ctccgcccat agcgaggttg gctcactcat
      301 cgctcgcact ccatccctgg tggcggcccc gcggtgcgcc ggcagaaatc cggctcgccg
      361 aagcggtccc agaaagcggt cagtccgtcg cgcgccgcca actccatgaa gcgcggatgc
      421 tcgcggaatg cccggtccac ggacgaccat atgagcatca gcaggatctg gccggaaacg
      481 ggcttgggcc gcagcgcctc gttgatggcc tcgtccggca gcccggccaa caggtagagc
      541 tgcgcccact cgttctcggg ccaggattgc acggccgcat caatggccgc gcgagccgcc
      601 tcgcgccgcg ccggttcacg gatggcgggc agcaccgctt cgtgcgtggc gcgatgctcg
      661 ggcgcaatgc ggccgtcgtc gtgcagccag tcctcgaagc ggcgcgccat gtcggcccca
      721 ggctccccgg ccaacgccag cttgaggcgc atgaagctgg cgggaccacg ccagccgagc
      781 gcatgggcgc gcttcaggtt cgcctcggcc accgcgtgtt ccccgccgaa cagctgcgcg
      841 gtgcccagcc agccgacgtg gatgccgctc agcggatcga gccgcatggc atgctcgaaa
      901 gcctcccgcg cggcgccgat gtgcccggtc tcgatcagca ccagcccgcg ccacgtccag
      961 gtgttggcat tgttcgggtc cgactcgagg gcgcgttcga tcagctcggc ggccgcgacg
     1021 cgatcgccgc gctccgccgc gatccgcccg ctgacggcca gcgcgtcggg ctggcccggc
     1081 agcaatgcca gcgcgcgctc ggccgcggcg cgggcacgcc ccaccgcctc gtcgccggaa
     1141 agctccgacg agtaggaggg cgtgacatag agcgcgcccg ccagcgtggc ccacgcctcc
     1201 gcaaagccgg gatcgcgcgc caccgcctgc tccagcagct cgcgcgccgc ggccaggttg
     1261 gcgccgcgct gggcgaacag ctgccgcccg cgcaggtaaa gctcgtaggc ttgcagatcg
     1321 gcggtggcct ggcgtacctc caccttgcgc acgccaagcg tgtccgccag cgcatcggtg
     1381 attgcctgcg cgatctcctc ctgcaggctg aaaatgtcgg tcagctgccg gtcgaaagtg
     1441 cccgaccaga gctgctggtc agttgcggcg gccaccagct gggcggtgat gcgcagctgc
     1501 tcgccctgcc tgcgcaccga gccgtcgagc acgtgcgcca cgccgagctg gcgaccgatc
     1561 tcgcgcgagc cgagccgctg gtcgcgcagc gagaacgatg aggtgcgcga ggcgaccttc
     1621 agtccctcga cgcgggccag cacgttgagg atctcctccg ccatgccgtc cgcgagatag
     1681 gcgttctcgg gatccgggct gagattggcg aatggcagca ccgcaatgga aagcgggtcc
     1741 accggacccg gcggagccgg cggccgcggg tcgggcgccg ggaccggggc tggcgcgggc
     1801 gcctgcacct gggtcggcgg cgtgacccgc tgcggcagta cgaactgctt gacggccgaa
     1861 aagcccaccg cgagcagcaa cagcaccagg gtgagctggt cggtgcgccg gccggcgcgt
     1921 gccgtgaccg ggtcgtcccc ctgtgggccg cggcgcaacc tgaggccgtc gggcgtaagc
     1981 cggaacagcc aggcaaagag catcaccggc aacagcgcca gcgccaggcc gatgatcagc
     2041 cagcgcacga ccgcgtcggg aaaaccgaag gccgggaaca gcaggtcggc gatggcgatc
     2101 agcagccaag cgagcgccag ccatgccagc ccggcacgga acacgttgcg gcgcctcaac
     2161 tcggcaaaca gaccggccgt actctcctcc ctgcgcgcaa aacccggagg cgggctcttg
     2221 gcggccctgc cgggtgacgt tcaccccccc tgatcgcgcg actgcctgat tctacgcgca
     2281 ttcgcgtata ttcttcacat ccaaggagga cggatggccc gcgtacacga ggtgctgatc
     2341 atcggcagcg gtttcggggg cagcatcgcg gccgcgcggc tggccgaggc cggcgtggat
     2401 gtcacgctgc tggagcgcgg cccgtggcgc gacaccctgc cggtgcagtc catgggcatc
     2461 cccaaccgga ttccctaccc ggccggcgcc cgcctgctga ccaggggatt gaagcgggtc
     2521 aacgcgcggc tcctgccgcg caacggcctg tcgcagtctc gctacgggct gttcgagctg
     2581 ttctcggccg gcgacgtctc ggtgctgtgt tcctcaggag tcggcggcgg cagccacgtc
     2641 tacacggcgc tcaacgaccg gccccgtgtg gaacgctact gggacggccg tcatcccgag
     2701 gtgtccgacg ccgccatggc gccccactac gagcgcgtca tggccgagat gggcggccgc
     2761 ccaccccggc tggaggacgc catacccaac ctgaccgccc agcggtaccg cgactcgagc
     2821 ctgttccgtg ccgaagacac cctgcagcgc ccggcgcatg cggtgcagtt ccccgagacg
     2881 ccgggcacca gccgcgagat tgactggggc gagggcatcc gccgccgcga ggccgactgg
     2941 aaggacggcg gggtgctggg ctcgcgcttc ggcaccaaga ccacgctgga cttcgcgttc
     3001 ctggcgccgg cgatgcgccg cggactgcgg gtgctcgacc tgcacgaggc ggtctcggtg
     3061 cacctgtgcc ggcagaccca tgcggcgcgc tactgcgtcc agacccgcaa cctgtacacg
     3121 ggccggatcg agcccttcta cgccgagcac gtgatgctgg ccgccggcac catgaacacc
     3181 ctgcggctgc tgctggtgag ccgcgatgag gcgcgcggtc tcgacgggat gcccgtcctc
     3241 gggcgacgct tcggcaccaa cggcgattgc gtcggctggt gggcgctcga cgacgcggaa
     3301 gcggatttca gccgcgggct gcccagtcat ggaacgatcg cgccgcgcga cggcgccaga
     3361 gccgacgatc ccctgctcat tgaaatcggg ctgatcggcc tggatcgact gccgatgccc
     3421 gaggcgctgc ggcgcaagct ccgtcgcggc gtgatggtgg tcagcatggg tgccgaccag
     3481 gccgacgggc gggtgagcct gcagcgtgga cggttgcggg tgcgttacga ccgcgacgcc
     3541 agccccgtat acggacgagc cgaggccatt tttcgaggcc tggcggaggc ctcgggcaag
     3601 aaattgcgcg tgcggcgacg gccgttcacc gtccatccgc tcggcggcgc ggtgctgggg
     3661 gcgagcggca cggagggcgt tactgatgca ggtggtgagg ttcacggcca tcccggcctg
     3721 ttcgtgctgg atgccgcggc cttgccggcg gcgccgggcg gcccgccgtc gatgaccatc
     3781 gccgcctggt cgagttgggt tgcagccagg tttctcgagg cgcgttcgtg aagcacgccg
     3841 tggacctgca ccggcgacgc atcccgctgc gcctgctgtt catcgacgcc gtcggcgccg
     3901 tgctggtcgc gatcggcatc cttgacctgt tgcaggccgg gccacagctg gtgccggcgt
     3961 tcctgcagtt cccgggcatc ggcatcgcgc tggtggtggc cggcagcctg ctgatgctgc
     4021 cggtgccggt ctggctgctg cggcgacacc aggccgacca ccgcgcacag ggacaacgcc
     4081 agtgagccgg cacctcaggc gctatcgcat cctcgacgtc ttcaccgata cggcttacgg
     4141 cggcaatccg ctggcgctgc tgccggaggc ggaaggcctc aacgggcggc agatgcaatt
     4201 gattgcgcgc gagttcaact tgtccgagac ggccttcgtg ctgccgcccc gcaacaaggg
     4261 ggagacgcac cgcctcagga ttttcacccc gacacgcgag ctgccgtttg ccgggcaccc
     4321 gaccgtggga gcggcgctgg cgttggccga agagggctgg accgacaccg aattcgtgtt
     4381 cggcgagggc gtgggacccg tgcgcgtcac gctgcgccag cagaagcacc tgaagagcgc
     4441 ctggctgtgg gccgccaagc tgcccgagaa gggaccgaat ccgccgccgg ccgacgcgtt
     4501 ggctgcagtg atctcgctgc gcaccaccga cttgctggcg ggcggctggg gtcccgccat
     4561 ggtgtcggca ggcgtgccgt acctggtagt tccggtgcgc gatacggatg cactggcgcg
     4621 cgccgagctg gatttcaatc gctggcgcga aattctcaag gactcctggg ccgaccaggt
     4681 ctatctggtg gcgccgctgg acggcccgca cgggacgcgc taccgggcgc gcatgtttgc
     4741 gccgggcatc gggattcccg aagactcggc caccggatcc gcggccactg cctttcctgg
     4801 ctggctggtt ccccggctcg ggctcaagga tggcatcttc accatccaga tcgaacaggg
     4861 catcgagatg gggcgcccca gcgctctgat gatcgaggtt gagctcaagg gcggcgccat
     4921 tacggtcgtg cgcgtcggcg ggacagccgt ttccatcgcc gaaggtacca tccacattcc
     4981 tgagtgaggt ctccaggcca tgcccaggaa gttttca
//
LOCUS       DAOMPG010000181         4940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7167_length_4940_cov_4.451382, whole genome shotgun sequence.
ACCESSION   DAOMPG010000181 DAOMPG010000000
VERSION     DAOMPG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_4940_cov_4.451382"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLS34_09685"
     CDS             complement(<1..227)
                     /locus_tag="PLS34_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRP87777.1"
                     /translation="MSQKFELIAEDRPDQGKGASRRLRRTGKVPAILYGGHRDPRAIA
                     LDHNAMLHNLENESIFSSILTLTVGDKSQPC"
     gene            complement(333..1289)
                     /locus_tag="PLS34_09690"
     CDS             complement(333..1289)
                     /locus_tag="PLS34_09690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRP87778.1"
                     /translation="MQETGHMAVFSGNANRELAESIARHLGFPLGKLQVGRFSDGEVS
                     AEVLENVRGRDVFIVQSTCPPVNDHLLELLIMVDALRRASAARITAVIPYYGYARQDR
                     RPRAQRVAISAKLIANMITHAGVDRVLTVDLHADQIQGFFDIPVDNVYASPVLLHDAW
                     KQRYQNMIVVSPDVGGVVRARALAKRLDDADLAIIDKRRPRANEAQVMHIIGDVRGKT
                     CLLVDDMIDTAGTLVQGVAALKKHGAEKVYAYITHPVLSGPAVQRINESLLDELVVTD
                     TIPLSDAARASPKIRQLSVAEMLAETMRRISDEESVSSLYVD"
     gene            complement(1354..1428)
                     /locus_tag="PLS34_09695"
     tRNA            complement(1354..1428)
                     /locus_tag="PLS34_09695"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1394..1396),aa:Gln,seq:ttg)
     gene            complement(1456..2304)
                     /gene="ispE"
                     /locus_tag="PLS34_09700"
     CDS             complement(1456..2304)
                     /gene="ispE"
                     /locus_tag="PLS34_09700"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRP87779.1"
                     /translation="MSPAWPAPAKLNLFLHVTGRRPDGYHTLQTVFQLLDYCDELFIE
                     VTRDGAIRRPEGASGVAPEDDLVVRAARRLQRASGCRLGAEIRVHKRIPAGGGLGGGS
                     SDAATVLAALNRLWGLDWSPVQLAELGLQLGADVPVFVHGRSAWADGVGERLVPIELP
                     PAWYAVVCPGCAVSTAAVFSDPELTRDTPETTISGFLLAGGRNDCEPVVRRRYPGVAA
                     ALDWVAARSGDGAARLTGTGACVFTRCGTAAEAQRMLAELPRGWTGFAARGLAVSPLL
                     KRIGAA"
     gene            complement(2301..2918)
                     /gene="lolB"
                     /locus_tag="PLS34_09705"
     CDS             complement(2301..2918)
                     /gene="lolB"
                     /locus_tag="PLS34_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HRP87780.1"
                     /translation="MRRRFSIIVLIAVLAAGCTGPPERPGAPRTDWLERRDMLREIEE
                     FRMEGRLALNTGRRGYSGTVSWEQNDDIVDFRFRGPFGFGGFRIHGDPEQLRVKTTAG
                     EEFLLRDPELEMTERFGWSLPVHSMRYWILGVSDPALPAAEVPGDDGLLMSIEQGGWF
                     VQYDGYTESEGLLLPRRLDLEHGEVRIRVMTDRWDIPGARTLARR"
     gene            complement(2961..4637)
                     /locus_tag="PLS34_09710"
     CDS             complement(2961..4637)
                     /locus_tag="PLS34_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP87781.1"
                     /translation="MKPLLPLVLVMLAACASPGPATPDPDELAPDFGSPGEAGYHLLM
                     AEIALQRDRPALAATEYLRAARASDDAEIAARATQVISAYGTRDEGLEAARRWAELAL
                     DDMQPRRYLVRLHLQGGDAERAAAELAALRAAVDPAAVEHPFLALLPLVLETDDRNAA
                     LGAMAAVVADYPADASGAYAHAYLALRSGDPALAQAEATRALGLAPAWTDAALLYARA
                     LAADGRTDEALDWLAARPEAQERGVRLERALLLIAAERSDEARRLLQELLAEVPDDAE
                     ALRALGYLEYFDQQPAAAREAFMALLASGQYTDDALYYLGGIAEQQGDIEEAAHLYSR
                     IEGGEHLVTAQVRLALLMFRMGRPELAINHLELFARRNPAAELELGGARAELLVRLGL
                     QDEALVAYDELLEHHADDFNVRYGRGLLLVQLGRIDDALDDFGHLVALYPDDPTALNA
                     LGYTLTDLTERHDEAYELISRALGQAPDSPAILDSMGWVLYRLGRPGEGLAYIERAWE
                     IQPDEEIAAHLGELLWSLGRVDEAREIWLQAIVEWPGSEILLDTMGRLDP"
     gene            4777..>4940
                     /gene="hemA"
                     /locus_tag="PLS34_09715"
     CDS             4777..>4940
                     /gene="hemA"
                     /locus_tag="PLS34_09715"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="catalyzes the formation of glutamate-1-semialdehyde
                     from glutamyl-tRNA(Glu) and NADPH; the second step of the
                     pathway is catalyzed by glutamate-1-semialdehyde
                     aminomutase which results in the formation of
                     5-aminolevulinic acid; functions in porphyrin
                     (tetrapyrroles) biosynthesis; the crystal structure showed
                     a C-terminal dimerization domain that appears to be absent
                     in Chlamydial proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRP87782.1"
                     /translation="MSLKVLGVNHRSAPVEIREKLAFNPERLPAALRELVQLPQVDEM
                     LIVSTCNRTE"
BASE COUNT          768 a         1764 c         1624 g          784 t
ORIGIN      
        1 atgcacggct gggacttgtc gcccaccgtg agcgtcagga tgctggagaa gatcgactcg
       61 ttctccaggt tgtgcagcat ggcgttgtga tccagcgcaa tggcgcgcgg gtcacggtga
      121 ccaccgtaca ggatggcggg caccttgccg gtccgacgca ggcggcggct cgcacccttg
      181 ccctggtcag gccggtcttc ggcaataagc tcgaatttct ggctcatctg ggttctccgt
      241 tgatgtgatg ttccggcagc gcgggcccgc gaccaggctt gcgcgcatgc cgtcccgacg
      301 acccgactcc ggggcgccgt taccctgtca gctcagtcca cgtagagcga gctgacggat
      361 tcctcgtcgc tgatcctgcg catggtctcg gccagcatct ccgccacgct gagctggcgg
      421 atcttggggc tggcgcgcgc agcgtcggac agcgggatgg tgtcggtgac caccagctcg
      481 tccagcagcg attcgttgat gcgttgcacg gccggccccg acaggaccgg gtgagtgatg
      541 taggcataga ctttctcggc gccgtgcttt ttcagcgccg cgacaccctg gaccagggtg
      601 ccggcggtgt cgatcatgtc gtccaccagc aggcaggtct tgccgcgtac atcgccgatg
      661 atgtgcatca cctgggcctc gttggcccgc gggcgacgct tgtcgatgat agccaggtcg
      721 gcgtcgtcca gccgcttggc cagcgcccgg gcgcgcacta cgccgccgac gtcgggcgag
      781 accacgatca tgttctggta gcgctgcttc caggcatcgt gcagcagcac cggcgaggcg
      841 taaacgttgt ccaccgggat gtcgaaaaag ccctggatct ggtcggcatg caggtcgacg
      901 gtcagcaccc ggtcgacacc ggcgtgggtg atcatgttcg cgatgagctt cgccgagatc
      961 gccacgcgct gggcgcgcgg acgacgatcc tggcgcgcat atccgtagta agggatgacg
     1021 gcggtgatgc gtgccgccga cgcccgccgc aaggcatcca ccatgatgag cagttcgagc
     1081 aggtggtcat tcaccggcgg gcaggtcgac tggacgatga acacgtcgcg gccgcgcacg
     1141 ttctccagca cctcggcgct gacttcgccg tcgctgaaac gccctacctg cagcttgccg
     1201 aggggaaacc ccaggtgccg cgcgatgctt tcggccagtt cccggttggc gttccccgag
     1261 aacaccgcca tgtgccctgt ttcctgaatc acgctcgctc ccctggccgc ctcgtccgcc
     1321 gccgctccgt cgtcaccccg gcgtcacctg aaatggctgg ggtgggagga ttcgaacctc
     1381 cgcatgacgg gatcaaaacc cgttgcctta ccacttggct acaccccagc ggtcaatcgt
     1441 ctgccggcgc cgcggttacg ccgccccgat gcgcttcagc agcgggctta cggccagccc
     1501 gcgcgccgcg aatcccgtcc aaccgcgcgg cagctccgcc agcatccgtt gcgcctctgc
     1561 agccgtcccg caccgcgtga acacgcaggc gccggtgccg gtcagccttg cggcgccgtc
     1621 gccggagcgc gcggcaaccc agtcgagcgc cgccgcgacg cctggatatc gccgccgcac
     1681 caccggctcg cagtcgttgc gccccccggc caaaagaaag ccgctaattg tcgtttccgg
     1741 cgtatcgcgt gtcaattcag gatccgagaa caccgcggcc gtgctgaccg cacaaccggg
     1801 gcagacgacg gcgtaccagg ccggcggcag ctcgatcggc accaggcgct cgccgacgcc
     1861 gtcggcccac gcgctccgcc catgcacgaa taccggcacg tcggcgccca gctgcagccc
     1921 gagttcggcc agttgcacgg gcgaccagtc cagcccccac agccggttga gcgccgccag
     1981 caccgtggcc gcatccgagc tgccgccgcc gaggccgccg ccggcgggaa tccgcttgtg
     2041 cacccgaatc tcggcgccca ggcggcaacc cgaagcccgt tgcagccggc gggcggcacg
     2101 gaccacgaga tcgtcctccg gcgccacgcc cgacgccccc tccggccgcc gtatggcgcc
     2161 atcccgggtg acctcgatga acagctcgtc gcagtaatcg agcagctgaa agaccgtctg
     2221 caaggtgtgg tagccgtcgg gccggcggcc ggtgacgtgc aggaagaggt tgagcttggc
     2281 cggcgcaggc caggccgggc tcatcggcgc gcgagcgtcc gcgcgccggg gatgtcccag
     2341 cggtcggtca tcacccggat gcggacttcg ccgtgttcca ggtccagccg ccggggcagg
     2401 agcaggcctt ccgactcggt atagccgtcg tactgcacga accagccccc ttgttcgatg
     2461 ctcatcagca ggccgtcatc gcctggcacc tcggcggccg gcaacgccgg atcggagacg
     2521 cccaggatcc agtagcgcat gctgtgaacc ggcaggctcc agccgaagcg ctcggtcatc
     2581 tcgagctcgg gatcgcgcag caggaactcc tcgccggccg tggtcttgac ccgcaactgc
     2641 tccgggtcac cgtggatacg aaagccaccg aagccaaagg ggccgcggaa gcggaaatcc
     2701 acgatgtcgt cgttctgctc ccaggagacc gtgccgctgt aaccgcgccg ccccgtgttg
     2761 agtgcgagcc gaccctccat ccggaactct tcgatttcgc gcagcatgtc ccgcctttcc
     2821 aaccagtcag tcctcggcgc cccggggcgt tcgggcggcc cggtgcagcc cgccgccagg
     2881 acggcaatga gaacgattat agagaacctt ctccgcatgt cggcagtggc ccgcgccgtg
     2941 cgatcccgtc gcagcatttc tcaggggtcc agccgcccca tggtgtccag cagaatctcg
     3001 ctgccgggcc attccacgat cgcctgcagc cagatctcgc gtgcttcgtc gacacggccg
     3061 aggctccaca gcagctcgcc caggtgcgct gcaatctctt cgtccggctg gatctcccag
     3121 gcacgctcga tgtaggccag gccttccccg ggtcgtccca gccggtacag cacccagccc
     3181 atgctgtcca ggatcgccgg gctgtccggc gcctggccga gcgcccggga aatcaactcg
     3241 taggcctcgt cgtggcgctc ggtcaggtcg gtcagcgtgt agccaagcgc gttcagggcg
     3301 gtcgggtcgt ccggataaag cgccaccagg tggccgaaat cgtccagcgc gtcatcgatc
     3361 cgccccagct ggaccagcag caggccccgc ccgtaacgaa cgttgaaatc gtccgcatga
     3421 tgctcgagca gctcgtcata ggccaccagc gcctcgtcct gcaggcccag ccgcaccagc
     3481 agttcggcgc gcgcgccgcc cagttccagc tcggccgccg ggttgcggcg cgcgaacagc
     3541 tccaggtgat tgatggccag ctcaggccgg cccatgcgga acatcagcag tgcgagccgc
     3601 acctgtgcgg tgaccaggtg ctcgccaccc tcgatgcgag agtacaggtg ggccgcttcc
     3661 tcgatatcgc cctgctgctc ggcgatcccg cccaggtagt acagggcgtc gtcggtgtac
     3721 tgcccgctgg cgagcagggc catgaaagcc tcgcgcgcgg ccgccggctg ctggtcgaag
     3781 tattcgaggt atcccagcgc gcgcagcgcc tcagcgtcgt ccggaacctc ggcgagcagt
     3841 tcctgcagta gtcgccgtgc ctcgtcgctg cgttccgccg cgatgagcag cagggcacgc
     3901 tccagccgta cgccgcgctc ctgcgcctcg ggccgcgccg ccagccagtc cagcgcctcg
     3961 tccgtgcgtc cgtcagcggc cagcgcccgg gcatacagca gcgctgcatc cgtccacgcg
     4021 ggggcaaggc cgagcgcgcg cgtcgcttcc gcctgggcga gcgcagggtc cccgctacgc
     4081 agcgcgagat aggcatgcgc ataggcgccg ctggcgtccg ccgggtagtc ggcgaccacc
     4141 gcggccatgg ccccgagcgc agcgttgcga tcgtcggttt ccaataccag cggcagcagc
     4201 gcgagaaagg ggtgttcgac cgccgccggg tcgaccgcag cccgcagcgc tgcgagctcg
     4261 gccgcggccc gctccgcgtc gccgccctgc aggtgcaagc gcaccaggta gcgtcgtggc
     4321 tgcatgtcgt ccagcgccag ctcggcccag cgccgggcag cctccaggcc ctcgtcgcgc
     4381 gtgccgtagg cactgatgac ctgggtcgcg cgcgcggcga tctcggcatc gtcgctggcc
     4441 ctggcggccc gcaggtactc cgtcgcggcc agcgccgggc ggtcgcgctg caaggcgatc
     4501 tcggccatca acaggtgata gccggcctcg ccggggctgc cgaagtccgg tgccagctcg
     4561 tcggggtccg gggtagccgg tccgggcgaa gcgcaggcgg ccagcatcac caggacaagt
     4621 gggagaaggg gtttcatgtt cattttcggc gagtatcgga aagccactat accccatgaa
     4681 aacaaggccc cgtgagttgc cacaacttgt aaccgcgggg ccgcttgccg gacaattgtc
     4741 caatcatcca accgggcggc tgcgccacca gaccagatgt ccctgaaagt cctcggcgtc
     4801 aatcatcgct cggctccggt cgagatccgg gagaagcttg ccttcaaccc ggaacggttg
     4861 cctgcggcct tgcgggagct cgtgcagctg ccgcaggtgg acgagatgct gatcgtctcg
     4921 acctgcaacc ggaccgagtt
//
LOCUS       DAOMPG010000182         4902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7232_length_4902_cov_4.048484, whole genome shotgun sequence.
ACCESSION   DAOMPG010000182 DAOMPG010000000
VERSION     DAOMPG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7232_length_4902_cov_4.048484"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..481
                     /gene="erpA"
                     /locus_tag="PLS34_09720"
     CDS             119..481
                     /gene="erpA"
                     /locus_tag="PLS34_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRP87783.1"
                     /translation="MATHMTETNAPGHPLVFTDAAATKVGQLIKEESNPNLKLRVFIQ
                     GGGCSGFQYGFTFDENEDEGDTRIENCGVTLLVDPMSVQYLQGAEIDYREDLNGAQFV
                     IRNPNASTTCGCGSSFGV"
     gene            517..2016
                     /gene="ppx"
                     /locus_tag="PLS34_09725"
     CDS             517..2016
                     /gene="ppx"
                     /locus_tag="PLS34_09725"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRP87784.1"
                     /translation="MTADSQPDVIAAVDLGSNSFHMVVARRSNGELTILDRIREMVRL
                     GAGLDENGRITAEAAEAALACLGRFRERLAALEAAQVRAVGTNTLRRARRRNAFLSRA
                     RGALGHPIEVISGVEEARLVYLGAAHSLPSQVGRRLVVDIGGGSTELIIGEGYEGLRL
                     ESLYMGCVSSSETFFPGGVVTERGFKEARLFARRELEPVRAPFRQVDWQQAVGTSGTI
                     RATGRLLRLLGVTGGEITRPGLELLRERLLAAGSMAHHDLPGLSAQRAPVYPGGLAVL
                     TEVFDALEIETMLPAEGALREGLLWDLVGRLTDEDARDRSVRSFQQRFHVEIAHAERV
                     EATALALHGQLAGTWSLDGPECTELLGWAARLHEVGLDIAHAQHQLHAAYLLRNADLA
                     GFSFDQQRRLAVLVGGHRRKIREELLDGLQEPWSKRIPYLLAILRLAVLLHRSRGPAP
                     PPPIEIEAGKRKLRLRFPDGWLDSHPLTRADLEQESRYLGALDLKLSFS"
     gene            complement(2009..4102)
                     /gene="ppk1"
                     /locus_tag="PLS34_09730"
     CDS             complement(2009..4102)
                     /gene="ppk1"
                     /locus_tag="PLS34_09730"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRP87785.1"
                     /translation="MSAATADTSPLQRPELYLNRELAALEFNRRILELASDPQVPPLE
                     RLRFLCLSCTSLDKFFEIRVGGLKERIERGASYLRRDGLSPADTLAGIAQVARGIVAE
                     QYRVFNESLGPELAARGVRFRPRGDWNKAQTRWARRLFRDEVLPVLTPIGLDPAHPFP
                     RILNKSLNFIVELQGLDSFGRDAPLAVVQAPRSLARIIRVPATVAGTGSDDFVFLSSV
                     IHAFVGELFPGLEVLGCHQFRVTRNSDLLVDDEEAEDLLRAVEGELASRPYGDEVRLE
                     VAQGCPSHLLRFLLNRFGLGDHDLYEVNGPVNLNRLSQVIALVDRAELKYPAFVPGRP
                     RELARAGSVSAAMRRGDILLHHPYQSFAPVIELLREAAVDPAVLAVKQTLYRTGPDSP
                     VVDALVEAARAGKEVTVVVELMARFDEAENIALAARLQAAGAHVVYGVVGYKTHAKML
                     LIVRRENGRLKRYVHLGTGNYHPETARSFADYGLLTCNSRIGRDVHQALMLLTSPGVR
                     PTTRALTLAPLGLHEHIIGLVRREAEHAAAGRPARIVAKLNAVVEPEVIEALYAASCA
                     GVEIDLIVRGTCCLRPGVPGVSENIRVRSLVGRFLEHARVLMFGNGGATEILLSSADW
                     MERNFFRRVEVCFPVTRQALKRRLAADLEAELRDNRCAWEMQPDGRWERIATPLADAV
                     SVQELRLAALAQRLS"
     gene            complement(4099..>4902)
                     /locus_tag="PLS34_09735"
     CDS             complement(4099..>4902)
                     /locus_tag="PLS34_09735"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004862682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRP87786.1"
                     /translation="RPFTLQVGDPNIIAARTGTVVVADFRRRDMALGGQGAPLVPAFH
                     QAVFGHPAERRAVLNLGGVANLTLLAPGEAVAGFDTGPANGLMDAWARRHLGRPFDAN
                     GAWAATGTVSAPLLAALLAHPFLALPAPKSTGPEEFTPGWLDSAIGQAGHPAPPDVQA
                     TLCEFTAVAIAAALRDAPPQRLIVCGGGVHNGHLLARLAARLPGTTVTASSAHGIDPD
                     HVEAAAFAWLAARTLAGLPGNLPTVTGAARPAVLGAVWPGEAGAPARQS"
BASE COUNT          732 a         1703 c         1785 g          682 t
ORIGIN      
        1 cggccagcag gcgctgccgg cgccggacga ggccaaagct gcgccggccg ccgatgagga
       61 cgagtccgac cccgtgcggt agaatggcgc ctaatgagtg gcttttgcga gcgaatccat
      121 ggcgacccac atgactgaaa cgaacgcccc cggccacccg ctggtgttca ccgatgccgc
      181 ggcgaccaag gtcgggcagc tgatcaagga agagagcaac cccaatctca agctgcgcgt
      241 cttcatccag ggcggcggct gctcgggctt ccagtacggc ttcacctttg acgagaacga
      301 ggacgagggc gacacgcgca tcgagaactg cggcgtgacg ctcttggtcg acccgatgag
      361 cgtccagtac ctgcagggtg cggagatcga ctaccgcgag gacttgaacg gcgcgcagtt
      421 cgtgatccgc aacccgaacg catccaccac ctgcgggtgc ggatcgtcgt tcggagtctg
      481 aacggcgcga cgcggatcct ctgaggtgcg gcccaggtga cggcggacag ccagcccgac
      541 gtcatcgccg ccgtcgatct cggttccaac agctttcaca tggtggtggc tcgccgcagc
      601 aacggcgagc tgaccatcct cgaccgcatc cgtgaaatgg tccggctcgg cgccgggctg
      661 gacgagaacg ggcggatcac ggccgaggcg gcggaggccg cgctcgcctg cctggggcgc
      721 ttccgcgaac gcctcgccgc gctcgaggcg gcccaggtca gggcggtcgg caccaacacg
      781 ctgcgccggg cgcgccgccg caacgctttc ctgtcacgcg cccgcggcgc gctcggccac
      841 cccatcgagg tgatctccgg cgtcgaggag gcacggctgg tctatctcgg tgccgcccac
      901 agcctgccct cgcaggtcgg ccgccggctg gtggtggaca tcggcggcgg cagcaccgag
      961 ctgatcatcg gcgaaggcta cgagggactg cgtctcgaaa gcctctacat gggctgcgtc
     1021 agcagcagcg agacattttt tcccggcggc gtcgtcaccg agcggggctt caaggaggcg
     1081 cggttgttcg cccgccgcga gctggagccg gtgcgcgcgc ccttccggca ggtcgactgg
     1141 cagcaggcgg tgggtacttc cggcaccatt cgcgccaccg gccgcctgtt gcgcctgctc
     1201 ggcgtgaccg gtggcgagat cacccggccg gggctcgagc tgctgcgcga acggctgctg
     1261 gccgccggca gcatggcgca ccatgatctg ccgggcctga gcgcccagcg ggcgcccgtc
     1321 tatccgggcg gcctggccgt gctgaccgag gtgttcgacg cgctggagat cgagaccatg
     1381 ctgccggccg agggcgcgct gcgcgaaggc ctgctgtggg acctggtcgg ccggctgacg
     1441 gacgaggacg cccgcgatcg cagcgtgcgc agcttccagc agcgcttcca tgtcgagatc
     1501 gcccacgccg agcgcgtcga ggccacggcg ctggcactgc acgggcagct ggcgggcacc
     1561 tggtcgctgg acgggccgga gtgcaccgag ctgctcggct gggcggcccg cctgcacgag
     1621 gtcgggctgg acatcgcgca cgcccagcat cagctgcatg cggcctacct gttgcgcaat
     1681 gccgacctgg cgggattttc tttcgaccag cagcgccgcc tcgccgtgct ggtgggcgga
     1741 caccgccgca agatccgcga ggaattgctc gacggcctgc aggagccgtg gtcgaagcgc
     1801 attccgtact tgctggcaat cctgcgcctg gccgtgctgc tgcaccgcag ccgggggccg
     1861 gcgccaccac cgccgatcga gatcgaggcc ggcaagcgca agctgcggct caggtttccc
     1921 gacggttggc tggactccca cccgctgacg cgggccgacc tcgagcagga aagccgctac
     1981 ctcggtgcgc tcgatctgaa gctcagcttc agctgagccg ctgagcgagc gcggccagcc
     2041 gcagttcctg gaccgacacc gcgtccgcga ggggcgttgc gatgcgttcc cagcgcccgt
     2101 ccggctgcat ttcccaggcg cagcggttgt cgcgcagttc ggcctccagg tcggctgcca
     2161 ggcgccgctt gagggcctgg cgggtgaccg ggaaacacac ctcgacgcgc cggaaaaagt
     2221 tgcgctccat ccagtcggca ctcgacagca agatttcggt ggcgccgccg ttgccgaaca
     2281 tgagcacgcg ggcgtgctcc aggaagcggc ccaccaggga acgcacgcgg atgttctccg
     2341 agacccccgg cacgccgggc cggaggcagc aggtgccgcg cacgatcagg tcgatctcga
     2401 ccccggcgca ggaagcggcg tacagcgcct cgatcacctc cggctccacc accgcgttga
     2461 gcttggcgac gatgcgcgcg ggccggcccg ccgcggcatg ctccgcttcg cgccggacga
     2521 ggccgatgat gtgctcgtgc aggccgagcg gtgcgagggt gagtgcccgc gtggtcggcc
     2581 gcacgccggg gctggtcagc agcatcaagg cctggtgcac gtcgcgcccg atgcggctgt
     2641 tgcaggtcaa caggccgtag tcggcgaagc tgcgcgcggt ctcgggatga tagttgccag
     2701 tgccgaggtg cacgtagcgc ttcagccgcc cgttctcgcg ccgcacgatc agcagcatct
     2761 tggcgtgggt cttgtagccg accacgccgt agaccacgtg ggcgccggcg gcctgcagcc
     2821 gcgccgcgag ggcgatgttc tcggcctcgt cgaaacgcgc catcagctcg accaccacgg
     2881 tcacttcctt gccggcgcgc gccgcttcga cgagggcgtc gaccaccggc gagtcaggcc
     2941 cggtgcggta cagggtctgc ttgacggcca gcaccgcggg gtcgaccgcc gcttcgcgca
     3001 gcagttcgat caccggtgcg aaggactggt agggatggtg caggaggatg tcgccgcggc
     3061 gcatcgccgc gctgacgctg ccggcgcgcg ccagttccct gggtcgcccc ggcacgaacg
     3121 ccggatattt cagctcggcg cggtccacca gggcgatgac ctgcgacagc cggttcaggt
     3181 tgaccggccc gttcacctcg tagaggtcgt ggtcgccgag accgaagcga ttcaggagaa
     3241 acctgagcag gtgcgagggg catccctggg cgacctccag gcggacctcg tcgccgtacg
     3301 gacgcgatgc gagctcgccc tcgaccgcgc gcagcaggtc ttcggcctcc tcgtcatcga
     3361 ccagcaggtc gctgttgcgc gtgacccgga actggtggca gccgaggacc tcgaggccgg
     3421 gaaacagttc acccacgaag gcatggatca ccgaggacag gaacacgaaa tcgtcgctgc
     3481 cggtgcccgc caccgtcgcg gggacgcgga tgatgcgcgc cagcgagcgc ggcgcctgca
     3541 cgacggcgag cggcgcgtcg cgaccgaagg agtccaggcc ctgcagctcg acgatgaagt
     3601 tgagcgactt gttgaggatc cgcgggaagg gatgtgccgg gtcgaggccg atcggcgtca
     3661 gcaccggcag cacctcgtcg cgaaacaggc ggcgcgccca gcgtgtctgc gccttgttcc
     3721 agtcgccgcg cggccggaag cggacgccgc gggcggcgag ctccggcccg agcgattcgt
     3781 tgaacacccg gtactgctcc gcgacgatgc cgcgcgccac ctgggcgatg ccggccagcg
     3841 tgtcggccgg gctgagaccg tcgcgccgca ggtaggaggc gccgcgctcg atgcgttcct
     3901 tgagcccgcc gacgcggatc tcgaagaact tgtccaggct ggtgcaggac aggcacaaga
     3961 aacgcagccg ctccagcggc ggcacctgtg gatcgctcgc cagctcgagg atgcggcggt
     4021 tgaactcgag cgccgcgagc tcgcgattca ggtacagctc gggccgctgc agcggcgacg
     4081 tgtcggcagt ggcggcgctc acgattgcct tgccggcgcg ccggcctcgc cgggccacac
     4141 ggcgccgagc acggccggac gggcggcgcc ggtcacggtg ggcaggttgc cgggcaggcc
     4201 ggccagggtg cgtgccgcca gccaggcgaa ggcggcggcc tcgacatggt ccgggtcgat
     4261 cccgtgcgcg ctgctggccg tcaccgtggt gcccggcagg cgtgcggcca ggcgcgccag
     4321 caggtggccg ttgtgcacgc cgccgccgca aacgatcagc cgctgcggtg gcgcgtcgcg
     4381 caacgccgcg gcgatggcga cggcggtgaa ctcgcacaag gtggcctgca cgtccggtgg
     4441 cgccgggtgg ccggcttgcc cgatggcgga gtcgagccag ccgggcgtga attcctccgg
     4501 tccggtgctc ttcggcgccg gcagggcaag gaaagggtgt gccagcagcg ccgccagcag
     4561 cggcgccgag accgtgccgg tcgccgccca ggcgccgttc gcgtcgaacg gccggccgag
     4621 gtggcgccgc gcccaggcgt ccatcaggcc gttggccggc ccggtgtcga agccggcgac
     4681 cgcctcaccc ggcgccagca gcgtcaggtt ggcaacgccg ccgagattga gtaccgcgcg
     4741 ccgctcggcc ggatggccga acacggcctg gtgaaaggcc ggcaccagcg gcgcgccctg
     4801 cccgccgagc gccatgtcgc ggcggcgaaa atcagctacc accaccgtgc cggtgcgcgc
     4861 cgcgatgatg ttcgggtcgc ccacctgcag ggtgaacggc cg
//
LOCUS       DAOMPG010000183         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7268_length_4881_cov_4.587650, whole genome shotgun sequence.
ACCESSION   DAOMPG010000183 DAOMPG010000000
VERSION     DAOMPG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7268_length_4881_cov_4.587650"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLS34_09740"
     CDS             complement(<1..241)
                     /locus_tag="PLS34_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRP87787.1"
                     /translation="MSDACRAVRARTVALAAPLSAEDACVQSMPDASPAKWHLAHTTW
                     FFERFVLAVREPGYRVFHERFEYLFNSYYFTVGQMH"
     gene            422..497
                     /locus_tag="PLS34_09745"
     tRNA            422..497
                     /locus_tag="PLS34_09745"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:455..457,aa:Phe,seq:gaa)
     gene            complement(502..2073)
                     /locus_tag="PLS34_09750"
     CDS             complement(502..2073)
                     /locus_tag="PLS34_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRP87788.1"
                     /translation="MSKPALDKDLKRLTRLEHATRGVARGLVPLSLALLFLVVVGIAT
                     ASFVGVSGSLAVIVAASVIGGYMALNIGANDVANNMSPAVGSRALHIGVAVAIAAVFE
                     AAGAILAGGDVVATVSSGIIRPDHMPNTGAFTTAMLAALFAAALWINLATWIGAPVST
                     THSVVGGVLGGGVAMAGLGAADWSVVLRIVASWVVSPVLGGVIAASFLLYIKLTILYV
                     PDQLEQARRRVPLLVAIMAGAFTTYLMLKGFSRVWKAPVWLALSVGGTVLLLTVAWLK
                     PVISRKVKVLPPNKSGVGNLFTIPVICGAALLSFAHGANDVANAIGPLAAIYASVTEG
                     GIVSVAKIPPWILLTGAFGIATGLLLYGPKLIRTVGSKITKLNRVRAFCVTLSAAITV
                     IIASALGLPVSSTHIAIGALFGVGFLREFMANRRARNGLEVMPLDTMPLKADTDFMPD
                     NGDDSDPAEAPEAAASPSQEQGKSPEKLRKRKLVRRSHLLTIIAAWLITVPASAGVAA
                     LIALALARSGLPGLG"
     gene            complement(2274..4100)
                     /gene="recQ"
                     /locus_tag="PLS34_09755"
     CDS             complement(2274..4100)
                     /gene="recQ"
                     /locus_tag="PLS34_09755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRP87789.1"
                     /translation="MPESTLHDDLDRTLASVFGFPSFRPQQREIVAGLVAGRDAFVLM
                     PTGGGKSLCYQVPALHRPGTAVVVSPLISLMKDQVDALLANGVAAAFYNSSLEAAEAR
                     RTLARLHAGELSLLYVAPETLMSDAFLARLDGIEIALFAVDEAHCVSQWGHDFRPEYV
                     QLGGLRARFPGVPMVALTATADPQTREDIVRVLDLGQATRYVASFDRPNIRYTVREKH
                     QPFEQLKRFLATREHQAGIVYALSRKRVDELAAKLEALGLRAGAYHAGLAAAERTRVQ
                     DAFLRDELDIVVATVAFGMGIDKPDVRFVVHHDMPKNIEGYYQETGRAGRDGLPAEAL
                     LLFGAQDVVTARALVERGSSDERRRIELHKLNAMVALAESLTCRRRVLLGYFGERLAQ
                     DCGNCDVCLEPPERFDATDDARKALSCVYRLNQGYGIKYVVDVLRGTDNERIRAAGHD
                     QLSTWGIGAHHGVQEWTSLIRQLIHRGYLEQDIARYSVLRLTPAARPLLRGEETLELA
                     RPRIRTPSRRKAPKAAAGAGRYDETLFEALRVLRKSIADAQGVPPYIIFGDATLIEMA
                     RELPAGPAALLGITGVGQKKLERYGEDFLALIRQHLGTFPNS"
     gene            complement(4150..>4881)
                     /locus_tag="PLS34_09760"
     CDS             complement(4150..>4881)
                     /locus_tag="PLS34_09760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HRP87790.1"
                     /translation="VRQLLDDDDVETAETVLDPARIAGLLSAITFPPLLLNVALPGIR
                     GLFTTALLQVNSAAGRILLDGLSPDEGHALLAPGSVMHVHGKLRGVQLDFATQVIEIQ
                     AGELLPAYLSRMPGSLRYHQRRRHFRLRTGHLHEYSIVNLGPDGGDLRGRLLDLSAGG
                     LKGVFPRNPGLTPGQVVDGCRLSILGEEQLECGIEFIHVQVSPLTGEIEAGARFLGTG
                     SRGTERLQRLLTKLQRQQLRHRIPE"
BASE COUNT          800 a         1635 c         1724 g          722 t
ORIGIN      
        1 agtgcatctg gccgacggtg aaatagtagg aattgaacag gtactcgaag cgctcatgga
       61 atacccggta gccgggctcc cgtacggcga ggacgaagcg ctcgaaaaac caggtcgtat
      121 gggccaggtg ccacttggcc gggctggcgt ccggcatgct ctgcacgcag gcgtcctcgg
      181 ccgagagcgg tgccgcaagg gcgacggtgc gagcccgcac ggcccgacag gcgtcggaaa
      241 tggaggccgc ttgcggcgcg tgctgtgcgg ccgggctgac ggtgctcacg gtcggcgtct
      301 tcaggtctcg gggggaggct cagggtagtc gctggggctc cggcaggtca aaggccggcc
      361 ggcgggcgcc cgccttgacg gccgcgcggc cgcacgggtc taatacgcgc ctcccgccga
      421 cggccaggta gctcagttgg tagagcacgt gactgaaaat cacggtgtcg gcagttcgat
      481 tctgcccctg gccaccactt cctagccgag tccgggcaac cccgagcggg ccagcgccag
      541 tgcgatcaat gcggccacgc ccgcgctcgc aggcacggtg atcagccagg cagcgataat
      601 ggtcagcagg tgcgagcggc gcaccagctt gcgcttgcgc agcttctcag gggacttgcc
      661 ttgctcctgc gaaggcgaag ccgcggcctc tggtgcttcc gcgggatcgg agtcgtcgcc
      721 gttgtccggc atgaagtcgg tgtcggcctt cagcggcatg gtgtcgagcg gcatcacctc
      781 gagcccgttg cgcgccctgc ggttggccat gaattcgcgc aggaagccga cgccgaacaa
      841 ggcgccgatg gcgatgtggg tcgagctcac cggcaagccg agcgccgacg cgatgatcac
      901 cgtgatggcc gccgacagcg tgacgcagaa ggcgcgcacc cggttcagct tggtgatctt
      961 cgagcccacc gtgcggatca gcttgggccc gtagagcagc aggccggtgg cgatgccgaa
     1021 ggcgccggtg agcaggatcc atggcgggat cttggccacg ctgacgatgc cgccttcggt
     1081 gaccgaggcg tagatcgccg cgagcggtcc gatcgcgttg gccacgtcgt tggcgccatg
     1141 ggcgaaggac agcagcgccg cgccgcagat caccggaatg gtgaacaggt tgcccacccc
     1201 tgacttgttg ggcggcagca ccttgacctt gcgcgagatg accggcttga gccatgcgac
     1261 ggtcaagagc agcaccgtgc cgccgacgct cagcgccagc cataccggcg ctttccagac
     1321 ccgtgagaac cccttcagca tcaggtaggt ggtgaacgcg ccggccatga tcgccaccag
     1381 cagcggcacg cgccggcgcg cctgctcgag ctggtccggt acgtacagga tggtgagctt
     1441 gatgtagagc aggaaggagg cggcgatgac gccgccgagc accggcgaga ccacccagct
     1501 cgccacgatg cgcaacacga cgctccagtc ggccgcaccg aggccggcca tcgccacgcc
     1561 gccgccgagc accccgccga ccactgagtg ggtggtggac accggcgcgc cgatccaggt
     1621 cgcgaggttg atccacagcg ccgccgcgaa cagtgcggcc agcatcgccg tggtgaaggc
     1681 gccggtgttc ggcatgtggt cggggcgaat gatgcccgag gagacggttg ccaccacgtc
     1741 gccgccggcg aggatggccc cggcggcctc gaacacggcg gcgatcgcga ccgccacgcc
     1801 gatgtgcagg gcccgcgagc cgaccgccgg cgacatgttg ttggccacgt cgttggcgcc
     1861 gatgttcagc gccatgtagc cgccgatcac gctggccgcg acaatcaccg cgaggctgcc
     1921 gctgacgccg acgaaggagg ccgtcgcgat gcccaccacc accaggaaca gcagcgcgag
     1981 gctcaagggg accaggccgc gcgccacgcc gcgggtcgcg tgctccagcc gtgtaagccg
     2041 cttcagatcc ttgtcgagag cgggttttga catgaaatca gctgcttggg cgcccggccg
     2101 cgaatacgag acaggccggt ctgacaccgg cctgtaacaa aactgtcatt ttcgcggcga
     2161 ttctggagaa gcggccggca aagggcaaga aacaacgggc tcagaaactg gggtcagaca
     2221 ccggtttcgc gatggaaacc ggtgtctgac cccagtttct gagcccggtt gtctcaggaa
     2281 ttggggaatg tccccaaatg ctggcggatc agggccagga agtcttcgcc gtagcgctcg
     2341 agtttcttct ggccgacgcc ggtgatcccg agcagcgccg ccgggccggc cggcagctcg
     2401 cgcgccatct cgatcagggt ggcgtcgccg aagatgatgt acggcggcac cccctgcgcg
     2461 tcggcgatcg acttgcgcag cacgcgcagt gcctcgaaca gcgtctcgtc gtatcttccg
     2521 gcgccggccg cggccttcgg cgccttgcgc cgcgacggcg tgcgaatgcg cggcctggcg
     2581 agctcgagcg tttcctcccc gcgcagcaag ggccgcgcgg ccggcgtcag ccgcagcacc
     2641 gaataacggg cgatgtcctg ctcgaggtag ccgcgatgaa tcagctggcg gatcagcgag
     2701 gtccactcct gcacgccgtg gtgagccccg atgccccagg tggacagctg gtcatggccc
     2761 gccgcgcgga tgcgctcgtt gtccgtgccc cgcaacacgt cgacgacgta cttgatgccg
     2821 tagccctggt tcagccgata cacgcaggac agcgccttgc gcgcgtcatc ggtcgcatcg
     2881 aagcgctcgg gcggctcgag gcacacgtcg cagttgccgc agtcctgtgc cagccgctcg
     2941 ccgaagtagc cgagcagcac gcgccgccgg caggtcaggc tttcggccag cgccaccatg
     3001 gcgttgagct tgtgcagctc gatgcgccgc cgctcgtcgc tgctgccgcg ctccaccagc
     3061 gcgcgggcgg tcaccacgtc ctgcgcgccg aacagcagca gcgcctcggc cggcagcccg
     3121 tcgcggcccg cacggccggt ttcctggtaa tagccctcga tgttcttcgg catgtcatgg
     3181 tgcacgacga agcgcacgtc cggcttgtcg atgcccatgc cgaacgccac ggttgcgacc
     3241 acgatgtcga gttcgtcgcg caggaacgcg tcctggaccc gcgtgcgctc cgcggcggcc
     3301 aatcccgcat ggtaggcgcc ggcgcgcagg ccgagtgcct cgagcttggc cgccagctcg
     3361 tcgacccgct tgcgcgacaa ggcgtagacg atgcccgcct ggtgctcgcg ggtggcgagg
     3421 aaacgcttca gctgctcgaa gggctggtgc ttctcgcgta cggtgtagcg gatgttgggc
     3481 cggtcgaagc tggccacgta gcgcgtcgcc tggccgagat cgagcacccg cacgatgtcc
     3541 tcgcgggtct gcggatcggc ggtcgcggtg agcgccacca tcggcacgcc gggaaaacgc
     3601 gcccgcaggc cgccgagctg cacgtactcc ggccggaagt cgtggcccca ctgcgacacg
     3661 cagtgcgcct cgtccacggc gaacaacgcg atctcgatgc cgtccaggcg ggccagaaac
     3721 gcatcgctca tcagggtttc gggcgcgacg tacaacaggg acagctcgcc ggcatgcagc
     3781 cgcgccagcg tgcggcgcgc ctcggccgcc tccagcgagg agttgtagaa cgccgccgcc
     3841 acgccgttgg ccagcagcgc gtccacctgg tccttcatca gcgagatgag tggcgacacc
     3901 accacggccg tgccggggcg atgcagcgcc gggacctggt agcacaggga cttgcccccg
     3961 ccggtgggca tcaggacgaa ggcgtcgcgg cccgcaacca ggcctgcgac gatctcgcgt
     4021 tgctggggac ggaaggaagg gaatccgaac accgacgcaa gcgtccggtc gaggtcgtcg
     4081 tgtagagtcg attcgggcat cgggctcgca gctgggggga gcggacggcc ctacatcata
     4141 acggctcggc tactcgggga tgcggtggcg cagctgctgg cgctgcagct tggtgagcag
     4201 tcgctggagc ctctcggtgc cgcgcgagcc ggtgccgagg aagcgggcgc cggcttcgat
     4261 ctcgccggtc agcggcgaga cctgcacgtg gatgaactcg atgccgcact ccagctgttc
     4321 ctcgccgaga atgctgaggc gacacccgtc gaccacctgg cccggcgtca gtcctggatt
     4381 gcgcggaaac acgcctttca ggccgccggc ggagaggtcc agcagccggc cgcgcaggtc
     4441 gccgccgtcg ggcccgaggt tgacgatcga gtattcgtgc aggtgcccgg tgcgcaggcg
     4501 gaagtgccgg cgacgctggt ggtagcggag cgagccgggc atgcgcgaca ggtaggccgg
     4561 caacagctcg cccgcctgga tctcgatgac ctgcgtagcg aagtccagct gcacgcccct
     4621 gagcttgccg tgcacgtgca tcacgctgcc cggtgccagc agggcgtgac cttcgtccgg
     4681 gctgaggcca tcgagcagga tgcggccggc ggccgagttc acctgcaaca gcgcggtggt
     4741 gaacaggccg cgtataccgg gcagcgccac gttgagcagc agcgggggaa aagtgatcgc
     4801 actcagcagt cccgcgatgc gcgccgggtc gagcacggtc tccgccgtct ccacatcgtc
     4861 atcgtcgagg agctggcgga c
//
LOCUS       DAOMPG010000184         4839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7336_length_4839_cov_4.482651, whole genome shotgun sequence.
ACCESSION   DAOMPG010000184 DAOMPG010000000
VERSION     DAOMPG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7336_length_4839_cov_4.482651"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /gene="polA"
                     /locus_tag="PLS34_09765"
     CDS             <1..1828
                     /gene="polA"
                     /locus_tag="PLS34_09765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRP87791.1"
                     /translation="ADPSTSETTDYETVLTEPQLDGWLKRLRKAHEFAFDTETDSLDY
                     MRARIVGVSFAVEPGCAAYVPLAHRDPDAPGQLPLEDVLERLRPLLEDADKAKIGHHL
                     KYDRHVLANHGIALEGIRFDTMLESYVLDSTATRHDMDSVALKYLGVKTIHYEDVAGK
                     GAKQISFDQVPVATAAPYAAEDADVTLRLHRELWPRLVATPGLQRIYEELEQPLVPIL
                     GDMERHGVLLDTALLRRQSRELEKGMAAAEAAAFEAAGGKFNIGSAKQLQEILFDRLG
                     LTPLRKTPGGQPSTAEDVLEELSAEHELPRRVLEYRGLAKLKSTYTDKLPELVDAHNR
                     VHTSYHQAVAATGRLSSSDPNLQNIPIRTAEGRRIRQAFVAPEGCRLVAADYSQIELR
                     IMAHLSGDEGLQRAFREDLDVHQATAAEVFETPLDAVTPEQRRSAKAINFGLIYGMSA
                     FGLARQLGIERAAAQEYVQTYFERYPGVKRYMDETRKQAREDGFVTTVLGRRLYLPEI
                     RSRNNQRRQYAERSAINAPMQGTAADIIKLAMIRVAGWLAQGKVDARLIMQVHDELVF
                     EVADGDVDTLVKALDSLMGEAVELSVPLKVQAASGLNWDEAH"
     gene            complement(2064..2681)
                     /gene="yihA"
                     /locus_tag="PLS34_09770"
     CDS             complement(2064..2681)
                     /gene="yihA"
                     /locus_tag="PLS34_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRP87792.1"
                     /translation="MSAYPIAEFLTSANKPSQFVPDQGAEVAFAGRSNAGKSSAINAI
                     LGRRGLARTSKTPGRTQLVNFFSVGEGVRVTDLPGYGYARVPEEVRAHWGKLMDSYFR
                     QRQSLSGLFIVMDVRRPLTDFDQGMLGWAAEAGCPVHILLTKADKLSRGAGSAALHSV
                     RRALGEAATVQLFSALKGTGVEEAREMLDAFLAASGELLNDPETA"
     gene            2784..3383
                     /locus_tag="PLS34_09775"
     CDS             2784..3383
                     /locus_tag="PLS34_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP87793.1"
                     /translation="MRKPFAVAMMAAALGISSTALAEGSAEAGQEKSAVCVACHGVDG
                     NSANPEWPSIAGQHAPYLESQLKAFRDGARQNPLMSPIAMGLSDQDVLDLAAYYAAQR
                     PAAREADPALVDAGERIYKGGVLERGVTACIACHGPTGQGNPLANYPVIAGQHATYAA
                     LALRNYAAGERENQIMQDIAARMSEDDIRAVSEYVQGLR"
     gene            3443..4207
                     /locus_tag="PLS34_09780"
     CDS             3443..4207
                     /locus_tag="PLS34_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRP87794.1"
                     /translation="MRAIAFALVTVILAAACSAEPPAGPAANPPAAQSQAQAPAQAPA
                     AERPAPAAQPAAETSRPAQPPSNARFVAGQHYRVLNPAQPTSVPPGKVEVVDVFWYGC
                     GGCYLMHPYMARWKEAKPAAAEYVRLPATLNPGWQPQARLYFATKALGIEDQTHAAIF
                     NEYHANRNPLNTMELQVKFLSGYGISEQAAREALTSFSVDTELRAADVRARRYQLSFV
                     PAVVVNGKYVVGVEQAGGPDSVLEVINYLVALEAGS"
     gene            complement(4232..>4839)
                     /locus_tag="PLS34_09785"
     CDS             complement(4232..>4839)
                     /locus_tag="PLS34_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP87795.1"
                     /translation="SELWGDAETVMSAYRRGGFQSVTVQLEDAAALEPFKAALADDPR
                     LNVEAQTLRAYFAAQSRQMSMLINVLGYTIGYIMAIGALFGALNTMYAAVSTRAREIA
                     TLRALGFGGGAIVASVLLEALLLAVLGGVLGGGLAWLVFNGYTVSTLGANFSQVAFDF
                     RVTGELLVKGIAWSLAIGFLGGLLPAWRAARQPVTTALRAL"
BASE COUNT          806 a         1718 c         1625 g          690 t
ORIGIN      
        1 cgccgatccc tcgaccagcg agaccaccga ctacgagacc gtgctgaccg agccgcagct
       61 cgacggctgg ctgaaacgtc tgcgcaaggc gcacgagttc gccttcgaca ccgagaccga
      121 cagcctcgac tacatgcgcg cccgcatcgt cggcgtgtcc ttcgccgtcg agccgggatg
      181 tgccgcctac gtgccgctcg cgcaccgcga ccccgatgcg cctgggcagt tgccgctgga
      241 ggacgtgctc gagcgcttgc ggccgctgct ggaggatgcg gacaaggcca agatcggcca
      301 tcacctcaag tacgaccggc acgtgctggc caaccacggc attgcgctcg agggtattcg
      361 cttcgacacc atgctggagt cctacgtgct cgactccacg gcgacgcgcc acgacatgga
      421 ctcggtggcg ctgaagtacc tcggtgtgaa gaccattcac tacgaagacg tggccggcaa
      481 gggtgcgaag cagatcagct tcgaccaggt gccggtggcg accgcggcgc cctacgccgc
      541 cgaggacgcc gacgtcaccc tgcggctgca ccgcgagctg tggccgcggc tggtggcgac
      601 gccgggcctg cagcgcatct acgaggaact cgagcagccg ctggtgccga tcctcggcga
      661 catggagcgc cacggcgtgc tgctcgacac ggcgctgctg cgccgccaga gccgcgaact
      721 cgagaagggc atggcggcgg ccgaggcggc ggccttcgag gccgccggcg gcaagttcaa
      781 catcggctcc gccaagcagc tgcaggagat cctgttcgac cggctcggcc tcacgccgct
      841 gcgcaagacg cccggcgggc aaccgtccac cgcggaagac gtgctcgagg agctcagcgc
      901 cgagcacgag ctgccgcgcc gggtgctgga ataccgcggc ctcgccaagc tgaagtccac
      961 ctacaccgac aagctgcccg agctggtcga cgcacacaac cgcgtgcaca cctcgtacca
     1021 ccaggcggtg gcggccaccg gccggctgtc gtcatccgac cccaacctgc agaacatccc
     1081 catccgcact gccgagggcc gccgcatccg ccaggccttc gtcgcgccgg agggctgccg
     1141 gctggtggcg gcggattatt cccagatcga actcaggatc atggcgcacc tgtccggcga
     1201 cgagggcctg caaagggcct tccgcgagga cctggacgtt caccaggcga ccgcggccga
     1261 ggtgttcgag acgccgctcg acgcggtgac gccggagcag cgccgctcag ccaaggcgat
     1321 caatttcggg ctgatctacg gcatgtcggc cttcgggctg gcccggcagc tgggcatcga
     1381 gcgcgccgcc gcccaggaat acgtgcagac ctatttcgag cgctaccccg gcgtgaagcg
     1441 ctacatggac gagacccgca agcaggcgcg cgaggacggc ttcgtcacta ccgtgctggg
     1501 ccggcgcctg tacctgcccg agattcgttc acgcaacaac cagcggcgcc agtacgccga
     1561 gcgcagcgcc atcaacgcgc ccatgcaggg cacggccgcc gacatcatca agctggcgat
     1621 gatccgcgtg gccggctggc tggcgcaggg caaggtggac gcgcgcctga tcatgcaggt
     1681 gcacgacgag ctggtgttcg aggtcgccga cggtgacgtc gacacgctgg tcaaggcgct
     1741 cgactcactg atgggcgaag ccgtcgaact gtccgtgccg ctcaaggtcc aggccgcctc
     1801 cggcctgaac tgggacgagg cgcactgaat tggggtcagc gaaaaccggg gtcagagaca
     1861 ggtttcgttc gcgaaacctg tctctgaccc cggttttcgc caattcggaa cttttgccgc
     1921 ggtgcggggt cattatccgt gagcgccaat gcgtcgctcg cccgccgtcc tccctgggcg
     1981 ggccagctgc ccggttcccc caagccgggc ccagctctgc cccggggcgc actgccccgg
     2041 ggtttttttt atcttgttga tgttcaggcg gtttccgggt cgttgagcag ctcgcccgag
     2101 gccgccagga aagcatcgag catctcgcgg gcctcctcga caccggtgcc cttgagcgcc
     2161 gagaacagct gcacggtggc cgcctcgccc agcgccctgc gcaccgagtg caacgccgcg
     2221 ctgccggcac cgcgcgacag cttgtcggcc ttggtcagca ggatatgtac cgggcagccg
     2281 gcctccgccg cccagccgag catgccctgg tcgaagtcgg tcagcggccg gcgcacgtcc
     2341 atgacgatga acaggcccga gagcgactgc cgctgccgga aatagctgtc catgagcttg
     2401 ccccagtgcg ctcgcacctc ttccggcacc cgggcgtagc cgtagcccgg caggtcggtc
     2461 accctgacgc cctcgcccac cgagaagaag ttcaccagct gcgtgcggcc cggcgtcttg
     2521 ctggtgcgcg ccagcccgcg ccggccgagg atggcgttga tggcgctgga cttgcccgca
     2581 ttggagcgtc ccgcgaaggc cacctcggcg ccctggtcgg gaacgaactg cgacggcttg
     2641 ttggcgctgg tgagaaactc ggcaatcggg taggcgctca tcgttatgga ctgcaactta
     2701 tggtgttggt gtagaatttc gggttctacc cgtcgggcgc cagcctggca agtcgatggg
     2761 cttatcaccg gagaagaagc gccatgagga agccttttgc cgtcgccatg atggccgccg
     2821 cccttgggat cagcagcacg gccctggccg aggggtcagc agaagccggc caggagaaat
     2881 cggcggtctg cgtcgcctgt cacggcgtgg acggcaacag cgccaacccc gagtggccgt
     2941 cgatcgcggg acagcatgcc ccgtacctcg agtcgcagct caaggccttc cgtgacggcg
     3001 caaggcagaa ccccctgatg tcgcccatcg ccatgggcct gtcggaccag gacgtgctgg
     3061 atctcgcggc ctattacgcg gcacagcggc cggcggcccg cgaagccgac ccggcgctgg
     3121 tcgacgccgg tgagcgcatc tacaagggcg gcgtcctcga gcgcggcgta accgcctgca
     3181 tcgcctgtca cggcccgacg gggcagggca acccgctcgc gaactacccc gtgatcgccg
     3241 gccagcacgc cacctatgcc gccctggcgc tgcgcaacta tgccgcgggc gagcgcgaga
     3301 accagatcat gcaggacatc gccgcgcgca tgtccgagga cgacatccgc gccgtgagcg
     3361 agtacgtgca gggcctgcgc tgagggaact ccgcgccctg cgcgctgcct gaaattctcc
     3421 aagcctcccg aggagacgga ttatgagagc tattgccttc gccctggtca ccgtgatcct
     3481 ggcggccgcc tgcagcgccg agccgccggc cggtcccgcc gcgaacccac cggcggcgca
     3541 atcccaggcg caggctccgg cccaggcacc agcggccgag cgtcccgcac cggctgcgca
     3601 gccggcagcg gaaacctccc ggccggcgca gccgccctct aacgcgcgct tcgtcgccgg
     3661 ccagcactac cgcgtgctga acccggccca gccgaccagc gtcccgcccg gcaaggtcga
     3721 ggtggtggac gtgttctggt acggctgtgg cggctgctac ctgatgcatc cctacatggc
     3781 gcgctggaag gaagccaagc cggcggccgc ggaatacgtg cggctccccg cgacgctgaa
     3841 cccgggctgg cagccgcagg cgcgcctgta ctttgccacc aaggcgctcg gcatcgagga
     3901 ccagacgcac gccgcgatct tcaacgagta ccacgccaac cggaacccgt tgaacaccat
     3961 ggagctgcag gtcaagttcc tgtccggcta cggcatttcc gagcaggctg cacgcgaggc
     4021 gctgaccagc ttcagcgtgg acaccgagct gcgcgccgcc gatgtgcgcg cgcgccgcta
     4081 ccagctgagc ttcgtgccgg cggtggtggt caacggcaag tacgtcgtcg gggtcgagca
     4141 ggccggcggt ccggacagcg tgctcgaggt catcaattac ctggtggcgc tggaagccgg
     4201 cagctgatga ggccaggacc gcccggcgcg tctacagggc gcgcagggcg gtcgtgaccg
     4261 gctggcgggc ggcgcgccag gcgggcagca ggccgccgag aaagccgatc gccagcgacc
     4321 aggcgatgcc cttgaccagc agctcgccgg tgacccggaa gtcgaaggcc acctggctga
     4381 agttggcgcc cagcgtggac accgtgtagc cgttgaacac gagccacgcc aggccgccgc
     4441 ccagcacccc ccccagcacc gccagcagca gcgcctcgag cagcaccgag gccacgatcg
     4501 ccccgccgcc gaagccgagc gcacgcagcg tcgcgatctc gcgggcgcgc gtcgacaccg
     4561 ccgcatacat ggtgttgagc gcgccgaaca gcgcgccgat cgccatgatg tacccgatgg
     4621 tgtagcccag cacgttgatc agcatgctca tctggcgcga ctgcgcggcg aaataggcgc
     4681 gcagggtctg ggcctcgacg ttgaggcgcg gatcatccgc cagcgctgcc ttgaacggct
     4741 cgagcgccgc ggcgtcctcg agctgcacgg tgaccgactg gaaaccgccg cgccggtagg
     4801 ccgacatcac ggtctcggcg tcgccccaca gttccgact
//
LOCUS       DAOMPG010000185         4834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7346_length_4834_cov_3.262607, whole genome shotgun sequence.
ACCESSION   DAOMPG010000185 DAOMPG010000000
VERSION     DAOMPG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7346_length_4834_cov_3.262607"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1107)
                     /locus_tag="PLS34_09790"
     CDS             complement(70..1107)
                     /locus_tag="PLS34_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRP87796.1"
                     /translation="MAEPLIAALRLVPYAIPLSRPWKCARGDTVMRRGWLVRVEDSEG
                     AHGWGETAGLPGAGTETAALAEAALRAAVKEFAGLTPAEAHARLPLHDRPAARCGLEC
                     ALLDLAARRAGQPLYRHLRPEAAGEVRVNAFVGAADEGLGDRLVAARAQGFEVAKLKL
                     ATAPLAEEMPLFFAAMEQLPLGLSLRLDVNRGWSTVEAEATLPRFADLPIEALEEPAH
                     DADGEALARLQSMVSFSIALDESLARWPDEGPLPVRRQILKPMVVGGPSRVFGLALRP
                     RTESIVTSSVDTAVGLWLSAHIGAALGNGLAHGLDTASWLSTLLGPAPTPGGVLRLPD
                     APGLGFEPELP"
     gene            complement(1100..2011)
                     /gene="menA"
                     /locus_tag="PLS34_09795"
     CDS             complement(1100..2011)
                     /gene="menA"
                     /locus_tag="PLS34_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HRP87797.1"
                     /translation="MTAAPAQSMLRYWLSAVRPRTLLVALVPVLVGTMLAWHDVGGIV
                     VSILVVTILAAVFVQVATNLHNDVKDFERGADDPGTRLGPRRATAEGWLSAEQVRRGA
                     TLAFVGAGAAGAWLAIAGGWPVVLIGAASIACGWAYSGGARPIAYSSLGEFFVWLFFG
                     VVAVMGTYYLQTGSFDAIAFAAGALLGMPAAAVLVVNNYRDMDNDRQAGRRTFSVVFG
                     ARASRIEYGLLMLLPFALLPLLALAAAPGWLLALAALPWAVLLVRRFVTQAPGPVFNE
                     LLADTARLQLAFGVLLCAGIFVGGLRG"
     gene            complement(2016..2837)
                     /gene="menB"
                     /locus_tag="PLS34_09800"
     CDS             complement(2016..2837)
                     /gene="menB"
                     /locus_tag="PLS34_09800"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRP87798.1"
                     /translation="MDWTTVSELQLEDVLYQHAEGIARISINRPEVHNAFRPETVMEL
                     QRAFTHAHHDPSIGVIILTGEGGKAFCSGGDQSVRGEQGGYKDATGTQHLNVLDLQMQ
                     IRRLPKPVVAMVAGWAIGGGHVLHLVCDLSIAADNARFGQTGPIVGSFDAGLGAGLLA
                     RTIGMKKAKEIWFLCRQYDAAQALEMGLVNTVVPLDKLEEETVAWCRRMLELSPTALR
                     MLKAAFNADTDGLAGIQELAGNATAMFYMTAEGQEGRNAFLEKRKPDFGRFPRRP"
     gene            2983..4374
                     /locus_tag="PLS34_09805"
     CDS             2983..4374
                     /locus_tag="PLS34_09805"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HRP87799.1"
                     /translation="MDSTPLEAHPRDAFPDELPGQLARALAAAPRQGLLSLSLRARSF
                     PGTPGLPAEGPALWWGRTSENRFRVGAGTAREIETGGTGRFKALAEAHRELRRDWHAA
                     SLDGSPCSRAAFLGFAFSPDFGDAALPNALLSVPRVLVERRGNATVMHFNGAGDEDHD
                     WLLDEWTRIARELFAGAGPDSLAMDPAPHRPPPGALQSEDPDWAARVRHALAAIEQPP
                     LEKLVLTRRVDLPLDAPPSLARILEWLAGRYPDAAVFAVRRPGVSLVGASPERLLGLE
                     HGLVVADALAGSAPRAACDDADQLLGGTLLGDPKELAEHAVVVREILSALEPLCTALV
                     ASHAPSLLRLPNVQHLASHVHGRVKPEVGVFELLERMHPTPAVGGAPRAAALDWLRRH
                     GEADRGWYTGGVGWLDDAGGDVWVALRCATLRGEVASLYAGAGIVAGSDPLQELRETG
                     WKLQAMLEALSVD"
     gene            4367..>4834
                     /locus_tag="PLS34_09810"
     CDS             4367..>4834
                     /locus_tag="PLS34_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRP87800.1"
                     /translation="MTDRDRAVDAGEASFRRAVALLDGLTAAGLESVVASPGSRSTPL
                     ALAAARCTGLRLHVVADERSAAFFALGLVRASGRPAAVIATSGSAPAHWLPAVIEACE
                     DEQPLLLLSADRPPELLGCGANQATEQGRLFDAHAASGAVVVRCAAAAPGTLVA"
BASE COUNT          679 a         1791 c         1709 g          655 t
ORIGIN      
        1 cccatggcgg cacgctcccg cggccgaccg gaacacagtc catggcggca cgctcccgcg
       61 gtcgaatgcc tacggcagct ccggctcgaa gccgagtccc ggcgcatcgg gcagtcgcaa
      121 caccccgccc ggggtcggcg ccgggccgag cagcgtgctc agccagctgg cggtgtcgag
      181 cccgtgcgcc agaccgttgc caagcgccgc gccgatgtgc gccgacagcc acagccccac
      241 cgcggtgtcc acgctgctgg tgacgatgct ctcggtgcgc ggccgcagcg ccaggccgaa
      301 cacccgcgac ggcccgccca ccaccatcgg cttgaggatc tggcggcgca ccggcagcgg
      361 gccctcgtcc ggccagcgcg ccagcgactc gtcgagcgcg atggagaaac tcaccatgga
      421 ctgcaggcgc gccagcgcct cgccatcggc gtcgtgcgcc ggctcttcca gcgcctcgat
      481 cggcaggtcg gcaaagcgcg gcagggtcgc ttcggcctcc accgtgctcc agccgcgatt
      541 cacgtcgagc cgcaggctca ggcccagcgg cagctgctcc atcgccgcga aaaacagcgg
      601 catttcctcg gccagcggcg cggtcgccag cttcagcttg gcaacctcga aaccctgcgc
      661 gcgggccgcc accaggcgat cgccgagacc ttcgtccgcc gcgccgacga aagcattgac
      721 ccgcacctcg ccggccgcct cgggccgcag gtgccgatac agcggctgcc cggcgcggcg
      781 tgcggcgaga tccagcagcg cacattcgag gccgcagcgc gcagcgggac gatcatgcag
      841 cggcagccgc gcgtgcgcct cggccggcgt aagcccggca aactccttga cggccgcacg
      901 cagcgcggcc tccgccagcg ctgcggtttc cgtgcccgcc ccgggaagcc cggcggtctc
      961 gccccagccg tgcgccccct cgctgtcctc gacgcggacg agccagccgc ggcgcataac
     1021 tgtgtcgccg cgcgcacact tccagggccg cgacaggggg atggcatacg gcaccagccg
     1081 caacgcagcg atgagcggct cagccacgca ggcctcccac gaaaattccg gcgcacagca
     1141 gcacgccgaa agccagctgc aatctcgccg tgtcggcgag cagctcgttg aacaccggtc
     1201 cgggggcctg agtcacgaag cgccggacca gcagcaccgc ccagggcagc gcggccagtg
     1261 ccagcagcca gccgggcgcg gcggccagcg ccagcagcgg cagcagcgca aagggcagca
     1321 gcatcagcag gccgtactcg atgcggctgg cgcgcgcgcc gaacaccacc gagaaagtcc
     1381 gccgcccggc ctgccggtcg ttgtccatgt cacggtaatt gttgaccacc agcacggccg
     1441 ctgcgggcat gcccagcagg gcgccggcgg caaaggcgat cgcatcgaag ctgccggtct
     1501 gcaggtagta ggtgcccatg accgccacca cgccgaagaa cagccagacg aaaaactcgc
     1561 cgaggctgga gtaggcgatc ggccgggcgc cgccgctgta ggcccagccg caggcgatcg
     1621 aggccgcgcc gatgagcacc accggccagc cacccgcgat ggcgagccac gcaccggccg
     1681 cgcccgcgcc gacgaaggcc aacgtggcac cgcgccgcac ctgctccgcc gacaaccagc
     1741 cctcggcggt ggcgcgccgc ggcccgaggc gggtgccggg atcgtcggcg ccgcgctcga
     1801 agtccttgac gtcgttgtgc aggttggtcg ccacctgcac gaacaccgcc gcgaggatgg
     1861 tgaccaccag gatcgagacg acgattccgc cgacgtcgtg ccaggcgagc atggtgccga
     1921 ccagcacggg taccagcgcc accagcagcg tgcgcggccg gacggcgctc agccagtagc
     1981 gcagcatgct ctgcgcgggg gcagccgtca cggcgtcagg ggcggcgcgg gaagcggccg
     2041 aaatccggct tgcgcttctc gaggaaggcg ttgcggcctt cctggccttc tgcggtcatg
     2101 tagaacatcg cggtggcatt gccggcgagc tcctggatgc ccgccagccc gtcggtgtcc
     2161 gcattgaacg ccgccttgag catgcgcagc gcggtcggcg acagctcgag catgcggcga
     2221 caccacgcca ccgtttcttc ctcgagcttg tccagcggca caacggtgtt gaccagcccc
     2281 atctccagtg cctgcgcggc atcgtactgg cggcacagga accagatctc cttcgccttc
     2341 ttcatgccga tggtgcgcgc cagcaggccc gcgccgagtc cggcatcgaa gctgccgacg
     2401 atcggtccgg tctggccgaa gcgcgcgttg tcggcggcga tcgacaggtc gcacaccagg
     2461 tgcagcacgt gcccgccgcc gatggcccag ccggccacca tcgccaccac cggcttgggc
     2521 aggcggcgaa tctgcatctg caggtccagc acgttgaggt gctgcgtgcc ggtggcgtcc
     2581 ttgtagccgc cctgctcgcc gcggacgctc tggtcgccgc cggagcagaa cgccttgccg
     2641 ccctcgcccg tcaggatgat cacgccgatc gaggggtcgt gatgcgcatg cgtgaaggca
     2701 cgctgcagct ccatcaccgt ttccggccgg aatgcgttgt gcacctcggg ccggttgatc
     2761 gagattcgcg caatgccctc ggcgtgctgg tagagcacgt cctcgagttg cagttccgag
     2821 acagttgtcc agtccattgc catgtcagcc tgtggggcga aaggcgcaca ttgtacttgg
     2881 ggccggaagt ccgtccacct tccctggcct ggctgcgggg attacgcagg cgggccggcg
     2941 gctgcgtgat acactttcgc ggattgaaac ggccggaaaa ccgtggattc cacacccctc
     3001 gaagcacacc cgcgcgacgc ctttcccgat gagctgccgg ggcagctcgc ccgggcgctt
     3061 gcggctgcgc cccgccaggg attgttgagc ctttcgctgc gcgccaggag cttcccgggc
     3121 acacccggac tgccggccga gggcccggcg ctgtggtggg gccggaccag cgaaaaccgg
     3181 tttcgcgtcg gcgccggcac cgcccgcgag atcgagaccg gcggcaccgg gcgcttcaag
     3241 gcgctggctg aagcccatcg cgagctgcgg cgtgactggc atgccgccag cctcgacggc
     3301 agcccgtgct cgcgcgcggc ctttctcggc ttcgccttca gcccggattt cggcgacgcg
     3361 gcgctgccca acgccctgct gtcggtgccg cgggtgctgg tcgagcgccg cggcaacgcg
     3421 accgtcatgc acttcaacgg cgccggcgac gaggaccatg actggctgct cgacgagtgg
     3481 acccgcatcg cgcgcgaact gttcgccggc gccggcccgg attcgctggc catggacccc
     3541 gcaccgcatc gcccgccgcc gggtgcgctg caatccgagg acccggactg ggccgcccgg
     3601 gtacgccatg cgctggcggc gatcgagcaa ccgccgctgg aaaagctggt gctcacgcgc
     3661 cgggtggact tgccgctcga tgcgccgccc tcgctggcgc gcatcctgga gtggctggcg
     3721 ggccgctacc cggacgccgc ggtgttcgcg gtgcgccggc cgggcgtcag cctggtgggc
     3781 gcgtcgccgg agcggctgct cgggctggag cacggcctgg tggtagccga cgcgctggcc
     3841 ggctccgcgc cgcgcgccgc ctgcgacgat gccgaccagt tgctcggcgg caccctgctg
     3901 ggcgatccca aggagctggc ggagcacgcc gtcgtggtgc gcgagattct cagcgcgctg
     3961 gagcccctgt gcacggcgct ggtcgcctcg cacgcgcctt cgctgctgcg gctgccgaat
     4021 gtgcagcacc tcgccagtca cgtgcacggc cgcgtcaagc cggaggtggg cgtgttcgag
     4081 ctgctggagc gcatgcaccc cacgccggcc gtcggcggcg caccgcgcgc cgcggcactg
     4141 gactggctgc gtcgccacgg cgaggccgac cgcggctggt acaccggcgg cgtcggttgg
     4201 ctggacgatg ccggcggcga cgtctgggtg gcgctgcgtt gcgccaccct gcgcggcgag
     4261 gtggccagcc tgtacgccgg cgccggcatc gtcgcgggtt ccgacccgct acaggaactg
     4321 cgcgagaccg gctggaagct gcaggccatg ctcgaggcgc tgagcgttga ctgatcgtga
     4381 ccgcgcggtg gatgccggcg aggcatcgtt ccggcgcgcg gtcgctttgc tggacggcct
     4441 caccgcggcg ggcctcgagt cggtggtggc gtcgcccggc tcccgctcga cgccgctggc
     4501 gctggccgcg gcccgctgca ccggcctgcg cctgcacgtg gtcgccgacg aacgttccgc
     4561 cgccttcttc gcgctcggac tggtgcgcgc cagcggccgc ccggcggccg tgatcgccac
     4621 ctcgggctcg gcgccggcgc actggctccc ggcagtcatc gaggcctgcg aggacgagca
     4681 gccattgctg cttctcagcg ccgaccggcc gcctgagctg ctcggttgcg gggccaacca
     4741 ggcgaccgag cagggccgcc tgttcgacgc ccatgctgcc agcggcgccg tggtggtgcg
     4801 ctgcgcggcg gcggcgccgg gtacgctggt ggcg
//
LOCUS       DAOMPG010000186         4816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7387_length_4816_cov_4.577400, whole genome shotgun sequence.
ACCESSION   DAOMPG010000186 DAOMPG010000000
VERSION     DAOMPG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_4816_cov_4.577400"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>237)
                     /locus_tag="PLS34_09815"
     CDS             complement(<1..>237)
                     /locus_tag="PLS34_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP87801.1"
                     /translation="LIPSPDYPLWTAATVLNGARAVHYPCRPENGFLPDPEEIGRLVT
                     PRTRALVVINPNNPTGAVYPRPLLEALARLAERHR"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<248..607)
                     /locus_tag="PLS34_09820"
     CDS             complement(<248..607)
                     /locus_tag="PLS34_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRP87802.1"
                     /translation="MTPEIVEPSRALNGVKYEIRGRLARRALELERMGYEIIALNIGN
                     PGLYGFRTPETMRLAMIENLQASEAYCHQKGIFPAREAVVMQQQSRGVTGVTAEDVFI
                     GNGVSELIDLTLRALVND"
     gene            complement(723..4436)
                     /gene="metH"
                     /locus_tag="PLS34_09825"
     CDS             complement(723..4436)
                     /gene="metH"
                     /locus_tag="PLS34_09825"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRP87803.1"
                     /translation="MPEQLQPRRQPDRSRQLAELLSRRILLLDGATGTLMQERKLTEA
                     DFRGERFPASADLKGNYDVLCLTRPDIVAGVHRAYLEAGADVIETNTFSATSIAQGDY
                     GLQDCVVEMNFAAARIARECADAAAARDGRPRFVAGVLGPTNRTASISPDVSDPGKRN
                     VRFDELVAAYREAALALADGGADIIMVETVFDTLNARAALYALEAMFDEEGFRLPVMV
                     SGTITDASGRTLSGQTVEAFWNSVRHARPLVVGFNCALGAEQLRPHIEELSRLADTFV
                     SVHPNAGLPNAFGGYDQDAATMAALIGEFADSGFVNLVGGCCGTRPEHIAALAEAIHG
                     KAPRLVPERPRACRLSGLEPLNIDADSLFVNVGERTNVTGSAKFRKLIEAGDFTGALE
                     VARQQVESGAQIIDVNMDEGMLDSEAAMQTFLDLLATEPDIARVPIMIDSSKWSVIET
                     GLKCLQGKSVVNSISLKEGEEVFLRQAREIRRHGAAVIVMAFDEEGQAETLARRLEIC
                     RRSWRLLTEAAGLPAEDIIFDPNIFAVATGIETHDRYALDFIEATRVIREDIGALVSG
                     GVSNVSFAFRGNDRVREAMHSVFLYHAIRAGMSMGIVNAGQLAIYEDIPGRLRDAVED
                     VVLARRTDATERLLNLAAEFRGGPGARRESEDSGWREWPVLKRLEHALVKGLDEHIVA
                     DTEAARLELGRPLDVIEGPLMPGMNVVGDLFGEGKMFLPQVVKSARVMKKAVAHLVPY
                     LEQEKEADRQPRARIVMATVKGDVHDIGKNIVGVVLQCNGFEVVDLGVMTPCDRILDA
                     ARREQADVIGLSGLITPSLDEMIHVAAEMQRLGFTQPLLIGGATTSPAHTSVKIDPAY
                     QGPVVYVKDASRAVGVVQKLISAEHGPAFVAHVEDEHARRRAQHQRPRGKGPQLSLAE
                     ARDNRFDGGWADYRPPAPVRPGITAFNGFPLEELLPYIDWMPFFNAWEFSGRFPAILE
                     DPTRGEVARALYDDARRMLDDIVREQWLEARGVVGLFPAAGVGDDVRVFTGEDRTATL
                     LTTHHLREQKRKAPGQPNLCLADFVAPQDTGVADWIGGFAVTTGHGADERVARFEAEH
                     DDYRAILLKALADRLAEAFAECLHERVRRELWGYAPDEHLANEALIAEDYRGIRPAPG
                     YPACPDHTEKGTLFQLLGAEARTGIRLTEHYAMLPAAAVSGWYFAHPGARYFQVGRID
                     RDQVEDYAARKGWTIAEAERWLGPILGYERSAA"
     gene            complement(4429..>4816)
                     /locus_tag="PLS34_09830"
     CDS             complement(4429..>4816)
                     /locus_tag="PLS34_09830"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012666536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRP87804.1"
                     /translation="DAGASRAITQFFFDPAVFLRFRDAAAATGIAVPIVPGILPITRF
                     PQIIKFATACGAGIPDWLRERFAGLEDDADTRAMIAASVAIDMVETLRRHGVSDFHFY
                     TLNRAELVYAICHCLLHSPAPGVAYA"
BASE COUNT          745 a         1643 c         1639 g          779 t
ORIGIN      
        1 cggtggcgct cggccaggcg cgccagggcc tcgagcagcg gccggggata gacggcgccg
       61 gtcgggttgt tcgggttgat caccaccagc gcgcgggtgc gcggcgtcac gaggcgtccg
      121 atctcctccg gatccggcag gaagccattc tccggccggc agggatagtg cacggcgcgc
      181 gcgccgttga gcaccgtcgc ggccgtccag agcgggtagt ccgggctcgg gatcagcnnn
      241 nnnnnnngtc gttgaccagt gcgcggagag tcaggtcgat cagctcgctg acgccgttgc
      301 cgatgaacac gtcctcggcc gtcacgccgg tcacgccgcg gctctgctgt tgcatgacca
      361 ccgcttcgcg ggcggggaag atgcccttct ggtggcagta ggcctcactg gcctggaggt
      421 tctcgatcat cgccaggcgc atcgtttcgg gcgtgcgaaa gccgtacagc ccggggttgc
      481 cgatgttcag cgcgatgatc tcgtaaccca tgcgttcgag ctcgagcgcg cgccgcgcca
      541 ggcggccgcg gatctcgtac ttgacgccgt tcagtgcccg gctgggctcg acgatctcgg
      601 gggtcatctg gggtaattcc atcgccacca tgctgcaaag cattaaatca gaaaaccgga
      661 agaaaaacgg ggtcagagac gagttttccg ggaaaactcg tctctgaccc cgtttttctc
      721 tctcaggcgg cggagcgctc gtagccgagg atcggaccca gccagcgctc ggcctcagcg
      781 atcgtccagc ccttgcgtgc ggcgtagtcc tcgacctggt cgcggtcgat gcgcccgacc
      841 tggaagtagc gcgcgcccgg gtgggcgaag taccagccgc tgaccgcggc cgccggcaac
      901 atcgcgtagt gctcggtgag ccggatgccg gtgcgcgctt ctgcacccag cagctggaac
      961 agcgtgcctt tctcggtgtg gtccgggcag gccgggtagc ccggcgcggg ccggatgccg
     1021 cggtagtctt cggcgatcag ggcctcgttg gcgaggtgtt cgtccggcgc gtagccccac
     1081 agctcgcgcc gcacccgctc gtgcaggcac tccgcaaagg cctcggcgag gcggtcggcc
     1141 agcgctttca gcaggatggc gcggtaatcg tcgtgctccg cctcgaaacg cgcgacgcgc
     1201 tcgtcggcac cgtggccggt ggtcacggcg aatccgccaa tccagtccgc cacgccagtg
     1261 tcctgcgggg cgacgaaatc ggccaggcag aggttcggct gcccgggcgc cttgcgtttc
     1321 tgctcgcgca ggtggtgggt ggtcagcagg gtggcagtgc gatcctcccc ggtaaaaacg
     1381 cgaacgtcgt cgccgacgcc ggccgccggg aacaacccga ccacgccgcg cgcctccagc
     1441 cactgctcgc ggacgatatc gtcgagcatc cggcgggcat catcgtacag cgcccgcgca
     1501 acttcgccgc gggtcggatc ctcgaggatg gccggaaacc ggcccgagaa ctcccaggca
     1561 ttgaagaacg gcatccagtc gatgtaaggc agcagttcct cgagcggaaa gccgttgaaa
     1621 gcggtgatgc ccgggcgcac cggcgccggc gggcgatagt ccgcccagcc gccgtcgaag
     1681 cggttgtcgc gcgcctccgc gagactgagc tgcggaccct tgccccgcgg acgctggtgc
     1741 tgcgcccggc gccgcgcatg ctcgtcttcg acgtgcgcca caaaggccgg accatgctct
     1801 gcgctgatca gcttctgcac cacacccacg gcgcgcgacg cgtcctttac gtacaccacc
     1861 ggcccctggt aggccggatc gatcttgacg gaagtgtgcg ccggggacgt cgtcgcgccg
     1921 ccgatcaaca gcggctgcgt gaatcccagt cgctgcatct ctgcggcgac gtgaatcatc
     1981 tcgtccagcg acggggtgat caacccggac aagccgatga catccgcctg ctcgcggcgg
     2041 gccgcatcga gaatccggtc gcagggcgtc atcacgccga ggtccacgac ctcgaagccg
     2101 ttgcattgca gcaccacgcc gacgatgttc ttgccgatgt cgtgcacgtc gccttttacg
     2161 gtcgccatga cgatgcgcgc ccgcggctgg cggtcggctt ccttttcctg ctccaggtaa
     2221 ggcaccaggt gggccacagc tttcttcatg acccgcgccg acttcaccac ctgcggcagg
     2281 aacatcttgc cctcgccgaa caggtcgccg accacgttca tgccgggcat caatggtcct
     2341 tcgattacgt ctagcggccg gccgagttcc agccgcgcgg cctcggtgtc cgccacgatg
     2401 tgctcgtcca ggcccttcac cagcgcgtgc tcgagccgct tcagcaccgg ccactcgcgc
     2461 cagccggagt cctcggactc gcgcctggcg ccgggaccgc cccggaattc cgccgccagg
     2521 ttcaacagtc gttcggtcgc atcggtacga cgggccagga ccacgtcctc gacggcgtcg
     2581 cgaagccgtc ccgggatgtc ctcgtagatc gccagctggc ccgcattgac gatgcccatg
     2641 ctcatgccgg cacggatggc atggtacagg aacaccgagt gcatcgcctc gcgcacgcgg
     2701 tcgttgccgc gaaaggcaaa ggacacgttg gacacgccgc cgctgaccag cgcgccgatg
     2761 tcctcgcgga tgacgcgcgt cgcctcgatg aagtccagcg cgtagcggtc gtgcgtctcg
     2821 atgccggtcg ccacggcgaa gatgttcggg tcgaagatga tgtcttcggc gggcagtccg
     2881 gcggcctcgg tcagcagccg ccaggagcgg cggcagattt ccaggcgtcg cgccagcgtc
     2941 tcggcctggc cctcctcgtc gaaggccatg acgatcacgg ccgcgccgtg gcggcggatc
     3001 tcgcgcgcct ggcgcaggaa gacctcctcg ccctccttca ggctgatcga gttcaccacc
     3061 gacttgccct gcaggcattt cagcccggtc tcgatgaccg accacttgga agagtcgatc
     3121 atgatcggca cgcgggcgat gtccggctcg gtcgcgagca ggtcgaggaa agtctgcatc
     3181 gccgcttcgg agtcgagcat gccctcgtcc atgttgacgt cgatgatctg tgcgccgctc
     3241 tccacctgct ggcgcgcgac ctcgagcgcg ccggtgaaat cacccgcctc gatgagcttg
     3301 cggaacttcg cggagccggt gacgttggtc cgctcgccga cgttgacgaa cagcgagtcc
     3361 gcgtcgatgt tcagcggctc caggccgctc aggcggcagg cgcgcggccg ctccggcacg
     3421 agccgcggcg ccttgccgtg gatcgcctcg gccagtgcgg cgatgtgctc gggccgggtg
     3481 ccgcagcagc cgcccaccag gttgacgaaa ccgctgtcgg cgaactcgcc gatcagcgcg
     3541 gccatggtcg ccgcgtcctg gtcgtagccg ccgaaggcat tcggcaggcc ggcgttcggg
     3601 tgcacgctca cgaaagtgtc ggcgagtcgc gagagctcct cgatgtgcgg ccgcagctgc
     3661 tccgcgccca gtgcacagtt gaagcccacc accagcgggc gcgcgtggcg caccgaattc
     3721 cagaacgcct cgacggtctg gccggagagc gtgcggcccg acgcgtcggt gatggtgccg
     3781 gagaccatca ccgggaggcg gaagccctct tcgtcgaaca tggcctccag cgcgtacagc
     3841 gcggcgcgcg cgttcagcgt gtcgaacacg gtctcgacca tgatgatgtc cgccccgccg
     3901 tcggccagcg caagagcggc ctcgcgatag gccgcgacca gctcgtcgaa gcgcacgttg
     3961 cgcttgccgg ggtcgctgac gtcgggcgag atggacgcag tgcggttggt gggcccgagc
     4021 acaccggcga cgaagcgcgg cctgccgtcc ctggccgctg ccgcatccgc gcattcccgc
     4081 gcgatgcgcg ccgcggcgaa attcatctcc acgacgcagt cctgcaggcc gtaatcgccc
     4141 tgtgcgatcg aggttgcgct gaaggtgttg gtttcgatga cgtcggcgcc ggcctcgaga
     4201 taggcgcgat gcacgccggc tacgatgtcc ggccgcgtca ggcagagcac gtcgtagttg
     4261 cccttcaggt ctgcgcttgc gggaaagcgc tcaccgcgga aatccgcctc ggtcagcttc
     4321 cgttcctgca tcagggtccc ggtcgcgccg tcgagcagca ggatgcggcg cgacagcagc
     4381 tcggccagtt gccggctgcg gtcgggctgg cgacgcggct gcagctgctc aggcatacgc
     4441 cactccaggc gccgggctgt gcagcaggca atgacagatg gcgtacacga gttcggcccg
     4501 gttcaaggtg tagaaatgga agtccgacac gccgtggcgg cgcagggtct cgaccatgtc
     4561 gatggcgacg ctggccgcga tcatcgcgcg cgtgtcggcg tcatcttcca gtcccgcgaa
     4621 gcgttcccgc agccagtcgg gaatgccggc gccgcaggcg gtcgcaaact tgatgatctg
     4681 cggaaagcgg gtgatcggca ggatgccggg cacgatcggc acggcgatcc cggtcgccgc
     4741 ggccgcgtcg cgaaatcgca ggaacacggc cggatcgaaa aagaactgtg tgatggcgcg
     4801 cgaagcgccc gcatcg
//
LOCUS       DAOMPG010000187         4800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7426_length_4800_cov_4.068282, whole genome shotgun sequence.
ACCESSION   DAOMPG010000187 DAOMPG010000000
VERSION     DAOMPG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7426_length_4800_cov_4.068282"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLS34_09835"
     CDS             complement(<1..651)
                     /locus_tag="PLS34_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87805.1"
                     /translation="MIVRPALSDCIRMAAISVGVATIALLFPFALGIVLTGIPLAGIL
                     KLLGILAAIFATVSLVAGCVMYWLFARDAWSLDSQGVSGLDKSRTKRRIGWGQIDAVV
                     LGEAESDPPDVTRLVIQARSSRTEIVARVVGTKLPEIFGEVIKHAGPEHPLANWFRPG
                     ALENVRRELIALPTTGSRTVVIAIWVLVLGWALGFQFWLLPISQALEGMPDCEAANW"
     gene            complement(705..791)
                     /locus_tag="PLS34_09840"
     tRNA            complement(705..791)
                     /locus_tag="PLS34_09840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(755..757),aa:Leu,seq:cag)
     gene            915..3152
                     /gene="rnr"
                     /locus_tag="PLS34_09845"
     CDS             915..3152
                     /gene="rnr"
                     /locus_tag="PLS34_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRP87806.1"
                     /translation="MKHSGRRGGTSVKSRRDQPEQGRHRVAIPSREQVLAALEAAGRP
                     LEPKALFNALRIDDPEVRGALGNRLRAMIRDGQIIANRRGHYCLVGHIGLVTGIVTGH
                     RDGYGFVTPDAGGDDIFLSPREMREVMHGDKVAVRIARTDERGRAEGKLVEVLARNTR
                     EIVGRYVRESGIGFVIPDNRRFSQSVAIPPAGAKGVKPGDIVVVELTEPPSRDTQPIG
                     RVIENLGEAGGPGIETEIAMRAHGLPFRWPEEAVAEAEAWGDKVRPSAKRNREDLREL
                     PLVTIDGADAKDFDDAVWCEPDGSGWRLIVAIADVSTYVPAGGALDLEARDRGTSVYF
                     PRKVIPMLPETLSNGLCSLNPDVDRLCMVCDMRVGSGGKVSSARFYEGLMRSAARLTY
                     DEVAGILVEGDPGLRRRHAKLLPQLEHLHAVWRALAKARARRGAVDFDLPEVAMVFDP
                     KGHVARVEPRHRNDAHRIIEECMIAANVEAARHLEKHKIPTLYRVHAPPEEEKLGALK
                     EFLGAFGLALPTRERLEPKHLSALVNEVAGRPDADLIETVILRSMSAAAYQPDNIGHF
                     GLALERYAHFTSPIRRYPDLLVHRAIRHLARGGKPADFVHGKQEMEGLGRHCSATERR
                     ADEATRDAMDWLKCEFMLERVGEEFDVMVTGVVDFGLFVQIPEFQIDGLVHISSLGGD
                     YYQRDAVHHRLIGEHTGRVFRLSDRLRVRLLRVSLEDKKIDFELVDDEPQVVKGRRGR
                     KARSR"
     gene            3149..3889
                     /gene="rlmB"
                     /locus_tag="PLS34_09850"
     CDS             3149..3889
                     /gene="rlmB"
                     /locus_tag="PLS34_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRP87807.1"
                     /translation="MSAPQLVWGLHAVREALARAPERVLEMWVLGGRVDDRMAEVVAL
                     AREQGITCQQVRRDTLDGMAGGGAHQGVVARLAEAAPLDENDLETLLDRIEGPPLLLV
                     LDGVQDPQNLGACLRTADAAGAHAVVIPRDRAVGLTPAARKAAAGAAETVPLVRVVNL
                     ARCLRSLKARGIWLVGAEAGAPSAFAADLAGPLALVLGGEGGGLRRLTRETCDLLVSL
                     PMKGSVASLNVSVAAGALLYLALARRPG"
     gene            4076..4507
                     /locus_tag="PLS34_09855"
     CDS             4076..4507
                     /locus_tag="PLS34_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87808.1"
                     /translation="MPATTAAAPAEEVSFSVTGEHQPDADRCFFRLEAGYPPEIARRQ
                     IELDLEFEMPSQDDWIVISMYEGLSVEVPDASPVGKPGRGEPHRAEIMHNFLMETFNM
                     GCEQLRARLIVTACDPAPCPELVVDGRANLFPLELVDDSPR"
     gene            4677..>4800
                     /locus_tag="PLS34_09860"
     CDS             4677..>4800
                     /locus_tag="PLS34_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRP87809.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIERYRGMIEGDGGTIHRQED"
BASE COUNT          771 a         1600 c         1716 g          713 t
ORIGIN      
        1 ccagttcgcc gcctcgcaat cgggcatgcc ttccagggcc tgtgatattg gcaacagcca
       61 gaactgaaag ccgagcgccc acccaagcac aaggacccag atggcgatca ccactgtgcg
      121 tgagccggtc gtaggcaagg cgattagctc gcgccttacg ttttccagcg cacccggcct
      181 gaaccagttt gcaagcgggt gctccggtcc cgcgtgcttg ataacttcgc cgaagatctc
      241 agggagtttc gtgccgacca cacgcgccac gatctccgtt ctgctgctcc tggcctggat
      301 caccagccgc gtaacatcgg gtggatcaga ctcagcttcg ccaagcacga ccgcgtcaat
      361 ctgtccccag ccgatgcgcc gcttcgtccg gctcttgtcc aacccggaga cgccctgcga
      421 atcgagagac caggcgtccc gggcgaatag ccagtacatg acgcaccctg cgaccagcga
      481 taccgtagcg aaaattgccg ccaggatccc aagcagcttg aggatgcccg cgagcggaat
      541 gccagtgagc acgatgccca gggcgaacgg gaagagaaga gcgatcgtgg ccacgccgac
      601 cgatattgcg gccatgcgta tgcagtcgga gagggcgggc ctgacgatca tccgggcagc
      661 ataaagcgcc gctagtgacc ctgaaatacc tgtggcgaag tatgtggtgc cgaggagagg
      721 acttgaacct ccacggggtt acccccactg gcacctgaag ccagcgcgtc taccaattcc
      781 gccacctcgg cacgaggcac ggacaggagg accctgcccg aacgggacgc gcagaatacg
      841 gccggcgtgt atccttgtca acgcggccgg gagggttccc gcgccgattt cccaaattgc
      901 ttccaggggc ttgcatgaaa cattccggaa ggcgtggcgg cacgtcggtc aagtcgcgtc
      961 gtgaccagcc cgagcagggg cggcaccgcg tcgccatccc ctcgcgtgag caggtgctgg
     1021 cggcgctcga agccgccggc cggccgctcg agccgaaggc cctgttcaac gcgctgcgca
     1081 tcgacgatcc cgaggtgcgc ggcgcgctgg gcaaccggct gcgggcgatg atccgcgacg
     1141 gccagatcat cgccaaccgg cgcgggcact actgcctggt cggccacatc ggcctggtga
     1201 ccggtatcgt gaccggtcat cgcgacggct acggcttcgt cacgccggat gccggcggcg
     1261 acgacatttt tctctcgccg cgcgagatgc gcgaggtcat gcacggcgac aaggtggcgg
     1321 tgcgcatcgc gcgcaccgac gagcgtggcc gcgcggaggg caagctggtc gaggtgctgg
     1381 cgcgcaacac ccgcgagatc gtgggtcgtt acgtgcgcga gagcggtatc ggtttcgtga
     1441 ttccggacaa ccgccgcttc agccagagcg ttgccatccc gccggccggc gccaaggggg
     1501 tcaagcccgg cgacatcgtg gtggtggagc tcaccgagcc gcccagccgc gatacccagc
     1561 cgatcggccg ggtcatcgag aacctcggcg aggcgggcgg gccgggcatc gagaccgaga
     1621 tcgccatgcg cgcccatggc ttgccgtttc gctggcccga agaagccgtg gccgaggccg
     1681 aggcgtgggg cgacaaggtg cggccgtcgg ccaagcgcaa tcgcgaagac ttgcgcgagc
     1741 tgccgctggt gaccatcgac ggcgccgacg ccaaggattt cgatgacgcg gtgtggtgcg
     1801 agccggacgg ttcgggctgg cgactgatcg tggccatcgc cgacgtgtcg acctacgtgc
     1861 cggcgggcgg cgcgctggac ctggaggcgc gcgaccgcgg cacctcggtg tacttcccgc
     1921 gcaaggtcat cccgatgctg cccgagacgc tgtccaacgg gttgtgctcg ctgaacccgg
     1981 acgtggaccg gctgtgcatg gtgtgcgaca tgcgcgtggg gtcgggcggc aaggtcagca
     2041 gcgcgcgctt ctacgagggc ctgatgcgct cggcggcgcg cctcacctac gacgaggtcg
     2101 ccggcatcct ggtcgagggc gatcccgggc tgcggcgccg ccatgcgaag ctgctgccgc
     2161 agctcgagca cctgcacgcg gtctggcggg cgctggcgaa ggcgcgtgcc cgccgcggcg
     2221 cggtggactt cgacctgccc gaggtggcga tggtgttcga ccccaagggg cacgtggcac
     2281 gcgtcgagcc gcgccatcgc aacgacgccc accggatcat cgaggagtgc atgatcgcgg
     2341 ccaacgtgga ggcggcgcgc cacctcgaga agcacaagat tccgacgctg taccgcgtgc
     2401 acgcgccgcc cgaggaggag aagctcggtg cgctcaagga attcctcggc gccttcggcc
     2461 tggcgctgcc cacgcgcgag cggctggagc cgaagcacct gtccgcgctt gtgaacgagg
     2521 tcgcgggccg gccggacgcc gacctgatcg agacggtgat cctccgttcc atgagcgccg
     2581 cggcctacca gccggacaac atcggccact tcggcctggc gctggagcgc tacgcgcact
     2641 tcacctcgcc gatccgccgc tatcccgacc tgctggtgca tcgcgccatc cgccacctgg
     2701 cgcgcggcgg caagccggcg gacttcgtgc acggcaagca ggaaatggag gggctgggac
     2761 ggcattgctc ggccaccgag cggcgcgccg acgaggccac ccgcgacgcc atggactggc
     2821 tgaagtgcga gttcatgctg gagcgcgtcg gcgaggagtt cgacgtgatg gtcacggggg
     2881 tcgtggattt cggcctgttc gtgcagattc ccgagttcca gatcgacggg ctggtgcaca
     2941 tcagttcgct gggcggcgac tactaccagc gcgacgcggt ccaccaccgg ctcatcggcg
     3001 agcacaccgg gcgggtgttc cgcctcagcg accggctgcg cgtgcgcctt ctgcgcgtca
     3061 gcctggagga caagaagatc gacttcgagc tggtcgatga cgaaccgcag gtggtcaagg
     3121 gccgccgcgg ccgcaaggcg cgctcgcgat gagcgcgccg cagctggtgt ggggcctgca
     3181 tgccgtgcgc gaggcgctgg cgcgcgcccc ggagcgggtg cttgagatgt gggtgctggg
     3241 cggccgggtg gacgaccgca tggcggaagt agtggcgctg gcgcgggagc agggcatcac
     3301 ctgccagcaa gtgcgccgcg acacgctgga cggcatggcc ggcggcggcg cccaccaggg
     3361 cgtggtggcg cggctggccg aggcggcgcc gctggacgag aacgacctcg agacgctgct
     3421 cgaccggatc gaggggccgc ccctgctgct ggtgctcgac ggcgtgcagg acccgcaaaa
     3481 ccttggcgcc tgcctgcgca ccgcggacgc cgccggcgcc cacgccgtgg tcatcccgcg
     3541 ggaccgggcc gtcggcctga ccccggcggc ccgcaaggcg gcggccggcg ccgccgagac
     3601 cgtgccgctg gtgcgcgtgg tcaacctggc gcgctgcctg cgcagcctca aggcgcgcgg
     3661 gatctggctg gtcggcgccg aggccggggc gccgtcggcg tttgcggccg acctggcggg
     3721 gccgctcgcg ctggtgcttg ggggcgaggg cggcgggctg cggcgcctga cccgcgagac
     3781 ctgcgacctg ctggtcagcc tgccgatgaa gggcagcgtg gcgagcctga acgtctcggt
     3841 ggccgccggg gcgctgctct acctggcgct cgcccggcga ccggggtagc cgcagggcgg
     3901 gccacctggc ctggcggatt gcagggcggt tccgcgttaa acgtgctgag ggcaatcgtc
     3961 gtacccgcac aaggaactca gcatgaattc gaatcttcac tctgtcggcg cggcgtgcgc
     4021 ctgtgccttg ctcagcgcgg gttgtgccaa tctgccgagt atgacgcgag gcgaaatgcc
     4081 ggcaacgact gcagctgcac cggccgagga ggtctcgttc agcgtgaccg gcgaacatca
     4141 acccgatgcc gacaggtgct tttttcgcct tgaggcgggc tatccgccgg aaatcgcccg
     4201 gcgccagatc gagctggacc tcgagttcga aatgcccagc caggacgact ggatcgttat
     4261 ttcgatgtac gagggcctga gtgtcgaggt gcctgatgca tcgcccgtcg gcaagcccgg
     4321 ccgcggcgag cctcaccggg ccgagatcat gcacaacttc ctcatggaga ccttcaacat
     4381 gggctgcgag cagctgcgcg ccaggcttat tgtgacggcc tgcgacccgg cgccctgccc
     4441 ggagctggtg gtcgacggac gcgcgaactt gtttccgctc gagctcgtgg acgacagccc
     4501 gcgctaggcg accagcccgg gcgcgggccg gccgagcccc aggctgcggt cccaattgcg
     4561 ccgcggcgtc tgaatccgtt acgatacgcg gcttcgcgcg ggcatggtgc ccgggcgatt
     4621 ccctgcctca ccgccgatgc cgggtgggag gctgactcaa tccgaaggga gatgacatgc
     4681 ggcactacga aatcgtgttc ctggtccacc cggaccagag tgaacaggtc cctgccatga
     4741 tcgagcgcta ccgcgggatg atcgagggcg acgggggcac catccaccgc caggaagact
//
LOCUS       DAOMPG010000188         4713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7592_length_4713_cov_4.045298, whole genome shotgun sequence.
ACCESSION   DAOMPG010000188 DAOMPG010000000
VERSION     DAOMPG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7592_length_4713_cov_4.045298"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLS34_09865"
     CDS             complement(<1..521)
                     /locus_tag="PLS34_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87810.1"
                     /translation="MHLGITAKLFLGVLATSVLVALVMAVTGQLGFQRDFRRYIDAAE
                     ARRSEALAETLAELYTDSGDWTALTGADRRWRALLRAQPRPAEDRGAEPRRGPARGWP
                     RVALYDANDVLVVGNPPEGGEVSRYAVEVDGVTVGWVVRTRAAQVTDAADLRFQRAQL
                     RNLAVATLAAVLFA"
     gene            complement(614..883)
                     /locus_tag="PLS34_09870"
     CDS             complement(614..883)
                     /locus_tag="PLS34_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87811.1"
                     /translation="MASLREQVELLLPDWESWYPSLFDAARDLGVIRARVCDPGSLLL
                     SNRHAGVNSEAARAYREQWSIEEEPGAGGPPRKPAATSPARRRRR"
     gene            complement(983..1834)
                     /locus_tag="PLS34_09875"
     CDS             complement(983..1834)
                     /locus_tag="PLS34_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87812.1"
                     /translation="MHTFNARLLLLFAALAAGGCATLDALAMARTDRLGEPPFVRHFE
                     PHPAPADAVVLGAVALDRVTLETGVVASTAAALAPLAAAMDARLAAMDCCSPGAVPPA
                     DDGAPWVYVGSAGGESAPPEAEQFIEEYEKFPPMVLHVEFASAAWQAELAAQPAATAY
                     LWLRLALVDYPGADEGLFGKKVVLGERHEVKRPFLRAELQPIQVLQVTGALLAPDGRV
                     IAAGAEGIHAVDTPFKAQIFGLQKEIDPGEIEAVIHELRRDDLPGRPLKWEAALDNLV
                     YRLLHGA"
     gene            1942..2679
                     /locus_tag="PLS34_09880"
     CDS             1942..2679
                     /locus_tag="PLS34_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP87813.1"
                     /translation="MDAAPIDARSAREVVFRARGLTKIYRMGEVEVVALRDLDLDLYR
                     GEFVVLLGPSGSGKSTLLNILGGLDSATSGEVWYSGTDLTAANERELTVFRRDHVGFV
                     FQFYNLIPSLTARENVAAVTEIATRPMKPEDALKIVGMGKRLDHFPAQLSGGEQQRVA
                     IARAIAKNPAVLLCDEPTGALDSATGVVVLEALERANRELGTTTVIITHNMVIAEMAD
                     RVIRMADGHIAGSERNTTRKAPRELHW"
     gene            2679..>4713
                     /locus_tag="PLS34_09885"
     CDS             2679..>4713
                     /locus_tag="PLS34_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP87814.1"
                     /translation="MRILDRKLLRDLWHMRGMAVAISLVMIGGIATFVMSRVTYESLR
                     VTQTRYYIDQRFADVFANLVRAPESVAEQFTELPGVNQVETRVVAPVNLEIEGFDDPV
                     TGKILSLPEFGAPRLNVPLVRLGRLPSPGADNEVAVSEDFAAAHGFRPGDRIVAVIKG
                     HRKRLDIVGIALSPEFMYAIPPGALFPDYERYGILWMNRKALATAFDMDGAFNDVSFS
                     LQRGASERALVERVDLVLERYGGLGAYAREDQFSHRFITEELRGLETMATIFPVIFLG
                     VAAFLLNVVISRLVATEREQIGTLKAFGYSTWQVGLHYIKLVLAISACGLLAGIALGL
                     WFARGLGGIYQVFYSFPYLHFELNPVILLQAVTVTTFAVLAGTVLAVRRAAALPPADA
                     MRPEPPPVYRATLLERVGLQRRLAEPSRMIVRHIARKPLKAALTMLGIAAAGGILMVT
                     NFQRDAIGWMLGVQYGLSSREDLTVMFTDPTPRRALYSLRDIEGVEHVEGFRTVPARL
                     VHGHRSQRLALDGLEPGAELLRVLNAALQPVDIPAEGVVLTEYLAREILRIEPGQMLT
                     VEVLEGSRPVLQVPVVGTTRQYLGVNAYMSREALNRLMGEGPAISGARLAVNDERQVA
                     IYRDLRRMPRVAGTVIRDTSIQQFNEMMEETILYFSFITALLGGFIAFGVVYNS"
BASE COUNT          694 a         1635 c         1667 g          717 t
ORIGIN      
        1 gcgaacagca ccgcggccag cgtggccacc gccaggttgc gcagctgggc ccgctggaaa
       61 cgcaggtcgg cggcgtcagt gacttgcgcc gcgcgtgtcc gcaccaccca gcccaccgtc
      121 acgccgtcca cctcgacggc gtaccgcgac acctcgccgc cctcgggcgg gttgccgacc
      181 accagcacgt cgttcgcatc gtagagcgcc acgcgcggcc acccgcgggc cgggccgcgg
      241 cgcggctccg ccccgcggtc ctcggccggc cggggctggg cgcgcagcag cgcgcgccag
      301 cggcggtcgg ccccggtcag cgccgtccag tcgccgctgt cggtgtacag ctcggcgagc
      361 gtctcggcca gcgcctcgct gcggcgcgcc tcggccgcgt cgatgtagcg ccgaaagtca
      421 cgctgaaagc cgagctggcc ggtgacggcc atgaccagcg ccaccagtac gctggtcgcg
      481 agcacgccga gaaacagctt ggcggtgatg ccgaggtgca tgaacctgtc cgtctgcaac
      541 ggccggcaac cagcctaggc cggaattgtg caggaacggc ggagccgggg cacctgggtc
      601 cgcgcggcgg cgctcagcga cggcgccggc gggccggaga agtcgcagcg ggcttgcgcg
      661 gcggcccgcc cgcacccggt tcctcctcga tgctccactg ctcccggtag gcgcgcgccg
      721 cttcgctgtt gacgcccgca tgccggttcg acagcagcag cgagccgggg tcgcacaccc
      781 gtgcgcggat cacgccgagg tcccgcgccg catcaaagag actcgggtac cagctctccc
      841 agtcgggcag cagcaattcg acctgttcgc gaaggctcgc catgggtcga cggtagccca
      901 agggccggga ccggggcaat cgtgcgtcgc ggcggcacga ggccgggcat ggatcgggca
      961 ggcgtggcgc gacgggcgca ggtcaggcgc cgtgcagcag ccggtacacc aggttgtcca
     1021 gcgccgcttc ccacttcagc ggcctgccgg gcaggtcgtc gcgcctcagt tcatggatca
     1081 cggcctcgat ctcgccggga tcgatctctt tctgcaggcc gaagatctgc gccttgaacg
     1141 gcgtgtccac ggcgtgaatg ccctcggccc cggcggcaat gacgcggccg tcgggagcca
     1201 gcagcgcgcc ggtgacctgc agcacctgga tgggttgcag ctcggccctg agaaagggcc
     1261 gcttcacttc gtggcgctcg ccgaggacga ctttcttgcc gaacaggccc tcgtcggcgc
     1321 cgggatagtc caccagcgcc agccggagcc agagatatgc ggtcgccgcc ggctgggctg
     1381 ccagctcagc ctgccaggcg gcagaggcga actcgacgtg cagcaccatg ggcggaaact
     1441 tttcgtattc ctcgatgaac tgctccgcct cgggcggcgc ggactcgccc ccggcactcc
     1501 cgacgtatac ccatggtgcg ccgtcgtccg ccggcggcac cgcgccgggc gagcaacagt
     1561 ccatcgccgc caggcgcgca tccatcgcgg cggcaagcgg ggcgagcgca gcggcggtcg
     1621 aggcgacgac gccggtctcg agggtgacgc gatcgagcgc cacagcgccc agcaccacgg
     1681 cgtccgccgg tgccgggtgt ggctcgaagt gccggacgaa gggcggctcg ccgagccggt
     1741 cggtccgcgc catggcgagc gcgtcgagcg tggcgcagcc gcccgcggcg agggcggcga
     1801 agagcaggag caaccgggcg ttgaaggtat gcatggccgg cgagtgtagc gccgggccgg
     1861 caggccggga ataccagcgc cgtcggcgta tctgggcttt tcccgacggc gttgcgatgg
     1921 ccttgggcgt attcttgcgg catggatgca gccccgattg acgcgcggtc cgcgcgcgag
     1981 gtcgtgttcc gcgctcgcgg gctgaccaag atctatcgca tgggcgaggt ggaggtggtg
     2041 gcgctgcggg acctcgacct cgacctgtac cgcggcgaat tcgtcgtact gctcgggcct
     2101 tccggcagcg gcaagtccac cctgctgaac atcctgggcg gcctcgacag cgcaacttcc
     2161 ggcgaggtgt ggtacagcgg caccgacctt actgctgcga acgagcgcga attgacagta
     2221 ttccgacgcg atcatgtcgg cttcgtgttc cagttctaca atctgattcc cagcctgacg
     2281 gcgcgcgaga acgtcgcggc ggtcaccgag atcgccaccc ggcccatgaa gccggaggac
     2341 gcactgaaga tcgtcgggat gggcaagcgc ctcgatcact tcccggccca gctgtccggc
     2401 ggcgagcagc agcgcgtggc aatcgcgcgc gcgattgcca agaatcccgc ggtgctgctg
     2461 tgcgacgaac cgaccggtgc gctcgattcg gccaccgggg tcgtggtgct cgaggcgctg
     2521 gagcgggcca accgcgaact cggcaccacc acggtgatca tcacgcacaa catggtgatc
     2581 gcggagatgg ccgaccgcgt cattcgcatg gccgacggcc acatcgcggg atccgagcgc
     2641 aacaccaccc gcaaggcgcc gcgcgagctg cactggtgat gcgcatcctg gatcgcaagc
     2701 tgctgcggga cctctggcac atgcgtggca tggcggtggc gatctcgctg gtgatgatcg
     2761 gcggcatcgc gaccttcgtg atgtcgcgcg tcacctacga gtcgttgcgg gtcacccaga
     2821 cgcgctacta catcgaccag cgcttcgctg acgtgttcgc caacctggtg cgggcccccg
     2881 agtcggtggc cgagcagttc accgagctgc cgggcgtcaa ccaggtggag acccgggtgg
     2941 tcgcgccggt gaacctggag atcgagggtt tcgacgaccc ggtgaccggc aagatcctgt
     3001 ccttgcccga gttcggcgcg ccgcgcctca acgtaccgct ggtgcggctc ggccggctgc
     3061 cgtcgccggg ggccgacaac gaggtcgccg ttagcgagga cttcgcggcg gcgcacggct
     3121 tccggccggg cgaccggatc gtggcggtca tcaaggggca tcgcaagcgg ctggacatcg
     3181 tcggcatcgc cctgtcgccg gagttcatgt acgccatccc gccgggcgcg ctgttccccg
     3241 actacgagcg ctacggcatc ctgtggatga accgcaaggc gctggcaacc gccttcgaca
     3301 tggacggcgc cttcaacgac gtcagtttct ccttgcagcg cggtgcctcc gagcgcgccc
     3361 tggtggagcg cgtagacctg gtgctggagc gctacggcgg gctgggcgcc tatgcgcgcg
     3421 aggaccagtt cagccatcgc ttcatcaccg aggagctgcg cgggctggag accatggcca
     3481 ccatctttcc ggtgatcttc ctcggcgttg ccgccttcct gctgaacgtc gtgatcagcc
     3541 gcctggtggc caccgagcgc gagcagatcg ggacgctgaa ggcctttggc tactcgacct
     3601 ggcaggtcgg cctgcattac atcaagctgg tgctcgccat cagcgcctgc ggcctgctgg
     3661 ccggcatcgc gctcgggctg tggttcgcca ggggcctcgg cggcatctac caggtgttct
     3721 acagcttccc ctacctgcat ttcgagctga accccgtgat cctgctgcag gccgtgacgg
     3781 tgaccacctt cgcggtgctg gccggcacgg tgttggccgt gcgccgcgct gcggccctgc
     3841 cgccggccga cgccatgcgg ccggagccgc cgccggtgta ccgggcgacc ctgctggagc
     3901 gggtcgggct gcagcgacgc ctcgcggagc cgtcgcgcat gatcgtgcgg cacatcgcgc
     3961 gcaagccgct caaggcggcg ctgaccatgc tcggcattgc cgcggccggc ggcatcctga
     4021 tggtgaccaa tttccagcgc gacgcgatcg gctggatgct gggcgtgcag tacggactga
     4081 gcagccgcga ggacctgacg gtgatgttca ccgatcccac gccgcggcgc gcactctact
     4141 cgctgcgcga catcgagggc gtcgagcacg tggaaggctt ccgcaccgtg ccggcccgcc
     4201 tggtccatgg ccatcgcagc cagcgcctcg cgctggacgg gctcgagccc ggcgccgagc
     4261 tgctgcgcgt tctcaacgct gccctgcagc cggtggacat ccccgccgag ggcgtcgtgc
     4321 tcaccgagta cctggcgcgc gaaatactgc gcatcgagcc ggggcagatg ctcaccgtcg
     4381 aggtgctcga aggcagccgg cccgtgctgc aggtgccggt ggtcggcacg acgcgccagt
     4441 acctcggcgt gaacgcgtac atgagccgcg aggcgctgaa ccgcctgatg ggcgaggggc
     4501 cggcgatctc cggcgcgcgg ctggcggtga acgacgagcg ccaggtggcg atctatcgtg
     4561 acctgcggcg catgccgcgc gtggcgggca cggtaatccg cgatacctcg atccagcagt
     4621 tcaacgagat gatggaggag accatcctgt acttctcctt catcaccgcg ctgctgggcg
     4681 gcttcatcgc cttcggggtg gtgtacaact ccg
//
LOCUS       DAOMPG010000189         4704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7616_length_4704_cov_3.114433, whole genome shotgun sequence.
ACCESSION   DAOMPG010000189 DAOMPG010000000
VERSION     DAOMPG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_4704_cov_3.114433"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLS34_09890"
     CDS             complement(<1..257)
                     /locus_tag="PLS34_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRP87815.1"
                     /translation="MNFCSACGARVELRVPEGDDRPRHTCTSCGKIHYVNPLIVVGCV
                     PESGSRILLCKRAIEPRYGYWTIPAGFMEIGETMAEGAARET"
     gene            complement(353..916)
                     /locus_tag="PLS34_09895"
     CDS             complement(353..916)
                     /locus_tag="PLS34_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP87816.1"
                     /translation="MTIHRATFPVLLLALSALLSGCSLGQAPAVHAGGTPTTGECPEP
                     RTTQRAPDSYHGLANPLTPSAENIARGRELYVADRSGGSCAACHGVHGGGDGIAGQAL
                     APPPRNFTCAPTMTALSDGQLFWVIESGSGAFHEPARQGAQQVARPGRRESFSAMTSY
                     RQQLSDAEIWQLVLYIRSLAGTAPGKN"
     gene            complement(927..2243)
                     /locus_tag="PLS34_09900"
     CDS             complement(927..2243)
                     /locus_tag="PLS34_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRP87817.1"
                     /translation="MSAGAPATRPANKAHGKCRWFLAALVALGTATPAWTSAETLPAA
                     EALTSRNTLTGDWDGRRAAWAERGVVIGIDHFGDLMAVVDGGEERGSYYAGLVEVGVE
                     FDLATLLGWPDTRAVVLGVGTFGRDPGDGAGSMHAPSNLANVPTGTLLEAWLEREFFD
                     GRLALLGGLYAVDSEFDVKESAGVFMNGGFGTGLELSETGLNGPCVYSATCLGVRARY
                     QPDDARYVQFAVLDGGAGDPDQPRGLQIDLDGDDGLFVIGETGFQRSTEAGRFLRAAL
                     GAWHYTTEFDNIVQPGPPTPLRTSDGTYGVYALLEGSLYGEPGQAVQGMSGFVRIGFA
                     DQKVNPIGRYAGAGLVYTGLLPARDEDVLGLGISVAFLGQDFRDTQALAGTPVQSREL
                     VFELAYWMPVLPWFNLQLDAQYISNPAADPALANATLFGLRYQVTF"
     gene            complement(2383..2469)
                     /locus_tag="PLS34_09905"
     tRNA            complement(2383..2469)
                     /locus_tag="PLS34_09905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2433..2435),aa:Leu,seq:caa)
     gene            2595..3200
                     /locus_tag="PLS34_09910"
     CDS             2595..3200
                     /locus_tag="PLS34_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRP87818.1"
                     /translation="MAGFADCKPDGATLERARGGDMAAHEALYGSYGRPVYTLAMRIL
                     SDPAMAGEIVQETCIEVIRHIGSFRGDAAFGGWVKRIATNKCLAYLRSAWHRKARGPG
                     CGDEADDMDWHTGDAPDPATTVAHQDGLERALAELPPTARAVVWLYDVEGYSHHEIAS
                     LMGKTVSFSKSRLARAHEQLRATLGPDYQEVSTCMPQPNNC"
     gene            3173..3814
                     /locus_tag="PLS34_09915"
     CDS             3173..3814
                     /locus_tag="PLS34_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87819.1"
                     /translation="MHATTEQLLSLRDGRPVDAEVVAHADECVECQQALRGMERLRAR
                     LRALPELDPPRDLWRTLPLDAPRVPERRAQTPWHWPVGAGIAAAAALAIALVLNVRQA
                     PDDGPAPGTTTDLVAATPAPMSSVNGVTTAELLETSRQLETALRVMPAAPQVTRASTA
                     LTIAELQDRIFQVDTMLSEPELDPASEQELWRQRVRLMDTLMQVRYAQLAELR"
     gene            3855..>4704
                     /locus_tag="PLS34_09920"
     CDS             3855..>4704
                     /locus_tag="PLS34_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRP87820.1"
                     /translation="MNTRLIGIITSLAMAAVLAAPAAMAQPAPESPPAPPAASEADRA
                     AAEARLRAAEERLKAAAREIAELSMSVVGDLDLPGLVRFDGRGLRGRTMLGVNIAPVG
                     GADAREEGVLIRGVTPGAPAETAGLRSGDVLLSLGELQLDWSGNSSPVEKLVERVREL
                     EPGTTTELRYRRDGEVASANVEARSWGQVTGIHLDSEWLRGLIPPDAPRAPHFARRFM
                     LDAWGDMELVSLTPGLGDYFKSEEGVLVVRAPSDDTLGLQDGDVILDIAGRKPADPGH
                     VVRILRS"
BASE COUNT          731 a         1587 c         1681 g          705 t
ORIGIN      
        1 gtctcgcggg ccgcgccctc cgccatggtc tcgccgatct ccatgaagcc cgcgggaatc
       61 gtccagtagc cgtagcgcgg ctcgatggca cgcttgcaca ggagaatccg gctgccggac
      121 tcgggcacgc agccgaccac gatcagcggg ttgacgtaat gaatcttgcc acacgatgta
      181 caggtgtggc ggggccggtc gtcaccctcg ggaacgcgga gttcgacgcg tgcgccgcaa
      241 gcgctgcaga agttcataag catgccggtt gccggagtcg gcgttattct gcctgccgga
      301 cgcggtcgct gccagcgcac tggcctcgag ccagcgtcgt cgttggcaca gctcagttct
      361 tgcccggcgc agtcccggcc agcgaccgga tgtagagcac gagttgccag atctcggcat
      421 cgctgagctg ctgccggtag ctggtcatgg cgctaaagct ttcacgccgg ccgggacggg
      481 cgacctgttg cgcgccctgg cgggccggct cgtggaacgc ccccgagccg ctctcgatca
      541 cccagaacaa ctgcccgtcg ctcagcgcgg tcatcgtcgg ggcacaggtg aagttcctgg
      601 gcggcggcgc cagcgcctgg ccggctatcc cgtcgccacc accgtgcacg ccgtggcagg
      661 ccgcacacga gccgccgctg cggtcggcca cgtacagctc gcggccacgc gcgatgttct
      721 cggcagaggg cgtgagcggg ttcgccagcc cgtggtagct gtcgggtgcg cgctgcgtgg
      781 tgcgcggctc cgggcactcg ccggtggtcg gcgttccgcc ggcgtgaacc gcgggggcct
      841 ggccgagcga gcagcctgag agcagcgccg acagcgccag cagcaggacg gggaaggtgg
      901 cacgatgaat ggtcatggca ggcacctcag aacgtgacct ggtagcgcag gccgaacagc
      961 gttgcgttgg cgagcgcggg atcggccgca gggttgctga tgtactgggc atccagctgc
     1021 aggttgaacc agggcagcac cggcatccag tacgcgagtt cgaagaccag ctcccggctc
     1081 tggaccggcg tgccggcgag ggcctgggtg tcccggaaat cctggccgag aaaagccacc
     1141 gagatgccga ggccgagcac gtcctcgtcg cgggccggca acagcccggt gtagaccagc
     1201 ccggcccccg cgtagcgtcc gatgggattc accttctggt cggcgaagcc gatgcgcaca
     1261 aagccgctca tcccctggac ggcctggccc ggctcgccgt acaggctgcc ctcgagcaag
     1321 gcataaacgc cgtacgtccc gtcactggtg cgcaacggtg tcggcggccc gggttgcacg
     1381 atgttgtcga actcggtcgt gtaatgccag gcgccgagtg ccgcccgcag gaaacggccc
     1441 gcctcggtgc tgcgctggaa tcccgtttca ccgatcacga acagcccgtc gtcgccgtcc
     1501 aggtcgatct gcagaccacg tggctggtcc gggtcgcccg cgccgccgtc cagtacggcg
     1561 aactgcacat agcgcgcatc gtccggctgg tagcgggcgc gcacgccgag gcatgtagca
     1621 gaatacacgc acggtccgtt gagcccggtc tcggacagct ccagtccggt gccgaagccg
     1681 ccgttcatga atacgcccgc gctttccttg acatcgaact cgctgtcgac ggcgtagagc
     1741 ccgcccagca aggcgagccg gccgtcgaag aattcgcgct ccagccaggc ctcgagcagc
     1801 gtgccggtgg gcacgttcgc caggttgctc ggtgcgtgca tcgacccggc gccatcgccg
     1861 ggatcgcgac cgaaggtccc gacacccagc accaccgccc gcgtgtccgg ccagcccagc
     1921 aaggtcgcca ggtcgaactc gacgccgacc tcgaccaggc cggcgtaata gctgccgcgc
     1981 tcctcgccgc cgtccacgac ggccatgaga tcgccgaagt gatcgatgcc gatcaccacg
     2041 ccgcgctcgg cccaggcagc gcgtcgcccg tcccagtcgc ccgtcagcgt gttgcggctg
     2101 gtgagtgcct ccgccgcagg caatgtttcg gccgaggtcc aggccggggt cgccgtgccg
     2161 agcgcaacga gcgccgcgag gaaccaacgg catttgccgt gtgccttgtt ggcgggtcgg
     2221 gtcgcggggg ctcctgcaga catgaaacca gctccttcca gttcccttgc ttacccgaat
     2281 gcgaacgatt cgcgtttcgg actatagggt ggcgcgaagc ggggcggaat aagtaattgg
     2341 cgcagcgcat cattcgctgt gatgcgacgt gtcggcgagg cttggtgccc ggggcgagag
     2401 tcgaactcgc acgatgtcgc catcggcgga ttttgagtcc gctgcgtcta ccagttccgc
     2461 cacccgggcc gaggaggggc gcggcccgcc gggccgcgag cgcgaagctt accattggac
     2521 ccgcggcctg gggcagtgcg acgttcggcc cggctggcgc atcttggagg tgaggcgaga
     2581 ttcaaggggg gcgcgtggcg ggattcgcgg actgcaagcc ggatggggcc acactggaac
     2641 gtgcgcgcgg cggggacatg gcggcccacg aggcgctgta cggcagctac ggccggccgg
     2701 tctacacgct ggccatgcgc atcctgtctg atcccgccat ggccggggag atcgtgcagg
     2761 agacctgcat cgaggtcatc cggcacatcg gcagcttccg tggcgacgcg gccttcggcg
     2821 gctgggtcaa gcggatcgcg accaacaagt gtctcgccta cctgcgcagc gcgtggcacc
     2881 ggaaggcacg gggcccgggc tgcggagacg aggcggatga catggactgg cacaccgggg
     2941 atgcccccga cccggcgacc acggtggccc accaggacgg tctcgagcgg gcgctggccg
     3001 aactgccgcc gacggcgcgc gccgtggtct ggctgtacga cgtcgagggc tacagccacc
     3061 acgagatcgc ttcgctgatg ggcaagacgg tgagtttctc gaagtcccgg ctggcacgcg
     3121 cgcacgagca gctgcgcgcc acgctggggc cggattacca ggaggtgagc acatgcatgc
     3181 cacaaccgaa caactgctga gcctgcgcga cgggcgcccg gtggatgccg aggtcgtcgc
     3241 acacgctgac gagtgcgtcg aatgccagca ggcgctgcgt ggcatggagc ggctgcgcgc
     3301 ccggctgcgt gccttgccgg aactcgatcc gccgcgcgac ttgtggcgca ccctcccgct
     3361 ggatgcgcct cgagtgcccg agcgccgggc acaaacgccc tggcactggc cggtcggggc
     3421 cggcattgcg gccgcagcag cgctggcgat cgcgctcgtg ctgaatgtgc gccaggcgcc
     3481 ggatgacggc ccggccccgg gcacgaccac cgacctcgtc gccgccacgc ctgcgccgat
     3541 gagttcggtc aacggcgtga cgaccgcgga gctgctcgag acgtcccgcc agctcgagac
     3601 cgcgttgcgc gtcatgcccg ccgcgccgca ggtcacccgc gccagtactg ctttgacgat
     3661 tgccgagctg caggaccgga ttttccaggt ggacacgatg ctcagcgagc ccgagctgga
     3721 tcccgccagc gagcaggaac tctggcggca gcgcgtccgg ctgatggaca ccctcatgca
     3781 ggttcgctac gcacagctcg ccgagttgcg ctgaggcgcg ccagcccttt tttgaatcac
     3841 tggaggcaag tgcaatgaac accaggctga tcggaatcat cacgtcgctc gcgatggccg
     3901 ccgtgctggc ggccccggca gcgatggcgc aacccgcgcc cgagtcgccg ccggcgccgc
     3961 ccgcagcaag cgaagcagac cgggcggcgg ccgaggcccg gctgcgcgcg gcggaagaac
     4021 ggctcaaggc ggccgcgcgc gagatcgcgg aactgagcat gagcgtggtc ggggatctcg
     4081 atctccccgg cctggtcaga tttgatggcc gcggcttgcg tgggcggacc atgctgggcg
     4141 tcaatattgc gccggtcggc ggcgccgatg ctcgcgagga aggtgtgctg atccggggcg
     4201 tcacgccggg tgcgccggcc gagacggccg gcctgcggag cggtgatgtg ttgctatcgt
     4261 tgggtgagct ccagctcgac tggagcggca acagttcgcc ggtggaaaag ctggtcgagc
     4321 gggtgagaga gctcgagccg ggcacgacga cggagttgcg ctaccggcgt gacggcgagg
     4381 tggcgagtgc gaatgtcgag gcgcggtctt ggggccaggt aaccgggatt cacctggact
     4441 cagagtggct ccgcggcctg atccctccgg atgcgccgcg cgcgccgcac ttcgcgcgcc
     4501 gcttcatgct cgacgcctgg ggggacatgg agctcgtgtc gctcacgccg ggactcggcg
     4561 attacttcaa gtccgaggag ggcgtgctgg tggtccgcgc gccgtccgac gacaccctcg
     4621 gcctgcagga tggcgacgtc atcctcgaca tcgccgggcg caagccggcc gacccgggcc
     4681 acgtggtgcg catcctgcgc tcct
//
LOCUS       DAOMPG010000190         4693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7651_length_4693_cov_4.099396, whole genome shotgun sequence.
ACCESSION   DAOMPG010000190 DAOMPG010000000
VERSION     DAOMPG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7651_length_4693_cov_4.099396"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1220)
                     /locus_tag="PLS34_09925"
     CDS             complement(99..1220)
                     /locus_tag="PLS34_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRP87821.1"
                     /translation="MDEQQHENQRLREQLETLTDQARRNEEIFRRSHERYMNLLQATS
                     LRGLFDAMVFGLAEAYELDAVSLVIQDMRHEVRQLLQAEDPHAVPPHDVLFVETLQGL
                     APQYNNLWQPWLGAWSASDHSLIFPGQGGLCSCALLPLRRNGELIGCLNFGSCDVERF
                     QRSHAVDFLKHLSDIAAVCLENAVNRSRLVRSGITDVLTGLYNRRYLQHRLTDELARA
                     RRDGRPLACAMLDVDRFKPINDRWGHPAGDKVLAQIAHVIREECRDSDIPVRYGGEEF
                     AVVMPGATLADALAAAERLRCEVATLAYQPASAARHALTVSIGVAALGADASGSDEAL
                     AEGLLHRADLALYEAKRQGRNRVVALEADGEMMPAGQPA"
     gene            complement(1236..2675)
                     /locus_tag="PLS34_09930"
     CDS             complement(1236..2675)
                     /locus_tag="PLS34_09930"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRP87822.1"
                     /translation="MAAEPTDIAEQQQVARMRRLFDAQRTAFMAAPMPDYAARRADLK
                     RLKEALIANRERFVAAVSADFGGRAGPETMAEILTVVHHINYCLRRLKRWMKPQRRGT
                     SLLMATTRAMVYQQPLGVVGVVVPWNYPIALSAGPLVFALAAGNRVMVKMSETTPRTA
                     ELMRHVLSEAFEERQVAVVLGETETAREFTRLPFDHLLFTGGSDIGRHVMREAAANLT
                     PVTLELGGKSPTLISADVPMELAAERICFGKALNAGQTCVAPDYVLCPHGRIDEFVDA
                     FGTVLGRMYPGLVRNSDYSAVVNERHFRRIRGYIDDARAKGAEVVELNPGGLSPEPGS
                     RKLPLYLVKNATPQMRVMQEEVFGPVLPIVGYNHLQEAIDFINARPRPLALNLFDFNA
                     ERRRRVLEETHAGGVCINDAVSQFIAEDLPFGGVGESGMGHYHGYEGFLTFSHQKAVF
                     QRPRLNTGKVLYAPHGGWLQRLLYRLYLR"
     gene            2785..3870
                     /locus_tag="PLS34_09935"
     CDS             2785..3870
                     /locus_tag="PLS34_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP87823.1"
                     /translation="MLANPMRRLKKLSPWRRRWRLLAAALLVAALAVSASCAGPVPRH
                     QGPPSDHFDGRRFFHEPRVNKGFWTFLRWRFTRAPTGGWEQDLAPPGAPPPPARVPGD
                     AMQVTFVNHATVLIQTRGLNLLTDPIWSERASPFRSLGPQRHLAPGVDFDDLPTIDAV
                     LLSHNHYDHMDLPTLQRLAERDDPLFLVPLANCAYFRDIPTGRCVELDWWETSELLAG
                     VRIHAVPAVHWSRRGLWDTNRALWSGWVVEGARRVYFAGDTGYGKHFAEIRARLGPPD
                     LAILPIGAYLPRWFMAAQHIDPAEAILATDALGAGVSMAMHHACFELADDGQWQARDE
                     LEALLLDSKDREEAFWVPAHGEARRWN"
     gene            4063..4632
                     /locus_tag="PLS34_09940"
     CDS             4063..4632
                     /locus_tag="PLS34_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP87824.1"
                     /translation="MAAGWPRRALAQRLGLLPQGTEDPFPATVMEEALIGRHPHLGFW
                     QWESPEDLAIARQALADVDLDGLEERLLDTLSGGERRRLAVATLLVQNPAVWLVDEPT
                     NHLDPQHRLGVMRLLAARAGDGRAMVVTLHDVNLAAGWCDRCLLLFGDGSWELGPAAE
                     VLNEAAISRLYRMPVRRVEFEDRALFLPL"
BASE COUNT          679 a         1718 c         1593 g          703 t
ORIGIN      
        1 ctgaccccga tcaggcggac gcacctgatc gggtgaagat gcgcgcaggg aagcctcaag
       61 gccgtttccg acagatcgga aacggctttc gcgtgccgtc aggcgggctg tcccgccggc
      121 atcatctcgc cgtccgcctc cagcgcgacc acccggttgc gcccctggcg cttcgcctcg
      181 tagagcgcga ggtcggcgcg gtgcagcagc ccctcggcca gggcctcgtc gctgcccgag
      241 gcatcggcgc cgagcgcggc cacgccgatg ctgaccgtca gcgcgtggcg tgcggccgaa
      301 gcgggctgat aggcaagcgt ggccacctcg caccgcagcc gctcggcggc ggccagcgcg
      361 tcggcgagcg tcgcccccgg catcaccacc gcgaactcct ccccgccgta gcgcaccggg
      421 atgtcgctgt cgcggcactc ctcgcggatg acgtgcgcga tctgcgccag taccttgtca
      481 cccgccgggt ggccccagcg gtcgttgatc ggcttgaaac gatcgacgtc gagcatggcg
      541 caggccagcg gccgtccatc gcgccgtgcg cgcgccagct cgtcggtcag ccggtgctgc
      601 aggtagcggc ggttgtacag gccggtcagc acgtcggtga tgccactgcg caccagccgg
      661 ctgcggttga cggcgttctc caggcagacc gccgcaatgt ccgacaagtg cttgaggaaa
      721 tcgaccgcat ggctgcgctg gaatcgctcc acatcgcagc tgccgaagtt caggcagccg
      781 atcagctccc cgttgcggcg cagcggcagc agggcgcagc tgcagaggcc gccctgcccc
      841 ggaaaaatca gcgagtgatc ggacgcggac cacgcaccga gccagggctg ccagaggttg
      901 ttgtactgcg gcgcgaggcc ctgcagcgtc tcgacgaaca gcacgtcgtg gggcggcacc
      961 gcgtgggggt cctcggcctg cagcagctgg cgcacctcgt ggcgcatgtc ctggatcacc
     1021 agcgacacgg cgtccagctc gtaagcctcc gccagcccga acaccatggc gtcgaacagc
     1081 ccgcgcagcg aggtcgcctg gaggaggttc atgtagcgct cgtgcgagcg ccggaaaatc
     1141 tcctcgttgc gccttgcctg gtcggtcagc gtctcgagct gctcgcgcag gcgctggttt
     1201 tcgtgctgct gttcatccat gcggactgac gggtctcagc gcaggtacag gcgatacagc
     1261 aggcgctgca gccagccgcc gtgcggcgca tacagcacct tgccggtgtt gaggcgcggg
     1321 cgctggaaca cggccttctg gtgcgaaaag gtcaggaagc cctcgtagcc gtggtagtgg
     1381 cccatgccgg actcgccgac gccgccgaag ggcaggtcct cggcaatgaa ctggctgacc
     1441 gcatcgttga tgcacactcc gccggcgtgc gtctcctcga gcacgcgccg gcgccgctcg
     1501 gcgttgaagt cgaacaggtt cagcgccagc ggccgcggcc gcgcattgat gaagtcgatg
     1561 gcttcctgca ggtggttgta gccgacgatc ggcagcaccg ggccgaacac ctcttcctgc
     1621 atgacgcgca tctgcggcgt ggcgttcttc accaggtaca gcggcagctt gcggctgccc
     1681 ggctcgggcg acagcccgcc gggattgagc tccacgacct cggcgccctt ggcgcgcgcg
     1741 tcgtcgatgt agccccggat gcggcggaaa tggcgctcgt tcaccaccgc gctgtaatcg
     1801 ctgttgcgca ccagccccgg gtacatccgg ccgagcaccg tgccgaaggc gtccacgaac
     1861 tcgtcgatgc gcccgtgcgg gcacagcacg tagtccggcg ccacgcaggt ctggccggcg
     1921 ttgagcgcct tgccgaagca gatgcgctcc gcggcgagtt ccatcggcac gtccgcggag
     1981 atcagcgtcg gcgacttgcc gccgagctcg agcgtcaccg gcgtcaggtt ggcggcggcc
     2041 tcgcgcatca cgtggcgacc gatgtccgag ccgcccgtga acagcaggtg gtcgaagggc
     2101 aggcgggtga actcccgggc ggtttccgtc tcgcccagca ccaccgccac ctggcgttcc
     2161 tcgaaggcct cggacaacac gtggcgcatc agctcggcgg tgcgcggcgt ggtctcggac
     2221 atcttgacca tgacccggtt gccggccgcc agcgcgaaca ccagcggccc ggcggacagc
     2281 gcaatcgggt agttccacgg caccaccacc ccgaccacgc cgagcggctg ctggtagacc
     2341 atcgccctgg tggtggccat caagaggctg gtaccgcggc gctgcggctt catccagcgc
     2401 ttcagccgcc gcaggcagta gttgatgtgg tgcaccacgg tgagaatctc ggccatggtc
     2461 tccggcccgg cgcggccgcc gaaatccgcg gacaccgcgg cgacgaaacg ctcacggttg
     2521 gcgatcagcg cctccttgag gcgcttcagg tcggcgcgcc gcgccgcata gtccggcatg
     2581 ggcgcggcca tgaaggcggt ccgctgggca tcgaacagcc gtcgcatccg cgcgacctgc
     2641 tgctgctcgg caatgtcggt aggctctgcg gccatggctc catgcctcca atcgggtgtc
     2701 acccatgaaa tgtaacctgt tgcagtgcat cgatgccatg tcgggcacca gaaaccttgc
     2761 cccgcccggc cccggaagcc ggttatgctt gccaacccca tgcgccggct gaaaaaactc
     2821 tccccttggc gccgtcgctg gcgcctgctc gccgcggccc tgctggtcgc ggcgctggcg
     2881 gtgtccgcca gttgcgccgg accggtgccg cgccaccagg gtccgccgag cgaccatttc
     2941 gacggccggc gcttcttcca cgagccacgg gtcaacaagg gcttctggac cttcctccgc
     3001 tggcgcttca cgcgcgcccc gaccggcggc tgggagcagg acctggcgcc gccgggggcg
     3061 ccgcccccgc ccgcccgcgt gccgggcgac gccatgcagg tcaccttcgt caaccacgcc
     3121 accgtgctga tccagacgcg cggcctcaac ctgctgaccg acccgatctg gtcggagcgc
     3181 gccagtccct tcaggtcgct cggcccgcag cgccacctcg cgcccggcgt cgacttcgac
     3241 gacctgccga cgatcgacgc cgtgctcctc agccacaacc actacgacca catggacctg
     3301 cccaccctgc aacggctggc cgagcgcgac gaccccctgt tcctggtccc gctcgccaac
     3361 tgcgcgtact tccgcgacat cccgaccggc cgttgcgtgg aactcgactg gtgggaaacc
     3421 agcgagctgc tggcgggtgt gcgcatccac gccgtgccgg cggtgcactg gtcgcgccgc
     3481 ggcctgtggg acaccaaccg cgcgctctgg tccggctggg tcgtggaagg tgcgcggcgc
     3541 gtctacttcg ccggcgacac cggctacggc aagcacttcg ccgagatccg cgcgcgcctc
     3601 ggaccaccgg acctcgccat cctgccgatc ggcgcctacc tgccccgctg gttcatggcg
     3661 gcccagcaca tcgaccccgc cgaggccatc ctcgccaccg acgcgctcgg cgccggcgtg
     3721 tccatggcca tgcaccatgc ctgcttcgag cttgccgacg acggccagtg gcaggcccgc
     3781 gacgagctcg aggcgctgct gcttgacagc aaggaccgcg aggaggcttt ctgggtgccc
     3841 gcccatgggg aagcccgccg ctggaactga cgcacgcgat gcccgacacc gaaacacccc
     3901 tgctcgaggc tcgcgggctc accatcaccg cgggtcggcg ccggctggtc gagagtctcg
     3961 acctggcgct catgccgggc gaggcgctcg ccgtgctcgg cccgaacggc gtcggcaaga
     4021 cgcttaccct gcagaccctg gccgggctgc gcaaaccggc cggtggcggc cgggtggccg
     4081 cgccgcgcgc tggcgcagcg gctcggcctg ctgccccagg gcaccgagga cccgttcccc
     4141 gccacggtga tggaggaagc gctgatcggc cgccatccgc acctgggctt ctggcagtgg
     4201 gaaagtcccg aggacctcgc cattgcccgc caggccctcg ccgacgtgga cctcgacggg
     4261 ctggaagaac gactgctgga cacgctctcc ggcggcgagc gcaggcgcct cgccgtcgcc
     4321 accctgctgg tgcagaaccc cgcggtgtgg ctggtggacg aaccgaccaa ccacctcgac
     4381 ccgcagcacc gcctcggcgt gatgcggctg ctggccgccc gcgccggcga cggccgcgcc
     4441 atggtggtga cgctgcacga cgtcaacctg gcggcgggct ggtgcgaccg ttgcctgctg
     4501 ttgttcggcg acggcagctg ggagctgggc cccgccgccg aggtgctgaa cgaggcggcc
     4561 atctcgcgcc tgtaccggat gccggtgcgg cgcgtcgagt tcgaggaccg cgcgctgttc
     4621 ctgcccctct gaatcggggt cagacaccaa tttccagtcg accgcgggcg cgcggggcct
     4681 tcggttccgg tcg
//
LOCUS       DAOMPG010000191         4649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7751_length_4649_cov_4.614279, whole genome shotgun sequence.
ACCESSION   DAOMPG010000191 DAOMPG010000000
VERSION     DAOMPG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7751_length_4649_cov_4.614279"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="PLS34_09945"
     CDS             <1..2067
                     /locus_tag="PLS34_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP87825.1"
                     /translation="RDELTRHAANDGVWDFDVETNTIRFSPRWKVMMGYGEDEPDEDM
                     PDWRQLVHPEDMVRVQSKIRDHLEGKSELFESVHRMRHRSGDWRWVISRAKAMVDDDG
                     RMLRLVGVELDITDQKLYEEALFREKQNAQITLQSIGDGVITTDSDCRIEYLNPVAQE
                     LTGWATEDAAGRSVDEIFRSFHEETCEPMENPLSVAIRRDRLYKSMRPTLLIRRDGNE
                     LYVESCAAPIRNDMGQVIGGVLVFHDVSEARELNRRLSYHASHDMLTGLVNRREFEAR
                     LERALKMGRAREAQYALCYMDLDQFKIINDSCGHMAGDALLGQLGALLKSKIRWRDTL
                     ARLGGDEFGVLLENCTLDEAIETAEALLKAVQDFRFVWEDRSFRLGASIGVVPIVPET
                     ASVAELLTAADSACAAAKESGRNRVQTWQENDIDLMRRRREMQWAARINNALEESRFE
                     LFRQPIMPLHDQSSGAHYELLLRMRDEQGQIIAPDVFINAAERYGLTPSIDRWVITNA
                     FRWLVSEADERERLAMCSINLSGQSLCDDKFLPFVIEQFERSGLDASKICFEITETAA
                     IASYSQANRFINSLKELGCRFALDDFGTGLSSFGYLKHFPVDFLKIDGSFVKEILHDP
                     IDREMVRSINEIGHLTGKQTIAEFVENKELITMLRGMGVDYAQGYGVGEPRRIMDPEF
                     AVREIR"
     gene            2116..2658
                     /locus_tag="PLS34_09950"
     CDS             2116..2658
                     /locus_tag="PLS34_09950"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRP87826.1"
                     /translation="MTMLMLGLVVFFGAHLVRVAAPAYRERMIARLGLLPWKAVYSVV
                     SLAGFLLILIGYGEVRWASPVLWGPAPGWVRMAVGLAMLPVLVVFLSAYLPGRLRAAL
                     RHPMMLATVAWAALHLLVNGRVADLVLFGGFLAWALVVLFDSYRRPWRCGTALRSRPA
                     APRGGGWPSAAATRSCSAYR"
     gene            complement(2731..>4649)
                     /locus_tag="PLS34_09955"
     CDS             complement(2731..>4649)
                     /locus_tag="PLS34_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRP87827.1"
                     /translation="PKEYGGLDFSARAQSEVVVKIASRSGTAAVTVMVPNSLGPGELL
                     MHYGTHEQKQHYLPRLARGEEIPAFALTGPWAGSDAGSMPDIGVVCKGEYKGKEVLGF
                     RVNWQKRYITLGPIATLLGLAFKAEDPDGLLGGKRELGITCALIPTGTPGVEIGLRHN
                     PGGAFMNGPNSGKDVFVPMDWVIGGQAQVGNGWKMLMDCLSVGRAISLPALGTAGGKA
                     AARMSGAYARVRRQFKIPIGRFEGIEEPLARIAGVTYRMDAARVLTATALDLGGKPSV
                     LSAILKYNNTEGMRQAINDAMDIHAGRAVCLGPSNYLQQTYQTVPVAITVEGANILTR
                     SMIIFGQGAIRCHPYVLKEMLAAANDDAQAGLKAFDEAFFAHVGFTISNLVRSFVLAL
                     TGARFVAAPDLGPTAVYAQRLTRMSASFALLADVAMLMLGGDLKRREKISGRFADILS
                     HLYMASAVLKRFEDDGRPAADLPIVRWALDDSLAIIEERLDGILRNFPVAGVGAVLRP
                     FVLPLGRRHKGASDRLGHEVASLLLSPSETRDRLTDGLFMDLKRDDPMGLMELALEKV
                     IAAEAVERKLAKGLGASVNTLNMEAVLAKGVAEGVINDLEAEEVREAMAITAEAIAVD
                     DFPGKPKKAGQQAA"
BASE COUNT          710 a         1554 c         1613 g          772 t
ORIGIN      
        1 cgcgacgagc tgactcgcca cgctgcgaac gacggcgtgt gggacttcga cgtggagacc
       61 aacaccatcc gcttctcgcc ccgctggaag gtcatgatgg gctacggcga ggacgagccc
      121 gacgaggaca tgcccgactg gcgccagctc gtgcatcccg aggacatggt gcgggtgcag
      181 agcaagattc gcgatcacct cgaaggcaag agcgagctgt tcgagagcgt gcaccgcatg
      241 cggcaccgct cgggcgactg gcgctgggtc atcagccgcg ccaaggccat ggtggacgac
      301 gatggccgca tgctgcgcct ggttggcgtg gagctcgaca tcacggacca gaagctctac
      361 gaggaggcgc tgtttcgcga gaagcagaat gcccagatca cgctgcagtc gatcggcgac
      421 ggcgtcatca cgacggattc cgactgccgc atcgagtacc tcaacccagt ggcgcaggag
      481 ctgaccggct gggccacgga ggacgccgcc ggccgctcgg tggacgagat tttccgcagt
      541 ttccacgaag agacctgcga gccgatggag aacccgctgt cggtggccat ccgccgcgat
      601 cgcctctaca agtcgatgcg gccgacgctg ctgatccggc gtgacggcaa cgaactgtac
      661 gtcgagagct gcgcggcgcc gatccgcaac gacatgggcc aggtgatcgg cggcgtgctg
      721 gtgttccacg acgtgagcga ggcgcgcgag ctcaatcgcc ggctgtccta ccacgccagc
      781 cacgacatgc tgaccggcct ggtgaaccgg cgcgagttcg aggcgcggct ggagcgggcg
      841 ctcaagatgg ggcgcgcgcg cgaggcgcag tacgcgctct gctacatgga cctcgaccag
      901 ttcaagatca tcaacgattc ctgcgggcac atggccggcg acgccttgct cggccagctc
      961 ggcgcgctgc tgaagtcgaa gatccgctgg cgcgacacgc tggcgcggct cggcggggac
     1021 gaattcggcg tgctgctcga gaactgcacg ctcgacgagg ccatcgagac ggccgaggcg
     1081 ctgctcaagg ccgtgcagga cttccgcttc gtgtgggagg atcgcagctt ccggctcggc
     1141 gccagcatcg gcgtggtgcc gatcgtgccg gagaccgcca gcgtggcgga gctgctgacg
     1201 gcggctgaca gcgcctgcgc ggcggccaag gaatccggcc gcaaccgcgt gcagacctgg
     1261 caggaaaacg acatcgacct gatgcgccgg cgccgcgaga tgcaatgggc ggcgcgcatc
     1321 aacaacgcgc tggaggaatc gcgcttcgag ctgttccgcc agccgatcat gcccttgcac
     1381 gaccagtcca gcggcgcgca ctacgagctg ctgctgcgca tgcgcgacga gcagggccag
     1441 atcatcgcgc cggatgtgtt catcaatgcc gccgagcgct acggcctgac gccgagcatc
     1501 gaccgctggg tgattaccaa cgccttccgc tggctggttt ccgaggccga cgagcgcgag
     1561 cggctcgcga tgtgctccat caatctctcc ggccagagcc tgtgcgacga caagttcctg
     1621 cccttcgtga tcgagcagtt cgagcgcagc gggctggacg cgtccaagat ctgcttcgag
     1681 atcaccgaga ccgccgccat cgccagctat tcgcaggcga atcgcttcat caattcgctg
     1741 aaggaactcg gctgccgctt cgcgctggac gatttcggca ccgggctgtc ttccttcggc
     1801 tacctcaagc acttcccggt ggatttcctc aagatcgacg gcagcttcgt gaaggagatc
     1861 ctgcacgacc cgatcgaccg cgagatggtc cgctcgatca acgagatcgg ccatctgacc
     1921 ggcaagcaga ccatcgccga gttcgtcgag aacaaggaac tcatcaccat gctgcgcggc
     1981 atgggcgtgg actacgcgca gggctacggc gtcggcgagc cgcggcgcat catggacccg
     2041 gagttcgcgg tccgcgagat tcgctagccc ggccggcctg ctaagctgtc cggagtttcc
     2101 tccggagagt tccccatgac gatgctgatg ctgggcctcg tggtgttttt cggcgcacac
     2161 ctggtgcgcg tggcggcgcc ggcgtaccgg gagcgcatga tcgccaggct ggggctgctg
     2221 ccgtggaagg cggtctattc ggtcgtgtcg ctggccggct tcctgctcat cctgatcggc
     2281 tatggcgagg tccgctgggc cagccccgtg ctgtggggtc cggcgcccgg ctgggtgcgc
     2341 atggccgtcg ggctggcgat gctgccggtg ctggtggtgt tcctgtcagc ctacctgccg
     2401 ggccggctgc gcgccgcgct gcggcacccg atgatgctcg ccacggtggc ctgggcggcc
     2461 ctgcacctgc tggtgaacgg gcgggtcgcc gacctggtgc tgttcggcgg cttcctggcc
     2521 tgggcgctgg tggtgctgtt cgattcgtac cggcggccgt ggcgctgtgg gacggcgttg
     2581 cgctcgcggc cggcggcgcc gcgtggtggt ggctggcctt cggcggcggc cacgcgctct
     2641 tgttccgcat accggtgatg taattgggga cattccccaa ttccgcgtgg aattggggag
     2701 tgtcccctat taatggggag tgtccccttt tcaggcggcc tgctggccgg ccttcttcgg
     2761 cttgcccggg aagtcatcca ccgcgatggc ctcggccgtg atggccatcg cctcgcgaac
     2821 ttcctcggcc tcgaggtcgt tgatcacgcc ctcggcgacg cccttcgcca gcaccgcttc
     2881 catgttcagc gtgttgacgc tcgcgccgag gcccttggcc agcttgcgct cgaccgcctc
     2941 cgccgcgatg accttctcca gcgccagctc catcaggccc atgggatcgt cgcgtttcag
     3001 gtccatgaac aggccgtcgg tgagccggtc gcgcgtctcg gagggactga gcagcagcga
     3061 cgccacctcg tggccgagac ggtcggaggc gcccttgtga cgacgcccca gcggcagcac
     3121 gaagggccgt agcactgcac ccacgcccgc caccgggaag ttgcgcagga tgccgtcgag
     3181 ccgctcctcg atgatcgcca gcgaatcgtc cagcgcccag cgcacgatcg gcaggtcggc
     3241 ggccggccgg ccgtcgtcct cgaagcgctt gagcaccgcc gaggccatgt agaggtggct
     3301 gaggatgtcg gcgaagcgcc cggagatctt ctcgcgccgc ttcaggtcgc cgcccagcat
     3361 cagcatcgcc acgtcggcga gcagcgcgaa ggaggcgctc atgcgcgtca ggcgctgcgc
     3421 gtaaacggcg gtcgggccga gatccggcgc cgcgacgaag cgcgcgccgg tgagcgccag
     3481 cacgaaggag cgcaccaggt tgctgatggt gaagccgaca tgcgcgaaga aggcctcgtc
     3541 gaaagccttg agccccgcct gggcgtcgtc gttggccgcg gcgagcattt ccttcagcac
     3601 gtagggatgg cagcggatcg ccccctggcc gaagatgatc atgctgcgcg tgaggatgtt
     3661 ggcgccctcg accgtgatgg ccaccggcac cgtctggtag gtctgctgca ggtagttgct
     3721 cggcccgagg cacaccgcgc gcccggcgtg gatgtccatc gcgtcgttga tggcctggcg
     3781 catgccctcg gtgttgttgt acttgaggat cgccgacagc accgacggct tgccgccgag
     3841 atccagcgcg gtcgcggtca gcacgcgcgc cgcgtccatg cggtaggtga cgccggcgat
     3901 gcgcgccagc ggctcctcga tgccctcgaa gcggccgatc gggatcttga actggcggcg
     3961 cacccgcgcg taggcgccgg acatgcgcgc cgccgccttg ccgccggccg tgccgagcgc
     4021 cggcagggag atggcgcggc cgactgacag gcagtccatc agcatcttcc agccgttgcc
     4081 cacctgggcc tgtccgccga tcacccagtc catcggcacg aacacgtcct tgccgctgtt
     4141 cgggccgttc atgaaggcgc cgccgggatt gtggcgcagg ccgatctcga cacccggcgt
     4201 gccggtcgga atcagcgcgc acgtgatgcc cagctcgcgc ttgccgccga gcaggccgtc
     4261 ggggtcctcg gccttgaagg ccagcccgag gagggtggcg atcggtccga gcgtgatgta
     4321 gcgcttctgc cagttgacgc ggaagccgag cacttccttg cccttgtact cgcccttgca
     4381 gaccacgccg atgtccggca tggagccggc gtcggacccc gcccaggggc cggtcagggc
     4441 gaaggcgggg atttcctcgc cgcgtgcgag ccgcggcagg tagtgctgct tctgctcgtg
     4501 ggtgccgtag tgcatcagca gctcgccggg ccccagcgag ttcggcacca tgaccgtcac
     4561 cgcggcggtg ccgctgcggc tggcgatttt caccaccacc tccgactggg cacgcgccga
     4621 gaagtccagc ccgccgtact ccttgggaa
//
LOCUS       DAOMPG010000192         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7756_length_4643_cov_3.161508, whole genome shotgun sequence.
ACCESSION   DAOMPG010000192 DAOMPG010000000
VERSION     DAOMPG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7756_length_4643_cov_3.161508"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLS34_09960"
     CDS             <1..205
                     /locus_tag="PLS34_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87828.1"
                     /translation="GAGLSPRAAIALLRSARAWALLDGRRAVIPEDVQAVTAAVVGHR
                     LRADGDVAGADAGERLVAAVPIP"
     gene            180..1169
                     /locus_tag="PLS34_09965"
     CDS             180..1169
                     /locus_tag="PLS34_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRP87829.1"
                     /translation="MSLRSPFPDLGALARGWARRRQGPDGEAVELHRRRIYILPTALG
                     LGYGMSLFGMLLAAMNYNNNMGFALTFLLAGLGLVTMHWAHQNLSGVIVRGIRLQPAF
                     AGQPVRLDLSLQNTGRAPRHDLQAGCAAARSAFLDLAPGQAGELHLHLSAQHRGRLAL
                     DRIGVHTTFPFGLFRAWAWLHPECATVIYPAPAEHAPTPPAGGPEDPDGRWDEHRGED
                     DFAGLRDFRQGDSPRRIAWKAAARGDELPVKQFAGSARGSAWLDWDSLPRLPLEERLS
                     VLCRWVLDAHAGGGAFGLRIPGTEIPPGMGPAHRHRCLAALALYQRDDESAHV"
     gene            1162..3117
                     /locus_tag="PLS34_09970"
     CDS             1162..3117
                     /locus_tag="PLS34_09970"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRP87830.1"
                     /translation="MSDPAKLRLGWMLAALAVALAPAVPRLPPWIAATFAIAALWRLA
                     VQQFGWRLPPGWLRLLLSLGCVLGVLFTYRTFNGLEAGGGLLVVMGAMKLLETRSRRD
                     IQVLSFIGFFLVLMQLLYDQPIWSLPWLVLSVIAIVLALLQGVRGGEPLPWRVATGVV
                     LRMLAFAAPVALVLFLLFPRVPGPFWTLPSRGPAGLTGLSDEMSPGRIAQLIQSDEVA
                     FRVSFEGPLPPPSQRYWRGPVLERFDGWTWSDLDLLPLLQQRMELRGRSYRYRITVEP
                     HGRAWLLALDYPARWDDREALLTNEFQLVARKPVDSTRALEVESWPDARPEPSLSPFM
                     RMRTLRLPADRNLRTAAFAQELRATAASDADFIDAVLAHFRNEPFVYTLRPPLLGGGH
                     PVDDFLFRTRRGFCEHYASAFTMLARAAGIPARVVTGYQGGELNPISNRLVVRQSEAH
                     AWSEVWLEGRGWTRVDPTAAVAPERVELSLAEALPAGERVPGGLLRSLAGLRELSQGW
                     DALNSLWTDWVLGYGPERQLALLARLGLPGADWRALVVGLTVLITAAMALLTLWLAWR
                     LRAPPEPAALRLYRQFCRRLETTGLLRAPQEGPQDFAARVAAERPALAQVVARVTRLY
                     TALRYEAAGDDAALLDELRREVRAFRR"
     gene            complement(3151..>4643)
                     /locus_tag="PLS34_09975"
     CDS             complement(3151..>4643)
                     /locus_tag="PLS34_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRP87831.1"
                     /translation="FGGAIQMAGSVKGRKSDRHATSDWMALEQQRGISVTSSVMQFPY
                     RDKIVNLLDTPGHADFSEDTYRTLTAVDSALMVIDAAKGVEERTIKLMEVCRLRTTPI
                     ISFINKLDRDGREPLALLDEIERVLAIRCTPVTWPIGMGREFRGVYHFLQDRIYIYRE
                     AARGAAGQTEVIDGLDSAAAGELLGSRADEFREEVELVRGACDSFDREAYLAGAQTPV
                     FFGSAISNFGVRELLDGFVDWAPPPASRATTTRTVEAGEPALTGFVFKIQANMDPAHR
                     DRIAFFRVCSGRYSRGMKLRHVRLGRDVRIADALTFMAADRERAEEAWAGDILGLHNH
                     GTINIGDTFTEGEELTFTGIPNFAPELFRRAVLGDPLRAKALHKGLDQLCEEGATQLF
                     RPLRNNDVILGAVGILQFDVVAWRLKEEYGVECRFEPINVATARWVYCDDEKRFAEFR
                     NKAAEHLAVDHADALVYVAPSRVNLELTRERWPEVRFMPTRDHLAA"
BASE COUNT          578 a         1673 c         1685 g          707 t
ORIGIN      
        1 cggcgccggc ctgtcgccgc gcgccgccat cgccctgctg cgcagcgcgc gcgcttgggc
       61 gctgctggac ggccgccgcg cggtcatccc cgaggacgtg caggccgtca ccgcggcggt
      121 ggtcgggcac cggctgcgcg ccgacggcga cgtggcgggc gccgacgcgg gagaacggct
      181 tgtcgctgcg gtccccattc cctgatctcg gcgccctggc gcgcggctgg gcacgccgcc
      241 gccagggacc ggatggcgag gccgtggagc tgcaccgccg gcgcatctac atcctgccga
      301 cggcgctcgg tctcggttac ggcatgagcc tgttcggcat gctgctcgcg gccatgaact
      361 acaacaacaa catgggtttc gcgctgacct tcctgctggc gggcctcggc ctggtgacca
      421 tgcactgggc gcaccagaac ctgagcggcg tgatcgtgcg cggcatccgg ctgcagccgg
      481 ccttcgccgg ccagccggtg cggctggacc tgtcgctgca gaacaccggg cgcgcaccgc
      541 gccacgacct gcaggccggc tgcgccgcgg cgcggtcggc cttcctcgac ctcgccccgg
      601 gccaggcggg cgagttgcac ctgcacctgt cggcgcagca ccgcggccgg ctcgcgctgg
      661 accggatcgg cgtgcacacg acgtttccat tcgggctgtt ccgcgcctgg gcgtggctgc
      721 accccgaatg cgccacggtg atctacccgg cgccggcgga gcatgccccg acgccgcctg
      781 cgggcggccc cgaggatccg gacggacgct gggacgagca ccgcggcgag gacgatttcg
      841 ccgggctcag ggacttccgc cagggcgact cgccgcgacg catcgcctgg aaggccgccg
      901 cgcgcggcga cgagctgccg gtcaagcaat tcgccggcag cgcccgcggc agcgcctggc
      961 tggactggga cagcctgccc cggctgccgc tcgaggagcg cctgtcggtg ctgtgccgct
     1021 gggtgctcga cgcccacgcc ggcggcggcg ccttcggcct gcgcatcccc ggcacggaga
     1081 ttcccccggg gatgggcccg gcgcaccggc atcgctgcct ggcggcgctg gcactgtacc
     1141 agcgcgacga tgagtccgcg catgtctgac ccggccaagc tgcgcctcgg ctggatgctg
     1201 gcggcgctcg cggtggcgct ggcgcccgcc gtaccgcgcc tgccgccgtg gatcgcggcg
     1261 accttcgcga tcgccgcgct gtggcgcctg gccgtgcagc agttcggctg gcgcctgccg
     1321 cccggctggc tgcgcctgct gctgtcgctc ggctgcgtgc tcggcgtgct gttcacctac
     1381 cggaccttca acggcctgga ggccggcggc ggcctgctgg tggtgatggg ggcgatgaag
     1441 ctgctcgaga cgcgcagccg gcgcgacatc caggtgctgt ccttcatcgg cttcttcctg
     1501 gtgctgatgc agctgctcta cgaccagccg atctggagcc tgccgtggct ggtgctgagc
     1561 gtgatcgcga tcgtgctcgc gctgctgcag ggcgtgcgcg gcggcgagcc gctgccatgg
     1621 cgcgtggcga ccggcgtcgt gctgcgcatg ctggccttcg ccgcgccggt cgcgctggtg
     1681 ctgttcctgc tgttcccgcg cgtgccgggc ccgttctgga ccctgccatc acgcggcccc
     1741 gcggggctca ccggcctgtc ggacgagatg tcgccgggcc gcatcgcgca gctcatccag
     1801 tcggacgaag tggcctttcg cgtcagtttc gaaggcccgc tgccgccgcc ctcgcagcgc
     1861 tactggcgcg gcccggtgct ggagcgcttc gacggctgga cctggagcga cctggacctg
     1921 ctgccgctgc tgcagcaacg catggaattg cgcggccgct cgtatcgcta tcgcatcacc
     1981 gtcgagccgc acggccgggc ctggctgctg gcgctggact atccggcccg gtgggacgac
     2041 cgcgaggcac tgctcaccaa tgaattccag ctggtggcgc gcaagcccgt cgacagcacg
     2101 cgcgccctgg aagtggagtc gtggcccgac gcgcgccccg agccgagcct cagccccttc
     2161 atgcgcatgc gcacgctgcg cctgccggcg gaccgcaacc tgcgcactgc cgccttcgca
     2221 caggagctcc gcgcgacggc cgcctccgac gcggatttca tcgatgcagt gctggcgcat
     2281 tttcgcaacg aaccgttcgt ctacaccttg cgcccgccgc tgctcggcgg cggccacccg
     2341 gtggacgatt tcctgttccg cacgcggcgc ggcttctgcg agcactacgc ctccgccttc
     2401 accatgctgg cgcgggccgc ggggattccg gcgcgcgtgg tcaccggcta ccagggcggc
     2461 gagctgaatc ccatcagcaa ccggctggtg gtgcgccagt ccgaggcgca tgcctggtcg
     2521 gaggtctggc tggagggccg cggctggacg cgggtggatc ccaccgctgc ggtggcgccg
     2581 gagcgcgtgg aactgagcct ggccgaggcc ttgccggcgg gcgagcgcgt gcccggcggg
     2641 ctgctgcgca gcctggccgg cctgcgcgag ctgagccagg gctgggacgc gctcaacagc
     2701 ctgtggaccg actgggtgct cggctacggt ccggagcgcc agctcgcact gctggcgcgg
     2761 ctcggcttgc ccggcgccga ctggcgtgcg ctggtcgtcg ggctgaccgt gctaatcacc
     2821 gcggccatgg cgttgctgac gctgtggctg gcgtggcggc tgcgcgcgcc gcccgagccg
     2881 gccgcgctgc ggctctatcg ccagttctgc cggcgcctcg agacgacggg actactgcgc
     2941 gcgccgcagg agggaccgca ggacttcgcc gcgcgcgtgg cggccgagcg cccggcgctg
     3001 gcacaggtcg tggcgcgcgt cacgcgcctg tacacggccc tgcgctacga agcggccggc
     3061 gacgacgccg ccctgctgga cgagctgcgc cgcgaagtgc gcgccttccg ccgctagggc
     3121 ctgtttacac tcgccggatt gcagcgccgc ttacgcggcg aggtggtcgc gcgtcggcat
     3181 gaagcggacc tccggccagc gctcgcgcgt cagctccagg ttgacccgcg agggcgcgac
     3241 gtagaccagt gcgtcggcat gatcgacggc gagatgctcg gccgccttgt tgcggaactc
     3301 cgcgaagcgt ttctcgtcgt cgcagtacac ccagcgggcg gtggcgacgt tgatcggctc
     3361 gaagcgacat tcgaccccgt attcctcctt gagccgccag gcgacgacgt cgaactggag
     3421 gatgccgacg gcgccgagga tcacgtcgtt gttgcgcagc ggccggaaca gctgcgtggc
     3481 gccctcttcg cagagctggt ccagaccctt gtgcagcgcc ttggcgcgca gcggatcgcc
     3541 gagcacggcg cggcggaaca gctccggcgc gaagttcggg atgccggtga aggtgagttc
     3601 ctcgccctcg gtgaaggtgt cgccgatgtt gatggtgccg tggttgtgca ggccgaggat
     3661 gtcgcccgcc caggcttcct cggcgcgctc gcggtcggcc gccatgaagg tcaacgcgtc
     3721 cgcgatgcgc acgtcgcgcc cgagccgcac gtggcgcagc ttcatgccgc gggaatagcg
     3781 cccggagcag acgcgaaaga aggcgatgcg gtcgcggtgc gccgggtcca tgttcgcctg
     3841 gatcttgaac acgaagccgg tcagcgccgg ctcgcccgcc tcgaccgtgc gcgtggtggt
     3901 ggcgcgcgag gccggcggcg gcgcccagtc gacaaagccg tcgagcagct cgcgcacgcc
     3961 gaaattgctg atggccgagc cgaagaacac cggcgtctgc gcgcccgcga gataggcctc
     4021 gcgatcgaag gagtcgcagg cgccgcgcac cagctcgacc tcctcgcgga actcgtcggc
     4081 ccgggagccc agcagctcac cggccgccgc agaatccagg ccgtcgatca cctccgtctg
     4141 cccggcggcg ccgcgcgcgg cctcgcggta gatgtagatg cggtcctgca ggaagtggta
     4201 cacgccgcgg aactcccgcc ccatgccgat cggccaggtg accggcgtgc agcggatggc
     4261 cagcacgcgc tcgatttcgt cgagcagcgc cagcggctcg cggccgtcgc ggtcgagctt
     4321 gttgatgaag ctgatgatcg gcgtggtgcg cagccggcac acctccatca gcttgatggt
     4381 gcgctcctcc acgcccttgg cggcgtcgat caccatcagc gcggagtcca ccgccgtcag
     4441 cgtgcggtag gtgtcctcgg aaaagtccgc gtggcccggc gtgtccagca ggttgacgat
     4501 cttgtcccgg taagggaact gcatcaccga ggaggtcacc gagatgccgc gctgctgctc
     4561 cagcgccatc cagtccgagg tcgcatgccg atcggacttg cgccccttga cggagcccgc
     4621 catctggatg gcaccgccga aca
//
LOCUS       DAOMPG010000193         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7816_length_4618_cov_3.633355, whole genome shotgun sequence.
ACCESSION   DAOMPG010000193 DAOMPG010000000
VERSION     DAOMPG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7816_length_4618_cov_3.633355"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLS34_09980"
     CDS             complement(<1..488)
                     /locus_tag="PLS34_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRP87832.1"
                     /translation="MAGIGTVPIGAYLGPDVYVGRYQWSPDSRRIAMEIVDQRAVRRV
                     PFPSYLHDEPLLHEARRPYPGDTDLLRRMAVYDLASGALEYLDLEAPNRRLVLDFEWS
                     PSGDALLVMQGADVAEERWIYIVSGADLRLRELWHDQRPRRIYPAFRALCSADGKQIV
                     FVG"
     gene            complement(1018..1392)
                     /locus_tag="PLS34_09985"
     CDS             complement(1018..1392)
                     /locus_tag="PLS34_09985"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRP87833.1"
                     /translation="MKPPSQQPAIRMMMMPKDTNAVGSIFGGVILSLIDQAAFVEASR
                     QAARKFVTIAMDKVEFHKPVHNGDIVSLWAETIHIGRSSIRLRVDVHARSRNRDEDIR
                     VTSAEVTMVSIDEAGRPTPIFP"
     gene            complement(1411..2277)
                     /locus_tag="PLS34_09990"
     CDS             complement(1411..2277)
                     /locus_tag="PLS34_09990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP87834.1"
                     /translation="MQRSLEPQVVVDTFVRTLREGFDVDGVAFDHPEYGRQWGARGDH
                     AFATRLKLDGAALGKLEFYRRQAFGRRERSELNELAELLLRPLHNALTHACLQKQAFE
                     DGLTGLLNRQALERMLPRELAAAERLGRPLPLVMIDVDLLKPINDTWGHAAGDRALKN
                     MAAAISGALRQSDLAFRLGGDEFMLLLPATDEAGARTVVERVREMLSRDAGLRQAGTG
                     PDGRNVTFSAGIAVGTPGVSAADLIEQADRAMYRAKHAGGNRDGTAARVRARPRAPDF
                     AAERIAAKMTRR"
     gene            complement(2566..3414)
                     /locus_tag="PLS34_09995"
     CDS             complement(2566..3414)
                     /locus_tag="PLS34_09995"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRP87835.1"
                     /translation="MTEHQQAFAIVQKLAAELSSGQVELPSLPRAVVRLRNELAKPGF
                     DVPRLAKLAATEPALAGSILTMANSVAFRRSGSETLDLKVAIPRIGADKVQALALRFA
                     TRQLSNGQDLGRGRKFLEREWAQGLRVAATCYLVAEHSRDVNPDEALIIGLIHNVGRI
                     YLLSRADEHPALFDSPAILMELVESWHASVAQAIVESWSLPAEAAEAVAVQSEEDGLP
                     HGMLTYVLRIALALAGTSGAAPDAEALQAMAQWRSCRALKLSAADLATIREGQEAARL
                     NLGLTD"
     gene            complement(3427..>4618)
                     /locus_tag="PLS34_10000"
     CDS             complement(3427..>4618)
                     /locus_tag="PLS34_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRP87836.1"
                     /translation="HVNAAFREPLVPADPAGCGRWTPGTTAVHLPGKTTPDLAAVAAA
                     AARLDGRRGAILAGRLPPGDPAAAAVIGLALALDCPVIADPLSGLRAGFDAPARVLCA
                     ADAFLREPAAPLPEWLIRVGMPPVSRVVEEWAARCQDTLLLAATPAWSDPLRAARSVL
                     LGDPAATLAVLTAAVSRAGLRAAAWDALDQLEAAAAARLARLDAPPVEAALVHAIAAS
                     APAGTPVFVSNSLVVRDFDSFLPRRAAPLPLYANRGVSGIDGNLSTAAGLVTGAGRPG
                     IAVLGDLALLHDLNALSLCRDLPLVVIVINNGGGAIFGQLPQATLPEFDRLWLTPPAL
                     DIAGAAALFGLSHARLDGPTDFGQAIVSALESPRAMLLELVVDRAASHAGRKAWWTTP
                     QKRR"
BASE COUNT          674 a         1620 c         1691 g          633 t
ORIGIN      
        1 ccgacgaaga cgatctgttt gccgtcagcg ctacacagcg cgcgaaacgc cggatagatg
       61 cgccgtggcc gctggtcgtg ccacagctcc cgcagcctga gatcggcgcc ggatacgatg
      121 tagatccagc gctcctcggc gacgtcggcg ccctgcatca ccagcagggc gtcgccggac
      181 ggcgaccatt cgaaatccag caccaggcgg cggttcggcg cctcgaggtc caggtactcg
      241 agcgctccgg acgccaggtc gtagacggcc atgcgccgca gcaggtccgt gtcgcccgga
      301 taagggcgtc gcgcctcgtg cagcagcggc tcgtcgtgca ggtaggaggg gaacggcacg
      361 cgccgcactg cgcgctgatc gacgatttcc atcgcgatgc ggcgcgagtc cggactccac
      421 tggtagcgcc cgacatagac atccggcccg aggtaggcgc cgatcggtac cgtgccgatg
      481 ccggccacgg cgatctcggt ggcgcgttgc ggcgcgccgt cgtcgagcgg cagcagccac
      541 aggtcgccgc cgcgcaggaa cgccggcccg cgccgccggt ggcgatggca gcgtccttgc
      601 cgtccagcga aatgcggcgc aactcgccgt cgagcacgta gaacagcgag cggccgtccg
      661 gatgccaggc gaggtcggcg atgccgccgt gttcgcgccg cgccgcgcgc gcgctgaggg
      721 ccgccagcga caggtcaggc ccggggccca gcaccgcggc gggctccggc gcgtggcggg
      781 tcagccgctg cgggacggca tcggccgccg ccgcgaccca caagtcgcga aacggcatgc
      841 catggtcgtt ccacaggaac gccacccggc gactgtcggg cgaccattcc gggcgggtcg
      901 gcgcggtgcc gtcgagcttc ggcagcgaga cgatgcgctc gacgccgggc gctgccgccg
      961 gctcggccgg cgtcgccgcc gggacgaccg ccagcaggag cagggcgaag caggccctca
     1021 cgggaagatc ggggtgggcc ggccggcctc gtcgatggag accatggtga cctcggcgct
     1081 ggtcacgcgg atgtcttcgt cgcggttgcg cgagcgggca tgcacgtcga cgcgcagccg
     1141 gatggagctg cggccgatgt gaatggtctc cgcccagagg ctgacgatgt cgccgttgtg
     1201 cacgggcttg tggaattcca ccttgtccat ggcgatggtg acgaacttgc gcgcggcctg
     1261 gcggctggcc tcgacgaagg cggcctggtc gatcagcgac aggatgacgc cgccgaagat
     1321 cgagcccacg gcgttggtgt ccttcggcat catcatcatg cggatggccg gttgctggct
     1381 ggggggtttc aagagcgact ctccgtggcg tcaacgacgc gtcattttcg ccgcaatgcg
     1441 ctccgccgca aagtcgggcg cgcggggccg cgcccgcacg cgcgctgccg tgccgtcgcg
     1501 attgccgccg gcgtgcttgg cgcggtacat ggcgcgatcc gcctgctcga tgaggtcggc
     1561 cgccgacacc ccgggcgtgc cgacggcgat gccggcgctg aaggtgacgt tccggccgtc
     1621 cgggccggtg cccgcctggc ggagtccggc gtcgcgggac agcatctcgc gcactcgctc
     1681 gacgacggtc ctggcgccgg cctcgtcggt ggccggcagc agcagcatga actcgtcgcc
     1741 gcccagcctg aacgccaggt cggattggcg cagtgcgccg gagatggccg cggccatgtt
     1801 cttgagggcc cggtcgcccg cggcgtggcc ccatgtgtcg ttgatgggct tgagcaggtc
     1861 gacgtcgatc atcaccagcg ggagcggccg gccgaggcgt tccgccgcgg ccagctcgcg
     1921 cggcagcatc cgctcgaggg cctggcggtt gagcaggccg gtcagcccgt cctcgaaggc
     1981 ctgcttctgc aggcaggcgt gcgtcagcgc gttgtgcagc gggcgcaaca gcagctcggc
     2041 cagttcgttc agctcgctac gctcgcgccg gccgaacgcc tggcggcggt agaactccag
     2101 cttgccgagc gcggcaccgt ccagcttgag gcgggtggca aaggcgtggt cgcccctggc
     2161 gccccactgg cgcccgtact cggggtgatc aaaggccacg ccgtccacgt cgaagccctc
     2221 acgcagcgtg cgcacgaagg tatccaccac gacctgcggc tccagcgagc gctgcaatgc
     2281 gcgcagcagc gccagcggct gggcgcgcgc gcggcggctt gggcgaggtc ggtgtggcga
     2341 cagtggcgct cgggcgcgat gccatggcgt caaattcccg gaaaacagtg gcttaggacc
     2401 tgcgaatatc atgccaatca cgacaatgct cgtaactcgt tgattgttat gaattgagtg
     2461 cgcccgccaa gcgtgggccg gtcgggtatg gccgctgggt cggccaggaa agtgtgaacg
     2521 ggttccgccg gcgacgggaa atcgtcgcgc ggtggcaggg ccgcgctagt cggtgagccc
     2581 caggttcagg cgggccgctt cctggccttc acggatggtg gcgaggtccg ccgcggacag
     2641 cttcagcgcg cggcaggagc gccactgcgc catggcctgc aacgcctcgg cgtcgggcgc
     2701 tgcgcccgag gttccagcca gggccagcgc gatgcgcagc acgtaggtca gcatcccgtg
     2761 cggcaggccg tcctcttccg actgcacggc caccgcctcg gcggcctccg cggggaggct
     2821 ccaggactcg acgatcgcct gcgcaacact cgcgtgccag ctttcgacca gttccatgag
     2881 gatggcaggc gagtcgaaca gcgccgggtg ctcgtcggca cggctcagca ggtagatgcg
     2941 gccgacgttg tggatcaacc cgatgatcag cgcctcgtcg gggttgacgt cccggctgtg
     3001 ctccgccacc aggtaacagg tcgccgcaac ccgcaggccc tgcgcccact cccgctcgag
     3061 gaacttccgg ccccggccga gatcctggcc gttgctcagc tggcgcgttg cgaagcgcag
     3121 cgccagcgcc tgcaccttgt cggcgccgat gcgcggaatg gccaccttga ggtcgagggt
     3181 ttcactgccg ctgcgcctga acgccaccga gttcgccatg gtgaggatcg agcctgccag
     3241 tgccggctcg gtcgccgcca gcttggcgag ccgcggtacg tcgaagccgg gcttggcgag
     3301 ttcgttgcgc aggcgcacca cggcgcgggg cagggaaggc agctcgacct ggccagagga
     3361 cagttcggcg gccagcttct gtacgatcgc gaatgcctgc tggtgctccg tcattcgtgc
     3421 tccccgtcag cggcgcttct gcggcgtcgt ccaccaggcc ttgcggccgg cgtggctggc
     3481 cgctcgatcg acgaccagct cgaggagcat ggcgcgcggc gactccagcg cactcactat
     3541 agcctgtccg aagtcggtcg ggccgtcgag gcgtgcatgc gacaggccga acagtgccgc
     3601 cgcaccggcg atgtccagcg cgggcggcgt cagccagagc cggtcgaact ccggcagcgt
     3661 cgcctgcggc aactggccga aaatcgcgcc gccgccgttg ttgatcacga tgacgacgag
     3721 cggcaggtca cggcacagcg acagggcgtt caggtcgtgc agcagcgcga ggtcaccgag
     3781 caccgcgatg cccggacggc ccgcgcccgt caccaggccg gccgccgtcg agaggttgcc
     3841 gtcgatgccg ctcacgccgc ggttggcgta caacggcagc ggtgcggcgc gccgtggcag
     3901 gaagctgtcg aaatcccgca ccaccagcga attggacacg aacaccggcg tcccggcggg
     3961 cgcggacgcc gcgatggcgt gcaccagtgc ggcctcgacg ggtggcgcat cgagcctcgc
     4021 cagccgcgct gccgccgcgg cctcgagctg gtccagcgca tcccacgcgg cggcgcgcag
     4081 cccggcgcgc gagacggcag ccgtcagcac cgccagggtg gcggccggat cgccgagcaa
     4141 caccgaccgg gcggcgcgca gcgggtcgct ccatgccggc gtcgctgcga gcagcagcgt
     4201 gtcctggcag cgcgccgccc actcctcgac gacgcgcgaa accggcggca ttcccacgcg
     4261 aatcagccac tccggcaacg gcgccgccgg ctcgcgcagg aacgcgtcgg ccgcgcacaa
     4321 tacgcgcgcc ggcgcatcga atcccgcccg cagtccggac agcgggtcgg cgatcacggg
     4381 acaatccagc gccaacgcca ggccgatgac ggccgccgcg gccggatcac ccggcggcag
     4441 ccggccggcg aggatggcgc cgcgccgccc gtcgaggcgt gccgccgctg cagccaccgc
     4501 ggcgagatcg ggggtggtct tgcccggcag atggactgcc gtcgtaccgg gggtccagcg
     4561 gccacagccg gccggatcgg cgggcaccag cggctcgcgg aacgcggcat tgacgtgc
//
LOCUS       DAOMPG010000194         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7842_length_4607_cov_4.222540, whole genome shotgun sequence.
ACCESSION   DAOMPG010000194 DAOMPG010000000
VERSION     DAOMPG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7842_length_4607_cov_4.222540"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLS34_10005"
     CDS             <1..1134
                     /locus_tag="PLS34_10005"
                     /inference="COORDINATES: protein motif:HMM:NF020876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HRP87837.1"
                     /translation="LKYHCTEMARVVANDAMDIHGGKAICMGPGNYLGTGYNSIPVMI
                     TVEGANILTRSLMIFGQGAIRCHPFVLKELHAAQDPDQEAGLEAFDRALFSHIGYALS
                     NAARALVLGLTAAKWADTPTRGPTRRYYQHIGRFSAAFALVSDTAMLTLGGALKKKEM
                     LSARLGDILSNLYLASMVLKHFENQGRREADLPLVEWSCRFLLYRAQEQLHGFLRNFP
                     VRPVAWLLRLVVFPRGRSFSSPRDRLARRIVEGVIGPGEMRDRLCDGVYRTVEPGNAL
                     GLLQEAMELSIEVAPLEKKLRKGVKEGQVHALDFAGQLEDGVAAGIITVEEAAQLRAA
                     DEKVMALIHVDDFAPEELQPLVQPGKPARKGRRAARSPESAEA"
     gene            1159..2496
                     /locus_tag="PLS34_10010"
     CDS             1159..2496
                     /locus_tag="PLS34_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRP87838.1"
                     /translation="MPGEGITYEVALQPDPEILADFEAWLEYHVDDMLALPGFTGASI
                     HKAEDPESGAQLRVVRYQLEDRAALDRYFEEFAPRMRAAGLAKFGDRFRASRRIVLEG
                     RDAALTDARPCPNCNELLWGQYCASCGQRARVRMITFWELLKDAGDLLASLDSRIWRT
                     LGLLMFRPGRLTLDYLQGKRARFVPPMRLFISATLVFFFIATLNAKFEFGPDVEFGPP
                     APPAPGAEAPASDAPANEAGDGSGAAGDTTGEEAGEAAASRPAESESRVQAGGMHFDG
                     DCNVSYEGVPDWLARLVPEERAREICERITVDHGRSFGQALLANVPAMMFLFLPLMAV
                     VMKVAYPLSGRYYAEHLLFLVHFHSFFFLLNIAVIVFRWGADLAAPGVLPLDLLTALA
                     YLYVPIYLFRAMRRVYAQGFWLTSFKYLLLGTAYFSALLVTFLGLLLYTAVTL"
     gene            2572..4329
                     /locus_tag="PLS34_10015"
     CDS             2572..4329
                     /locus_tag="PLS34_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP87839.1"
                     /translation="MYASFFGLNEKPFSITPDPRYLYLSRRHAEALAHLLYGVTDSGG
                     FIQLTGEVGTGKTTVVRSLLEQLPEHAEVALVLNPRLSPQEFLLTICDELHVQVPDRG
                     SQKSVVDALNSHLLQTHAAGRRVVLIVDEAQNLSAEVLEQIRLLTNLETAKQKLLQII
                     LIGQPELRDVLARSDLRQLAQRITGRYHLEPLEPDELRAYVRHRLEVAGSRAEIFSAG
                     ALKALHQASGGVPRLVNVIADRALLGAWSQETPTVSAGLTRKAAKEVFGERLRRRARR
                     SPGAAAILLGLAGVAIAVAAGIATSMIRERMAQPPVSSADQATGGRVERPAAADTSAA
                     VAAPPAGAVVLAPTTGPAEPAAETLPEGATAPLPDQETALVEPPPRLADQLAAGALAT
                     GTDTAFGALLARWGLAYRPDAGPACEQAAAQGLQCLLQRGTWGEIATLNRPAIIVLTD
                     RAGRDHQLLLNRVRDDSAELLAGESAVTVPLGDLLTLWFGEYLLLWRPEAGDGRVLSR
                     NATGADVLWLRRVLGELRGTPVLPAGSALYDPGLEAAVREFQRSRRLSVDGIAGAMTM
                     IAVNDALGLGGRPRLREDD"
     gene            4331..>4607
                     /locus_tag="PLS34_10020"
     CDS             4331..>4607
                     /locus_tag="PLS34_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87840.1"
                     /translation="MSLILDALRKSEHERQRQVGPGLAVVPESAERRRPATWLLVLGA
                     LLLLNFVVLAAVLLTGRPSEPAIVDRPLPAEAPPPAVQRPSATAGPMP"
BASE COUNT          646 a         1663 c         1602 g          696 t
ORIGIN      
        1 ctcaagtacc actgcacgga gatggcgcgc gtggtcgcca acgacgccat ggacatccac
       61 ggcggcaagg ccatctgcat gggtccgggc aactacctcg gcacgggcta caactcgatc
      121 ccggtgatga tcaccgtcga gggtgccaac atcctcacgc gcagcctgat gatcttcggc
      181 cagggcgcca tccgctgcca tcccttcgtg ctgaaggaac tgcacgccgc gcaggacccg
      241 gaccaggaag ccggcctgga agccttcgac cgcgccctgt tcagccacat cggctatgcg
      301 ctgtcgaacg ccgcgcgggc cttggtgctc ggactgacgg cggccaagtg ggcggacacg
      361 ccgacgcgcg ggccgacgcg ccgctactac cagcacatcg gccgcttcag cgccgccttc
      421 gcgctggtct ccgataccgc catgctgacg ctcggcggcg ccctgaagaa gaaggagatg
      481 ctgtccgcgc ggctcggcga catcctgtcg aacctctacc tcgcctccat ggtgctcaag
      541 catttcgaga accagggccg ccgcgaggcc gacctgccac tggtggaatg gagctgccgc
      601 ttcctgctgt accgggccca ggagcagctg cacggcttcc tgcgcaattt cccggtgcgt
      661 cccgtggcgt ggctgctgcg cctcgtggtg ttcccgcgcg gccgcagctt cagctcgccg
      721 cgcgaccggc tggcgcgccg tatcgtcgag ggcgtcatcg gccccggcga gatgcgcgac
      781 cggctgtgcg acggcgtcta ccgcacggtc gagccgggca acgcgctcgg cctgctgcag
      841 gaggccatgg agctgtccat cgaggtggcg ccgctggaga agaagctgcg caagggcgtc
      901 aaggagggcc aggtccacgc actcgatttc gccggccagc tcgaggacgg cgtggcggcc
      961 ggaatcatca ccgtcgagga agcggcacag ctacgcgccg ccgacgagaa ggtcatggcg
     1021 ctgatccacg tcgacgattt cgcgccggag gagctgcagc cgttggtgca gcccggcaag
     1081 ccggcgcgca agggacggcg cgcggccaga tcgcccgagt cggccgaggc ctaggcccgg
     1141 ccggcacgga ttgcgggcgt gcctggcgaa ggcatcacct acgaggtcgc gctgcagccg
     1201 gacccggaga tcctggccga cttcgaggcc tggctggagt accacgtgga cgacatgctg
     1261 gcgctgccgg ggtttaccgg cgccagcatc cacaaggccg aggacccgga atcgggggcg
     1321 cagttgcgcg tggtgcgcta ccagctcgag gaccgcgccg cgctggaccg ctacttcgag
     1381 gagttcgcgc cgcgcatgcg cgccgccggg ctggcgaaat tcggcgaccg cttccgcgcc
     1441 agccgccgca tcgtgctcga gggccgcgac gccgcgctga cggacgcgcg cccctgtccc
     1501 aactgcaacg agctgttgtg gggccagtac tgcgccagct gcggccagcg cgcgcgggtg
     1561 cgcatgatca ccttctggga gctgctcaag gacgccggcg acctgctcgc ctcgctggac
     1621 tcgcgcatct ggcgcaccct ggggctgctg atgttccggc cgggacgcct gacgctcgat
     1681 tacctgcagg gcaagcgggc gcgcttcgtg ccgccgatgc ggctgttcat ctcggcgacc
     1741 ctggtgttct tcttcatcgc cacactgaac gcgaagttcg agttcggccc cgacgtggag
     1801 ttcggtccgc cggcgccccc ggccccgggg gccgaagccc cggcgagtga tgccccggcg
     1861 aatgaagccg gcgacggcag cggcgccgcc ggcgatacga cgggcgaaga agcaggcgaa
     1921 gcggccgcat cgcgcccggc cgagtccgaa tcacgcgtgc aggccggcgg catgcacttc
     1981 gacggcgact gcaacgtcag ctacgagggc gtgcccgact ggctggcgcg gctggtgcca
     2041 gaggagcgcg ccagggagat ctgcgagcgg atcaccgtgg accacggccg gtccttcggc
     2101 caggccctgc tggcgaacgt gccggcgatg atgttcctgt tcctgccgtt gatggcggtg
     2161 gtcatgaagg tggcctaccc cctgtcgggg cgctactacg ccgagcacct gctgttcctg
     2221 gtgcatttcc actccttctt cttcctgctc aacatcgcgg tgatcgtgtt ccgttggggc
     2281 gccgacctgg cggcgcccgg cgtgctgccg ctcgacctgc tcaccgcgct ggcctacctc
     2341 tacgtgccga tctacctgtt ccgggcgatg cggcgggtct acgcgcaggg cttctggctg
     2401 acctccttca agtacctgct gctgggaacc gcttacttca gcgcgctgct ggtcacgttc
     2461 ctcggcctgt tgctgtacac cgcggtgacc ctgtagcgga ttgccccggc aaggctcccc
     2521 ggtgcatcat ccctgaccct gccgctacga aaggcggcgc ggatcaacgg catgtacgca
     2581 agcttcttcg gactcaacga aaaacccttc tccatcacgc cggaccccag gtacctgtac
     2641 ctgagccgcc ggcatgcgga ggcgcttgcg cacctgctgt acggcgtcac cgacagcggc
     2701 ggcttcatcc agctgaccgg cgaggtcggc accggcaaga ccacggtggt ccgcagcctg
     2761 ctggagcaac tgcccgagca tgccgaagtc gcgctggtgc tcaacccgcg cctctcgccg
     2821 caggagtttt tgctgaccat ctgcgacgag ctgcacgtgc aggtgcccga ccgcggcagc
     2881 cagaagtccg tcgtcgacgc gctcaacagc cacttgctgc agacgcatgc cgccggccgg
     2941 cgggtggtgc tgatcgtcga tgaggcccag aacctgtcgg ccgaagtact cgagcagatc
     3001 cgcctgctca ccaacctcga gaccgccaag cagaagctgc tgcagatcat cctgatcggc
     3061 cagccggagc tgcgcgacgt gctggcgcgc agcgacctgc gccagctggc ccagcgcatc
     3121 accggccgct atcacctcga gccgctggag cccgacgaac tgcgcgccta cgtccgtcac
     3181 cgtctcgagg tcgccggcag ccgcgccgaa atcttttccg ccggggcgct gaaggcattg
     3241 caccaggcct ccggcggcgt gccgcgcctg gtcaacgtga ttgccgaccg ggcgttgctc
     3301 ggtgcgtggt cgcaggagac gccgaccgtc tcggccggcc tgacgcgcaa ggcggccaag
     3361 gaggtgttcg gcgaacgtct caggcgccgt gcgcggcgct cgccgggcgc tgcggccatc
     3421 ctgctcggcc tcgccggtgt cgccatcgcc gtcgcagcgg gtatcgccac ctcgatgatt
     3481 cgcgagcgca tggcccagcc gcccgtgtcg tccgccgacc aggccaccgg cggcagggtc
     3541 gagcgaccgg ctgccgcgga cacgtcggct gcggtggctg ccccgcccgc gggggccgtc
     3601 gtcctggcgc ccacgaccgg cccggccgag cccgcggccg agactttgcc ggaaggcgcg
     3661 actgcgcctt tgcccgacca agagactgcg ctggtcgagc cgccgccgcg gctcgccgac
     3721 cagcttgcgg ccggagcgct ggcgaccggc accgacacgg ccttcggcgc gctgctggcg
     3781 cgctggggcc tggcctatcg gccggacgcc ggtcccgcct gcgagcaggc ggcggcccag
     3841 ggcctgcagt gtctcctgca gcgcggcacc tggggcgaga tcgccaccct gaaccggccg
     3901 gcgatcatcg tgctgaccga ccgcgccggg cgcgatcacc agctgctgct caaccgggtg
     3961 cgcgacgaca gcgcggagct gctcgccggc gagtctgcgg tcaccgtgcc gctcggcgat
     4021 ctcctgaccc tctggttcgg cgagtacctg ctgctctggc gaccggaggc cggcgacggg
     4081 cgcgtcctgt cgcgcaacgc gaccggtgcg gacgtgctct ggctgcggcg cgtgctcggc
     4141 gaactgcgcg gcacccccgt gctgccggcc gggtccgcgc tctacgaccc cggcctcgag
     4201 gcggcggtgc gcgagttcca gcggtcgcgc cgcctgagcg tggacggcat cgcgggcgcc
     4261 atgacgatga tcgcagtgaa cgatgcgctc ggcctcggcg gccgcccccg gctgcgggag
     4321 gacgattgag gtgtcgctga tcctcgatgc actgcgcaag tccgaacacg agcgtcagcg
     4381 ccaggtgggc cccggcctgg cggtggtgcc cgagagcgcg gaacgcaggc gtcccgcgac
     4441 ctggttgctg gtgctcggcg cactgctgct gctgaacttc gtggtgctgg cggcggtgct
     4501 gctcaccggg cgcccctccg agccggcgat cgtcgaccgg cccctgcccg ctgaagcgcc
     4561 gccaccagcc gtgcagcgcc cctcggcgac cgccgggccg atgcccg
//
LOCUS       DAOMPG010000195         4550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7947_length_4550_cov_3.498999, whole genome shotgun sequence.
ACCESSION   DAOMPG010000195 DAOMPG010000000
VERSION     DAOMPG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_4550_cov_3.498999"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLS34_10025"
     CDS             complement(<1..516)
                     /locus_tag="PLS34_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRP87841.1"
                     /translation="MPRDLPPAILAIGRNYAEHAAEMGSAAPDRPLVFMKNPASVVGD
                     GEAIVIPPICREHGPQVDFEGELAVIIGRDCRDVPASRALDYVRGYACANDVSARWWQ
                     KEGSGGQFCRGKSFDTFCPLGPVAPAGQIGDPQSLAIRTRLNGTVMQESNTRLMLFPV
                     ATLVAELSRGTT"
     gene            complement(601..690)
                     /locus_tag="PLS34_10030"
     tRNA            complement(601..690)
                     /locus_tag="PLS34_10030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Ser,seq:tga)
     gene            836..1195
                     /locus_tag="PLS34_10035"
     CDS             836..1195
                     /locus_tag="PLS34_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87842.1"
                     /translation="MQQIRIQLTGPGAGPGGHGGPGLVARILLSIGAVIMLVSAAFLG
                     AIVFLAALGFFVVAMLVLAVRIWWARRQLEKALRSGRPPGEGGPGRHQGDIEGEYRVL
                     DERRDAPAETRRRGGDR"
     gene            1155..1745
                     /locus_tag="PLS34_10040"
     CDS             1155..1745
                     /locus_tag="PLS34_10040"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRP87843.1"
                     /translation="MRPPRRAGAGEIAEVQDLKKDHRGLRRRPLYTPLTFVVGSVLAV
                     LVLAAWIVASWGSTTVVLVRHAEKVELGDDPGLSEAGLAHAQRLAEMLERAGIEAIYV
                     SQARRTQETAAPVAALAGVEPRVVRADQHGKLLRRLKWRHRSEVVLVVGHSNTVPVIA
                     DGLGAEIGLVDAEDYSGLWIISYSRLRGTRLLTLRY"
     gene            complement(1742..2284)
                     /gene="hemG"
                     /locus_tag="PLS34_10045"
     CDS             complement(1742..2284)
                     /gene="hemG"
                     /locus_tag="PLS34_10045"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HRP87844.1"
                     /translation="MKSVLVLYMSRGGHTARIARKICASIESAGGRGEMMDINEAVHE
                     GVDWDRYDAIALGAPVLYGTYDKSVFDFIAANQARLEARPSSFFNVSVVARTPEKATV
                     EGNRYMQKFLELSAWKPRDLKVIAGKVDYPSWGPLDTIAIQMIMKFTHGPTDRTAVID
                     YTNWDDVAAYGRHLLALAGR"
     gene            complement(2528..2980)
                     /locus_tag="PLS34_10050"
     CDS             complement(2528..2980)
                     /locus_tag="PLS34_10050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRP87845.1"
                     /translation="MMLKAGDHAPDFTLPDQDGRPVSLRNLLADGPLVLYFYPADFTP
                     GCTKEACSIRDMHDEIAAAGMRVVGISPQDATSHARFRDQHQLPFTLLADPGKAAVKA
                     WGVDGPLGVGVRRATFLVGADGVIRDAVLADLRISRHEEFIRKAIAAA"
     gene            complement(3011..3466)
                     /locus_tag="PLS34_10055"
     CDS             complement(3011..3466)
                     /locus_tag="PLS34_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006084032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP87846.1"
                     /translation="MLVRNAEATDWAAIAEFNRALAVETEDKVLDWDTLCNGVRRLLA
                     DPAKGRYFVAEADGRIVGTTMITYEWSDWRDGWIWWIQSVYVHPDFRGRGVFGRLYRH
                     VEALAAEGGVRAVRLYVLQSNRRARATYLALGMHESGYTVLEAGDGHGD"
     gene            3497..3572
                     /locus_tag="PLS34_10060"
     tRNA            3497..3572
                     /locus_tag="PLS34_10060"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3530..3532,aa:Ala,seq:cgc)
     gene            3679..>4550
                     /locus_tag="PLS34_10065"
     CDS             3679..>4550
                     /locus_tag="PLS34_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRP87847.1"
                     /translation="MDTAPETTRRVAMLGFEGLQILDVTGPLEVFSLASRLLLAQGRA
                     QIPPYEVMLAARSAGPVASSSGLALVADRSWNRLGPVDTLLVSGGAGVDRALEDRELL
                     AWLRREAPAARRYGSVCTGALLLARAGLLEGRRVTTHWAFLGALAEMVPAARIQPDAI
                     FLRDGRLWTSAGVTAGMDMALAMVEEDWGRTLALEVAQQLVMYLKRPGRHSQLSATLA
                     AQSAVAEGRFRDLMDWIFEHLEEDLSVAALAERMAMSPRHFSRSFAEETGLTPAKFVE
                     GARFEAARRMLTDG"
BASE COUNT          659 a         1482 c         1660 g          749 t
ORIGIN      
        1 ggtcgtgccg cgagacagtt cggccaccag ggtggcgacg gggaacagca tgagcctcgt
       61 gttgctttcc tgcatcactg tgccgttgag gcgggtgcgg atggccagcg actggggatc
      121 gccgatctgc ccggccggcg ccacgggacc gagggggcag aacgtgtcga aactcttgcc
      181 gcggcagaac tggccgccgc tgccttcctt ctgccaccag cgggcgctga cgtcgttggc
      241 gcaggcgtag ccgcgcacgt agtcgagtgc ccggctcgcc ggcacgtcac ggcagtcgcg
      301 cccgatgatc accgccagtt cgccctcgaa gtcgacctgc gggccgtgct cgcggcagat
      361 cggcggaatc acgatggcct cgccgtcgcc gaccacagag gcgggattct tcatgaacac
      421 cagcggccgg tcgggcgcgg cgctgcccat ctcggcggcg tgctcggcgt agttgcggcc
      481 gatggcgagg atggcggggg gaaggtcgcg gggcatcggg gtgcgttccg tcggctgcgg
      541 gtcgaacccg ggctttgttg cttcgggggt tcgaatcccg gtaaggcaat agcttcaatt
      601 tggcggagag ggggggattc gaacccccga acgggtcgcc ccgttgccgg ttttcaagac
      661 cggtgcattc aaccgctctg ccacctctcc gaggcggcga ttgtccccgc cgcgggtggg
      721 cgccgcaagc gtagccggcg gcgcgggtgc cgggcacggc ggtgagcgcc gggcggactt
      781 gaaccgctgc gcccgatggt cgagagtgta aggccggtgc gcaggaaagg cggcgatgca
      841 acagatcagg atccagttga cggggccggg cgccgggccg ggcggccatg gtggtcccgg
      901 gctggtggcg cgcatcctgt tgagcatcgg cgcggtgatc atgctggttt cggccgcgtt
      961 tctcggggcc attgtcttcc tggccgcgct cggtttcttc gtggtcgcga tgctggtgct
     1021 ggcggtgcgc atctggtggg ccaggcgaca acttgaaaag gccctgcgca gcggtcggcc
     1081 gcccggcgag ggcgggcccg ggcgacatca aggcgacatc gagggcgagt atcgtgtgct
     1141 ggacgagagg cgggatgcgc ccgccgagac gcgccggcgc gggggagatc gctgaggtgc
     1201 aggacctgaa aaaagaccac cgcgggttgc ggcgccggcc gctctacaca ccgctgacct
     1261 tcgtcgtcgg ctcggtgctc gcggtcctgg tgctggcggc gtggatcgtg gcctcctggg
     1321 gcagcaccac cgtggtgctg gtgcgccatg ccgagaaagt ggagctcggc gacgacccgg
     1381 gcctgtcgga agcggggctg gcccatgccc agcgtcttgc cgagatgctg gagcgcgccg
     1441 gcatcgaggc gatctacgtc agccaggccc ggcgcaccca ggaaaccgcg gcgccggtcg
     1501 ccgccctggc cggcgtggag ccgcgcgtgg tgcgcgccga ccagcacggc aagctgctgc
     1561 ggcggctgaa atggcggcat cgcagcgagg tcgtgctggt ggtcgggcac agcaacacgg
     1621 tgccggtcat cgccgacggg ctcggcgcgg agatcggcct ggtcgacgcc gaggattact
     1681 cgggactctg gatcatcagc tattcgcggc tgcgcggcac ccgtcttctg accttgcgtt
     1741 actagcggcc ggcgagcgcc agcaggtgcc ggccgtaggc ggccacatcg tcccagttcg
     1801 tgtagtcgat caccgccgtc ctgtcggtcg ggccgtgggt gaacttcatg atcatctgga
     1861 tggcgatcgt gtccagcggt ccccacgacg ggtaatcgac cttgccggcg atgaccttca
     1921 ggtcgcgcgg cttccaggcg gacaactcga gaaacttctg catgtagcgg ttgccctcga
     1981 cggtggcctt ctccggcgtg cgggccacca ccgagacatt gaagaagctg cttggcctgg
     2041 cttcgaggcg ggcctggttg gccgcgatga aatcgaacac cgatttgtcg taggtgccgt
     2101 acagcacggg tgcacccagc gcgatggcat cgtagcggtc ccagtcgacg ccctcgtgga
     2161 cggcctcgtt gatgtccatc atctcgcccc ggccgccggc ggactcgatg ctcgcgcaga
     2221 tcttgcgcgc gatgcgggcg gtgtggccgc cgcggctcat gtacaggacc aggacagatt
     2281 tcatggcgca gcctccgttg agcgcatctc cgccgctcat cgtgcacggt ccgtcggccg
     2341 cgagccatgc gggttttccc cagcggaggt aggatggccc ggtcgcccgg gcttgccccg
     2401 gggcgcgagc tgcgcgggga attgaacttg gtgacccagc aacggttgga aatcgcgaca
     2461 cggggcaggg gcacgaccgt gcctggcgag tgacttggcg ccgcgggacc ggaaccagcc
     2521 cgcgcgccta ggcggccgcg atcgccttgc ggatgaattc ctcgtgccgg ctgatcctca
     2581 ggtcggcaag caccgcatcg cgaatcacgc cgtcggcgcc gaccaggaag gtggcgcggc
     2641 gcacccccac gccgagcggg ccgtccacgc cccaggcctt cactgcggcc ttgccggggt
     2701 cggccagcag cgtgaagggc aactggtgct ggtcgcgaaa tcgcgcatga ctggtcgcat
     2761 cctgcgggct gatgccaacg acccgcatgc cggccgcggc gatctcgtcg tgcatgtcgc
     2821 ggatgctgca ggcttccttg gtgcagcccg gcgtgaaatc ggcgggatag aaatacagca
     2881 ccagcggccc gtcggcgagc aggttgcgca gcgataccgg cctgccgtcc tggtcgggca
     2941 gggtgaaatc aggcgcgtgg tccccggctt taagcatcat gtcgctccag gtggttcggc
     3001 tcgggcgaat tcagtcgcca tggccgtcgc cggcctcgag cacggtgtaa ccgctctcgt
     3061 gcatgccgag ggccaggtag gtcgcacggg cgcggcggtt gctttgcagg acgtagagcc
     3121 gcacggcacg gacgccgccc tcggcggcca gcgcttcgac gtgacggtag agacgcccga
     3181 acacgccccg gccgcgaaaa tccggatgca cgtaaacact ctggatccac cagatccagc
     3241 cgtcccgcca gtcgctccac tcgtaggtga tcatcgtcgt gccgacgatc ctgccatcgg
     3301 cctcggcgac gaaatagcgt cccttggcgg ggtcggccag cagccggcgc acaccgttgc
     3361 agagcgtgtc ccagtcgaga accttgtcct cggtctcgac cgccagggcg cggttgaact
     3421 cggcgatggc cgcccagtcg gtggcttcag cgttgcgaac gaggatgtcc atgcaataga
     3481 atgcgccgct tctcgtgggg ccgtagctca gttgggagag cgcctggatc gcacccagga
     3541 ggtcgtcggt tcgattccga tcggctccac cacttctcgc ctgaattctc aacgcattat
     3601 ccccgtcccg ggcctgaatg gacagggcga gtccctgtcg cagtatcggc cccaggccgg
     3661 ccggcgagca cgcccctgat ggacaccgct cccgagacca cccgccgcgt cgccatgctg
     3721 ggcttcgagg ggctccagat tctcgacgtc acggggccgc tggaggtctt ttcgctcgcc
     3781 tcccgcctgc tgctcgcaca gggacgggcc cagatcccgc cctacgaagt gatgctggcg
     3841 gcgcgtagcg ccggtccggt ggccagttcc tcgggactcg cgctggtggc ggaccgcagc
     3901 tggaaccggc tcgggccggt ggataccttg ctggtatccg gcggtgccgg cgtggaccgg
     3961 gcactcgagg accgtgagct gctcgcctgg ctgcgccgcg aggcgcccgc ggcgcggcgc
     4021 tacggctctg tctgcaccgg cgcgctgctg ctggcgcgcg ccgggctgct ggaggggcgg
     4081 cgggtcacca cgcattgggc cttcctgggc gcgcttgcag agatggtgcc ggcggcccgc
     4141 atccagccgg acgcaatttt cttgcgcgac gggcgcctgt ggacctccgc cggcgtcacc
     4201 gcggggatgg atatggcact ggcgatggtg gaggaggact ggggccgaac gctggcactc
     4261 gaagtggcac agcagctggt catgtacctg aaacggcccg gccggcactc gcagctcagc
     4321 gccacgctgg cggcgcaatc ggcggtggcc gaggggcgct ttcgggatct catggactgg
     4381 attttcgaac atctcgaaga agacctgtcc gtggcggccc tggcggagcg catggccatg
     4441 agtccgcgcc atttcagccg gagctttgcc gaggagacgg gcctgacgcc ggccaagttc
     4501 gtcgaggggg cccgattcga ggcggcgcgg cggatgctca ccgacggcga
//
LOCUS       DAOMPG010000196         4532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7984_length_4532_cov_4.608443, whole genome shotgun sequence.
ACCESSION   DAOMPG010000196 DAOMPG010000000
VERSION     DAOMPG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_4532_cov_4.608443"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLS34_10070"
     CDS             <1..463
                     /locus_tag="PLS34_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HRP87848.1"
                     /translation="TNDQVLTDVYHSDLRRARAAAMSMIPTKTGAAAAVGLVLPELAG
                     KLDGFAIRVPTINVSMVDLTFQAARDTTKEEIDRVLEEASQGRLKNILGFNREPLVSI
                     DFNHDPRSSVYESSLTRVIDGRLVKVCSWYDNEWGFSNRMLDTTIALVNAK"
     gene            472..1650
                     /locus_tag="PLS34_10075"
     CDS             472..1650
                     /locus_tag="PLS34_10075"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRP87849.1"
                     /translation="MHVIRMQDLELAGRRVLIREDLNVPVEGGRVSSDARLRAALPTI
                     RMALNMGAAVLLMSHLGRPKEGEFDPQYSLAPVAARLGELLGKPVRLERDWLDGVEVA
                     PGEVVLCENVRFNAGEKKDDETLARRMAALCDVFVMDAFGTAHRAQASTHGVARFASV
                     ACAGPLLVAELEALGKALREPARPLVAIVGGSKVSTKLTVLESLLDRVDQLIVGGGIA
                     NNFIAAAGFPVGRSLHEPDLLMETRTLVAKAEARGAAIPLPRDVVVAAGLEPGQAARV
                     VPVDKVGEHDMILDVGPETAAAYADILARAGTIVWNGPVGVFEDQRFAAGTRALAKAV
                     AASTAFSIAGGGDTLAALDAFGLSDQVSYVSTGGGAFLEFLEGRTLPAVAMLEQRARS
                     "
     gene            1658..3085
                     /gene="pyk"
                     /locus_tag="PLS34_10080"
     CDS             1658..3085
                     /gene="pyk"
                     /locus_tag="PLS34_10080"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRP87850.1"
                     /translation="MAKRTKIVATLGPATDRPGVLEAMLRAGANVVRLNFSHGNAEDR
                     RRRVAAVREAAHRAGRDIGILGDLQGPKIRVERFAAGPVELVDGDAFALDASLAADAG
                     DRHGVGIAYKDLPKDVSPGDILLLNDGAITLEVERVAGPRVDCRVRVGGVLSDGKGIN
                     RLGGGLSAGALTDKDREDIRAAAELGLDFLAVSFPRCAADMEEAGRLLLEAGGNALLV
                     AKIERAEAVARIEEIVHASDVVMVARGDLGVEIGFAEVPGVQKNIIRVARAMNRVVIT
                     ATQMMESMISEPIPTRAEVSDVANAVLDGTDAVMLSGETAVGRHPVKAVEAMASACIA
                     AEKQRATSISHHRMDDVFERTDEAIAMATMYTANHLDVKAIVALTESGSTALWMSRIR
                     SGIPIYAFSRSEATRRRVALYRGVYPVSFDVLADGGSEGFAAICHALKDRGLAAAGDR
                     ILLTHGHSAGVAGGTNTMKILTVDG"
     gene            complement(3107..>4532)
                     /locus_tag="PLS34_10085"
     CDS             complement(3107..>4532)
                     /locus_tag="PLS34_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRP87851.1"
                     /translation="DPVATAVILSALSGLILLAMGILRMGVFANLLSQPVLTGFSSGA
                     AIVIMADQLRHFAGIELPAGLQPHEMLAYLGGRAAILNLQTLLLGGLALALLLVASGT
                     TRGGRRLNAVTARLAPVIVLAAGAIAVAALGLERVPLVGTITARPPILDATMPGPAAW
                     VALLPSAALISIIVFVESVSISKYLAGRRREAVDADQELVALGAANVGSALSGALPVA
                     GSFSRSMVSYNSGGRSQLSSIFLAVFTGATLLAFAPAFSGLPKAVLAAVIIVAVAGLV
                     DLRSIPDTWRYSKADGASNLVTFVGVLAYGVEAGLVMGVLLSVLLYLWRTGHPNIAVV
                     GRLPHSTEFRSIERHKVETWPEILLVRVDENLYFANVGHVQDMITRELQRRPGIKHLV
                     LVMSGVGFVDSSALKVMQVAVDTLRESGVTIHLADVKGPVLDRLRRTKIFEQMAPGQL
                     FPTPQMAVTALSRAAEREKELIPL"
BASE COUNT          722 a         1551 c         1600 g          659 t
ORIGIN      
        1 caccaacgac caggtgctga ccgacgtcta ccactccgac ctgcgccgcg cgcgcgccgc
       61 cgccatgtcg atgatcccga ccaagaccgg cgccgccgcc gccgtcggcc tcgtgctgcc
      121 ggagctcgcc ggcaagctcg acggcttcgc cattcgcgtg ccgaccatca acgtctcgat
      181 ggtggacctg acgttccagg ccgcgcgcga cacgacgaaa gaagagatcg accgggtgct
      241 cgaggaggcg tcgcagggcc gcctgaagaa catcctcggc ttcaaccgcg agccactggt
      301 gtcgatcgac ttcaaccacg acccgcgttc ctcggtgtac gagtcttcgt tgacgcgggt
      361 catcgacggc cggctggtga aggtctgctc ctggtacgac aacgagtggg gcttctccaa
      421 ccggatgctc gacaccacca tcgcgctggt caacgcgaag taaggcgcgg catgcacgtc
      481 atccgcatgc aggacctgga gcttgccggc cggcgggtgc tgatccgcga ggacctgaac
      541 gtaccggtag agggcggcag ggtgagcagc gacgcgcgcc tgcgcgcggc gctgcccacc
      601 atccggatgg cgctgaacat gggcgctgcg gtgctgttga tgtcgcacct cggccggccg
      661 aaagagggcg agttcgaccc gcagtattcg cttgcaccgg tggcggcgcg ccttggagaa
      721 ctgctcggga aaccggtgcg cctggaacgc gactggctgg acggcgtcga ggtcgcgccg
      781 ggcgaggtcg tgttgtgcga gaacgtgcgc ttcaacgccg gcgagaagaa ggacgacgag
      841 accctcgcca ggcgcatggc ggcgctgtgc gacgtgttcg tgatggacgc cttcggcacg
      901 gcccaccggg cacaggccag cacgcacggc gtggcgcgct tcgcgtccgt ggcctgcgcc
      961 gggccgctgc tggtggcgga gctcgaggcg ctcggcaagg cgctgcgcga gcccgcccgg
     1021 ccgctggtcg ccatcgtcgg cggctccaag gtctccacca agctgaccgt gctggagtcc
     1081 ctgctcgatc gcgtcgacca gctcatcgtc ggcggcggca tcgccaacaa tttcatcgcc
     1141 gcggccggat tcccggtcgg caggtcgctg catgaaccgg acctgttgat ggaaacgcgc
     1201 acgctggtgg ccaaggccga ggcgcgcggg gcggccattc cgctgccgcg cgacgtggtg
     1261 gtagcggccg ggctggagcc cggccaggct gcccgcgtgg tgccggtcga taaagtcggc
     1321 gaacacgaca tgatcctgga cgtcgggccg gaaacggctg ccgcctacgc ggatatcctg
     1381 gcgcgcgccg ggaccatcgt ctggaacggt ccggtcggcg tattcgagga ccagcgcttc
     1441 gccgccggca cgcgcgcgct ggccaaggcg gtcgccgcga gcacggcctt ctcgatcgcg
     1501 ggcggcggcg acacgctggc ggcgctggac gccttcgggc tcagcgacca ggtttcttat
     1561 gtctccaccg gcggcggcgc cttcctggaa tttctcgagg gccgcacgct gccggccgtg
     1621 gccatgctcg aacagcgcgc ccgcagctga gcgcccggtg gccaagcgca ccaagatcgt
     1681 tgcgaccctg ggcccggcca ctgatcggcc gggcgtcctg gaggccatgc tgcgggccgg
     1741 cgccaacgtc gtccgcctga atttttccca cggcaacgcc gaggaccgcc ggcggcgggt
     1801 cgcggcagtg cgcgaggcgg ctcatcgcgc cggcagggat atcggcattc tcggcgacct
     1861 gcagggaccg aagatacgcg tcgagcggtt cgccgcaggc ccggtcgaac tggtcgacgg
     1921 tgatgccttc gcgctggatg cctcgctggc ggcggacgcg ggcgaccggc atggcgtagg
     1981 catcgcctac aaggacctgc cgaaggacgt cagtcccggc gacatcctgt tactcaacga
     2041 cggcgccatc acgctggagg tcgagcgtgt cgcggggccg cgggtcgatt gccgcgtccg
     2101 cgtgggcggc gtgctgtccg acggcaaggg aatcaaccgc ctgggcggcg ggctctccgc
     2161 cggcgccctg accgacaagg accgggagga catccgcgcc gcggccgagc tcggcctgga
     2221 cttcctggcg gtgtcgtttc cgcgctgcgc ggcggacatg gaggaggcgg gccgcctgtt
     2281 gctcgaggcg ggtggaaacg ccctgctggt ggcaaagatc gagcgggccg aggccgtcgc
     2341 gcgcatcgag gagatcgtgc atgccagcga cgtggtgatg gtggcgcgcg gtgatctcgg
     2401 cgtggaaatc ggcttcgccg aggtgcccgg cgtgcagaag aacatcattc gcgtggcccg
     2461 ggcgatgaat cgcgtcgtca tcacggcgac gcagatgatg gagtcgatga tcagcgagcc
     2521 gatcccgacc cgcgccgagg tttccgacgt ggcgaatgcg gtgctcgacg ggacggatgc
     2581 cgtgatgctg tccggcgaga ccgcagtcgg ccgtcacccc gtcaaggcgg tggaggccat
     2641 ggccagcgcc tgcatcgctg ccgaaaagca acgtgccacc tccatctccc atcaccgcat
     2701 ggacgacgta ttcgagcgca ctgacgaagc cattgcgatg gccacgatgt acacggccaa
     2761 tcacctcgac gtgaaggcga tcgtcgcgct gaccgaatcc ggcagcaccg cgctgtggat
     2821 gtcgcggatc cgttccggca tcccgatcta tgccttcagc cgcagcgagg cgacgcgccg
     2881 gcgcgtcgcg ctctatcgcg gcgtctatcc cgtgtccttc gacgtgctgg cggacggcgg
     2941 ctcggaaggg ttcgcggcca tctgtcacgc gctcaaggat cgcggcctcg cggcggcagg
     3001 cgaccgcatc ctgctcactc acgggcattc cgccggcgtc gccggcggca ccaatacgat
     3061 gaagatactc acggtcgacg gctgaggctc cggtcgcggc ccggcctcag agcggaatca
     3121 attccttctc gcgttccgcc gcgcggctca gcgccgtgac cgccatctgc ggcgtgggga
     3181 acagctgccc cggcgccatc tgctcgaaga tcttggtgcg ccgcaaccgg tcgagcaccg
     3241 gacccttgac gtcggcaagg tgaatcgtca cgccggattc gcgcagcgtg tcgaccgcga
     3301 cctgcatcac tttcaacgcg ctcgaatcca cgaagccgac gccgctcatc accagcacca
     3361 ggtgcttgat gccgggccgc cgctggagct cgcgcgtgat catgtcctgg acgtggccga
     3421 cgttggcgaa atagaggttc tcgtcgacgc gcaccagcag gatctccggc caggtctcga
     3481 ccttgtgccg ctcgatgctg cgaaactcgg tggaatgcgg cagccgtccg acgaccgcga
     3541 tgttcgggtg gccggtgcgc cacaggtaga gcagcaccga cagcagcacg cccatcacca
     3601 gcccggcctc gacgccgtag gcgagcacgc cgacgaaggt caccaggttc gaggccccgt
     3661 ccgccttgct gtagcgccag gtatcgggga tcgagcgcag gtccaccagc ccggctacgg
     3721 ccacgatgat caccgccgcg agcaccgcct tgggcaggcc gctgaaggcc ggcgcaaaag
     3781 cgagcagggt tgcgccggtg aagacggcaa gaaaaatcga cgacagctgg ctgcgcccgc
     3841 ccgagttgta gctcaccatg ctgcggctga agctgccggc gaccggcagg gctcccgaca
     3901 gcgccgagcc gacgttggcc gcccccagcg cgaccagttc ctggtcggca tccactgctt
     3961 cgcggcgcct gccggcgagg tacttgctga tgctgacgct ttcgacgaac acgatgatgc
     4021 tgatcagcgc ggccgaaggc agcagcgcca cccaggcggc cgggcccggc atcgtggcgt
     4081 cgaggatcgg cgggcgggcg gtgatcgtcc ccaccagcgg cacgcgctcg agcccgaggg
     4141 cggccacggc gatcgccccg gccgccagca cgatcaccgg agccagccgg gcggtcaccg
     4201 cgttgagacg ccgtccgccg cgcgtcgtac cgctggccac cagcagcaac gccagcgcca
     4261 ggccgcccag cagcagcgtc tggaggttga gaatcgcggc gcgcccgccg agataggcga
     4321 gcatttcatg cggctgcagg ccggccggca gctcgatgcc ggcgaaatgc cgcagctggt
     4381 cggccatgat gacgatcgcc gcaccgctgg agaaaccggt cagcaccggc tggctcagca
     4441 ggttggcgaa cacgcccatg cggaggatgc ccatggccag caggatgagc ccggacagcg
     4501 cggacaggat cacggcggtg gccaccgggt cg
//
LOCUS       DAOMPG010000197         4530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_7988_length_4530_cov_3.898771, whole genome shotgun sequence.
ACCESSION   DAOMPG010000197 DAOMPG010000000
VERSION     DAOMPG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7988_length_4530_cov_3.898771"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..>1531
                     /locus_tag="PLS34_10090"
     CDS             137..>1531
                     /locus_tag="PLS34_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRP87852.1"
                     /translation="MLQKIREKITGWVAWAIVITIGVVFAVWGIDLSFTPRPMAAKVN
                     GAEVPVEPVRRAYQEQAARFQQAFRGELPEEIAAEIRRGVIEQFVRRELLQQRVVQQG
                     YRISDAALTEYVQSYDVFHVGGRFNMETYRAALAGAGYTPAMFEAEQRRMLEIQQLQD
                     AVVLSSFVTEDELERRVALQRELREIEWIAVPLARFAEEVEVSDADVEARYQATPERW
                     MTPESVDIAYIDLQLDSLAGDVQVSEDDLRAFYETEMTREPERYAGRERRKAAHILVR
                     PGDDAAAAEARVRALRERIVAGEDFAAVATEASEDPGSARMGGELGWVERGMMVPAFE
                     EALFAMEAGTVSEPVRSEFGWHLIRLEEIEQIGGSSFEAAREELLQAYARRRAEDRFY
                     DDAEALARIAFENPDSLEPAATELGYPVQELAGVTRRGGPGIGTNPAVIDAAWSEAVF
                     ERRENSRLLELDDGH"
     assembly_gap    1532..1590
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1591..2020
                     /locus_tag="PLS34_10095"
     CDS             <1591..2020
                     /locus_tag="PLS34_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87853.1"
                     /translation="GAEIRQERPAERAAELGEQARARLAAGESIAEVAESLSADFVAG
                     ITVVRDDETVPPDVARAAFSAPRPVGGQPSVVAAQGANAHFAVRVLSATPGGLELLRP
                     EERRELNDRVRGARASQELQAYLEHLRQGAKVTVFENALQ"
     gene            2108..3388
                     /locus_tag="PLS34_10100"
     CDS             2108..3388
                     /locus_tag="PLS34_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRP87854.1"
                     /translation="MRKLQLHWQILIAILVGIVAGWLSGDEGALFGITLYSVYDFVGT
                     LFINALRMLIVPLIVSSIIVGVAGIGSGGNLGRLGGKTLMFYGVTTLLAILIGLVLVN
                     IVQPGIVNGEPARELLALEESTRAVAESVGVAAGAGGGGQLVQMFLSMVPPNIIQAAA
                     DGQMLGLIFFSIVFGYFMTRVGHEKGDAMYRFWDALFAVMMKITHWVMAFAPIGVFGL
                     VARTVARTGFDAAQPLIVFSLTVLAALLLHAILTIPLLIKLVARASPVALYRAAAPAL
                     LTAFSTASSAATLPITMDSMERRAGVSNRTSSFVLPLGATVNMNGTALYECVAAMFIA
                     QAYGLELSFGIQFIIVVTALVTSVGVAGIPSASLVAIAIILAAVGLPVEAIGVLFVFD
                     RILDMARTAINILGDCACAVIIAVLDGEEGVLQG"
     gene            complement(3408..>3937)
                     /locus_tag="PLS34_10105"
     CDS             complement(3408..>3937)
                     /locus_tag="PLS34_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRP87855.1"
                     /translation="LQENLSRNRADAVVVAADAVGESTGAAELLRYGHRRTNGGLLPV
                     GAAEKVSVSMVSLDDYCREHELERRPVGLIKIGIEGFEYFALRGAERTLVRCRAVLAE
                     YAPERLAQAGIEPVALLDLLVAAGFIPTVLDAAGPREVTRDELLAGRGRRHLFWSRPA
                     GLAAPPAPDPRALAI"
     assembly_gap    3938..3971
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3972..4272)
                     /locus_tag="PLS34_10110"
     CDS             complement(<3972..4272)
                     /locus_tag="PLS34_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87856.1"
                     /translation="MLRSAAFFVQRFVLGRRTLLAQVPEFDLELRVPAGDAVGRHLYE
                     DAMHAPAVIDFLATRLELAPDDVVFDIGANIGWYALLLARLAPRGVSIHAFEPDPW"
     gene            complement(4283..>4530)
                     /locus_tag="PLS34_10115"
     CDS             complement(4283..>4530)
                     /locus_tag="PLS34_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP87857.1"
                     /translation="GLRVNGISAGPIKTLAAAGIGGFRKMLAQVARIAPLQRNVTIED
                     VGNAAAFLCSDLAAGITGEILYVDGGYSHVGMSLPED"
BASE COUNT          654 a         1507 c         1586 g          690 t
ORIGIN      
        1 aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc cgtccgctcc gccaatgaac
       61 gaaagggcgc ccgtgtggcg ccctttcact ttccggaccc gcccggccgg gtccgacagg
      121 acattcgagg caggccatgc tgcagaaaat ccgcgagaag atcaccggct gggtcgcctg
      181 ggcgatcgtc atcaccatcg gcgtcgtgtt cgccgtgtgg ggcatcgacc tgagcttcac
      241 gccacgcccg atggccgcca aggtcaacgg cgcagaggtg ccggtcgagc ccgtgcgccg
      301 cgcctaccag gagcaggcgg cccgcttcca gcaggcgttc cgcggcgaat tgccggaaga
      361 gatcgcggcc gagatccgtc gtggcgtcat cgagcagttc gtccgtcgcg agctgttgca
      421 gcagcgcgtc gtgcaacagg gctatcgcat cagcgacgcc gcgctgaccg aatacgtgca
      481 gtcctacgac gtgttccatg tcggcggccg gttcaacatg gaaacgtacc gcgcggcgct
      541 ggctggtgcc ggctacacgc cggccatgtt cgaggccgag cagcgccgga tgctcgagat
      601 ccagcagctg caggacgcgg tggtgctgtc gtcgttcgtg accgaggacg agcttgagcg
      661 acgcgtggcc ctgcagcgcg agctgcgcga gatcgagtgg atcgccgtgc cgctggcgcg
      721 cttcgccgag gaggtcgaag tcagcgatgc cgacgtcgag gcccgctacc aggccacgcc
      781 ggagcgctgg atgaccccgg agagcgtcga catcgcctac atcgacctgc aactcgacag
      841 cctcgccggc gacgtgcagg tttcggagga cgacctgcgc gccttctacg agacggaaat
      901 gacccgcgag ccggagcgct acgcgggccg cgagcgccgc aaggccgccc acatcctggt
      961 gcgcccgggc gacgacgccg cggcggcgga agcgcgggtg cgtgccctgc gcgagcggat
     1021 cgtggccggc gaggactttg ccgcggtggc caccgaggcg tcggaggacc cgggctcggc
     1081 gcgcatgggc ggtgagctcg gctgggtcga gcgcggcatg atggtgcccg cgttcgagga
     1141 agcgctgttc gccatggagg ccggcacggt cagcgagccg gtgcgcagtg aattcggctg
     1201 gcacctgatc cgcctcgagg agatcgagca gatcggcggt tccagtttcg aggccgcgcg
     1261 cgaggagctg ctgcaggcct atgcgcggcg ccgtgccgag gatcgctttt acgacgacgc
     1321 cgaggcgctg gcccggatcg ccttcgagaa cccggacagc ctcgagccgg cggcgaccga
     1381 gctcggttac cccgtgcagg agctcgccgg cgtgacgcgc cgcggcggac cgggcatcgg
     1441 caccaaccct gccgtaatcg acgcggcctg gagtgaggcg gtcttcgagc gccgcgagaa
     1501 cagccgcctg ctggagcttg acgacggcca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtgcggag atccgccagg aacggcccgc
     1621 cgagcgtgcc gccgagctgg gcgagcaggc gcgcgcccga cttgcggccg gcgagtcgat
     1681 cgccgaggtc gccgagtcac tgtccgcgga cttcgtcgcc ggcatcaccg tggtgcgcga
     1741 cgatgagacg gtgccgccgg acgtggcgcg ggccgcgttc agcgcgccgc gtccggtggg
     1801 cggccagccg tcggtggtgg ccgcgcaggg tgcgaacgcg cacttcgcgg tgcgggtgct
     1861 gtcggcgacg ccgggcgggc tcgagctgct gcggcccgag gagcgccgcg agctcaacga
     1921 ccgcgtgcgc ggtgcgcgcg cctcgcagga gctgcaggct tacctggagc acctgcgcca
     1981 gggcgccaag gtcaccgtgt tcgagaacgc gctgcagtag ctgcaggaaa cggggtcaga
     2041 caccaatttc cgtcggaaat tggtgtctga ccccgtttcc cacggtttta cgcgttaaca
     2101 tcgcacgatg cgcaagctcc agcttcactg gcagatcctg atcgcgatcc tggtcggcat
     2161 cgtcgcgggc tggctcagcg gcgacgaggg cgcgctgttc ggtatcacgc tctacagcgt
     2221 ctacgacttc gtcggcacgc tgttcatcaa cgccttgcgc atgctgatcg tgcccctgat
     2281 cgtctcgtcg atcatcgtcg gcgtggccgg catcggcagc ggcggcaacc tcggtcgtct
     2341 cggcggcaag acgctgatgt tctacggcgt gaccacgctg ctggcgattc tcatcggcct
     2401 cgtgctggtg aacatcgtgc agccgggcat cgtcaacggc gagccggcac gcgaactgct
     2461 ggcgctggag gagagcacgc gcgcggtggc cgagagtgtc ggcgtggcgg cgggcgccgg
     2521 cggcgggggg cagctggtgc agatgttcct cagcatggtg ccgcccaaca tcatccaggc
     2581 ggcggccgac ggacagatgc tcgggctgat tttcttcagc atcgtgttcg gctatttcat
     2641 gacgcgcgtc ggccacgaga agggcgacgc gatgtatcgc ttctgggacg cgctgttcgc
     2701 cgtcatgatg aagatcaccc actgggtgat ggccttcgcc ccgatcggcg tcttcgggct
     2761 ggtggcgcgc accgtggccc gcaccggctt cgacgccgcg cagccgctga tcgtgttcag
     2821 cctgacggtg ctggccgcgc tgctgctgca cgcgatactg accatcccgc tgctcatcaa
     2881 gctggtggcg cgcgccagcc cggtggcgct gtatcgcgcg gcggcgccgg cgctgttgac
     2941 ggccttctcg acggcgtcct cggccgccac gctgcccatc accatggact cgatggagcg
     3001 ccgcgcgggg gtctccaacc gtacctcgag cttcgtgctg ccgctgggcg ccaccgtcaa
     3061 catgaacggc accgcccttt acgagtgtgt cgcggcgatg ttcatcgcgc aggcctacgg
     3121 gctggaactg tccttcggca tccagttcat catcgtcgtc acggcgctgg tgacctcggt
     3181 gggcgtggcc ggcattccct ccgcctcgct ggtcgcgatc gcgatcatcc tcgccgcggt
     3241 cggcctgccg gtggaagcga tcggcgtgct gttcgttttc gaccgcatcc tcgacatggc
     3301 gcgcactgcc atcaacatcc ttggcgactg cgcctgcgcc gtgatcatcg cggtgctcga
     3361 cggcgaagag ggcgtgctgc agggctgaat tggggacatt cccctgttca gatcgccaac
     3421 gcccgggggt cgggcgccgg cggcgcggca agccctgcgg ggcggctcca gaacaggtgg
     3481 cgccggccgc gcccggccag cagttcgtcg cgggtgactt cgcgcggccc ggccgcatcg
     3541 agcaccgtcg ggatgaagcc cgcggccacc agcaggtcga gcaacgccac cggttcgatg
     3601 ccggcctgcg ccagccgctc gggcgcgtac tcggcgagca ctgcgcgaca gcgcaccagc
     3661 gtgcgctcgg cgccgcgcaa cgcgaagtac tcgaagccct cgatgccgat cttgatcaag
     3721 ccgaccgggc gccgctcgag ttcgtgctcg cgacaatagt cgtccaggct caccatcgag
     3781 accgacacct tctccgctgc accgaccggc agcaatccgc cgttggtccg ccgatggccg
     3841 tagcggagca gctccgcagc cccggtggac tccccgacgg cgtcggccgc caccaccacg
     3901 gcgtcggcgc ggttgcggct gaggttttcc tgcagcannn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn ncccagggat ccggctcgaa ggcatggatc gagacgccgc gcggcgcgag
     4021 ccgcgccagc agcagcgcat accagccgat gttggcgccg atgtcgaaca ccacgtcgtc
     4081 cggtgcgagt tcgaggcgcg tggcgagaaa gtcgatgacc gccggcgcgt gcatggcatc
     4141 ctcgtacagg tgccggccga cggcgtcgcc cgccggcacc cgcaattcga ggtcgaactc
     4201 cggcacctgg gccagcaggg tgcggcggcc cagcacgaaa cgctgcacga agaaggcggc
     4261 ggagcgcagc atccgggcgt cgtcagtctt ccggcagcga catgccgacg tgactgtagc
     4321 cgccgtccac gtacaggatc tcgccggtga tgccggcggc caggtcggag cacaggaagg
     4381 cggcggcgtt gccgacgtcc tcgatggtga cgttgcgctg caggggtgcg atgcgcgcca
     4441 cctgcgcgag catcttgcgg aagccgccga tgccggcggc cgccagcgtc ttgatcggcc
     4501 cggccgagat gccgttgact cgcaggccct
//
LOCUS       DAOMPG010000198         4518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8021_length_4518_cov_3.225857, whole genome shotgun sequence.
ACCESSION   DAOMPG010000198 DAOMPG010000000
VERSION     DAOMPG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_4518_cov_3.225857"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLS34_10120"
     CDS             complement(<1..339)
                     /locus_tag="PLS34_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRP87858.1"
                     /translation="MSVSLEALRARARPIPLDADARCAWAEQFNAMPAMSATGAALDL
                     EDPALVRVHLSEVGPHHRGGMGSSAVNGAVIAGLFDAALGVAGVLQFAGRRAGTVELS
                     VKFMRPTRGRS"
     gene            477..1322
                     /locus_tag="PLS34_10125"
     CDS             477..1322
                     /locus_tag="PLS34_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRP87859.1"
                     /translation="MDSDRHALQDQPAAPADEGPSPGGIGQTVIDRLDRCVERLENPE
                     LHPGWVVHEVRKDLKRVRALLRLCGDELPTRKLEKRCAAAARELAHLRDAAALGETLA
                     RLRTRADARQLEALEAVIESLGLERPPGQPHGLPRPVADDAAATLRKVRKHCAGLDFA
                     RMDAAALDAGLAESWSDTALAFHRVVERPALPRFHEFRKAVKRELHQRELSGRPLDRM
                     ERATLKKLADVLGELQDLDVLRERLRAAGRWRGPVRKLVKQTIRELKARAVRLGVGRY
                     TEGSS"
     gene            1360..2037
                     /locus_tag="PLS34_10130"
     CDS             1360..2037
                     /locus_tag="PLS34_10130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP87860.1"
                     /translation="MIPLRDDNPVHDTPVVTWALVASCVLVFLWQISLGEAGFNRVVF
                     TLGVIPAVLLGNAQLPPEVAVVPPTATMLTSMFLHGGWLHLAGNMLYLWIFGDNIEDR
                     MGRLRFLLFYLACGIAAVLAQALPEPQGRTPMIGASGAISGVLGAYLLLFPRARVLVL
                     VPLGFILKAIRLPAVWVLGLWFLVQLVASLMAPAGEGGVAFRAHLGGFVAGLAFAPLF
                     LLGRRRS"
     gene            2034..2186
                     /locus_tag="PLS34_10135"
     CDS             2034..2186
                     /locus_tag="PLS34_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87861.1"
                     /translation="MNDWLKLMLEEIRRKQREREQASREHQRRGSGPGAPAPTGPRSP
                     GAGRRD"
     gene            2200..3156
                     /locus_tag="PLS34_10140"
     CDS             2200..3156
                     /locus_tag="PLS34_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP87862.1"
                     /translation="MSSSANAPLYGALRQGPAEWGLLLALVALWGSSFLLVRVSLDAF
                     TPVAVTAGRLLIGAGVLVLLLVFSGRRPPGGVRTWLFFTAMAIIGNALPFFLIAWGQR
                     DIPSGLTGILMAVMPLVVLVLAHFLVVGERLTPRRLAGFTLGFLGVVVLTGPEARAAL
                     VGREASFIAQLAVLGAAVCYGIAAIIARRGPVLKPVVTAAAVLSIAGIVVGAVLLARG
                     LREGEAAVAHATIATGPALALLALGALSTGLATVVYFSLVARAGPTFLSLINYLIPVW
                     AVALGAMALGERLPTRAFAALALILAGVVLARWQFRGRRLPA"
     gene            3153..4250
                     /locus_tag="PLS34_10145"
     CDS             3153..4250
                     /locus_tag="PLS34_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP87863.1"
                     /translation="MKIVVVGGGYAGLACLARLARVLPDAERHLVEPNRWHLRRTLLH
                     ETLRRPLADLRADLAPVARRWGFRHHRARIAWTAERLVEWSRAGRLPVGRRELEFDAL
                     VVTTGLPGLAGRVSRSRTPPIPLEALATGPGVNAWKRLAASADRVWVVGAGASGLQCL
                     FELAAARPRGAPLGLVEASDTILASEPDGLRREVLERLDALHLDLRLGTRYDGFGRGR
                     VRMQGPAGALSEPAAGVLLCTGPTVQRIDANAAGQVLHRRRPLPRVFAAGDCARWHGV
                     PFDAATAQTAVRKGRQVAETVARVAAGRAPDDWDARQLGFFLSLGPHDAIGWVFARAA
                     LVSGLPAVAAREAVEARWDLLLRGIDSFAAF"
BASE COUNT          514 a         1605 c         1677 g          722 t
ORIGIN      
        1 ggagcggccg cgggtcggcc gcatgaactt gacggacagt tccaccgtgc cggcgcgccg
       61 ccctgcgaac tggaggacgc ccgccacccc gagcgcggcg tcgaacagcc cggcgatgac
      121 cgccccattg accgcgctgc tacccatgcc gccgcggtga tgcggcccca cctcggaaag
      181 atgcacccgg accagcgccg ggtcctcgag atccagcgcc gcgccggtcg cgctcatggc
      241 gggcatggca ttgaactgct cggcccaggc gcagcgggcg tcggcgtcca ggggaatcgg
      301 tcgggcgcgg gcgcggagcg cttccaggga gacggacatc gggttcattt acaggcaccg
      361 gtcggctcgg aggtggtttc aggatacccc cgatgttgca tcgccgcacg acctgcggcg
      421 gtgctagaat gactttcccg acccaacccg acccaacccg acccccggac cctcgaatgg
      481 actcggacag gcacgcgcta caggaccagc ccgcggcacc ggcggacgaa ggcccctcgc
      541 cgggcggtat cggccagacc gtgatcgacc ggctcgaccg gtgcgtcgag cgcctcgaaa
      601 atcccgagct gcaccccggt tgggtggtgc acgaggtgcg caaggacctc aagcgggtcc
      661 gggcgctgct gcggctgtgc ggcgacgaac tgccgacgcg caagcttgag aagcgctgcg
      721 ccgccgccgc gcgcgagctg gcgcacctgc gtgacgccgc cgcgctcggc gagaccctgg
      781 cgcgcctacg tactcgcgcc gacgcccggc agctggaggc gctcgaggcg gtgatcgagt
      841 cgctgggact cgagcgcccg cccggccagc cacacggcct gccgcgaccc gtcgcggacg
      901 acgcggcggc cacgctgcgc aaggtgcgca agcattgcgc cgggctcgat ttcgcgcgca
      961 tggacgccgc tgcgctggat gccgggctgg ccgagtcatg gtcggatacc gcgctggctt
     1021 tccaccgcgt cgtggagcgg ccggcgctgc cgcgtttcca cgaattccgc aaggccgtga
     1081 agcgtgagct gcaccagcgc gagctgtcgg gccggccgct ggaccgcatg gagcgtgcca
     1141 ccctcaagaa gctggcggac gtgctcggcg aactgcagga tctcgacgtg ttgcgcgagc
     1201 ggctgcgtgc cgccgggcgc tggcgtggcc cggtgcgcaa gctggtcaag cagaccatcc
     1261 gcgaactcaa ggcgcgcgcc gtgcggctgg gcgtcggccg ttacacggag ggatcgtcct
     1321 gagccatccg gcgggccgcc acgacgttcc gggccgggca tgattccgct gcgcgacgac
     1381 aaccccgtgc atgacacgcc ggtcgtgacc tgggcgctag tcgcatcctg cgtgctggtg
     1441 ttcctctggc agatttcgct cggtgaggcc ggattcaacc gcgtggtgtt caccctcggc
     1501 gtgatccccg ccgtgctgct gggcaatgcg cagctgccgc cggaggtcgc cgtggtgccg
     1561 ccgaccgcga ccatgctgac ctcgatgttc ctgcacggcg gctggctgca cctggccggc
     1621 aacatgctgt acctgtggat cttcggcgac aacatcgagg accgcatggg gcggctgcga
     1681 ttcctgctgt tctacctggc ttgcggcatc gccgcggtgc tggcgcaggc gctgccggag
     1741 ccgcaggggc gcacgccgat gatcggtgcc tcgggcgcga tctccggtgt gctcggcgcc
     1801 tacctgctgc tgttcccgcg tgcgcgcgtg ctggtgctgg tgccgctggg ctttatcctg
     1861 aaggccatcc gcctgccggc ggtctgggtg ctcgggctgt ggttcctggt gcagctcgtc
     1921 gcctcgctga tggcgccggc gggcgagggc ggcgtggcgt tccgggcgca cctgggcggc
     1981 ttcgtggcgg gcctggcgtt cgcgccgctg ttcctcctgg ggagacgacg atcgtgaacg
     2041 actggctgaa gctgatgctg gaagaaattc gccgcaagca gcgcgagcgc gagcaagcgt
     2101 cgcgcgagca ccagcggcgc gggtccggcc ccggtgcgcc ggcgccgact gggccccgat
     2161 cgcccggcgc cggcaggcgc gactgagccc gataccgcga tgtcctccag cgcgaatgcg
     2221 ccgctgtatg gcgccctgcg ccaggggccg gccgaatggg gcctgctgct ggcgctggtg
     2281 gccttgtggg gcagctcctt cctgctggtg cgcgtctcgc tcgacgcctt cacgccggtg
     2341 gcggtcaccg ctgggcggct gctgatcggc gcgggcgtgc tggtcctgct gctggtgttc
     2401 agtggacgtc gtccgccggg cggcgtgcgc acctggctgt tcttcacggc catggcgatc
     2461 atcggcaatg cgctgccgtt ctttctcatc gcctggggcc agcgcgacat accgtccgga
     2521 ctgactggca tcctgatggc cgtgatgccg ctggtggtgc tggtactggc gcatttcctg
     2581 gtggtgggcg agcggttgac gccgcgccgc ctggccggtt tcacgctggg ctttctcggc
     2641 gtcgtcgtgc tgaccggccc cgaggcacgc gccgccctgg tcggccgcga ggcgtccttc
     2701 atcgcccagc tcgccgtgct gggcgccgcc gtctgctacg gcatcgcggc catcatcgcg
     2761 cgccgcggcc cggtgctgaa gccggtggtc acggccgccg ccgtgttgtc catcgccggt
     2821 atcgtggtcg gcgcggtcct gctcgccagg ggcctgcgcg agggcgaggc ggcggtcgcg
     2881 cacgcgacca tcgcgaccgg gccggcgctg gccctgctgg cgctcggcgc cttgtcgacc
     2941 gggcttgcga ccgtcgtgta cttcagcctg gtggcgcgcg ccgggccgac cttcctgtcg
     3001 ctgatcaact acctgattcc cgtgtgggcc gtggcgctcg gggcgatggc gctcggcgaa
     3061 cggttgccga cgcgcgcatt tgcggccttg gcgctgattc tggccggcgt cgtgctggcg
     3121 cgctggcagt ttcgcggccg gcggttgccc gcatgaagat cgtcgtggtg ggcggcggct
     3181 acgcgggact ggcctgcctg gcgcgcctgg cacgcgtcct gcctgacgcc gagcgccacc
     3241 tggtggaacc gaaccgctgg cacctgcggc gcacgctgtt gcacgaaacc ctgcgccggc
     3301 cgctggccga cctgcgcgcc gatctcgccc cggtcgcgcg ccgctgggga ttccgccatc
     3361 atcgggcgcg catcgcgtgg acggcggagc ggctggtgga gtggtcgcgc gcgggccggc
     3421 tgccggtcgg gcgccgcgag ctggagttcg acgcgctggt cgtcaccacc ggcctgccgg
     3481 gcctggccgg ccgggtgtcg cgcagccgca cgccgccgat tccgctggag gcgctggcca
     3541 ccgggcccgg cgtcaatgcg tggaagcgac tcgccgcttc cgcagaccgc gtctgggtcg
     3601 tgggcgccgg cgccagcgga ctgcagtgcc tgttcgagct tgccgccgcg cgcccgaggg
     3661 gggcgccgct ggggctggtc gaggcgtccg acacgatcct cgcgtccgag cccgacgggc
     3721 tgcgccgcga agtgcttgaa cggctggatg cgctgcatct cgacctccgg ctcggcaccc
     3781 ggtacgacgg tttcgggcgc ggtcgggtgc gcatgcaggg tcctgccggc gcgctctccg
     3841 agccggccgc cggcgtcctg ctgtgcaccg ggccgaccgt gcaacgcatc gacgcgaatg
     3901 ccgccggcca ggtgcttcac cggcgccggc cgttgccgcg cgtcttcgcc gccggcgact
     3961 gtgcccgctg gcacggcgtg ccgttcgacg ccgccaccgc gcagaccgcc gtgcgcaagg
     4021 gcaggcaagt tgccgagacc gtcgcgcggg tggcggcggg ccgtgcgccc gacgactggg
     4081 atgcgcggca actcggcttc ttcctgagcc tcggtccgca tgacgccatc ggctgggtgt
     4141 tcgcgcgcgc cgcgctggtc agcggcctgc cggccgtcgc ggcgcgcgag gccgtcgaag
     4201 ctcgctggga cctgctgttg cgcggcatcg acagcttcgc cgccttctga aaaccggggt
     4261 cagacacctg tttccgctcg tctgcaggat cgtgccgcca tgggcgcgca tcgctcggcg
     4321 gatgtccgcg gcaccgggcc gccatgggcg cgcttcgttc ggcgaagtct gagctcactc
     4381 agcgcgccca tgacaccccg gtcccgctgc cgcgcagaca gctctgcctt cgtggctcgg
     4441 ctcggcagcc gtcgccgcca gcgttgtaat cccggcagcg ggcgtggggt gtcataggcc
     4501 ggccgagcga gtttttcc
//
LOCUS       DAOMPG010000199         4501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8060_length_4501_cov_4.775079, whole genome shotgun sequence.
ACCESSION   DAOMPG010000199 DAOMPG010000000
VERSION     DAOMPG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_4501_cov_4.775079"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1913)
                     /gene="groL"
                     /locus_tag="PLS34_10150"
     CDS             complement(288..1913)
                     /gene="groL"
                     /locus_tag="PLS34_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279198.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRP87864.1"
                     /translation="MAAKEVKFSDEARARMFRGVNVLANAVKVTLGPKGRNAVLEKSF
                     GSPTVTKDGVSVAKEIELTDKFENMGAQMVREVASQTSDEAGDGTTTATVLAQAILRE
                     GLKSVAAGMNPMDLKRGIDKASAAIVAELKKLSKPCEDRKAIAQVGTISANGDSEIGD
                     RIAEAMDKVGKEGVITVEDGSGLESELDVVEGMQFDRGYLSPYFINEQQTQSAVLENP
                     YILLHDKKVSNIREMLPLLEAVAKSGRPLLIVAEDVEGEALATLVVNTIRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLQLEKASLADLGEAKKVQVTKENTTIID
                     GHGKAEDIKGRIEQIRAQIEDATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALVRAVAGVAKLKGDNHDQDMGVQILLRAAEEP
                     LRQIVANAGEDGSVVLNKVREGDGNYGYNAATGEYGDLIKMGILDPTKVTRLALQNAS
                     SVSGLLLTTEVMVAELPKKEEKAPGMPDMGDMM"
     gene            complement(1966..2256)
                     /gene="groES"
                     /locus_tag="PLS34_10155"
     CDS             complement(1966..2256)
                     /gene="groES"
                     /locus_tag="PLS34_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRP87865.1"
                     /translation="MKIRPLHDRVVIKRLEEERTSPGGIVIPDTATEKPIKGEVIAIG
                     NGKILESGEVRPLDLKVGDRVLFGKYSGTEVKVDGQDVLVMREDDVMAVLEG"
     gene            2458..2796
                     /gene="cutA"
                     /locus_tag="PLS34_10160"
     CDS             2458..2796
                     /gene="cutA"
                     /locus_tag="PLS34_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRP87866.1"
                     /translation="MPDNECLVVLCTCPDGAPAASLAESLVDERLAACVNRLPGVASC
                     FRWEGRVQHETETLLLIKTTRARLAALTTRIRELHPYELPEVIAVPVMGGLEQYLDWV
                     RAGVTGLSTP"
     gene            2810..>4501
                     /gene="dsbD"
                     /locus_tag="PLS34_10165"
     CDS             2810..>4501
                     /gene="dsbD"
                     /locus_tag="PLS34_10165"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HRP87867.1"
                     /translation="MIRIFLLTLAALLPQLATAEPQLLRAEQAFRYTAELQDGELALR
                     WQVADGYYLYQQRFGFRSLDSAVTLGTPEMPAGIPYEDEFFGASIIHRGSFEIRVPVD
                     GVPAGRTLPIEIKSQGCADIGVCLPPQTWQADVPLLAAAVASATPGPQQAEPAARRAL
                     LSFLGGDRGAAQQEFLHPDQAFKTRATRRDGGVALVWDIAPGYYLYREQFAVSAEGAP
                     LPAEFPRGRIISDEFFGDVEVLYDSAETWVQPPTGAQTLQVRHQGCAEGGICYPPTTW
                     TVTLASLEAAPAGPARTAPAPAGGAPTQAPVSESDRLAALITGGALPAVLAVFFGIGL
                     LLAFTPCVLPMVPILSGLIVGQGPQVTTRRAFTLSAVFVLAMALTYTIAGVVVAMLGH
                     NLQATFQHPAVLIGFSIVFVALALAMFGFYELQLPSGLQTRMTALSNRQQSGTWLGAG
                     AMGVFSALIVGPCVAAPLAAALIVIGTAGDPVRGGAALFALSLGMGAPLLAFGASAGK
                     LLPRAGAWMATIKNLFGLLLLGVAVWMLERIVPPAGALALWAGLFFLAAVFLGALE"
BASE COUNT          600 a         1575 c         1520 g          806 t
ORIGIN      
        1 cccacaccac cggcgcggag cctgcaaacg cctcgtcggc gcacgcggcg gcggcggcgg
       61 gtgccgccgc ggccaggcgg cgccagcgct ccggcaggtc gcggcgcagc gccggtggca
      121 gggcatcgag cccggcggcg acaagatctc caggggccag cttgctcatc ggcggcaagc
      181 atatcagccg ccggaaaaaa cgaagccccg ctcggggcgg ggcttcgcgc ccggcaggcg
      241 ccgggccatc ggaggggatt ctcccccccc ggcgcaggat gcaatgccta catcatgtcg
      301 cccatgtcgg gcatgccggg ggccttctct tccttcttcg gcagctcggc caccatcacc
      361 tcggtggtca gcagcaggcc ggagaccgag gaggcattct gcagcgccag gcgcgtgacc
      421 ttggtcgggt caaggatgcc catcttgatc aggtcgccgt actcgccggt cgcggcgttg
      481 tagccgtagt tgccgtcgcc ctcgcgcacc ttgttgagca ccaccgaacc gtcctcgccg
      541 gcattggcga cgatctggcg cagcggctcc tcggcagcac gcagcaggat ctggacgccc
      601 atgtcctggt cgtggttgtc gcccttgagc ttcgcgaccc cggccacggc acgcaccagc
      661 gcgacgccgc ctcccggcac cacgccttcc tcgaccgccg cacgcgtggc atgcagcgca
      721 tcctcgacgc gcgccttctt ctccttcatc tcgacctcgg tggccgcacc gaccttgatc
      781 acggcgacgc cgccggcgag cttggcgacc cgctcctgga gcttctcctt gtcgtagtcg
      841 gaggtggcgt cctcgatctg ggcacggatc tgctcgatgc gacccttgat gtcctcggcc
      901 ttgccgtggc cgtcgatgat ggtggtgttt tccttggtga cctggacctt cttggcttca
      961 cccaggtcgg ccagcgaggc cttctcgagc tgcaggccga cttcctcgga gatcaccgtg
     1021 ccaccggtca ggatggcaat gtcctgcagc atggccttgc ggcggtcgcc gaagcccggg
     1081 gccttgacgg cggcgacctt gacgatgccg cggatggtgt tgaccaccag cgtcgccagc
     1141 gcctcgccct cgacgtcctc ggccacgatc agcagcggcc ggcccgactt ggccacggcc
     1201 tcgagcagcg gcagcatctc gcggatgttc gagaccttct tgtcgtgcaa caggatgtac
     1261 gggttctcga gcacggcgga ctgcgtctgc tgctcgttga tgaagtacgg cgagaggtag
     1321 ccgcggtcga actgcatgcc ctcgacgacg tccagttcgc tctccaggcc cgagccgtcc
     1381 tcaacggtaa tcacgccttc cttgccgacc ttgtccatcg cctcggcgat gcgatcgccg
     1441 atttcgctgt cgccgttggc ggagatggtg ccgacctggg cgatggcctt gcggtcctcg
     1501 cagggcttcg acaacttctt cagctcggcg acgatggccg cggaggcctt gtcgatgccg
     1561 cgcttcagat ccatcgggtt catgccggcg gcgaccgact tcaggccctc gcggaggatg
     1621 gcctgcgcca gcacggtggc ggtggtggtg ccgtcaccgg cctcgtcgga ggtctgcgag
     1681 gcgacttcgc gcaccatctg ggcgcccatg ttctcgaatt tgtcggtcag ctcgatttcc
     1741 ttggcgaccg atacgccgtc cttggtgacg gtgggggaac cgaagctctt ctcgagcacg
     1801 gcgttgcggc ccttcgggcc gagggtgacc ttgacggcgt tggcgaggac gttcacgccg
     1861 cggaacatcc gcgcgcgcgc ctcgtcactg aacttgactt cttttgcagc catggatggt
     1921 tgctcctggt aactgggttg gttgggacgg gtggcgaggg gcggatcagc cctcgagaac
     1981 ggccatcacg tcgtcttcgc gcatcacgag gacgtcctgg ccgtcgacct tcacctcggt
     2041 cccgctgtac ttgccaaaga ggacgcggtc gcccaccttc aggtcgaggg gccgcacttc
     2101 accgctctcc aggatcttgc cgttgccgat ggcaatgacc tcgcccttga tcggcttttc
     2161 ggtggcggtg tccgggatga cgatgccgcc gggtgacgtg cgctcttcct ccagacgctt
     2221 gatgacgacg cggtcatgaa gcggacgaat cttcattgtt gctttctcct tgaaagtcat
     2281 tgggttgcgc tgagcaaccc ggaccagacc attgggacga acttgttagc actcgccccg
     2341 agtgagtgct aatgataggg acggattcgc cggagtcaag ggcggcggaa gcgcgtggcg
     2401 cgttcttgcc gctgccgcta tccttgacgc ccccgatcgc cgctcgaagg gacgcaagtg
     2461 ccggacaatg aatgcctcgt cgtgctttgc acctgcccgg acggcgcgcc ggcggccagc
     2521 ctggcagagt ccctggtcga cgagcgtctg gcggcctgcg tgaaccgcct gcccggggtg
     2581 gcatcctgct ttcgatggga gggccgggtg caacacgaga ccgagacgct gctgctgatc
     2641 aagaccacgc gcgcgcgcct cgcggcgctc accacacgga tccgggaact gcatccctac
     2701 gaacttccgg aagtgatcgc ggtccctgtc atgggcggcc tggaacagta tctcgactgg
     2761 gttcgcgccg gcgtgaccgg cctatccact ccctgaaggg cgggcgacca tgatccgaat
     2821 cttcctgctg actcttgcgg cgctgctgcc gcagctggcg accgccgagc cgcagctgct
     2881 gcgcgccgag caggccttcc gctatacggc cgagctgcaa gacggcgaac tggcgctgcg
     2941 ctggcaggtc gcggacggct attaccttta tcagcagcgg ttcggcttcc gcagcctgga
     3001 ctccgcagtg acgctcggca cgcccgagat gcccgccggg atcccctacg aggacgagtt
     3061 cttcggcgcc agcatcatcc atcgcggcag tttcgagatc cgcgtacccg tcgacggcgt
     3121 cccggccggc cgcacgctgc ccatcgaaat caagtcgcag ggctgcgcgg acataggcgt
     3181 ctgcctgccg ccgcagacct ggcaggccga cgtgccgctg ctggcagccg ccgtggcgag
     3241 cgccacgccg gggccacagc aggcagagcc cgccgcgcgc cgcgcgctgc tgtccttcct
     3301 ggggggcgac cggggcgcgg cacagcagga atttctccac cccgaccagg cattcaagac
     3361 gcgcgccacg cgccgcgacg ggggcgtcgc gctggtgtgg gacatcgcac ccggctacta
     3421 cctgtaccgc gagcagtttg ccgtctcggc agagggcgcg cccctgccgg cggaattccc
     3481 gcgcggccgg atcatctcgg acgagttctt cggcgacgtc gaggtgctct acgactccgc
     3541 cgagacctgg gtgcagccgc cgaccggcgc ccagaccctg caggtgcgtc accagggctg
     3601 cgccgagggc gggatctgtt atccgccgac cacctggacg gtgacgctgg cctcactcga
     3661 ggccgcgcct gccgggccgg cacggaccgc gcccgccccc gccggcggcg cgccgacgca
     3721 ggccccggtt tccgagtccg atcgcctcgc cgccctgatc accggcggcg ccctgccggc
     3781 cgtgctggcg gtgttcttcg gcatcgggct gctgctggcg ttcacgccct gcgtgctgcc
     3841 gatggtgccg atcctctccg gcctgatcgt cggccagggc ccgcaggtca ccacgcggcg
     3901 agccttcacg ctgtcggccg tgttcgtgct ggccatggcc ctgacctaca ccatcgccgg
     3961 cgtggtggtg gcgatgctcg gtcacaacct gcaggcgacc ttccagcatc ccgccgtgct
     4021 gatcggcttc tcgatcgtgt tcgtggcgct ggcgctggcg atgttcggct tctacgaact
     4081 gcagctgccc agcggcctgc agaccaggat gaccgcgctg tccaaccgcc agcagtccgg
     4141 cacctggctg ggcgcgggcg ccatgggcgt gttttcggcg ttgatcgtgg ggccctgcgt
     4201 ggcggcgccg ctggccgcgg cactgatcgt catcggcact gccggcgacc cggtgcgcgg
     4261 cggcgcggcc ctgttcgccc tgtcgctggg catgggcgca ccgctgctgg cgttcggcgc
     4321 ctcggccggc aagctgttgc ccagggccgg ggcctggatg gcgaccatca agaacctgtt
     4381 cggcctgctg ctgctcggcg tggcggtctg gatgctggag cgcatcgtgc cgccggccgg
     4441 ggcgctggcg ctgtgggcgg gcctgttctt cctcgcggcg gtgttcctcg gcgccctcga
     4501 g
//
LOCUS       DAOMPG010000200         4491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8081_length_4491_cov_4.002029, whole genome shotgun sequence.
ACCESSION   DAOMPG010000200 DAOMPG010000000
VERSION     DAOMPG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_4491_cov_4.002029"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..647
                     /locus_tag="PLS34_10170"
     CDS             12..647
                     /locus_tag="PLS34_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRP87868.1"
                     /translation="MLRTDVAGMPLEWVDYRCAVRLYHTEQVAYECGRPLFRLRGGVN
                     ACTGLRTVVEVSSIIATRGHGGGYPSRDAYTPPLNNHTLFRRDANLCLYCGEHFLAVD
                     LSRDHVTPLSRGGRDLWTNVVTACRRCNNHKAGRTPEQAGMQLLAVPFQPTYAEYIYL
                     KGRRVLADQMEYLLAHFPRQSPLHQRLASTRAERRDAGPGHAGIAIFLAGA"
     gene            640..1512
                     /locus_tag="PLS34_10175"
     CDS             640..1512
                     /locus_tag="PLS34_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRP87869.1"
                     /translation="MPDLPAACAALAELGIRVDPATAKGVGGGCIHGAWRLAGPDGPV
                     FLKTCHATSAWLLEAEADGLAGLRQAAFLRVPAVLGHGTVGDTAWLALEWLELCPATP
                     ASERRLGEALARQHLAGGEHFGWRRDNAIGATPQPNEISDDWAAFFRAQRIGFQLELG
                     ARRGLPHRLLARGERLLAGIPALLADRRPAPALLHGDLWSGNRAADAGGAPVVFDPAV
                     HYGDPECDLAMARLFGGFDHEFFAAYHAQLPAAPGSARRERLYQLYHVLNHANLFGGG
                     YVGQAERLIDTLLE"
     gene            complement(1556..2227)
                     /locus_tag="PLS34_10180"
     CDS             complement(1556..2227)
                     /locus_tag="PLS34_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87870.1"
                     /translation="MPGNFLELSVWARDVAASVAFWEPLGFTHGAVGDTWKHRYAVLT
                     DGRLVLGLHEYEFDSPSLTWVRPDLVAALPALQAMGIEFDFAKTGADEFHEAGFRDPA
                     GQVVTLLEARTWSSVFDGTPSSSLLGYFLEYRYPARDPDATAAFWERLGLVRDADRHG
                     PRAVTGGINLAPRAALRTPQLVFAHQDLAGAAQALAERGFAARPEPDGSLLLDAPDGL
                     VIRLE"
     gene            complement(2227..3357)
                     /gene="hemH"
                     /locus_tag="PLS34_10185"
     CDS             complement(2227..3357)
                     /gene="hemH"
                     /locus_tag="PLS34_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRP87871.1"
                     /translation="MSRYIGSEGFQHGTRERLGVLVTNLGTPDAPETGALRRYLKEFL
                     WDPRVVEAPRPLWWLILNGIILRTRPSRSAAAYRAVWTEEGSPLLQIAQAQTDGIRER
                     LAARLSGPVEVVLGMRYGNPSIPAALRHLHDRNCRRLLVLPLYPQYSASTTASTFDAV
                     AAALMRWRWIPELRFITRYHDEDGYLAALAASIREHWAEHGRGEHLVMSFHGVPRRYL
                     DQGDPYHCQCVKTGRLLAERLELEDGSWSVTFQSRFGKEEWLKPYTDASIRALGARGL
                     KRIDVICPGFSADCLETIEEIGVENAGYFKEAGGEVLSYIPCLNTRPDHLDYLAELAA
                     RHLQGWPETGIAPDPALLAGQSQAARVRAERLIAAREAGELG"
     gene            complement(3429..>4491)
                     /locus_tag="PLS34_10190"
     CDS             complement(3429..>4491)
                     /locus_tag="PLS34_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRP87872.1"
                     /translation="YILAGSYVLILHAGDLPGVFALIFRSAFGPTEAAGAFVGGTLGY
                     AFLYGMKRALFSNEAGQGSSPIAHSAAKTKEPVREGIVAALEPFIDTIIVCTFTALVI
                     LSTGVWNRGPDTEYGVPPDIEEVAPGLWTLESGPAPIPPASDAAWFDGESVFMLVHAH
                     HSARSDTDLHKIYGTISGTGQGRPMIEWRSFASPVEPRLASRGLYVDYQGATLTARAF
                     DSVTPGLGKWLVTLAAWLFALSTMISWSYYGEQGMVYLAGERSVLPYKFVFCLLIIVA
                     TIGLVQTDRELDNLTGIGTGVMLFANLPIIWLFSRQAMAAYRDYVQRLDSGQVGPGHE
                     PPSIEDLVSGRDVAPRRRP"
BASE COUNT          669 a         1582 c         1596 g          644 t
ORIGIN      
        1 tgagccaaca gatcctgcgc accgacgtcg ccggcatgcc gcttgagtgg gtggattacc
       61 ggtgcgccgt gcgcctgtac catacggagc aggtcgccta cgaatgcggg cggccgctgt
      121 tccggctgcg cggcggcgtc aacgcctgca ccggtctacg cacggtggtc gaggtgtcga
      181 gcattatcgc cacccgcggc cacggcgggg gctacccctc gcgggacgcc tacacgccgc
      241 cgctgaacaa ccacacgctg ttccgccgcg acgccaacct ctgcctctat tgcggcgaac
      301 acttccttgc ggtcgatctg tcgcgcgacc acgtcacgcc cctgtcccgc ggcggccggg
      361 acctctggac caacgtggtc accgcctgcc ggcggtgcaa caaccacaag gccggccgga
      421 cgcccgagca ggccggcatg cagctgctcg ccgtgccgtt ccagccgacc tacgcggagt
      481 acatttacct gaaggggcgg cgcgtgctgg ccgaccagat ggagtacctg ctggcgcact
      541 tcccgcgcca gagcccgctg caccagcggc tggcctcgac gcgcgccgag cgccgggacg
      601 ccgggccggg ccacgccggg atcgccatat tcctcgccgg tgcctgatct gccggccgcc
      661 tgcgcggcgc tcgccgagct gggcattcgc gtcgatccgg ccacggcgaa gggcgtcggc
      721 ggcggctgca tacacggcgc gtggcggctg gccggcccgg acggcccggt cttcctgaag
      781 acctgtcacg cgacgtcggc ctggctgctg gaggccgagg cggacggcct ggccgggctg
      841 cggcaagccg ctttcctgcg cgtgccggcc gtgctcggtc acggcaccgt cggggatacc
      901 gcctggctgg cgctggagtg gctggagcta tgcccggcga cgccggcgtc ggagcggcgc
      961 cttggcgagg cgctcgcccg ccagcacctg gcgggcggcg agcacttcgg ctggaggcgg
     1021 gacaatgcca tcggcgcgac cccgcagccg aacgagatca gcgatgactg ggcggcgttc
     1081 tttcgggcgc agcgcatcgg cttccagctc gagctggggg cacggcgcgg actgccccac
     1141 cggctgctgg cccgcggcga gcgcctgctc gccgggatcc ccgcgctgct ggccgaccgc
     1201 cggcccgcgc cggcgctgct gcacggcgac ctgtggagcg gcaatcgggc cgccgacgcc
     1261 gggggcgccc cggtggtgtt tgacccggcg gtgcactacg gcgatcccga gtgcgacctg
     1321 gccatggcgc ggctgttcgg cggcttcgac catgaattct tcgccgccta ccacgcgcag
     1381 ctgccggcgg cgccgggcag cgcacggcgt gagcgcctgt accagctcta ccacgtgctg
     1441 aaccacgcca acctgttcgg cggcggctac gtcggccagg ccgagcggct gatcgacacc
     1501 ctgctggagt aatggggaca gtccccaatt ccagcggaat tggggactgt ccccattact
     1561 ccagccggat gaccaggccg tcgggcgcgt cgaggagcaa tgagccgtcc ggctccggcc
     1621 gggcggcgaa gccgcgctcc gccagtgcct gcgctgcgcc ggcgaggtcc tggtgcgcga
     1681 agaccagctg cggcgtgcgc aacgcggcgc gtggtgcgag gttgatgccg ccggtcacgg
     1741 cgcgcggccc atgacggtcg gcgtctcgca ccaggccgag acgctcccag aaggcggcgg
     1801 tggcgtccgg gtccctggcc gggtaacggt actcgaggaa atagcccagc aggctgctcg
     1861 agggcgtgcc gtcgaaaacg gacgaccagg tgcgggcctc cagcaaggtg accacctggc
     1921 ctgccgggtc gcggaacccc gcttcatgga attcatcggc cccggtcttg gcgaagtcga
     1981 attcgatacc catggcctgc agcgcgggga gcgccgcgac gagatccggc cgtacccagg
     2041 tcagcgacgg cgagtcgaac tcgtattcgt gcaaccccag caccaggcgc ccgtcggtga
     2101 gcacggcata gcgatgtttc caggtgtccc ccaccgcgcc atgcgtgaac ccgagcggct
     2161 cccagaaggc gaccgaggcg gcgacgtcgc gcgcccagac gctcagctcg aggaagttgc
     2221 ccggcatcag cccagctcgc ccgcctcccg ggcggcgatc agtcgctcgg cacgcaccct
     2281 ggcggcctgt gattgcccgg cgagcagggc cgggtccggt gcgatgccgg tctccggcca
     2341 gccctgcagg tgcctggcgg cgagctcggc caggtaatcg agatggtccg gtcgggtgtt
     2401 gaggcagggg atgtagctca gcacctcgcc gccggcctcc ttgaaatagc cggcgttctc
     2461 cacgccgatc tcctcgatgg tctccaggca gtcggccgag aacccgggac agatgacgtc
     2521 gatgcgcttg aggccgcgtg caccgagcgc acggatggag gcgtcggtgt agggcttgag
     2581 ccattcctcc ttgccgaagc gcgactggaa cgtgaccgac cagctgccgt cctccagctc
     2641 gagccgctcg gccagcaggc gcccggtctt cacgcactgg caatggtagg ggtcgccttg
     2701 gtcgaggtag cggcgcggca cgccgtgaaa gctcatcacc aggtgttcgc cgcggccgtg
     2761 ctccgcccag tgctcgcgga tactggccgc cagtgcggcg agatagccgt cttcgtcgtg
     2821 atagcgcgtg atgaagcgca gctcaggtat ccaccgccag cgcatcaggg cggccgccac
     2881 cgcgtcgaag gtcgacgccg tggtgctggc ggaatactgc gggtaaagcg gcagcaccag
     2941 caggcgccgg caattgcggt cgtgcaggtg ccgcagtgcc gccgggatcg agggattgcc
     3001 gtagcgcatg ccgagcacga cctcgaccgg cccggacagg cgcgctgcga gccgctcacg
     3061 aatgccgtcg gtctgcgcct gcgcgatctg cagcagcggc gagccttctt cggtccagac
     3121 ggcgcggtac gctgccgcgg aacgactcgg gcgagtccgc aggatgatgc cgttgaggat
     3181 cagccaccac agcgggcgcg gcgcctcgac cacccgcggg tcccaaagga attccttgag
     3241 gtagcgccgc agcgccccgg tttccggcgc gtcgggtgtg cccaggttcg tcaccaggac
     3301 gccgagtcgc tcgcgggtgc cgtgctggaa cccctcgctg ccgatgtatc tgctcatccg
     3361 aaatcctcca tgcaggcgcg cattatccta tgcgggcgcg ccgcgcgacc acgcggacga
     3421 cagcgcgctc acggccggcg gcgcggcgcc acgtcccgcc cgctcaccag gtcctcgatc
     3481 gacggcggct cgtgccccgg cccgacctgg ccggagtcca gtcgctgcac gtaatcccgg
     3541 taggccgcca tggcctggcg cgagaacagc cagatgatcg gcaggttggc gaacagcatc
     3601 acgccggtgc cgatgccggt caggttgtcg agttcgcggt ccgtctgcac cagcccgatg
     3661 gtggcgacga tgatcagcag gcagaagacg aacttgtagg gcagcaccga gcgctcgccc
     3721 gcgagataga ccatgccctg ctcgccgtaa tagctccacg agatcatggt tgagagcgcg
     3781 aacagccagg ccgccagcgt caccagccac ttgccgaggc ccggcgtgac cgagtcgaag
     3841 gcgcgcgcgg tgagcgtcgc accctggtag tcgacataca ggccgcgaga cgcgaggcgc
     3901 ggctccaccg ggctcgcgaa gctgcgccac tcgatcatcg gccggccctg gccagtgccg
     3961 ctgatggtgc cgtagatctt gtggaggtcg gtatcgctgc gcgcgctgtg gtgggcgtgc
     4021 accagcatga acaccgactc gccgtcgaac cacgcggcat cgctggccgg cgggatcggc
     4081 gccgggccgc tctccagcgt ccagagcccc ggggccacct cctcgatgtc cggcggcacg
     4141 ccgtactcgg tgtccgggcc gcggttccac accccggtgg acaggatcac cagcgcggtg
     4201 aaggtgcaca cgatgatggt gtcgatgaac ggctccagcg cggcgacgat gccctcgcgc
     4261 accggctcct tcgtcttggc ggcggagtgg gcgatgggcg aggagccctg gccggcctcg
     4321 ttggagaaca gcgcccgctt catgccgtac aggaaggcgt agcccagtgt gccgccgacg
     4381 aaggcgccgg ccgcctcggt cgggccgaag gccgacctga agatcagcgc gaagacgccg
     4441 ggcagatcgc cggcatgcag gatcagcacg tagctgcccg ccaggatgta c
//
LOCUS       DAOMPG010000201         4458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8158_length_4458_cov_4.113559, whole genome shotgun sequence.
ACCESSION   DAOMPG010000201 DAOMPG010000000
VERSION     DAOMPG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_4458_cov_4.113559"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..562
                     /locus_tag="PLS34_10195"
     CDS             224..562
                     /locus_tag="PLS34_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HRP87873.1"
                     /translation="MPGKRIFKVLFVNQGKVYEVYARNVLQGELFGFLAIEELVFGER
                     SSVVVDPAEERIRSEFAGVRRSYIPLHAVLRVDEVEKQGVSKISAVEGGNVAQFPMPV
                     YAPGDVPKGK"
     gene            572..1330
                     /locus_tag="PLS34_10200"
     CDS             572..1330
                     /locus_tag="PLS34_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP87874.1"
                     /translation="MRFAWLGSGSRGNAALVEADGCCVMIDCGFGLREVETRLARLGR
                     APADIDAILVTHEHSDHIGGVARFAARHEILVRASAGTTRGFRGELPPRLDRINPHEV
                     FAIGALEITPMPVPHDAREPCQFVLSDGASRLGVVTDLGHVTAHVIASLQGCDALAIE
                     ANHDGELLAAGPYPRPLKRRVGGELGHLSNAQAARLLAALDTTRLRHVVALHLSEVNN
                     TPALARAALAAPLGCRPEEIPVADQEAGLDWRTL"
     gene            1430..>4458
                     /gene="purL"
                     /locus_tag="PLS34_10205"
                     /gene_synonym="purI"
     CDS             1430..>4458
                     /gene="purL"
                     /locus_tag="PLS34_10205"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRP87875.1"
                     /translation="MLTLPGAPAFSRFRLDKFLEDLRGLCPVVTSVAARHMHFADLDR
                     ELDADELRLLKRLLSYGPSRTVAFPEGAFLLVVPRFGTISPWSSKATDIAHNCGLAAV
                     RRLERGVAWHIVSRRPLRDGELDALAGPLHDRMTESVVPRIDDAARLFERHAPAPLGF
                     VDALGGGRAALVAANGALGLALSDDEIDYLLAHYAGAGRNPSDAELMMFAQANSEHCR
                     HKIFNASWVVDGEPQPRSLFAMIRNTHEKSPGGVLSAYKDNASVIEGWDGERFFPSPA
                     DARYDYVSEPVHILMKVETHNHPTAISPFPGAATGAGGEIRDEGATGLGARPKAGLAG
                     FTVSHLRIPGLEQPWEAEGPGFPGRIATPLEIMRDGPIGAAAFNNEFGRPNLAGYFRT
                     YEQMVDGVVRGYHKPIMIAGGLGNIRAGHVEKGDVAPGAKLVVLGGPAMLIGLGGGAA
                     SSMASGESTEDLDFASVQRGNPEIQRRAQEVIDRCWAMGDDNPIMLIHDVGAGGLSNA
                     LPEVIDHSGLGGRIELREVPNDEPGMSPMAIWCNEAQERYVLAVAAARVEDFSALCVR
                     ERCPWAVVGETTAERRLLVSDRQFGNTPVDMPVEVLLGKPPRMTRSVERVARRGDALP
                     VVTDLREAALRVLQLPAVADKTFLVTIGDRSVGGFTARDQMVGPWQVPVADVAVTTTG
                     YRGYTGEAMAMGERTPLAVLDGPASGRMAVGEALTNIAAAAVPSLGHVRLSANWMAAC
                     GETGEDAALFDTVRAVGEALCPSLGIAIPVGKDSLSMRTRWESAHGERAVIAPLSLIV
                     SAFAPVEDARRTLTPQLRLDLGETVLVMIDLGACRNRLGGSALAQVYGRTGAEPPDLD
                     DPARLASFFTGIQALNAAGQLIAYHDRSDGGLLATLAEMAFAGHCGIDADIGALGDDP
                     VAALFAEELGAVVQVRANDLQAVLDTFAVAGLGDCTAVIGAPTESDRLRIRRGAQTLL
                     DEARRELRDAWSQTSYRMQALRDHPDSAREEHLARLD"
BASE COUNT          647 a         1557 c         1593 g          661 t
ORIGIN      
        1 ccgtgcacgc tgaacacgcg caggctgtca gcgccgagaa tgtgccgctg cttgctgacg
       61 atcttcaggg tcaggggcat gggctatccc ggaaaaacat tgacttgtat ggccactcgt
      121 caaaaatagt tgaactcgtg ccaagcgaca acagcgcata atgtgcgccg cgtcacgaat
      181 tcccccggta ccgcgccttt cgtcccatca agcagtgagt tccatgccag gcaaacgcat
      241 tttcaaggtc ctgttcgtca accagggcaa agtctacgaa gtctacgccc gcaacgttct
      301 gcagggcgaa ctgttcggct tcctggcgat cgaggaactc gtgttcggcg aacgcagttc
      361 ggtggtggtg gatccggcag aggagcgcat caggtcggaa ttcgccggtg ttcggcgcag
      421 ttacattccg ttgcacgcgg tgctgcgggt ggacgaggtg gaaaagcagg gcgtcagcaa
      481 gatcagcgcg gtggaaggcg gcaacgtggc ccagttcccg atgccggtgt acgcgcccgg
      541 cgacgtgccg aagggcaagt agggcgcgcc ggtgcgattc gcctggctcg gcagcggcag
      601 ccgtggcaat gccgcgctgg tggaggcgga cggctgctgc gtgatgatcg actgcggctt
      661 cgggctgcgg gaggtcgaga cgcggctggc gcggctcggc agggccccgg ccgacatcga
      721 cgccatcctg gtcacccatg agcacagcga ccacatcggc ggcgtcgcgc gtttcgccgc
      781 ccggcacgag attctcgtca gggcgagcgc cggcaccacg cgcggttttc gcggcgaact
      841 gccgccgcgg ctcgatcgca tcaaccccca cgaggtgttc gcgatcggcg cgctcgagat
      901 cacgccgatg ccggtgccgc acgacgcccg cgagccctgc cagttcgtgt tgagcgacgg
      961 cgcgtcgcgg ctcggcgtgg tcacggatct cggccacgtg accgcgcacg tcatcgccag
     1021 cctgcagggc tgcgatgcgc tggccatcga ggccaaccac gacggcgagc tgctggccgc
     1081 aggcccgtac ccgcgaccgc tcaagcgacg ggtgggcggc gaactcggcc acctttcgaa
     1141 cgcgcaggcg gcgaggctgc tggccgcgct cgacacgact cggctgcgtc acgtcgtcgc
     1201 cctgcacctg tcggaggtca acaacacgcc ggcgctggcc cgcgcggcgc tggccgcgcc
     1261 gctgggttgc cggccggagg agattcccgt cgccgaccag gaggccggcc tggactggcg
     1321 cacgctgtga ggcgccgacg gggcccggcc gcggcccgcg ggtgggcatc cggggctccc
     1381 ctgaagtaga attcgccggc cttccgcttc gcgcccgccc agagaaaaca tgctgaccct
     1441 gcccggtgcg ccggctttct cccgtttccg cctcgacaag ttcctggagg acctccgggg
     1501 cctgtgcccg gtcgtcacct cggtggccgc gcggcacatg catttcgcgg acctcgaccg
     1561 cgagctggac gccgacgaac tgcgcctgct gaagcggctg ctgagctacg gcccgagccg
     1621 caccgtggcc tttccggaag gggccttcct gctggtggtg ccgcgcttcg gcaccatctc
     1681 gccctggtcg agcaaggcca ccgacatcgc ccacaactgc gggctggcag cggtgcgccg
     1741 gctcgagcgc ggcgtggcct ggcacatcgt ttcccgccgc ccgctgcgcg acggcgagct
     1801 ggacgccctg gccggcccgc tgcacgatcg catgaccgag tcggtggtgc cgcggatcga
     1861 cgacgcggca cggctgttcg agcgtcacgc gcccgcgccg ctcggcttcg tggatgcgct
     1921 gggaggcggc cgcgcggcgc tcgtcgcggc caacggcgcg ctgggcctgg cgctgtcgga
     1981 tgacgagatc gattacctgc tggcgcatta cgcgggcgcc ggccgcaatc cgagcgatgc
     2041 ggagctgatg atgttcgccc aggccaacag cgagcattgc cgccacaaga tcttcaacgc
     2101 gagctgggtg gtggacggcg agccccagcc caggtcgctg ttcgcgatga tccgcaacac
     2161 ccacgaaaag tcgccgggcg gcgtgctgtc cgcctacaag gacaacgcgt ccgtgatcga
     2221 gggctgggac ggcgagcgct ttttcccgtc gccggccgat gcgcgctacg actacgtcag
     2281 cgagcccgtg cacattctca tgaaggtcga gacgcacaat cacccgaccg ccatttcgcc
     2341 ctttccgggc gccgccaccg gcgccggcgg cgagattcgc gacgagggcg ccaccggcct
     2401 gggtgccagg ccgaaggccg gcctggccgg cttcaccgtg tcccacctgc gcatccccgg
     2461 cctggagcag ccctgggaag cggaaggccc gggtttcccc ggccgcatcg ccacgccgct
     2521 ggagatcatg cgcgacggcc cgatcggcgc ggccgccttc aacaacgagt tcggccggcc
     2581 gaacctggcg ggctatttcc gcacctacga gcagatggtc gacggcgtgg tgcgcggcta
     2641 ccacaagccg atcatgatcg ccggcggcct gggcaacatc cgcgccgggc acgtcgagaa
     2701 gggcgatgtc gcgcccggtg cgaagctggt cgtgctcggc ggcccggcga tgctcatcgg
     2761 ccttggcggg ggcgccgcct cgtcgatggc gagcggcgag agcaccgagg atctcgattt
     2821 cgcgtcggtg cagcggggca atcccgagat ccagcgccgg gcgcaggaag tcatcgaccg
     2881 ctgctgggcg atgggcgacg acaacccgat catgctgatc cacgacgtcg gcgcgggcgg
     2941 cttgtcgaac gccctgcccg aagtcatcga tcacagcggg ctcggcggac gcatcgagct
     3001 gcgcgaggtg cccaacgacg agcccggcat gtcgccgatg gcgatctggt gcaacgaggc
     3061 ccaggaacgc tacgtgctgg cggtggccgc ggcacgcgtc gaggatttct ccgcgctgtg
     3121 cgtgcgcgag cgctgtccgt gggcggtggt cggcgaaacc acggccgagc ggcgcctgct
     3181 ggtcagcgac cgccagttcg gcaacacccc cgtggacatg ccggtcgagg tgctgctcgg
     3241 caagccgccg cgcatgaccc gcagcgtcga gcgcgtggcg cggcgcggcg acgcgctgcc
     3301 ggtggtgacc gacctgcgcg aggcggcgct gcgcgtgctg cagctgcccg cggtcgcgga
     3361 caagactttt ctcgtcacca tcggcgatcg cagcgtcggc ggcttcaccg cccgcgacca
     3421 gatggtcggg ccctggcagg tgccggtggc cgacgtcgcg gtcactacca cgggctatcg
     3481 gggctatacc ggcgaggcca tggccatggg tgagcgcacg ccgctggcgg tgctcgacgg
     3541 gccggcctcc ggccgcatgg cggtgggcga ggcgctcacc aacatcgccg ccgccgccgt
     3601 gccgtcgctg ggccacgtgc ggctgtcggc caactggatg gcagcctgcg gcgagaccgg
     3661 cgaggatgcc gcgctgttcg acacggtgcg cgcagtgggc gaagcgctct gtccgtcgct
     3721 gggcatcgcc atcccggtcg gcaaggactc gctgtccatg cgcacgcgct gggaatccgc
     3781 ccacggcgag cgcgccgtga tcgcaccgct ttcgctgatc gtctcggcgt tcgcgccggt
     3841 cgaggatgcg cggcgcacgc tcacgccgca gctgcgcctc gacctcggcg agaccgtgct
     3901 ggtgatgatc gatctcggcg cgtgccgcaa ccggctcggc gggtcggcgc tggcgcaggt
     3961 gtacggtcgc acgggcgccg aaccccccga cctcgacgac cccgcccgcc tggcgtcctt
     4021 tttcaccggc atccaggcgc tgaacgccgc cgggcaactg attgcctatc acgatcgctc
     4081 ggacggcggg ctgctggcga cgctcgccga gatggccttc gccggccatt gcggcatcga
     4141 cgccgacatc ggcgcgctcg gcgacgatcc ggtggcggcg ctgtttgccg aggaactcgg
     4201 cgccgtcgtg caggtgcgcg caaacgacct gcaggcggtg ctcgacactt tcgcggtcgc
     4261 cggcctcggc gactgcactg ccgtgatcgg cgcgcccacg gagagcgacc ggctgcgcat
     4321 ccggcgcggc gcgcagacgc tgctcgacga ggcgcgccgg gagctgcgcg acgcctggtc
     4381 gcagaccagc taccgcatgc aggcgctgcg cgatcacccg gacagcgcgc gcgaggaaca
     4441 tctcgcgcgc ctggacga
//
LOCUS       DAOMPG010000202         4418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8235_length_4418_cov_4.614944, whole genome shotgun sequence.
ACCESSION   DAOMPG010000202 DAOMPG010000000
VERSION     DAOMPG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_4418_cov_4.614944"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLS34_10210"
     CDS             complement(<1..272)
                     /locus_tag="PLS34_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87876.1"
                     /translation="MAELLPSLRLDRVTHQPGDDCALIPTGKGFVELVFHFVRHAEID
                     GGHGPASIVENFNNIMDSSMHVGKHSMRSGAEKTGVRHPFRSGIETG"
     gene            362..781
                     /locus_tag="PLS34_10215"
     CDS             362..781
                     /locus_tag="PLS34_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP87877.1"
                     /translation="MILVVDASVALKWFFRTADDETDAASALEILEGVASGGIELVQP
                     PHFIAEMAAVLVRKKLTGALEDLRDLQDVSMRTAGGPDLHLVAADIAMRTSAHVFDTL
                     YHAAALLNPPAELVTADGRYYRSARHLGNIRLLEETA"
     gene            916..>2132
                     /locus_tag="PLS34_10220"
     CDS             916..>2132
                     /locus_tag="PLS34_10220"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP87878.1"
                     /translation="MGVATKLGRKLGRKNKAHVTLIDCAESHIWKPLLHEVATGALDT
                     GIDAISYRGHAAANGYHFQQGRLKDIDRERREIVLEGIYEGGVELLPERRIGFDYLVV
                     AIGSIANDFRIPGVKRYCLFLDNTEQALAIRKILLNRFLRYASHEQLDEKIKVAIVGA
                     GATGTEMAAEMHHAVDQLRGYGYKIDSSLLDVTLIEADQRILPKVSQPEISHAVAREL
                     AKIGVEVLTNTRVVEVTDRGITFQGGYFIAADIVIWSAGIRGPDFLKDIAGLETNGLN
                     QLVVRPTGQTTRDDRIFAIGDCSGCPQEDGSWVPPRGQAARQLGHLVADNIMLMMQGR
                     PAEKTYVYRDLGSLVSLSRFHTVGNLFSFFRGGILVEGKIARFAYLSLYRRHLIEMYG
                     PVRGLLMVLLKSL"
     assembly_gap    2133..2163
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2293..2553
                     /locus_tag="PLS34_10225"
     CDS             2293..2553
                     /locus_tag="PLS34_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HRP87879.1"
                     /translation="MKTTLDLSDAVLERTKAYAARQGTTLRAVVEEALRRYLDGQDTG
                     ATRSVFRIQPWGRGGFLPGYEDRTWNEILDEVNQRSTPDDRG"
     gene            2540..3013
                     /locus_tag="PLS34_10230"
     CDS             2540..3013
                     /locus_tag="PLS34_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRP87880.1"
                     /translation="MIAVDTNILVYAHRADLQWHEAARSQVESLAAGPAPWGVPMHCL
                     HEFYATVTRRGPLAEPTPGRIAMAQLDALLACESLLVLTEDGEHWRVLMALIEAGGVN
                     GGAVHDARIAACCLRHGVAELWTADRDFSRYPALRTRNPLIAVNERRPRYRSRTR"
     gene            complement(3078..3299)
                     /locus_tag="PLS34_10235"
     CDS             complement(3078..3299)
                     /locus_tag="PLS34_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP87881.1"
                     /translation="MGKRKVRLTNCIRERRAGADGMTQQQLADLVGVTRQTINAIEAN
                     KYSPSLEVAFLIARALDARLEELFGIVEG"
     gene            complement(3303..3731)
                     /locus_tag="PLS34_10240"
     CDS             complement(3303..3731)
                     /locus_tag="PLS34_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87882.1"
                     /translation="MSMTFQEKSLWVTLAGLLVVSGGYAYSAYMTLLHMPAARDVMPQ
                     HAGLFMGATVMLVIILVAGHIVIALLDRRTDPDERDRLIALRGQRYGSFVLATGVFLA
                     LCTALVTEGNAIMAHVLLGSWVLAQVVEIVSQLVLYRRGS"
     gene            3921..4127
                     /locus_tag="PLS34_10245"
     CDS             3921..4127
                     /locus_tag="PLS34_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRP87883.1"
                     /translation="METVTISPKFQVVIPRRIREALNLKVGQKIRVLQYDQRVEFVPI
                     MAPGEARGFLAGLETDVPRDPDRQ"
     gene            4124..>4418
                     /locus_tag="PLS34_10250"
     CDS             4124..>4418
                     /locus_tag="PLS34_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP87884.1"
                     /translation="MNLVDSSGWLEYFADGPNAGFFAPAIEQGDALLVSSIDLYEVFK
                     QILQQRDEDSALKAIAVMGQGRVVPVDDRIALSAVGLSVRHQLPMADSLILASA"
BASE COUNT          778 a         1459 c         1425 g          725 t
ORIGIN      
        1 cccgtttcga tcccggatcg aaacgggtgt ctgaccccgg ttttttcagc gccagagcgc
       61 atgctgtgct tgccgacatg catcgagcta tccataatgt tgttgaagtt ctcaacaatc
      121 gaggcgggtc catggccacc gtcaatttca gcgtgccgga cgaagtgaaa gacgagttca
      181 acgaaacctt tgccggtcgg aataagagcg cagtcatcgc ccggctgatg cgtgacgcgg
      241 tcgaggcgga gcgaaggcag cagctcagcc atgcagccat ccgtcgcatt ctcgaccggc
      301 gcagcgctgc cccgcgcgtc gctgatgaca agctacgaca ggttaggcgt gtgcgacgtc
      361 catgattctc gtggtggacg cgagcgtcgc gctgaaatgg tttttcagga ccgctgacga
      421 cgagacggat gccgcgtcgg ccctggagat cctggagggc gttgcgtccg ggggcatcga
      481 gctcgtccag ccgccccact tcattgccga aatggctgcg gtgctggtgc gcaagaagct
      541 gacgggtgcg ctcgaagacc ttcgcgacct ccaggacgtc agcatgcgca ctgcaggcgg
      601 cccggacctg catctggtgg cggcagatat cgcgatgcgg accagcgcgc acgtgttcga
      661 cacgctctat cacgccgccg cgctgctcaa cccgccggcc gagctcgtaa cggcggacgg
      721 gcgctactac cggagtgcgc ggcacctcgg gaatatccgc ttgctggaag aaaccgcctg
      781 agctggcctc aagctcattt gcgtgaaaat cgtccccggc cgccccttga tgctgcactg
      841 cggtagaatg ggccgtcttt ctcgcgaggc cggcccgtgg aaaaaatcgt catcgtcggc
      901 ggcggggcag ggggcatggg cgtcgccacc aagctcggcc gcaagctggg ccgcaaaaac
      961 aaggcgcacg tgacgctgat cgactgcgcc gagagccaca tctggaagcc gctgctccac
     1021 gaggtggcca ccggcgcgct ggataccggc atcgacgcca tcagctaccg cggccatgcc
     1081 gccgccaacg gctaccactt ccagcagggt cggctgaagg acatcgaccg cgagcgccgc
     1141 gagatcgtcc tcgaaggcat ctacgagggc ggcgtcgagc tgttgccgga gcggcgcatc
     1201 ggctttgatt acctggtggt ggccatcggc agcatcgcga acgacttccg catccccggc
     1261 gtgaagcgct actgcctgtt cctcgacaac accgagcagg cgctggccat ccgcaagatc
     1321 ctgctgaacc gcttcctgcg ctacgccagc cacgagcagc tcgacgagaa gatcaaggtc
     1381 gccatcgtcg gtgccggcgc caccgggacc gagatggccg cggagatgca tcacgccgtg
     1441 gaccagctgc gcggctacgg ctacaagatc gactcctcgc tgctggacgt gacgctgatc
     1501 gaggccgacc agcgcatcct gccgaaggtc agccagccgg aaatctccca tgccgtggcc
     1561 cgcgagctgg cgaagatcgg cgtcgaggtg ctgaccaaca cccgcgtggt cgaggtcacc
     1621 gaccgcggca tcaccttcca gggcggctat ttcatcgccg ccgacatcgt catctggtcg
     1681 gccggcatcc gcgggccgga cttcctcaag gacatcgccg ggctggagac caacgggctg
     1741 aaccagctcg tggtccggcc gaccggccag acgacgcgcg acgaccgcat tttcgccatc
     1801 ggcgactgct cgggctgtcc ccaggaagac ggcagctggg tgccgccgcg cggccaggcg
     1861 gcgcgccagc tcggccacct ggtggcggac aacatcatgc tcatgatgca gggccgcccg
     1921 gcagagaaaa cctacgtcta ccgggatctc ggctcgctgg tcagcctgtc caggttccac
     1981 accgtcggca acctgttctc cttcttccgc ggcggcatcc tggtggaggg gaagatcgcg
     2041 cgcttcgcct acctgtcgct gtatcgccgc cacctgatcg agatgtacgg cccggtacgc
     2101 gggctgctga tggtgttgct caagagcctg aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnngaccgga aaacggaaaa acggggtcag acaccggttt cccgcaggaa accggtgtct
     2221 gaccccgttt tccggccagt tgtccggccg cagcgaatat gggagtatgg gcgtcattat
     2281 acgggcgacc atatgaagac gaccctggat ctcagcgacg cggtactgga acgaaccaag
     2341 gcgtacgccg cgcgccaggg caccacgctg cgcgcggtgg tcgaggaggc cctgcgccgc
     2401 tatctcgacg gccaggacac gggcgccacc cggagcgtgt tccgcatcca gccctggggc
     2461 cggggcggtt tcctgccggg gtacgaggac aggacctgga acgagatcct ggacgaggtc
     2521 aaccagcgat ccacgcctga tgatcgcggt tgacacgaac atcctcgttt acgcgcatcg
     2581 ggcggacctg cagtggcacg aggcggcgcg cagccaggtc gagtcgctcg cggccggtcc
     2641 ggcgccctgg ggtgtgccca tgcactgcct ccacgagttt tatgcgaccg tcacccgccg
     2701 cgggccgctg gcagagccca cgcccgggcg tatcgccatg gcacagctcg acgccctgct
     2761 ggcctgcgaa agcctgctgg tgctgacgga ggacggcgag cactggcgtg tgctcatggc
     2821 gctcatcgag gccggaggag tcaatggcgg cgcggtgcat gacgcccgca tcgccgcctg
     2881 ttgcctgcgt cacggtgtag cggagctgtg gaccgcggac cgcgacttct cgcgctaccc
     2941 tgcactccgc acgcgcaatc cgcttatcgc tgtcaacgag cggcgtccgc ggtaccggtc
     3001 acgcacgcgg tgaaagccga aaaacggggg cagacaccag tttccgcagg aaactggtgt
     3061 ctgaccccgt tttttagtca gccctcgacg atgccgaaga gttcctcgag ccgcgcgtcc
     3121 agcgcccggg cgatcaggaa ggccacctcg agcgagggcg agtacttgtt ggcctcgatc
     3181 gcgttgatcg tctggcgcgt tacgccgacc aggtccgcga gttgctgctg ggtcatgccg
     3241 tcggcgccgg cccggcgctc ccggatgcag ttcgtcaacc tcaccttgcg cttgcccacg
     3301 cctcagctcc cgcgccggta cagcaccagc tgcgagacga tctcaacgac ctgggccagc
     3361 acccaggacc ccagcagcac gtgcgccatg atcgcgttgc cctccgtcac cagcgcggtg
     3421 cacagcgcga gaaagacgcc ggtcgccagc acgaacgagc cgtagcgttg gccccgcaac
     3481 gcgatcaacc ggtcccgctc gtccgggtcc gtgcgccgat cgagcaaggc gatcacgata
     3541 tggccggcga ccaggatgat caccagcatg accgtcgccc ccatgaacag gccggcgtgc
     3601 tgtggcatca cgtccctggc cgcgggcata tggaggaggg tcatataggc ggagtaggcg
     3661 tagccgcccg agacgacgag cagccctgcg agcgtgaccc acaggctctt ttcctggaac
     3721 gtcatggaca tgaccggccc tcctgtgagc ctcatcgggg atgtaagaaa aacacgacat
     3781 gggcagtcta gtggcgccgg gaacacggtg tcaactattt cttacacctc tccgcggccg
     3841 gtccgtttcc acgggccgat ggttgcccga cggagccact ttcactagta tgacgagagc
     3901 tctatcgatc gcggatgcgt atggaaaccg tcaccatctc gccgaaattc caggtggtca
     3961 tcccgcggcg catccgcgag gcgctcaacc tgaaggtcgg ccagaagatt cgcgtgctcc
     4021 agtacgacca gcgcgtggag ttcgtcccga tcatggcgcc cggcgaggcg cgcgggttcc
     4081 tcgcgggact cgagactgac gtaccgcgcg acccggaccg ccagtgaacc tggtcgattc
     4141 ctccgggtgg ctggagtatt tcgcggacgg gccgaacgcc gggtttttcg cgccggccat
     4201 cgagcagggc gacgccttgc tcgtctcgtc catcgatctc tacgaggtct tcaagcagat
     4261 cctgcagcag cgcgacgagg acagcgcgct caaggccatt gcggtgatgg gccagggtcg
     4321 cgtcgtgccg gtcgacgacc gcatcgcact cagcgccgtc ggcttgtcgg tccggcatca
     4381 gctgccgatg gccgacagcc tgatccttgc atccgcgc
//
LOCUS       DAOMPG010000203         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8246_length_4412_cov_3.890291, whole genome shotgun sequence.
ACCESSION   DAOMPG010000203 DAOMPG010000000
VERSION     DAOMPG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8246_length_4412_cov_3.890291"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLS34_10255"
     CDS             <1..356
                     /locus_tag="PLS34_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRP87885.1"
                     /translation="HIVSNVRGELADGVGPGAAIRAVFPGGTITGCPKVRCMEIIQEL
                     EAGPRGAYTGSMGWLGADGSLDLNILIRTIEMSRSELRFRSGAGIVFDSVPEAELAET
                     RAKAKGLLLALEADS"
     gene            353..1168
                     /gene="pabC"
                     /locus_tag="PLS34_10260"
     CDS             353..1168
                     /gene="pabC"
                     /locus_tag="PLS34_10260"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HRP87886.1"
                     /translation="MILVDGMATGTVAATDRGLNYGDGLFETMRLHQGRVCLLARHLQ
                     RLKHGCERIALAYPGDALIEADLAALGTGATGEGVVRLVLTRGDGGRGYTPPPTAGVR
                     RIVSLHAVPELGTAGLSVGICRTRLGRSSALGGLKHLGRLEQVLAARETAAAGWDEGL
                     MLDDHDRVIEGTRHNLFFVRDGGVWTPRLAGCGVAGVMRELVLEALDKLVLGGGEAEL
                     RYHELHEVEELFLCNAVAGVRPVSHLAGRALAGAEVACALRAPLAAAGVAWLG"
     gene            1156..2175
                     /gene="mltG"
                     /locus_tag="PLS34_10265"
     CDS             1156..2175
                     /gene="mltG"
                     /locus_tag="PLS34_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRP87887.1"
                     /translation="MARLMVLLLGALLLAGGAAAWWGWQQLDTPGPGREDVVLEVPQG
                     ASLARIAARLEEAGVVRHGRLFEWYGRGSGLAGRLRAGEYRFPAGSTPREVMARLVSG
                     DVVLHSFTIVEGWTFRLMRTALEAHPAIRSTLEALPDEEVMARLGRPGEHPEGWFLPE
                     TYRFPRGTSDLEVLRLSHQAMQRALEAAWSRRRDALPFETPYEALILASIIEKETGLA
                     AERPEIAGVFVRRLERGMRLQTDPTVIYGAGPGFEGRLRSIHLDTDTPYNTYTRGGLP
                     PTPIALPGRASLEAAVQPLEGKSLYFVATGLPDGSHVFSETLAEHNRAVQRYWATVRE
                     RRNQR"
     gene            2172..2831
                     /gene="tmk"
                     /locus_tag="PLS34_10270"
     CDS             2172..2831
                     /gene="tmk"
                     /locus_tag="PLS34_10270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRP87888.1"
                     /translation="MNASATPATPGRFITLEGGEGVGKSTNLAFVAALLESAGLDVLQ
                     TREPGGTPLAERVREIIVSPAGEPVPPMAELLLIFAARALHIENLIRPALKAGRWVLC
                     DRFTDASYAYQGAARGLGEATVGWLEARVQGTLRPDLTLLLDVDPAVGLARAGARGAA
                     DRFEQERIDFFARVRAGYLARAAAEPDRFRVIDAAQPLDAVRRAITQHINEYVQANGK
                     T"
     gene            2818..3822
                     /locus_tag="PLS34_10275"
     CDS             2818..3822
                     /locus_tag="PLS34_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87889.1"
                     /translation="MEKLEFPWHAAAWNEFEAALAAERLGHAYLIEGPQGLGKLHLAF
                     RMAARVLGAPWPAADPPVPLQHPDLNWITLETDDEGKQKKQIGIHQVREACANLAMTS
                     YSGGWKVAVVWPAEQLNPHSANALLKTLEEPAPRTLLLLVRSRLDTLPATIASRCQRV
                     RIAPPPAAEAIAWLEARADGGDWRRLLSLAGDAPLLALLLAEEGFRELDGKFAADLVG
                     ILGRRREALEVAAEWARQPLPMTLRWLDVWTSELVRWKAAGAIPALESAVQRALQTAL
                     EGIPLQRLFRYRDEVRSAMTRNDGVFNTQMVLEGLLVPWADRLEPVRGDAALGMLSDR
                     "
     gene            3819..4163
                     /locus_tag="PLS34_10280"
     CDS             3819..4163
                     /locus_tag="PLS34_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP87890.1"
                     /translation="MTTSKPGLLTLTIKDKSALYLAYMPYVKNGGLFIPTSSNYRLGD
                     EVFMLLNLMGEDEKIPVAGRVIWVTPKGAQGKRTAGIGVQFSEQDRGSTQRRIETYLA
                     GALGGDKPTHTM"
     gene            complement(4219..>4412)
                     /locus_tag="PLS34_10285"
     CDS             complement(4219..>4412)
                     /locus_tag="PLS34_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRP87891.1"
                     /translation="FIRLKLKSLDKSTRYVVCCDTGRRSSAAAYILSERGFDAHVLKG
                     GLTMADPEGERRKSAESAD"
BASE COUNT          657 a         1501 c         1610 g          644 t
ORIGIN      
        1 accacatcgt ctccaacgtg cgcggcgagc tcgccgacgg cgtcgggccg ggcgcggcga
       61 tccgcgcggt gtttccgggc ggcaccatca ccggctgccc caaggtgcgc tgcatggaaa
      121 tcatccagga actcgaagcc ggcccgcgcg gcgcgtacac cggctccatg gggtggctcg
      181 gcgccgacgg ctcgctggac ctgaacattc tcatccgcac gatcgagatg agccgcagcg
      241 agctgcgttt ccgctccggc gccggcatcg tgttcgactc ggtgcccgag gccgagctcg
      301 cagagacccg cgccaaggcc aagggcctgt tgctggcgct ggaggcggac tcgtgatcct
      361 ggtcgacggc atggccaccg ggacggtcgc ggcaaccgac cgcggactga attacggcga
      421 cgggctgttc gagaccatgc ggctgcacca gggccgggtc tgcctgctgg cacggcacct
      481 gcaacggctc aagcacggct gcgagcgcat tgcgctggcc tatcccggcg atgcgctcat
      541 cgaggcggac ctggcggcgc tgggcacggg tgcaacgggc gagggcgtgg tgcgcctggt
      601 cttgacccgc ggcgacggcg ggcggggcta cacgccgccg cccaccgccg gggtccgccg
      661 catcgtctcg ctgcacgccg tgcccgagct cggcaccgcg ggactgtcgg tcggcatctg
      721 ccggacccgg ctcgggcgca gcagcgcgct gggcggcctg aagcacctgg gacggctgga
      781 gcaggtactg gctgcgcggg aaaccgccgc ggccggctgg gacgagggcc tgatgctcga
      841 cgaccacgac cgggtcatcg aaggcacgcg ccacaacctg ttcttcgtgc gcgacggcgg
      901 cgtatggacc ccgcggctgg ccggctgcgg cgtggccggc gtgatgcgcg aactcgtgct
      961 cgaggcgctc gacaagctcg ttttgggggg cggcgaggcc gagctccgtt atcatgaact
     1021 gcatgaggtc gaggaactgt tcctttgcaa cgcggtggcc ggcgtgcgcc cggtgagcca
     1081 tcttgccggc cgcgcgctgg ccggggcgga ggtcgcgtgc gcgctgcgcg caccgctggc
     1141 tgcggcgggg gtcgcatggc tcggctgatg gtgctgctgc tcggcgcgct gctgctggcc
     1201 ggcggcgcgg cagcgtggtg gggctggcag cagctggata cgcccgggcc gggccgggag
     1261 gacgtcgtgc tggaggtgcc gcagggcgcc tcgctggcgc gcatcgcggc gcgactcgag
     1321 gaggccgggg tggtgcgcca cggccggctg ttcgagtggt acgggcgcgg ctccgggctc
     1381 gcgggccggc tgcgcgccgg cgagtaccgc ttcccggccg ggagcacgcc gcgcgaggtg
     1441 atggcacggc tggtgagcgg cgacgtcgtg ctgcattcgt tcaccatcgt cgagggctgg
     1501 acgttccgcc tgatgcgcac cgccctggag gcccatcccg ccatccgcag cacgctcgag
     1561 gcgctgccgg acgaggaggt catggcgcgg ctaggccggc cgggagagca ccccgaaggc
     1621 tggttcctgc cggagaccta tcgcttcccc cgcggcacca gcgacctgga ggtcctgcgc
     1681 ctgtcgcacc aggcgatgca gcgcgcactc gaggcggcct ggtcgcggcg ccgcgatgcc
     1741 ttgccgttcg agacgcccta cgaagcgctg atcctcgcct cgatcatcga aaaagaaacc
     1801 gggctggccg cggaacgccc cgaaatcgcc ggcgtattcg tgcgccggct ggagcgcggc
     1861 atgcggctgc agaccgaccc gacggtcatc tacggcgccg gacccgggtt cgagggacga
     1921 ctgcgcagca tccacctgga cacggacacc ccgtacaaca cttatactcg cggcggactg
     1981 ccgccgaccc cgatcgcact gccgggacgg gcctcgctcg aggcggcggt gcagccattg
     2041 gagggcaaat cgctgtattt cgtggccacc ggcctgcctg acggcagcca tgtcttttcg
     2101 gaaaccctcg cggagcacaa tcgcgcggtg caacgctact gggccacggt gcgggaacgg
     2161 cggaaccagc ggtgaacgca tccgcgacac cggccacgcc gggccgcttc atcaccctcg
     2221 agggcggtga gggcgtgggc aagagcacca acctggcatt cgtcgctgcc ctgctggaat
     2281 ccgccgggct ggacgtgctg cagacgcgcg agcccggcgg cacgccgctg gccgaacggg
     2341 tacgtgaaat tatcgtctca cccgccggcg aaccggtccc gccgatggcc gagctgctgt
     2401 tgatcttcgc ggcgcgcgcc ctgcacatcg agaacctcat tcgcccggcg ctcaaggcgg
     2461 gccgctgggt gctgtgcgac cgtttcacgg acgccagcta tgcttaccag ggcgcggccc
     2521 gcggcttggg cgaggcgacc gtcggctggc tcgaggcgcg ggtacagggc acgctgcgtc
     2581 cggacctgac gctgttgctg gacgtcgatc ccgccgtcgg gctggcgcgg gcgggcgcgc
     2641 gcggggcggc cgatcgcttc gagcaggagc gcatcgattt cttcgcgcga gtcagggccg
     2701 gttacctggc ccgggccgcg gcggagcctg acaggttccg ggtgatcgat gcggcacagc
     2761 cgctcgatgc cgtgcgccgg gccatcacgc aacacatcaa cgaatacgta caagccaatg
     2821 gaaaaacttg agtttccatg gcatgccgct gcctggaacg agttcgaggc cgcactcgcg
     2881 gccgagcgcc tcgggcatgc ctacctgatc gagggaccgc aaggtctcgg caagctgcac
     2941 ctggcttttc gcatggcggc gcgggtgctg ggcgctccgt ggcctgccgc agacccgccg
     3001 gtgccgctgc aacatccgga cctgaactgg atcacgctgg agaccgacga cgagggcaag
     3061 cagaagaagc agatcggcat ccaccaggtc cgcgaggcct gcgcgaacct ggcgatgacc
     3121 agctactccg gcggctggaa ggtggcggtg gtgtggcccg ccgagcagct caacccgcat
     3181 tccgccaacg cgctgctcaa gacgctcgag gagccggcac cgcgcacact gctgctgctg
     3241 gtgcgcagtc gcctggatac gctgccggcg accatcgcga gtcgctgcca gcgagtccgc
     3301 atcgcgccgc cgccggccgc ggaggcaatc gcctggcttg aggcacgtgc cgacggcggc
     3361 gactggcgac gtctgctgtc gctggccggc gatgcgccgc tgctggcgct gctgctggca
     3421 gaggaggggt tccgggagct cgacggcaag tttgccgccg acctggtcgg catcctcggg
     3481 cgccggcgcg aagcgctgga ggtcgccgcg gagtgggcgc gccagccctt gccgatgacg
     3541 ctgcgctggc tggacgtgtg gacttcggaa ctggtgcgct ggaaggcggc gggcgccatc
     3601 cccgcgctcg agagtgcggt gcagcgggct ttgcaaacgg cgctcgaggg aatacccttg
     3661 caacgccttt tcagatacag ggacgaggtc cgctcagcca tgacccgcaa cgacggcgtt
     3721 ttcaacacgc agatggtgct cgagggactg ctggtcccgt gggccgaccg gctcgagcct
     3781 gtgcgcggcg atgccgccct gggaatgctg agcgaccgat gacgaccagc aagccgggcc
     3841 tcctgacgct gaccatcaag gacaagagcg cgctgtacct cgcgtacatg ccctacgtga
     3901 agaacggcgg cctgttcatt ccgaccagca gcaattaccg gctcggcgac gaggtcttca
     3961 tgctgctgaa cctgatgggt gaggacgaga agattccggt cgccgggcgc gtcatctggg
     4021 tgacgccgaa gggcgcgcag ggcaagcgca ccgccggcat cggcgtgcag ttcagcgagc
     4081 aggatcgcgg cagcacccag cgtcgcatcg agacctacct ggccggcgcg ctcggcgggg
     4141 acaagcccac ccacaccatg tagggccgcc cgcaacggcg cgccccaagg cgccgcaggc
     4201 gccatacgcc aggaattctc agtcggcgga ctcggccgac ttacggcgtt cgccctcggg
     4261 atcggccatc gtcagcccgc ccttgagcac gtgtgcgtcg aagccgcgct cgctgaggat
     4321 gtaggcggcc gcgctgctgc gccgcccggt gtcgcagcac acgacatagc gagtgctctt
     4381 gtccaggctc ttcaacttca gccgaatgaa gt
//
LOCUS       DAOMPG010000204         4388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8313_length_4388_cov_3.104777, whole genome shotgun sequence.
ACCESSION   DAOMPG010000204 DAOMPG010000000
VERSION     DAOMPG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_4388_cov_3.104777"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLS34_10290"
     CDS             complement(<1..991)
                     /locus_tag="PLS34_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRP87892.1"
                     /translation="MIDAFISYSRKDAGRISPLVEALQANGHHVWWDRDIPPGRNFER
                     EIENALASASCILVMLTKNSAESEWVQGESSFGQEHKKLIPVLLDDVRVPLPLRSLQL
                     ADLRGWPDKDQAKDAFRKLVVSLGAHADRHVQPARQFVGRGDALQALQQGFARAARGT
                     GGVMMISGEPGIGKTSCAEEFAQAIEDQGGLVLWGRCYEQSGAPPYWPWVQILRDYAN
                     ANSDDELRVALAASGEAVTTLVPEIAERLGITPKNTDAPSKPEEAFRVFDAVANVFAR
                     HAKLVPLALIIDDLHWADESSLALLEFIAKGLRQQRIYIICTYRDAEVTRKSLL"
     gene            complement(1187..1687)
                     /locus_tag="PLS34_10295"
     CDS             complement(1187..1687)
                     /locus_tag="PLS34_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HRP87893.1"
                     /translation="MTVIRPIILSTLLLCAAPLSAAEWTELSEGIRASDSVLVGGQPS
                     ADVLREAAAAGIKVVVNFRGESEDPGFDEEAVAAELGLTYLRVPVAGAQGLTPENVYL
                     FDAVLDQVGRQPALMHCASGNRVGALHALHAARFGGKDPETAIAIGKAHGLTSLEGEV
                     RKRLEP"
     gene            complement(1789..3156)
                     /locus_tag="PLS34_10300"
     CDS             complement(1789..3156)
                     /locus_tag="PLS34_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRP87894.1"
                     /translation="MKRGSLHRRLLLAAAASIGGALVLSAAGLAVLFQQHVERRIEAE
                     LAAHLEQLLAGIDYRAEGELRLAGRPADPRFEAAFSGLYWQIESGERLLRSRSLWDER
                     LALPADGPADGSVRRHRVPAPGGAELLLLERTIVLPARIGGESLRAGVAIDAAEVRAA
                     RREFLGDLLPYLLLIAVLLWLAAWVQVKVGLSPLAVVRARLAAIHAGEARRLGERFPN
                     EVLPLARQVDVLLEQQERELERARHRAADLAHGLKTPLQVLAGDVETLRARGERELAG
                     EIEEVAVLMRRHVDRELARARMAADRGEALAPAADTVRRVVAVVSRGPAGARLQWRIE
                     VPAALRVRIDPDDLAEALGNLIENAARHARAGVTIQADPQDDIAVLAVLDDGPGIPAE
                     RLEEVLARGARLDSAAAGTGLGLAITRDIAEAWGGSLEIRSGRSGVEARLVLRRGADG
                     TGRSA"
     gene            complement(3153..3818)
                     /locus_tag="PLS34_10305"
     CDS             complement(3153..3818)
                     /locus_tag="PLS34_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP87895.1"
                     /translation="MRILLAEDERRIAAQVTRALEAAGYAVQPVEDGEAAWFQGDTED
                     FDLVVLDLGLPRMDGLAVLKRWRAAGRDMPVLVLTARGSWPERVEGIDAGADDYLPKP
                     FQMQELLARVRALLRRSAGQAAPVIQAAGLELDTRQMRVTRDGVPVVLSPLEYRLLAF
                     LMHQQGRVIPPAELLEHLHGSDDERDVNALEALVARLRKKLGAGAIETRRGFGYCIGR
                     DEA"
     gene            complement(3818..4123)
                     /locus_tag="PLS34_10310"
     CDS             complement(3818..4123)
                     /locus_tag="PLS34_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRP87896.1"
                     /translation="MRKRMTALLALLLIVAGGGLPATADDDHERAWRAVQSGRALPLT
                     EILQRLEGRLGGDVIEVEFEREDGRYVYEFTVITPSGRVREITVDALSAEILEIEDD"
BASE COUNT          667 a         1678 c         1430 g          613 t
ORIGIN      
        1 gcagcaggct cttgcgggtg acctcggcgt cgcggtaggt gcagatgatg tagatgcgct
       61 gctgccgcag gcccttggcg atgaactcga gcagcgcgag ggacgactcg tcggcccagt
      121 gcaggtcatc gatgatcaag gccagcggca cgagcttggc gtgccgcgca aatacgttcg
      181 cgacggcgtc gaatacgcga aaggcctcct ccggcttgct gggcgcatcc gtgttcttcg
      241 gcgtgatgcc cagccgctcg gcaatctcgg gcaccagcgt ggtgacggcc tccccgctcg
      301 ccgccagcgc cacgcgcaac tcgtcgtctg aattggcgtt ggcgtagtcg cgcaggatct
      361 gcacccaggg ccagtagggc ggcgcgcccg actgctcgta gcaccggccc cacagcacca
      421 gcccgccctg gtcctcgatg gcctgcgcga actcctcggc gcagctggtc ttgccgatgc
      481 ccggctcgcc cgagatcatc atcacgccac ccgtgccccg cgcggcgcgc gcgaagccct
      541 gctgcagcgc ctgcagcgcg tcgccgcggc cgacgaactg ccgcgccggc tggacgtggc
      601 ggtcggcgtg cgcgcccagc gagacgacca gcttgcggaa tgcgtccttc gcctggtcct
      661 tgtcgggcca gcccctgaga tcggccagct gcagggagcg cagcggcaac ggcacgcgca
      721 cgtcgtcgag cagcaccggg atgagcttct tgtgttcctg cccgaacgag gactcgccct
      781 gcacccattc ggactcggcg gagtttttcg tcagcatcac gaggatgcac gaggccgacg
      841 cgagcgcatt ctcgatctcg cgctcgaagt tgcggcccgg cggaatgtcg cgatcccacc
      901 agacgtgatg gccgttggcc tgcaacgcct caaccagcgg cgagatgcgg ccggcgtcct
      961 tgcgcgagta actgatgaat gcgtcgatca tgcttgatgc tattcacgcc agccacggcc
     1021 cgtgaatcgt ccatttggac gacccgcggg tcggaccctg gtatcccact gtttttaaga
     1081 gacttatggt caaaaacagg gggattttcg gccttaggat gcgattttcg gcggaaaatg
     1141 gcgtgtctaa ggggtggatc ggcggcccgg cgacccgccc ggcgcgctag ggctcgagcc
     1201 gcttcctgac ctcgccctcc aggctcgtca ggccgtgggc cttgccgatc gcgatcgcgg
     1261 tctcggggtc cttgccgccg aagcgcgcgg catgcagcgc gtgcagcgcg ccgacgcggt
     1321 tgccgctggc gcaatgcatc agcgccggct gccggccgac ctggtcgagc accgcgtcga
     1381 acaggtacac gttttccggc gtcaggccct gtgccccggc caccggcaca cgcaggtaag
     1441 tcagccccag ctcggccgcg acggcttcct cgtcgaagcc gggatcctcg ctctcgccgc
     1501 gaaagttcac caccaccttg atgcccgccg ccgcggcctc gcgcagcacg tccgcagacg
     1561 gctggccacc caccaggacg gagtcgctgg cgcggatgcc ctcggacagc tcggtccact
     1621 cggcggcgga caagggcgcc gcgcacaaca acagggtcga caggatgatc gggcgaatga
     1681 cagtcatgtt gcacctcctt gatgcctgcc cggtatagca gagccgggcg cggagggcca
     1741 agtgctacga ctgcagggcc gccgcgcccg cacgccgcgg accgcagctc acgccgagcg
     1801 tcccgtaccg tccgcgccgc gccgcaacac caaccgggcc tcgacgcccg accgcccgga
     1861 gcggatctcc agcgatccgc cccaggcctc ggcgatgtcg cgagtgatgg ccaggcccag
     1921 cccggtgccc gccgccgcgc tgtccagccg tgcgccgcgc gccagcacct cctccagtcg
     1981 ctccgccgga atgcccggcc cgtcgtcgag cactgccagc acggcgatat catcctgcgg
     2041 gtcggcctgg atggtcacgc cggcccgcgc atgccgggca gcattctcga tcaggttacc
     2101 gagcgcttcc gcgaggtcgt cgggatcgat ccgcacccgc agcgcagctg gcacttcgat
     2161 ccgccactgc agccgcgcgc cggccggccc gcggctcacc accgccacga ccctgcgcac
     2221 cgtgtcggcg gccggggcca gcgcttcccc ccggtcggcc gccatgcgcg cccgcgccag
     2281 ctcacgatcg acgtggcgcc gcatcaacac cgccacctcc tcgatctcac cggccagctc
     2341 acgctcgccc cgggcgcgca gcgtctcgac atcgccggcc aacacctgca acggcgtctt
     2401 caggccgtgc gccaggtcgg cggcgcggtg ccgcgcgcgc tcgagctcgc gctcctgctg
     2461 ctccagcagc acgtccacct gccgcgccag cggcagcacc tcgttcggga agcgctcacc
     2521 caggcggcgt gcctcgcccg cgtggatggc cgccagcctg gcgcgcacca cggcgagcgg
     2581 cgacaagccc accttgacct gcacccaggc cgccagccac agcagcaccg cgatcagcag
     2641 gagataaggc agcaagtcac cgagaaattc gcgacgggcg gcgcgcacct ccgccgcatc
     2701 gattgccacg ccggcccgca gcgactcacc gccgatccgc gccggcagca caatggtgcg
     2761 ctccagcagc aacaactccg caccgccggg cgcgggcacg cgatgacggc gcacgctgcc
     2821 gtccgccggc ccatccgccg gcaacgccag ccgctcatcc cagagcgagc gcgagcgcag
     2881 cagccgttcg cccgactcga tctgccagta aagcccggag aacgcagcct cgaagcgcgg
     2941 gtccgcgggc cggccagcaa gccgcagctc gccttcggca cggtagtcga tccccgccaa
     3001 cagctgctcc aggtgcgccg ccagctcggc ctcgatgcgc cgctcgacgt gctgctggaa
     3061 cagcaccgcc aggcccgccg ccgacaacac cagcgccccg ccgatggacg cggccgccgc
     3121 gagcagcagc ctgcggtgca gcgaaccgcg tttcatgcct cgtctcggcc gatgcagtag
     3181 ccgaagccgc gccgcgtctc gatcgcgccc gcgccgagct tcttgcgcag gcgcgccacc
     3241 agcgcctcga gggcgttgac gtcgcgctcg tcgtcactgc cgtgcaagtg ctcgagcagc
     3301 tccgcgggcg gaatcacccg gccctgctgg tgcatcagga aagccagcag ccggtactcc
     3361 agcggcgaca gcaccaccgg cacgccgtcg cgggtgaccc gcatctggcg cgtgtccagc
     3421 tccagcccgg cggcctggat gaccggtgcc gcctggcccg ccgagcggcg cagcagtgcc
     3481 cgcacgcggg ccagcagttc ctgcatctgg aagggcttgg gcaggtaatc gtcggcgccg
     3541 gcgtcgattc cttcgacgcg ctccggccac gagccgcgcg ccgtcagcac cagcaccggc
     3601 atgtcgcgcc ccgcggcgcg ccagcgcttc agcacggcca gcccgtccat gcgcggcaga
     3661 ccgaggtcca ggaccaccag atcaaagtcc tcggtgtcgc cctggaacca ggcggcctcc
     3721 ccgtcctcca ccggctgcac ggcgtagccg gccgcctcca gcgcgcgcgt cacctgcgcc
     3781 gcgattcgcc gttcgtcctc ggcgagcagg atgcgcatca gtcgtcctcg atttccagga
     3841 tctccgcgct gagggcgtcg acggtgatct cccgcacgcg gccggacggc gtgatcaccg
     3901 tgaactcgta cacgtagcgg ccgtcctcgc gctcgaactc cacctcgatg acgtcgccgc
     3961 cgaggcgtcc ttccaggcgc tgcaggatct ccgtcagcgg cagcgcgcgg ccggactgga
     4021 ccgcccgcca ggcgcgctcg tggtcgtcgt cggccgtcgc cggcaggccg ccgccggcga
     4081 cgatcagcag cagcgccagc agcgccgtca ttcgcttgcg catggttgtc atacctcatc
     4141 aataccgcag gcggcctgac agttcgctga catgggtcat caggggcggg tcagcggggt
     4201 cgtgccattg tcgcctcacg ggacggcagc agccgcaacc cggcacacat caacacggag
     4261 acaagtacat gcgcaagata ctgaccctgg ccgtattcgg actgaccttg gcggccggct
     4321 acagcctcgc cgcggaccag cgcccgggtg ccgacgcccc gcataccgag tggctgccga
     4381 tcgagcag
//
LOCUS       DAOMPG010000205         4371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8351_length_4371_cov_3.547729, whole genome shotgun sequence.
ACCESSION   DAOMPG010000205 DAOMPG010000000
VERSION     DAOMPG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8351_length_4371_cov_3.547729"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLS34_10315"
     CDS             <1..543
                     /locus_tag="PLS34_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRP87897.1"
                     /translation="GHEVDFTIADFAADRRAPTPSGAAEIAVPDAAEWLARLNKDAGR
                     LRNAMAQRLRVKAERLAWQTRRLAVAHPGHRLRPHGQRLDELERRLATVLGRTLQALR
                     ARCEAAARTLHAVSPLATLGRGYAIVTRADDGAILRAAAEVAVGERIRVRLAAGSLTA
                     AVADRDPAGGDARPDQDRSK"
     gene            complement(525..1577)
                     /locus_tag="PLS34_10320"
     CDS             complement(525..1577)
                     /locus_tag="PLS34_10320"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRP87898.1"
                     /translation="MHRGGLLPRLDIAYETWGELDARAGNAVLLFTGLSPSAHAASSP
                     EDPASGWWEEMVGPGRPIDTRRWFVVCVNSMGSCFGTTGPASIDPRNGEPYRLRFPVL
                     TVEDIAAAGREVVRHLGIQKLHTVIGPSLGGMSALAFCTLFPEMSHGLVAISSTARAN
                     PFAIALRSLQREIIRSDPAWMSGNYPQDRPPLVGMPLARKVGMITYRSAEEWDQRFGR
                     ERVGGEHDAGDQFGVDFEVESYLEHHANKFIGQFDPNCYLYLSRAMDLFDVAEHSGCV
                     VTELSKPGVDRALVIGVETDFLFPLHEQEQMAECLERPDREVEFVALPSLHGHDSFLV
                     DMDGFRPVIARYFDRS"
     gene            complement(1699..3096)
                     /gene="der"
                     /locus_tag="PLS34_10325"
     CDS             complement(1699..3096)
                     /gene="der"
                     /locus_tag="PLS34_10325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRP87899.1"
                     /translation="MIPVVALVGRPNVGKSTLFNFLTRTRDALVADLPGLTRDRQYGF
                     GRVGPVPYIVVDTGGLTGTKDELDGLMARQTLRALDEADVVLFMVDAREGLTAGDEQV
                     ATLLRRRGRPVTLVVNKAEGQSPAMVVAEFHRLGMGEPSVISSAHGDNVRALMDAVLA
                     PFPGPDESDVPERAEETIRVAVIGRPNVGKSTLINRMIGEDRLVAYDLPGTTRDAVEV
                     PFERDGQRYTLVDTAGVRRRSRVADAVEKFSIIKTLGAIDAASVVIAVLDARDGITDQ
                     DASLLGLAAERGRAMVIAVNKWDGLPPDQRDTVRRQLDLKLPFVDYAPIQFISALHGT
                     GVGDLFALVAASHAAAHRDLPTPELTRILQDAVSAHQPPLSKGRRIKLRYAHQGGRNP
                     PVIVVHGSQAELLPDTYRRYLANIFRKSFKLQGTPVRIELRSGDNPYAGRRNPLTPRQ
                     RKKRERLMRHVKKGR"
     gene            complement(3109..4278)
                     /gene="bamB"
                     /locus_tag="PLS34_10330"
     CDS             complement(3109..4278)
                     /gene="bamB"
                     /locus_tag="PLS34_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HRP87900.1"
                     /translation="MSLSPKQAVLWTMLLPLVLGGCGIFGSKEEPIEPPAELTDIEPV
                     LTVRKAWDTRVGSGSELLNLALRPAVEGGRVYAAGRNGRVHALDLETGRSQWTVRTEL
                     ALSAGPTVGHGLVLVGGTGGMLVALAMDDGAERWRSQLAGEVLAPPAVSPTVVVVRTV
                     DGRLHGLSAADGRELWMVEQQPPRLTLRGTSAPTIAGNVVVTGFDTGRIGAYLLQDGE
                     AQWENVIAVGRGRTEIERLADVDATPQVIGQDLYAVSYGGRLANLAIESGQILWTAEM
                     SSYNGLSLDWTSVYVAAADGEVVALNRSSGAELWRQGALRMRRLTAPTPVGQSVVVGD
                     FEGWLHWLDAFTGAIQARYRAGKAAIVTAPVSGGELLIVQDEDDRVYALRAGPRG"
BASE COUNT          626 a         1614 c         1521 g          610 t
ORIGIN      
        1 ggccacgagg tggacttcac catcgccgat ttcgccgccg atcggcgcgc gccgacgccc
       61 tcgggggccg ccgaaatcgc cgtgccggac gccgccgagt ggctcgcgcg gctgaacaag
      121 gacgccggac gactgcgcaa tgccatggcg cagcggctgc gcgtgaaggc ggagcggctc
      181 gcctggcaga cgcgccggct ggcggtggcc catcccggcc accgcctgcg cccgcacggc
      241 cagcggctgg atgaactgga gcgccggctg gcgactgtgc tgggccgcac gctgcaggcc
      301 ctgcgggccc gttgcgaggc cgcggcgcgc accctgcacg cggtcagccc gctggcgacg
      361 ctggggcgcg gctacgccat cgtgacgcgt gccgacgacg gcgccatcct gcgcgccgcg
      421 gccgaagtgg cggtcggcga acgcattcgc gtcaggctcg ccgccgggtc gctcacggcc
      481 gcggttgcgg accgggaccc ggcgggcggc gacgcgcgcc ccgatcagga ccggtcgaaa
      541 tagcgggcga tcaccggccg gaaaccgtcc atgtcgacca ggaaggaatc gtggccgtgc
      601 agggacggca gggccacgaa ctccacctcg cgatccggcc gctcgaggca ctcggccatc
      661 tgctcctgtt cgtgcagcgg gaacaggaag tccgtctcca cgccgatcac cagcgcccgg
      721 tcgacgcccg gcttggacag ctcggtgacg acgcagccgc tgtgctccgc cacgtcgaac
      781 aggtccatgg cgcgcgacag gtacaggtag cagttcgggt cgaactggcc gatgaacttg
      841 ttggcgtgat gctccaggta ggactcgacc tcgaagtcca cgccgaactg gtcgccggcg
      901 tcatgctcgc cgccgacccg ctcgcgtccg aaccgctggt cccactcctc ggccgagcga
      961 taggtgatca tgccgacctt gcgcgccagg ggcatgccca ccagcggcgg acgatcctgc
     1021 gggtagttgc cgctcatcca cgcggggtcg gagcggatga tctcgcgctg cagcgagcgc
     1081 aacgcgatcg cgaaaggatt ggcgcgcgcg gtcgacgaga tcgccaccag gccgtggctc
     1141 atctccggaa acagcgtgca gaaggccagc gcggacatgc cgcccagtga cggcccgatg
     1201 accgtgtgga gcttctggat gccgaggtgc ctgaccactt cacggcccgc cgcagcgatg
     1261 tcctcgacgg tgagcaccgg gaacctcagg cggtagggct caccgttgcg cggatcgatg
     1321 gaggcggggc cggtcgtgcc gaagcagctg cccatggaat tcacgcagac gacgaaccag
     1381 cgccgggtgt cgatcggtcg ccccggcccg accatctctt cccaccagcc ggacgcggga
     1441 tcttccggcg aggacgccgc gtgcgccgac ggcgacaggc cggtgaacag cagcaccgcg
     1501 ttgccggcgc gcgcatccag ctcgccccag gtctcgtagg cgatgtcgag gcgcggcaac
     1561 aggccgccgc gatgcatccg gaacggcccg tcccggcgca cgaagcggcg tgcgccggtc
     1621 gcggcccggg cctcgctggg cgcgaacggc ggcgccttgc tgacttgctg gctcatgacc
     1681 tcgcgtcctc cggcatgctc aacggccctt cttcacgtgg cgcatcagcc gctcgcgctt
     1741 cttccgctgg cgcggcgtga gcggattcct gcgccccgca tagggattgt cgcccgagcg
     1801 cagctcgatg cgcaccggcg tgccctgcag cttgaaactc ttgcgaaaaa tattggccag
     1861 gtagcgtcgg taggtgtccg gcagcaactc cgcctggctg ccgtgcacca cgatcaccgg
     1921 cgggttgcgg ccgccctggt gagcatagcg cagcttgatg cgccggccct tcgacagcgg
     1981 cggctggtgc gcgctgacgg cgtcctgcag gatccgcgtc agctcgggcg tcggcaggtc
     2041 gcggtgggcg gcggcatgac tcgccgcgac cagcgcgaac aggtcgccga cgccggtgcc
     2101 gtgcagcgcc gagatgaact ggatgggcgc gtagtccacg aacggcagct tcaggtccag
     2161 ctggcggcgc accgtgtcgc gctggtcagg cggcaggccg tcccacttgt tgacggcgat
     2221 caccatcgcg cggccgcgct cggcggccag tcccagcagg ctggcatcct ggtcggtgat
     2281 cccgtcgcgc gcgtccagca ccgcgatcac caccgacgcg gcgtcgatgg cgccgagcgt
     2341 cttgatgatg ctgaatttct cgacggcgtc agccacccgg gagcggcgcc gcacgccggc
     2401 cgtgtcgacc agcgtgtagc gctggccgtc gcgctcgaac gggacttcca cggcgtcgcg
     2461 cgtggtgccg ggcaggtcgt aggccaccag ccggtcctcg ccgatcatgc gattgatgag
     2521 cgtcgacttg ccgacgttcg gccggccgat cacggcgacg cggatcgtct cctcggcgcg
     2581 ctcgggcacg tcgctctcgt cgggacccgg gaagggcgcg agcacggcgt ccatcagcgc
     2641 ccgcacgttg tcgccgtggg ccgaggagat caccgacggc tcgcccatgc cgagccggtg
     2701 aaactccgcc acgaccatcg ccggcgactg gccctcggcc ttgttgacca ccagcgtcac
     2761 cgggcggccg cgcctgcgga gcagggtcgc gacctgctcg tcgccggccg tcaggccttc
     2821 ccgcgcatcc accatgaaca gcacgacgtc ggcctcgtcg agcgcgcgca gcgtctggcg
     2881 cgccatcagg ccgtcaagct cgtccttggt acccgtcagc ccgccggtgt ccaccacgat
     2941 gtagggcacc ggcccgacgc gcccgaaacc atactgtcga tcccgcgtca ggccgggcag
     3001 gtcggccacc agcgcgtcgc gcgtgcgcgt caggaaattg aacagggtcg acttgccgac
     3061 attcggcctg ccgaccaggg cgacgacggg aatcatgggc gcgaggcctc agccccgtgg
     3121 ccccgcgcgc agggcgtaca cccggtcgtc ctcgtcctgg acgatgagca gctcgccgcc
     3181 gctgaccggc gccgtgacga tggccgcctt gccggcgcgg tagcgcgcct ggatggcgcc
     3241 ggtgaaggcg tccagccagt ggagccagcc ctcgaagtcc cctaccacga cgctctgccc
     3301 gaccggcgtc ggcgccgtca gccgccgcat ccgcaacgcg ccctggcgcc acaactccgc
     3361 gccgctggag cggttcaggg cgaccacctc gccgtcggcg gccgccacgt agacgctggt
     3421 ccagtcgagg ctcaggccgt tgtagctcga catctcggcg gtccagagga tctggcctga
     3481 ctcgatcgcc aggttcgcca gccggccgcc gtagctcacc gcgtacaggt cctggccgat
     3541 gacctgcggg gtggcgtcga cgtccgccag gcgctcgatc tcggtgcggc cgcggccgac
     3601 ggcgatgacg ttctcccact gcgcctcgcc gtcctgcagc aggtaggcgc cgatgcgccc
     3661 ggtgtcgaaa cccgtcacca cgacgttgcc ggcaatggtc ggcgccgacg tcccgcgcag
     3721 cgtcaggcgc ggcggctgct gttccaccat ccacaattcg cggccgtcgg cggccgacag
     3781 gccgtgcagc cggccgtcca cggtgcgcac cacgaccacc gtcggcgaga cggccggcgg
     3841 tgcgagcacc tcgccggcca gctgtgagcg ccagcgctcg gcgccgtcgt ccatggcgag
     3901 cgcgaccagc atcccgccgg tgccgcccac cagcacgaga ccgtgcccga ccgtcggacc
     3961 cgccgacagt gccagctcgg tccgcaccgt ccactggcta cggcccgtct cgaggtccag
     4021 cgcatgcacg cgaccgttgc ggcccgccgc atagacgcgc cccccctcga ccgcgggacg
     4081 cagtgcgagg ttgagcaact ccgagcccga gccgacccgc gtgtcccagg ccttgcgcac
     4141 cgtcagcacg ggctcgatgt cggtgagctc cgccggcggc tcgatcggct cctccttgct
     4201 gccgaagatg ccgcagccgc ccagcacgag cggcaggagc atcgtccaca ggactgcctg
     4261 tttcgggctc aggctcacga agcgtcgcct gccgcgccgt ctgcgagtgc ctcgagcttg
     4321 agccgcacga attcctcgtc gatgatgccg ctgaaggcgg ccgccttgcc g
//
LOCUS       DAOMPG010000206         4346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8413_length_4346_cov_3.663948, whole genome shotgun sequence.
ACCESSION   DAOMPG010000206 DAOMPG010000000
VERSION     DAOMPG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8413_length_4346_cov_3.663948"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PLS34_10335"
     CDS             <1..1387
                     /locus_tag="PLS34_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87901.1"
                     /translation="PRLTELFFSCDAEGFRAEYERLEADPSFHKEWFQPRELLLTWLN
                     EALETGVAYLANVEEMNRHTPFKEPIHSSNLCLEICEPTRPYEDMRDLYSDREVGWID
                     VELLGGERRRLGFNDALTRNGQTIFAGEIREGEQVTDAKGETFVVSRLLATHAEPEVA
                     LCSLGAIACDNIPDDDDAAYEEAMYLTLKMIDYCIEHSHYALPHVGVTARARMNAGIG
                     LMGVATVMARKRLRFDTPEGLAELHRIAERHMYFAIKASLRLARERGLPRWIHKTRWV
                     DGWLPIDSYNRNVDSLGDFSNRYDWEALRHEIIATGGIGHSCLVAFMPGESSSKALGQ
                     PNGLYPVRDATLGKTDGNVTIRWAAPDSDDPACLYQSAWDIREVDMIKAYAVFQKFTD
                     QGISADLWRRIRGDEKVSSKELLEQYFAMFKYGLKTRYYYNSNVASGLSIHDTARISD
                     GHDGACEGCTL"
     gene            1478..2590
                     /locus_tag="PLS34_10340"
     CDS             1478..2590
                     /locus_tag="PLS34_10340"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015969115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRP87902.1"
                     /translation="MTALPTKIFNTEKTDYETPEIILGSDPGLFDTINKHYPALWKLY
                     KTLKSQDWDENEFDYAPCNVQFKTCDKATYDMMVKTLAWQWEADSVAARSIAGVLAPV
                     CTSSEAWAGWLEISRNEVVHAATYSEIVRNSFDDPDDILEEILAVKEAMARLKTVGEA
                     FDDALAVSLDYARGARPNDQATYNAIYLFIVTLLCMERIQFMASFAVTFGVCESTGLF
                     QPIGKAIQKIAQDEFEVHVQYGKEALKSLYKTERGRRAFADCRGKIIAIINEVVASEV
                     EWTDYAFSEGRELTGVTCDMMKDWVYFSATDVISFFGLEEAASFPLVPNNPLAYMENW
                     LDMSKIQASNQEEDTAQYKVGVMKRDDEDEVFAVDF"
     gene            2773..2997
                     /locus_tag="PLS34_10345"
     CDS             2773..2997
                     /locus_tag="PLS34_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016442131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRP87903.1"
                     /translation="MTVTVYSKPDCPQCDSTRRDLEILGIEYALVDLMQDRAALQRLV
                     GMGYRSAPVVETDRGSWSGYDRERIKALLA"
     gene            complement(3054..>4346)
                     /locus_tag="PLS34_10350"
     CDS             complement(3054..>4346)
                     /locus_tag="PLS34_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87904.1"
                     /translation="KAAPPGPAGAWQGELGVLYLAADGRGALLRGSDDDAEEDDEAQE
                     TYAMLLEWRADGDDLELLNPQAVTAPRERFSDEEVEEFLESFGSYYAGLPPQDLVQRI
                     RDEYEATARSDQSRWMRTVLLQDGANALLLDRERLLAQAQRWSGEGALQLAPAFWRSS
                     GEDSPLAAMDPRAPGYALADPLHPGVPKALRDLLRVQAIETRVLARLDADAPLQWRHH
                     SAQVRLRLDRGSDDGLIEGMSLYGLPPNEGWYANLNQVDAGESVATMYLSRFAPDDEV
                     ALPARGTAFSTRRDAPLRCSVDASAAVRAAITAVSAPADGVTLDADGYAYLELEIDLG
                     RGEGLAVGDRVYLEQDADGEARIVGEGRVRRVEAGSARVLWRTYRWLGAWEEDEDAGK
                     GAAPVKVERSALPAVGQHLITPAWRQVESDLFGALRRR"
BASE COUNT          754 a         1577 c         1360 g          655 t
ORIGIN      
        1 gccgcggctg accgagctct ttttcagctg cgacgccgag ggcttccgcg ccgagtacga
       61 gcgcctggaa gccgatcctt ccttccacaa ggaatggttc cagccgcgcg agctgctgct
      121 cacctggctc aacgaggcgc tggaaaccgg cgtggcctac ctcgccaacg tcgaggagat
      181 gaaccgccac acgccgttca aggagccgat ccactcctcc aacctgtgcc tggaaatctg
      241 cgagccgacc cggccgtatg aagacatgcg cgacctgtac tccgaccgtg aagtgggctg
      301 gatcgacgtc gagctcctgg gcggcgagcg ccgccggctc ggcttcaacg atgcgctcac
      361 ccgcaacggc cagacgatct ttgccggcga gatccgcgag ggcgagcagg tcaccgatgc
      421 aaagggcgaa accttcgtcg tcagccgcct cctcgccacc cacgccgagc cggaggttgc
      481 gctgtgctcg ctgggcgcga tcgcctgcga caacattccc gacgacgacg atgccgccta
      541 cgaagaggcg atgtatctga cgctgaagat gatcgactac tgcatcgagc acagccacta
      601 cgccctgccg cacgtgggcg tcacggcgag ggcgcgcatg aacgccggta tcggtctgat
      661 gggcgtggcc acggtgatgg cgcgcaagcg gctgcgcttc gatacgcccg agggcctggc
      721 cgagctgcac cgcatcgccg agcggcacat gtacttcgcc atcaaggcct cgctgcgcct
      781 ggcgcgcgag cgcggcctgc cgcgctggat ccacaagacc cgctgggtcg acggctggct
      841 gccgatcgac agctacaacc gcaacgtcga ttcgctgggc gacttctcca atcgctacga
      901 ctgggaggcc ctgcgccacg agatcatcgc caccggcggc atcggccatt cgtgcctcgt
      961 cgccttcatg ccgggcgagt cctcctccaa ggcgctgggc cagcccaacg gcctgtatcc
     1021 ggtgcgcgac gccacgctgg gcaagaccga cggcaacgtc accatccgct gggccgcgcc
     1081 cgacagcgac gatcccgcct gcctctacca gtcggcctgg gacattcgcg aggtcgacat
     1141 gatcaaggcc tatgcggtgt tccagaagtt caccgaccag ggcatctcgg ccgacctgtg
     1201 gcggcgcatc cgcggcgacg aaaaggtctc cagcaaggag ctgctggagc agtacttcgc
     1261 catgttcaag tacggcctca agacccgcta ctactacaac tccaacgtcg cctcgggcct
     1321 gtcgatccac gacaccgccc gcatcagcga cggccacgac ggcgcctgcg aaggctgcac
     1381 cctgtagccc cccattcccc attccccatt ccccattccc caatccccat tccccaatcc
     1441 ccattcccca atccccaatc cccaatcccg aaccgccatg accgccctgc ccaccaaaat
     1501 cttcaacacc gaaaaaaccg actacgaaac cccggaaatc atcctcggct ccgaccccgg
     1561 cctgttcgac accatcaaca agcactaccc ggcgctgtgg aagctctaca agaccctcaa
     1621 gagccaggac tgggacgaga acgagttcga ctacgcgccc tgcaacgtgc agttcaagac
     1681 ctgcgacaag gccacctacg acatgatggt caagacgctg gcctggcagt gggaagccga
     1741 cagcgtcgcc gcgcgctcca tcgccggcgt gctggccccc gtgtgcacca gctcggaggc
     1801 ctgggccggg tggctggaga tcagccgcaa cgaggtcgtg cacgcggcca cctattcgga
     1861 aatcgtgcgc aacagcttcg acgacccgga cgacatcctc gaggagatcc tcgcggtcaa
     1921 ggaggcgatg gcgcggctga agaccgtggg cgaggccttc gacgacgcgc tcgcggtcag
     1981 cctcgactac gcccgcggcg cgcggccgaa cgaccaggcc acctacaacg ccatctacct
     2041 cttcatcgtc accctgctgt gcatggagcg catccagttc atggcgtctt tcgcggtgac
     2101 cttcggcgtg tgcgagtcca ccggcctgtt ccagccgatc ggcaaggcga tccagaagat
     2161 cgcccaggac gagttcgagg tgcacgtgca gtacggcaag gaggcgctga agtcgctcta
     2221 caagaccgag cgcggccgcc gcgccttcgc cgactgccgc gggaagatca tcgccatcat
     2281 caatgaagtc gtcgccagcg aagtcgagtg gaccgactac gccttctccg agggccgcga
     2341 gctgaccggc gtgacctgcg acatgatgaa ggactgggtc tacttcagcg ccaccgacgt
     2401 catcagcttc ttcggactgg aagaggccgc gagcttcccg ctggtgccga acaacccgct
     2461 ggcctacatg gagaactggc tcgacatgag caagatccag gcctccaacc aggaagagga
     2521 caccgcccag tacaaggtcg gcgtgatgaa gcgcgacgac gaggacgagg tgttcgccgt
     2581 cgatttctga ggccccgtat ggctttccca tcgcaggagc gggcttgccc gcaaagagtc
     2641 ccgcgctatc gcatatcaca ttcgcgacca aggtcgctcc tacaaaaacc accaggctct
     2701 gtggaagcgg actcgcccgc gaatgcaccc ttccacaggc ccgttcaccc aaactctcac
     2761 ggagcacaca ccatgaccgt caccgtctac tccaaacccg actgcccgca gtgcgattcc
     2821 acccgccgcg acctggaaat cctgggcatc gagtacgccc tggtcgacct gatgcaggac
     2881 cgcgccgcgc tgcagcgcct ggtcggaatg ggctaccgca gcgccccggt cgtggaaacg
     2941 gaccgcggca gctggagcgg ctacgaccgc gagcggatca aggcgctgct ggcctgaagc
     3001 ccggtcggcc ccgcgccgca gcgccgaaac gtgcgggccg cgtgatcctg cgctcagcgc
     3061 cgccgcagcg cgccgaacag gtcgctctcc acctgccgcc aggccggcgt gatcaggtgc
     3121 tggccgacgg ccggcagggc cgaccgctcc accttcaccg gtgccgcgcc tttacccgcg
     3181 tcttcatcct cctcccaggc accgagccag cggtaggtgc gccacagtac gcgcgcgctg
     3241 ccggcctcga cccggcgcac gcggccttcg ccgacgatgc gggcctcgcc gtccgcatcc
     3301 tgttccaggt agacccggtc gcccaccgcc aggccctcgc cgcggcccag gtcgatttcc
     3361 agttcgaggt aggcgtagcc gtcggcgtcc agggtcaccc cgtccgccgg cgcggaaacg
     3421 gcggtgatcg cggcgcgcac cgcggcgctg gcgtccacgc tgcagcgcag cggcgcgtcg
     3481 cggcgggtgc tgaaggcggt gccccgggcg ggcagcgcca cctcgtcgtc cggcgcgaag
     3541 cggctcagat acatcgtggc gaccgattcg cccgcgtcca cctgattcaa gttcgcgtac
     3601 cagccctcgt tcggcggaag cccgtacagg ctcatgcctt cgatcaggcc gtcgtccgat
     3661 ccgcggtcca gccgcagccg cacctgcgcc gagtggtggc gccattgcag cggcgcgtcc
     3721 gcgtcgaggc gcgccagcac gcgggtttcg atggcttgga cgcgcagcag gtcgcgcagc
     3781 gccttgggca cgccggggtg cagcgggtcg gcgagcgcat agccgggggc acgcgggtcc
     3841 atcgcggcca gcggtgaatc ctcgccggac gagcgccaga acgccggcgc gagctgcagc
     3901 gcgccctcgc cgctccagcg ctgcgcctgg gccagcaggc gctcgcggtc gagcaggagg
     3961 gcgttggcac cgtcttgcag cagcaccgtg cgcatccagc gtgattgatc ggagcgggcc
     4021 gtcgcctcgt attcgtcgcg gatgcgctgc accaggtcct gcggcggcag gccggcgtag
     4081 tagctgccga aggattccag gaactcctcg acctcttcgt cggagaatcg ttcgcgcggc
     4141 gcggtgacgg cctgcgggtt cagcagttcg aggtcgtcgc cgtccgcccg ccattccagc
     4201 agcatggcgt aggtttcctg cgcttcgtcg tcttcttccg cgtcgtcgtc gctgccgcgg
     4261 agcagggcac cgcggccgtc ggcggccagg tacaggacgc ccaactcgcc ctgccaggca
     4321 ccggccggac ccggcggcgc ggcctt
//
LOCUS       DAOMPG010000207         4309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8505_length_4309_cov_4.588858, whole genome shotgun sequence.
ACCESSION   DAOMPG010000207 DAOMPG010000000
VERSION     DAOMPG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8505_length_4309_cov_4.588858"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1248)
                     /locus_tag="PLS34_10355"
     CDS             complement(1..1248)
                     /locus_tag="PLS34_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP87905.1"
                     /translation="MDLIRSRAGRKSLFALLYLAEGAPIGFIWWAVPSRLREAGVPVG
                     TIALLTSVVILPWAFKFLWAPLVDVLRGARWGRRAWIVSAQLLMGLTLLPLAFVPWED
                     SIDLLLAILLLHAFAAATQDVAIDAWAISITTPEERGGINGWMQSGMLFGRWLFGAGF
                     LIVAHRVDDTTAVLAMVGVIWTTALVVVLASDREDDCVGSPLLASVRARLGEFLATLR
                     SALGKRETWLGIALAMIAFAGFKAVGDLAGPFLVDQGLAQAQIGHFFTGALVSMIVGA
                     LVGGWTADRFGNVPAIRGFVALAVVVILATSAASIGRGDGKVVVILGLLAVYLCAGLI
                     TVSAYALLMQLTDPKLGATQFSAYMGAVNACESWSGFTAGQLAQRFDYPVAFAAVALV
                     SLLALPLLARIRRAAPPSRGIRC"
     gene            complement(1273..1349)
                     /locus_tag="PLS34_10360"
     tRNA            complement(1273..1349)
                     /locus_tag="PLS34_10360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1313..1315),aa:Pro,seq:ggg)
     gene            complement(1455..2303)
                     /locus_tag="PLS34_10365"
     CDS             complement(1455..2303)
                     /locus_tag="PLS34_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRP87906.1"
                     /translation="MRRLFLPFLFFTVAFLGVGCSRQAPPTEPTAAADTPVTAEAPRE
                     VPGDADCRLTMGWDPWPPFHYEDVGGQLAGFDVELLRAMAADAGCGLVFERDSWAALL
                     AKLREGRIDLLTGATITAARQEFAVFSAPIRREEFGLYVRAGQIPAWPADEPRALMEQ
                     GFRLGVIGAYVYDPAIEAILSEPAYTEQLVYAHFGEALGGLLLDGEIDGFVEEVYAAA
                     SILRRLGLADAITRHPMRLGEGTDVHIMYSRASVAPEQVERLGEALSKLKSSGAYEAL
                     RVQYLE"
     gene            complement(2410..2769)
                     /locus_tag="PLS34_10370"
     CDS             complement(2410..2769)
                     /locus_tag="PLS34_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRP87907.1"
                     /translation="MLEPGNNDELPAIPGKRYFTIGEVSELCGVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRRDVLLIRQIRSLLYEEGFTIGGARQRLTGDDAKEDLTQSVQIV
                     RQLRVELEEILKILRSK"
     gene            complement(2750..3049)
                     /gene="ihfA"
                     /locus_tag="PLS34_10375"
     CDS             complement(2750..3049)
                     /gene="ihfA"
                     /locus_tag="PLS34_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRP87908.1"
                     /translation="MALTKAEMAEALFQDMGLNKREARELVEMFFEELRTALANGEQV
                     KLSGFGNFDLRDKNRRPGRNPKTGEEIPITARRVVTFRPGQKLKTRVEAYAGTGE"
     gene            complement(3051..>4309)
                     /locus_tag="PLS34_10380"
     CDS             complement(3051..>4309)
                     /locus_tag="PLS34_10380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRP87909.1"
                     /translation="VERATALLAELAGGQPGPVIERVAEQYLPERSPVRLRRARLAML
                     LGHELPDEEIERILEGLGIRLAPSDEGWEAVPPSHRFDIAIEADLIEEVARVHGYDRL
                     PEARGAGAAVLGEASEARVPGVRLADMLAARGYQEVVTYSFVDPQLQTKLFPESRALP
                     LSNPISTELSDMRVSLWPGLVHTLKRNLSRRQSRVRIFEQGLRFISQDNELKQLTTIA
                     GLIAGARLPEQWGAPAVPADFHDAKGDVEALLGLSNAEWVFAPAEHSALHPGQCARIL
                     RDGREAGWIGALHPRLVRALDLERAPLLWELDEATCFAAELPAFREISRFPAIRRDVA
                     VVIDEAVPVQAMVDAIRSAGGALLTESKVFDVYSGERIDSGRKSVAFGLILQESSRTL
                     TDEEADRVVAAVVARLASEFDARMRD"
BASE COUNT          767 a         1502 c         1384 g          656 t
ORIGIN      
        1 ctagcagcgg atcccccgcg acggcggcgc ggcccgccgg atccgcgcca gcaaaggcaa
       61 cgccagcagc gagacgagcg cgacggccgc gaaggcgacc ggatagtcga agcgttgcgc
      121 caactgcccg gccgtgaagc ctgaccacga ctcgcaggcg ttgaccgcgc ccatgtaggc
      181 cgagaactgc gtcgccccga gtttcgggtc agtgagttgc atcagcagcg cataggccga
      241 caccgtaatg agaccggcgc acaggtacac cgcgagcaga ccgaggatca ccacgacttt
      301 gccgtctccg cgacctatcg acgcagcgct cgtcgcgagg atcaccacca ccgcgagcgc
      361 cacgaagcct cggatggcgg gcacattgcc gaaccggtcg gccgtccagc cgccgaccag
      421 cgcaccgacg atcatggaca ccagtgcgcc ggtgaagaaa tgcccgatct gcgcctgggc
      481 cagtccctgg tcgacgagga acggtcccgc cagatcgccg accgccttga agccggcaaa
      541 tgcgatcatc gccagggcga tcccgagcca ggtctcgcgc ttgcccaagg cgctgcgcag
      601 cgtagcgagg aactccccaa ggcgtgccct gaccgaggcc agcaggggac tcccgacgca
      661 gtcatcctca cggtccgagg cgagcaccac caccagcgca gtcgtccaga tcacgccgac
      721 catggcgagc acggccgtcg tgtcgtcgac ccggtgtgcg acgatcagga acccggcgcc
      781 gaacagccag cggccgaaca gcatgcccga ctgcatccag ccgttgatgc cgccgcgctc
      841 ctccggcgtc gtgatgctga tcgcccacgc atcgattgcg acgtcctggg tggcggcggc
      901 gaaggcatgt aacagcagga tggccagcag caggtcgatc gagtcttccc agggcacaaa
      961 ggccagcggc agcagggtca ggcccatcag cagttgcgcc gacacgatcc aggcacgccg
     1021 tccccagcgc gccccgcgca gcacgtcgac cagcggcgcc cacaggaact tgaacgccca
     1081 gggcaggatc accaccgagg tcagcagcgc aatggtgccg accggcacgc ccgcctcccg
     1141 caaccggctg ggcaccgccc accagatgaa gccgatgggc gcgccctccg cgaggtagag
     1201 caaggcaaag aggctcttgc ggcctgcgcg ggatcggatc aggtccatgc gtgccggaac
     1261 taggagatcg aatggtcggg gcgagaggat ttgaacctcc gaccccctgc accccatgca
     1321 ggtgcgctac caggctgcgc cacgccccga ccgaagggaa tctggacgcc tcagggatgc
     1381 gtccaagggg acgcgcatga taaccgatgc ctctgccccg gcaaagggcg gtgtgcctcg
     1441 cctggggccc ggcatcactc gaggtattgc acccgcaggg cctcgtaggc gcctgatgac
     1501 ttcagcttcg acagggcttc cccgaggcgc tccacctgtt ccggcgccac gctggccctg
     1561 ctgtacatga tgtggacgtc agtgccttcg ccgagccgca tcgggtggcg cgtgatcgca
     1621 tcggcaagcc cgagacgacg gaggatactg gctgcggcat acacttcctc gacgaagccg
     1681 tcgatctcgc cgtcgagcag caggccgccg agggcctcgc cgaagtgcgc gtagaccagc
     1741 tgctcggtgt aggctggttc cgagaggatt gcctcgatgg cggggtcgta cacgtaggcg
     1801 ccgatgacgc cgagcctgaa tccctgctcc atcaacgccc gtggctcgtc ggcgggccac
     1861 gcaggaatct gcccggcacg gacatacagg ccgaactcct cacgtcttat cggtgctgaa
     1921 aacacggcga actcctgccg ggccgcagtg atggtcgcgc ccgtgagcag gtcgattcgt
     1981 ccctcgcgca gcttcgccag caacgccgcc cagctgtccc gttcgaatac cagcccgcag
     2041 cccgcgtcgg ccgccatggc gcgcagcagc tccacgtcga agccggccaa ctggccgccg
     2101 acgtcttcgt aatggaaggg tggccaggga tcccagccca tggtcaggcg gcaatcggca
     2161 tcgccgggta cctcgcgggg cgcctccgca gttactggcg tatccgctgc cgcggtcggt
     2221 tcggtgggcg gggcctggcg ggaacagccg acaccgagga acgcaaccgt gaaaaaaagg
     2281 aacggcagga acaggcggcg catgacccct ccttgggggc ccggcggctt gctgggggag
     2341 cagtcgtccg cgcgtggccc gtgctgcgag tataggccag gaatcgccgg gcgccgaccg
     2401 cagccggcgc tacttgctgc gcaggatctt gaggatttct tccagctcga cgcgcagctg
     2461 gcggacaatc tgcacgctct gcgtcaggtc ctccttggcg tcgtcgccgg tgagccgctg
     2521 gcgcgcgccg ccgatggtga agccctcctc gtacaggagg ctgcggatct ggcggatcaa
     2581 gagcacatcg cgccgctggt agtagcgccg gttgccgcgg cgcttgaccg gcttgagttg
     2641 cgggaattcc tgctcccagt agcgcagcac gtgcggcttc acgccgcaca gctcgctcac
     2701 ttcaccgatg gtgaagtagc gtttaccggg gatggccggc agctcgtcgt tattccccgg
     2761 ttccagcata ggcttccacg cgagtcttga gcttctggcc gggtcggaac gtcaccacgc
     2821 ggcgcgccgt gatggggatt tcctcgccgg tcttcggatt gcggcccgga cgccggttct
     2881 tgtcacgcag gtcgaaattg ccgaagcccg acaacttgac ctgctcaccg ttcgccagcg
     2941 cggtgcgcag ctcttcgaaa aacatttcga cgagctcgcg cgcttcacgc ttgttcaggc
     3001 ccatatcctg gaacagcgcc tcagccattt ctgccttcgt gagtgccatg tcagtctctc
     3061 atcctggcgt cgaactccga cgcgagacgg gccaccaccg cggccacgac ccggtcggcc
     3121 tcttcgtcgg taagagtgcg cgaagattcc tgtaatatca agccgaaagc cacacttttt
     3181 cgacccgaat ctatacgttc gccgctatac acatcgaaaa ccttggattc tgttaaaagg
     3241 gcgccgcccg cggaccggat cgcatcgacc attgcctgca ccggcacggc ctcgtcgatc
     3301 accacggcaa cgtcgcgacg aatggccggg aagcgcgaga tttcgcgaaa cgccggcaac
     3361 tccgccgcaa aacaagttgc ctcgtccagt tcccacagca acggcgcacg ctccaggtcc
     3421 aacgctctca ccaggcgcgg atgcagcgcg ccgatccagc cggcctcgcg accgtcccgc
     3481 aggatgcgcg cgcactgtcc cgggtgcagc gcggaatgct ccgccggcgc gaacacccat
     3541 tccgcgttgc tgagacccag cagcgcctcg acgtcgccct tggcgtcgtg gaagtccgcc
     3601 ggcaccgccg gggcgcccca ctgctccgga aggcgggcgc ctgcgatgag cccagcgata
     3661 gtagtaagtt gtttcaattc attgtcttgc gagataaatc tcaggccctg ctcgaaaata
     3721 cggacgcggc tctgccgccg gctgagatta cgcttgaggg tgtgaacgag cccgggccag
     3781 agcgagaccc gcatgtcgct cagctcagtc gaaatcgggt tgctgagcgg cagagcccgt
     3841 gattcgggaa acagcttcgt ctgcagctgc gggtcgacga agctgtaggt caccacttcc
     3901 tggtagccgc gcgccgccag catatcggcc aggcgcaccc cgggcacccg ggcttcactg
     3961 gcttcgccga gcacggcggc gcccgcgccg cgcgcctccg gcaggcggtc gtagccatgc
     4021 acccgggcaa cttcctcgat gagatccgcc tcgatggcga tgtcaaagcg atgcgagggt
     4081 ggcacggcct cccagccctc gtcgctggga gccaggcgaa ttccgagccc ctcgagaatc
     4141 cgctcgatct cctcatctgg caactcgtgg ccgagcagca tggcgagccg cgcacgccgc
     4201 aagcgcaccg ggctgcgctc gggcaggtat tgctcggcaa cgcgttcgat caccgggccc
     4261 ggttgcccgc cggcgagctc cgccagcagc gccgtggcgc gctccacgg
//
LOCUS       DAOMPG010000208         4255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8639_length_4255_cov_3.449286, whole genome shotgun sequence.
ACCESSION   DAOMPG010000208 DAOMPG010000000
VERSION     DAOMPG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_4255_cov_3.449286"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>4255
                     /locus_tag="PLS34_10385"
     CDS             160..>4255
                     /locus_tag="PLS34_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRP87910.1"
                     /translation="MGHGAAIRRSFSVALMLATGGLAMPALAQVAGYTPGEFSVDPAG
                     AATYQVPLSVPPGAAGMQPELALLYHSRAGNGQLGVGWSLSGLSAISRCPAIRDSEGV
                     ARSDGVTLSSADRFCLDGQLLRHQSGTYGANGVRYFTEVQSFQEVFSYGVPNGAPTRF
                     EVTDRAGLKRIYGQTTDSRITSVGDIAGVPIVWALNRIEDKLGNYITFSYGRDATLGE
                     YWPSEIKWFNRNSVVLGRVVLSYTTSRQDKSYGFGFGGTRQALTRRLSALQVYEGQSN
                     AVRRYGLDYQQAPYSGLSRLVSIQECAGTGAPCLPATTFAWENGQQGFIHSPVQQVGP
                     AGGNVKMLDVSGDGRLDLVQRTSNSIRIYFAKNGGSYVTSSVPAGSNMRFEEAVVLDY
                     NGDGLMDLLVANTSSGNWDLYRSTGTGFAYQNTGRPHYGAYSRNPAAFDVTGNGVPEL
                     FYKSGGKLWVYRGADGGGFLASPVNTGWTVADGQKFMPLEFDGDGLTDLLVTYDGCQP
                     SGGGGGGGGGGGGFEIESAPGGGGFLVETQSSGSTTCGMTSGPLRWNPDTDTLERPWA
                     DVGVRHHEQPMLLDINGDGLTDIVSIPPSGTQMQVSLNRGGRWVSEWTGLDSHQWTKA
                     VVLDWNLDGRDDLLVARPDGYFTALTYRNNILETVPTGLSASNAAYATADWNGNGLAD
                     LWHISGSNWRVSRHRGQRAGHLTRIQNGLGDELAISYAALSDGYGTGLSVYEGHVGSD
                     AYTTSPRVRNFLGPVFVVGSYAADAGIDSAGGGPQKVVTRYRYAGAKVNAHGRGFLGF
                     RRVRATNENTLIVTENIHFQEFPYTGMVKEARQRMPDTTSVQELPWEAWEDWFNENCA
                     YPYTEQCQGPPPPTVIIEPGPVISETVNTVAKKTLGAPETARVYFPHLESSTETSRDF
                     TSGGGSAVYRRTVTSYVYDNDGNPTQIVATVDNGSGGDVHTTTTVNQYTHTAACPSRL
                     DQSTVTQVVPSAGGHGPLARSRTASFDYHPTHCQLTVETSNAGTAAALTRTYTYDVFG
                     NRHTETITGAGISPARVTTTNFDANGRYLVGVVDAAGHAQSFSWHQGFGKRLSATDAN
                     GLTVSWLYDAFGREERVTGPRSTQFTETARAWCGSGGCQHPEAVLKVTRTGSGGATEQ
                     TSSVTELDRLGREVAAGERNVQGHMTYALTFFDPAGRPYANSGPYRPGVDSSACWTVR
                     HYDPLGRVRHEYAAADPSHCSGLAAPPLGAAPTGWSHTETTYNGLTTAVTDPEGRQRE
                     QIVNVMGRVRFAREHDGTTWRQTEYRYDGHGNTTWVQDAAGALTISEFDDAGRKTSMS
                     DPNMGNWSYAYNAAGELTAQTDANSVTVSMTYDVLGRIKTRSEPE"
BASE COUNT          769 a         1398 c         1450 g          638 t
ORIGIN      
        1 agtttccgtc tactacggac aaatgtcaga tcccacgccc cgaatccgcg agtagtcaat
       61 gtgctgcaca tcgcgcggcc cggggaatct tgacaaaaac accaaatatg gtcgtaatca
      121 cccaaaaaca gcacaaccgt gttgggggag ggtggggcga tgggccacgg tgcggcgatc
      181 cggcgttctt tctcggtggc attgatgctc gcgacgggag ggctggcgat gccggccctg
      241 gcccaggtgg ccggctacac gccgggggaa ttctccgtag acccggcagg cgctgcgacc
      301 taccaggtcc cgctgagcgt gccgccgggc gccgccggca tgcagcccga actggcgctg
      361 ctgtaccact cgcgcgccgg caacggccag ctcggcgtgg gctggtcgct atcgggcctg
      421 tcggcgatct cccgctgccc ggcgatcagg gactccgagg gcgttgcgcg cagtgatggc
      481 gtcaccctgt cgtccgccga ccgcttctgc ctggacggcc agctgctccg gcaccagtcg
      541 gggacctacg gcgccaacgg tgtgcgctac ttcaccgaag tccagagttt ccaggaagtc
      601 ttcagctatg gcgtccccaa tggtgcgccg acccgtttcg aggtgaccga ccgcgccggc
      661 ctgaagcgca tctacggtca gacgacggac agccggatca cctcggtggg ggacatcgcc
      721 ggcgtgccga tcgtctgggc gctgaatcgc atcgaggaca agcttggcaa ttacatcacg
      781 ttcagctacg gacgggacgc gacgctcggc gagtactggc cgagcgagat caagtggttc
      841 aaccgcaaca gcgtggtgct gggtcgggtc gtgctgagtt acacgacgag ccggcaggac
      901 aagtcgtacg gcttcggctt cggcggtacc aggcaggcgc tgacccggcg gctcagcgcg
      961 ctgcaggtct acgaaggcca gagcaacgcg gtgcggcgct acgggctcga ctaccagcag
     1021 gcgccgtaca gtggcctgag tcgactggtc tcgatccagg aatgcgcggg caccggtgcg
     1081 ccgtgcctgc cggcgacgac tttcgcgtgg gagaacggtc agcagggctt catccactcc
     1141 ccggtccagc aggtgggtcc ggcgggcggc aacgtcaaaa tgctggacgt gagcggtgac
     1201 ggccgcctcg acctggtgca gcggaccagc aactccatcc gaatctattt cgcgaagaat
     1261 ggcggcagct acgtcaccag ttcggtgccg gccggctcga acatgcgatt cgaggaggcg
     1321 gtcgtgttgg actacaacgg cgacggcctg atggacctgc tggtggccaa caccagcagc
     1381 ggcaactggg acctctatcg ttccacgggc accggctttg cctaccagaa caccggccgg
     1441 ccgcactatg gcgcatactc gaggaacccg gcggcgttcg acgtcacggg caacggcgtg
     1501 ccggagctgt tctacaagag cggcggcaag ctctgggtct accgcggcgc ggacggcgga
     1561 ggctttctcg cgagcccggt gaataccggc tggacggtcg ccgacggcca gaaattcatg
     1621 ccgctggaat tcgacggcga cggcctgaca gatctgctcg tcacctatga cggctgccag
     1681 ccgtcgggcg gtggtggtgg cggcggtggt ggcggtggcg gattcgagat cgagtcggcc
     1741 ccaggcggcg gcgggttcct cgttgaaaca caaagcagcg gctcgaccac ctgcggcatg
     1801 acgtcgggcc cgttgcgctg gaacccggac accgacacgc tggagcgccc ctgggcggat
     1861 gtcggtgtgc ggcaccacga gcagccgatg ctgctggata tcaatggtga cggcctgacc
     1921 gacatcgtgt ccatcccgcc ctccggcacg cagatgcagg tatcgctgaa ccggggtggt
     1981 cgctgggtga gcgaatggac cggcctggac agtcaccagt ggaccaaggc ggtcgtgctg
     2041 gactggaacc tggacggtcg cgatgacctg ctcgtggcgc gtcccgacgg gtacttcacc
     2101 gcgctgacct atcgcaacaa tattctggag accgttccca ccggcctcag tgcgagcaac
     2161 gcggcctacg ccacggcgga ctggaacggc aatggcctgg ccgacctctg gcacatttcg
     2221 ggcagcaact ggcgcgtcag tcgccaccgg ggccaaaggg ccggacacct gacgcggatt
     2281 cagaacgggc ttggcgacga gctggcgatc agctacgcgg cgctctcgga cggctacggt
     2341 accgggctga gcgtgtacga agggcatgtg ggcagcgacg cctacacgac gtcgccgcgg
     2401 gtgcggaatt ttctcgggcc ggtcttcgtg gtgggaagct atgcggcgga tgccggcatc
     2461 gacagtgccg gtggagggcc gcagaaggtc gtgacgcgct atcgctacgc cggcgccaag
     2521 gtgaacgcac atggccgcgg ttttctcggc ttccggcggg ttcgcgccac caacgagaac
     2581 acgctcattg tgaccgaaaa tattcacttc caggagttcc cgtacaccgg catggtgaag
     2641 gaagcgcgcc agcggatgcc cgacacgacc tccgtgcagg aactgccctg ggaggcctgg
     2701 gaggactggt tcaacgagaa ctgcgcttat ccgtacaccg aacagtgcca ggggccgccg
     2761 ccgccgaccg taatcatcga accgggtccc gtcatcagcg aaaccgtgaa cacggtggcg
     2821 aagaagaccc tgggcgcccc cgaaacggca cgggtatatt tcccgcacct cgaatcgagc
     2881 acggagacga gtcgcgactt cacctcaggc gggggcagcg ccgtctaccg ccggaccgtg
     2941 accagttatg tctacgacaa cgacggcaat cccacccaga tcgtggcgac ggtcgacaac
     3001 ggttcgggcg gggacgtgca caccaccacg accgtcaacc agtacacgca cacggcggcc
     3061 tgtccctccc ggctggatca gagtacggtg acgcaggtcg tcccttccgc cggtggccat
     3121 ggcccgctcg cccgcagccg cacggcaagc ttcgattatc atccgacaca ctgtcagttg
     3181 accgtcgaga ccagcaacgc cggcacggca gcggccctga ccagaaccta cacctacgac
     3241 gttttcggca atcggcacac cgagacgatc accggcgcgg ggatttctcc cgcgcgcgtc
     3301 acgaccacga acttcgatgc gaacggccgc tacctcgtcg gggtggtgga tgccgcggga
     3361 cacgcgcaga gcttcagctg gcaccagggt ttcggcaagc gcctgagcgc caccgacgcc
     3421 aacggcctga cggtctcgtg gctgtacgac gcgttcgggc gcgaggagcg cgtgaccggc
     3481 ccgcgcagca cacagttcac cgagaccgcg cgggcgtggt gcggcagcgg cgggtgccag
     3541 caccccgagg ccgtgctgaa ggtgacgcgc accggcagcg gcggcgccac cgaacagacc
     3601 tcgagcgtca cggaactcga ccggctcggc cgtgaggttg ccgccggcga acgcaacgtc
     3661 cagggccaca tgacctatgc cctgacgttt ttcgatcctg cgggccggcc ctatgcgaac
     3721 tcggggccgt atcggcccgg cgtggacagc agcgcctgct ggacggtgcg ccattacgac
     3781 ccgctcgggc gggttcgcca cgagtacgcg gcggcggatc cgtcgcattg cagcggtctg
     3841 gcggccccgc cgctgggcgc ggcgccgacc ggctggtcgc acaccgagac gacctacaac
     3901 ggcctgacga ccgccgtgac cgacccggag ggccgccagc gcgagcagat cgtcaacgtc
     3961 atgggccggg tgcgcttcgc gcgcgagcac gacggcacga cctggcgcca gaccgagtac
     4021 cgctatgacg ggcacggcaa cacgacctgg gtgcaggacg cggccggggc tttgaccatc
     4081 tcggagttcg atgacgccgg ccgcaagacc tcgatgtccg acccgaacat gggcaactgg
     4141 agctacgcct acaacgccgc cggcgagctg accgcgcaga ccgacgccaa cagcgtcaca
     4201 gtcagcatga cgtatgacgt gctcgggcgg atcaagacgc gcagcgagcc ggaag
//
LOCUS       DAOMPG010000209         4241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8685_length_4241_cov_5.208791, whole genome shotgun sequence.
ACCESSION   DAOMPG010000209 DAOMPG010000000
VERSION     DAOMPG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_4241_cov_5.208791"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLS34_10390"
     CDS             complement(<1..678)
                     /locus_tag="PLS34_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87911.1"
                     /translation="MIKRYLPLLFVLLVVIATVVVFLAYRDPEPTPSAPADGQPADAE
                     DRGGATGADGVREFRVESEEEYQRTLEALGTGNDEIEAWARSRGFPPATYTSSAGMPL
                     ERNYNREKDGRLLELAEGGDRWAMQVLAQRIGPEMPLEAIDWYRRAVLHGSAYAAYRL
                     GNFYHDVARWIAVNHDDRDEVLEIARREDPLAYSALGWLLVAEYEAGLPPGAMSATLA
                     GFQAPAEG"
     gene            complement(686..1576)
                     /gene="cyoE"
                     /locus_tag="PLS34_10395"
     CDS             complement(686..1576)
                     /gene="cyoE"
                     /locus_tag="PLS34_10395"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRP87912.1"
                     /translation="MPGTGLGARLGDYFELCKPKVVGLIVFTAVVGMFLAVPGLVPLD
                     KLLFGTLGIGLAASSAAAINHVLDARIDAVMRRTRRRPLPTGHLREIEALTFAVLLGL
                     VSMLILWLLVNPLTAGLTFFSLIGYAIVYTVFLKRATPQNIVIGGAAGAAPPVLGWVA
                     ITGEVHAHALLLFLIVFTWTPPHFWALAIARKDEYAEVNIPMMPVTHGIEFTRLQILL
                     YTILLCLVSLLPWLTGMSGLLYLAGAIGFGGGFLYYAIAMMRSDRPELPMKTFAYSIT
                     YLMGIFSFLLADHYVPLVLG"
     gene            complement(1739..2326)
                     /locus_tag="PLS34_10400"
     CDS             complement(1739..2326)
                     /locus_tag="PLS34_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase assembly protein"
                     /protein_id="HRP87913.1"
                     /translation="MSQQGKPKRGNLQAWGLFLLFFGPLAVAVYLYYDTGWRPGHTSN
                     HGELIVPAVPLPEVELPLPGGGRTDADFLRHHWSLVYVGRDGCAERCRDALYNGRQMR
                     LALGRLMDRVERVYLYVGEPPAAGFVAAEHPDLLVADATGPEALPLLEALAGPAEGYW
                     LIDPLGNAMMRYPADQAPRGMLEDLKRLLRLSRIG"
     gene            complement(2329..3039)
                     /locus_tag="PLS34_10405"
     CDS             complement(2329..3039)
                     /locus_tag="PLS34_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRP87914.1"
                     /translation="MAAARRRFRPGVPATIFAGFFLPLLLALGTWQVDRAGEKQALFD
                     DFAAGGEAVALEANSRGLEELRRYAPVRATGRYLADRQFLLDNMVAGGQAGYRVITPL
                     LIAEGHAVLVDRGWLPRDFSTPVPPPIEVDEQPRTVAGRLDQLPRPGIALDTPAEPGW
                     PKVVQFPTLDELSGWLELELVPGLLLLDAAAPDGYRRDWRPSDFGPERHIGYAFQWFA
                     LAATLVILYLAWSFRNSD"
     gene            3117..3314
                     /locus_tag="PLS34_10410"
     CDS             3117..3314
                     /locus_tag="PLS34_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRP87915.1"
                     /translation="MKFLILVAFVAILASLASGLVYLFKDDHDSRRVVKALTWRIGLS
                     VAMFVVLLLAYAMGWIQPGQL"
     gene            complement(3416..>4241)
                     /locus_tag="PLS34_10415"
     CDS             complement(3416..>4241)
                     /locus_tag="PLS34_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRP87916.1"
                     /translation="YWPIVGAVGLFFMMLGLSSWLNGVGLMGWVAWLGVAIVLVMICG
                     WFATVINESEGGLYSGQVDRSFRMGMTWFIISEVMFFAAFFGALFYARQLAIPWLGGE
                     GNNFFTHLLLFPEFQAAWPTGGPEAWGGDFEIMGAGGLPALNTAILLTSGVTITIAHH
                     AIKDGNRSLTAWGLFGTIALGLLFLSLQAYEYVHAYQDLNLRLTSGIYGSTFFMLTGF
                     HGLHVTLGAIMLIVIWVRVLKGHFTPKQHFGFEGVAWYWHFVDVVWLGLYIFVYWL"
BASE COUNT          751 a         1423 c         1445 g          622 t
ORIGIN      
        1 gccctcggcg ggcgcctgga atccggccag ggtcgcggac atggcccccg gcggcaggcc
       61 ggcctcgtac tccgccacca gcaaccagcc gagcgcactg taggccagcg ggtcctcgcg
      121 ccgggcgatt tcgagcactt catcgcggtc gtcgtggttg acggcgatcc agcgagcgac
      181 atcgtggtag aaattgccga gcctgtaagc cgcgtacgcg gagccgtgca gcaccgccct
      241 gcggtaccag tcgatggctt cgagcggcat ctccgggccg atccgttgtg ccagcacttg
      301 catcgcccag cggtctccgc cttccgccag ttccagcagc cggccgtcct tttcgcgatt
      361 gtagttgcgc tccagcggca tcccggcgga gctcgtgtag gtggccggag gaaacccgcg
      421 gctgcgcgcc caggcctcga tctcgtcgtt gccggtgccc agcgcctcga gcgtccgctg
      481 gtactcctcc tcggactcga cccggaattc ccgcaccccg tcggcgccgg tcgcgccgcc
      541 ccggtcttcc gcgtccgccg gctggccgtc ggccggggcg gacggcgtcg gctccggatc
      601 gcgatatgcg aggaatacga ccacggtggc gatgaccacc agcagcacga acagcagcgg
      661 caggtagcgc ttgatcatgc cggcctcagc cgagtaccag cggcacgtag tggtcggcga
      721 gcaggaacga gaagatgccc atgagatagg tgatcgagta agcgaaggtc ttcatgggga
      781 gctccgggcg gtcggagcgc atcatggcga tggcgtagta caggaaaccg ccgccgaagc
      841 cgatcgcacc ggcgaggtac agtaagccgc tcatgccggt gagccagggc agcaggctca
      901 ccaggcacaa caggatcgtg tacagcagga tctgcaggcg cgtgaactcg atgccgtgcg
      961 tcaccggcat catcgggatg ttcacttcgg cgtattcgtc cttgcgtgcg attgccagcg
     1021 cccagaaatg cggcggcgtc caggtgaaca caatcaggaa cagcagcaat gcgtgggcat
     1081 gcacctcgcc ggtgatggcc acccagccga gcaccggcgg cgcggcgccg gcggcgccgc
     1141 cgatgacgat gttctgcggt gtggcgcgct tgaggaacac ggtatagacg atggcgtagc
     1201 cgatcaggct gaagaacgtc agcccggcgg tgagcggatt gaccagcagc cagaggatga
     1261 gcatcgagac cagccccagc agcaccgcga acgtcagcgc ctcgatttcc ctgaggtggc
     1321 cggtcggcag cgggcggcga cgggtgcgcc gcatgacggc gtcgatgcgc gcatcgagca
     1381 cgtggttgat cgccgcggcc gaggaggccg ccaggccgat tccgagcgtg ccgaacagca
     1441 gcttgtcgag gggcaccagg ccgggcaccg ccaggaacat gccgactacg gccgtgaaca
     1501 cgatcagccc gaccaccttc ggcttgcaca gctcgaaata gtcgccgagg cgggcgccga
     1561 ggcccgttcc gggcagcttt cgagcttcag tttgcacgcc aggacacctt gtggaatctg
     1621 ccttcgggcg gcgccggcgg cgccgcgagt cctttgcgcg ccgcaatccg cgacgccggt
     1681 ggaattgtgg aggatgcggc ccgcgcagtc gaccccgagg cgatgtaacg gacacccatc
     1741 agccgattct cgacagccgg agcaggcgct tgaggtcctc cagcatgccg cgcggcgcct
     1801 ggtccgccgg atagcgcatc atggcgttgc caagcgggtc gatcagccag tagccctcgg
     1861 ccggaccagc cagcgcctcg agcagcggca gggcctcggg ccccgtcgcg tcggcgacca
     1921 gcaggtcggg gtgctcggcg gcgacgaagc ctgcagccgg cggctcgccg acgtacaggt
     1981 agacgcgttc cacgcgatcc atcagccggc caagcgcaag ccgcatttgc cggccgttgt
     2041 acagcgcgtc gcggcagcgc tcggcgcaac cgtcgcggcc gacatagacc agtgaccagt
     2101 gatgcctcag gaaatccgca tcggtgcggc cgccgcccgg caggggcaac tcgacctcgg
     2161 gcagcggcac ggccggtacg atcaactcac catggttgct ggtgtgtccg gggcgccagc
     2221 cggtgtcgta gtagagatac acggcgaccg ccagcgggcc gaagaacagc aggaacaggc
     2281 cccatgcctg caggttgccg cgttttggtt tgccttgctg tgacatgttc agtccgagtt
     2341 cctgaaagac caggcgaggt agaggattac cagggtcgcg gcgagcgcaa accactggaa
     2401 ggcataaccg atgtggcgct ccgggccgaa atccgacggc cgccagtcgc gccggtaacc
     2461 gtccggcgcg gccgcgtcga gcagcagcaa cccgggtacg agctccagct ccagccagcc
     2521 cgacagttcg tccagcgtgg ggaactgcac gaccttcggc cagccgggct cggccggcgt
     2581 gtccagcgcg atgcccgggc gcggcagctg gtcgagtcgc ccggccacgg tgcgcggctg
     2641 ctcgtccact tcgatgggcg gcggcacagg cgtcgagaaa tcgcgcggca gccagccgcg
     2701 gtcgaccagc accgcgtggc cctcggcgat cagcaacggc gtgatcaccc ggtagccggc
     2761 ctggccgccc gccaccatgt tgtcgagcag gaattgccgg tcggcgagat agcggcccgt
     2821 cgcgcgcacc ggcgcgtagc ggcgcaactc ttccaggccg cgggagtttg cctcgagcgc
     2881 aacggcttcg ccgccggccg cgaaatcgtc gaacagtgcc tgcttctcgc cggcccggtc
     2941 gacctgccag gtcccgagcg ccagcagcag gggcaggaaa aagcccgcga agatggtcgc
     3001 gggtaccccg ggccggaaac ggcgtcgcgc ggcggccatt tcaggcctcc gggacgcggc
     3061 gacacgcgtc tcggcagcta tactggccat cctgtccgga gtccttcatg agcgcaatca
     3121 agttcctgat actggtcgcc ttcgtcgcca ttctcgcgag cctcgcatcg gggctcgtct
     3181 acctgttcaa ggacgaccac gattcccgcc gggtggtcaa ggcgctgacc tggcgcatcg
     3241 gcctgtcggt ggccatgttc gtggtgctgc tgctcgccta cgcgatggga tggatccagc
     3301 ccggccagct gtaaggcgaa ccgctccgcc agggccggga atcgtctatt ccttgacgag
     3361 acggggcgcc gctgtcgcca gggcgccccg tgcccgtgtc gctgaaacct gccggctaaa
     3421 gccagtaaac gaagatatac aggcccagcc agaccacgtc cacgaaatgc cagtaccagg
     3481 ccacgccctc gaagccgaag tgctgcttcg gcgtgaaatg ccccttcagc acgcgcaccc
     3541 agatcacgat cagcatgatg gcgccgagcg tgacgtgcag gccgtggaag ccggtcagca
     3601 tgaagaaggt gctgccgtaa atgcccgagg tgagcctcag gttcaggtcc tggtaggcat
     3661 gcacatactc gtaggcctgc aggctgagaa acagcaggcc gagcgcgatg gtgccgaaca
     3721 ggccccaggc cgtgagcgag cggttgccgt ccttgatggc gtggtgggcg atcgtgatgg
     3781 tgacgccgct ggtcagcagg atggcggtat tgagcgccgg caggccgccc gcacccatga
     3841 tctcgaagtc gccgccccag gcttcggggc cgccggtcgg ccaggccgcc tggaactcgg
     3901 ggaatagcag caggtgggtg aagaaattgt tgccctcgcc accgagccag ggaatcgcca
     3961 gctgccgcgc gtagaacagc gcgccgaaga aggccgcgaa gaacatcact tccgagatga
     4021 tgaaccaggt catgcccatc ctgaaggagc ggtccacctg gccgctgtac aggccgcctt
     4081 cgctctcgtt gatgaccgtc gcgaaccagc cgcagatcat caccagcacg atcgccacgc
     4141 cgagccatgc gacccagccc atcaggccga cgccgttcag ccaggacgac aggccgagca
     4201 tcatgaagaa caggccgacc gcgcccacga tgggccagta g
//
LOCUS       DAOMPG010000210         4187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8819_length_4187_cov_4.645208, whole genome shotgun sequence.
ACCESSION   DAOMPG010000210 DAOMPG010000000
VERSION     DAOMPG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_4187_cov_4.645208"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLS34_10420"
     CDS             <1..589
                     /locus_tag="PLS34_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRP87917.1"
                     /translation="AVAGLEAARKARAGTVTQLDADLARRGAEADRLREEAAGLERLL
                     EQLRAAVVDLPVPDATPFADQRGRLDWPVAGRLARDFGERRGEGPRSNGVLIAAERGA
                     DVQAVWHGRVAYADWLPGLGLLLVLEHGDGFMSLYGHNEVLFSAVGDWVAAGQIVGQV
                     GDSGGRDNAALYFEIRSGARPENPAPWFRERLKRR"
     gene            759..1976
                     /locus_tag="PLS34_10425"
     CDS             759..1976
                     /locus_tag="PLS34_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP87918.1"
                     /translation="MKSLLLRGLLAVWLGVVLGLSVSLAPAVLADRQPEADTLPWEEA
                     RLLAEVIERVKRDYVDRVDDHDLIEAAIRGMIADLDPHSAFLDPGEFQDIRVSTSGRY
                     TGIGVEVGAQDGSIVVIAPIDGSPAARAGIRAGDVIFSVDGMPVSGADLEDTVTRMRG
                     EAGTTVVLGITRPDQAEPITVELRRGPVAVRSVRAETLPGGEPYVRISHFTDATGEDL
                     ERALDELRRNAGGRLSGLVLDLRDNPGGVLNAAVAVADVFLDSGLIVSAEGRMADARF
                     RLEATPGDALDGAPLSVLVNGGSASASEIVAGALKDHGRATVVGKPTFGKGSVQTIMP
                     LSEGRAIKLTTSRYFTPSGGSIHARGVTPDVLVEAGAAGAEDVQLASAVAALYGRRIA
                     LHAEAAAAGARGE"
     gene            1982..2557
                     /locus_tag="PLS34_10430"
     CDS             1982..2557
                     /locus_tag="PLS34_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRP87919.1"
                     /translation="MSRVLVPLAEGFEEIEAVTVIDLLRRADIEVVTAGLAPGPVTGS
                     HGVCLLPDMDLDAALEQHFDMVALPGGMPGAANLGADDRVLALLRLLADSGRFTAAIC
                     AAPGVLAKAGLLAGKRATSYPGFLGPENAPGTLLLEDAVVEDGKVITSRGPGTAMDFA
                     LALIQRLEGAAVRRDVESRLQRPVPRPPTEG"
     gene            2564..3658
                     /locus_tag="PLS34_10435"
     CDS             2564..3658
                     /locus_tag="PLS34_10435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP87920.1"
                     /translation="MLPLFKALSRDLDEDIDRAELRGLARNAADGAWLLLVLAALYVL
                     TPGGQMLRPTLAIGAMGAYAVLTLGLRFLPRFHRETRLKIAAELLAMVVFTTVFLFSV
                     ATHASLLLVLYLLPVIISALTLGRWPTLVVTVLSVAGFLLAALLRDPAGLPTGRQIVE
                     LGIALAPFLLVAYVAALLAHEVHMAKQRIRTLSETDELTGLANLRAFSRLHRQEHERA
                     VRHHRVYSIIVMDLDGLKQINDNFGHDTGDRAIVVLANVIARLIRTTDAAARFGGDEF
                     VMLLSETDAEQTLRVAHRIRSAVERCTIEVGGRMVHLSVSVGTASFPSDTDDLREIIA
                     AADKAMYRDKQARLRLPAEPETRTKRLEVV"
     gene            3698..4150
                     /locus_tag="PLS34_10440"
     CDS             3698..4150
                     /locus_tag="PLS34_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HRP87921.1"
                     /translation="MTGVLTLAWFHYLAMMFLAAALIAEHLMFAPRPTLPVARKIVVV
                     DAIYGISLVVVFLTGVARMFHGGKGALFYMQNGAYHTKFTLFIVMAVLWLYPALKFFA
                     WRRALRRGETPAMDDSEAKRVLMAIRLQLLILVFLPLLAAMMARGVWS"
BASE COUNT          564 a         1449 c         1497 g          677 t
ORIGIN      
        1 ggcggtggcc gggctggagg cggcacgcaa ggcgcgtgcc ggcaccgtca cgcagctcga
       61 tgcggacctc gcgcgacgcg gtgcggaggc cgatcgactg cgcgaggagg ccgccggcct
      121 cgagcgcctg ctcgagcagt tgcgcgccgc ggtggtggac ctgccggtgc cggacgcgac
      181 gcccttcgcc gaccagcgcg gccggctgga ctggccggtc gcgggccggc tggcgcgcga
      241 cttcggcgag cgccgtggcg agggaccgag gtccaacggc gtgctgatcg ccgccgagcg
      301 gggcgcggac gtacaggcgg tgtggcatgg ccgggtggcc tacgccgact ggctgccggg
      361 gctcggcctg ttgctggtgc tggagcacgg cgacggcttc atgagtctct atggccacaa
      421 cgaggtcctg ttcagcgcgg tcggcgactg ggtcgcggcc gggcagatcg tcggccaggt
      481 gggcgacagt ggcggccgcg acaacgccgc gctgtacttc gaaatccgta gcggcgctcg
      541 gcccgagaac ccggcaccct ggtttcgtga gcggctgaaa cgccgttaaa acagagagtt
      601 atcgacatag tgtatgcaaa ataattaatc cagccgggcg gctcgggcgg tttgggccag
      661 cgcgggccgg catgcccggc ggggcgaccg cgcgattcac cgccgccgcg ccggaattct
      721 gttattttca gcggctggaa ccttgcccgg gaccttccat gaagtccttg ctgttgcgcg
      781 gtctgcttgc cgtctggctc ggcgtggtgc tcggcctgtc ggtctcgctc gccccggccg
      841 tgctggcgga tcgtcagccg gaggccgaca cgctgccgtg ggaagaggcc cggctgctcg
      901 ccgaggtcat cgagcgcgtc aagcgcgact acgtggaccg ggtggatgac catgacctca
      961 tcgaagccgc cattcgcggc atgatcgccg acctggaccc ccactcggcg ttcctcgacc
     1021 cgggggagtt ccaggacatt cgcgtcagca ccagcggccg ctacaccggc attggcgtcg
     1081 aggtcggcgc ccaggacggc agcatcgtcg tcatcgcccc gatcgacggc agccccgcgg
     1141 cgcgggcggg aatccgtgcc ggcgacgtga tcttctccgt cgacggcatg ccggtgagcg
     1201 gcgccgacct ggaggatacg gtcacccgga tgcgcggcga ggccggcacg acggtggtgc
     1261 tcggcatcac ccgtcccgac caggccgagc cgatcaccgt ggagctgcgc cgcggcccgg
     1321 tggcggtgcg cagcgtgcgc gccgagacgc tgcccggcgg cgagccctat gtgcggatca
     1381 gccacttcac cgacgctacc ggcgaggacc tggagcgtgc gctcgacgag ctccggcgca
     1441 acgccggcgg ccggctgtcc ggcctggtgc tcgacctgcg ggacaatccg ggcggcgtgc
     1501 tgaacgccgc cgtggcagtt gccgacgtct tcctcgacag cgggctgatc gtctctgcgg
     1561 agggccggat ggcggacgcc aggttccggc tcgaggccac ccccggcgat gcgctggacg
     1621 gcgcgccgct gagcgtgctg gtcaacggcg ggtccgcgtc ggcgtcggag atcgtcgccg
     1681 gtgccctgaa ggaccacggc cgggccaccg tcgtcggcaa gccgacgttc ggcaagggct
     1741 cggtgcagac catcatgccg ctgtccgagg gccgcgccat caagctgacc acctcgcggt
     1801 acttcacgcc gtcgggcggc tcgatccacg cccgcggcgt gacgcccgac gtgctcgtcg
     1861 aggctggcgc ggccggcgca gaggacgtcc agctcgcctc cgccgtcgcc gccctttacg
     1921 ggcgccgcat tgcgctgcat gccgaagcgg ccgctgccgg ggcgcgcggc gagtagctcc
     1981 cgtgtcgcgc gtgctcgttc ctttggctga gggtttcgag gagatcgagg ccgtcaccgt
     2041 catcgacctg ctgcgccgcg ccgacatcga ggtcgtcacc gccggcctgg cgccgggccc
     2101 ggtcaccggc tcacacggcg tctgcctgct gcccgacatg gacctcgacg ctgcgctcga
     2161 acagcatttc gacatggtcg cgctgccggg gggcatgccc ggagcggcca atctcggcgc
     2221 cgacgaccgc gtgctcgcgc tgctgcgcct gctggcggac agcggccgct tcacggccgc
     2281 gatctgtgcg gcgccaggcg tactcgccaa ggccgggctg ctggccggca agcgcgccac
     2341 cagctatccg ggcttcctcg ggccggagaa cgccccgggg acgttgctgc tggaggatgc
     2401 cgtggtcgag gacggcaagg tgatcacctc gcgcggtccc ggcacggcca tggatttcgc
     2461 actcgcgctg atccagcgcc tcgagggtgc agcggtccgt cgggacgtcg aatcccgcct
     2521 gcaacgcccc gtcccgcgcc cgccgaccga gggctgaggc cgcgtgctgc ccctctttaa
     2581 agcgctgtcg cgcgacctgg acgaggacat cgaccgggcg gagttgcggg gcctggcccg
     2641 caatgccgcg gacggcgcct ggctgctgct cgtgctggca gcgctgtacg tgttgacgcc
     2701 cggcgggcag atgttgcgtc cgacgctggc gatcggcgcg atgggcgcct acgccgtgct
     2761 gacgctcggg ttgcggttcc tgccgcgatt tcaccgcgag acccgcctca agatcgcggc
     2821 cgagctgctc gccatggtgg tattcacgac cgtgttcctg ttctccgtgg ctacccacgc
     2881 aagcctgctg ctggtcctct acctgttgcc ggtcatcatt tccgccctga cgctcgggcg
     2941 ctggccgacg ctggtcgtga ccgtgctgag cgtggccggc ttcctgctgg cggcgctgct
     3001 gcgtgatccg gcggggctgc cgaccgggcg gcagatcgtc gaactcggta tcgccctggc
     3061 gcccttcctg ctggtggcgt acgtcgcggc gctgctggcg cacgaggtac atatggcgaa
     3121 gcagcgtatc cgcaccctgt cggagaccga cgagctgacc gggctggcga acctgcgcgc
     3181 cttctcacgg ctgcatcgcc aggagcacga gcgtgcggtc cggcaccacc gggtctattc
     3241 catcatcgtc atggacctgg acggcctgaa gcagatcaac gacaacttcg gccacgatac
     3301 cggcgatcgc gccatcgtcg tgctggcgaa cgtcatcgcc cgcctgatcc gcaccaccga
     3361 cgcggcggcg cgcttcggcg gcgacgagtt cgtcatgctg ctgtcggaga ccgatgctga
     3421 gcagacgctg cgggtcgcgc accgcatccg ctccgcagtg gagcgctgca ccatcgaggt
     3481 cggcggccgc atggtccacc tttcggtcag cgtcggcacc gccagcttcc cgagcgacac
     3541 ggacgacctg cgggagatca tcgcggcggc cgacaaggcc atgtaccgtg acaagcaggc
     3601 gcggctccgc ctgccggccg agcccgaaac ccgcaccaag agactcgaag tcgtttaggc
     3661 tgtcgggcag agcaacggat cattcaggag atcgccgatg acaggggtgt tgacgcttgc
     3721 ctggttccat tacctcgcca tgatgttcct ggcggccgcg ctgatcgcgg aacacttgat
     3781 gttcgccccc cggccgacgc tgccggtggc gcgcaagatc gtcgtggtcg acgccatcta
     3841 cggcatttcc ctggtggtgg tgttcctgac cggcgttgcg cgcatgttcc acggcggcaa
     3901 gggcgcgttg ttctacatgc agaacggcgc ctaccacacc aagttcacgc tgttcatcgt
     3961 catggcggtg ctgtggctgt atcccgccct caagttcttc gcctggcgtc gggccctgcg
     4021 gcgcggcgag acgccggcga tggacgacag cgaagcgaag cgcgtgctga tggccatccg
     4081 cctgcagctg ctgatcctgg tgttcctgcc cctgctggcc gcgatgatgg cgcgcggcgt
     4141 ctggtcctga cgcgggcgaa caaggtacag gtcctgcacc tgctggc
//
LOCUS       DAOMPG010000211         4185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8829_length_4185_cov_4.765860, whole genome shotgun sequence.
ACCESSION   DAOMPG010000211 DAOMPG010000000
VERSION     DAOMPG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_4185_cov_4.765860"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLS34_10445"
     CDS             <1..182
                     /locus_tag="PLS34_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-phosphate glycerol 3-phosphate
                     acyltransferase"
                     /protein_id="HRP87922.1"
                     /translation="FAAAMAPVWLAATGQSGRPLFWFALLMAGFVAWTHRSNIARMRA
                     GNENRMFRGLVGRRR"
     assembly_gap    342..351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <352..>964
                     /locus_tag="PLS34_10450"
     CDS             <352..>964
                     /locus_tag="PLS34_10450"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRP87923.1"
                     /translation="PPLVPLDADAVRRALPTPVARRIGALEVLAETESTNARVLAAER
                     KVGELVVCLAEYQSAGRGRRGRRWLAPPGSGICLSVGGRLPAAPSDYAALPTAVGVAC
                     AAALEALGAEAIGLKWPNDLLLGGHKLGGILIELRGESQGPAVVAVGLGLNVRLGPAT
                     RESIAAAGGLPAADLAALAASAPVDRNRLAAALAAAIAGCLEHA"
     assembly_gap    965..1010
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1159..1920
                     /locus_tag="PLS34_10455"
     CDS             1159..1920
                     /locus_tag="PLS34_10455"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRP87924.1"
                     /translation="MKLLLDLGNTRLKAALGMPDGRLVPLGEAGHRAAGMAVALDHAL
                     GDTALAGVTGALCANVAGAPAGDALATALGGRGVAVEFLRPGREAGGVRCAYAEPARL
                     GADRWAALLGARGLEDGACLVVDAGSALTIDALSPGGQHLGGWILPGLAMMVAALEAR
                     TGDLGALRRASQASPPADFPADTAPAMEGGALLAACGAVRLARKRLECRCRVPARLLL
                     AGGDAARLVTELDGAELQPDLVLRGLARLAADKAC"
     gene            2042..2320
                     /locus_tag="PLS34_10460"
     CDS             2042..2320
                     /locus_tag="PLS34_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87925.1"
                     /translation="MKKLTIALLALPLVALSGAALAGDAEAGKAKAESCMDCHAGEDF
                     EGMSLEELIAANKAVIAGEQKHPPGLEDVTVEDAEDLAAYFYAEAGGE"
     gene            2427..2502
                     /locus_tag="PLS34_10465"
     tRNA            2427..2502
                     /locus_tag="PLS34_10465"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2460..2462,aa:Thr,seq:tgt)
     gene            2586..2864
                     /locus_tag="PLS34_10470"
     CDS             2586..2864
                     /locus_tag="PLS34_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87926.1"
                     /translation="MKKLTTIMMALPLALFSAGALAGDAAAGAAKAEACMDCHAGEDF
                     EGMSAAEIADAIRAVLAGQAKHPPGVDDITEADADDIAAYFYAEAGGE"
     gene            2985..3260
                     /locus_tag="PLS34_10475"
     CDS             2985..3260
                     /locus_tag="PLS34_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP87927.1"
                     /translation="MNKLTPALLALSLATFSGAALAGDAAAGEAKAALCLDCHYADDF
                     PNVSAEEVETMIRAARAGEVKHGEEINELAEEDIADVAAYFAHEGSK"
     gene            3271..3549
                     /locus_tag="PLS34_10480"
     CDS             3271..3549
                     /locus_tag="PLS34_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87928.1"
                     /translation="MNKLVILLAALPLTALAGTALAGDPAAGEAKADACLECHEPDYF
                     VGEDADAIAAMIREVRAGEIKHKDDAPRDLPEEDIADVAAYFAHEGAK"
     gene            3676..3760
                     /locus_tag="PLS34_10485"
     tRNA            3676..3760
                     /locus_tag="PLS34_10485"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3710..3712,aa:Tyr,seq:gta)
     gene            3840..3913
                     /locus_tag="PLS34_10490"
     tRNA            3840..3913
                     /locus_tag="PLS34_10490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3872..3874,aa:Gly,seq:tcc)
     gene            3960..4035
                     /locus_tag="PLS34_10495"
     tRNA            3960..4035
                     /locus_tag="PLS34_10495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3993..3995,aa:Thr,seq:ggt)
BASE COUNT          593 a         1358 c         1557 g          621 t
ORIGIN      
        1 tgttcgccgc ggcgatggcg ccggtctggc tggcggcgac cggccagtcg ggtcggccgc
       61 tgttctggtt cgcgctgctg atggccggtt tcgtcgcctg gacccatcgc agcaacattg
      121 cgcgcatgcg ggccggcaac gagaaccgga tgttccgcgg actcgtgggc cggcgccggt
      181 gagcttcaac gcggccctgc tgcgggcgct cgcgccgggc cgcccggtgt ccggcgaggt
      241 gctcgggcgc gagctcaaca tcagccgcgc ggcggtctgg aaggcggtgc ggcgcctggt
      301 cgagctgggc gtgccgatcg aggctttgct cggtcgcggc tnnnnnnnnn ncccgccgct
      361 ggtgccgctg gacgccgacg cggtgcggcg tgccctgccg acgccggtgg cgcgccgcat
      421 cggcgccctg gaggtgctgg cggagaccga atcgaccaac gccagggtgc tggcggccga
      481 gcgcaaggtc ggggaactcg tggtctgcct cgccgagtac cagtcggccg ggcgcggccg
      541 ccgggggcgg cgctggctcg cgccgccggg cagcggcatc tgcctgtcgg tgggcggccg
      601 cctgccggcg gcgccatcgg actacgcggc gttgcccact gccgtcggcg tcgcctgcgc
      661 cgcggcgctg gaggccctcg gggcggaggc catcggcctg aagtggccga atgacctgct
      721 gctcggcggc cacaagctgg gcggcatcct catcgagttg cggggcgagt cgcagggccc
      781 ggccgtcgtg gcggtcggcc tcggcctgaa cgtgcggctc ggcccggcga cgcgcgagtc
      841 gatcgcggca gccggcggct tgccggccgc ggacctcgcg gcgctggcgg cgtcggcgcc
      901 ggtcgatcgc aaccggctgg cggcggcact ggcggcggcc atcgccggct gcctggagca
      961 tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggggcacga
     1021 agtgcgcgtc gaggacgccg gtggcggccg ggtgggcacc gccctcggcg tggaccatgc
     1081 cggcgcattg ctgctcgcga ccgccgacgg ccggcagcag cgtgtcaccg ccggcgaagt
     1141 gtccctgagg ctgaacgcat gaaactgctg ctggatctcg gcaacacgcg cctgaaggcg
     1201 gcgctcggga tgccggacgg ccggctcgtg ccgctgggcg aggccggcca ccgcgcagcc
     1261 ggcatggcgg tggcgctcga ccatgcgctg ggcgacacgg cgctggcggg tgtgaccggt
     1321 gcgctgtgcg ccaatgtcgc cggggcgccg gccggcgatg cgcttgccac ggcgctgggc
     1381 ggccgcggcg tcgccgtcga attcctgcgt cccggccggg aggccggcgg cgtgcgctgc
     1441 gcttatgcag agccggcgcg cctcggggcg gaccgctggg cggcgctgct gggcgcgcgc
     1501 gggctggaag acggggcgtg cctggtggtg gatgccggct cggcgctgac catcgacgcg
     1561 ctgtcgcccg gcgggcagca cctcggcggg tggattctgc cgggcctcgc catgatggtg
     1621 gcggcgctgg aagcgcgcac cggggacctc ggtgcgctgc ggcgtgcgtc gcaggcgagt
     1681 ccgccggcgg atttcccggc cgacaccgcg ccggcgatgg aaggtggcgc cctgctcgcg
     1741 gcctgcggtg cggtccggtt ggcgcgcaag cgcctggagt gccgctgccg ggtgccggcc
     1801 cggctgttgc tcgccggcgg cgacgccgcc cggctggtga ccgagctgga cggcgccgaa
     1861 ctgcaaccgg acctcgtatt gcgcgggctc gcgcgcctgg ccgccgacaa ggcttgctga
     1921 acggcagctg aatccggcgc ttcccggcgt cgtgggcttt acgaatggtg ccgctcccct
     1981 ggcgccgtat aatttgcctg aaatcgggaa tcgccgttgt tgtccaggag gactgaagat
     2041 catgaaaaag ctgacaatcg cattgctggc cctgcccctc gtcgccctca gcggtgccgc
     2101 cctcgcgggt gatgccgagg ccggcaaggc gaaggccgag agctgcatgg attgccacgc
     2161 cggtgaggat ttcgagggca tgtcccttga agagctcatc gcggccaaca aggctgtgat
     2221 tgcgggcgag cagaagcatc cgccgggcct cgaggacgtg accgtcgagg acgccgagga
     2281 tctcgccgcc tacttctacg ccgaggccgg cggggagtaa ggggctcttt cgacgccgct
     2341 cccgatgcgt cgggggcggc gtcttgcatc ccggcgccgg gccgcgattg ccgggccggg
     2401 agcagcgcca tagaatgcgg cctttggccg gtgtagctca gttggcagag cggctgattt
     2461 gtaatcagca ggtcgcaggt tcgacccctg tcgccggctc cagtccaacg ggcgcactat
     2521 gcaaccggac gccgcgaagc gggtccaagt cgacaagcgt tcgtccatct caagaggaca
     2581 ggaagatgaa gaagctcacg acgatcatga tggcgctgcc gctggccctg ttcagcgccg
     2641 gcgcgctggc gggggatgcg gccgccggtg ccgccaaggc cgaggcctgc atggattgcc
     2701 atgccggcga ggacttcgag ggcatgagcg cggccgagat cgccgacgcc atccgcgcgg
     2761 tgctggccgg ccaggccaag catccgcccg gcgtagacga catcaccgag gcggatgccg
     2821 acgacattgc cgcctacttc tacgccgaag ccggcggcga gtaaggtccc gtggcggcca
     2881 gggaagggcc gccaggaagg atttgacggg gcgcctgctg cggacggcgg gcgcgtccgt
     2941 taaactacgc gcctttcccg cgccaaccta cgaggtttca ggccatgaac aagctcaccc
     3001 ccgcattgct ggcgctgtcg ctggccacct tcagcggagc tgccctggcg ggcgatgccg
     3061 ccgccggcga ggcgaaggcc gccctctgcc tggattgcca ttatgccgat gatttcccga
     3121 atgtttccgc cgaggaagtc gagaccatga ttcgcgccgc acgcgccggc gaggtcaagc
     3181 acggcgagga aatcaacgag ctggccgagg aagacatcgc cgacgtcgcg gcctatttcg
     3241 cccacgaagg ctcgaagtaa ggtggctgcc atgaacaagc tggtgattct gcttgctgcc
     3301 ctgccgctga ctgccctggc cggcacggcg ctggccggtg acccggccgc cggcgaagcc
     3361 aaggccgacg cctgcctcga gtgccacgag ccggactatt tcgtcggcga ggacgcggat
     3421 gcgatcgccg cgatgatccg ggaggtgcgg gccggtgaga tcaagcacaa ggacgacgcc
     3481 ccgcgcgacc tccccgagga agacatcgcc gacgttgcgg cctatttcgc ccacgagggc
     3541 gccaagtagc ccggacacgg tgccggggcc gcctggcccg gccgccggcg agcgccagcc
     3601 gaggccttcc ggtctcggtg atctctgttg acaccccaca gcccgcgttt gataatacgc
     3661 ggcttgcgct tttggggagg ggtacccaag cggtcaacgg gggcagactg taaatctgct
     3721 ggctcagcct tcgaaggttc gaatccttcc ccctccacca cgtgccgcgc ggccggacca
     3781 atggatgcgg cgcggcatgg ggcgggaggc gaaccggagg cgggggcatc agggcacgcg
     3841 cgggcgtagt tcaatggtag aacctcagcc ttccaagctg atgatgtggg ttcgattccc
     3901 atcgcccgct ccagatgcgg gccactgccg cggagcggac gggagatagc aggatcgagg
     3961 cccacatagc tcagtcggta gagcacttcc ttggtaagga agaggtcacc ggttcaaatc
     4021 cggttgtggg ctccagtaac aggcgcggaa tgaaacgagc ggatctgccg ggaaggcgac
     4081 accgcatttt gtagacacac ttaattgatt agtccggagt cgagcaccga tgtccaaggc
     4141 gaaattcgag cgtacgaagc cgcatgtgaa tgtggggacg attgg
//
LOCUS       DAOMPG010000212         4183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8834_length_4183_cov_3.792878, whole genome shotgun sequence.
ACCESSION   DAOMPG010000212 DAOMPG010000000
VERSION     DAOMPG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8834_length_4183_cov_3.792878"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /gene="tagH"
                     /locus_tag="PLS34_10500"
     CDS             <1..1352
                     /gene="tagH"
                     /locus_tag="PLS34_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HRP87929.1"
                     /translation="KQRHILGADSLRVFSVHGGSIGRAPDNDWVLPDPDRYISGHHAR
                     IDYRDGAYYLRDSSTNGVYVNKSEEPVGRGAPLRLYDGDELRMGDYVFDVSIVNVSRD
                     GADDRGDARNTGREAAQATADESAPVLSLKLLGDEADQLEDAALAAAESAPAAAVVTR
                     EQDPEAEAAVAEEFEATMRMADKFSDTQTDVGRAATAAVDRQRVAELRLADSARIRVD
                     QVREKKDQPVFAPSGPRDFTEAVRLLMESAGLDSTQLPRGEEEAAVAVAGKMIRATAE
                     GLVSVLRHRALIKAQFRLTQSTIQSVGNNPLKFAPTTQDALEQLFYEQSEGYMGPVEA
                     VEDSFKYLRAHEAAMVKAMQIAFRDLLDRLDPEVLEERFQRSVKKGGLLSPSAKSKYW
                     EMYQEAYRAIAGFSDENFAAIVGGKFADAYDREMHQLLARLPKDAAPAEPEQGESPD"
     gene            complement(1379..>1766)
                     /locus_tag="PLS34_10505"
     CDS             complement(1379..>1766)
                     /locus_tag="PLS34_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HRP87930.1"
                     /translation="YALDFLWTLRELADWLAGGPGLSRGRRHAGELRVGDTVDSWQVI
                     GLETRRRLTLLFGMKAPGAGILEFEIEPEGDGTRVTATAYWHPRGALGLLYWYPLAPF
                     HGLIFNRMTDAIARRAESGAAAATVS"
     assembly_gap    1767..1776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1777..2832)
                     /locus_tag="PLS34_10510"
     CDS             complement(<1777..2832)
                     /locus_tag="PLS34_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRP87931.1"
                     /translation="MTAGRDVTLVFGASGYIGGHLVPRLLHAGRQVRAVARDAASLDR
                     PGWERAERMSADALRPESLDPVMRDVDTAYYLVHSMGAGKDFGRLDLECADNFAAAAA
                     RAGVRRIVYLGGLIPEGARSEHLLSRKETGDRLRAGPVPVTEIRAGIIVGPGSAAFEV
                     IRDLVNALPAMVTPRWVRARTPPIALDNLLEYLVRIPEHAAPGHEIFDAAGPEELSYE
                     QLMRIFGELVGRSPVIVPVPVLTPTLSSYWLGLVTTVPTSVARALIGGLAHHIRANPE
                     PLRARIPQRLLDYREAVTAALDAERRHAVAARWTEGALMYRNYRHDYAYYAKRAAGSA
                     VTRASADDVWRVVTAIGG"
     gene            complement(2832..3761)
                     /locus_tag="PLS34_10515"
     CDS             complement(2832..3761)
                     /locus_tag="PLS34_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRP87932.1"
                     /translation="MKVALFGGTGFVGSYLVDSLLAHGHVPRLLVRAGSEDKVAQRAR
                     CEIVTGDIHEPAAVATCLEGADAAIYNIGLIRELPKQGITWEAMHFAGAKAAIDAARN
                     AGVRRFLLMSANGVKPEGTGYQSTKYRAEQYLAASGLDWTVFRPSIVFGDPRGRLEFC
                     SMLRDQMIKPPIPAPLFHQGLWPGGAGRFLMSPVAVEDVAEAFVRSLATPASCGRVLP
                     LGGPEALEWREILHIIAAGAGRKKVMVPAPVGAVRAAAALLDGFEWFPVTRAQLQMLV
                     EGNACDGSEAWRMFGIEPVRFTPAALGYLRDDS"
     gene            complement(3758..>4183)
                     /locus_tag="PLS34_10520"
     CDS             complement(3758..>4183)
                     /locus_tag="PLS34_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRP87933.1"
                     /translation="GESGRRGSAEHGAMKTIIYFDGGCPVCAHGAAHWQRRDWARRTV
                     WVDLMEQPEALAAEGVSFATAMETLHVRDRQGRLVVGGHAFLELWDQLSGWRLLSRLI
                     RALRLERAFDRAYRWHSRGRFQKRCGAGGCPVPGAGSGR"
BASE COUNT          649 a         1534 c         1454 g          536 t
ORIGIN      
        1 gcaagcagcg gcacattctc ggcgctgaca gcctgcgcgt gttcagcgtg cacggtggat
       61 cgatcggccg cgcgcccgac aatgactggg tcctgccgga tcccgaccgg tacatctccg
      121 ggcatcatgc ccggatcgat tatcgcgacg gcgcgtacta cctgcgcgac agcagcacca
      181 acggcgtgta cgtcaacaag tcggaggagc cggtcgggcg tggggcgccg ctgcggctct
      241 atgacggcga cgagctgcgc atgggtgact acgtgttcga cgtcagcatc gtcaacgtca
      301 gccgcgacgg cgcggacgac cgcggcgatg cccgcaatac cggccgggaa gcggcgcagg
      361 ccactgcgga cgaaagcgcc ccggtgctgt ccctcaagtt gctcggcgac gaggcggacc
      421 agctggagga cgcggcgctg gcggccgcgg agtccgcgcc ggccgcggcc gtcgtcacca
      481 gggaacagga cccggaagcc gaggccgccg tcgccgagga attcgaggcg accatgcgca
      541 tggccgacaa gttcagcgac acgcagaccg atgtcggccg cgcggccacc gcggcggtcg
      601 atcgccagcg ggtcgccgag ctgcggctgg ccgactcggc gcgcatccgg gtggaccagg
      661 tgcgcgagaa gaaggaccag ccggtgttcg ccccgagcgg gccgcgggat ttcaccgagg
      721 cggtgcgcct gctgatggaa tccgccgggc tggactcgac ccagctgccg cgcggcgagg
      781 aggaagcagc ggtcgcggtg gcgggcaaga tgatccgcgc gacggccgag ggcctggtgt
      841 ccgtgctgcg gcatcgcgcg ctcatcaagg cgcagttccg tctcacccag tcgaccatcc
      901 agtcggtcgg caacaacccg ctgaaattcg cgccgacgac ccaggatgcg ctcgagcagc
      961 tgttctacga gcagagcgag ggatacatgg ggccggtcga agcggtcgaa gacagcttca
     1021 agtacctgcg ggcccacgag gcggccatgg tgaaggcgat gcagatcgcg ttccgcgacc
     1081 tgctcgaccg gctggacccc gaggtgctgg aggagcgctt ccagcgctcg gtgaagaagg
     1141 gtggattgct gtcgccgtcc gccaagtcga agtactggga aatgtaccag gaagcctacc
     1201 gcgccatcgc cgggttcagc gacgagaact ttgccgcgat cgtcggcggc aagttcgccg
     1261 acgcctacga ccgcgagatg caccagctgc tggcgcgcct ccccaaggac gccgcgccgg
     1321 ccgagccgga gcagggcgag tcgcccgact agtcgaaggc gcgtcgccgc gccaccggtc
     1381 aactcaccgt ggcggcagcc gcgccggact cggcacgccg cgcgatggcg tcggtcatgc
     1441 ggttgaagat cagcccgtgg aagggcgcca gcggatacca gtacagcagc ccgagggccc
     1501 cgcgcgggtg ccagtaggcg gtggccgtga cgcgggttcc gtcgccctcg ggctcgatct
     1561 cgaattcgag gatgccggcg ccgggggctt tcatgccgaa cagcagcgtc agcctgcgcc
     1621 gcgtctccag cccgatgacc tgccacgaat ccaccgtgtc gcccacccgc agctcgccgg
     1681 catgccggcg cccgcgactc agcccgggac cgccggccag ccagtccgcg agctcgcgca
     1741 gagtccacag gaagtccagc gcgtaannnn nnnnnncccg ccgatcgcgg tcaccacgcg
     1801 ccagacgtcg tcggccgacg cgcgggtcac ggcactgccg gcggcgcgtt tggcgtagta
     1861 cgcgtaatcg tggcgataat tgcggtacat cagcgcgccc tcggtccagc gcgccgcgac
     1921 cgcatgccgc cgctcggcgt cgagcgccgc ggtgacggcc tcgcggtaat ccagcagccg
     1981 ctgcggtatg cgggcgcgca acggctcggg gttggcgcgg atgtgatgcg cgagcccgcc
     2041 gatcagcgcg cgcgccaccg aggtgggcac ggtcgtgacg agaccgagcc agtaggagga
     2101 cagggtcggc gtcagcaccg gcaccggcac gatcaccggg ctgcggccga cgagctcgcc
     2161 gaagatgcgc atcagctgct cgtaggaaag ctcctccggc cccgccgcat caaagatctc
     2221 gtggcccggt gcggcgtgct ccgggattcg caccaggtac tccagcaggt tgtcgagcgc
     2281 gatcggcggc gtgcgcgccc gcacccagcg cggcgtcacc atggcgggca atgcgttgac
     2341 caggtcgcgg atgacctcga aggccgccga gcccggaccg acgatgatgc cggcgcggat
     2401 ttcggtcacc ggtaccggcc cggcgcgcag ccggtcgccg gtctccttgc gcgacagcag
     2461 gtgctcggag cgcgcgcctt ccggaatcag gccgccgagg tagacgatgc gccgcacgcc
     2521 ggcgcgcgcc gccgcggccg cgaagttgtc ggcgcactcg agatcgagcc gcccgaagtc
     2581 cttgcccgcg cccatggagt gcaccaggta atacgccgtg tccacgtcgc gcatgaccgg
     2641 gtcgagcgac tccggccgca aggcgtcggc cgacatccgc tcggcgcgct cccagccggg
     2701 ccgatcgagc gacgccgcgt cgcgggccac ggcccgcacc tgccggccgg cgtgcagcaa
     2761 tcgcggcacc aggtggccgc cgatgtaacc gctggcgccg aacaccagcg tgacgtcgcg
     2821 cccggcggtc atcaggagtc gtcgcgcagg tagccgagcg cggcgggggt gaaacgcacc
     2881 ggctcgatgc cgaacatccg ccacgcctcg ctgccgtcgc aggcattgcc ctccacgagc
     2941 atctgcaact gggcgcgcgt caccgggaac cactcgaagc cgtcgagcag ggccgccgcc
     3001 gcgcgcaccg cgccgaccgg cgccggcacc atcactttct tcctgccggc gcccgccgcg
     3061 atgatgtgca ggatctcgcg ccactccagc gcctcgggcc cgcccagcgg caacacgcgg
     3121 ccgcagctgg ccggcgtcgc aaggctgcgc acgaaggcct ccgccacgtc ctccaccgcc
     3181 accggggaca tcaggaagcg tcccgcgccg cccggccaca ggccctggtg aaacagcggc
     3241 gccgggatcg gcggcttgat catctggtcg cgcagcatgc tgcagaactc cagccggccg
     3301 cgcgggtcgc cgaacacgat cgacggccgg aacaccgtcc agtcgagccc gctggccgca
     3361 aggtactgct cggcgcggta cttggtcgac tggtagccgg tgccctcggg cttcacgccg
     3421 ttggcgctca tcagcaggaa gcgccgtacg ccggcattgc gcgcagcgtc gatggccgcc
     3481 ttcgcgcccg cgaagtgcat cgcctcccag gtgatgccct gcttcggcag ctcgcggatc
     3541 agcccgatgt tgtagatcgc cgcgtccgcg ccctccaggc aggtcgccac cgccgccggc
     3601 tcgtggatgt cgcccgtcac gatctcacag cgcgcgcgct gcgcgacctt gtcctcgctg
     3661 cccgcgcgca ccagcaggcg cggcacgtgg ccgtgcgcga gcaggctgtc gaccaggtag
     3721 ctgccgacga atcccgtgcc gccgaacagg gccaccttca tcggccgctc cccgcgcccg
     3781 gcaccgggca gccgcccgcg ccgcagcgct tctggaaacg tccccggctg tgccaccggt
     3841 acgcacgatc gaaggcccgc tccagccgca aggcgcggat cagccgcgac agcagccgcc
     3901 agccggacag ctggtcccac agctcgagaa aggcgtgccc gccgaccacc agccggccct
     3961 gccggtcgcg cacgtgcagc gtttccatcg ccgtggcgaa gctcacgccc tcggccgcca
     4021 gcgcctccgg ctgctccatg aggtcgaccc acaccgtgcg ccgcgcccag tcacggcgtt
     4081 gccagtgcgc cgcgccgtgc gcgcacaccg gacagccgcc gtcaaagtag atgatcgttt
     4141 tcatcgcccc gtgttcggcg ctcccccgcc ggccggattc gcc
//
LOCUS       DAOMPG010000213         4164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_8879_length_4164_cov_5.168411, whole genome shotgun sequence.
ACCESSION   DAOMPG010000213 DAOMPG010000000
VERSION     DAOMPG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8879_length_4164_cov_5.168411"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1279)
                     /gene="fabB"
                     /locus_tag="PLS34_10525"
     CDS             complement(65..1279)
                     /gene="fabB"
                     /locus_tag="PLS34_10525"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRP87934.1"
                     /translation="MRRVVVTGFGIVSSIGNNRDEVLDSLRSGRSGIRFNEEYRELGF
                     RSHVYGPVEIDLAAMIDRKLKRFMSDSAGYTYVAMQQAIAQAGLEERDISNPRSGLIV
                     GSGGASTENVFWTADTVRQKGARKVGPYMVTRCMSSAIAANLATAFKVKGVSYTISSA
                     CATSAHCIGNGAELIQLGKQDVVFAGGAEELHWGTSVMFDGMGALSSKYNDTPEKASR
                     TYDAERDGFVISGGGGIMILEELEHARARGANIICELVGYGATSDGADMVQPSGEGAV
                     RCMRMALDGVDGPVDYLNTHGTSTPVGDIKELEAIREVFGAESPPVASTKSLAGHALG
                     AAGVNEAVHCLLMMQHDFIAASANIERLDPAAEGFDIVRERRDGVRLDTVMSNSFGFG
                     GTNATLVFRRWA"
     gene            complement(1289..1801)
                     /gene="fabA"
                     /locus_tag="PLS34_10530"
     CDS             complement(1289..1801)
                     /gene="fabA"
                     /locus_tag="PLS34_10530"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HRP87935.1"
                     /translation="MTKNSYSYEELLQAGHGELFGKDRARAPLPPMLMMDRISRITAD
                     GGAHGLGQIVAELDIKPELWFFKCHFESDPVMPGCLGLDAMWQLIGFFLCWSGGQGRG
                     RALGVGEVKFTGQVLPTAKLVTYRIDMKRVLLRRLIMGIGDATMSVDGREIYTATDLK
                     VGLFTSTESF"
     gene            complement(1943..3583)
                     /locus_tag="PLS34_10535"
     CDS             complement(1943..3583)
                     /locus_tag="PLS34_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HRP87936.1"
                     /translation="MTLQARGPVARLMLAGTIALLFALGGCSGDDGDDGTPGAPGAAG
                     ADGINCWDLNANGVADAEEDVNDDGVVDVLDCRLPPPSIADAATLHQAYFADRSYEGT
                     ESCLVCHGPIGDDILTTGHFKWEGVAAGLEGVEGEIHGKNDIINNFCVAVPSNEGRCT
                     QCHIGYDYADQSYDFGNPRLIDCFACHDQTGTYTKAAPAAGRPPASVDLQAVAQSVGL
                     NNGVPTRKACLFCHAGAGGGDNVKHGDLSSDLIATTREYDVHMATDGANASCVLCHDV
                     KRDLDGNVLSHGIGGMPYHSVDEGNMRQCDDCHSPTVHVGRPVESILNTHDRLACQAC
                     HIPAISRKLPTKTEWYWADTGQDISPIPLDPDTGKAAYDKMKGTFNWELNVRPVLRYY
                     DGKSIKSLIGVSDNVAALPSEDGRVYLGGPAADYTNPNAKIYPFKKLIGNQPGDAQNG
                     TILVPHLFGLAGGPNPYWVAYDWDLALRDGAAITGQTYSGEYEFIDTVSYWAVNHEVA
                     PAEMALGRGGCDDCHGNGQIDWAGLGYSDDPVAGGTRP"
     gene            complement(3752..>4164)
                     /locus_tag="PLS34_10540"
     CDS             complement(3752..>4164)
                     /locus_tag="PLS34_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016709821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP87937.1"
                     /translation="IMGMSLTIMSLMGMLALTGVVVNDSLVLVDYINRRRAEGMPLME
                     AVSTAGAARLRPVLLTSLTTFAGLTPLIFEKSTQAQFLIPMAVSLGFGILFATFITLL
                     LVPLNYLVLEDLRGLARRIFGRRGGAVAEAGARS"
BASE COUNT          664 a         1397 c         1371 g          732 t
ORIGIN      
        1 caggtttagc gagcgaaacc tgtctctgac cccggttttt cctgaccccg gttttcggtt
       61 ttgcctaggc ccagcggcgg aacacgagcg tggcgttggt gccgccgaag ccgaagctgt
      121 tggacatgac ggtgtccagg cgcacgccgt cgcgccgctc gcgcacgatg tcgaagcctt
      181 cggcggccgg gtccagccgc tcgatgttgg ccgacgcggc aatgaagtcg tgctgcatca
      241 tcagcaggca gtgcacggcc tcgttgacgc ctgcggcgcc cagcgcgtgg cccgccagcg
      301 acttggtcga ggccaccggc gggctctcgg cgccgaatac ctcgcggatc gcctccagct
      361 ccttgatgtc gccgaccggc gtgctggtgc cgtgggtgtt gaggtaatcc accgggccgt
      421 cgacgccgtc cagcgccatg cgcatgcagc gcaccgcgcc ctccccggag ggttgcacca
      481 tgtcggcgcc atcggaggtc gccccgtagc ccaccagctc gcagatgatg ttcgcaccgc
      541 gcgcccgggc gtgctcgagt tcctccagga tcatgatgcc gccgccgccc gagatgacga
      601 agccgtcgcg ctccgcatcg taggtccgcg acgccttctc cggcgtgtcg ttgtacttcg
      661 aggacagcgc gcccattccg tcgaacatca ccgaggtgcc ccagtgcagc tcctcggcgc
      721 cgccggcgaa cacgacgtcc tgcttgccga gctggatcag ctccgcgccg ttgccgatgc
      781 agtgcgcgct ggtcgcgcag gcggaactga tggtgtagct cacgcccttc accttgaagg
      841 ccgtggccag gttggcggcg atcgcggacg acatgcagcg cgtcaccatg tacggtccca
      901 ccttgcgggc gcccttctgg cgcaccgtgt ccgcggtcca gaacacgttc tccgtcgagg
      961 cgccgccgga accgacgatc agcccgctgc gcggattgct gatgtcgcgt tcctccaggc
     1021 cggcctgcgc gatggcctgt tgcatggcca cataggtgta gccggccgag tcgctcatga
     1081 agcgcttcag cttgcgatcg atcatcgccg cgagatcgat ctccaccggt ccgtagacat
     1141 ggctgcggaa accgagctcg cgatactcct cgttgaagcg gatgccggaa cggccgctgc
     1201 gcagcgagtc cagcacttca tcgcgattgt tgccgatgct cgatacgatg ccgaaaccgg
     1261 tgacgacgac gcggcgcatg atgccccctc agaaggattc cgtagaagta aacaggccga
     1321 ccttgaggtc ggtcgccgtg tagatctcgc ggccgtccac gctcatggtg gcgtccccga
     1381 tccccatgat cagccggcgc agcaggaccc gcttcatgtc gatccggtag gtgacgagtt
     1441 tggccgtggg cagcacctgg ccggtgaact tcacctcgcc gacgccgagc gcgcggccgc
     1501 ggccctggcc gccgctccag cacaggaaaa acccgatcag ctgccacatg gcgtcgagcc
     1561 ccaggcaacc cggcatcacc gggtcggact cgaaatggca cttgaaaaac cagagctcgg
     1621 gcttgatgtc gagttctgcg acgatctggc cgaggccgtg tgcgccgccg tcggcggtga
     1681 tgcggctgat gcggtccatc atgagcatgg gcggcagcgg cgcgcgggca cggtccttgc
     1741 cgaacagctc gccatggccg gcctgcagca attcctcgta gctgtacgag tttttcgtca
     1801 tgtatgcgcc ccgctgcgcc agtgtggcag ggcggtattg tgcagcaaaa tcggcgaaat
     1861 tatttcttgc ggcgcgtcat cggcacgaaa aaacggggcg cccgcgccgc agcgcgaacg
     1921 cccctcccct ggagaaggtc aactagggac gtgtgccgcc ggcgaccgga tcatcgctgt
     1981 agccgaggcc ggcccagtcg atctggccat tgccgtggca gtcgtcgcat ccgccccggc
     2041 cgagcgccat ctccgccggc gcgacctcgt ggttcaccgc ccagtagctc accgtgtcga
     2101 tgaactcgta ctcgcccgaa tacgtctggc ccgtgatcgc cgcaccgtcg cgcagcgcca
     2161 ggtcccagtc gtacgcgacc caatagggat tgggcccgcc ggcgagcccg aacaggtgcg
     2221 gcacgaggat ggtgccgttc tgggcgtcgc ccggctggtt gccgatcagt ttcttgaacg
     2281 gatagatctt ggcgttcggg ttggtgtaat cggccgccgg cccgccgaga tagactctgc
     2341 cgtcctcgga gggaagagcc gccacgttat cgctgacgcc gatcagcgac ttgatggact
     2401 tgccgtcgta atagcgcagt accggccgga cgttcagctc ccagttgaag gtgcccttca
     2461 tcttgtcgta ggccgccttg ccggtatccg ggtcgagcgg aatcggactg atgtcctggc
     2521 cggtgtccgc ccaataccac tcggtcttgg tcggcagctt ccgcgagatc gccggaatgt
     2581 gacaggcctg gcacgcgaga cggtcgtggg tgttgaggat gctctccacc ggccggccga
     2641 cgtggacggt ggggctgtgg cagtcgtcgc actgccgcat gttgccctcg tccaccgagt
     2701 ggtagggcat gccgccgatg ccgtgcgaca gcacattgcc gtcgaggtca cgcttcacgt
     2761 cgtggcagag cacgcaactg gcgttagcgc cgtccgtcgc catgtgcacg tcgtactcgc
     2821 gggtcgtggc aatcaggtcg gaggacaggt cgccgtgctt gacgttgtcg ccgccgccgg
     2881 cgccggcatg gcagaacagg caggccttgc gggtcggcac gccgttgttc aggccgacgc
     2941 tctgcgccac cgcctgcagg tcgactgagg ccggcgggcg gcctgcagcc ggcgccgcct
     3001 tggtgtaggt gccggtctgg tcatggcagg cgaagcagtc gatcagcctg ggattgccga
     3061 aatcgtagct ctggtcggca tagtcgtagc cgatatggca ctgggtgcag cgcccctcat
     3121 tgctgggcac cgccacgcag aagttgttga tgatgtcgtt cttgccgtga atctcgccct
     3181 cgacgccttc gaggcctgcg gcgacgccct cccacttgaa gtgccccgtg gtcaggatgt
     3241 cgtccccgat cgggccgtgg cagaccaggc aggactcggt gccctcgtag ctccggtcgg
     3301 cgaaataggc ctggtgaagc gtggctgcgt cggcgatgga cggcggcggc aggcggcagt
     3361 cgagcacgtc cacgacgcca tcatcgttga cgtcctcttc cgcgtctgcg acgccgttgg
     3421 cgttgagatc ccaacaattg atgccgtccg cgccggctgc acccggcgcg cccggcgtgc
     3481 cgtcgtcgcc gtcgtcgccg ctgcagccgc ccagggcgaa cagcaatgcg atcgtcccgg
     3541 ccagcatgag tcgggccacc ggcccgcgtg cctggagagt catgacaggg gtcctctgca
     3601 aattccgcga cacccccggg cagccccggg gcccgcacct gattcaaata actcttattt
     3661 agaatgattt gaattaggtg cagcgcaaat ttgcgcccac cggacgctgt catgtttgat
     3721 ctggatcaac gcgccccgta gggaaatatc cctagctgcg tgcgccggcc tcggccaccg
     3781 ccccgccccg acggccgaaa atccgccgcg cgagaccgcg caggtcctcc agcaccaggt
     3841 agttcaacgg caccagcagc agggtgatga aggtggcgaa caggatgccg aagcccagcg
     3901 acaccgccat cgggatcagg aactgcgcct gggtggactt ctcgaagatc agcggcgtga
     3961 ggccggcgaa ggtggtcagc gaggtgagca gcaccggccg caggcgggcg gcacccgccg
     4021 tgctgaccgc ctccatcagc ggcatgccct cggcgcggcg gcggttgatg tagtccacca
     4081 gcaccaggct gtcgttcacc accacgccgg tcagcgccag catgcccatc aggctcatga
     4141 tggtgaggct catgcccatg atca
//
LOCUS       DAOMPG010000214         4089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9081_length_4089_cov_6.562469, whole genome shotgun sequence.
ACCESSION   DAOMPG010000214 DAOMPG010000000
VERSION     DAOMPG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9081_length_4089_cov_6.562469"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..436)
                     /gene="rpsM"
                     /locus_tag="PLS34_10545"
     CDS             complement(80..436)
                     /gene="rpsM"
                     /locus_tag="PLS34_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRP87938.1"
                     /translation="MARIAGINIPANKHIVIALTHIFGVGNSRAKKICEAAGVPPDTK
                     VRDLTEPDVEKLRSEVAKIEVEGDLRRETSMNIKRLMDLGCYRGIRHRRGLPLRGQRT
                     RTNARTRKGPRRPVRK"
     gene            complement(507..620)
                     /gene="rpmJ"
                     /locus_tag="PLS34_10550"
     CDS             complement(507..620)
                     /gene="rpmJ"
                     /locus_tag="PLS34_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRP87939.1"
                     /translation="MKVRASVKKICRNCKIIRRGGVIRVICSDPRHKQRQG"
     gene            complement(645..1970)
                     /gene="secY"
                     /locus_tag="PLS34_10555"
     CDS             complement(645..1970)
                     /gene="secY"
                     /locus_tag="PLS34_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRP87940.1"
                     /translation="MNPATALGEAARLGDLRKRLFFLLGALVVYRIGTFIPVPGIDPV
                     ALARFFQEQQGTILSVFNMFSGGALERLSILALGIMPYISASIIMQMMSVVVPSLAAM
                     KKEGEAGRRKITQYTRYGTLGLATFQSIGAAVAFQNQGVVINPGPQFVFIACVTLVTG
                     TMFLMWLGEQVTERGIGNGISMIILAGIVAGLPSALGGTLELVRNGEMPAALALILFF
                     LVLAVTAFVAFVERGQRRIAVNYAKRQQGRRMYQGQTTHLPFKLNMAGVIPPIFASSL
                     ILFPATVAQWFGTAESMGWLQVLASSLSPGQPVYTLMYAGLIIFFCFFYTALVFNSRE
                     TADNLKRSGAFIPGIRPGQQTAEYIDSVLTRLTFWGAMYITAICLLPEFMIMYWNVPF
                     YFGGTSLLIIVVVSMDFMSQLQAHMMSHQYDGLLKKANLKGYGRAGQVR"
     gene            complement(2043..2474)
                     /gene="rplO"
                     /locus_tag="PLS34_10560"
     CDS             complement(2043..2474)
                     /gene="rplO"
                     /locus_tag="PLS34_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRP87941.1"
                     /translation="MRLNDIKPAEGSRKEGKRLGRGHSAGQGKTSGRGVKGQHARSGG
                     YHKVGFEGGQMPLQRRLPKIGFNSRMGRFSGELRLGELNGFEGEVDLLALKAAGLVGQ
                     RVKRVKIIASGKVEKALTIKGIPVTPGARAAIEAAGGTIEE"
     gene            complement(2476..2676)
                     /gene="rpmD"
                     /locus_tag="PLS34_10565"
     CDS             complement(2476..2676)
                     /gene="rpmD"
                     /locus_tag="PLS34_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRP87942.1"
                     /translation="MAKTNANPRQLRVKLVKSLHGRLPRHKACVSGLGLRRMHHSVVV
                     ADTPENRGMINKVSYLLQVEEV"
     gene            complement(2681..3187)
                     /gene="rpsE"
                     /locus_tag="PLS34_10570"
     CDS             complement(2681..3187)
                     /gene="rpsE"
                     /locus_tag="PLS34_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRP87943.1"
                     /translation="MAAPERNSAGDDLIEKLVTVNRVAKVVKGGRQFGFTALTVVGDG
                     SGRVGYGYGKAREVPVAIQKAMQAARKNMRRIQLKDDTLQYATEGRHGATKVYMQPAS
                     DGTGVIAGGAMRAVFECAGVRNVLAKSYGSRNPINVVQATIKGLSGIRSPAQIAAKRG
                     KTVEEILG"
     gene            complement(3213..3566)
                     /gene="rplR"
                     /locus_tag="PLS34_10575"
     CDS             complement(3213..3566)
                     /gene="rplR"
                     /locus_tag="PLS34_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRP87944.1"
                     /translation="MEKKIARIRRARRARSKIRELGYNRLSIHRTPRHIYAQIISPDG
                     GKVLATASTVQPAVREGIEKTGNIAAAQAVGRAIAEAAKAAGVSKVAFDRSGFRYHGR
                     VKALADAAREGGLEF"
     gene            complement(3580..>4089)
                     /gene="rplF"
                     /locus_tag="PLS34_10580"
     CDS             complement(3580..>4089)
                     /gene="rplF"
                     /locus_tag="PLS34_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRP87945.1"
                     /translation="VSLPKGVEFKLDGGQAVVKGPKGTLSLPLDPSVEVVQEGAELQV
                     RTPGTEKPSGAMAGTTRALLSNMVQGVSQGFQKKLELVGVGYRAQAQGKVLNLTLGFS
                     HPVAYPVPEGITIETPSQTEVVVSGIDRQQVGQVAADIRGYRPPEPYKGKGVRYSGER
                     VVMKEAKKK"
BASE COUNT          627 a         1393 c         1315 g          754 t
ORIGIN      
        1 ttcttgcgca ccttgggggc ttgcttggcc attcgttcga aaccttcagc gttggcgata
       61 atgccgcttc agcggcgcct tacttcctga ccgggcggcg cggacccttc cgcgtccggg
      121 cgttggtacg ggtgcgctgc ccgcgcagcg gcaggccgcg acgatggcgt atgccgcggt
      181 agcagccgag gtccatcagc cgcttgatgt tcatcgacgt ttcgcgccgc aggtcgccct
      241 cgacctcgat cttggcgacc tcgctgcgca gcttttccac gtccggctcg gtgaggtccc
      301 ggaccttggt gtcaggcggg acgcctgccg cttcgcaaat cttcttcgcg cgcgagttgc
      361 cgaccccgaa aatgtgggtc agcgcgatga ctatgtgctt gttggctggg atgttgatcc
      421 cggcaatacg ggccattaac gccgtctcct gtcgctcacg cgaagccgat cattgtgcct
      481 gatccggcgc tcgccttcaa tgtctgttaa ccctgccgct gcttgtggcg cggatccgag
      541 cagatcaccc ggatcacgcc tccgcggcgg atgatcttgc agttcctgca gatcttcttg
      601 accgatgcac gcactttcat tgctctctcc tggttccgcg ccggtcagcg gacttgcccg
      661 gcacggccgt agcccttcag gttggccttc ttcagcaggc cgtcgtactg gtgactcatc
      721 atgtgggcct gcagctggga catgaagtcc atgctgacga ccacgatgat cagcagcgaa
      781 gtgccgccga agtagaacgg cacgttccag tacatgatca tgaactccgg cagcaggcag
      841 atggcggtga tgtacatcgc cccccagaac gtcagccggg taagcacgct gtcgatgtat
      901 tcggcggtct gctgccccgg gcggatgccg gggatgaacg cgccggaacg cttcaggttg
      961 tcggccgtct cgcgcgagtt gaacacgagc gccgtgtaga agaagcagaa aaagatgata
     1021 aggccggcat acatcagcgt atagaccggc tggccggggg acaggctcga ggccagcacc
     1081 tgcagccagc ccatgctctc cgcggtgccg aaccactgcg ccaccgtcgc cgggaacagg
     1141 atcaggctcg aggcgaagat cggcgggatg acgccggcca tgttgagctt gaacggcagg
     1201 tgggtggtct ggccctggta catgcgccgg ccctgctggc gcttggcgta gttcaccgcg
     1261 atgcggcgct ggccgcgctc gacgaaggcc acgaaggcgg tgacggccag taccaggaag
     1321 aacaggatca gcgccagcgc cgccggcatt tcgccgttgc ggaccagctc cagcgtaccg
     1381 cccagcgccg acggcaggcc ggcgacgatg ccggcgagga tgatcatcga gatgccgttg
     1441 ccgatgccgc gctcggtcac ctgctcgccg agccacatca ggaacatggt gcccgtgacc
     1501 agggtgacgc aggcgatgaa cacgaactgc gggcccgggt tgatgaccac gccctggttc
     1561 tggaaggcta ccgccgcgcc gatcgactgg aaggtcgcca ggccgagcgt gccgtaacgc
     1621 gtgtactggg tgatcttccg gcggccggcc tcaccttctt tcttcatggc ggccagcgac
     1681 ggcaccacca ccgacatcat ctgcatgatg atggaggcgg agatgtaggg catgatgccg
     1741 agcgccagga tcgacaggcg ttcgagcgca ccgcccgaga acatgttgaa cacgctcagg
     1801 atggtgccct gctgctcctg gaagaagcgc gccagcgcga cgggatcgat gccgggaacg
     1861 gggatgaacg tcccgatccg gtacaccacg agggcgccca gcaggaaaaa cagccgcttg
     1921 cggaggtcgc ccagtcgggc ggcctcgcca agggccgtag ccggattcat gctcgttggt
     1981 gacttcgcca cttcagctcc gcgtaccgtc ccaggcgccg gtggcgcccg ggtaaaagtc
     2041 cgttattcct cgatcgttcc gccggcggcc tcgatcgccg cgcgcgcgcc cggggtcacc
     2101 gggatgccct tgatggtcag cgccttctcg accttgccgg aggcgatgat cttgacgcgc
     2161 ttgacccgct gcccgaccag gccggcagcc ttcagcgcca gcaggtccac ctcgccctca
     2221 aagccgttga gctcgccgag acgcagctcg ccggagaacc ggcccatgcg cgagttgaag
     2281 ccgatcttcg gcaggcggcg ctgcagcggc atctggccgc cttcgaaacc gaccttgtgg
     2341 tagccgccgg atcgggcgtg ctggcccttg acgccgcggc cgctggtctt gccctggccg
     2401 gccgaatggc cacggcccag gcgcttgcct tccttgcggc tgccctcggc gggcttgatg
     2461 tcattgaggc gcatctcaga cttcctctac ctgcaacagg tacgagacct tgttgatcat
     2521 gccccggttc tccggggtat cggccaccac cacgctgtgg tgcatgcgcc gcagccccag
     2581 gccggacacg caggccttgt gccggggcag gcgcccgtgg aggctcttga ccagcttgac
     2641 ccgcagctgg cggggatttg cgttcgtctt tgccatggtc ctaacccagg atctcctcga
     2701 ccgtcttgcc gcgcttggcc gcaatctgcg ccggcgagcg gatgcccgac agccccttga
     2761 tggtggcctg cacgacgttg atcgggttgc gcgagccgta gcttttcgcc agcacgttgc
     2821 gcacgccggc gcactcgaac acggcgcgca tggcgccgcc ggcgatcact ccggttccgt
     2881 cggatgccgg ctgcatgtac accttggtcg cgccgtggcg cccctcggtc gcgtactgca
     2941 gggtgtcgtc cttgagctgg atgcgccgca tgttcttgcg cgccgcctgc atggccttct
     3001 ggatggcgac cgggacttcc cgcgccttgc catagccgta gccgacccgc ccggagccgt
     3061 cgccgaccac ggtcagcgcc gtgaagccga actggcgtcc gcccttcacg accttcgcga
     3121 cacgattgac ggtgacgagc ttctcgatga gatcgtcgcc ggcgctattt ctttctggtg
     3181 ctgccattgt tcaactgtcc ccgggcgtat gcctagaact cgagaccgcc ctcgcgggcc
     3241 gcgtcggcga gggccttgac cctgccgtga tagcggaacc ccgagcggtc gaatgccacc
     3301 ttgctcacgc cggcggcctt ggccgcctcg gcgatcgccc ggccaacggc ctgggcggct
     3361 gcgatattgc cggtcttctc gataccctcg cgcacggccg gctgcaccgt cgatgcagtc
     3421 gccagcacct tcccgccgtc cggcgagatg atctgcgcat agatgtggcg cggggtacgg
     3481 tgaatgctga gacggttgta tcccagctcc cggatctttg accgggcgcg gcgcgcgcgc
     3541 cgaatgcgtg caattttctt ttccatgcct cgcctcgccc tacttcttct tcgcttcctt
     3601 catgaccacg cgctcacccg agtaccgcac gcccttgccc ttgtaaggct ccggcggacg
     3661 gtagccacgg atgtccgcgg ccacctggcc cacttgctgg cgatcgatgc cgctgaccac
     3721 gacttccgtc tggctgggcg tctcgatcgt gatgccctcg gggaccgggt aggccaccgg
     3781 gtgcgagaaa ccgagcgtca ggttcaggac cttgccctgg gcctgcgcgc ggtaaccgac
     3841 gccgaccagc tcgagcttct tctggaagcc ctgggagacg ccctgcacca tattggacaa
     3901 cagcgcccgg gtcgtgccgg ccatcgcgcc ggacggcttc tcggtccccg gagtccggac
     3961 ctggagctcc gcgccctcct ggacgacctc gaccgacggg tcgagcggca gggacagcgt
     4021 cccctttgga cccttcacca cggcctgccc gccgtcgagc ttgaactcga cgcccttggg
     4081 caacgacac
//
LOCUS       DAOMPG010000215         4040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9224_length_4040_cov_3.934253, whole genome shotgun sequence.
ACCESSION   DAOMPG010000215 DAOMPG010000000
VERSION     DAOMPG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9224_length_4040_cov_3.934253"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="proA"
                     /locus_tag="PLS34_10585"
     CDS             <1..142
                     /gene="proA"
                     /locus_tag="PLS34_10585"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654021.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HRP87946.1"
                     /translation="FADGGEFGMGAEIGIATGRIHARGPVGAEQLTSYKYQVSGAGQL
                     RP"
     gene            213..716
                     /locus_tag="PLS34_10590"
     CDS             213..716
                     /locus_tag="PLS34_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP87947.1"
                     /translation="MRNAPLAALAALSFVVFGAPLQAQEDAPVIQDGSTVSIEYTLKL
                     DDGSTADTNAGREPLVYVQGEQQILPALEEKLLGMKADETREVTLTPEEGYGPVHEEA
                     LQTVPLDIIPEEARTVGARLVGQGPEGQPLHAVVKEINEDSAVVDLNHPLAGQKLHFS
                     IKVLSIQ"
     gene            871..2073
                     /locus_tag="PLS34_10595"
     CDS             871..2073
                     /locus_tag="PLS34_10595"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HRP87948.1"
                     /translation="MKVLLLDDDPFVLRLLERQLANLGVEVRHACERAELALAQLAEG
                     SADVDVVFCDLQMPGMDGVEFLRNLVAIDDPPALVLVSGEDTRVLESAERLARAHHLV
                     VLGALHKPFDMGQLRRVIERHEALARDPTLTGLPSVYSPEELGESIRRGELLNHYQPK
                     VELRTGRVVGVEALVRWQHSRDGLVFPDRFVPIAEQSGLIDALGRAVLGNALAQLRHW
                     RDAGLELHVAVNVSMASLVNLAFPDYVLRETAAAGVPASALVLEITESRLMGDVRAPL
                     DILTRLRLKRVGLSIDDFGTGHSSLAQLRDLPFDELKIDRGFVHGAADHASLAAILDS
                     SLRLARQLEMRSVGEGVEDRADWDHLAACGCDYAQGYFIGRPMPGERLVEWLHEWQAR
                     RTALAPAG"
     gene            2105..>4040
                     /locus_tag="PLS34_10600"
     CDS             2105..>4040
                     /locus_tag="PLS34_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP87949.1"
                     /translation="MLLRAGRWMMGKNRPLLIALLYAVVAVAWIFGSGMLLREVVADA
                     DTLHRIELAKGVLFVVVTSILLFLLMRRIESGRAARRQAEAAAALDFHLNNSPLAVVE
                     FDSELRILRWSGRAEEMFGWREDEMRGRRLDELDFVDPADKEAVLRTLRRLVDGKEQR
                     GFNLNRNLTRSGELRDCEWFNSVRRDEHGKVESVLSLALDVTARRRAERALTKSEAKF
                     RALVESAPDMIFVQADGRFTFVNRAGCRLLGATSAAQLVGQPILERVHPDWRELGQAR
                     MQALNERREPQPALEQVWLRLDGTPVDIEISGVPIRYGGQDGALVFARDIAERKTTAL
                     RLDDNARLLSMASTAAQIGGWSIDLQQQEVECSDEVCRIHDMSPQARVPVETWIGCYA
                     PEHRHRMREVLAACAADGVAFDEELKIVTTQGRRLWTRTIGQPVHDGAGRIVRIQGAF
                     QDISSRKHIERQLALQGQRAERLLELPGLADRLGETAFLQRALEIAIDLTGSRSGWLH
                     FVDADGGAPRLASSASFAGRGDREDDHPHAQEVALPIGEAVRLGRPVVSELRGTPAQP
                     SDGDSAGGDGPGWGVTVPARDGARVTMLMSVAGATKGEYSPFDLETLQLLADRVWGLI
                     ETRKIAAELERHRDHLEDLVA"
BASE COUNT          643 a         1367 c         1437 g          593 t
ORIGIN      
        1 gttcgccgac ggcggcgagt tcggcatggg cgcggagatc ggcatcgcca ccggccgcat
       61 ccacgcgcgc ggcccggtgg gcgcggagca gctgaccagc tacaagtacc aggtcagcgg
      121 cgcgggccag ctgcggccct gaagcccgcc gcctgcccgc ctgtgttatc gtatccgact
      181 cccctacacc atgattcccc ggaggaagga ccttgcgcaa cgcgcccctg gccgcgctcg
      241 cggcactgag ttttgttgtt ttcggagcgc ccctgcaggc gcaggaggat gccccagtga
      301 tccaggacgg cagcaccgtc agcattgaat acaccctgaa gctcgacgac ggctccacgg
      361 cggacaccaa cgccggccgc gagccgctgg tctacgtcca gggcgagcag cagatcctgc
      421 ccgcgctcga ggaaaagctg ctgggcatga aggccgacga gacccgcgaa gtcacgctga
      481 cgccggagga gggctacggc ccggtgcacg aggaggcgct gcagacggtg ccgctcgaca
      541 tcattcccga ggaagcccgc accgtcggcg ccaggctggt gggccagggg cccgagggcc
      601 agccgctgca cgcggtcgtg aaggagatca acgaggacag cgcggtcgtc gatctcaacc
      661 atccgctggc cggccagaag ctgcacttca gcatcaaggt cctgagcatc cagtagtctc
      721 cctcgcggcg gggcgctgcc ggccggctca tgccgtgccg gcagttgtcc tgccggagcc
      781 ggcccggtag attggtgccc ggatgcagtt caggcagggt cgtccggcga gccacagcgc
      841 acgccggacg ttgcctaacc gggagccccg atgaaggttc tgctgctgga cgacgatccg
      901 ttcgtactgc gcctgctgga gcggcaactc gccaatctag gcgtcgaagt ccggcacgcc
      961 tgcgaacgag ccgaactggc gctcgcgcaa ctggccgaag ggagcgccga cgtcgacgtg
     1021 gtgttctgcg acctgcagat gcccggcatg gacggcgtgg agttcctgcg caatctcgtc
     1081 gccatcgacg acccgcccgc gctggtgctg gtgagcggcg aggacacgcg cgtgctggag
     1141 agcgcggaac ggctggcgcg ggcccaccat ttggtcgtgc tcggggccct gcacaagccc
     1201 ttcgacatgg gacagctccg ccgggtgatc gaacgccacg aagcgctggc tcgcgacccg
     1261 acgctcaccg gcctgccgtc cgtgtactcg ccggaagagc tcggcgagtc gattcggcgc
     1321 ggcgaactcc tcaaccacta ccagcccaag gtcgagctgc gtaccggacg ggtggtgggc
     1381 gtcgaggcac tggtgcgctg gcagcattca cgcgacggcc tggtgtttcc ggatcgcttc
     1441 gtgccgatcg ccgaacagtc cggcctgatc gacgcgctgg gcagggccgt gctgggcaat
     1501 gccctcgcgc agctgcggca ctggcgcgac gcgggcctcg agctgcacgt cgcggtgaac
     1561 gtttccatgg ccagtctcgt caacctggcg ttcccggact acgtgctgcg ggaaaccgcg
     1621 gcggcaggag tgccggcgtc ggcgctggtg ctggaaatca ccgaaagccg cctgatggga
     1681 gacgtcaggg cgccgctcga catcctcacc cggctgcgcc tcaagcgggt cggcctgtcg
     1741 atcgacgact tcggcaccgg gcactcgtcg ctggcgcagc tgcgcgacct gcccttcgac
     1801 gaactgaaaa tcgatcgcgg cttcgtgcac ggcgcggcgg accatgcctc gctggcggcg
     1861 attctcgaca gcagcctgcg actcgcccgc cagctcgaaa tgcgcagcgt cggcgagggc
     1921 gtcgaggacc gcgcggactg ggaccacctg gccgcctgcg gctgcgacta cgcgcaggga
     1981 tatttcatcg gcaggccgat gccgggcgaa cgactggtcg aatggctgca cgaatggcag
     2041 gcacggcgca cggcactggc gcccgcggga tgaggccggg agccgcgcac agtcgggctt
     2101 cggggtgctg ctgcgcgcgg gacgctggat gatggggaag aaccggccgc tgctgatcgc
     2161 gctgctgtat gcagtggtgg ccgtcgcctg gattttcggc tccggaatgc tgctgcgcga
     2221 agtggttgcg gatgcggaca ccctgcatcg tatcgagctg gccaagggtg tgctgttcgt
     2281 cgtcgtcacg agcattctcc tgttcctgct catgcggcgc atcgagagcg gtcgcgcagc
     2341 ccgccgccag gccgaggccg cggccgcact cgacttccat ctcaacaatt cgccgctcgc
     2401 ggtggtcgag ttcgacagcg agctgcgcat cctgcgctgg tccggccgtg ccgaggaaat
     2461 gttcggctgg cgcgaggacg agatgcgcgg ccgcaggctc gatgagctgg atttcgtcga
     2521 tcccgccgac aaggaggccg tgctccgaac cctgcggcgc ctggtcgacg gcaaggaaca
     2581 gcgaggcttc aatctcaacc gcaacctgac ccggtcgggc gagctgcgcg attgcgagtg
     2641 gttcaactcc gtacgccgcg acgaacacgg caaagtcgaa tccgtgctgt cgctggcgct
     2701 ggacgtcact gcgcggcgcc gcgccgagcg cgcgctcacc aagagcgagg caaagttccg
     2761 cgccctggtc gaatcggcgc cggacatgat cttcgtgcag gcggacggcc gcttcacctt
     2821 cgtcaaccgc gccggctgcc ggctgctcgg cgccacctcg gccgcacagc tggtcggcca
     2881 gcccatcctc gagcgagtcc atccggactg gcgcgagctc gggcaggcgc gtatgcaggc
     2941 cctgaacgag cgacgcgagc cgcaaccggc cctggagcag gtctggctgc gactcgacgg
     3001 cacaccggtc gacatcgaga tctccggcgt gccgatccgc tatggtggcc aggacggcgc
     3061 gctggtgttc gcgcgcgaca ttgcggagcg caagaccacc gccctgcgcc tcgacgacaa
     3121 cgcacgcctg ctgagcatgg cgtcgacggc cgcgcagatc ggcggctgga gtatcgacct
     3181 gcagcagcag gaggtggagt gctcggacga ggtctgccgg atccacgaca tgtcgcccca
     3241 ggcccgcgtg ccggtggaga cgtggatcgg ctgctacgcg cccgagcatc ggcatcgcat
     3301 gcgcgaagtg ctggcggcct gcgccgcgga cggcgttgcc ttcgacgagg aactcaagat
     3361 cgtcacgacg caaggcagga ggctctggac ccgcaccatc ggccagccgg tgcatgacgg
     3421 cgccggccgc atcgtgcgca tccagggcgc cttccaggac atcagttcgc gcaagcacat
     3481 cgagcgacag ctggcgctgc agggccagcg cgcggagcgg ctgctcgagc tccccggcct
     3541 ggcagaccgc ctcggggaga cggcattcct gcagcgtgcg ctggagattg cgatcgacct
     3601 gaccggcagc cgatccggct ggctgcactt cgtcgacgca gacggcggcg caccgcgact
     3661 cgcatccagc gcctcgttcg ccgggcgcgg cgatcgcgag gacgatcacc cgcatgccca
     3721 agaggtggcc ctgccgatcg gcgaagccgt gcggctcggc cggccagtcg tcagcgagtt
     3781 gcgcggcacg ccggcgcaac cttcggatgg cgacagcgcg ggcggtgacg ggcctggctg
     3841 gggcgtcacc gtgccggcca gggacggcgc ccgggtgacg atgttgatga gcgttgcggg
     3901 cgcgacgaag ggcgagtact cgcccttcga cctcgagacg ctgcagctgc tggcggaccg
     3961 ggtctggggc ctgatcgaga ccaggaagat cgccgcggaa ctcgaacggc atcgcgacca
     4021 cctggaggac ctggtggcgc
//
LOCUS       DAOMPG010000216         4028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9260_length_4028_cov_4.967531, whole genome shotgun sequence.
ACCESSION   DAOMPG010000216 DAOMPG010000000
VERSION     DAOMPG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9260_length_4028_cov_4.967531"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLS34_10605"
     CDS             complement(<1..447)
                     /locus_tag="PLS34_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRP87950.1"
                     /translation="MTSPDRLAECGARVERSLERWLPGVDTAPQRLHEAMRYAVLGGG
                     KRIRPVLVYFTGQALGVPPGRLDGPAAAVELIHAYSLVHDDLPAMDDDDLRRGRPTCH
                     RAFDEATAILVGDALQVLAFEILTADPHMVPDPARRLEIMRLLARAS"
     gene            complement(444..710)
                     /locus_tag="PLS34_10610"
     CDS             complement(444..710)
                     /locus_tag="PLS34_10610"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRP87951.1"
                     /translation="MPKAKTPKSPAAEPVDELDFEQALAALEELVGRLESGDQPLERS
                     LADFERGVALTRRCQAALRDAEQKVEILSRDSAAGKLEPFEPEQ"
     gene            985..1407
                     /locus_tag="PLS34_10615"
     CDS             985..1407
                     /locus_tag="PLS34_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRP87952.1"
                     /translation="MSNLKGSKTEHNLKEAFSGESQANRRYLYFASKADVEGYNDVAT
                     VFRSTAEGETGHAHGHLEYLEAVGDPATGLPIGGTSDNLKAAIAGETHEYTDMYPGMA
                     KTARDEGFEEIADWFETLAKAERSHANRFQKALDNLDA"
     gene            1556..2896
                     /locus_tag="PLS34_10620"
     CDS             1556..2896
                     /locus_tag="PLS34_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRP87953.1"
                     /translation="MSVDSPRREGSLEAPTRHPVAWKSTEFWDEEKLFREMERVFDIC
                     HGCRRCFNLCNSFPTLFDAVDESDTGEVDGVAREVYWKVVDHCYLCDMCYMSKCPYVP
                     PHEWNVDFPHLMLRAKAVRFRKEGASRRDRILTSTDAVGRLAGIPVVAEVVNAVNQSS
                     GGRKLLESVLGVHRDAPLPKYHSKSMRSRLKGRPADRQGEPAEGTRGRVVLFATCYGN
                     RNTPEVGEDLAKVFEHNGIPVRLVEKEQCCGMPKMELGDLESVQRSMEANIPVLARAV
                     DEGWDIVAPVPSCVLMFKQELPLLFPDREDVKKVAAAMYDPFEYLSLRHRAGKLKTEF
                     KTTLGKVSYQVPCHLRVQNIGLKTRDILSLVQDTKVEAIERCSGHDGTYAVKKEFHEN
                     SVKIARPVVSRVKGATPDHFTSDCPMAGEQIAHGLKDGTTAQHPMTLLRIAYGL"
     gene            2947..3528
                     /locus_tag="PLS34_10625"
     CDS             2947..3528
                     /locus_tag="PLS34_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HRP87954.1"
                     /translation="MTQLTASDLMSLEQYARERASFRAKVLAHKKTRQVALGPNATLY
                     FEDGLTIQYQVQEMLRIERIFEAEGIEDELSAYNPLIPDGSNWKATFMLEYPDVELRK
                     RELARLVGVEDKVWVQVDGHDKVFAIADEDMERETEDKTSSVHFMRFELDQEMILALK
                     GGAGLAMGVDHDNLKVVVSVPEAVRDSLSNDLA"
     gene            3639..>4028
                     /locus_tag="PLS34_10630"
     CDS             3639..>4028
                     /locus_tag="PLS34_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP87955.1"
                     /translation="MSQDYNASSIEVLSGLDPVRRRPGMYTDTTRPNHLAQEVVDNSV
                     DEAIAGHCKRIAVTLFRDGSLQVEDDGRGMPVDVHPQEGIPGVELILTRLHAGGKFSQ
                     KNYRFSGGLHGVGVSVVNALSRNLEVWI"
BASE COUNT          718 a         1341 c         1365 g          604 t
ORIGIN      
        1 ggaggcgcgc gccagcagcc gcatgatctc cagccgccgc gccgggtccg gcaccatgtg
       61 cgggtcggcg gtgagaattt cgaaagcgag cacctgcagg gcatcgccca ccaggatcgc
      121 ggtggcctcg tcgaaggcgc gatggcaggt cggccggccg cgccgcaggt cgtcgtcgtc
      181 catggccggc aggtcgtcgt gcaccagcga gtaggcatgg atcagctcga ctgcagccgc
      241 cggtccgtcg aggcgacctg gcggcacgcc gagggcctgg ccagtgaagt acaccagcac
      301 gggacggatg cgcttgccgc cgcccagcac ggcgtagcgc atggcctcgt gcagtcgctg
      361 cggcgcggtg tcgacaccgg gcagccagcg ctccagcgag cgctcgaccc gcgcgccgca
      421 ctccgccagc cggtccgggc tcgtcactgt tcgggctcga acggttcgag tttcccggcg
      481 gcgctgtcgc gcgagaggat ttcgactttc tgctcggcgt cacgcaacgc ggcctggcag
      541 cggcgcgtca gcgccacgcc gcgctcgaag tccgccagcg accgttccag cggctgatcg
      601 ccggactcga gccggccgac gagttcctcg agagccgcca gcgcctgctc gaaatccagc
      661 tcgtccaccg gctcggcggc cggcgatttg ggggtcttgg ctttgggcat cgggactcgc
      721 gagatcggca acaagggccg cgctacgata ccggggcgcg cgccggcgaa gcaatccgca
      781 atgcccgcga gcagggtgtg gcgagttgac agcccggcac gcccggacta accttccagc
      841 attgatttag actaagtcta aattaagtcc agatcgcatt tgaggcgtgc ccgcagcggg
      901 catcgcgtcg ttggcggtca gtcagtttca cgacaccggg tccggtggca aaccgggctg
      961 aacaccatcc caggaggacg attcatgtct aacttgaagg gcagcaagac cgagcacaac
     1021 ctcaaggagg ctttctcggg cgagtcccag gccaaccgcc gttacctcta tttcgcgtcc
     1081 aaggccgacg tcgagggcta caacgacgtg gccaccgtgt tccgttccac tgcggaaggc
     1141 gagaccggcc atgcgcacgg ccacctcgag tacctcgagg cggtgggcga cccggccacc
     1201 ggcctgccga tcggcggcac ctccgacaac ctgaaggccg cgatcgccgg cgagacccac
     1261 gagtacacgg acatgtaccc gggcatggcg aagaccgccc gtgacgaggg tttcgaagag
     1321 atcgccgact ggttcgagac gctggccaag gcggagcgca gccacgcgaa ccgcttccag
     1381 aaggcgctgg acaacctcga cgcctgatgt cctttgcggg ggcgccgtcg gctgccggtc
     1441 gacggcgccc catgctgttt gggcatgctt ttggccagcg cgagcgcgcc gcgtcatcgc
     1501 ggcgtgaact ccccgcggct gcccggttgg aaccgaccac gaaggagcac tgctgatgtc
     1561 cgtcgattcg ccacgccgcg aaggcagcct ggaggcgccg acgcggcacc cggtcgcctg
     1621 gaagtccacg gagttctggg acgaggagaa gctgttcagg gagatggagc gcgtgttcga
     1681 catctgtcac gggtgtcgtc gctgcttcaa cctgtgcaat tccttcccca cgctgttcga
     1741 cgcggtcgac gagtcggaca ccggcgaggt ggacggcgtg gcgcgcgagg tctactggaa
     1801 ggtcgtcgac cactgctacc tctgcgacat gtgctacatg tcgaaatgcc cctacgtgcc
     1861 gccgcacgag tggaacgtcg atttcccgca cctgatgctg cgcgccaagg ccgtgcgctt
     1921 ccgcaaggaa ggcgccagtc gccgcgaccg catcctgacc tccaccgacg cggtcggccg
     1981 gctcgccggc atcccggtcg tcgccgaggt cgtcaacgcg gtcaaccaga gctcgggcgg
     2041 ccgcaagctc ctcgagagcg tgctcggcgt acatcgcgat gcgccgttgc cgaagtacca
     2101 ttccaagagc atgcgcagcc gcctgaaggg ccgcccggcc gaccgccagg gcgagcctgc
     2161 cgagggcacc cgcggccgcg tcgtgctgtt cgcgacctgc tacggcaacc gcaacacgcc
     2221 ggaggtgggc gaggacctgg cgaaggtgtt cgagcacaac ggcatcccgg tgcgcctggt
     2281 cgaaaaagaa caatgctgcg gcatgccgaa gatggagctg ggcgacctcg agtcggtaca
     2341 gcgctccatg gaagcgaaca tcccggtgct ggcgcgcgcc gtggacgagg gctgggacat
     2401 cgtcgcgccg gtgccgtcct gcgtgctgat gttcaagcag gagctgccgc tgctgttccc
     2461 cgaccgcgag gacgtgaaga aagtggcggc cgccatgtac gaccccttcg agtacctgtc
     2521 gctgcgccac cgggccggca agctcaagac cgagttcaag accacgctcg gcaaggtgag
     2581 ctaccaggtg ccgtgccacc tgcgcgtgca gaacatcggc ctgaagaccc gcgacatcct
     2641 ttcgctggtg caggacacca aggtcgaggc catcgagcgc tgttccggcc acgacggcac
     2701 ctatgcggtc aagaaggagt tccacgagaa ctcggtgaag atcgcgcgcc cggtggtcag
     2761 ccgggtcaag ggcgccacgc ccgaccattt caccagcgat tgcccgatgg cgggcgagca
     2821 gattgcgcac ggcctcaagg acggcaccac ggcgcaacac cccatgacgc tgctgcggat
     2881 cgcctacggg ctctgagggc gagttcgcgc acggcacacg gccatacgat tgacggagag
     2941 agacgaatga ctcagctgac tgcaagcgac ctgatgagcc tcgagcagta cgcgcgcgag
     3001 cgcgcgagct tccgggcgaa ggtgctggcg cacaagaaga cccgccaggt ggcgctgggc
     3061 ccgaacgcga cgctgtactt cgaggacggc ctgaccatcc agtaccaggt gcaggagatg
     3121 ctgcgcatcg agcgcatctt cgaggccgag ggcatcgagg acgagctgtc ggcctacaac
     3181 ccgctgatcc cggacggcag caactggaag gcgaccttca tgctggagta cccggacgtc
     3241 gaactgcgca agcgcgagct ggcgcgcctg gtcggcgtcg aggacaaggt ctgggtgcag
     3301 gtcgacggcc acgacaaggt gttcgccatc gccgacgagg acatggagcg cgagacggag
     3361 gacaagacgt cctcggtgca cttcatgcgc ttcgagctgg accaggaaat gatcctggcg
     3421 ctgaagggcg gcgccggcct cgccatgggc gtcgaccacg acaacctcaa ggtggtcgtc
     3481 tcggtgccgg aggcggtgcg cgactcgctg agcaacgatc tcgcctgaga aaatggggac
     3541 actccccaat tccgcttgcg gaattgggga gtgtccccat ttccatctgg tagaattccc
     3601 gccgcccggg tgctcttgcc cggcggtgca gggactccat gagccaggat tacaacgctt
     3661 cctcgatcga ggtcctcagc ggcctcgatc cggtccggcg caggccgggc atgtacaccg
     3721 acaccacgcg gcccaaccat ctcgcccagg aggtggtgga caacagcgtg gacgaagcca
     3781 tcgccggcca ctgcaagcgc atcgccgtga cgctgttccg cgacggctcg ctgcaggtgg
     3841 aggacgacgg ccgcggcatg ccggtcgacg tccatcccca ggaaggcatc ccgggcgtcg
     3901 agctgatcct cacccggctg cacgccggcg gcaagttctc gcagaagaac taccgcttct
     3961 cgggcggcct gcacggcgtc ggcgtgtccg tcgtgaacgc cttgtcacgc aacctcgagg
     4021 tgtggatc
//
LOCUS       DAOMPG010000217         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9363_length_4000_cov_3.945247, whole genome shotgun sequence.
ACCESSION   DAOMPG010000217 DAOMPG010000000
VERSION     DAOMPG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9363_length_4000_cov_3.945247"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLS34_10635"
     CDS             complement(<1..991)
                     /locus_tag="PLS34_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP87956.1"
                     /translation="MRPDSSLDEARQLIATGRSDDAVALLERNSAEPGCAVRLRELHL
                     GERRREQAMQVCMQMAGGYDAEAHVSRSVIALLQDDPERALREVRLALEVEPALATAH
                     NHLGRALHNSNQSLQAVSALRKAVELDDDYPEAWANLGLVLRALGGLDEAIKAYRKAV
                     ELAPGFITAELNLGISLLVAERVDEGLFCLQSVLKHAPQHQEALVNAGLALHLLGRFA
                     AARRHFEQALQLDPDDPLAWCYLGILLHEIHEPERALEALQRAVALNPMEIEAWVEVA
                     GIHEHAGRLDDAAAALERAMQVDPNDAAALLELARLRRRQGMLDEALALLGRAA"
     gene            1205..1618
                     /locus_tag="PLS34_10640"
     CDS             1205..1618
                     /locus_tag="PLS34_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRP87957.1"
                     /translation="MEKSAPEGGQRAASALASRLKSIDGFDFDEALEMALGRIEFLAK
                     MLGMFLATHADDAAGLRQLHADGDHAGVGRLAHGLKGAAATIGAGRLARLASEVQNAA
                     RERGKVSAGDVTGLVQESERFCAALRAALDPPRDA"
     gene            complement(1627..>4000)
                     /locus_tag="PLS34_10645"
     CDS             complement(1627..>4000)
                     /locus_tag="PLS34_10645"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRP87958.1"
                     /translation="ALFESAPGSYLVLTPGDYEIVAASEAYLRATMTTREQLTGKRLF
                     DVFPDDPERPDPEAAARLLASLERVTREKRADVLGVEFYPIQRPESLGGGFEDRYWSI
                     VHSPVPGPDGEVAFIIHRAEDVTEYLQSLPDHESTRQAFASHTEMMEADIVLRSRELD
                     DARRKLEQSQVLLRMASELAQLGAWTVELPDFDMTWSEGIYRIHEVPSGTPIELEAAM
                     SFYVPEHREAIRSAFQTCVEEGLPFDLELQIVNARGGRIWVRNIGQAVRDSSGQVIRV
                     QGALQDISVRREAEAKERELQDRLTGMLEHIGEGFMAVDRDWRVTYVNAEAVRMTGRD
                     RESLIGQVTWDLFPEAVGSQYEEQIIRAVREKVGVDFVEYFPPLDAWFEVRAYPSEEG
                     LATYFRDVTRERRLEEHIRQAQRLEAVGQLTGGVAHDFNNLLTVILGNAELLGEKLGT
                     DTPLGSLAVLISMAAQRGARLTNALLAFARRQPLEPVAVDVNELLRGLHDMLSRALGE
                     DIEIEFRMTEGLRAAMLDPAQFESAILNLCLNARDAMPGGGRLTIETGLRALDAEYVD
                     TRFGLAPGEYILVAVSDTGCGIPAEHLPRLFEPFFTTKEMGKGTGLGLPMVYGFVKQS
                     GGQVNVYSEVGEGTTIKLYLPPAEAEARAPHAEPPSEEPPRGTEKILLVEDDDMVRMY
                     AEDLLSGLGYEVTVAASGPAALEILEQGADVDLLFTDIVMPGGMNGRELAQRARGLRP
                     TLRVLYTSGYTENAVVHHGRLDPGVNLVSKPYRRAELAARVRAVLDRDPS"
BASE COUNT          622 a         1420 c         1311 g          647 t
ORIGIN      
        1 ccgctgcgcg cccgagcaac gccagcgcct cgtccagcat gccctgtctg cgccgcaggc
       61 gggccagctc gagcagggcc gccgcatcgt tcgggtcgac ctgcatggcg cgctccagcg
      121 ccgcggccgc gtcgtccagt cggccggcgt gctcgtgaat ccccgccact tcgacccagg
      181 cttcgatttc catggggttg agcgccaccg cgcgctgcaa ggcctccagt gcccgctcgg
      241 gctcgtggat ctcgtgcagc aggatgccca ggtaacacca ggcgagcgga tcgtccgggt
      301 ccagctgcag ggcctgctcg aaatgccggc gcgccgcggc gaagcggccc agcaggtgca
      361 gggccaggcc ggcgttgacc agcgcttcct ggtgctgcgg cgcgtgcttg agcaccgact
      421 gcaggcagaa caacccctcg tcgacccgct cggccaccag cagcgagata cccaggttga
      481 gttcggcggt gatgaagccg ggcgccagct ccacggcctt gcgataggcc ttgatggcct
      541 cgtccagccc gccgagggcg cgcagcacca ggccgagatt ggcccaggct tccgggtagt
      601 cgtcgtccag ttcgacggcc ttgcgcagcg ccgatacggc ctgcagcgac tggttggaat
      661 tgtgcagcgc ccggccgagg tggttgtgcg cggtcgcgag cgccggctcg acttcgagcg
      721 ccagccggac ctcgcgcagc gcgcgctcgg gatcgtcttg cagcagcgcg atcaccgagc
      781 ggctgacgtg ggcctcggcg tcgtacccgc cggccatctg catgcacacc tgcatcgcct
      841 gctcgcgccg ccgctcgccc aggtgcagct cgcgcagccg caccgcacag cccggttcgg
      901 cgctgttgcg ctcgagcagc gcgaccgcgt cgtcgctgcg accggtcgca atcaattgcc
      961 gggcttcgtc gagactggaa tctgggcgca tggtcggggt ccgtgccggt cagcggccgg
     1021 caagatctgg aggccagggg aacagactgg gcgggtgtcg aatccgacat cgtaccgaag
     1081 gccggccggc aggccaaccg gcggcccgga agcctgcgcc gacccccgcc gcaagggcca
     1141 tggcgtttgg tttacgcacc ggcgacggct ggtttaagct ggcccgccgc ccacaggtcg
     1201 acggatggag aaatcggcac ctgaaggagg ccagcgagcc gcgtccgcgc tggcttcgcg
     1261 ccttaaaagc atcgacggct tcgacttcga cgaggcgctg gagatggcgc tcggccgcat
     1321 cgagttcctg gcaaaaatgc tcggcatgtt ccttgccact cacgcggacg atgcagcagg
     1381 actacggcaa ctgcacgccg acggcgatca cgccggagtc ggccggctcg cacacgggct
     1441 caagggcgcc gccgccacca tcggcgccgg ccgcctcgcc cgcctggcct cggaggtgca
     1501 gaacgccgcc cgcgaacgag gcaaggtctc ggccggcgac gtgaccgggc tcgtgcagga
     1561 aagcgagcgg ttctgcgccg cactccgggc tgcgctggac ccgccgcgcg acgcctgatc
     1621 gttcgactac gacggatccc ggtccagcac cgcacggact cgtgcggcga gttcggcgcg
     1681 tcgataaggc ttcgacacca ggttgacgcc ggggtccagg cgcccgtgat ggaccactgc
     1741 gttctccgtg tagccggagg tatagagcac cctgagcgtg ggccgcaggc cccgggcgcg
     1801 ctgcgccagc tcacgaccgt tcatcccgcc cggcatgacg atgtccgtga acagcaggtc
     1861 gacgtcggcg ccctgctcga ggatttcgag ggcggccggt ccgctggctg ccaccgtgac
     1921 ctcgtagccg aggcccgaca gcaggtcctc tgcgtacatt cgtaccatgt cgtcgtcttc
     1981 gaccagcagg atcttctcgg tgccgcgcgg cggctcctcc gacggcggct cggcatgcgg
     2041 cgcgcgtgct tcggcctccg ccggcggcag gtacagcttg atggtggtgc cctcgcccac
     2101 ctccgagtag acgttgacct ggccgccgga ctgcttcacg aagccgtaga ccatcggcag
     2161 cccgaggccg gtgcccttgc ccatttcctt ggtggtgaaa aagggttcga acaggcgcgg
     2221 caggtgctcg gccggaatgc cgcaaccggt atcggacacg gcgaccagga tgtactcacc
     2281 cggtgcgagg ccgaagcggg tgtccacgta ctcggcgtcc agtgcgcgca ggccggtctc
     2341 gatggtcaat cgcccaccgc ccggcatcgc gtcgcgcgcg ttcaaacaga ggttcagtat
     2401 ggcgctctcg aactgggccg gatcgagcat tgcggcgcgc aggccctcgg tcatccggaa
     2461 ctcgatctcg atgtcctcgc cgagcgcgcg gctcagcata tcgtgcaacc cccgcagcag
     2521 ttcattgacg tccactgcga ccggctcgag cggctggcgc cgggcgaagg ccagcagcgc
     2581 gttggtcagt cgggcaccgc gttgagcggc catcgaaatc agcacggcga gcgacccgag
     2641 cggcgtgtcc gtgccgagct tttcaccgag caactcggcg ttgccgagga tcacggtgag
     2701 caggttgttg aaatcatgcg ccacgccgcc ggtcagctgg ccgaccgcct ccagtcgctg
     2761 tgcctggcga atgtgctcct ccagccgtcg ctcgcgggtg acgtcgcgga agtaggtcgc
     2821 caggccttcc tccgacgggt aggcgcggac ctcgaaccac gcgtcgagcg gcggaaaata
     2881 ctccacgaaa tcgacgccga ccttctcccg gaccgcgcgt atgatctgct cctcgtactg
     2941 cgagccgacc gcctctggaa acaagtccca ggtgacctgg ccgatcaggc tctcccggtc
     3001 gcggcccgtc atgcgcaccg cttcggcgtt cacataggtg acgcgccagt cgcggtccac
     3061 cgccatgaac ccttccccta tatgctccag catgccggtg agccggtcct gcaactccct
     3121 ctccttggcc tccgcttccc tgcgcaccga gatgtcctgc aaggcgccct gcacgcgaat
     3181 gacctgcccg ctgctgtcgc gtaccgcctg cccgatgttg cgcacccaga ttcgccctcc
     3241 ccttgcattg acgatttgca gctccagatc gaagggaagc ccctcttcca cgcaggtctg
     3301 gaatgcggat cgaatggctt cgcggtgctc ggggacatag aaactcatgg ccgcttcgag
     3361 ttcgatcggc gtccccgacg gcacctcgtg gatccggtag atcccctcgg accaggtcat
     3421 atcgaaatcc ggcagctcca cggtccaggc ccccagctgc gccagctctg acgccatgcg
     3481 caacaacacc tggctttgct ccagcttgcg cctggcatcg tcgagctccc ggctgcgcag
     3541 cacgatgtcc gcctccatca tctcagtgtg cgaggcaaat gcctggcggg tgctttcgtg
     3601 atccggcaaa gactgcaggt attcggtcac gtcctccgcg cgatgaatga tgaacgccac
     3661 ctcgccgtcg ggtcccggca cgggcgaatg cactatgctc cagtagcggt cctcgaagcc
     3721 gccgcccagc gattcgggcc gctgaatcgg atagaactcg acccccagca catccgcacg
     3781 tttctcccgg gtcactcgct ccagcgaggc cagcaggcgg gcagccgcct ccgggtccgg
     3841 ccgctccggg tcgtcgggaa agacatcgaa caggcgcttg cccgtcaact gttcccgggt
     3901 ggtcatcgtg gcccgcagat acgcctcgct ggcggcaacg atctcgtagt cgcccggcgt
     3961 gagcaccagg tagcttccgg gggccgactc gaacagcgct
//
LOCUS       DAOMPG010000218         3995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9378_length_3995_cov_2.678934, whole genome shotgun sequence.
ACCESSION   DAOMPG010000218 DAOMPG010000000
VERSION     DAOMPG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9378_length_3995_cov_2.678934"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLS34_10650"
     CDS             <1..1197
                     /locus_tag="PLS34_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRP87959.1"
                     /translation="GRRIIFVGDHDDWYRLYALPVDGGEPARLTGAYDVAGPRGAAWI
                     DVDPASGELLFVSAEHSPYERHVHRMAVQGGAARRVTSLPGVHQPSLSPDGRWLALLS
                     SNDTTPTELYLLDAEGEGVERRVTRSPLPEFYGHEWLAPRYVSFPSRTDDFLVHARII
                     EPPAREPGKRYPVILGSIYSNTVLNMWNPDRPTSILQQQMAMAGDYITVLVDVRGSIG
                     YGVAFREAFQGDWGGGDLEDLHAAVDYLAGLDHVDAERIGIWGNSYGGLLVLSALFKK
                     PGLFAAGVAGAPAVDIWHFGGFDQHLTRRPDTHPEIFARGSLMSLGEQLADPLLIIHG
                     LHDDIVPIKTTFMLTEKLALLGKPYELHIVHDSGHWWAASDHYARNTFGRLNDFLRRH
                     VPPGPR"
     gene            complement(1241..2446)
                     /locus_tag="PLS34_10655"
     CDS             complement(1241..2446)
                     /locus_tag="PLS34_10655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRP87960.1"
                     /translation="MKVLVLNSGSSTIKYGLYRMPAELPLAQGVIERIGEAEGRFLQR
                     DAEGERVEAMDVPDHAAGFAVLLERLAEHGAPDAIGHRVAHGGTRFSAAVRIDDAVLA
                     AIREMSPLAPLHNPANLAGIQAAMAARPGVPQVAVFDTAFHQSMPEHAWRFAVPEEWL
                     ARYGVRRYGFHGTSIRHVSRAAAQYLDRDPGELALVVLHLGNGASATAVQGGRSVDTS
                     MGLSALDGLVMGTRCGDLDPAVPGYLAARAGLAPAEIDRLLHEESGLKGLCGASDMRE
                     IERRVGAGDARAELALEVFCHRARRHVGALATSLGRLDALVFTGGIGEHQAGVRARIC
                     AGLEVVGVRLDATKNASPGALPAAVQAADSRAAVLVIATDEEREIARQVVDCLDAAGA
                     ETGVRHGFS"
     gene            complement(2443..>3995)
                     /gene="pta"
                     /locus_tag="PLS34_10660"
     CDS             complement(2443..>3995)
                     /gene="pta"
                     /locus_tag="PLS34_10660"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRP87961.1"
                     /translation="ELAAALGAARADDEPVFVIPEDAALSRPSVGDVGRELGARLLHG
                     TADSLRRDVGHVTVASMNLTHFLERLAPGSLVLTSGDRADILLGSFVANRSAAASGLA
                     GIILTGGLSPAVKIRHLISGLSDESLPVLAVDTDTYEAATQVRDVRAWLSAENESKVA
                     RALGLFEARVGAAALEARIELARPTRMTPLMFEYELMSRAARLRKRIVLPEGEEERIL
                     RAADILVKRGTAEIVLLGDETLIRRRISALALDLGNVEIIDPSQAPQLEAYAAELHRL
                     RKHRGVTPERALDMMRDRSYFGTMMVHMGHVDAMVSGAVTTTAHTIRPAFEFIRTRPD
                     VSVASSVLFMCMRDRVLVYADCAVVPEPTAEELADIAISSATTAQMFGIEPRIAMLSY
                     STGESGAGAEVERVRAATARVRARRPDLPVEGPIQYDAAVDAAVARTKIPGSEVAGRA
                     TVLVFPDLNSGNNAYKAVQRSGGAVVVGPVLQGLNKPVNDLSRGASVGDIVKTVAITA
                     VQAQGLPW"
BASE COUNT          579 a         1467 c         1376 g          573 t
ORIGIN      
        1 ggaaggcgga tcattttcgt cggcgaccac gacgactggt accgcctgta tgccctgccg
       61 gtcgacggcg gtgagcctgc ccgcctgacc ggtgcctatg acgtggccgg gccgcgcggc
      121 gccgcctgga tcgacgtcga cccggccagc ggcgagctgc tgttcgtctc ggccgagcac
      181 agtccctacg agcggcatgt ccatcgcatg gcggtccaag gcggcgccgc aaggcgcgtc
      241 acttccctgc ccggcgtcca ccagccgtca ctttctcccg acgggcgctg gctggccctg
      301 ctgagctcga acgacacgac gcccacggaa ctgtacctgc tggacgccga gggcgaaggc
      361 gtggagcgcc gcgtcacgcg ctcgccgctg ccggagttct acggccacga gtggctggcg
      421 ccacgctacg tgagtttccc gagccgcaca gacgacttcc tggttcacgc ccgcatcatc
      481 gagccgccgg ctcgcgagcc cggcaagcgc tacccggtga tcctcggcag catctattcc
      541 aacaccgtgc tgaacatgtg gaacccggat cggccgactt ccattctcca gcagcagatg
      601 gccatggcgg gcgactacat cacggtgctg gtggacgtgc gcggcagcat cggctacggc
      661 gtggcgtttc gcgaggcctt ccagggcgac tggggcggcg gcgacctgga ggacctgcac
      721 gccgccgtcg attacctggc gggactggac cacgtcgacg ccgagcgcat cggcatctgg
      781 ggcaacagct acggcgggct gctggtgctg tccgcgctgt tcaagaaacc gggcctgttc
      841 gccgccggcg tggccggcgc accggccgtc gacatctggc atttcggcgg cttcgatcag
      901 cacctgacgc gccggcccga tacgcatccg gagattttcg cgcgcggcag cctgatgagc
      961 ctgggcgaac agctcgccga cccgctgctc atcatccacg gcctgcacga cgacatcgtg
     1021 ccgatcaaga cgaccttcat gctgaccgag aagctggcac tgctgggcaa gccctatgag
     1081 ctgcacatcg tgcacgactc ggggcactgg tgggcggcct cagatcacta cgcccgcaac
     1141 actttcggcc ggctcaacga cttcctgcgc cgccacgtgc ccccgggccc acgctgaaaa
     1201 ccggggtcgg caccgaaacc ggggtcagac acgggttttt ttaagaaaac ccgtgtctga
     1261 ccccggtttc ggcgccagcc gcgtcgaggc agtcgacgac ctggcgcgcg atctcgcgct
     1321 cctcgtcggt ggcgatcacc agcacggccg cccggctgtc ggcggcctgg accgcggccg
     1381 gcaacgcgcc cggcgacgcg ttcttcgtcg cgtccagccg gacgccgacc acctcgagcc
     1441 cggcgcagat gcgggcgcgc acaccggcct ggtgctcgcc gatgccgccg gtgaacacca
     1501 gcgcatccag ccgcccgagg ctggtcgcca gcgcgccgac atgccggcgc gcgcggtggc
     1561 agaagacctc caaggccagc tcagcgcgcg cgtcgcctgc gccgacgcgg cgctcgatct
     1621 cgcgcatgtc gctggcgccg cacagcccct tgagaccgct ttcctcgtgc agcagccggt
     1681 cgatctcggc gggcgccagg ccggcgcgtg cggccaggta gccgggcacc gccggatcga
     1741 ggtcgccgca gcgggtgccc attaccagcc cgtccagcgc cgacaggccc atcgaggtgt
     1801 cgacgctgcg cccgccctgc accgccgtcg cgctggcgcc gttgccgagg tgcagcacga
     1861 ccagcgcaag ctcgccgggg tcccggtcga ggtattgcgc agccgcgcga ctgacatggc
     1921 ggatggaggt gccgtgaaat ccgtagcgcc gcacgccgta gcgggccagc cactcctccg
     1981 ggaccgcgaa gcgccaggcg tgttccggca tcgactggtg gaacgcagta tcgaacaccg
     2041 cgacctgcgg aacgcccggg cgcgccgcca tcgcggcctg gatgcccgcc aggttggccg
     2101 ggttgtgcag cggcgccagc ggactcatct cgcgaatcgc cgccagcacc gcatcgtcga
     2161 tgcgcactgc ggcgctgaag cgcgtgccgc cgtgcgccac gcgatgcccg atggcatccg
     2221 gcgcgccgtg ctccgccagc cgttcgagca gcaccgcgaa gcccgcggca tggtccggca
     2281 cgtccatcgc ctcgacgcgc tcgccctcgg catcgcgctg caggaagcgc ccctcggcct
     2341 cgccgatgcg ctcgatgaca ccctgtgcga gcggaagctc cgcgggcatg cggtacagcc
     2401 cgtacttgat ggtcgaactg ccggaattga gcaccagcac tttcaccagg ggagcccctg
     2461 cgcctgcacc gccgtgatcg ccaccgtctt gacgatgtcg ccgacgctgg cgccgcgcga
     2521 caggtcgttg accggcttgt tgagcccctg cagcaccggc ccgaccacca ccgcgccgcc
     2581 cgagcgctgc accgccttgt aggcgttgtt gccggaattg aggtcgggga acaccagcac
     2641 cgtggcgcgg ccggcgacct cgctgcccgg gatcttggtg cgcgccaccg cggcgtccac
     2701 ggcggcgtcg tactggatcg gcccctcgac cggcaggtcg gggcgccgcg cgcgcacccg
     2761 cgctgtcgcg gcgcggacgc gctcgacctc ggcgccggca ccggattcgc cggtggaata
     2821 ggacagcatg gcgatgcgcg gctcgatgcc gaacatctgc gccgtggtcg ccgagctgat
     2881 cgcgatgtcg gccagctctt ccgccgtcgg ttcgggcacc actgcgcagt ccgcatacac
     2941 gagcacgcgg tcgcgcatgc acatgaatag cacgctggag gcgaccgaga cgtcgggccg
     3001 cgtgcggatg aactcgaagg ccgggcggat ggtgtgcgcc gtggtcgtca ccgcgcccga
     3061 caccatcgcg tccacgtggc ccatgtgcac catcatcgtg ccgaagtagc tgcggtcgcg
     3121 catcatgtcg agcgcacgct ccggcgtgac gccgcgatgc ttgcgcagcc ggtgcagctc
     3181 cgccgcgtag gcctcgagct gcggcgcctg cgaaggatcg atgatctcga cgttgcccag
     3241 atcgagcgcc agcgccgaga tgcgccgccg gatcagggtc tcgtcgccga gcagcacgat
     3301 ctcggccgtg ccgcgcttca ccaggatgtc cgccgcgcgc aggatgcgtt cctcctcgcc
     3361 ctccggcagc acgatgcgct tgcgcagtcg cgccgcgcgg ctcatgagct cgtattcgaa
     3421 catcagcggg gtcatgcgcg tcggccgcgc cagctcgatg cgcgcctcga gcgccgcggc
     3481 gccgacccgc gcctcgaaca ggccgagtgc gcgcgccacc ttggactcgt tctccgcgct
     3541 cagccaggca cgcacgtcgc gcacctgcgt ggcggcctcg taggtgtcgg tgtccaccgc
     3601 gagcaccggc agcgactcgt ccgacaggcc gctgatgaga tggcggattt tcaccgccgg
     3661 cgacaggccg ccggtgagga tgatgccggc caggccgctg gcggccgccg agcggttggc
     3721 gacgaagctg ccgagcagga tgtcggcgcg gtcgcccgag gtgagcacca ggctgccggg
     3781 cgcaaggcgc tccaggaagt gagtcaggtt catcgatgcc acggtgacgt gcccgacgtc
     3841 gcggcgcagc gagtcagccg tgccgtgcag cagccgggcg ccgagctcgc ggccgacgtc
     3901 gccgaccgac ggccggctga gcgcggcgtc ctcgggaatg acgaacaccg gctcgtcgtc
     3961 ggcgcgggcc gcgccgaggg ccgccgccag ctcgt
//
LOCUS       DAOMPG010000219         3983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9412_length_3983_cov_5.113289, whole genome shotgun sequence.
ACCESSION   DAOMPG010000219 DAOMPG010000000
VERSION     DAOMPG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_3983_cov_5.113289"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..573)
                     /gene="nrdR"
                     /locus_tag="PLS34_10665"
     CDS             complement(73..573)
                     /gene="nrdR"
                     /locus_tag="PLS34_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRP87962.1"
                     /translation="MYCPFCRHEETKVIDSRLAGNGLQIRRRRQCLACDERFTTFETA
                     ELVLPRLVKRDDRREPFDESKLRAGVMRALEKRPVAAEAVEEAISRILQRLLTAGERE
                     LPSSYVGQLVMDELRQLDEVAYVRFASVYRSFQDVEEFRREIERLRDVAPVGREQMSL
                     LPREGK"
     gene            complement(609..1862)
                     /gene="glyA"
                     /locus_tag="PLS34_10670"
     CDS             complement(609..1862)
                     /gene="glyA"
                     /locus_tag="PLS34_10670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRP87963.1"
                     /translation="MFSPEMKIEGFDDELFAALQAERRRQEEHVELIASENYASPRVL
                     EAQGSVLTNKYAEGYPGRRYYGGCEYVDQAEELAVERAKQLFGADYVNVQPHSGSQAN
                     VAAFMAMIEPGDTLLGMSLAHGGHLTHGAKVNFSGRVYHAVQYGLTADGNEIDYDQVA
                     ALAKEHRPKLIIAGFSAYSRVVDWRRFREIADSVGALLMADMAHVAGLVAAGVYPSPV
                     PYADVVTSTTHKTLRGPRGGIIMAKHNEALEKKLNSLVFPGTQGGPLMHVIAAKAVAF
                     KEALQPEFKAYQQQVVANARAMAATLVERGYRIVSGGTDNHLFLVDLIDKDITGKDAD
                     AALGRANITVNKNSVPNDPRSPFVTSGLRMGTPAVTTRGFGEAECRTLAGWIADVLDN
                     IEDEATIRRVRDQVLGLCGRFPVYG"
     gene            complement(2435..>2759)
                     /locus_tag="PLS34_10675"
     CDS             complement(2435..>2759)
                     /locus_tag="PLS34_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HRP87964.1"
                     /translation="GCKLNIPLHPGADDQEFFAAWQECEAFLQDARPEFYIFVAGADS
                     LAGDPLTHLQYSEDAHGSAARAICRLADEHAKGRVLGLGAGGYNRRNVAAAWSRVVAE
                     FADSV"
     assembly_gap    2760..2769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2770..3410)
                     /locus_tag="PLS34_10680"
     CDS             complement(<2770..3410)
                     /locus_tag="PLS34_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HRP87965.1"
                     /translation="MFLKRRKKPEVRILAGESIAEYGFGDGHPFGRDRHDVFLRSLRE
                     RGIEERVWIDDPERPASRAELELFHTPRHVRFIERMSQEGIGFLDEGDTPAFPGVYHS
                     AANVVGATLQAGELLVSGAARRVFIPIAGLHHAARDKTSGFCVFNDIGVLVENLRKQH
                     GIRRIAYVDIDAHHGDGVYYGFASDPDLVFVDIHEDGRFLFPGTGHREETGIGP"
     gene            3602..>3983
                     /locus_tag="PLS34_10685"
     CDS             3602..>3983
                     /locus_tag="PLS34_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP87966.1"
                     /translation="MAQYIYTMNRVAKVVPPKRYILRDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGRDTEIEGEARPQPGIRIGYLEQEPQLDPAKDVRGNVEDALGDVKGAQA
                     RLDEIYAAYAEPDADFDALAKEQAE"
BASE COUNT          608 a         1391 c         1353 g          621 t
ORIGIN      
        1 gtccccggcg ggcgagctcc agcgcccgcc ccatccagcg gcggtccggc gcgctgaatt
       61 ccgccgcggc gctcacttgc cctcgcgcgg cagcaacgac atctgctcgc ggccgaccgg
      121 cgcgacgtcg cgcaagcgct cgatctcgcg gcggaactcc tcgacgtcct ggaagctgcg
      181 gtacacggag gcgaagcgca cataggccac ctcgtcgagc tgtcgcagct catccatcac
      241 cagctggccg acgtagctcg agggcagctc ccgctcgccg gcagtcagca ggcgctgcag
      301 gatccgggag atggcctcct cgaccgcctc cgccgccacg gggcgcttct ccagcgcgcg
      361 catcacgccg gcgcgcagct tgctctcgtc gaagggctcg cggcggtcgt cacgcttgac
      421 cagccggggc agcaccagct cggcagtctc gaaggtcgtg aagcgctcgt cgcaggccag
      481 gcactggcgg cggcgccgga tctgcagccc gttgcccgcc agccgcgagt cgatcacctt
      541 ggtctcctcg tggcggcaga aaggacagta catggcgttt ccggacggcg ctcagccccg
      601 gcagggcctc agccgtagac gggaaagcgg ccgcacagcc cgagcacctg gtcgcgcacc
      661 cgccggatgg tcgcttcgtc ctcgatatta tcgagcacgt cggcgatcca gcctgcgagg
      721 gtgcggcact cggcctcgcc gaagccgcgc gtggtgaccg ccggggtgcc catgcgcagg
      781 ccgctggtga cgaagggcga gcgcggatcg ttgggcaccg agttcttgtt cacggtgatg
      841 ttggcgcgcc cgagcgctgc gtccgcgtcc ttgccggtga tgtccttgtc gatcaggtcg
      901 acgaggaaca ggtggttgtc ggtgccgccg gacacgatcc ggtagccgcg ctccaccagc
      961 gtcgccgcca tggcgcgggc gttggccacg acctgctgct ggtaggcctt gaactccggc
     1021 tgcagcgcct ccttgaaggc caccgccttg gcggcgatga cgtgcatcag cggcccgccc
     1081 tgggtgcccg ggaacaccag cgaattgagc ttcttctcca gcgcttcatt gtgtttcgcc
     1141 atgatgatgc cgccgcgcgg cccccgcagc gtcttgtggg tggtcgaggt gacgacgtcg
     1201 gcgtagggca cggggctcgg gtacacgccc gccgccacca ggccggcgac gtgcgccatg
     1261 tcggccatca gcaaggcgcc gaccgagtcg gcgatctcgc gaaaacgccg ccagtccacc
     1321 acgcgcgagt aggcggagaa gccggcgatg atgagcttcg gccggtgctc cttcgccagc
     1381 gccgcgacct ggtcgtagtc gatctcgttg ccgtcggcgg tcagcccgta ctgcacggca
     1441 tgatagaccc ggccggagaa gttcaccttg gcgccgtggg tgaggtggcc gccgtgcgcc
     1501 aggctcatgc cgaggagggt gtcgcccggc tcgatcatcg ccatgaacgc agccacgttc
     1561 gcctgcgacc ccgaatgcgg ctgcacgttg acgtaatcgg cgccgaacaa ctgcttggcg
     1621 cgctcgaccg ccagctcctc ggcctggtcg acgtactcgc agccgccgta gtagcggcgc
     1681 ccgggatagc cctcggcgta cttgttggtc agcaccgagc cctgggcctc gagcacccgc
     1741 ggactggcgt agttttccga cgcgatcagc tcgacgtgct cctcctggcg ccgccgctcc
     1801 gcctgcagcg ccgcgaacag ttcgtcgtca aagccttcaa tcttcatctc ggggctgaac
     1861 atcggcaggg atctccgggg gcgagaggcg aaaacggcat tttaccccac cggcgcaaaa
     1921 ccggggtcag acacccgttt ccgattgccg gcagccgccg cagattttcg cgctttgttc
     1981 cagttgcccg cgggcgcgca gcgcctttgg ttccaatcgt ccgcgagcgc ggggccttcg
     2041 gttccggctg tccgcgggcg cgcggcgcca taggccggcc tgcgctcgga caaggaaaaa
     2101 ctcgctcggc cggcctatga caccccacac ccgctacgac cgcgactaca gccacgcccc
     2161 gcctttgtgg gaacgctgat cattgccaag gtcggccagt tcccgcgcaa cagcgccgag
     2221 ccgggcaatg gctggccagc accaaggcgg cctcgccagg ccgggcagcc gttgacgcca
     2281 agcctttcga cgcggcagcg ggggtggggc gtcatgggcg cgctgagtga gcttggattt
     2341 cgccgagcga agcgcgccca tggcggcgca ccgccgcggt cgaccggaaa ctggtgtctg
     2401 accccggttg ttggaaaagg gtgtctgacc ccggttatac gctgtcggca aactcggcga
     2461 ccacgcgact ccaggcggcg gccacgttgc ggcggttgta gccgccggcg cccagcccga
     2521 gcacccggcc cttggcgtgc tcgtccgcca ggcggcatat cgcccgggcg gcggagccgt
     2581 gggcatcctc gctgtactgc aggtgagtca gcggatcgcc ggccaggctg tccgccccgg
     2641 ccacgaatat gtagaactcc ggccgggcgt cctggaggaa ggcctcgcac tcctgccagg
     2701 cggcaaagaa ttcctggtcg tccgcgccgg gatgcagcgg gatgttgagc ttgcaacccn
     2761 nnnnnnnnng ggccgatgcc cgtctcctcg cggtggccgg tgccggggaa caggaagcgg
     2821 ccgtcctcgt ggatgtccac gaacaccagg tccgggtccg aggcgaagcc gtagtacacg
     2881 ccgtcgccgt gatgggcgtc gatgtccacg taggcgatgc gccggatgcc gtgctgtttg
     2941 cggaggtttt ccaccagcac gccgatgtcg ttgaatacgc agaagcccga cgtcttgtcg
     3001 cgggccgcgt ggtgcaggcc ggcgatcggg atgaacaccc ggcgcgccgc cccgctgacc
     3061 agcagctcac ctgcctgcag cgtcgcgccg accacgttgg cggccgagtg gtagacgccg
     3121 gggaaggccg gcgtgtcgcc ctcgtcgagg aagccgatgc cctcctgaga catgcgctcg
     3181 atgaagcgga cgtggcgcgg cgtgtggaac agctcgagtt cggcgcggct ggcgggccgc
     3241 tcggggtcat cgatccagac ccgctcctcg atgccgcgct cgcgcaggct gcgcaaaaac
     3301 acgtcgtgcc ggtcacggcc gaacgggtgg ccgtcgccaa agccgtattc ggcgatggac
     3361 tcgcccgcga gaatccgcac ctcgggtttc ttccggcgct tcaggaacac ccggccgctc
     3421 cgctcgagaa ggtttgctgc cagcataaac cagcgcccgg cagttgacac gcggcatttc
     3481 ggggcgccga cgcggcctgc tcccgctgcc tgtgacggcc cttgccgtcc ggcccgattt
     3541 cccgccacgg ccggcgtgct tcataatgcc gcgccctaga cccacctcca gaacgagaac
     3601 catggcgcag tacatctaca cgatgaaccg cgtcgccaag gtcgtgccgc cgaagcggta
     3661 catcctgcgc gacatttccc tgtccttttt tccgggcgcc aagatcggcg tgctcggcct
     3721 gaatggctcg ggcaagtcca ccctgctcag gatcatggcc ggccgcgaca ccgagatcga
     3781 gggcgaagcc aggccgcagc cgggtatccg catcggctac ctcgagcagg aaccgcaact
     3841 cgacccggcc aaggacgtgc gtggcaacgt cgaggatgcg ctcggcgacg tcaagggcgc
     3901 gcaggcgcgg ctggacgaga tctacgccgc gtacgccgag ccggacgccg acttcgatgc
     3961 gctggcgaag gaacaggcgg agc
//
LOCUS       DAOMPG010000220         3956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9493_length_3956_cov_4.775955, whole genome shotgun sequence.
ACCESSION   DAOMPG010000220 DAOMPG010000000
VERSION     DAOMPG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9493_length_3956_cov_4.775955"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLS34_10690"
     CDS             complement(<1..868)
                     /locus_tag="PLS34_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP87967.1"
                     /translation="MERYLPAEQVKQVMGAYEFAAKAHEGQQRQSGEPYISHPVAVAT
                     LLAELKLDAQTLMAALLHDVIEDTGVPKEDIAGMFGEEVAELVDGVSKLDKIEFRSRA
                     EAQAESFRKMLLAMVGDMRVILVKLADRTHNMRTLWIMPPDKRRRIARETLEIYAPIA
                     NRLGMNQIRLELEDLGFRCLHPYRYRVLERALKRAQGSQKQILQKISKRFGANLEEAG
                     IEARVAGREKHIYSIYRKMRDKKRALNEIVDVYGIRIVVDSVDDCYRALGIVHKCYKP
                     MPGRFKDYIAIPR"
     gene            complement(965..1228)
                     /gene="rpoZ"
                     /locus_tag="PLS34_10695"
     CDS             complement(965..1228)
                     /gene="rpoZ"
                     /locus_tag="PLS34_10695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRP87968.1"
                     /translation="MARITVEDCMQNVDNLFDLVLIAARRARQLANGAPSSLEWQNDK
                     ATVVALREIAGGAIGHDILNEPDPPIAVREPEPFDRPIPPMGD"
     gene            complement(1331..1948)
                     /gene="gmk"
                     /locus_tag="PLS34_10700"
     CDS             complement(1331..1948)
                     /gene="gmk"
                     /locus_tag="PLS34_10700"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRP87969.1"
                     /translation="MSAQNRLFVISAPSGAGKTSLVRALMEREPDLRFSISYTTRAKR
                     PGEHDGQHYFFVTADAFRRMIEAGEFLEHARVFDNYYGTARAQVEALLADGHGVVLEI
                     DWQGARQVRDAMPDSVGIFILPPSRAELERRLRGRATDSEEVIRRRLADAATDMAHWN
                     EFQYVVVNDDFATALGALADIVRGRGAASRADRPELGPVIGALLD"
     gene            complement(1957..2829)
                     /locus_tag="PLS34_10705"
     CDS             complement(1957..2829)
                     /locus_tag="PLS34_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRP87970.1"
                     /translation="MIRSMTGFARAEAGAPWGSVSWELRSVNHRYLETALRLPEELRP
                     LEARFRQLIAAGLKRGKVDGTLRVSWESAGRGELAVDLELAERVAEAAARIRARVGEL
                     ALPGTIDLLRWPGVVREQERDLEPVADLAAGALEQALGDLDAARSREGGRLAAALAER
                     CEGIDALAEQVRGALPAIRTGLRARLLERLAGLDLEADPQRLEQELAIQLGKMDVDEE
                     LDRLGSHIAEVRRILATPDDEAQGRRLDFLMQEFNREANTLGSKSVDSETTRIAVELK
                     VLIEQMREQVQNIE"
     gene            2940..3659
                     /locus_tag="PLS34_10710"
     CDS             2940..3659
                     /locus_tag="PLS34_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRP87971.1"
                     /translation="MSLLGDRDENQDRAAIHRNNGSVLLVVADGMGGHSGGALAAEVA
                     VDSLCRAFAQAAPEQDGRSFLQGAVEAAHDAVVAIGEKVGVGSRPRATCAVCLVRDDH
                     ATWAHVGDSRVYLARAGAVVTRTRDHTPIESLLQDGLISEEEIAGHPMRHYVEYCLGG
                     LAERPLITISEPVPLAADDLVVVCSDGFWSGLADADIGAGPEQGAGLQDWLSRLAGRA
                     VRVGTPYSDNTTVVALRALAA"
     gene            3679..>3956
                     /gene="rph"
                     /locus_tag="PLS34_10715"
     CDS             3679..>3956
                     /gene="rph"
                     /locus_tag="PLS34_10715"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223089.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRP87972.1"
                     /translation="MRPSQRRADELRAVRFERGFQRHAEGSVLVEFGATRVLCAASVE
                     DRVPPFLKGKGKGWVTAEYGMLPRATHTRNPREAARGRQGGRTLEIQRL"
BASE COUNT          564 a         1358 c         1377 g          657 t
ORIGIN      
        1 cgcgcggaat ggcgatgtag tccttgaagc gccccggcat cggcttgtag cacttgtgca
       61 cgatgcccag cgcccggtag cagtcgtcca cgctgtcgac cacgatgcgg atgccgtaca
      121 cgtccacgat ctcgttcagt gcgcgcttct tgtcgcgcat cttccggtag atgctgtaaa
      181 tgtgtttctc gcgcccggcg acccgtgctt cgatgccggc ctcctcgaga ttcgcgccga
      241 agcgcttgct gattttctgc aggatctgct tctggctgcc ctgtgcgcgc ttcaatgcgc
      301 gctcgaggac gcgatagcga tacgggtgca ggcagcggaa gccgagatcc tcgagctcca
      361 ggcggatctg gttcatgccg agccggttgg cgatcggcgc gtagatctcg agggtttcgc
      421 gcgcgatccg gcggcgcttg tccggcggca tgatccacag cgtgcgcatg ttgtgggtgc
      481 ggtcggcgag cttcacgagg atgacacgca tgtcgccgac catggcgagc agcatcttgc
      541 ggaagctttc cgcctgcgcc tcggcgcggc tgcggaactc gatcttgtcc agcttgctga
      601 ccccgtcgac cagttcggcg acctcttcgc cgaacatgcc ggcgatgtcc tccttcggca
      661 cgccggtgtc ctcgatgacg tcgtgcagca gcgccgccat gagggtctgg gcatcgagtt
      721 tcagctcggc cagcagcgtg gcgaccgcca cggggtggga gatgtagggc tcgccggact
      781 ggcgctgctg gccctcgtgc gccttggcgg cgaactcgta ggcgcccatg acctgcttga
      841 cctgctccgc cggcaggtag cgctccatcc ggcgggtcag gcggtcaagc ccaatcgacc
      901 gcttcctttt cggcagtcga gccaggaagg aagcggcgta gtccacgggt gcgatcccct
      961 ggcgtcagtc tcccatcggg gggatgggac ggtcgaaggg ctccggctca cgcacggcga
     1021 tcggcggatc cggctcgttg aggatgtcgt ggccgatcgc gccgccggcg atctcccgca
     1081 gcgcgaccac cgtggccttg tcgttctgcc actccaggct ggacggcgcg ccgttggcga
     1141 gctgtcgcgc gcgacgagcg gcgatcagga cgaggtcgaa caggttgtcg acgttctgca
     1201 tgcaatcttc gacggtgatg cgggccatgt gactctctgt gctaaaggac gccccgggct
     1261 ccggggccag cgcttcatgt taacggatta gcggccggcg ctgccagtac gatgccggct
     1321 ggtcggcgct tcaatcgagc agcgcgccga tcaccggccc gagctccggg cggtccgccc
     1381 ggctggcggc gccccggccg cggacaatat ccgccagcgc ccccagggca gtcgcgaaat
     1441 cgtcgttgac gacgacgtac tggaactcgt tccagtgcgc catgtccgtg gccgcgtccg
     1501 ccaggcgacg gcggatgacc tcctcgctgt cggtcgcccg gccgcgcagc cgccgctcaa
     1561 gctcggcgcg cgacggcggc aggatgaaga tacccaccga atccggcatg gcatcgcgca
     1621 cctgccgggc gccctgccag tcgatttcga gcaccacgcc gtggccgtcg gccagcagtg
     1681 cctccacctg ggcgcgggcg gtgccgtagt agttgtcgaa caccctggcg tgttccagga
     1741 actccccggc ctcgatcatc cgccggaagg cgtcggccgt gacgaagaaa taatgctggc
     1801 cgtcatgctc tcccggccgc ttcgcgcgcg tggtgtacga gatggagaaa cgcaggtcgg
     1861 gctcgcgctc catcagcgcc cgcaccaggc tggtcttgcc ggcaccggac ggcgcagaga
     1921 tgacgaacag ccggttctgt gcgctcaagt ccggcctcac tcgatgttct ggacctgctc
     1981 gcgcatctgc tcgatcagca ccttgagctc aacggcgatg cgggtggtct cgctgtctac
     2041 ggacttggag ccgagcgtgt tggcctcccg gttgaattcc tgcatcagga aatcgagccg
     2101 ccgcccctgc gcctcgtcgt ccggtgtcgc caggatgcgg cgcacctccg cgatgtggct
     2161 gccgaggcgg tcgagttcct cgtcgacgtc catcttgccg agctggatcg cgagctcctg
     2221 ttcgaggcgc tgtggatcgg cttcaaggtc gagtcctgcg aggcgttcga gcaatcgtgc
     2281 acgcaacccc gtgcgaatgg cgggcaacgc cccgcgcacc tgttcggcca gtgcgtcgat
     2341 gccttcgcat cgctccgcca gcgccgcggc gagccgcccg ccctcgcggc tgcgggccgc
     2401 atcgagatct cccagcgcct gctcgagggc gccggcggcc aggtcggcga ccggttcgag
     2461 gtcgcgctcc tgttcccgga ccacgcccgg ccagcgcagc aggtcgatgg tgccgggcag
     2521 ggccagctcg ccgacccgcg cacggatcct ggccgcggcc tcggcgacgc gctcggccag
     2581 ttccaggtct actgccagct cgccacgtcc ggccgactcc cagctgacgc gcagcgtgcc
     2641 gtccaccttg ccgcgcttca ggccggccgc gatcagctgg cggaaccggg cctcgagggg
     2701 acgcagctct tcgggcaggc gcagggcggt ctcgaggtag cgatggttga cggaacgcag
     2761 ttcccaggag acggatcccc agggcgcgcc ggcctcggcg cgggcgaacc ccgtcatgct
     2821 ccggatcatt gctgcgtttc catggcgcac tgcgcttata cttgtcgact gccccgctca
     2881 gggcgggcgc gtagtctagc agaaggccgg ggaacgattt gcgcttcgaa actgctgagg
     2941 tgagcctcct cggcgaccgt gacgagaacc aggatcgcgc tgccatccac aggaacaacg
     3001 gcagcgtgct gctggtggtg gccgacggca tgggcgggca cagtggcggc gcgctggcgg
     3061 ccgaagtggc ggtggattcg ctgtgccgcg ccttcgcgca ggccgcgccg gagcaggacg
     3121 gccgctcgtt cctccagggc gcagtcgagg cggcgcacga tgccgtggtc gccatcggcg
     3181 agaaggtcgg cgtcggcagc cggccgcgcg ccacctgtgc ggtctgcctg gtgcgggacg
     3241 atcacgccac ctgggcgcat gtcggcgatt cccgcgtcta cctcgcccgc gccggcgccg
     3301 tcgtgacgcg cacgcgcgac cacacgccca tcgaaagcct gctgcaggac ggcctcatca
     3361 gcgaggagga aatcgccggc cacccaatgc gccattacgt ggagtactgt ctcggcgggc
     3421 tggcggagcg accgctcatc acgatttcag agccggtgcc gctcgcagcc gacgacctgg
     3481 tggtggtgtg ctcggacggt ttctggtccg gcctggccga cgccgacatc ggcgccgggc
     3541 ccgagcaggg cgcgggactg caggactggc tgtcccgctt ggccggtcgg gcggtgcgcg
     3601 tcggcacgcc ctacagcgac aacaccacgg tggtcgccct cagggcgctg gcggcctgaa
     3661 cgacgattgg agaacccgat gagacccagc cagcgacgag ccgacgaact tcgcgccgtg
     3721 cgcttcgagc gcggcttcca gcgccacgcc gagggctcgg tgctggtcga gttcggcgcc
     3781 acacgggtgc tgtgcgccgc cagcgtcgag gatcgcgtgc cgccgttcct caagggcaag
     3841 ggcaagggct gggtgacggc cgagtacggc atgctgccgc gcgccacgca tacccgcaac
     3901 ccgcgcgagg cggcccgtgg ccgccaggga ggccgcacgc tcgagatcca gcgact
//
LOCUS       DAOMPG010000221         3923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9572_length_3923_cov_4.497156, whole genome shotgun sequence.
ACCESSION   DAOMPG010000221 DAOMPG010000000
VERSION     DAOMPG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_3923_cov_4.497156"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1146)
                     /gene="folE2"
                     /locus_tag="PLS34_10720"
     CDS             complement(310..1146)
                     /gene="folE2"
                     /locus_tag="PLS34_10720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRP87973.1"
                     /translation="MKDISEAGAISIDPIADVQGSADLRQIPINKVGIRDIVHPVRIK
                     ERAGGEQHTVAHFNMYVNLPHNFKGTHMSRFVEILNNHEREISVKSFRDMMSELTERL
                     DAESGHIEMSFPYFVNKTAPVSGVQSLMDYQASLIGERKNGESIIWAKVVVPVTSLCP
                     CSKKISDYGAHNQRSHVTIAVKVGGFVWLEELIELAEEEASCELFGILKRPDEKWVTE
                     RAYDNPKFVEDLVRDIAVRLNADDRIRAYTVESENFESIHNHSAYALIERDKDLDGPL
                     PR"
     gene            complement(1283..3172)
                     /gene="dxs"
                     /locus_tag="PLS34_10725"
     CDS             complement(1283..3172)
                     /gene="dxs"
                     /locus_tag="PLS34_10725"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRP87974.1"
                     /translation="MGLEDKYPLLSRIQAPADLRALPEDQLEPLTEELRAYLVETMSR
                     TGGHFAANLGTVELATVLHYVFDTPHDRLVWDVGHQAYPHKILTGRRDRLCSIRQLGG
                     LAPFPTRFESEYDTFGVGHSSTSVSAALGMAIAAKQKGERRRVVAVIGDGALTAGLAY
                     EALCHAGSLDVDLLVILNDNDMSISENVGGMSHYLARILSGKLYSTIREGSKKLIRRM
                     PTVWELARRSEEHVKGMVLPGTLFEELGMNYIGPVDGHDVDVLLKTLSNVRDLPGPQF
                     LHVVTRKGKGYAPAEADPITWHGPGPFDPEQGVIHKAPSKGKSYSAIFGDWLCDMAEA
                     DERIVAITPAMREGSGMVEYSRRFPGRYFDVGICEQHAVTLGAGLACEGLRPVVAIYS
                     SFLQRGYDQLIHDVALQNLPVVFALDRGGLVGNDGPTHHGAYDLSFMRCIPNMVVMAP
                     ADENECRQMLYTGVTLDGPSAIRYPRGRGPGAPIDSAMHALPLGKAELRRRGRGVALL
                     AFGAMVPVAEQVAADLDATVVNMRFVKPLDEEMVLAMAEGHGTLVTLEDNALMGGAGS
                     AVGECLAAHRVLVPLLNLGIPDHFIEHGSREECLAAAGLDAQSVLESVRAWQRRPELR
                     TVGQA"
     gene            complement(3302..>3923)
                     /locus_tag="PLS34_10730"
     CDS             complement(3302..>3923)
                     /locus_tag="PLS34_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRP87975.1"
                     /translation="DDDLRRGRPTCHRAFDEATAILVGDALQVLAFEILTEDQNMVPD
                     PARRLEIIRLLARASGTAGMAGGQAVDLEAAGQALSVAALEQMHRLKTGALIHASVLM
                     AAASADVLAPEQLRALEAYGSAIGLAFQVQDDILDVEGDTGVLGKRVQADAARAKPNY
                     PSVAGMAEAKRRLGELHDEALAALRPLGQAAEPLRWLSGWLTERRH"
BASE COUNT          571 a         1337 c         1362 g          653 t
ORIGIN      
        1 ccctatccgg ccaccatcgc cgtcgagctg agggatgctg acgagcagct cgtcgccagc
       61 gccgacatcc acatgtggct cagcccgcgg ccgggcagtc gccgccaaaa cgattgactg
      121 atcgacgccg cgccgagcag tcgactccgt gccggtcatt caacgctgca gcggctgggt
      181 gtgtcggtgg cggcctgcgc ggcgctcgcg cacaggacgt gcgcgaatga gcctacaggg
      241 atgtattcac ggcgtccgcg caggccgtcg ccggcgtacc cggccgcggc ccgcagtgac
      301 cgggaccact taccgaggca gcggcccgtc caagtccttg tcgcgctcga tcagcgcgta
      361 ggccgagtgg ttgtggatcg actcgaaatt ctccgactcc accgtgtagg cgcgaatccg
      421 gtcgtccgca ttcagccgca cggcgatgtc gcgcaccagg tcctcgacga acttggggtt
      481 gtcgtaggcg cgctcggtga cccacttctc gtccggtcgc ttgaggatgc cgaacagctc
      541 gcaggaggct tcctcctcgg ccagctcgat cagttcctcg agccagacga agccgccgac
      601 cttgaccgcg atcgtgacgt gcgagcgctg gttgtgggcg ccgtagtcgg agatcttctt
      661 ggagcagggg cacaggctgg tgaccggcac cacgaccttg gcccagatga tgctctcgcc
      721 gttcttccgc tcgccgatca ggctggcctg gtaatccatc aggctttgca cgccggagac
      781 cggcgccgtc ttgttgacga aatacgggaa gctcatctcg atgtggccgg actcggcatc
      841 gagccgctcg gtcagttcgc tcatcatgtc gcggaaagac ttcacggaga tctcccgttc
      901 gtggttattg aggatctcca cgaagcggga catgtgcgtg cccttgaagt tgtgcggcag
      961 gttcacgtac atgttgaagt gcgccacggt gtgctgctcg ccgccggcgc gctccttgat
     1021 gcgcacggga tgcacgatgt cgcggatgcc caccttgttg atgggaatct ggcgcaggtc
     1081 ggcgctgccc tggacgtctg cgatcggatc gatgctaatg gctccggcct cactgatgtc
     1141 tttcatgatc tgtttcccgg ttgggcttgt ggtctgacct gcccggcgtc cccgggcccg
     1201 tgcccccggc gcgaaagtca acttgcgggc ggctcgacgc tggtttcgcg gcggcgcccc
     1261 ggcgggattc actccccggt attcaggcct gtcccacggt acgaagttcc ggccggcgct
     1321 gccaggcgcg gaccgattcg agcacggact gggcgtccag cccggccgcg gcgaggcatt
     1381 cctcgcgcga gccgtgctcg atgaaatgat ccggaatgcc gagattcagc agcggcacca
     1441 gcacccggtg cgccgccagg cactccccca cggcgctgcc ggcgccgccc atcagggcgt
     1501 tgtcctcgag ggtgaccagg gtgccgtggc cctcggccat ggccagcacc atttcttcgt
     1561 cgagcggctt gacgaagcgc atgttcacca ccgtcgcatc gagatcggcg gctacctgct
     1621 cggcaaccgg caccatcgcg ccgaaggcca gcagggcgac gccgcggccg cgccggcgca
     1681 gttccgcctt gcccagcggc agcgcgtgca tggcgctgtc gatgggcgcg cccgggcccc
     1741 ggccgcgcgg gtaacggatg gcgctcgggc cgtccagcgt gacgccggtg tagagcatct
     1801 gccggcactc gttctcgtcc gccggcgcca tcaccaccat gttcggtatg cagcgcatga
     1861 acgacaggtc gtaggcgccg tgatgcgtcg gcccgtcgtt gcccaccagg ccgccgcgat
     1921 cgagcgcgaa caccaccggc agattctgca gggcgacgtc gtgaatcagc tggtcgtagc
     1981 cgcgctgcag gaagctcgag tagatggcga ccacggggcg caggccctcg caggccagcc
     2041 cggcgcccag cgtaacggcg tgctgctcgc agatgccgac gtcgaaatac cgcccgggaa
     2101 agcgccgtga atactccacc atgccggagc cctcgcgcat cgccggggtg atggcgacga
     2161 tgcgctcgtc cgcttccgcc atgtcgcaca gccagtcacc gaagatggcc gagtagctct
     2221 tgcccttgga cggcgccttg tggatgacgc cctgttcggg atcgaagggg cccgggccgt
     2281 gccaggtgat ggggtccgcc tcggccgggg cgtagccctt gcccttgcgg gtcacgacgt
     2341 gcaggaactg cggtcccggc aggtcgcgga cgttgctcag cgtcttgagc agcacgtcca
     2401 cgtcgtggcc gtcgaccggc ccgatgtagt tcatgccgag ctcttcgaac agcgtgccgg
     2461 gcagcaccat gcccttgacg tgctcctcgg agcggcgcgc cagttcccac accgtcggca
     2521 tgcggcggat gagtttcttg ctgccctcgc ggatggtcga atacagcttg ccggacagga
     2581 ttctcgccag gtaatgggac atgccgccga cgttctcgga gatggacatg tcgttgtcgt
     2641 tcaggatcac gagcaggtcg acgtccaggc tgccggcgtg gcagagggcc tcgtaggcga
     2701 ggcccgcggt cagcgcaccg tcgccgatga ccgccacgac gcggcggcgc tcgcccttct
     2761 gcttcgccgc gatcgccatg ccgagcgccg cgctcaccga ggtgctggag tggcccacgc
     2821 cgaaggtgtc gtactcgctc tcgaagcggg tggggaaagg cgcgaggccg ccgagctggc
     2881 ggatgctgca caaccggtcc cgccggcccg tgaggatctt gtgcgggtag gcctggtggc
     2941 cgacgtccca gaccagccgg tcgtgcggcg tgtcgaacac gtaatgcagc acggtggcga
     3001 gttccacggt gccgaggttg gcggcgaagt ggccgccggt ccgggacatg gtctcgacca
     3061 ggtaggcgcg cagctcctcg gtcaggggct cgagctggtc ttccggcagc gcccggagat
     3121 cggccggggc ctggattcgc gacaacagcg ggtatttgtc ctcgagtccc atgtcagagc
     3181 ttgccgtgcc ggccgaggtg acgaccaatc cggcgattat ccgccacagc ggcggcgctg
     3241 tccaaaggtc cgggacggga ttcgcggccc gcgacggacc gggccggccg gtcgccgggg
     3301 atcaatgccg ccgctcggtg agccagccgg acagccagcg cagcggctcg gccgcctggc
     3361 cgagcggccg cagcgccgcc aacgcctcgt cgtgcagctc gcccagccgg cgcttggcct
     3421 cggccatccc cgccacgctg gggtagttgg gcttggcgcg ggcggcatcg gcctgcacgc
     3481 gcttgccgag cacgcccgtg tcgccctcta cgtcgagaat gtcgtcctgt acctggaagg
     3541 ccagcccgat ggcggacccg taggcctcga gggcgcgcag ctgctccggc gccagcacat
     3601 cggcgctggc cgcggccatc aggacgctgg catggatgag tgcgccggtc ttgagccggt
     3661 gcatttgctc cagcgccgcg accgacagcg cctgcccggc tgcttcgagg tcgaccgcct
     3721 ggccgccggc catgccggcg gtgccggagg cgcgcgccag caggcggatg atctccaggc
     3781 gccgcgccgg gtccggaacc atgttctggt cctcggtgag aatctcgaag gcgagcacct
     3841 gcagggcgtc gcccaccagg atcgcagtcg cctcgtcgaa ggcgcgatgg caggtcggcc
     3901 ggccgcgccg caggtcgtcg tcg
//
LOCUS       DAOMPG010000222         3903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9624_length_3903_cov_3.106029, whole genome shotgun sequence.
ACCESSION   DAOMPG010000222 DAOMPG010000000
VERSION     DAOMPG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_3903_cov_3.106029"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="PLS34_10735"
     CDS             <1..1697
                     /locus_tag="PLS34_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP87976.1"
                     /translation="YSAVVVALSYLIDPETPKNDGAFRPVTVITREGTIVNPRPGKPV
                     TLGGRAKAFPVRQVSARGGEVLHWSGDSATLRWSYGPRLFERRLSDAFAFLSGAPEEL
                     PAPVTTGRDLSFRAPADRHDARIALVGGRVVTMRDAYQQEEIIDNGVVLIEGHRIRAV
                     GPAGELEVPAGFEVFDASGKTIIPGLVDAHAHGGMSNNQLQPRQNWMQYANLAFGVTT
                     IHDPSNDNWGFFSAAELQRSGRMLAPRMFSTGRILYGALFPGVTAKIDGYEDAEFHVR
                     RQQEQGAISVKSYNYLRRDQRQQVLKAGRELDVLVVPEGGMRLEQNLSQIVDGHTGIE
                     HSLPIQPIYEDILQLWSQTKVVYSPTFGVAYGGLMGEEYWYDRTEVWKNARLMAYTPE
                     SVVHPRSIRRPTAPDEHYNHVHVAEMARKLNDRGVPVVIGAHGQRAGLAAHWEIWMMV
                     QGGFSPFAALRGATIDGARYFGMEAEIGSIEPGKLADLVVIDGDPLADIRSSEKVAWT
                     MLNGRLYDAATMHQVAPAKIERRPFFFELEGGDAWQPATMQYFHEMGDALGWHCRH"
     gene            complement(1716..>2504)
                     /locus_tag="PLS34_10740"
     CDS             complement(1716..>2504)
                     /locus_tag="PLS34_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87977.1"
                     /translation="KRVAEEIHARQQAEAELAQVEAPRPKGLNGLGVGLGLLVGLLPA
                     LVLYLFYAQAAGERDAARAENTQLAAAVEQQSTLLESLRGELSASRDDEQFTADSRQL
                     AIVDALQWAANQGNDLDYDEIPFGDARIDSLRDLLERLAAIGFTGTVQLESHLGEFCL
                     SDDGAGGYRLAPPDTPLADCALVGHPLGGSTALRERLSVGFANFIATSPLLDDTGIAV
                     RLVAHGSRDSTRRYAFPLDLRTAGDWNRIATLNNRVEFALIPAG"
     assembly_gap    2505..2514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2515..3065)
                     /locus_tag="PLS34_10745"
     CDS             complement(<2515..3065)
                     /locus_tag="PLS34_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP87978.1"
                     /translation="MALKRALVVDDSKSARVALQRLLEQHGIAVSFAESGEEAIDFLK
                     HQAVDVIFMDHSMPGMDGFEAVSAIKADPRTAMIPVMMYTAKEGEVYVGQARALGAVG
                     VLPKQVQPGVLFEMLLKLGLVKERRAGTRRRRSDGERRAKAAAPATDQAELLLERQAL
                     GMSIQALITRILQDQHLELRSDVL"
     gene            3129..3482
                     /locus_tag="PLS34_10750"
     CDS             3129..3482
                     /locus_tag="PLS34_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRP87979.1"
                     /translation="MRRREFERLVDEVLDTLPDWALAGVANLRVVVEEWPSEEQDPDD
                     EGLLGLYEGIALPERGLDYVDVMPDTIWIFRQPHLELGLPPDELREEIRRTVLHELAH
                     YFGLDDDYLDEIGWG"
     gene            complement(3553..>3903)
                     /locus_tag="PLS34_10755"
     CDS             complement(3553..>3903)
                     /locus_tag="PLS34_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRP87980.1"
                     /translation="EIAEKDELNLRNPPEKTPELFERYLPFALALGVEQKWAEKFAAV
                     FARLAGQQGTSYRPAWYRGDFNAHNIGSFTTSVGSSLSSAISSASTAPGSSSGGGGGG
                     SSGGGGGGGGGGGW"
BASE COUNT          606 a         1360 c         1336 g          591 t
ORIGIN      
        1 cctactccgc cgtcgtcgtc gccctctcct acctgatcga tcccgagacg ccgaagaacg
       61 acggcgcgtt ccgccccgtg acggtgatca cgcgcgaggg cacgatcgtg aaccctcggc
      121 ccggcaagcc ggtcacgctc ggcggccgtg ccaaggcctt cccggtgcgc caggtctcgg
      181 cacgcggcgg cgaggtgctg cactggtcgg gcgacagcgc cacgctgcgc tggtcttacg
      241 gccccaggct gttcgagcgc aggctcagcg acgcgtttgc tttcctgtcc ggcgcgccgg
      301 aggaattgcc ggcgccggtg accactggcc gcgacctgtc gttccgcgca cccgcggatc
      361 gccacgacgc gcgcatcgcg ctggtcggcg gccgcgtcgt caccatgcgc gatgcctatc
      421 agcaggagga aatcatcgac aacggcgtgg tgctcatcga gggccatcgc attcgcgccg
      481 tgggcccggc cggcgagctc gaggtgccgg ccggcttcga ggtgttcgac gcctccggca
      541 agaccatcat ccccggcctg gtggacgcgc atgcccacgg cggcatgagc aacaaccagc
      601 tgcagccgcg ccagaactgg atgcagtatg ccaacctcgc cttcggcgtc accaccatcc
      661 acgacccctc gaacgacaac tggggctttt tctcggccgc cgagctgcag cgctccggcc
      721 ggatgctggc gccacgcatg ttctccaccg ggcggattct ctacggcgcg ctgttccccg
      781 gcgtgaccgc gaagatcgac ggctacgagg acgccgagtt ccacgtgcgc cgccagcagg
      841 agcagggggc gatctcggtg aagagctaca actacctgcg ccgcgaccag cgccagcagg
      901 tgctcaaggc cggccgcgag ctcgacgtgc tggtggttcc cgagggcggc atgcgcctcg
      961 agcagaacct cagccagatc gtcgacggcc acaccggcat cgagcacagc ctgccgatcc
     1021 agccgatcta cgaggacatc ctgcagctct ggagccagac caaagtggtc tactcgccga
     1081 ccttcggcgt ggcctacggc gggctgatgg gcgaggagta ctggtacgac cgcaccgagg
     1141 tgtggaagaa cgcgcgcctg atggcctaca cgccggagtc ggtggtgcat ccgcgctcga
     1201 tccgccgccc gaccgcaccg gacgaacact acaaccacgt gcatgtggcc gagatggcca
     1261 ggaagctcaa tgaccgcggc gtgccggtgg tgatcggcgc gcacggccag cgggccggcc
     1321 tggcggcgca ctgggagatc tggatgatgg tccagggcgg cttctcgccc ttcgccgcgc
     1381 tgcgcggcgc gaccatcgac ggcgcgcgtt atttcggcat ggaagcggag atcggctcga
     1441 tcgagccggg caagctggcc gacctggtgg tcatcgacgg cgacccgctc gcggacatcc
     1501 gcagcagcga gaaggtcgcc tggaccatgc tcaacggccg gctgtacgac gccgcgacca
     1561 tgcaccaggt ggcgccggcg aagatcgagc ggcggccgtt tttcttcgag ctggaaggcg
     1621 gcgacgcctg gcagccggcg accatgcagt acttccacga gatgggcgat gcgctggggt
     1681 ggcactgccg gcactgaggc cggccgctgc ggcgctcacc cggccgggat cagggcgaac
     1741 tcgacgcggt tgttcagggt cgcgatgcgg ttccaatcgc cggccgtgcg caggtcgagc
     1801 gggaaggcgt agcgccgcgt cgagtcgcgg ctgccgtgtg cgaccagccg cacggcgatg
     1861 cccgtgtcgt cgagcagcgg cgaggtggcg atgaagttgg cgaagccgac cgacaggcgc
     1921 tcgcgcagcg ccgtcgagcc acccagcggg tggccgacca gtgcgcagtc ggccaggggc
     1981 gtgtccggcg gcgcgaggcg gtagccgccc gcaccgtcgt cgctgaggca aaactcgccg
     2041 agatgcgact cgagctgcac cgtcccggtg aagccgatgg ccgcgagccg ctcgagcagg
     2101 tccctgagcg agtcgatgcg cgcatcgccg aaggggattt catcgtagtc gagatcgttg
     2161 ccctggttgg ccgcccactg cagcgcgtcg acgatcgcca gctggcgcga gtcggccgtg
     2221 aactgctcgt cgtcccggct ggcggatagt tcgccgcgca gcgactccag cagcgtggac
     2281 tgctgctcca cggcagcggc cagctgcgta ttctccgccc gcgcggcatc gcgctcgccc
     2341 gccgcttgcg catagaacaa gtaaagcacc agcgccggca gcaggccgac cagcagaccc
     2401 agcccgacgc cgaggccgtt caggcccttc gggcggggcg cttcgacctg cgccagctcg
     2461 gcctccgcct gctgcctggc gtggatttcc tcggcgacgc gcttnnnnnn nnnnagcacg
     2521 tcggatcgca gctcgagatg ctggtcctgc aggatgcggg tgatcagcgc ctggatcgac
     2581 atacccagcg cctggcgctc cagcagcagc tccgcctggt ccgtggcggg ggccgcggcc
     2641 ttggcgcggc gctcgccgtc gctgcggcgc cttcgcgtgc cggcgcgccg ttccttgacc
     2701 agcccgagct tgagcagcat ctcgaacagc acgcccggct gcacctgctt gggcagcacg
     2761 ccgacggcgc cgagggcgcg cgcctggccg acgtacacct ccccttcctt ggccgtgtac
     2821 atcatcaccg ggatcatcgc cgtgcgcgga tcggccttga tggcggacac cgcctcgaag
     2881 ccgtccatgc ccggcatgct gtgatccatg aaaatgacgt cgaccgcctg gtgcttcagg
     2941 aaatcgatcg cttcctcgcc cgattccgcg aaggagacgg cgatgccgtg ctgctcgagc
     3001 agccgctgca gggccacccg ggcggacttg gaatcatcga cgacgagggc acgcttgagc
     3061 gccatggcga cactttgttg tacttcgcgc ccttagtcca gacccccgcg gcctgatacg
     3121 ctgccggcat gcgccgacgc gaattcgaac ggctggtcga cgaggttctc gacaccctgc
     3181 ccgactgggc gctggcgggg gtggcgaacc tgcgcgtagt ggtcgaggaa tggccgagcg
     3241 aggagcagga cccggacgac gagggcctgc tggggctcta cgagggcatc gcgctgccgg
     3301 agcgcggcct cgactacgtg gacgttatgc cggacaccat ctggatcttc cgccagccgc
     3361 atctcgagct cggcttgccc ccggacgagc tgcgcgagga gatccgccgc acggtgttgc
     3421 acgagctggc acactatttc ggcctggacg acgactacct ggacgaaatc ggctggggct
     3481 gaaaaaaccg gggtcagaga caggtttccc gtcgggaaac ctgtctctga ccccggtttt
     3541 tgccgattcc tgttaccagc cgccgccgcc gccgccgcca ccaccaccac cggacgagcc
     3601 gccgccgcca ccgccggagc tggagcccgg cgcggtcgag gccgaggaga tggccgagct
     3661 gagcgagctg ccgacgctgg tcgtgaacga gccgatgttg tgtgcattga aatcgccccg
     3721 gtaccaggcc ggccggtagc tcgtcccctg ctggccggcg aggcgtgcaa acaccgcggc
     3781 gaacttctcc gcccatttct gctcgacgcc cagcgccagc gcgaacggca ggtagcgctc
     3841 gaacagctcc ggcgtcttct cgggcggatt gcgcaggttc agctcgtcct tctcggcgat
     3901 ctc
//
LOCUS       DAOMPG010000223         3901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9631_length_3901_cov_3.972439, whole genome shotgun sequence.
ACCESSION   DAOMPG010000223 DAOMPG010000000
VERSION     DAOMPG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9631_length_3901_cov_3.972439"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1989
                     /locus_tag="PLS34_10760"
     CDS             124..1989
                     /locus_tag="PLS34_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP87981.1"
                     /translation="MRPEWSQAVALLGLLVLALGLWFAWLGTRRLRTAQRAVPAAAEP
                     AAHDGQAADVTRWTYQFLELVLDRVQDGVIVCDEHGRVIEANEAAARLLQRDARTLAG
                     EDLHDLAEPSVFDDRDALLPEGSTRLKTPTEEATAVAYTSCQLVEPDGLRRWVLVIRN
                     LSEAHRTQKRIRYLARYDTLTRVPNRMEFQHRLQQAIARARRTTTRIALLYIDLDGFK
                     DINDRLGHPIGDRALEITSRRLVEAMPSGTLLGRLGGDEFALLIEGLPADRDSRPAVA
                     ATIRMLLDKLAQKFHVEGHEVVLTASFGVALFPDDADNVIDMIRNADAAMYHAKQNGG
                     NTYGFYAPEMNADAVDRLLLKTELRSAIRRNEFEVVYQPKIDLRDGRIAGCEALLRWH
                     HTRRGDVPPLVFIPLAEENTLIFEVGEWVLDRVCKDFSSWQQQVPWPGRVAVNLSLKQ
                     LRQRNFVAGVEKIFQRYELTPSCVELEITESTLIDHGEKTLRMLDRLYRLGLHLSIDD
                     FGTGYSSLSSLQHFPIGTLKIDQSFVREAGQERNSAAIVGAIVGLGRSLGMDVVAEGI
                     ETVDQLGLLQRLNCTFGQGHLFGTPVSADRYVEMLIAQQHGTPAHAALCRAIGSD"
     gene            complement(2020..>3901)
                     /locus_tag="PLS34_10765"
     CDS             complement(2020..>3901)
                     /locus_tag="PLS34_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP87982.1"
                     /translation="DIAAAQELLGMTGMLSRVDLIASPGLAAQLAATLPPGAELVASA
                     ARSQATFGMTRAFRLNLTALALLALLVGAFLIYNTMNFLVVRRTRTLGILRSVGVARG
                     GVVRAILSEALVVGVPGTLLGLLLGGLLGSGLTALVVQTIDDLYFRLRVDALALSGWP
                     FAKAAALGLGATLLAALGPALEAAGIPPRAVLSRASMERRTRRRLPWLLAAAALALLL
                     SVLLLAAGRNSLVVSFAGLFMVFLACACATPPATAGLMWLLDRALPARAPVPVRMAIR
                     GTSASLSRTGVSVAALAVSVATVIGVGLMVSSFRASVDDWLGQSLVADLQALPGVDEV
                     TFSLRRRLQAGGEEWRLWAVDAGRQGLGAEILAGDPAVARARFQAGEAVLVSEPWAMR
                     RGTRVGDLLALPTPAGEQVFPVAGVFRDYTSDRGVIALHRDRYRTLWGDECSEGIGVN
                     LAPGADTAAARTGIEGTLPPGSGISLSDNAGLRAASLAVFDRTFTITRVLQVLVALVA
                     FLGILSALQALQLERVRETAVLRAVGWLPRQLRALVVAQTGLLGLAAGLFALPLGIAL
                     AGLLVFVINRRAFGWSMSFGIDPGELLQGLALALAAAVLAGLYPAWRTSRRPVAEDLR
                     EE"
BASE COUNT          645 a         1418 c         1359 g          479 t
ORIGIN      
        1 acctgcaatc catgggagac acggacgtag tcaccttcga cgagcgccgc aagggctgag
       61 cgccgaccgc atgcgcccgc catccgcagt ccgcagcacg acccggccgg tcccgggatg
      121 ctgatgcgcc ccgaatggtc ccaggcggtc gcgctgctcg ggctgctggt cctggcgctc
      181 gggctgtggt tcgcttggct cggaactcgc cgactgcgca cagcgcagcg cgcggtcccg
      241 gcggcagcag agccggcggc gcacgatggg caggcggccg atgtgacgcg ctggacctac
      301 cagttcctgg agctggtgct cgaccgtgtc caggacggcg tgatcgtctg cgacgagcat
      361 ggccgggtca tcgaggccaa cgaggcggcc gcccggctgc tgcagcggga cgcccggacg
      421 ctcgccggcg aggacctgca tgaccttgcc gaaccgagcg tgttcgacga tcgcgacgcc
      481 ctgcttccgg aaggcagcac gcgcctcaag acccccaccg aggaggcgac cgcggtggcc
      541 tacaccagct gccaactggt ggagcccgat ggactgcgcc gctgggtgct ggtgatccgc
      601 aatctcagcg aagcgcatcg cacccagaag cgcatccgct acctggcacg ctacgacacg
      661 ctcacgcggg tcccgaaccg gatggagttc cagcaccggt tgcagcaggc gatcgccagg
      721 gcccgacgca ccaccacgcg catcgcactg ctctacatcg acctggacgg tttcaaggac
      781 atcaacgacc ggctcggcca tccgatcggc gaccgcgcgc tggagatcac ctcgcgccgg
      841 ctggtggagg ccatgccatc gggcaccctg ctcggccggc tcggcggcga cgagttcgcg
      901 ctgctcatcg agggcctgcc ggcggaccgt gattcgcggc cggcggtcgc cgcgaccatc
      961 cgcatgctgc tcgacaagct ggcgcagaaa ttccacgtcg aggggcacga ggtggtgctg
     1021 acggccagct tcggcgtggc gctgttcccg gacgacgccg acaacgtcat cgacatgatc
     1081 cgcaacgccg acgccgcgat gtatcacgcc aagcagaacg gcggtaacac ctacggcttt
     1141 tacgcgccgg aaatgaacgc cgacgccgtg gaccggctgt tgctcaagac cgagctgcgc
     1201 agcgccatcc ggcgcaacga gttcgaagtg gtctaccagc cgaagatcga cctgcgtgac
     1261 gggcgcatcg ccggctgcga ggcgctgctg cgctggcacc acacccggcg tggggacgtg
     1321 ccgccgctgg tgttcatccc gctggcggaa gagaacacgt tgatcttcga ggtcggcgag
     1381 tgggtgctcg atcgcgtgtg caaggacttt tcgagctggc agcagcaggt gccctggccc
     1441 ggccgcgtgg cggtcaacct gtcgctgaag cagctgcggc agcgaaactt cgtggcgggc
     1501 gtggaaaaga tcttccagcg ctacgaactg acgccctcct gcgtggagct ggagatcacg
     1561 gagtcgaccc tcatcgacca tggcgaaaag acgctgcgca tgctcgaccg gctctaccgg
     1621 ctcggcctgc acctgtccat cgatgacttc ggcaccggct attcctcgct gtccagcctg
     1681 cagcacttcc cgatcggcac gctgaagatc gaccagagtt tcgtgcggga ggccggccag
     1741 gagcgcaaca gcgccgccat cgtgggcgcc atcgtcggcc tgggccgcag cctgggcatg
     1801 gacgtggtcg cggaaggcat cgagacggtg gaccagctcg gcctgctgca gcgcctcaac
     1861 tgcactttcg gccagggtca cctgttcggc acgccggtat cggcagatcg ctacgtggag
     1921 atgttgatcg cccagcagca cggcacgccg gcgcacgcgg cgctgtgccg cgccatcggc
     1981 agcgactgac gactcggcgc ctgccggggc ggcgttgcgt tattcctcgc gcagatcctc
     2041 ggccacgggc cgacggctgg tgcgccacgc cgggtagagc ccggccagca cggcggcggc
     2101 gagcgccagc gccaggccct gcagcagctc gcccgggtcg atcccgaagc tcatgctcca
     2161 gccgaaggcg cgccggttga tgacaaacac gagcaggccg gccagcgcga tgcccagcgg
     2221 cagcgcgaac aggcccgcgg cgagccctag caacccggtc tgggccacta ccagcgcgcg
     2281 cagctggcgc ggcagccagc cgaccgcccg cagcaccgcc gtctcccgca cgcgttccag
     2341 ctgcagcgcc tgcagcgcgg agaggatgcc gagaaacgcc accagcgcca ccagcacctg
     2401 cagcacgcgg gtgatggtga aggtccggtc gaacaccgcc agcgaggcgg cacgcagccc
     2461 ggcattgtcg gacagcgaga tgccgctgcc gggcggcagc gtgccctcga tccctgtgcg
     2521 cgccgccgcg gtgtccgcgc cgggtgccag gttgacgccg atgccctcgc tgcactcgtc
     2581 gccccacagc gtccggtaac gatcccggtg cagcgcgatg acgccgcgat ccgaggtgta
     2641 gtcgcgaaac accccggcga ccggaaagac ctgttcgccg gcgggcgtcg gcagggccag
     2701 caggtcgccc acgcgggtgc cgcgacgcat cgcccagggc tcggacacca gcaccgcctc
     2761 gcctgcctgg aaccgggccc gtgccacagc cgggtcgccc gccaggatct cggcgcccag
     2821 gccctggcgg cccgcatcca cggcccacag gcgccactcc tcaccgccgg cctgcaggcg
     2881 cctgcgcagc gaaaaggtca cctcgtccac gcccggcagc gcctgcaggt ccgcgaccag
     2941 cgactggccc agccagtcgt ccacgctcgc ccggaagctg ctgaccatca gcccgacccc
     3001 gatgacggtc gccaccgaca cggccagcgc cgcgaccgag acgccggtcc ggctgaggga
     3061 ggcgctggtg ccgcggatcg ccatccgcac cggcaccggc gcgcgtgccg ggagcgcacg
     3121 gtccagcagc cacatcagtc ccgcggtcgc cggcggggtg gcgcaggcgc aggcgagaaa
     3181 gaccatgaaa agccctgcga aggacaccac cagcgagttg cgcccggcgg cgagcagcag
     3241 cacggacaac agcaaggcca gcgcagccgc cgccagcagc cagggcagcc ggcggcgcgt
     3301 gcgccgctcc atcgaggcgc gcgacagcac cgcgcggggc gggatgccgg ccgcctccag
     3361 cgccgggccc agcgccgcca gcagcgtcgc gccgagtccg agcgcggcgg ccttggcgaa
     3421 cggccagccg ctcaaggcca gggcgtcgac ccgcagccgg aagtagaggt cgtcgatggt
     3481 ctgcacgacc agcgcggtga ggccggagcc gagcaggccg ccgagcagca ggccgagcaa
     3541 ggtccccggc acaccgacca ccagcgcctc ggacaggatg gcgcgcacca ctccgccgcg
     3601 cgccactccc acgctgcgca ggatgccgag ggtgcgggtg cggcgcacca ccaggaagtt
     3661 catggtgttg tagatcagga aggcgcccac cagcaacgcc agcagcgcca gtgcagtgag
     3721 gttcagccgg aaggcccgcg tcatgccgaa ggtggcctgc gagcgggcgg ccgacgccac
     3781 cagctcggcg cccggcggca aggtcgccgc cagctgcgcg gccagcccgg gtgaggcgat
     3841 gagatcgacg cggctgagca tgccggtcat gccgagcagt tcctgcgcgg cggcgatgtc
     3901 t
//
LOCUS       DAOMPG010000224         3888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9672_length_3888_cov_4.932168, whole genome shotgun sequence.
ACCESSION   DAOMPG010000224 DAOMPG010000000
VERSION     DAOMPG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9672_length_3888_cov_4.932168"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="PLS34_10770"
     CDS             <1..2250
                     /locus_tag="PLS34_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP87983.1"
                     /translation="RTVAQSQSPIDIITPELLQATGTTELATALARALPSLNFPRPAI
                     ADGTSGVRPAQLRGLAPDQVLILVNGKRRHVSALVNVNGTIGRGSSAVDLNAIPVSAI
                     ERVEVLRDGASAQYGSDAIAGVVNLVLKGAGSGGSIAARYGEYSEGDGQQWQLAADGG
                     LSFGEGAGSVHLTAETGHQDITNRSGAYTGSAPHTGNFPGVGEATFKIGDPEVEATSV
                     AANASLQVADDTRFYATLMGSDRDIDSFAFYRSKNHSGQGALLAQVYPNGYVPEINQK
                     SKDRSFVLGANGGSEGGWSWDVSYDYGRNTLDFRTQNTINYALGANSPRSFYDGSLAY
                     TQQVINADFSRPVNIGLDYPATLSLGTEYRKETWEQGAGEPDSYFGSGAQGFGGFTPR
                     NAGRHERHNIAAYAGLEADFSDAFSGGVAGRYEDYSDFGSEFSGKLSGRYAFTDAAAL
                     RATVASGFRAPSLAQQNYQAVTTNITNGVAYEAGTFPAAGPVARALGAEPLQAETSLS
                     YSLGLVLQPTDALYFTIDAYEISIDDRIVLSSNLLLGSNQAAQDLLASLGISGVTSAR
                     YFNNAIDTRTRGVDVVGTWSIPLASSNLDLTASYNRNKTDVTRVAPNPSQLDALGANL
                     ERMGRDERGRIEEGFPRDKFALGGNWTLERWTFNLGSSRYGEFTTRHASNPAQDQRYG
                     AKWVIDLSASYRPTENWSFTLGADNVTDAYPDKVIAANSTFGLIPYSLQSPFGFNGRY
                     YYARAGYSW"
     gene            complement(2362..>3888)
                     /locus_tag="PLS34_10775"
     CDS             complement(2362..>3888)
                     /locus_tag="PLS34_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRP87984.1"
                     /translation="SGNFQQNNFGRGGAGNDAVRADALDGSGTNNANFSTPGDGSPGR
                     MQMFRWTSPGGVEVTAPYAATYQSPVPDDWGGSFTSLSGEVVPVQAPTTGLLGCEGPY
                     TNAAAVSGKIALVKRGSCEFGQKALVAQQNGAAGVIIFNNDGTNTGAGMAAGASGASV
                     TIPVVGMLGNQDGDALLAAAAGAPVMVTMSPKLFPDRDSDFDAGIIVHEYGHGVSNRL
                     TGGTQSGCLSGDEQAGEGWSDFYGLMFTMTDAVCDLPRGVGTYSSFEPGTGRGIRRYP
                     YSPDMAVNPFTFADVADSAQSVPHGVGSVWATMLWDMSCKLVDRYGYEPDIHGRAGGN
                     GLALQLVTDGLKLQGCRPTFVMGRNGILAADAALDAADPAYLGNKCIIWHAFARRGLG
                     NAASSGSVNSRTDQTPDFTVPAECQSFQVQVNVTGPGTVGPAASPVTAAYEDVLGFTL
                     TPAAGGTIASATGCEGTLAGNTFTTLPIVRNCTIDVVFDGVPLPDAIFADGFEEPVTP
                     "
BASE COUNT          625 a         1393 c         1302 g          568 t
ORIGIN      
        1 cgcaccgtgg cgcaatcgca gtcgcccatc gacatcatca cgcccgaact gctgcaggcg
       61 accggcacca ccgagctggc cacggcgctg gcgcgggcgc tgccctcgct caacttcccg
      121 cgcccggcca tcgccgacgg caccagcggc gtgcgtccgg cgcagctgcg cggcctggca
      181 cccgatcagg tgctgatcct ggtcaacggc aagcgccgcc acgtctcggc gctggtgaac
      241 gtcaacggca ccatcgggcg cggctcctcg gcggtggatc tcaacgcgat cccggtcagc
      301 gccatcgagc gggtcgaggt gctgcgcgac ggcgcctcgg cgcagtacgg ctccgacgcc
      361 atcgccggcg tggtcaacct ggtgctcaag ggcgcgggat cgggcggcag catcgccgcg
      421 cgctatggcg agtattccga gggcgacggc cagcagtggc agcttgcggc cgatggcggg
      481 ctgagcttcg gcgaaggggc cggcagcgtg cacctgaccg ccgagaccgg ccaccaggac
      541 atcaccaacc gctccggtgc ctacaccggc tcggcaccgc acaccggcaa cttcccgggc
      601 gtgggcgagg ccaccttcaa gatcggcgat ccggaagtgg aagcgacttc cgtggccgcc
      661 aacgccagcc tgcaggtggc cgacgatacc cgcttctacg ccaccctgat gggcagcgac
      721 cgcgacatcg actcgttcgc cttctaccgc tcgaagaacc acagcggcca gggcgcgctg
      781 ctggcgcagg tgtatccgaa cggctatgtg cccgagatca accagaagtc gaaagaccgc
      841 agcttcgtgc tcggcgccaa cggcggctcg gagggcggct ggagctggga cgtgagctac
      901 gactacggcc gcaacaccct cgatttccgc acccagaaca ccatcaacta cgcgctcggt
      961 gccaacagcc cgcgcagctt ctacgacggc tcgcttgcct acacccagca ggtgatcaac
     1021 gcggacttct cccgccccgt aaacatcggg ctggactatc cggccacgct ctcgctcggc
     1081 accgagtacc gcaaggagac ctgggagcag ggcgccggcg agccggattc ctatttcggc
     1141 tcgggcgcac agggctttgg cggcttcacc ccgcgcaacg ccggccgcca cgagcgccac
     1201 aacatcgccg cctatgccgg gctggaagcg gacttcagcg acgcgttttc cggcggcgtg
     1261 gccgggcgct acgaggacta ctcggacttc ggcagcgagt tctcgggcaa gctctcgggc
     1321 cgctacgcct tcaccgacgc ggcggcgctg cgcgccaccg tggccagcgg cttccgcgcg
     1381 ccctcgctgg cgcagcagaa ctaccaggcg gtcaccacca acatcaccaa cggcgtggcc
     1441 tacgaggccg gcaccttccc ggcggccggg ccggtggcgc gcgcgctggg tgccgagccg
     1501 ctgcaggcgg aaacctcgct ctcctacagc ctgggcctgg tgctgcagcc gaccgacgcg
     1561 ctgtacttca ccatcgacgc ctacgagatc agcatcgacg accgcatcgt gctgtcgtcc
     1621 aacctcctgc tcggaagcaa tcaggccgcg caggatcttc tcgccagcct gggcatctcc
     1681 ggcgtcacca gcgcgcgcta cttcaacaac gccatcgaca cccgcacccg cggtgtggac
     1741 gtggtgggca cctggtcgat cccgctggcg tccagcaacc tcgacctgac cgccagctac
     1801 aaccgcaaca agaccgacgt gacccgcgtc gcgcccaatc cgtcccagct cgatgccctc
     1861 ggtgccaacc tcgaacgcat ggggcgcgac gagcgcggcc gcatcgagga aggcttcccg
     1921 cgcgacaagt tcgccctggg cggcaactgg acgctggagc ggtggacctt caacctcggt
     1981 tcgagccgct acggcgaatt caccacgcgc cacgccagca atccggcgca ggaccagcgc
     2041 tacggtgcca agtgggtgat cgacctctcc gccagctacc ggccgaccga gaactggtcg
     2101 ttcacgctgg gcgcggacaa cgtcaccgac gcctacccgg acaaggtgat cgcggccaac
     2161 tccacgttcg gcctgatccc ctacagcctg cagtcgccgt tcggcttcaa cggccgctac
     2221 tactacgcgc gcgccggcta cagctggtaa cgtcggatcg gcatggggga aacgagaacg
     2281 ccccggccat gccggggcgt tctgcattgc gcctgccgaa ctcgccgccg ggcgcaggcg
     2341 ggcctgcgcc cggcgacgac gtcagggcgt gacaggctct tcgaaaccgt ccgcgaaaat
     2401 cgcgtcgggc agcggtacgc cgtcgaacac cacatcgatg gtgcagttgc gcacgatcgg
     2461 aagggtggtg aacgtgttgc cggcgagcgt gccttcgcag cccgtggccg aggcgatcgt
     2521 gccgcccgcg gccggcgtga gggtgaagcc caggacgtcc tcgtaggccg cagtgacggg
     2581 cgaagccgcc ggacccaccg tgcccggacc ggtcacgttc acctgcacct ggaagctctg
     2641 gcattcggcc ggaacggtga agtccggggt ctggtcggtg cggctgttga cgctgccgct
     2701 gctggcggca ttgcccaggc cgcggcgcgc gaaggcgtgc cagatgatgc acttgttgcc
     2761 caggtaggcc ggatcggccg catccagcgc ggcgtcggcg gcgaggatgc cgttgcgccc
     2821 catcacgaag gtcggccggc aaccctgcag cttgaggcca tcggtcacca gctgcagcgc
     2881 cagtccattg ccgcccgcgc ggccgtggat atccggctcg tagccgtagc ggtccaccag
     2941 cttgcagctc atatcccaca gcatcgtggc ccagaccgaa cccacgccgt gcggcaccga
     3001 ctgtgcgcta tcggccacgt cggcaaaggt gaacggattg accgccatgt ccggcgaata
     3061 cggatagcgc cggatgccgc ggcccgtgcc gggctcgaag ctcgaatagg tgcccacgcc
     3121 gcgcggcagg tcgcagaccg cgtcggtcat ggtgaacatc aggccgtaga agtcgctcca
     3181 gccctcgccg gcctgctcgt cgccggacag gcagccggac tgcgtgccac ccgtcaggcg
     3241 gttcgacacg ccgtggccgt actcgtgcac gatgatcccg gcatcgaagt cgctgtcgcg
     3301 atccgggaag agcttgggac tcatcgtgac catgaccggc gcgcccgcgg cggcggccag
     3361 cagggcatca ccgtcctggt tgcccagcat accgaccacc gggatggtga cgctggcacc
     3421 gctggcaccg gcggccatgc ctgcgccggt gttggtgccg tcgttgttga agatgatgac
     3481 gccggcggca ccgttctgct gcgcgacgag cgctttttgg ccgaactcgc aggagccgcg
     3541 cttgaccagg gcgatcttgc cgctgaccgc ggcggcattg gtgtaggggc cttcgcagcc
     3601 caggaggccg gtggtcggtg cctgcaccgg caccacttcg ccgctgagcg aggtgaagct
     3661 gccgccccag tcgtccggca ccggcgactg gtaggtggcg gcgtagggcg cggtgacctc
     3721 gacgccaccg ggcgatgtcc agcgaaacat ctgcatccgg ccggggctgc cgtcgccggg
     3781 cgtggagaag ttggcgttgt tggtgcccga gccgtcgagc gcgtcggcgc gcaccgcgtc
     3841 gttgcccgct ccgccgcggc cgaagttgtt ctgctggaag ttgccgga
//
LOCUS       DAOMPG010000225         3875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9717_length_3875_cov_3.835340, whole genome shotgun sequence.
ACCESSION   DAOMPG010000225 DAOMPG010000000
VERSION     DAOMPG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_3875_cov_3.835340"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLS34_10780"
     CDS             complement(<1..936)
                     /locus_tag="PLS34_10780"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRP87985.1"
                     /translation="MAVSPSVFGDRHSLSLGRQIMTRLRLDAPLLVGLLLICGFGLVV
                     LWSAGGQDTGLLLRQVVRLGVAFGVLLAVAQVPPKLLRLWSPWLFLGGLLLLVAVLVA
                     GDVGKGAQRWLDLGIVRFQPSEIMKLAVPMMAAWYLHDRPLPPRLVELVVVLALVVTP
                     ALLIARQPDLGTALLVGSAGLLVVLLGGLRLRLIVLLGAATAALAPVLWHFMHDYQRR
                     RVLTLFNPESDPLGAGYHIIQSKIAIGSGGLFGKGWGNGSQAQLEFLPERSTDFIFAV
                     LGEEFGLLGLVFLIAAYLFLVGRGMYIALQAQDTFT"
     gene            complement(938..2794)
                     /gene="mrdA"
                     /locus_tag="PLS34_10785"
     CDS             complement(938..2794)
                     /gene="mrdA"
                     /locus_tag="PLS34_10785"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRP87986.1"
                     /translation="MSPDRLKDHWREQRMFLSRLIAAALIVVLLAGLVVYRLVDLQVV
                     HFEHFSRLSQGNRVRIEPVPPVRGLLFDRRGRLLAENRPVYQLELVPEQVRDIEGTLA
                     ELIELELVREEDLPRIRTQIRSQRRFEPAILRPRLSEEEAARFAVKRPRFTGVDIQAR
                     LARQYPLGPVGSHAIGYVGAISESDLQRLDTANYAGTAQTGRIGAERAWEHLLHGTTG
                     YRQILVNAQGRTLQELERTDAVPGRNVYLTIDLDLQAAAEQAMQGRRGSVVALDPRNG
                     EVLALVSLPGYDPNLFSTGISANQYRALQEDIDQPLFNRALRGQYPPGSTIKPILALA
                     GLHYGVIDGHDTVFCPGWFSLPNHTHRYRDWKRTGHGTIDLENSVAQSCDVYYYRLAV
                     TLGIERMHEFLVQFGLGHVTGIDIPGEQTGLVPSRDWKRTAFRRPQDQVWFPGETVIT
                     GIGQGYMLATPLQLAQAAAIMGARGHGFRPTLLAAVEDPITGERQPAERHSLPPLKIA
                     PGPFEEAVDSMVAVVHGARGTARASGQGSRWTIAGKTGTAQVIGIAQGEEYDEEQVDE
                     RHRPHALFIAFAPAENPRIAVGVLVENGASGSAAAAPVARAVIDHWLQREED"
     gene            complement(2791..3282)
                     /gene="mreD"
                     /locus_tag="PLS34_10790"
     CDS             complement(2791..3282)
                     /gene="mreD"
                     /locus_tag="PLS34_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRP87987.1"
                     /translation="MSATAGPRSWPVYLSLLVALSLEVTPLPAELGAWRPPWLALTAI
                     YWALAYPRRYGLALAWFAGLVLDVLKGGVLGQHALAMTVATWLTLRFHLRLRVFPIWQ
                     QTVAVTAIVVTHQFVVFWVDGVTGGAELSWQRATPTVAAVIAWPVLVAVLDAVRLRLR
                     MLA"
     gene            complement(3279..>3875)
                     /gene="mreC"
                     /locus_tag="PLS34_10795"
     CDS             complement(3279..>3875)
                     /gene="mreC"
                     /locus_tag="PLS34_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRP87988.1"
                     /translation="KQKGMWMGGNPPLGYDVVDRKLVINEPEAERVRDIFRRYAGLKS
                     VRALKEELDADGIVGQVVRVNPLGAEAILISDPSHATPVELNRNGLRTVALGMGDVTR
                     MDLPFLPNSADIRIGDLLTSSGLGDAFPAGYPVARVTRVERRPGESFARVEAEPTAAL
                     NRTRQVLLVWEGGEAEASPFVAPPPESGGAGPAEEGVQ"
BASE COUNT          630 a         1356 c         1347 g          542 t
ORIGIN      
        1 ggtgaaggtg tcctgcgcct gcagggcgat gtacatcccg cgtcccacga ggaacaggta
       61 ggctgctatc aggaacacca gcccgagcag cccgaactct tcaccgagca ccgcgaaaat
      121 gaagtcggtc gagcgctcgg gcaggaattc cagctgcgcc tgcgagccgt tgccccagcc
      181 cttgccgaac aggccgccgg agccgatggc gatcttcgac tggatgatgt ggtacccggc
      241 gcccagcggg tcggattccg ggttgaacag ggtcagcacg cggcgccgct ggtagtcgtg
      301 catgaaatgc cagagcaccg gcgccagcgc ggcggtggcc gcgccgagca gcacgatcaa
      361 ccggagccgg agcccgccga gcagcaccac cagcaggccg gcgctgccga ccagcagcgc
      421 cgtgccgagg tccggctgtc gcgcgatgag cagcgccggc gtcaccacca gcgccagtac
      481 taccaccagt tccaccaggc gcggcggcag cggccggtcg tgcaggtacc aagccgccat
      541 catcggcacc gccagcttca tgatctccga gggctggaac cggacgatgc cgagatcgag
      601 ccagcgctgt gcccccttgc cgacgtcacc ggcgaccagc acggccacga gcaacagcag
      661 gccgccgagg aacagccacg gcgaccatag acgaagcagt ttcggcggca cctgtgccac
      721 tgcgagcaat acgccaaagg cgacgccgag gcggaccacc tggcgcaaca acaggccggt
      781 gtcctggccg ccggcgctcc acaggaccac gagcccaaag ccgcagatca gcagcagccc
      841 gaccagcaac ggcgcgtcca gccgcagccg ggtcatgatc tggcgcccga ggctcaggga
      901 atggcggtcg ccgaacaccg agggcgaaac ggccatctca gtcctcctcc cgctgcagcc
      961 agtggtcgat gacggcgcgc gccaccggcg ctgccgcggc gctgccgctg gcgccgttct
     1021 cgaccagcac gcctaccgcg atgcgcgggt tctcggccgg cgcgaaggcg atgaacagtg
     1081 cgtgcgggcg atgccgctcg tcgacctgct cctcgtcgta ttcctcgccc tgcgcgatgc
     1141 cgatgacctg cgccgtgccg gtcttgccgg cgatggtcca gcgcgagccc tggcccgagg
     1201 cccgggccgt gccgcgtgcg ccgtgcacca ccgccaccat ggaatcgacg gcttcctcga
     1261 aggggccggg cgcgatcttg agcggcggca gggaatgtcg ctcggcaggc tggcgctcac
     1321 ccgtgatcgg atcttccacg gcggccagca gggtgggccg gaatccatgg ccgcgggcac
     1381 ccatgatggc cgccgcctgg gccagctgca gcggggtggc gagcatgtac ccctggccga
     1441 tgccggtgat gaccgtttcg ccgggaaacc agacctggtc ctgcggccgg cggaaggccg
     1501 tgcgcttcca gtcgcgtgat gggaccaggc cggtctgctc gcccggaatg tcgataccgg
     1561 tgacatggcc gaggccgaac tggacgagaa actcgtgcat gcgctcgatg cccagcgtca
     1621 ccgccaggcg gtagtaatac acgtcgcagg actgcgccac cgaattctcc aggtcgatgg
     1681 tgccgtgccc ggtccgcttc cagtcgcgat agcggtgcgt gtgattcggc aggctgaacc
     1741 agccgggaca gaatacggtg tcgtggccgt cgattacgcc gtaatgcagc cccgccagcg
     1801 ccaggatcgg cttgatcgtg gagccgggcg ggtactggcc ccgcagcgca cgattgaaca
     1861 agggctggtc gatgtcctcc tgcagggcgc ggtactggtt ggcggaaatg ccggtgctga
     1921 acaagttcgg gtcgtagccc ggcaggctga ccagcgccag cacctcgccg ttgcgcgggt
     1981 ccagcgccac caccgagccg cgccggccct gcattgcttg ctccgcggcc gcctgcaggt
     2041 cgaggtcgat ggtcaggtag acgttgcgac ccggcaccgc gtcggtccgc tccagttcct
     2101 gcagtgtgcg cccctgggca ttgaccagga tctggcggta gccggtcgta ccgtgcagca
     2161 ggtgctccca ggcgcgctcg gcgccgatcc ggccggtctg cgcggtgccg gcgtagttgg
     2221 cggtgtccag gcgctgcagg tcgctctcgc tgatggcgcc gacatagccg atggcatgcg
     2281 agccgacggg cccgagcggg tactggcgcg caaggcgcgc ctggatgtcc acgcccgtga
     2341 agcgtggccg cttgaccgcg aagcgcgccg cttcttcctc gctgagccgc gggcgcagga
     2401 tggccggctc gaagcgccgc tggctcctga tctgggtgcg gatgcgcggc aggtcctcct
     2461 cccgcaccag ttccagctcg atcagctcgg cgagcgtgcc ctcgatgtcc cgcacctgct
     2521 cgggcaccag ttcgagctgg tagacgggcc ggttctccgc cagcagccgc ccgcggcggt
     2581 cgaacaacag gccgcgcacc gggggcaccg gctcgatgcg cacccggttg ccctgcgaaa
     2641 gccggctgaa atgctcgaag tgcacgacct gcaggtccac cagccggtaa accaccagcc
     2701 ccgccagcag caccacgatg agggcggcgg cgatcagccg gctgaggaac atgcgctgct
     2761 cgcgccagtg gtccttgagg cgatccgggc tcatgccagc atacgcagtc gcagcctgac
     2821 cgcatcgaga acggcgacga ggaccggcca ggcgatgact gccgcgacgg tcggcgttgc
     2881 gcgttgccag ctgagctcgg ccccgccggt gacgccatcc acccagaaca ccacgaactg
     2941 gtgcgtcacg acgatcgcag tgaccgccac ggtctgctgc cagatgggga acactcgcag
     3001 gcgaaggtga aacctcagcg tgagccaggt ggcgacggtc atggcgaggg catgctggcc
     3061 gagcaccccg cccttgagta cgtcgagcac caggccggcg aaccaggcca gtgcgagccc
     3121 gtagcgacgc ggataggcca gcgcccagta gatcgcggtc agcgccagcc acggcggccg
     3181 ccacgcgccg agttccgccg gcaacggcgt cacctcgagc gaaagcgcca ccagcagcga
     3241 caggtatacc ggccagctgc gcggtcctgc cgtcgcgctc actggacccc ttcctccgcc
     3301 ggtccggcgc cgccggactc cggcggcggg gcgacgaacg gggaggcctc ggcttcgccg
     3361 ccctcccaga ccagcagcac ctggcgggtg cggttgagcg cggccgtcgg ctccgcctcg
     3421 acgcgggcga aagactcgcc cgggcgacgt tcgacccggg tgacgcgcgc cacgggataa
     3481 cccgcgggga aggcgtcgcc gagcccggag gacgtcaaca ggtcgccgat gcggatatcc
     3541 gcgctgttcg gcaggaacgg caggtccatg cgcgtgacgt cgcccatgcc cagcgccacg
     3601 gtgcgcaggc cgttgcggtt gagttcgacg ggcgtggcgt ggctggggtc gctgatcagg
     3661 atggcctctg cgcccagcgg attgacgcgt accacctggc cgacgatgcc gtccgcgtcg
     3721 agctcttcct tgagggcgcg aacggacttg agcccggcgt agcggcgaaa gatgtcgcga
     3781 acacgctcgg cctctggctc attgatgacg agtttcctgt cgacgacgtc gtagccgagc
     3841 ggtggattgc cgcccatcca catgcccttc tgctt
//
LOCUS       DAOMPG010000226         3869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9731_length_3869_cov_3.625328, whole genome shotgun sequence.
ACCESSION   DAOMPG010000226 DAOMPG010000000
VERSION     DAOMPG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9731_length_3869_cov_3.625328"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLS34_10800"
     CDS             <1..308
                     /locus_tag="PLS34_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP87989.1"
                     /translation="SVRLARVRGGGLQPFPLPGTLEVADADALDRALLVHGARHTHLA
                     CLSPWSLAPPEHGRDCLGLMPALAVADSIAWRRPAGEFTAEWYCETLRFAWAVALRN"
     gene            364..2229
                     /locus_tag="PLS34_10805"
     CDS             364..2229
                     /locus_tag="PLS34_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRP87990.1"
                     /translation="MDTIATLALPDTHGLAVIGLALFALVALTRPSVPQESTSLMVLM
                     LLVLGFELFPYQGTAGGMGSGAFLSMFGNSALITVIALLVVGRALEVTGALQPLIRLM
                     LKVWTGPRSLALIVTLLAAAFASAFLSNTLVVLALLPVLVGASTRRRFPASQVLMPMG
                     FAALLGGMMTTIGTSTNLLVVNEAERLGLPDFGLFHFILPAAVGVAVGLLFLWLVAPR
                     LLPERDAPIRQAAPRIFKAMFFVGEDSWAVGRTLAELRERTGRRMNVSRVQRGDGLQV
                     AKLPAMRLQSGDRLMVADTRERLKEFERLLGVRMHSEFAGEEDDGTEEEAAGQQLAEL
                     VVTPDSTLYGRTLSTTQFRQRYRLVPLAIHRATAAGTSEVTGDFEDAVLQAGDVILVQ
                     GTPERLRELRASGSMLVLDGRVDLPKTTRAPLALGLTAAIVLAAGAGWTPISVSATAG
                     AALLIATGCIDWREVAGALSAPLLLIIVMSLALVEASVGTGADLFVAGLFLALFDGLP
                     PAAALALVLLLVSVAGSLAPNTAVALLAVPVAVGIARGLGAPLEPFVLAVLFGANLSF
                     ATALGSRPNLVVSTAGGYLARDFMRVGVPLTVLVWLAISLALVVRYAVPWGWPAG"
     gene            2474..3835
                     /locus_tag="PLS34_10810"
     CDS             2474..3835
                     /locus_tag="PLS34_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRP87991.1"
                     /translation="MHSPFDIAADLDTPVSAYMKLAPFRPRFLLESVEGAERLGRYSF
                     IGFGDALTVRLDRAGLHFGGRTRPVDGDLLPAMRAALAAAPTLAPEAGLPFDGGLVGI
                     AAYDIVRHFERVPGGSGDFPEGLWVAPRSLLVFDHLTRRAALLHAGDEAERLALRREV
                     VQALAGGVPFRRAGRHAPPAASMSEAEFLAAVGKAKGNITAGDVFQLVLSVRFSGRSD
                     IAPFEAYRALRLLNPSPYMYFLELDGLSVAGSSPEALVKLDGRHASLRPIAGTRPRGE
                     TREQDLAREVELLDDPKENAEHVMLVDLARNDLGRVAVPGSIRVEPSRAIERYSHVMH
                     IVSGVQGELEAGRDAFDLFAATFPAGTLVGAPKVRAMELIADYEPVGRGLYAGTVGYF
                     SRRGGMDQAIAIRTLAFRGDEYSFQAGAGIVADSVPLNEYREVLAKSAILRRALEMAE
                     EGL"
BASE COUNT          450 a         1360 c         1439 g          620 t
ORIGIN      
        1 catcggtacg cctggcgaga gtgcgcggcg gcggcttgca gcccttcccg ctgccgggga
       61 cgctggaggt ggccgacgcg gacgcgctcg atcgtgcgct gctggtgcac ggtgcgcgcc
      121 acacccacct ggcatgcctg tcgccctgga gcctggcgcc gccggagcac ggtcgcgact
      181 gcctggggct gatgcccgcg ctggccgtgg ccgacagcat cgcctggcgc cggccggccg
      241 gcgaattcac cgcggagtgg tactgcgaga cgctgcggtt tgcctgggcg gtggcgctgc
      301 gcaactagcg accccacccc cgggcgtggt aaaggttggc cccccgccgc catccccggg
      361 ccgatggaca cgatcgcaac gctcgcgctg ccggacaccc atggcctcgc cgtgatcgga
      421 ctcgcgctgt tcgcgctggt ggccctgacg cggccgtcgg tgccgcagga atccaccagc
      481 ctgatggtgc tcatgctcct ggtgctgggt ttcgagctgt ttccctacca ggggactgcc
      541 ggcggaatgg gttccggcgc cttcctgtcg atgttcggca acagcgcgct gatcaccgtc
      601 atcgcgttgc tggtggtcgg ccgggccctg gaagtgaccg gcgccctgca gccgctgatc
      661 cggctgatgc tcaaggtctg gaccgggccg cgctcgctgg cgctcatcgt caccctgctg
      721 gcggccgcgt tcgcgtccgc gttcctgtcg aacacgctgg tggtgctggc gctgctgccg
      781 gtgctggtgg gcgcttccac gcggcgtcgt ttcccggcct cgcaggtgct catgcccatg
      841 ggcttcgccg cgctgctggg cggcatgatg accaccatcg gcacctcgac caacctgctg
      901 gtggtgaacg aggccgagcg gctcgggctg ccggatttcg gactgttcca cttcatcctg
      961 ccggccgctg tgggggtggc ggtcgggctg ctgttcctct ggctggtagc gccgcggctg
     1021 ctgcccgagc gcgacgcacc gatccgccag gcggcgccgc gcatattcaa ggcgatgttc
     1081 ttcgtcggcg aggacagctg ggcggtgggg cggacgctgg cggagctgcg cgagcgcacc
     1141 gggcggcgca tgaacgtgtc acgggtgcag cgcggcgacg gcctgcaggt cgcgaagctg
     1201 ccggccatgc gcctgcagtc cggcgaccgc ctgatggtgg ccgatacgcg cgagcgcctg
     1261 aaagagttcg agcgcctgct cggcgtgcgc atgcacagcg agttcgccgg cgaggaggac
     1321 gacggcaccg aggaggaggc ggctggtcag cagctcgccg agctggtggt cacgcccgac
     1381 tcgaccctgt acggcaggac gctctccacg acgcagttcc ggcagcgcta ccggctggtg
     1441 ccgctggcca ttcatcgcgc caccgccgcc ggcaccagcg aggtcaccgg cgatttcgag
     1501 gatgcggtcc tgcaggcggg cgacgtgatt ctcgtccagg gcacgcccga gcggctgcgc
     1561 gagctgcgcg ccagcggcag catgctggtg ctggacgggc gcgtggacct gccgaagacc
     1621 acgcgcgcgc cgctcgccct cgggctgacg gcggcgatcg tgctggcggc cggcgccggc
     1681 tggacgccca tcagcgtgag cgccaccgcg ggcgccgcgc tgctgattgc caccggctgc
     1741 atcgactggc gcgaggtcgc cggcgcgctg tccgcaccgc tgctgctgat catcgtcatg
     1801 agtctcgcgc tggtggaggc cagcgtcggc accggcgccg acctgttcgt cgccgggctg
     1861 ttcctggcgc tgttcgacgg cctgccgccg gccgcggcgc tggcgctggt actgttgctg
     1921 gtgtccgtcg ccggcagcct ggcgcccaat acggcggtcg cgctgctcgc ggtgccggtg
     1981 gcggtgggca tcgcccgcgg cctcggcgcg ccgctggagc cgttcgtgct ggcggtgctg
     2041 ttcggcgcga atctgagctt tgcgaccgcg ctgggttcgc gtcccaacct ggtggtgtcg
     2101 accgcgggcg gctacctggc gcgcgacttc atgcgcgtcg gggttccgtt gaccgtgctc
     2161 gtctggctgg cgatttcgct ggcgctggtg gtgcgctacg cagtgccctg gggctggccg
     2221 gcgggctgag tggctgccac gccggcgggc gggggttgac cggtgtcgcc ggccgggtaa
     2281 catccggccc agcatgaacg cctttctgtc cagcgcacgc ggccactggt ggtggcgtca
     2341 cggatccccg gagggggtcc ggtcgcgcgc agccgactga aacggctcac gccccgaacc
     2401 catctccgga cgccgccggg gatgggttct tggtgacctc ctcccggacg gaccaccgcc
     2461 tcgaggagga ttgatgcact cgcccttcga tatcgccgcc gacctggaca cgcccgtttc
     2521 ggcctacatg aagctggcgc cgttccggcc gcgtttcctg ctggagagcg tggagggcgc
     2581 cgagcgcctc gggcgctatt ccttcatcgg cttcggcgac gcgctgacgg tacggctcga
     2641 ccgcgccggc ctgcacttcg gcggccgcac gcggccggtg gacggcgacc tgctgccggc
     2701 gatgcgtgcg gcgctggcgg cagcgccgac gctggccccg gaggcggggc tgcccttcga
     2761 cggcgggctg gtcggcattg ccgcctacga catcgtgcgc cacttcgagc gcgtgcccgg
     2821 gggcagcggt gactttcccg agggtctctg ggtcgcgccg cgttccctgc tggtgttcga
     2881 tcacctcacc cggcgcgctg cgctgctgca cgcgggcgac gaggcggagc gcctcgccct
     2941 gcggcgcgaa gtggtccagg cgctggccgg cggcgtgccc ttccgccgcg ccggccggca
     3001 tgcgccgccg gcggccagca tgagcgaagc ggagttcctc gccgcggtcg gcaaggcgaa
     3061 gggcaacatc accgccggcg acgtgttcca gctggtgctc tcggtgcgct tctccggccg
     3121 cagcgacatc gcgcccttcg aggcctaccg cgcgctgcgc ctcttgaacc cgtcgcctta
     3181 catgtacttc ctggagctcg acggcctgtc ggtcgccggc tcctcgcccg aggcgctggt
     3241 gaagctggac ggccggcacg cctcgctgcg gcccatcgcg ggcacgcggc cgcgcggcga
     3301 gacccgcgag caggacctgg cgcgcgaggt cgagctgctc gacgacccga aggagaacgc
     3361 cgagcacgtc atgctggtgg acctggcacg caacgacctc ggccgcgtcg ccgtgccggg
     3421 ctcgatccgc gtcgagccga gccgcgccat cgagcgctac agccacgtga tgcacatcgt
     3481 cagcggcgtg cagggcgagc tggaggcggg ccgggacgcc ttcgacctgt tcgcggccac
     3541 cttcccggcc ggcacgctgg tcggcgcgcc gaaggtgcgc gccatggagc tgatcgcgga
     3601 ctacgagccg gtcggccgcg gcctgtacgc cggcacggtc ggctattttt ccaggcgcgg
     3661 cggcatggac caggcgatcg ccatccgcac gctggccttt cgcggcgacg agtacagctt
     3721 ccaggccggc gccggcatcg tcgcggacag cgtgccgctg aatgaatacc gcgaggtgct
     3781 ggccaagagc gcgatcctgc ggcgcgcgct ggaaatggcg gaggagggac tgtgagcgcg
     3841 cgcgtgctga tgatcgacaa ctacgactc
//
LOCUS       DAOMPG010000227         3848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9806_length_3848_cov_4.934353, whole genome shotgun sequence.
ACCESSION   DAOMPG010000227 DAOMPG010000000
VERSION     DAOMPG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_3848_cov_4.934353"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLS34_10815"
     CDS             complement(<1..804)
                     /locus_tag="PLS34_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005175648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP87992.1"
                     /translation="MTGSKVYPVPADFAAAAHCNKEKYAQMYAASVANPEKFWGEHGK
                     RIDWIRPYTKIKDVSYDRHHLHIRWFEDGTLNASANCLDRHLAERGDQTAIIWEGDDP
                     ADSKHISYRQLYEQVCRLANGLKSLGIGKGDMVTIYMPMIPEAAVAMLACARIGAPHS
                     VVFGGFSPESVAGRVEDCDSKLIITADEGLRGGRKVPLKANVDRALEQHKLDSVANVV
                     VIRRTGGKVGWKEGRDVWYEELVKDQPAECPPAEMGAEDTLFILYTSGST"
     gene            1190..2281
                     /locus_tag="PLS34_10820"
     CDS             1190..2281
                     /locus_tag="PLS34_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRP87993.1"
                     /translation="MNKPLSSGLLAAAALALVPMSASAWSPKLEVSDTTWVQLGYLHQ
                     FWYQSVDDGAASGDDPSNEFFTRRSRVMLLGQATENIHFFLNYDAAAGTGSGDRQDPV
                     LTDAMIDYRFSPAFNVMVGRQLVPFSIDNNTSAVTLTGIDYPTRALVNQPSTASGAFW
                     RDDGITVRGLLADGRFDYRVGYFAGDRSGTNNPERDGRITGMVMINLASPQGGWFFNQ
                     NSLGALDVLSFGIGYDQMSRSAIDDHKAMSLFVTAEKKLGAGHLSANAAYYDWEGVGG
                     PGNAFYNGGSTSVLALAFLPTGSKWQPVIRWQNQDPDDGDKLDTIGLGLSYYISGHRA
                     NIKAEYSIDDTRIGGQEKDAFRVQAQLFF"
     gene            2444..3229
                     /gene="phbB"
                     /locus_tag="PLS34_10825"
     CDS             2444..3229
                     /gene="phbB"
                     /locus_tag="PLS34_10825"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRP87994.1"
                     /translation="MLHRVQALIDTRENGMTGRTALVTGGTGCIGRQICETLAAAGYR
                     VAANGHPADAEAAARWQQEAAGRGLDIHLEAFDAGDYEATLAGVAAVEAAVGPVDVLV
                     NAAGITRDGRLVNLDPEDWKAVVRTNLDSVYNATKAVIGGMIGRSFGRVVNISSVNGQ
                     RGQFGQANYSAKAGVHGFTMAMAREVARKGVTVNTVSPGYIESPLIMKVPEPIRESIR
                     ESIPAGRFGKPEEIARAVLFLASDDAAYITGADLSVNGGYHMD"
     gene            3274..3756
                     /gene="phaR"
                     /locus_tag="PLS34_10830"
     CDS             3274..3756
                     /gene="phaR"
                     /locus_tag="PLS34_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRP87995.1"
                     /translation="MKERVIRKYANRRLYDPSESRHLTLDEVRELVVAGEKIRVEDAK
                     TGEDLTRSILLQIIVEREEAGRPLLSAELLEQLIRFYGGAMQDFLSTYLERSVGAFVD
                     QQQSFQDRILNMMKDTPLGGMSQLAEQNLAAWKELQQGLFSAGSAQSQKKKPGKKSHK
                     "
BASE COUNT          671 a         1260 c         1245 g          672 t
ORIGIN      
        1 ggtcgagccg gaggtgtaga ggatgaacag cgtgtcctcg gcgcccatct cggccggcgg
       61 gcactcggcg ggctggtcct tgaccagttc ctcgtaccag acgtcgcggc cttccttcca
      121 gcccaccttg ccgccggtgc gcctgatgac caccacgttg gcgacgctgt cgagcttgtg
      181 ctgctcgagc gcgcggtcga cgttggcctt cagcggcacc ttgcggccgc cgcgcaggcc
      241 ctcgtcggcg gtgatgatca gcttcgaatc gcagtcctcg acgcggcctg ccaccgactc
      301 tggcgagaag ccgccgaaca ccacggagtg gggcgcgccg atgcgcgcgc aggccagcat
      361 cgccaccgcc gcctcgggga tcatcggcat gtagatggtg accatgtcgc ccttgccgat
      421 gcccagcgat ttcaggccat tggccagccg gcagacctgc tcgtagagct ggcgatagct
      481 gatgtgcttg gagtccgccg ggtcgtcgcc ctcccagatg atggcggtct ggtcgccgcg
      541 ctcggccagg tggcggtcca ggcaattggc gctggcgttg agggtgccgt cctcgaacca
      601 ccggatgtgc aggtggtggc ggtcgtagga cacgtccttg atcttggtgt acggccggat
      661 ccagtcaatc cgcttgccgt gttcccccca gaacttttcc gggttcgcca cggacgcagc
      721 gtacatctgc gcgtactttt ccttgttgca gtgggcggcc gcggcaaaat cggccggcac
      781 cggatagacc ttggatccgg tcatcgattc ctccttatgt ccccgggtcc ccggacgggt
      841 ttcgcgggac gctggcttgg ttttccgagt cttggttcca cccctcgggg agtacattag
      901 actttggtcg cagcggcgcc gggggcgccg tctcctagct tagcgcaact ggggacggcg
      961 tcccgcccag ccgtcgctcc cggtgggcgg acgcactttt gttgcttttt taccacgcaa
     1021 taattccgca atgtttcggt tttccccgat aaaagatgcg ccggggtagc agaaacttag
     1081 actttcagct aataccaata cagtgccgcc gtgggtgatc atcggcggcg cgcgttgcct
     1141 ccagaccaca atggatgacg agtcacatac cgggagaata atgatgcgaa tgaacaaacc
     1201 cctctccagc ggcctgctgg cggcggccgc actggcgctg gtgccgatgt cggcgagcgc
     1261 ctggagtccg aagctcgaag tcagcgacac cacctgggtg cagctcggct acctgcacca
     1321 gttctggtac cagagcgtcg atgacggcgc cgccagcggc gacgacccga gcaacgagtt
     1381 cttcacccgc cgcagccgcg tcatgctgct cggccaggcg accgagaaca tccacttctt
     1441 cctgaactat gacgccgccg ccggcaccgg cagtggcgat cgccaggatc ccgtgctgac
     1501 cgacgcgatg atcgactatc gcttctcgcc ggccttcaac gtgatggtgg gccgccagct
     1561 ggtgcccttc tcgatcgaca acaacacctc ggccgtcacg ctgaccggta tcgactaccc
     1621 gacccgcgcg ctggtcaacc agccgagcac ggccagcggc gccttctggc gcgacgacgg
     1681 catcacggtg cgcggcctgc tggccgacgg ccgcttcgac tatcgcgtcg gttacttcgc
     1741 cggcgaccgc agcggcacca acaatcccga gcgcgacggc cgcatcaccg gcatggtcat
     1801 gatcaacctc gcctccccgc agggcggctg gttcttcaac cagaacagcc tcggcgcgct
     1861 ggacgtgctg agcttcggta tcggctacga ccagatgagc cgcagcgcga tcgacgacca
     1921 caaggcgatg agcctgttcg tgacggcgga aaagaagctg ggcgccggcc acctgtcggc
     1981 gaatgccgcg tactatgact gggaaggcgt gggcggcccc ggcaacgcgt tctacaacgg
     2041 cggctcgacc tccgtgctgg cgctggcctt cctgcccacg ggcagcaagt ggcagccggt
     2101 catccgctgg cagaaccagg atcccgatga cggcgacaag ctcgacacca tcggcctggg
     2161 cctgagctat tacatcagcg gccatcgcgc caacatcaag gccgagtact cgatcgacga
     2221 tacccgtatc ggcggtcagg agaaggacgc cttccgcgtt caggcgcagc tgttcttcta
     2281 ggcctccggg cagagatcac cggccatccc ggccggcaag gcgggcggcc ttcgggccgc
     2341 ccgctttcgt ttgcgccgct gcaaaaacgt ttcgttcgct gcctgcgtta tgtccggtga
     2401 ctggcgcatg ttgggttttt gcattgcaca acaaaaacgc gctatgctgc accgcgtcca
     2461 ggcactcatc gacacgcggg agaacggcat gacgggacgg actgcattgg tgaccggtgg
     2521 cacgggctgc atcggcaggc agatctgcga aacgctcgcg gcggccggct atcgcgtggc
     2581 ggccaacggc cacccggcgg atgccgaggc cgcggcccgg tggcagcagg aggccgccgg
     2641 aagagggctg gacatccacc tcgaggcctt cgacgcgggc gactacgagg ctacgcttgc
     2701 cggggtggcc gccgtcgagg ccgcggtggg tcccgtggac gtgctggtga atgccgccgg
     2761 gatcacccgc gacgggcgcc tggtgaacct cgatcccgag gactggaagg cggtggtccg
     2821 caccaacctc gacagcgtct acaacgccac caaggcggtg atcggcggca tgatcggtcg
     2881 cagcttcggc cgcgtggtga atatctcctc ggtcaacggc cagcgtggcc agttcggcca
     2941 ggccaactac tccgccaagg ccggcgtgca cggtttcacc atggcgatgg cccgcgaggt
     3001 ggcgcgcaag ggtgtcaccg tgaacacggt atcgcccggc tacatcgagt cgccgctgat
     3061 catgaaagtg cctgagccga tccgcgagag cattcgcgag tccatcccgg ccggacgttt
     3121 cggcaagccg gaggaaatcg cccgcgcggt gctgttcctg gcctcggacg acgccgccta
     3181 tatcaccggc gccgacctgt cggtgaacgg cggctaccac atggactgag gccggcgctt
     3241 cactgtcggg ccgtgcggct gcattatggg cccatgaaag aacgcgtcat acggaaatac
     3301 gccaatcgcc gcctctacga tccctcggag agtcgccatc tcaccctgga cgaggtccgc
     3361 gagctggtgg tcgctggtga gaagatccgc gtcgaggacg ccaagaccgg cgaagacctg
     3421 acgcggagca tcctgctgca gatcatcgtc gagcgcgagg aagccgggcg gccgctgctg
     3481 tcggccgagc tgctggagca gctgatccgc ttctacggcg gcgccatgca ggatttcctg
     3541 tcgacctacc tcgagcgcag cgtcggcgcc ttcgtcgacc agcagcagag cttccaggac
     3601 cgcatcctga acatgatgaa ggacaccccg ctgggcggca tgagccagct ggccgagcag
     3661 aacctggccg cctggaagga gcttcagcag ggcctgttca gcgccggttc cgcgcaatcg
     3721 caaaagaaga agcctgggaa aaagagccat aagtagctgt tttatttaaa taggcataat
     3781 ccacaagcag gttgtttttg cattgcaaaa agacttctgc taaggttcgc ggcaagtcgg
     3841 caagcgcc
//
LOCUS       DAOMPG010000228         3848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9809_length_3848_cov_4.155286, whole genome shotgun sequence.
ACCESSION   DAOMPG010000228 DAOMPG010000000
VERSION     DAOMPG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_3848_cov_4.155286"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLS34_10835"
     CDS             <1..492
                     /locus_tag="PLS34_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HRP87996.1"
                     /translation="PLAKAMDRVLVPFLVFGALTGVITYVLGLMVWPADTLQTTPLGR
                     NHMMAATWSMFYWGAVLALRWWVGEKAWEGLTNRLIMLGLGALGGGLLAITGTLGGHL
                     HGAPSFVSSVLRMVGWEVYASFYFPNWVLVLLAVVIVVMPVVAIVTLRGRAARLSTEG
                     GAA"
     gene            489..815
                     /locus_tag="PLS34_10840"
     CDS             489..815
                     /locus_tag="PLS34_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRP87997.1"
                     /translation="MRPVLLLFVLLLLALGAMRFAQAVPPGLVLEFEGARQGKVTFQG
                     DVHSGTGMYCADCHMAIFDVSRSAEISRRDHNEEVFCFTCHNGETAFAARRNCGNCHL
                     EEDGET"
     gene            complement(788..1780)
                     /locus_tag="PLS34_10845"
     CDS             complement(788..1780)
                     /locus_tag="PLS34_10845"
                     /inference="COORDINATES: protein motif:HMM:NF026213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRP87998.1"
                     /translation="MLEETLGRKVKVAAILLTHWHYGNGIGAWLDEGAEVWGHEHLDR
                     NFSASTSISVLSGTYQARAAAQFGVFHPQSGPDAFPNMLGFTPEKLTAGSSYQPPTRL
                     FPDGRVIDLVVAGEPVQVAPLRTDASDSVGFYFPRWRMLVTNVMPCGAVFNIYTLRGG
                     SYRNPAVMIQDARWFESKNAEIHLDIHYPTLKGEDVVRDAVQRFLDQVQLTHDQTLRL
                     IAHGRDGRQAAEEVYIPHHLRGDWENYGQVESHVRQVYNGTMGWFGGDVYDINPLSGN
                     EEAARTVQMMGGPAAVIELHPRGGSILGAGRGVDKGPSRTRTPPTGQVSPSSSR"
     gene            1779..2399
                     /locus_tag="PLS34_10850"
     CDS             1779..2399
                     /locus_tag="PLS34_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP87999.1"
                     /translation="MARISVAAGVWSHAVVGEYDTAHRYFLRELEISRANFGEWHMRT
                     ALAYNNLGMISRRMGKLERALEHLQRSLEIIRESGAGQHQIPGLYLNMGNTWEDLGQL
                     EVAAEHFEAGLAHEGLAEGDRRIHSFLLNNFGSLLVNLGEDERGAVMVSLATALSASR
                     HRAILAMSGGSLQRCRALAYGSMEGYKPPPSATARSLVGSMTVSDL"
     gene            complement(2333..3046)
                     /locus_tag="PLS34_10855"
     CDS             complement(2333..3046)
                     /locus_tag="PLS34_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP88000.1"
                     /translation="MAKSPDQSSPAAFPLRGWFADTHPGFRQAIFAMARPKSYAAGHV
                     IYKAGDAGREFFGVRSGVVTMQGRFTHPDAVMLHMLRAGDWFGTAPVLAGRSRRATAV
                     ARTDVELLLVPGGELQALLRHHPEWIVDLARDVVYFLDVALQGSADLLIRDAAARCAA
                     VLLRLAGRRWASEPDADLPAEIPVSQSDLAMLCNLSRSTVSRVVKELAGHRLVTLGYK
                     SLTVIDPTRLRAVADGGGL"
     gene            3206..>3848
                     /locus_tag="PLS34_10860"
     CDS             3206..>3848
                     /locus_tag="PLS34_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP88001.1"
                     /translation="MPFRKPNILTLLAACALGASPAASAADAASGSRESAPTASWHSQ
                     SLVTETVTGPNGAITTRTALSVNPRLGVTEAKIAEQVAEGVYALRGWGIAHSFAIEAA
                     DGWIIVDTGDSTQAATEMREALERAVGGKIKVAAILLTHWHYADGTAAWLDQGTEIWG
                     HEHLDRNRIASGGVSVLGGYLNSRATAQFGVFHPATGPDAFPNLLSFTPEKFLV"
BASE COUNT          616 a         1252 c         1331 g          649 t
ORIGIN      
        1 ccgctggcga aagccatgga tcgggtgctg gtgccgttcc tggtgttcgg tgcgcttacc
       61 ggtgtgatca cctacgtgct cggcctgatg gtgtggccgg ccgataccct gcagaccacg
      121 ccgctggggc gcaaccacat gatggccgcc acctggtcga tgttctactg gggcgcggtg
      181 ctcgcgctgc gctggtgggt cggtgaaaag gcctgggagg gtctcaccaa ccggctgatc
      241 atgctgggac tcggtgcgct gggcggcggc ctgctggcga tcaccggcac gctgggcggc
      301 cacctgcacg gcgcgccgtc cttcgtcagc agcgtgctgc ggatggtcgg ctgggaggtc
      361 tatgcctcct tctacttccc gaactgggtg cttgtcctgc tggcggtggt catcgtggtg
      421 atgccggtgg tggccatcgt cacgttgcgg ggacgggccg cgcggctgag caccgaagga
      481 ggtgcggcgt gagaccggtg ctgttgctgt tcgtgctgtt gctgctggcc ttgggcgcca
      541 tgcgcttcgc gcaggcggtg ccgcccggcc tcgtgctcga gttcgagggt gcgcgccagg
      601 gcaaggtgac cttccagggt gacgtccatt cgggcaccgg catgtattgc gccgattgtc
      661 acatggccat cttcgacgtg agccgctcgg ccgagatcag ccgccgcgac cacaacgagg
      721 aggtgttctg ctttacttgc cacaacggcg agacggcctt cgccgcgcgt cgcaactgcg
      781 gcaactgtca cctcgaggaa gatggcgaga cctgaccggt cggaggggtc cgagtccggc
      841 tcggaccctt gtccacgccg cgcccggccc ctagaatcga gcccccacgc gggtgtagtt
      901 caatgacggc ggcggggcca cccatcatct ggacggtacg cgccgcttcc tcgttcccgg
      961 acagcggatt gatgtcgtac acgtcgccgc cgaaccagcc catcgtgccg ttgtagactt
     1021 ggcggacgtg gctttcgacc tgcccgtagt tctcccagtc gccacgcagg tggtgcggga
     1081 tgtagacctc ttcggccgct tgtcgaccgt cccggccgtg cgcgatcagg cgcagggttt
     1141 gatcgtgggt caactggacc tggtcgagaa agcgctggac cgcgtcgcgc accacgtcct
     1201 ctcccttgag ggtggggtaa tgaatgtcga ggtgaatttc cgcgttcttc gactcgaacc
     1261 agcgcgcgtc ctggatcatg accgccggat tccgatacga gccgccgcgc agcgtataga
     1321 tgttgaagac ggcgccacac ggcatgacgt tggtcacgag catgcgccat cgcgggaaat
     1381 agaaaccaac gctgtcggaa gcgtccgtcc ggaggggagc gacctgcacc ggttcgccgg
     1441 ccacgaccaa gtcgatgacg cgtccgtccg ggaagagccg cgtgggcggc tggtagctgg
     1501 atccggccgt cagtttctcc ggcgtgaaac cgagcatgtt ggggaacgcg tcgggaccgc
     1561 tctggggatg gaagacgcca aactgggcgg ccgcccgcgc ctggtaggtg ccgctcagga
     1621 cactgatgct ggtcgaggcg ctgaagttgc ggtccaggtg ctcgtgtccc cagacctccg
     1681 cgccctcgtc gagccacgcg ccgatgccgt tgccgtagtg ccagtgggtc agaagaatcg
     1741 cggccacctt gactttgcgg ccgagcgtct cctcgagcat ggcgcgcatc tccgtggcgg
     1801 ccggcgtgtg gtcacatgcg gtggtcggcg agtacgacac ggcgcaccgc tatttcctgc
     1861 gtgaactgga gatcagccgg gcgaacttcg gcgaatggca catgaggact gcgctcgcct
     1921 acaacaacct cggcatgatt tcgcggcgga tgggcaagct cgagcgggcc ctggagcatt
     1981 tgcagaggag cctggaaatc attcgcgaga gcggtgccgg ccagcaccag atcccgggcc
     2041 tgtacctcaa catgggcaac acctgggaag acctcgggca gctggaggtg gccgccgagc
     2101 acttcgaggc ggggctggcg catgaaggcc tggccgaggg ggaccggagg attcacagtt
     2161 tcctgctgaa caatttcggc agcctgctgg tgaacctggg cgaggacgaa cggggcgcag
     2221 tcatggtctc attggcgacc gccttgtctg cctcgaggca cagggccatt ctagccatga
     2281 gcggcggcag ccttcaaaga tgccgcgccc tggcctatgg gtcgatggag ggctacaagc
     2341 cgccgccgtc ggcaacggcg cgcagtctcg ttgggtctat gactgtcagc gacttgtagc
     2401 cgagggtcac cagtcgatgg cccgcgagct ctttcacgac gcggctgacg gtactccggg
     2461 acaggttgca gagcatggcg aggtccgatt gcgacaccgg gatctcggcg ggcaggtctg
     2521 cgtccggctc cgacgcccag cgccgcccgg ccaggcgcag caggacggcc gcgcagcgcg
     2581 ccgcggcatc ccggatcagc aggtcggcag atccctgtaa tgccacgtcc aggaaataca
     2641 ccacgtcgcg cgcgagatcg acaatccact ccgggtggtg ccgcagcagc gcttgcagct
     2701 ccccgccggg cacgagcagg agctccacgt cggtgcgcgc cactgcggtg gcgcgacggc
     2761 tccggcccgc cagcaccggc gcggtgccaa accagtcgcc ggcccgcagc atgtgcagca
     2821 tcaccgcatc cggatgggtg aacctgccct gcatggtgac tacgcctgag cggacgccga
     2881 aaaactctcg ccccgcatcg cctgccttgt agatcacgtg gccggccgcg taggacttcg
     2941 gccgggccat ggcgaagatc gcctgcctga agccgggatg ggtgtctgcg aaccagccgc
     3001 ggagcgggaa ggcggctggg ctgctctggt caggggactt ggccatgcgg actcctcccc
     3061 ggaagttgcc gggtggcgcg gcgattgtcc cattttgcgc aatgcatgtc gccgtgccgt
     3121 gctatctgag tccgcaccga acggtgcatc gacaccaacc cgatgctgaa cgccgggccc
     3181 catgccgcat gacgaggtag ccgcaatgcc cttccgcaag ccgaacattc tcaccctcct
     3241 ggctgcctgt gcgctgggag caagccctgc cgccagtgct gccgacgccg cgagcggcag
     3301 ccgggagtcc gcgccgacgg catcctggca cagccagtcc ctggtcaccg agaccgttac
     3361 agggcccaac ggggccatca cgacccgcac ggcgctcagc gtcaacccgc ggctgggcgt
     3421 gaccgaagcg aagatcgccg agcaggttgc tgaaggcgtg tacgcgctgc gcggctgggg
     3481 catcgcgcac agcttcgcca tcgaagcggc ggacgggtgg atcattgtcg acaccggcga
     3541 ctccacccag gccgcgaccg aaatgcgcga ggcgctcgag cgagccgtcg gcggcaagat
     3601 caaggtcgcg gcgatcctgc tgacgcactg gcactacgca gacggcaccg ctgcgtggct
     3661 ggaccagggc accgagatct ggggtcacga gcacctggac cgcaaccgga tcgcctccgg
     3721 cggcgtcagc gtcctgggcg gatacctcaa ttcgcgggcc actgcgcagt tcggcgtgtt
     3781 ccacccggcg acgggccccg acgcgtttcc gaacctgctc agcttcactc ccgagaagtt
     3841 cctggtgg
//
LOCUS       DAOMPG010000229         3840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9831_length_3840_cov_4.047556, whole genome shotgun sequence.
ACCESSION   DAOMPG010000229 DAOMPG010000000
VERSION     DAOMPG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9831_length_3840_cov_4.047556"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PLS34_10865"
     CDS             <1..179
                     /locus_tag="PLS34_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRP88002.1"
                     /translation="PKDVVNRLRTSDGKPLYLVEADDGSYRLTPYDPEFEAKMARAED
                     IISRYRNTLHALAK"
     gene            176..565
                     /locus_tag="PLS34_10870"
     CDS             176..565
                     /locus_tag="PLS34_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRP88003.1"
                     /translation="MNEPAWLDARDVIFLHERLIGLYGGPGGLRDRGLLESALGRARQ
                     LRAYDERADIIDLAAASTAGIVLNHPFVDGNKRTGFLVGVLFLELNGFRFTADEAAAT
                     RAVIELAAGTLDAVGYAAFLRANATRE"
     gene            782..>3840
                     /locus_tag="PLS34_10875"
     CDS             782..>3840
                     /locus_tag="PLS34_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88004.1"
                     /translation="MRRSWAILLLLLGLPGPAALAQYQALHIDETQLPESEQAARNAA
                     SYAAILAQIAIHNASIPLGFYPGPTVSVPAEIAGLAADNTLKFTKAFLRAPPDVAVLP
                     NVDGCRFEFELPQATTEFDSFLGLWPRLGDQRLFLPNDPPIDFGILGVPEIVHANTNV
                     LLSVDSPSAGITRDAGEEAQWVVAGPGVHAVEWRADTQWYPVWDALVPTITTAVMLGM
                     EFKFSKHIKKLAKARSDTLELASTKGDAKRLAALLNDLNRRIQLFERFQWRLLGGEVL
                     FAAGIPLTGELIVEKFFDGKTTVSRSRNQWLTVYKVEPPVIGTSQSNPSFEATDVGGA
                     RTSRYLDDLTAMIDISDPCGAELSLVNDAPALLPLGDTLVTWTVRDQGPVAPGSDTDG
                     DGSPDDLPYNSRTVTQLVTIADTQAPILVPPPGVVIESDAGIDIDDQALGQPLVVDLA
                     DLHPLVSNATDAPGGIVPPDTRAVVTWSAVDGSGNASSADQLVTVKTPGTNTAPLAFG
                     TSADTLTSQAVDIVLSGADADLLPLAGDESGAPGIPDPLQFRIVERPQHGEFIAPLLP
                     YFIEDYRTDTTGVLGDDVAFAQAENQLAWLDQQYCQDDLEIPIEFVFKPLFVQVTDEG
                     ESYFLDHFAFCPPFNEPADEALRISRWAPDGSFIGHVEYNPSGGVHTSSFVLDRNGNI
                     HYLLSFGVNEFTLQLCPGDFTGTTTSCTNGYNFPDNIDGFGVSASTFALLDHREVGGS
                     GEPLIYLTDRRRVNVYQGQLFKGTLSNDAGLPDFITQPTSCQPIPGGGDRQGYGMALD
                     SEGAFYIADTCGHRIHKFAASTLDEDGQLVPGEYVGWLGQCSGSSNLACDETLQATKG
                     FSCTDATCTAAGDRSGAGIGQLHGPVYLAMDPNDVLYVADYVNQRIQRFAPDGTFAGP
                     AQSTGNGINATTDGAFVLGNMGPPRHVTVNSQQFFVVDRAERFVHVFETSPFKDISSD
                     SATVSYVSNFDFHSDTDTFAFVVNDGLADSAPAQVSVAVARNYRQPLP"
BASE COUNT          656 a         1364 c         1224 g          596 t
ORIGIN      
        1 tgccgaagga cgtcgtcaac cggttgcgca ccagcgatgg caagccgctc tatctggtcg
       61 aggccgacga tggcagctat cgcctgacgc cctacgaccc ggaattcgag gcgaaaatgg
      121 ccagggccga agacatcatc agccggtacc gcaacacgct gcacgcgctc gcgaagtgaa
      181 tgagccggcg tggctcgacg cccgcgacgt cattttcctg cacgagcgct tgatcggcct
      241 gtacggtggt cctggcgggt tgcgtgaccg gggcctgctc gaatccgctc tcggtcgagc
      301 gcgacagctg cgtgcgtatg atgagcgcgc cgacatcatc gacctggcgg cggccagcac
      361 ggccggcatc gtcctcaatc atcctttcgt cgacggcaac aagcgcaccg gcttcctggt
      421 cggcgtgctg ttcctggaat tgaatggctt tcgattcacg gccgatgagg ccgcggctac
      481 gcgggcggtg atcgagcttg ccgcggggac gctggacgct gtgggctacg ccgcattcct
      541 gcgcgcgaac gcgaccaggg agtgaattcc cctgctgcgc gtcaccgcgg tgcagcataa
      601 aaaaatgttg cggacgtggt gagcacggtt atagggttgc actgggagaa caaccaacaa
      661 gaaactcgac agccacctgc cgactaatgg atgagcaagc cggatggctc gatctcgatg
      721 agggggaggt cgggatggct tgccggcaga ccggccgatt tgcagagcgt tcgtgcctcc
      781 ggtgaggcga tcatgggcca tcctgctcct gctgctcggc ctgcccgggc ccgccgcact
      841 ggcgcagtac caggcgctgc acattgacga gacccagctg ccggagagcg agcaggccgc
      901 gcgcaatgcc gcctcctacg ccgccatcct cgcgcagatc gccatccaca acgcatccat
      961 cccgctcggc ttctacccgg ggccgacggt ctcggtcccg gcggaaatcg ccgggctggc
     1021 ggccgacaac acgctgaaat tcaccaaggc cttcctgcgc gccccgccgg atgtggcggt
     1081 actgccgaat gtggacggct gccgcttcga attcgagctg ccgcaggcca ccactgagtt
     1141 cgacagcttc ctcgggctct ggccgaggct cggcgaccag cgactgttcc tgccgaacga
     1201 tccgccgatc gacttcggca ttctcggcgt gcccgaaatc gtgcacgcca acaccaacgt
     1261 gctgctcagc gtcgacagcc cgagcgccgg catcacccgc gatgccgggg aggaggccca
     1321 gtgggtcgtc gcgggcccgg gcgtccatgc ggtcgagtgg cgcgccgaca cccagtggta
     1381 cccggtgtgg gacgcgctgg tgccgaccat cacgacggcg gtgatgctgg gcatggagtt
     1441 caagttctcg aagcacatca agaagctggc gaaggcccgc agcgacaccc tggagctggc
     1501 ctcgaccaag ggcgacgcca agcgactggc ggcgctgctc aacgacctga accggcgcat
     1561 ccagctgttc gagcgcttcc aatggcggct gttaggcggc gaggtgttgt tcgcggcggg
     1621 catcccgctg accggtgagc tgatcgtcga gaagttcttc gacggcaaga ccaccgtgtc
     1681 gcgctcgcgc aaccagtggc tcaccgtgta caaggtggag cccccggtaa tcggcaccag
     1741 ccagtccaac ccgagcttcg aggccactga cgtcggcggc gcgcgcacca gccgctacct
     1801 cgacgacctg acggcgatga tcgacatcag cgacccctgc ggcgccgagc tcagcctggt
     1861 caacgacgcg ccggcgctgc tgccgctcgg cgacaccctc gtcacctgga cggtgcgcga
     1921 ccagggcccg gtcgctcccg gcagcgacac cgacggcgac ggctcgccgg atgacctgcc
     1981 ttacaactcg cgcaccgtca cccagctggt aaccatcgcg gacacccagg cgccgatcct
     2041 ggtgccgccg cccggcgtgg tgatcgagag cgacgccggc atcgacatcg acgaccaggc
     2101 gctcggccag ccgctggtcg tggacctggc ggaccttcat ccgctggtca gcaacgccac
     2161 cgacgcgccg ggcggcatcg tcccgccgga cacgcgcgcg gtggtgacct ggagcgcggt
     2221 cgacggctcc ggcaatgcct cgagcgcgga ccagctggtg accgtcaaga cccccggcac
     2281 caacaccgcg ccgctcgcgt tcggcacgag cgcggacacg ctgacctcgc aagccgtcga
     2341 catcgtgctg agcggcgcgg atgccgacct cctgccattg gcgggggacg agtccggcgc
     2401 acccgggatt cccgacccac tgcagttccg tatcgtcgag cggccgcagc acggcgagtt
     2461 catcgcgccg ctgttgccgt acttcatcga ggattaccgc accgacacca ccggcgtgct
     2521 cggcgacgac gtggccttcg cccaggcgga gaaccagctg gcctggctgg accagcagta
     2581 ctgccaggac gacctcgaga tcccgatcga gttcgtgttc aagccgctgt tcgtgcaggt
     2641 gaccgacgag ggcgaaagct attttctcga tcatttcgcg ttctgcccgc cattcaacga
     2701 gccggccgac gaggcgctgc gcatctcccg ctgggcaccc gatggctcgt tcatcggcca
     2761 cgtcgagtac aacccgagcg gcggcgtgca cacctcgtcc ttcgtgctgg accgcaacgg
     2821 caacatccac tacctgctga gcttcggcgt caacgagttc accctgcagc tctgccccgg
     2881 cgacttcacc ggcacgacca cctcctgtac caacggctac aacttccccg acaacatcga
     2941 cggcttcggc gtgtccgcca gcactttcgc gctgctggac caccgcgagg tcggcggctc
     3001 cggcgagccg ctgatctacc tgaccgaccg gcggcgggtg aacgtctacc agggccagct
     3061 gttcaagggc accctgagca acgacgcggg gctgccggac ttcatcacgc agccgaccag
     3121 ctgccagccg atccccggcg gcggcgaccg ccagggctac ggcatggcgc tcgactccga
     3181 gggtgcgttc tacatcgccg acacctgcgg ccaccgcatc cacaagttcg ccgcctcgac
     3241 gctcgacgag gacggccagc tcgtgcccgg cgagtacgtc ggctggctgg gacagtgcag
     3301 cggcagcagc aacctggcct gcgacgagac gctgcaggcg accaagggct tcagctgcac
     3361 cgacgccacc tgcaccgcgg ccggcgaccg ctccggtgcg ggcatcggcc agctgcatgg
     3421 cccggtctac ctggcgatgg acccgaacga cgtgctgtac gtcgccgact atgtcaacca
     3481 gcgcatccag cgcttcgcgc cggacggcac cttcgcggga ccggcgcagt ccaccggcaa
     3541 cggcatcaac gccaccaccg acggcgcctt cgtgctcggc aacatgggcc cgccgcgcca
     3601 cgtcacggtc aactcacagc agttcttcgt ggtcgatcgg gcggagcgct tcgtgcacgt
     3661 gttcgagacc tcgcccttca aggacatcag cagcgactcg gcgacggtca gctacgtttc
     3721 caatttcgat ttccacagcg ataccgacac cttcgccttc gtggtcaacg acggtctcgc
     3781 cgacagtgcg ccggcacaag tctccgtcgc ggttgcgcgc aactaccgcc agccgctgcc
//
LOCUS       DAOMPG010000230         3814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9923_length_3814_cov_4.206172, whole genome shotgun sequence.
ACCESSION   DAOMPG010000230 DAOMPG010000000
VERSION     DAOMPG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9923_length_3814_cov_4.206172"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..900
                     /locus_tag="PLS34_10880"
     CDS             76..900
                     /locus_tag="PLS34_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRP88005.1"
                     /translation="MARPGRSRRRVAAARRTGFFSRESWRLPKLPAWPWQTLATGALT
                     AAALGAGLWGAGLLLDRPIRQVIVQGPFERVSVLQIEAAVGELRNTGFLSVRLDAVRA
                     KIVAIDWVDDAVVRRRWPAEVEIVVREQVPAARWGDSGLLNTRGELFVRDARHMPPEL
                     PQLSGPEGTEQLVARRYLDARATLAAVGLRLRALELDARGSWRIELSNGLQLRLGRES
                     FERRLRRFARVAAPLLGPRAGEVAYVDMRYSRGFAVGWREAAPGNPAMKEGLTHDG"
     gene            893..2128
                     /gene="ftsA"
                     /locus_tag="PLS34_10885"
     CDS             893..2128
                     /gene="ftsA"
                     /locus_tag="PLS34_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRP88006.1"
                     /translation="MAKRNARDTIVGLDIGTSKVVAIVGEVTEEGGLEVIGIGSHPSR
                     GLKKGVVVNIESTVLSIQRAVEEAELMAGCEISDVYAGIAGSHVRSLNSHGIVAIRER
                     EVTPGDVERVIDAARAVAIPADQKILHIIPQEFLIDHQEGIREPIGMSGVRLEAKVHM
                     VTGAESAAQNIIKCVQRCGLNVVDIVLEQLASSHAVLSEDEKELGVCLVDIGGGTTDI
                     AVFSGGAIRHTAVIPIAGDQVTNDIAVSMRTPTQYAEEIKIRYACALSQLANPDETIE
                     VPSVGDRPPRRLARQTLAEIVEPRYEELFTLIRNEVARSGFDDMIAAGVVLTGGSAKM
                     EGAIELAEEVFHLPVRLGVPHGVTGLADVVRNPIHATGVGLLQYALHEMQQRQGESPM
                     SGGIKDVWARMKAWFQGNF"
     gene            2168..3373
                     /gene="ftsZ"
                     /locus_tag="PLS34_10890"
     CDS             2168..3373
                     /gene="ftsZ"
                     /locus_tag="PLS34_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRP88007.1"
                     /translation="MFELVDGYNTNAVIKVIGVGGGGGNAVSYMMECGIDGVEFICAN
                     TDSQALKNSRVKTAIQIGCNITKGLGAGANPDLGRQAAMEDRDRIQESIEGADMLFIT
                     AGMGGGTGTGAAPIVAQVAKELGILTVAVVTKPFGMEGRKRLDIAEMGITELSKYVDS
                     LITIPNQKLLTVLGKQTTLLDAFKSANQVLQGAVQGIAELITRPGLINVDFADVRTVM
                     AEMGMAMMGTGRGTGEDRAREAAEAAISSPLLEDINLEGAKGILVNVTAGLDLAIGEF
                     DEVGNVVKERAHEDATVVVGTVIDPAMEGEVRVTVVATGLGGVLRQREPDLRVASSNT
                     RQSRVRRSAGIGPVNYSDLESPPVVRRRADEPEDMVEAPKRAVGQSFEYRSDSELLDI
                     PAFLRRQAD"
     gene            3519..>3814
                     /locus_tag="PLS34_10895"
     CDS             3519..>3814
                     /locus_tag="PLS34_10895"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRP88008.1"
                     /translation="MLRQRTLKNTIRATGVGLHTGEKVLMTLGPAPVNTGIVFRRTDL
                     DPPVEIRADGMHVGETTLGTTLVRDGVRVATVEHLMSALAGLGIDNAIVELSAQ"
BASE COUNT          616 a         1278 c         1392 g          528 t
ORIGIN      
        1 tcgatttcga cgagctggcc tggcgcgtgc tggagaccag tttcgcgccg cgcgggacca
       61 acacggaggt gcgcgatggc gcgaccgggt aggtccaggc gccgtgtcgc ggccgcccgg
      121 cgcaccggct ttttcagccg cgagtcctgg cgcctgccga agctgcctgc ctggccgtgg
      181 cagacgctgg cgaccggcgc gctcacggcg gctgcgctgg gcgccggcct gtggggtgcc
      241 ggactgctgc tcgaccggcc gatccgccag gtgatcgtgc aggggccgtt cgaacgcgtc
      301 agcgtgctgc agatcgaggc ggccgtcggc gagttgcgca acaccggttt cctgagcgtc
      361 cgcctcgacg ccgtgcgcgc caagatcgtc gccatcgact gggtcgacga tgccgtggtg
      421 cggcgacgct ggccggcgga ggtggagatc gtcgtccgcg agcaggtacc cgcggcgcgc
      481 tggggcgaca gcggcctgtt gaacacccgc ggtgagctgt tcgtccgtga cgcgcgccat
      541 atgccgccgg agctgccgca gctgtccggg ccggagggca ccgagcagct ggtggcgcgc
      601 cgctacctgg acgcccgcgc cacgctggcc gcggtcggcc tccgcctgcg tgcactcgag
      661 ctcgacgcgc gcggttcgtg gcggatcgag ctcagcaacg gcctgcagct gcgcctcggg
      721 cgcgagagct tcgagcggcg gctgcggcgc ttcgcccggg tcgcggcgcc gctgctcggc
      781 ccgcgcgcgg gcgaggtcgc ctacgtggac atgaggtaca gccgcggctt tgcggtgggc
      841 tggcgcgagg ccgcccccgg caacccggcc atgaaggaag gacttacgca cgatggctaa
      901 gcgcaacgcc agggacacga tcgtcggcct cgatatcggc acctcgaagg tggtggcgat
      961 cgtcggtgag gtcaccgagg agggcgggct ggaagtcatc ggcatcggca gccatccctc
     1021 gcgcggcctg aagaagggcg tggtggtgaa catcgagtcg acggtgctgt cgatccagcg
     1081 cgccgtcgag gaggccgagc tgatggccgg ctgcgagatc agcgacgtct acgccggcat
     1141 cgccggcagc cacgtacgca gcctgaactc gcacggcatc gtcgccatcc gcgagcgcga
     1201 agtgacgccg ggagacgtcg agcgcgtcat cgacgcggcg cgcgcggtgg ccattcccgc
     1261 cgaccagaag atcctgcaca tcattccgca ggagttcctg atcgaccacc aggaaggcat
     1321 ccgcgagccg atcggcatgt ccggcgtgcg cctcgaggcc aaggtgcaca tggtgaccgg
     1381 cgccgagagc gccgcgcaga acatcatcaa gtgcgtgcag cgctgcggac tgaacgtggt
     1441 cgacatcgtg ctcgagcagc tggcgtccag ccacgcggtg ctgtccgagg acgagaagga
     1501 gctcggcgtc tgcctggtgg acatcggcgg cggcaccacc gacatcgcgg tgttctccgg
     1561 cggcgcgatc cgccacacgg cggtcatccc gatcgccggc gaccaggtca ccaacgacat
     1621 cgcggtgtcg atgcgcacgc ccacgcagta cgcggaggag atcaagatcc gctacgcctg
     1681 cgcgctgtcg cagctggcga atcccgacga gaccatcgag gtgccgagcg tgggcgaccg
     1741 cccgccgcgg cggctggcgc ggcagacgct cgccgagatc gtcgagccgc gctacgagga
     1801 gctgttcacg ctgatccgca acgaggtggc gcgcagcggc ttcgacgaca tgatcgcggc
     1861 cggcgtggtg ctcacgggcg gcagcgcaaa gatggaaggc gcgatcgagc tggccgagga
     1921 ggtgttccac ctgccggtac ggctcggcgt gccgcacggc gtcaccggcc tggccgacgt
     1981 cgtgcgcaac ccgatccacg ccaccggggt cgggctcctg cagtacgcgc tgcacgagat
     2041 gcagcaacgg cagggcgagt caccgatgag cggcggcatc aaggacgtct gggcccgcat
     2101 gaaggcgtgg ttccagggaa atttctagtc acaaggggca ggggcaacaa tcccgaggag
     2161 agcggcaatg tttgaactgg tagacggata caacaccaac gcggtgatca aggtcatcgg
     2221 cgtgggcggc ggcggcggca acgccgtgtc ttacatgatg gagtgcggca tcgacggcgt
     2281 ggagttcatc tgcgccaaca ccgactcgca ggcgctgaag aactcgcgcg tcaagacggc
     2341 gatccagatc ggctgcaaca tcaccaaggg cctgggcgcc ggcgcgaacc cggacctcgg
     2401 ccggcaggcg gcgatggagg atcgcgatcg catccaggaa tcgatcgagg gtgcggacat
     2461 gctgttcatc accgccggca tgggcggcgg caccggcacc ggtgcggcgc cgatcgtggc
     2521 acaggtcgcc aaggagctcg gcatcctgac ggtcgcggtg gtgaccaagc ccttcggcat
     2581 ggagggtcgc aagcggctcg acatcgccga gatgggcatc accgagctct cgaagtacgt
     2641 cgactcgctg atcaccatcc ccaaccagaa gctgctgacg gtgctcggca agcagaccac
     2701 gctgctggac gccttcaagt cggccaacca ggtgctgcag ggcgcggtgc agggcatcgc
     2761 cgagctgatc acccggcccg gcctgatcaa cgtcgacttc gccgacgtcc gcacggtgat
     2821 ggccgagatg ggcatggcga tgatgggcac cggccgcggc accggcgagg accgggcgcg
     2881 cgaggcggcc gaggcggcca tctccagtcc gctgctggag gacatcaacc tcgagggcgc
     2941 caagggcatc ctggtcaacg tcactgccgg tctcgacctg gcgatcggcg agttcgacga
     3001 ggtcggcaac gtcgtcaagg agcgcgccca cgaggacgcc acggtagtgg tcggcacggt
     3061 catcgacccg gccatggaag gcgaggtgcg ggtcacggtg gtggccaccg gtctcggcgg
     3121 ggtgctgcgc cagcgcgagc cggacctgcg ggtggcctcc agcaacaccc gccagagccg
     3181 cgtgcgccgc agcgccggca tcgggccggt caactacagc gacctggagt cgccgccggt
     3241 ggtacgccgc cgcgccgacg aaccggagga catggtcgag gcgccgaagc gcgcggtcgg
     3301 ccagagcttc gagtaccgct ccgattccga gctgctcgac attccggcct tcctgcgtcg
     3361 ccaggccgac tgagcgccgg gccttgcgcc cggcgagcgc ttccggggga gccccgctct
     3421 ccttcccccg gaggcgctcg cccgggcgcc gggatgacga ttatgttacc ctgcgcagtt
     3481 agtttgtccg cgcggcgcgg gcagcatgga gccagtcgat gctgagacag cggaccttga
     3541 agaacaccat ccgggcgacc ggcgtgggcc tgcacaccgg cgagaaagtg ctgatgacgc
     3601 tgggcccggc ccccgtcaac accggcatcg tgttccggcg caccgacctc gatccgccgg
     3661 tggagatccg cgccgacggc atgcatgtcg gcgaaaccac cctcggcacc accctggtcc
     3721 gggacggcgt ccgcgttgcc accgtcgagc acctgatgtc ggccctggcg gggctcggca
     3781 tcgacaacgc gatcgtcgag ctgtcggcgc agga
//
LOCUS       DAOMPG010000231         3814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9924_length_3814_cov_3.556797, whole genome shotgun sequence.
ACCESSION   DAOMPG010000231 DAOMPG010000000
VERSION     DAOMPG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_3814_cov_3.556797"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLS34_10900"
     CDS             complement(<1..718)
                     /locus_tag="PLS34_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRP88009.1"
                     /translation="MNAAGPDREWLKRWLDHLRYERRLSALTVKNYHRDIEALYTFLC
                     NAEVADWAVVDGGHVRSFAARLHRRGQAPRSIQRRLAALRNLFDYLLREGVVKANPGV
                     DVTAPKAPRRLPDTLDADAMGRLLDVRVDHPIATRDRAIMELFYSSGLRLAELAGLQA
                     TDVDLLEATVRVLGKGAKTRIVPVGEKAREALRDWLKVRPQMVKDDATAMFISRNGRP
                     LSNRNIQARVAYWARRLGLPQ"
     gene            complement(720..1460)
                     /locus_tag="PLS34_10905"
     CDS             complement(720..1460)
                     /locus_tag="PLS34_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRP88010.1"
                     /translation="MTIRDERGLAATAPADQEIVDYLSAHPDFFERHAGLLEQLRLPH
                     RAGTATVSLVERQVSVLQDKNRALERRLHELVDVARSNTALSDKVHRLAIRLLQGGDT
                     AAVLAGLETGLREDFGASQAVVVLLHNTSEIVQGPRTRFLRAADRSDPALQPFATFMQ
                     QGKPRCGRIRDVQREFLFPGEETGIASVAMVPLGPKCEIGILAIGSSDGHRFNPTMST
                     DFLSRIGELAAAALAAARLRQEAARSGA"
     gene            complement(1457..2332)
                     /gene="dapF"
                     /locus_tag="PLS34_10910"
     CDS             complement(1457..2332)
                     /gene="dapF"
                     /locus_tag="PLS34_10910"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRP88011.1"
                     /translation="MRQTSAPAAATSLAFHKMVGAGNDFVVIDARARPFAPDAALAQR
                     LADRREGIGCDQLLLIEPDPSGAAAFAYRIWNADGGEVAQCGNGARCLALLWATELAP
                     GGPASFRMRSAAGLVEARVAAGAAAVCMGVPEFAPARIPLAATSEASRYTMELGDRSV
                     RLGAVSMGNPHAVIVLDEIGEQDIAHAPVATLGPAIERHAAFPQRVNVGFARPLGHDR
                     IALRVWERGVGETLACGTGACAAVAVLHDGGRVDPVVTVELPGGELVVEWHGRGTPAW
                     LSGPSRKVFEGTISL"
     gene            2380..>3814
                     /gene="ubiD"
                     /locus_tag="PLS34_10915"
     CDS             2380..>3814
                     /gene="ubiD"
                     /locus_tag="PLS34_10915"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HRP88012.1"
                     /translation="MKYGDLRDFLRLLEAQGELKRIGAPVSPVLEMTEICDRVLKQGG
                     PALLFENPVGHDMPVLGNLFGTPRRVALGMGQDSVEALREVGKLLASLKEPDPPRGMR
                     DAWDKLPIFRQVLNMAPKVVRSAPCQQNFVAGPDVDLEKLPIQTCWPGDAGPLRTWGL
                     VITRGPERARQNLGIYRQQLIGRNRLIMRWLSHRGGALDFRDFQLRHPGEPFPVAVAL
                     GADPATILGAVTPVPDTLSEYAFAGVLRGGRTEVVKCALSDLEVPAGAEIVLEGHLAP
                     GDNAPEGPFGDHTGYYNEVDEFPVFTVDRISWRDDPIYHSTYTGRPPDEPAVLGVALN
                     EVFVPILQKQFPEIVDFYLPPEGCSYRMAVVSMKKQYPGHAKRVMLGIWSFLRQFMYT
                     KFVIVTDDDVDVRNWQDVIWAMTTRMDPARDTVMIENTPIDYLDFASPVSGLGSKIGF
                     DATNKWKGETDREWGRPISMDPEVKARI"
BASE COUNT          544 a         1354 c         1371 g          545 t
ORIGIN      
        1 cctgcggcag ccccaggcgg cgtgcccagt aggcgacgcg cgcctggata ttgcggttcg
       61 acagcggccg gccgttgcgg ctgatgaaca tggcggtggc gtcgtccttg accatctgcg
      121 gccgcacttt cagccagtcg cgcagcgcct cgcgcgcctt ctcgcccacc ggcacgatgc
      181 gcgtcttggc gcccttgccg agcacccgca cggtggcctc cagcaggtcg acgtcggtcg
      241 cctgcagccc ggccagctcc gccagccgca gccccgagga atagaacagc tccatgatgg
      301 cgcggtcgcg ggtggcgatc gggtggtcca cacgcacgtc cagcaggcgg cccatggcgt
      361 ccgcgtccag cgtgtcgggc agccggcgcg gcgccttcgg ggcggtgacg tccacgcccg
      421 ggttggcctt caccacgccc tcgcgcagca ggtagtcgaa caggttgcgc agcgccgcca
      481 ggcgccgctg gatgctgcgc ggggcctggc cgcggcggtg cagccgggcg gcgaaggagc
      541 gcacgtggcc gccgtccacc accgcccagt cggcgacctc ggcattgcac aggaaggtgt
      601 acagcgcctc gatgtcgcgg tggtagttct tcacggtcag tgccgacagg cggcgctcgt
      661 agcgcaggtg gtcgagccag cgcttgagcc attcgcggtc ggggccggcc gcgttcatgt
      721 catgcgccgc tgcgcgcggc ctcctgcctg agccgcgcgg cggccagcgc cgcggcggcc
      781 agctcgccga tgcgcgacag gaaatccgtg ctcatcgtcg ggttgaagcg gtgcccgtcg
      841 ctgctgccga tggccaggat gccgatctcg cacttcgggc cgagcggcac catcgccacc
      901 gaggcgatgc cggtctcctc gcccgggaac aggaactcgc gctgcacgtc gcggatgcgt
      961 ccgcagcgcg gcttgccctg ctgcatgaag gtggcgaagg gctgcagcgc cgggtcggag
     1021 cggtccgcgg cgcgcaaaaa gcgggtccgc gggccctgca cgatctccga ggtgttgtgc
     1081 agcagcacca ccaccgcctg gctggcgccg aagtcctcgc gcaggccggt ctccaggccc
     1141 gccagcacgg cggccgtgtc gccgccctgc aagagccgga tggccagccg gtggaccttg
     1201 tcggacagcg cggtgttcga gcgggccacg tccaccagtt cgtgcagccg ccgctccagg
     1261 gcgcggttct tgtcctgcag cacgctcacc tggcgctcca ccagcgacac cgtggcggtg
     1321 ccggcgcggt gcggcaggcg cagctgctcg agcagcccgg cgtggcgctc gaagaaatcc
     1381 ggatgcgcgg acaggtagtc cacgatctcc tggtcggcgg gcgccgtggc ggcaaggccg
     1441 cgttcgtctc ggatggtcac aggctgatgg tcccctcgaa gactttcctg gatgggccgc
     1501 tgagccaggc cggcgtgccg cggccgtgcc actcgacgac cagctcgccg cccggcaact
     1561 ccaccgtgac gaccgggtcc acccggccgc cgtcatgcag caccgccacg gccgcgcagg
     1621 caccggtgcc gcaggccagc gtctcgccca cgccgcgctc ccagacgcgc agcgcgatgc
     1681 ggtcgtggcc cagcggccgg gcaaagccga cgttcacgcg ctgcgggaac gccgcgtggc
     1741 gctcgatggc cgggcccagc gtcgcgaccg gcgcgtgggc gatatcctgt tcgccgattt
     1801 cgtccagcac gatcaccgca tgcgggttgc ccatggacac ggcgccgagc cggacgctcc
     1861 ggtcgccgag ttccatggta tagcgcgagg cctcgcttgt cgcggccagc gggatgcgcg
     1921 ccggcgcgaa ctccggtacg cccatgcaca ccgccgccgc gccggcggcg acgcgggcct
     1981 ccaccagccc ggccgcgctg cgcatgcgga agctcgccgg cccgcccggc gccagctccg
     2041 tggcccacag cagcgccagg cagcgggcgc cgttgccgca ctgcgcgacc tcgccgccgt
     2101 cggcgttcca gatgcggtag gcgaaggccg cggccccgct cgggtccggc tcgatcagca
     2161 gcagctgatc gcagccgatg ccttcgcgcc ggtcggccag ccgctgtgcc agcgcggcgt
     2221 cgggtgcgaa cggccgggcg cgcgcgtcga tcaccacgaa gtcgttgccg gcgcccacca
     2281 tcttgtgaaa ggcgagactg gtggcggcag cgggggcgga agtttggcgc atggctgggg
     2341 cgcaattata gtgggcgcgc cgcagtcatg gtggcgggaa tgaaatacgg cgatctcagg
     2401 gattttctgc gcctgctcga ggcgcagggc gagttgaagc gcatcggcgc gccggtcagc
     2461 ccggtgctcg agatgacgga gatctgcgac cgcgtgctga agcagggcgg tcccgccctc
     2521 ttgttcgaaa acccggtcgg ccacgacatg ccggtgctgg gcaacctgtt cggcacgccg
     2581 cggcgggtcg cgctcggcat gggccaggac tccgtcgagg cgctgcgcga ggtcggcaag
     2641 ctgctggcct ccctgaagga gccggacccg cccaggggca tgcgcgacgc ctgggacaag
     2701 ctgccgatct tccgccaggt gctcaacatg gcgcccaagg tggtgcgctc cgcgccctgc
     2761 cagcagaact tcgtcgcggg cccggacgtg gacctcgaga agctgccgat ccagacctgc
     2821 tggccgggcg acgccggccc gctccggacc tgggggctgg tgatcacccg cggcccggag
     2881 cgcgcccgcc agaacctcgg catctaccgc cagcagctga tcggcaggaa ccgcctgatc
     2941 atgcgctggc tgtcgcaccg cggcggcgcg ctggatttcc gcgatttcca gctcaggcac
     3001 ccgggcgagc cgttcccggt cgccgtggcc ctgggcgccg accccgcgac catcctcggc
     3061 gcggtcacgc cggtgccgga cacgttgtcg gaatacgcgt tcgccggggt gctgcgcggc
     3121 ggccgcaccg aggtagtgaa gtgcgcgctg tcggacctcg aggtgccggc cggcgcggag
     3181 atcgtgctgg agggccatct cgcgccgggc gacaacgcgc ccgaggggcc gttcggcgac
     3241 cacaccggtt actacaacga ggtggacgag ttcccggtgt tcaccgtgga tcgcatcagc
     3301 tggcgcgacg acccgattta ccacagcacc tacaccggcc ggccgccgga cgagccggcc
     3361 gtgctcggcg tggcgctcaa cgaggtgttc gtgcccatcc tgcagaagca gttcccggag
     3421 atcgtggatt tctacctgcc gccggagggc tgttcctacc ggatggcggt ggtcagcatg
     3481 aagaagcagt acccgggcca cgccaagcgc gtcatgctgg gcatctggtc cttcctgcgg
     3541 cagttcatgt acaccaagtt cgtgatcgtc accgatgacg acgtggacgt gcgcaactgg
     3601 caggacgtca tctgggcgat gaccacgcgc atggacccgg cgcgcgacac ggtgatgatc
     3661 gagaacacgc ccatcgatta cctcgatttc gcctcgcccg tgtcggggct cggctcgaag
     3721 atcggcttcg acgccaccaa caaatggaaa ggcgagacgg accgggaatg ggggcggccg
     3781 atcagcatgg acccggaagt gaaggcgcgc atcg
//
LOCUS       DAOMPG010000232         3802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_9961_length_3802_cov_3.268481, whole genome shotgun sequence.
ACCESSION   DAOMPG010000232 DAOMPG010000000
VERSION     DAOMPG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9961_length_3802_cov_3.268481"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLS34_10920"
     CDS             <1..942
                     /locus_tag="PLS34_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP88013.1"
                     /translation="MTAPGGGGGGGGGGGGGGGGGGGGGGGAATPVPIDGDESLKVYS
                     LAAEYEYTPGGRFSAVAGLSQNFQSADTAGSESDTGFVLGGRYRLGEYSALRAMVSRQ
                     IRFPTLRDLFAADRGNPELEPERTMNYEIGFEHLLADGAATLELAVFRIDARDFIERP
                     PGGGVSENFDEYRFQGVEFTAAYRPTDALTLAFGYTWLDSENRSPGADTTILQNRPEH
                     KYSLTGDYDFASGTRLRLELLRATDSYALSRSTPTTALKLDSYTVANVVVSQSFRNDA
                     ISVFARAQNLLDEDYAESFGYQQPGRTLYLGVELRTR"
     gene            complement(955..1767)
                     /locus_tag="PLS34_10925"
     CDS             complement(955..1767)
                     /locus_tag="PLS34_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP88014.1"
                     /translation="MKIRHVVALGCILAGLAAPLQAQDRFIVVASTTSTENSGLFGHL
                     LPLFRDATGIEVRVVAVGTGQAFAIARRGDADGLLVHDTAGEELMVSDGFALERRNVM
                     YNDYVIVGPAADPAGIRDAASAAEAFTRIAGRTTPFVSRGDDSGTHRAELRIWRQAGL
                     SPHGSWYREVGSGMGATLNTAAGMSAYVLADRGTWISFANRQDLEVLFEGDPELFNQY
                     GSVLLDPARNAHLKHDLARQWHDWLLSEAGQAAIASFQLDGQPLFFPNAGRP"
     gene            1951..2469
                     /gene="moaB"
                     /locus_tag="PLS34_10930"
     CDS             1951..2469
                     /gene="moaB"
                     /locus_tag="PLS34_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HRP88015.1"
                     /translation="MTERSFVPLNIAVLTVSDSRGEAEDKSGKLLVERLEKAGHRCAE
                     KRIVRDDVYAIRAVVSAWIADLEVQAVITTGGTGVTGRDGTPEAVAPLLDKTLDGFGE
                     IFRMISWQQIKTSTIQSRAIAGVANGTYIFCLPGSSGACATGWDEIIEAQLDRRTRPC
                     NLVELMPRLLET"
     gene            2570..3535
                     /locus_tag="PLS34_10935"
     CDS             2570..3535
                     /locus_tag="PLS34_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88016.1"
                     /translation="MLHTCAPPDEPGVIYAAAGHKIWGAHIYRSEDGGRSWTSLPAAP
                     QHAPGAHRTALKAIWYLAAAPGGRLYAGIDPAGLFVSTDRAASWQPVTALNEHPTRDT
                     WEPARGGFSVHSIRIDPADPARLWVAVSAGGVYRSEDAGASWTPANAGVRAENLPTPY
                     PESGHNVHRLVMHPARADRLYRQCYHGAWRSDDAGRSWTEITAGLPGNFGYAIAADPR
                     DPDTVYQVPESDSHMRTVVDGKLRVYRSHDAGASWSSASDGLPQAHAWVTVLREAMDT
                     DDSGGVYFGTSGGHLFASRDGAESWQLAAGFLPRILSVKAVRAAP"
     gene            3532..>3802
                     /locus_tag="PLS34_10940"
     CDS             3532..>3802
                     /locus_tag="PLS34_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HRP88017.1"
                     /translation="MSELLDLLHARDGIVCATGAGGKKSVLFAIAARHPGRVAFTTTV
                     HTLPPPEALGAAVIVAPEQQLQAELHGHADARFIAYACPGDKPGRL"
BASE COUNT          590 a         1323 c         1327 g          562 t
ORIGIN      
        1 atgaccgcgc ccggcggtgg cggtggcggt gggggtggcg gtggcggtgg cggcggcggc
       61 ggtggcggtg gcggtggcgg cgccgcgacg ccggtgccga tcgacggcga cgagtcgctc
      121 aaggtctact cgctggccgc ggaatacgag tacacgcccg gcggccggtt ctcggccgtc
      181 gccgggctga gccagaactt ccagagcgcc gacacggccg ggtccgaaag tgacacgggc
      241 ttcgtgcttg gggggcgcta ccgcctcggc gagtactcgg cgctgcgcgc gatggtttcg
      301 cgccagatcc gtttcccgac cctgcgcgac ctgtttgccg ccgaccgggg caacccggag
      361 ctggagcctg agcgcaccat gaattacgag atcggcttcg agcacctgct ggcggacggc
      421 gccgcgacgc tggagctggc ggtgttccgc atcgacgcgc gcgacttcat cgagcgtccg
      481 cccggtggcg gcgtgtccga aaatttcgac gaatatcgct tccagggcgt ggaattcacg
      541 gcggcctatc gtcccaccga tgccctcaca ctggcctttg gctacacctg gctcgattct
      601 gagaatcgct cgccgggcgc tgacacgacg atcctgcaga accggccgga gcacaagtat
      661 tccctgacgg gcgactacga cttcgcgtcg ggcacacgcc tgcgactgga actgctgcgg
      721 gcgacggaca gctatgcgct gtcgcgcagc acgccgacca cggcgctcaa gctggacagc
      781 tacaccgtgg ccaacgtcgt ggtgtcacag tcgttcagga acgatgcgat cagcgtgttt
      841 gcccgggccc agaacctgct ggacgaagat tacgccgagt ccttcggcta ccagcagcct
      901 ggcaggacgc tgtatctcgg cgtggagctg cgaacgcgct gaccggcctt tgactcacgg
      961 cctgccggca ttgggaaaga acagcggctg gccgtcgagc tggaacgagg cgatggcggc
     1021 ctgccccgcc tcggacagca gccagtcgtg ccactggcgc gccaggtcat gcttgagatg
     1081 tgcattgcgg gccgggtcga gcaacacgct gccgtactgg ttgaacagct ccgggtcgcc
     1141 ttcgaacagc acctccaggt cctggcggtt tgcgaagctt atccaggtgc cgcggtcggc
     1201 cagcacatag gcgctcatgc cggccgccgt gttcagggtg gcgcccatgc cgctgcccac
     1261 ttcgcggtac cagctgccgt gggggctgag gccggcctgc cgccagattc gcagctcggc
     1321 ccggtgcgtg ccgctgtcgt cgcctcgcga cacgaagggc gtcgtacgcc cggcaatgcg
     1381 ggtgaaggcc tcggcagcgg atgcggcgtc gcggatgccg gcaggatcgg ccgcggggcc
     1441 gacgatgacg tagtcgttgt acatgacgtt gcgccgttcg agagcgaagc cgtcgctgac
     1501 catcagttcc tcgccggctg tgtcatggac cagcagtccg tcggcatcgc cccggcgtgc
     1561 gatggcaaag gcctggccgg tgcccaccgc cacgaccctg acctcgatgc ccgtggcgtc
     1621 acgaaacaac ggcaggaggt ggccgaacag accggaattc tcggtcgaag tcgtggacgc
     1681 cacgacgatg aagcgatcct gggcctgcaa gggcgccgcc aggccggcca ggatgcaacc
     1741 taatgcgacg acgtgacgaa ttttcatgaa ccatcctcca gctcggcaag ccaccgtccg
     1801 ttcgaccgac attatccgtg atttccatcg cgggcgaacc ctgcacggcc cccggacggc
     1861 gcggcgacga gcggcccgcc gcctggacgt ctcccccggc ggagccatgg cttacaatac
     1921 ggaaccgcat ttcacgcccg gaaccgcgcc atgaccgaac gcagcttcgt ccccctcaac
     1981 atcgccgtcc tgacggtctc agacagccgc ggcgaggccg aggacaagtc gggcaagctg
     2041 ctggtcgagc ggctcgagaa agcaggtcat cgctgcgccg agaagcgcat cgtgcgcgac
     2101 gacgtgtatg ccatccgggc ggtggtgtcg gcgtggatcg ccgaccttga agtgcaggcg
     2161 gtgatcacca ccggcggcac cggcgtgacc ggccgtgacg gcacgcccga ggcggtggcg
     2221 ccgctgctcg acaagacgct cgacggtttc ggggagattt tccgcatgat ctcctggcag
     2281 cagatcaaga cctcgaccat ccagtcgcgc gccatcgccg gcgtcgccaa cggcacctac
     2341 atcttctgcc tgccgggctc ctcgggcgcc tgcgccaccg gctgggacga gatcatcgag
     2401 gcgcagctcg atcggcgcac gcgaccctgc aacctggtgg agctgatgcc gaggttgctg
     2461 gagacgtgag cggcggcgac ccggccctgc tggtcggcac gcagaagggc ctgttcatcg
     2521 tgacgcccgc cgctggcggc tggacggccc gcacatcgcg ggctacgagg tgctccacac
     2581 ctgcgccccg cccgatgagc ccggcgtcat ctacgcggcg gccggccaca agatctgggg
     2641 tgcgcacata tacaggagcg aggacggtgg tcgctcgtgg acttcgttgc cggcggcgcc
     2701 gcagcacgcg cccggcgcgc atcgcaccgc cctgaaggcg atctggtacc tggccgcggc
     2761 gccgggcggg cggctctacg ccggcatcga cccggcgggg ctgttcgtca gcacggaccg
     2821 cgccgcaagc tggcagccgg tgacggcgct caacgagcac ccgacccgcg acacctggga
     2881 accggcgcgc ggcggctttt cggtccactc catccgcatc gatccggccg acccggcgcg
     2941 cctctgggtg gcggtgtccg ccgggggcgt gtaccgcagc gaggacgccg gtgcgagctg
     3001 gacgcccgcc aatgccgggg tcagagccga gaatctgccg acgccgtacc cggagagcgg
     3061 ccacaacgtg catcgcctgg tcatgcaccc ggcccgcgcg gaccgcctgt accggcagtg
     3121 ctaccacggc gcgtggcgca gcgacgacgc cggacgcagc tggacggaga tcaccgcagg
     3181 gctgcccggc aacttcggct acgccatcgc ggcggacccg cgtgacccgg acacggtgta
     3241 ccaggtgccg gaatcggact cgcacatgcg cacggtggtg gacggcaagc tgcgcgtcta
     3301 ccgcagccac gacgccggcg cgagctggtc gtccgccagc gacggcctgc cgcaggcgca
     3361 cgcctgggtg acggtgctgc gcgaggcaat ggatacggac gactcgggcg gcgtgtattt
     3421 cggcacctcc ggcggacacc tgttcgccag ccgcgacggc gccgagtcct ggcagctcgc
     3481 ggccggcttc ctgccgcgca tcctgtcggt gaaggccgtg cgagcggcgc cgtgagcgag
     3541 ctgctcgacc ttttgcacgc ccgcgacggg atcgtctgcg cgaccggtgc aggcggcaag
     3601 aagagcgtgc tgttcgccat tgccgcccgc cacccgggac gcgtcgcttt caccactacc
     3661 gtgcacacct tgccgccgcc cgaggcgctc ggcgccgcgg tgatcgtcgc gccggagcag
     3721 caactccagg ccgagctgca cggccatgcc gacgcccggt tcatcgccta tgcctgcccg
     3781 ggcgacaagc cgggccgcct ca
//
LOCUS       DAOMPG010000233         3759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10084_length_3759_cov_3.232991, whole genome shotgun sequence.
ACCESSION   DAOMPG010000233 DAOMPG010000000
VERSION     DAOMPG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_3759_cov_3.232991"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLS34_10945"
     CDS             <1..380
                     /locus_tag="PLS34_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HRP88018.1"
                     /translation="PRAPLPVSVPVHCQLMDPVSERLAAALEDIAVRVPAIPVIHNVD
                     AAPRRDPAGIRAALAEQVCRPVRWVECVRALQGAGAGTLVEIGPGRVLCGLARRIDRG
                     LAAYPVDDPDTLAAALAAAEGAA"
     gene            377..1123
                     /gene="fabG"
                     /locus_tag="PLS34_10950"
     CDS             377..1123
                     /gene="fabG"
                     /locus_tag="PLS34_10950"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRP88019.1"
                     /translation="MSQLQDEVALVTGASRGIGRAIADALGRAGATVVGTATSPAGAE
                     AVGAAFAAAGIKGRGIVLDVASDADVDAAIADIAAREGAVTLLVNNAGITRDGLLLRM
                     KIEDWQQVIDTDLTSLYRCSKACLRGMMKARRGRIINIGSVVGLMGNPGQANYCAAKA
                     GLVGFTRALAREVGSRGITVNAVAPGFIETDMTAALSAAQRDSLVQQIPLERLGAAED
                     IAATVLFLCSPGAGYITGEVINVNGGMYMA"
     gene            1245..1481
                     /gene="acpP"
                     /locus_tag="PLS34_10955"
     CDS             1245..1481
                     /gene="acpP"
                     /locus_tag="PLS34_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRP88020.1"
                     /translation="MSSIEEQVKNIVAEQLGVKEEEVTNDASFVDDLGADSLDTVELV
                     MALEEAFECEIPDEEAEKITTVQQAIDYIKARSN"
     gene            1555..2802
                     /gene="fabF"
                     /locus_tag="PLS34_10960"
     CDS             1555..2802
                     /gene="fabF"
                     /locus_tag="PLS34_10960"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRP88021.1"
                     /translation="MTRRRVVVTGLGLVSPVGSTAASAWANIVAGRSGIAPITRFDVS
                     RFSTRFGGAVADFDVEQYLSSKDARKMDAFMHYGFAASRQAVEDAGIEVTPQNAHRMG
                     VAVGAGIGGIETIERSFRAYLDAEESPRKISPFFIPGSIINMISGHVSIHYGMTGPNL
                     SLVTACTTATHCIGIAGRTIQYGDADVMVAGGAEMATTALGLGGFCSARALSQRNDEP
                     ARASRPWDRDRDGFVLGDGAGCVVLEEYEHARARGASIYGELAGFGMSADAHHITAPP
                     EDGEGARQCMLNALRDAGVNPDEVDYINAHGTSTPLGDKGETFAIKRAFGDHARKVAV
                     SSTKSMTGHLLGAAGGVEAVFSLLAIRDGVLPPTINYETPDPDCDLDYVPNEARRAKV
                     RLALSNSFGFGGTNGSLLFRALD"
     gene            2852..>3759
                     /locus_tag="PLS34_10965"
     CDS             2852..>3759
                     /locus_tag="PLS34_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRP88022.1"
                     /translation="MTPVCSEIDDPGDLLPLHRLAPARYPFLLESAASGTAQGRCDLL
                     LGFPGEALVLARDGRLHGPGAGDDHDFLAALDRWWRRERHARPDAWPLAGGGWFLYLG
                     YELAGQVEPVLRLDAPRALPIAFAVRAGAAVLRDRANGCCRVMARDAETLRRVESDLA
                     AAARLEPTHWPGPADNAVEEPPERYLQAVRRAREYIIAGDVFQANLSREWRAPLAPDA
                     DPAALYARLRRSNPGPFAGLARWGDHAVLSSSPERLLSVREGVVQTRPIAGTRPRGAN
                     AADDLALERELIAHPKERAEHVMLIDL"
BASE COUNT          547 a         1314 c         1363 g          535 t
ORIGIN      
        1 cgccgcgggc gccgctgccg gtgagcgtgc cggtgcattg ccagctcatg gacccggtga
       61 gcgagcgact ggcggccgcg ctggaggaca tcgcggtgcg agtgccggcg atcccggtca
      121 ttcacaacgt ggacgcggcg ccgcggcggg atcccgccgg cattcgcgcg gcgctggcgg
      181 agcaggtgtg ccgcccggtg cgctgggtcg aatgcgtgcg cgccctgcaa ggcgccggcg
      241 ccggtacgct ggtggaaata ggtccgggcc gcgtgctgtg cggcctcgcc aggcggatcg
      301 accgcgggct ggccgcctat cccgtagacg atcctgatac ccttgcggct gcactcgcag
      361 cagcggaggg ggcggcatga gccagttgca ggacgaggtg gcgctggtca ccggggcatc
      421 gcgcggcatc gggcgcgcca tcgcggatgc gctgggccgg gccggcgcca cggtcgtcgg
      481 cacggcgact tcgccggccg gggccgaggc cgtcggcgcc gcctttgcgg ccgccggcat
      541 caagggccgc ggaatcgtgc tggacgtggc cagcgatgcg gacgtcgacg ccgccatcgc
      601 cgacatcgcc gctcgcgagg gggccgtgac gctgctggtg aacaatgccg gcatcactcg
      661 cgacgggctt ctgctgcgca tgaagatcga ggactggcag caggtcatcg acaccgacct
      721 gacgtcgctc taccgctgca gcaaggcctg cctgcggggc atgatgaagg cccgccgggg
      781 ccgcatcatc aacatcggct cggtggtggg cctgatgggc aaccccggcc aggccaacta
      841 ttgcgccgcc aaggccgggc tggtcggctt cacgcgggcg ctggcgcgcg aggtcggctc
      901 gcgcggcatc accgtcaacg cggtggcgcc gggcttcatc gagaccgaca tgaccgcagc
      961 cctgtcggcc gcgcagcgcg actcgctggt gcagcagatc ccgctcgaac ggctgggcgc
     1021 ggccgaggac atcgccgcca cggtgctgtt tctttgttca cccggggccg gttacatcac
     1081 cggcgaggtc atcaatgtca acggggggat gtacatggcc tgagggcccc ggggcgcctc
     1141 attgtagaac cccctgtaac attccaatac aatacggcgc gcggtgcagt ccgggtcatg
     1201 gggatcggga tgatctcgcc gccagtaacg aggatgagga cgagatgagc agcatcgaag
     1261 aacaggtaaa gaacatcgtc gccgagcagc tgggcgtcaa agaagaagaa gtcaccaacg
     1321 acgcctcgtt cgtggacgac ctgggcgcgg actcgctgga taccgtcgaa ctcgtgatgg
     1381 cgctcgaaga ggccttcgag tgcgagatcc cggacgagga agccgagaag atcacgaccg
     1441 tccagcaggc catcgactac atcaaggccc gctcgaactg aagcggccgt ccgggcgccc
     1501 gcttccaggg cgccgctttc ccggccgcca ggcagatccg ccggaggtga acgcttgacc
     1561 aggcgccgtg tcgtcgtcac cggcctcggc ctcgtgtcgc ccgtgggcag cacggccgct
     1621 tccgcgtggg cgaacatcgt cgccggccgc agcggcatcg cgccgatcac gcgcttcgac
     1681 gtcagccggt tttccacgcg cttcggcggg gccgttgcgg acttcgacgt cgagcagtac
     1741 ctgtcgtcga aggacgcgcg caagatggac gccttcatgc actacggctt tgccgcctcg
     1801 cgccaggccg tggaggatgc gggcatcgag gtgacgccgc agaacgcaca ccggatgggc
     1861 gtcgcggtcg gcgccggcat cggcggcatc gagaccatcg agcgctcctt ccgcgcctac
     1921 ctcgatgcgg aagaatcgcc gcgcaagatt tcgccgttct tcatccccgg cagcatcatc
     1981 aacatgatct ccggccacgt ctcgatccac tacggcatga ccggaccgaa cctgtcgctg
     2041 gtcaccgcct gcacgaccgc cacccactgc atcggcatcg ccggacgcac catccagtac
     2101 ggcgacgcgg acgtgatggt ggccggcggc gcggaaatgg cgaccacggc gctcggcctc
     2161 gggggcttct gctcggcgcg cgcactgtcc cagcgcaacg acgaacccgc gcgcgccagt
     2221 cggccgtggg accgggatcg cgacggcttc gtgctcggcg acggcgcagg ctgcgtggtg
     2281 ctggaggagt acgagcacgc cagggcgcgc ggggcgagca tctacggcga gctggcgggc
     2341 ttcggcatga gcgcggacgc gcaccacatc acggcgccgc ccgaggacgg cgagggcgcg
     2401 cgccagtgca tgctcaacgc gctccgcgac gccggcgtca atcccgacga ggtggactac
     2461 atcaatgcgc acggcacctc gaccccgctg ggcgacaagg gcgagacgtt cgccatcaag
     2521 cgcgccttcg gcgatcacgc gcgcaaggtc gcggtcagtt ccaccaagtc catgaccggt
     2581 cacctgctcg gtgccgcggg cggcgtggag gcggtgttca gcctgctggc gattcgcgac
     2641 ggcgtgttgc cgccgaccat caactacgag acgcccgatc ccgactgcga cctggactat
     2701 gtgccgaacg aggcgcgccg ggcgaaggtg cgcctggcgc tctccaactc ctttggcttc
     2761 ggcggcacca acggcagcct gttgttccgg gcgctcgact agcgccgccg cacggcgcgg
     2821 cgtgccggtg gcggccctgt atccttcccg catgacgccc gtctgcagcg agatcgacga
     2881 tcccggcgac ctgctgccgc tgcaccgcct ggcgccggcg cgctatccct tcctgctgga
     2941 gagcgccgcc tccggcacgg cgcaggggcg ctgcgacctg ttgctggggt ttccgggcga
     3001 ggcgctggtg ctggcgcgcg acggccggct gcacgggccc ggcgcgggcg acgaccacga
     3061 cttcctcgcc gcactcgacc gctggtggcg gcgcgagcgc cacgcccgtc cggacgcctg
     3121 gccgctggcc gggggcggct ggttcctgta cctgggctac gagctggccg ggcaggtgga
     3181 gccggtgctg cgtctcgatg cgccgcgcgc gctgccgatc gcctttgccg tgcgcgccgg
     3241 ggcggccgtg ctgcgcgacc gcgccaatgg ctgctgccga gtgatggcgc gggacgccga
     3301 aaccctccgc cgcgtcgagt ctgacctggc cgcggcggcc cggctggagc ctacgcactg
     3361 gccgggaccg gccgacaacg cggtggagga gcctcccgag cgctacctgc aggccgtgcg
     3421 ccgcgcacgc gagtacatca tcgccggtga cgttttccag gccaacctgt cccgcgagtg
     3481 gcgcgccccg ctggccccgg atgcggaccc ggcggcgctc tacgcgcggc tgcggcgcag
     3541 caacccgggc ccgttcgccg ggctggcccg ctggggcgac catgccgtgc tcagttcgtc
     3601 gccggagcgt ctgctcagcg tgcgcgaggg cgtcgtgcag acgcggccga tcgcgggcac
     3661 gcggccgcgc ggcgcgaacg cggccgatga cctggcgctg gagcgcgagc tgattgcgca
     3721 tcccaaggaa cgcgccgagc acgtgatgct gatcgacct
//
LOCUS       DAOMPG010000234         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10172_length_3730_cov_2.940136, whole genome shotgun sequence.
ACCESSION   DAOMPG010000234 DAOMPG010000000
VERSION     DAOMPG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10172_length_3730_cov_2.940136"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="PLS34_10970"
     CDS             complement(<1..1896)
                     /locus_tag="PLS34_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HRP88023.1"
                     /translation="MKVDRKARTGRIGWREIARRGLALAALLLPGIVWFPADAAVDPW
                     AAGAGSLWLRADADAAPQAAPGMASAFHARVTGNTARVTLTQRFSNPTDDWMEGLYVF
                     PLAATAAVDQLELEVGDRVIHGAIQPREIARKTYETARAAGRRAGLVDQARPNMFTTA
                     VANIPPRGEITVRIAYLEIIPWRDGHYTLRLPLAITPRYTPGAEPGAAGAIELRIAAA
                     EGELPARCATPELVSADSGHAHIEIELAAGFPLASLTSHHHPVSSEESGGQVRILAAR
                     AAADRDFELTWAPVIAPDTHAAVYTEQFDGETHALVLLTPPQAPSGAPPPREVIFVID
                     TSGSMGGPSIAQARAALALGVQRLGPHDRFNIIRFSNHAAALFAASRPVDPLARQVAN
                     RFIASLEASGGTEMRAAVELAFATPPADGVLRQVVFITDGSVGNEAEIVALIRRRLGD
                     ARLFTVGIGAAPNAWFMHEAAAAGRGSYTFIARPEQVRERMAGLFMKLERPALVGLEL
                     HWPTGVEADLATPLPRDLYAGEPLVVAARLQGAPLQGLLTLAGQDAGGHRLRQAPVVA
                     LEGEAGIARLWARERIAELSRRKRLGDDAASPAELDRRILALAMRYGLVSELTSLVAV
                     DNTPVRPP"
     gene            complement(2049..2540)
                     /locus_tag="PLS34_10975"
     CDS             complement(2049..2540)
                     /locus_tag="PLS34_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRP88024.1"
                     /translation="MSATDEFVAANRRYAAQFTKGDKPMPPAKRVAVVACMDARIETG
                     RLLGLEEGDAHVIRNAGGVVTDDVIRSLAISQRLLGTTEIVLIHHTDCGMVTFKDDAL
                     KAQIEADTGLRPPFALEAFADPADDIRQSVRRIKSSPFVPHRDKVRGFVYDVASGRLN
                     EVT"
     gene            complement(2628..3548)
                     /locus_tag="PLS34_10980"
     CDS             complement(2628..3548)
                     /locus_tag="PLS34_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRP88025.1"
                     /translation="MAGASLLALLDDIATVLDDVALLTKATAKKTAGVLGDDLALNAQ
                     QVSDVRADRELPVVWAVARGSFVNKAVLVPAALAISALAPWAVMPLLMAGGLFLCYEG
                     CEKLAHKFLHEKGERAAHAAELADALADPAIDLVALEKDKIRGAVRTDFILSAEIIVI
                     ALGIVASAPFGTRVMVLVTIAVLMTIGVYGLVAAIVKLDDFGLHLARRPGEGAVQVLG
                     RGIVAAAPYLMKGLAIAGTAAMFLVGGGILTHGVPGLESWLEQLGNGFGTGGGALLLL
                     LSNALVGVLAGAVVLAVVSGVRKWRHSAKA"
     gene            complement(3574..>3730)
                     /locus_tag="PLS34_10985"
     CDS             complement(3574..>3730)
                     /locus_tag="PLS34_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRP88026.1"
                     /translation="YGRVPAGAVVVPGSLPSSEGKCHLACAVIVKRVDAATRAKVGIN
                     ELLRAAR"
BASE COUNT          530 a         1307 c         1364 g          529 t
ORIGIN      
        1 gggcgggcgc accggcgtgt tgtccaccgc caccaggctg gtcagctcgc tgaccaggcc
       61 gtagcgcatc gccagggcga gaatgcgtcg gtcgagttcg gcgggcgatg cggcgtcgtc
      121 gcccaggcgc ttgcggcgcg acagctcggc gatgcgctcg cgcgcccaca gccgcgcgat
      181 gcccgcttca ccctcgagcg ccacgaccgg cgcctgccgc aggcgatggc cgccggcgtc
      241 ctggcccgcc agggtgagca ggccctgcag cggcgcgccc tgcaaccgcg ccgccaccac
      301 cagcggttcg cccgcataca ggtcgcgcgg cagcggcgtg gcgagatcgg cttcgacgcc
      361 cgtcggccag tgcagttcga gtccgaccag cgccggtcgc tcgagcttca tgaacagccc
      421 ggccatgcgc tcgcggacct gctcggggcg cgcgatgaag gtgtaactgc cgcgcccggc
      481 cgcggcggcc tcgtgcatga accaggcgtt cggcgcggcg ccgatgccca ccgtgaacag
      541 ccgcgcatcg ccgagccggc gccggatcag cgcgacgatc tctgcctcgt tgccgacgct
      601 gccgtcggtg atgaacacca cctggcgcag cacaccgtcg gccggcggcg tcgcgaaggc
      661 gagctcgacg gcggcgcgca tctcggtgcc gccgctcgcc tcgagcgaag cgatgaaacg
      721 gttcgccacc tggcgcgcga ggggatcgac cggtcgcgag gcggcgaaca gcgccgcggc
      781 gtggttgctg aagcggatga tgttgaagcg gtcgtgcggg ccgaggcgct gcacgccgag
      841 cgccagcgcg gccctggcct gggcgatgga cgggccgccc atggagccgg aggtgtcgat
      901 cacgaagatc acctcgcgcg gcggcggcgc gccggacggc gcctgcgggg gcgtcagcag
      961 caccagcgca tgcgtctcgc cgtcgaactg ctcggtgtag acggcggcgt gggtgtcggg
     1021 cgcgatcacc ggcgcccagg tcagctcgaa gtcgcgatcg gcggcggccc gcgcggcgag
     1081 aatgcggacc tgcccgccgg attcctcgct gctcaccggg tggtggtggc tggtgagcga
     1141 cgccagcggg aagcccgccg cgagctcgat ctcgatgtgc gcatggcccg aatccgcgct
     1201 gacgagctcc ggcgttgcgc agcgcgccgg caactcgccc tcggccgccg caatgcgcag
     1261 ttcgatggcg ccggctgcgc cgggttcggc gcccggcgtg tagcgcggcg tgatcgccag
     1321 cggcagccgc agcgtgtagt gcccgtcgcg ccagggaatg atctcgaggt aggcgatgcg
     1381 cacggtgatc tcgccacgcg gcgggatgtt ggcgacggcc gtagtgaaca tgttgggccg
     1441 cgcctggtcg accagcccgg cgcgccggcc ggcggcgcgg gccgtttcgt aggtcttgcg
     1501 ggcgatttcg cgtggctgga ttgcaccgtg gatcacacgg tcgccgacct cgagctcgag
     1561 ctggtccacg gccgcggtcg ccgccagcgg gaacacgtac agcccctcca tccagtcgtc
     1621 ggtgggattg ctgaaacgct gggtgagcgt gacgcgcgcc gtgttgccgg tgacccgcgc
     1681 gtggaatgcg ctggccatgc cgggcgccgc ctggggcgcg gcgtcggcgt cggctcgcag
     1741 ccagaggctg ccggcccctg cggcccacgg gtccactgcc gcatcggcag ggaaccacac
     1801 gatccccggc agcagcagcg ccgccagcgc cagtccccgc cgcgcgatct cgcgccagcc
     1861 gatgcgtccg gttcgcgcct tcctgtcgac tttcatctcg cccgctccct gtgagtggtg
     1921 gcagggagat tgggcgcctg cgaaagggcg ccgaccgggt cgcagtcagg cgaacccggg
     1981 agcaatcatg gcgaatggtg gcgggaaggc ggggaaaaac cggggtctga ccccggtttt
     2041 tcctggtttc aggtcacttc gttcagccgc ccgctggcga cgtcgtagac gaagccgcgg
     2101 accttgtccc ggtgcggcac gaagggactg ctcttgatgc ggcggacgga ctggcggatg
     2161 tcgtcggccg ggtcggcgaa agcctccagc gcgaacggcg ggcgcagccc ggtgtcggcc
     2221 tcgatctgcg ccttcagggc gtcgtccttg aaggtcacca tgccgcagtc ggtgtggtgg
     2281 atcagcacga tctcggtggt gcccagcagg cgctgcgaaa tcgccagcga gcggatgacg
     2341 tcgtcggtca ccacgccgcc ggcattgcgg atcacgtgcg cgtcgccttc ctccaggccg
     2401 agcaaccggc cggtctcgat gcgcgcgtcc atgcacgcca ccacggccac ccgcttggcg
     2461 ggcggcatcg gcttgtcgcc cttcgtgaac tgcgcggcgt aacggcggtt ggcggcgacg
     2521 aattcgtcgg tggctgacat cggtggtcct ccggggcggt tcacatggct gtcccgggga
     2581 cgctacctgc cggccggcgg agagggaaat acggaattgt taaggcgcta tgcctttgcg
     2641 gaatgtcgcc atttccgcac gccgctgacg accgccagca cgacggcgcc ggcgagcacg
     2701 ccgacgaggg cgttggacag cagcaagagc agcgcgccgc cgcccgtccc aaacccgttg
     2761 ccgagctgct cgagccagga ttcgagcccg gggacgccat gcgtgaggat gccgccgccg
     2821 accaggaaca tcgccgccgt gccggcgatg gcgaggcctt tcatcaggta cggcgcggcc
     2881 gccacgatgc cgcgcccgag cacctggacc gcgccctcgc cggggcgccg ggcaaggtgc
     2941 aggccgaagt cgtccagctt gacgatcgcc gccaccaggc cgtacacgcc gatggtcatg
     3001 agcacggcaa tggtcaccag caccattacg cgcgtgccga agggcgcact cgcgacgatc
     3061 cccagcgcga tgacgatgat ctcggcggac aggatgaagt cggtgcgcac cgccccgcgg
     3121 atcttgtcct tctccagcgc caccaggtcg atcgccgggt cggccagggc gtcggcgagc
     3181 tctgccgcat gcgcggcgcg ctcgcccttc tcgtgcagga acttgtgcgc cagcttttcg
     3241 cagccctcgt aacagaggaa cagcccgccg gccatcagca gcggcatgac ggcccagggc
     3301 gccagcgcgc tgatcgccag cgccgcgggc accaggacgg ccttgttgac gaaggagccg
     3361 cgcgccaccg cccagaccac cggcagttcg cggtcggcgc gcacgtcgct gacctgctgg
     3421 gcgttgagcg ccaggtcgtc gcccagcacg ccggcggttt tcttggcggt cgccttggtg
     3481 agcagggcga catcgtcgag caccgtggcg atgtcgtcga gcagcgcgag caggctggcc
     3541 ccggccatga gaaaaggggg tctgaccccg gtttcagcgc gcggcgcgca gcaattcgtt
     3601 gatgccgacc ttggcgcgcg tggcggcgtc tacgcgcttg acgatcaccg cgcaggcgag
     3661 atggcacttg ccctcgctgg acggcagcga accgggcacc accactgcac cggccggcac
     3721 ccggccatag
//
LOCUS       DAOMPG010000235         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10272_length_3698_cov_4.874005, whole genome shotgun sequence.
ACCESSION   DAOMPG010000235 DAOMPG010000000
VERSION     DAOMPG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10272_length_3698_cov_4.874005"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLS34_10990"
     CDS             <1..212
                     /locus_tag="PLS34_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRP88027.1"
                     /translation="VALQGNLDPAVLYASPEVIRHEVRQVLESFGRGSGHVFNLGHGI
                     HPEVQPEHAGAMIEAVVEFSPAYHR"
     gene            complement(190..954)
                     /locus_tag="PLS34_10995"
     CDS             complement(190..954)
                     /locus_tag="PLS34_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88028.1"
                     /translation="MGSGESRDVGARRLAEQLERSRLARLRQYDGESLRPECRLLAAW
                     QAARLRHTYRDLLEMSRYRKAVEFFLTDLYGDKDFSARDEGVERVYPIMSRVMPGPAL
                     LTISIGIELHAISQELDLDMVAMLWGEMGVGDRLDAQAYAEAYRRCGNVEARRRQIGI
                     VREVGESLDSVVFKPFIYNAVKLGRAPARMAGLLELHDFIERGFIAFRAMKGSEEFID
                     AIVTRETAIMEAILAGEPVTGWAPPGDSSVSGDRRG"
     gene            1088..1576
                     /locus_tag="PLS34_11000"
     CDS             1088..1576
                     /locus_tag="PLS34_11000"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRP88029.1"
                     /translation="MAKHNGTTPLIDNPLTRLVRDSASQAWSVGRGAYSFAGTEGGRL
                     VGSLLSFGGRIDRGAKSRVFEVRSSAGEAWDRLESAFVHRVARTLNSLQIPTARDVHE
                     LNARVEALQKAVVALERRAAETARPAGTARAARRKPAARRKSATRGTGKARPAARGKS
                     GS"
     gene            1581..2864
                     /locus_tag="PLS34_11005"
     CDS             1581..2864
                     /locus_tag="PLS34_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP88030.1"
                     /translation="MLQTQQTHLAEKSGGPKIGLALAGGGPLGGIYEIGALLALNEAI
                     DGLDFADVDVYVGVSAGSFVASTLANGISVEEMGRLLVSRRGRPQRFDPALFLQPHFI
                     EYLRRTAMLPRLLAEGVWEFLRNPLKQGVLESFSGLAKAIPTSVFDNQPIERFLADLF
                     SNRGMTNDFRRLPHKLLIVGVDLDTGEAVRFGSAGHDEVPISKAVQASTALPGLYPPV
                     KIGDRYFVDGGLKRTLHASSALDEGVDLLFCVNPIVPYDASAAPPGNDKRHDSLVEGG
                     LPVVLSQTFRSLIHSRMKVGMAKYETSYVDRDVVLFEPDTGDSKMFFTNVFSYANREM
                     VCQHAYRTTRRDLWQRRRRLAPILRRHGVVINEAILQDGERHFTSALSRRPDPKRLGH
                     YKNEVTNRLDALLDRLEIIVGPGAAGAVRGPADRA"
     gene            complement(2809..3324)
                     /locus_tag="PLS34_11010"
     CDS             complement(2809..3324)
                     /locus_tag="PLS34_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88031.1"
                     /translation="MNGRGQDGVPRNIPARRRAAWPAAVAGAACIFPALAGCTPHGEP
                     DGHAAARQPDLVKPRVPLQDPGAPVTNGEAQDLPPAEMLAPALADASRRSGVAEADLV
                     IEKSVRVTWNDGSLGCPQPGMSYTQALVSGWHLVIRAGERTFDYRGADRGRFMLCPPG
                     RGQPPQLRDLR"
     gene            complement(3321..>3698)
                     /locus_tag="PLS34_11015"
     CDS             complement(3321..>3698)
                     /locus_tag="PLS34_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898193.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRP88032.1"
                     /translation="DGFFKSGDVGIMDAEGFTRIVDRKKDMILVSGFNVYPNEVEGVV
                     VMHPAVLEAAAVGIPDEKSGEIVKLFVVCREGQSVPEKELIDFCRESLTGYKVPRLVE
                     FRKELPKTNVGKILRRELREGKA"
BASE COUNT          576 a         1290 c         1228 g          604 t
ORIGIN      
        1 gcgtggccct gcagggcaat ctcgaccccg cggtgctgta cgcgtcaccg gaggtaatcc
       61 gccacgaggt ccggcaggtg ctggagagct tcggccgcgg gtcgggccac gtgttcaatc
      121 tcggccacgg cattcatccc gaggtgcagc ccgagcacgc cggcgcgatg atcgaggccg
      181 tcgtcgagtt cagccccgcc tatcaccgct gaccgaggag tcgcccggcg gcgcccagcc
      241 ggtgaccggc tcgccggcca ggatcgcctc catgatggcg gtctcgcgcg tcacgatggc
      301 gtcgatgaat tcttccgaac ccttcatggc gcggaaggcg atgaagccgc gctcgatgaa
      361 gtcgtgcagc tccagcagcc cggccatgcg ggccggcgcg cggcccagct tcacggcgtt
      421 gtagatgaag ggcttgaata ctacgctgtc gagcgactcg ccgacctcgc gcacgatgcc
      481 gatctggcgg cggcgcgcct cgacgttacc gcagcgccgg taggcctcgg cgtacgcctg
      541 cgcatcgagc cggtcgccga cgcccatctc gccccacagc atcgctacca tgtcgaggtc
      601 cagttcctgg ctgatggcgt gcagctcgat gccgatgctg atggtcaaca gggcgggccc
      661 gggcatcacg cgcgacatga tggggtacac gcgctccacg ccttcgtcgc gcgccgaaaa
      721 atccttgtcg ccgtagagat ccgtgaggaa gaactccacc gccttgcggt agcgcgacat
      781 ctccagcagg tcgcggtagg tgtggcgcag gcgcgcggcc tgccaggcgg cgagcagccg
      841 gcactccggc cttaggcttt ccccgtcgta ctggcgaagc cgcgccagcc ggctgcgttc
      901 cagctgctcg gccagcctgc gcgcgccgac atccctgctt tctccgctgc ccatgggccg
      961 gcattgtagt gccaggccaa tgattttgct tgtgttaact gcgtatggaa tcggtccaaa
     1021 gatgactgtg accggcgcct gcgcggtggt atgctcagcc gatcaaacag ctgtttgagg
     1081 cacggacatg gcaaagcaca acggcacgac gccgctgatc gacaatccgc tgacccgcct
     1141 ggtcagggac tcggccagcc aggcctggag cgtgggccgc ggggcctact cattcgcagg
     1201 caccgagggc ggtcgcctcg tcggcagcct gctcagcttc ggcggacgca tcgatcgcgg
     1261 tgcgaagagc cgcgtcttcg aggtgcgcag ctcggcgggc gaagcctggg accgcctgga
     1321 gagcgcattt gtccaccgcg tggcgcgcac cctgaattcg ctgcagattc ccaccgcgcg
     1381 cgatgtccac gagctgaacg cccgcgtcga ggcgctgcag aaggccgtgg tggcgctgga
     1441 gcgccgtgcc gcggagactg caaggccggc gggcacggcg cgcgccgccc gtcgtaaacc
     1501 ggccgcccgc cgcaagtcgg cgacccgcgg caccggcaag gcgcgcccgg ccgcgcgcgg
     1561 gaagtccggc agctgagtcg atgctgcaga cccagcagac gcatcttgct gagaagtccg
     1621 gcgggccgaa gataggcctg gcgctggccg gcggcggacc gctgggcggg atctacgaaa
     1681 tcggcgcatt gctcgccttg aacgaggcca tcgacggcct ggacttcgcc gacgtcgacg
     1741 tctacgtcgg cgtgtcggcc ggctccttcg tcgcctcgac gctggccaac ggaatttcgg
     1801 tggaggagat gggccgcctg ctggtgagtc gccgcggccg gccgcagcgc ttcgacccgg
     1861 ccctgttcct gcagccccac ttcatagagt acctgcggcg caccgccatg ttgccgcggc
     1921 tgctcgccga aggtgtctgg gagttcctgc gcaatcccct gaagcagggc gtgctggagt
     1981 ccttcagcgg cctggccaag gcaatcccca cgtcggtgtt cgacaaccag cccatcgagc
     2041 gcttccttgc ggacctgttc agcaatcgcg gcatgaccaa cgatttccgc cggctgccgc
     2101 acaagctgct catcgtgggc gtcgacctgg acaccggcga ggcggtgcgg ttcggctcgg
     2161 cgggtcatga cgaggtgccc atttccaagg cggtgcaggc gagcacggcg ctgccgggac
     2221 tgtacccgcc ggtgaagatc ggcgaccgct acttcgtgga cggcggactg aagcgtaccc
     2281 tgcatgcgtc ctcggcgctc gacgagggcg tggacctgct gttctgcgtc aacccgatcg
     2341 tgccctacga tgcgagcgcg gcgccgcccg gcaacgacaa gcggcacgat tcactcgtgg
     2401 aaggtggcct cccggtcgtg ctgtcgcaga ccttccgttc gctgatccac tcgcgcatga
     2461 aggtcggcat ggccaagtac gagaccagtt acgtggatcg cgacgtggtg ctgttcgagc
     2521 cggacaccgg cgactcgaaa atgttcttca ccaacgtgtt cagctacgcc aaccgcgaga
     2581 tggtctgcca gcacgcctat cgcaccaccc ggcgcgacct gtggcagcgc cgccgccgcc
     2641 tcgccccgat tctcaggcgg cacggcgtgg tcatcaacga agccatcctg caggacggcg
     2701 agcggcactt caccagcgcc ctgtcgcgcc gtcccgaccc gaagcggctc ggtcactaca
     2761 agaacgaggt caccaaccgg ctcgatgccc tgctcgaccg cctcgagatc atcgtaggtc
     2821 ccggagctgc gggggctgtc cgcggcccgg cggacagagc atgaaccggc cgcggtccgc
     2881 gccccggtag tcgaaggtcc gttcccccgc ccggatgacg aggtgccagc ccgacaccag
     2941 cgcctgcgta taactcatgc ccggctgggg acagccgagc gagccgtcgt tccaggtcac
     3001 gcgcacggat ttctcgatca ccaggtccgc ttcggccacg ccgctgcgac gactggcgtc
     3061 ggcgagcgcc ggcgccagca tctccgccgg cggcaggtcc tgtgcctcgc cgttggtcac
     3121 gggcgccccc ggatcctgca gcggcacgcg gggcttgacg aggtccggct gccgcgcagc
     3181 ggcgtggccg tccggttccc catgcggcgt gcagcctgcc agcgccggga agatgcacgc
     3241 cgcaccggcc acggctgcag gccacgcggc gcggcgacgg gccgggatgt tgcgtggtac
     3301 gccatcttgc ccgcggccgt tcaagccttg ccctcgcgca gttccctgcg caggatcttg
     3361 ccgacgttgg ttttcggcaa ttccttgcgg aattccacca gtcgcgggac cttgtagccg
     3421 gtcaggctct cgcggcagaa gtctatgagc tccttttccg gcacactctg cccctcgcgg
     3481 cagaccacga acagcttgac gatttctccc gacttttcat cgggaatgcc gaccgcggcc
     3541 gcctcgagca cggccggatg catcacgacg acgccttcga cctcgttcgg atacacgttg
     3601 aagcccgaca cgaggatcat gtccttcttg cgatcgacga tgcgcgtgaa gccctcggcg
     3661 tccatgatgc cgacgtcacc cgatttgaag aagccgtc
//
LOCUS       DAOMPG010000236         3684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10329_length_3684_cov_3.836319, whole genome shotgun sequence.
ACCESSION   DAOMPG010000236 DAOMPG010000000
VERSION     DAOMPG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10329_length_3684_cov_3.836319"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="gspL"
                     /locus_tag="PLS34_11020"
     CDS             complement(<1..1142)
                     /gene="gspL"
                     /locus_tag="PLS34_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HRP88033.1"
                     /translation="MAETLVLRLDPADHGVGTWIVADDQGRRIGAPGAGPIGAAASAA
                     AGRRVFVLVPAVDVLLTSVTLPVRGTTKVLRALPFALEEQIAEDIEALHFAAGRVAAD
                     GKVTAAAVDREQLEAWLAAQLICSEAEGCPAAPNHLNWLLDADRCLARSGDGLPVLLE
                     IASVEEAMRFGPGFPGDGGEARHLSVYVTPEGRMRHGEALEAMREELASLELRILPDG
                     VLPHLAAGVISREPINLLQGAYAPRTQLDRLWKPWRAAAALLAVLAVVMVGQQALRLV
                     QLKQEEARLDAAIAEAFQQALPGARMEDPRFQVERRLAVLRGTGSTANESFLTALEAL
                     GGALGETPGITLQSVSFRAGVLDLRLLAPSVDSLERIRQSVGRDGR"
     gene            complement(1156..2079)
                     /gene="gspK"
                     /locus_tag="PLS34_11025"
     CDS             complement(1156..2079)
                     /gene="gspK"
                     /locus_tag="PLS34_11025"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HRP88034.1"
                     /translation="MQCRPRQRGAALITAILVVALATALAAALTWDLFLDQRRAFGRV
                     AVDQGLMYGLGAEAWARQILRDDGQDNQVDHPGEGWATRITPLPIEGGQIQGRIEDLN
                     GRFNLNNLIDATGATDPVAVEAFRRLLIGLELDVRLADRMADWIDRDVEPNFPDGAED
                     SEYLSRQPPFRTANRPVTSVSEIMLVYPELGRSGYDRLRPYVTALPRGTLININTALP
                     EVLLTASESMRISDAERIMFDRPNEGWDSLEQLGDMVPEDARGALGVTSNWFRLAVRV
                     DIGTQRFTMYSLLHRDASGSVSTVIRSFTDE"
     gene            complement(2081..2680)
                     /gene="gspJ"
                     /locus_tag="PLS34_11030"
     CDS             complement(2081..2680)
                     /gene="gspJ"
                     /locus_tag="PLS34_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HRP88035.1"
                     /translation="MRRARGFTLLELLVAMAIFAVVGLAAYGGLQAVLAQQVLARENA
                     DRFREIQFAVQQLSRDIYQAAPRPVRETLGDGARGALLGDARNRYPIEFTRGGWSNPL
                     GQPRAAVQRVAYELEDDRLLRRHWFVPDHTLDEEPVERELLTGVLEFRLRFLGAGGWS
                     DQWPPGLDATDPAMLPMAVEFVLELEDWGEIRRLVEVAN"
     gene            complement(2680..3183)
                     /gene="gspI"
                     /locus_tag="PLS34_11035"
     CDS             complement(2680..3183)
                     /gene="gspI"
                     /locus_tag="PLS34_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HRP88036.1"
                     /translation="MKRRRQRGFTLLEVMVAMAIVSIGLIAVFNAIIQMAHSTSTLRE
                     RALADWIAMNQISEIRLSGEFPDVGNFNDTVEFAGLPWRWEARISETGVENLRRIDMA
                     VAREETPDDAVTVMAGFVSRGVSGPALRVDWWGVGQPPAGPGEQEREPDPQAPGDQAP
                     REEEEED"
     gene            complement(3180..>3684)
                     /gene="gspH"
                     /locus_tag="PLS34_11040"
     CDS             complement(3180..>3684)
                     /gene="gspH"
                     /locus_tag="PLS34_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HRP88037.1"
                     /translation="ELLVVIVILAIMVGMTVLSLGTLRAEDPAETEANRLRALLSLVA
                     EEALIQGRDLGVEFFPDGYRFLSWDPDAALWSVIDDEAALRRRTLPEGLRVVLAVEGR
                     EVLIDDEERGRNRRQDELLPHVGVFSSGELTPFELFLVTDFIADAWLLRGRVQGDVEV
                     LAPEDVR"
BASE COUNT          574 a         1328 c         1250 g          532 t
ORIGIN      
        1 aaccggccgt cgcgcccgac cgactgccgg atccgctcca gtgagtcgac actcggcgcc
       61 agcagccgca ggtcgagcac cccggcccgg aagctgaccg actggagcgt gatcccgggc
      121 gtctcgccga gcgctccgcc gagcgcctcg agcgcagtga ggaaactctc gttggcggtg
      181 ctgccggtgc cacgcaagac cgccagccgt cgctccacct ggaagcgcgg gtcttccatg
      241 cgcgcgcccg gcaaggcctg ctggaacgcc tcggcgatcg cggcatcgag gcgcgcttcc
      301 tcctgcttga gctggaccag ccgcagtgcc tgctgcccga ccattaccac cgccagcacg
      361 gccagcaatg ccgccgccgc gcgccacggc ttccacaggc gatcgagttg cgtgcgcggc
      421 gcataggcgc cctgcagcag gttgatcggc tcgcggctga tgacgcccgc cgcgaggtgg
      481 ggcaggacgc cgtcgggaag aatacgcagc tcgaggctgg cgagttcctc ccgcattgct
      541 tccagcgcct cgccatggcg catgcggccc tcgggcgtga cgtacacgga caggtgtcgg
      601 gcctcgccgc cgtcgccggg gaagccgggg ccgaagcgca tcgcttcctc gacactggcg
      661 atctcgagca gcacgggcaa cccgtcacca ctgcgcgcca ggcagcgatc ggcgtcgagc
      721 agccagttga gatgattagg cgcggccggg cagccctcgg cctcggagca tatgagctgc
      781 gcggcgagcc aggcctccag ctgctcgcgg tccaccgcgg ctgccgtgac cttgccgtct
      841 gcggccacgc ggccggcggc gaaatgcagc gcctcgatgt cctcggcgat ctgctcctcg
      901 agcgcaaagg gcagggcgcg cagcaccttg gtcgtgccgc gcacaggcag cgtgacgctg
      961 gtgagcagca catcgacggc cggcaccagc acgaataccc tgcggcccgc ggccgccgac
     1021 gccgccgcac caatcggccc ggcgccgggc gcgccgatgc gacggccctg gtcgtcggcc
     1081 acgatccagg tgcccacgcc gtggtcggcg ggatcgagtc tcaggacaag ggtttcagcc
     1141 atggatttcg cgattctact cgtctgtgaa gctgcggatg acggtggaca ccgagccgga
     1201 tgcatcgcgg tgcaggagac tgtacatggt gaaccgctgg gtgccaatat caacccgcac
     1261 cgccaggcgg aaccagttgc tggtcacgcc aagcgcgccg cgcgcgtcct cggggaccat
     1321 gtcgccgagc tgctccagcg aatcccagcc ctcgttcgga cgatcgaaca tgatgcgttc
     1381 ggcgtcactg atgcgcatgc tctccgaagc ggtgagcagc acttccggca gggcagtatt
     1441 gatgttgatc agcgtgccgc ggggcagggc ggtgacgtag ggccgcagcc ggtcgtaacc
     1501 ggaccggccg agttccgggt agacgagcat gatctccgag acgctcgtca ccggccggtt
     1561 ggcggtgcgg aacggcggct ggcgggacag gtactcggaa tcttccgcgc cgtccgggaa
     1621 gttgggctcg acgtcccggt cgatccagtc ggccatgcga tccgccaggc gcacgtccag
     1681 ttccaggcca atgagcaggc gccgaaaggc ctcgacggcg accgggtcgg tcgcgcccgt
     1741 cgcgtcgatc aggttgttca ggttgaagcg gccgttgagg tcctcgatgc gcccctggat
     1801 ctgtccgccc tcgatcggca gcggggtgat gcgcgtcgcc cagccttcgc cggggtgatc
     1861 gacctggttg tcctggccgt cgtcgcgcag gatctgccgc gcccaggcct cggcgccgag
     1921 accatacatc agcccctggt ccaccgcgac gcgtccgaag gcgcgccgct ggtcgagaaa
     1981 caggtcccag gtcagcgctg cggccagcgc ggtggccagc gccaccacca ggatcgcggt
     2041 aatcagcgcc gcgccgcgct gtcgtggcct gcactgcatg tcagttggcg acctcgacca
     2101 ggcggcggat ttcgccccag tcctcgagct cgagcacgaa ttcgacggcc atcgggagca
     2161 tggccgggtc ggtggcatcg aggcccggcg gccactggtc gctccagccg cccgcgccga
     2221 ggaagcgcag ccggaactcg agcacgcccg tcagcagctc gcgctcgacc ggctcctcgt
     2281 ccagcgtgtg gtccggcacg aaccagtggc gccgcagcag ccggtcgtcc tcgagctcgt
     2341 aggccacgcg ttgcacggcc gcgcgcggct gccccagcgg gttgctccag ccgccgcgcg
     2401 tgaactcgat gggataccgg ttgcgggcgt cgccgagcaa ggcgccgcgc gcgccgtcgc
     2461 cgagcgtctc gcgcaccggg cggggcgccg cctggtagat gtcgcgactg agctgctgca
     2521 cggcgaactg gatctcgcgg aagcgatccg cgttctcgcg cgccagtacc tgttgcgcca
     2581 gcacggcctg cagtccaccg taggccgcca agccgaccac ggcgaaaatc gccatggcaa
     2641 ccagcagctc gagcagggtg aagccgcgtg cccggcgcat cagtcctctt cctcctcttc
     2701 gcgcggcgcc tgatcgcccg gtgcctgcgg atccggctcg cgctcctgtt cgccggggcc
     2761 ggcgggcggt tgcccgaccc cccaccagtc gacccgcagc gccggcccgc tgacgccgcg
     2821 ggacacgaaa cccgccatga cggtgacggc gtcatccggg gtttcctcac gggccaccgc
     2881 catgtcgatc ctgcgcaggt tctcgacccc ggtctcggaa atgcgggcct cccagcgcca
     2941 cgggagcccc gcgaactcca cggtatcgtt gaaattaccg acatcgggga actcgccgga
     3001 gagccggatc tcgctgatct ggttcatggc gatccagtcc gccagcgcgc gttcgcgcag
     3061 cgtcgaggtc gaatgcgcca tctggatgat ggcgttgaac accgcgatca ggccgatcga
     3121 gacgatggcc atggcgacca tcacctcgag cagggtgaac ccgcgctgcc gccggcgctt
     3181 caccgcacgt cctccggcgc gaggacctcg acgtctccct gtacgcggcc gcgcaacagc
     3241 caggcgtcgg cgatgaaatc ggtcaccagg aacagctcga aaggcgtcag ttcgccactc
     3301 gaaaacacgc ccacgtgcgg caggagttcg tcctggcgac ggttgcggcc gcgctcctcg
     3361 tcatcgatca gcacctcgcg tccctcgacc gcgagcacca cgcgcagccc ttccggcagc
     3421 gtccggcggc gcagcgcggc ctcgtcgtcg atcaccgacc agagcgccgc atcggggtcc
     3481 caggacagga aacggtagcc gtcgggaaag aactccactc cgaggtccct gccctggatg
     3541 agcgcttcct cggcgaccag cgacaacagg gcccgcaagc ggttcgcctc ggtctcggca
     3601 gggtcctcgg cgcgcagcgt gccgagcgac agcaccgtca tgccgaccat gatcgcaagg
     3661 atcacgatga cgaccagcag ctcg
//
LOCUS       DAOMPG010000237         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10518_length_3627_cov_4.376820, whole genome shotgun sequence.
ACCESSION   DAOMPG010000237 DAOMPG010000000
VERSION     DAOMPG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10518_length_3627_cov_4.376820"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLS34_11045"
     CDS             <1..1373
                     /locus_tag="PLS34_11045"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRP88038.1"
                     /translation="NVGDNVGDCAGMAADLFETYAVTIIASMLVAGIAFGTYGWSGVV
                     YPLVLGSASIIASIIGTFFVKARPGGKIMNALYRGLGVSGVLAAVAFWFITHSMFDAA
                     LAMPLFWCTIVGLVLTALLVVITEYYTATEYAPVRQVAKASETGHGTNVIAGLAVSLK
                     STAAPVIAVAAAIWFCYDLAGLYGLAVAATAMLSMAGMIVALDAYGPITDNAGGIAEM
                     SGLPPEVRETTDALDAVGNTTKAVTKGYAIGSAGLAALVLFADYAHKLDTSGTAVNFS
                     IDNPNVVIGLLIGGMIPYLFGALAMQAVGRAAGAVVEEVRRQFREIKGIMEGTGKPQY
                     DKAVDLLTRAAIKEMIVPSLLPLVIPIIVGLTLGADALGGLLMGTIVTGLFLAISMCT
                     GGGAWDNAKKYIEEGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKVTNIVAL
                     LLVPVL"
     gene            1655..2725
                     /locus_tag="PLS34_11050"
     CDS             1655..2725
                     /locus_tag="PLS34_11050"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRP88039.1"
                     /translation="MSTRTFLRRWRLPELLAAIATVAVASLAFAQSDTGVGRGDGLQP
                     GEFLWHPEVAPAGPVVLVVSLDEQRAYVYRNGIAIGVSTISSGKAGKETPPGVFTILQ
                     KKREHYSNLYDNAPMPYMQRLTWDGIALHGGRLPGYPASQGCVRLPQEFAAKLFTVTR
                     TGDTVVVAEGKSSAASLVHPAVLAPIAGTGEPAPATLRVGDAPTWDESLSPEGPVSVL
                     VSLADQRIFVLRNGVAIGEAPLQVEPGFGIGGTVLMVMGSETERVASLLDPARDRHRW
                     SAYPIHGLDAGALAMLQLQLQGQPLRADADFARQVRGVLAPGATVLLTDLPAVRAPLA
                     GSPLEPLLESGPEASAPVADGD"
     gene            2861..3541
                     /locus_tag="PLS34_11055"
     CDS             2861..3541
                     /locus_tag="PLS34_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRP88040.1"
                     /translation="MTEALARSAPALDRGVLDLAARAMSCALRQPGAALRPRTLSVID
                     FSRPSTEPRLWVFDLERPGLLFEEWVAHGRNTGGNMATEFSNRSGSYMSSLGGFQTQE
                     TYVGANGYSLRLRGLEPGFNDLARERAIVIHGAPYVNPLVAGTQGRLGRSLGCPAVRP
                     AVARELIDSISDGSFMFAYFPDEEWLEKSALLADDCGTLQQAMAASAARPREQGAEYL
                     FQEMALPD"
BASE COUNT          524 a         1295 c         1227 g          581 t
ORIGIN      
        1 acaacgtcgg cgacaacgtc ggcgactgcg ccggcatggc ggccgacctg ttcgaaacct
       61 atgcggtcac catcatcgcc tcgatgctgg tggcgggcat cgccttcggt acctatggct
      121 ggtccggcgt ggtgtacccg ctggtgctgg gctcggcctc gatcatcgcc tcgatcatcg
      181 gcaccttctt cgtcaaggcg cggccgggcg gcaagatcat gaacgcgctg taccgtggcc
      241 tcggcgtctc cggcgtgctg gcggcggtcg cgttctggtt catcacccac agcatgttcg
      301 acgccgcgct ggcgatgccg ctgttctggt gcaccatcgt cggcctggtg ctgaccgcgc
      361 tgctggtggt gatcaccgag tactacaccg cgaccgaata cgcgccggtg cggcaggtcg
      421 ccaaggcctc ggaaaccggc cacggcacca acgtcatcgc cggcctggcg gtgtcgctga
      481 agtccaccgc ggccccggtc atcgcggtcg ccgcggcgat ctggttctgc tacgacctcg
      541 ccggcctcta cggcctggcc gtggccgcca ccgcgatgct gtcgatggcc ggcatgatcg
      601 tggcgctgga cgcctacggt ccgatcaccg acaacgccgg cggcatcgcc gaaatgagcg
      661 gcctgccgcc cgaggtgcgc gagaccaccg acgcgctcga cgccgtgggc aacaccacca
      721 aggcggtgac caagggctat gcgatcggct cggccggcct cgccgcgctg gtgctgttcg
      781 ccgactacgc gcacaagctg gacacctccg gcaccgcggt gaacttctcc atcgacaatc
      841 cgaacgtggt catcggcctg ctgatcggcg gcatgattcc gtacctgttc ggcgcgctgg
      901 cgatgcaggc cgtgggccgc gccgccggtg ccgtggtcga ggaagtgcgc cgacagttcc
      961 gcgagatcaa gggcatcatg gagggcaccg gcaagccgca gtacgacaag gcggtcgacc
     1021 ttctgacccg cgccgcgatc aaggaaatga tcgtgccctc gctgctgccg ctggtgattc
     1081 cgatcatcgt cggcctgacg ctcggcgcgg acgcgctcgg cggcctgctg atgggcacca
     1141 tcgtgaccgg cctgttcctg gcgatctcga tgtgcaccgg cggcggtgcc tgggacaacg
     1201 ccaagaagta catcgaggaa ggccaccacg gcggcaaggg ttcggaagcg cacaaggccg
     1261 ccgtcaccgg cgacaccgtg ggcgatccct acaaggacac cgccggcccg gccgtgaacc
     1321 cgctgatcaa ggtcaccaac atcgtggcct tgctgctggt accggtgctc tgagccgcgc
     1381 aatcccgcac aacccatgac aacgcagccg ccccgcaccc gcggggccgg cctgcgacgg
     1441 ttgcgccctg caacccttca tgcgccccgg cttgccgggg ctttttttat ggcgtatcgc
     1501 tcgggtggca tgaggcgaaa gcaaggtcgg cgcgcggccg gagcgtccgt cgcgccatgt
     1561 gtggcgcgtg aagatgaacg cgacagcccc gcgttttccg cggaaaaggt actctgaccc
     1621 ccgtttcctt ttttcgcccg ccttggctgc acccatgtcc acacgcacgt tcctgcgccg
     1681 ctggcggctt ccggaacttc tcgccgccat cgccaccgtg gccgtcgcca gcctggcgtt
     1741 cgcccagtcc gacacgggcg tcggccgcgg cgatggactg cagcccggcg agttcctctg
     1801 gcatcccgag gtcgcaccgg ccgggccggt ggtgctggtg gtcagcctcg acgagcagcg
     1861 cgcctacgtc taccgcaacg gcattgcgat cggcgtgtcg acgatcagct cgggcaaggc
     1921 gggcaaggaa accccgcccg gcgtgttcac catcctgcag aagaagcgcg agcactactc
     1981 caacctctac gacaacgcgc cgatgccgta catgcagcgg ctgacctggg acggcatcgc
     2041 gctgcacggc ggccggctgc ccggctaccc ggcctcgcag ggctgcgtgc ggctgccgca
     2101 ggagttcgcg gcgaagctgt tcaccgtcac ccggaccggg gacacggtgg tggtggccga
     2161 aggcaagtcg tcggcggcca gcctggtgca tccggccgtg ctcgcgccga tcgcgggcac
     2221 cggcgaaccc gcgccggcca cgctacgcgt cggcgacgcg ccgacctggg acgaaagcct
     2281 ttcgcccgag gggccggtga gcgtgctggt cagcctggcg gatcagcgca tcttcgtatt
     2341 gcgcaacggc gtcgcgatcg gcgaggcacc gctccaggtc gagccgggct tcggcatcgg
     2401 cggcacggtg ctgatggtga tgggcagcga aaccgagcgc gtggccagcc tgctcgatcc
     2461 ggcccgcgac cggcaccgct ggagcgccta tccgatccac ggactcgacg ccggcgcgct
     2521 ggcgatgctg cagctgcagc tgcagggcca gccgctgcgg gcggacgcgg acttcgcccg
     2581 acaggtgcgc ggcgtacttg cgcccggggc cacggtcctg ctcacggacc tgcccgcggt
     2641 tcgtgcgccg ctcgccgggt cgcccctcga gccgctgctg gaatcggggc ccgaagcgtc
     2701 cgcccccgtg gcggatggag actgaatacg gccccggccg gcatcttgcg cggctgctat
     2761 ccttcccccg atgccctgtt ttcgccccct cgcgctgctg ctggcggtcg ccctcgcgat
     2821 cgccgacgct ggtgccacca atacctcgcg tctcgatccg atgaccgaag ccctcgcccg
     2881 cagcgcgccc gcgctcgatc gcggggtcct ggatctggcc gcgcgcgcca tgtcctgcgc
     2941 gctcaggcag cccggtgccg cgctgcggcc acggacgctg agcgtgatcg acttctcccg
     3001 gccctccacc gagccgcggc tctgggtctt cgacctcgag cgcccgggcc tgctgttcga
     3061 ggaatgggtc gcccacggcc gcaataccgg cgggaacatg gccaccgagt tctcgaaccg
     3121 ctcgggcagc tacatgtcca gtctgggtgg tttccagacc caggaaacct atgtcggggc
     3181 gaacggctat tcgctgcgcc tgcgtggtct ggaaccgggc ttcaacgacc tcgcccgcga
     3241 acgcgcgatc gtcatccacg gcgcgcccta cgtgaatccg ctcgtggccg gcacccaggg
     3301 ccggctcggg cgcagcctcg gctgcccggc ggtacggccg gcggtcgcgc gcgagctgat
     3361 cgattcgatc agcgacggtt ccttcatgtt cgcctacttc cccgatgagg aatggctgga
     3421 gaaatcggcg ctgctggccg atgactgcgg caccttgcag caggcgatgg cggcgtctgc
     3481 ggcgcggccg cgggaacagg gggcagagta ccttttccag gaaatggcgc tgcctgattg
     3541 accctcgtcc ctgaagacga ggccggatcg gctcggagcg cgcgccgatc cgccgtctgc
     3601 gcaaggcgtt gaaaccgcct cgaacgc
//
LOCUS       DAOMPG010000238         3618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10542_length_3618_cov_5.386472, whole genome shotgun sequence.
ACCESSION   DAOMPG010000238 DAOMPG010000000
VERSION     DAOMPG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10542_length_3618_cov_5.386472"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /gene="pcnB"
                     /locus_tag="PLS34_11060"
     CDS             complement(<1..1281)
                     /gene="pcnB"
                     /locus_tag="PLS34_11060"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRP88041.1"
                     /translation="MASSAPGRETQPRIIPRPEHNVSRSQISSNALKVLYRLRDAGYQ
                     AFLVGGGVRDLLLGREPKDFDIATDAHPEDVRRLFRNCRLIGRRFRLAHVYFGRDIVE
                     VATFRASDDHGEDDLDGDDESLLVKDEQGRILRDNRYGSIEDDIWRRDLTANALYYNI
                     ADFSIWDYAGGVEDIRAGVLRLIGDPEARYREDPVRLLRTVRFAAKLGFRIEPATEAP
                     LRSLAKSLEAVPPARLFDEVLKLFLSGHGVASYELLRRYGQFAQLFPAVDALLDSEAG
                     EPYRALLLEALQNTDQRVADDEPVTPVFLFGVLLWGPVRRLAARFEERGESQVQALLL
                     AADEVTDAQQRHVSIPRRITTPMKELMVMQLRLQRTRGRRVLGLLGQARFRAAYDFLC
                     LRARAGDADPALAEFWTELQAMDDDARAAAVGADG"
     gene            1419..1493
                     /locus_tag="PLS34_11065"
     tRNA            1419..1493
                     /locus_tag="PLS34_11065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Glu,seq:ctc)
     gene            1579..1857
                     /locus_tag="PLS34_11070"
     CDS             1579..1857
                     /locus_tag="PLS34_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88042.1"
                     /translation="MARLPATLTVLAGLLLTGCHTLSPEARQVVVLDRAADDCSHLGH
                     VNVDLTWTGLPSEAVIALRNKAADKGGNTLVLVSDHSGIAYRCPAARG"
     gene            1999..3603
                     /locus_tag="PLS34_11075"
     CDS             1999..3603
                     /locus_tag="PLS34_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRP88043.1"
                     /translation="MINEPAAPASAPHLPETRHPRGLYTLFFMEMWERFSYYGMRAIL
                     ILFLAAAVERGGLGIDIPTAGAIYGLYTAAVYLLALPGGWIADRLLGQQRAVWYGGIV
                     IAAGHFSMAVPGTATIFIGLTLIAVGTGLLKPNISAIVGELYPNDMGARRDAGFSIFY
                     MGINIGAFLGPLVCGWLGEGYSWHWGFGAAGVGMVFGLIQYRATQRHLGEAGAAPTPF
                     SDDPAIDAEKKRQVAMIAWGGAGLIALVVITGLLGVLPLNPIAIAANAGVTIVVLAAA
                     YFAWLLLRGGLSHLEKQRVGVIIILFVGAAMFWSGFEQAGSSLNLFAERHTDRMLFGW
                     EMPASWLQAVNPLFIITLAPFFAWFWVFLARRNLNPSIPAKFALGLIQLGLGFGVMAV
                     AATFAVKGETVLPTWLVLTYLLHTMGELCLSPVGLSAVTKLAPRRYVGQMMGTWFIAA
                     ALGNLMAGLIAGRFDPSALEQMPSLFTMVLMSTAGLGVVMLLFLRPFRALAAHQAPDV
                     VAGDRLFRVVGIVAVAIVGVAIFLTR"
BASE COUNT          511 a         1313 c         1222 g          572 t
ORIGIN      
        1 gccgtcggca cccaccgcgg cggcccgggc gtcgtcgtcc atcgcctgca gctcggtcca
       61 gaactcggcc agcgccgggt cggcgtcgcc ggcgcgcgcc cgcaggcaca ggaaatcgta
      121 agcggcccgg aagcgtgcct ggccgagcag gccgagcacg cgtcggccgc gggtccgctg
      181 cagccgcagc tgcatgacca tcagctcctt catcggcgtg gtgatgcgcc gcgggatgga
      241 aacgtgccgc tgctgcgcgt cggtcacctc gtcggccgcc agcagcagcg cctggacctg
      301 cgactcgccg cgctcctcga agcgcgccgc caggcggcgc accgggcccc acagcagcac
      361 gccgaacagg aacaccgggg tgacgggctc gtcgtcggcc acccgctggt cggtgttctg
      421 cagggcctcg agcagcagcg cgcggtaggg ttcgcccgcc tcgctgtcga gcagcgcgtc
      481 cacggccgga aacagctggg cgaactggcc gtagcgccgc aacaattcgt aggaagccac
      541 gccgtggccg ctgagaaaca gcttcagcac ttcgtcgaac agccgtgccg gcggcaccgc
      601 ctcgagcgat ttcgccagcg accgcagcgg cgcctcggtg gccggctcga tgcgaaagcc
      661 gagcttggcc gcgaagcgca ccgtgcgcag cagtcgcacc gggtcctcgc gatagcgcgc
      721 ctccgggtcg ccgatcagcc gcagcacgcc tgcgcggatg tcctccaccc cgccggcata
      781 gtcccagatc gaaaaatcgg cgatgttgta ataaagcgcg ttggccgtga ggtcgcggcg
      841 ccagatgtcg tcctcgatgc tgccgtagcg gttgtcgcgc aggatccggc cctgctcgtc
      901 cttcaccagc aaggactcgt cgtcaccgtc caggtcgtcc tcgccgtggt cgtccgaggc
      961 gcgaaaggtc gccacttcga cgatgtcgcg gccgaagtag acgtgcgcca ggcggaagcg
     1021 ccgcccgatc agccggcagt tgcggaacag ccggcggacg tcctcgggat gggcgtccgt
     1081 ggcgatgtcg aaatccttgg gctcgcggcc cagcagcagg tcgcgcacgc ccccgcccac
     1141 caggaaagcc tggtagccgg cgtcgcgcag ccggtacagc accttgaggg cgttgctgga
     1201 aatctgggag cgggaaacat tgtgctcggg ccgggggatg atccgcggct gtgtttccct
     1261 gcctggtgcc gagctggcca atccgtgtcc gcctctgtga gtcaagcccg caagtttatc
     1321 acgcagatcc ggccacgacg ccggggcatt gagtcgggcc gcgcggccca gtagaatgcc
     1381 cggcctcgcc gcgccccaag gcgcggccag cgcgcaacgc tccgttcgtc tagtggttag
     1441 gacgctggcc tctcacgccg gtaacagggg ttcgaatccc ctacggagcg ccactcccct
     1501 gtcgccgccc cgcctgacgc gggcacggcc acgcgggcaa gaggcgcctg ccagaccaac
     1561 cccattggag accgccccat ggccagactg ccagccacgc tcactgtcct cgccggcctg
     1621 ctgctcaccg gctgccacac gctcagcccc gaggcgcggc aggtggtcgt gctggaccgc
     1681 gcagccgacg attgcagcca tctgggacac gtcaacgtcg acctcacctg gaccggcctg
     1741 ccgtcggagg ccgtgatcgc cctgcggaac aaggccgcgg acaagggcgg caacaccctg
     1801 gtgctggtct ccgaccacag cggaatcgcc taccgctgcc cggcggccag gggctgacgc
     1861 gccggcaagg attcccctgc ggggcgccgc ccgacccggc ggtctcatcc gttttcgcac
     1921 cgcccgacat tgacgggatg tcacggttgc tgggaaagtc tcgcctcaaa tcgagcggac
     1981 acgcctgaaa ggggccccat gatcaacgag cctgccgcgc cggcgagcgc accccacctg
     2041 cccgagacgc gccacccccg gggcctctac acgctgttct tcatggagat gtgggagcgc
     2101 ttctcgtatt acgggatgcg cgccatcctg atcctgttcc tcgcggcggc cgtcgagcgc
     2161 ggcggcctcg gcatcgacat tcccaccgcc ggcgccatct acggcctgta cacggcggcc
     2221 gtctacctgc tggcactgcc gggcggctgg atcgccgacc ggctgctcgg ccagcagcgt
     2281 gcggtctggt acggcggcat cgtcatcgcc gccggccact tctccatggc ggtgccgggc
     2341 acggccacca tcttcatcgg cctgacgctg atcgccgtcg gcaccgggct cttgaagccg
     2401 aacatcagcg ccatcgtcgg cgagctgtac ccgaacgaca tgggcgcgcg gcgcgacgcc
     2461 ggcttctcga tcttctacat gggcatcaac atcggcgcct tcctcggccc gctggtgtgc
     2521 ggctggctgg gcgagggcta cagctggcac tggggcttcg gcgccgccgg cgtcggcatg
     2581 gtgttcgggc tgatccagta tcgcgccacc cagcgccacc tcggcgaggc cggcgcggcg
     2641 ccgacgccgt tcagcgacga cccggcgatc gacgcagaaa agaagcgcca ggtggccatg
     2701 atcgcctggg ggggcgccgg gctcatcgcg ctggtggtga tcaccggcct gctcggcgtg
     2761 ctgccgctca atcccatcgc catcgcggcc aacgccgggg tgaccatcgt ggtgctggcc
     2821 gccgcctact tcgcctggct gttgctgcgc ggcggcctga gccacctcga gaagcagcgc
     2881 gtcggcgtga tcatcatcct gttcgtcggc gcggcgatgt tctggtccgg cttcgagcag
     2941 gccggttcct cgctcaacct gttcgccgag cgccacaccg accgcatgct gttcggctgg
     3001 gagatgcccg ccagctggct gcaggcggtc aacccgctgt tcatcatcac gctggcaccg
     3061 ttcttcgcct ggttctgggt gttcctcgcg cgccgtaacc tcaacccgtc cattcccgcc
     3121 aagttcgcgc tcggcctgat ccagctcggc ctcggcttcg gcgtgatggc cgtggccgcg
     3181 accttcgcgg tcaagggcga gaccgtgctg cccacctggc tggtgctcac ctacctgctg
     3241 cacaccatgg gcgagctgtg cctgtcgccg gtcggcctgt cggcggtcac caagctggcg
     3301 ccgcgccgct acgtcggcca gatgatgggc acctggttca tcgccgccgc gctcggcaac
     3361 ctgatggccg gcctgatcgc ggggcgcttc gatccctcgg cgctggagca gatgccgagc
     3421 ctgttcacca tggtgctgat gagcacggcc ggactcggcg tggtgatgct gctgttcctg
     3481 cggccgttcc gcgccctcgc cgcccaccag gcgccggacg tggtggccgg cgaccggctg
     3541 ttccgcgtgg tcggcatcgt ggcggtggcg atcgtgggcg tggcgatctt cctgacgcgc
     3601 tgaacaggaa gccctgcg
//
LOCUS       DAOMPG010000239         3577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10707_length_3577_cov_3.844407, whole genome shotgun sequence.
ACCESSION   DAOMPG010000239 DAOMPG010000000
VERSION     DAOMPG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10707_length_3577_cov_3.844407"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PLS34_11080"
     CDS             <1..1124
                     /locus_tag="PLS34_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRP88044.1"
                     /translation="AVDLRAPVLAGSWTGPNRAIDHNGYPRGNRYYISNYTRGLTVLD
                     LTEPTAPVTVGHFDTFPLSDSPVFSGAWGVYPYLPSRVILVSDIESGLFVLRDGTLAS
                     DAGMLAFGNSSTGARSGATVQVPVVRGTGSSGPVSVAWEALPGSIGAADGPAAGGRLE
                     WPTGDAQPRFIELPVPELAADGPEVGRIFLRLFDPRGGAVLGAAALASVFVDLPGAAA
                     RLEFEDPPVMQEQRGRALLVVERRGSIAGEASMDFATRAGSAQAGEDFAAAAGTLSWG
                     DGDGTARVIEIELVADQVAEDAEQFFVDFSNPAGATVPRASATITIAASPGPPPPPPP
                     AAPAGGGGATGLLALLAICLCHLTRRGRPRYTRLPWRAG"
     gene            1168..1650
                     /locus_tag="PLS34_11085"
     CDS             1168..1650
                     /locus_tag="PLS34_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP88045.1"
                     /translation="MTQLDKTEAVGRSILGAELDSAERAALAAVMGVQPMKTGDVVVR
                     EGEPRRTLFVLADGKLSVCKGEGDHETCLYQLRIGECAGTRAFLDGSERKAKLRADSD
                     GALLTLEPEDFEQLIESHPRLVYKVMRGLFRVTHANLMRMNLETAELRNYMMKTGGRY
                     "
     gene            1655..2128
                     /locus_tag="PLS34_11090"
     CDS             1655..2128
                     /locus_tag="PLS34_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRP88046.1"
                     /translation="MTGSRVDDSPHIYVDADACPVKAEVVRVAVRHALPATFVANSGL
                     ALPRGPRVRLQVVSGAFDAADDWIVARLRANDIVVTADIPLASLALRKGARVLGPGGK
                     PFTDDNIGNALATRELMADLRACGVGGGPPPFGPRDRSRFLQALELTVREALRAG"
     gene            complement(2143..3111)
                     /locus_tag="PLS34_11095"
     CDS             complement(2143..3111)
                     /locus_tag="PLS34_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88047.1"
                     /translation="MAANQAANHAANHAAASAVDSSRYARCVEISKRIRWDIDRDVIC
                     GRKFDFSKKFLPEGLSQVGSMDFLSADEQRLLSQVQGRTYANIFGLVERFINAKVLEV
                     SRDHWLGDQVALEALVRFSDEELKHQELFRRIEEMIGAEMPAGYVLAADPNEVAAAVL
                     SKSTWAVLGLTCHIELFTQAHYRQSIEPESDVSELYKDVFLFHWREESQHAVLDELEW
                     RREHARLDAEARDRAVDDLIALVGAVDGILQAQARADAGYFARICGRPVGADETGRIE
                     AGLLRAYRWQYIVSGVQGERFREILGSLVTPAQGERIAAALAPIVN"
     gene            complement(3222..>3577)
                     /locus_tag="PLS34_11100"
     CDS             complement(3222..>3577)
                     /locus_tag="PLS34_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRP88048.1"
                     /translation="PQAPVVMFALEWCEFCWSVRRLFERLEVPCRVVSVDSVEFQAGD
                     RGGRIRAVLRRRTGAPTIPQVFIGGKLVGGCTDVFDAFKTGELQAQLAAAGVPLRDAG
                     EFDPYALLPGWLHAR"
BASE COUNT          529 a         1273 c         1213 g          562 t
ORIGIN      
        1 atgccgtcga cctgcgcgcg ccggtcctcg cgggctcctg gaccgggccc aaccgggcca
       61 tcgaccacaa cggctatccg cgcggcaacc gttactacat cagcaactac acccgggggc
      121 tgacggtgct cgacctcacc gagccgacgg cgccggtgac cgtcggccat ttcgacacct
      181 tcccgctgtc tgattccccg gtgttcagcg gcgcctgggg cgtctatccg tacctgccga
      241 gccgcgtgat cctggtgagc gacatcgaga gcggcctgtt cgtcctgcgt gacggcaccc
      301 tggcaagcga tgccggcatg ctcgccttcg ggaactcgtc gaccggcgcg cgcagtggcg
      361 cgacggtgca ggttccggtg gtccgcggca ccggctcgag cggccccgtg tcggtggcct
      421 gggaggcgct gccgggcagc atcggtgctg ccgacggccc ggcggcgggc ggccgactcg
      481 aatggcccac aggcgatgcc cagccccgct ttatcgagct gccggtcccc gagctggcag
      541 ccgacggacc ggaggtcggg cgcattttcc tgcgcctgtt cgacccgcga gggggcgccg
      601 tgctgggcgc cgcggcgctc gccagcgtgt tcgtcgactt gcccggggcc gctgcccgcc
      661 tggagttcga ggatcccccg gtcatgcaag agcagcgcgg ccgggccctg ctggtggtgg
      721 aacggcgcgg cagcatcgcc ggcgaggcca gcatggattt cgccacccgg gccggttcgg
      781 cgcaggcagg cgaggacttc gcagccgccg cgggtacgct cagttggggt gacggcgacg
      841 gcaccgcccg cgtcatcgag atcgagctcg tcgccgacca ggtagccgag gacgccgagc
      901 agtttttcgt cgacttcagc aaccctgcag gggcaactgt gccccgtgcg agcgccacca
      961 tcaccatcgc cgccagcccg gggcctcccc cgccgccgcc accggcggcg cccgctggcg
     1021 gcggtggcgc gacgggcctg ctcgccctgc tcgcaatctg cctgtgccac ctgacccgga
     1081 gaggccggcc gcgatacact aggctgccct ggcgggcggg atgaaagccc tggtaaccgt
     1141 gacgcgacct ggcgcgggga tgcgaccatg acacaactgg acaagaccga ggccgtcggc
     1201 cgctccatcc tgggcgccga gctggattcc gccgagcgcg ccgcgctggc ggcggtcatg
     1261 ggcgtgcagc cgatgaaaac gggcgacgtc gtcgtccggg agggagagcc gcggcgcacc
     1321 ctgttcgtgc tcgccgacgg caagctgagt gtctgcaagg gcgaaggcga tcacgagacc
     1381 tgcctgtacc agctgcgcat cggcgaatgc gccggcacgc gcgcctttct cgacggctcg
     1441 gagcgcaagg ccaagcttcg cgccgacagc gacggggcgc tgctgacgct cgagccggag
     1501 gacttcgagc agctcatcga aagtcatcca cgactggtct acaaggtgat gcgcggcctg
     1561 ttccgcgtca cgcatgccaa tctcatgcgc atgaacctgg agaccgccga gttgcgcaat
     1621 tacatgatga agaccggcgg gcgatattga ccccttgact ggttcccggg tcgacgattc
     1681 accgcatatc tacgtcgatg cggacgcctg cccggtcaag gccgaggtcg tgcgcgtggc
     1741 ggtgcgccac gccttgccgg cgaccttcgt cgccaattcc ggcttggcct tgccacgcgg
     1801 accgcgtgtc cggctgcagg tggtttccgg ggccttcgac gccgccgatg attggatcgt
     1861 ggccaggctt cgcgccaacg acatcgtcgt cactgcggac attccgctcg cctcgctggc
     1921 gttgaggaaa ggcgcgcgcg tgctcggccc cggcggcaag ccctttaccg acgacaatat
     1981 cggcaatgcc ctggccacgc gcgaactgat ggccgacctg cgcgcctgcg gcgtgggcgg
     2041 cggtcccccg cccttcggac cgagggaccg ctcccgattc ctgcaggcgc tggagctgac
     2101 cgtccgcgaa gctttgcgcg ccggctagcc tgcgccgccg cctcagttca cgatcggcgc
     2161 cagcgccgcg gcgatccgtt caccttgcgc cggagtcacc aggctgccca ggatttccct
     2221 gaagcgctcg ccctgcacgc cggagacgat gtactgccac cggtaggcgc gcagcaggcc
     2281 ggcctcgatg cggccggttt cgtcggcgcc gaccggccgg ccgcagatgc gcgcgaagta
     2341 gccggcgtcc gcccgcgcct gggcctgcag gatgccgtcg acggcgccca ccagcgcgat
     2401 caggtcatcg accgcccggt cacgcgcctc cgcgtcgagc ctggcatgct cgcgccgcca
     2461 ttcgagctcg tccagcacgg cgtgctgcga ttcctcgcgc cagtggaaca ggaacacgtc
     2521 cttgtacagc tcggacacgt ccgactccgg ctcgatgctc tggcggtaat gcgcctgggt
     2581 gaacagttcg atgtgacagg tcaggccgag caccgcccag gtcgacttcg acagcaccgc
     2641 ggccgctacc tcgttggggt ccgccgccag cacgtagccc gcgggcatct cggcgccgat
     2701 catctcctcg atgcggcgga acagctcctg gtgtttcagc tcctcgtcac tgaagcgaac
     2761 cagcgcctcg agcgcgacct ggtcgccgag ccagtggtcg cggctgacct ccagcacctt
     2821 ggcgttgatg aagcgctcca ccagcccgaa gatgttggcg taggtgcgcc cctgcacctg
     2881 actgagcagg cgctgctcgt cggccgacag gaagtccatg ctcccgacct gcgacagccc
     2941 ctcgggcagg aacttcttgc tgaaatcgaa cttgcgcccg cagatcacgt cgcggtcgat
     3001 gtcccagcga atgcgcttgg aaatttccac gcagcgcgcg tagcggctgc tgtcgacggc
     3061 actggcagcg gcatggttgg cggcatggtt ggcggcttgg ttggcggcca tagtgccggt
     3121 gtcccggatg gtagtggcat gcatgactct ctcctcggtc tgcttggaaa aaacctgggc
     3181 tccgtgttcg tgccccgcca gggggcgttg cctcgtgttg gtcaccttgc gtgcagccag
     3241 cccggcaaca gggcgtatgg gtcgaactcg cccgcgtcgc gcagcggcac gccggcagcg
     3301 gcgagctgcg cctgcagctc cccggtcttg aacgcgtcga agacatcggt gcagccgccc
     3361 accagctttc cgccaatgaa aacctgcgga atcgtcggcg cgccggtgcg acggcgcagt
     3421 accgcgcgga tccgcccgcc gcggtcgccg gcctggaact ccacggagtc caccgacacc
     3481 acgcggcacg gaacttccag gcgctcgaac aggcggcgca ctgaccagca gaattcgcac
     3541 cattccaggg cgaacatcac gaccggcgcc tgcggat
//
LOCUS       DAOMPG010000240         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10916_length_3522_cov_5.311797, whole genome shotgun sequence.
ACCESSION   DAOMPG010000240 DAOMPG010000000
VERSION     DAOMPG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10916_length_3522_cov_5.311797"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1278
                     /locus_tag="PLS34_11105"
     CDS             223..1278
                     /locus_tag="PLS34_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP88049.1"
                     /translation="MRAHSWIVGVVVVFAASLLGAGAVAAQDNVPKVTVLYDAFGKDP
                     ALEKDWGYAALIEVNGQRILFDTGNNGDILEKNARARNIDLSRLDFVVMSHRHGDHIG
                     GLAFLLEINPTVKIFAPKEGFGVFGADLPSSFYPKDPTLPAEQRYFDGEPPEVMRFGS
                     AWPAADFELIDKDTEIATNVHVIAQVSQKTGTLELRELSLAVNTDQGLIVLVGCSHSG
                     VENIVAAASKINPSIQLLAGGFHLIQGKAEELAAVITALRDTHQVAYVAPGHCTGELT
                     FTALTQAFGDRYLYAGLGTTFEIGDLVRAVEARNESVPSALDYDDFRELREHLAMSND
                     VEHILLARGIEHHHQAH"
     gene            1468..1992
                     /locus_tag="PLS34_11110"
     CDS             1468..1992
                     /locus_tag="PLS34_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRP88050.1"
                     /translation="MDRFSVVITAQDAERLRRLIATQRRGLLAAAAERLAAKLDGAVI
                     VRTTQVPDDVVTMYTRARIRHLDGDRSADYTLVLPGDAEIGSNRVSVLTPLGSALLGQ
                     REGDAVRWPKPDGGPRLRIEAILNQPEAVRGTRRRWPPRSWTVNGPARSVASHWDAGR
                     TARRSLPDGQANVA"
     gene            complement(2016..2924)
                     /gene="nhaR"
                     /locus_tag="PLS34_11115"
     CDS             complement(2016..2924)
                     /gene="nhaR"
                     /locus_tag="PLS34_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HRP88051.1"
                     /translation="MSTFRRLNFQHLLYFWSVVRAGSLTKACEQLNLSPPTISAQLRT
                     LEERLGEKLLTKSGRTLVPTEVGRMVFGYADEIFGLGRDLMEALEQRPTKRPLRLVVG
                     IDDVLPKEIAHHLLEPALKLSQSVRVLCREGTLERLVADLAVHEADVVLSDSPLTPSL
                     NIRAYNHHLGGCGVSWMAVPALAEALQPGYPGSLNGAPVLLPTDDTAIRRAVDQWLEK
                     HDIRPVIVGEFEDYALLREFARAGQGLAPVPSVLEEDFRSQSGLHLIGAAHSIQAKFY
                     AISMEKKIRHPAVVAICDAAREIFDK"
BASE COUNT          663 a         1156 c         1085 g          618 t
ORIGIN      
        1 caccgcaggt gcgagtcaat cccaccccct ccgccaaatt cactgacatc cctcatcggc
       61 gatcccggct gttcaagccg cgaagctgca ccccggaacc agtccggcca cccacccatc
      121 cggccataag caaatggcaa ttgattatta atcttttttc ggccgtgact ctagaatcgg
      181 gtcgctcaat accccgtgct gcccgtaccg ttggagatga tcatgcgagc ccattcctgg
      241 attgtcggcg tcgttgtcgt gttcgcggcg tccctgctgg gcgccggtgc ggtcgccgca
      301 caagacaacg tcccgaaagt gacggtgctc tacgacgcct tcggcaagga cccggcgctg
      361 gagaaggatt ggggctacgc cgcgttgatc gaggtcaacg gccaacgcat cttgttcgat
      421 accggcaaca acggcgacat cctcgaaaag aatgccaggg ccaggaacat tgacctgtca
      481 cggctggact tcgtggtgat gtcccaccgc cacggcgatc acatcggcgg cctggctttc
      541 ctgctcgaga tcaatccaac ggtcaagatt tttgcaccga aggaaggatt cggcgtattc
      601 ggcgccgatc tcccgagttc cttctatccg aaggatccga ccctgccggc ggaacaacgc
      661 tacttcgatg gcgagccgcc ggaggtgatg cgcttcggct cggcatggcc cgctgccgac
      721 ttcgagctga tcgacaagga cacggagatc gcgaccaacg ttcacgtcat cgcgcaggtc
      781 tcgcagaaga cgggcacgct ggagctgcgc gagctgtcgc tggccgtgaa cacggaccag
      841 ggcctgatcg tgctggtggg atgttcccac tcgggcgtgg agaacatcgt tgcagcggcg
      901 agcaagatca atcccagcat ccagttgctg gccggcggat tccacctgat ccagggcaag
      961 gcggaagagc ttgccgccgt gatcaccgcg ctgcgcgaca ctcaccaggt cgcttacgtc
     1021 gcgccgggtc actgcacggg cgagctgact ttcaccgcac tgacacaagc atttggcgat
     1081 cgctacctgt atgccggcct gggtacgacc ttcgagatcg gtgacctcgt ccgagctgta
     1141 gaggcaagga acgaaagcgt cccctcggcg ctggattacg acgacttcag agaacttcgc
     1201 gaacatctcg ccatgagcaa cgatgtcgag cacatcttgc tggccagggg aatcgaacat
     1261 catcaccagg cccactgacc ggcagctctc cgtccccagg tcgacctcaa gctgcacgat
     1321 ggaatacttc tataaacccg aatgattttg aagcaagttt ctaattgacc gcggaccccc
     1381 tgccgggtac aacaaagggg tgaaaatcgg cgggcagtgt gcccgtcttg gcggcaacag
     1441 ccgacccgcc tcgcctggag acccgatatg gaccgcttct ctgttgtcat tacggcacag
     1501 gacgcagaac gcctgcgcag actgattgcg acgcaacggc ggggcctgct tgccgcggcc
     1561 gcggagcgac tcgcggcgaa gctggatggc gcggtcatcg tcaggacgac ccaggtaccc
     1621 gacgacgtag tcacgatgta cacccgggcg cgcattcgcc acctcgacgg cgatcgctcg
     1681 gccgactaca ccttggtgct tcccggcgat gccgaaatcg gctcgaaccg cgtttccgtg
     1741 ctgacgccgc tcggcagcgc cttgctgggg cagcgcgaag gcgacgcagt gcgctggccg
     1801 aagccggatg gcggcccccg cctgcgcatt gaagcgatcc tgaatcaacc cgaggccgtg
     1861 cgcgggaccc ggcgccgctg gccgccgcga agctggaccg tgaacgggcc cgcccggagc
     1921 gtcgcctcac actgggacgc cggtcgaacg gcgcgcagga gcctgccgga cgggcaggcc
     1981 aacgtggcct gacggctcgt gccgcaacag cgcgtttact tgtcgaaaat ttcccgtgcc
     2041 gcgtcgcaga tggccacgac cgccgggtgg cggatcttct tttccatcga gatcgcgtag
     2101 aacttcgcct gtatgctgtg cgccgcaccg atgaggtgca ggcccgactg gctgcggaag
     2161 tcctcttcca ggaccgacgg caccggcgcc agcccttgcc cggcgcgcgc gaactcgcgc
     2221 agcagtgcgt aatcctcgaa ctcgccgacg atgaccggcc ggatatcgtg cttttcgagc
     2281 cactggtcga ctgcgcggcg gatcgccgta tcgtcggtcg gcagcagcac cggtgcaccg
     2341 ttcaaggaac ccgggtagcc gggttgcagc gcctctgcca acgccggcac cgccatccat
     2401 gaaacgccgc acccgccgag atggtggttg taagcgcgga tgttgaggct gggcgtcaag
     2461 ggcgagtccg acaacacgac atccgcttcg tggacggcaa gatcggcgac caggcgctcc
     2521 agcgttccct cccgacaaag gacacgcacc gactggctga gcttgagcgc aggttcgagc
     2581 aggtgatgcg caatttcctt cggcagtacg tcgtcgatgc cgaccaccag tcgcagcggc
     2641 cgcttggtcg ggcgttgctc gagggcctcc atcaggtccc gcccgaggcc gaagatctca
     2701 tctgcgtagc cgaataccat tcggccgact tccgtgggta ccagggtgcg cccggacttc
     2761 gtcaacagct tctcgccgag ccgctcctcc agcgtgcgca gctgcgcact gatcgtgggc
     2821 ggggacaggt ttagctgctc gcaagccttg gtaaggctgc cggcacgcac gaccgaccag
     2881 aaatacagca ggtgctgaaa gttcaacctt ctgaaagtac tcataagatt tcgtttttat
     2941 cgaatattcg cttgcagtaa ttctaatgga taaaagtaac tctgtcatgt ctaatagccg
     3001 tggtggttcg ttgccgggac tcgactgccc ccaagcgggg catggaccac atgctccggg
     3061 caacccgtcc gcagcatccg cgccgcagcc gatcctgcag cgacccagcc acggaaagaa
     3121 tggaatggcc gggaggggtg gagccaggct cctccccttc caaaggagga aacaatgcaa
     3181 tcggtcgtca gagtgcgggg cgatctgtgg aagcagaccc tgcgtgacag gcacggggcc
     3241 gagcaggccg agaatcaccg ctatgcccgc gcaccccgcc gtcgcggtcg acgtcggcgc
     3301 tcgatgcggt cctacggccg ggccacgcac cggccgcatt caccgttcgt cgttcgcttc
     3361 tgatctgcgc catggccggc tgcctgcagt cggatccctc ctgcccatga acggcaagag
     3421 cccggccccg catctgcggg gccgggctct tgccgtccag gccggcgact cgcctcaggc
     3481 gaggtcgaag cgatccgcgt tcatcacctt ggtccaggcc gc
//
LOCUS       DAOMPG010000241         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_10960_length_3508_cov_3.269041, whole genome shotgun sequence.
ACCESSION   DAOMPG010000241 DAOMPG010000000
VERSION     DAOMPG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_3508_cov_3.269041"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PLS34_11120"
     CDS             <1..1575
                     /locus_tag="PLS34_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP88052.1"
                     /translation="TSMFIRGANSNHTLVLIDGVKVNPGTAGGAALQNIVPELVERIE
                     IVKGPRSSLHGSEAIGGGINIITRRGGPGRELGARAQAGRYATRGLGGNVSWQEESFG
                     AGISASALRSDGFPTFRDSADDSAFENDVLNAWLRASADAFDFEASHWQAAGNSEYAD
                     FFRAPQDQDFTNRLSRLRLGWQGRDWRSTLTLSRFLDRIEQGALAFDPDDYAETERDV
                     VDWQNDLDFIAGLELTAGVMLSRERTAGQSFGTPLESAPGRGDADRDEEAVYLQLGTT
                     LGRHRLAAAGRHTDHGAFGGVNTWNLEWGMPLGDDWSVTAGLGRGFRAPGTSDLYAFG
                     GNPALQPEVSRSADLGITYRPTPDHALELALFRTEVTGLIEYFDPDGFTGPLPGRNEN
                     IGAARIKGAELTWRAFYGDWQVTTTMLTQRPEDRTTGEPLLRRAEQSATATLVRRIGR
                     HEAGLQVLAAGERYDFGGVRLAGYVLAGLTASYTISAHWTLRARLDNLLDQDIELVEG
                     YNSAGRGIYASLAYSY"
     gene            1595..2152
                     /locus_tag="PLS34_11125"
     CDS             1595..2152
                     /locus_tag="PLS34_11125"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRP88053.1"
                     /translation="MGHRLSRIYTRTGDDGTTGLGDGSRIPKDDARVEAFGTVDEANC
                     AVGLVLAVDGLPEDIVACLTRVQHDLFDLGGELCIPGYSAITAGYVARLERELDGFNA
                     DLPPLKDFILPGGGPAAAACHLARTVTRRAERRVWTLARRDALNPEAARYLNRLSDLL
                     FVLARVIARHEQGSEVIWQKGLNRD"
     gene            complement(2201..3046)
                     /locus_tag="PLS34_11130"
     CDS             complement(2201..3046)
                     /locus_tag="PLS34_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRP88054.1"
                     /translation="MNVRLALLAGLLWAAGCVAASPHAAAPGALPPAQRIVTLAPHLA
                     ELVFEAGAGDRLVGTVAWTDYPPAAAALPHIGDAFRIDLEALAALRPDLVLGWRGGNP
                     DHLLEQLAQRGYRVVALEPRKLDDIGRHLVQIGELAGTEAAAREAAASYRVDLYALRQ
                     AHAGKPELRVFYQVSWRPLYTVGGRQLISEIIALCGGRNLFDDLGELAPAVAMEAVIA
                     RDPQVILTAEGQSVDLEEWRRWPGVSAVAAGHLYTVDGDLVVRPSPRILEGTRQVCAA
                     LDLAR"
     gene            complement(3043..>3508)
                     /gene="ampE"
                     /locus_tag="PLS34_11135"
     CDS             complement(3043..>3508)
                     /gene="ampE"
                     /locus_tag="PLS34_11135"
                     /inference="COORDINATES: protein motif:HMM:NF028423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HRP88055.1"
                     /translation="LESAIFVQANHRIFAVIFWFMLLGPAGAWAYRVSDLFRRHVRAT
                     GTGGRATEVVELLFGLVAWIPARLLALSYAVAGSFDDAFEDWRAYYQRTTAGLFSVSE
                     NILSAAGIGAIRRRCPAGDPVSAVRSARELLRRALFVWITVIAVLTLVGWAA"
BASE COUNT          550 a         1289 c         1235 g          434 t
ORIGIN      
        1 acgtcgatgt tcatccgcgg cgccaacagc aatcacacgc tggtcctcat cgacggcgtc
       61 aaggtgaacc cgggcacggc cggcggcgcc gccttgcaga acatcgtccc ggagctggtc
      121 gagcgcatcg agatcgtcaa gggcccgcgc tcctcgctgc acggctcaga ggccatcggc
      181 ggggggatca acatcatcac gcggcgcggt ggccccggcc gcgagctggg cgcgcgcgcc
      241 caggcgggcc gttacgcgac gcgcggtctc ggcggcaacg tctcctggca agaggagagc
      301 ttcggcgccg ggatctcggc gagcgcgctg cgcagcgacg gctttccgac cttccgcgac
      361 agcgccgatg acagcgcgtt cgagaacgac gtgctcaacg cctggctgcg cgcctcggcc
      421 gacgccttcg acttcgaggc cagccactgg caggccgccg gcaacagcga gtacgcggat
      481 ttcttccgcg cgccgcagga ccaggacttc actaaccggc tgagccgcct gcggctgggc
      541 tggcaggggc gtgactggcg ctcgacgctg acgctgtcgc gtttcctcga tcgcatcgaa
      601 cagggcgcgc tggccttcga tccggacgat tacgccgaga ccgagcgcga cgtcgtcgac
      661 tggcagaacg acctcgactt catcgccggc ctcgagctca cggccggggt catgctgagc
      721 cgcgagcgca ccgcggggca gagcttcggc acgccgctgg aatccgcgcc gggccggggc
      781 gacgccgacc gcgacgagga agccgtctac ctgcagctgg gcacgacgct cggccggcac
      841 cggctggcgg ccgccggccg gcacaccgat cacggggcgt tcggcggcgt gaacacctgg
      901 aacctggaat ggggcatgcc gctgggcgac gactggtccg tgaccgccgg cctcgggcga
      961 ggcttccgcg cgccgggcac cagcgacctg tacgccttcg gcggcaaccc ggcgctgcag
     1021 cccgaggtgt cgcgcagcgc ggacctcggc atcacctacc gccccacgcc cgaccatgcg
     1081 ctggagctgg cgctgttccg caccgaggtc accggcctga tcgagtactt cgacccggac
     1141 ggcttcaccg gcccgctgcc cggccggaac gagaacatcg gcgcggcgcg catcaagggc
     1201 gccgagctga cctggcgcgc gttctacggc gactggcagg tcaccaccac catgctcacg
     1261 cagcgcccgg aggaccgcac gaccggcgag ccgctgctgc gacgggccga gcagagcgcc
     1321 accgccacgc tggtgcggcg catcggccgg cacgaggccg gactccaggt gctggccgcg
     1381 ggcgaacgct acgacttcgg cggggtgcgg ctggccggct acgtgctggc cggcctgacc
     1441 gccagctaca cgatctcggc gcactggacg ctgcgcgccc ggctcgacaa cctgctcgac
     1501 caggacattg aactggtgga aggctacaac tcggccgggc gcggcattta tgccagtctc
     1561 gcctacagtt actgaacagg acacggaagc actcatggga cacagactca gccgcatcta
     1621 cacccgcacc ggcgacgacg gcaccaccgg cctcggcgac ggtagccgca tcccgaagga
     1681 cgacgcccgc gtcgaggcct tcggcacggt cgacgaggcc aattgcgccg tcggcctggt
     1741 gctggcggtc gacgggctgc cggaggacat cgtcgcctgc ctgacgcgcg tgcagcacga
     1801 cctgttcgac ctcggcggcg agctgtgcat cccgggctac agcgccatca cggcgggcta
     1861 tgtcgcccgc ctcgagcgcg agctggacgg cttcaacgcc gacctgccgc cgctcaagga
     1921 cttcatcctg ccgggtggcg gcccggccgc ggctgcctgc cacctggcgc gcaccgtcac
     1981 ccggcgcgcg gagcggcggg tgtggacgct ggcgcggcgc gatgcgctca accccgaggc
     2041 ggcccgctac ctcaaccggc tgtccgacct gctgttcgtg ctggcgcgcg tcatcgcccg
     2101 ccatgagcag ggctcagagg tgatctggca aaagggcctg aaccgcgact gatggggaca
     2161 ctccccaact tcgcgaggcg aaattgggga gtgtccccat ttagcgggcc aggtcgaggg
     2221 cggcgcagac ctggcgggtg ccctccagga tgcgcggcga cggccgcacc accaggtcgc
     2281 cgtcgacggt atagagatgg cccgcggcca ctgcgctgac gcccggccag cggcgccact
     2341 cctcgaggtc cacggactgg ccttcggccg tcaggatcac ctgcggatca cgggcgatga
     2401 cagcctccat ggcgaccgcc ggcgccagct cgccgagatc atcgaacagg ttgcggccgc
     2461 cgcacagggc gatgatctcg ctgatgagct gccggccgcc gaccgtgtac agcggccgcc
     2521 aggacacctg gtagaacacc cggagctcgg gcttgccggc gtgggcctgg cgcagggcgt
     2581 agagatcgac gcggtagctg gcggccgcct cgcgcgccgc cgcctccgtg ccggccagct
     2641 cgccgatctg caccaggtgg cggccgatgt cgtccagctt gcggggctcc agcgccacca
     2701 cgcgatagcc gcgctgcgcg agctgctcga gcaggtggtc gggattgccg ccccgccagc
     2761 cgagcaccag gtccggccgc aaggccgcca gcgcctcgag atcgatgcgg aaggcgtcgc
     2821 cgatgtgcgg caacgccgcg gcggccggcg gatagtcggt ccaggccacg gtgccgacca
     2881 gccgatcacc cgccccggcc tcgaacacca gctcggcgag gtgcggcgcc agcgtgacga
     2941 tgcgctgcgc cggcggcagc gcacccggcg cggcggcgtg cggcgatgcc gcgacacagc
     3001 cggccgccca caacaagccc gcgagcaggg caaggcgcac gttcacgcgg cccagccgac
     3061 cagcgtcaac accgcgatca cggtgatcca gacgaacagg gcgcggcgca gcagctcgcg
     3121 tgccgaccgc acggccgaca ccgggtcgcc cgcagggcaa cgccgccgga tggcgccgat
     3181 gcccgctgcc gagaggatgt tctcgctgac gctgaacagc ccggcggtgg tccgctggta
     3241 atacgcccgc cagtcctcga aggcgtcgtc gaagctgccg gcgacggcat aggacagcgc
     3301 cagcaggcgc gccggaatcc aggccaccag cccgaacagc agttccacca cctccgtcgc
     3361 ccggccgccg gtgcccgtcg cgcgcacgtg gcggcggaac aggtcgctga cgcggtaggc
     3421 ccacgcgccc gcgggcccga gcagcatgaa ccagaagatc accgcaaaga tgcggtggtt
     3481 ggcctgcacg aagatggcgc tttccagc
//
LOCUS       DAOMPG010000242         3478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11073_length_3478_cov_5.816827, whole genome shotgun sequence.
ACCESSION   DAOMPG010000242 DAOMPG010000000
VERSION     DAOMPG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_3478_cov_5.816827"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="petA"
                     /locus_tag="PLS34_11140"
     CDS             <1..273
                     /gene="petA"
                     /locus_tag="PLS34_11140"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRP88056.1"
                     /translation="NRHRSITPEVLVVIGSCTHLGCSPMPRFEVGAADLGADWPGGFF
                     CACHGSKFDLAGRVWRGVPAPLNLVVPPHKYLDESTILVGVDTGAA"
     gene            363..1532
                     /locus_tag="PLS34_11145"
     CDS             363..1532
                     /locus_tag="PLS34_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HRP88057.1"
                     /translation="MMDEHVTGYYAPKNFNWYYVFGSLALLALIIQIVTGIFLTMSYK
                     PSAAEAFGSVEYIMRDVEWGWLIRYMHSTGASLFFVVVYVHMFRALLYGSYQKPRELI
                     WIFGMLIYLAMSAEAFFGYLLPWGQMSYWGAQVIVSLFGALPVIGEPLVEWIRGDYFI
                     SDVTLNRFFALHVIALPLVLLGLVVAHILALHEVGSNNPDGVEIKKYKGPDGKPLDGV
                     PFHPYHTTKDVPAWVVFSMVFAAIVFFAPEMGGYFLEHANFEPADPLKTPDHIAPVWY
                     FTPFYAILRAIPDKLGGVVMMGAAMFVLFLLPWLDRSPVRSIRYKGPLFKIALTVFTI
                     SFVMLGWLGLQPATGVYTTLARVFTVLYFAFFLLMPIYSKIDRTKTPPDRITYHG"
     gene            1525..2277
                     /locus_tag="PLS34_11150"
     CDS             1525..2277
                     /locus_tag="PLS34_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRP88058.1"
                     /translation="MDRRFQMPLLALLLTLPGLVFAAAPGVALQSSGADVSNQASLQR
                     GAQLYMNYCAGCHSLQYVRYNTLADGLGLSDEQVAENLRFTGTSPHEQIRIAMPAEDA
                     AGWFGLAPPDLSLIARARGTDYVFTFLKSFYADPGSRATGANNKVLAGTAMPHVLWEL
                     QGVQEAVYRQETGADGVTRDVFDRFELVRPGSMTPEEFDQAARDITAFLSWAAEPMQV
                     ERKRLGTLVLLFLLVLFAFSYLYYKELWKDVK"
     gene            2315..2914
                     /locus_tag="PLS34_11155"
     CDS             2315..2914
                     /locus_tag="PLS34_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP88059.1"
                     /translation="MTLFSNPTCPHSHRVRIVLAEKGISLDVVDVETASLPEDLLALN
                     PYNSVPTLVDRDLVLYDSRVIAEYLDERFPHPPLMPVDPVSRARFRLALYHIERDWYS
                     LVDDLERGDKRTANRARKNLRESLLSSAELFGVKPYFMSDEFSLVDCSIAPILWRLPR
                     WEVDLPPSHGRNIIKYAETLFSRPSFRTSLTEFEQEMRP"
     gene            2911..3327
                     /locus_tag="PLS34_11160"
     CDS             2911..3327
                     /locus_tag="PLS34_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HRP88060.1"
                     /translation="MTGRDQTRRPRRPYLLRAMVEWITDNGETPHIVVDAAQEGVQVP
                     SQYVTEGKVVLNIGGNATQGLSLGNEAVEFSARFGGVPFPVRVPLRAVLGIYARESGE
                     GMIFTDSEGEPPPGNDGGDGDSPGPQKGPPKLSVVK"
BASE COUNT          531 a         1175 c         1136 g          636 t
ORIGIN      
        1 aaccgccatc gctccatcac gcccgaggtg ctggtggtga tcggcagctg cacccatctc
       61 gggtgttccc ccatgccgcg cttcgaagtc ggcgcggctg acctgggcgc cgactggccc
      121 ggcggctttt tctgcgcctg tcacggttcg aagttcgatc tcgcgggccg cgtctggcgt
      181 ggtgtcccgg cgccgttgaa cctggtggtg ccgccgcaca agtaccttga cgagtcgacc
      241 atcctggtcg gcgtggacac gggggccgcc tgatgagtag cgcgacggac aagaaggcgg
      301 cggtcggcca gcaaggcggc gggctctacg actggttccg gaagcggttc ccctgggacc
      361 agatgatgga cgagcacgtc accggttact acgcgccgaa gaatttcaac tggtactacg
      421 tgttcggctc gctggcgctg ctggcgctga tcatccagat cgtcacgggc atcttcctga
      481 cgatgagcta caagccctcg gcggcggagg ccttcggctc ggtcgaatac atcatgcgcg
      541 acgtcgaatg gggctggctc atccgctaca tgcactcgac cggcgcctcg ctgttcttcg
      601 tggtggtcta cgtccacatg ttccgcgcgc tgctgtacgg gtcctaccag aagccgcgcg
      661 agctcatctg gatcttcggc atgctgatct acctcgcgat gtcggccgag gccttcttcg
      721 gctacctgtt gccgtggggc cagatgtcct actggggcgc gcaggtgatc gtgtcgctgt
      781 tcggcgcgct gccggtgatc ggcgagccgc tggtggaatg gatccgcggc gactacttca
      841 tctccgacgt gaccctgaac cgcttcttcg cgctgcacgt gatcgcgttg ccgctggtgc
      901 tgctcgggct ggtcgtggcg cacatcctgg cgctgcacga ggtcggctcc aacaaccccg
      961 acggcgtgga aatcaagaag tacaaggggc ccgacggcaa gccgctcgac ggggtgccgt
     1021 tccatcccta ccacaccacc aaggacgtgc ccgcctgggt cgtgttctcg atggtgttcg
     1081 ccgcgatcgt gttctttgcg ccggaaatgg gcggctattt tctcgagcac gccaacttcg
     1141 agccggcgga cccgctgaag acgccggacc acatcgcgcc ggtgtggtac ttcacgccct
     1201 tctacgcgat cctgcgggcg attccggaca agctcggcgg cgtcgtgatg atgggtgcgg
     1261 ccatgttcgt gctgttcctg ctgccctggc tcgatcgcag cccggtgcgc tcgatccgct
     1321 acaagggccc gctgttcaag atcgccctca cggtgttcac catcagcttc gtgatgctcg
     1381 gctggctggg cctgcagcca gccaccggcg tctacacgac gctggcccgc gtgttcacgg
     1441 ttctttactt cgcgttcttc ctgctgatgc cgatctactc gaagatcgac aggacgaaga
     1501 cgcctccgga caggatcacc taccatggat aggcgcttcc agatgccgtt gctcgccctg
     1561 ctgctcacgc tgccgggcct ggtcttcgct gccgccccgg gcgttgcgct ccagtcgtcg
     1621 ggcgccgacg tgagcaacca ggcgtccctg cagcgtggcg cccagctgta catgaactac
     1681 tgcgccggct gccattcgct gcagtacgtc cgctataaca cgctcgccga tggcctcggc
     1741 ctgagcgacg agcaggtggc ggagaacctg cgcttcaccg gcaccagccc gcacgagcag
     1801 atccggatcg caatgccggc ggaggacgcc gccggctggt tcgggctggc gccgccggac
     1861 ctgtcgctga tcgcgcgcgc gcgcggcacc gactacgtgt tcaccttcct gaagtccttc
     1921 tacgccgatc ccggttcgcg cgcgaccggc gccaacaaca aggtgctggc gggtacggcc
     1981 atgccgcacg tgctgtggga gctgcaggga gtccaggagg ctgtttaccg gcaggagacc
     2041 ggcgccgacg gggtcacccg ggacgtgttc gatcgcttcg agctggtccg gcccgggtcc
     2101 atgacgccgg aggagttcga ccaggccgcg cgcgacatca ccgccttcct gtcctgggcg
     2161 gcggagccga tgcaggtcga gcgcaagcgc ctcggcacac tcgtgctcct gttcctgctc
     2221 gtgctgttcg cgttctccta cctgtactac aaggaactct ggaaggacgt gaagtgatct
     2281 tccggggcgc ggcgttgacc gcaggcgagg ccacatgact ctgttttcga atccgacctg
     2341 cccgcacagc caccgcgtgc gcatcgtgct ggccgagaaa ggcatcagcc tcgacgtcgt
     2401 ggacgtggaa accgccagcc tgcccgaaga cctgcttgcg ctgaacccgt acaacagtgt
     2461 gccgacgctg gtcgaccggg acctcgtgct gtacgactcg cgggtcattg cagagtacct
     2521 ggacgaacgt tttccccatc cgccgctgat gccggtcgac ccggtcagtc gtgcccgctt
     2581 ccggctcgcg ctgtatcaca tcgagcgcga ctggtactcg ctggtggatg acctcgagcg
     2641 cggcgacaag cgcaccgcga accgtgcgcg caagaacctg cgcgaaagcc tgctctccag
     2701 cgccgagctg ttcggcgtca agccttattt catgagcgac gagttttcgc tggtcgactg
     2761 cagcatcgcg ccgatcctgt ggcgcctgcc gcgctgggaa gtcgacctgc cgcccagcca
     2821 cggccgcaac atcatcaagt acgccgagac cctgttcagt cgcccgtcgt tccgcacgag
     2881 cctcaccgag ttcgaacagg agatgcgccc ctgaccggtc gcgaccagac gcgccggccg
     2941 cgccggccgt acctgctgcg cgccatggtc gagtggatca cggacaacgg cgaaaccccg
     3001 cacatcgtgg tcgacgccgc ccaggagggc gtgcaggtgc cgtcgcagta cgtcaccgag
     3061 ggcaaggtgg tgctcaacat cggcggtaac gccacccagg gtctcagcct cggcaacgag
     3121 gcggtcgagt tcagcgcgcg cttcggcggc gtaccgtttc cggtgcgggt gccgctgcgc
     3181 gccgtgctgg gcatctacgc ccgcgagtcc ggcgagggca tgatcttcac cgactccgag
     3241 ggtgagccgc cgcccggcaa tgacggcggc gacggcgatt cgccgggacc gcagaagggc
     3301 ccgccgaagc tcagcgtcgt caaatagggg tcagaccccg gtttccagtt gtgcgcgggc
     3361 gcgcgggcct tcggtgccag tcgaccgcgg gtgccttcgg tttcagtcga ccgcgggcgc
     3421 gcggcgccat aggccggcct acgctcggac aaggaaaaac tcgctcggcc ggcctatg
//
LOCUS       DAOMPG010000243         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11241_length_3437_cov_5.109994, whole genome shotgun sequence.
ACCESSION   DAOMPG010000243 DAOMPG010000000
VERSION     DAOMPG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11241_length_3437_cov_5.109994"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLS34_11165"
     CDS             complement(<1..279)
                     /locus_tag="PLS34_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88061.1"
                     /translation="MKVDTTQSGRKARKFRRILWSVALVLGLSLALPLTGMLLLENPA
                     GQAVAQQGQAGDDAAAAVRREEQRREFWRQARGGAAGYSAVTGPETGVL"
     gene            complement(311..493)
                     /locus_tag="PLS34_11170"
     CDS             complement(311..493)
                     /locus_tag="PLS34_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88062.1"
                     /translation="MKNLTLALLAAGGLLLAGCSDVIFYEPGVYKGKSDPLVEDLRSE
                     ELRKELQERVNNSADR"
     gene            complement(550..1137)
                     /gene="fdh3B"
                     /locus_tag="PLS34_11175"
     CDS             complement(550..1137)
                     /gene="fdh3B"
                     /locus_tag="PLS34_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HRP88063.1"
                     /translation="MARMKFLCDSERCIECNGCVTACKNEHEVPWGVNRRRVVTLDDG
                     LPGERSISVACMHCTDAPCAAVCPVDCFYTTDDGIVLHDKDLCIGCGYCFYACPFGAP
                     QFPQKEAFGIRGKMDKCTFCAGGPESNNSEAELTKYGTNRIANGKLPLCAEMCATKAL
                     MAGDGDMVADIYRERVLKRGSRPQTWGWETAYKDA"
     gene            complement(1155..>3437)
                     /locus_tag="PLS34_11180"
     CDS             complement(1155..>3437)
                     /locus_tag="PLS34_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRP88064.1"
                     /translation="TVAGVANTWGYGAMTNSYNDMHKSKCMFFIGSNAAEAHPVAMQH
                     ILRAKENGSKMIVADPRFTRTAAHAHQHIRFRPGTDVALIWGMLWHVFENGWEDKEYI
                     SQRVLGMEDIRAEVAKWTPDVVENVTGVPGDHLKRAAQTMAENKPGTFVWCMGGTQHS
                     IGNANVRAYCVLQLALGNIGVSGGGANIFRGHDNVQGATDVGPNPDSLPGYYGIADAA
                     WTHWARVWDVDLNYLKSKFDFDKMYLEGAAETYRMNSPGIAVSRWIDGILEAKENVQQ
                     KDNIRMVFFQGHAVNSQVRGVEMKKALEKLDLVVISDPFPTHMAVMSDRKNDTYLLPT
                     CTQFEQAGSVTASNRSVQWREQVINPLFESKRDAEILYLFAQKFGFAEPMFKHIKVEN
                     GMPSPEDTLREINRGTFTIGYSGISPERLKLHLENKHTFNVTTLKAEGGPCDGETYGL
                     PWPCWGKPEHKHPGTHVLYDPSLPVKDGGLSFRVNFGLEYRGETLLAEGSWPVGSEIQ
                     DGYPAFTADMLKQLGWWDDLTDEEKKEAEGRSWTNDLSGGIIRVAIKHGCSPFGNGKA
                     RAIVWNFPDQVPLHREPLYSPRVDLVEQYPTHDDRKAQWRLPTRFISIQKAKDYSKEY
                     PLVFTSGRLTEYEGGGEETRSNPWLAELQQEMFVEINAADANDRGIKDGQWVWLEGAE
                     GGRIKIRAMVTPRVGRGVVWTPFHFGGWFQGKDLLDNYPEGTAPYVRGEACNTATTYG
                     YDPVTSMQETKTTLCQISAA"
BASE COUNT          553 a         1138 c         1066 g          680 t
ORIGIN      
        1 caggacgccg gtctctggcc ccgtgactgc cgagtacccg gcggccccgc cgcgggcctg
       61 gcgccagaac tcgcggcgct gctcctctcg ccggacggcg gcggctgcat cgtcaccagc
      121 ctggccctgc tgtgcaacag cctgtcccgc gggattctcc agcagcagca tgccggtcag
      181 cggcaatgcc agcgagagac cgagcaccag cgccacgctc catagaatcc tgcggaattt
      241 gcgcgccttc cggcccgatt gagttgtgtc gaccttcaca gtggattcct cccgtcttct
      301 tgaccgacgc ctagcgatca gccgaattat tgacgcgttc ctgcagttct ttcctcaact
      361 cttccgacct gaggtcttcc accagcgggt cgctcttgcc cttgtaaacg ccaggctcat
      421 agaaaatgac gtcggaacag cccgccaaca gcaggccgcc tgccgccagg agagccaggg
      481 tgagattttt cattgtattt ctccaaggtc gaaagcgggg cggccggtgc cgccccgccc
      541 cgagtacact tacgcgtcct tgtatgccgt ctcccagccc caggtctgcg ggcgactgcc
      601 acgcttgagc acgcgctcgc ggtagatgtc cgcaaccatg tccccgtcgc cggccatgag
      661 cgccttggtg gcgcacatct cggcgcacag cggcagcttg ccgttggcga tgcggttggt
      721 gccgtacttg gtgagttcgg cctcggagtt gttggactcc ggcccgcccg cgcagaaggt
      781 gcacttgtcc atcttcccgc gaatgccgaa ggcttccttc tgcgggaact gcggcgcccc
      841 gaacgggcag gcatagaagc agtagccgca gccgatgcac aggtccttgt cgtgcaggac
      901 gatgccgtcg tcggttgtgt agaagcagtc caccgggcag acggccgcgc acggggcgtc
      961 cgtgcagtgc atgcaggcca ccgagatcga gcgctcgccg ggcaggccgt cgtcgagggt
     1021 gactacccgc cgacgattca ccccccaagg aacttcgtgc tcgttcttgc aggcagtcac
     1081 gcagccgttg cactcgatgc aacgctcgga atcacacaaa aacttcattc ttgccatggt
     1141 gactttctcc tgcctcaggc cgcgctgatc tggcaaaggg tggtcttggt ttcctgcatg
     1201 ctggtgaccg ggtcataccc gtaagtggtc gccgtgttgc aggcctcgcc gcggacgtag
     1261 ggcgccgtgc cctccgggta gttgtcgagc aggtccttgc cctggaacca gccgccgaag
     1321 tggaatggcg tccacacgac gccgcgtccg acgcgcgggg tgaccatggc gcggatcttg
     1381 atcctgccgc cctcggcgcc ctcgagccaa acccactggc cgtccttgat gccgcggtca
     1441 ttcgcgtcgg ccgcgttgat ctcgacgaac atctcctgct gcagctcagc gagccacggg
     1501 ttggagcggg tttcctcacc gccgccctcg tactcggtca gtcggccact ggtgaatacc
     1561 agcggatact ccttggagta gtccttggcc ttctgaatgg agatgaagcg tgttggcagc
     1621 cgccactgcg ccttgcggtc gtcatgcgtc ggatactgct cgacgaggtc gacgcgcggc
     1681 gagtacagcg gctcgcggtg cagcggcacc tgatccggga agttccagac gatggcgcgc
     1741 gccttgccgt tgccaaacgg cgagcagccg tgcttgatcg cgacccggat gatgccgccg
     1801 gacagatcgt tcgtccagct gcgcccctcg gcctccttct tctcctcgtc ggtcaggtca
     1861 tcccaccagc cgagctgctt gagcatatcg gcggtgaagg ccgggtagcc gtcctggatc
     1921 tccgaaccga ccggccacga accttcggcc aagagggtct ccccacggta ctcgagaccg
     1981 aagttgacac ggaacgacag cccgccgtcc ttgaccggca agctggggtc gtacagcaca
     2041 tgggtgcctg gatgcttgtg ctccggcttg ccccagcacg gccacggcaa gccataggtc
     2101 tcgccgtcgc acggcccgcc ctcggccttc agggtcgtga cgttgaaggt gtgcttgttc
     2161 tccaggtgca gcttgagccg ttccgggctg atgccgctgt agccgatcgt aaaggtgccg
     2221 cggttgatct cgcgcagcgt atcctccggc gagggcatgc cgttctccac cttgatgtgc
     2281 ttgaacatcg gttcggcgaa accgaacttc tgcgcgaaca ggtagagaat ttctgcatcc
     2341 cgcttcgact cgaacagcgg gttgatgacc tgctcgcgcc actgcaccga acggttcgag
     2401 gccgtgacag aaccggcttg ctcgaactgc gtgcaggtcg gcagcaggta cgtgtcgttc
     2461 ttccggtcgc tcatgaccgc catgtgagtc gggaacgggt cggaaatgac caccaggtca
     2521 agcttttcga gcgctttctt catctcgacg ccgcgcacct ggctgttcac cgcatgcccc
     2581 tggaagaaca ccatgcggat gttgtccttc tgctgcacgt tttccttggc ctccaggatg
     2641 ccgtcgatcc agcgcgagac cgcgatgcct gggctgttca tgcgataggt ttcggccgcg
     2701 ccctcgagat acatcttgtc gaagtcgaac ttgctcttga gatagttgag gtcgacgtcc
     2761 cagacacgcg cccagtgcgt ccacgccgcg tcggcgatgc catagtagcc cggcagcgaa
     2821 tccgggttcg ggccgacgtc ggtcgcgccc tgcacgttgt catggccacg gaagatgttg
     2881 gcgccgccgc ccgacacgcc gatgttgccc agcgcgagct gcaggacgca gtacgcccgc
     2941 acgttggcgt tgccgatgct gtgctgggtg ccgcccatgc accacacgaa agtccccggc
     3001 ttgttctcag ccatggtctg ggccgcgcgc ttgagatggt cacccggaac gccggtaacg
     3061 ttctcgacca cgtcaggcgt ccacttggcc acctcagcgc ggatgtcctc catgccgagc
     3121 acgcgctggg aaatgtattc cttgtcctcc cagccgttct cgaatacatg ccacagcatg
     3181 ccccagatca gggccacgtc cgtgccggga cggaaccgga tgtgctggtg cgcatgggcc
     3241 gcggtgcgcg tgaagcgcgg gtcggccacg atcatcttgc tgccgttctc cttggcgcga
     3301 aggatgtgct gcatcgcgac cgggtgcgcc tcggcggcat tgctaccgat gaagaacatg
     3361 cacttggact tgtgcatgtc gttgtaggag ttggtcatcg cgccgtagcc ccaggtgttg
     3421 gctacaccgg ccacggt
//
LOCUS       DAOMPG010000244         3430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11268_length_3430_cov_4.429630, whole genome shotgun sequence.
ACCESSION   DAOMPG010000244 DAOMPG010000000
VERSION     DAOMPG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11268_length_3430_cov_4.429630"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="folD"
                     /locus_tag="PLS34_11185"
     CDS             <1..804
                     /gene="folD"
                     /locus_tag="PLS34_11185"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRP88065.1"
                     /translation="KVVVRTASGRSAPGLAVVLVGADPASAVYVRNKRRACERVGFAS
                     FDFDLPATTSEAELHVLIDRLNADPAVHGILIQLPLPAHIDTTALINRIDPAKDVDGF
                     HAANVGRLALRQPGLRPCTPRGILTLLAHTDRPVRGQEAVVIGVSNHVGRPMGLELLI
                     AGCTTTSCHRFTPRDTLRRHVGEADIVVVAVGRPDLVPGEWIKPGAIVIDVGINRRED
                     GSLCGDVGFDAAFERASWITPVPGGVGPMTVATLMQNTLDAAEMAWPVA"
     gene            918..2369
                     /gene="guaB"
                     /locus_tag="PLS34_11190"
                     /pseudo
     CDS             918..2369
                     /gene="guaB"
                     /locus_tag="PLS34_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002805758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            2509..>3430
                     /gene="guaA"
                     /locus_tag="PLS34_11195"
     CDS             2509..>3430
                     /gene="guaA"
                     /locus_tag="PLS34_11195"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRP88066.1"
                     /translation="MTSIHSDKLLILDFGAQYTQLIARRIREIGVYCEIWAWDHDPAE
                     IAAFGARGIILSGGPETTTVADSPRAPQEVFDVGVPILGICYGMQTMAVQLGGATEAA
                     DQREFGAAEVSLVAPDRLLDGLKDHAGSPPRLDVWMSHGDHVSRAPAGFVVTAKTDRI
                     PIAAFADDARRWYGVQFHPEVTHTRQGEALLRRFAVDICGCATRWNAEEIIEDQIARI
                     RAQVGSDEVILGLSGGVDSSVVAALLHRAIGGQLTCVFVDTGLLRWREGDQVMQTMAQ
                     HMGVKVIRVNAAARYFKALEGVSDPEAKRKI"
BASE COUNT          548 a         1194 c         1160 g          528 t
ORIGIN      
        1 aaggtggtgg tgcggacggc atcaggacgt tcggcccccg gcttggcggt agtgctggtg
       61 ggggccgatc cggcgtctgc ggtctacgtg cgcaacaagc gccgcgcctg cgagcgcgtc
      121 ggcttcgcct cgttcgactt cgacctgcct gccaccacct ccgaagccga gctgcacgtg
      181 ctgatcgatc gcctcaacgc cgatccggcc gtgcacggca tcctgatcca gctgccgctg
      241 ccggcgcata tcgataccac cgcgctcatc aaccgcatcg atcccgccaa ggacgtggac
      301 ggcttccacg ccgccaacgt cggtcgcctc gcgttgcgcc agcccggcct gcggccctgc
      361 actccgcgcg gcatcctcac gctgctggcg cataccgatc ggccggtgcg cgggcaggaa
      421 gcggtggtga tcggcgtatc caaccacgtc ggtcgcccga tgggtctgga gcttctgatc
      481 gccggctgca ccacaacgag ctgccaccgc ttcacgccgc gcgacaccct gcgccggcac
      541 gtcggcgagg ccgacatcgt ggtggtggcg gtcgggcggc cggacctggt gccgggcgag
      601 tggatcaagc ccggcgccat cgtcatcgac gtgggcatca accggcgcga ggacggctcg
      661 ctgtgcggcg acgtgggttt cgatgcggcc ttcgagcgcg cctcgtggat cacgcccgtc
      721 cccggcggcg tggggccgat gaccgtggcc acgctgatgc agaacaccct ggacgccgcg
      781 gaaatggcat ggccggtcgc ctgaccgcgg cgacccggtt ctgaacgtgc gtcatcccgg
      841 atcgccgtcg ggttcgtgaa tgccggccgt tctggcgtta caatggcgcg cttccccacc
      901 ttccacggga accgccgatg ctgcgcattc tggccgaagc actgacctac gacgacgttt
      961 cgctcgtccc cgcccattcc accgtcctgc ccaaggacgt agcgctcgaa acccggctga
     1021 cgcgcgacct gcgcctcagg attcccctgg tttccgccgc catggacacc gtgaccgaag
     1081 cgcgcctggc catcgccatg gcgcagctgg gcggcattgg catcatccac aagaatcttt
     1141 ccgccgagca gcaggccgcg gaagtgcgcc aagtgaagaa ttacgaggcc ggcgtgatcc
     1201 gcgagccctt caccgtcggc ccgcagacct ccatcggtga ggtgttgcgg ctcacccgcg
     1261 cgcgcaacat ctccggcgtg ccggtggtcg agggcggcaa gctcgtgggc atcgtcaccg
     1321 ggcgcgacat gcgcttcgag aagaagctcg acgatccggt gcgcaacatc atgacccgca
     1381 aggatcgcct cgtgacggtg cgtgaaggca ccagcgagga agaggtcatc cagctcctgc
     1441 acaagcaccg catcgagaag gtgctggtgg tcaatgacgc cttcgaactg cgcggcctga
     1501 tcaccgtcaa ggacatccag aaggcgcacg atttccccaa ctccgccaag gatgccgacg
     1561 agcagctcct gtgcggtgcg gccgtgggcg tgggtgccga cgccgaggcg cgcatcgagg
     1621 ccctggtcgc ggccggcgtg gacgtgctca tcgtcgacac cgcgcacggc cattcgcagg
     1681 gcgtgatcga gcgcgtggcc tgggtcaaga agacctatcc gcaggtgcag gtggtcggcg
     1741 gcaacatcgt caccggcgac gcggcgctcg ccctgatgga cgcgggtgcg gtgtaggtgg
     1801 gcgtcggccc aggctccatc tgcaccacgc gcgtggtcgc gggggtcggc gtgccgcagg
     1861 tgaccgcggt cagcctggtg gccgaggcgc tgaaggatcg cataccactc atcgccgacg
     1921 gtggcatccg ctactcgggc gacatcggca aggcgctggc ggccggagcc tcgaccatca
     1981 tgatcggcgg cctgttcgcc ggcaccgagg aagcgccggg tcaggtcgaa ttgttccagg
     2041 gccgcagcta caagagctac cgcggcatgg gcagtctggg cgcgatggag aagggcagca
     2101 aggaccgcta cttccaggac gccgccgacg ccgacaagct cgtgcccgaa ggcatcgagg
     2161 gccgcgtgcc ctaccgcggc ccggtcggcg gcatcgtgca ccaactcatc ggcggcctgc
     2221 gcgccaccat gggctatgtg ggttgtgcgt ccgtcgaaga gatgcgcacc cgcccgcagc
     2281 tggtgcgcat caccggtgcc ggccagcgcg aaagccacgt ccacgacgtc cagatcacca
     2341 aggaaccgcc gaactacagg atggggtgag gcgggccgct tgcgcggcac tgccgcgcgg
     2401 ccctgccgaa cgcccggcca tggatggccg ggcaggccgt ccgtgtggcg ctacgttccg
     2461 ccatggatgg ccgccgcaac ctgaaacgaa gaagcgatca ggctttccat gaccagcatc
     2521 cactccgaca agctcctcat cctcgacttc ggcgcgcagt acacccagct gatcgcgcgg
     2581 cgcatccgcg agatcggggt ctactgcgaa atctgggcct gggaccacga cccggcggaa
     2641 atcgcggcct tcggcgccag gggcatcatc ctctccggcg gcccggagac cacgaccgtg
     2701 gccgacagtc cgcgcgcgcc gcaggaagtt ttcgacgtcg gcgtgccgat tcttggaatc
     2761 tgctacggaa tgcagaccat ggccgtgcag ctgggcggtg ccaccgaggc cgccgaccag
     2821 cgcgagttcg gtgccgccga ggtcagtctg gtggcaccgg atcgcctgct cgatggcctc
     2881 aaggaccacg ccggttcgcc gccgcggctc gatgtgtgga tgagccatgg cgaccacgtc
     2941 tcgcgtgcgc cggccggttt cgtcgtcacc gcgaagaccg accgcattcc cattgccgcc
     3001 ttcgccgacg atgcgcggcg ctggtacggc gtgcagttcc atcccgaagt cacccacacc
     3061 cgccagggcg aggccctgct gcggcgcttt gcggtggata tctgcggctg cgccacgcgc
     3121 tggaacgccg aagagatcat cgaggaccag attgcccgca tccgcgccca ggtcggctcg
     3181 gacgaggtga tcctcggcct ttcgggtggc gtcgattcct ccgtggtggc cgcgctcctg
     3241 catcgcgcca tcggcggcca gctcacctgc gtgttcgtcg ataccggcct cctgcgctgg
     3301 cgcgaaggcg accaggtgat gcagaccatg gcgcagcaca tgggcgtcaa ggtgatccgc
     3361 gtcaacgccg ccgcgcgcta cttcaaggcg ctcgaaggcg tcagcgaccc ggaagccaag
     3421 cgcaagatca
//
LOCUS       DAOMPG010000245         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11302_length_3422_cov_3.270567, whole genome shotgun sequence.
ACCESSION   DAOMPG010000245 DAOMPG010000000
VERSION     DAOMPG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11302_length_3422_cov_3.270567"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..509
                     /gene="folK"
                     /locus_tag="PLS34_11200"
     CDS             9..509
                     /gene="folK"
                     /locus_tag="PLS34_11200"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRP88067.1"
                     /translation="MAVDALVPAYVGLGSNLDEPAVQLRRALTALGRLPGTRLVAVSS
                     FYGNPPMGPQDQPDFVNAVAGLLTTLPAMELLAALQAIEAAQGRRRDGPRWGPRTLDL
                     DLLVYGALRRDGEALVLPHPGIADRPFVVVPLHEIAPGLRLPGGPSIERLAAAADRGG
                     LARLDG"
     gene            509..1162
                     /locus_tag="PLS34_11205"
     CDS             509..1162
                     /locus_tag="PLS34_11205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRP88068.1"
                     /translation="MARALRYLAIEGPIGVGKTSLARRLAETLGFELVLERAEENPFL
                     ERFYRDPAANALPTQLHFLFQRARQAQEMRQADLFAGGRVADFLLEKDRIFARLTLPD
                     EEYRLYEQVYQGLALDAPTPDLVVYLQAPVETLIERVQRRGIAYEQAVRPDYLRRLSE
                     AYMDYFHRYDAGPLLIVNAAVIDPVRRDADFRDLLGRIQSMEGGRQYYNPAPFQPGD"
     gene            1247..2077
                     /gene="panB"
                     /locus_tag="PLS34_11210"
     CDS             1247..2077
                     /gene="panB"
                     /locus_tag="PLS34_11210"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRP88069.1"
                     /translation="MYSHLQDRARAEKPVTLDSLRKMKAAGEKIACLTAYDASFAAAV
                     DLAGTDLVLVGDSLGMVIQGHDTTVPVTMSDMVYHTRCVRRGVARAFLVADMPFGSYS
                     LPEPAFANAVRIMQEGGAAMVKLEGGALQVRTVEFLASRDIPVCAHIGLTPQSIHKLG
                     GFKVQGREERAAQQMLADAHALQEAGADIVLLECVPSVLGQAIAAALTVPVIGIGAGP
                     DVDGQILVLYDILSVTPGRKPRFAHDFLAGADGIADALARYVAAVRDGSYPAAEHCFS
                     "
     gene            2074..2928
                     /gene="panC"
                     /locus_tag="PLS34_11215"
     CDS             2074..2928
                     /gene="panC"
                     /locus_tag="PLS34_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRP88070.1"
                     /translation="MSGPPVVAAPAALRTQLDTWRREGLRIGFVPTMGNLHAGHLDLV
                     RAAAGRSNRVVVSIFVNPLQFGPGEDFAAYPRTLDEDLAKLAPEPCDLVFTPSEADMY
                     PRGRAGATRVEVPGLSDILCGAARPGHFSGVATVVARLFNLVQPELAAFGEKDRQQLA
                     VIRRMVEDLAFPVEILGVPTRREPDGLAMSSRNRYLGERERSVAPAMYQALRAAGEAL
                     ARGVDFVAIQQAGMDNIRSAGMAPEYFEIRRREGLEPAEPGDTALVLLAAARLGRARL
                     IDNLAVDL"
     gene            3009..3389
                     /locus_tag="PLS34_11220"
     CDS             3009..3389
                     /locus_tag="PLS34_11220"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRP88071.1"
                     /translation="MQLTLLKAKLHRACVTHAELDYEGSCAIDSDLLELAGIREYEQI
                     QIYNVTGGERFTTYAIRAEAGSRVISVNGAAAHKARPGDRVIICAYGVMSEQEARLHK
                     PKLVYLDEHNRVTRTGDAIPVQAA"
BASE COUNT          476 a         1201 c         1227 g          518 t
ORIGIN      
        1 ggttgatcat ggccgtggat gcgctggtgc cggcctatgt cgggctcggc agtaacctcg
       61 acgagccggc ggtgcagctg cggcgcgcgc tcacggcgct cgggcggctg cccggtacac
      121 ggctcgtggc cgtgtcgtcc ttctacggca atcctccgat ggggccgcag gatcagccgg
      181 atttcgtcaa cgcggtggcc gggctgctga ccacgctgcc ggccatggag ctgctggccg
      241 cattgcaggc gatcgaggcc gcgcagggcc ggcgccgcga cgggccgcgc tggggcccgc
      301 gcacgctgga cctcgacctg ctggtgtacg gcgcgctgcg gcgcgacggc gaagcgctgg
      361 tcctgccgca tccggggatt gcggatcggc ccttcgtggt ggtcccgctg cacgagatcg
      421 cgcccggcct gcggctgccg ggcggcccgt cgatcgagcg gctcgccgcg gcggcggacc
      481 gcggcggcct ggcgaggctc gacggctgat ggcccgcgcg ctgcgctacc tggccatcga
      541 ggggccgatc ggcgtcggca agacttcgct ggcgcgccgg ctggccgaga cgctgggctt
      601 cgaactggtg ctcgagcgcg ccgaagagaa cccgttcctc gagcgcttct atcgcgaccc
      661 cgccgcgaac gccctgccga cccagctgca cttcctgttc cagcgggcgc gccaggcgca
      721 ggaaatgcgc caggccgacc tgttcgccgg cggccgcgtg gccgattttt tgctcgagaa
      781 ggaccgcatc ttcgcgcgcc tcacgctgcc ggacgaggaa taccgcctct acgagcaggt
      841 ctaccagggc ctggcgctgg acgcgcccac gcccgacctc gtggtctacc tgcaggcgcc
      901 cgtcgagacg ctgatcgagc gcgtccagcg ccgcggcatc gcctatgagc aggcggtacg
      961 gccggactac ctgcggcgcc tctccgaggc ctacatggac tacttccacc gctacgacgc
     1021 gggtccgctg ctgatcgtca acgcggccgt catcgacccg gtgcgccgcg acgccgattt
     1081 ccgggacctg ctgggccgca tccagtcgat ggagggcggg cgccagtact acaatccagc
     1141 gcccttccag ccgggcgatt gagccgggga gcggcgccgg attgatgttt cccggtgagt
     1201 tggccatgct ggctcgcctt gccgcgcggg gaattgccgc atgaccatgt acagccacct
     1261 gcaggaccgg gcccgcgccg aaaagccggt gaccctggac agcctgcgca agatgaaggc
     1321 cgccggcgag aagatcgcct gcctgaccgc ctacgacgcc agcttcgcgg cggcggtgga
     1381 cctggccggc acggacctgg tgctggtagg cgactcgctc ggcatggtga tccagggcca
     1441 tgacacgacc gtgccggtga ccatgagcga catggtgtat cacacgcgct gcgtgcgccg
     1501 cggcgtggcc cgcgccttcc tggtggccga catgccgttc ggcagctact cattgcccga
     1561 gcccgctttc gccaacgcgg tgcgcatcat gcaggagggc ggtgcggcga tggtaaagct
     1621 cgaaggcggc gcgttgcagg tgcgcaccgt cgagttcctc gcctcgcgcg acatcccggt
     1681 gtgcgcccac atcggcctga cgccgcagtc catccacaag ctcggcggct tcaaggtgca
     1741 gggccgcgaa gagcgggccg cacaacagat gctggccgac gcccacgcgc tgcaggaggc
     1801 cggcgcggac atcgtgttgc tggaatgcgt gccctcggtg ctcggccagg cgattgccgc
     1861 ggcgctcacc gtgccggtca tcggcatcgg cgcggggccg gacgtggacg gccagatcct
     1921 ggtgctgtac gacatcctct ccgtgacgcc cggccgcaag ccgcgcttcg cgcatgactt
     1981 cctcgccggc gccgacggca ttgccgacgc gctggcgcgc tacgtcgcgg cggtgcgcga
     2041 cggcagctac ccggccgcgg agcactgctt ctcgtgagcg gcccgccggt ggtcgcggcg
     2101 cctgcggcgc tgcgcacgca actcgacacc tggcgccgcg agggcttgcg gatcggcttc
     2161 gtgccgacca tgggcaacct gcatgccggc cacctggacc tggtgcgcgc ggcggccggt
     2221 cggtccaacc gggtggtggt gagcattttc gtgaacccgc tgcagttcgg ccccggcgag
     2281 gacttcgccg cctatccgcg caccctggac gaagacctgg cgaagcttgc gcctgagcct
     2341 tgcgacctcg tgttcacgcc gagcgaggcg gacatgtacc cgcgcggccg ggcgggcgcg
     2401 acgcgggtcg aggtgccggg cctgtctgac atcctctgcg gcgctgcccg gccggggcat
     2461 ttttccggcg tggcgacggt cgtggcgcgg ctgttcaacc tggtgcagcc tgagctcgcc
     2521 gccttcggcg agaaggaccg ccagcagctc gcggtcatcc ggcgcatggt cgaggacctg
     2581 gcgttcccgg tggagatcct cggcgtgccg acgcgccgcg agccggacgg cctggcgatg
     2641 agctcgcgca accgctacct gggcgagcgg gagcggtcgg tcgccccggc catgtaccag
     2701 gcgctgcgcg cggcagggga ggcgctggcc cgcggggtcg attttgttgc aatacagcag
     2761 gcgggcatgg acaatatccg gtcggcaggc atggcgccgg agtacttcga gatccgccgc
     2821 cgggaggggc tggagcccgc ggaacccggc gatacggcgc tggtcttact cgctgcggcc
     2881 cggctgggcc gggcgcggtt gattgacaac ctcgccgtgg acctatagtt aaccgactcc
     2941 cggcgcggcc cgccggggcg accggcaccg aaagcaacgc gcgacgaggg cgtgggaagg
     3001 aggaaccgat gcagctgact ttgctcaagg ccaagctcca ccgggcctgc gtgactcacg
     3061 cagagctgga ctacgagggc tcgtgcgcga tcgattccga cctgctggag ctggccggca
     3121 tccgcgagta cgagcagatc cagatctaca acgtcaccgg cggtgagcgt ttcaccacct
     3181 atgccatccg cgccgaggcc ggctcgcggg tgatctccgt gaacggcgcc gcggcccaca
     3241 aggcgcgccc cggcgaccgt gtgatcattt gcgcctacgg cgtcatgtcc gagcaggaag
     3301 cgcgcctgca caagccgaag ctcgtgtatc tcgacgagca caatcgcgtc acgcgcaccg
     3361 gcgacgccat ccccgtccag gccgcctgag gccgattctc agctctcgac ccggttccgc
     3421 cc
//
LOCUS       DAOMPG010000246         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11394_length_3401_cov_4.014943, whole genome shotgun sequence.
ACCESSION   DAOMPG010000246 DAOMPG010000000
VERSION     DAOMPG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11394_length_3401_cov_4.014943"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1393)
                     /locus_tag="PLS34_11225"
     CDS             complement(71..1393)
                     /locus_tag="PLS34_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP88072.1"
                     /translation="MTLSRFASRMSAVGFGLVLSACGGSGGSSDITGNCSIPAQNQAV
                     YETMQQWYLWYEKLPVLNPAGFASPQALLDALVSQVADPVDRFSYLTTQAEEDALFGE
                     SQFIGFGFRQQTGTDSVTVLDVFEDGPADLAGLDRGSSILAIDGVPIADILASAEGLG
                     GALGPAEIGYEIEMTFRNQAGAELTSTLVKDTVTIPPVTATQVFDLHGVTTGYLVFRN
                     FVDPGIPALNAAFTEFSNAGVTQLIVDLRYNGGGLLRVMEHFANLLASRDHPGLPFYS
                     LWHNDKNSGRNETFLFTTNPSPVALSLDKLVLITTSATASASEMLVNGLPPYIETITV
                     GSETFGKPVGQYGFRFCEQVLRPVSFEVVNALGEGDYFEGIAPDCAAADNVAVPFGVA
                     GEDSFDMAVNWLWTGSCALPARPQPMERLAAEPAEPAARPRYRPNDAW"
     gene            complement(1390..1896)
                     /gene="folK"
                     /locus_tag="PLS34_11230"
     CDS             complement(1390..1896)
                     /gene="folK"
                     /locus_tag="PLS34_11230"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRP88073.1"
                     /translation="MPEVFVGAGSNVEPRRHLGAGLRGLAEQFGLLRLSPVYRNSPVG
                     FAGEDFLNLVIAFATDDPVEEVAAALAAIEAANGRVRGAAKFAPRTLDLDLLLYGDLV
                     IDADGIQLPRDEITRYSFVLKPLADLAGERLHPVLGRSFAELWEHYDGERHPLERVPG
                     DFAAEVAL"
     gene            complement(1889..2254)
                     /gene="folB"
                     /locus_tag="PLS34_11235"
     CDS             complement(1889..2254)
                     /gene="folB"
                     /locus_tag="PLS34_11235"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRP88074.1"
                     /translation="MDTIFLNDLRVETVIGIYEWERRIRQTVSINLEIAADIRRAAAS
                     DSIDDTLNYKAVAKRLIEFVEGADFQLVETLAERIAGIVCTEFDVSRVKVTLNKPGAI
                     RGARDVGVCIERTSADYHA"
     gene            complement(2263..3315)
                     /gene="tsaD"
                     /locus_tag="PLS34_11240"
     CDS             complement(2263..3315)
                     /gene="tsaD"
                     /locus_tag="PLS34_11240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRP88075.1"
                     /translation="MRVLGIETSCDETGVAVFDAERGLLAHALYSQVKLHAEFGGVVP
                     ELASRDHVRKLLPLIKQVLKQSGTAPEALDGVAYTAGPGLIGALLVGACVGRSLAWAW
                     GVPAVGVHHMEGHLLAPMLEDPAPEFPFIALLVSGGHSMLVEVAGLGRYRVLGETLDD
                     AAGEAFDKTAKVLGLGYPGGPQLARLAERGAPGRLQLPRPMLNRPGLDFSFSGLKTAV
                     ITGIRDLELDEQARADVALEFQEAIVDTLVGKSLRAVEQTGISRLVVAGGVGANRRLR
                     ERLAAAAQQAGAEVFYPRLEFCTDNGAMIALAGMLRLAAGEAAPAAIEARARWDLETL
                     QPPASGTAQPGPAPAN"
BASE COUNT          507 a         1171 c         1206 g          517 t
ORIGIN      
        1 cggctggacg tcgctcgtcg actgggtgcg gatcgtcgcc tgcgcggtgc cggccgcccg
       61 ccggtgttcg ctaccaggcg tcgttcggcc ggtagcgcgg ccgcgcggcg ggctcggccg
      121 gctcggcggc caagcgctcc atgggctggg gtcgggccgg caacgcgcaa ctcccggtcc
      181 acagccagtt caccgccata tcgaaggaat cctcaccggc cacaccgaac ggcaccgcga
      241 cattgtcggc ggcggcgcag tccggcgcga tgccctcgaa gtagtcccct tcaccgagcg
      301 cgttgaccac ctcgaaggac accggccgca acacctgctc gcagaagcgg aagccgtact
      361 ggccgaccgg cttgccgaag gtctcgctgc ccaccgtgat ggtctcgatg tagggcggca
      421 ggccgttcac cagcatctcc gacgccgagg ccgtggcgga cgtggtgatg agcaccagct
      481 tgtcgagcga cagcgcgacc ggcgacggat tggtggtgaa caggaaggtc tcgttgcggc
      541 cgctgttctt gtcgttgtgc cagaggctgt agaacggcag gcccgggtgg tcgcggctgg
      601 ccagcaggtt ggcgaagtgc tccatgacgc gcagcagccc gccgccgttg tagcgcaggt
      661 ccacgatcag ctgggtgacg ccggcattgc tgaactcggt gaaggccgcg ttcagcgccg
      721 ggatgccggg atcgacgaag ttgcggaaca ccaggtagcc ggtggtgacg ccgtggaggt
      781 cgaagacctg ggtcgcggtc accggcggga tggtcacggt gtccttgacc agcgtggacg
      841 tgagctcggc gccggcctgg ttgcggaagg tcatctcgat ttcgtagccg atctcggccg
      901 ggcccagcgc gccgccgagc ccttcggcgg aggccaggat gtcggcgatc ggcacgccgt
      961 cgatggccag gatgctgctg ccgcgatcca gcccggcgag gtcggccggc ccgtcctcga
     1021 acacgtcgag cacggtgacc gagtcggtgc cggtctgctg gcggaagccg aagccgatga
     1081 actggctctc gccgaacagg gcgtcctcct cggcctgggt ggtgaggtag ctgaagcggt
     1141 cgaccggatc tgcgacctgc gagaccagcg cgtcgagcag cgcctgcggc gaggcgaagc
     1201 cggccgggtt gagcaccggc agcttctcgt accacaggta ccactgctgc atggtctcgt
     1261 agacggcctg gttctgcgcc ggaatcgagc aattgccggt gatgtcgctg gagccgccgg
     1321 agccgccgca ggcggacaac accaggccga agcccaccgc tgacatgcgg ctcgcgaatc
     1381 gggacagggt cataacgcta cctccgcggc gaagtcgccc ggcacccttt caagggggtg
     1441 gcgctcgccg tcatagtgtt cccagagctc ggcaaagctt cgaccgagca cgggatgcag
     1501 acgttccccg gcgaggtccg ccaggggctt caagacgaag gaataccggg tgatctcgtc
     1561 gcgcggcagc tggatgccgt cggcgtcgat gaccaggtcg ccgtacagca gcaggtcgag
     1621 atcgagcgtg cgcggcgcga acttggccgc gccgcggacc cggccgttgg ccgcctcgat
     1681 ggccgccagc gccgcggcga cctcttcgac cgggtcgtcg gtcgcgaagg cgatcaccag
     1741 gttgaggaaa tcctcgcccg caaagcccac cgggctgttc cggtagaccg gcgagaggcg
     1801 cagcagcccg aactgctccg ccagcccgcg cagccccgca ccgagatggc ggcggggttc
     1861 gacgttgctg ccggcgccga cgaagacttc aggcatggta gtccgcggag gtgcgctcga
     1921 tgcacacccc gacgtcgcgc gcgccgcgaa tggcgcccgg cttgttgagc gtgaccttga
     1981 cgcggctcac gtcgaactcg gtgcagacga tgccggcgat gcgctcggcc agcgtctcca
     2041 ccagctggaa gtcggcgccc tcgacgaact cgatcagccg cttcgccacg gccttgtagt
     2101 tgagcgtgtc gtcgatgctg tcggaggccg ccgcgcggcg aatgtcggcg gcgatctcga
     2161 ggttgatgct gacggtctgg cggatgcgcc gctcccactc gtagatgccg atgacggttt
     2221 ccacgcgcag gtcgttgaga aatatcgtgt ccactcggat cctcagttgg ccggcgccgg
     2281 gccgggctgg gcggtgccgc tcgccggcgg ctgcagggtt tcaaggtccc accgggcgcg
     2341 cgcctcgatg gcggccggcg cggcctcgcc ggcggccagc cgcaacatgc cggccagggc
     2401 aatcatcgca ccattatcgg tgcagaactc cagccgcggg tagaacacct cggcgccggc
     2461 ctgctgcgcc gccgccgcaa ggcgctcgcg caggcggcga ttggcgccca cgccgcccgc
     2521 caccaccagc cgggagatgc cggtctgctc gaccgcacgc aggctcttgc cgaccagcgt
     2581 gtcgacgatc gcctcctgga attccagcgc cacgtccgcc ctcgcctgtt cgtcgagctc
     2641 caggtcgcgg atgccggtga tcaccgcggt cttcaggccg ctgaagctga aatccagccc
     2701 gggccggttg agcatggggc gcggcaactg cagccgcccg ggggcgccgc gctcggcgag
     2761 ccgcgcaagc tgcggcccgc ccgggtagcc gaggccgagc accttggcgg tcttgtcgaa
     2821 ggcctcgccg gcggcgtcgt cgagggtctc gcccagcacc cggtagcggc ccaggccggc
     2881 gacctccacc agcatgctgt ggccgccgga caccagcagc gcgatgaaag ggaattccgg
     2941 cgccgggtcc tcgagcatcg gcgccagcag gtggccctcc atgtggtgca cgccgacggc
     3001 cggcacaccc caggcccacg ccaggctgcg gccgacgcag gcccccacca gcagtgcccc
     3061 gatcaggccg ggaccggccg tgtaagccac cccgtcgagt gcctccggtg ccgtgccgga
     3121 ctgcttaagg acttgtttta tcagcggaag cagcttgcgg acgtggtcgc gggaggccag
     3181 ttccggcacc acgccgccga actcggcgtg cagcttcacc tggctgtaga gggcgtgcgc
     3241 cagcaggccg cgctcggcgt cgaacacggc caccccggtc tcgtcacagg aggtctcgat
     3301 gccgagcact cgcattgtct tgttcctcgt ggctgttggc cctttgccta ctggctttcg
     3361 ccccgcgggg ccgctagaat ggtcggtctt tacccggtcg g
//
LOCUS       DAOMPG010000247         3377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11481_length_3377_cov_4.444311, whole genome shotgun sequence.
ACCESSION   DAOMPG010000247 DAOMPG010000000
VERSION     DAOMPG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11481_length_3377_cov_4.444311"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="PLS34_11245"
     CDS             complement(<1..1793)
                     /locus_tag="PLS34_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP88076.1"
                     /translation="MSILAELKRRNVLRIAAAYLVAAWLVIQVVETITPSFGFGDTAV
                     RVVTLVAAIGLVPVLVLAWVFELTPDGIRKDSEVDRDAPRLARAARRLDRIILVVLVL
                     AVAYFAVDKFLLAEWLTAGPEGRGGAATEHAAGHDTAPDETAVDRSIAVLAFQDLSQG
                     HDHEYLSDGIAEELLNRLARIPELRVTSRYSAFYYKGKDIRLADIARELNVGYILAGS
                     LRTSGDQVRVTAQLVHAESDVQVWSESFDRSMGDIFVIQDEIAAAVATQLRARLLGSA
                     PRARQPDPQAYSLVLQARHVIRRATAEAYEEAIPLLAEAAAIDPGYAVPWDSLSLIYS
                     TQAGRGLRPSEEGYGLAAEMARKALEIDPEHAPAHARLGWIEMTHHKDLGAAAGHFER
                     ALALDPGDIGIIANAAVLLQTLGRIEQATVLLEHLTLRDPVWTGGHYNLGYYYLAAAR
                     WDDAIDSYRTALRLSPGRIGANNFIGIALLKKGDAQAARQAFEQEPHEVLRRLGLVMA
                     DHALGRNAEADAGMAELIAEHERDWAYYIAAVFAFRDNIEAAFKWLDKAVEYNSSGLQ
                     GILVNPLLRSLHADPRWLPLLESMGKSPRQL"
     gene            complement(1853..2833)
                     /gene="pbpG"
                     /locus_tag="PLS34_11250"
     CDS             complement(1853..2833)
                     /gene="pbpG"
                     /locus_tag="PLS34_11250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HRP88077.1"
                     /translation="MSSVASSGPRANIRYRTGVLATAGRNRLVSRAVICAAALAWALV
                     AAPAAAQPPLDPAGLELASTQAAVVDLASGELLFAKHADRQVPIASVTKLMSAVVVLD
                     SGAPLDQWLTIVRRAEPAPANAYSRIRIGSELRRADLLRITLMASENQAAYVLSRHHP
                     GGREAFVAAMNDKAIELGMHATHFVDASGLSAENRSTAADLLRLVAAAMEYELIREYT
                     RTTGFTAQFRNPRYTLQYGNTNLLVHRESWDVALSKTGYLQIAGRCLAMVANIDGRPI
                     AMVLLDSLGTRTPLGDAGRIGRWLRTGQGGSVAAAARDYERQRQREYARD"
     gene            complement(2964..>3377)
                     /locus_tag="PLS34_11255"
     CDS             complement(2964..>3377)
                     /locus_tag="PLS34_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRP88078.1"
                     /translation="LAEWTIRMAERRAFGIFNAPGPDYELSMEAMLYGIRAVTTNGAR
                     LTWVPAEFLGRHDVRPWADMPTWLPGTGDTAGFSRVSNARAVAAGLTFRALATTAADT
                     LEWFRSEPAERRAELRAGLSAEREKAVLSAWHARG"
BASE COUNT          545 a         1158 c         1125 g          549 t
ORIGIN      
        1 tcgagctgcc gcggcgactt gcccatcgac tccagcaagg gcaaccagcg cggatccgca
       61 tgcaggctgc gcaacaaggg attgacgagg atgccctgca gccccgagct gttgtattcg
      121 acggccttgt ctaaccactt gaaggcagcc tcgatgttat cccggaaggc aaaaaccgcg
      181 gcgatgtaat acgcccaatc gcgctcgtgc tcggcgatca gctccgccat cccggcatcg
      241 gcctcggcgt tgcggccgag tgcgtggtcg gccatcacga gtcccagtcg ccgcaacacc
      301 tcatggggtt cctgctcgaa tgcctgcctg gccgcctgcg cgtcgccttt cttcaacaac
      361 gcgatgccga tgaaattgtt ggcaccgatc ctccccggac tcagccgcaa cgcggtccgg
      421 tacgagtcga tggcgtcgtc ccaccgtgcg gcggcgaggt agtagtagcc caggttgtag
      481 tggccgccgg tccagacggg atcacgcagc gtgaggtgct cgagcagcac ggtcgcctgc
      541 tcgatcctgc ccagcgtctg cagcaggacg gccgcgttgg cgatgatgcc gatgtcgccc
      601 ggatccagcg ccagcgcacg ctcgaagtgc ccggccgcgg cgccgaggtc cttgtggtgg
      661 gtcatttcga tccagccgag ccgcgcgtgg gcgggcgcat gttccgggtc gatttccaac
      721 gccttccggg ccatctcggc agcgagcccg tagccctcct ccgagggccg caggcccctg
      781 cccgcctgtg tcgagtagat cagcgagagt gaatcccagg gcaccgcgta gcccggatct
      841 atcgcggccg cttctgccag cagcggaata gcttcctcgt aggcctcggc ggtggcgcgc
      901 cggatgacgt gacgcgcctg cagcacgagg ctgtaggctt gcgggtcggg ttggcgggct
      961 cgcggcgcac tgcccaacag ccgggcgcgc aactgggtcg ccacggccgc ggcgatctcg
     1021 tcctggatga cgaagatgtc gcccatgctg cgatcgaagc tctcggacca gacctggacg
     1081 tcggattcgg catgcacgag ctgggccgtg acccgcacct ggtcgcccga ggtgcgcagc
     1141 gatccggcga gaatgtagcc gacgttgagc tcgcgcgcga tatccgccag gcggatgtcc
     1201 ttgcccttgt agtagaacgc cgaatagcgc gaggtgacgc gcagctccgg aatccgggcc
     1261 agccggttca gcagctcctc cgcgattccg tcggacaggt actcgtggtc gtgcccctgg
     1321 gacagatcct ggaaggccag caccgcaatc gagcgatcca cggcggtctc gtcgggcgcg
     1381 gtgtcgtgcc ccgccgcgtg ctccgtggct gccccgcccc gcccctccgg gcctgccgtc
     1441 agccactccg ccaggagaaa cttgtcgacg gcaaagtagg ccacggccag gaccagcacc
     1501 acgagaatga tgcgatcgag ccgcctggcc gcccgggcca ggcgcggcgc atcccggtcg
     1561 acttctgagt ccttcctgat gccgtccggc gtgagttcga acacccacgc cagcacgagc
     1621 accggcacga gcccgatggc ggcgaccagg gtcacgaccc gcaccgccgt gtccccgaag
     1681 ccgaacgagg gcgtaatggt ttccacgacc tggatgacca gccaggcggc caccaggtaa
     1741 gcggcggcga tgcgcagcac gttgcggcgc ttgagttcgg ccaggatgga catgggtcag
     1801 tctcccggaa ttcgccggat gatgcggcag ccggctcaac ttgtcgaccg cgtcagtcgc
     1861 gggcgtattc gcgctgtcgc tgacgctcat agtcgcgggc cgcggctgca acactgccgc
     1921 cctgccccgt gcgcagccag cgaccgatgc gcccggcgtc ccccagcggc gtgcgcgtgc
     1981 cgagcgaatc gagcagcacc atcgcgatgg gtcggccgtc gatgttggcc accatggcga
     2041 ggcagcgccc ggctatctgc aggtagccgg tcttgctcag tgcgacgtcc cagctctcgc
     2101 gatgcaccag caggttggtg ttgccgtact gcagcgtgta gcgcggattg cggaactgcg
     2161 ccgtgaagcc cgtggtgcgc gtgtattccc tgatgagctc gtattccatt gctgccgcga
     2221 ccagccgcag cagatctgcg gcggtggagc gattctctgc ggacaggcca ctggcatcca
     2281 cgaagtgggt ggcgtgcata ccgagttcga tcgccttgtc gttcatcgcg gccacaaagg
     2341 cttcgcgccc gcccggatga tgacgcgaga ggacataggc ggcctggttc tccgaggcca
     2401 tcagcgtgat gcgcagaaga tcggcgcgcc gcagctccga gccgatgcga atacgggagt
     2461 aggcgttcgc cggggcaggc tcggcgcgcc gcacgatcgt cagccactga tccagcggcg
     2521 cgccagagtc gagtacgacc acagcgctca tcagcttggt caccgacgcg atcggcacct
     2581 gccggtcggc atgcttggcg aacagcagct cgccgctggc aaggtcgacc actgccgcct
     2641 gcgtggaggc cagctccaga cccgcaggat ccaggggtgg ctgcgctgcc gctggtgctg
     2701 ccaccaaggc ccaggccagt gcggcggcac agatgacggc ccggcttacc agccggttgc
     2761 ggcctgccgt ggcgaggacg cctgtccgat agcggatgtt cgctcgaggt ccgcttgatg
     2821 ccacgctgct catggcctcc gagaataccg tatcgcgcgg taccatagcg cctcgtcgcc
     2881 ggcattggcg tgctgcagct gttctccaac tccgtcaggt gaagaagcag gaagatgtcg
     2941 tggcagtatg cgtcagctga ggatcagccc cgcgcgtgcc acgccgacag cacggctttt
     3001 tcgcgctcgg cggacaatcc ggcgcgaagc tcggcccgac gctcggccgg ctcgctccgg
     3061 aaccactcca aggtgtcggc ggcggtcgtc gccaacgcgc ggaacgtcag tcccgcagca
     3121 accgccctgg cgttggagac ccgtgaaaag cccgctgtgt cgccggtgcc cggcagccac
     3181 gtcggcatgt ccgcccaggg cctgacgtca tgacgcccca ggaactcggc gggtacccag
     3241 gtcaagcggg ccccattcgt cgtaactgcc cggatcccgt aaagcattgc ctccatgctc
     3301 agctcgtaat ccgggcccgg cgcattgaat atcccgaagg cgcgccgctc ggccatccgg
     3361 atggtccact cggcgag
//
LOCUS       DAOMPG010000248         3370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11514_length_3370_cov_5.095626, whole genome shotgun sequence.
ACCESSION   DAOMPG010000248 DAOMPG010000000
VERSION     DAOMPG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11514_length_3370_cov_5.095626"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..2561)
                     /locus_tag="PLS34_11260"
     CDS             complement(6..2561)
                     /locus_tag="PLS34_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP88079.1"
                     /translation="MGIFRRLERSMVASYDRTVIRHPVLTLLAAALLVAVLGAFAPRF
                     QLDASSETLVMEHDEAVRYYRIIRARYESDDFLVVTYTPHAGLFTERALARLEQLRDA
                     LLAVDEVESVTTLLDVPLIDGLGVSLTDLPTDPEQLNCNGPDRVQDCRRALDSDLYRN
                     LLVSPDGATTGIQVNLRQDEAFRALQRERDQLRIQRQSEELDEAGQRRIDELDRQIAT
                     QGAVVREREATAIAQVRGVLDGFRDDARIHLGGVPMIAVDSIAFIRADLVNFGAAVGA
                     FIVLILFLAFRRWYWVVLPVITCAATVVAMVGLLGLLGWPVTVVSSNFISLLLILNLA
                     LVVHLIVRYRELHELHPERDHAGLVRDTVLSKFTPCLYTALTTMVAFGSLLVSGIRPV
                     IDFGWMMVIGLGVAFLLSFTLFPAALMLIRTGRPVALRNVTGAITGALARAITARPGT
                     TLLVSLLLAVAGVAGTFQLSIENRFIDYFKKSTEIYQGMRLIDEQLGGTTPLDVIIDA
                     PAAGPEDEAGEDDDFVDEFEPLDDFDTGMDAGIASTSYWLNSTRLPEVRAIHAYLESL
                     PGTGKVLSIATAMDMFGALEPRVLNEDFFLSVFYRRLPEAVKAALFDPYLSEDGQQLR
                     FSIRVYESAPDLRRNEMLEEIRRHIAEEMGYGAGQVQLSGMMVLYNNMLQGLFRSQIL
                     TLGVVFGAIMLMFLVLFRSWRFSALGILPNLLSAAMVLGLMGWVRIPLDLMTVTIAAI
                     TIGIGVDNTIHYVHRYREELAVDGNPWEAVARCHGSIGRALYYTSVTIVLGFSVLALS
                     AFIPTILFGLLTGLAMTAALLANLTLLPVLLAKTEKAQNRGQEKPGSETGFAR"
     gene            complement(2589..>3370)
                     /locus_tag="PLS34_11265"
     CDS             complement(2589..>3370)
                     /locus_tag="PLS34_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HRP88080.1"
                     /translation="GLAAVFYPRRATDLPPVPVDLPEPPAASDPADGPAAPPAEPEPA
                     PAFVAEEAVEPATPLPSLDESDEAARAALAEVAGETLVERYLVSDALVRKLVATVDNL
                     PREALWIKARAVPPIEGRFIAEGPEDGLVIGPANHARYAPLVRLVGALDAVALAAAYQ
                     RHYPLLQQAYEELGYPGRQFHNRALEVIDHLLATPRATGPIRLAQPHVLYTFADPSLE
                     ALSPGQKVLLRIGPDNAGIIRAKLIELRAALEDLSAAVPAN"
BASE COUNT          572 a         1137 c         1173 g          488 t
ORIGIN      
        1 caggtttagc gagcgaaacc tgtctctgac cccggttttt cctgaccccg gttttgcgcc
       61 ttctcggtct tcgccagcag caccggcagc agcgtcaggt tggccagcag cgcggcggtc
      121 atcgccagcc cggtcagcag gccgaacagg atcgtcggga tgaaggccga cagggccagc
      181 accgagaaac cgagcacgat ggtcacgctg gtgtagtaca gcgcgcggcc gatcgaaccg
      241 tggcaacgcg ccacggcctc ccaggggttg ccgtcgacgg ccagctcttc gcgatagcga
      301 tggacgtagt ggatggtgtt gtcgacgccg atgccgatgg tgatcgcggc gatggtcacg
      361 gtcatcaggt ccagcgggat tcgcacccag cccatcagcc ccagcaccat ggccgccgac
      421 agcaggttcg gcaggatgcc cagcgccgag aagcgccagg agcggaacag caccaggaac
      481 atcagcatga tggcgccgaa cacgaccccc agcgtgagaa tctgcgaacg gaacaggccc
      541 tgcagcatgt tgttgtacag caccatcatg ccggacagct gcacctgccc ggcgccgtag
      601 cccatctcct ccgcgatgtg ccggcggatc tcctcgagca tctcgttgcg gcgcaggtcc
      661 ggcgccgatt cgtacacgcg gatgctgaag cgcaactgct gcccgtcctc cgacaggtag
      721 gggtcgaaca gcgccgcctt gacggcttcc ggcaggcgcc gatagaacac ggacaggaag
      781 aaatcctcgt tcagcacgcg cggctcgagc gcgccgaaca tgtccatggc ggtggcgatc
      841 gacaacacct tgccggtccc tggcagcgac tcgaggtacg cgtggatggc gcgcacctcg
      901 ggcaggcgcg tactgttgag ccagtagctg gtgctggcga tgccggcgtc catgccggtg
      961 tcgaagtcgt ccagcggctc gaattcgtcg acgaaatcat cgtcctcgcc cgcctcgtcc
     1021 tccgggccgg cggcgggcgc atcgatgatc acgtccagcg gggtggtacc gccgagctgc
     1081 tcgtcgatca gccgcatgcc ctggtagatc tcggtgctct tcttgaagta gtcgatgaag
     1141 cgattctcga tcgacagctg gaaagtgccg gccacgccgg ccaccgccag cagcagtgac
     1201 accagcagcg tggtgccggg gcgcgcggtg atcgcccggg cgagcgcgcc ggtgatcgcc
     1261 cctgtgacgt tgcgcagcgc caccgggcgc ccggtcctga tcagcatcag cgcggcagga
     1321 aacagcgtga aagacagcag gaaggccacg cccagcccga tcaccatcat ccagccgaaa
     1381 tcgatgaccg gccggatgcc gctgaccagc agggagccga acgcgaccat ggtggtgagg
     1441 gcggtgtaca ggcagggcgt gaacttgctg agcaccgtgt cccgcaccag gcccgcatgg
     1501 tcccgctcgg gatgcagctc atgcagttcc cggtagcgca cgatcaggtg caccaccagc
     1561 gccaggttca ggatcagcag cagcgagatg aagttcgacg acacgaccgt gaccggccag
     1621 cccagcagcc cgagcagccc gaccatcgcc accaccgtcg ctgcgcaggt gatcaccggc
     1681 aacaccaccc agtaccagcg gcggaacgcc aggaacagga tcagcacgat gaaggcgccg
     1741 accgcagcgc cgaaattgac caggtcggcg cggatgaagg cgatcgagtc caccgcgatc
     1801 atcggcacgc cgccgaggtg aatccgggca tcgtcccgga agccgtcgag cacgccgcgg
     1861 acctgcgcga tcgccgtggc ctcccgctcg cgcaccaccg cgccctgtgt cgcaatctgc
     1921 cgatccagct cgtcgatccg ccgctgcccg gcttcgtcaa gttcttcgga ctggcgctgg
     1981 atgcgcagct ggtcgcgctc gcgctgcagg gcgcggaacg cctcgtcctg gcgcaggttg
     2041 acctggatgc cggtcgtggc gccgtccggg ctgaccagca ggttgcggta caggtcgctg
     2101 tcgagcgcgc gccggcaatc ctgcacgcgg tccggcccgt tgcagttgag ctgctccggg
     2161 tcggtcggca ggtcggtcag gctgacgccc agtccgtcga tcagcggcac gtcgagcagc
     2221 gtcgtgaccg actcgacctc gtcgacggcc agcagcgcat cgcgcaactg ttccaggcgc
     2281 gccagggcgc gttccgtgaa caggccggcg tgcggcgtgt aggtgacgac gaggaagtca
     2341 tccgattcat agcgcgcgcg gatgatccgg taatagcgca ccgcctcgtc gtgctccatc
     2401 accagcgtct cggacgaggc atccagctgg aagcgcgggg cgaaagcacc gagcacggcc
     2461 accagcagcg ccgcggcgag cagcgtcagc accgggtggc ggatcaccgt gcggtcatat
     2521 gacgcgacca tggagcgctc gaggcgcctg aagatgccca tgcaggatcc ttgtgccgct
     2581 tttcggggct agttggccgg aacggccgcc gacaggtctt cgagcgcggc gcgcaactcg
     2641 atgagcttgg cgcgaatgat acccgcgttg tcgggcccga tgcgcagcag gaccttctgg
     2701 ccgggcgaga gcgcctccag cgacgggtcg gcgaaggtgt agaggacgtg cggttgcgcg
     2761 aggcggatcg ggccggtcgc gcgcggcgtc gcgagcaggt gatcgatgac ctcgagcgcg
     2821 cggttatgga actggcggcc cgggtagccg agctcttcgt aggcctgctg caacagcggg
     2881 taatgccgct ggtaggcggc ggccagcgcg acggcgtcca gcgcaccgac caggcgcacc
     2941 agcggcgcgt agcgggcatg gttggcgggg ccgatgacca gcccgtcctc ggggccctcg
     3001 gcaatgaagc ggccctcgat cggcggcacg gcccgcgcct tgatccacag cgcttcgcgc
     3061 ggcaggttgt cgaccgtcgc caccagcttg cggaccaggg cgtcgctgac caggtagcgc
     3121 tcgaccagcg tctccccggc cacctccgcg agcgcggcgc gtgccgcctc gtcgctctcg
     3181 tcgagcgacg gcaggggtgt tgcgggttcc accgcctctt cggcgacgaa ggccggcgcg
     3241 ggttcgggct ccgccggcgg tgccgcgggg ccgtcggcgg gatcggaagc ggccggcggc
     3301 tccggcagat ccaccgggac cggcgggagg tccgtggcgc ggcgcggata aaaaacggcc
     3361 gccagcccga
//
LOCUS       DAOMPG010000249         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11670_length_3332_cov_2.936222, whole genome shotgun sequence.
ACCESSION   DAOMPG010000249 DAOMPG010000000
VERSION     DAOMPG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11670_length_3332_cov_2.936222"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLS34_11270"
     CDS             <1..386
                     /locus_tag="PLS34_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRP88081.1"
                     /translation="EMLAAAREQGLFVLLEAFDEPDLERAAGLAAGGADPAAPVLVGV
                     NTRDLRTLAVDPERLARLAPHLPAGIAAVAESGLHTPADAAAAAALGYRLALVGTALM
                     RAADPERLAADMLAAGRRTVTEGAA"
     gene            383..973
                     /locus_tag="PLS34_11275"
     CDS             383..973
                     /locus_tag="PLS34_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRP88082.1"
                     /translation="MSLFVKICGLTTPEGVAAVVEAGADAAGFVFSPSPRQVAPALAR
                     QLAAGLPSAVRRVAVFRHPPPGWITRVLEEFPADWVQSDAADLPGVDLGGAESLPVFR
                     SGAPLPELLPELLLFEGPQSGAGNVADWRAAAALARRTGVILAGGLTPANVAAAIAQV
                     RPWGADVSSGVESSPGIKDPALVAAFVAAARRAEQG"
     gene            1002..2237
                     /gene="trpB"
                     /locus_tag="PLS34_11280"
     CDS             1002..2237
                     /gene="trpB"
                     /locus_tag="PLS34_11280"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRP88083.1"
                     /translation="MRVADAAGQLEALLDGRLPDARGRFGPWGGRFVPETLVPALDRL
                     QAGVAAHLHSAEFQACLGAELRDWVGRPTALTPAPRLSEAWGAELWLKREDLAHTGAH
                     KINNALGQAMLARMLGAQRVVAETGAGQHGVASAAACARIGLPCLVYMGEIDCERQAL
                     NVDRMRRLGAEVVPVTSGDRTLRAAIDEALRDWVSDPEGTYYLLGSVVGPHPYPYLVR
                     ELQAVIGREARGQVIERAGGLPDLVVACVGGGSNAIGLFHGFLGDPSVEILGIEAGGH
                     GTGLGENAATLSFGRPGVLHGTYSLLLSDDDGQVYETDSVSAGLDYPGVGPEHAFLHT
                     VGRVRYEYASDTEALAALDECCELEGILPALETAHAFAGAKRWARDHPGKRIVLGLSG
                     RGDKDMPTLAKFPKPERAR"
     gene            2234..3013
                     /gene="trpA"
                     /locus_tag="PLS34_11285"
     CDS             2234..3013
                     /gene="trpA"
                     /locus_tag="PLS34_11285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRP88084.1"
                     /translation="MSTGTSPHQGISAAIRAAGETGRTALVAFITAGYPDKARFREVL
                     AEVAAAADVVEVGVPFTDPMADGLTIQRASRAAIAQGVTLSWILDEIRAMDPPPAAPV
                     VLMSYLNPLLAMGYERLAARAVESGVAGFIVPDLPLDESAPLDAALAAQGLALIHLVS
                     PVTPPERIAAAARASRGFLYAVSVTGITGGAKSANANLKDYLRRVVAASTVPVCAGFG
                     IRSATQVADLAGVVPGVIVGSALVEALERGESPASFLAGLA"
     gene            3023..>3332
                     /locus_tag="PLS34_11290"
     CDS             3023..>3332
                     /locus_tag="PLS34_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HRP88085.1"
                     /translation="MPTAKNVLGEPLQPCSMEPLTGFYRDGCCNTGPHDIGRHVVCAR
                     VNAAFLEFSKSRGNDLSTPRPEHGFPGLSPGDRWCLCAARWKEALEAGCAPQVVLTAT
                     H"
BASE COUNT          406 a         1200 c         1273 g          453 t
ORIGIN      
        1 gcgagatgct ggcggcggcg cgcgagcagg ggttgttcgt gctgctcgag gccttcgacg
       61 agcctgacct cgagcgcgcc gcagggctgg cagcgggcgg cgcggatccc gccgcaccgg
      121 tcctggtcgg ggtcaacacc cgcgacctca ggactctcgc ggtggacccg gagcggctgg
      181 cgcgcctcgc gccgcacctg ccggccggca tcgctgcggt cgcggaaagc ggcctgcata
      241 ccccggcgga cgccgcggcg gcagccgcgc tcggctaccg gcttgcgctg gtgggcacgg
      301 cgctgatgcg cgcggccgac ccggagcggc tggcggccga catgctggcc gcgggccggc
      361 ggacggtgac ggagggcgcg gcatgagcct gttcgtgaag atctgcggct tgaccacgcc
      421 cgagggcgtg gcggccgtcg tcgaggccgg cgccgacgcc gccggcttcg tgttctcgcc
      481 ctcgccgcgc caggtagcgc cggcgctggc gcgccagctc gccgccggcc tgccgtccgc
      541 ggtgcgccgg gtcgcggtgt tccgccatcc gccgccgggc tggatcacgc gagtgctgga
      601 ggaatttccc gccgactggg tgcagagcga cgccgcggac ctgccgggcg tcgatctcgg
      661 cggcgccgag agcctgccgg tgttccgctc cggcgcgccg ctgccggaac tgttgccgga
      721 gctgctgctg ttcgaggggc cgcagagtgg cgccggcaac gtcgcggact ggcgggcggc
      781 ggcggcgctg gcgcgccgga ccggcgtcat cctcgccggc ggcctcaccc cggccaacgt
      841 cgcggcggcg atcgcgcagg tccggccgtg gggcgcggac gtctccagcg gcgtggagtc
      901 gtcgccgggc atcaaggatc ccgcgctggt ggcggccttc gtggccgcgg cccggcgggc
      961 ggaacaaggt tgaaggagca cagaccatga acagcacggc gatgcgcgtc gcggacgcgg
     1021 ccggacaact cgaggcgctg ctggacgggc gcctgcccga cgcgcggggc cgcttcggac
     1081 cgtggggcgg gcgtttcgtc cccgagaccc tggtgcccgc cctcgaccgg ctgcaggcag
     1141 gggtcgcagc gcacctgcac tcggcggaat tccaggcctg ccttggggcg gagctgcgtg
     1201 actgggtcgg ccggccgacg gcgctcacgc ccgcgccgcg cctgtccgag gcctggggcg
     1261 cggaactgtg gctgaagcgc gaggacctgg cccacactgg cgcgcacaag atcaacaacg
     1321 ccctcggcca ggcgatgctc gcccggatgc tcggcgcgca gcgcgtggtg gcggagaccg
     1381 gcgccggcca gcacggcgtg gcctcggccg cggcctgcgc gcgcatcggc ctgccctgcc
     1441 tggtgtacat gggcgagatc gattgcgagc ggcaggccct gaacgtcgac cgcatgcgtc
     1501 gcctcggcgc cgaggtggtg ccggtcacca gcggcgaccg caccctgcgc gccgccatcg
     1561 acgaggcgct gcgcgactgg gtgtccgacc cggagggcac gtattacctg ctgggctcgg
     1621 tggtcgggcc gcatccctat ccctacctgg tgcgcgagct gcaggcggtg atcggtcgcg
     1681 aggcgcgcgg ccaggtgatc gagcgcgccg gcggcctgcc ggacctggtg gtggcctgcg
     1741 tcggcggcgg cagcaacgcc atcggcctgt tccacggctt cctcggcgat ccgtcggtcg
     1801 agatcctcgg catcgaggcg ggcggccacg gcaccggcct cggtgagaat gcggcgacgc
     1861 tgagcttcgg ccggccgggc gtgctgcacg gtacctattc gctgctgctg tccgacgacg
     1921 acggccaggt gtacgagacc gacagcgtct cggccggcct cgactacccc ggcgtcgggc
     1981 cggagcacgc tttcctgcat accgtcggcc gcgtgcgcta cgagtacgcc agcgacaccg
     2041 aggcgctggc ggcgctcgac gagtgctgcg aactcgaggg catcctgccg gcgctggaga
     2101 cggcgcacgc cttcgccggc gccaagcgct gggcgcgcga ccatccgggc aagcgcatcg
     2161 tgctgggcct gtcgggccgc ggcgacaagg acatgccgac gctggcgaaa ttccccaagc
     2221 cggagcgcgc gcgatgagta ccgggaccag cccgcaccaa ggcatcagcg ccgcgatccg
     2281 cgccgccggc gaaacggggc gtaccgcgct ggtcgccttt atcaccgccg gctacccgga
     2341 caaggcccgc ttccgcgagg tgctggccga agtggcggcg gcggccgacg tcgtcgaggt
     2401 cggcgtgccc ttcaccgacc cgatggccga cggcctgacc atccagcgcg ccagtcgcgc
     2461 ggccatcgcc cagggcgtga cgctgtcgtg gatcctcgac gagatcaggg ccatggaccc
     2521 gccgccggcc gcgccggtgg tgctgatgag ctacctcaat cccctgctgg ccatgggcta
     2581 cgagcgcctc gcggcacgcg ccgtcgagag cggcgtggcc ggattcatcg tgccggacct
     2641 gccgctggac gagagcgcgc cgctcgatgc ggcgctggcc gcgcagggcc tggcgctcat
     2701 tcacctggtc agcccggtca cgccgccgga gcggatcgcc gccgcggcgc gcgccagccg
     2761 cggctttctc tacgcggtca gcgtcaccgg catcaccggc ggcgcgaaat cggccaatgc
     2821 gaacctgaag gactatctcc gccgggtggt ggccgcgtcc accgtgccgg tgtgcgcggg
     2881 cttcggcatt cgcagcgcga cgcaggtggc ggacctcgcc ggcgtggtgc cgggcgtgat
     2941 cgtcggttcg gcgctggtgg aggcgctgga gcgtggcgag tcgccggctt catttctcgc
     3001 cggcctggcc tagaattcag gcatgcccac cgcgaagaac gtcctcggcg aaccgctgca
     3061 gccttgcagc atggagccgc tgaccggctt ctaccgcgac ggctgctgca acaccgggcc
     3121 gcacgatatc gggcgccacg tggtgtgcgc gcgggtcaac gcggcctttc tcgagttctc
     3181 gaagtcgcgc ggcaacgacc tctccacgcc gcgccccgag cacggctttc ccggactctc
     3241 gccgggcgat cgctggtgcc tgtgtgcggc gcgctggaag gaggcgctgg aggccggctg
     3301 cgcgccgcag gtggtgctca ccgcgacgca cg
//
LOCUS       DAOMPG010000250         3293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11835_length_3293_cov_4.401174, whole genome shotgun sequence.
ACCESSION   DAOMPG010000250 DAOMPG010000000
VERSION     DAOMPG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11835_length_3293_cov_4.401174"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLS34_11295"
     CDS             complement(<1..269)
                     /locus_tag="PLS34_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRP88086.1"
                     /translation="MPELADPLRLPCGALLPNRIAKAAMTEGLAAADDFANARHARLY
                     RRWAAGGAGLLVTGNVMVDRRWLERPGNVVIEDRRSNAALAEWARA"
     gene            360..1613
                     /locus_tag="PLS34_11300"
     CDS             360..1613
                     /locus_tag="PLS34_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HRP88087.1"
                     /translation="MGEAGPLVTNDAVVLGLLALILGGVFWTQQSRHPFFQRFYTYVP
                     GLLLCYFLPSLLNTFGIIDGSQSRLYYVASRFLLPACLVLLTLSIDLPAVARLGRKAV
                     IMFFTGTAGIIIGGPLALLLVGTVSPATVGGEGPDAVWRGMTTIAGSWIGGGANQAAM
                     KEVFEVGDNLFGQMIAVDVLCASVWMAVLLFLAGRAQKIDARTGADTSAIEDLKRRIE
                     AYHARHARIPRLADLMFLAAVGFGCTALAHAGADFLAPWFSANTPQLERFSLTSGFFW
                     LVVIATTAGLLLSFTPARQLEGAGASTVGSAFLYILVASIGMQMDITAVLSNPGLFAV
                     GMVWIGFHAALLLIVGKLIKAPLFYLAVGSQANVGGAASAPVVASAFHPSLAPVGVLL
                     AVLGYAVGTYGAWLCGQILRVVAAG"
     gene            1642..2097
                     /locus_tag="PLS34_11305"
     CDS             1642..2097
                     /locus_tag="PLS34_11305"
                     /inference="COORDINATES: protein motif:HMM:NF040030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HRP88088.1"
                     /translation="MALSAQDCVATLLADYAPASLLVVGDPPTAPETDAELTCVSVHE
                     VAAELAVLARFDFAVITELLEELDGPTAEFVLGRLKNLHTDKYLLLADPARACLGHDA
                     LLALALAPCEQLDDGRIAWRYDIDRYNPERPWNNPEDWAHPQNFDRFRW"
     gene            2128..2331
                     /locus_tag="PLS34_11310"
     CDS             2128..2331
                     /locus_tag="PLS34_11310"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HRP88089.1"
                     /translation="MPVIQCDIREGRSEEQKQALAREITRVVHETIGAPVEYIYVLIR
                     EQPGRHHVKGGKALPDYVPDAGA"
     gene            2380..>3293
                     /locus_tag="PLS34_11315"
     CDS             2380..>3293
                     /locus_tag="PLS34_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP88090.1"
                     /translation="MSDPLPLGHKEHVVRGMAAALGAFFMFTVMNVFAKLLSAHHSVI
                     EIAFYRNLIASLPFLFMVFVLGRREILVIRSKPSLVGLRAVLGAVSLITTFAAFSLMP
                     MAETTVLLFTASLFIPVLGVFFLGERVGPWRWAAVVIGFAGVVVMSQPGGSVHLLGVT
                     VALTAALMHATLQIVLRYLGRYESPETVTFYFMVIGTLVTALPLPFVAVTPTLAEVPL
                     LFGVGLSGALAQWLLSVAFRNAPAAIVTVFNYSGIVWATAFGWLIWNDWPLPTVLFGA
                     AIVIASNALIIWRESRRRAITGARVRAK"
BASE COUNT          438 a         1233 c         1076 g          546 t
ORIGIN      
        1 gccctcgccc actccgccag cgccgcgttg ctgcgccggt cctcgatgac cacgttgccg
       61 gggcgctcca gccagcgccg gtccaccatc acgttgccgg tcacgagcag acccgcgccg
      121 cccgccgccc agcggcgata cagccgcgca tggcgcgcgt tggcgaagtc gtccgccgcc
      181 gccaggccct cggtcatggc cgccttggcg atgcggttcg gcagcagcgc gccgcacggc
      241 aatctgagcg gatcggcaag ctcgggcatc aacacaccgc ggggcaaaag ccagcgcgca
      301 gcatagcctg cccgccgctc gggtagaatc ggccgccagc gacaaggaga acaccaccca
      361 tgggcgaagc cggaccgctg gtcaccaacg acgccgtcgt gctcgggctg ctcgccctga
      421 tcctcggcgg cgtgttctgg acgcagcaaa gccgccaccc cttcttccag cgcttctaca
      481 cctacgtgcc cgggctgttg ctgtgctact tcctgccctc gctgctgaac accttcggca
      541 tcatcgacgg cagccagtcg cgactctatt acgtcgcctc gcgcttcctg ctgcccgcct
      601 gcctggtgct cctgacgctg tccatcgacc tgccggcggt ggcgcggctc ggccgcaagg
      661 ccgtcatcat gttcttcacc ggcacggcag ggatcatcat cggcggccca ctggcgctgc
      721 tgctggtcgg caccgtcagc ccggccacgg tcggcggcga gggcccggac gccgtctggc
      781 gcggcatgac cacgatcgcc ggcagctgga tcggcggtgg cgccaaccag gcggcgatga
      841 aagaggtttt cgaggtcggc gacaacctgt tcggccagat gatcgccgtg gacgtgctgt
      901 gcgccagcgt gtggatggcc gtgctgctgt tcctcgccgg gcgggcgcag aagatcgacg
      961 cgcgcaccgg cgccgacacc tccgccatcg aggacctcaa gcgccgcatc gaggcctacc
     1021 acgccaggca cgcgcgcatc ccgcgcctcg ccgacctgat gtttctggcc gcggtcggct
     1081 tcggctgcac cgccctggcg cacgccggcg ccgatttcct cgccccctgg ttcagcgcca
     1141 acacgccgca gctggagcgc ttcagcctca ccagcggctt cttctggctg gtcgtcatcg
     1201 ccaccaccgc cgggctgctg ctgtccttca cgccggcgcg ccagctcgag ggcgccggcg
     1261 cctccacggt cggctcggcc ttcctgtaca tcctggtcgc cagcatcggc atgcagatgg
     1321 acatcaccgc ggtgctctcc aaccccggcc tgttcgcggt cggcatggtg tggatcggct
     1381 tccacgccgc cctgctgctg atcgtcggca agctcatcaa ggcaccgctg ttctacctcg
     1441 cggtcggcag ccaggcgaac gtcggcggcg cggcgtccgc gcccgtggtg gcctccgcct
     1501 tccatccctc gctggcgccg gtcggcgtgc tgctggccgt gctcggctat gccgtcggca
     1561 cctacggcgc gtggctgtgc ggccagatcc tgcgcgtcgt cgccgccgga tgagtggcga
     1621 gcgcatgaga gccgcgggct gatggcgctc tcggcgcagg attgcgtggc gacgctgctg
     1681 gcggactacg cgcccgccag cctgctggtc gtcggcgacc cgccgacagc gcccgagacg
     1741 gacgccgagc tgacctgcgt ctccgtgcac gaggtggcgg ccgagctggc cgtgctcgcg
     1801 cgcttcgact tcgcggtcat caccgagctg ctcgaagaac tcgacggccc gaccgcggag
     1861 ttcgtgctcg gccggctgaa gaacctgcac accgacaagt acctgctgct ggccgacccg
     1921 gcgcgtgcct gcctgggaca cgacgccctg ctggcgctcg cgctggcgcc ctgcgagcag
     1981 ctcgacgacg gccgcatcgc gtggcgttac gacatcgacc gctacaaccc cgagcggccg
     2041 tggaacaatc ccgaggactg ggcgcacccg cagaacttcg accggttccg gtggtagccc
     2101 gtccacgcaa tacttgagga gcccgacatg cccgtcatcc agtgcgacat ccgcgaaggc
     2161 cgcagcgagg agcagaagca ggcgctcgcc cgcgagatca cgcgcgtggt ccacgagacc
     2221 atcggcgcgc cggtcgagta catctacgtg ctcatccgcg agcagcccgg ccgccaccac
     2281 gtcaagggcg gcaaggccct gccggactac gtgccggacg ccggcgcctg acgggctgcc
     2341 ggggcgtccc gtccgcaccg ccgcccgccg cccgccccga tgagcgaccc cctgccgctc
     2401 ggtcacaagg aacacgtcgt ccgcggcatg gcggcggcgc tgggcgcgtt cttcatgttc
     2461 accgtgatga acgtgttcgc caagctgctc agcgcccacc actccgtcat cgagatcgcc
     2521 ttctaccgca acctgatcgc cagcctgccg ttcctgttca tggtattcgt gctcggccgg
     2581 cgcgagatcc tggtgatccg cagcaagccc tcgctggtcg gcctgcgtgc ggtgctgggc
     2641 gcggtgtcgc tgatcaccac cttcgccgcc ttctcgctca tgcccatggc cgagaccacc
     2701 gtgctgctgt tcaccgcctc actgttcatc cccgtactcg gcgtgttctt cctcggtgag
     2761 cgcgtcgggc cgtggcgctg ggccgcggtg gtcatcggct tcgccggcgt cgtggtgatg
     2821 tcgcagccgg gcggcagcgt gcacctgctc ggcgtcaccg tggcgctgac cgccgcgctg
     2881 atgcacgcca ccctgcagat cgtgctgcgc tacctcggcc gctacgagag ccccgagacc
     2941 gtcacctttt atttcatggt catcggcacg ctggtcaccg ccctgccgtt gcccttcgtc
     3001 gccgtcacac cgaccctggc cgaagtgccg ctgctgttcg gtgtcggact ctcaggggcg
     3061 ctggcgcagt ggctgctgtc agtggccttc cgcaacgcac cggcggcgat cgtcacggtg
     3121 ttcaactaca gcggcatcgt ctgggcgacg gccttcggct ggctgatctg gaacgactgg
     3181 ccgctgccga cggtgctgtt tggcgccgcc atcgtgatcg cctccaacgc gctgatcatc
     3241 tggcgcgaaa gccgccggcg cgcgatcacg ggagcgcggg tgcgggcgaa gtt
//
LOCUS       DAOMPG010000251         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11895_length_3280_cov_3.881550, whole genome shotgun sequence.
ACCESSION   DAOMPG010000251 DAOMPG010000000
VERSION     DAOMPG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_3280_cov_3.881550"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLS34_11320"
     CDS             <1..409
                     /locus_tag="PLS34_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88091.1"
                     /translation="MPGYTLAIGEAIMPAMAAWVVIPFGPEVALSNVDAGLLLFSPAV
                     HWYAGFSGVLHGLLAAGAIVGLGRTPAMAGLLLGGLVLKLVLEQLGLGTTGTAQLIGA
                     SVVVDAHLYGALAGGVGALSLRTWRSRSTSAPR"
     gene            complement(369..2261)
                     /locus_tag="PLS34_11325"
     CDS             complement(369..2261)
                     /locus_tag="PLS34_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HRP88092.1"
                     /translation="MGYDQIYQHSIREPETFWAEAARAIAWDKPWSQVLDADKAPLYR
                     WFKGGSLNSCHNALDRHVDAGRGDQVALIYDSPVTGTKAQFSYRELRDRVARLAGGLA
                     SLGVQKGDRVILYMPMVPEAVIGMLACARLGAVHSVVFGGFASRELATRIDDAKPKVI
                     LSASCGIEVNRVVPYKPLLDEAIRSATHQPEHCVILARPEAPSDLKQGRDLDWAELEA
                     ESEPADCVSVAATDPLYILYTSGTTGLPKGVVRDNGGHAVALAWSMGHVYGVKPGEVF
                     WAASDVGWVVGHSYIVYAPLFHGCTTVLYEGKPVGTPDAGAFWRVIAEYGVSVMFTAP
                     TAFRAIKRDDPKGEFIARYDLGKLRALFLAGERCDPDTLNWAMEKLGIPVIDHWWQTE
                     TGWPIAANCLGIEQLPVKPGSPTRAVPGYDVRVLGDDGKELPDGEIGSIMIRLPLPPG
                     TLPTLWGNDQRYIDSYLSAHPGWYLTGDAGYRDEDGYLWIMSRIDDIINTAGHRLSTG
                     AMEEVLAAHPDVAEAAVIGAADELKGELPVGLVVLKSGVQRGHDDIKADLVRMVRERI
                     GPVAAFKKVAIVDRLPKTRSGKVLRATMRKIADGQEYRVPPTIDDPAILDEVTEALKS
                     IGYARS"
     gene            2496..3137
                     /gene="ccmA"
                     /locus_tag="PLS34_11330"
     CDS             2496..3137
                     /gene="ccmA"
                     /locus_tag="PLS34_11330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HRP88093.1"
                     /translation="MSVAPGSGPVLLEGRNLELWRGEARLFTGVSFELRAGELLHIVG
                     PNGCGKTSLLRVLCGLTLPETGDVYWRSKKVSRNRADFHASLCYLGHRESLKADLTAE
                     ENLVFQLGLRAWLSSADIHAALDKVGLSRRRNVPARSLSAGQKRRVSLAKALASGAKL
                     WVLDEPYTNLDVAGRELVDQMMSEHLERNGLVLLVAHQAHGVGAGVTRRLELG"
     gene            3137..>3280
                     /locus_tag="PLS34_11335"
     CDS             3137..>3280
                     /locus_tag="PLS34_11335"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRP88094.1"
                     /translation="MEWPGLGTAFWLQLTRDLRLAFRHIGQAANPLMFFVMVTTLFPL
                     ALSP"
BASE COUNT          460 a         1120 c         1156 g          544 t
ORIGIN      
        1 catgcccggg tacacgctgg caatcggcga ggcgatcatg ccggcgatgg ccgcgtgggt
       61 ggtcattccc ttcggacccg aggtcgcgct gtcgaacgtc gacgccggcc tgctgctgtt
      121 ttcaccggcc gtccactggt acgccggttt ctccggcgtc ctgcacggcc tgctggcggc
      181 tggcgcaatc gtcggcctcg gccgcacgcc ggcgatggcg gggctgctgc tgggcgggct
      241 ggtgctgaaa ctggtgctcg agcagctggg gctcggcacg acgggcacgg cgcaactgat
      301 cggcgcgtcc gtggtggtgg acgcgcacct ctacggcgcg ttggccggcg gggtcggcgc
      361 gctgtcgctc aggacctggc gtagccgatc gacttcagcg cctcggtgac ttcgtcgagg
      421 atcgccgggt cgtcgatcgt cggcggtaca cggtactcct ggccgtcggc gatcttgcgc
      481 atggttgccc gcagcacctt gccggagcgc gtcttgggca agcgatcgac gatggccacc
      541 ttcttgaacg ccgccaccgg cccgatgcgc tcgcgcacca tcctcacgag gtccgccttg
      601 atgtcgtcgt gcccgcgttg cacgccggac ttcagcacca caagcccgac cggcagctcg
      661 cccttgagct cgtcggcggc gccgatcacg gcggcctccg cgacgtccgg gtgcgccgcc
      721 agcacttctt ccatggcgcc cgtcgaaagg cgatgcccgg ccgtattgat gatgtcgtcg
      781 atgcggctca tgatccacag gtagccgtcc tcgtcgcgat aaccggcatc gcccgtcagg
      841 taccagccgg gatgggcgct gaggtaggaa tcgatgtagc gctggtcgtt gccccacagc
      901 gtcggcaggg tgcccggcgg cagcggcagg cggatcatga tgctgccgat ctcgccatcc
      961 ggcagttcct tgccgtcgtc gcccagcacc cgcacgtcgt agcccggcac cgcccgcgtc
     1021 ggggagcccg gcttgacggg gagctgctcg atgccgagac agttggccgc gatcggccag
     1081 ccggtttccg tctgccacca gtgatcgatg accggaatgc ccagtttctc catcgcccag
     1141 ttgagggtgt ccgggtcgca acgctcgccg gcgaggaaca gggcgcgcag cttgccgagg
     1201 tcgtagcgcg caatgaattc acctttcgga tcgtcgcgct ttatggcgcg aaaggcggtc
     1261 ggcgcggtga acatcacgct cacgccgtac tcggcgatca cccgccagaa ggcgccggcg
     1321 tcaggcgtgc cgaccggctt gccctcgtac agcacggtgg tgcagccgtg gaacagcggc
     1381 gcatacacga tgtagctgtg gccgaccacc cagccgacgt ccgaggccgc ccagaacact
     1441 tcgcccggct tcacgccgta cacgtgaccc atgctccacg ccagcgcgac ggcatggccg
     1501 ccgttgtcgc gcaccacgcc cttgggcagg ccggtcgtgc cggaggtgta gagaatgtac
     1561 agcgggtcgg tggcggcgac gctgacgcag tcggccggct cgctttccgc ctcgagctcg
     1621 gcccagtcga ggtcgcggcc ctgcttgaga tcggagggcg cctcgggacg ggccaggatg
     1681 acgcagtgct cgggctggtg cgtggcgctg cggatcgcct cgtcaagcag cggcttgtaa
     1741 ggcaccaccc ggttgacctc gatgccgcag gacgcggaca ggatgacttt cggcttggcg
     1801 tcgtcgatgc gggtcgccag ctcgcgcgag gcgaagccgc cgaacaccac cgagtggacg
     1861 gcgccaagcc gggcgcaggc cagcatgccg atcaccgcct cgggcaccat gggcatgtaa
     1921 agaatgacgc ggtcaccttt ctgcacaccg aggctggcca gcccgccggc caggcgcgcg
     1981 acgcggtcgc gcagctcgcg gtaactgaac tgtgccttgg tgcccgtgac cgggctgtcg
     2041 tagatcaggg cgacctggtc gccgcgcccg gcgtccacgt ggcggtccag cgcgttgtga
     2101 cagctgttga gcgagccgcc cttgaaccag cgatacagcg gcgccttgtc ggcgtcgagg
     2161 acctggctcc agggcttgtc ccacgcgatg gcgcgcgccg cctccgccca gaatgtctcg
     2221 ggttcgcgga tggagtgctg gtagatctgg tcgtatccca tggtcgtccc cctgcgtgcc
     2281 ttcgtcggcg acctgccagg cacggcccct gcgggcctcc ctgatgcgtc gccccttcgg
     2341 ggggagtgta tactgcgcgg cgcccggagg ccgactccgg gggcgaaagc tggcgcagtg
     2401 ccgccggttc cgcctgtcca tcctggtcga gcacggagag ctgcattgcc agcaagagtc
     2461 atccagggag ccgcctgcct gcggcggcgc ccgtcatgag cgttgcgccc ggcagcggcc
     2521 cggtcctcct ggagggcagg aacctcgagc tgtggcgcgg cgaggcgcgg ctcttcaccg
     2581 gcgtctcgtt cgagctgcgc gccggcgaat tgctgcacat cgtgggcccg aacggctgcg
     2641 gcaagaccag cctgttgcgg gtgttgtgcg gtctgacgct gccggagacg ggcgacgtct
     2701 actggcgcag caagaaggtg tcgcgcaacc gcgctgattt tcacgccagt ctctgctatc
     2761 tggggcaccg cgagagcctc aaggcggatc tcaccgccga ggaaaacctg gtattccagc
     2821 tcggcctgcg ggcctggctc agttcggcgg acatccatgc ggcgctcgac aaggtcggac
     2881 tgtcccggcg gcgcaacgtc ccggcgcgca gcctgtccgc cggccagaag cggcgtgtct
     2941 cgctggcgaa ggcgctggcc agcggcgcca agctgtgggt gctcgacgag ccctacacga
     3001 acctcgacgt ggccggccgc gagctggtcg accagatgat gagcgagcac ctcgagcgca
     3061 acggcctggt gctgctggtg gcgcaccagg cgcacggcgt cggcgccggc gtgacgcgcc
     3121 ggctggagct gggctgatgg agtggccagg actcggcacg gctttctggc tgcaattgac
     3181 gcgcgacctc aggctcgcgt tccggcacat cggccaggcc gccaacccgc tgatgttctt
     3241 cgtcatggtg accacgctgt tcccgctggc gctgagcccg
//
LOCUS       DAOMPG010000252         3264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_11969_length_3264_cov_4.335619, whole genome shotgun sequence.
ACCESSION   DAOMPG010000252 DAOMPG010000000
VERSION     DAOMPG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_3264_cov_4.335619"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="lepA"
                     /locus_tag="PLS34_11340"
     CDS             complement(<1..1055)
                     /gene="lepA"
                     /locus_tag="PLS34_11340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRP88095.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRFIQVCGGLADREMSAQVLDSM
                     DIERERGITIKAQAVSLRYRARDGRDYELNLIDTPGHVDFSYEVSRSLAACEGAILIV
                     DAAQGVEAQSVANCYTAVEAGLEVIPVINKIDLPSADPDRVKNEIEEIIGIEAKSAVL
                     TSAKTGQGVEDVLDALIARIPPPQGDPDGPLQALIIDSWFDNYVGVVSLVRVMNGVLE
                     KGARIRIMSTGRSHNADRVGRFTPKMTDRDRLETGQVGYLISGIKEIDGAPVGDTVTL
                     ESRPCPKPLPGFKQVQPRVFAGLFPINSEDYEDFREALQKLKLNDSALHFEPETSTAL
                     GFGFRCGFLGMLHMEIV"
     gene            complement(1121..1369)
                     /locus_tag="PLS34_11345"
     CDS             complement(1121..1369)
                     /locus_tag="PLS34_11345"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRP88096.1"
                     /translation="MSAPAPEVLIYTRRDCSLCERMEAGVRELLGPDAGVTLVEIDDD
                     PGLLARFGRDVPVLCVDDEVVCRHFLDAGRLRAAFPPS"
     gene            complement(1366..1830)
                     /locus_tag="PLS34_11350"
     CDS             complement(1366..1830)
                     /locus_tag="PLS34_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRP88097.1"
                     /translation="MITETGRVLAVEGDWAWVECRRQAECARCAEGRGCGGGVLGKLL
                     GDRLHKVRAATGSVAVEPGDQVLIGLGEDAVMRAAAAVYLVPLLFALAAGVAAATLAG
                     GSDLAALAGAVAGLVLGLGWARDYSRRHAADSALQPVILRHADGAGCAGRPS"
     gene            complement(1827..2822)
                     /locus_tag="PLS34_11355"
     CDS             complement(1827..2822)
                     /locus_tag="PLS34_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HRP88098.1"
                     /translation="MIDARFRQGLLLAALLSALPAAALAAEARAWLERMEEAVETLSY
                     EGTFVHMIGSRIETMHVIHRAVDGQIAERLYSRDQPGREVLRHNDKVTCIFADERTVL
                     VEQRRGRGGAPLLGAVPKDAAGIEEWYEFRVAGEDRKLGRSATIIEIRPLDGYRYGHR
                     LWLDELTAMPLKVQLLDPQGGALEQIQFVSMVVPAEISDARLLPDVSIEGFTWYQQES
                     APATPGVEDPPVGWSLEDPPPGFQLSEFQRRTLPGADQPVEHLVYSDGLASVSVFIEP
                     AEATDRELSGLSRMGAAHAFTLILDGRQITAVGEVPPQTVQRMARALRPAAGAAH"
     gene            complement(2819..>3264)
                     /locus_tag="PLS34_11360"
     CDS             complement(2819..>3264)
                     /locus_tag="PLS34_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88099.1"
                     /translation="GRPVDLVARVSAALAAGPAAATVAPKRMRHLARYGAGLAVAASV
                     AMVALLALPGRQQVEAPPMLSATEVASPAPEQRMMLPPVYTRAAGGGPDRLTRYYVNH
                     TGFAPPVSGRGALTRIIIIQPEAATTEEERAAQEAETREEHGIAR"
BASE COUNT          460 a         1191 c         1145 g          468 t
ORIGIN      
        1 tgcacgatct ccatgtgcag catgccgagg aagccgcagc ggaagccgaa gcccagcgcg
       61 gtcgaagtct cgggctcgaa atgcagcgcc gagtcgttca gcttgagctt ctgcagggcc
      121 tcgcggaagt cttcgtaatc ctcggaattg atcgggaaca gcccggcgaa cacccgcggc
      181 tgcacctgct tgaagccggg caacggcttc gggcagggcc gcgattccag ggttacggta
      241 tcgccgaccg gggcgccgtc gatttccttg atgccggaga tcaggtagcc gacctggccc
      301 gtttccagcc ggtcgcggtc ggtcatcttg ggcgtgaagc gccccacccg gtccgcattg
      361 tggctgcggc cggtggacat gatgcgaatg cgggcgccct tttccagcac gccgttcatc
      421 acccgcacca gcgagactac gccgacgtag ttgtcgaacc aggagtcgat gatcagcgcc
      481 tgcagcggcc cgtccgggtc gccctgcggc ggcgggatgc gcgcgatcag cgcatccagc
      541 acgtcctcca cgccctggcc cgtcttggcg ctggtcagaa cggcgctctt cgcctcgatg
      601 ccgatgatct cctcgatctc gttcttgacg cgatcgggat cggccgaggg caggtcgatc
      661 ttgttgatca ccgggatgac ctcgaggccc gcctccaccg ccgtgtagca gttggcgacg
      721 ctctgcgcct cgacgccctg cgcggcgtcc acgatcagga tggcgccctc gcaggcagcc
      781 agcgagcggg acacctcgta ggagaaatcg acgtgcccgg gcgtgtcgat caggttcagc
      841 tcgtagtccc ggccgtcgcg cgcccggtag cgcagcgata ccgcctgcgc cttgatggtg
      901 atgccgcgct cccgctcgat gtccatcgag tcgagcacct gtgccgacat ctcgcggtcg
      961 gccagcccgc cgcatacctg gatgaaccgg tccgcgagcg tggacttgcc gtggtcgatg
     1021 tgggcgatga tggaaaaatt gcggatatgg tccatgggcg cacgcaacac gccggcgtcg
     1081 cgccggccgg gcattatacc ggtgccggaa cccgcgcgcc ctagcttggc ggaaaggccg
     1141 cgcgcagccg cccggcgtcg aggaaatgcc tgcagaccac ctcgtcgtcg acgcacagca
     1201 cgggcacgtc gcgcccgaag cgcgccagca gccccgggtc gtcgtcgatc tcgaccagcg
     1261 tgacgcccgc atccggcccg agcagctcgc gcacgccggc ctccatccgc tcgcacagcg
     1321 agcagtcccg gcgcgtgtag atcaacacct cgggcgccgg ggcgctcacg acggcctgcc
     1381 ggcgcagccc gcgccgtccg catgcctcag gatcaccggc tggagcgcgc tgtcggccgc
     1441 atggcggcgg ctgtagtcgc gcgcccagcc gaggccgagc accagcccgg cgacggcgcc
     1501 cgccagcgcg gccaggtcgc tgcccccggc cagggtggcg gccgccaccc cggcggccag
     1561 ggcaaacagc aggggcacca ggtagacggc agccgcggcg cgcatcaccg cgtcctcgcc
     1621 gaggccgatc agcacctggt cgcccggctc gaccgccacc gagccggtcg cggcccggac
     1681 cttgtgcagc cggtcgccga gcagcttgcc gagcacgccg ccgccgcagc cgcggccctc
     1741 ggcacagcgc gcgcactcgg cctggcggcg gcattcgacc caggcccagt cgccctccac
     1801 cgccagcacc cgcccggtct cggtgatcaa tgcgcggcgc ccgcggcggg ccggagcgcc
     1861 cgcgccatgc gctgcacggt ctgcggcggc acttctccga ccgccgtgat ctgccgaccg
     1921 tcgagaatca gggtgaaggc gtgcgccgcg cccatgcgcg acaggcccga cagctcgcgg
     1981 tcggtggcct cggccggttc gatgaacacg gagaccgagg ccagcccgtc ggaatagacc
     2041 aggtgctcga cgggctggtc tgcgccgggc agcgtgcgcc gctggaattc gctcagctgg
     2101 aagcccggcg ggggatcctc cagcgaccag ccgactggcg gatcctccac gccgggcgtg
     2161 gccggtgccg attcctgctg gtaccaggtg aatccctcga tggagacgtc cggcagcagc
     2221 cgggcatccg agatctcggc cggcaccacc atgctgacga actggatctg ctccaaggcc
     2281 ccgccctgcg ggtcgagcag ctggaccttc agcggcatgg ccgtgagctc gtccagccac
     2341 aggcggtggc cgtagcggta gccgtcgagc ggccggattt cgatgatcgt ggcgctgcgt
     2401 cccagcttgc ggtcctcgcc cgccacgcga aactcgtacc attcctcgat gccggcggcg
     2461 tccttgggca ccgcgcccag cagcggcgcg ccgccgcgcc cgcggcgctg ctcgaccagc
     2521 accgtgcgct cgtcggcgaa tatgcaggtg accttgtcgt tgtggcgcag cacttcacgg
     2581 ccgggctggt cgcgcgagta cagccgctcg gcgatctgcc cgtccaccgc ccggtggatg
     2641 acgtgcatcg tctcgatgcg gctgccgatc atgtgcacga aggtaccctc gtagctgagg
     2701 gtctcgaccg cctcctccat gcgctccagc cacgcgcgtg cctcggccgc aagcgccgct
     2761 gccggcaggg ccgacagcaa cgcagcgagc agcaggccct gccggaagcg cgcgtcgatc
     2821 atcgggcgat cccgtgctcc tcgcgggtct ccgcttcctg cgcggcgcgc tcttcctcgg
     2881 tggtggcggc ctcgggctga atgatgatga tgcgggtcag ggcgccgcgg ccgctcacgg
     2941 gcggggcgaa gccggtgtga ttgacgtaat agcgcgtcag ccggtcgggg ccgccgccgg
     3001 cggcacgggt gtagacgggt ggcagcatca tgcgctgctc gggcgcgggg gacgccactt
     3061 cggtggcgct cagcatgggc ggcgcttcga cctgctggcg gccgggcagt gccagcagcg
     3121 cgaccatcgc gaccgaggcg gccaccgcca gcccggcgcc gtagcgggcg aggtggcgca
     3181 tgcgcttcgg cgcgacggtc gcggccgccg gtccggcggc cagcgccgcg ctgacccggg
     3241 cgaccaggtc gaccggccgg cccg
//
LOCUS       DAOMPG010000253         3243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12070_length_3243_cov_4.159661, whole genome shotgun sequence.
ACCESSION   DAOMPG010000253 DAOMPG010000000
VERSION     DAOMPG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12070_length_3243_cov_4.159661"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLS34_11365"
     CDS             complement(<1..767)
                     /locus_tag="PLS34_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP88100.1"
                     /translation="MTPSAPRYRDLLHDGLARAAARRPDKIALHVGNESMSYGELDAQ
                     SDRLAAALQARGLARGDRVAIFMDNTLPCAVAIFGVLKAGGVFLVINPQTKHDKLCFI
                     ANDCAVRHLVTDIHLANVAGAVVGAVPSLTHILVSGRGECLDALSGPGVTTEWLDEGL
                     AGAVPEMRPPATIPTDLASLIYTSGSTGNPKGVMQTHQSMAFAVGSLVEYQRVDEDDV
                     LINMLPMAFDYGLYQLLMSVATGATLVHPYTDPRVIAG"
     gene            complement(779..1030)
                     /locus_tag="PLS34_11370"
     CDS             complement(779..1030)
                     /locus_tag="PLS34_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRP88101.1"
                     /translation="MSTEEKLRKYILETYLFTTDDSALANDDSFLDKGIIDSTGILEL
                     VMYLEEEFGLAVADEELLPDNLDSINNLVKFVERKQAAA"
     gene            complement(1058..3016)
                     /gene="asnB"
                     /locus_tag="PLS34_11375"
     CDS             complement(1058..3016)
                     /gene="asnB"
                     /locus_tag="PLS34_11375"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRP88102.1"
                     /translation="MCGLAGILHLDGGGDVSRRELEPMVAALASRGPDGTGYYTAPGL
                     GLGHARLSIIDLEGGDQPIHNEDKSVWTVFNGEIFNYVELRADLESRGHRFYTHTDTE
                     VIVHLYEEYGDDFPNHMNGQFAIALWDAPRRRLLLVRDRPGILPLYFTREGNRLLFAS
                     EVKALLPAMRRRPELDAAALNQLMTFWAPVSPASVFKGVEEVSPGEMLIVEHGTLRRQ
                     RYWEWTFPEDGEYLPGSVAELTEELHDLLLDVTRLRLRADVPVGAYLSGGLDSSGIAG
                     LVKHSGHPQLRTFSIRFEDPGVDESEHQERMSRHLGTDHTSILCRNADIAAIFPRIIA
                     RTEAPVLRTAPAPMAILSGLVRQQDYKVVLTGEGADEVLGGYDLFKETKIRLFWARQP
                     DSKWRPALLKRLYPYLDLNQRTGAAYLRSFFGQGLENPDAMGFSHLPRWTTTGKTRDF
                     FSPAFRATITDDPEATLAARLPAGFPAWHPFNRAQFLEARTLMAGYLLNSQGDRMLMA
                     NSVEGRFPFLDHRVIEFANRLHPTMKMRGLNEKYLLKKALARHLPKETVARFKQPYRA
                     PDIAAFFGPDGPPEYVRDCLSPASLADTGYFDAQRVQHLLKKIEHGRVIGFKDNMAFL
                     AVLSTQLWHRNFVEGRDLPVPEAVAGAR"
BASE COUNT          501 a         1023 c         1185 g          534 t
ORIGIN      
        1 ccggcgatca cgcgcgggtc ggtgtagggg tggacgaggg tggcgccggt cgcgaccgac
       61 atcaacagct ggtacaggcc gtagtcgaag gccatcggca gcatgttgat cagcacgtcg
      121 tcttcatcga cgcgctggta ctcgaccagg ctgcccaccg cgaaggccat ggactggtgg
      181 gtctgcatga cgcctttcgg gttgccggtg ctgccggagg tgtagatcag cgaggcgagg
      241 tcggtcggga tggtggccgg gggacgcatc tccggaacgg ccccggccag cccctcgtcg
      301 agccactcgg tcgtcacccc gggaccggac aaggcgtcga ggcattcgcc gcggccggaa
      361 acgagaatat gcgtcaggct cggcaccgcg cccaccaccg cgccggccac gttcgccagg
      421 tggatgtcgg tgaccaggtg gcggacggcg cagtcgttgg cgatgaagca cagcttgtcg
      481 tgcttggtct ggggattgat caccaggaac acgccgccgg ccttgagcac gccgaagatg
      541 gcgaccgcgc agggcagggt gttgtccatg aagatcgcca cgcggtcgcc gcgcgccagg
      601 ccgcgcgcct gcagggcggc ggcgagccgg tcggactgcg cgtccagctc gccgtagctc
      661 atggactcgt tgccgacgtg cagggcgatc ttgtcgggac gccgggcggc ggcgcgggcg
      721 aggccgtcgt gcagcaggtc gcggtagcga ggcgcggagg gcgtcatggg acggggcctt
      781 aggcggcggc ctgcttgcgc tcgacgaact tcaccaggtt gttgatggag tcgaggttgt
      841 ccggcagcag ctcctcgtcg gcgacggcca ggccgaactc ctcttcgagg tacatgacca
      901 gctcgaggat gccggtggag tcgatgatgc ccttgtccag gaacgagtca tcgttcgcca
      961 gggcgctgtc gtccgtggtg aacaggtagg tctcgaggat gtacttcctg agtttttctt
     1021 ctgtcgacat gaatacggtt cctggcggat cgggtggtca acgggcgccg gcgacggcct
     1081 cgggcacggg caaatcccgt ccctcgacga agttccggtg ccaaagctgc gtggagagta
     1141 cggcaagaaa ggccatgttg tctttgaaac cgatcacacg gccatgctcg atcttcttca
     1201 gcaggtgctg cacccgctgc gcgtcgaaat agcctgtatc cgccagcgaa gcggggctga
     1261 ggcagtcgcg cacgtactcc ggcgggccgt ccgggccgaa gaaggcggcg atgtcgggcg
     1321 cccggtaggg ctgcttgaag cgcgccaccg tctctttcgg caggtggcgc gcgagcgcct
     1381 tcttgagcag gtacttctcg ttcagccccc gcatcttcat ggtcgggtgc aggcggttgg
     1441 cgaactcgat gacgcggtgg tcgaggaagg ggaagcggcc ctccacggag ttggccatca
     1501 gcatgcggtc gccctggctg ttgagcaggt acccggccat cagcgtcctg gcctcgagga
     1561 actgggcgcg gttgaaggga tgccaggcgg ggaagccggc cggcagccgc gcggccagcg
     1621 tggcctccgg gtcgtccgtg atggtggcgc ggaacgccgg gctgaagaaa tcgcgggtct
     1681 tgccggtggt ggtccagcgc ggcaggtgcg agaagcccat ggcgtccggg ttctccagcc
     1741 cctggccgaa gaagctcctg aggtacgccg cgccggtgcg ctggttgaga tcgagatagg
     1801 gatacagccg cttgagcagg gcggggcgcc acttcgaatc gggctggcgg gcccagaaca
     1861 ggcggatctt ggtttccttg aagaggtcgt agccgccgag cacctcgtcg gcgccctcgc
     1921 cggtcagcac caccttgtag tcctgctggc gcaccaggcc ggagaggatg gccatcggcg
     1981 cgggcgcggt gcgcagcacc ggcgcctcgg tgcgggcgat gatgcgcggg aagatcgcgg
     2041 cgatgtcggc gttgcggcac aggatggagg tgtggtcggt gccgaggtgg cggctcatgc
     2101 gctcctggtg ctcgctctcg tccacgcccg ggtcctcgaa gcggatcgag aaggtgcgca
     2161 gctgcgggtg gcccgagtgc ttgaccagcc cggcgatgcc ggaggaatcc aggccgcccg
     2221 acaggtaggc gccgaccggc acgtcggcgc gcagccgcag ccgggtgacg tcgagcagca
     2281 gatcatgcag ctcctcggtg agctcggcga cgctgcccgg caggtactcg ccgtcctcgg
     2341 ggaaggtcca ttcccagtag cgctgccgcc gcagcgtacc gtgctcgacg atgagcatct
     2401 caccgggcga gacttcctcg acgcccttga acaccgacgc cgggctgacc ggcgcccaga
     2461 aggtcatcag ctggttcagc gccgcggcgt ccagctcagg ccggcgccgc atggccggca
     2521 aaagcgcctt cacctcggag gcgaacagca ggcggtttcc ctcgcgcgtg aagtacagcg
     2581 gcaggatgcc cggccggtcg cgcaccagca gcagccggcg gcgcggcgcg tcccacagcg
     2641 cgatggcgaa ctggccgttc atgtggttcg ggaagtcgtc gccgtattcc tcgtagaggt
     2701 gcacgatgac ttcggtgtcg gtgtgggtgt agaaacggtg gccgcggctc tcgaggtccg
     2761 cgcgcagctc gacgtagttg aagatctcgc cgttgaacac cgtccagacg gacttgtcct
     2821 cgttgtggat cggctggtcg ccgccctcga ggtcgatgat gctcagccgc gcatggccca
     2881 ggccgaggcc gggcgcggtg taatagccgg tgccgtccgg cccgcgcgac gccagcgccg
     2941 cgaccatggg ctcgagctcg cggcgcgaca cgtcgccgcc gccgtcgaga tgcaggattc
     3001 cggccaggcc gcacatcagg tcgtcgttcc tctggagctc gtcattcaag tcgtcgttcg
     3061 cgccggcggg ctgatccgca gcccgcgccg cgggtgatcg gccatccagc cggcagagag
     3121 taagccccgc cgcgcggcac gaaccgtgcg ctccggcgca aaaccgccat tctgccatcg
     3181 gcggcccatt cgccgcgaat tccgattgga atcagtctcg gtctgctata ttgcatctat
     3241 aag
//
LOCUS       DAOMPG010000254         3228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12151_length_3228_cov_6.415695, whole genome shotgun sequence.
ACCESSION   DAOMPG010000254 DAOMPG010000000
VERSION     DAOMPG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_3228_cov_6.415695"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLS34_11380"
     CDS             <1..244
                     /locus_tag="PLS34_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP88103.1"
                     /translation="ARLGQASMVSNYTRQLGGVLGVAVVAVFVEWRAAAHTADAAGVF
                     LAYAQAFLLLAVSFALAVAVAVRMRPRAERRSQPAS"
     gene            361..750
                     /locus_tag="PLS34_11385"
     CDS             361..750
                     /locus_tag="PLS34_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88104.1"
                     /translation="MKILKISAPLILAATVLTACASNAFQAAGPARVNDGVLTGANGM
                     TLYTFDKDPEGSGKSVCNGPCATNWPPLLVTTDQSAAPGYSVVMRDDGGKQWAYRGKP
                     LYFWIKDQKPGDRSGDGFNGVWRVAKP"
     gene            756..1256
                     /locus_tag="PLS34_11390"
     CDS             756..1256
                     /locus_tag="PLS34_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP88105.1"
                     /translation="MDSRAILAELPRLRRYARALVGSREAADDLVQDTLERAWSRLAQ
                     WQPGSELRSWLLAIMHNLRIDQMRRPALAVTPLDEEAIELPARATQTDLIELGELETA
                     LSFLPEEQRAVLLLVALEDMSYAQVAATLNIPAGTVMSRLARGRERLRGILDARSKPA
                     QLRVVR"
     gene            1253..2053
                     /locus_tag="PLS34_11395"
     CDS             1253..2053
                     /locus_tag="PLS34_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRP88106.1"
                     /translation="MSSSPISDADLHAFVDGELSEARHAEIAAWLAEHPEDALRVSAY
                     RDQKAGLHRLFDPLLDEPIPDRLRALAVPPAARQDPPQGGPLWRWSLQRMAASVAIAL
                     LGGMAGWLANDHFRPPPVVAHASPLSHQAAIAHVVFSPDVKRPVEITAEHEDQLVTWL
                     SKRLGTTVRPPRLGALGYELIGGRLLPGNSGPVAQFMYHDVSGQRLTLYVSTENTSNR
                     DTAFRFAQEGPVNVFYWIDGKFGYALSAGISKDELARIATAVYDQVQN"
     gene            2081..3091
                     /locus_tag="PLS34_11400"
     CDS             2081..3091
                     /locus_tag="PLS34_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HRP88107.1"
                     /translation="MKKKLNPVRLAAPAIAAGVLLISQHALAASEVSAPEVVEAIEGA
                     FGVTPGERRNHTKGTCALGEFVGTPEAAAYSRSALFSGKPVPVVARFSLSGGNPKAPD
                     TARSGRGMALAFKLPEGQLHHMAMLNTPIFGAATPQTFLDLMQAMRPDPATGKPDPEK
                     LKAFRASHPDSHAQADFLARNNPPASYANSAFWGIHTFKFVDGENRVTLVRWQFVPQD
                     GEKRLSDEELKTAGANFLEQALIERSASGPVRWDMMVSLGQPGDAQTDPTLPWPAERR
                     QIKAGTLTINAAMAQKGAECEPVNFDPMVMADGIQATDDPVLKFRSPAYAVSFGKRLG
                     GM"
BASE COUNT          519 a         1138 c         1089 g          482 t
ORIGIN      
        1 ggcgcggctg ggccaggcgt cgatggtcag caactacacc cgccagctgg gcggtgtgct
       61 cggagtggcg gtggtggcgg tgttcgtgga atggcgggcg gcagcgcaca cggcggatgc
      121 ggcgggcgtc ttcctcgcct acgctcaggc cttcctgctg ctggccgtga gcttcgcact
      181 cgcggtcgcg gtcgccgtac gcatgcgacc gcgggcggag cgccgcagcc agccggcctc
      241 ctgagtccgc atcgagcgcg acttcggcat caatcttctc ggcaagggaa taatcggacg
      301 gtccggctcg tttaccgggt tgtaggccaa ccgttcctga accctcccca ggagacaagc
      361 atgaagatcc tgaagatttc cgccccgctg atcctcgccg ccaccgtgct gacggcgtgc
      421 gccagcaatg ccttccaggc cgccggtccg gcgcgcgtga atgatggcgt gctcaccggc
      481 gccaacggca tgaccctcta taccttcgac aaggaccccg agggcagtgg caagagcgtg
      541 tgcaacggcc cctgcgccac caactggccg cccttgctcg tcaccacgga ccaatcggcc
      601 gcccccggct acagcgtcgt gatgcgcgac gatggcggca agcagtgggc ttacaggggc
      661 aagcccctgt acttctggat caaggaccag aagccgggcg accgctcggg cgacggcttc
      721 aacggggtct ggcgcgtggc gaagccttga gcgtgatgga cagccgcgcc atcctggccg
      781 agctgccccg cctgcgtcgc tatgcacgtg cgctcgtcgg ctcccgcgag gcggccgacg
      841 acctggtcca ggacacgctc gagcgcgcct ggtcgcggct ggcccagtgg cagccgggga
      901 gcgaactgcg atcctggctg ctggccatca tgcacaacct gcggatcgac cagatgcggc
      961 ggccggcctt ggccgtgaca ccgctcgacg aggaggcgat cgagttgccg gcgcgcgcca
     1021 cccagaccga cctcatcgag ctcggcgagc tggaaacggc tctgtccttt ctccccgagg
     1081 agcagcgcgc ggtgttgctg ctggtcgcgc tcgaggacat gagttacgcc caggtcgccg
     1141 cgaccctgaa cattccggca ggtacggtca tgtcccgcct cgcccgcggg cgcgagcgcc
     1201 tgcgcggcat cctcgatgcc cgctccaagc ctgctcaatt gcgagtcgta cgatgagttc
     1261 ctccccgatt tccgatgccg acctgcatgc cttcgtcgac ggcgagctgt cggaggcgcg
     1321 ccatgccgag attgccgcat ggctcgccga acatcccgag gatgccctgc gggtgagcgc
     1381 ctatcgcgac cagaaggcgg gcctgcaccg gctgttcgat ccgctgctcg acgagccgat
     1441 ccccgatcgc ctcagagccc tcgcggtccc gcccgccgcg cggcaggatc cgccgcaagg
     1501 cggcccgctg tggcgctggt cgctacagcg catggcggcg agcgtggcga tcgcgctgct
     1561 cggtggcatg gccgggtggc tggcgaacga tcatttccgg ccgccgccgg tcgtggcgca
     1621 cgcgtcgccg ctttcgcatc aggccgcgat cgcgcacgtg gtcttcagcc cggacgtcaa
     1681 gcgcccggtg gagatcaccg cggaacatga ggatcagctc gtgacctggc tgtcgaaacg
     1741 actcggcacg acggtccggc cgccaaggct gggagcgctc ggctacgagc tgatcggcgg
     1801 ccgcctgctg cccggcaaca gcggcccggt ggcccagttc atgtaccacg atgtgagcgg
     1861 ccagcggctc accctctacg tcagcaccga gaacaccagc aatcgcgaca ccgccttccg
     1921 cttcgcccag gagggaccgg tcaatgtctt ttactggatc gacggcaagt tcggttacgc
     1981 gctctctgca ggcatttcaa aggacgaact ggcgcggatc gccacggcgg tctacgacca
     2041 ggtccagaac tagacgacac cccatacagg aacgatgaag atgaagaaga aattgaaccc
     2101 ggtccggctg gccgcgcccg ccatcgctgc aggcgtgctc ctgatctccc agcatgcgct
     2161 cgccgccagc gaggtgtcgg cgcccgaggt ggtcgaggcg atcgagggag ccttcggcgt
     2221 gacgccgggc gagcggcgca accacaccaa gggcacctgt gccctcggcg agttcgtcgg
     2281 cacgcccgag gcggcggcgt attcgcgctc ggcgctgttc tccggcaagc cggtgccggt
     2341 ggtcgcccgc ttctcgctgt ccggcggcaa ccccaaggcg cccgataccg cccgcagcgg
     2401 acgcggcatg gcgctggcgt tcaagctgcc ggaagggcag ctgcaccaca tggcgatgct
     2461 gaacacgccg atcttcggcg ccgccacgcc gcagaccttc ctcgacctga tgcaggccat
     2521 gcggcccgac cccgccaccg gcaagcccga ccccgagaag ctcaaggcct tccgggccag
     2581 ccaccccgac agccatgcgc aggccgactt cctggcccgc aacaacccgc cggcgagcta
     2641 tgccaacagc gccttctggg gcatccatac cttcaagttc gtggatgggg agaaccgggt
     2701 caccctggtg cgctggcagt tcgttccaca ggacggagag aagcgcctga gcgacgagga
     2761 gctgaagacg gccggggcga acttcctcga gcaggcgctg atcgagcgca gcgccagcgg
     2821 cccggtgcgc tgggacatga tggtcagcct cggtcagccc ggcgatgcgc agaccgaccc
     2881 caccctcccg tggccggccg aacggcggca gatcaaggcc ggaacactga cgatcaacgc
     2941 cgccatggcg cagaagggcg ccgaatgcga accggtcaac ttcgacccga tggtcatggc
     3001 ggacggcatc caggccaccg acgacccggt cctcaagttc cgctcgcccg cctatgccgt
     3061 gtccttcggc aagcggctcg gcggcatgtg atgcgggcgc ggggccgcgt gagcgcaggc
     3121 gtcagcgata ggcgcggatc tgcctgatcg cctccgcggc cgccgcatcg ttggccgggt
     3181 cggtcggcac cgtcagggtg atgcggtcga ccaggccgcc gaagcgcg
//
LOCUS       DAOMPG010000255         3222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12176_length_3222_cov_3.846542, whole genome shotgun sequence.
ACCESSION   DAOMPG010000255 DAOMPG010000000
VERSION     DAOMPG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12176_length_3222_cov_3.846542"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLS34_11405"
     CDS             complement(<1..259)
                     /locus_tag="PLS34_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP88108.1"
                     /translation="MDFRLSERVARIKPSPTMAVTAKAAALKRAGRDVIGLGAGEPDF
                     DTPEHVKAAAIEAIRAGRTKYTAVEGILELREAICKKFRRDN"
     gene            387..2447
                     /gene="uvrB"
                     /locus_tag="PLS34_11410"
     CDS             387..2447
                     /gene="uvrB"
                     /locus_tag="PLS34_11410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRP88109.1"
                     /translation="MSQHPFAESAPRPFELVSDYAPAGDQPEAIAALVQGLEAGLAAQ
                     TLLGVTGSGKTYTIANVIQKLQRPTLVLAHNKTLAAQLYGEFRDYFPGNAVEYFVSYY
                     DYYQPEAYVPSSDTYIEKDASINEHIEQMRLSATKALLSRPDSIIVATVSAIYGLGDP
                     AAYLRMVLHLDRGDRIGQRTLLRRLADMQYTRNEVDLRQGTYRVRGDVIDIFPAESER
                     EAVRVQLFDEEIEALAWFDPLTGELLRSVPRATIFPKTHYVTPRERLLAAIDEIKAEL
                     RARLQVLRSADKLVEAQRLEQRTMFDLEMIQELGYCQGIENYSRYLSGRAPGEPPPCL
                     FDYLPKDALLVVDESHVTIPQLGGMYKGDRSRKETLVEFGFRLPSALDNRPLRFDEFE
                     ALSPQAIYVSATPREYELERSGQVIEQVVRPTGLVDPEVEVRPVTHQVDDVLSEIGAR
                     VAGNERVLITTLTKRMAEDLTDYLAEHQVRVRYLHSDIDTAERTEIIRDLRRGEFDVL
                     VGINLLREGLDMPEVSLVAILDADKEGFLRSEGSLIQTIGRAARNVHGKAILYADNVT
                     GSMRRAIDETNRRRARQIDFNAAHGITPQTIVKRVADVMEGAREEGGSRGRDEAVRRK
                     VAEPRLAYERLSPEEVMKRIRKLEGEMYRHARELEFEQAAELRDEIERLRHEGLGLPS
                     DLAG"
     gene            2506..2580
                     /locus_tag="PLS34_11415"
     tRNA            2506..2580
                     /locus_tag="PLS34_11415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2538..2540,aa:Val,seq:gac)
     gene            2664..>3222
                     /locus_tag="PLS34_11420"
     CDS             2664..>3222
                     /locus_tag="PLS34_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRP88110.1"
                     /translation="MPLITLPDGSQRSFEQPVTVAELAADIGPGLAKAAVAGRVDGRL
                     VDTSHRIETDAEVAIVTGRDADGLEIIRHSTAHLLAQAVKQLFPGAQVTIGPVIEDGF
                     YYDFAFERAFTPEDLEAIEKRMAELAEADQPVHREVMARDDAVAFFRSIGEEYKARII
                     EAIPANEVISLYGQGDFKDLCRGPHV"
BASE COUNT          553 a         1086 c         1093 g          490 t
ORIGIN      
        1 ggttgtcgcg gcggaacttc ttgcagatgg cctcgcgcag ttcgaggatg ccctcgaccg
       61 cggtgtactt ggtccgtccg gcgcgaatcg cctcgatcgc cgccgccttg acgtgctcgg
      121 gcgtgtcgaa atccggctcg ccggcgccga ggccgatgac gtcgcgcccg gcgcgcttga
      181 gcgcggcggc cttggcagtg acggccatgg tgggcgaagg cttgatgcgg gcgacccgct
      241 cggaaaggcg aaagtccacg agattcccct ggtgcataat gaagggctgg cgggcgagcg
      301 cccacgaggc tgagggggcc ccggggcgaa ttcgcccgcc atgttgaaat gaaagccagt
      361 cacagtccag taggtattgt cgcgaaatga gccagcaccc gtttgccgag tccgccccca
      421 ggcccttcga gctggtctcg gactatgcac ctgccggcga ccagccagag gcgatcgcgg
      481 cgctcgtgca gggcctcgag gccgggctcg cggcgcagac gctgctcggg gtcaccggct
      541 cgggcaagac ctacacgatc gccaacgtca tccagaagct gcagcggccg acactggtgc
      601 tggctcacaa caagacactg gccgcgcagc tctacggcga gttccgcgac tactttcccg
      661 gcaatgcggt cgagtatttc gtctcctact acgactatta ccagcccgag gcctacgtgc
      721 cgtcctccga cacctacatc gagaaggacg cctccatcaa cgagcacatc gagcagatgc
      781 ggctgtcggc gaccaaggcg ctgctgtcgc gacccgacag catcatcgtg gcgacggtct
      841 cggcgatcta cggcctgggc gacccggcgg cctacctgcg catggtgctg cacctggatc
      901 gcggcgaccg gatcggccag cgcaccctgc tgcggcggct ggcggacatg cagtacacgc
      961 gcaacgaggt cgacctgcgg cagggcacct accgcgtacg cggcgacgtg atcgacattt
     1021 tcccggccga gtcggagcgc gaagcggtcc gggtgcagct gttcgacgag gaaatcgagg
     1081 cgctggcgtg gttcgatccc ctgaccggcg aactgctgcg cagcgtgccg cgggcgacca
     1141 tcttccccaa gactcactac gtgacgccgc gtgagcggct gctggcggcg atcgacgaga
     1201 tcaaggccga actgcgcgcg cgcctgcagg ttctgcgctc cgccgacaag ctggtggagg
     1261 cccagcgcct cgagcagcgc accatgttcg acctcgagat gatccaggag ctgggctact
     1321 gccaggggat cgagaactat tcccgctacc tgtcggggcg ggcgcccggc gagccgccgc
     1381 cctgcctgtt cgactacctg ccgaaagacg cgctgctggt ggtggacgag tcgcatgtca
     1441 ccattccgca gctcggcggc atgtacaagg gcgaccgcag ccgcaaggaa acgttggtcg
     1501 aattcggctt ccgcctgccg tcggcgctcg acaaccggcc gttgcgcttc gacgagttcg
     1561 aagcgctgtc gccgcaggcc atctacgtat ccgcgacgcc gcgcgagtac gagctggagc
     1621 gctcgggcca ggtcatcgaa caggtggtgc ggcccaccgg gctggtcgac ccggaggtcg
     1681 aggtgcggcc ggtgacccac caggtggacg acgtgctgtc cgagatcggc gcccgggtcg
     1741 ccggcaacga gcgcgtgctg atcaccacgc tgaccaagcg catggccgag gatttgaccg
     1801 actacctggc cgagcaccag gtgcgggtgc gttatctgca ctcggacatc gacacggcag
     1861 agcgcaccga gatcatccgc gacctgcgcc gcggcgagtt cgacgtgctg gtcggcatca
     1921 acctgctgcg cgaagggctc gacatgccgg aggtgtcgct ggtcgccatc ctcgacgccg
     1981 ataaggaagg cttcctgcgt tccgagggct cgctgatcca gaccatcggc cgggccgcgc
     2041 gcaacgtgca cggcaaggcg atcctgtacg ccgacaacgt caccggctcg atgcgccgcg
     2101 cgatcgacga gaccaaccgg cgccgcgcca ggcagatcga tttcaatgcg gcgcacggca
     2161 tcacgccaca gaccatcgtc aagcgggtcg ccgacgtgat ggagggtgcc cgcgaggagg
     2221 gcgggtcgcg cggccgcgac gaggcggtgc gccgcaaggt ggccgagccg cggctggctt
     2281 acgagcggct cagcccggag gaagtgatga agcgcatccg caagctggaa ggcgagatgt
     2341 atcgccatgc ccgcgagctc gagttcgagc aagccgccga gctgcgcgac gagatcgaac
     2401 gcctgcgcca cgagggcctc ggcctgccct ccgacctggc cgggtgaggg gcaatcaagg
     2461 cttgcccggc ggggggttcg ccggtatcct tcccgccctt gaatcaggcg cgtagctcag
     2521 tggtagagca ctacgttgac atcgtagggg tcggcggttc aaacccgccc gcgcctacca
     2581 aatccgagac agccacgccc cggtgcggcc cgccaaaacg gtccttgccg gtgcgtggcg
     2641 tccgattctg agcaacaaac cccatgcccc taatcacgct tcctgacggc agtcagcgca
     2701 gcttcgagca gcctgtcacg gtcgccgagc tcgctgcgga catcggtccc ggcctcgcga
     2761 aggccgcagt ggccgggcgc gtcgatggcc gcctggtcga cacctcgcac cgcatcgaga
     2821 ccgatgcgga agtcgcgatc gtcaccgggc gggatgcgga cggcctcgaa atcatccgcc
     2881 actcgacggc gcacctgctt gcgcaggccg tgaaacagct gtttcccggc gcccaggtga
     2941 ccatcggacc ggtcatcgag gacggtttct actacgactt cgccttcgag cgcgccttca
     3001 cgccggagga cctcgaggcc atcgagaagc gcatggcgga gctggccgag gccgaccagc
     3061 cggtgcatcg cgaggtcatg gcgcgcgacg acgccgtcgc gttcttccgc agcatcggcg
     3121 aggagtacaa ggcccgcatc atcgaggcca ttccggcgaa cgaggtcatc agcctgtacg
     3181 gccagggcga cttcaaggat ctttgccgcg gtccgcacgt gc
//
LOCUS       DAOMPG010000256         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12209_length_3215_cov_3.342405, whole genome shotgun sequence.
ACCESSION   DAOMPG010000256 DAOMPG010000000
VERSION     DAOMPG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12209_length_3215_cov_3.342405"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..3089)
                     /locus_tag="PLS34_11425"
                     /pseudo
     CDS             complement(1..3089)
                     /locus_tag="PLS34_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086211.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          504 a         1145 c         1115 g          451 t
ORIGIN      
        1 gggcctgctc ggacgccctg agcttctcca gcttcttgct cacgtgcccc ggcgactggg
       61 tgttgcgggc aatcaccgag taggccgccg gcaccacgaa cagcgtcagg aaagtcgtca
      121 gggccatgcc gtagaccacc acgatgccca ccgcctggcg gctctccgag ccggcgccgc
      181 tggccagcag caggggcagg gcgccgatgg ccgtgcaggc gctggtcatg agcaccggcc
      241 gcaagcggat tgccgccgcc tcgaccaccg cctggtcgaa cgccatgccg cggtcgcgca
      301 gctggttggc gaactcgacg atcagcacgc cgttcttggc cgccaggccg accagcatga
      361 tcaggccgat ctggctgtag acgttgatcg agccgccgta ggcccacaag ccgagcagcg
      421 cgccggtgat cgccagcggc acggtgaaca tgatcaccag cggatggcgg aagctctcga
      481 actgcgccgc cagcaccagg aacaccacga tcagcgccat cacgaaggtg acgtacagcg
      541 agccgccgga ttccttgaac tcgcgtgact cgccgtccca gccgatgcgc acttcgggcg
      601 gcaggatcgc gcgcgccagc gcctcgaact cgtccagcgc ctggccgagc gaggcgccgg
      661 gctcgaggcc cgcggtgacc gtgatggagc gcagccggtc gaagcgcctg agctccgccg
      721 ggccggcccg ttcggcaatg gacaccaggt tgctgagcgg caccagccgg ccgctggtgg
      781 ccgagcgcac gtagatgttc gacaggtcgc tgggcgtggc gcggccctcg gcctggccct
      841 gcaggatgac gttgtactcg cggtcgcggt cgatgaaggt cgtgacctgg cgcgagccga
      901 gcatggtctc gagcgtgcgg ccgaccgtct ccagcgagac gccgagctcc gcggcgcggt
      961 tgcggtcgac caccacgcgc atctgcggct gccgctcctc gtagttggaa tcgaggccgc
     1021 gcatgccggc gagcccctcc gcggcggcca tcaggtcgtc gcgccaggcg gcgagctgcg
     1081 cgtactcggc gccgccgagc accatctgca ccgggcgatc cgcgccgcgc acgcccaggc
     1141 cggccggcgt gatcacccgc gcctggacgc cggggaagtc ggccagctcc ttgcgcacct
     1201 cgtcggcaat ctgctcgacc gtgcgctcgc gcttcgccca gtcctccagc agcacgatgg
     1261 cgcgcgcgct gtccacgccg ccgccgccgc cccagccgcc cggcacgcgc agcagcacgc
     1321 ggcgcacctc gccactctcg acgtagcgca tcaggatcgc ttccatgcga cgcgcatagg
     1381 cctcggtgta ctccaggctg gcgccgggcg gggccgtgat cagcgtgaag aagatgccgc
     1441 ggtcttcgcg cggcgcgtac tcggtcggca gcacgcggaa caacccggcg gccgccacgg
     1501 tgaccagcag cgcgacggcc attaccggcc acggccgcgg cacgaccgcg ccgagcatgc
     1561 gccggtacac gtagcccagt cgcttgaagc cgctctcgat cttcgtcgtc agccagccgc
     1621 ggcgctcgtt ggccttgaac agcttggagc tgagcatcgg aatcagcgtc agcgcgacga
     1681 ggctggagat gccgaccgcc gccgccaccg ccacgccgaa ctcgcggaac agccggccga
     1741 cgttgccctg gatgaaggag atcggcagga acaccgacac cagcaccagc gtggtcgcca
     1801 ccacggcgaa gccgatctcg cggctgccgt tgaccgccgc cagcagcggc ggctcgccag
     1861 atgttctcca gcaccacgat ggcgtcgtcg accaccaggc cgatggccag caccaggccg
     1921 agcaggatga gcacgttgat ggaatagccg aacagcgcca tcaccgagaa ggtggcgatg
     1981 acgcagatgg ggatggtcac cgccggcacc agcgtcgcgc gcaggttgcc gaggaacagg
     2041 tagatgacca gcagcaccag cgacagcgcg aagccgagcg ccttgaccac ctcgagcatg
     2101 gaggcctcga tgaaatcggc gcggtcgaag ttgatgccga tctccacgcc ctcgggcagc
     2161 gagggcatga cgtccaccat ggcggcgcgt atgcccttgg agacctcgac ggtgttggcc
     2221 ttggactgct gctcgatgcc gaggctgatg gcgggctcct ggttggcgcg cgccacgctg
     2281 cgttcgtcct cggcgccgat gcgcacctcg gcgacgtctg cgaggcgcac gaagttgccg
     2341 tcgccgccgc gctggatgac cagcgccgca aaatcctccg gccgcgtcag cccggtgcgg
     2401 gtgcgcagcg tgaactcgcg ctgcgcggac tcgaggcgcc cggccggcaa ctcgacgttc
     2461 tcgcgcctga gcgctgcggt gatgtcctcg gaggtcacgc cgcgggccgc catcgcctcg
     2521 cggtccagcc acacgcgcat ggcgtagcgg cgcgcgccgc cgaggcgcac gcgcgccacg
     2581 ccctcgacca ccgagaagcg gtccaccagc acgcgctcgg cgatgtcggt cagttccagc
     2641 tcgctgtgcc ggtcgctggt gaaggtcagc cacatcaccg gctgcgtgtc actgtccgac
     2701 ttggcgatct gcggcgggtc ggcctcggcc ggcagcgacg agaccacgcg cccgacgcga
     2761 tcccgcacgt cgttggcggc ttcatccacg ttgcggttga gcgagaactc gatggtgacg
     2821 ctggagcgct catcgcggct ggtcgaggtc agcttggtga tgccctcgag gccggcgacc
     2881 tggtcctcga tgacctgggt gacgcgcgtc tcgatgacct cggccgaggc gccgcgatag
     2941 gtggtgtcga tggagacgat cggcgggtcg atgtccgggt attcgcgcac cgccaggttc
     3001 atggccgagg ccaggccgat gagcaccagg aacatcgaca gcacggcggc gaacacgggc
     3061 cggcggacgg acagctcgga gattttcatc ggttaccgca ttccccggtc ggcaggtcga
     3121 tgtgcaggtg catcgagggt tgcctcgctt cagccgcggc caagggtcgc caccggctcg
     3181 gcggccgtga cccgcccgcc gttggcgacc ttctg
//
LOCUS       DAOMPG010000257         3204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12259_length_3204_cov_4.854875, whole genome shotgun sequence.
ACCESSION   DAOMPG010000257 DAOMPG010000000
VERSION     DAOMPG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_3204_cov_4.854875"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLS34_11430"
     CDS             complement(<1..835)
                     /locus_tag="PLS34_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRP88111.1"
                     /translation="MNDLAKNIVLWIVIAVVLLTVFNSFGTAPRQVQDIPYSTFLQRV
                     DDGSVSEVVFEGDKIVGAFSSGERFTTYSPETDNRALIGELKNAGVNFRSSPPQRQSF
                     LMQLFISSFPILLLIAVWVYFMRQMQGGGGGRGAMSFGKSRARLLGEDQVNVTFADVA
                     GVEEAKEEVSEIVDFLKDPAKFQRLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQ"
     gene            complement(889..1515)
                     /gene="rlmE"
                     /locus_tag="PLS34_11435"
     CDS             complement(889..1515)
                     /gene="rlmE"
                     /locus_tag="PLS34_11435"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HRP88112.1"
                     /translation="MSGRTRRGKRWMDEHVEDEFVRRAKQEGWRSRAVYKLMEIQEKD
                     RVLKPNMTVVDLGAAPGAWSQFAAQAVGRRGRVIALDILPMDPLPGVQFIEGDFREPA
                     TLAALEAALGDTAPGLVLSDMAPNISGMATVDQPRAMYLAELALDFARARLAPGGALV
                     TKVFQGEGSDALVREARKDFGTVRMRKPKASRDRSREFYLVAGNYRVM"
     gene            1642..1920
                     /gene="yhbY"
                     /locus_tag="PLS34_11440"
     CDS             1642..1920
                     /gene="yhbY"
                     /locus_tag="PLS34_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRP88113.1"
                     /translation="MSLNEKQKKQFRKQGHALEPVILTGGGGLSPALLAELESALAHH
                     ELLKVRVRAADRDSRDQMIEAMCRHTGAELVQRIGHVALLWRPNPDKA"
     gene            complement(1973..2449)
                     /gene="greA"
                     /locus_tag="PLS34_11445"
     CDS             complement(1973..2449)
                     /gene="greA"
                     /locus_tag="PLS34_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRP88114.1"
                     /translation="MKKVPMTAQGAARLREELTRLKNVERPRISQAIGEARAHGDLKE
                     NAEYHAAKEQQGLVEARIRDIEHKLSHAEIIDVTALNAGGRVVFGATVELFCATEERE
                     LRYQIVGEEEADLREGRISISSPIARALIGKGEGDEITVRAPSGDRVYEILQVLYL"
     gene            complement(2446..>3204)
                     /gene="carB"
                     /locus_tag="PLS34_11450"
     CDS             complement(2446..>3204)
                     /gene="carB"
                     /locus_tag="PLS34_11450"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365473.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HRP88115.1"
                     /translation="KGLMNTQFAIQNGQIFLLEVNPRASRTVPFVSKATGRPLAKIAA
                     LCMAGVSLKEQGVSGERRPDYLSVKASVFPFAKFLGADPILGPEMKSTGEVMGTGRFF
                     GEAYAKAQLAAGVLLPRGGTALLSVRDEDKTGAVELARILVDRGFQLVATAGTAQVVQ
                     EAGVPCRSVFKVREGRPHIVDMIKNGEISLIVNTTANRQAIRESHSIRREAVHRKVTY
                     YTTIAAAKATCVALDHLDATDVNRLQDLHTEISP"
BASE COUNT          513 a         1137 c         1002 g          552 t
ORIGIN      
        1 tctgctcgcg ctcgtcgtga ccgccgccca ggccggcgcc gcgatggcgg ccgaccgcgt
       61 cgatctcgtc gatgaagatg atgcagggcg cgtgcttctt ggcctgctcg aacatgtcgc
      121 gcacgcgcga ggcgccgacg ccgacgaaca tctcgacgaa atcggaaccg gaaatggtga
      181 agaacggcac cttcgcctcg ccggcgatgg cgcgcgccag cagcgtcttg ccggtgccgg
      241 gcgagccgac catcagcacg cccttgggaa tcttgccgcc aaggcgctgg aacttggccg
      301 ggtccttgag gaagtcgacg atctcgctga cttcttcctt ggcctcctcg acgcccgcga
      361 cgtccgcgaa ggtgacgttg acctggtcct cgccgagcag ccgcgcccgg ctcttgccga
      421 aggacatggc cccccggccg ccgccgccgc cctgcatctg gcgcatgaag tacacccaga
      481 ccgcgatgag cagcaggatc gggaatgaag aaatgaacag ctgcatgagg aaggactgcc
      541 gctgcggcgg gctggagcgg aaattcacgc ccgcattttt cagctcgccg atcagcgcgc
      601 ggttgtccgt ctccgggctg taggtggtga agcgctcgcc gctgctgaag gcaccgacga
      661 tcttgtcgcc ctcgaacacc acttcgctga ccgaaccgtc atcgacccgc tgcaggaagg
      721 tggaatacgg gatgtcctgg acctggcgcg gcgccgtccc gaaactgttg aagaccgtca
      781 acagcacgac ggcgatcacg atccacaaca cgatgttctt ggctagatcg ttcaaagacc
      841 ctgctcctca gctctcggcc cgggcatgcc gcccgcctcg tttagacact acatgacccg
      901 gtagtttcct gccaccaggt aaaattcgcg gctccggtcc ctggacgcct tcggcttgcg
      961 catgcgcacc gtgccgaaat ccttgcgggc ctcccgcacc agggcgtcgc tgccttcccc
     1021 ctggaacacc ttcgtcacca gcgccccgcc cggagccagc cgggcacgcg caaaatccag
     1081 cgccaattcc gccaggtaca tggcgcgggg ctggtccacc gtcgccatac cgctgatatt
     1141 gggtgccatg tcgctcaata caagccccgg cgccgtgtcg ccaagcgccg cttcgagtgc
     1201 cgccagcgtc gcgggctcgc ggaaatcgcc ctcgatgaac tgcacccccg gcaggggatc
     1261 catgggcagg atgtccagcg cgatcacccg gccgcgccgc cctaccgcct gcgcggcgaa
     1321 ctggctccag gcccccggcg ccgcgcccag gtcgaccacc gtcatgttcg gcttcagcac
     1381 ccggtccttc tcctggatct ccatgagctt gtagacggcc cgggagcgcc agccctcctg
     1441 tttcgcccgc cgcacgaact cgtcctcgac gtgttcgtcc atccagcgct tgccacgacg
     1501 tgttcgtccg ctcattgccc cccggattcc tcgtcgccgg ccgtgcttgc ggccccggcc
     1561 tgatagacta gcggcccgcg cgccgcgggg tgtgccgccg ggccaggcct gacctcataa
     1621 attgccggaa gaacatcttt catgtcattg aatgaaaaac agaaaaaaca atttcgaaag
     1681 caaggtcatg cactcgaacc ggtgatcctc accggcggcg gcggcctgag cccggcgctg
     1741 ctcgcggaac tcgagtcggc gctcgcccat cacgagctct tgaaggtgcg ggtccgggcc
     1801 gcagaccgtg acagccgcga ccagatgatc gaggcgatgt gccggcacac cggcgccgag
     1861 ctggtgcagc gcatcgggca cgtcgcgctg ctgtggcgcc ccaaccccga caaggcctga
     1921 tgaatttggg gacattcccc aattccgccg gaattgggga atgtccccaa attcaaagat
     1981 acaggacctg caggatttcg tagacgcgat cgccgctcgg cgcgcgtacg gtaatctcgt
     2041 cgccctcgcc cttgccgatc agcgcgcgcg cgatcggcga ggagatcgaa atccggccct
     2101 cgcgcaggtc ggcctcttcc tcgccgacga tctggtagcg cagttcgcgc tcctcggtgg
     2161 cgcagaacag ctcgacggtg gcgccgaaca ctacccggcc gccggcattg agcgccgtca
     2221 cgtcgatgat ctcggcatgc gacagcttgt gctcgatgtc gcggatgcgc gcttccacca
     2281 gcccttgctg ctccttggcg gcgtggtatt cggcgttctc cttcaggtcg ccgtgcgcgc
     2341 gcgcctcgcc gatcgcctgg ctgatgcgcg gccgctcgac gttcttcagc cgcgtcaact
     2401 cctcgcgcag ccgcgccgcg ccctgggcgg tcatgggcac cttcttcatg gcgagatctc
     2461 cgtgtgcagg tcctgcaacc ggttgacgtc cgtcgcgtcg agatgatcga gcgccacgca
     2521 ggtcgccttg gcggccgcga tggtggtgta gtaggtcacc ttgcggtgca ccgcttcgcg
     2581 ccggatcgaa tgcgattcgc ggatcgcctg gcggttggcg gtggtgttga cgatcaggct
     2641 gatttcgccg ttcttgatca tgtccacgat gtgcggccgg ccttcgcgta ccttgaatac
     2701 cgagcggcag ggcacgcccg cctcctgcac gacctgcgcc gtacccgcgg tcgccaccag
     2761 ctggaagccg cggtccacca ggatgcgcgc cagctcgacc gccccggtct tgtcctcgtc
     2821 acgcacgctg agcagcgccg tgccgccacg cggcagcagc acgcccgcgg ccagctgcgc
     2881 cttggcgtag gcctcgccga agaagcgccc ggtgcccatc acctcgccgg tcgacttcat
     2941 ctccggcccg agaatcgggt cggcgccgag gaacttggcg aaggggaaca ccgaggcctt
     3001 caccgacagg taatcgggcc ggcgctcgcc gctcacgccc tgctccttga ggctcacccc
     3061 ggccatgcac agcgccgcga tcttggccag cgggcggccg gtcgccttgg agacgaacgg
     3121 cacggtgcgg gaagcgcgcg gattgacctc cagcaggaag atctggccgt tctggatggc
     3181 gaactgggtg ttcatcagcc cctt
//
LOCUS       DAOMPG010000258         3180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12355_length_3180_cov_3.165120, whole genome shotgun sequence.
ACCESSION   DAOMPG010000258 DAOMPG010000000
VERSION     DAOMPG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12355_length_3180_cov_3.165120"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLS34_11455"
     CDS             complement(<1..497)
                     /locus_tag="PLS34_11455"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HRP88116.1"
                     /translation="MARSAGITLVELLTVIAIIAVLAGLAIPALDGLLLNARRAAAMD
                     GLVRAAWFARTEALRRGRPVVLCAAAGSDRCLGDPDGWTGGWVITPLDAPGEVLRRGA
                     GTADPRAQLLSNRGVFSFEPHDRRSTNGTLAWCDARGAQAARALIIAPTGRPRLERGA
                     GSLPCP"
     gene            616..>1422
                     /locus_tag="PLS34_11460"
     CDS             616..>1422
                     /locus_tag="PLS34_11460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP88117.1"
                     /translation="MKQSADLIVIGGGALGMISARRFAQSGRKVVLLERQRVGRGTSR
                     AGGGIMSPLEPWRAAQPVADLAALSLPLLPGLTEELHRDTGIDPLYRSTGAIYLDCEA
                     LDTALAYARDRGLRAELLDEAELAAIAPAAARRAGPSLLLPDVAQLRNPRFLDALAAD
                     LRRRGVELLEEAGDARLEAAGAGLVVHAGPHGRLQATDVVVAAGAWSAGLLAPLGVQL
                     PVIPVRGQILWYLLPRPVLSQILMHRGRYVIPRHDGVVLVGSTLEGVGFED"
     assembly_gap    1423..1443
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1444..1754
                     /locus_tag="PLS34_11465"
     CDS             <1444..1754
                     /locus_tag="PLS34_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP88118.1"
                     /translation="AAELHAAAADMVPLLGSLPVQGQWAGLRPAAPDGIPMIGPVPGI
                     PGLWLNTGHFRNGVNLAPGSAALLEALVSGRTPPLDPRPFDPAARMAPGASATYNPSP
                     "
     gene            1771..2178
                     /locus_tag="PLS34_11470"
     CDS             1771..2178
                     /locus_tag="PLS34_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRP88119.1"
                     /translation="MITQESVAKRLSSHGAKPTTQRLKLAEVVFARPQHLSAEQVLQV
                     ARTRGIRVSKATVYNTLNLFVDCGLLRELVVDRDRVYYDSTVHAHHHFYNVDTGEMID
                     IPAEAVSFAGVPAAPAGTEQDGVEVIIRVRNRS"
     gene            2248..2323
                     /locus_tag="PLS34_11475"
     tRNA            2248..2323
                     /locus_tag="PLS34_11475"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2281..2283,aa:Thr,seq:cgt)
     gene            complement(2574..2978)
                     /locus_tag="PLS34_11480"
     CDS             complement(2574..2978)
                     /locus_tag="PLS34_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP88120.1"
                     /translation="MRGVALLVGMLALCAGCKPGGPSLGEQIYRDGVGASGRLQYSQG
                     PEWLRFAGPACLVCHGERGQGLTVQAGEVTGAAPAISWTALEQRGYDEASFRRALLTG
                     VDPHGREFIDYMPRWQMSEAETDALIEYLRGL"
     gene            complement(2980..>3180)
                     /locus_tag="PLS34_11485"
     CDS             complement(2980..>3180)
                     /locus_tag="PLS34_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88121.1"
                     /translation="PASFLELDARFHYLAGMLEHVAEARKPELVGYYLGAMTETCVAC
                     HARHAQAKFPALADAAPHDHQH"
BASE COUNT          441 a         1108 c         1128 g          482 t
ORIGIN      
        1 ggacagggca atgagcccgc accgcgctcg agacgcggac ggccggtcgg ggcgatgatc
       61 aaggcacggg cggcctgcgc gccgcgcgca tcgcaccagg cgagcgtgcc gttggtgctg
      121 cgccggtcgt gcggctcgaa gctgaacaca ccgcggttcg acagcaactg cgcgcgcgga
      181 tccgccgtgc ccgcaccccg gcgcagcacc tcgccggggg cgtccaacgg cgtgatcacc
      241 cagccaccgg tccacccgtc cggatcgccg aggcagcggt cgctgccggc cgccgcgcac
      301 agcaccacgg ggcggccgcg ccgcaacgcc tcggtgcggg cgaaccacgc ggcacgcacc
      361 agtccgtcca tggccgccgc gcggcgcgca ttgagcagca ggccgtccag cgcggggatc
      421 gcgagtcccg cgagcaccgc gatgatcgcg attaccgtga gcagttcgac cagcgtgatg
      481 cctgcgctgc gtgccatggc gtcctccctg gcaggcagct tgccgccgcc gtccgcgacc
      541 cgggagtcac ggaaactgcg gtttccctgc ggatatggga caggctatgg tgcccgtcga
      601 gaaaggagtg ttcgcttgaa gcaatcagcc gacctcatcg tcatcggcgg cggcgcgctg
      661 gggatgatca gcgcccgccg cttcgcgcag tccggccgca aggtggtgct gctggagcgg
      721 caacgcgtcg ggcgcggcac gtcgcgggcc ggcggcggca tcatgtcacc gctcgagccc
      781 tggcgcgcgg cgcagccggt ggccgacctg gcggcgctgt cgctgcccct gttgcccggg
      841 ctgacggagg aactgcaccg tgataccggc atcgacccgc tgtatcggag caccggcgcg
      901 atctatctcg actgcgaggc gctagatacc gcgctggctt acgccaggga cagggggctg
      961 cgggccgagt tgctggacga ggccgagctg gcggcgatcg cgccggcggc ggcgcggcgc
     1021 gccgggccgt cgctgctgtt gccggacgtc gcgcagttgc gcaatccacg cttcctggac
     1081 gcgctggccg cggacctccg gcgccggggc gtcgagctgc tggaggaggc gggtgacgcc
     1141 aggctggaag ccgcgggggc ggggctggtg gtgcatgccg gcccgcacgg ccggctgcag
     1201 gcgaccgacg tggtggtggc cgctggcgcc tggagcgccg gcctgctggc gccactgggc
     1261 gtgcagctgc cggtgatccc ggtgcgtggc cagatcctct ggtacctgct gccgcggccc
     1321 gtgctcagcc aaattctcat gcaccggggc cgctacgtga taccgcggca cgacggcgtg
     1381 gtgctggtcg gcagcacgct ggagggggtc ggcttcgagg atnnnnnnnn nnnnnnnnnn
     1441 nnncggccgc agaactgcac gcagctgccg cggacatggt cccactgctg gggtcgctgc
     1501 ccgtgcaggg ccagtgggcc ggcctgcggc ccgctgcgcc agacgggatt cccatgatcg
     1561 ggccggtgcc cggcatcccc gggctgtggc tcaacacggg gcatttccga aacggggtca
     1621 acctggcgcc ggggtcggcg gccctgctcg aggccctggt gagtggccgg actccgccgc
     1681 tggacccgcg gcccttcgac ccggccgcga ggatggcgcc gggcgccagc gcgacatata
     1741 atccaagtcc ctgataaggt tcagatggtc atgatcaccc aggaaagcgt cgccaagcga
     1801 ctgtccagtc acggcgcaaa acccaccacc cagcggctca agctggccga ggtggtgttc
     1861 gcgcgtccgc agcacctctc cgccgagcag gtgctccagg tggcgcgcac gcgtggcatc
     1921 cgggtgtcca aggcgaccgt gtacaacacc ctgaacctgt tcgtggactg cggcctgctg
     1981 cgcgagctcg tggtggaccg cgaccgggtc tattacgact ccacggtgca tgcgcaccat
     2041 catttctaca acgtcgatac cggcgagatg atcgacatcc cggccgaggc cgtgtccttc
     2101 gcgggcgtcc cggcggcgcc ggccggcacg gagcaggacg gggtggaagt catcatccgg
     2161 gtgcgcaacc gcagctgagc cgctcgtcgg cgttgccgct gccgcggcca tctcctatac
     2221 tgcgcggcct ttccgaggct ccttcgtgcc ggggtagctc agtcggcaga gcagctgatt
     2281 cgtaatcagc aggtcggtgg ttcgaatcca ctctccggca ccatcaaatc gctgcggacg
     2341 ggcgcaccac gcccggcgcc tcccgcgcca ccccatgcta tctccaggcc cgcttacctc
     2401 gccttcttgg cacgcttctc ggcgcgcttt tcctccagcg tccgggtcgg tttcttcttg
     2461 gtttctttct ttggctcttt cgacttgctc atcgtggcgc tcccgtccat gtgcgtggat
     2521 gctcaaccaa ccatgggaag gtagaccgcg gcgcgaggtc gcgcaatgcc cggtcagagc
     2581 ccgcgcaggt attcgatcag cgcgtctgtt tcggcctcgc tcatctgcca gcgcggcatg
     2641 tagtcgatga actcgcggcc gtgcggatcg acgccggtca gcagcgcgcg gcggaacgac
     2701 gcctcgtcgt aaccgcgctg ctcgagtgcc gtccaggaga tcgcaggggc tgcgcctgtg
     2761 acctcgccgg cctgcactgt caggccctgg ccgcgctcgc cgtggcagac caggcaggcg
     2821 ggcccggcaa agcgcagcca ctccggtccc tggctgtatt gcaggcggcc cgaggcgccc
     2881 acgccgtccc ggtagatctg ctcgcccagc gaagggccgc ccggcttgca gccggcgcac
     2941 agcgcgagca tcccgacgag cagcgctacg ccgcgcatct cagtgctggt gatcgtgcgg
     3001 cgcggcgtcc gccagcgccg ggaacttggc ctgcgcgtgg cgcgcatggc aggcgacgca
     3061 ggtctcggtc atggcgccga ggtagtagcc gacgagctca ggcttcctgg cctcggcgac
     3121 gtgctcgagc atcccggcga ggtaatgaaa gcgcgcatcc agctccagga acgaggcggg
//
LOCUS       DAOMPG010000259         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12368_length_3177_cov_5.737668, whole genome shotgun sequence.
ACCESSION   DAOMPG010000259 DAOMPG010000000
VERSION     DAOMPG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12368_length_3177_cov_5.737668"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1145)
                     /gene="pspF"
                     /locus_tag="PLS34_11490"
     CDS             complement(96..1145)
                     /gene="pspF"
                     /locus_tag="PLS34_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017787142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HRP88122.1"
                     /translation="MDVDTSSPMLGASPAFGAMLEQVSRLAPLDRPALIIGERGTGKE
                     LVAARLHYLSPRWEQPLVKLNCAALTESLLESELFGHEAGAFTGATRRHVGRFERADG
                     GSLFLDELGTLPARMQEKILRVIEYGEFERVGGSETLRADVRIVGATNENLPALARAG
                     RFRADLLDRLAFDVIHVPPLRARPEDILELAQHFAVGFTAELKRPFFPGFSESAVAAL
                     LAWPWPGNVRELKNAVERSIYRAADPERLVDEIHFDPFAHDHGDEPAALDGDAQAAAG
                     TGNPTPQAPALPLDFRGAVAGFEQRLLTRALAAADGKRRVAAESLGLSYDQLRGQLRK
                     HGIGGAGRPGRPENR"
     gene            1312..1989
                     /gene="pspA"
                     /locus_tag="PLS34_11495"
     CDS             1312..1989
                     /gene="pspA"
                     /locus_tag="PLS34_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HRP88123.1"
                     /translation="MGIFSRFSDIINSNINALLEKAEDPEKMVRLIIQEMEDTLVEVR
                     SAAARAIADRKQLDRRLAEARREGDDWSRKAELAVRKGRDDLARAALSEKARAEQHVA
                     SLEEQGAQVQEALARLNDDMARLQEKLADAKTRQKAMVMRHRTAGHRLEMRRQIHESK
                     IDDALVRFEQFERKLEDVEGRVESYDLGQRKDLGREFASLESEESVEAELAALKRRVG
                     GESRGEA"
     gene            2025..2252
                     /gene="pspB"
                     /locus_tag="PLS34_11500"
     CDS             2025..2252
                     /gene="pspB"
                     /locus_tag="PLS34_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016710250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HRP88124.1"
                     /translation="MSEFFGVVMIIVAAVVLPLVVILHYVTKWKSSRTLSGDEQRMLE
                     ELWQDAQKMESRINALETILDDQVPDWRRRT"
     gene            2249..2530
                     /locus_tag="PLS34_11505"
     CDS             2249..2530
                     /locus_tag="PLS34_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRP88125.1"
                     /translation="MNRRKHAPATAIFYRDPANGMIMGVCAGLSDQFGFRLVPLRIIA
                     VAALVLFTVPTLLAYLVAGVLLPGKPLTYYGHRDERELWGRRRTERMQA"
     gene            2527..2922
                     /gene="pspC"
                     /locus_tag="PLS34_11510"
     CDS             2527..2922
                     /gene="pspC"
                     /locus_tag="PLS34_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HRP88126.1"
                     /translation="MKRDPSPTRFYRDEEAGKFGGVCAGLAEYFGFDLTLTRVVTVLA
                     MLFFPTVFLVYLVLWFVLPAKPRTLYRDESDKDFWRGMRVSPRETLSEVRHRFRSADA
                     KLQRMERYVTSRNFNLDREFQDLERGERR"
     gene            2955..3173
                     /locus_tag="PLS34_11515"
     CDS             2955..3173
                     /locus_tag="PLS34_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014997581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88127.1"
                     /translation="MNPFEMVVIIVFLSIVASMYSSWLKHKRKGGDTPVDDRRLASLE
                     ERVRVLERIVTDSGYDLRRQFRDLERDA"
BASE COUNT          545 a         1015 c         1123 g          494 t
ORIGIN      
        1 atggcggcgc ggtcccgcgg acaaccgcca accgaagcgc gcccatggcg gcgcgccgcc
       61 gccgtcgagc ggaaaccggt gtctgacccc ggttttcagc ggttttccgg gcgtcccggc
      121 cggccggcgc cgccgatgcc gtgcttgcgc agctggccgc gcagctggtc atagctcagc
      181 ccgagcgatt cggcggccac gcggcgcttg ccgtcggccg cggccagcgc gcgcgtcagc
      241 agccgttgct cgaagcccgc gaccgcgccc cggaagtcca gcggcagcgc cggggcctgc
      301 ggtgtgggat tgccggtgcc ggctgcagcc tgcgcatcgc cgtcgagggc tgccggctcg
      361 tcgccgtggt cgtgcgcaaa ggggtcgaag tgaatctcgt ccaccaggcg ctccgggtcg
      421 gcggcgcggt agatggagcg ctccacggcg ttcttgagtt cgcgcacgtt gccgggccac
      481 ggccaggcga ggagcgcggc caccgcggat tcgctgaagc cagggaagaa cggccgcttc
      541 aactcggcgg tgaagccgac ggcgaaatgc tgcgccagct cgagaatgtc ctccggccgg
      601 gcgcgcagcg gcggtacatg aatcacgtcg aaggccagcc ggtcgagcag gtccgcgcga
      661 aagcgccccg cccgggcgag cgcgggcagg ttctcgttgg tggcgccgac gatgcgcaca
      721 tcggcccgca gcgtctcgct gccgccaacc cgttcgaatt caccgtactc gatgacccgc
      781 aggatcttct cctgcatgcg cgccggcagg gtgcctagct cgtcgaggaa caggctgccg
      841 ccgtcggcgc gctcgaagcg gcctacgtgg cgccgcgtcg cgccggtgaa agcgcccgcc
      901 tcatgcccga acagctcgga ttccagcagg ctctcggtga gcgcggcgca gttgagcttg
      961 accaggggct gctcccagcg tggcgacaga tagtgcagcc gcgccgcgac cagctccttg
     1021 ccggtgccgc gctcgccgat gatcagcgcc ggccggtcga ggggggccag ccgggaaacc
     1081 tgctcgagca tggccccgaa agcgggtgag gcgccgagca tcgggctgct ggtatccacg
     1141 tccattggcg tattctgcta ggtattgggc aaagtgacca tataaaaaag gcgaaaaagc
     1201 ccacgttatc gcgtgcgcct aaaaaaagac catcaaaaac aaagtgttac gggctgccca
     1261 tggttggcac ggttactgca aagagtagtc caggacaccg gaggaaccgc catgggcatt
     1321 ttttcccgct tttcagacat catcaattcg aacatcaacg ccctgctgga gaaggccgag
     1381 gaccccgaaa agatggtccg gctcatcatc caggagatgg aagacacgct ggtcgaagtg
     1441 cgctcggcgg cggcccgcgc cattgcggat cgcaagcagc tggaccgccg gctcgccgag
     1501 gcccgccgcg aaggcgacga ctggagccgc aaggccgagc tggcggtgcg caagggtcgt
     1561 gacgacctgg cccgcgccgc gctgtcggag aaggcgcgcg ccgagcagca cgtggcctcg
     1621 ctggaagagc agggcgcgca ggtgcaggag gcgctcgccc ggctgaacga cgacatggcg
     1681 cgcctgcagg aaaagctggc ggatgccaag acgcgccaga aggcgatggt catgcggcat
     1741 cgcaccgccg gccaccggct cgagatgcgt cggcagattc acgagagcaa gatcgacgac
     1801 gcgctggtga ggttcgagca gttcgagcgt aagctcgagg acgtcgaggg gcgtgtggag
     1861 tcgtacgatc tcggccagcg caaggatctc ggccgcgagt tcgccagcct ggagtccgag
     1921 gagtccgtgg aggccgagct ggcggcgctg aagcggcgcg tgggcggcga gtcccgcggc
     1981 gaggcctgag cgaggccggg gacaaggact gaaggaagca agtcatgagt gagtttttcg
     2041 gcgttgtcat gatcatcgtt gcggccgtcg tgctgccgct ggtggtgatc ctgcactacg
     2101 tgacgaagtg gaagtcctcg cggaccctgt cgggcgacga gcagcgcatg ctcgaggagc
     2161 tctggcagga cgcgcagaag atggaaagcc gcatcaacgc gctggaaacc atcctggacg
     2221 accaggtgcc cgactggagg aggaggacat gaatcgccgc aagcacgcgc ccgccacggc
     2281 cattttctac cgcgaccccg ccaacggcat gatcatgggg gtgtgcgcgg gactgtcgga
     2341 ccagttcggc ttccgcctgg tgccgttgcg catcatcgcg gtggcggcgc tggtgttgtt
     2401 caccgtgccg accctgctgg cctacctggt ggccggggtg ttgctgcccg gcaagccgct
     2461 gacctattac ggccatcgcg acgagcgcga gctgtggggt cgtcgccgga ccgaaaggat
     2521 gcaggcatga agcgcgatcc cagcccgacc cgcttctatc gcgacgagga ggccggcaag
     2581 ttcggcggcg tctgcgcagg gctggccgag tacttcggct tcgacctgac gctgacgcgg
     2641 gtcgtcaccg tactggcgat gctgttcttt ccgacggtgt tcctggtgta cctggtgctg
     2701 tggttcgtgc tgcccgccaa gccgcggacg ctgtatcgcg acgagtcgga caaggacttc
     2761 tggcgcggca tgcgcgtatc gccgcgcgag acgctgtccg aggtgcgcca ccgtttccgc
     2821 agcgccgatg ccaagctgca gcgcatggag cgctacgtga cctcgcgcaa cttcaacctg
     2881 gaccgggagt tccaggatct cgagcgcggc gaacgccgtt agaataagac acaagtgttg
     2941 gaggagggct cgccgtgaat cctttcgaga tggtcgtcat cattgttttc ctgagcatcg
     3001 tagcctccat gtattcgagc tggctcaagc acaagcgcaa gggcggtgac acgcccgtcg
     3061 acgacaggcg cctcgccagc ctcgaggagc gcgtccgcgt gctcgagcgc atcgttacgg
     3121 attccggcta cgacctgcgc cgccagttcc gcgacctcga gcgggacgcc tgaccgc
//
LOCUS       DAOMPG010000260         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12446_length_3162_cov_3.023817, whole genome shotgun sequence.
ACCESSION   DAOMPG010000260 DAOMPG010000000
VERSION     DAOMPG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12446_length_3162_cov_3.023817"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLS34_11520"
     CDS             <1..326
                     /locus_tag="PLS34_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88128.1"
                     /translation="PKAEQIAAAVLATAGDLGVDVATVIPVCAAPGRAYNVDDALWAA
                     MLFELPEANRVRLLRCQRARRSAQDWGLLREQLRNAGRFLKSGVRNWGQTPFSRTKRG
                     SDPSF"
     gene            401..667
                     /locus_tag="PLS34_11525"
     CDS             401..667
                     /locus_tag="PLS34_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRP88129.1"
                     /translation="MRKITATELARNLRAVLDRVRESGEAYIVERGGLPVVQVSPTPG
                     EQTAEQALADLYRTLPPEAADGWREDARRGGRSLDDELHDPWAS"
     gene            655..1074
                     /locus_tag="PLS34_11530"
     CDS             655..1074
                     /locus_tag="PLS34_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP88130.1"
                     /translation="MGFLIDTCIWIDVEQGRLGPADVSAFTGEAPVYLSPVTLAELRL
                     GIEMTADPGVRQKRMATYRRMTRKPLLPIDADTADVFGELAGVLAKASGRTHRTRVQD
                     LWLASQAIQHSLGLVTRNPADFDDIPGLELVNIALPG"
     gene            complement(1128..2960)
                     /locus_tag="PLS34_11535"
     CDS             complement(1128..2960)
                     /locus_tag="PLS34_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88131.1"
                     /translation="MHGLLAELKRRRVFRVAGLYGVVAWIIAEVSSVVFPVLLLPEWT
                     VTFIVVVLMLGFPVAMVLAWAFDIGPQGIERTPPVAAAAGSTRRSQAWLVYGGLLVLA
                     MAALGWLLWARLMPPQLASYESVAVMPFANLSGDPANDYFSDGMAEELLNLLAQVPGL
                     QVAARTSSFAYRDQNVDVREIGRALGVDAVLEGSVRRSGERVRITAQLIDTGTGYHLW
                     SQNYDRQMADIFSVQDEISSEIVMALTGKLGTRQAPEGLPVRAAPTDSVEAYTLYLQA
                     RHQWKRRGAEPVRRSIALLEEALELDPEFARAWAGLAAAWVVLPGYVGEAAAEYHTRA
                     TDAARRALAQDPNLAEAHAVLALIQSHQGDWSAADAAYFFAISLDPNDATARHWYSLL
                     LAQAGRLREALEQATAAYDLDPASPIINFNLAQHLLALGYNERAERQLRSAVELGFDS
                     AADPGDRALLAYRRGDTTRFGELAGMYIRQVREDPELMAAVMAAFTDRAAIPQLEARL
                     DAAEVPVKSRSYLWMLLGDDARAIRALLAAAEHGMQDLAMVWFPEGRGVRMHPDFATL
                     AEATGLMDYWKQFGMPDDCRLVDGRLACGFTTLAAVADPVGVAP"
BASE COUNT          495 a         1083 c         1106 g          478 t
ORIGIN      
        1 gcccgaaggc cgagcagatc gcggcggcgg tgctcgccac ggccggcgac ctcggcgtgg
       61 acgtggcgac ggtgatcccg gtgtgcgccg cgcccgggcg tgcctacaac gtggacgacg
      121 cgctctgggc cgccatgctg ttcgagttgc ccgaggccaa ccgcgtgcgg ctgttgcgct
      181 gccagcgggc gcggcgctcg gcgcaggact ggggcctgct gcgcgagcag ctcaggaacg
      241 ccggccgctt cctgaaatcc ggggtcagaa actggggtca gacccccttt tcgcgaacga
      301 aaagggggtc tgaccccagt ttctgatccc gttttgctac ggcttgctcg aaatcattgg
      361 aatacataaa atcagaccga ctctgattcg agcaaattca atgaggaaga tcactgccac
      421 ggaactcgca aggaatctcc gcgcggtgct ggaccgcgtc cgggaatcgg gcgaggccta
      481 tatcgtggag cggggcgggc tgccggttgt ccaggtctca cccacgccgg gcgaacagac
      541 cgccgagcag gcgctggcgg acttgtaccg gacgctccca cccgaggctg ccgatggctg
      601 gcgggaggac gctcgccggg gcggccggtc gctggacgac gagttgcacg acccgtgggc
      661 ttcctgatcg atacctgcat ctggatcgat gtcgagcagg gccggctggg cccggccgac
      721 gtatcggctt tcaccggcga ggccccggtt tacctgtctc cggtgaccct ggccgagctg
      781 cgcctgggca tcgagatgac cgcggacccc ggtgtgcgcc agaagcgcat ggccacctat
      841 cggcgcatga ctcgcaagcc cttgctgccg atcgacgccg ataccgcaga cgtgttcggc
      901 gagctcgccg gggtactggc caaggccagc ggtcgcaccc atcgcacgcg cgtccaggac
      961 ctgtggctgg ccagccaggc aatccagcac agcctcggcc tggtcacgcg caatcccgcc
     1021 gacttcgacg acatccccgg cctcgagctg gtgaatatcg ccttgccggg ctaggaattg
     1081 gggtcagacc cccgaacaaa aagggggtct gaccccagtt tctgacccta cggggcgacg
     1141 ccgaccgggt cggccaccgc ggcgagagtg gtgaagccgc aggcgagccg cccgtcgacc
     1201 aggcggcagt cgtccggcat gccgaactgc ttccagtagt ccatcagccc ggtcgcttcg
     1261 gccagggtgg cgaagtcggg atgcatgcgc acgccgcggc cttccggaaa ccacaccatc
     1321 gcaaggtcct gcatgccgtg ctcggcggcg gcgagcaacg cccggatcgc gcgcgcgtcg
     1381 tcgccgagca gcatccagag atagctgcgg gacttgacag gcacttcggc ggcgtcgagg
     1441 cgcgcctcga gttgcgggat ggccgcgcga tccgtgaagg ccgccatcac cgccgccatg
     1501 agctccgggt cttcgcggac ctgcctgatg tacatgccgg cgagctcccc gaagcgcgtc
     1561 gtgtcgccgc gacggtaggc gagcagggcc cggtcgccgg ggtccgccgc gctgtcgaag
     1621 ccgagctcga cggccgagcg cagctggcgc tcggcgcgtt cgttgtagcc gagcgcgagc
     1681 aggtgctgcg ccaggttgaa gttgatgatc ggcgaggccg ggtcgaggtc ataggcggcc
     1741 gtggcctgtt cgagcgcctc cctgagtcgt ccggcctggg ccagcagcag cgagtaccag
     1801 tggcgcgccg tggcgtcgtt cggatcgagc gagatggcga agaaataggc cgcgtccgcg
     1861 gcgctccagt cgccctggtg gctctggatc agcgccagca cggcgtgcgc ctcggccagg
     1921 ttggggtcct gcgcgagggc gcggcgcgcg gcgtcggtgg cccgcgtgtg gtactcggcg
     1981 gcggcttcgc cgacatagcc cggcagcacc acccaggcgg cggccagccc cgcccaggcc
     2041 cgcgcaaact ccgggtcgag ctcgagcgct tcctcgagca gcgcgatgct gcgacgcacc
     2101 ggctcggcgc cgcgccgctt ccactggtgg cgcgcctgca ggtagagcgt gtaggcctcg
     2161 acgctgtcgg tcggcgcggc tcgcaccggc agtccctccg gcgcctggcg cgtgccgagc
     2221 ttgccggtca gcgccatcac gatctcgctg gagatctcgt cctgcacgga gaagatgtcc
     2281 gccatctgcc ggtcatagtt ctgcgaccac aggtgatatc ccgtgccggt gtcgatcagc
     2341 tgcgccgtga tccgcacgcg ctcgccggag cgcctcacgc tgccctcgag cacggcgtcg
     2401 acgcccagcg cgcggccgat ctcgcggacg tccacgttct gatcgcggta ggcgaaggag
     2461 gaggtgcgcg cggcgacctg caggccgggc acctgggcca gcaggttgag caattcctcg
     2521 gccatgccgt cgctgaagta atcgttggcg ggatcgccgc tgagattggc gaacggcatc
     2581 accgcgaccg actcgtagct cgcgagctgg ggcggcatca gccgcgccca cagcagccag
     2641 cccagcgccg ccatcgccag caccagcagc ccgccgtaca ccagccacgc ctgggagcgc
     2701 cgggtgctgc ccgcagccgc ggccaccggc ggcgtgcgct cgatgccctg cgggccgatg
     2761 tcgaaggccc aggccagcac catggcgacc ggaaagccga gcatcagcac gactacgatg
     2821 aaggtcacgg tccattccgg cagcagcaac acggggaaca ccaccgatga aacctcggcg
     2881 atgatccagg cgacgacgcc gtacagtccc gcgacgcgga acactcgccg tcgcttcagc
     2941 tcggccagca ggccgtgcat aggcgctccg ggtgggtgga cgattccctt attcagtgcg
     3001 gagaatgata tctccattga tggtttccat ggtcacccgg gcggatcccg ccccggccgt
     3061 gaaccgcagc tcccggccgg gggcgtagcg gctggtgcgc accggttccg gtccgaaggc
     3121 gttgttgatc tgcccgttca aggtttcgat cgtgaaatca gc
//
LOCUS       DAOMPG010000261         3142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12537_length_3142_cov_3.185941, whole genome shotgun sequence.
ACCESSION   DAOMPG010000261 DAOMPG010000000
VERSION     DAOMPG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12537_length_3142_cov_3.185941"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="bioH"
                     /locus_tag="PLS34_11540"
     CDS             complement(<1..746)
                     /gene="bioH"
                     /locus_tag="PLS34_11540"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HRP88132.1"
                     /translation="MRLHAETTGRGPELVLLHGWGLHSGVWAPLLPALAERHRVTCID
                     LPGHGCSDAAVDGFDLADAAAAVAALAPAGAAWLGWSLGGQVALAAALAGTDIGRLVL
                     VATTPRFLVAPDWPCAMAPATLAGFATALAGDHHRTLRDFLTLQLRGDRQAARLLGEL
                     RAVLAARPDPQPAALAAGLDILATTDLRAALPGLRRPVLVVAGERDRLTPAEAGRRMA
                     AGLPDARFLGLPGAAHAPFLSHPAAFLDAIG"
     gene            complement(743..1939)
                     /gene="bioF"
                     /locus_tag="PLS34_11545"
     CDS             complement(743..1939)
                     /gene="bioF"
                     /locus_tag="PLS34_11545"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRP88133.1"
                     /translation="MAKPRTTESLRTELARLEAAHLYRRRRVTAWHDRLGPAELLVDG
                     RRCLAFCSNDYLGLARDPRVAQAMSAGAVAWGAGSGAAHLVTGHTTAHHALEEALAEF
                     TGFPRALLFSTGYMANLGLVCALAGRGDLIAEDRLNHASLIDASRMSGARVRRYAHAD
                     PEAAERRLATTSGRRFIVTDGVFSMDGDGAPLQALAEVALRTDAALLVDDAHGLGVLG
                     RDGRGSLDAAGLAPSAVDALVGTLGKALGTFGAFVAGSEAVVETLIQRARSYIYTTAP
                     PPAVAAATLAALRLAREEGWRRERLAMLVARFRSGAAGLGLRLASSATPIQPVIAGEA
                     RAALAAAERLFERGLWVTAIRAPTVPAGSARLRITFSAAHREEDVDRLLEALAEIPEL
                     GAGALA"
     gene            complement(1917..2900)
                     /gene="bioB"
                     /locus_tag="PLS34_11550"
     CDS             complement(1917..2900)
                     /gene="bioB"
                     /locus_tag="PLS34_11550"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRP88134.1"
                     /translation="MPDPRCDWRLDEVRELFELPLFELLYRAHGVHREHFPRHQVQIS
                     TLLSVKTGACPEDCAYCPQSVRHDTGLTADPLLELESVVARARAARDSGATRFCMGAA
                     WRNPKPKQLAAVAAMVREVRALGLETCATLGMLTAEQAQALRVAGLDYYNHNLDTSEA
                     FYPEIITTRSYADRLETLQHVRDAGLKVCCGGILGMGETREDRAALLHTLATLPAHPE
                     SVPINQLVRVPGTPLAGAEPLDPFEFVRTIAVARVLMPASHLRLSAGRTEMSAELQAM
                     AFFAGANSIFYGEKLLTTPNPDADGDMALLQRLGLMPEPGPADAHGEAAHN"
BASE COUNT          436 a         1118 c         1205 g          383 t
ORIGIN      
        1 ccgatggcgt cgagaaaggc ggccggatgg ctgaggaacg gcgcgtgggc agcgcccggc
       61 aggcccagga aacgggcatc gggcaggccg gccgccatgc gccgcccggc ctcggccggg
      121 gtgagccggt cccgctcacc ggccacgacc agcacgggcc ggcgcaggcc gggcaaggcg
      181 gcgcgcaggt cggtggtggc caggatgtcg agcccggcgg cgagcgcggc gggctgcggg
      241 tcgggccgcg ccgccagcac ggcccgcagt tccccgagca ggcgggcggc ctgccggtcg
      301 ccgcgcagct gcagcgtgag gaaatcgcgc agcgtccggt gatggtcgcc ggccagcgcc
      361 gtggcgaagc cggccagggt ggcgggcgcc atggcgcagg gccagtcggg cgcgacgaga
      421 aatcgcggcg tggtcgccac cagcaccagc cggccgatat cggtccccgc cagcgcggcg
      481 gccagcgcga cctggccgcc cagcgaccag ccgagccagg cggcgccggc cggcgccagc
      541 gccgccacgg ccgcggcggc gtccgcgagg tcgaagccat cgactgcggc gtcgctgcag
      601 ccgtgtccgg gaaggtcgat gcaggtgacg cgatgccgct cagccagcgc gggcaacagc
      661 ggtgcccaga cgcccgagtg gagaccccag ccgtgcagca gcacgagctc gggtccgcgg
      721 ccggtggtct cggcgtgcag cctcatgcga gcgcacccgc gccgagctcc ggaatctccg
      781 ccagcgcctc gagcagccgg tccacgtctt cctcacggtg cgcggccgag aacgtgattc
      841 gcaggcgggc cgaaccggcg ggcacggtcg gcgcgcggat ggcggtcacc cacaggccac
      901 gctcgaacag ccgctcggca gcggcgagtg ccgcgcgcgc ctcgccggcg atgaccggct
      961 ggatgggcgt ggccgacgaa gcaagccgca gaccgagacc ggccgccccg ctgcggaagc
     1021 gcgccacgag catggccagg cgctcgcgcc gccagccttc ctcgcgggcg aggcgcagcg
     1081 ccgccagcgt cgccgcagcc accgccggcg gtggcgcggt ggtgtagatg tagctgcggg
     1141 cgcgctggat cagcgtctcg accacggcct cgctgccggc gacgaaggcg ccgaaggtgc
     1201 ccagcgcctt gcccagcgtg ccgaccagcg cgtccacggc gctcggcgcc aggccggcgg
     1261 cgtcgaggct gccgcgcccg tcgcggccga gcacgccgag cccgtgcgcg tcgtccacca
     1321 gcagcgccgc gtccgtccgc agtgcaacct cggccagcgc ctgcagcggt gcgccgtcgc
     1381 cgtccatgct gaacacgccg tccgtgacga tgaagcggcg gcccgacgtg gtcgccaggc
     1441 ggcgctcggc ggcctcggga tcggcgtgcg catagcggcg tacccgggcg cccgacatgc
     1501 ggctggcgtc gatcagcgag gcatggttga ggcgatcctc cgcgatcagg tcgccgcgtc
     1561 cggccagcgc gcagaccagg ccgaggttcg ccatgtagcc ggtggagaac agcagggcgc
     1621 gcgggaagcc ggtgaactcg gccagcgcct cctcgagcgc atggtgtgcc gtggtatggc
     1681 cggtcaccag gtgggcggcg ccgctgccgg cgccccaggc gacggcccct gcggacatgg
     1741 cctgcgcgac gcgcgggtcg cgcgccaggc cgagataatc gttgctgcag aacgccaggc
     1801 agcgccgtcc gtcgaccagc agctcggcgg gaccgagtcg gtcatgccac gcagtgacgc
     1861 gccgccgccg atagaggtgc gccgcctcga gccgcgcgag ctcggtgcgc agcgactcag
     1921 ttgtgcgcgg cttcgccatg ggcgtccgcc ggccccggct ccggcatgag cccgagccgc
     1981 tgcagcagcg ccatgtcgcc gtcggcatcc gggttcgggg tcgtcagcag cttctcgccg
     2041 tagaagatcg agttggcgcc ggcgaaaaac gccatggcct gcagctcagc gctcatctcc
     2101 gtgcggcccg cggacagccg caggtgcgag gccggcatga ggacgcgcgc gaccgcgatg
     2161 gtgcggacga attcgaaggg atcgagcggc tccgcgcctg cgagcggcgt gccgggcacg
     2221 cgcaccagct ggttgatcgg cacgctctcg ggatgcgccg gcagcgtggc cagggtgtgc
     2281 agcaaagccg cccggtcctc gcgcgtctcg cccatgccga ggatgccgcc acagcagacc
     2341 ttgaggccgg cgtcgcgaac gtgctgcaag gtctcgagcc ggtccgcgta gctgcgggtg
     2401 gtgatgatct ccgggtagaa ggcctcggag gtgtccaggt tgtgattgta gtagtccagc
     2461 ccggccacac gcagggcctg ggcctgctcc gccgtgagca tgcccagggt ggcgcaggtt
     2521 tcgagcccca gcgcacgcac ttcgcgcacc atcgcggcga ccgccgcgag ctgcttcggc
     2581 ttggggttgc gccatgcagc acccatgcag aaacgggtcg cgcccgagtc ccgtgcggcg
     2641 cgcgcgcggg ccaccaccga ttcgagttcc agcagcgggt ccgccgtcag cccggtgtcg
     2701 tgtcgcacgc tctgcgggca gtaagcgcag tcctccgggc aggcgccggt cttcaccgac
     2761 agcagtgtgc tgatctgcac ctggtgacgg ggaaagtgct cacggtgcac gccgtgggcg
     2821 cggtacagga gttcgaacaa tggcagttcg aacagctcgc gcacctcgtc gaggcgccag
     2881 tcgcaacggg gatcgggcat ggttggtggc tcgctgtcga ggcccggttc cgggcggaat
     2941 tcggcacaaa tcgggcatta cggccggggc cggccgcgcc gcacgatggg gccggagtat
     3001 tcgggtcgcg ggaacgggct gtcaacctgt gaggcgatgc aaagttgacc accacctgtt
     3061 gctgcgcctg ccgcgctggc cttgcgaact ttgcggcgcc gcggccggcg gcgccggcat
     3121 ctgcgccgcg tgccgggtgg ac
//
LOCUS       DAOMPG010000262         3124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12623_length_3124_cov_3.948843, whole genome shotgun sequence.
ACCESSION   DAOMPG010000262 DAOMPG010000000
VERSION     DAOMPG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12623_length_3124_cov_3.948843"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..463
                     /gene="rpsU"
                     /locus_tag="PLS34_11555"
     CDS             248..463
                     /gene="rpsU"
                     /locus_tag="PLS34_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRP88135.1"
                     /translation="MPGVRVRENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHAKRMQREQSRRKRLF"
     gene            505..951
                     /locus_tag="PLS34_11560"
     CDS             505..951
                     /locus_tag="PLS34_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRP88136.1"
                     /translation="MSVKTRLKDDMKQAMRSGDKARLGTVRMALAAIQQREVDERIEL
                     DDAAVLGVIEKMIKQRRESVEQYRAGKRDDLADKEAAEIEVLSSYLPEPLDAAELAAM
                     VDAAIAATGAASMKDMGRVMAELRAQAQGRADMAVLSATVKAKLAG"
     gene            1080..2837
                     /gene="dnaG"
                     /locus_tag="PLS34_11565"
     CDS             1080..2837
                     /gene="dnaG"
                     /locus_tag="PLS34_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRP88137.1"
                     /translation="MGLIPASFIDELMSRTDIVEVINSRVPLKKAGREYKACCPFHGE
                     KTPSFTVSPTKQFYHCFGCGAHGTALGFLMDYEHLGFPEAVEELASLAGLEVPREQSP
                     GAERRPDLYSVLDAAAAFYREQLKRTPAAVEYLKRRGISGATAATFGIGYAPDEWDGL
                     LRRVGTDDGAVQRLATAGMLIERDQGGHYDRFRGRVMFPIRDARGRVIAFGGRVLGKE
                     EPKYLNSPETPLFHKGRELYGLYEVRQALRHIPRLLVVEGYMDVVGLHEAGVNWAVAT
                     LGTATTPEHLERVFRVTEEVVFCFDGDRAGRQAAWRALENALPTLREGRQIRFLFLPE
                     GEDPDSLVRAEGAEAFTARLKDALPLSEYLHDELAGQVDMGSLDGRARLAELAKPLLA
                     RVPEGVFRALLVGRIATAVGMDEAGYARFVDAAAPAPARPAQPAARRRPAAPGRGSLV
                     RRAISLVVHFPARAAEARLSPELLESDRPGMDLLAALLEDVAQSPNLTTGGLLERWRE
                     HAAWPHLQKLATAELVTTADVALAELQDCLDRLEEERVRLRHEELFEISRSSRLTPEQ
                     RAELRNLDARLAKRPATGG"
     gene            2912..>3124
                     /locus_tag="PLS34_11570"
     CDS             2912..>3124
                     /locus_tag="PLS34_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRP88138.1"
                     /translation="MTDRRNALPDEKQSQIKLLIARGKEQGYLTYAEVNDHLPDDIVD
                     PEQIEDIVNTINDMGITVYEKAPDAEE"
BASE COUNT          513 a         1092 c         1076 g          443 t
ORIGIN      
        1 cacctggctg tagagggcgt gcgccagcaa gccgcgctcg gcgtcgaaca cggcgacgcc
       61 ggtctcgtca caggaggtct cgatacccag aacccgcaat gtgctgctcc tcggggccgc
      121 tgccctcgta tccgccagcg tctcacctac tggctttcgc cccgcggggc cgctagaatg
      181 gtcggtcttt acccggtcgg gccagtggcc gggccaagcc aagaaacctt caggacggtg
      241 agctttaatg cctggtgtac gcgtacgcga aaacgaatat ttcgatgccg ccctgcggcg
      301 cttcaagcgg gcctgtgaaa aggccggcgt cctgaccgag cttcgtcgca gagagtttta
      361 cgagaagccg acccaggagc gcaagcgcaa gaaggccgcg gcggtgaagc gccacgccaa
      421 gcgcatgcag cgcgaacagt cccgccgcaa gcggttgttc tgagcttacc cggaaccggc
      481 cgcccgcccg ggcgccccgc ctcgatgtcc gtcaagaccc gcctcaagga tgacatgaag
      541 caggccatgc gctccggcga caaggcccgc ctcggtaccg tccgcatggc gctggccgcc
      601 atccagcagc gcgaggtcga cgagcgcatc gagctggacg acgccgcagt gctcggggtc
      661 atcgagaaga tgatcaagca gcggcgcgaa tcggtcgagc agtaccgcgc cggcaagcgc
      721 gacgacctcg ccgacaagga agccgccgag atcgaggtgc tttcctctta cctgccggag
      781 ccgctcgacg cagccgagct ggcggccatg gtcgacgccg cgatcgccgc gaccggcgcc
      841 gcctcgatga aggacatggg ccgcgtcatg gccgagctgc gcgcgcaggc ccagggccgc
      901 gccgacatgg ccgtgctcag cgccacggtc aaggcgaagc tcgccggctg agccgcgcag
      961 ttcgcggcgc gccgcgaccg acactggatt tacggggcat ttgagctatt tatatcgggt
     1021 gcccacccgc gtccgcccac cccgagacac ccagaccgca tgacggatgc ctgccgccga
     1081 tgggcctgat tccggccagt ttcattgacg aactgatgag ccgcacggac atcgtggagg
     1141 tcatcaattc acgcgtgccg ctgaaaaaag cgggccggga gtacaaggcc tgctgtccct
     1201 tccatggcga gaagacgccg tccttcaccg tcagcccgac caagcagttc taccattgct
     1261 tcggctgtgg cgcgcacggc accgcgctcg gcttcctgat ggactacgag cacctcggct
     1321 tccccgaggc ggtcgaggaa ctcgcttcgc tggccgggct cgaggtgccg cgcgagcagt
     1381 cgccgggcgc cgagcgccgc cccgacctgt acagcgtgct ggacgccgcc gccgccttct
     1441 accgggaaca gctcaagcgc acgccggccg ccgtcgagta cctcaagagg cgcggcatca
     1501 gcggcgcaac cgcggccacc ttcggcatcg gctacgcgcc cgacgagtgg gacggactgc
     1561 tcaggcgcgt cggcacggac gacggcgcgg tgcagcgtct cgccacggcc ggcatgctga
     1621 tcgagcgcga ccagggcggc cattacgacc gcttccgcgg ccgcgtgatg tttcccattc
     1681 gtgatgcgcg cggccgggtg atcgcgttcg gcgggcgggt gctgggcaag gaggagccca
     1741 agtacctgaa ctcgcccgag acgccgctgt tccacaaggg ccgcgagctg tacgggctct
     1801 acgaggtgcg ccaggcgctg cgccacatcc cccgcctgct ggtggtcgag ggctacatgg
     1861 acgtggtcgg cctgcacgag gccggcgtca actgggcggt ggcgacgctc ggcaccgcca
     1921 ccacgccgga gcacctcgag cgtgtgtttc gcgtcaccga ggaagtggtg ttctgcttcg
     1981 acggcgaccg cgccggccgg caggccgcct ggcgggcgct ggagaacgcc ctgccgacgc
     2041 tgcgcgaggg ccgccagatc cgcttcctgt tcctgcccga gggcgaggac cccgactcgc
     2101 tggtgcgcgc cgagggggcg gaggccttca ccgcgcggct caaggacgcg ctgcccttgt
     2161 cggagtacct gcacgacgaa ctggccgggc aggtcgacat gggcagcctg gacggacgcg
     2221 cgcggctggc ggagctggcc aagcccctgc tcgcccgggt gccggagggg gtgttccgcg
     2281 ccctgctggt gggccgcatc gcgacggccg tgggcatgga cgaggccggc tatgctcgct
     2341 tcgtggacgc cgccgccccg gcgccggccc gccctgcgca gcccgccgcg cgccgccgcc
     2401 cggcggcgcc cgggcgcggc agcctggtgc ggcgggcaat ctcgctggtg gtgcactttc
     2461 cggcccgcgc cgccgaggcg cggctgtcgc cggagctgct ggagagcgac cgcccgggca
     2521 tggacctgct ggccgcgctg cttgaagacg tggcccaaag ccccaatctc actaccggcg
     2581 ggctgctgga gcgctggcgg gaacacgccg cctggcccca cctgcagaag ctggcgacgg
     2641 ccgagctggt gaccaccgcg gatgtggcgc tggcggaact ccaggactgc ctggaccgtc
     2701 tggaggagga gcgggtgcgc ctccgccacg aggaattgtt cgaaatctcc cgcagcagcc
     2761 ggctgacccc ggaacagcgg gccgaactgc gcaacctgga cgcccgcctg gcgaagcgtc
     2821 ccgcgaccgg tggctgagcc gccgcatcga aatattgcgc cgcagtgcta tactgtttga
     2881 ttcgttttcc accggcctag agggcttctg agtgaccgac agacgcaacg ccctacctga
     2941 cgagaagcag tcccagatca agctgctgat cgcccgcggc aaggagcagg gctacctgac
     3001 ctatgccgaa gtcaacgatc acctgccgga cgacatcgtc gatccggagc agatcgagga
     3061 catcgtcaac accatcaacg acatgggcat cacggtctac gagaaggcgc cggatgccga
     3121 agag
//
LOCUS       DAOMPG010000263         3087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12823_length_3087_cov_4.008905, whole genome shotgun sequence.
ACCESSION   DAOMPG010000263 DAOMPG010000000
VERSION     DAOMPG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12823_length_3087_cov_4.008905"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>261
                     /locus_tag="PLS34_11575"
     CDS             104..>261
                     /locus_tag="PLS34_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HRP88139.1"
                     /translation="MHWTRILGIALIVAGAIMLYMGWTATESFTEQASEALTGRYTED
                     TRIYLIGGA"
     assembly_gap    262..301
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(402..821)
                     /locus_tag="PLS34_11580"
     CDS             complement(402..821)
                     /locus_tag="PLS34_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88140.1"
                     /translation="MTRRILILALTVLLAGLLPLAAQADRVKTVKPGPEAAQIVGSPG
                     FPTRDLFAVTFIEINGQNISPRDVMWLEPGTYRITVLIDAEQTRPPQIRRRSRDEPGY
                     NVIELELEAGKTYHIRGRYHRDQPDRPYSVILHRVDE"
     gene            complement(937..1845)
                     /locus_tag="PLS34_11585"
     CDS             complement(937..1845)
                     /locus_tag="PLS34_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRP88141.1"
                     /translation="MSQYRFDQFVFDASDGRLRHAASGAALTLRPQVARLLVALLEQP
                     NTVIDRDALCLAIWGEGAVVDFESGLAAIVRELRQALEQLGGAAGLVETVPRRGLRLG
                     ASVSATAAGEDHGSQSEPPTKGTLPRRAVLVALLVLAMALVWLLARWTMAPPAPTDTA
                     AEQEWTLAVLPFERFGEVPANEARLELLLADALLGRLWAAELEGVVLIGRATLAPYAS
                     REDVAGAVAMDLGVRLLIEGSIVQDAEGWRVTARLLHMPAGRVLWSDTIDWAGAAELP
                     VRDSAARLVESLAAEWAMRSQVADFQ"
     gene            complement(1951..2652)
                     /locus_tag="PLS34_11590"
     CDS             complement(1951..2652)
                     /locus_tag="PLS34_11590"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HRP88142.1"
                     /translation="MHGSGRRGNTPGRGEHEVHAMLTRFSRLTITALLAVGALALGGC
                     ASMNKDECLALDWRTIGYEDGVAGHSGDRVGQHRKACAKHGVAPDLDRYQQGRSEGLQ
                     EFCRPITGFRYGARGALYHGVCPEHLESGFVEAYEMGFHLYGLKARHRYAYSQLESKR
                     RELARSEQAIQDNALLIVSTSSTPEQRAQALLDTRQLAELVGRLKAEIEQLERDVVVY
                     ELDVEQYRASIPYSS"
     gene            2749..>3087
                     /gene="arfB"
                     /locus_tag="PLS34_11595"
     CDS             2749..>3087
                     /gene="arfB"
                     /locus_tag="PLS34_11595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRP88143.1"
                     /translation="MDAFDDRDRPPFQPPENELTEKFVLTGGPGGQHVNRTESGVQLR
                     FDVPASTFLDDAVKARLLKLAGSRADSAGVITIDARRFRSQHRNRQDARARLARLIEQ
                     AHRAPRRRIAT"
BASE COUNT          478 a         1087 c         1049 g          433 t
ORIGIN      
        1 gcagtgccgg gcgacgacga gttcatcccg ctgccggaag agccgggcgg ctgagcggcg
       61 ttgctgctag gctgaatccc gactgccaca aggagaagcc gccatgcact ggacccggat
      121 cctcggaatc gcgctgatcg tcgccggcgc gatcatgctg tacatgggct ggacggcgac
      181 ggagagtttc accgagcagg ccagcgaggc gctcaccggc cgctacaccg aggacacgcg
      241 catctacctg atcggcggcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 ngcgtcaagc gctgacggaa aaccggggcc ggaaacccgg ggtcagacac gggtttcgcg
      361 agcgaaaccc gtgtctgacc ccggttttcc ggcaacgcgg cctattcgtc gacccggtgc
      421 aggatcacgc tgtagggccg gtccggctgg tcgcggtgat agcggccgcg gatgtgatag
      481 gtcttgccgg cctcgagctc cagctcgatg acgttgtagc cgggctcatc gcgggaacga
      541 cggcgaatct gcggcggacg cgtctgctcc gcatcgatca gcaccgtgat ccggtaggtg
      601 ccgggctcga gccacatcac gtcacgcggc gagatgttct ggccgttgat ctcgatgaac
      661 gtgaccgcga acaggtccct ggtcgggaag cccggcgagc cgacgatctg tgcagcttcc
      721 ggccccggct tcacggtctt gacgcggtcg gcctgggccg ccagcggcag caggcccgca
      781 agcagcacgg tcaatgcaag tatgaggatg cgcctggtca tttcgtaact cccatctgtc
      841 agtctggaca cgaccacagc atcggcccgg cggccccgac gcaccatgtg aggaccctga
      901 aacattgctg atatttccct gggatgcgcc ggtgatttac tggaaatcag cgacttgcga
      961 tcgcatggcc cattcagccg cgagactttc gacgagccgc gcggcgctgt ccctgaccgg
     1021 cagctcggcg gcgccggccc agtcgatggt gtccgaccac agcacccggc cggccggcat
     1081 gtgcagcagc cgggccgtga cacgccagcc ctcggcgtcc tgcacgatgc tgccctcgat
     1141 gagcagccgc acgcccagat ccatcgccac cgcgccggcg acgtcctcgc gggaggcata
     1201 gggcgccagc gtcgctcggc cgatcagcac gacgccctcg agttcggccg cccagagccg
     1261 gcccagcagc gcgtcggcca gcagcaactc cagccgcgcc tcgttggcgg gcacctcgcc
     1321 gaagcgctcg aagggcagca cggcgagcgt ccattcctgt tcggccgccg tgtccgtcgg
     1381 cgccggcggc gccatcgtcc aacgcgccag cagccacacc agcgccatgg ccagcaccag
     1441 caaggccacc agcaccgcgc ggcgcggcag cgtgcctttc gtcggcggct cggactgcga
     1501 accgtgatcc tcgccggcgg ccgtcgcgga cacgctggcg cccaggcgca ggccgcgccg
     1561 cggcaccgtc tccaccaggc cggcggcccc gccgagctgc tccagcgcct ggcgcagctc
     1621 gcgcacgatc gccgccaggc cggactcgaa gtcgaccacc gcgccctcgc cccagatggc
     1681 gaggcacagc gcatcgcgat cgatcacggt gttcggctgc tccagcagcg cgaccagcag
     1741 ccgcgcgacc tgcgggcgca gcgtcagcgc ggcgccgctc gctgcatgcc ggagccggcc
     1801 gtcgctggcg tcgaaaacaa actggtcgaa gcggtactgg gacaccctgg cccggtctcc
     1861 tgcctgcggc acggcgcagg acctggaagc taaaactctg ctacaaggat accgcggcga
     1921 cctccggagg cgtaccggag cgaacggaac tcagctgctg taggggatgc tcgcccggta
     1981 ctgctcgacg tccagctcgt agacgaccac gtcgcgctcg agctgctcga tctcggcctt
     2041 gaggcggccg accagctcgg cgagctggcg ggtgtcgagc agcgcctggg cgcgctgctc
     2101 gggcgtggag ctggtgctga cgatcagcag tgcattgtcc tggatggcct gctcgctgcg
     2161 cgccagctcc cggcgcttgg actccagctg gctgtaggcg tagcgatggc gcgccttcaa
     2221 cccgtacagg tggaagccca tctcgtaggc ctcgacgaag ccgctctcga gatgctccgg
     2281 acacacgccg tgatacagcg cgccgcgcgc gccgtagcgg aagccggtga tcggccggca
     2341 gaattcctgc agtccctcgc tgcgtccctg ctggtagcga tccaggtccg gggcgacacc
     2401 gtgcttcgcg caggccttgc ggtgctgtcc cacccggtcg ccggagtggc cggcgacgcc
     2461 gtcctcgtag ccgatggtgc gccagtccag cgcgagacac tcgtccttgt tcatggaggc
     2521 gcagccgccc agcgcgagcg cgccgaccgc cagcagggcg gtgatcgtga gccgagagaa
     2581 gcgggtcagc atggcatgca cctcgtgttc gccgcggccg ggcgtgttgc cgcggcgtcc
     2641 tgacccgtgc acggtagcgg ggccgccccg gcgccgatgt gaggcgattc acagtcccgg
     2701 cggcgaccga ctggcgccgg cctgccgatt ggcgggatga tgcagagcat ggacgccttc
     2761 gacgaccggg accggccgcc cttccagccg ccggagaacg agctcaccga gaaattcgtg
     2821 ctgaccggcg ggccgggcgg ccagcacgtc aaccgcacgg agtcgggcgt gcagctgcgc
     2881 ttcgacgtgc cggcctcgac cttcctggac gacgcggtga aagcacggct gctgaaactc
     2941 gccggcagcc gcgccgattc cgccggggtg atcaccatcg atgcgcggcg ctttcgcagc
     3001 cagcaccgca accgccagga cgcgcgcgcg cggctcgccc ggctgatcga gcaggcgcac
     3061 cgggcgccgc gcaggcggat cgccacg
//
LOCUS       DAOMPG010000264         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_12976_length_3060_cov_3.268885, whole genome shotgun sequence.
ACCESSION   DAOMPG010000264 DAOMPG010000000
VERSION     DAOMPG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_3060_cov_3.268885"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..444)
                     /locus_tag="PLS34_11600"
     CDS             complement(16..444)
                     /locus_tag="PLS34_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP88144.1"
                     /translation="MFGKKKPVTSTIATLIGAGTTVTGDLVFRGGLHLDGRIVGDVTG
                     EPGAATKLTIGADGLIEGAVAVDDLVLGGAVHGDVTGREKVELGASSRVEGNVTYKVL
                     QMAAGARVNGRLIHQPGGQQALPPPAAEDEAGTATPGDED"
     gene            complement(459..1166)
                     /locus_tag="PLS34_11605"
     CDS             complement(459..1166)
                     /locus_tag="PLS34_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88145.1"
                     /translation="MDPQRLVVRLHDPRRFRFVVAAAVVLALLLCFGLYELGQRAGGH
                     SVFKSDQRRQTLRAEVRRLEGENQQLKDELARVRTSMEVDREAQARISESLAASETRV
                     AELSEELEFYRRIVSPQEGRAGLRVQSFEVLPGDLANAYRLKLLLVQSPQRSGRAQGQ
                     LELRLRGTLDGEEASLTTEQVAAEPRPFEFLYFQDIDIEVILPEGFVPESVDIDLRSG
                     QRNARPVTANFPWRPRG"
     gene            complement(1197..1727)
                     /locus_tag="PLS34_11610"
     CDS             complement(1197..1727)
                     /locus_tag="PLS34_11610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRP88146.1"
                     /translation="MKDCIDEDGFRANVGIILTNDTGRVFWAGRAGRPGWQFPQGGIL
                     PGESPRDAMFRELEEEIGLTREDVCVLGTTRGWLRYRLPRRYIRHHSQPVCIGQKQRW
                     FLLRLVSDEGRVRFDCCDHPEFDRWCWVNWWHPVSEVIYFKRRVYLRALREFGRLVFP
                     DGPPPRPGSGPGQPVQ"
     gene            1906..>2397
                     /locus_tag="PLS34_11615"
     CDS             1906..>2397
                     /locus_tag="PLS34_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRP88147.1"
                     /translation="MLSPALARPAWLSGRALATLAALAVLALAALGVALAAGSVALGP
                     GELWRGLVDADAGLASTLVRELRLPRALSAFAIGGLLALAGALMQILLRNPLADPYVL
                     GVSGGAATFALLVLAAGLGGMWVHGGAFLGALTATLLVFALAHGTGAWTPLRLLLTGV
                     VVAA"
     assembly_gap    2398..2430
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2431..2928
                     /locus_tag="PLS34_11620"
     CDS             <2431..2928
                     /locus_tag="PLS34_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRP88148.1"
                     /translation="ILALGPDSSLRGMLFWLMGDLSFARDPGPLLGLLALAMAAGVLT
                     GRHLNVLARGDARAETLGVAVRPLRIGIYVAGSLLTAIAVATAGSIGFVGLVVPHLVR
                     RLVGPDHRLLVPAAVLGGGTLLMLADTAARTVAAPRQLPVGALTALIGVPLFLVLMRR
                     QRSVT"
BASE COUNT          381 a         1110 c         1053 g          483 t
ORIGIN      
        1 ccccacgggc ccgggctagt cctcatcgcc cggcgtcgcg gtccccgcct catcctctgc
       61 cgccggcggc ggcagcgcct gctggccgcc gggctggtgg atcaggcggc cgttgacgcg
      121 cgcgccggcg gccatttgca gcaccttgta ggtgacgttg ccttccaccc gggagctggc
      181 gcccagttcc actttctcgc ggccggtgac gtcgccatgc acggccccgc ccagcaccag
      241 gtcgtccacg gccaccgcgc cctcgatgag accgtccgcg ccgatggtca gcttggtcgc
      301 cgcaccgggc tcccccgtga cgtcgccgac gatcctgccg tcgaggtgca ggccgccgcg
      361 gaacacgaga tcgccggtca ccgtggtgcc ggccccgatc agcgtggcga tggtggaagt
      421 cacgggtttc ttcttgccga acattgtcgt tatgcgcctc agcctcgcgg ccgccaggga
      481 aaattcgccg tcacgggtcg cgcgttgcgc tgcccggaac gcaggtcgat gtcgaccgac
      541 tcgggcacga agccctccgg caggatgacc tcgatgtcga tgtcctggaa gtacaggaac
      601 tcgaacggcc ggggctccgc ggcgacctgc tccgtcgtca gactcgcctc ctcgccgtcc
      661 agtgtaccgc gcaagcgcag ctccagctgt ccctgggccc ggccggagcg ctgcggggac
      721 tgcaccagca gcagtttcag ccggtaggcg ttggccaggt cgccgggcaa tacttcgaag
      781 gactgcacgc gcaggcccgc gcgcccttcc tgcggcgaga cgatgcggcg gtagaactcc
      841 agctcctcgc tgagctccgc cacgcgcgtc tcgctggcgg cgagcgactc gctgatgcgc
      901 gcctgggcct cgcggtccac ttccatgctg gtgcgcaccc gcgccagctc gtccttcagc
      961 tgctggttct cgccctccag ccgcctgacc tcggcccgca gtgtctggcg gcgctggtcg
     1021 ctcttgaaga cggaatgccc gccggcccgc tggcccagct cgtaaagccc gaaacacagc
     1081 agcagggcca gcaccacggc ggcagcgacc acgaaacgaa agcggcgcgg gtcatgcagc
     1141 ctgacgacga ggcgttgcgg gtccaagaat cctcccgtgg acggacggcg ccggcgctac
     1201 tgcaccggtt gcccggggcc tgaccctgga cgaggcggcg ggccgtccgg aaacacgagc
     1261 cggccgaatt cacgcagcgc gcgcaggtag acgcgccgct tgaagtagat cacttcgctg
     1321 accgggtgcc accagttgac ccagcaccag cgatcgaact ccggatgatc acagcaatcg
     1381 aatcgcacgc ggccctcgtc ggacaccagc cggagcagga accagcgctg cttctggccg
     1441 atgcagaccg gctggctgtg gtggcggatg taacgccggg gcagccggta tcgcaaccag
     1501 ccgcgcgtcg tgccaagcac gcagacatcc tcgcgcgtca gcccgatctc ctcctcgagc
     1561 tcgcggaaca tcgcatccct gggactctcg cccggcagta ttcccccttg cgggaactgc
     1621 cagcccgggc ggccggcccg tccggcccag aaaacgcggc ccgtatcgtt cgtgaggatg
     1681 atgccgacgt tggcccggaa gccgtcctcg tcaatgcaat ccttcatcgg gtgccgtttc
     1741 aattcgcttt gcagcagatt cttccacacg gggaccggga aaccaagcgt gccgcccggg
     1801 cctgccgcgc gacggggcgg accgggcatg ctagcctgtc gcgcctggca agccgcagcg
     1861 atccaactgg ccgacgctcc cttgatgtcc cgccccgccg ccccgatgct cagtcccgcg
     1921 ctcgcacggc ccgcctggct gtccggccgc gcgctggcga ccctggccgc tctggccgtg
     1981 ctggcgctgg ccgcgctcgg cgtcgcgctg gcggcgggca gcgtggcgct cgggccgggc
     2041 gagttgtggc gcggcctggt ggatgccgac gccgggctgg cgagcacgct ggtgcgcgag
     2101 ctgcgcctgc cgcgggcgct cagcgccttc gcgatcggcg ggctgctggc gctggcgggc
     2161 gcgctgatgc agatcctgct gcgcaacccg ctggccgatc cctacgtgct cggcgtgtcg
     2221 ggcggcgcgg cgaccttcgc cctgctggtg ctggccgcgg ggctgggcgg catgtgggtg
     2281 cacggcgggg ctttcctggg cgcgctgacc gcaactttgc tggtgttcgc gctggcccac
     2341 gggaccggcg cctggacgcc gctgcgcctg ctgctcaccg gcgtcgtggt ggctgccnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcctcgcgc tcggcccgga ctccagcctg
     2461 cgcggcatgc tgttctggct gatgggcgat ttgtctttcg cgcgcgatcc cggcccgttg
     2521 ctcggcctgc tggcgctggc catggccgcc ggcgtactga ccggcaggca cctgaacgtg
     2581 ctggcgcgcg gcgacgcccg cgccgagacc ctgggtgtcg cggtgcggcc gctgcgcatc
     2641 ggcatctacg tggccggctc cctgctgacc gccatcgccg tggccaccgc cggcagcatc
     2701 ggcttcgtcg ggctggtggt gccccacctg gtgcgccgcc tggtcgggcc ggatcaccgg
     2761 ctgctggtgc cggccgcggt gctgggcggc gggacgctgc tgatgctggc ggataccgcg
     2821 gcccgcaccg tcgccgcgcc gcgccagctg ccggtcggcg ccctcaccgc cctgatcggg
     2881 gtgccgctgt tcctggtgct gatgcgccgc cagcgcagcg tgacctgaat tggggacatt
     2941 ccccaataac ccggcgggtt attggggaat gtccccaatt gcgcgcggga acaccacgac
     3001 gtggtccgcc tcgaggcgga tgccgattcg ctcgccgatg cgatggtcgt gatggctcgg
//
LOCUS       DAOMPG010000265         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13143_length_3029_cov_3.213853, whole genome shotgun sequence.
ACCESSION   DAOMPG010000265 DAOMPG010000000
VERSION     DAOMPG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13143_length_3029_cov_3.213853"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="PLS34_11625"
     CDS             complement(<1..1910)
                     /locus_tag="PLS34_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP88149.1"
                     /translation="MTAALLQVDPGLRVFHRNGTLFAGRLSGTMRLQRIGPPDLVLLS
                     MFLLPGAPEARARGFVEGPGTRLFSTPPPLANVLARIAQLRDAGLLVAGAEQDGDVRV
                     ASTLQDCSGCAPVAPDSGWRLSSNFLLQPTPAGFAVNSPAVPHPIVLDIGLALLLTEF
                     AAGRAAGEVAAAHPALGSTEQRLKAVAWLAERRVLLPQEAGTEALAAPAQPAAFAVNR
                     STGDKPAWQLLEPDGRIPVYFVPHMENHYPLALGMIAAAMRVHAGGRLMERFQLLPIT
                     YMSPQELIDGPYRRFGPGVWLFSNYMWSLAVNLQVSAAVKHLDGRNLTLHGGPSTPNY
                     EAACEAFYARHGSVDMAVHGEGEIATAEAFECLRREADGRIGFDHEALAQVAGVSFRD
                     PAAAARVVRAPARMRLRQLDALPSPYLEGVFDDYAGRVEAAIVETNRGCPFACTFCDW
                     GSATNGKIAKFEHGRVRDEIEWIGRNQVRVLWIADANFGMLPADLEFAEWVVATKRRY
                     GFPQEVVVNYTKNANTRLADIIRVFSGAGIVSQGIISIQTTDEETLRIIDRQNIKTSK
                     YDELLRVFADAGLPLSTDLMIGLPGATVASFARDLQRYLDVDVAVKAYPTQLLPNSPM
                     AAPEYREKYRIEV"
     gene            2077..2352
                     /locus_tag="PLS34_11630"
     CDS             2077..2352
                     /locus_tag="PLS34_11630"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HRP88150.1"
                     /translation="MNNLLRASLVGALLAAAPTALLADDIEGRIEAVNPADQSFLLQG
                     ITFYTTPSTDYDDGLRSFADLAVGLKVEVDFSYIDGRHIATEIELDD"
     gene            complement(2378..>3029)
                     /locus_tag="PLS34_11635"
     CDS             complement(2378..>3029)
                     /locus_tag="PLS34_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP88151.1"
                     /translation="VFGSAIGGRIGTIDGVSYSAFIVPGLIMLTVFTESLSNAAFGIY
                     MPKFTGTSYELLSAPISPLETVLAFVGAAATKSVILGLVILATAGAFVPLELQHPGWM
                     FGYLVLVALAFSLAGFIIGLLAQGFEALQFVPMLIITPLSFLGGAFYSIRMLPETFQA
                     ISLFNPIVYVISGFRWSFYGTGDVAIGVSVAVLAAFLAASFAFVWWVFRSGYRLKS"
BASE COUNT          529 a         1036 c         1035 g          429 t
ORIGIN      
        1 acctcgatgc ggtatttctc gcggtactcc ggcgccgcca tcgggctgtt gggcagcagc
       61 tgggtgggat aagccttcac cgccacgtcg acgtccaggt aacgctgcag gtcgcgcgcg
      121 aagctggcca ccgtggcccc gggcaggccg atcatcaggt ccgtggacag cggcaggccg
      181 gcgtcggcga agacccgcag cagttcgtcg tacttcgagg tcttgatgtt ctggcggtcg
      241 atgatgcgca gcgtctcctc gtccgtcgtc tggatggaga tgatgccctg gctgacgatg
      301 ccggcgccgg agaagacgcg aatgatgtcg gccagccgcg tgttggcatt cttggtgtag
      361 ttgacgacca cttcctgggg aaagccgtag cggcgcttgg ttgccaccac ccactcggcg
      421 aactcgaggt cggccggcaa catgccgaag ttggcgtccg cgatccacag cacccgcacc
      481 tggttgcggc cgatccactc gatctcgtcg cgcacccggc cgtgctcgaa cttggcgatc
      541 ttgccgttgg tcgccgagcc ccagtcgcag aaggtgcagg cgaacggaca gccgcggttg
      601 gtctcgacga tggccgcctc gacgcgcccg gcgtaatcgt cgaacacgcc ttcgaggtaa
      661 ggcgagggca aggcatcgag ctggcgcagg cgcatgcggg ccggcgcgcg caccacccgg
      721 gccgcagcgg ccgggtcgcg aaacgagacc ccggccacct gcgccagcgc ctcgtgatcg
      781 aagccgatgc gcccgtcggc ctcgcgccgc aggcactcga aggcctcggc cgtggcgatc
      841 tcgccctcgc cgtgcaccgc catgtccacc gagccgtggc gggcgtagaa cgcctcgcag
      901 gcggcctcgt agttgggcgt gctcggccca ccgtgcaggg tcaggttgcg gccgtcgagg
      961 tgcttcaccg cggccgagac ctgcaggttc acggccagcg accacatgta gttggagaac
     1021 agccagacgc cgggaccgaa acgccggtac gggccgtcga tcagttcctg cgggctcatg
     1081 taggtgatgg gcagcaactg gaagcgctcc atcagccgcc cgcccgcgtg gacccgcatg
     1141 gcggcggcga tcatgccgag cgcaagcgga tagtggttct ccatgtgggg cacgaagtaa
     1201 acggggatgc ggccgtccgg ctccagcagc tgccacgccg gcttgtcgcc ggtcgaccgg
     1261 ttgacggcaa aggccgccgg ttgcgccggt gcggcaagcg cttcggtgcc ggcctcctgc
     1321 ggcagcagca cgcgccgttc cgccagccag gcgaccgctt tcagccgctg ctccgtcgag
     1381 ccgagcgccg gatgcgccgc tgcgacctcg cccgccgcgc ggccggccgc gaactccgtg
     1441 agcagcagcg ccagcccgat gtccagcacg atcgggtgcg gcaccgccgg ggaattcacg
     1501 gcaaaacccg ccggcgtcgg ttgcagcagg aaattgctcg acaggcgcca gcccgagtct
     1561 ggcgcgaccg gcgcgcaacc cgagcagtcc tgcagcgtgc tcgcgacgcg aacgtcgccg
     1621 tcctgctcgg caccggcaac cagcaggccc gcgtcacgca gctgcgcgat acgcgccagc
     1681 acattggcca gcggcggcgg cgtggagaag agccgggtgc ccggaccctc gacgaagccg
     1741 cgcgcccggg cctccggggc gcccggcagc aggaacatcg agagcaggac caggtcgggc
     1801 gggccgatgc gctgcaggcg catggtgccg ctcaggcggc cggcgaagag cgtgccgttg
     1861 cggtgaaaaa cccgcagccc gggatcgacc tgcagcagtg cagcagtcat gacgaggcat
     1921 cccgttgtag cgaccggcag tatccggggg ctgccggaca gggtcaaggt ggcgggccgc
     1981 cgcgtggttt gccgggccgc ggtagaatat cgttcagccg gcgttcagcc gccgatcgca
     2041 agatgctccg gaaaccccac ggacaggaga tccatcatga acaatctgct tcgcgcctcc
     2101 ctggtcggtg ctctgctcgc tgcggcgccg acggcattgc tcgccgacga catcgagggg
     2161 cgtatcgagg ccgtcaatcc ggccgaccag tccttcttgt tgcagggcat caccttctac
     2221 acgacaccct cgacggacta cgacgacggc ctgcgcagct tcgccgacct ggccgtcggc
     2281 ctgaaggtcg aggtcgactt cagttacatc gacggtcgcc acatcgccac cgaaatcgag
     2341 ctcgacgact gacagcaatg cgcggcggcg ccggagctca gctcttgagc cggtagccgc
     2401 tgcggaatac ccaccaaacg aaggcgaagc tggcggccag gaaggccgcc agcaccgcca
     2461 cgctgacgcc gatcgcgacg tcgccggtgc cgtagaagct ccagcggaag ccgctgatca
     2521 cgtacacgat cggattgaac aggctgatcg cctggaaggt ctccggcagc atgcggatcg
     2581 aatagaacgc cccgccgagg aagctcagcg gcgtgatgat caacatgggc acgaactgca
     2641 gcgcctcgaa accctgcgcc agcaacccga tgatgaagcc cgccaggctg aaggccagcg
     2701 ccaccagcac caggtaaccg aacatccagc ccgggtgctg caactccagc ggcacgaacg
     2761 ctccggccgt cgcgaggatc accagcccga gaatcaccga cttggtcgcc gcggcgccga
     2821 cgaaggcgag caccgtctcc agcggcgaga tcggcgcgga cagcagctcg tagctggtgc
     2881 cggtgaactt gggcatgtag atgccgaaag ccgcgttgct gaggctttcg gtgaacacgg
     2941 tcagcatgat cagcccgggc acgatgaagg cggagtagct caccccgtcg atggtgccga
     3001 tgcgcccacc gatggccgag ccgaacacc
//
LOCUS       DAOMPG010000266         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13230_length_3017_cov_4.776165, whole genome shotgun sequence.
ACCESSION   DAOMPG010000266 DAOMPG010000000
VERSION     DAOMPG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13230_length_3017_cov_4.776165"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLS34_11640"
     CDS             complement(<1..211)
                     /locus_tag="PLS34_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88152.1"
                     /translation="MTGILLGSSAAIAAGLAVVVLIFLLLSGEHPRLGAEIGMLLAST
                     GIFLGMTAVCAASFISLVKQLRWWWI"
     gene            350..2434
                     /locus_tag="PLS34_11645"
     CDS             350..2434
                     /locus_tag="PLS34_11645"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRP88153.1"
                     /translation="MTSAYLKVATLLLALLLAGTAAAKPTPASEVLAPSERHGKISRL
                     VTTLFERSHYRRAVVDDGTSSLMLDGYLDALDGNRQYFLAADIREFEAWRTRLDDAVK
                     RGQLDPVFAIYDRYLERARDRLEFALAQLELEHDFTADEDFMFDRSDEPWAASEAELD
                     DLWRKRVKNDALGLMLTDKNWDEAREILRGRYQQVLKRFGQVKSDDVFETFMNAFANT
                     MDPHTSYYSPRNSEEYRIQMSLNYDGIGATLQIQDEYVAVINVIEGGPAALDGTLKPK
                     DRIIGVGQGEDGEIVDVVGWRLDEVVDMIRGPGGTTVRLQILPGGAAPGSPPTVIPLV
                     RDKVKLEAQAARKDRIEVERDGRLHRIGVITVPSFYQDYNARASGAREYTSTTADVRR
                     LALELVDEGIDGLVLDLRNNGGGHLSEATAMTGLFLPGGPIVQLRDTSGRVEVLEEQA
                     TGPVYDGPLAVLVNRFSASASEIFAAAIQDYGRGVVIGQQTYGKGTVQNLYPLDRYAP
                     GADPRFGQLTLTIGKYYRVTGESTQHRGVVPDIVVPSPVDSHMVGESSRKTALPWDQI
                     RPTRFTPEGELDDAILMLTEEHGRRRAADPDFRFLMGDIEAIDERRSRKSVSLNIDAR
                     REENRRFDAERLARENERRVALGLEPLPSLEALEEQDGPDVLLREAAAVVADLAAVPE
                     AVLARHKGPAND"
     gene            2539..>3017
                     /locus_tag="PLS34_11650"
     CDS             2539..>3017
                     /locus_tag="PLS34_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRP88154.1"
                     /translation="MSQHSHLASFHAPAVCGTAVEMPARTLSVAAFAEGFRRASMSFL
                     PVLLTALLLVGCKGGDAPPEAAAQEKESAPIPVEVADLERGDVFAVYTGTASLETDAD
                     ALVVAKVAGEVVEILAEEGDRVKEGQVLARLDGDRLRLEMERARANLRKLEQEYNRSV
                     "
BASE COUNT          524 a         1027 c         1017 g          449 t
ORIGIN      
        1 gaatccacca ccagcggagc tgtttcacca ggctgatgaa actggcggcg caaaccgctg
       61 tcataccgag gaagatgccg gtgctggcga gcagcatgcc gatttccgcg cccagtcggg
      121 gatgttcccc ggacaggaga agaaagatca acaccaccac tgcaaggccg gccgcgatcg
      181 cggcggagga gccgagcagg atgccggtca cgacggtcat cggttgcatt gagtcaaatt
      241 ccgccttgtg tcgcgcttgc ctgccgcgcc cgaggccact agcctaagtg caggtcgcca
      301 gtctggccgg cggacccgcg ctgaccacgg agtcgagatg cgtattgtaa tgacatcagc
      361 ttatctcaag gtagccaccc tgttgctggc gctcctgctg gccggcactg ccgcggccaa
      421 gccgacgccg gccagcgagg tcctcgcgcc ctcggagcgg cacggcaaga tcagccggct
      481 ggtgaccacc ctgttcgagc gctcgcacta ccgccgcgcc gttgtggacg acggcacgtc
      541 ctcgctgatg ctggacggct acctcgatgc cctggacggc aaccgccagt attttctcgc
      601 cgccgacatc cgggagttcg aagcctggcg gaccaggctg gacgacgccg tcaagcgcgg
      661 ccagctggat cccgttttcg ccatctacga ccgctacctg gagcgggcac gcgaccgctt
      721 ggaattcgcg ctggcccagc tcgaactcga gcacgatttc acggccgacg aggatttcat
      781 gttcgaccgc agcgacgagc cgtgggctgc gtccgaagcc gagctggacg atctctggcg
      841 caagcgcgtc aagaacgacg ccctcggcct gatgctgacc gacaagaact gggacgaggc
      901 cagggaaatc ctgcgcggac gctaccagca ggtgctcaag cgcttcggcc aggtcaagag
      961 cgacgacgtg ttcgagacct tcatgaacgc cttcgccaac accatggatc cgcataccag
     1021 ctactactcg ccgcgcaact cggaggagta ccggatccag atgagcctga actacgacgg
     1081 catcggcgcc accctgcaga tccaggatga atacgtggcc gtcatcaatg tcatcgaggg
     1141 cgggccggcg gcactcgacg gcaccctcaa gccgaaggac cggatcatcg gggtcggcca
     1201 gggcgaggac ggcgagatcg tcgacgtcgt cggctggcgg ctcgacgagg tcgtggacat
     1261 gatccgcggg ccggggggca ccaccgtgcg gctgcagatc ctgccgggcg gcgccgcgcc
     1321 gggcagtccg ccgaccgtga tcccgctggt gcgcgacaag gtcaagctgg aggcgcaggc
     1381 ggcccgcaag gaccgcatcg aggtggagcg cgacggccgg ctgcaccgca tcggcgtgat
     1441 caccgtgccc tcgttctacc aggactacaa tgcgcgcgct tccggcgccc gcgagtacac
     1501 cagcaccacc gcggacgtcc ggcgcctggc gctcgagctg gtcgacgaag gcatcgacgg
     1561 gctggtgctc gacctgcgta ataacggcgg cggccacctg tccgaggcca ccgcgatgac
     1621 cggcctgttc ctgccgggcg gcccgatcgt gcagctgcgc gacaccagcg gtcgcgtgga
     1681 ggtgctcgag gaacaggcca cgggaccggt ttacgacggc ccgctggcgg tgctcgtcaa
     1741 tcgcttcagc gcgtccgcct ccgagatctt cgccgccgcc atccaggact acggccgcgg
     1801 cgtggtgatc ggccagcaga cttacggcaa gggcacggtg cagaacctct acccgctgga
     1861 ccggtacgct ccgggcgccg acccgcgctt cggccagctg acgctgacca tcggcaagta
     1921 ctaccgggtg accggcgaga gcacccagca ccgcggcgtg gtacccgaca tcgtcgtgcc
     1981 ctccccggtc gattcacaca tggtgggcga gagctcccgc aagacggccc tgccctggga
     2041 ccagatcagg ccgacgcgct tcacccccga aggtgaactc gacgacgcca tcctgatgct
     2101 gaccgaggaa catgggcgcc gccgggcggc cgatcctgac ttccgcttcc tgatggggga
     2161 catcgaggcc atcgacgagc ggcgcagccg caagtcggtc tcgctgaaca tcgacgcgcg
     2221 acgcgaggag aaccggcgct tcgacgccga gcgcctggcg cgtgagaacg agcgtcgcgt
     2281 tgcgctgggc ctcgaacccc tcccttcgct ggaggccctg gaggaacagg acgggcccga
     2341 tgtgctgttg cgcgaggccg ccgccgtggt ggccgacctc gcggccgtgc cggaagccgt
     2401 gctggcccgc cacaaggggc ccgccaacga ctgaccccgg ggcgggggca ctccgggggc
     2461 ttgcgtctgg ctaaaccggt gttatacttc actacaacac tactgtagtg aaccatcggt
     2521 caagtcgaga gcgtcgcaat gagtcagcat tcccatctcg cttccttcca cgccccggcc
     2581 gtctgcggca ccgccgtcga aatgccagcg aggaccctgt ccgtggccgc ttttgccgag
     2641 gggttccggc gggcttccat gtccttcctg ccggtcctgt tgaccgcctt gctgctggta
     2701 ggctgcaagg gcggcgacgc gccgccggag gccgccgcgc aggagaagga atccgccccc
     2761 atcccggtgg aggtcgccga ccttgagcgt ggcgacgtgt tcgccgtcta caccggcacg
     2821 gcctcgctgg aaacggatgc ggacgcgctg gtggtcgcca aggtggccgg cgaagtcgtc
     2881 gagatcctcg ccgaggaagg cgaccgggtg aaggaaggcc aggtgctggc ccgcctggac
     2941 ggtgaccggc tgcgcctcga gatggagcgg gcgcgcgcca acctgcgcaa gctggagcag
     3001 gaatacaacc gcagcgt
//
LOCUS       DAOMPG010000267         3007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13283_length_3007_cov_3.953930, whole genome shotgun sequence.
ACCESSION   DAOMPG010000267 DAOMPG010000000
VERSION     DAOMPG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13283_length_3007_cov_3.953930"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLS34_11655"
     CDS             <1..468
                     /locus_tag="PLS34_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRP88155.1"
                     /translation="GEFYSVALTNNYQQADTGTKMIHLGRNTRSTIVSKGISAGHGQN
                     AYRGLVKILPTAEGARNHTQCDSLLMGSDCGAHTFPYMEIRNPSAVVEHEATTSKISE
                     DQLFYCRQRGISEEDAVNMIVNGFCKEVFRELPMEFAVEAQNLLSVSLEGAVG"
     gene            494..1249
                     /gene="sufC"
                     /locus_tag="PLS34_11660"
     CDS             494..1249
                     /gene="sufC"
                     /locus_tag="PLS34_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRP88156.1"
                     /translation="MLSIKNLSARAGDRDILKGLDLEVGPGEIHAIMGPNGSGKSTLA
                     GVLAGRPDLEVTGGSVIYRGHDLLEMEPEERARAGVFLAFQYPVEIPGVNNVYLLKAA
                     LNAIRKHRGEPELDAFEFLTLVKEKMKLMQMDDSFLNRGVNEGFSGGEKKRNEVLQMA
                     VLEPVLAVLDETDSGLDIDALKVVANGVNSLRSPERSMVVVTHYQRLLDYIVPDFVHV
                     LSGGRIVRTGGRELALELEERGYDWLRDGADAA"
     gene            1246..2556
                     /gene="sufD"
                     /locus_tag="PLS34_11665"
     CDS             1246..2556
                     /gene="sufD"
                     /locus_tag="PLS34_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRP88157.1"
                     /translation="MSTATARQAAPPPARPDWIEAAAVAFDDPDWLAERRVEGLDAYA
                     AAGFPGRRDEDWKYTDLKLIARRRFAAGVTAAIETPALEGLDGPLLVFVNGRLIQARG
                     LPTGATVVPLAQAIVEGHPACRELLGRIARPERHRFAALATALFADGVLLDLADDLEL
                     HEVVRLVFLAGGEQPALACPRVLVRAGANSRATIVEHYPPAAGESLSLAVTEVALGPG
                     AMLQHYRLQEEASESFHLGVLAAKLDRDAALVSHNLSVGGRIARLDLDVELSAPGARV
                     EMNGLYVVGNGQHADSHTRVDHAAPHTTSDELYRGVLSGRSRAVFNGKAVVHPGAFGT
                     DAVQANANLLLSPQAEVDTKPELEIYADDVKCAHGATTGQLDTAALFYLRSRGIDEDT
                     ARTLLTFAFADTVLARMGLAALRRHAEQLVVGRLPEAERIREFT"
     gene            2553..>3007
                     /locus_tag="PLS34_11670"
     CDS             2553..>3007
                     /locus_tag="PLS34_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP88158.1"
                     /translation="MSEATLRTVAPGSGPGFDVEAVRQDFPILHQLVHGQPLAYLDSA
                     ASAQRPVQVIEAIAEYYRRDHANVHRGVYQLSQRATDAFEGARDKVRRFINAASVKEV
                     IFTRGTTEAINLVAQSWGRANLGQGDEILVTRMEHHANIVPWQMLCEQTG"
BASE COUNT          521 a         1038 c         1027 g          421 t
ORIGIN      
        1 ggcgagttct attcggtggc gctgaccaac aactaccagc aggccgacac cggcaccaag
       61 atgatccacc tcggccgcaa cacgaggagc accatcgttt ccaagggcat ctcggccggc
      121 cacgggcaaa atgcctatcg cggcctggtg aagatactgc ccaccgccga gggagcgcgc
      181 aaccacacgc agtgcgactc gctgctgatg ggcagcgact gcggcgcgca cacctttccg
      241 tacatggaaa tccgcaaccc cagcgcggtg gtcgagcacg aggccaccac ctcgaagatc
      301 agtgaggacc agctgtttta ctgccgccag cgcggcatct cggaagagga cgcggtgaac
      361 atgatcgtca acggcttctg caaggaagtg ttccgcgagc tgccgatgga gttcgcggtg
      421 gaggcacaga acctgctgag cgtgagcctc gaaggcgcgg tcggctgagc caggaatcac
      481 aggaaacgga ccaatgctaa gcatcaagaa cctgagtgcg cgcgccggcg accgcgacat
      541 cctcaaggga ctcgacctgg aggtcggccc cggcgaaatc cacgccatca tggggccgaa
      601 cggctccggc aagagcacgc tcgccggcgt gctggccggg cggccggatc tcgaggtcac
      661 cggcggcagc gtcatctacc gcggccatga cctgttggaa atggaacccg aggagcgcgc
      721 tcgcgccggg gtgttcctgg ctttccagta cccggtcgag atcccgggcg tgaacaacgt
      781 gtacctgctc aaggcggcgc tcaacgccat ccgcaagcac cggggcgaac cggaactgga
      841 cgccttcgaa ttcctcacgc tggtgaagga aaagatgaag ctcatgcaga tggatgacag
      901 cttcctgaac cgcggcgtca acgagggctt ttcgggcggc gagaagaagc gcaacgaggt
      961 actgcagatg gcggtgctgg agcccgtgct ggccgtgctc gacgagaccg actccggtct
     1021 cgacatcgat gcgctcaagg tggtggccaa cggcgtcaac agcctgcgct cgccggagcg
     1081 ctccatggtc gtggtgacgc attaccagcg cctgctcgac tacatcgtgc cggatttcgt
     1141 ccacgtactc tccggcggac gcatcgtgcg caccggcggt cgggagctgg ccctcgagct
     1201 ggaggagcgc ggctacgact ggctgcgcga cggagccgac gcggcatgag cacggccacc
     1261 gcccggcagg cggcgccgcc gcccgcgcgg cccgactgga tcgaagcggc cgcggttgcc
     1321 ttcgacgacc cggactggct ggccgaacga cgcgtggagg gcctcgacgc ctacgcggcg
     1381 gcgggctttc cgggccggcg cgacgaggac tggaaataca ccgacctgaa gctgatcgca
     1441 cggcgccgct tcgccgcggg cgtcaccgcc gcaatcgaga ccccggcgct cgaggggctc
     1501 gacggcccgc tgctggtgtt cgtcaacggc cggctcatac aagcccgggg cctgcccacg
     1561 ggcgccaccg tggtgccgct ggcacaggcg atcgtcgaag gccatccggc ctgccgggag
     1621 ctgctcggcc ggatcgcccg gccggagcgc caccgcttcg ccgccctggc gaccgcgctg
     1681 tttgccgacg gcgtgctgct cgacctggcc gacgacctgg aactgcacga ggtcgttcgg
     1741 ctggtattcc tggcgggcgg cgagcagccg gcgctggcct gcccgcgcgt gctggtgcgt
     1801 gccggggcga acagccgggc gaccatcgtc gaacactacc cccccgcggc cggggagtcg
     1861 ctcagcctgg cggtcaccga ggtcgcactc ggccccggcg caatgctgca gcactatcgc
     1921 ctgcaggaag aggccagcga gtcattccac ctcggcgtgc tggccgcgaa gctggaccgg
     1981 gatgcggcgc tggtgagcca caatctctcg gtggggggcc ggatcgcgcg cctcgacctg
     2041 gatgtcgagt tgtccgcccc gggcgcgcgg gtcgagatga acggcctgta cgtggtcggc
     2101 aacggccagc atgccgacag ccatacgcgc gtcgaccacg ccgcgccgca caccaccagc
     2161 gacgagctct atcgcggcgt gctctccggc aggagccgcg ccgtgttcaa cggcaaggcg
     2221 gtcgtccatc cgggcgcctt cggcaccgat gcggtgcagg ccaacgccaa cctcctgctg
     2281 tcgccccagg ccgaggtcga caccaagccg gaactcgaaa tctacgccga cgacgtgaag
     2341 tgtgcccacg gcgccaccac cggccagctg gacacggcgg cgctgttcta cctgcgctcg
     2401 cggggcatcg acgaagacac ggcgcggacg ctgctcacct tcgccttcgc ggataccgtg
     2461 ctggcgcgca tgggactggc ggcgctacgc cgccatgccg agcagctggt cgtcggcagg
     2521 ctgcccgagg ccgagcggat cagggagttc acatgagcga ggcgaccctg cgcacggtgg
     2581 cgcccgggtc cgggccgggc ttcgatgtcg aggcagtccg acaggatttc cccatcctgc
     2641 accagctggt gcacggccag ccgctggcct acctggacag cgcggcctcc gcacaacgtc
     2701 ccgtgcaggt catcgaggcg atcgccgagt actatcgccg cgaccatgcc aacgtgcacc
     2761 gcggcgtcta ccagctcagc cagcgcgcca ccgacgcctt cgagggggcg cgcgacaagg
     2821 tgcgtcgctt catcaacgcg gcgtccgtca aggaagtgat cttcacgcgt ggtaccaccg
     2881 aggcgatcaa cctggtggcg cagagctggg gccgcgccaa cctcggccag ggcgacgaga
     2941 tcctcgtcac gcgcatggaa caccacgcca acatcgtgcc gtggcagatg ctctgcgagc
     3001 agaccgg
//
LOCUS       DAOMPG010000268         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13381_length_2989_cov_4.476142, whole genome shotgun sequence.
ACCESSION   DAOMPG010000268 DAOMPG010000000
VERSION     DAOMPG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13381_length_2989_cov_4.476142"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2358)
                     /locus_tag="PLS34_11675"
     CDS             complement(<1..2358)
                     /locus_tag="PLS34_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRP88159.1"
                     /translation="MSGRALRGWLLGVVALGLVACGRGGEGEGQQPPALRTVTLSGVV
                     QKGRFQDLSVRAYSLSSATGERGEPVAATINLAQQSYSATLEASDLVLLEAVGTFTSE
                     ASGAPVALDQPLQTIVSVAALAPSDPVNINIATTLQAARYLEQRDSWAGTGDALLTDA
                     AALVNQALGFPAGTDAASLDLTAITATSTPQDPDLQLLLLSAALTASLDSPLLFASGF
                     DVIVDSFATAQDPAVAAAGLATLDGHTAQALYDLTVLNSGYPNLPNLVFPDPLILACI
                     VDRSCSWQVASGPVVTVSSPVVREADGEALLFLRLSEASTEPLALRVFTSGDSAASGR
                     DFVPTDSRVVIPAGRLEAQLRIPLIIDALPETDERFFVTVEIQTEDSGYAVAQPGEVL
                     IRDVLPRAREPSADALRVVELCALGTGDAGIVSVGPCNGAGGEGLGFVSDGDTVLAIG
                     MDLAADCPATGACAPQTADWRVGLFMVADDASGSPVAERALGDYLYPADAVQRLAEAP
                     DGRGVLASLAQPGLAQFGLDALGNGWSLRLEARIGARSPLVASAPVSSLLPLPATVQA
                     GGRSVPLASVTGLDPSGGTVCEGFDGAALEAGFLLGEIQGNPSIASGQVCVVLDYSDP
                     SSPTAELVAGAVDLAGVAISLPAGHGLVIGGGFPLPLMLSGFVVADGGEPSHLYHEGW
                     PFTLRVVGAELNPGGIEIRHAGIRYLMDVDYSAQDPRAQQNRGPDSNDIAYRAGAAGG
                     ALWLTDGGLQGVLEVAGGNGRTAFPLGGLSWQAFTQELQDSRLAGT"
     gene            2572..>2989
                     /locus_tag="PLS34_11680"
     CDS             2572..>2989
                     /locus_tag="PLS34_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HRP88160.1"
                     /translation="MSQDKHQDKDDSASLGPEPNPDAVVADTYDRLRRIAHQHLRRYR
                     PGATLNTTAVVHEAYIKLAGEAGGFWSDRNEFFKVASTVMRHLIVDYARRQQAEKRGG
                     GALMVTLNDRVPAAEGALLDVVALDDALRELGVLDSA"
BASE COUNT          490 a         1070 c         1033 g          396 t
ORIGIN      
        1 cgtgccggcc aggcggctgt cctgcagctc ctgggtgaag gcctgccagc tcagcccgcc
       61 cagcgggaag gcggtccggc cgttgccacc tgcgacctcc agcaccccct gcagcccgcc
      121 gtcggtcagc cacagcgcgc cccccgcggc gcccgcccgg taggcgatgt cgttgctgtc
      181 cgggccgcgg ttctgctgcg cgcgcggatc ctgcgccgag taatccacgt ccatcaggta
      241 acggatgccg gcatgccgga tctcgatgcc gccgggattc agctcggcgc cgaccacccg
      301 cagcgtgaac ggccagccct cgtggtacag gtggctcggc tctccgccgt ccgccaccac
      361 gaagccggac agcatcagcg gcagcgggaa gccgccgccg atgaccaggc cgtgtcctgc
      421 aggcaacgaa atggctacgc ccgccaggtc caccgcaccg gcgaccagct cggctgtggg
      481 actcgaggga tcactgtaat ccagcacgac acacacctgg ccgctggcga tactcgggtt
      541 tccctggatc tcgccgagca ggaagccggc ctccaacgcg gcgccgtcaa agccctcgca
      601 aaccgtgccg ccggagggat cgagcccggt cacgctggcc agcggcacgc tgcggccgcc
      661 cgcctggacc gtcgccggca acggcagcag ggaagaaacc ggtgcggatg cgaccagcgg
      721 cgagcgcgcg ccaatgcgcg cctcgagccg caggctccag ccattgccga gggcgtcgag
      781 cccgaattgc gccaggcccg gctgcgccag tgaggccagc acgccgcggc cgtccggcgc
      841 ttccgccagg cgttgtacgg catctgcagg atagaggtaa tcgcccagcg cgcgctccgc
      901 gaccggactg ccggaggcgt cgtcggccac catgaacaga ccgacccgcc agtcggccgt
      961 ctgcggcgcg caggcaccgg tcgccgggca gtccgccgcc aggtccatgc cgatggccag
     1021 caccgtgtct ccgtcactga caaagcccag gccctcgccc ccggcgccgt tgcagggccc
     1081 cacgctgaca atgccggcgt caccggtgcc gagcgcgcag agttcgacca cgcgcagcgc
     1141 atcggcggac ggctctcgcg cccgcggcag cacatcgcgg atcaacactt cgcccggctg
     1201 tgccaccgcg tagccggagt cctcggtctg gatttcgacc gtgacaaaaa agcgctcgtc
     1261 agtctccggc agcgcatcga tgatcagcgg gatgcgcaac tgtgcttcaa ggcgccctgc
     1321 gggaatcacc acgcggctgt ccgtggggac gaaatcacgc ccggacgcgg ccgaatcccc
     1381 gctggtgaac acgcggagcg ccagcggctc ggtggacgct tcgctgaggc gcaggaacag
     1441 caaggcctcg ccgtccgcct cgcgcaccac cggcgaggac accgtgacga ctggtccgga
     1501 tgcgacctgc cagctgcaac tgcggtccac gatgcatgcc aggatcaacg gatcggggaa
     1561 caccaggttg ggcaggttcg ggtagccgct gttgagtacg gtcaggtcgt acaaggcctg
     1621 cgcggtatgg ccgtcgagcg tcgccagccc ggcggccgcg accgccgggt cctgggcagt
     1681 cgcgaagctg tccacgatca cgtcgaagcc gctggcgaac agcagcggtg agtcgaggct
     1741 cgcggtgagg gcggcggaca aaagcagcag ttgcaggtcg gggtcctgtg gcgtggacgt
     1801 agcggtgatg gcggtgaggt cgagcgacgc ggcatcagtg ccggccggaa agccgagcgc
     1861 ctggttcacc agcgcggccg cgtcggtgag caacgcgtcg ccggtaccgg cccagctgtc
     1921 gcgctgctcc aggtagcgcg ccgcctgcag ggtggtggcg atgttgatgt tgaccgggtc
     1981 cgacggcgcc agggcagcca cgctgacaat ggtctgcaac ggctggtcga gggcgacggg
     2041 cgcgccgctg gcctcgctgg tgaaagtgcc gacggcctcg agcagcacca ggtcgctggc
     2101 ctcgagggtc gccgaatagg actgctgcgc gaggttgatc gtggcggcca ccggctcgcc
     2161 gcgctccccg gtggcgctgc tgagcgaata ggcccgcacc gacaggtcct ggaaacggcc
     2221 tttctgcacg acgcccgaaa gcgtcacggt ccgcagcgcc ggcggttgct gcccctcgcc
     2281 ttcaccacca cggccacagg ccaccaggcc gagcgcgacg acgcccagca gccagccacg
     2341 cagggcacgt ccggacacgc tgaaacggcg cgcagcaacc caccggcata ggtcggtcag
     2401 attcgacacg gtgctccctt ggctcgattc ggggccgctc ttccagcggc gcttctcatc
     2461 agggttaacg caggtgcgcg gaaaaacccg ccagagatgt gaaccatgtg tttgcccggg
     2521 ccgcccgggt cgatagactc gggtgccgtg ccaaccgacg accggacacc gatgagccag
     2581 gacaagcacc aggacaagga cgactccgcc agcctgggcc cggagccgaa cccggacgcc
     2641 gtggtggccg atacctacga tcggttgcga cgcattgcgc accagcacct gcgccgctat
     2701 cgcccgggtg caacgctcaa caccaccgcg gtggtccacg aggcctacat caagctggcc
     2761 ggcgaggccg gcggcttctg gagcgaccgc aacgagttct tcaaggtggc ctcgaccgtg
     2821 atgcgccacc tgatcgtcga ttacgcgcgg cgccagcagg ccgagaagcg cggcggcggc
     2881 gccttgatgg tcacgctcaa cgaccgcgtg cccgccgccg agggcgcgct gctggacgtg
     2941 gtcgcgctgg acgacgccct gcgcgagctc ggcgtgctcg attcggcgc
//
LOCUS       DAOMPG010000269         2963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13526_length_2963_cov_3.962517, whole genome shotgun sequence.
ACCESSION   DAOMPG010000269 DAOMPG010000000
VERSION     DAOMPG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13526_length_2963_cov_3.962517"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLS34_11685"
     CDS             complement(<1..690)
                     /locus_tag="PLS34_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP88161.1"
                     /translation="MTAPNPLETLTAVPPFYLGRQPILDRDGHTVAYELLFRPDRQNG
                     VVVTDDRAATASVIAHAFTDLGIQSVLGERDGLINFDAELLLSDIPELLPAARIVIEI
                     LETVVFTPEIMARCRDLKQQGFRLALDDVSQFEQLAPEVLELIDIVKVDLRAISMYQL
                     PKLAKAAKAARVTLLAEKVESLFLADRCRELGFDLFQGYYFARPMLLEGRRSQPDRHL
                     LLQLLQQVANDT"
     gene            complement(775..1488)
                     /locus_tag="PLS34_11690"
     CDS             complement(775..1488)
                     /locus_tag="PLS34_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88162.1"
                     /translation="MPTSKNRRRKPKRKASRPGTHRPHVAMARPPLGRGPGPGTEADV
                     RAVLNRHDCKIPYHTVRARFMGAIATPTPNVHPLAVVQSLWGGELPECRDLEEAQALL
                     DTLVMGLWNRLSIHQEPSRPFRLARLEPPNSSDSITPYVTTRIEELEAFVEGLFGGDN
                     AMDLPESAHEALGVLRDFLSFFYGYRQFAAEITTPAKLAEAALRMRQLTEASEAEIHT
                     IIRSCTRARRAALGGGAQP"
     gene            1618..2958
                     /locus_tag="PLS34_11695"
     CDS             1618..2958
                     /locus_tag="PLS34_11695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRP88163.1"
                     /translation="MKITIFGSGYVGLVTGACLADVGNQVVCVDIDDAKVDRLNAGEI
                     PIYEPGLDAVVARNREAGRLKFTTDVTQGVAHGLFQFIAVGTPPDEDGSADLQHVLAV
                     ARSIGEHMEEYRIVVDKSTVPVGTADKVKAEIERTLAAREAVIDFDVVSNPEFLKEGA
                     AVEDFMRPDRIIVGTDNPRTTELLRLLYEPFNRQHDRLIAMDVRSAELTKYAANAMLA
                     TKISFMNELANLAERFGADIEHVRLGIGSDPRIGYHFIYPGAGYGGSCFPKDVQALAH
                     SARKAGFPATLLEAVETVNARQKQVLFDKISAHYGGKLAGKTVALWGLAFKPNTDDMR
                     EASSRVLMESLWEAGATVRAYDPQARKETRRIYGERPDLVLCKNSAEALEGADCLALV
                     TEWMEFRSPDFDALRSALKDPVIFDGRNVFDPDFMKRLGFTYYAIGRGDRVGPA"
BASE COUNT          500 a          909 c         1047 g          507 t
ORIGIN      
        1 cgtgtcgttc gcgacctgct gcaggagctg cagcaacagg tggcggtcgg gctggctgcg
       61 gcgaccctcg agcagcatgg ggcgcgcgaa gtaatagccc tggaagaggt cgaagcccag
      121 ctcgcggcag cggtcggcga gaaacaggct ttccactttc tcagccagca gtgtaactct
      181 ggcggccttg gccgccttcg ccagcttggg cagctggtac atggagatgg cgcgaaggtc
      241 gaccttgacg atgtcgatga gctccagcac ctctggggcg agctgttcga actggctgac
      301 gtcgtcgagc gcgaggcgga atccctgctg cttgaggtcc cggcaacgcg ccatgatctc
      361 cggggtgaac acgacggtct cgagaatctc gatgacgata cgtgcggcgg gcagcagctc
      421 gggaatgtcc gacagcagca gctcggcatc gaaattgatt agcccgtcgc gctcgccgag
      481 tacggattgg atgccgagat ccgtgaaggc gtgggcgatc accgaggccg tggcggcgcg
      541 gtcgtcggtg accacgacgc cgttctgccg gtcgggccgg aacagcagct cataggcaac
      601 ggtgtggccg tcccgatcaa ggatgggctg gcgtccgaga tagaagggcg gcacggcggt
      661 cagcgtttcc agcgggttcg gcgcggtcat gctgcgtagc ttaatgaggt ccgctgggcg
      721 actggtggaa ccgcgtcgca aaatcaagtt tgcgtcgcgg gcggcagggg gccttcaggg
      781 ctgcgcgccc cctcccagcg ccgcgcgacg ggcgcgagta cagctgcgga tgatggtgtg
      841 gatttcggct tcgctggcct cagtgagctg gcgcatgcgc aaggccgctt ccgccagctt
      901 cgcaggcgtg gtgatttccg cggcgaattg ccggtagccg tagaagaagg acagaaagtc
      961 ccgaagcacg ccgagcgctt cgtgggccga ctcgggcagg tccatcgcgt tgtcgccgcc
     1021 gaacaagcct tcgacgaagg cctcgagttc ctcgatccgc gtggtcacgt agggtgtgat
     1081 gctgtccgag ctgttcggcg gttcgagacg cgcaaggcgg aacgggcggc tgggttcctg
     1141 gtggatggac agccgattcc acaatcccat caccagcgtg tcgaggagtg cctgggcttc
     1201 ctcgaggtcg cggcactcag gcaactcgcc gccccagagc gactgcacga ccgcaagcgg
     1261 gtggacgttc ggtgtcggcg ttgcaatggc acccatgaag cgcgcacgca ccgtgtggta
     1321 ggggattttg cagtcatgtc ggttgagcac tgcgcggacg tcggcttcgg tgcccgggcc
     1381 cgggccgcgt ccgagcggcg gccgggccat cgcgacgtgt ggccggtggg tgccgggacg
     1441 cgaggccttg cgctttggct tgcgacggcg gttcttcgag gtgggcatga cgctaaggat
     1501 agcgaaatcg gcgccttttc tgtgacctga ggccgtaatg ggtggggtat ttctgccata
     1561 atttcccgcc tgtcagttac ttgtcggggt ccgacccgga cgtaaaggga tattgcggtg
     1621 aaaattacga tttttggctc tggctatgtg ggactggtga cgggcgcctg cctggcggac
     1681 gtgggcaacc aggtggtctg cgtggacatc gacgacgcca aggtcgaccg cctcaatgcc
     1741 ggcgagatcc cgatctacga gccggggctg gacgccgttg tggcgcgcaa ccgcgaggcc
     1801 ggccgactga agttcaccac tgacgtgacg cagggcgtag ctcacgggct gttccagttc
     1861 atcgccgtcg gcacgccgcc ggacgaggat ggctcggccg acctgcagca cgtgctggcg
     1921 gtggcgcgct ccatcggcga gcacatggag gagtaccgca tcgtcgtgga caagtcgacg
     1981 gtgccggtcg gcaccgccga caaggtgaag gcggagatcg agcgcacgct ggccgcgcgt
     2041 gaagccgtca tcgatttcga cgtggtctcg aacccggaat tcctcaagga aggcgcggcg
     2101 gtcgaggact tcatgcgtcc ggaccgtatc atcgtcggca ccgacaaccc gcgcaccacc
     2161 gagctcttgc ggctgctcta cgaacctttc aatcgccagc acgaccggct catcgcgatg
     2221 gatgtccgct ccgccgagct gaccaagtac gcggccaacg ccatgctggc caccaagatc
     2281 agcttcatga acgagctggc caacctggcg gagcgcttcg gcgccgacat cgagcacgtg
     2341 cggctcggca tcggctccga cccgcgcatc ggctatcact tcatctaccc gggtgcgggc
     2401 tacggcggct cctgtttccc caaggacgtg caggcgctgg cgcactcggc gcgcaaggcc
     2461 ggcttcccgg ccaccttgct ggaagccgtc gagacggtga acgcgcgcca gaagcaggtg
     2521 ctgttcgaca agatcagcgc acactacggt ggcaagctgg cgggcaagac cgtcgcgctg
     2581 tgggggctgg cgttcaagcc gaacaccgac gacatgcgcg aggcgtccag ccgcgtgctg
     2641 atggagtcgc tgtgggaggc cggggcgacg gtgcgcgcct acgacccgca ggcccgcaag
     2701 gagacgcgcc gcatctacgg ggaacgtccc gacctggtgt tgtgcaagaa ctccgccgag
     2761 gcgctcgagg gggcggactg cctggcgctg gtgaccgagt ggatggagtt ccgcagcccg
     2821 gacttcgacg cgctcaggtc tgcgctgaag gacccggtca tcttcgacgg ccgcaacgtc
     2881 ttcgaccccg atttcatgaa gcgcctcggc ttcacctact acgccatcgg ccgcggcgac
     2941 cgggtcggac ccgcgtaagc aac
//
LOCUS       DAOMPG010000270         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13537_length_2961_cov_3.199587, whole genome shotgun sequence.
ACCESSION   DAOMPG010000270 DAOMPG010000000
VERSION     DAOMPG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13537_length_2961_cov_3.199587"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLS34_11700"
     CDS             complement(<1..687)
                     /locus_tag="PLS34_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP88164.1"
                     /translation="MERHQSIVEALRAALGSDAVLSDPADRWTYGYDNSRGQAMPLAV
                     ALPSDTAGVRRVVQTCRRFRVPLTVRGRGTGTTGAAVPEEDGVVLSMERMAKVISVDA
                     ANRVAVVQPGVTNGALQAAAGAAGFFWPPDPGSAAYCTVGGNLACNAAGPRALKYGTP
                     RDNTLGLEAVTGEGRVIRAGCYTTKGVVGYDLTRLLIGSEGTLAIITEATLKLTPLPE
                     AVATVRAAYTD"
     gene            complement(703..1065)
                     /locus_tag="PLS34_11705"
     CDS             complement(703..1065)
                     /locus_tag="PLS34_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRP88165.1"
                     /translation="MNGDRGRAAEDAALRFLESRGFALLCRNYRCRLGEIDLVMRDGA
                     SLVFVEVRARDSEAFGGAAASVGRAKQRRLAAAARHFLMTHPGEARRPARFDVVAISG
                     HDGENPPRWIRAAFDCGS"
     gene            complement(1062..>2961)
                     /locus_tag="PLS34_11710"
     CDS             complement(1062..>2961)
                     /locus_tag="PLS34_11710"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRP88166.1"
                     /translation="KLPRMLQLPHRTAQTLSAVALSVLLAGCTPGLLDRSAAEYEVQA
                     ASARQHQSAGDWATAAEAWLRAAEAARGEARDMPVAAAEAWLRAGEPERARDALGGLS
                     ANPGAELAARRTLIEARLLLRSDRAGQALARLAGVPMGAGDPMAAELLAVRAAAAFAA
                     RQPAMGVAALAQREALLTDPERRSANQRRIWSRMQEAAAAGLPLQAPAGADPVVAAWL
                     ELGREAAATRGNPFRLRAALAAWRERYPEHPAARDLLSTLLDEYRAMTEYPQRVALLL
                     PLGGRHGGAGGVVRDGFIAAYLAQGDEGERPALRIYDTTALGATAAYDLAARDGADFI
                     VGPLLKEDLAELAGAELPAVPGLVLNWPDEGLALPSYLFQFALAPEEEAAAAARRAIQ
                     DGHRRAVALVHDTEQGRRMAQSFISAFQAEGGEVLGWQVFETRAADFSVEITGLLLLN
                     ESRARYQRLQGVLGRNLEYVPRRRQDVDFIFLAARPAEALLIRPQLRFHYAGNLPIYA
                     TSSIYDPARRDNAELDGVLIADMPWRVGAGDGEFMARFGSFGPNALERNGRLYAFGAD
                     AYRLVPLLHNRSETLGTGVAGATGLLRVGADGRVHRELDWGRFEGGQVRHAPLAAPVV
                     EESAPPES"
BASE COUNT          402 a         1096 c         1071 g          392 t
ORIGIN      
        1 gtcggtgtag gcggcacgca ccgttgcgac cgcctccggc agcggcgtca gcttgagcgt
       61 cgcctcggtg atgatggcca gcgtcccctc ggagccgatc agcagccggg tcaggtcgta
      121 gccgaccacg cccttggtgg tgtagcagcc ggcgcgaatg acgcggccct cgccggtgac
      181 cgcttcgagc cccaaggtgt tgtcgcgcgg cgtgccgtac ttcagcgcgc gcggccccgc
      241 ggcgttgcag gcgagatttc cgcccaccgt gcagtacgcc gccgagcccg ggtcgggggg
      301 ccagaagaag ccggcggcgc cggccgccgc ctgcaacgcg ccattggtga cgcccggctg
      361 caccacggcg acgcggttgg ccgcatccac gctgattact ttcgccatgc gctccatcga
      421 gagcacgacc ccgtcttctt ccgggaccgc ggcgccggtc gtcccggtgc cgcgtccgcg
      481 caccgtcagg gggacgcgga agcggcgaca tgtctgcacg acgcgccgca cgcccgcagt
      541 gtcgctcggc agggcgaccg ccagcggcat ggcttgtccc cggctgttgt catagccgta
      601 ggtccagcga tcggcggggt cgctgagcac ggcatcgctg ccaagcgcgg cgcgcagcgc
      661 ctccacgatg ctctggtgtc gttccatggg ccgtccgttg actcaggagc cgcagtcgaa
      721 ggcggcgcgg atccaacggg gtggattctc gccgtcgtgc ccggagattg ccaccacgtc
      781 gaagcgcgcc ggtcggcggg cctcccccgg atgcgtcatg agaaagtgtc gcgccgccgc
      841 ggccagacgc cgctgcttgg cgcgccccac gctggcggcg gccccgccga aggcctcgct
      901 gtcgcgcgcc ctcacttcga cgaacaccag cgaggcgccg tcgcgcatca ccaggtcgat
      961 ttcgccgagc cggcagcggt agttacggca cagcagcgca aagccccggg attcgaggaa
     1021 gcggagtgcc gcatcctccg cggcacggcc gcggtcgccg ttcatgattc cggcggtgcg
     1081 gactcttcca ccacgggcgc cgccagcggc gcatgccgca cctgtccgcc ctcgaaacgc
     1141 ccccagtcga gttcgcgatg cacgcggcca tctgcgccga cgcgcagcag gccggtggcc
     1201 cccgccacgc cggtgccgag cgtttcgctg cgattgtgga gcagcggcac gagccggtag
     1261 gcgtccgcgc cgaaggcata caggcggccg ttgcgctcga gggcgttggg gccgaagctg
     1321 ccgaagcgcg ccatgaactc gccgtcacct gcgccgacgc gccacggcat gtcggcgatc
     1381 agcacaccgt cgagctcggc gttgtcgcgc cgcgccggat cgtagatcga cgacgtggca
     1441 tagatcggca ggttgccggc gtaatggaag cgcagctggg gacgaatcaa cagcgcctcg
     1501 gcgggccggg ccgccagaaa aatgaaatcc acgtcctggc ggcggcgcgg tacgtattcg
     1561 aggttccggc cgagcacgcc ctgcaggcgc tggtagcggg cccggctctc gttcagcagc
     1621 agcagcccgg tgatttccac cgagaagtcc gcggcacggg tctcgaacac ctgccagccg
     1681 agcacttcgc cgccttctgc ctggaaggcc gagatgaagc tctgcgccat gcggcgcccc
     1741 tgctcggtgt cgtgcaccag cgccacggcg cgccgatgac cgtcctggat cgcgcgccgg
     1801 gccgcggcgg cggcttcctc ttcgggcgcc agcgcgaact ggaacaggta tgaaggcagg
     1861 gcaaggcctt cgtcgggcca gttcagcacc agccccggca cggccggcag ctccgcgccg
     1921 gccagctcgg ccaggtcctc cttgagcaac ggcccgacga tgaaatcggc cccgtcgcgc
     1981 gcggcgaggt cataggccgc cgtcgcaccc agggcggtgg tgtcgtagat gcgcagcgca
     2041 gggcgctcgc cctcgtcgcc ctgcgcgagg taggccgcaa tgaagccgtc ccgtaccacg
     2101 ccgccggcgc caccgtgccg tccgccgagc ggcagcagca aggccacgcg ctgggggtac
     2161 tcggtcatgg cgcggtactc gtcgaggagc gtgctgagca ggtcgcgggc cgccggatgt
     2221 tccgggtatc gctcacgcca ggcggcaagg gcggcgcgca gccggaacgg attgccgcgg
     2281 gtcgccgcgg cctcgcgccc gagttcgagc caggcggcga cgaccgggtc cgcccccgcg
     2341 ggcgcctgga gcggcaggcc cgcggccgcg gcctcctgca tgcggctcca gatgcgccgc
     2401 tgattggcgc tgcgacgctc gggatcagtg agcagcgctt cccgttgcgc cagggcggcg
     2461 acgcccatgg ccggctgccg ggcggcgaag gccgcggcgg cacgcactgc gagcagttcg
     2521 gccgccatgg gatcgccggc ccccatgggg actccggcca gtcgcgccaa cgcctgcccg
     2581 gcgcggtccg accgcagcaa caggcgcgcc tcgatgagcg tgcggcgtgc cgccagctcg
     2641 gcgcccgggt tggcagacag cccgcccagc gcgtcgcgtg cgcgctccgg ctcgcccgcc
     2701 cgcagccagg cctcggccgc cgccaccggc atgtcgcgcg cctcgccgcg cgccgcctcg
     2761 gcggcccgca gccaggcctc ggcggccgtc gcccagtcgc cggccgactg gtgctgccga
     2821 gcgctggcag cctggacctc gtactcggcc gcgctgcggt cgagcaaccc gggcgtgcac
     2881 cccgccagca gcaccgacag ggcgacggcc gacagggttt gcgctgtgcg atgcggaagt
     2941 tgcagcatgc gagggagttt c
//
LOCUS       DAOMPG010000271         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13548_length_2959_cov_4.196281, whole genome shotgun sequence.
ACCESSION   DAOMPG010000271 DAOMPG010000000
VERSION     DAOMPG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13548_length_2959_cov_4.196281"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLS34_11715"
     CDS             <1..226
                     /locus_tag="PLS34_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88167.1"
                     /translation="VRESSVWFVVPTYGFMLGVFGMALWGLYRYLVDDLPQAQESLGE
                     EDWLALMKSWWGEASAEERREFLRWVGERR"
     gene            401..1444
                     /locus_tag="PLS34_11720"
     CDS             401..1444
                     /locus_tag="PLS34_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRP88168.1"
                     /translation="MKKTMLAIAIGATCFASAAASAAPTVYGRFNVALDSQKDEIGLD
                     FGTSDSTIKLRDNNNSSRLGVKGKEDLGVGGLGIVYQMEYGIDPDGSESNPFSKRNLF
                     VGLEGGFGQLKAGFYDTIIKDIGGPVDQFNDTVGDITNLMVGETRTSNLITYTSPKVG
                     EAVRFVVTLQPGEGRVAADDISDVEKSIADSWYAAMLYDGKMFSGAVAYGSNQRSGMK
                     FDGPTVGSDILRFTGKVKLDAFEIGALYQMAEGIDQTGGVAGSDAQEDSWLLSGAYSI
                     NSWKLKAQYGQTDGDLSGAKRTQMAVGADYKLSKATTTQLYYVTYEDKDRVVGGITDP
                     KTDTFGVAFIYSF"
     gene            1570..2286
                     /gene="phoU"
                     /locus_tag="PLS34_11725"
     CDS             1570..2286
                     /gene="phoU"
                     /locus_tag="PLS34_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRP88169.1"
                     /translation="MEHQDLSHHWSRQFNAELEAVRNSVMAMGGLVEEQLRRAVEALV
                     RGDSALGELVARDDYKVNRFEVSIDEDCSRIIARRQPTAGDLRLLMAVVKTITDLERI
                     GDEAEKIGYLASRLAGQERPSDSYQGIQHLADQVGAMLHRALDAFARMDPEEAMRVVR
                     QDEVVDREYDAVSRQGITLMMENPRSIRRVLDTLWVARALERIGDHAKNICEYVVYMV
                     RGKDIRHVALDDAERQIRNE"
     gene            2279..2785
                     /locus_tag="PLS34_11730"
     CDS             2279..2785
                     /locus_tag="PLS34_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRP88170.1"
                     /translation="MNETRTRGWLDRRMVNGYCALACGGLLVYALYAQHGLGLEPCPL
                     CVFQRVAVLALGFVFLAAYAHNPARGGASVYGTLAVVAAGGGIAFAGRQLWLQGLPAD
                     QVPECGPGLNFIMDVFPFWEAVKMVFQGSGDCAKIDWTFLGISMAGWVLISLVGLASI
                     AAWNNFRR"
BASE COUNT          497 a          981 c         1000 g          481 t
ORIGIN      
        1 ggtccgggag agctcggtgt ggttcgtcgt gcccacctac gggttcatgc tcggcgtgtt
       61 cggcatggcg ctgtgggggc tctaccgcta cctcgtcgac gacctgccgc aggcgcagga
      121 atcgctgggc gaagaggact ggctggccct gatgaagagc tggtggggcg aagcgagcgc
      181 cgaggagcgg cgcgagtttc tccgctgggt cggcgagcgg cgctgacgca ccgccgcggg
      241 cgcagcacgc gtccgacatc gtcccggctt gcgggcggcc gggcccgtcg tccctcgctc
      301 gcgtcgcgtt tcgtcacgaa accttcacag tcgatctcca tacttcgcgc cgattgcgga
      361 aggcgtgact tctgcattgt gacaacccaa ggagattcct gtgaagaaga ccatgcttgc
      421 gattgcgatc ggcgcgacct gcttcgcgtc ggctgccgct tccgccgcgc cgaccgtata
      481 tggccgcttc aacgtggcgc tggacagcca gaaagacgag atcggcctcg acttcggtac
      541 gtccgattcg accatcaagt tgcgcgacaa caacaactcc tcgcgtctcg gcgtgaaggg
      601 caaggaagac ctcggtgtcg gcggcctcgg catcgtttac cagatggagt acggcatcga
      661 cccggacggc agcgagtcca acccgttctc caagcgcaac ctgttcgtcg gcctcgaggg
      721 cggtttcggc cagctgaagg ccggtttcta cgacaccatc atcaaggaca tcggcggccc
      781 ggttgaccag ttcaacgaca ccgtcggcga catcaccaac ctgatggtcg gcgagacccg
      841 caccagcaac ctgatcacct acacctcgcc caaggttggt gaagcggtgc gcttcgtggt
      901 gacgctgcag ccgggcgagg gccgtgtggc ggccgacgac atcagcgacg tcgagaagag
      961 catcgccgat tcctggtacg ccgccatgtt gtacgacggc aagatgttca gcggtgccgt
     1021 ggcctacggc agcaaccagc gcagcggcat gaagttcgac ggccccacgg tcggcagcga
     1081 catcctgcgc ttcaccggca aggtcaagct cgacgccttc gagatcggcg ccctgtacca
     1141 gatggccgag ggcatcgacc agaccggcgg cgtggcgggc agcgacgcgc aggaagacag
     1201 ctggctgctg tcgggcgcct actcgatcaa ttcatggaag ctgaaggccc agtacggcca
     1261 gacagacggc gatctcagcg gcgcgaagcg cacccagatg gcggtcggcg ccgactacaa
     1321 gctgagcaag gccaccacga cccagctgta ctacgtcacc tacgaggaca aggaccgcgt
     1381 cgtgggtggc atcaccgatc ccaagaccga caccttcggc gtggcgttca tctacagctt
     1441 ctgagctccg gccctcccct tgaaggccac tggaagggcc cggcaactgc cgggcctttt
     1501 cttttgaccg actcggcgcc cgggtgcgaa catggaaccg gcgccgcccg gcgctgtccg
     1561 agtgaatcga tggaacacca ggatctcagt caccactggt cgcgccagtt caacgccgag
     1621 ctcgaggccg tgcgcaacag cgtcatggcg atgggcgggc tggtcgagga acagctgcgg
     1681 cgtgccgtgg aggcgctggt gcgcggcgac agcgcgctcg gcgaactggt ggcgcgcgac
     1741 gactacaagg tcaaccgctt cgaggtctcc atcgacgagg actgcagccg catcatcgcg
     1801 cgccgccagc cgacggccgg cgacctccgg ctgctcatgg cggtggtcaa gaccatcacc
     1861 gacctggaac ggatcggcga cgaggccgag aagatcggct atcttgcttc gcgcctcgcc
     1921 ggccaggagc ggccgtccga cagctaccag ggcatccagc acctggccga ccaggtcggc
     1981 gcgatgctgc accgggcgct ggacgccttc gcgcggatgg acccggagga ggccatgcgg
     2041 gtggtgcgcc aggacgaggt ggtggatcgc gaatacgacg ccgtttcgcg ccagggcatt
     2101 acgctgatga tggagaaccc gcgcagcatc cggcgggtgc tggacaccct gtgggtggca
     2161 cgcgcgctgg agcgcatcgg cgaccatgcc aagaacattt gtgaatacgt cgtctacatg
     2221 gtgcgcggca aggacatccg ccacgtggcc ctcgacgacg cggagcggca gatcaggaat
     2281 gaatgagaca cgaactcgcg gctggctcga ccgccgcatg gtgaacggtt actgcgcgct
     2341 ggcctgcggc ggcctgctcg tctacgcgct gtacgcgcag cacggcctcg ggctcgagcc
     2401 ctgcccgctg tgcgtgttcc agcgcgtcgc ggtgctggcg ctcggcttcg tgttcctcgc
     2461 ggcctacgcg cacaacccgg cccgcggcgg ggcgagcgtg tacggcacgc tcgcagtggt
     2521 cgccgccggg ggcggcatcg ccttcgccgg gcgccagctc tggctgcagg gcctgcccgc
     2581 cgaccaggtg ccggagtgcg gacccggcct gaatttcatc atggacgtgt tccccttctg
     2641 ggaggccgtc aagatggtgt tccagggctc gggcgactgc gccaagatcg actggacctt
     2701 tctcggcatc agcatggccg gctgggtgct gatctcgctg gtcgggctcg cctcgatcgc
     2761 cgcctggaac aacttccgcc gctaaaccgg ggtcagacac ctatttccgg ttgtccgcgg
     2821 caccgtgccg tcatgggcgc gctgcggttg ccgctcgacc gcgggaccgt gccgccacag
     2881 gcgcgcttcg ctcggcgaaa tctttagctc gctcagcgcg cccatgacgt cccggtcccg
     2941 ctgccgtgcc gacaagggc
//
LOCUS       DAOMPG010000272         2952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13592_length_2952_cov_3.995513, whole genome shotgun sequence.
ACCESSION   DAOMPG010000272 DAOMPG010000000
VERSION     DAOMPG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13592_length_2952_cov_3.995513"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..849
                     /locus_tag="PLS34_11735"
     CDS             154..849
                     /locus_tag="PLS34_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRP88171.1"
                     /translation="MAQRPVTERWLFSCEHGGCEVPDEYRSVFRDAREVLDSHRGWDA
                     GALEVFRAISPGLAEAAFAATTTRLLIDLNRSLHHPRVFSEFTRPLPRAARDEIVACW
                     WRPWREAVAGTVAGSLEGGHLVRHISVHSFTPVLHGSVRNADIGLLYDPARARERSFC
                     LRWQSLLQARGWRVRRNYPYLGVADGHATALRRRFGPGYAGIELEMNQALFPARTGPL
                     CADLDATLRELRG"
     gene            complement(891..1271)
                     /locus_tag="PLS34_11740"
     CDS             complement(891..1271)
                     /locus_tag="PLS34_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRP88172.1"
                     /translation="MSSTAMFWPMLGMAVLTAVAWVVMGRRRQAAVRSHTMRLRDFAG
                     TGDIPMTPEVSVSTRHFANHFEIPTLFHIVCLAHIALAAASWAALALAWGFVVLRALH
                     MREHLGRNRVMTRFNLYASATTLM"
     gene            1458..2348
                     /locus_tag="PLS34_11745"
     CDS             1458..2348
                     /locus_tag="PLS34_11745"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRP88173.1"
                     /translation="MLAAGLAVAGTVAASDAVVSADWLAERLDDRNIRIVEVSVEPGK
                     FERAHIPGAVNFRWHTDLVDPVRRDIASRDNWEQLLSAAGIGPDTTVVLYGDHNNWFA
                     AWGAWIFRYYGHADVKLLDGGRNKWEADKRPLGSRPTRHAATEYRVTAENPALRARLA
                     DILEVVEGERRAKLLDIRSPAEYEGKIFAPPGVQELAIRAGHIPGAKNVPWSSVVRED
                     GTFKSADELRKLYAGIGIDGSEPVIVYCRIGERSAHSWFVLSQILGYEAVQYDGSWTE
                     YGNAVGVPVSNPAGTVWGGT"
     gene            2399..>2952
                     /locus_tag="PLS34_11750"
     CDS             2399..>2952
                     /locus_tag="PLS34_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRP88174.1"
                     /translation="MFVFAARLDGHAALGRDPAFTVLAGAVFGVLLQRARFCFFCVLR
                     ELFEERDARGALGILAALAVGSIGYLVVMQPWVPDPSAGRLPPDAHIGPVSWVLVAGG
                     LSFGLGMTLSGSCISAHLYRLGEGSTLAPFALLGAVGGFMLGFVSWNWLYIGTLATAP
                     VVWLPASLGYGGAAGLQAALLLGLA"
BASE COUNT          410 a          999 c         1050 g          493 t
ORIGIN      
        1 cccggcgcac gcggcgctgg cgcgtatcct ggacgagggt ctgctggcgc ggcggctgct
       61 ccgcgccgtc ggcagctcgc cccggcgcgc gaagttggtc gcggcatgcc gcgaactggc
      121 gcagtgcctg gcggacaacc ggctgttccc ctgatggcgc agcggccggt gacggagcgc
      181 tggctgttca gctgtgaaca cggcggctgc gaggtgccgg atgaataccg gtccgtgttc
      241 cgcgatgccc gcgaggtgct ggactcgcat cgcggctggg acgcgggcgc cctcgaagtg
      301 ttccgggcga tctcgccggg gctggccgag gccgccttcg ccgccaccac cacccgcctg
      361 ctgatcgacc tgaaccgctc gctgcatcat ccgcgcgtgt tctccgagtt cacccggccc
      421 ctgccgcgag cggcgcgcga cgagatcgtg gcgtgctggt ggcgtccctg gcgcgaggcg
      481 gtggcgggca ccgtggccgg gtcgctcgag ggcggtcatc tcgtccggca catttcagtg
      541 cacagcttca ctccggtgtt gcacggcagc gtgcggaacg ccgacatcgg gctgctgtac
      601 gacccggcgc gcgcacgcga gcggagcttc tgcctgcggt ggcagtcgct gctgcaggcg
      661 cgcggctgga gggtgcgccg gaactatccc tacctcggcg ttgcggacgg ccacgccacg
      721 gcgctgcgcc ggcgcttcgg gccaggctat gccggcatcg agctggaaat gaaccaggcg
      781 ctcttccccg cccggaccgg tccactgtgc gcggacctcg acgcgaccct gcgcgagctc
      841 cggggctagg cgcggagcag gaccacgacg gtcacgtgca gtcagagcaa ctacatcagc
      901 gtggtggcgc tggcgtacag gttgaagcgc gtcatcaccc ggttccgccc gaggtgctcg
      961 cgcatgtgca atgcgcgcag taccacgaag ccccacgcca gcgcgagcgc cgcccagctg
     1021 gcggctgcca gcgcgatgtg cgccaggcag acgatgtgga acagggtggg aatctcgaag
     1081 tgattggcga agtgccgcgt tgagacggac acctcgggcg tcatgggaat gtcgcccgtg
     1141 ccggcaaaat ccctgagccg catcgtgtgg ctgcggacgg ccgcctggcg ccggcggccc
     1201 atgaccaccc aggcgacggc cgtgagtacc gccatgccga gcatcggcca gaacatcgct
     1261 gttgaagaca tggtcgcctc ccgggatcta ggggttgcat cacaaccttg gggaatgagc
     1321 ctatcccgaa aagttgttgg acggcatagc agccgcatcc tatatttttt tccccgcttc
     1381 ggggccgttt ccgcgcaaga actattgagg tgagtgacgt gaatatcagc aagttacgat
     1441 caacagccgg cgccgttctg ctggccgccg ggctggccgt ggccggcacg gtcgccgcct
     1501 cggacgccgt ggtcagcgcc gactggctcg ccgagcgact ggatgacagg aacatccgca
     1561 tcgtggaggt cagcgtcgag ccgggcaagt tcgagcgcgc ccatatcccc ggcgcggtca
     1621 atttccgctg gcataccgac ctggtcgacc cggtgcgccg cgacatcgct tcgcgggaca
     1681 actgggagca gctgctgtcg gccgcgggca tcggccccga cacgacggtg gtgctgtacg
     1741 gcgaccacaa caactggttc gctgcctggg gtgcgtggat cttccgctac tacggccacg
     1801 ccgacgtcaa gctgctcgac ggcggccgta acaagtggga agccgacaag cggccgctcg
     1861 gcagccgtcc gacgcgccat gccgccaccg aataccgggt cacggccgag aaccccgcgc
     1921 tgcgcgcccg cctcgccgac atcctcgagg tggtcgaggg cgagcgtcgc gccaagttgc
     1981 tggacatccg ctcgccggcg gaatacgagg gcaagatctt cgctccaccg ggggtgcagg
     2041 aactggccat ccgcgccggc cacatacctg gcgcgaagaa cgtcccatgg agctcggtgg
     2101 tgcgcgagga cggcaccttc aagtcggccg acgagctgcg caagctctat gcaggcatcg
     2161 gcatcgatgg cagcgagccg gtgatcgtct attgccgcat cggcgagcgc tccgcgcaca
     2221 gctggttcgt gctgtcgcag atcctcggct acgaggccgt gcagtacgac ggctcctgga
     2281 cggagtacgg caatgccgtc ggcgtgccgg tctcgaaccc ggccggcacg gtgtggggcg
     2341 gtacctgagt cccggcctgc tgattcgcat cctggtgccg gcgacgctcg tcgccggcat
     2401 cttcgttttt gccgctcgcc tggacgggca cgccgcgctg gggcgcgatc ccgccttcac
     2461 cgtgctggcc ggggcggtct tcggcgtgct gctgcaacgc gcgcgctttt gttttttctg
     2521 cgtcctgcgt gagttgttcg aggagcgcga cgcgcgtggc gcgctcggca tccttgccgc
     2581 cctcgcggtg ggcagcatcg gctacctcgt cgtgatgcag ccctgggtgc ccgacccgtc
     2641 ggccggacgc ctgccgcccg acgcccacat cggaccggtc tcgtgggtgc tggtggccgg
     2701 cggcctcagt ttcggcctgg gcatgacgct gtcgggttcc tgcatctcgg cgcacctgta
     2761 ccggctcggc gagggctcga cactggcgcc gttcgccctg ctcggggcag tgggcggatt
     2821 catgctcggc ttcgtctcgt ggaactggct gtacatcggt acgctggcta cggcgccggt
     2881 ggtctggctg ccggcgagcc tcggttacgg cggggcggcg ggactgcagg ccgcgctgct
     2941 gctcgggctg gc
//
LOCUS       DAOMPG010000273         2938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13677_length_2938_cov_4.513007, whole genome shotgun sequence.
ACCESSION   DAOMPG010000273 DAOMPG010000000
VERSION     DAOMPG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_2938_cov_4.513007"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLS34_11755"
     CDS             complement(<1..166)
                     /locus_tag="PLS34_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRP88175.1"
                     /translation="MKDNYYEFFCPVKLIVGARALENIPFELGALGAQRPLVVTDRGV
                     AGAGLVDKLRA"
     gene            complement(168..692)
                     /locus_tag="PLS34_11760"
     CDS             complement(168..692)
                     /locus_tag="PLS34_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88176.1"
                     /translation="MRSFRDTLKRLYVAAMMRIVGWGLRAMSRVDPVVRAEAAGFPDG
                     MVIAMTTMPDGPSMVLQKIPGGPLRYLGGRLPAHTDLAIRFKHLDFAFRVFGFVESTP
                     TAFARARMVVDGDLAAGIRMVRCMYRLEALLMPRFIAARGMKRYPAELGLGEKLGMNL
                     KVYGRIALDLVTRS"
     gene            815..1111
                     /locus_tag="PLS34_11765"
     CDS             815..1111
                     /locus_tag="PLS34_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88177.1"
                     /translation="MGTDTLNCWRCGAEVVDEPLPLSRTAECRQCRAELHVCRMCEFY
                     DPTKANACREPVAEPVADKTRANFCGWFRPRPGLAPAGNAAAEEARNRLEDLFK"
     gene            complement(1104..1721)
                     /locus_tag="PLS34_11770"
     CDS             complement(1104..1721)
                     /locus_tag="PLS34_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRP88178.1"
                     /translation="MNPESRTATANDRRRTLLQRACLGLLRLFGWRALSAPLPGPKGV
                     IIVYPHTSNWDFILGLLYRFGTGLQANWMGKDAIFRWPFRRLMVKMGGVPINRRAASG
                     FIGAAIEQYRTQDWMWLALAPEGTRKHTDHLKSGFYQIAVGADVPCGLGFIDYGTRTL
                     GIQEFVRFSGVVEDDLATLKAFYANKRGRLPDDASEIRFRRQGPT"
     gene            1829..2305
                     /locus_tag="PLS34_11775"
     CDS             1829..2305
                     /locus_tag="PLS34_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88179.1"
                     /translation="MTTHVCRQCNADIQPHKLGRVEGEDGEIRITIDGLPMVECTNGH
                     KRFPSADFPLKFIETMLKSDDLVVAEPAVEKGLFRKHAHCPGCGQQLPEQSGGASAQR
                     VRVDMPQLDPVGVELAVPLFACPGCRKEATLPKNGVQRGVMQAVANAFRSADIPPG"
     gene            complement(2302..>2938)
                     /locus_tag="PLS34_11780"
     CDS             complement(2302..>2938)
                     /locus_tag="PLS34_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88180.1"
                     /translation="MPTHAARPGWSAGLRILLVAMALATFGCGSQQAEDAGHYRIVTV
                     KPSWLGGGCQPRTEFYFVDPQSPGAPLYLGTCGTPQFVTRHLGMPSDPSCFAVARDGS
                     ALVYFHRPNWCGAGETAANKPGGVYRHATAEGDALLYSDREHVTQVWTRGPIEPDAIR
                     VAWKNARPSRAGASCSQQLLIRADGSETPVGESTTVHGCPAGRDDAPAEGY"
BASE COUNT          465 a          974 c         1058 g          441 t
ORIGIN      
        1 tcgcgcgcag cttgtccacc aggccggcgc ccgccacgcc gcggtcggtg accaccagcg
       61 gccgctgcgc cccgagcgcg ccgagctcga agggaatgtt ctcgagggcc cgcgcgccga
      121 cgatcagctt cacggggcag aagaattcgt agtagttgtc cttcacgtca gctcctggtc
      181 acgagatcga gcgcgatgcg gccgtagacc ttcaggttca tgccgagctt ctcgccgagc
      241 cccagttcgg cggggtaacg cttcatgccg cgggcggcaa tgaagcgggg catcaagagc
      301 gcctcgagcc ggtacatgca gcgcaccatg cggatgcctg ccgcgagatc gccgtccacc
      361 accatgcgcg cgcgggcgaa ggccgtcggc gtgctctcga cgaagccgaa cacccggaag
      421 gcgaagtcga gatgcttgaa gcggatcgcc aggtcggtgt gcgcgggcag ccggccgccg
      481 aggtagcgca gcgggccgcc gggaatcttc tgcagcacca tgctggggcc gtccggcatg
      541 gtggtcatgg cgatcaccat gccgtcgggg aagccggccg cctcggcgcg caccaccggg
      601 tccacgcgac tcatggcgcg caggccccag ccgacgatgc gcatcatcgc ggccacgtag
      661 aggcgcttca gtgtgtcgcg gaagctgcgc atgggtggcc tcgctggggt tgattgcggc
      721 agggcagcat agtgtagcgc cggtgcgccg ccgcggggag atcgcgtcgg cgcgcgtcgc
      781 acccggcgct acaatggcag gcaggaggcg agccatgggc accgataccc tgaactgctg
      841 gcgctgcggc gcggaggtcg tggacgagcc gctgccgttg tcgcgcaccg ccgagtgccg
      901 ccagtgccgc gccgagctgc acgtctgccg gatgtgcgag ttctacgatc ccaccaaggc
      961 gaacgcctgc cgcgagccgg tggccgagcc ggtggcggac aagacgcgcg ccaatttctg
     1021 cggctggttc cggccgcgtc ccggccttgc gccggccggt aacgccgccg ccgaggaggc
     1081 gcgcaaccgg ttggaggacc tgttcaagta gggccctggc ggcggaagcg gatctcgctg
     1141 gcgtcgtcgg gcaggcggcc ccgcttgttc gcatagaagg cctttaacgt cgccaggtcg
     1201 tcctcgacca cgccgctgaa gcgcacgaat tcctggatgc ccagcgtccg cgtgccgtag
     1261 tcgatgaagc ccaggccgca gggcacgtcg gcgcccaccg cgatctggta gaagccggat
     1321 ttcaggtggt cggtgtgctt gcgggtgccc tccggggcga gcgccagcca catccagtcc
     1381 tgggtcctgt attgctcgat ggcggcgccg atgaagccgc tggccgcgcg ccggttgatg
     1441 ggcacgccgc ccatcttcac catcaggcgg cggaacggcc agcggaagat ggcgtccttg
     1501 cccatccagt tcgcctgcag gccggtgccg aagcgataca gcaggccgag gatgaagtcc
     1561 cagttcgagg tgtgcgggta gacgatgatg acgcccttcg ggcccggcag gggcgccgac
     1621 agggcccgcc agccgaacag gcgcagcagg ccgagacagg cgcgctgcag cagggtgcgg
     1681 cgtcggtcat tcgcggtggc ggtgcgggat tcggggttca tcgattggtg cgactccgtc
     1741 ggctgcgcta tcttgtgaca cgagcgccaa tgcagcaacc gcgtacggag gagctcagca
     1801 gacccggacc agggggagga gagccaaaat gacgactcac gtctgccggc aatgcaatgc
     1861 cgacatccag ccacacaagc ttggtcgcgt cgagggcgag gatggggaga ttcgcatcac
     1921 catcgacggc ctgccgatgg tcgagtgcac caatggccac aagcgtttcc cgagtgcgga
     1981 ttttccgctc aagttcatcg agacgatgct caagtcggac gatctcgtgg tcgccgagcc
     2041 ggccgttgag aagggcctgt tccgcaagca cgcccattgt ccgggctgtg gccagcaact
     2101 gccggaacag agcggcgggg ccagcgccca acgggtccgt gtcgacatgc cccagcttga
     2161 tccggtgggt gtcgaactgg cggtgccgct gtttgcctgt ccgggctgcc gcaaggaggc
     2221 caccctgccg aagaacggcg tgcagcgcgg cgtcatgcag gcggtcgcca acgcctttcg
     2281 cagcgccgac atcccgccgg gctagtaccc ttccgcaggc gcgtcgtcgc gtcccgccgg
     2341 gcagccgtgc acggtggtcg attccccgac gggcgtctcc gagccgtcgg cgcggatgag
     2401 aagttgctgc gagcagctcg cgccggcgcg gctcggacgc gcgttcttcc aggcgacccg
     2461 gattgcgtcg ggttcgatgg gcccgcgggt ccacacctgc gtgacgtgct cgcggtcaga
     2521 gtacagcagc gcatcgcctt ccgccgtggc gtggcggtag acgccgcccg gcttgttcgc
     2581 cgccgtctcg ccggcgccgc accagttcgg gcggtggaaa tagaccagcg cgctgccgtc
     2641 ccgtgcgacg gcaaagcagc tcgggtccga gggcatgcca agatgcctgg tgacgaactg
     2701 cggcgtgccg caggtgccga ggtacagggg cgcgccgggc gactgcgggt cgacgaaata
     2761 aaactcggtg cgcggctggc agccgccgcc gagccacgaa ggcttcactg tcacgatgcg
     2821 atagtggccg gcgtcctcgg cctgttgcga gccgcagccg aaagtcgcca gcgccatcgc
     2881 caccagcagg attcgcagtc cggctgacca cccggggcgc gcagcatgtg tgggcatc
//
LOCUS       DAOMPG010000274         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13714_length_2933_cov_3.549687, whole genome shotgun sequence.
ACCESSION   DAOMPG010000274 DAOMPG010000000
VERSION     DAOMPG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13714_length_2933_cov_3.549687"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1874
                     /gene="cydC"
                     /locus_tag="PLS34_11785"
     CDS             228..1874
                     /gene="cydC"
                     /locus_tag="PLS34_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HRP88181.1"
                     /translation="MSAGLPLWRLLGRRRGWLAAGVALVMLSLLAGVALLALSGWFIT
                     ASALAGLGLIAMLDIFAPGAGIRLAAVTRTVARYGERLVTHEATFRLLADLRLALFQK
                     LLALDEMQLRGLRRGDTLNRLTADVDTLDHLFLGVIGPGVSAFLLTALAALLAALWID
                     PGTVLVPTLVLLAVNPLVAELVRRRGRAASHALVAALPELRRHAGDGLEGLQELVAFD
                     LGDAQRARLEAESARLLGLQERLQRLDAAGQAGVTLAGLLASWLALALGLLLLQAGAI
                     SGPVLGLLVLGVLALGEAWQPLPGAWRRLEQCRGARARLDETWRQAPLLSVAARPLAP
                     AGRALSLEGVTFRYRNDLPPVLADFSLDITAGERIAVVGPSGAGKSTLAMLLMRQLDP
                     QAGTVRLAGVDLRTLDPDGLRRHIGLLAQRPVLFRDTLAENLRIARPAASDGELRHAL
                     EVAGLGDFVAGLADGLDTWIDEAGGNLSGGEARRVALARLALTDCPVVVLDEPTTGLD
                     AATAHGLAATLDGWLAGRTAVMITHDPGLLPRYDRLIRVG"
     gene            complement(1944..>2933)
                     /locus_tag="PLS34_11790"
     CDS             complement(1944..>2933)
                     /locus_tag="PLS34_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRP88182.1"
                     /translation="NSLNIPAPLLDRMEVIRIPGYTEDEKLNIAKRYLVPKQVKNNGL
                     KEEELAISDGAIRDIIRYYTREAGVRNLERELARICRKVVKEMVLDKDQKKVTVKPAG
                     IEKYLGVRRFRYGRAEETDRVGLATGLAWTEVGGELLTIEAATLPGKGKLIHTGTLGD
                     VMKESIQAAMTVVRSRSRVLGIDPEFYAKHDVHIHVPEGATPKDGPSAGIGMCTALVS
                     ALTGIPVRSDVAMTGEITLRGEVLPIGGLKEKLLAAHRGGITTVLIPEENEKDLAEIP
                     KNIKSKLDIKPVKWIDQVLEVALAHMPEPRADVTPEEPPATDVKPEVKGVHAH"
BASE COUNT          330 a         1045 c         1074 g          484 t
ORIGIN      
        1 cggtcgtcgc cgtagatgcg ggcatagggg atcacctcga tcccggcctc gtacctgatc
       61 gaccccgagg ggacgatcat ccggctggac cttcgcggtc ccgccctcga cgcggcgctg
      121 gcacagcagc ccatgaccgt ggtgtgcgcg agccacagcc cgctggcgct ggcatgggct
      181 gaccgctctg tcgaattggg gactgtcccc aattcggggg acggggcatg agcgccggcc
      241 tgccgctgtg gcgactgctc gggcggcggc gcggctggct ggccgccgga gtcgccctgg
      301 tcatgctcag cctgctggct ggtgtcgccc tgctggcgct gtcgggctgg ttcatcaccg
      361 ccagcgccct ggcggggctg ggcctgatcg caatgctgga catcttcgcg ccgggcgcgg
      421 gcatccgcct cgccgccgtc acgcgcacgg tggcccgcta cggcgagcgg ctggtgaccc
      481 acgaggccac ctttcggctg ctggccgacc tgcgcctcgc gctgttccag aagttgctgg
      541 cgctcgacga gatgcagctg cgcggcctgc ggcgcggcga cacgctcaat cggctgacgg
      601 ccgatgtgga cacgctggat cacctgttcc tgggcgtcat cgggccgggc gtgtcggcgt
      661 tcctgctgac cgcgctggcc gcactgctgg cggccttgtg gatcgatccg ggcacggtgc
      721 tggtgccgac gctggtgctg ctggccgtca acccgctggt ggccgagctg gtcaggcggc
      781 ggggccgggc cgcgagccac gccctggtcg cggcgttgcc cgagctcagg cgccatgccg
      841 gggacgggct ggaagggctg caggagctgg tggcgttcga cctcggcgac gcccagcggg
      901 cgcggctcga ggccgaatcg gcgcgcctgc tcggattgca ggagcgactg cagcggctgg
      961 acgccgccgg ccaggcgggc gtgacgctgg ccggcctgct ggcgagctgg ctggcgctgg
     1021 cgctggggct cctgctgctg caggccggcg cgatcagcgg cccggtgctg ggcctgctgg
     1081 tgctcggcgt gctggcgctg ggcgaggcct ggcagccgtt gccgggggcc tggcggcgcc
     1141 tcgaacaatg ccgcggcgcc cgggcccgcc tcgacgagac ctggcgacag gccccgctgc
     1201 tcagcgtcgc ggcgcgaccg ctcgcgcccg cggggcgggc cctcagcctg gaaggggtga
     1261 ccttccgtta ccgcaacgac ctgccgccgg tgctggcgga cttttcgctg gacattacgg
     1321 ccggtgagcg cattgccgtg gtcggaccga gcggcgccgg caagagcacg ctggcgatgc
     1381 tgctgatgcg acagctcgac ccgcaggcgg gcacggtacg actcgccggc gtcgacctgc
     1441 ggacactgga cccggacggc ctgcgccgcc acatcggcct gctggcgcag cggccggtgc
     1501 tgttccgcga caccctggcc gagaacctgc gcatcgcccg gccggcagcg agcgacggcg
     1561 aactgcgcca cgcgctcgaa gtcgcgggcc tgggggattt cgtcgccggg ctggccgacg
     1621 gcctcgacac ctggatcgac gaggccggcg gcaacctgtc gggtggcgag gcccggcggg
     1681 tcgccctggc gcgcctggcc ctgaccgact gcccggtggt agtgctcgac gagcccacca
     1741 ccgggctgga cgccgccacg gctcacggcc tggcggcgac cctggacggc tggctggcgg
     1801 gccgcaccgc cgtgatgatc acgcacgacc ccgggctgct gccgcgttac gaccggctga
     1861 tccgggtcgg ctgagccgcg cccggcggcg ccgggcgtcc cggtggttgg gccggccgtg
     1921 gtccggccgg cctgcgccgg cgctcagtgc gcgtggacgc ccttcacttc cggcttcacg
     1981 tccgtggccg gcggctcctc cggcgtcacg tccgcgcgcg gctcgggcat gtgcgccagc
     2041 gccacctcga gcacctggtc gatccacttg accggcttga tgtccagctt gctcttgatg
     2101 ttcttcggaa tctccgcgag gtccttctcg ttctcctccg ggatgagcac cgtggtgatg
     2161 ccgccgcggt gcgccgccag cagcttctcc ttgagcccgc cgatcggcag cacctcgcca
     2221 cgcagcgtga tttcgccggt catggccacg tcggagcgca ccggaatccc ggtcagcgcg
     2281 gagaccagcg ccgtgcacat gccgatgccg gcgctcgggc cgtccttcgg cgtcgcgcct
     2341 tccggcacgt ggatgtgcac gtcgtgcttg gcatagaact cggggtcgat gccgagcacg
     2401 cgcgagcggc tgcgcaccac ggtcatggcc gcctggatgg attccttcat cacgtcgccc
     2461 agcgtgccgg tgtggatcag cttgcccttg ccgggcagcg tcgccgcctc gatggtcaac
     2521 agctcgccgc cgacctcggt ccaggccagc ccggtggcca gcccgacgcg gtcggtctcc
     2581 tcggcgcgac cgtaacggaa gcgtcgcaca ccgaggtatt tctcgatgcc cgcgggcttc
     2641 acggtgacct tcttctggtc cttgtcgagc accatctcct tgacgacctt gcggcagatc
     2701 cgcgccagct cgcgctcgag gttgcggacg ccggcctcgc gcgtgtagta gcggatgatg
     2761 tcgcggatcg cgccgtcgct gatcgcgagc tcctcttcct tgagcccgtt gttcttgacc
     2821 tgcttcggca ccaggtagcg cttcgcaatg ttgagcttct cgtcctcggt gtagccgggg
     2881 atgcggatga cctccatccg gtccagcagc ggcgccggga tgttcaggct gtt
//
LOCUS       DAOMPG010000275         2926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13747_length_2926_cov_5.377569, whole genome shotgun sequence.
ACCESSION   DAOMPG010000275 DAOMPG010000000
VERSION     DAOMPG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13747_length_2926_cov_5.377569"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    362..437
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(581..2653)
                     /locus_tag="PLS34_11795"
     CDS             complement(581..2653)
                     /locus_tag="PLS34_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HRP88183.1"
                     /translation="MLAALAAASTLAAPAVAIAAAPSSPAAAVAGEWPRHGLDAAETR
                     FSPLSAIDTANVERLGLAWHFRFDRPRGVEATPIMVDGTLFVTGPWGVVYALDARSGQ
                     LKWQHDPEVPAGKGMHACCDVVNRGVAVEDGRVFVATIDGRLQALDARDGRLLWSTGT
                     FDIAAPYTITGAPRVARGLVVIGNGGAEYGVRGFVSAYDARTGALRWRFYTVPGNPAD
                     GADGAASDRPLETLARPTWTGEWWRQGGGGTVWDSMAWDPELDLLYFGVGNGSPHDRD
                     LRSPDGGDNLFLSSIVAVRRASGEYVWHYQTTPGDSWDYTATQHMILADLAVDGQPRK
                     VLMQAPKNGFFYVLDRATGELLSADKYVAVNWASHVDPASGRPVETAGARYPAGQPFL
                     VQPSQLGGHNWQPMAWSPETGLVYVPAQENAGFMMKEPAFRHEPERWNTGVLNPPMPP
                     DRAVLEQGKASMRGHLLAWDPVRRTAAWRTPHAGSWNGGVLATAGGLVFQGVGGEGLA
                     AFDARDGRRLWQSDTWLDILGGPISYTLDGEQYIAAAAGFGSSMHITSSALLPRTGAP
                     VPGGLFVWKLGGQAAMPEPSARPRVAPPPDTGTEAEVAHGAARYARYCMQCHGAGAVA
                     GGGMPDLRHSPYLSEAALFRRPLLDGLLAARGMPGFAQTLTTADADAIRHYIIRMAHT
                     LAQLPAKD"
BASE COUNT          408 a          976 c         1054 g          412 t
ORIGIN      
        1 cgacccgctg tacgtgcgcg acgaggcggg cgccacctac gtgccgtact cggcagaggc
       61 gctcgcacgg gcgggactgc cgccgttcgc ctgaggcggg gcagcaaacg atcgatcggc
      121 gctctgcgga gcgccgtttt tcttgattta gtccattcgg acgatgtcgc gcttgcgcac
      181 cttcggcaat ctccaaagcg tgcaggggga gacacctgga cggcagtcgc agcgaagcgg
      241 cgtcgtaccg cgttcctgca ggcggcgtgc agcaagcacg cagaactgta gcgcacggag
      301 gagaagtaca tgattaccaa ggccctgttg tgcgggtgcg tgtttggatg cctcgcggcc
      361 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnncgc ccgcgaacgc ctcgaccggc cgcttctttg cgatgctggc
      481 gacgcactga accaagccct gcgaccgccg ctgcggtcgc gactgacgaa atggccctgc
      541 ctccggactc tgtaggcagg gccgttttgc tttgcggcgc tcagtccttt gcgggcagct
      601 gggcgagggt atgcgccatg cggatgatgt agtggcggat tgcgtcggca tccgctgtcg
      661 tgagcgtctg cgcgaagccg ggcatgccgc gcgcggcgag caggccgtcg agcagcggac
      721 ggcggaacag cgcggcctcg ctcagatacg gtgagtggcg caggtcgggc atgccgccgc
      781 cggccaccgc cccggcccca tggcactgca tgcagtagcg cgcgtaacgc gccgcgccgt
      841 gcgcgacctc ggcttcggtg ccggtgtcgg gcggcggcgc cacgcgcggc cgcgcgctcg
      901 gctccggcat cgcggcctgg ccgcccagct tccacacgaa cagcccgccc ggcaccggcg
      961 cgcccgtgcg cggcagcagg gcgctcgagg tgatgtgcat cgagctaccg aagccggccg
     1021 cggcggcgat gtactgctcg ccgtcgagcg tgtagctgat cggcccaccg aggatgtcga
     1081 gccaggtgtc ggactgccac aggcggcggc cgtcgcgcgc atcgaaggcg gccaggccct
     1141 cgccgccgac gccctggaac accaggccgc cggcagtcgc cagcacgccg ccattccacg
     1201 agccggcgtg tggggtgcgc caggcggcgg tgcgacggac cgggtcccag gccagcaggt
     1261 ggccgcgcat cgaggccttg ccctgctcga ggaccgcgcg atcgggcggc atcggcggat
     1321 tgagcacgcc ggtattccag cgctcgggct cgtggcggaa cgccggctcc ttcatcatga
     1381 agccggcgtt ctcctgcgcc gggacataga ccaggccggt ctccgggctc caggccatcg
     1441 gttgccagtt gtggccgccg agctgcgagg gctggacgag gaagggctgg cccgcggggt
     1501 agcgcgcgcc tgcggtttcc accggacggc cgctggcggg gtcgacatgg ctcgcccaat
     1561 tcaccgccac gtacttgtcg gccgagagca gttcgccggt ggcgcgatcg aggacgtaga
     1621 agaagccgtt cttgggggcc tgcatcagta ccttgcgcgg ctggccgtcc accgccaggt
     1681 cggcgaggat catgtgctgg gtggcggtgt agtcccagct gtcgcccggg gtggtctggt
     1741 aatgccagac gtattccccg gaggcgcgcc gcaccgcgac gatcgaggac aggaacaggt
     1801 tgtcgccgcc gtccgggctg cgcaggtcgc ggtcgtgcgg cgagccattg ccgacgccga
     1861 agtagagcag gtcgagctcg gggtcccagg ccatcgaatc ccacaccgtg ccgccaccgc
     1921 cctgacgcca ccattcgccg gtccaggtcg gccgcgcgag cgtctccagc gggcggtccg
     1981 aggctgcccc gtccgcgccg tccgccgggt tgcccggcac ggtgtaaaag cgccagcgca
     2041 gcgcgcccgt ccgcgcgtcg taggccgaga cgaagccgcg cacgccgtac tcggcgccgc
     2101 cgttgccgat caccaccagg ccgcgcgcca cgcgcggtgc gccggtgatg gtgtagggcg
     2161 cggcgatgtc gaaggtcccg gtcgaccaca gcaggcggcc gtcccgggcg tccagcgcct
     2221 gcaggcgacc gtcgatggtg gcgacgaaga cgcggccgtc ctcgaccgcc acgccgcgat
     2281 tgaccacgtc gcagcaggcg tgcatgccct tgccggccgg cacctcgggg tcatgctgcc
     2341 acttgagttg gccgctgcga gcgtcgagcg catacaccac gccccacggt ccggtgacga
     2401 acagcgtgcc gtcgaccatg atcggcgtcg cctcgacccc gcgcgggcgg tcgaagcgga
     2461 agtgccaggc caggcccagg cgctcgacgt tggcggtatc gatcgccgac agcgggctga
     2521 agcgggtttc cgcggcgtcg aggccgtgcc gtggccattc gccggcgacg gccgcggccg
     2581 gcgaggaggg cgcggcggcg atcgccacag ccggggcggc gagggtgctg gccgccgcga
     2641 gcgcggcgag catcgttgtt ggccggaagc ggcgcatagc ggactccttc gggcacgacg
     2701 gcccgcgtgg tgggaatgac ctgcgcatca tcggccgagg gcgcgcgcgc cgaatcgtcc
     2761 gtccggaggg tctcctccgc ttcgcctagt cgatttgaac gattaccggc tgcagtgcct
     2821 gtggcatctt tgagcgcact acagggcggg cgatgcctgc atcagcgagg gaagggagca
     2881 tgggtctcaa aggaaacgca gcaatcgtcg gcgccgcgca gtacaa
//
LOCUS       DAOMPG010000276         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13924_length_2896_cov_3.320310, whole genome shotgun sequence.
ACCESSION   DAOMPG010000276 DAOMPG010000000
VERSION     DAOMPG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13924_length_2896_cov_3.320310"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..924
                     /gene="phoB"
                     /locus_tag="PLS34_11800"
     CDS             226..924
                     /gene="phoB"
                     /locus_tag="PLS34_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRP88184.1"
                     /translation="MTAKQILIVEDEKPIRDMIGFGLRRAGFEVREAEDCQGARVEVA
                     NRLPDLVLVDWMLPDMSGLELTRLLKKDPATREVPVIMLTARAEEEDKIRGLEGGADD
                     YVTKPFSPRELLARIKAVLRRGAPDEDEEVLVAGGLKLNSASHRVTAGEETVGLGPTE
                     YRLLKFFMSHPDRVYSRGQLLDRVWGGNVYVEERTVDVHIRRLRKALEPSGCDRLIQT
                     VRGAGYRFSEQVDV"
     gene            921..2258
                     /gene="phoR"
                     /locus_tag="PLS34_11805"
     CDS             921..2258
                     /gene="phoR"
                     /locus_tag="PLS34_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HRP88185.1"
                     /translation="MNPGPWLYVAARFAALLAAAVLLGYLFGHPFAWLSAVLLGYLGW
                     HLWHLWQLESWLRRKLGEPPRDAPGLWGGVFAQLHRLRLQSRARKKRLARVLKEFRKS
                     TQALPDAGVVLDKDHAIAWLNAAAERLLGLRPEDRGQRIDNLLRAPEFGEFLRKAGPG
                     QVLRLASPVDENIKLSLQLVPYGETQRLLLAKDITHEMRLETVRRTFVANASHELRSP
                     LTVISGYLDSLAEDPELAEHWREPVGEMARQSERMRRIIDDLLTLSRLEASAPDAERE
                     RVDVAGMLTVLRKEAIARPERPAAVELQLETDARLLGVESEIYSAFANVIGNALKYTP
                     ADGRVTIRWYLDGGQACLAVADTGVGIPAEAIPRLTERFYRVDKSRDRATGGTGLGLA
                     IVKHVLQRHGARLEVQSKLGEGSTFTCRFPADRVTREAPPANETVLTSPGKGA"
     gene            complement(2233..2697)
                     /locus_tag="PLS34_11810"
     CDS             complement(2233..2697)
                     /locus_tag="PLS34_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRP88186.1"
                     /translation="MGTEIERKFLVSGDGWRDGTPGRRIRQGYLSVDPARAVRVRTAD
                     ERAWLTVKGALTGLSRQEFDYAIPFADARQMLDTLCHQPLIDKTRYKIRHGEHLWDVD
                     EFHGANDGLVIAEVELDSEAESFDRPPWLGEEVSDDPRYFNMNLFRHPYRDW"
BASE COUNT          447 a         1004 c         1009 g          436 t
ORIGIN      
        1 ggcggcggcg gccctggcgc gcggcgaggc ggtggccgcc gaggacgcgc tgccggtgta
       61 cctgcgcgag caagtcgcct ggcgctgaat tggggacatt ccccaattcc ccggaattgg
      121 ggaatgtccc caattcagcg ccacaatcag taacatggca gcaggtttaa cgaatctgta
      181 acgcagcatt gctgtaatgc ccccgtcaca gacacgggcc gagccatgac cgccaagcag
      241 atattgatcg tcgaagacga aaagccgata cgggacatga tcggcttcgg gttgcggcgc
      301 gccggcttcg aggtgcgcga ggccgaggac tgccagggcg cgcgggtcga ggtggccaac
      361 cggctgccgg acctggtgct ggtggactgg atgctgcccg acatgagcgg gctcgagctg
      421 acccggctgc tgaagaagga tcccgccacg cgcgaggtgc cggtgatcat gctcaccgcc
      481 cgcgccgagg aggaggacaa gatccgcggc ctcgagggcg gcgccgacga ctacgtcacc
      541 aagcccttct cgccgcgcga actgctggcg cgcatcaagg cggtgctgcg ccggggcgcg
      601 cccgacgagg acgaagaggt gctggtcgcc ggcggcctga agctcaacag cgccagccac
      661 cgcgtcaccg ccggcgagga gaccgtcggg ctcggcccga ccgagtaccg gctgctgaag
      721 tttttcatga gccacccgga ccgcgtctac agccgcggcc agctgctcga ccgcgtctgg
      781 ggcggcaacg tgtacgtcga ggaacgcacc gtcgacgtgc acatccggcg gctgcgcaag
      841 gcgctcgagc cctccggctg cgaccggctg atccagacgg tgcgcggcgc cggctaccgc
      901 ttctccgagc aggtcgacgt gtgaaccccg ggccgtggct gtacgtcgcg gcgcgcttcg
      961 ccgcgctgct cgccgccgcg gtcctgctcg gctacctgtt cggccacccc ttcgcctggc
     1021 tgtcggcggt gttgctcggt tatctcggct ggcacctgtg gcacctatgg cagctggagt
     1081 cctggctgcg ccgcaagctc ggcgagccgc cgcgcgacgc gccggggctg tggggcggcg
     1141 tgttcgccca gttgcaccgc ctgcgcctgc agagccgcgc gcgcaagaag cgcctggcgc
     1201 gcgtgctcaa ggagttccgc aagtccaccc aggcgctgcc cgacgccggc gtggtgctcg
     1261 acaaggacca cgccatcgcc tggctgaacg ccgcggccga gcgcctgctc ggactgcggc
     1321 cggaagatcg cggccagcgc atcgacaacc tgctgcgcgc gccggagttc ggcgagttcc
     1381 tgcgcaaggc cggccccgga caggtgctgc ggctggcctc gccggtggac gagaacatca
     1441 agctgtcgct gcagctggtg ccctacggcg agacgcagcg gctgctgctg gccaaggaca
     1501 tcacccacga gatgcgcctc gagaccgtgc ggcgcacctt cgtggcgaac gcctcgcacg
     1561 agctgcgctc gccgctgacg gtgatcagcg gctatctcga ctcgctcgcc gaggacccgg
     1621 agctggccga gcactggcgc gagccggtcg gcgagatggc gcgccagtcg gagcgcatgc
     1681 ggcgcatcat cgacgatttg ctgacactgt cgcggctgga ggcctcggcg cccgatgccg
     1741 agcgcgagcg ggtggacgtc gccggcatgc tcacggtgct gcgcaaggaa gcgatcgcgc
     1801 ggccggaacg gccggccgcg gtggagctgc agctggagac cgatgcgcgg ctgctcgggg
     1861 tcgagtcgga aatctactcg gccttcgcca acgtcatcgg caacgcgctg aagtacacgc
     1921 ccgccgacgg ccgcgtgacc atccgctggt acctcgacgg cggccaggcc tgcctggcgg
     1981 ttgccgacac cggcgtcggc atccccgcgg aggccatccc acgccttacg gagcgcttct
     2041 accgcgtgga caagagtcgc gaccgggcca ccggcggcac cgggctcggc ctggccatcg
     2101 tcaagcacgt cctgcagcgc cacggcgcgc gcctggaggt gcagagcaag ctgggcgagg
     2161 gcagcacctt cacctgccgc tttccggcgg accgggtgac gcgcgaggcg ccgccggcca
     2221 acgaaaccgt gctcaccagt cccggtaagg gtgcctgaac aggttcatat tgaaatagcg
     2281 cgggtcgtcc gagacctctt cgccaagcca cggtggccga tcgaagctct ccgcctcgct
     2341 gtcgagttcg acttccgcga tcacgaggcc gtcattcgca ccatgaaatt cgtccacgtc
     2401 ccagagatgt tcgccgtgcc ggatcttgta gcgcgtcttg tcgatcaacg gctggtgaca
     2461 gagcgtatcc agcatctgcc gcgcgtcggc gaacgggatc gcgtagtcga actcctggcg
     2521 gctcagcccg gtcagcgcgc ccttgaccgt cagccaggcg cgttcgtcgg cggtacgcac
     2581 gcggacggct cgggccgggt ccaccgacag gtagccctgg cggatgcggc ggccgggtgt
     2641 accgtcccgc cagccgtcgc cgctcaccag gaacttgcgt tcgatttcgg tgcccatcgg
     2701 cgcatctcat cactcgcccg gaaagcctgt caaacaggcg gcgccagcgc gggcgacttc
     2761 gactccataa caggtgaatg ccatgtccaa gctgcatgac gcgatcttcc tgctggtgct
     2821 ttcgctggcg ctgggtgcct gcgcggcgcc caccatcccg ttgcacgatc cggcgagtga
     2881 cgcgggtctg ccggcc
//
LOCUS       DAOMPG010000277         2894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13935_length_2894_cov_4.551250, whole genome shotgun sequence.
ACCESSION   DAOMPG010000277 DAOMPG010000000
VERSION     DAOMPG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13935_length_2894_cov_4.551250"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLS34_11815"
     CDS             complement(<1..263)
                     /locus_tag="PLS34_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRP88187.1"
                     /translation="MRRRAPYGSWESPLDAAEVARAATRIFQPRRNGDQVNWLELRPA
                     EGGRMVLMQDLRGQRTELTPPPYSARSRVHEYGGGSYCVAGRD"
     gene            complement(263..1030)
                     /locus_tag="PLS34_11820"
     CDS             complement(263..1030)
                     /locus_tag="PLS34_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRP88188.1"
                     /translation="MCLLVFAWRPDAAERLLFAGNRDEFHDRPAAPADWWENGLLGGR
                     DLRAGGTWLATHRSGRFAVVTNFREPLEEGRGPRSRGELVTGFLDDTVTPAAFARDVA
                     ARGHEYAGFNLLLGDPESLVYLSNRGRGPEPLAAGVYGLSNHLLDTPWPKLERTRARF
                     RKLLEQGADTDALLDMLADRTPAADDLLPDTGVGPEFERLLSSPFIQSPRYGTRCSTV
                     MRMLEDKQMEFVERRFDANGTITGQSRFRFPIDGVEE"
     gene            complement(1051..1983)
                     /gene="nudC"
                     /locus_tag="PLS34_11825"
     CDS             complement(1051..1983)
                     /gene="nudC"
                     /locus_tag="PLS34_11825"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRP88189.1"
                     /translation="MSRPRSHLLAATSLDRLGHLRKDAAWLDAARRDGRYVAVWRRKV
                     LVSAGERPAPLLLAADMLAALDASRDSVLLGERDGRPCFAVALATEAPSSAGRFEDVR
                     GLGDRLAPDDAALLAYARAMILWHERHGHCGVCGAPTESIEAGHARECRACGAKHFPR
                     VDPAIIVLVADAQRCLLGRQAAWPAGRYSTIAGFVEPGESLEDAVCREVLEETGVRVE
                     EVAYHGSQPWPFPSSLMLGFHARPAGGEIALRDGELEDARWFDRDDIAAQRVLLPPRV
                     SIAYRLIEEWFDRAPGRRLAEETEPGPWIARPEA"
     gene            complement(1980..2471)
                     /gene="rraA"
                     /locus_tag="PLS34_11830"
     CDS             complement(1980..2471)
                     /gene="rraA"
                     /locus_tag="PLS34_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HRP88190.1"
                     /translation="MAFNTADLCDAHEADLHVVESMFHRYGRLVCFDGPIATVKCHED
                     NSKVREALEEPGDGRILVVDGGGSQRRALLGDMLGELAVKNGWKGIVINGAIRDSAAI
                     NRLELGVRALGTIPLKTHKRGDGQRDVSVSFAGVTFRPGEHLYADADGIVVAARSLDL
                     TGE"
     gene            complement(2529..>2894)
                     /locus_tag="PLS34_11835"
     CDS             complement(2529..>2894)
                     /locus_tag="PLS34_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88191.1"
                     /translation="RNPWVQAEASLLAHVFAAWGLLRIRPDLSTAPALPADRLEFGRR
                     FCDAYEEFCATHPATAISFEHAWGLLHSLLAGRDELLLLDCGACNAFYVQDALALDGR
                     RCPACRVAPRRRERPRDQA"
BASE COUNT          421 a         1040 c         1036 g          397 t
ORIGIN      
        1 cagtcacggc cggccacgca gtagctgccg ccaccgtact cgtggacccg gctgcgggcg
       61 ctgtatggcg gtggcgtgag ctccgtgcgc tgcccgcgca aatcctgcat gagcaccatc
      121 cggccgccct ccgccggccg caactccagc caattgacct ggtcgccgtt gcggcgcggc
      181 tggaagatgc gggtcgcggc ccgcgcgacc tccgcggcgt cgagcggcga ttcccagctg
      241 ccgtagggcg cgcgccgccg catcactctt ccaccccgtc gatggggaac cggaagcgcg
      301 actggccggt gatcgtgccg ttcgcatcga aacggcgttc gacgaattcc atttgcttgt
      361 cctccagcat tcgcatgacg gtcgagcagc gcgtcccgta gcgcgggctc tggatgaacg
      421 gggacgacag cagccgctcg aactcggggc cgacgccggt gtccggcagc aggtcgtccg
      481 ccgccggcgt gcggtccgcc agcatgtcca gcagcgcgtc ggtgtcggcg ccctgctcca
      541 gcagcttgcg aaaacgcgca cgagtgcgct cgagtttcgg ccacggcgtg tcgagcaggt
      601 gattcgacag cccgtagacg ccggcggcca gcggttccgg gccgcgtccg cgattggaaa
      661 ggtagaccag gctctcggga tcgcccagca gcaggttgaa tccggcgtat tcgtggccgc
      721 gcgcggccac gtcgcgggca aaggccgccg gtgtcacggt gtcgtcgagg aacccggtga
      781 ccagttcgcc gcgcgagcgc ggtccccggc cttcctcgag cggctcgcga aagttggtca
      841 cgaccgcaaa gcggccgctg cgatgggtgg ccagccaggt gccgccggcg cgcaggtcgc
      901 gcccgccgag cagaccgttc tcccaccagt cggccggtgc cgccggacga tcgtggaact
      961 cgtcgcgatt gcccgcgaac agcagccttt cggcggcgtc gggacgccag gcgaacacca
     1021 gcaggcacat cgctcaggtc tccgttgcca tcaggcctcg ggcctcgcga tccagggccc
     1081 gggctcggtt tcctcggcca gccggcggcc cggcgcccgg tcgaaccact cctcgatcag
     1141 gcggtaggcg atcgatacgc gcggcggcag cagcacgcgc tgcgcggcga tgtcgtcacg
     1201 atcgaaccag cgcgcatctt ccagctcgcc gtcgcgcagc gcgatctcac cacccgccgg
     1261 ccgggcgtgg aagcccagca tcagggaaga aggaaacggc cacggttgcg agccgtggta
     1321 ggccacttcc tccacgcgca cgccggtctc ctccagcact tcgcggcaca ccgcgtcctc
     1381 gaggctttcg cccggctcca cgaagccggc gatggtcgaa tagcggcccg ccggccaggc
     1441 ggcctgccgt cccagcaggc agcgctgggc gtcggcgacc agcacgatga tcgccgggtc
     1501 gacgcgcggg aagtgcttcg cgccacaggc cctgcattcg cgcgcgtggc cggcctcgat
     1561 gctctcggtc ggggcgccgc agacgccgca gtggccgtgc cgttcgtgcc acaggatcat
     1621 ggcgcgcgca taggcgagca gcgcggcgtc gtcgggcgcc aggcggtcgc cgagcccgcg
     1681 gacgtcctcg aaacggccgg cagacgaagg cgcctcggtg gcgagcgcga ccgcgaaaca
     1741 gggccggccg tcgcgctcac ccagcagcac gctgtcgcgc gacgcatcga gcgcggcgag
     1801 catgtcggcg gcgagcagca gtggcgcagg acgctcgccg gcgctgacca gaaccttgcg
     1861 tcgccatacg gcgacatagc gtccgtcccg gcgcgccgcg tcgagccagg cggcgtcctt
     1921 gcgcaggtgg cccagccggt ccagcgaagt ggcggcgagc aagtggctcc gggggcggct
     1981 cattccccgg tgagatccag cgaccgcgcc gcgaccacga tgccgtcggc gtccgcatac
     2041 aggtgctcgc cgggacggaa ggtgacgccg gcgaaactga ccgagacgtc ccgctggccg
     2101 tcgccccgct tgtgggtctt gagcgggatg gtgccgagcg cgcgcacgcc cagctcgagc
     2161 cggttgatgg cggccgaatc gcggatcgcg ccgttgatga cgatgccctt ccagccgttc
     2221 ttcaccgcca gttcgccgag catgtcgccg agcagggcgc ggcgctgcga gccgccgccg
     2281 tccaccacca ggatgcggcc gtcgcccggt tcttcgaggg cctcgcgcac cttggagttg
     2341 tcctcgtggc atttcaccgt ggcgatcggg ccgtcgaagc acaccagccg gccgtagcga
     2401 tggaacatcg actcgacgac gtgcaggtcc gcttcgtggg cgtcgcaaag gtccgcggtg
     2461 ttgaaggcca tggtcgctcc cgctccgggt ttcgcgccat attctacgct gcgccggcaa
     2521 caggcgggtc aggcctggtc gcgcggccgc tcgcgccggc gcggcgccac ccggcaggcc
     2581 ggacagcgcc gcccgtcgag ggcgagcgca tcctgcacat agaaggcgtt gcaggcgccg
     2641 cagtcgagca gcagcagctc gtcccggccc gccaggaggg agtgcagcag gccccaggcg
     2701 tgctcgaagc tgatggcggt cgcaggatgc gtcgcgcaga actcctcgta ggcatcgcag
     2761 aagcgccggc cgaactcgag gcggtcggcc ggcaaggccg gtgccgtgct caggtcgggc
     2821 cggatgcgca gcagccccca ggcagcgaac acgtgggcga gcaggctcgc ctcggcctgc
     2881 acccacgggt tgcg
//
LOCUS       DAOMPG010000278         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_13969_length_2888_cov_3.483939, whole genome shotgun sequence.
ACCESSION   DAOMPG010000278 DAOMPG010000000
VERSION     DAOMPG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13969_length_2888_cov_3.483939"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PLS34_11840"
     CDS             complement(<1..1169)
                     /locus_tag="PLS34_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRP88192.1"
                     /translation="MQQTRFLLSAGLAGLAAAAPLTVAADAAPTLDLGEVVVTAQGPR
                     TVELVGSVDEISADDIRRSGARNLEEAIDLLPGLYVRYGGDGTPRIDIRGLRTRNVLL
                     FLDGVPLNSSLDGQFDAAAIPVESIARIKVTRGGSSVLYGAGGNAGVINIITRGADVD
                     GTDASLRAERGFQRSGQVRGHVASRAGKFGVMASGSWFDRDYFELSDDFVPTSLEDGG
                     RRENSDRKDKAMTVNLGWDPTASVRGGLTVSHREGELGKPPITISSADSDYANRTRYE
                     RAEYADVNVHTALSWQISEQLSLRPTLFFNRRNEQTDSYDDAGYANQAAAGSFSEDAT
                     TKITGGGLQLAHRISDAGLLTFGSNGRKEEWQASGFTMTAPGGGGGGGGGGGGGGG"
     assembly_gap    1555..1564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1565..2295
                     /locus_tag="PLS34_11845"
     CDS             <1565..2295
                     /locus_tag="PLS34_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP88193.1"
                     /translation="LDHLLPQFEAKSGYRVEVYSGSDVYDRARRGEADLVISHLGKGA
                     VEDFVTSGAGLWPRPVFASQMVIIGPASDPAGIRSTQDPIEAMRRIAAAKAGFVVGES
                     VGRRYLAALLLAGAGAPDRGDWFEQKDFSGNELIRYAEERGAYALVKGPAFAQFSVRH
                     ESGMRILLDESPLLYRVMAIVPVNPARVDGINTAGVAALVEYLLSAETQAAIANFRHP
                     ATGVPMWSPAARHNHSKGLPRPRG"
     gene            complement(2314..2523)
                     /locus_tag="PLS34_11850"
     CDS             complement(2314..2523)
                     /locus_tag="PLS34_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRP88194.1"
                     /translation="MKISARNVLRGTVKDVKSGPVNSQVTVDIGGGNSITSMITSDAV
                     KDLGLASGKPVYVVIKASEVILGTD"
     gene            complement(2593..>2888)
                     /locus_tag="PLS34_11855"
     CDS             complement(2593..>2888)
                     /locus_tag="PLS34_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRP88195.1"
                     /translation="MIASGAADAGFGVEAAAARFGLDFVPLVRERYLLAMERSSPPPS
                     WLETLIGALQGDEFRTRMRSIPGYDLADSGQVVELAGAPRPDAGTRAAGADYA"
BASE COUNT          448 a          987 c          962 g          481 t
ORIGIN      
        1 ccgccgccac cgccaccgcc accgccaccg ccaccgccac cgccgggcgc ggtcatggtg
       61 aagccgctgg cctgccattc ttccttgcgc ccgttggagc cgaaggtcag caggccggcg
      121 tccgagatgc gatgcgccag ctgcaggccg ccgccggtga tcttggtggt cgcatcttcg
      181 ctgaaggagc cggccgcggc ctggttcgcg tagccggcgt cgtcgtagct gtcggtctgc
      241 tcgttgcgcc ggttgaagaa cagggtcgga cgcaggctga gttgctcgga aatctgccag
      301 gacaacgccg tgtgaacgtt gacgtcggcg tactcggccc gctcatagcg tgtccggttg
      361 gcatagtcgg agtcggcact gctgatcgtg atcggcggtt tgcccaattc gccttcgcgg
      421 tgactcaccg tcaggccgcc ccgcacactg gccgtcggat cccagccgag attgacggtc
      481 atggccttgt ccttgcggtc gctgttttcg cggcggccgc cgtcctccag tgaagtcggg
      541 acgaaatcgt ccgacaactc gaaataatcg cgatcgaacc aggagcccga ggccatcacc
      601 ccgaacttcc cggcgcggga cgccacatgg ccgcgcacct ggccggagcg ctggaagccg
      661 cgctcggcgc gcaggctcgc atccgtgccg tcgacgtcgg cgccgcgggt gatgatgttg
      721 attacgccgg cattcccgcc ggcaccatag aggaccgacg agccgccgcg cgtcaccttg
      781 atgcgtgcaa tgctctcgac cgggatggcg gcggcgtcga actggccgtc gagcgaggag
      841 ttcagcggca cgccgtcgag aaacagcagc acgttcctgg tcctgagccc gcggatatcg
      901 atgcgcggcg tgccgtcgcc accgtagcgc acgtacaggc cgggcagcag gtcaatcgcc
      961 tcctccagat tgcgtgcgcc ggagcggcgg atgtcgtccg ccgagatctc gtctaccgac
     1021 ccgaccagct cgaccgtccg cggaccttgt gcggtaacca cgacctctcc gaggtcgaga
     1081 gtgggtgcag catcggcggc gaccgtcagc ggggcggccg ccgcgaggcc ggccaggccg
     1141 gcactcagca ggaatctggt ttgttgcatg ttattgccac tctattgcgc atcaggggaa
     1201 acggagcggg cagtatgcca gagtctggcg gatctcaatt ggcacgaagt tgcataactt
     1261 ggcccctcgc gcacagcgcg cacgccagag tttttgtggc cgcagcaaaa gtgttaccgt
     1321 tccggcccac gcgctgccgg cggccctcgc ggaccgccgg cagcggccaa tatccaggat
     1381 cgtccccgga ggcccggcat gaagactcag caccagaacg ccatcaccca cgtcgtcggc
     1441 ctgctgttgg ccgcggtact gttcacggcc ggtgacgcgc cggcgcatgc ggaccaagcc
     1501 gatgcgaaaa aaaccgtgcg gctcgcggtg gtcaagacgc cggcgcagac gggcnnnnnn
     1561 nnnntgctcg accacttgct gccgcagttc gaagcgaaat caggctaccg ggtggaggtc
     1621 tacagcggca gcgacgtcta tgacagggcc cgcaggggcg aggccgacct ggtcatttcg
     1681 cacctgggca aaggcgccgt cgaagatttc gtgacctccg gcgccggcct ctggccgcga
     1741 ccggtgttcg ccagccagat ggtcatcatc ggcccggcaa gcgaccctgc gggcatccgc
     1801 agcacgcagg acccgatcga ggccatgcgg cgcatcgcgg ccgccaaggc gggcttcgtg
     1861 gtgggtgaga gcgtcggtcg ccgttacctg gcggccctgc tgctcgcggg cgccggcgcg
     1921 ccggaccgcg gcgactggtt cgagcagaag gacttctcgg gcaatgaact cattcgctac
     1981 gccgaagagc gcggcgctta cgcactcgtc aaggggcccg ctttcgcgca gttcagcgtg
     2041 cggcacgagt ccggcatgcg gatcctgctc gacgagtcgc cgctgctgta tcgcgtcatg
     2101 gcgatcgtgc ccgtcaaccc tgccagggtg gacggcatca acacggccgg cgtcgcggcc
     2161 ctggtcgagt acctgttgtc agccgaaacc caggcggcga tcgccaactt caggcacccg
     2221 gcgaccggcg tcccgatgtg gtcgccagcg gcgcgccaca atcattccaa gggtctgccg
     2281 cgaccgcgcg ggtgacgcgg gccggccggc ctctcagtcg gtgccgagga tgacctcgga
     2341 ggccttgatc accacataga ccggcttgcc cgaggcgagg ccgaggtcct tgacggcatc
     2401 gctggtaatc atcgaggtga tgctgttgcc gccgccgatg tccaccgtga cctggctgtt
     2461 gacgggtccg gacttcacat ccttgacggt gcccctgagt acgttccgtg cgctgatctt
     2521 catcgaggta tctccatctg ctgactattt cgtggcgggg cattccggcc ctatggcctg
     2581 ctctcggcca cctcaagcat agtctgctcc cgccgcgcgt gtccccgcat cgggccgggg
     2641 ggcgccggcg agctccacca cctggccgct gtcggcaagg tcgtagccgg ggatgctgcg
     2701 catcctggtc ctgaactcgt cgccttgcag cgccccgatc agcgtttcga gccaggatgg
     2761 cggcgggctg ctgcgctcca tggccagcag gtagcgttcc ctcaccagcg gcacgaaatc
     2821 cagtccgaac cgcgcagcgg ccgcttcgac cccgaagccg gcgtccgccg cgccgctggc
     2881 gatcatcg
//
LOCUS       DAOMPG010000279         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14056_length_2874_cov_3.023413, whole genome shotgun sequence.
ACCESSION   DAOMPG010000279 DAOMPG010000000
VERSION     DAOMPG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_2874_cov_3.023413"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLS34_11860"
     CDS             complement(<1..148)
                     /locus_tag="PLS34_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HRP88196.1"
                     /translation="MIVLDTHVLLWWLNGDPAVSPRARSAIRREQGDENGLIMVSAIS
                     AWEIA"
     gene            complement(145..411)
                     /locus_tag="PLS34_11865"
     CDS             complement(145..411)
                     /locus_tag="PLS34_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRP88197.1"
                     /translation="MTSPHDYGDAVHRKHVSKSEFKARALEYFRAVEASGDAVIVTDN
                     GQPRIEIRRYRADERSPLERLRGSIVEFKDPTLPVAEDDWEAGA"
     gene            797..1696
                     /locus_tag="PLS34_11870"
     CDS             797..1696
                     /locus_tag="PLS34_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88198.1"
                     /translation="MITVVRVTETRPRAVLEVLRGIRRRHPEWPLIVAQTCLHTAYPD
                     GAEHVLPFPFNSPDWQARVPADLRRLLLAQRKAFAELPGAGGVLWVPVDFTLPEDGFA
                     PADYGADALWDAIEHAMFMDLRARVIADEEVADVFGRAAHPKVVGYSLAAAVAGGVPV
                     ADLALLPSVQAAMIYALAETYGVQWTARNTSEFLGLLGAGFLVAYGGRSLGRSLVKLV
                     PVWGQTAGIAWGAAAGGGITFGLGKAACHYLARKRAGRTVSAEQLRGVYRDALAEGRA
                     LVREQLAASSRAAGARDDEGAGR"
     gene            1693..>2874
                     /locus_tag="PLS34_11875"
     CDS             1693..>2874
                     /locus_tag="PLS34_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRP88199.1"
                     /translation="MKAVLELFGRLRLVAAGLALLPLVGLPVLGVIWLVQSSAGLWWL
                     AAGLVALAFAAWFHWLAGRREAAALAEVLAGARTAPGGNWPRQADECWVKVQQMADEA
                     TPTAWPLDEPAGLGRLAEEVLRLVAGHFYPRSSQPLAEMTVPDTLMVIERAARELRLE
                     VTENVPGSHRITLAQVAGARRLKGHYDRYSPLIKVLQAAVMPQSLLRQLLGDMLLGSV
                     FQAGSKNVQRWLLQEYIRKIGFHAIELYGGLVVLEDGRALEAATRDSTTDLARAGAAE
                     AARLEPLRIVLLGRVNAGKSSLVNALFGELVAATDVLPDSTTGIVPYRLERDDELRAL
                     VYDTPGFDADGVDAKGLEQAGLAADLVIWVTAADRPDRAEERARLDRLRQGLADPARR
                     AA"
BASE COUNT          375 a          924 c         1152 g          423 t
ORIGIN      
        1 tggcgatttc ccatgccgag atggcggata ccattatcag tccgttctcg tcgccttgct
       61 cacgccggat agcactcctc gcgcggggtg acacggcggg gtccccgttg agccaccaga
      121 gcagaacgtg ggtatccagg acgatcaagc acccgcttcc cagtcatcct cggccaccgg
      181 cagcgtgggg tccttgaact ccacgatgga gccgcgcaaa cgctccagcg gcgagcgctc
      241 atccgcccgg taccgccgta tctcgatcct cggctgaccg ttgtcggtga cgatcacggc
      301 gtccccggag gcctcgacgg cgcggaaata ttccagggcc ctggccttga actccgattt
      361 tgagacgtgt ttccggtgca ctgcgtcacc atagtcatgg gggctagtca tgacaatagc
      421 gggcaccggt gaccagggca agtggtacaa actagccaaa tgggcgcatt aaacaagctc
      481 aagcaggcgc tcggcgcggc ggcgaccgac ccgggccggg tcgcggcggc gttggaggcg
      541 gcggcgcaga agcagccgcc gccggtgctg tggctcttgg gcgcgaccca ggccggcaag
      601 acctcgatca tccgcacgct gaccggcaac acggcggcgg agatcggcaa cggctttcgg
      661 ccgtgcacgc ggcgctcgta cctgtacgag ttcccggcgg gcgcgccggt gctcggcttc
      721 ctcgacacgc gcgggctcgg cgaggtggcc tacgacccgg cggaggacat cgcgctatgc
      781 gacgcgcgcg cacctggtga tcacggtggt gcgggtgacg gagacgcggc cgcgggcggt
      841 gctcgaggtg ctgcgcggca tccgcaggcg gcacccggag tggccgctca tcgtcgcgca
      901 gacctgcctg cataccgcct atccggacgg agcggagcac gtgctgcctt ttcccttcaa
      961 ctcgccggac tggcaggcgc gcgtgcctgc cgacctgcgg cggttgcttc tggcgcagcg
     1021 caaggcgttt gccgagctgc cgggcgcggg cggggtgctg tgggtgccgg tggacttcac
     1081 gctgcccgag gacggcttcg cgccggccga ctacggcgcg gacgcgctgt gggacgcgat
     1141 cgagcacgcg atgttcatgg acctgcgcgc gcgcgtgatc gccgacgagg aggtggcgga
     1201 cgtattcggg cgggcggcgc acccgaaggt ggtgggctac agcctggccg ccgcggtggc
     1261 gggcggggtg ccggtggcgg acctggcgct gttgccgagc gtgcaggcgg ccatgatcta
     1321 tgcgctggcc gagacctacg gcgtgcagtg gacggcgcgc aacaccagcg agttcctcgg
     1381 gctgctgggc gccgggttcc tcgtcgccta cggcgggcgg tcgctcggcc gcagcctggt
     1441 caagctggtg ccggtgtggg gccagacggc cggcatcgcg tggggcgcgg cggccggcgg
     1501 cggcatcacc ttcgggctgg gcaaggcggc gtgccattac ctggcgcgca agcgcgcggg
     1561 aaggacggta tcagcggagc agctgcgcgg cgtatatcgg gacgcgctgg cggagggccg
     1621 ggcgctggtg cgcgagcagc tcgcggcaag cagccgggcg gccggcgcgc gcgatgacga
     1681 gggcgccggc cgatgaaggc cgtgctcgag ctcttcggtc ggctgcggct ggtggccgcg
     1741 gggctggcgt tgttgccgct ggtcggcttg ccggtgctcg gtgtgatctg gctggtgcag
     1801 tcgtcggccg gcctgtggtg gctggcggcc gggctggtgg cgctggcctt cgcagcgtgg
     1861 ttccactggt tggccgggcg gcgcgaggcg gcggcgctgg cggaggtgtt ggccggggcc
     1921 aggacggcgc cgggcggcaa ctggccgcgg caggcggacg agtgctgggt gaaggtgcag
     1981 cagatggcgg acgaggcgac gccgacggcg tggccgctcg atgagccggc cgggctgggg
     2041 cgcctcgccg aggaggtgct gcggctggtg gcggggcact tctatccgcg gtcctcgcag
     2101 ccgctggccg agatgaccgt gccggacacg ctgatggtga tcgagcgcgc ggcgcgcgag
     2161 ctgcggctgg aagtcacgga gaacgtgccg ggcagccatc gcatcacgct cgcgcaggtg
     2221 gccggggcgc gccggctgaa gggccactac gaccgctact cgccgctcat caaggtgctg
     2281 caggcggcgg tgatgccgca gtccctgctg cgccagctgc tcggcgatat gctgctgggc
     2341 tctgtgttcc aggcgggctc gaagaacgtg cagcgctggc tgctgcagga gtacatccgc
     2401 aagatcggct ttcacgccat cgagctgtac ggcgggctgg tggtgctcga ggacgggcgt
     2461 gcgctggagg cggccacgcg cgactcgacg acggacctgg cgcgcgccgg ggcggcggag
     2521 gcggcgcgcc tcgagccgct caggatcgtg ctgctcgggc gcgtcaacgc cggcaagtcg
     2581 agcctggtca acgcgctgtt cggcgagctg gtggcggcca ccgacgtgct gccggactcg
     2641 accacgggca tcgtgccgta ccggctggag cgcgacgacg agctgcgcgc gctggtgtac
     2701 gacacgccgg gcttcgacgc ggacggcgtg gacgcgaagg gactggagca ggccggcctc
     2761 gcggcggacc tcgtcatctg ggtgacggcc gcggaccggc cggaccgggc cgaggagcgg
     2821 gcgcgcctcg accggctgcg gcagggtctt gccgacccgg cgcggcgcgc ggcg
//
LOCUS       DAOMPG010000280         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14371_length_2820_cov_4.014467, whole genome shotgun sequence.
ACCESSION   DAOMPG010000280 DAOMPG010000000
VERSION     DAOMPG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14371_length_2820_cov_4.014467"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLS34_11880"
     CDS             complement(<1..538)
                     /locus_tag="PLS34_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRP88200.1"
                     /translation="MSAADRRSARRPDPDKALVDIADYVEGYRIGSAAALETARYCLM
                     DSLACAFQALDYPACTRLLGPVVPGATLPGGARVPGTHWELDPVQAAFNIGALVRWLD
                     FNDTWLAAEWGHPSDNLGAILAVADYLSRQRAAEGGDPLRVRAVLEAMIVAHEIQGVL
                     ALENSFNRVGLDHVLLVRI"
     gene            complement(564..1688)
                     /gene="prpC"
                     /locus_tag="PLS34_11885"
     CDS             complement(564..1688)
                     /gene="prpC"
                     /locus_tag="PLS34_11885"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HRP88201.1"
                     /translation="MSKDKKAGGLAGVSAGETAICTVGKEGAGLTYRGYDIYDLAEHA
                     HFEEVAYLLHYGRLPTQAELDAYVKRLKGMRALPEALKNALEAIPGNSHPMDVLRSGC
                     SLLGNFEPEDDHQPQVDVADRLLAAFPAMLLYWWRFHQDGVRIDTVTDDDSVAGHFLH
                     LLHGKAPSEFHRRVLDVSLILYAEHEFNASTFTARVIAATLSDFHSAITGAIGALRGP
                     LHGGANEAAMELIERFDSAAAADRGVREMLAAKAKIMGFGHRVYTTSDPRNKVIKAFA
                     QQLSHECGDKVIFPVSEAIEKVMWDEKKLFPNADFYSASAYRFMGIPTALFTPIFVCS
                     RTTGWSAHVMEQRANNRLIRPGADYVGPGLQAWTPIRERG"
     gene            complement(1685..2599)
                     /gene="prpB"
                     /locus_tag="PLS34_11890"
     CDS             complement(1685..2599)
                     /gene="prpB"
                     /locus_tag="PLS34_11890"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HRP88202.1"
                     /translation="MSQAQSAGARFRAALEVERPLQVVGTINAYTALLAERAGYRAIY
                     LSGAGVANASFGLPDLGMTSLNDVCEDIRRITGATELPLLVDADTGWGAAFNIARTVQ
                     EMIRAGAGALHLEDQVSAKRCGHRPGKALVSSQEMCDRIRAAVDGRTDDAFVIMARTD
                     AHAVEGQAQAIERARAYVEAGADMIFAEALTTLDEYRQFTAAVSVPVLANITEFGKTP
                     LFTTRELGEAGVRLALYPLSAFRAMSAAALAVYETLRREGTQQAVLDTMQNRMELYEV
                     LGYHDYERKLDALFATDGAATKSQGDQQ"
BASE COUNT          402 a          964 c         1014 g          440 t
ORIGIN      
        1 cgatgcgcac cagcagcacg tggtcgagcc cgacgcggtt gaagctgttc tccagcgcaa
       61 gcacgccctg gatctcgtgc gccacgatca tcgcctcgag caccgcgcgg accctcaacg
      121 ggtccccgcc ctcggccgcg cgctggcgcg acaggtagtc ggccacggcg aggatggcgc
      181 cgaggttgtc cgacgggtgg ccccactccg ccgccagcca ggtgtcgttg aagtccagcc
      241 agcgcaccag ggcgccgatg ttgaaggccg cctgtaccgg gtcgagttcc caatgggtgc
      301 cgggcacgcg cgcgccgccc ggcagggtcg cgccgggaac caccgggccg agcagccggg
      361 tgcaggccgg gtaatccagc gcctggaagg cgcaggccag cgagtccatc aggcagtagc
      421 gcgcggtctc cagcgccgcg gcgctgccga tgcggtagcc ctccacgtag tcggcaatgt
      481 cgaccagcgc cttgtccgga tcggggcgcc gggccgagcg cctgtctgcg gcggacatgg
      541 gtcccgggcc tcctgcggct gcctcagccg cgctcgcgga tcggcgtcca ggcctgcagg
      601 ccggggccga cgtagtcggc gcccggccgg atgaggcggt tgttggcgcg ctgctccatg
      661 acgtgcgccg accagccggt ggtgcgcgag cagacgaaga tcggcgtgaa cagggccgtc
      721 gggatgccca tgaagcgata ggcgctggcc gagtagaagt cggcgttcgg gaacagcttc
      781 ttctcgtccc acatgacttt ctcgatggcc tcggacaccg ggaagatgac cttgtcgccg
      841 cactcgtgcg acagctgctg cgcgaacgcc ttgatgacct tgttgcgcgg gtccgaggtc
      901 gtgtacacgc ggtgaccgaa gcccatgatc ttggccttgg ccgccagcat ctcgcgcacg
      961 ccgcggtcgg ccgcggccgc gctgtcgaag cgctcgatca gctccatggc ggcctcgttg
     1021 gcgccgccgt gcagcgggcc gcgcagcgcg ccgatggcgc cggtgatcgc cgagtggaaa
     1081 tcggacagcg tggcggcgat gacgcgcgcc gtgaaggtcg aggcgttgaa ctcgtgctcg
     1141 gcgtacagga tcagcgacac gtccagcacg cggcggtgga actcggacgg cgccttgccg
     1201 tgcaggaggt gcaggaaatg tccggcgacg ctgtcgtcgt cggtcacggt gtcgatgcgc
     1261 acgccgtcct ggtggaagcg ccaccagtac aggagcatgg cgggaaacgc ggccagcaac
     1321 cggtcggcga cgtccacctg cggctggtgg tcgtcctccg gctcgaagtt gccgagcaac
     1381 gagcagccgc tgcgcagcac gtccatcggg tgcgagttgc ccgggatggc ctcgagcgcg
     1441 ttcttcagcg cctccggcag cgcgcgcatg cccttgaggc gcttgacgta ggcatcgagc
     1501 tccgcctgcg tcggcaggcg cccgtagtgc agcaggtagg cgacttcctc gaagtgagcg
     1561 tgctcggcga gatcgtaaat gtcgtagccg cgataggtca ggccggcgcc ttccttgccg
     1621 acggtgcaga tggccgtttc cccggcagac acgcccgcga gcccgcctgc tttcttgtcc
     1681 ttgctcattg ttggtctccc tggctcttcg tagcggcgcc gtccgttgcg aacagcgcgt
     1741 cgagcttgcg ctcgtaatcg tggtagccca gcacttcgta gagctccatg cggttctgca
     1801 tggtgtcgag gaccgcctgc tgcgtgccct cgcggcgcag cgtctcgtac accgccagcg
     1861 ccgccgcgct catggcgcgg aaggccgaca gggggtacaa cgcgaggcgc acgccggcct
     1921 cgcccagttc ccgcgtcgtg aacagcggcg tcttgccgaa ctccgtgatg ttggccagca
     1981 ccggcaccga cacggcggcg gtgaactggc ggtactcgtc gagcgtggtg agcgcctcgg
     2041 cgaagatcat gtccgcgccg gcctcgacgt aggcccgggc ccgctcgatc gcctgggcct
     2101 ggccctcgac ggcgtgggcg tcggtgcgcg ccatgatgac gaatgcgtcg tccgtgcgac
     2161 catccactgc ggcgcggatg cggtcgcaca tctcctggct ggagaccagc gccttgcccg
     2221 gccggtggcc gcagcgcttg gccgacacct ggtcctccag gtgcagcgcg ccggcgcccg
     2281 cgcggatcat ttcctgcacc gtgcgggcga tgttgaaggc cgcgccccag cccgtgtcgg
     2341 catccaccag cagcggcagt tccgtcgcgc cggtgatgcg ccggatgtcc tcgcagacgt
     2401 cgttgaggct ggtcatgccc aggtcgggga gaccgaagga ggcattggcc accccggcgc
     2461 cggacaggta gatcgcccgg tagcctgccc gctcggccag cagggcggtg taggcattga
     2521 tggtgccgac cacctgcagg gggcgttcga cctcgagggc ggcccggaag cgggcgccgg
     2581 cggactgggc ctgggacatg agcgacttcc tgtgctgcgg ggcgctgccg tgtgggcaac
     2641 gcatcatttt agcaccggca tcaggctgca tcagcgtacc cgcgcggccc ggacggacgg
     2701 ccggtgcccg gcggtccgcg ccgccgcgag gcctcgctca gcgctgcagg tcgacgagcg
     2761 tccggccgcg caccgtcccg tcgatatatt cgtcgaaaac accgggaagc tcggacaaac
//
LOCUS       DAOMPG010000281         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14376_length_2819_cov_4.902677, whole genome shotgun sequence.
ACCESSION   DAOMPG010000281 DAOMPG010000000
VERSION     DAOMPG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14376_length_2819_cov_4.902677"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..91
                     /locus_tag="PLS34_11895"
     tRNA            3..91
                     /locus_tag="PLS34_11895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Leu,seq:tag)
     gene            175..1482
                     /gene="tig"
                     /locus_tag="PLS34_11900"
     CDS             175..1482
                     /gene="tig"
                     /locus_tag="PLS34_11900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRP88203.1"
                     /translation="MVSVESTQGLERRMKVEVPAERVESEIDKRLKDMGRRAKIKGFR
                     PGKAPLKVVRQQYGAQVREDVVAEFVRNSWVQAISEQKLRPVGSPRIEEHNVSKDEGL
                     SFTAVFEVFPDIELTGHEGLEIERPAAEITGADVDAMIDKLRGQRMAWDEVERAAGDG
                     DRVTIDFSGAIDGEAFRGGDGNEVGIVLGEGRMLKDFEAGLQGIKPGEERTIGVEFPA
                     DYGSADVAGKKAEFKVKARKVEQSRLPEVDEEFCRAFGVEEGGVELFRAEVEENMREE
                     LQQRVRESVKRQLLDKLVAAHDIDVPVALVEQEIQSLREDMVRRMNPNAKPEGATELP
                     PREPFEQPARFRVALGMLIGEIVRQHELRVDQAQVQQRLQQIGASYGDAETVIKIYRS
                     NPELMSQVETSVMEEQVVDWLLERAKIVDKPIKFSQLMNQDQA"
     gene            1567..2187
                     /gene="clpP"
                     /locus_tag="PLS34_11905"
     CDS             1567..2187
                     /gene="clpP"
                     /locus_tag="PLS34_11905"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRP88204.1"
                     /translation="MTTKALNLVPMVVEQTARGERAYDIYSRLLKERVIFVVGPIEDH
                     MANLVVAQLLYLESENPDKDIHLYINSPGGVVTAGMSIYDTMQFVKPDVSTICVGQAA
                     SMGAILLAGGAKGKRFCLPHSRIMIHQPLGGFQGQASDIDIHAREVLKIRDRLNEILS
                     HHTGQPKEKVAQDTDRDFFMGGDESVAYGLVDRMLTRRGEALPLQG"
     gene            2295..>2819
                     /locus_tag="PLS34_11910"
     CDS             2295..>2819
                     /locus_tag="PLS34_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HRP88205.1"
                     /translation="MADDSRGKHEDGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVE
                     LCNDIIREELEDKSEPGKSKLPRPHEINAVLDQYVIGQAAAKKVLSVAVYNHYKRLEN
                     RGSDRGRGDEVELAKSNILLIGPTGCGKTLLAETLARLLNVPFTIADATTLTEAGYVG
                     EDVENIIQKLLQKCD"
BASE COUNT          545 a          879 c          982 g          413 t
ORIGIN      
        1 gcgcgaaagt ggcggaactg gtagacgcgc cgggtttagg tcccggtggg gttgatcccc
       61 cgtgagagtt cgagtctctc ctttcgcacc agattctgca ttcgcccgac ggctaatccg
      121 gctgcgcagc ggtccttcgc ggcgcgtctt aattgaaaga ggaacgacac agtgatggtt
      181 tccgtggaat ccacccaggg gctggagcgg cgcatgaagg tcgaagtgcc ggccgagcgc
      241 gtcgaaagcg aaattgacaa gcgcctcaag gacatgggcc ggcgcgcgaa gatcaaggga
      301 ttccgcccgg gcaaggcgcc gctgaaggtc gtgcgccagc agtacggcgc gcaggtccgc
      361 gaggacgtgg tcgcggagtt cgtgcgcaac agctgggtgc aggccatcag cgagcagaag
      421 ctgcgcccgg tgggcagccc gcgcatcgag gagcacaacg tctccaagga cgaggggctc
      481 agtttcacgg ccgtcttcga ggtgtttccc gacatcgagc tgaccggcca cgagggcctc
      541 gagatcgaac gccccgccgc cgagatcacc ggcgccgacg tggacgccat gatcgacaag
      601 ctgcgcggcc agcggatggc atgggacgaa gtggagcgcg cggccggcga cggcgaccgg
      661 gtgaccatcg atttcagcgg cgccatcgac ggcgaagcct tccggggcgg cgacggcaac
      721 gaggtcggca tcgtgctcgg cgagggccgc atgctgaagg acttcgaggc cggcctgcag
      781 ggcatcaagc cgggcgagga gcgcaccatc ggcgtcgagt tcccggcgga ttacggctcg
      841 gccgacgtgg ccggcaaaaa ggccgaattc aaggtcaagg cccgcaaggt cgagcagtcc
      901 cggctgccgg aagtcgacga ggagttctgc cgcgcgttcg gcgtcgagga aggcggcgtg
      961 gagctcttcc gggccgaggt cgaggagaac atgcgcgagg agttgcagca gcgggtgcgc
     1021 gagtccgtca agcgccagct gctcgacaag ctggtggcgg cccacgacat cgacgtcccc
     1081 gtggccctgg tggaacagga gatccagagc ctgcgcgagg acatggtgcg gcgcatgaat
     1141 cccaacgcca agccggaggg cgccaccgag ctgccgccgc gcgagccctt cgagcagccg
     1201 gccaggttcc gggtggcgct cggcatgctg atcggcgaga tcgtccgcca gcacgagctc
     1261 agggtcgacc aggcccaggt gcagcagcgc ctgcagcaga tcggcgcgag ctacggggat
     1321 gccgagacgg tgatcaagat ctatcgcagc aatccggaac tgatgtccca ggtggagacc
     1381 tcggtgatgg aggagcaggt cgtggactgg ttgctcgagc gcgcgaaaat cgtcgataaa
     1441 cccatcaagt tcagtcagtt gatgaaccag gaccaggctt gaggcgcagt gcgcttgcgc
     1501 ccggttcccg gttcggatac ttgtaggcag gcagcgccgc gaccgcgcgg cagcggagag
     1561 taaggaatga cgaccaaggc attgaatctc gtccccatgg tggtggagca gacggcgcgg
     1621 ggcgagcggg cctacgacat ctattcccgg cttctcaagg agcgggtcat tttcgtcgtc
     1681 gggcccatcg aggaccacat ggcgaacctg gtcgtcgccc agctgcttta cctcgagtcc
     1741 gagaacccgg acaaggacat tcacctgtac atcaactcgc cgggcggcgt cgtgaccgcc
     1801 ggcatgtcga tctacgacac catgcagttc gtcaagcccg acgtctcgac catctgcgtc
     1861 ggccaggcgg ccagcatggg cgcgatcctg ctggccggcg gcgccaaggg caagcgcttc
     1921 tgcctgccgc actcgcgcat catgatccac cagccgctgg gcggcttcca gggccaggcc
     1981 tcggacatcg acatccacgc ccgcgaggtg ctgaaaatcc gtgaccggct caacgagatc
     2041 ctttcgcacc ataccgggca gccgaaggaa aaggtggccc aggacacgga ccgggacttt
     2101 ttcatgggcg gcgacgaatc ggtggcctac gggctggtcg accgcatgct gacccgccgg
     2161 ggcgaggcgc tgcccctgca gggctgagtc cggacagcgg cgcgctttgc agccggtgtt
     2221 ggccccatgc tatataaacc ctgccgctaa gggcctgagc ccccggcaaa agtccagaat
     2281 cccgaggtcc acgcatggct gacgactccc gcggcaagca cgaagacggc aagctgctgt
     2341 attgctcctt ctgcggcaag agccagcacg aggtgcgcaa gctcattgcc ggcccctccg
     2401 tgttcatctg cgatgagtgc gtcgagctgt gcaacgacat catccgcgag gagctcgagg
     2461 acaagagcga gcccggcaag agcaagctgc cgcggccgca cgagatcaat gcggtcctcg
     2521 accagtacgt gatcggccag gcggcagcca agaaggtgct gtcggtggcg gtctacaacc
     2581 actacaagcg gctcgagaac cgcggcagcg accgcggccg cggcgacgag gtggagctgg
     2641 cgaagagcaa catcctgctg atcggcccga cgggctgcgg caagacgctg ctggcggaga
     2701 cgctggcgcg gctgttgaac gtgccgttca ccatcgccga cgccacgacc ctcaccgagg
     2761 cgggctacgt cggcgaggac gtcgagaaca tcatccagaa gctgctgcag aagtgcgac
//
LOCUS       DAOMPG010000282         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14430_length_2811_cov_2.625181, whole genome shotgun sequence.
ACCESSION   DAOMPG010000282 DAOMPG010000000
VERSION     DAOMPG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14430_length_2811_cov_2.625181"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLS34_11915"
     CDS             complement(<1..432)
                     /locus_tag="PLS34_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRP88206.1"
                     /translation="MHQPFQQTLTVRHLTLAHPLRWLRAGWRDFLQSWPVGLAHGAAV
                     ALFALLVLIVAGDHFWLLTGAFSGFLLVAPVVAVGLYAVSRELGAGRPGGFRAVLDAW
                     ISWRGQPRHDWRLVVFGCLLCLAGTGWVLTSAALITALSPAP"
     gene            complement(455..2428)
                     /locus_tag="PLS34_11920"
     CDS             complement(455..2428)
                     /locus_tag="PLS34_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRP88207.1"
                     /translation="MWTIDNFPSARVAEKYGIDIDAAWLRHAQLATTRLESGCTGSFA
                     SPDGLMLTNNHCVWACIRHLSSDERNLSDEGFLAGSRGEELQCPGQQASVLVALEDVT
                     AKVARAIAGLDARKANEARKAELTRLESACEAASGKTLSCEAVPLYNGGLYFMHQYKR
                     YDDVRLVFAPELDIGAFGGDPDNFQFPRWSLDMTFLRAYENGKPAATPHFFAWRRGGP
                     AAGEPVLLTGHPGRTDRALTVSQLRHLRNIQWPHYLLRTAELRGRLLEWQKTGPEAAR
                     IVQQRILNIENGIKVRYQQLRALQNDEMMAIKAANERALRERAPSEEPWELIDGAMAA
                     YANMYDEYLFIEAGAAFQGDLFSYARTLVRGTAERERPDSERMREYTDAALRKVEQRL
                     LADRPVSKAYDELVLAFSLEKMRQWLGPDSPHVHEVLGDASPEALAARLVAGTRLADP
                     AYRKTLLEGGAAAVRASEDPMIRFALAIDPAARAIRKRYEDEVEAPRIRGESMIADLR
                     FEVYGTDTYPDANFTLRVTYGAVEGWDEKGEMVDPITRTRRLFERATGERPFRLPDSW
                     AEAQDQLDPDTPFNFVATTDITGGNSGSPILAADGALVGVAFDGNIHSIAGDYWFDPA
                     TNRTVGVNSAIMLEALDTVYGAAWLLEELTVVD"
     assembly_gap    2559..2591
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          432 a          947 c          956 g          443 t
ORIGIN      
        1 gggcgcgggc gacagcgcgg tgatcagcgc cgccgaagtc agcacccagc cggtgccggc
       61 caggcacaac aggcagccga acaccaccag ccgccagtca tggcgcggct gcccgcgcca
      121 gcttatccac gcatcgagca cggcccgaaa gccgcccggc cgcccggcgc cgagctcccg
      181 gctcaccgcg tacagcccca ccgcgaccac cggcgccacc agcaggaagc cgctgaacgc
      241 gccggtcagc aaccagaaat gatcgcccgc gacgatgagc accagcagcg caaacagcgc
      301 caccgccgcg ccatgcgcca ggccgaccgg ccagctctgc aggaaatcgc gccagccggc
      361 gcggagccag cgcagggggt gggcgagggt gaggtggcgg acggtgaggg tttgctggaa
      421 gggttggtgc attgggggat tccttgccga ccagtcagtc tacgaccgtg agctcctcca
      481 gcagccacgc ggcgccatac acggtgtcca gcgcttccag catgatggcg gaattgaccc
      541 ccaccgtccg gttggtggcc gggtcgaacc agtagtcgcc ggcgatcgag tggatgttgc
      601 cgtcgaaggc caccccgacc agggcgccgt ccgccgcgag gatcgggctg ccggagttgc
      661 cgccggtgat gtccgtggtg gcgacgaagt tgaacggcgt gtcgggatcg agctggtcct
      721 gcgcttcagc ccagctgtcg ggcagccgga acggccgctc gccggtggcg cgctcgaaca
      781 gccggcgggt gcgcgtgatg ggatcgacca tttcgccttt ttcgtcccag ccctcgaccg
      841 cgccataggt cacgcgcagc gtgaagttgg cgtccgggta ggtgtcggtg ccgtacacct
      901 cgaagcgcag gtcggcgatc atgctttcgc cgcggatccg cggcgcttcg acctcgtcct
      961 cgtagcgctt gcggatggcg cgcgccgccg gatcgatggc gagcgcaaac cggatcatcg
     1021 ggtcctcgct cgccctgacc gcggccgcgc cgccttccaa cagggtcttg cggtaggccg
     1081 gatccgccag gcgggtgccc gccacgagcc gggccgcgag cgcctccggc gaggcgtcgc
     1141 cgagcacctc gtgcacgtgc gggctgtccg gtcccagcca ctgccgcatt ttctccagcg
     1201 agaaagccag caccagctcg tcataggcct tgctcaccgg ccgatccgcc agcaggcgtt
     1261 gctcgacctt gcgcagcgcg gcgtcggtgt actcgcgcat gcgctcgctg tccggccgct
     1321 cgcgctccgc cgtgccgcgc accagggtgc gcgcatagct gaacaagtcg ccctggaacg
     1381 ccgcgcccgc ctcgatgaaa aggtactcgt cgtacatgtt ggcgtacgcc gccatcgcgc
     1441 cgtcgatgag ctcccagggc tcttcgctgg gggcacgctc gcgcagcgcg cgctcgttcg
     1501 cggccttgat ggccatcatc tcgtcgttct gcagcgccct gagctgctgg tagcgcacct
     1561 tgatgccgtt ctcgatgttg aggatgcgct gttgcacgat gcgcgccgcc tccgggccgg
     1621 tcttctgcca ctccagcagg cgcccgcgca gctccgccgt ccgcagcagg tagtgcggcc
     1681 actggatgtt gcggaggtgc cgcagctgcg acaccgtcag cgcgcgatcg gtcctgcccg
     1741 gatggccggt gagaagcacc ggctccccgg ccgccgggcc gccccggcgc caggcgaaga
     1801 aatgtggcgt ggccgcgggc ttgccgttct cgtacgcccg caggaaggtc atgtcgagac
     1861 tccagcgcgg gaactggaaa ttgtccgggt cgccgccgaa ggcgccgata tccagctccg
     1921 gcgcaaacac caggcggaca tcgtcatagc gcttgtactg gtgcatgaaa tacagcccgc
     1981 cgttgtacag cggcaccgcc tcgcaactga gcgtcttgcc cgaggcggcc tcgcaggccg
     2041 actccagccg cgtcagctcc gccttgcgcg cctcgttggc cttgcgcgcg tcgaggcccg
     2101 cgatcgcgcg cgcgaccttc gccgtcacgt cctccagcgc caccagcacc gaagcctgct
     2161 ggcccgggca ctgcaactcc tcgccgcggc tgcccgccag gaagccctcg tcggacaggt
     2221 tgcgttcatc gctgctgaga tgccggatgc aggcccaaac gcagtggttg ttcgtcagca
     2281 tgagcccatc cggcgaagcg aacgaccccg tgcagccgct ctccagccgc gtggtcgcga
     2341 gctgcgcatg gcgcagccac gccgcatcga tgtcgatgcc atatttttcc gccacgcgag
     2401 ctgagggaaa gttatcgatg gtccacatgc cctcgtcggc ggaggcggag ggggacaagg
     2461 acagtgcgag gcatggcagg atggtggctg ggaggatttt tcgcatggga aattgctgga
     2521 gagagtgggg aggtgggtat tttactgtgt ttgggggann nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn ngagacgcga gcgggccgga atgtgaacgc gggctcatac cgtgagcccc
     2641 cgaccggtta cggtcgggag cgcactgcct attgaatcaa gtgccgatat tgggctgaat
     2701 ggcccagaaa tgcgcgcgtg ggggagggat ggactgccct ttttgtgatc caccgaagga
     2761 ccgggtgttc tacgaggacg agctcgtgct cggcctgtgg gatgcgtttc c
//
LOCUS       DAOMPG010000283         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14902_length_2740_cov_3.785475, whole genome shotgun sequence.
ACCESSION   DAOMPG010000283 DAOMPG010000000
VERSION     DAOMPG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14902_length_2740_cov_3.785475"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLS34_11925"
     CDS             <1..410
                     /locus_tag="PLS34_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HRP88208.1"
                     /translation="VAFLEEMYGNLPNRWSPELCGVERWRFIVNAFTRLRFLRPDGSL
                     DFGSKGPAESARSDLIPWFRYPARRSAGMRIVFGHWSLLHGGVFDGAFAIDTGCVYGG
                     SLTALNLGDLSTRCIDCAPGWHPKRRSDTRKSG"
     gene            440..1222
                     /locus_tag="PLS34_11930"
     CDS             440..1222
                     /locus_tag="PLS34_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP88209.1"
                     /translation="MKLSLEGRRAVVCGASRGIGRAIAGVLADMGAQLTLVARDVDAL
                     RGVADKLPGGAARHLVRPLDVANTADVEAAFAEIATEGPVHVLINNTGGPAAGTAFDA
                     RPDDFVQAFRQHLVTAQIAAQALVPGMKKAGYGRIINITSTSVKEPIPGLGVSNTIRA
                     AVASWAKTLSRELAPHGITVNNLLPGFTSTERLDYLFRQRADKSGGSVEEIERAALAQ
                     VPAGRFARPEEIAWAVGFLASPQAAYVNGINVPVDGGRMASL"
     gene            1249..2645
                     /locus_tag="PLS34_11935"
                     /pseudo
     CDS             1249..2645
                     /locus_tag="PLS34_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
BASE COUNT          408 a          971 c          949 g          412 t
ORIGIN      
        1 tcgtcgcttt tctcgaggag atgtacggca accttccgaa ccgctggtcg ccggaattgt
       61 gcggtgtcga gcgatggcgc ttcatcgtta acgcctttac gcggttgcgt ttcctgcgtc
      121 ccgacggcag cctcgacttc ggctcgaagg ggccggccga gagcgcccga tccgacctga
      181 ttccctggtt ccgctacccc gccagacgca gcgcggggat gcggatcgtc ttcggccact
      241 ggtcgttgct ccatggcggc gtattcgacg gcgcgttcgc catcgacacg ggctgcgtct
      301 acgggggaag cctgacggcg ttgaatctcg gcgacctgtc cacccggtgc atcgattgcg
      361 cgccgggctg gcatccgaag cgtcgctccg acacgcgcaa gtccggttag aatcagcagg
      421 cacagcagga ggttgcagca tgaaactgag tctcgagggc cgccgcgccg tggtgtgcgg
      481 cgccagcagg ggcatcgggc gcgccatcgc cggcgtgctg gcggacatgg gagcgcagct
      541 gaccctggtg gcacgcgacg tcgacgcgct ccgcggggtt gcggacaagc tgccgggcgg
      601 cgcggcgcgg caccttgtcc ggccgctcga cgtggcgaat acggcggacg tcgaggcggc
      661 attcgcagag atcgcgaccg agggcccggt gcacgtgctg atcaacaaca ccggcgggcc
      721 ggcggcgggc acggccttcg acgccaggcc cgatgatttc gtgcaggcct tccgccagca
      781 tctcgtcacg gcgcagatcg ccgcccaggc gctggtgccg gggatgaaga aggccggcta
      841 tgggcggatc atcaacatca cctcgacctc cgtgaaggag cccatccccg gcctcggcgt
      901 ctccaacacc atccgcgcgg cggtggccag ctgggcgaag accctctccc gcgagctcgc
      961 cccgcacggc atcaccgtca acaacctatt gccgggcttc accagcaccg agcggctcga
     1021 ttacctgttc cggcagcgcg ccgacaagtc gggtggcagc gtcgaggaga tcgagcgcgc
     1081 ggcactggca caggtgcctg ccggccgctt cgcacgcccg gaggaaatcg cctgggcggt
     1141 cggcttcctg gcgtcgccgc aggcggccta cgtcaacggc atcaacgtgc cggtggacgg
     1201 cgggcgcatg gcgagcctgt gagccggcgc gtcgaagtca acaactacat cgacggccgc
     1261 ctgcaggcgc cggcgggcga tgcctggatt gacctctacg agcccgccac cgggcaatcc
     1321 tgcgggcgcg tcgcggacag cgatgccaac gacctcgagc aggctgtggc cgctgcgcag
     1381 caggcccagg ccgactggtg caccgccgga gccgaggcgc gcgcgagctg gctgcacacc
     1441 ctggcgaacc tggtggaggc gcgcgccgaa cagctggccg ccgccgaatc cgccgacacc
     1501 ggcaagccgc tggcactggc gcgcaagctc gacatcccgc gggtggcggc caacctgcga
     1561 ttctttgcag cagcggccac ccagttcgcc agcgagtcgc atgccatggg cgcggccgcc
     1621 atcaattaca cgctgcgctc cccgctcggg gtcgtcggct gcatctcgcc ctggaacctg
     1681 ccgctctacc tgctgagctg gaaggtggca ccggcgctgg cggcgggcaa ctgcgtcatc
     1741 gccaagccgt cggagctgac gccggtgtcc gccggcctgt ttgccgagct gtgcatcgag
     1801 gccgggctgc ccccgggcgt gctgaacatc ctgcacggcc gcggtcccgg catcggccgg
     1861 ctgctgaccg agcatccggc ggtacgagcc atctcgttca ccggcggtac gcgcaccggc
     1921 caactgatcg cacaagccgt cgcgcccacc ttcagaaagc tttcgctgga gctgggcggc
     1981 aagaatccga gcatcgtgtt cgccgactgc gacttcgagc gcaccgtcga aggcgtcgcg
     2041 cgcgccgcct tcaccaacca gggtcaggtc tgcatgtgcg gctcgcgcat cctggtggaa
     2101 gccccgctct acgagcgctt ccgcgacgcg ctggtggcgc gcgtcggccg gctgaaagtc
     2161 ggcgatccgc tggaggccga caccgaccag ggcgcgctgg tatccgcgga tcatcagcgc
     2221 aaggtgctcg agcacatcgc cctggcgcgg gccgctgggg gccggctgct gtgcggtggc
     2281 gaggccgtcg ttccggcggg cgagcgctgc cgcgatggct ggttcgtcgc gcccacattg
     2341 ttcgaacagc tgccgccggc ccagtcgcca cgctgctgcc cttcgacacc gaggcacagg
     2401 cggtcgagct ggccaattgc gtgccctacg gcctcgcggc gtccatctgg agccgcgaca
     2461 tcggccgctg ccaccgcgtg gcggcgaaca tcgaggccgg cgtcgtgtgg atcaactgct
     2521 ggatgctgcg cgacctgcgc acgccgctgg ggggcaccaa gcaatccggc atcggccgcg
     2581 agggcggctt cgaggccatg cgcttcttca ccgaacccaa gaacgtctgc gtgagctacg
     2641 actgacatga gcggcagcac cacgaccagc accgcgccgc cccctgtcgg ccattatccc
     2701 catgcgcgcc gcgccggcgg actgctgttc ctgtccgggc
//
LOCUS       DAOMPG010000284         2734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_14943_length_2734_cov_3.923479, whole genome shotgun sequence.
ACCESSION   DAOMPG010000284 DAOMPG010000000
VERSION     DAOMPG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_2734_cov_3.923479"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..770)
                     /locus_tag="PLS34_11940"
     CDS             complement(111..770)
                     /locus_tag="PLS34_11940"
                     /inference="COORDINATES: protein motif:HMM:NF017819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRP88210.1"
                     /translation="MHDQRIAEDILDYWFGDAVRDPSKVPGRMGFWFNVDPQRDAEMR
                     ERWGELVAAASEGKLDFWTRTPRGRLAVLILLDQMRRNIYRGTADAFRHDGRARYLMR
                     DGVSRLMDMKLTPIERVFFYMPLQHAESLDDQELAVDRFLQLEREVASDHREIFAGFR
                     KYAQKHRDIVARFGRFPHRNAILGRRDTPEEAAWLAGGGERFGQSTPTDATGNYKALP
                     G"
     gene            893..1381
                     /locus_tag="PLS34_11945"
     CDS             893..1381
                     /locus_tag="PLS34_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88211.1"
                     /translation="MQANWTKRDAGPSAAAPLASWLAWLLAAALLGGCSKPVDAPEAR
                     LRALLAEAEQAAEAGDHQALAAWVARDYADSAGRDRRTVALTLRGLLMRYPRLELIVT
                     VREIQLHSPQLATVRLEILSAGAGASGLAADAFGLELSLRDDGGWKVTRAEWRGRTGR
                     GI"
     gene            1558..>2734
                     /gene="gltB"
                     /locus_tag="PLS34_11950"
     CDS             1558..>2734
                     /gene="gltB"
                     /locus_tag="PLS34_11950"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630496.1"
                     /note="catalyzes the formation of glutamate from glutamine
                     and alpha-ketoglutarate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRP88212.1"
                     /translation="MSAEQGLYRPAFERDSCGFGLIASLAGRDSHWLVATAVAALGRL
                     THRGAVAADGKTGDGCGILLSAPESFLRTVAEQAGLAPGAHFAAGMVFMDHDPGRAAA
                     ARNTIRACVAAEGLRLDGWRTVPVNPEACGEQALRTLPLIQQVFVTAPEAMPTDTFRR
                     RLFVARRRAEQKLEASDPVFYIASLSPDTVAYKAMVMPEQLPVFYPDLADERLAARVC
                     VFHQRFSTNTLPQWRLAQPFRYLAHNGEINTIQGNRNWALARAPKFRSQALPELQHLT
                     PLVSLSGSDSSSLDNMLEVLVAGGMDMMQAARLLIPPAWQTVDDIDSDTRAFYEYYGM
                     HLEPWDGPAGIVMTDGRNAACILDRNGLRPARWVVTDDDHLTIASEVGVWDYRPEQVV
                     "
BASE COUNT          392 a          931 c          980 g          431 t
ORIGIN      
        1 tatcctggag gcgcgcatcg acgcctggct ggcggcgcag gcggacggcg gccggccctg
       61 atcctgtcgg ccggacgcgc gtcgttggca gcgcgggccc ggccggggcc tcagcccggc
      121 agcgccttgt agttcccggt agcgtcggtc ggcgtcgact gtccgaagcg ctccccgccg
      181 ccggccagcc aggcggcctc ctcgggcgtg tcgcgacgcc cgagaatcgc gttgcggtgc
      241 gggaagcggc cgaagcgcgc gacgatgtcg cggtgcttct gcgcgtactt gcggaagccg
      301 gcgaaaatct cgcggtggtc ggaggcgacc tcgcgctcca gctgcaggaa gcggtccacc
      361 gccagttcct ggtcgtcgag cgattccgcg tgctgcagcg gcatgtagaa gaacacccgc
      421 tcgatcggcg tcagcttcat gtccatcagg cggctgacgc cgtcgcgcat caggtagcgg
      481 gcgcgcccgt cgtgccggaa cgcgtcggcg gtgccgcggt agatgttgcg acgcatctgg
      541 tcgagcagga tcagcaccgc cagccgcccg cgcggcgtcc gggtccagaa gtcgagcttg
      601 ccctccgagg cggcggccac cagctcgccc cagcgctcgc gcatctccgc gtcgcgctgc
      661 gggtccacgt tgaaccagaa acccattcgg ccgggtacct tcgaagggtc ccggacggca
      721 tcgccgaacc agtagtcgag aatgtcttcg gcgatgcgct gatcgtgcat tggctgctgt
      781 cctcgtgccg ggctgcggcg agcgaatcgg ctcgccgcaa atatcggctt tcatgccgcg
      841 ggagtccagt ctacgctggg ccctcacccc ggaacgatcg tggtgtccac gcatgcaagc
      901 taattggacg aaacgggatg cgggtccgtc cgcggcggcg cccctggcgt cctggctggc
      961 atggctgctg gccgcggcgc tgctgggcgg ctgcagcaag ccggtggacg cgccggaggc
     1021 gcggctacga gccttgctcg ccgaagcaga gcaggccgcc gaggcgggtg atcaccaggc
     1081 gctcgcggcg tgggtggcga gggattacgc ggacagcgcc ggacgcgacc ggcgcacggt
     1141 ggcgctgacg ctgcggggat tgctgatgcg ctatccgcgg cttgagctga tcgtgaccgt
     1201 gcgtgaaatc cagctgcatt cgccgcagct ggcgaccgtg cggctggaga tcctgtcggc
     1261 cggcgccggc gcgtccggcc tggcggcgga cgccttcggg ctggaactct cgttgcggga
     1321 cgacggcggc tggaaggtga cgcgcgccga gtggcgaggc cggaccggcc gcggcatttg
     1381 accttggcgc cggagtctgt tttatagtct gtgttgcact gcgcacaaaa accgcctgtg
     1441 cataagtgat caaataaaaa caagcacttg aagtggtcgg cccgtgttcc gggccgggtt
     1501 ttggatatcc agggcaaccg ggatcggttc ggccgagggc cggagggagt cttgaccgtg
     1561 tctgccgagc agggcctcta tcgaccggct ttcgagcggg acagctgcgg cttcggcctg
     1621 atcgccagcc tcgcgggccg cgacagccac tggctggtcg ccacggccgt cgcggcgctg
     1681 ggccggctga cccaccgcgg cgccgtggcg gccgacggca agaccgggga tggctgcggc
     1741 atcctgctgt cggcgcccga atcctttctg cgcacggtag ccgagcaggc cggcctcgcg
     1801 cccggcgcgc acttcgcggc cggcatggtg ttcatggacc acgacccggg gcgcgccgcg
     1861 gcggcgcgca ataccattcg cgcctgcgtg gcggctgaag ggctgcgcct ggacggctgg
     1921 cgcaccgtgc cggtcaatcc cgaggcctgc ggcgagcagg cgctcaggac cctgccgctg
     1981 atccagcagg tattcgtcac cgcgccggag gccatgccga ccgacacctt tcgccgccgg
     2041 ctgttcgtgg cgcgccggcg tgccgagcag aagctcgagg ccagcgatcc ggtgttctac
     2101 attgccagcc tgtcgcccga cacagtggcc tacaaggcca tggtgatgcc ggagcagctg
     2161 ccggtgttct atccggacct ggccgacgag cggctggcgg cgcgcgtctg tgtctttcac
     2221 cagcgtttct cgaccaacac gctgccgcag tggcgcctgg cgcagccctt ccgttacctc
     2281 gcccacaacg gcgagatcaa caccatccag ggcaaccgca actgggcgct ggcgcgggcg
     2341 ccgaaattcc gctcgcaggc gctgcccgag ctgcagcacc tcacgccgct ggtgtcgctg
     2401 agcggctccg actcctccag cctcgacaac atgctggagg tgctggtggc cggcggaatg
     2461 gacatgatgc aggcggcccg cctgctgatc ccgcctgcct ggcagaccgt ggacgacatc
     2521 gattcggaca cgcgcgcctt ctacgagtat tacggcatgc acctcgagcc gtgggacggc
     2581 ccggcgggca tcgtcatgac cgacgggcgc aacgccgcct gcatcctcga ccgcaacggc
     2641 ctgcgtccgg cacgctgggt ggtgaccgac gacgaccacc tgaccatcgc ctccgaggtc
     2701 ggagtgtggg actaccggcc cgaacaggtg gtcg
//
LOCUS       DAOMPG010000285         2724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15007_length_2724_cov_3.062196, whole genome shotgun sequence.
ACCESSION   DAOMPG010000285 DAOMPG010000000
VERSION     DAOMPG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15007_length_2724_cov_3.062196"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..494
                     /locus_tag="PLS34_11955"
                     /pseudo
     CDS             1..494
                     /locus_tag="PLS34_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
     gene            complement(515..889)
                     /locus_tag="PLS34_11960"
                     /pseudo
     CDS             complement(515..889)
                     /locus_tag="PLS34_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
     gene            complement(954..>2724)
                     /locus_tag="PLS34_11965"
     CDS             complement(954..>2724)
                     /locus_tag="PLS34_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRP88213.1"
                     /translation="ELRLRPKSFDLLLYLLRHPGRLVGREELLNAVWGQLAVTDDSIT
                     QCLVEIRRALGDESRSMVRTVPRRGYRLEVPVDTPAGRDARPDAGEKPSRLPVKRTHA
                     AAALVGMALFAALAWWGSARHAQDPAPRATPGGNAVAVLPFADMSEAGDQDYFGAGIA
                     EEILNLLAQLDSLKVIARTSSFAFQGQAMDVRDIARRLGVTHVLEGSVRRSGDRMRIT
                     AQLVDGRDGVHLWSQTYDREVGDIFDLQLEIAQQVSGVLAATLHVESPARHRDVRAYE
                     HLLRGRFLFQRRAPGDMERAREHFEAAVALDPEDGAAWAALSGCLTVDMFEGRLEVAE
                     ALPRALEAAQRAVALSPGLPEAHLRAARAYGFAGDEERAWRHIGIAEALNPDDPLLLG
                     FQAGAALARDDLAGALALQERVVALDPLSVVIRNNYAYMLQAAGRLAEARAQLQTGLE
                     ISPGRHDFTVALARVLVLEGRHEEAIAGLPEASPGGDADVVRAVALHALGRVEEAQAA
                     AGRLETGTSADTAMYLAEVHAQLGDVEAAWRWLDEARRRMQAEMRGDGWRWRQRVRSS
                     GFLRPLESDPRWQAAFRDPPDAT"
BASE COUNT          408 a         1001 c          924 g          391 t
ORIGIN      
        1 acaccaagct cgagctgttc caggatcagg tgttctgctt ctcgcccaag ggccgcttga
       61 tcgcgctccc acgaggcgcg acaccgctcg atttcgccta cgccgtgcac accgacatcg
      121 gcaatcgcgc cgtgggtgcc tacgtcaacg gccgccatgt gccgatcgac acgcgcttgc
      181 gcaacggtga cgaggtggag atccagacat cgaaaacgca cacgccgccc gccgcatggg
      241 aaagccttgc ggtgacgggc cgcgcccgct ccgccatccg ccgcgccgca cgcgatgcga
      301 tccgccgcca gtatgcggaa ctcggccgcc gtctcctcat ctcggctttc gatcgcatcg
      361 gggaagctta caccgacgac cgcttgcgca aggctttgcc gcgcctctcc cagaagtcgg
      421 tggatgatgt cctggcggcc gtcgcgcgca acgagctcaa ggtggaagac gtggtgcgtg
      481 ccgtcgcccc ggaggttgcg gccgctgcgc gcgcgatgtc tttcaggccg gtgtcgccgc
      541 tgacgggctt tgactgctcg cggacgaact tcatgccgtt ccagtccggc gggttgtggc
      601 tggcggtgac catgatgccg ccgtcgacgc cgaggtggct ggtggcgaaa tagaccagct
      661 cggtgccgca cagcccgatg tcgagcacgt ccacgccggc ggccatcagt ccgtcggcca
      721 gcgcatctgc cagctccggc gaggacggcc gcacgtcgta gccgacgacc accgtccgcg
      781 gccggatcac cgccgcatag gccaggccga tgcgccgcgc gatgtcgggg ttgagatcgt
      841 cgggcaggcg cccgcgcacg tcataggcct tgaagcactt cagggtcatc gccgctgggt
      901 cctcgcacgg tcggggtttc gccggcgaca tgatgccacg aaccgtggcc ccgtcaggtc
      961 gcgtcgggcg ggtcgcggaa agcggcctgc cagcgcgggt cgctctccag cggccgcaga
     1021 aagccggatg agcgcacccg ctggcgccag cgccagccat cgccacgcat ctcggcctgc
     1081 atccgtcggc gcgcttcatc gagccagcgc caggccgctt cgacgtcgcc gagctgcgca
     1141 tgaacctccg ccaggtacat ggccgtatcg gcggatgtgc cggtctcgag ccggcctgcc
     1201 gccgcctgtg cttcttcgac gcgacccagc gcatgcagcg cgaccgcccg cacgacgtcg
     1261 gcgtccccac cgggcgatgc ttccggcagg ccggcgatgg cctcctcgtg ccgtccctcg
     1321 agcaccagca ccctggccag cgccacggtg aagtcgtgcc gaccgggact gatctccaaa
     1381 ccggtctgca gctgggcgcg cgcctcggcc agccggccgg ccgcctgcag catgtaggca
     1441 tagttgttgc gaatgacgac cgacagcggg tccagcgcca ctacccgttc ctgcagcgcc
     1501 agcgcgccgg cgagatcatc gcgcgccagc gccgcgcccg cctggaagcc gagcagcaac
     1561 ggatcgtccg ggttcagcgc ctcggcgatt ccgatatggc gccaggcgcg ctcctcatcg
     1621 ccggcgaaac cgtacgcccg ggcggcacgc aggtgcgctt caggcaagcc ggggctgagg
     1681 gccactgcac gctgcgccgc ttccagcgcg cggggcaggg cctctgcgac ctcgaggcgc
     1741 ccctcgaaca tgtcgaccgt gaggcagccg ctcagcgcgg cccacgcggc accatcctcc
     1801 gggtccagcg cgaccgctgc ctcgaagtgc tcgcgtgccc gctccatgtc gcccggcgcg
     1861 cgccgctgga acaggaagcg gccgcgcagc aggtgctcgt aggcgcggac gtcgcgatgc
     1921 cgggcgggcg actcgacatg cagtgtggcg gcgagcacgc cgctgacctg ctgggcgatc
     1981 tccagctgca ggtcgaagat atcgccgact tcgcggtcgt aggtctgcga ccacaggtgc
     2041 acgccgtccc ggccatcgac cagctgcgcg gtgatgcgca tccggtcgcc ggagcggcgc
     2101 acgctgccct ccagcacgtg cgtgacgccg aggcgccggg cgatgtcgcg cacgtccatg
     2161 gcctggccct ggaaggcgaa ggacgaggtc cgcgcgatca ccttgagcga gtcgagctgc
     2221 gccagcaggt tgaggatctc ctccgcaatg cccgcgccga aatagtcctg gtcgccggcc
     2281 tcgctcatgt cggcgaaggg cagtacggcg acggcgttgc cgccaggggt ggcccgcggc
     2341 gcgggatcct gtgcatggcg cgcgctcccc caccaggcca atgcggcaaa caacgccatg
     2401 cccacgagcg ctgccgcagc atgcgtccgc ttcaccggca gccgtgacgg tttctcgccg
     2461 gcgtcaggcc gggcgtcgcg accggccggc gtatcgaccg gcacctccag ccggtaaccg
     2521 cgccgcggga cggtacgcac catcgaacgt gactcgtcgc cgagtgcgcg acggatctcc
     2581 accaggcact gggtgatcga gtcgtccgtc accgccagct gcccccacac ggcgttcagc
     2641 agctcctcgc gcccgaccag ccgacccgga tggcgcagca ggtacaagag gagatcgaag
     2701 ctcttcgggc gcagccggag ctcg
//
LOCUS       DAOMPG010000286         2720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15030_length_2720_cov_3.109193, whole genome shotgun sequence.
ACCESSION   DAOMPG010000286 DAOMPG010000000
VERSION     DAOMPG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_2720_cov_3.109193"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /locus_tag="PLS34_11970"
     CDS             <1..2477
                     /locus_tag="PLS34_11970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRP88214.1"
                     /translation="LVTAIRLVAPLHEFGRRLFPNGERHLRSRRRLAGLYPAPLLEAA
                     AGIARRCSFTLDELDWRYPAELVPAGETPASWLARLTAQGMARRWPGGAPAAVEQTVH
                     RELALIGELGYEAYFLTVHDIVSHARSLGILCQGRGSAANSAVCFCLGITEVDPARMS
                     VLFERFISKERNEPPDIDVDFEHERREEVIQYIYAKYGRRRAALAATVIRYRPRSAMR
                     DAGKALGLAPLQVDTLAKSMQWWDGKKDIAPERIRDAGFDPGNPRIALLIELARELLG
                     FPRHLSQHVGGFVIADDCLETLVPVENAAMPERTVIQWDKDDLDAVGMIKVDVLGLGM
                     LSAIRRSFGLIEGFRGQRWSLASVPAEDPAVYDMICAADTMGVFQIESRAQMAMLPRL
                     RPRCFYDLVIEVAIIRPGPIQGDMVHPYLRRRNGEEPVSYPSEEVRRVLERTLGVPIF
                     QEQVMQLAIVAAGFSAGEADQLRRAMAAWRRKGGLGPFEERLIEGMRRRGYAEEFARR
                     IFHQIMGFGEYGFPESHSASFALLVYVSCWLKRHEPAAFLCALINSQPMGFYAPSQLV
                     QDAQRHGVEVRAVDVLASDWDCTLEAGEAGLRLVAGLSAAGGERLVAARRARPFEHVD
                     DLARRARLDRGDLGALARAGALAALAGHRHRAAWSVAGIEAPLPLLEDAGIREGLPLL
                     RAPTEGRDIVADYRSTGLTLGRHPLALLRERLADDGLVSAAELNAAPHGAWTACAGIV
                     INRQRPGSARGVVFATLEDETGQVNVIVWKAVAEAWRRPLLGSRLLEVAGRVQREGAV
                     VHLVAERMADRSALLGALDVRSRDFH"
BASE COUNT          353 a          953 c         1011 g          403 t
ORIGIN      
        1 acctcgtcac cgccattcgc ctggtcgcgc cgctgcacga gttcggccgc cggctgttcc
       61 cgaacggcga gcgccacctg cgcagccgcc gccggctcgc cgggctgtat cccgccccgc
      121 tgctcgaggc cgcggcgggc attgcgcggc gctgcagttt cacgctcgac gagctggact
      181 ggcgctaccc ggcggagctg gtgccggcgg gcgagacgcc ggcctcctgg ctggcgcgac
      241 tgaccgcgca gggcatggcg cggcgctggc ccggcggtgc gcccgcagcg gtcgagcaga
      301 cagtgcaccg cgagctggcg ctgatcggcg agctcggcta cgaggcgtat ttcctcaccg
      361 tgcacgacat cgtcagccac gcgcgcagcc tcgggatcct ctgccagggc cgcggctcgg
      421 cggccaactc ggcggtgtgt ttctgcctcg gcatcaccga ggtggatccg gcgcgcatgt
      481 cggtgctgtt cgaacgcttc atctcgaagg aacgcaacga gccgcccgac atcgacgtgg
      541 acttcgagca cgagcgtcgc gaggaggtga tccagtacat ctatgcgaag tacggccgcc
      601 gccgcgccgc gctggccgcg accgtcatcc gctaccggcc gcgcagcgcc atgcgcgacg
      661 ccggcaaggc gctggggctg gcgccgctgc aggtggacac gctggccaag tccatgcagt
      721 ggtgggatgg caagaaggac atcgcgcccg agcgcatccg cgacgccggt ttcgaccccg
      781 gcaacccgcg catcgcgctg ctgatcgagc tggcccgcga gctgctcggc ttcccgcgcc
      841 acctgtcgca gcacgtcggc ggcttcgtga tcgccgacga ttgtctcgag acgctggtgc
      901 cggtggagaa cgccgccatg ccggagcgca ccgtgatcca gtgggacaag gacgatctcg
      961 acgcggtcgg gatgatcaag gtggacgtgc tggggctggg catgctgagc gccatccgcc
     1021 gcagcttcgg actcatcgag ggtttccgcg ggcagcgctg gtcgctggcg agcgtgccgg
     1081 ccgaggaccc ggcggtctac gacatgatct gcgcggccga caccatgggc gtgttccaga
     1141 tcgagtcgcg cgcccagatg gcgatgctgc cccggctcag gccgcgctgc ttctacgacc
     1201 tggtcatcga ggtggcgatc atccgccccg ggccgatcca gggcgacatg gtgcatccct
     1261 atctcaggcg ccggaacggc gaggagcccg tcagctaccc gagcgaggag gtgcgccggg
     1321 tgctcgagcg taccctcggc gtgccgatct tccaggagca ggtgatgcag ctggcgatcg
     1381 ttgccgccgg cttcagcgcg ggcgaggccg accagctcag gcgcgcgatg gcggcctggc
     1441 ggcgcaaggg cggactcggc cccttcgaag agcggctcat cgagggcatg cgccggcgcg
     1501 gctacgccga ggaattcgcg cggcgcatct tccaccagat catgggtttc ggtgagtacg
     1561 gtttccccga gtcgcactcg gcgagcttcg ccctgctggt ctacgtgtcc tgctggctga
     1621 agcgccacga gccggcggcc ttcctgtgcg cgctcatcaa cagccagccg atgggcttct
     1681 atgcgccctc gcagctggtg caggacgcgc agcgccacgg cgtcgaggtg cgtgccgtcg
     1741 atgtcctggc gagtgattgg gactgcacgc tggaggcggg cgaggcgggc ctgcgcctgg
     1801 tggccggcct gtctgcggcg ggcggcgagc gcctggtcgc ggcgcggcga gcgcgcccct
     1861 tcgagcatgt cgacgacctg gcgcggcgcg cccggctgga tcgcggcgac ctcggggcgc
     1921 tggcgcgcgc cggcgcgctc gcggccctgg cgggacaccg gcatcgcgcc gcctggagcg
     1981 tggccgggat cgaggcgccg ctgccgctgc tggaggatgc cggcatccgc gagggcctgc
     2041 cgctgctgcg cgcaccgacc gagggccggg acatcgtggc ggactaccgc agcaccggcc
     2101 tgacgctcgg ccgccatcca ctggcgctgc tgcgggagcg gctggccgac gacggcctgg
     2161 tcagcgcggc cgaactcaat gccgcgccgc acggcgcctg gaccgcctgc gccggcatcg
     2221 tcatcaatcg ccagcggccg ggcagcgcca ggggcgtggt tttcgcgacc ctcgaggacg
     2281 agaccggcca ggtcaacgtg atcgtctgga aagcggtggc cgaggcctgg cggcggccgc
     2341 tgctcggctc gcggctgctg gaggtcgccg gccgggtgca gcgcgaaggc gcggtggtgc
     2401 acctggtggc ggagcgcatg gccgaccgca gcgcgctgct cggcgcgctc gacgtccgct
     2461 cgcgggactt ccattaatcg gggtcagaca cctatttccg gttgtccgcg gcaccgtgcc
     2521 gtcatgggcg cgcttcgttc ggcgaagtct gagctcagcg cgcccatgac accccggtcc
     2581 cgctgccgtg cggacagcgg cagcgccttg gcttcgcggt gccgctgacg atcgcgacgc
     2641 tggcgaccgg cgttgccggg gcggactgac ctagcgtggt cgctggcgat caggccatca
     2701 cgccgactct gcagccggcc
//
LOCUS       DAOMPG010000287         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15116_length_2708_cov_4.016962, whole genome shotgun sequence.
ACCESSION   DAOMPG010000287 DAOMPG010000000
VERSION     DAOMPG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15116_length_2708_cov_4.016962"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PLS34_11975"
     CDS             complement(<1..176)
                     /locus_tag="PLS34_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP88215.1"
                     /translation="MNETSPVLSCRGLGKRFVEGGNVVAVLNGVELDVMPGDRLAIVG
                     ASGSGKTTLLQLLGG"
     gene            complement(169..1416)
                     /locus_tag="PLS34_11980"
     CDS             complement(169..1416)
                     /locus_tag="PLS34_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRP88216.1"
                     /translation="MSSSWPWFVGARYLRARTHNRFVSFISSISILGVALGVAVLIVV
                     LSVMNGFETELRTRILAMTSHASLESFGTGLGDWASVRDRVEQDPEVRAAAPYIEGEA
                     MLLQGELVSGAIVRGIVPAEERRVSGLADKLVAGTLADLAAGEWNIVIGSALARELQV
                     APGDRVVLAISQGTVTPAGLAPRLRRFTVAGVFEAGMYEFDRGLAFVHLEDAARLWRM
                     SGEVTGLRLALDDMFRAPEVSRRVATDLGGGFYVSDWTRQHVNFFRSIQLSKQMMFTI
                     LLLVVAVAAFNIVSTLVMVVKDKQGDIAILRTLGASPAGIMVVFMIQGTLIGIAGTLG
                     GLGLGALLALNIEQLVHWLEAAVGTTFLAADVYFISDLPAELRAGDLFRICGTALALA
                     VLSTIYPAWRAARSQPAEALRHE"
     gene            complement(1409..2656)
                     /locus_tag="PLS34_11985"
     CDS             complement(1409..2656)
                     /locus_tag="PLS34_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRP88217.1"
                     /translation="MFRPLELYIGLRYLRARRASRFVSFISLVSLLGVAVGVAALIVV
                     LSVMNGFEQELRGRLLNLTSHAVVSAPDGRLRDWRPVMARIEATPGVRGAAPFVEVQA
                     MLAHEGLLKGTLLRGIDPGLEAAVSRVEAHMVAGSLALLEPGADGLVRGRLLAARLRV
                     GIGDPLTLLVPTGSDTGARLVPRLRRFTVVGLFEVGMEEQDGMLALAHLEDAAAARGR
                     PGEVSGVRIELEDLFAAPRIARAVAAELGPGYAARDWTTENASYFRAVRIEKTMMTII
                     LSLVVAVAAFNIVATLVMVVTDKRSEIAILRTMGLSPGSVMRVFLVQGTAIGAVGTCL
                     GLLLGVPLALNVGTVMPALESLFGFSVLPAEVYYITRLPSDLRSGQVLMVAGIALGLC
                     LLSTIYPSLRAARTEPAEALRYE"
BASE COUNT          480 a         1001 c          887 g          340 t
ORIGIN      
        1 ccgccgagca gctgcagcag cgtggtcttg ccggagcccg aggcgccgac gatcgccagc
       61 cggtcaccgg gcatcacgtc gagctcgacg ccgttgagca ccgccacgac gttgccgcct
      121 tccacgaagc gcttgccgag tccacggcag ctcaggaccg gggatgtttc attcatgcct
      181 cagggcctcc gcgggctggc tgcgagcggc acgccaggcc ggatagatcg tcgacagcac
      241 ggccagggcg agcgccgtgc cgcagattct gaacaggtcg ccggcgcgca gctcggcagg
      301 caggtcgctg atgaaataca cgtcggccgc caggaaggtg gtaccgaccg ccgcttcgag
      361 ccagtgcacc agctgttcga tgttgagcgc gagcaaggcc ccgagcccga gtccgcccag
      421 cgtgccggcg atgccgatga gcgtgccctg gatcatgaac accaccatga tcccggccgg
      481 cgaggcgccc agcgtcctga ggatggcgat atcgccctgc ttgtctttca ccaccatcac
      541 cagcgtcgat acgatgttga acgccgccac cgcgacgacc agcagcagga tggtgaacat
      601 catctgcttg gacaactgga tcgagcggaa gaaattcacg tgctggcggg tccagtcgct
      661 cacgtagaag ccgccgccga ggtccgtagc cacgcgccgc gagacctccg gcgcacggaa
      721 catgtcgtcg agcgccagcc gcaggccggt cacttccccg ctcatccgcc agaggcgcgc
      781 ggcgtcctcg aggtgcacga aggccagccc acggtcgaac tcgtacatac cggcctcgaa
      841 gacccccgcg acggtgaagc gccgcagccg gggcgcgagc ccggcgggcg tgaccgtgcc
      901 ctgcgagatc gccagcacca cgcgatcgcc cggcgccacc tgcagctccc tggccagcgc
      961 gctgccgatg acgatgttcc attcccccgc cgcaaggtcg gccagcgtgc ctgccaccag
     1021 cttgtcggcc aggccggaca cgcggcgctc ctcggccggc acgatgccgc gcacgatcgc
     1081 gccgctcacc agttcgccct gcaacagcat ggcctcgccc tcgatgtagg gcgcggcggc
     1141 ccgcacctcg ggatcctgct ccacccggtc gcgcaccgac gcccagtcgc ccaggccggt
     1201 gccgaagctc tccagcgaag cgtgcgaggt catggcgagg atgcgcgtcc tgagctcggt
     1261 ctcgaagccg ttcatcaccg acagcacgac gatcagcacg gccacgccga gcgccacgcc
     1321 caggatggag atgctggaga tgaacgacac gaaacgattg tgggtgcgcg cgcgcaggta
     1381 acgcgcgccg acgaaccatg gccatgagct actcatagcg cagcgcctcg gccggctcgg
     1441 tgcgcgccgc gcgcagcgag ggatagatgg tggacaacag gcacagcccg agcgcaatgc
     1501 cggccaccat cagcacctgc ccggagcgca ggtccgacgg caggcgggtg atgtaataga
     1561 cctccgccgg caggacgctg aagccgaaca gcgactcgag cgccggcatc accgtgccga
     1621 cgttgagcgc gagcggtacg ccgagcagca gccccaggca ggtgccgacc gcgccgatcg
     1681 ccgtaccctg caccaggaac acgcgcatca cgctgcccgg cgacagcccc atggtccgca
     1741 ggatggcgat ctcgctgcgt ttgtcggtca ccaccatcac cagcgtggcg acgatattga
     1801 aggccgccac cgccaccacc agcgacagga tgatggtcat catggtcttc tcgatgcgca
     1861 cggcacgaaa atagctcgca ttctcggtgg tccagtcgcg cgccgcgtag cccggcccca
     1921 gctcggcggc gacggcacgg gcgatccgcg gggcggcgaa caggtcctcg agctcgatgc
     1981 gcacgcccga cacttcgccg ggacggccgc gtgccgcagc cgcatcttcg agatgcgcca
     2041 gcgccagcat gccgtcctgc tcctccatgc cgacctcgaa cagcccgacg accgtgaagc
     2101 gccgcaggcg cggcaccagc cgcgcgccgg tatcgctgcc cgtcggcacc agcagggtaa
     2161 gcggatcgcc gatgccgacc cgcaaccgcg ccgccagcaa ccggccgcgc accagcccgt
     2221 ccgcgcccgg ctccagcagg gcgagcgagc cggccaccat gtgcgcctcc acccgcgaga
     2281 ccgcagcctc gagcccggga tcgatgccgc gcagcagcgt gcccttgagc agtccctcgt
     2341 gcgccagcat ggcctgcacc tcgacgaagg gcgcggcgcc ccgcacaccg ggcgtcgcct
     2401 cgatgcgcgc catgaccgga cgccagtcgc gcaggcggcc gtccggtgcg ctgaccacgg
     2461 catgcgaagt gaggttcagc aggcggccgc gcagttcctg ctcgaagccg ttcatcaccg
     2521 acaacaccac gatcagcgcc gcgacgccca ccgccacgcc gagcagggac accagcgaga
     2581 tgaaggacac gaatcggctc gcccgccgcg cgcgcaggta gcgaaggccg atgtacagtt
     2641 cgaggggtcg gaacatggcg cgccattaaa gcatagggtg acgcgcgtcc ggttttttcc
     2701 ggcggcgc
//
LOCUS       DAOMPG010000288         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15196_length_2698_cov_5.037836, whole genome shotgun sequence.
ACCESSION   DAOMPG010000288 DAOMPG010000000
VERSION     DAOMPG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15196_length_2698_cov_5.037836"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..>2698)
                     /gene="plsB"
                     /locus_tag="PLS34_11990"
     CDS             complement(267..>2698)
                     /gene="plsB"
                     /locus_tag="PLS34_11990"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HRP88218.1"
                     /translation="WRLALLLTRKLLFLWVRTRKLEAQLQRLKALADQPVCFVLEHPA
                     FADLAVLEQECIRNGLPRPSAGLSFDGTREWRAVAYLRRKPGRRRKAGRLERLAEAVQ
                     AGRVEEVVIVPVSVFWGRSPDKEGSFFKVLFSEGWGLVGRLRKLFIIIFHGRNVLVQF
                     SEPVRMSQLVAEGPNPALVARKLGRVLRVHFRRLRIATIGPDLSHRRTLVNQVLNSDP
                     VRRAIRRHSEVNKVPIQKAVARARRYANEIAANYSYTTVRVMERLLAWLWNRLYDGIE
                     VSGIERLKAVTEGNEVIYVPCHRSHIDYLLLSYVVYQNGLVPPHIAAGVNLNLPVIGP
                     VLRSGGAFFMRRSFRDDPLYAAVFRRYFSLTLARGVAMEYFIEGGRSRTGRLLPAKPG
                     MLAMTVRAYLHERVRPVVFVPVYFGYERLFEGRSYVSELSGRPKQKETVLGMLKALRQ
                     LRREFGRVHVNFGAPFLLDEMLDAHRPAWRTETIEDDDKPAWSGPVVENLGDEILKRI
                     NAAAAVSPINLLATALLATPRQSMVEQPLVRILELFAAMLRVAPYGECVTVTPLGGAE
                     MIAYGERLGLLERRAHRLGDILSLAPKEALLATYFRNNTAHLLALPSLVACCFLDKPV
                     IAVERVHGLARMVYPYLGGELFLRWQPDEIDGEVDRIIDMLVAQGLLRRDESGGAPCL
                     RRPAPHTPEAIQLWVLAQCTVQTLERFYMTAALLAKHGSGTLRPGQLEDLCHLMAQRM
                     SMLFQFSAPEFFDKSLFRGFIGRLRRNGVLRTDETGLLVFDEGLVRAMEEARLFLGDR
                     LRQDVLQAMNL"
BASE COUNT          464 a          926 c          900 g          408 t
ORIGIN      
        1 cctggagggt tgcgcgctca tgaggactcc ccagtctttc tgtttctgaa ttgtgattcg
       61 cacgtgcccc gcgaccggac tccgccggcg cgtcccgcta ctcttacaga atccgtgccg
      121 ggaaaacaac atcttggttt aacagaagcg cttcttcggc cgagcgccga cattgagtgt
      181 tgcgtgcata ccaatcgtgg ctgcgagacc gggtccggaa cgggcagctg cgcgctgcaa
      241 tgcagcatgc agtcaacgcc acctgctcac aggttcatcg cctgcaggac gtcctgccgc
      301 aggcgatcac cgaggaacag ccgcgcctct tccatggccc gcaccaggcc ctcgtcgaat
      361 accagcaggc cggtctcgtc agtgcgcaac acgccgttgc ggcgcagccg gccgatgaag
      421 ccgcggaaca gcgacttgtc gaagaactcc ggcgcgctga actggaacag catggacatg
      481 cgctgcgcca tcaggtggca gagatcctcg agctggccgg gccgcagcgt ccccgagccg
      541 tgcttggcga gcagcgcagc cgtcatgtag aagcgttcca gcgtctgcac cgtgcattgc
      601 gccagtaccc acagctggat ggcttcgggc gtatgcggcg cggggcggcg caggcagggc
      661 gccccgccac tctcatcgcg gcgcaacagg ccctgcgcga cgagcatgtc gatgatgcgg
      721 tcgacttcgc cgtcgatctc atccggctgc cagcgcagga acagctctcc gccgaggtac
      781 ggatagacca tgcgcgccag gccgtgcacg cgctccacgg cgatgaccgg cttgtccagg
      841 aagcagcagg cgaccagcga ggggagcgcc agcaggtggg cggtgttgtt gcggaaatag
      901 gtggccagca gcgcctcctt gggcgccagc gacaggatgt cgcccagccg gtgcgcccgg
      961 cgctcgagca ggcccagccg ctcgccgtag gcgatcatct cggcgccgcc cagcggcgtg
     1021 acggtcacac actcgccgta aggcgccacc cggagcatcg cggcgaacaa ctcgaggatg
     1081 cgcaccaggg gctgctcgac catgctctgg cgcggcgtcg ccagcagcgc cgtggccagc
     1141 aggttgatcg ggctgaccgc agccgcggcg ttgatgcgct tgagaatctc gtcgccgagg
     1201 ttctccacca ccgggcccga ccaggccggc ttgtcgtcgt cctcgatcgt ctcggtgcgc
     1261 caggcgggcc ggtgggcgtc gagcatctcg tcgagcagga agggcgcgcc gaaattgacg
     1321 tgcacccggc cgaactcgcg gcgcaactgc cgcagggcct tcagcatgcc gagcacggtt
     1381 tccttctgct tgggcctgcc ggacagctcc gagacgtagg agcgcccttc gaagaggcgc
     1441 tcgtaaccga aatacaccgg cacgaagacc accgggcgca ctcgctcgtg gaggtacgcc
     1501 cgcacggtca tggcgagcat ccccggcttg gcgggcagca ggcgccccgt gcggctgcga
     1561 ccgccctcga tgaagtactc catcgccacg ccgcgggcga gggtcagcga gaaataccgg
     1621 cgaaacacgg ccgcatagag cgggtcgtcg cggaaactgc gccgcatgaa gaacgcgccg
     1681 ccgctgcgca ggacgggtcc gatcacaggc aggttgaggt tcactccggc ggcgatatgc
     1741 ggtggtacca ggccgttctg gtagaccacg taggacagca gcaggtaatc gatgtggctc
     1801 cggtggcaag gcacgtagat gacctcgttg ccctcggtga ccgccttgag gcgctcgatg
     1861 ccgctgacct cgatgccgtc gtagagccgg ttccacagcc aggccagcag gcgctccatc
     1921 acccgcaccg tggtgtagct gtagttggcg gcgatctcgt tggcgtagcg ccgcgccctg
     1981 gcgaccgcct tctggatcgg caccttgttg acctcgctgt ggcgccggat ggcgcgccgc
     2041 acggggtcgg aattcagcac ctggttgacg agcgtgcgac ggtgcgaaag gtcgggcccg
     2101 atggtggcga tgcgcaggcg gcgaaaatgc acgcgcagca cccgccccag cttgcgcgcc
     2161 accagcgccg gattgggccc ctcggcgacg agctgcgaca tccgtaccgg ctcgctgaac
     2221 tgcaccagca cgttgcgccc gtggaagatg atgatgaaca gcttgcgcag ccggccgacc
     2281 agcccccagc cttccgagaa aagcaccttg aaaaacgagc cttccttgtc cggcgagcgc
     2341 ccccagaaca ccgacaccgg cacgatcacc acttcctcta cgcgccccgc ctgcacggcc
     2401 tcggcaagcc gctcgaggcg cccggccttg cgcctgcggc cgggcttccg ccgcaggtag
     2461 gcgacggcgc gccattccct ggtcccgtcg aagctcagcc ccgcagacgg ccgcggcaga
     2521 ccgttgcgaa tgcattcctg ctcgagcacg gcgaggtcgg cgaaggccgg gtgctccagc
     2581 acgaagcaga ccggctggtc cgcgagcgcc ttgagtcgct gcagttgggc ttcgagcttg
     2641 cgggtgcgca cccagaggaa cagcagcttc ctcgtcagca gtaacgccag ccgccaga
//
LOCUS       DAOMPG010000289         2673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15363_length_2673_cov_2.617647, whole genome shotgun sequence.
ACCESSION   DAOMPG010000289 DAOMPG010000000
VERSION     DAOMPG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15363_length_2673_cov_2.617647"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLS34_11995"
     CDS             complement(<1..316)
                     /locus_tag="PLS34_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRP88219.1"
                     /translation="MRWACVLLPHLALDGALRAGGPDDAPFALVDGPAQRRVLHDACD
                     RARALGLKPGQPLGVAQALAGRLQVRPRDVREEARQRDLLAAWAYGFSSQVAAFGPRA
                     LAV"
     gene            complement(324..989)
                     /gene="imuA"
                     /locus_tag="PLS34_12000"
     CDS             complement(324..989)
                     /gene="imuA"
                     /locus_tag="PLS34_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HRP88220.1"
                     /translation="MGALRQVELAVAANDAASPVVLEALLDGRRLWKGRGAPARPRAD
                     AVPTGHAALDAALPGGGWPQAALSEILHPADGMGEIRLAWPALARLAQAGQRVVLVAP
                     PHLPCAPAWRSAGVEPARLQLVQATDPREALWAAEQCLRSGSCGAVLCWPHRADDRAL
                     RRLQVAAETGRSLAFAWRPLAAQRNPSPAALRLAIGPEPNALRVLKCRGGAVPARPIS
                     LAQ"
     gene            complement(991..1608)
                     /gene="lexA"
                     /locus_tag="PLS34_12005"
     CDS             complement(991..1608)
                     /gene="lexA"
                     /locus_tag="PLS34_12005"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRP88221.1"
                     /translation="MYPMDELSPRRRCILEFIRERALVHGQPPSLAEIAEACGLASRG
                     AARKQVLALADAGLIELTPGQARGIRPAGAKREAGALPVPILGRVAAGVPIGADAGLG
                     DTLSLDARLFSLRPDYLLRVEGDSMIEDGILDGDLVGVHRSAEAREGQVVVARLDGEL
                     TIKRLSLREGRVRLLPRNRAYAPIEVGPGQDFAIEGLYCGLVRRG"
     gene            1728..>2673
                     /locus_tag="PLS34_12010"
     CDS             1728..>2673
                     /locus_tag="PLS34_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP88222.1"
                     /translation="MNHAAHDPASLELSPEQMRTMGQTVVERAVAHLAALDRAPSNGD
                     FTDIEALCRALREDAPEDGAPLESLLDPLFEEWIPRSFGTPGRGYFAYIPGGGVYPAA
                     LADFIADTANRYTGIWQAAPALVQLEANTLDWLRDWMQFPETARGLYTPGGSMAMFNA
                     IAAAREDRLGHNIRAGTLYVSTQSHHCITKAARLAGVAHDRVRQVAVDGRFRMRPDAL
                     AAAIAADRAAGLTPFMVFSTAGTTNTGAVDPLDAIADLCARENLWHHVDGAYGAFFHM
                     VPALRPLLAGLSRADSLTLDPHKGLFLPYGTGALLVRDG"
BASE COUNT          359 a         1052 c          934 g          328 t
ORIGIN      
        1 ccaccgccag cgcgcgcggg ccgaaggcgg ccacctgcga gctgaagcca taggcccagg
       61 ccgccagcag gtcgcgctgg cgcgcctctt cgcgcacgtc gcgcgggcga acctgcagcc
      121 ggccggccag cgcctgcgcc acgcccagcg gctgccccgg cttcaatccc agcgcgcggg
      181 cccgatcgca ggcatcgtgc agcacgcgcc gctgcgcggg gccgtcgacc agcgcgaacg
      241 gagcatcgtc cggcccgccc gcgcgaaggg caccgtccag cgccagatgc ggcagcagca
      301 cgcaggccca gcgcacgggt gtctcactgc gcgagcgaaa tcggccgcgc cggcaccgcg
      361 ccgccgcggc acttgagcac gcgcagcgcg ttgggctcgg ggccgatggc caggcgcagc
      421 gccgccggcg aggggttgcg ctgcgccgcg agcggacgcc aggcgaaggc gaggctgcgg
      481 ccggtttccg ccgccacctg cagcctgcgc agggcgcgat cgtcggcgcg atggggccag
      541 cacagcaccg cgccgcagct gcccgagcgc aggcactgct cggcggccca cagcgcctcg
      601 cgcggatcgg tggcctgcac cagctgcagc cgcgccggct ccacgccggc gctgcgccag
      661 gccggggcgc agggaaggtg cggcggtgcc accagcacca cgcgctggcc ggcctgcgcc
      721 agccgcgcca gcgcaggcca ggccaggcgg atttcgccca tgccgtcggc cggatgcagg
      781 atttcgctca gcgccgcctg cggccagccg ccgcccggca gcgcggcgtc gagcgctgcg
      841 tggccggtgg gaacggcgtc ggcacggggg cgcgcgggcg cgccgcgtcc tttccacagc
      901 cggcgcccgt ccagcagcgc ttccagcacc acgggtgacg cggcgtcatt ggccgcgaca
      961 gcgagttcga cctgccggag cgcgcccatc tcagccgcgc ctcaccaggc cgcagtagag
     1021 accctcgatg gcgaagtcct gccctggtcc cacttcgatc ggtgcgtagg cgcggttgcg
     1081 cggcaggagg cgcacgcgcc cctcgcgcag cgacaggcgc ttgatggtca gctcgccgtc
     1141 cagccgcgcc accaccacct gtccctcgcg cgcctcggcg ctgcggtgca ccccgaccag
     1201 atcgccgtcg aggatgccgt cctcgatcat cgagtcgccc tccacccgca gcaggtagtc
     1261 cgggcgcagg gaaaacagcc gcgcgtcgag cgagagggta tcgcccagcc ccgcgtcggc
     1321 gccgatcggc acgccggccg ccacccggcc cagaatcgga accggcagcg ccccggcctc
     1381 gcgcttcgcg cctgccggcc gaatcccgcg cgcctgcccc ggcgtcagct cgatcaaacc
     1441 cgcgtcggcc agcgccagca cctgcttgcg tgctgccccg cgcgaggcca gcccgcaggc
     1501 ctcggcgatc tcggccagcg agggcggctg cccgtgcacg agggcgcgct cgcggatgaa
     1561 ttcgaggatg cagcggcggc gggggctgag ttcgtccatg ggatacattt gtactctttt
     1621 cggcgagcga acgccagaac ccccggcgca gcgggatgcc ctgtggcacg ggtcgcccgg
     1681 gtgcgccgtc gctatagttt tcgccccatc catccggagc acgaacggtg aaccacgccg
     1741 cgcacgatcc cgccagcctg gagctttccc ccgagcagat gcggaccatg ggacagaccg
     1801 tggtggagcg cgcggtggcg cacctggcgg cgctggaccg cgcgccgtcg aacggcgact
     1861 tcaccgatat cgaggcgctg tgccgtgcgc tgcgcgagga cgcgcccgaa gacggcgcgc
     1921 cgctggaatc gctgctcgat ccgctgttcg aggagtggat cccgcgctcg ttcggcaccc
     1981 ccggccgcgg ctacttcgcc tacatccccg gcggcggcgt ctacccggcg gcgctggccg
     2041 acttcattgc cgacaccgcc aaccgctaca ccggcatctg gcaggccgcc ccggcgctgg
     2101 tgcagctgga ggccaacacg ctcgactggc tgcgcgactg gatgcagttc ccggaaaccg
     2161 cgcgcgggct ctacaccccg ggcggctcga tggcgatgtt caacgccatc gccgcggcgc
     2221 gcgaggaccg cctcggccac aacatccgcg ccggcacgct gtatgtgtcc acgcagtcgc
     2281 accactgcat caccaaggcc gcgcgtctgg ccggcgtcgc gcacgaccgc gtgcgccagg
     2341 tcgcggtgga tggacgcttc cgcatgcggc cggacgcgct ggcagcggcc attgccgccg
     2401 accgcgccgc cgggctcacg cccttcatgg tgttttccac cgccggcacc accaacaccg
     2461 gcgcggtcga tccgctcgac gccatcgccg acctgtgcgc gcgcgagaac ctctggcacc
     2521 acgtcgacgg agcctacggt gccttcttcc acatggttcc cgcgctgcgc ccgctgctgg
     2581 ccggactttc gcgcgccgac tcgctcacgc tcgatccgca caagggcctg ttcctgccct
     2641 acggcaccgg cgcgctgctg gtgcgcgacg gcg
//
LOCUS       DAOMPG010000290         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15455_length_2659_cov_4.628648, whole genome shotgun sequence.
ACCESSION   DAOMPG010000290 DAOMPG010000000
VERSION     DAOMPG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_2659_cov_4.628648"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1576)
                     /locus_tag="PLS34_12015"
     CDS             complement(203..1576)
                     /locus_tag="PLS34_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRP88223.1"
                     /translation="MSMDSLRHLNQKGFARQRRAAVLEERIATLAGHIHAASYRLIAM
                     LREFDDLGGWSGWPSAAHWLSWRCGFSLGTSREKVRVAKALPGLPLIRAAFKRGELSF
                     AQVRALTRIATPANEATVARWATECTASQLERLVRLYRRHGEHAAALAQHRERSFTHW
                     EEDDGMVSFRVRLPAEQAAVVLKAIEAAKASLDGQDLDDVSAETRRAQIEEPPDHPDE
                     DVAAETRRARRADALVRVAEAYLEPDLRPRSTAEKYQVHVHLDARRAADGPVIDPDSP
                     ALAEETVRRLGCEAGLVPLLHDGEEILSVGRKTRAVPPAIRRALRLRDRGCRFPGCTH
                     HRHVDAHHIRHWADGGETKLSNLVELCGRHHRLLHEGGYGLKVTDDGALVFTRPDGAR
                     ILEVPRPLALSGHPLEVLQEAHRRLGVSAETHRCHWDGVHPDYGLAIQVLDQMARRPG
                     DSGNSQY"
     gene            1760..1996
                     /locus_tag="PLS34_12020"
     CDS             1760..1996
                     /locus_tag="PLS34_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88224.1"
                     /translation="MKTTDYFDAIRQRPDRAVITDAWITRAVRSPIRETIQKDGRIRR
                     WTQVDEMDNRYLRVILLADGETVHNAFFDRRFIP"
     gene            1993..2202
                     /locus_tag="PLS34_12025"
     CDS             1993..2202
                     /locus_tag="PLS34_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRP88225.1"
                     /translation="MKIRYFTDTDTLLMELRDVPVVETRDLDENTILDMDAQGNVCAI
                     TIEHASRRAGAPGFTYEQVSVESLD"
     gene            complement(2225..>2659)
                     /locus_tag="PLS34_12030"
     CDS             complement(2225..>2659)
                     /locus_tag="PLS34_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRP88226.1"
                     /translation="IEGTGVGLLLHGEPEELAPLLREPPAALRARLGEALAGLHVPAR
                     CLAGLAARPVPDAMWFGASCHSAEELAAAQAAGADYAFLGNVRPTASHPDRSGLGWER
                     FAAMVAELPLPVYGIGGLGPADLEEAWAAGAQGVAAIRGLWG"
BASE COUNT          460 a          936 c          833 g          430 t
ORIGIN      
        1 cgaggtcgaa agggcagtcc aactgatact ggacaatccc gggatcggca ctccgttggc
       61 gaacccgata aggcagttcg tgttgggcca gtttccctat tcgatccttt attgcattga
      121 gtccgactgt ctgttcatct tggccatcgc tcaccatcgg cgggggcccg gatactggtc
      181 ggtgcggcgc gggccataac gctcaatact gtgagttgcc gctgtcgcct ggtcttcgcg
      241 ccatctggtc cagcacctgg atcgccaacc cgtagtccgg gtgtacgcca tcccagtgac
      301 agcgatgcgt ttccgcggaa acgccgaggc gacgatgtgc ctcctgcagc acctccagcg
      361 ggtggccgct tagcgccagc ggccgcggca cctccaggat gcgcgcgccg tcgggacggg
      421 tgaacaccag cgcgccgtcg tcggtgacct tgaggccgta gccgccctcg tgcaacagcc
      481 ggtggtgccg gccgcacagc tccaccaggt tggacagctt cgtctcgccg ccgtccgccc
      541 agtggcggat gtggtgagcg tcgacgtggc ggtgatgggt gcagccgggg aagcggcagc
      601 cacgatccct gagccgcaac gcccggcgga tggccggcgg tacggcgcgc gtcttgcgcc
      661 ccaccgagag aatttcctcg ccgtcgtgca gcaacggcac caggcctgcc tcacagccca
      721 gtcggcggac agtttcctcg gcgagcgccg gcgagtccgg atcgatcacc ggcccgtcgg
      781 cggcacgtcg cgcatcgaga tgcacgtgca cctggtactt ctcggccgtc gagcgcggcc
      841 gcaaatccgg ctccaggtag gcctcagcga cccgaaccaa tgcgtccgcc cgcctggccc
      901 gccgcgtttc cgcggcaacg tcctcgtcgg gatggtccgg cggctcttcg atctgcgctc
      961 gccgcgtttc cgcggaaacg tcgtcgaggt cctggccgtc gagcgaggcc ttggccgcct
     1021 cgatggcctt caacaccacc gccgcctgct cggccggcag ccgcacccgg aaagaaacca
     1081 tgccgtcgtc ctcttcccag tgggtgaacg agcgctcgcg gtgctgcgcc agcgccgcgg
     1141 catgctcgcc gtgtcgccgg tacagccgca ccagccgctc gagctgcgag gcggtgcact
     1201 ccgtcgccca gcgcgctacc gtcgcctcgt tggccggcgt ggcgatgcgc gtcagcgccc
     1261 gcacctgtgc gaacgacagc tcgcctcgct tgaacgccgc gcggatcagc ggcagtcccg
     1321 gcaatgcctt tgccacccgc actttttctc gcgaggtgcc gagcgagaag ccgcagcgcc
     1381 agctaagcca gtgcgccgcc gagggccagc cgctccagcc gcccaggtcg tcgaactccc
     1441 gcagcatggc gatcaggcgg tagctggccg catgtatgtg gcccgcgagg gtggcaatcc
     1501 gctcctccag caccgccgcg cgacgctggc gggcgaagcc cttttggttg agatggcgca
     1561 gcgaatccat gctcatggcg gcacgctccc atatgtgtat gcatatacag cataatggca
     1621 tgtaactatc tgaaatgcaa actcttttat ctatcaaaac agtagatttg tgcagcgctt
     1681 ttctatgaaa gacccagtgc gccgaattca ccggaaatga cagctcccgg aacgccgcgc
     1741 tgctcaatac gctagcggcg tgaagaccac agactacttc gatgcgattc gacagcgtcc
     1801 agaccgagcg gtcatcacgg acgcctggat aacgagagcc gtgcgatcgc cgattcgtga
     1861 aaccatacag aaagacggac gcattcgacg atggacgcag gtagatgaga tggacaatcg
     1921 ctacctccgc gttatcctct tggctgacgg agaaacggtc cacaacgcgt ttttcgacag
     1981 gcgcttcatc ccatgaagat cagatacttc acggataccg acacgctgct gatggaactc
     2041 cgggatgttc cggtcgtgga gacgcgggac ctggatgaga acaccatcct ggacatggat
     2101 gcgcagggca acgtgtgtgc gatcacgatc gaacatgcat cacgacgagc cggcgcgccc
     2161 ggctttacct acgagcaggt gagcgtcgaa tctctggatt gacccgcgat cgtcccgcga
     2221 agcctcagcc ccacagcccc cggatcgccg cgaccccctg cgcgcccgcg gcccacgcct
     2281 cctcgaggtc ggccggcccg aggccgccga tgccgtacac cggcaacggc agctccgcga
     2341 ccatggccgc gaaccgctcc cagccgagac ccgaccggtc gggatgactg gccgtcggcc
     2401 ggacattgcc aaggaaagcg tagtccgcgc cggcggcctg cgcggctgcg agttcttccg
     2461 ccgagtggca cgacgcgccg aaccacatcg catccggcac cgggcgcgcc gccagcccgg
     2521 ccagacagcg cgccggcacg tgcaacccgg ccagcgcctc ccccaggcgc gcccgcagcg
     2581 ccgccggcgg ttcgcgcagc aggggcgcga gctcttccgg ctcaccgtgc aaaagcaagc
     2641 ccacgcccgt cccctcgat
//
LOCUS       DAOMPG010000291         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15519_length_2651_cov_3.825886, whole genome shotgun sequence.
ACCESSION   DAOMPG010000291 DAOMPG010000000
VERSION     DAOMPG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15519_length_2651_cov_3.825886"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1228)
                     /locus_tag="PLS34_12035"
     CDS             complement(80..1228)
                     /locus_tag="PLS34_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP88227.1"
                     /translation="MLRNIFEEEHEIFRDSVVRFLHAEVTPNLERWLEAGIIDRELFR
                     KAGEHGYCLMWADEAYGGMGVHDFRFEQILIEENAFHGDAGFGLSLHSRLCAPYIGQL
                     GNEEQKQRFLPGCVSGETILGIAMTEPGAGSDVAGIRTHAAEQGDHWVLNGSKTYISN
                     GINGDLFIVAARTVPDHPHGVGLFLVERGMAGFSRGRNLKKLGLKAQDTAELFFDNVK
                     VPKSNVLGDPRKGFYYLMQFLAEERLIVACGCVAAAEAALRMTLDYVRERRAFGRAIA
                     EFQNTRFKLADMRTRIDAAQAFIDRCVMDHNEGKLTAEVAAEAKLFASELEGWVTDEC
                     LQLHGGAGYMDEYPISRLYANARVSRIYAGSSEIMKEIIARSMGLDPR"
     gene            complement(1231..2007)
                     /locus_tag="PLS34_12040"
     CDS             complement(1231..2007)
                     /locus_tag="PLS34_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRP88228.1"
                     /translation="MSNTVSYERHGRVAVVTMNRPEVLNAFNRELAHDLRDAVMRAAG
                     DSDARAVVLRGAGGKFSSGADLKQGFPTDRRVEDLINTDFRPTLDMIAGMEKPVIASV
                     GGPAAGIGLSFALVCDLVVMAEDAYLLSPFAAIGLIPDGGATWLLARQLGYYRAYQLC
                     VESERIPAQRCLELGLANRVVPAARLDEESLSWAANLAERAPLALARTKLAMRAAMSM
                     SLTEAIAREAYLQTACLASEDAKEGIAAFLAKRKPEFKGK"
     gene            complement(2004..>2651)
                     /locus_tag="PLS34_12045"
     CDS             complement(2004..>2651)
                     /locus_tag="PLS34_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRP88229.1"
                     /translation="NDSRGFYTSRVFGTYVKEGVAMLAEGVKPALIENAARMAGMPVG
                     PLAVSDEVTLDLSYKIMKQTRKDLGEAYREHPADAVIVKFHDELKRLGKRYGAGFYEY
                     PEGGRKRLWAGLAEVYPPAAEQPGVEEVKKRLLNIMALETARCIEEGVVTDPADADVG
                     SIFGWGFPPWTGGTASYIDTVGMAEFLAECDRMADEFGEHYRPSEWLRGRKAMRT"
BASE COUNT          402 a          931 c          871 g          447 t
ORIGIN      
        1 cgcgcgcctc gaccgcggcg gggcccggcg ccgagacccg ccacttctat gacccgcagg
       61 ccggccagcg gccctgccat cagcgcgggt ccagccccat ggagcgggcg atgatttcct
      121 tcatgatctc gctgctgccg gcatagatcc gcgatacgcg cgcattggcg taaaggcgtg
      181 agatcgggta ttcgtccatg taaccggcgc cgccgtgcag ctgcaggcac tcgtcggtca
      241 cccagccctc cagctcgctg gcgaacagct tggcctccgc cgcgacttcg gccgtcagct
      301 tgccctcgtt gtgatccatg acgcagcggt cgatgaaggc ctgcgcggcg tcgatgcgcg
      361 tacgcatgtc cgccagcttg aagcgggtat tctggaactc cgcaatggcg cgcccgaagg
      421 cacggcgctc gcgcacgtag tcgagggtca tgcgcagcgc cgcctccgcg gcggcaacac
      481 agccgcaggc cacgatcagc cgctcctcgg cgaggaactg catcaggtaa tagaagccct
      541 tgcgcggatc gccgagcaca ttgctcttcg gcaccttgac gttgtcgaag aacagctcgg
      601 ccgtatcctg cgccttgagg ccgagcttct tgaggttgcg gccgcgactg aatcctgcca
      661 tgccgcgctc gaccaggaac aggccgacgc cgtgcggatg gtcgggcacc gtgcgcgcgg
      721 cgacgatgaa caaatcaccg ttgataccgt tggagatgta ggtcttcgag ccgttcagca
      781 cccagtggtc gccctgctcc gccgcatgcg tgcgaatgcc ggcgacgtcg ctgccggcgc
      841 cgggctcggt catggcgatg ccgaggatgg tctcgccgct gacacagccg ggcaggaagc
      901 gctgcttctg ctcctcgttg ccgagctggc cgatgtacgg cgcgcacagc cggctgtgca
      961 gcgacaaccc gaagccggcg tcgccgtgga aggcattctc ctcgatcagg atctgctcga
     1021 agcggaagtc gtgcacgccc atgccgccgt aggcctcgtc cgcccacatc aggcagtagc
     1081 catgctcgcc ggccttccga aacagctccc ggtcgatgat gcccgcttcc agccagcgct
     1141 ccaggttcgg ggtgacctcg gcatggagga acctcaccac ggagtcgcgg aagatctcgt
     1201 gctcttcctc gaaaatgttc ctcagcatgg tcacttcccc ttgaattcgg gtttgcgctt
     1261 ggcgaggaag gcggcgatgc cctccttcgc gtcttcgctg gccaggcagg ccgtctgcag
     1321 gtacgcctcg cgggcgatgg cctcggtgag gctcatgctc atggcggccc gcatggccag
     1381 cttggtccgc gccagcgcca gcggtgcccg ttccgcgagg ttggcggccc agctgaggct
     1441 ctcctcgtcc agccgcgcgg cgggcaccac gcggttggcc agcccgagct ccaggcagcg
     1501 ttgcgccgga atacgctccg actcgacgca cagctggtag gcgcggtagt agcccagctg
     1561 cctggccagc agccaggtcg cgccgccgtc cgggatcagg ccaatggccg cgaagggcga
     1621 cagcaggtag gcgtcctcgg ccatcaccac caggtcgcag accagcgcga acgacaggcc
     1681 gatgcccgcc gcggggccgc cgaccgaagc gatcaccggc ttctccatgc cggcgatcat
     1741 gtcgagcgtc gggcggaaat ccgtattgat caggtcctcg acgcggcgat cggtggggaa
     1801 gccctgcttc aggtcggccc cggagctgaa cttgccgccg gcgccgcgca gcacaacggc
     1861 gcgcgcgtcg ctgtcgccag cggcccgcat cacggcgtcg cgcaggtcat gggccagctc
     1921 gcggttgaag gcgttcagca cctcgggacg gttcatggtc accaccgcca cgcggccgtg
     1981 acgctcatag ctcacggtgt tgctcatgtg cgcatcgcct tgcggccgcg cagccactcc
     2041 gaggggcggt agtgctcgcc gaactcgtcc gccatccggt cgcactcggc gaggaattcc
     2101 gccatgccga cggtgtcgat gtaactggcc gtgccgccgg tccacggcgg aaaaccccag
     2161 ccgaagatcg agccgacgtc cgcgtcggcc gggtcggtca ccacgccttc ctcgatgcag
     2221 cgcgcggtct ccagcgccat gatgttcagc agccgctttt tgacttcctc gacgccgggc
     2281 tgctccgcgg ccggcggata gacctccgcg agccccgccc agaggcgctt cctgccgcct
     2341 tcggggtact cgtagaagcc cgcgccgtag cgcttgccga gccgtttgag ctcgtcgtgg
     2401 aacttgacga tcacggcgtc cgccgggtgc tcccgatacg cctcgccgag gtccttgcgg
     2461 gtctgcttca tgatcttgta ggagagatcg agggtgacct cgtcggacac cgccagcggc
     2521 ccgaccggca tgcccgccat ccttgcggca ttctcgatca gcgcgggctt gacgccctcg
     2581 gcgagcattg cgacgccctc cttgacgtag gtgccgaaca cccggctggt gtagaagccg
     2641 cggctgtcgt t
//
LOCUS       DAOMPG010000292         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_15989_length_2591_cov_5.218060, whole genome shotgun sequence.
ACCESSION   DAOMPG010000292 DAOMPG010000000
VERSION     DAOMPG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_2591_cov_5.218060"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PLS34_12050"
     CDS             complement(<1..106)
                     /locus_tag="PLS34_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HRP88230.1"
                     /translation="MNDSNRVVLIGGHGKVGLLAAGTLGAAGYSVDSII"
     gene            403..642
                     /locus_tag="PLS34_12055"
     CDS             403..642
                     /locus_tag="PLS34_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRP88231.1"
                     /translation="MARNTSVSLGDHFEQFIAGQVDSGRYGSASEVVRAGLRLLETTE
                     SRLEVLRNALIVGERSGRAKYSYESLVAELDSEQQ"
     gene            639..938
                     /locus_tag="PLS34_12060"
     CDS             639..938
                     /locus_tag="PLS34_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001898542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRP88232.1"
                     /translation="MRRCGLTRSAKADLKSIAGFTQSRWGVPQRNAYLKEMDQVFRSL
                     ADNPMMGSPCDEIREGDRKLLHGAHVIYYKQPTENEVLIVRILHGTMDVDSNIGA"
     gene            956..2254
                     /locus_tag="PLS34_12065"
     CDS             956..2254
                     /locus_tag="PLS34_12065"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP88233.1"
                     /translation="MDKTGQRIVIVGGGVAGLEIASTLGRRWRRGKDNPSITLVDSDS
                     AHVWKPMLHTIAAGTRDISQQQTTYLAQARDAGFAFQPGELRALDRSAREILLAAQHA
                     PDGRLLIPERRLGYDLLVLAIGSRANDFGTPGVAEHCYGIDSRRQADHFNREVRIRLL
                     QCLSQDGMLSVAIVGGGATGVELAAELVQLVEAAAGYGVPELASRISITLLEAGPRLL
                     AAFPEDISAATRKRLEAIGVKVMTGAQVSAATPDAFVLKDGSEVAASLKVWAAGVKGP
                     DFLADLDGLEATRSNQLIVGPTLQTTRDPAIYALGDCASLTPAGAERPLPPTAQVAHQ
                     QAKYLSTSLPKVLRGNAVPGFRNRDMGALVSLADYDAFGSLGQYGLFNGLTFRGRLAQ
                     LGHVMLYRSHQARIHGFWRGGLLWLVDRLNSLLRPTIRLD"
     gene            complement(2272..>2591)
                     /locus_tag="PLS34_12070"
     CDS             complement(2272..>2591)
                     /locus_tag="PLS34_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRP88234.1"
                     /translation="FINQTDLDALGDKASVIEAFFTYAQAEQRREAQELIDAENLEPA
                     AAKRYLSTSLRREFASENGTELNAILPRMSPLNPQYLSRKQGVFQKVSAFVEKFKGVG
                     GLD"
BASE COUNT          444 a          855 c          842 g          450 t
ORIGIN      
        1 ggatgatcga atcgacgctg tagcccgccg cgccgagggt gccggcggcc agcagaccga
       61 ccttgccgtg gccgccgatc aggaccacgc ggttggaatc attcattgtt ctctactcct
      121 gtgcatgggg ttcaaggcgc gtacgcgccc gagggtggtt cgttctatgc cgtgcgcagc
      181 cgggcttcca gccgggccac gctcttgccc acggccgagc gcgtcagccc gagcgaacac
      241 gttccctgat tacagccaag tcaagccggg acgcagcggg gcgagctgca tctgccatgt
      301 gggttgggtc ccgatatggc ctcggcttga acgcattgtc aggctccgat tgacatgcgt
      361 ctccaaacat accaatattt ggtacagctg caggaggatg ccatggccag gaacacaagc
      421 gtcagcttgg gtgatcattt tgagcagttc attgcaggcc aggtagacag cggccggtac
      481 ggctccgcca gcgaagttgt ccgcgccgga ctacgtttgc ttgagacgac cgaaagcagg
      541 ctggaggttc tacgcaacgc tctgattgtc ggtgaacgca gcggacgggc caaatacagc
      601 tatgagtcgc tcgttgcgga gctggactcg gaacagcagt gaggcgctgt ggcctgacca
      661 ggtcggcaaa ggccgacctg aagtcgattg cggggtttac gcagagccgc tggggcgtgc
      721 cgcagcgcaa cgcgtacttg aaggaaatgg atcaagtctt tcgctccctt gcggataacc
      781 ccatgatggg aagcccttgc gatgaaatcc gggaaggcga ccgaaagctt cttcacgggg
      841 cgcatgtgat ctattacaag cagccgactg aaaacgaagt gttgatcgtc cgcattcttc
      901 acgggacgat ggacgtggac tccaacatcg gcgcctgaca ggcacaatgg cgaccatgga
      961 caagaccggg cagcggatcg tgatcgtcgg cggcggcgtc gccgggctgg agatcgccag
     1021 cacgctcggc cgtcgctggc gtcggggcaa ggacaatccg tcgatcaccc tggtcgacag
     1081 cgactccgcc cacgtgtgga agccgatgct gcacaccatc gccgccggca cccgcgatat
     1141 ttctcagcag cagaccacct atctcgccca ggcgcgcgac gccggcttcg ccttccagcc
     1201 gggcgaactg cgcgcgctgg accgcagcgc cagggaaatc ctgctcgccg cccagcacgc
     1261 gcccgacggc cgcctgctga ttcccgaacg caggctcggc tacgacctgc tggtgctggc
     1321 catcggcagc cgcgccaacg acttcggcac gccgggcgtg gctgagcact gctacggcat
     1381 cgactcacgc cggcaggccg atcacttcaa ccgcgaggtg cgcatccgcc tgctgcagtg
     1441 cctgagccag gacggcatgc tgtcggtcgc gattgtcggt ggcggcgcca ccggcgtgga
     1501 gctggcggcc gaactggtgc aactggtcga ggccgcggcc ggttacggcg tgccggagct
     1561 ggcgtcgcgt atctcgatca ccctgctcga agccggcccg cgcctgctgg cggccttccc
     1621 ggaagacatt tccgccgcca cgcgcaagcg gctcgaagcg atcggggtca aggtgatgac
     1681 cggcgcccag gtcagcgcgg cgacacccga tgccttcgtg ctcaaggacg gcagcgaggt
     1741 ggcggcgtcg ctcaaggtct gggccgctgg cgtgaagggg ccggacttcc tcgccgacct
     1801 cgacggcctg gaggcgacgc gcagcaacca gctcatcgtc gggccgaccc tgcagaccac
     1861 ccgcgacccg gcgatctacg cactgggcga ctgcgccagc ctgacgcctg cgggcgccga
     1921 acgccccttg ccgccgaccg cccaggtggc ccaccagcag gccaagtacc tgagcacctc
     1981 actgccgaaa gtgctgcgtg gcaacgccgt gcccggcttc cgcaaccgcg acatgggcgc
     2041 actggtgtcg ctggccgact acgacgcctt cggctcgctc ggccagtacg gcctgttcaa
     2101 cggcctcacc ttccgcggcc gcctggccca actcggccac gtcatgctct accgcagcca
     2161 ccaggcgcgc atccacggct tctggcgcgg tgggttgctg tggctggtgg atcgcctcaa
     2221 ctcgctgctg cggccgacga tccggctgga ttgaggggcg cgatcggaga ctcaatccaa
     2281 cccgcccacg cccttgaact tttcgacgaa cgcggaaact ttctggaata caccctgctt
     2341 cctgctgagg tattgcgggt tgagcgggct catccgcggc aggatcgcat tgagttccgt
     2401 gccgttctcg ctggcaaatt cccgtcgcag cgaggtgctg aggtagcgct tcgccgccgc
     2461 aggttccagg ttctcggcat cgatgagttc ctgcgcttcc cgccgttgct ctgcttgggc
     2521 gtaggtgaag aacgcttcga tcacgctggc cttgtcgccc agcgcatcca ggtcggtctg
     2581 gttgatgaaa t
//
LOCUS       DAOMPG010000293         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16143_length_2571_cov_3.584261, whole genome shotgun sequence.
ACCESSION   DAOMPG010000293 DAOMPG010000000
VERSION     DAOMPG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16143_length_2571_cov_3.584261"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1567)
                     /locus_tag="PLS34_12075"
     CDS             complement(41..1567)
                     /locus_tag="PLS34_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRP88235.1"
                     /translation="MSSLLPEGSLPRVTRRRDPASLPQLDHLVVVIAANTPKSAWAGV
                     PGGAQLAAAARRRQLTKAGSVFTLHLAEPCPVTVGIAAADAAPFAWLTLARKLAAEVR
                     TSRPAKVGVMTAGAREGVAQAWLEAAISALLAAAGEMPEFRSGTPRPPSRLSHVTLLD
                     DAPQLDLERMRAEAEGNHLARWLTALPPNRLDAGSYRGLLESLAGHEGWELSFFDERA
                     LEELGAGAFLAVARANPHRNAGIARLRYRPKGRDRDAAPVALVGKGICFDTGGTNLKP
                     FRAMLDMHEDMQGSAVALGTLLALTRIGYGGAVDCWLAITENRIGPNAYNSRDVVTAA
                     NGVTIEVIHTDAEGRMALADALALAGREKPGLIIDYATLTGACVAALTHRYSGVFGNR
                     PALLETAVEAGRASGERLWPFPMDEDFDEALESRVADVLQCSVDNDGDHILAARFLQR
                     FVPKDTPWLHIDLSSGHHKGGLGLAPTAQTGFGVRLTVAMLVDHKIGDAAVAQRGGDT
                     "
     gene            1749..>2571
                     /gene="aceE"
                     /locus_tag="PLS34_12080"
     CDS             1749..>2571
                     /gene="aceE"
                     /locus_tag="PLS34_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150844.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRP88236.1"
                     /translation="MSYDPSFRDEDPDETREWLESIDSVLQAHGGERAHWLVERLIDH
                     VRRAGHYLPFSPNTAYLNTISLAREPSYPGDREMEKRIEAWLRWNAMAMVVHANRESS
                     EYGGHIASYASSLTLYEVGFHHFWRGATDDNRGDMIFIQGHSSPGIYARSFLEGRLGE
                     EDLLKFRKEVSGGLSSYPHPWLMPDYWQFPTVSMGLGPMQAIFQAKFTKYLEHRGLVE
                     PSDRKVWAFLGDGEMDEPESMGAIAMAGREKLDNLIFVINCNLQRLDGPVRGNGKI"
BASE COUNT          403 a          889 c          899 g          380 t
ORIGIN      
        1 aggtgcaggt gccagtcgtc cgggcggcgg atgcgcagct tcaagtgtcg cctccccgct
       61 gtgcgacggc cgcatcaccg atcttgtgat ccaccagcat ggcgaccgtc aggcgcaccc
      121 cgaagccggt ctgcgcggtc ggcgccaggc cgagcccgcc cttgtggtga cccgacgaca
      181 ggtcgatgtg cagccacggc gtgtccttgg ggacgaagcg ctgcaggaag cgcgcggcga
      241 ggatgtggtc gccgtcgttg tccaccgagc actgcagcac gtcggccacc cggctctcca
      301 gcgcttcgtc gaagtcctcg tccatgggga acggccagag acgctcgccg ctggcgcggc
      361 cggcctcgac ggcggtttcg agcagcgccg ggcggttgcc gaacacgccg ctgtagcgat
      421 gggtcagcgc cgccacgcag gcgccggtca gcgtggcgta gtcgatgatc agccccggct
      481 tttcgcggcc ggccagcgcc agcgcatcgg ccagcgccat gcggccctcg gcgtcggtgt
      541 ggatgacctc gatggtcacg ccgttggccg cggtgaccac gtcgcgcgag ttgtaggcgt
      601 tggggccgat gcggttctcg gtgattgcca gccagcagtc gacggcgccg ccgtagccga
      661 tgcgggtgag cgccagcagc gtgccgagtg ccacagcgct gccctgcatg tcctcgtgca
      721 tgtccagcat ggcgcggaac ggcttgagat tcgtcccgcc ggtgtcgaag cagatgccct
      781 tgccgaccag cgcgaccggc gctgcgtcgc ggtcgcggcc cttcggccgg tagcgcagcc
      841 gcgcgatgcc ggcgttgcga tgcgggttgg cgcgcgccac cgcgaggaag gcgccggcgc
      901 cgagttcctc cagcgcccgc tcgtcgaaga aggacagctc ccagccctcg tggcccgcca
      961 gcgattccag caggccgcga tagctgccgg catccagccg gttgggcggc agcgcggtga
     1021 gccagcgggc gaggtgattg ccttccgcct cggcgcgcat gcgctccagg tcgagttgcg
     1081 gtgcatcatc gagcagagtg acgtgcgaca gccgcgacgg cgggcgcggc gtgccgctgc
     1141 gaaactccgg catctcgccg gccgccgcca gcagcgccga aatcgccgcc tccagccacg
     1201 cctgtgcaac accttccctg gcccccgcgg tcatcacgcc gaccttggcc ggccggctcg
     1261 tgcgcacctc ggcggccagc ttgcgcgcca gcgtcagcca ggcgaaaggc gcggcgtcgg
     1321 ccgcggcgat ccccaccgtg accgggcagg gctcggccag gtgcagcgtg aacaccgagc
     1381 cggccttggt gagctgacgg cgacgcgcgg cggcggcgag ctgagcgcca cccggcacgc
     1441 cggcccaggc gctcttcggc gtgttcgcag cgatcaccac gaccaggtgg tccagctgcg
     1501 gcagcgaagc gggatcgcga cgccgcgtga cgcgcggcag agaaccttcc ggcagcagac
     1561 tggacatggg tgatccttga cctcaccagg gggagccacg aagataccgg agcgataaga
     1621 acacggtcgc gagtcgccca gcggcactta tgttgcaggg cgccatcgta gcagcaaggc
     1681 cgcgaatatt catccgagcg actgttggac gacttcggtc gcacccaccc cgagggagtc
     1741 ggtagcagat gtcgtacgac ccgagtttcc gcgacgagga tccggacgag acgcgcgagt
     1801 ggctggagtc gatcgactcc gtgctgcagg cgcatggcgg cgagcgcgcc cactggctgg
     1861 tggagcggct catcgaccac gtgcggcgcg ccgggcacta cctgccgttc agcccgaaca
     1921 cggcgtacct caacaccatc tcgctggcgc gcgaaccgtc ctatccgggc gatcgcgaga
     1981 tggaaaagcg catcgaggcc tggctgcgct ggaacgccat ggcgatggtg gtgcacgcca
     2041 atcgcgagag ctccgagtac ggcggccaca tcgccagcta cgcctcctcg ctgacgctct
     2101 acgaagtcgg cttccaccat ttctggcgcg gcgcgaccga cgacaaccgc ggcgacatga
     2161 tcttcatcca gggccattcg tcgcccggca tctacgcgcg ctccttcctc gagggacggc
     2221 tgggcgaaga ggacctgctc aagtttcgca aggaagtcag cggcggcttg tcgtcgtatc
     2281 cgcatccctg gctgatgccc gactactggc agttcccgac ggtgtcgatg ggcctcgggc
     2341 cgatgcaggc catcttccag gcgaagttca ccaagtacct ggagcatcgc ggcctggtcg
     2401 agccgtccga ccgcaaggtg tgggccttcc tcggcgacgg cgagatggac gagcccgagt
     2461 cgatgggcgc catcgcgatg gccgggcgcg agaagctcga caacctgatc ttcgtgatca
     2521 actgcaacct gcagcgcctc gacggcccgg tgcgcggcaa cggcaagatc a
//
LOCUS       DAOMPG010000294         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16156_length_2569_cov_4.108990, whole genome shotgun sequence.
ACCESSION   DAOMPG010000294 DAOMPG010000000
VERSION     DAOMPG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16156_length_2569_cov_4.108990"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLS34_12085"
     CDS             complement(<1..949)
                     /locus_tag="PLS34_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP88237.1"
                     /translation="MSNPLADVENFLAATPPFDLLDQGLRHRAAAAVEAVYRRKGTVL
                     LEIGEQNETLYLLRRGAVEAHDRKGHLVGRYGEGDSFGLQSLLTGKPVRFRITLIEDG
                     LVWMMPQAAFDQLRAQSTAFDTFYIRSLEERLIAALQPESGGGRSQTLFMTPMGELAR
                     RAPITVTPATSIADAAKKMSEHGISSLLVNEGDAVCGIVTDRDLRNRVLAVGRDPAEP
                     VTAIMTAEPLTLEDSSPVLAGILAMAGRGIHHLPLTRGDRVVGMVTTRDLMSLQTHHP
                     LYLAAQLQKQDSVADLAETCKRVPKLFELMLASGMRAEEV"
     gene            1044..1802
                     /locus_tag="PLS34_12090"
     CDS             1044..1802
                     /locus_tag="PLS34_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HRP88238.1"
                     /translation="MSGPDKPRDPRIVRRSPPGEGSRFSWSELLPSRLLQTLRSYFGL
                     GRAAPPEPIASPAALVRFIDTRASFIAQTSLYGYLRTRAGMRYPELFDDDPFVEGINI
                     AKWHIWLDCVGDLAVFAGSRLAHRSPHDTPRIARMMTVLLDDILAQAGEPEEAGGEFP
                     AHADRVRERIRRTDWLAVGEAEAAFSDSPPSVVKWSPVMDELKQLDEEIVLNSVRFRW
                     QEVRRAFVQDLQPRPLLAALDAYPPEPGNSSRPR"
     gene            complement(1763..2131)
                     /locus_tag="PLS34_12095"
     CDS             complement(1763..2131)
                     /locus_tag="PLS34_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88239.1"
                     /translation="MIEGIPLWVVALDYALGVVMWTLIGRFGMRIFLPEESKFFFSRF
                     FIRVTNPLLRLFKPITPEFLVPAMVPLYVAWFFFMFRFYVMPWMLGYTVMGMLSFPLE
                     GQIAQGIYYLGRLLFPGSGG"
     gene            complement(2247..>2569)
                     /locus_tag="PLS34_12100"
     CDS             complement(2247..>2569)
                     /locus_tag="PLS34_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRP88240.1"
                     /translation="SGLFFPLVLGVWWKRANRPGAIAGMLVGLFAGWWYLVAVRNPDA
                     WYGISAPWVGLNDLRFGIVGITASLIAMVVVTLLTPAPDEETQRMVDEVRIPRGDTII
                     TSKA"
BASE COUNT          430 a          891 c          861 g          387 t
ORIGIN      
        1 gcacctcttc ggcccgcatg cccgaggcga gcatcagctc gaacagcttc ggcacacgct
       61 tgcaggtctc ggccaggtcc gccacggagt cctgcttctg cagctgggcg gcgagataca
      121 gcgggtggtg agtctgcagg ctcatcaggt cgcgcgtcgt gaccatgccg accacgcggt
      181 cgccgcgcgt cagcggcagg tggtggatgc cgcggccggc catggcgaga atcccggcga
      241 gcaccgggct ggaatcctcc agcgtcaacg gctcggcggt catgatggcg gtgaccggct
      301 cggccgggtc ccggccgacc gccagcacgc gattgcgcag gtcccggtcc gtgacgatgc
      361 cgcacacggc gtcgccctcg ttgaccagca gcgaggagat gccatgctcc gacattttct
      421 tcgccgcgtc ggcgatcgag gtcgcgggcg tcaccgtgat gggtgcgcgc ctggccagct
      481 cgcccatcgg ggtcatgaac agcgtctggg accgcccgcc gccggactcg ggctggagcg
      541 cggcgatcag ccgttcctcc aggctgcgga tatagaaggt gtcgaaggcc gtggactggg
      601 cgcgcagctg gtcgaaggcg gcctgcggca tcatccagac caggccgtcc tcgatcaggg
      661 tgatgcggaa gcgcaccggc ttgccggtca gcagcgactg cagcccgaag ctgtcgccct
      721 cgccgtagcg gccgaccagg tggcccttcc tgtcgtgcgc ctccaccgcg ccacggcgca
      781 gcaggtacag cgtctcgttc tgctcgccga tctccagcag cacggtgccc ttgcgccggt
      841 acaccgcctc gacggcggcg gcggcccgat gccgcaggcc ctggtccagg aggtcgaagg
      901 gcggggttgc ggcgaggaag ttttcgacgt cggccagcgg gttgctcatg gccgggaatc
      961 atagcgtcaa acggtgcgcc aaccctatgg cgccccgtca aggccctata atgccggctg
     1021 ggtcagcaac aacggccaga gcgatgtccg gtcccgacaa gccccgtgat ccccgcatcg
     1081 tgcgccgctc gccgcccggc gagggctccc ggttttcgtg gtcggagctg cttccgtcca
     1141 ggctgctgca gaccttgcgg agctatttcg ggctggggcg cgcagcgccg cccgagccca
     1201 tcgccagccc ggcggcactg gtacggttca tcgacacccg ggccagcttc atcgcccaga
     1261 cctcgctgta cggctacctg cgcacgcgtg caggcatgcg ctacccggag ctgttcgacg
     1321 acgatccctt cgtggaaggc atcaacatcg ccaagtggca catctggctt gactgcgtcg
     1381 gtgacctggc cgtgttcgcg ggcagtcggc tcgcccaccg gtcgccgcac gacacgccgc
     1441 gcatcgcgcg gatgatgacg gtgctgctgg acgacatact ggcccaggcg ggagagcccg
     1501 aggaagcggg cggcgaattc ccggcgcacg ccgaccgggt gcgggaacgc atccggcgca
     1561 ccgactggct ggcggtcggc gaggccgagg cagccttcag cgacagcccg ccttcggtgg
     1621 tgaagtggtc gcccgtgatg gacgagctca agcagctcga cgaggaaatc gtgctcaatt
     1681 ccgtgcgttt ccgctggcag gaagtgcgcc gcgccttcgt ccaggacctg cagccgcggc
     1741 ccttgctggc cgcactcgac gcctacccgc cggagccggg gaacagcagc cggccgaggt
     1801 agtagatgcc ctgtgcgatc tggccttcca gcgggaagga cagcatgccc atgaccgtgt
     1861 agccgagcat ccacggcatg acgtagaagc ggaacatgaa gaagaaccac gccacgtaga
     1921 gcggcaccat cgccggcacc aggaactccg gcgtgatcgg cttgaacagc ctcagcagcg
     1981 gattggtcac gcggatgaag aagcggctga agaagaactt gctctcttcc ggcaggaaga
     2041 tgcgcatgcc gaagcgtccg atcagcgtcc acatcaccac gccgagcgcg taatccagcg
     2101 ccaccaccca cagcggaatt ccctcgatca tccagtcact ccaggtccgt tgcttgctcg
     2161 ccgccgcgcg cggcgcatga atcctctcac atgaaaaagg ccggggcgag tcgcctcgcc
     2221 ccggcccctg gccttgaacc gaacgatcag gccttggacg tgatgatggt gtcacccctc
     2281 gggatgcgaa cctcgtccac catccgctgg gtctcctcgt ccggcgccgg cgtcagcagc
     2341 gtcaccacca ccatggcgat gaggctggcg gtgatgccga cgatgccgaa acgcaggtcg
     2401 ttgaggccga cccagggtgc cgagatgccg taccaggcgt ccgggttgcg cacggccacc
     2461 aggtaccacc agcctgcgaa cagccccacc agcatgccgg cgatggctcc cggccggttg
     2521 gcgcgcttcc accacacgcc gagcaccagc gggaagaaca ggcccgaca
//
LOCUS       DAOMPG010000295         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16297_length_2552_cov_3.828594, whole genome shotgun sequence.
ACCESSION   DAOMPG010000295 DAOMPG010000000
VERSION     DAOMPG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_2552_cov_3.828594"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLS34_12105"
     CDS             complement(<1..963)
                     /locus_tag="PLS34_12105"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HRP88241.1"
                     /translation="MDRLLTAGFRHNSRRLTRFARPQPSESLPPPDPSRKYMLYLHVP
                     FCTVLCPFCSFHRVCFEEQKAVKYFQSLRQEIRMAHEVGYRFSDLYVGGGTPTVMPGE
                     LAGTLELLQKLSPIESISIETNPDDLRPEVMAQMRDAGVSRVSVGIQSLDDTLLKQMD
                     RYEKYGSSAEVMRRLEGAHGYFETLNADMIFNLPHQDEASLLRDIDLLTRQLEIGQVT
                     FYPLMSAESTMRKMLKTMGEVDFDREGRMYRLILDSLPDTYRPSSAWCFNRAGDTVDE
                     YIIENEEYLGLGSGSLSYLDGVLYSSTFSLNHYNRQIEAGRFGIS"
     gene            1131..2261
                     /locus_tag="PLS34_12110"
     CDS             1131..2261
                     /locus_tag="PLS34_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HRP88242.1"
                     /translation="MATSALEAGDWSEHRRYLWLASLLLPMLPLASLLAWSTWPQAWL
                     LWLTPVTVYGLVPLLDLLCGEDRNNPPESLVPALEEDRWYRWLTWLTVPAVWLTVVAT
                     AWVAVRGDLSPLGWLGLAVSAGWTSGAGINVAHELGHKKGRLERWLARFALAPACYGH
                     FVVEHVRGHHRDVATPADPASSRLGESYYRFMMREIPGACRRAWALERERLAGLGHGV
                     FHVGNENLQAWALSAGFWGVLLLALGPLVLPFLLLQAVFAYSLLSAANYVEHYGLLRA
                     RGPDGRYERVAPCHSWNSNHVVSNLLLYQLQRHSDHHAWPARRYQSLRHFEDAPQLPT
                     GYFGMFLLALVPPLWRRVMDPRVLAHAGGDLRRVNRLDAAPA"
BASE COUNT          376 a          814 c          907 g          455 t
ORIGIN      
        1 gctgatcccg aatcgccccg cctcgatctg gcggttgtaa tggttcagtg agaacgtgct
       61 cgagtacagg acgccgtcca ggtagctcag cgaaccgctg ccaagcccga ggtattcctc
      121 gttctcgatg atgtactcgt cgaccgtgtc gccggcccgg ttgaagcacc aggccgagga
      181 gggtcggtag gtgtcgggca gcgagtccag gatcagccgg tacatgcggc cctcgcggtc
      241 gaagtccacc tcgcccatgg tcttgagcat cttgcgcatg gtgctctcgg cgctcatcag
      301 cgggtagaag gtcacctggc cgatctcgag ctgccgcgtg agcaggtcga tgtccctgag
      361 cagcgaggcc tcgtcctggt gcggcaggtt gaagatcatg tcggcattga gggtctcgaa
      421 atagccgtgc gcgccctcca ggcgcctcat cacctcggcc gacgagccgt atttctcata
      481 gcggtccatc tgcttcagca aggtgtcgtc gaggctctgg atgcccaccg agacccgcga
      541 gacgccggcg tcgcgcatct gcgccatgac ttcgggccgc aggtcatcgg ggttggtctc
      601 gatggagatg ctctcgatcg ggctcagctt ctgcagcagc tcgagggtgc cggccagttc
      661 gcccggcatc accgtggggg tgccgccgcc gacgtacagg tcggagaagc gatagccgac
      721 ctcgtgcgcc atgcggattt cctggcgcag gctctggaaa tacttgacgg ccttctgctc
      781 ctcgaagcac acgcggtgga aggagcagaa cgggcacagc acggtgcaaa acggcacgtg
      841 caggtacaac atgtacttgc gcgaggggtc cgggggcggc agcgactcgc tcggctgcgg
      901 ccgcgcgaaa cgcgtcagac gccggctgtt gtgccggaat cccgcggtca gcaggcgatc
      961 catgatggtc aatttcgaag ggctccgtcc acggtcagcg ccaggcacaa gtcggcaatg
     1021 gtagcgcagt tcgacgcgcg gcccgaccgg gtttcccgca gtacgccgcg accgctcagc
     1081 tcccgtattg ctagactgag tccacggtga gggcaggggg gtgccgacgc atggcgacca
     1141 gcgcactcga ggcaggggac tggagcgaac acaggcggta cctgtggctg gcgtcgctgc
     1201 tgttgcccat gctgccgctc gccagcctgc tggcgtggag cacctggcca caggcctggc
     1261 tgctgtggct gacgccggtg accgtttacg ggctggtgcc gctgctggac ctgctgtgcg
     1321 gcgaggaccg caacaatccg cccgaatcgc tggtgcccgc cctggaggag gatcgctggt
     1381 atcgctggct gacctggctg accgtcccgg cggtgtggct gacggtcgta gccaccgcgt
     1441 gggtcgcggt gcggggcgac ctgtcgccgc tcggctggct ggggctggcg gtctcggcgg
     1501 gctggaccag cggcgccggc atcaacgtgg cgcacgaact ggggcacaag aaaggacggc
     1561 tcgagcgctg gctggcgcgg tttgccctgg cgcccgcgtg ctacgggcat ttcgtggtcg
     1621 agcacgtgcg cggccatcac cgcgacgtcg cgacgccggc ggacccggcc tcgtcgcgcc
     1681 tcggcgagag ttactaccgc ttcatgatgc gcgagatccc gggcgcctgc cgccgcgcct
     1741 gggcgctgga gcgggagcgg ctggccgggc tggggcacgg cgtattccac gtgggcaacg
     1801 aaaatctcca ggcctgggcc ctcagcgcgg ggttctgggg cgtattgctg ctggccctcg
     1861 ggccgctggt gctgccgttc ctgctgctcc aggcggtgtt tgcctacagc ctgctgtcgg
     1921 cagccaacta tgtggaacat tacggcctgc tgcgcgcgcg cggtccggac gggcgctacg
     1981 agcgggtcgc gccgtgccac agctggaact ccaatcacgt cgtctccaac ctgctgctct
     2041 accagctgca gcggcattcc gaccaccacg cctggccggc ccgccgttac cagagcctgc
     2101 gccacttcga ggacgcgccg cagctgccga ccggctactt cggcatgttc ctgctggcgc
     2161 tggtgccgcc gctgtggcgg cgggtgatgg atccgcgggt gctggcgcat gccggcggcg
     2221 acctgcgccg ggtcaaccgg ctggatgccg cgcctgcctg agggtcggcg gcgcgcccgc
     2281 acggattgcg gatgaagtcg ggcaggtcat tgaaaggcca gtgtgattct tgctcgtggc
     2341 gccgtatgac tgcgccggcc gtcaatccgc ggtttcctcg atagccggca cgtggtcggc
     2401 ggctaatttt acttatgccg agggagatct ttcccgcggc acaacttttt gctgggaggt
     2461 gatgagcatg tcgacggtac gcgagttgtc cccgaagtca ccggggaagg gctcgaggag
     2521 cgtcgcgcgg atgagcaagc ctctgctggg ca
//
LOCUS       DAOMPG010000296         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16311_length_2551_cov_3.054487, whole genome shotgun sequence.
ACCESSION   DAOMPG010000296 DAOMPG010000000
VERSION     DAOMPG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16311_length_2551_cov_3.054487"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLS34_12115"
     CDS             <1..175
                     /locus_tag="PLS34_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRP88243.1"
                     /translation="AGWGAGQLEAEMAANAWLTVPASPDIIFSVPFERRWESAAALLG
                     VSITALSSEAGHA"
     gene            178..609
                     /gene="ruvX"
                     /locus_tag="PLS34_12120"
     CDS             178..609
                     /gene="ruvX"
                     /locus_tag="PLS34_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRP88244.1"
                     /translation="MAPGGVTLLAFDYGRRRIGVAVGNTLTGDARPLTTLDATEAGPD
                     WARIGALLAEWRPARLVVGVPYNDAPDEPIAGEAQRFARRLRGRFELPVDTVDERLSS
                     AEAYDRLKDGRRAGRRGAIAKKDIDSAAAAVILQDWLDRHT"
     gene            617..1570
                     /locus_tag="PLS34_12125"
     CDS             617..1570
                     /locus_tag="PLS34_12125"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRP88245.1"
                     /translation="MASQFDARGRLRHLVTLKDLPREQLVALLDLADGFLTPPGAFPA
                     RNDRLAGRTVANLFFEPSTRTRASFELAARRLGADVLSLDVSMSSRAKGESLLDTIYT
                     LQAMQVDVFVIRDAQSGVPAFICEHVAPHVAVLNAGESHLNHPTQGLLDVLTMRRHKG
                     DLSDLVVTIAGDIQHSRVARSAHHALTTLGIRELRLVAPPALAPDPAEFQGARLLDNI
                     EAGVRGADVVMCLRIQRERMEVSRNPDPEDFHREFGLTPERLALAKPDAIVMHPGPMN
                     RDVEIAGSVADGTQSVIAEQVTCGVAVRMAVMQTVMDTIHG"
     gene            1563..2438
                     /locus_tag="PLS34_12130"
     CDS             1563..2438
                     /locus_tag="PLS34_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRP88246.1"
                     /translation="MDDKPHRGTIFVEDAEVLDVEPWPGEQYIMRLVAPKTAAGARPG
                     SFAHLSCAPSLPMRRPLSIMRADPGSGVVEFLFKVVGEGLALLAQRRPGERVSVLGPI
                     GVPFAPSPGRRRPLLIGGGVGIPPMVFLAEALAERGGAPLVLMGSEIPFPFAATDSRL
                     PVPGLPAGVTAAMPLMENLGVPSRLASGAGFAGCYRGFVTELAREWLRALDEAARREV
                     EIFACGPTPMLRAAAALAQDFGLPCQVSLEEFMACAVGGCAGCAVRINTSTGPAMKRV
                     CVDGPVFDAATVDWA"
BASE COUNT          327 a          939 c          918 g          367 t
ORIGIN      
        1 cgccggctgg ggcgcgggcc agctcgaggc cgagatggcc gccaacgcct ggctcaccgt
       61 gccggcctcg cccgacatca tcttctcggt acccttcgag cgccgctggg aatccgcggc
      121 ggcgctgctc ggcgtcagca tcacggcgct gtcctccgag gcgggccacg cctgagcatg
      181 gcgcccggcg gggtcaccct gctggccttc gattacgggc ggcggcgcat cggcgtggcg
      241 gtcggcaaca ccctcaccgg cgacgcccgg ccgctcacca ccctggacgc caccgaggcc
      301 gggccggact gggcccgcat cggcgcgctg ctggccgagt ggcggccggc ccggctggtg
      361 gtcggggtcc cgtataatga cgcgccggac gagccgatcg ccggcgaggc gcagcgcttc
      421 gcgcgccgcc tgcggggccg cttcgagttg ccggtggaca cggtggacga gcggctgagc
      481 tcggccgagg cgtacgaccg gctcaaggac ggccggcgcg cggggcgccg cggggcgatc
      541 gcgaaaaagg acatcgacag cgcggccgcg gccgtgatcc tgcaagactg gctggacaga
      601 cacacctgag gactccatgg cttcccagtt cgacgcacgc ggccgcctcc gccacctggt
      661 gaccctgaag gacctgccgc gcgagcagct ggtcgcgctg ctcgacctgg ccgatggctt
      721 cctcacgccg cccggcgctt tcccggcacg caacgaccgg ctcgccgggc gcaccgtggc
      781 caacctgttc ttcgagccca gcacccgcac gcgcgcctcc ttcgagctgg cggcgcggcg
      841 gctcggcgcc gacgtgctca gcctcgacgt cagcatgtcc tcgcgcgcca agggcgaatc
      901 gctgctcgac accatctaca ccctgcaggc catgcaggtg gacgtgttcg tcatccgcga
      961 tgcgcagtcc ggcgtgccgg ccttcatctg cgagcacgtc gcgccgcacg tcgcggtgct
     1021 caacgccggc gagtcgcacc tgaatcaccc tacccagggc ctgctcgacg tgttgaccat
     1081 gcggcgtcac aagggcgacc tgtccgacct cgtggtcacc atcgccggcg acatccagca
     1141 ctcgcgcgtg gcgcgctccg cccaccatgc gctgaccacg ctcggcatcc gcgagctccg
     1201 gctggtcgca ccgccggcgc tggcgccgga cccggccgaa ttccagggcg cgcggctgct
     1261 cgacaacatc gaggccggcg tgcgcggcgc ggacgtggtg atgtgcctgc gcatccagcg
     1321 cgagcgcatg gaagtgagcc gcaaccccga cccggaggat ttccaccgcg agttcggcct
     1381 cacgcccgag cgcctggcgc tggccaagcc ggacgccatc gtgatgcacc cggggccgat
     1441 gaaccgcgac gtggaaatcg ccggcagcgt ggcggacggt acgcagtcgg tgatcgccga
     1501 gcaggtcacc tgcggcgtgg ccgtgcgcat ggccgtgatg cagaccgtga tggacacgat
     1561 ccatggatga caagccccac cgcggcacca ttttcgtcga ggacgccgag gtgctcgacg
     1621 tcgagccctg gcccggcgag cagtacatca tgcggctggt cgcgccgaag acggccgccg
     1681 gcgcacgccc ggggagcttc gcgcacctca gctgcgcgcc ctcgctgccg atgcgccggc
     1741 cgctgtccat catgcgcgcc gatcccggct cgggcgtcgt cgagttcctg ttcaaggtcg
     1801 tcggcgaggg cctggcgctg ctggcgcaac ggcggcccgg cgagcgcgtc agcgtgctcg
     1861 gcccgatcgg cgtgcccttc gccccctcgc ccgggcgccg ccggccgctg ctgatcggcg
     1921 gcggcgtggg catcccgccg atggtgttcc tggccgaggc gctggcggag cgcggcggcg
     1981 ccccgctcgt gttgatgggg tcggagatac cttttccctt cgccgcgacg gattcgcgcc
     2041 tgccggtgcc cgggttaccg gccggcgtca ccgcggcgat gccgctgatg gagaacctcg
     2101 gcgtgccgag ccggctggcc agcggcgccg gtttcgccgg ctgctaccgg ggcttcgtca
     2161 ccgagctggc gcgcgagtgg ctgcgcgcgc tcgacgaggc cgcccgccgc gaggtggaga
     2221 tctttgcctg cgggccgacg ccgatgctgc gggcggcggc ggcgctggcg caggacttcg
     2281 gcctgccctg ccaggtctcg ctggaagaat tcatggcctg tgcggtcggc ggctgcgccg
     2341 gctgcgcggt gcgcatcaac acgtccaccg gcccggccat gaagcgggtg tgcgtggacg
     2401 gcccggtgtt cgacgccgcc acggtggact gggcctgacc ggatcgcggg cggaacgcgc
     2461 cgcgctcatc tgccgttcag gttcgcttcc atagactctg ttcccgagag caatgccggg
     2521 tcattgcgga ggaacggaca tgcctgcaca c
//
LOCUS       DAOMPG010000297         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16317_length_2550_cov_4.780361, whole genome shotgun sequence.
ACCESSION   DAOMPG010000297 DAOMPG010000000
VERSION     DAOMPG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16317_length_2550_cov_4.780361"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="lptC"
                     /locus_tag="PLS34_12135"
     CDS             complement(<1..418)
                     /gene="lptC"
                     /locus_tag="PLS34_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRP88247.1"
                     /translation="MASGTRLALLALAALAAGSWWLNQRLEPGGGDVEERPAPDGFYM
                     TEAEITTAGPDGLPRYRVIADEIRQRALGGPTELHDVRVEYNIYSPSPWLMTAPQGLL
                     SDDQSLLELWGGVEVIGESGDHGRAAVETERLEIDTA"
     gene            complement(421..909)
                     /locus_tag="PLS34_12140"
     CDS             complement(421..909)
                     /locus_tag="PLS34_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRP88248.1"
                     /translation="MARLRDIAAGVRLAVFDVDGVFTDGRIWMGTDGVEYKAFSVRDG
                     VGVKQLLAAGIEVAIISGRASSAVDRRMAELGVTRVVQGCADKAEALAALLRDTGIAP
                     ADTAYLGDDTPDLPALRAVGLPAAVADAHPEVLAAAAWTTSLPGGRGAVREFCDFLLG
                     AR"
     gene            complement(909..1898)
                     /locus_tag="PLS34_12145"
     CDS             complement(909..1898)
                     /locus_tag="PLS34_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRP88249.1"
                     /translation="MDRDTTLDGNGVTQLGREVLDIEAAAVLALRDRIGEAFVHACRL
                     CLACRGRVVVTGMGKSGHVAGKIAATLASTGTPSFFVHPGEASHGDLGMITATDVVLA
                     ISNSGETPEILTILPLIKRLGVPLIAMTGRPESTLATAADAHLDVGVDKEACPLNLAP
                     TASTTATLAMGDAMAVALLNSRGFTREDFAFSHPGGQLGRRLLLHVRDVMRTGDRVPK
                     VSPDLAVSEGLFEISRKGLGMTAVVDAQDRVLGIYTDGDLRRTLDARRDLTATPIHEV
                     MTRNVKTVRGGMLAAEAVRLMETHAITALLVVDGDGCLIGAFNVHDLLQAGVM"
     gene            complement(1904..>2550)
                     /locus_tag="PLS34_12150"
     CDS             complement(1904..>2550)
                     /locus_tag="PLS34_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="YrbG; inner membrane protein involved in cell
                     envelope integrity; putative sodium ion/calcium ion
                     exchanger; in E. coli it is non essential for cell
                     viability; member of the YRBG family of cation/Ca2+
                     exchangers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRP88250.1"
                     /translation="PALLAVTLIVTMIFINQKLGRLEGILLIGTLVIVLYWLVVLGSR
                     SGDNDPIALDYASEIPEGIPMRQALGWLAIGLVVLLTGSNLLVWGAQNLAVAFGVSEL
                     VIGLTVVAIGTSLPELAVSIVSAFKGEHDMAIGNIIGSNLFNSLAVIGVAGLIHPAVF
                     ELELLTAHLPVMIGLTLALFLMTYNFGGQSRIGRVEGAILLGAFLCYHTWLVAR"
BASE COUNT          401 a          894 c          891 g          364 t
ORIGIN      
        1 tggcggtgtc gatctcgagg cgctcggttt cgaccgcggc gcggccgtgg tcgccggatt
       61 cgccgatcac ctcgacgccg ccccacaatt ccagcagcga ctggtcgtcc gacagcagcc
      121 cctgcggcgc cgtcatgagc caggggctgg gcgaatagat gttgtactcg acgcgcacgt
      181 catggagttc cgtcggcccg ccgagcgcac gctggcggat ttcgtcggcg atgacgcgat
      241 agcgcggcag gccgtccggg cccgcggtcg tgatctccgc ctcggtcatg taaaagccgt
      301 ccggagcggg gcgctcttcg acatcgccgc cgccgggctc gagccgctgg ttcagccacc
      361 agctgccggc ggccagggcc gccagcgcga gcagggccag tcgcgtgccg gaagccacgc
      421 tcagcgggcc ccgagcagga agtcgcagaa ttcgcgcacc gcgccgcgcc cgcccggcag
      481 cgaggtggtc caggccgccg ccgccagcac ctcggggtgg gcgtcggcga ccgcggcggg
      541 caggcccacc gcccgcagtg cagggaggtc cggcgtgtcg tcgccgaggt aggcggtgtc
      601 tgccggcgcg atgccggtgt cgcgcagcag cgccgcaagg gcttcggcct tgtcggcgca
      661 gccctgcacc acgcgcgtca ccccgagctc cgccatgcgg cggtccacgg ccgaggaggc
      721 acggccggaa atgatggcga cctcgatgcc ggccgccagc agctgcttga cgccgacgcc
      781 gtcgcgcacg ctgaacgcct tgtactcgac gccgtcggtg cccatccaga tcctgccgtc
      841 ggtgaacacg ccgtcgacgt cgaacacagc gagccgcacg ccggcggcga tgtcgcgcaa
      901 ccgggccatc acattacgcc cgcctgcaac aggtcgtgga cattgaaggc gccgatcaga
      961 cagccgtcgc cgtccaccac gagcagcgcc gtgatggcgt gggtctccat caggcgcacg
     1021 gcctccgcgg cgagcatgcc gccacgcacg gtcttgacgt tgcgcgtcat gacctcgtgg
     1081 atcggcgttg cggtcaggtc acggcgcgca tcgagcgtgc gccgcaggtc gccgtcggtg
     1141 tagatgccga gcacccggtc ctgcgcgtcc accaccgcag tcatgcccag tcccttgcgg
     1201 gagatctcga acagcccttc cgacacggcg aggtccgggc tcaccttggg cacccggtcg
     1261 ccggtgcgca tcacgtcccg cacgtgcagc agcaggcggc ggccgagctg cccgccggga
     1321 tgcgaaaagg cgaaatcctc gcgggtgaag ccgcggctgt tgagcagcgc caccgccatg
     1381 gcgtccccca tggcgagcgt cgccgtggtg ctggccgtcg gcgccaggtt cagcggacag
     1441 gcctccttgt cgacgccgac gtcgagatgc gcgtccgcgg cggtggccag cgtggactcc
     1501 gggcggccgg tcatggcgat caacggcacg cccaggcgct tgatcaacgg caggatggtg
     1561 agaatctccg gcgtttcgcc agagttcgag atggccagca ccacgtcggt ggcggtgatc
     1621 atgccgagat ccccgtgact ggcctcgccg gggtggacga agaacgacgg cgtccccgtg
     1681 ctggcgagcg tggcggcgat cttgcccgcc acgtggccgg acttgcccat gccggtgacc
     1741 accacgcggc cgcggcacgc caggcagagg cggcaggcgt ggacgaaggc ttcgccaatg
     1801 cggtcccgca gcgccagcac ggccgccgcc tcgatgtcga ggacctcgcg cccgagctgc
     1861 gtgacgccgt tgccgtcgag tgtcgtgtcc cggtccatga gcttcacctc gccaccagcc
     1921 acgtgtggta gcacaggaac gcgcccagca ggatggcgcc ctcgacgcgt ccgatgcgac
     1981 tttgtccccc gaaattgtat gtcatcagga acagggcgag cgtcagcccg atcatgaccg
     2041 gcaggtgggc ggtcaacagc tcgagttcga agaccgccgg gtggatcagg ccggcgacgc
     2101 cgatgaccgc caggctgttg aacaggttcg agccgatgat gttgccgatg gccatgtcgt
     2161 gctcgccctt gaaggcgctt acgatcgaca cggccagctc gggcaggctg gtgccgatgg
     2221 ccactaccgt caggccgatg acgagttccg acacgccgaa cgcgaccgcc aggttctgcg
     2281 cgccccagac cagcaggttg gagccggtca gcagcacgac gagtccgatg gccagccagc
     2341 cgagcgcctg gcgcatgggg atgccctcgg gaatctccga ggcatagtcg agcgcgatcg
     2401 ggtcgttgtc ccccgagcgg ctgccgagca ccaccagcca gtacagcacg atgacgagag
     2461 tgccgatcag caggatgccc tcgaggcgtc ccagcttctg gttgatgaaa atcatggtca
     2521 cgatcagggt caccgccagc agcgccggca
//
LOCUS       DAOMPG010000298         2548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16333_length_2548_cov_3.986763, whole genome shotgun sequence.
ACCESSION   DAOMPG010000298 DAOMPG010000000
VERSION     DAOMPG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16333_length_2548_cov_3.986763"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PLS34_12155"
     CDS             <1..1625
                     /locus_tag="PLS34_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP88251.1"
                     /translation="SDGATLVSIFEDSAKRFADRPAFACMGRTMGYAELDRLSAEFAG
                     WLSKDLGLARGARVAIMLPNLLQYPVALFGALRAGMTVVNVNPLYTPRELKHQLVDSG
                     AEAILILENFAATLEKVIAETPVKHVAVTGIGDLLPFPKRHIVNFVVRRVKKMVPQYQ
                     LSGHLRLEDALARGRQKGFEPVTVSQDDLAFLQYTGGTTGVSKGAMLTHRNMVANLRQ
                     MRAWVGGLFEEGTEVVITALPLYHVFSLVCNCLLMTSVGGLNVLITNPRDIPAFVKEL
                     STLRFTMITGVNTLFNALLQDPGFAKLDFGPLKWAGAGGMALQAAVAKRWHEVTGKYI
                     QEGYGLTETSPVVSMQPPSVTEVFTGTIGLPLPSTEVVIRDDDGRELPVGQPGELCVR
                     GPQVMKGYWQRPEATEGVMHPDGFLRTGDIATMDPQGFFRIVDRKKDMILVSGFNVFP
                     NEVENVVTQHPDVVEAACIGVPDPKSTEAVKIFVVKQPGSGLDAAGVTAWCRENLTAY
                     KVPRHVEFRDELPKTNVGKILRRALRDEATGA"
     gene            1618..2385
                     /locus_tag="PLS34_12160"
     CDS             1618..2385
                     /locus_tag="PLS34_12160"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRP88252.1"
                     /translation="MPDLPASNASWPAALLALLLCAAPSSAVATDAAERSADPAPALA
                     VPLHEAIYHATVRRIKVRAGLRLEQQDDGLYTYRSWVEPRGLLSFINRELKETSLVML
                     DGDGGIVPLSYRRRDELNGRHSDIRFDLAEHQVHIDYRGRQTTTDWEPGIYDLLSLRL
                     VLSNDLARGKLQDEYRIIDDRARVEEVDVEIAGREILSTPLGEVETLRLEYRSRRRDR
                     LFRLWIAPDMDGALIRLEQYEEGSLRGALEIVEYRRL"
BASE COUNT          402 a          877 c          887 g          382 t
ORIGIN      
        1 tcagcgacgg ggcgacgctg gtcagcattt tcgaggacag cgcgaaacgc ttcgcggacc
       61 ggccggcgtt cgcctgcatg ggccgcacca tgggctacgc ggaattggat cggctgtcgg
      121 cggaattcgc cggctggttg agcaaggacc tggggctggc gcgcggcgcc cgcgtggcga
      181 tcatgttgcc gaacctgctg cagtacccgg tggcgctgtt cggcgccctg cgggccggca
      241 tgacggtggt gaacgtcaac cccctgtaca ccccgcggga gctgaagcac cagctggtgg
      301 actcgggcgc cgaggccatc ctgatcctgg agaatttcgc cgccacgctc gagaaggtca
      361 tcgccgagac tccggtgaag cacgtcgccg tcaccgggat cggcgacctg ttgcctttcc
      421 cgaagcgcca catcgtcaac ttcgtcgtgc gccgggtgaa gaaaatggtg ccgcaatacc
      481 agctgtccgg gcacctcagg ctcgaggatg cgctggcgcg cgggcggcag aagggcttcg
      541 agccggtgac cgtcagccag gacgacctgg ccttcctgca gtacaccggc ggcaccaccg
      601 gggtgtcgaa gggcgccatg ctcacccacc gcaacatggt ggcgaacctg cgccagatgc
      661 gcgcctgggt cggcgggctg ttcgaggagg gcaccgaggt ggtcatcacc gcgctgccgc
      721 tctaccacgt gttcagcctg gtgtgtaact gcctgctgat gacctccgtg ggcggcctca
      781 acgtgctgat caccaatccg cgcgacatcc cggccttcgt caaggagttg tcgacgctgc
      841 ggttcacgat gatcacgggc gtcaacacgc tgttcaacgc cttgttgcag gatcccggct
      901 tcgccaagct cgacttcggg ccgctcaagt gggcgggcgc cggcggcatg gcgctgcagg
      961 ccgcggtcgc gaagcgctgg cacgaggtga cggggaagta catccaggag ggctacggcc
     1021 tgaccgagac ctcgccggtg gtgagcatgc agccgccgag cgtcacggag gtgttcaccg
     1081 gcaccatcgg cctgccgctg ccgtccaccg aggtggtgat ccgcgacgac gacgggcgcg
     1141 agttgccggt gggccagccg ggcgagctct gcgtgcgcgg cccgcaggtc atgaagggct
     1201 actggcagcg ccccgaggcg accgagggcg tgatgcaccc ggacggcttc ctgcgcaccg
     1261 gcgacatcgc caccatggac ccgcagggct tcttccgcat cgtggaccgc aagaaagaca
     1321 tgatcctggt gtccggcttc aacgtgttcc ccaacgaggt ggagaacgtc gtcacccagc
     1381 acccggacgt cgtcgaggcg gcctgcatcg gcgtgcccga cccgaagtcc accgaggcgg
     1441 tgaagatttt cgtcgtcaag cagccgggct ccgggctgga tgctgccggc gtgacggcct
     1501 ggtgccggga gaacctgacg gcctacaagg tgccgcggca cgtggagttc cgcgacgagc
     1561 tgccgaagac caacgtcggc aagattctcc gccgggcgct gcgcgacgag gccaccggtg
     1621 cctgacctgc ctgccagcaa cgcatcctgg ccggcggcgc tgctggcgct gctgctgtgc
     1681 gcggcgccgt cgtcagccgt tgcgaccgac gccgcggagc ggtcggccga tccggcgccc
     1741 gcgctggcgg tgccgctgca cgaggcgatc tatcacgcga ccgtgcgccg catcaaggtg
     1801 cgcgccggcc tgcgcctgga gcagcaggac gacggcctgt acacctaccg ctcctgggtc
     1861 gagccgcgcg gcctgctcag cttcatcaat cgcgagctca aggaaaccag cctggtgatg
     1921 ctggacggcg acggcggcat cgtgcccctg tcgtatcgcc ggcgggacga actcaacggc
     1981 cgccacagcg acatccgctt cgacctcgcc gagcaccagg tccacatcga ctaccgcggc
     2041 cgccagacca ccacggactg ggagccgggc atctacgacc tgctcagcct gcggctggtg
     2101 ctgtccaacg acctggcgcg cggcaagttg caggacgagt accggatcat cgacgatcgt
     2161 gcgcgggtcg aagaagtcga tgtcgagatc gcgggccgcg aaatcctgtc gacgcccctg
     2221 ggggaggtcg aaacgctgcg cctggagtac cgcagccggc gacgcgaccg gctgttccgg
     2281 ctctggatcg cgcccgacat ggacggcgcg ctgatccggc tcgagcagta cgaggagggc
     2341 agcctgcgcg gcgccctcga aatcgtcgaa taccggcggc tctaggcgcg cgccccgttc
     2401 agccgagccg cgcgcggatg cggcgatagg cctcgccgag ctcgtcggcc agcacgccga
     2461 acgccgcgcc gacgtcgcgc gacgcttcgc gggcttcccc ggcggcgcct tccatgcgcc
     2521 cctcgagccg ctcccacttg cgctccag
//
LOCUS       DAOMPG010000299         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16342_length_2547_cov_4.375201, whole genome shotgun sequence.
ACCESSION   DAOMPG010000299 DAOMPG010000000
VERSION     DAOMPG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16342_length_2547_cov_4.375201"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..612
                     /locus_tag="PLS34_12165"
     CDS             250..612
                     /locus_tag="PLS34_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRP88253.1"
                     /translation="MAYIEGFLVPIPNKNLAAYRRMARKAGKVWMEYGALQYVECIED
                     DVQPGKLTSFPQAVKLKRGEVVAFSYILYRSRRHRDSVNRKVMADPRIEAMMPDKTRM
                     PFDAARMMFGGFKSIVAF"
     assembly_gap    890..1002
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1201..1605
                     /locus_tag="PLS34_12170"
     CDS             1201..1605
                     /locus_tag="PLS34_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRP88254.1"
                     /translation="MPNTYAGGCHCGAVRFEADLDLGLGTSRCNCSICVRGRFWKAIA
                     FGDAFRLLRGAGALTRYTFGSGTIQHHFCSHCGVKPFGRGEIAELGGVFHAVNVACLD
                     DLEPDALDALPVEFQDGRADRQDRAPGFTSYL"
     gene            1634..2227
                     /locus_tag="PLS34_12175"
     CDS             1634..2227
                     /locus_tag="PLS34_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRP88255.1"
                     /translation="MKRHLPFALAAALTASAAATAAEVEYEIDPAHTFPSFEADHLAG
                     LSVWRGKFNASHGTVVLDKAAGSGRVDITIDTDSIDYGLDAMNEHARGADLFDTARWP
                     QATYTGALAGFADGKPTRVDGTLTLRGVSRPVVLEIRSFKCMPHPLHQRELCGADALA
                     TIQRDDFGMTAGKDYGFDMGVTLRIQVEAVAAEPAAK"
     gene            complement(2287..>2547)
                     /locus_tag="PLS34_12180"
     CDS             complement(2287..>2547)
                     /locus_tag="PLS34_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HRP88256.1"
                     /translation="PVVELNRAVAVSMRSGPEAGLALVDALVDAPALRAYHLLPAVRG
                     DLLHKLGRHDEARVEFERAAGMTRNERERALLLARAGSMVPD"
BASE COUNT          395 a          866 c          789 g          384 t
ORIGIN      
        1 cgtgtaccgc atcgcgctcg gcaccgccct gctgctgtgg ctgccggcgg gggactgaag
       61 caaaaccggg gtcagagtcc acttttcccg gaccctgccc tcgttggccg ggcgttgcgg
      121 gagaaaagtg gactctgacc ccggttttga ccccggttct gcacatcgcc tgtcgatcca
      181 gggcgccatc atccgtcgta ccccggacac cgcggcaccc gccgcggacc ccgacgacgg
      241 agcgatgaca tggcctacat cgaaggtttc ctggtcccga tcccgaacaa gaacctcgcc
      301 gcctaccggc gcatggcccg caaggccggc aaggtctgga tggagtacgg cgcgctgcag
      361 tacgtcgagt gcatcgagga cgacgtgcag cctggcaagc tgacctcgtt cccgcaggcg
      421 gtgaagctga agcggggcga ggtcgtggcc ttctcgtaca tcctctaccg ctcgcgtcgc
      481 catcgcgaca gcgtcaacag gaaggtcatg gccgatccgc gcatcgaagc gatgatgccg
      541 gacaagacca ggatgccgtt cgacgccgct cgcatgatgt tcggcggctt caagtcaatc
      601 gtcgcgttct gatccctcca acgggcccgc gcccggtctt gtcgatctcc cgcgccatcg
      661 ttcgtcgatg ccatggcgac ggcgatcgtc gcggcactga ccttttccac cacccacagg
      721 agccccggga atgagcagca tcaacagcgt tggctggttc gaactctacg tgcaggacat
      781 ggtgcgggcg cgcgccttct acgaagcggt cttcggcatc gcattggagg cgctgaagct
      841 gcccgagggc agcgacgacg gcctcgagat gtggtccttc cccggcgagn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgcatcg cccccgccgg
     1021 cggccgcgtg caccgcgaca agatgtcgat cggcgaatac ggcttcatcg tgctggcctt
     1081 cgacagcgag ggcaacatga tcggattgca ttcgatgcgc tagcggcgat cgaccgtcgc
     1141 aggcgcgaaa gcgacggtcg accccggccc gtcccaatcc cactgacaga gagaattccc
     1201 atgcccaaca cctacgccgg cggctgccat tgcggcgccg tccgcttcga ggcggatctc
     1261 gacctcggcc tcggcaccag ccgctgcaac tgctcgatct gcgtgcgcgg ccgcttctgg
     1321 aaagcgatcg cgttcggcga tgccttccgg ctgttgcgcg gtgctggagc gctgacgcgg
     1381 tacaccttcg gcagcggcac gatccagcac catttctgca gccactgcgg cgtcaagccg
     1441 ttcggtcgcg gcgagatcgc ggaactcggc ggcgtgttcc acgcggtcaa cgtggcctgc
     1501 ctggacgatc tcgaaccgga cgcgctcgac gcactgccgg tcgagttcca ggacggccgc
     1561 gctgaccggc aggaccgcgc gcccggcttc acttcctatc tttgaacccg ttttccggca
     1621 gaggacacct ttgatgaagc gacaccttcc gttcgcgctc gccgctgcac tcaccgccag
     1681 tgcagcggcg acggccgccg aggtcgagta cgagatcgat cccgcccaca cctttcccag
     1741 cttcgaggcc gaccacctgg ccgggctgtc ggtgtggcgc ggcaagttca atgccagcca
     1801 cggcaccgtg gtgctggaca aggccgccgg cagcggacgg gtggacatca ccatcgacac
     1861 cgacagcatc gactacggcc tcgatgcaat gaacgagcac gcgcgcggcg cggatctgtt
     1921 cgataccgcg cgctggccgc aggcgaccta caccggcgcg ctcgccggct tcgccgacgg
     1981 caagcccacg cgcgtggacg gcaccctcac cctgcgcggc gtcagccggc cagtcgtgct
     2041 cgagatccgc agcttcaagt gcatgccgca cccgcttcac cagcgcgaac tgtgcggcgc
     2101 cgatgccctg gccacgatcc agcgtgacga cttcggcatg accgccggca aggattacgg
     2161 cttcgacatg ggcgtcaccc tgcgcatcca ggtggaggcg gtggcggccg agccggcggc
     2221 gaagtgaatg cgtcatcgcg cgcggcccac gctcgctgac cttctcggcg cgggtccagg
     2281 cgcacctcag tccggaacca tgctcccggc gcgcgccagc agcagcgcgc gctcgcgctc
     2341 gttgcgggtc atgcccgcgg cgcgttcgaa ctcgacgcgc gcctcgtcgt ggcggccgag
     2401 cttgtgcagc agatcgccgc gcaccgccgg cagcaggtgg taggcgcgca gcgcaggcgc
     2461 atccaccagc gcatcgacca gggccagccc cgcctcgggt ccggagcgca tcgagaccgc
     2521 gacggcgcgg ttgagttcga ccaccgg
//
LOCUS       DAOMPG010000300         2522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16555_length_2522_cov_4.648966, whole genome shotgun sequence.
ACCESSION   DAOMPG010000300 DAOMPG010000000
VERSION     DAOMPG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16555_length_2522_cov_4.648966"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLS34_12185"
     CDS             <1..507
                     /locus_tag="PLS34_12185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931998.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRP88257.1"
                     /translation="IAWDLRKKQPYEVYAEMDFDIPVGVNGDCYDRYLVRVEELRQSN
                     RIIRQCVRWLRENPGPVSIDDHKIVPPRRVEMKDDMESLIHHFKLYTEGYCVPEGEVY
                     AAVEGPKGEFGCYLVSDGANKPYRVKLRAPGFVHLAALDEMVRGHMLADVVAMIGTID
                     VVFGEVDR"
     gene            504..1001
                     /gene="nuoE"
                     /locus_tag="PLS34_12190"
     CDS             504..1001
                     /gene="nuoE"
                     /locus_tag="PLS34_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019590690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRP88258.1"
                     /translation="MSTVKPAPLSAHVREEIDAWVKRFPAGRQRSAVLAALRAVQHEN
                     DGYLTTELMDGVADYLDLPPIQVYEVATFYSMFETKPCGRHHVSICTNISCMLRGGEE
                     LLRHAERKLGIRAGESTPDGRIFLKNEEECLAACCGAPMMMVDHVYHEDLTAAKLDEI
                     LAGLD"
     gene            1001..2308
                     /gene="nuoF"
                     /locus_tag="PLS34_12195"
     CDS             1001..2308
                     /gene="nuoF"
                     /locus_tag="PLS34_12195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018122898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRP88259.1"
                     /translation="MARPQNQVCFAPLGCEQSWSLAVYESLGGYQAWRRILAELPSPE
                     SVVEEIKASGLRGRGGAGFPTGLKWSFMPKDPAIQKYVVCNSDESEPGTCSDRDILRF
                     NPHSVVEGMAIAGYAMRATVGYNYIRGEFLAEPVPRFEAAVAEAYAAGLLGCDIQGSA
                     VTFDLYTYVGAGAYICGEETALLESLEGKQGKPRFKPPFPANKGLFGQPTTINNTQTF
                     ASVPSIMRNGAAWFADLGVEGSGGTAIFSMSGHLEQPGNFEVGLGIPFSELLALAGGV
                     WKGRKLKAVIPGGSSSPVVPGEAMLTADMDYESIKKVGSMLGSGAVVVMDETTCMVRM
                     LERISRFYHMESCGQCTPCREGTGWMNRVLRRILAGQGRSEDLDLLDSVSRRIEGHTI
                     CAFGDAAAWPVQSFIKHFRPEFQYMIEHRGRSVVDGAGGTQAA"
     gene            2308..>2522
                     /locus_tag="PLS34_12200"
     CDS             2308..>2522
                     /locus_tag="PLS34_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRP88260.1"
                     /translation="MSDEIVNIEVDGVPVQARRGQMIIEVTDANAVYVPRFCYHPKLS
                     IAANCRMCLVEVERAPKPMPACATPVM"
BASE COUNT          425 a          812 c          880 g          405 t
ORIGIN      
        1 atcgcctggg acctgcgcaa gaagcagccc tacgaggtct acgcggagat ggacttcgac
       61 atcccggtcg gcgtgaacgg cgactgttac gaccgttacc tggtgcgggt ggaggaactg
      121 cggcagtcga accggatcat ccgccagtgc gtcaggtggc tgcgcgagaa cccgggcccg
      181 gtgtcgatcg acgaccacaa gatcgtgccg ccgcggcgcg tcgagatgaa ggacgacatg
      241 gaatcgctga tccatcactt caagctctac accgagggct attgcgtgcc cgaaggcgag
      301 gtctacgccg ccgtcgaggg gccgaagggc gaattcggct gctacctcgt ctcggacggc
      361 gccaacaagc cctaccgggt caagctgcgc gcaccgggct tcgtccacct ggcagcgctc
      421 gacgagatgg tgcgcggcca catgctggcc gacgtcgtcg ccatgatcgg caccatcgac
      481 gtggtgttcg gggaggtcga ccggtgagca cggtgaagcc cgccccgctg tcggcgcacg
      541 tccgcgagga gatcgacgcc tgggtcaagc gtttccccgc gggccggcag cgctcggccg
      601 tgctggcggc gctgcgcgcg gtccagcacg agaacgacgg ctacctgact acggagttga
      661 tggacggcgt tgccgactac cttgacctgc cgcccatcca ggtctacgag gtcgcaacct
      721 tctactcgat gttcgagacg aagccctgcg ggcggcacca cgtgtcgatc tgcaccaaca
      781 tctcgtgcat gttgcgtggt ggcgaggagc tgctgcgtca cgccgagcgg aagctcggta
      841 taagagcggg cgagagcacg cccgacgggc ggatttttct caagaacgag gaagagtgcc
      901 tcgccgcctg ttgcggcgcg ccgatgatga tggtcgatca cgtctaccac gaggatctga
      961 cggcggcgaa actcgacgag atcctggcgg ggctggactg atggcccgac cgcagaacca
     1021 ggtgtgtttc gcgccgctgg gctgcgagca gtcgtggagc ctggcggtgt acgagtcgct
     1081 cggcggctac caggcctggc ggcgaatcct tgccgagctg ccgagcccgg agtcggtggt
     1141 ggaggagatc aaggcctccg gcctgcgcgg ccgcggcggt gccggcttcc cgaccgggct
     1201 caagtggagc ttcatgccca aggacccggc catccagaag tacgtggtct gcaacagcga
     1261 cgagagcgag ccgggcacct gcagcgaccg cgatatcctg cgcttcaatc cgcactcggt
     1321 ggtggagggc atggcgatcg ccggctacgc gatgcgcgct acggtcggct acaactacat
     1381 ccgcggcgag ttcctcgccg agcccgtgcc gcgcttcgaa gcggcggtcg cggaggccta
     1441 tgcggccggc ctgctggggt gcgatatcca gggctccgca gtgaccttcg acctgtacac
     1501 ctacgtcggc gccggcgcct acatctgcgg cgaggagacc gcgctgctcg agtcgctcga
     1561 gggcaagcag ggcaagccgc gcttcaagcc gccgttcccg gccaacaagg gcctgttcgg
     1621 ccagccgacc accatcaaca acacgcagac cttcgcctcg gtgccgtcga tcatgcgcaa
     1681 cggcgcggcc tggttcgcgg acctcggcgt cgagggctcg ggcggcacgg cgatcttctc
     1741 catgtccggg cacctggagc agcccggcaa tttcgaggtc gggctgggca ttcctttcag
     1801 cgaactgctg gcgctggccg ggggcgtatg gaagggccgc aagctcaagg cggtgatccc
     1861 cggtggctct tcctccccgg tcgtgcctgg cgaggcgatg ctgaccgcgg acatggacta
     1921 cgagtcgatc aagaaggtcg gctcgatgct cggctccggc gcggtggtgg tcatggacga
     1981 gaccacctgc atggtgcgca tgctggagcg catcagccgc ttctatcaca tggagtcctg
     2041 cggccagtgc acgccctgcc gcgagggcac cggctggatg aaccgggtgc tgcgacgaat
     2101 ccttgcgggg cagggccgga gcgaggatct cgacctgctg gacagcgtat cgcgccgcat
     2161 cgagggacat accatctgcg ctttcggcga tgccgcggcg tggccggtgc agagcttcat
     2221 caagcacttc cgccccgagt tccagtacat gatcgagcac cggggccgca gcgtggtcga
     2281 tggcgccggc gggacgcagg ccgcatgatg agcgacgaaa tcgtcaacat cgaggtggac
     2341 ggcgtgccgg tgcaggcgcg gcgcggccag atgatcatcg aggtcaccga cgcgaacgcg
     2401 gtgtacgtgc ctcgcttctg ctatcacccc aagctcagca ttgcggccaa ctgccgcatg
     2461 tgcctggtgg aagtggaacg cgcgcccaag ccgatgcccg cctgcgccac gccggtcatg
     2521 ga
//
LOCUS       DAOMPG010000301         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16577_length_2520_cov_3.744016, whole genome shotgun sequence.
ACCESSION   DAOMPG010000301 DAOMPG010000000
VERSION     DAOMPG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16577_length_2520_cov_3.744016"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1078
                     /locus_tag="PLS34_12205"
     CDS             125..1078
                     /locus_tag="PLS34_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HRP88261.1"
                     /translation="MSTRPVIAVIGATGAQGGGLARAILADPARQFTLRAITRKPDSP
                     AAAALAQAGAEVVQGDLDDPATLAHAFAGAQGLFAVTNFWEHFSPERELGQAGHIAAA
                     ARAAGVGHVIWSTLEDTRREVPLDDARMPTLMGRYKVPHFDAKGEADALFDGLPTTFL
                     LTSFYWDNLIHFGMGPQRGEDGVLRFVLPMGDRKLPGIAAADIGPCALGVFRQGAAAI
                     GRRVGIAGEHLSGAEMAAELARVLGEPVQHVAMPFADYAALGFPGADDLANMFHYKHD
                     FNEAFCAARPVAQARALHPGLLSFSQWLERNVEALRAPARA"
     gene            complement(1142..1867)
                     /locus_tag="PLS34_12210"
     CDS             complement(1142..1867)
                     /locus_tag="PLS34_12210"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP88262.1"
                     /translation="MYRRHSAFNLAPGGHQRFGVRSRIVALFADMRGFSSWSETQPLD
                     RVADLVKLQFGQVMQICDDHDHPFQKFLGDGFVLLWELEGESDLARCLGHALGAAFDL
                     HRSYGALSQSLPYAAPAGYGVGISMGEAIRIQLQPHLDESSEVDFVGYPLNCAARMQT
                     LASGYGTAVCFSTAQVLLRQPDRYLQTLVPGFGRRLLEPDAEMAQRAAVTKGLKPADR
                     TGFRHLSFVDGQEWLWQTPEVLQ"
     gene            complement(2022..2282)
                     /locus_tag="PLS34_12215"
     CDS             complement(2022..2282)
                     /locus_tag="PLS34_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HRP88263.1"
                     /translation="MALFMIERDFAAELNLDSDAVKAVEEINADCGVKWLYSFLSADK
                     RKTYCLYEALNSEAVRRAARKANLPADAIIELNGEVRPEQFH"
     gene            complement(2309..>2520)
                     /locus_tag="PLS34_12220"
     CDS             complement(2309..>2520)
                     /locus_tag="PLS34_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP88264.1"
                     /translation="AAADYPDGLTQREVEVLQLLAIGRSNKDISMVLAISLSTVATHV
                     RSILGKTGCANRTEAAAYANRHQLN"
BASE COUNT          356 a          869 c          906 g          389 t
ORIGIN      
        1 ttcggctgtg gggggccggc ggcgctggcc ggctgcgggg ccggctgcgc tggccaccag
       61 ccgatctgct ataccggtac ccgtcatatc cagctgcgcc attgagccat ccagggggcc
      121 cgccatgtcg actcgacccg tcattgcagt catcggcgcc accggcgccc agggcggcgg
      181 ccttgcgcgc gccatcctcg ccgacccggc ccggcagttc acgctgcgcg ccatcacccg
      241 caagcccgac tcgccggcgg ccgccgcgct cgcgcaggcc ggcgccgagg tcgtgcaggg
      301 cgacctggac gatccggcca cgctggccca cgccttcgct ggcgcgcagg gcctgtttgc
      361 ggtgaccaac ttctgggagc acttctcgcc tgagcgggag ctcggccagg cagggcacat
      421 cgctgccgcc gcgcgcgcgg ccggcgtcgg gcacgtcatc tggtcgacgc tcgaggacac
      481 gcgccgcgag gtgccgctcg acgatgcgcg catgcccacg ctgatgggcc gctacaaggt
      541 gccgcacttc gacgccaagg gcgaggcgga cgcgctgttc gacggcctgc cgacgacgtt
      601 cctgctgacc tcgttctact gggacaacct catccacttc ggcatgggac cgcagcgcgg
      661 cgaggacggc gtgctgcgct tcgtgctgcc gatgggtgac cgcaagctgc cgggcatcgc
      721 ggcggccgac atcgggccct gtgcgctggg cgtgtttcgc cagggcgcgg ccgcaatcgg
      781 ccggcgggtg ggcatcgccg gggagcacct gagcggcgcg gagatggccg cggagctggc
      841 ccgcgtgctg ggcgagccgg tgcagcatgt cgccatgccc ttcgcggact acgcggcgct
      901 cggcttcccg ggcgcggacg acctggcgaa catgttccac tacaagcacg acttcaacga
      961 ggcgttctgc gcggcgcgcc cggtggccca ggcccgcgcg ctgcacccgg gcctgctgag
     1021 cttcagccag tggctggagc ggaacgtgga ggccctgcgg gcaccggcca gggcctgagt
     1081 cgcgcggggg atctcgctcg tccggcgctc gtccgaagcg cggccggccc ggacgctgcg
     1141 cctactgcag cacttccggc gtctgccaga gccactcctg gccgtccacg aaggacaggt
     1201 gccggaagcc ggtccggtcg gcgggcttga gccccttggt gacggcggcg cgctgcgcca
     1261 tctctgcgtc cggctcgagc agccggcggc cgaagcccgg cacgagagtc tgcaggtatc
     1321 gatccggctg gcgcagcagg acctgggcgg tgctgaagca cacagcggtg ccgtagcccg
     1381 aggcgagggt ctgcatccgc gcggcgcagt tcagcgggta gccgacgaaa tccacctcgc
     1441 tcgactcgtc caggtgcggc tggagctgga tgcggatcgc ctcgcccatc gagatgccga
     1501 cgccgtagcc ggccggcgcg gcatagggaa gcgactggct gagcgccccg tagctcctgt
     1561 gcaggtcgaa ggcggcaccg agcgcgtggc cgaggcagcg cgccaggtcg ctctcgccct
     1621 ccagttccca cagcagcacg aagccgtcgc cgagaaactt ctggaagggg tggtcgtggt
     1681 cgtcgcaaat ctgcatgacc tggccgaact gcagcttgac gaggtcggcg acgcgatcga
     1741 gcggctgcgt ctcgctccag ctggagaagc cgcgcatgtc ggcgaacagc gcgacgatgc
     1801 gcgagcgcac gccgaagcgc tggtggccgc cgggcgccag gttgaatgcc gagtgtcgtc
     1861 ggtacattga atcctgcact tgcacggcag tgacgcctgg cgggtatcaa taacactgtc
     1921 gcgcccgctc gccaagcagc acctcgcgtg ccgcgcccgc cgcggcggaa ggctgacggc
     1981 gcgcgccgga cggaacgggt ggcgtggccc gccgcccgga ctcagtggaa ctgctcgggc
     2041 ctgacctcgc cgttcagctc gatgatggcg tccgcgggca aattggcctt gcgcgccgcg
     2101 cgccgcacgg cctcggagtt gagcgcctcg tagagacaat aggtcttgcg cttgtcggcg
     2161 gacaggaaag agtagagcca cttcacgccg caatcggcgt tgatctcctc tacggccttc
     2221 acggcgtccg agtcgaggtt cagctcggcg gcgaaatcgc gctcgatcat gaaaagtgcc
     2281 atatcgtttc ggtctcccgt ggggtccgtc agttgagctg gtgccggttg gcgtaggcgg
     2341 ccgcttccgt gcggttggcg cagccggtct tgcccaggat actgcgcacg tgggtcgcaa
     2401 ccgtgctcag gctgatcgcc agcaccattg atatgtcctt gttgctgcgg ccgatggcga
     2461 gcagttgcag cacctcgacc tcgcgttgcg tcaatccgtc cggataatca gcggcggcgt
//
LOCUS       DAOMPG010000302         2485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_16858_length_2485_cov_3.698765, whole genome shotgun sequence.
ACCESSION   DAOMPG010000302 DAOMPG010000000
VERSION     DAOMPG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_2485_cov_3.698765"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLS34_12225"
     CDS             <1..475
                     /locus_tag="PLS34_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88265.1"
                     /translation="NDPDQNVRLAAFEAMVGSDDSLMRELALDAGINSTDQVLRGLAL
                     RHAVLGLSHLTITLAVDDSASKPIQESTREFLAKSGNSYVMGIDPKTKDLSTGAFNLP
                     ASSSYRGNVSGQVVTFKDAYHTGELHLQEDNTLAGTLHYQRGSTTHQFKATAPLR"
     gene            complement(498..1739)
                     /locus_tag="PLS34_12230"
     CDS             complement(498..1739)
                     /locus_tag="PLS34_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88266.1"
                     /translation="MSDRVALRITRTGCMAGAGMPRGTRAAAACWLLLLAGAPVWAQQ
                     AVVRSYDAPELEVGQAWLMHSAGRCFAVLPYHVAEETSVPSLLREGARGLRGEAVEVV
                     DLGDDAAIAALSGGISQDCGYSIGSIPRSVDRRLRDGGLGTLRSVNGDGTLGRLAVTV
                     VDDDGAGLLRVMPTMEGERIRKGHSGSLLLLGDQPVGMLLSVHARSGVGTVMRTDALL
                     AKVDGFVRGGMAAAGSDDGRGSSDVAAAGPVPAAVPSASAAAGASTPAPAALTASTSG
                     WQVSGWNVDPLGSDHIAARLTSGGAEGYWAARVPRWPAAVELGGPDAVRIVRGVEFLA
                     GEAGDSAALPARVQVMTSVNAERPAWRAVGSGALEFVDGVARLEFLPTRARMLRVEIY
                     TTQGAPAEVALGRLRVMEGER"
     gene            complement(1736..>2485)
                     /locus_tag="PLS34_12235"
     CDS             complement(1736..>2485)
                     /locus_tag="PLS34_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88267.1"
                     /translation="IEEVALRLPAMPGASPGYAVRLDVERDGSLAVQISSEDDGTAPL
                     VLLPGGEALPLAIGQAAILRFPTPRDGELPMRLLLPFVGAGSIGKDVSWREPTLLRSG
                     TVSLYTRSEEAAGGRELVTTTELLPGDRVDLGQRDGRGSATKGFVHFDLRPGPPEPAA
                     MTVVALGEADAVRIVRFGDQGYSFSPGLFAEVTRHRAVATWAVLIVSLLGFIAIVRDD
                     QGLDEGRFAAWRRKFATDWRAFRGRPGGERE"
BASE COUNT          404 a          997 c          781 g          303 t
ORIGIN      
        1 taacgacccg gaccagaacg tgcgactggc ggcgttcgag gcgatggtcg gcagcgacga
       61 ctcgctgatg cgcgaactgg cgctcgacgc cggcatcaac agcacggacc aggtgctgcg
      121 cgggctggcg ctgcgccatg ccgtgctcgg cctctcgcat ctaaccatca cgctggcggt
      181 ggacgactcc gcttcgaagc caatccagga aagcacacgt gaattcctgg cgaagagcgg
      241 caacagctac gtgatgggca tcgatccgaa gacgaaggac ctgtcgaccg gcgcattcaa
      301 cctgcccgcc agctcgagtt accgcggcaa cgtgagcggc caggtggtca ccttcaagga
      361 cgcctaccac accggcgagc tgcacctgca ggaggacaac accctggccg gcacattgca
      421 ttaccagcgg ggctccacaa ctcaccagtt caaggcaacg gccccgcttc gctgacgcat
      481 cagcccaccg gcgcgcctca acgctcgcct tccatgacgc gcagccgacc gagcgccacc
      541 tcggccggcg cgccctgcgt cgtgtagatt tccacgcgca gcatccgcgc gcgcgtcggc
      601 aggaactcca gccgcgccac gccgtcgacg aactccagcg ctcccgagcc gaccgcacgc
      661 catgccgggc gctcggcatt caccgaggtc atcacctgca cgcgtgccgg cagggccgca
      721 gagtcgcccg cctcgcccgc caagaattcc acgccacgca cgatgcgcac cgcatcgggc
      781 ccgccgagtt cgaccgccgc cggccagcgc ggcacgcgcg ccgcccaata cccctcggcg
      841 ccaccgctcg tgagccgcgc cgcgatgtga tccgacccca gcggatctac gttccagccc
      901 gagacctgcc accccgaagt cgacgccgtc aaggccgccg gggcgggcgt ggacgcaccg
      961 gccgctgcgc tcgcggacgg caccgccgcc ggcaccggtc cggccgcggc gacgtcggac
     1021 gacccgcggc cgtcatcaga gccggccgcc gccatcccgc cgcgcacgaa accgtccacc
     1081 ttggccagca gtgcatcggt gcgcatcacc gtgccgacgc cgctgcgcgc atgcaccgac
     1141 agcagcatgc cgacgggctg gtcgccgagc agcagcaggc tgccgctgtg gcccttgcga
     1201 atgcgctcgc cctccatcgt cggcatcacg cgcagcaatc ccgcgccgtc gtcgtccacc
     1261 accgtcaccg ccagccgccc gagcgtgccg tcgccgttca ccgagcgcag cgtgccgagc
     1321 ccgccgtcgc gcagccggcg gtccaccgag cgcgggatgc tgccgatgct gtagccgcaa
     1381 tcctggctga tgccgccgga gagcgccgcg atggccgcat cgtcgccgag atcgaccacc
     1441 tccacggcct cgccgcgcag gccgcgcgca ccctcgcgca gcagcgacgg cacgctcgtc
     1501 tcctccgcca cgtggtaggg cagcaccgcg aaacagcgcc cggcggaatg catcagccag
     1561 gcctggccca cttcgagttc gggcgcatca taggagcgca ccaccgcctg ttgcgcccac
     1621 accggcgcgc ccgccaacaa caggagccag catgcggccg ccgcccgcgt cccgcgcggc
     1681 attcccgccc ctgccatgca accggtcctg gtgatgcgca aggccacgcg atcgctcatt
     1741 cccgttcccc gcccggccgg ccacgaaacg cccgccagtc cgtcgcgaac ttccggcgcc
     1801 acgccgcgaa gcgtccctcg tccaggccct ggtcgtcgcg cacgatggcg atgaagccca
     1861 gcagcgagac aatcaacacc gcccacgtgg cgaccgcgcg atgccgcgtc acttctgcga
     1921 acagtcccgg cgagaagctg tagccctggt cgccgaaacg cacgattcgc accgcatccg
     1981 cctcccccag tgcaaccacc gtcatggccg ccggctcggg cgggccgggc cggaggtcaa
     2041 aatgcacgaa gcccttggtg gcgctgccgc gtccgtcgcg ttggccgaga tcgacacgat
     2101 cgccgggcag cagttcggtg gtcgtgacca gctcgcgacc gccggcggcc tcctcggaac
     2161 gcgtgtacag cgataccgtg ccgctgcgca gcagcgtcgg ctcgcgccac gacacgtcct
     2221 tgccgatcga gcccgcgccc acgaacggca gcagcaggcg catcggcagc tcgccgtcgc
     2281 gcggcgtcgg aaaacgcagg atggcggcct ggccgatcgc cagcggcagc gcttcgccgc
     2341 ccggcaacag caccagcggc gccgtgccgt cgtcctcgct gctgatctgc acggccaggc
     2401 tgccgtcgcg ctccacgtcg aggcgcacgg catagccagg gctcgcgccc ggcatggccg
     2461 gcagccgcag cgccacttcc tcgat
//
LOCUS       DAOMPG010000303         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17102_length_2454_cov_3.520217, whole genome shotgun sequence.
ACCESSION   DAOMPG010000303 DAOMPG010000000
VERSION     DAOMPG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17102_length_2454_cov_3.520217"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLS34_12240"
     CDS             complement(<1..987)
                     /locus_tag="PLS34_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88268.1"
                     /translation="MSPAQRTTPPRDAAAHDSTLSPAAFERALKASGAGIAVLSTDPA
                     FIETVWDVGHEHPSLVVADDWPGLLDAVVRGFCNIVLLDVDQVGDDLSRRLAELERCN
                     PPPVVVAAGENRRAPEMMRMHAERRIHRLLVKPATPGKLQLLLGAALTHSRRPRELEN
                     PPEPAGPNYRAPREAASGGMRRRMITLSVGMAVVFAAVVAAGLGWKSQRADVPDVAPA
                     ALRSDPPAAMESRAANVEPRAGRIERAVESAASQTMMGPPVELAGPPDREALYARVER
                     ALLEDDLPAAGAALDELRLVDPGSTRLAFLDAQLERAHMLRAAAEAPVAPVAA"
     gene            complement(1081..>1350)
                     /locus_tag="PLS34_12245"
     CDS             complement(1081..>1350)
                     /locus_tag="PLS34_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88269.1"
                     /translation="SRAVILRSLGGRLGRGGLALLCQGLPQLVRVGEDIIQLDPEAPD
                     FGDAPVLCRVKVVNETPLVPDLERLERQVAELLEGESPAGARPGN"
     assembly_gap    1351..1360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1361..1585)
                     /locus_tag="PLS34_12250"
     CDS             complement(<1361..1585)
                     /locus_tag="PLS34_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP88270.1"
                     /translation="MADEKDLLHSLLIPLVGRRLVVPRACVAEVIGLGRFKLREHEPD
                     WLLGDVAWGDRVVPLLSFEAACGDPVPEIGG"
     gene            complement(1578..>2089)
                     /locus_tag="PLS34_12255"
     CDS             complement(1578..>2089)
                     /locus_tag="PLS34_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP88271.1"
                     /translation="IAALIRARAGAPDVVPPSARAPKEDDRTFVMVVDDSITVRRVTE
                     RLLERNGMRVITAKDGVDAVSQLQEHLPDIMLLDIEMPRMDGYEVASHVRNDPRLRHI
                     PIVMITSRVGEKHRNRALEVGVDHYLGKPYQENDLLSAISSLVGERAGAGDDAPAAAP
                     GEDDGDRDG"
     assembly_gap    2090..2099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2100..>2454)
                     /locus_tag="PLS34_12260"
     CDS             complement(<2100..>2454)
                     /locus_tag="PLS34_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP88272.1"
                     /translation="ALVLRAGEEWYALPLPAVEGVVRVSAAELPRYLGPHSIPFRYGD
                     EEYLFEHIGALVDGYGSPPPDTGSVPAILVRAGERPTALLTDEMLGAREIVVKPLGPQ
                     LAGIRGIAGATVLGDG"
BASE COUNT          317 a          907 c          881 g          329 t
ORIGIN      
        1 cgccgcaacc ggcgcaaccg gtgcctctgc cgccgcgcgc agcatgtggg cgcgctcgag
       61 ctgggcgtcg aggaatgcca gccgcgtgct gcccgggtcg accagtcgca actcgtcgag
      121 cgcggcgccc gccgcgggca ggtcgtcctc gagcagggcg cgttccaccc gggcatacag
      181 cgcctcgcgg tccggcggtc ccgccagctc gaccggcggg cccatcatcg tctgcgacgc
      241 cgccgactcg acggcgcgct cgatgcgccc ggcgcgcggc tcgacgttgg cggcccggga
      301 ttccatcgcg gccggcgggt ccgaccgcaa cgccgccggc gcaacgtcgg gcacgtccgc
      361 acgctgcgac ttccagccca ggccggcggc cacgaccgcc gcgaacacca cggccatgcc
      421 gaccgacagc gtgatcatgc gccggcgcat gccgccggac gcggcttccc gcggcgcccg
      481 gtaattcggc ccggccggct ccggtggatt ctcgagctca cgtggccggc gactgtgggt
      541 gagcgcggcg ccaagcagca gctgcagctt gccgggcgtg gccggcttca ccagcaaccg
      601 gtggatgcgg cgctcggcgt gcatgcgcat catctcgggc gcgcgccggt tctcgcccgc
      661 ggcgaccacc accggcggcg ggttgcagcg ctcgagctcg gcgagccggc ggctgaggtc
      721 gtcgcccacc tggtcgacgt ccagcagcac gatgttgcag aagccgcgca ccacggcgtc
      781 gagcagcccg ggccagtcgt ccgccaccac cagcgaggga tgctcgtggc cgacgtccca
      841 gaccgtctcg atgaaagcgg ggtcggtcga cagcaccgcg atccccgcgc cgctggcctt
      901 cagcgcgcgc tcgaaggccg ccggcgacag cgtgctgtcg tgggcggcgg cgtctcgcgg
      961 cggtgtcgtt cgctgggctg ggctcattgg cgcgtccact cgaaaggccg gcgcctgggg
     1021 caccgggctg gcagtcatca agctatatga cggccgggcg cgcgaaatgt gtagcggttc
     1081 tcagtttccc ggtctggcgc cggccgggct ttcgccctcc agcagctccg ccacctggcg
     1141 ctccagccgt tccaggtcgg gcaccagcgg cgtctcgttg accaccttga cgcggcacag
     1201 caccggggcg tcgccgaaat ccggcgcctc gggatcgagc tggatgatgt cctcgccgac
     1261 ccgcaccagc tgcggcaggc cctggcagag cagcgccagc ccgccgcggc cgagccggcc
     1321 gccgaggctg cgcaggatca cggcgcgact nnnnnnnnnn cccgccgatc tcgggcaccg
     1381 gatcgccgca ggcggcctcg aaggacagca gcggcaccac gcggtcgccc cacgccacgt
     1441 cgccgagcag ccagtcgggc tcgtgctcgc gcagcttgaa gcggcccagg ccgatgacct
     1501 cggcgacgca ggcgcgcggc accaccaggc gacggccgac cagcgggatg agcaggctgt
     1561 gcagcaggtc cttctcgtca gccatcgcgg tctccgtcgt cctcgccggg cgcggctgcc
     1621 ggggcgtcat cgcccgcgcc ggcccgctcg ccgaccaggc tgctgatggc gctcagcagg
     1681 tcgttctcct ggtagggctt gccgaggtaa tggtccacgc cgacttccag cgcgcggttg
     1741 cgatgcttct cgccgacgcg cgaggtgatc atgacgatcg ggatgtgccg caggcgcgga
     1801 tcgttgcgca cgtggctggc gacttcgtag ccgtccatgc gcggcatctc gatgtcgagc
     1861 agcatgatgt cgggcaggtg ctcctgcagc tgcgagaccg cgtcgacgcc gtccttcgcg
     1921 gtgatgacgc gcatgccgtt gcgctccagc agccgttcgg tcacgcggcg cacggtgatc
     1981 gagtcgtcca ccaccatgac gaaggtgcgg tcgtcctcct tgggggcgcg cgcgctcggc
     2041 ggcaccacgt cgggcgctcc ggcgcgggcg cggatcagcg cggcgatgtn nnnnnnnnnc
     2101 cgtcgcccag cacggtggcg ccggcaatgc cgcggatgcc ggcaagctgc gggccgagcg
     2161 gcttcaccac gatctcgcgc gcgcctagca tctcgtcggt gagcagcgcg gtcgggcgct
     2221 cgccggcgcg caccaggatg gcgggcaccg agccggtgtc gggcggcggc gagccgtagc
     2281 cgtccaccag cgcgccgatg tgctcgaaca ggtattcctc gtcgccgtag cggaacggga
     2341 tcgaatgcgg gcccaggtag cgcggcagct cggcggcgct gacgcgcacc acgccctcca
     2401 ccgccggcag cggcaaggcg taccactctt cgccggcgcg cagcaccagc gcct
//
LOCUS       DAOMPG010000304         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17143_length_2450_cov_5.247182, whole genome shotgun sequence.
ACCESSION   DAOMPG010000304 DAOMPG010000000
VERSION     DAOMPG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17143_length_2450_cov_5.247182"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLS34_12265"
     CDS             <1..726
                     /locus_tag="PLS34_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932933.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRP88273.1"
                     /translation="GLNLMFETREGILKHCSARNARALGELGRRFLEGLQPGLEAQLA
                     NLADEIAYNNHDVDDGLRAGLLNTEELREAALFREQHDAVRARWPKLAGRRLVHEIVR
                     RMIGRVVEDLVAASQLELQAAAPDSIEAVRRAGRPLIRFSDEVGAAHLELKRFLRDRL
                     YRHNRVIRMTTKAQRTVRELFEAFMQEPRLMPDDHALEAARKEAAQGAAGRARAVADY
                     IAGMTDRFAILEHGRLFDPRART"
     gene            830..1543
                     /gene="pyrF"
                     /locus_tag="PLS34_12270"
     CDS             830..1543
                     /gene="pyrF"
                     /locus_tag="PLS34_12270"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRP88274.1"
                     /translation="MTPKDIPVDERLIFALDVPDAWQARELVQELDDSVRFYKLGLEL
                     FMAGGYFELLDWLAGRGKRIFVDLKFFDVPATVARAVERLNGRGVTFCTVHGNQGIME
                     AAVAAREDVKVLAVTALTSLDRGDLEDLGFQCDVPELVLSRARRALEAGCDGVVSSGL
                     EAARLREHLDARLLVVTPGIRPVENRETDDQKRVVTVDQAFALGADYIVVGRPIRDAD
                     DPRAAAEAIQASIAKATGA"
     gene            1540..>2450
                     /gene="hemE"
                     /locus_tag="PLS34_12275"
     CDS             1540..>2450
                     /gene="hemE"
                     /locus_tag="PLS34_12275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRP88275.1"
                     /translation="MTVELKNDRFLRALARQPVDRTPVWMMRQAGRYLPEYRATRARA
                     GSFMQLCKSPELACEVTLQPLERYPLDAAILFSDILTVPDAMGLGLYFEGGEGPRFER
                     TIERDADVDALATPDPENELRYVMDAVRTIRRELGGRVPLIGFAGSPWTVATYMVEGG
                     TSKNFSRIKGMLYDKPALLHRLLERVTRTTLDYLNAQVAAGAQAVMVFDTWGSILTPK
                     TYRGFSLDYMQRIVDGVTREAGGRKVPVVLFTKGGGPWLEAMADTGCDALGVDWTTDL
                     AEARARVGDRVALQGNLDPAVLYASPEV"
BASE COUNT          373 a          835 c          884 g          358 t
ORIGIN      
        1 ggcctgaacc tgatgttcga aacccgcgag ggcatcctca agcattgctc ggcgcgcaac
       61 gcgcgcgcgc tgggcgaact gggccgccgc tttctcgagg gcctgcagcc tggcctggag
      121 gcgcagctgg ccaacctggc ggacgagatt gcatacaaca accacgacgt cgacgacggc
      181 ctgcgggccg gcctgctgaa caccgaggag ctgcgcgagg ccgcgctgtt tcgcgaacag
      241 cacgacgcgg tgcgggcgcg ctggccaaag ctcgccggcc gccggctggt gcacgagatc
      301 gtgcggcgca tgatcggccg cgtggtcgag gacctggtgg ccgcgtcgca gctcgaactg
      361 caggcggccg cgcccgactc gatcgaggcg gtgcgccggg ccgggcggcc gttgatccgt
      421 ttcagcgacg aggtgggcgc cgcgcacctc gagctgaagc gcttcctgcg cgaccgcttg
      481 tatcgccaca accgcgtgat ccgcatgacg accaaggccc agcgcacggt gcgggagctg
      541 ttcgaggctt tcatgcagga gccccggctg atgcccgacg accatgcact cgaggcggcc
      601 cgcaaggagg ccgcgcaggg ggctgccggc cgcgcgcggg cggtcgccga ctacatcgcc
      661 ggcatgaccg accgcttcgc gatcctcgag cacggccggc tgttcgaccc gcgcgcccgt
      721 acttgaggcc ggcgccagcc ggcaaggccg gcattgcggc cttccccgac gctgccccct
      781 ccccgccgga tgctagaatc gcggcctcat tcaaggccgg accgcattca tgaccccgaa
      841 ggacatcccc gtcgacgagc ggctgatatt tgcgctcgac gtgcccgacg cgtggcaggc
      901 gcgcgagctg gtgcaggagc tcgacgattc cgtgcgcttt tacaagctgg gactcgagct
      961 gttcatggcg ggcggctatt tcgagctgct cgactggctg gcgggacgcg gcaagaggat
     1021 cttcgtggac ctgaagttct tcgatgtccc ggcgaccgtg gcgcgcgcag tggagcgcct
     1081 caacgggcgc ggcgtgacct tctgcaccgt gcacggcaac cagggcatca tggaagccgc
     1141 ggtggcggcc cgcgaagacg tcaaggtgct ggccgtgacc gcgctgacca gcctcgatcg
     1201 cggcgatctc gaggacctcg gcttccagtg cgacgtgccc gaactcgtgc tgtcgcgcgc
     1261 ccgccgggca ctggaggccg gctgcgacgg cgtggtgtcc tccgggctgg aggcggccag
     1321 gctgcgcgag cacctggacg cccgcctgct ggtggtgacg ccgggcatca ggccggtgga
     1381 gaaccgggaa acggacgacc agaagcgggt cgtcacggtc gaccaggcgt tcgcgctggg
     1441 cgcggactac atcgtggtgg ggcggccgat ccgggatgcc gacgatcccc gggccgccgc
     1501 ggaagccatc caggcaagta ttgcaaaggc gacaggcgca tgaccgtgga gttgaagaac
     1561 gaccgtttcc tgcgggccct ggcccgccag ccggtggatc gcacgccggt gtggatgatg
     1621 cgccaggccg ggcgctacct gcccgagtac cgcgccaccc gcgcccgcgc cggcagcttc
     1681 atgcagctct gcaagtcgcc cgagctggcc tgcgaggtga ccctgcagcc gctggagcgc
     1741 tacccgctgg acgcggcgat cctgttctcc gacatcctga cggtgccgga tgccatgggc
     1801 ctgggcctgt atttcgaggg cggcgagggg ccgcgcttcg agcgcaccat cgagcgcgat
     1861 gcggacgtcg atgcgctggc gacgccggat ccggagaacg agctcaggta cgtcatggat
     1921 gcggtcagga ccattcgccg cgagctcggc ggccgcgtgc cgctgatcgg cttcgccggc
     1981 agcccctgga ccgtcgcgac ctacatggtg gaaggcggca ccagcaagaa cttcagtcgc
     2041 atcaagggca tgttgtacga caagccggcc ctgctgcacc ggctgctgga gcgcgtgacg
     2101 cgcaccacgc tggattacct caacgcccag gtagccgccg gcgcccaggc cgtgatggtg
     2161 ttcgacacct ggggcagcat tctcacgccg aagacctatc gcggcttttc cctcgattac
     2221 atgcagcgca tcgtcgacgg ggtgacccgc gaggccggcg gccgcaaggt accggtggtg
     2281 ctgttcacca agggcggcgg cccgtggctg gaagcgatgg ccgacaccgg ctgcgacgcg
     2341 ctgggcgtgg actggaccac cgaccttgcc gaggcgcgcg cccgggtggg cgaccgcgtg
     2401 gccctgcagg gcaatctcga ccccgcggtg ctgtacgcgt caccggaggt
//
LOCUS       DAOMPG010000305         2417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17420_length_2417_cov_2.442422, whole genome shotgun sequence.
ACCESSION   DAOMPG010000305 DAOMPG010000000
VERSION     DAOMPG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17420_length_2417_cov_2.442422"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>408)
                     /locus_tag="PLS34_12280"
     CDS             complement(<1..>408)
                     /locus_tag="PLS34_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRP88276.1"
                     /translation="RAALPPVPEEGAPYATLDDLRECDALALGSPTRFGNMAAPLKHF
                     LDGTGETWLDGTLAGKPAGVFTSTSTMHGGQESTLLSMMLPLLHHGALLVGLPYTLQA
                     LTDTRDGGSPYGPSHVATPAAGTTLSEHEKTLA"
     assembly_gap    409..450
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<451..583)
                     /locus_tag="PLS34_12285"
     CDS             complement(<451..583)
                     /locus_tag="PLS34_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRP88277.1"
                     /translation="MIRVLVVYYSRQGSTAALARQVARGVATVPGAEAVLRTVPPVAP
                     "
     gene            complement(580..930)
                     /gene="arsC"
                     /locus_tag="PLS34_12290"
     CDS             complement(580..930)
                     /gene="arsC"
                     /locus_tag="PLS34_12290"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185914.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRP88278.1"
                     /translation="MAVTIYHNPRCSKSRQTLALLESRGVKPRVVEYLKTPPDRATIE
                     ALLRKLGIAASQLVRRKEPEFAALGLDAAGVSEERLIDAMASHPKLIERPIVVVDRRA
                     RIGRPPEAVLEILP"
     gene            1039..1347
                     /locus_tag="PLS34_12295"
     CDS             1039..1347
                     /locus_tag="PLS34_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HRP88279.1"
                     /translation="MTEFRARSRLATRCAAVAGLLLLAACASAPVQEMSDARQAIHAA
                     EEAGAAAYAPEALQDAKRLLTSAERKLQREAFQSARIDAREARRRAAEALSAVPAGDD
                     "
     gene            complement(1356..1925)
                     /locus_tag="PLS34_12300"
     CDS             complement(1356..1925)
                     /locus_tag="PLS34_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRP88280.1"
                     /translation="MSRSVLALLLLALAPVVPAQQSPEDVDPELRRVLREAVESADSF
                     TDRFDAEVWLTDMSARLARQVPDKTERMLILRLVHQYAAQFDLPPELVLAVIDVESNF
                     DRYAISHAGALGLMQVMPFWRNEIGRPDDNLHDIQTNLLYGCRILAYYLEMERGDRRK
                     ALARYNGSRGQRWYPDRVFSRLSAKWFQY"
     gene            complement(1922..>2417)
                     /locus_tag="PLS34_12305"
     CDS             complement(1922..>2417)
                     /locus_tag="PLS34_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRP88281.1"
                     /translation="QLGRKYSEAMGAVVLDADGREQPMVMGCYGIGVTRLVAAAIEQN
                     FDQRGIIWPEPIAPFQVALVPMNLQKSEAVRAAVEPLYAALLAAGVEVLLDDRDVRPG
                     VKFADIELLGIPHRVVVGERGLAEGQLEYRARRATDNESLPLEGAAQALLARVRAGMA
                     RHSP"
BASE COUNT          345 a          842 c          841 g          347 t
ORIGIN      
        1 gcgccagggt cttttcgtgc tcgctgagcg tcgtgccggc cgcgggcgtg gcgacgtgcg
       61 aggggccgta gggcgagccg ccgtcacgcg tgtcggtgag cgcctgcagg gtgtagggca
      121 ggcctaccag cagggcgccg tgatggagca gcggcagcat catgctcagc agcgtgcttt
      181 cctggccgcc gtgcattgtc gaggtcgacg tgaacacccc ggccggcttg ccggccagcg
      241 tgccgtccag ccaggtctcg ccggtgccgt ccaggaaatg cttcagcggc gcggccatgt
      301 tgccgaagcg cgtgggactg ccgagcgcca gcgcatcgca ttcgcgcagg tcgtcaagtg
      361 tggcataggg cgcgccttcc tccggcactg gcggcagcgc cgcgcgggnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcggcgccac cggcggcacc gtgcgcagca
      481 cggcctcggc gcccggcacc gtggcgaccc cccgggccac ctggcgcgcc agcgcggcgg
      541 tgctgccctg gcgggaatag tagacgacca gcacccggat caaggcagga tctcgagcac
      601 cgcctccggc gggcggccga tgcgggcccg ccggtcgacc accacgatgg ggcgctcgat
      661 gagcttggga tgcgaggcca tcgcgtcgat cagccgttcc tcggagacgc cggccgcatc
      721 gagcccgagc gcggcgaact cgggctcctt gcgccgcacc agctgacttg cggcgatgcc
      781 cagcttgcgc aacaaggcct cgatggtggc ccgatccggc ggcgtcttca ggtactccac
      841 cacgcgcggc ttgacgccgc gggactcgag cagcgcgagg gtttgccgcg acttcgagca
      901 gcgggggttg tgataaatcg tcactgccat gatttgactc cgtttttccc ggttggccgc
      961 ggccggcgcg cgcttgacgg ccggaagcgg cggctgctag tttcctcgcc ggaggatggt
     1021 cgcaagagtg ggccccgaat gaccgagttc cgtgccaggt cccgcctggc gacgcgctgc
     1081 gctgcggtgg ccggcttgct gctgctcgcc gcttgtgcca gcgcgcccgt gcaggagatg
     1141 agcgacgcgc gccaggccat ccatgcggcc gaggaagccg gcgcggcggc ctatgccccg
     1201 gaggcgctgc aggacgccaa gcgcctgctc accagcgccg agcgcaagct ccagcgggag
     1261 gccttccaga gcgcgcggat cgacgcccgc gaggcgcgcc gccgggcggc cgaggccctc
     1321 agcgcggtgc ccgccggaga cgactgaagc cgccatcagt actggaacca cttcgcgctc
     1381 aggcggctga aaacgcggtc cgggtaccag cgctggccgc ggctgccgtt gtagcgcgcc
     1441 agcgccttgc ggcggtcgcc gcgctccatt tcgaggtaat aggcgagtat ccggcagccg
     1501 tacagcaggt tggtctggat gtcgtgcaga ttgtcgtccg gccggccgat ttcgttgcgc
     1561 cagaacggca tgacctgcat cagcccgagc gcgccggcat gcgaaattgc gtagcggtcg
     1621 aaattgctct cgacgtcgat gacggcgagc accagctcgg gcggcaggtc gaactgcgcg
     1681 gcatactggt gcacgaggcg caggatcagc atccgctcgg tcttgtccgg cacctggcga
     1741 gcgagccgcg cggacatgtc cgtcagccac acttcggcgt cgaagcgatc ggtgaagctg
     1801 tcggcgctct ccacggcctc gcgcagcacg cggcgcagct ccggatcgac gtcctcgggc
     1861 gactgctgcg ccgggacgac cggggccagc gcgagcaaca gcaacgccag gaccgagcgg
     1921 ctcatggcga atggcgggcc atcccggcac gcacgcgcgc cagcagcgcc tgtgcggcgc
     1981 cctcgagcgg cagcgattcg ttgtcggtcg cgcgccgcgc ccggtattcc agctggcctt
     2041 ccgcgaggcc gcgctcgccg acgaccacgc gatgcggaat gccgagcaac tcgatgtcgg
     2101 cgaatttcac gccgggccgc acgtcccgat catcgagcag cacctcgacc ccagccgcca
     2161 gcagcgctgc gtagagcggc tcgaccgccg cgcgcaccgc ctcgcttttc tgcaggttca
     2221 tcggcaccag cgcgacctga aagggcgcga tcggctccgg ccagatgatg ccgcgctggt
     2281 cgaaattctg ctcgatcgca gcggccacca gccgcgttac gcctatgccg taacagccca
     2341 tcaccatcgg ctgttcgcgg ccgtccgcgt ccagcaccac ggcgcccatc gcctcgctgt
     2401 acttgcgccc cagctgg
//
LOCUS       DAOMPG010000306         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17492_length_2407_cov_5.197704, whole genome shotgun sequence.
ACCESSION   DAOMPG010000306 DAOMPG010000000
VERSION     DAOMPG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17492_length_2407_cov_5.197704"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /gene="bioD"
                     /locus_tag="PLS34_12310"
     CDS             <1..496
                     /gene="bioD"
                     /locus_tag="PLS34_12310"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRP88282.1"
                     /translation="ALQPAIAPHIAAAEADVRLDAAKLAAACRRAAEGAEYLVVEGAG
                     GWRVPLGPDSTLAAVAVALGLPVVLVVGLRLGCLNHALLSAAAIRADGLALAGWVANP
                     LDPDMPRREENIAALVEHLGTPPLARLPACDGRGETDRARQAAAALPADIAARLGAGG
                     ARTA"
     gene            648..1184
                     /locus_tag="PLS34_12315"
     CDS             648..1184
                     /locus_tag="PLS34_12315"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRP88283.1"
                     /translation="MAAQREVGDVSDISDRLLGQILVSANSSLTRQGAGLFLGVTAAA
                     SLVMAGALTVRGFWPVLPFAGLGLFALGVALGLNMRRGRYRECVSVYGDRIVVEKGVG
                     TVDECCQLPRQWTRVELVRARWRGHPSRLLLRWHGKSVEVGAVLTEAERTSLGLRLAE
                     LVAGSDTAQAGAAGDEEF"
     gene            1220..2047
                     /gene="coxB"
                     /locus_tag="PLS34_12320"
     CDS             1220..2047
                     /gene="coxB"
                     /locus_tag="PLS34_12320"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRP88284.1"
                     /translation="MSRTTTRWMGRVAAGAGLGLAVQASAWADWGVNMPRGVTEISNE
                     VYDLHMLIFGVCVAIAVVVFGAMIWSIVHHRKSRGIEPAKFSHSTKVEIVWTVIPIAI
                     LVAMAVPAADTLVRMEDTRNSDLTVKITGYQWMWEYEYLEEDVRFFSRLSEESNRARQ
                     LRSGIDVRQVENYLLDVDRPLVVPVGAKVRLLLTANDVIHSWWVPDFGGKKDAIPGFI
                     NEFWFQAEEVGTYRGQCAELCGRDHGFMPVVVEVVSADEYSAWLARQSPDAEVAAAR"
     gene            2071..>2407
                     /locus_tag="PLS34_12325"
     CDS             2071..>2407
                     /locus_tag="PLS34_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP88285.1"
                     /translation="MSTATHAEHGDHGHHPTGLLRWLTTTNHKDIGTMYLVFSLVMFF
                     VGGAMALVIRAELFQPGLQFVDPAFFNQMTTMHALIMIFGAVMPAFVGLANWMVPLQI
                     GAPDMALPRM"
BASE COUNT          352 a          778 c          882 g          395 t
ORIGIN      
        1 ggcgctgcag ccggccatcg cgccgcatat cgccgccgcc gaggccgacg tccggctgga
       61 cgccgccaag ctggccgccg cctgccgccg tgcggcggag ggcgcggaat acctggtggt
      121 ggaaggcgcg ggcggctggc gcgtgcccct cgggccggac agcacgctcg ccgcggtggc
      181 cgttgcgctc ggcctgccgg tggtgctggt cgtcggcctg cggctgggat gcctgaacca
      241 tgccctgctg agcgcggcgg cgatccgcgc cgacgggctg gcgctggccg gctgggtggc
      301 caacccgctg gaccccgata tgccgcgccg ggaggagaac attgcggccc tcgtcgagca
      361 tctcggcacg ccgcccctag cgcgcctgcc ggcctgcgac ggccgcggcg aaaccgaccg
      421 ggcacgccag gcggccgcgg cgctgcccgc tgatatcgcg gcgcggctgg gcgcgggcgg
      481 cgcccggacg gcctgacccg ccgggcgcgg cgcgccccga atggcggccg tttgcggggt
      541 tttttgctaa cagcctgtct gtgaaagaaa actttcccga cttggaatga gtcgatgttg
      601 cgcgccattg cggcattcac ttacgattag cccgcttttg cggcgccgtg gctgcacagc
      661 gagaggttgg cgacgtctcc gacatcagtg acaggctcct cgggcagatc ctggtcagcg
      721 cgaactcgtc gctgacccgc cagggagcgg ggctgtttct gggcgtgacc gcggccgctt
      781 ccctggtgat ggcaggcgcg ctcacggtac ggggtttctg gccggtgctg ccgttcgcgg
      841 gactcgggct gtttgcactg ggcgtggcgc tcggcctgaa catgcggcgc ggccggtatc
      901 gtgagtgcgt cagcgtgtat ggcgaccgga tcgtcgtcga gaagggcgtc ggcacggtgg
      961 atgaatgttg ccagctgccg cggcagtgga cgcgggtgga actggtgcgc gcgcggtggc
     1021 gcggccatcc cagcaggctg ctgctgcgct ggcacggcaa gagcgtggag gtcggcgcgg
     1081 tgctgaccga ggcggaacgc acaagcctgg ggctgcgcct cgcggagctg gtcgccggca
     1141 gcgacacggc gcaggcgggt gcagccggcg acgaagaatt ttgagtgaga gtgtcctgat
     1201 tcagggggag cccggactga tgtccagaac cacgacaagg tggatgggcc gcgttgcggc
     1261 aggagcgggg ctgggcctcg ccgtacaggc gtcggcatgg gccgactggg gcgtgaacat
     1321 gccgcgcggc gtgaccgaga tcagcaacga ggtctacgac ctccacatgc tgattttcgg
     1381 cgtctgcgtc gcgatcgcgg tagtggtgtt cggcgcgatg atctggtcca tcgtccatca
     1441 ccgcaaatcg cgcggcatcg agccggcgaa gttctcacac agcaccaagg tcgagatagt
     1501 ctggaccgtg attcccatcg ccatcctggt ggcgatggcg gtgccggcgg ccgacacgct
     1561 ggtccggatg gaggacaccc gcaacagcga cctcaccgtc aagatcaccg gctaccagtg
     1621 gatgtgggag tacgagtatc tcgaggagga tgtgcgcttc ttcagccggc tgtcggagga
     1681 gtccaaccgg gcccgccagc tgcgctccgg gatcgacgtg cgccaggtcg agaactacct
     1741 gctggacgtg gaccgcccgc tggtcgtgcc ggtcggcgcc aaggtccgcc tgctgttgac
     1801 cgcgaacgac gtcatccact cgtggtgggt gccggacttc ggcggcaaaa aggacgccat
     1861 tccgggcttc atcaacgagt tctggttcca ggccgaggag gtcgggacct accgcggcca
     1921 gtgtgccgag ctgtgcggac gcgaccacgg cttcatgccg gtggtcgtcg aggtggtgtc
     1981 ggccgacgag tacagcgcct ggctggctcg ccagtcgcca gacgccgaag tcgccgccgc
     2041 ccgctgagcc gatccaagga gagtcacacg atgtccactg cgacccacgc cgagcacggc
     2101 gaccacggcc accatcccac cggcctcctg cgctggctga cgaccacgaa tcacaaggac
     2161 atcggcacga tgtacctggt gttcagcctg gtgatgttct tcgtcggcgg cgcgatggcg
     2221 ctggtgatcc gcgccgagct gttccagccg ggcctccagt tcgtggatcc cgccttcttc
     2281 aaccagatga ccaccatgca cgcgctgatc atgatcttcg gcgcggtcat gccggctttc
     2341 gtgggcctgg cgaactggat ggtgccgctg cagatcggcg cgccggacat ggcgctgccg
     2401 cgcatga
//
LOCUS       DAOMPG010000307         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17632_length_2391_cov_2.750428, whole genome shotgun sequence.
ACCESSION   DAOMPG010000307 DAOMPG010000000
VERSION     DAOMPG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17632_length_2391_cov_2.750428"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLS34_12330"
     CDS             complement(<1..1157)
                     /locus_tag="PLS34_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281000.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRP88286.1"
                     /translation="MSADSLEQHTPMMRQFLRIKAEHPDILLFYRMGDFYELFYDDAR
                     RAAGLIDITLTTRGQSAGQPIPMAGVPVHSVEGYLAKLVRKGESVAICEQIGDPATSK
                     GPVERQVVRVVTPGTVTDEGLLEARREVLLGCVHAGAGGIGVAWLDLAGGRFRVARLE
                     DVEALAAELERLQLAELLVDEESRLPGFVRALPGLKTRPPWHFDLDAATRLLCRQFGV
                     ADLDGFGCAELPEAVQAAGCLLQYVLDTQRSALPHLRGLATETRDEALVMDAATRRNL
                     EIDRSLSGRHEHTLLGVLDETATPMGGRLLRRWLNRPLRDRGVLAARHEAIEKLLHDG
                     DPGALREVLRGVGDIERMLARVALRTARPRDLAGLREALARLPALRSAVVDV"
     gene            1259..1756
                     /locus_tag="PLS34_12335"
     CDS             1259..1756
                     /locus_tag="PLS34_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRP88287.1"
                     /translation="MIPDDDRLLKLAREVGVWLVARGEKLATAESCTAGWIAKAITDV
                     AGSSEWFTAGIASYADEAKTALLGVPEGLLAAEGAVSKAVVEAMAQGALERSGADRAV
                     AVSGIAGPGGGTEEKPVGTVWFAWARRDGDEVVFKTRLEHFQGDRDGIRRQAVGRALS
                     GVMMS"
     gene            1753..2304
                     /gene="thpR"
                     /locus_tag="PLS34_12340"
     CDS             1753..2304
                     /gene="thpR"
                     /locus_tag="PLS34_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRP88288.1"
                     /translation="MKLRLFFALWPAEPVRDALAAAAAPLLQACRGRRVTKRNYHLTL
                     AFLGGVPAGRLEAVRAAAATVQTEGFALSIDCHGHWPGPRVAWLGCRQLPAAADRLAR
                     ALWAALEPLGFRPDPRPFRPHLTVLRGCRACDWAGPVAPVAWPVDDFVLVNSGSDTSF
                     PGPHYEVLDRWPLKKGTFPNSAQ"
BASE COUNT          316 a          854 c          873 g          348 t
ORIGIN      
        1 acgtcgacga ccgccgagcg caacgccggc agccgcgcca gcgcctcgcg caggccggcg
       61 aggtcgcgcg gccgcgcagt gcgcaatgcg acgcgcgcca gcatgcgctc gatgtcgccg
      121 acgccccgca gcacctcgcg cagcgccccg ggatcgccat cgtgcagcag cttctcgatg
      181 gcctcgtggc gcgccgccag cacgccgcgg tcacgcagcg gccggttgag ccagcggcgc
      241 agcagccgcc cgcccatcgg cgtggcggtc tcgtccagca cgccgagcag ggtgtgctcg
      301 tgccggcccg acaggctgcg gtcgatctcg aggttgcggc gcgtggcggc gtccattacc
      361 agcgcctcgt cgcgcgtctc ggtggccagc ccgcgcaggt ggggcagggc gctgcgctgc
      421 gtgtcgagca cgtactgcag caggcagccg gcggcctgca cggcctccgg cagttccgca
      481 cagccaaaac cgtcgagatc ggccacgccg aactgcctgc acagcagccg tgttgcggcg
      541 tcgaggtcga aatgccaggg cgggcgggtc ttcagtccgg gcagcgcgcg cacgaagccc
      601 ggcaggcggc tttcttcgtc gaccagcagc tcggcgagct gcagccgctc cagctcggcg
      661 gccagcgcct cgacgtcctc gagtcgcgcg acgcggaagc gcccgcccgc caggtcgagc
      721 cacgccactc cgatgccgcc ggcgcccgcg tgcacgcagc cgagcagcac ctcgcggcgc
      781 gcctcgagca ggccctcgtc ggtcaccgtg ccgggcgtga caacccgcac cacctgccgc
      841 tccaccgggc ctttggaggt cgccgggtcg ccgatctgct cgcagatcgc caccgactcg
      901 cccttgcgca ccagcttggc gagataacct tcgacgctgt gcaccggcac gccggccatc
      961 gggatgggct ggccggcgct ctggccgcgc gtcgtcaggg tgatgtcgat cagcccggcg
     1021 gcgcggcgcg cgtcgtcgta gaacagctcg tagaagtccc ccatccggta gaacaggagg
     1081 atgtcggggt gctcggcctt gatgcgcagg aactggcgca tcatcggtgt gtgctgttcg
     1141 aggctgtcgg cgctcatggg gtttccgtcg cgagaccgag catgctagca ggtcgggtgc
     1201 ctctttttct gccgcgcgga tggtggcagg ctacgcggca gcggagggga ggtcccggat
     1261 gattcctgac gacgatcgcc tgttgaaact cgcccgcgag gtcggcgtct ggctggtggc
     1321 gcgcggcgag aagctggcca ccgcagaatc ctgcaccgcc ggctggatcg ccaaggcgat
     1381 caccgacgtg gccggcagct cggaatggtt caccgccggg atcgcgagct acgccgatga
     1441 ggccaagacg gcgctgctcg gcgtgccgga gggtctgctg gcggccgagg gcgcggtcag
     1501 caaggcggtc gtcgaggcca tggcgcaggg cgcgctggag cgcagcggtg cggatcgcgc
     1561 cgtggcggtc agcggcatcg ccggccccgg cggcggcacc gaggagaagc ccgtcggcac
     1621 ggtgtggttc gcctgggcgc gccgcgacgg cgacgaagtg gtgttcaaga cccggctgga
     1681 gcatttccag ggcgaccgcg acggcatccg gcgccaggcc gtgggccgcg ccctgtccgg
     1741 ggtcatgatg agttgaagct gcggctgttc ttcgcgctgt ggcccgccga gccggtccgc
     1801 gacgcgctgg ccgccgccgc cgcgcccctc ctccaggcct gccgtggccg gcgtgtgacc
     1861 aaacgaaact accacctgac gctggccttt ctcggcggcg tgcccgccgg ccggctggag
     1921 gccgtgcgcg cggccgcggc gacggtgcag accgagggat tcgctctctc gatcgattgc
     1981 cacggacact ggccggggcc gcgggtggcc tggctgggct gccggcagct gccggcagcc
     2041 gccgatcgcc tggcccgggc gctgtgggcg gcgctcgagc cgctgggctt taggcccgac
     2101 ccacggccgt tccggccgca tctcacggtg ctgcgcggtt gccgtgcctg cgactgggcc
     2161 ggcccggtgg ccccggtcgc atggccggtc gacgacttcg tgctcgtgaa ttcggggtca
     2221 gacaccagtt tcccgggccc gcactacgag gtcctcgacc gctggccgct gaaaaagggg
     2281 acattcccca attccgcaca gtgaaatggg gaatgtcccc tttttcggtc gatatgcccg
     2341 ctgccccgcg gggccggatg tgaaataatc cacctcttcc cgaactcacc g
//
LOCUS       DAOMPG010000308         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17641_length_2389_cov_4.543273, whole genome shotgun sequence.
ACCESSION   DAOMPG010000308 DAOMPG010000000
VERSION     DAOMPG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17641_length_2389_cov_4.543273"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PLS34_12345"
     CDS             complement(<1..1417)
                     /locus_tag="PLS34_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRP88289.1"
                     /translation="MKISWSEYDPGKGWDELLKGPGKPRAHARQLTKYLSSLSPSQLS
                     RRQEAAERAIIEMGITFTVYSEGQNIDRAWPFDIIPRVMPAEEWRRIEAGLKQRLAAL
                     NMFINDIYNEQRILDDGRFPRELIETSGNFLRECMGVKPAHGVWAHICGSDLVRDADG
                     TVYVLEDNLRVPSGVSYMLENRIVTKQVFPELFAHSRILPVDDYPSLLFDMLASLSPR
                     PLDYPEVVVLTPGIYNSAYFEHAYLAQAMGAELVEGGDLVVADDDCVYMRTIAGLERV
                     DVIYRRVNDTFMDPEVFRADSMLGVPGLMRAWRAGNVAIANAPGSGVADDKLVYAFVP
                     DIIRYYLDQDPILPNVPTYACVNKDDQRFVLDNLAELVVKPVNESGGYGMLIGPAATR
                     EDIDKFARLVKRQPRNYVAQPVLNLSTAPTLVRGRLEPRHLDLRPFVLQGDRSAVTAG
                     GLTRVALVKGSLVVNSSQGGGS"
     gene            complement(1487..>2389)
                     /locus_tag="PLS34_12350"
     CDS             complement(1487..>2389)
                     /locus_tag="PLS34_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88290.1"
                     /translation="SGEVHWMRHMIDAHEARAMASGARLVHACGFDSIPSDLGCLFLQ
                     QRAIERYGRPCSSVKLFVRRMQGTFSGGTVASMLNALEAARRSPEDRRIMGNPYALNP
                     AGERSGPDGSDQARPLRDPDADGWTAPFVMAAVNTRIVRRSHALLGWPWGRRFRYAES
                     VITGDGVAGLARATALTAGLAGFMAAASVGPARAVMNRLFLPQPGQGPDEAAREAGGF
                     EMLLVGRHPAAPRRHIQARVTGRRDPGYGATARMLGEAAMCLALDGDRLPVAGGFWTP
                     ATAFGARLIGRLAQHADIRFTIDV"
BASE COUNT          371 a          814 c          835 g          369 t
ORIGIN      
        1 tgctgccgcc gccctggctg gagttgacca ccagcgagcc cttcaccagc gcgacgcgcg
       61 tgaggccgcc ggcggtcacg gcactgcggt cgccctgcag gacgaagggg cgcaggtcga
      121 ggtgccgcgg ctcgagccgg ccgcgcacca gggtcggcgc cgtcgacagg ttgagcaccg
      181 gctgcgccac gtagttgcgt ggttgacgct tgaccaggcg cgcgaacttg tcgatgtctt
      241 cacgcgtcgc ggccggtccg atgagcattc cgtaacctcc cgactcgttc accggcttga
      301 cgaccagttc ggccaggttg tccagcacga agcgctgatc gtccttgttc acacaagcgt
      361 aggtcggcac gttcggcagg atcgggtcct ggtcgaggta gtaacggatg atgtccggca
      421 cgaaggcgta caccagcttg tcgtcggcca cgcccgagcc gggggcgttg gcgatcgcca
      481 cgttgccggc gcgccaggcg cgcatcagcc cgggcacgcc gagcatggag tcggcgcgaa
      541 atacctctgg atccatgaag gtgtcgttga cgcgccggta gatgacgtcg acgcgctcca
      601 gaccggcgat ggtgcgcatg tagacgcaat cgtcgtcggc caccaccagg tcgccgccct
      661 cgaccagctc ggcacccatg gcctgcgcca ggtaggcgtg ctcgaaatac gccgagttgt
      721 agatacccgg cgtcagcacg accacttccg ggtagtccag cggccgcggc gacaacgagg
      781 ccagcatgtc gaacagcagc gaggggtagt cgtccaccgg caggatgcgg ctgtgggcga
      841 acagctccgg aaacacctgc ttggtgacga tgcggttctc gagcatgtag gagacgccgg
      901 agggcacgcg gaggttgtct tccagcacgt agaccgtgcc gtcggcgtca cgcaccaggt
      961 cgctgccgca gatgtgcgcc catacgccgt gtgcgggctt cacacccatg cattcgcgca
     1021 ggaagttgcc cgaggtctcg atcagctcgc gcgggaagcg cccgtcgtcg agtatgcgct
     1081 gctcgttgta aatatcattg atgaacatgt tcagcgccgc aagtcgctgc ttcaggccgg
     1141 cctcgatgcg gcgccattcc tcggccggca tgacccgcgg gatgatgtcg aagggccagg
     1201 cccggtcgat gttctggcct tcgctgtaga ccgtgaaggt gatgcccatc tcgatgatgg
     1261 cgcgctcggc ggcctcctgg cgccggctga gctggctggg gctgagtgac gaaaggtact
     1321 tggtgagctg gcgcgcatgc gcgcggggct tgccgggccc cttgaggagt tcatcccagc
     1381 ccttgccggg atcgtactcg ctccagctga tcttcatcct ggcggccctc gcctgcggaa
     1441 cacgctacaa agcataacag cccggcgctg cagccccgcc gcgacctcag acatcaatgg
     1501 tgaagcgaat gtcggcatgt tgcgcgaggc gcccgatgag gcgcgcgccg aacgcggtgg
     1561 cgggcgtcca gaagccgccc gccacgggca gccggtcgcc gtccagcgcc aggcacatcg
     1621 cggcctcacc gagcatccgg gccgtggcgc cgtagcccgg gtcgcgccgc cccgtcaccc
     1681 gcgcctggat gtgacgtcgc ggagcggcgg gatgccggcc gaccagcagc atctcgaagc
     1741 cgccggcctc gcgcgccgcc tcgtcgggcc cctggccggg ctgcggcagg aacagcctgt
     1801 tcatcaccgc gcgcgccggg ccgacggagg ccgcggccat gaatccggcc agcccggcgg
     1861 tgagcgcggt ggcgcgcgcc agccccgcga cgccgtcgcc ggtgatgacc gactccgcat
     1921 agcggaaacg gcggccccat ggccagccca gcagcgcgtg gctgcgccgc acgatccgcg
     1981 tgttgaccgc cgccatcacg aacggtgcgg tccagccgtc ggcgtccggg tcgcgcagcg
     2041 gacgggcctg gtcgctgccg tccggcccgc tgcgctcacc cgcgggattg agcgcgtagg
     2101 gattgcccat gatgcgccgg tcctccgggc tgcgccgggc cgcctcgagc gcgttgagca
     2161 tgctggcgac ggtgccgccg ctgaaggtcc cttgcatgcg ccgcacgaac agtttcaccg
     2221 agctgcaggg acgaccgtag cgttcgatgg cgcgctgttg caggaaaagg cagcccagat
     2281 ccgagggaat ggaatcgaag ccgcaggcat gcaccagacg cgcgccgctt gccatggcgc
     2341 gggcctcgtg ggcatcgatc atgtggcgca tccagtgcac ttcgccgct
//
LOCUS       DAOMPG010000309         2384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17676_length_2384_cov_3.693860, whole genome shotgun sequence.
ACCESSION   DAOMPG010000309 DAOMPG010000000
VERSION     DAOMPG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17676_length_2384_cov_3.693860"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1106)
                     /gene="rlmJ"
                     /locus_tag="PLS34_12355"
     CDS             complement(249..1106)
                     /gene="rlmJ"
                     /locus_tag="PLS34_12355"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRP88291.1"
                     /translation="MNYRHAYHAGNFADVLKHAVMAAVLLRMQGRPKPLCYVESHAGA
                     GLYRLDSPEARRSREADNGILRLADTEPRSPALAAYLRLVAALPENDTALHAYPGSPL
                     LAAGLLDDDDRLVLVEAAPAEAEALGRLFRHDPRVAVHCRDGWEAIGALLPPTPRRGL
                     LLVDPPYEAPDDFRRVVAGLALARRRWNVGVLAAWYPIKDRRTLRPFYRALSAAGLGP
                     MLRIELAVRPDDNPAGLNGSGMVIAAPPFGLEQELRVLLDELSGALREPRAPRPRLDW
                     LTAPGEAGP"
     gene            complement(1103..2125)
                     /locus_tag="PLS34_12360"
     CDS             complement(1103..2125)
                     /locus_tag="PLS34_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88292.1"
                     /translation="MNDNEPDRVPDRVPVSGTRKGPGARLVVLYGAAVLFLLAALGLE
                     KPRSLIELTLRDLRDVFYLGFAALVILELTALVGRRWIRKRSLYYLVAVVAMLAMVFG
                     FELLADSDPEQPERWIRNLVGAFGFLALSAALDRGLKREHAWLRGWPRRSIGIAGALC
                     MLTALHPLVPVSASYAGRAGAFPVVVDLASLWQEPFIDVQGAELFVGKGPEEWSRRAG
                     RRVALLLFDGTPGGGVTVREPFKDWSGMSVMLINVYSTAPQPVRMILRLEDSSRLLPP
                     DERIDYALRIVPGANDFFIPLEALSTAPSGRSIDLTSIRRMGLYLGEPADPLRIYVHR
                     IRMSEQ"
     gene            complement(2171..>2384)
                     /locus_tag="PLS34_12365"
     CDS             complement(2171..>2384)
                     /locus_tag="PLS34_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88293.1"
                     /translation="VATISAEKSRSVRMAIEPGRVVLTVRNMEAGQAVEELEIDYSGE
                     PFELYFSPIWLEKDRGARIADGPRPD"
BASE COUNT          398 a          855 c          806 g          325 t
ORIGIN      
        1 gctcaggtcg atgaacaccc ggatgccggc gtccagcagc ccgacgatgc gccgtcgaac
       61 gatgctctcg tcccagtggc ccgggtgctc gcccgcgagc agccggccgg attcgaccca
      121 gtagcattcg ctgattggct tgagatccgt catcgcgccc ccttgatggc gagcagacta
      181 gcagcgcggg gactgtgcgg gcgtgacggg gttctcgttc aggagattat gtccgcttgg
      241 cagccatgtc acgggcccgc ctcgccaggg gccgtcaacc agtcgagtcg cggccgcggc
      301 gcgcgcggct cgcgcagggc gccggacaac tcgtccagca gcacgcgcaa ctcctgctcc
      361 aggccgaacg gcggcgccgc gatcaccatg ccgctgccgt tgagccctgc cggattgtcg
      421 tctgggcgga ctgccagttc gatgcgcagc atcggaccca gccccgcagc tgacagcgcg
      481 cggtagaaag gtcgcagggt cctgcgatcc ttgatcgggt accaggcagc cagcacgcca
      541 acgttccagc gccgcctggc cagcgcgagc ccggccacca cccgccggaa gtcatcgggc
      601 gcctcgtagg gcggatccac gagcagcagg ccgcgacgcg gcgtcggcgg cagcagtgcg
      661 ccgatggcct cccagccgtc gcggcagtgc accgcgaccc gcgggtcgtg acgaaacagc
      721 cgccccagcg cctcggcctc cgcgggtgcc gcttccacca gcaccaggcg gtcatcgtcg
      781 tccagcaatc cggccgccag cagcggcgag cccgggtagg cgtgcagcgc cgtgtcgttc
      841 tcgggcagcg cggcgaccag ccgaagatag gcggccaacg cggggctgcg aggctctgta
      901 tcggccagcc gcaggatccc gttgtcggcc tcgcgcgagc gccgcgcctc cggcgagtcg
      961 aggcggtaaa gcccggcgcc ggcgtggctc tcgacataac acagcggctt gggacggccc
     1021 tgcatgcgca gcagcaccgc tgccatcacg gcgtgcttca ggacatcagc gaaattcccg
     1081 gcgtggtagg cgtgccggta gttcattgtt cggacatgcg gatccggtgc acgtagatcc
     1141 gcagcgggtc ggccggctcg cccaggtaga gccccatccg cctgatgctg gtcagatcga
     1201 tacttcgacc ggacggcgcg gtcgacagcg cttcgagcgg aatgaagaaa tcgttcgcgc
     1261 cgggcacgat acgcaacgcg tagtcgatac gctcatccgg cggcagcagc cgcgaactgt
     1321 cctccagccg caggatcatc cgcaccggct gcggcgcagt tgaatagacg ttgatcagca
     1381 tgaccgacat gccgctccag tccttgaacg gctcacgcac cgtcacgccc ccgccggggg
     1441 taccgtcgaa cagcagcagg gccacccgcc ggcctgctcg tcgcgaccat tcctccgggc
     1501 ccttgccgac gaacagctcc gcgccctgca cgtcgatgaa cggctcctgc caaaggctgg
     1561 ccaggtcgac gaccacgggg aacgcgccgg cgcgaccggc ataggaggcg gacacgggca
     1621 cgagcgggtg cagcgcggtg agcatgcaaa gggcgccggc gatgccgatg gaacggcgcg
     1681 gccagccgcg cagccaggcg tgctcgcgct tgaggccgcg atcgagcgcg gcgctcagcg
     1741 caagaaaccc gaaggcgccg accaggttgc gaatccagcg ctccggctgc tcgggatcgc
     1801 tgtcggccag cagctcgaaa ccgaacacca tcgccagcat cgccacgacc gccaccaggt
     1861 aatacagcga acgcttccgg atccagcgcc ggccgaccag cgcggtcagc tcgaggatca
     1921 cgagcgcggc gaacccgagg tagaaaacgt cccgcaggtc gcgcagcgtg agttcgatca
     1981 ggctgcgcgg cttctccagg ccgagcgcgg cgagcaggaa cagcacggcg gcgccgtaca
     2041 gcacgacgag ccgcgcgccc ggtcccttgc gggtcccgga caccggcacc ctgtcgggca
     2101 cgcggtctgg ttcgttatcg ttcaagggtg gacattcccg gtcatcgcca agcggcggct
     2161 gcggcctacc ctaatccggc cgcgggccgt cggcaatcct cgcgccccgg tccttctcca
     2221 gccagatggg actgaaatac agctcgaacg gctcgccgga gtagtcgatc tccagctcct
     2281 ccaccgcctg gccggcctcc atgttgcgca cggtcagcac cacccggccc ggctcgatgg
     2341 ccatgcggac ggagcggctc ttctcggccg agatggtggc gacg
//
LOCUS       DAOMPG010000310         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17705_length_2381_cov_3.816853, whole genome shotgun sequence.
ACCESSION   DAOMPG010000310 DAOMPG010000000
VERSION     DAOMPG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17705_length_2381_cov_3.816853"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLS34_12370"
     CDS             complement(<1..253)
                     /locus_tag="PLS34_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRP88294.1"
                     /translation="MTAAKIVVPEAGARITIKDGVLQVPDNPIIPFIEGDGTGPDIWR
                     ATVRVLDAAVRKAYGGRKQIHWMEIYAGQKYNDLFNSWLP"
     gene            complement(320..1651)
                     /locus_tag="PLS34_12375"
     CDS             complement(320..1651)
                     /locus_tag="PLS34_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP88295.1"
                     /translation="MIPRTRAPKREIFGWAMFDFANQAYTLLIITVVFGDLFTRVIVG
                     SDGDDYRFGNFLWSLALSTSYLMVVVCSPIAGAIMDYTAAKKRFLFASYVLTVAATAA
                     LYFVAPGYALLGVLLLIVSNFAYSIGEAFIASFLPDLGPPQDLGKISGFGWALGYVGG
                     MASAVFVLLVLGEVSAENFERIRWVGPWAAVFFLVTAIPTFIWVRERGVPRRLPPGQG
                     YTAVGIARVRETLRDLKRQQDLATLLLSTFFAMSGIYIIITYAFIYGAQVIGWEEHIR
                     TLMFIIVQITAAVGALGFGFIQDRLGAGRTYRITLLMWMTAIALIYLTPQLAGWLSAL
                     MGRPVQAQHLFLVVGCVAGLSLGSCQSATRTLVGLFSPLSRAAEYFGFWGLSMKLAGV
                     VGLLGIGLLQAAFGLHRAVLFCILLFAAAFVISRRIDEARGRRIADSHHGA"
     gene            1792..2223
                     /locus_tag="PLS34_12380"
     CDS             1792..2223
                     /locus_tag="PLS34_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP88296.1"
                     /translation="MHTIRTILKNKKSGEVWSVGPDETVLEAITRMARLGIGALLVME
                     GERLVGIVSERDYARKIILQGRRSAETRVAEVMTREVATATEDMTAQQCLGMMTQGFF
                     RHLPVMDGERVIGLVSIGDLVKAVIADQQEVIEQLERYVTG"
BASE COUNT          458 a          763 c          809 g          351 t
ORIGIN      
        1 ccggcagcca ggaattgaac agatcgttgt acttctggcc ggcatagatc tccatccagt
       61 ggatctgttt ccggccgccg taggccttgc gcactgcggc gtccagcacg cgcaccgtgg
      121 cgcgccagat gtccggaccg gtgccgtcgc cctcgatgaa gggaatgatc ggattgtcgg
      181 gaacctgcag gacgccgtct ttgatcgtga tcctggcgcc ggcctcgggg acgacgattt
      241 tcgctgctgt catgtgggag actccttcgc gtggcgcgac cttctgcggg tcgactcgtt
      301 gaccgccgat gatacccgat caggccccgt ggtggctgtc cgcaatgcgc cggccgcgcg
      361 cttcatcgat gcgccggctg atcacgaagg cggctgcaaa caacaggatg cagaacagca
      421 ccgcgcggtg caggccgaag gccgcctgca acaggccgat gcccagcagg ccgaccaccc
      481 cggccagctt catggagagg ccccagaagc cgaagtactc cgcggcgcgc gacaggggcg
      541 agaacaggcc gaccagcgta cgggtggcgg actggcagga gcccaggctc agcccggcga
      601 cgcagcccac gaccaggaac aggtgctggg cctgcaccgg gcggcccatg agcgccgaca
      661 gccagccggc cagctgcggc gtcaggtaaa tcaacgcgat ggcggtcatc cacatgagca
      721 gggtgatccg gtaggtgcgc ccggcgccga ggcgatcctg gatgaagccg aagcccaacg
      781 cgccgaccgc ggcggtgatc tgcacgatga tgaacatcag ggtgcggatg tgttcctccc
      841 agccgatcac ctgcgcgcca tagatgaagg cataggtgat gatgatgtaa atgccgctca
      901 tggcgaaaaa agtggacaac aacagggtcg caagatcctg ttggcgtttc aggtcgcgca
      961 gggtttcacg gactcgcgca atgccgacgg ccgtgtagcc ctggccgggc ggcagcctgc
     1021 gcggcacgcc gcgctcgcgc acccagatga aggtcgggat ggcggtcaca aggaagaaca
     1081 ccgccgccca gggccccacc cagcgaatcc gctcgaagtt ttcagcgctc acctcgccga
     1141 gcaccagcag cacgaacacc gccgaggcca tcccgccgac gtagccgagg gcccagccga
     1201 agccggagat cttgcccagg tcctgcggcg gcccgaggtc cgggagaaag ctggcgatga
     1261 aggcctcgcc gatggaatag gcgaagttcg agacgatcag cagcagcacg ccgagcaagg
     1321 catagcccgg agccacgaag tacagcgctg ccgtggccgc cacggtgagt acgtagctgg
     1381 cgaacaggaa gcgcttcttg gcggcggtgt agtccatgat cgcgccggcg atcggcgagc
     1441 acaccaccac catcaggtag ctcgtggaga gcgccaggct ccagaggaaa ttgccaaacc
     1501 ggtagtcgtc gccgtcgctg ccgacgatga cgcgcgtgaa caggtcgccg aacaccaccg
     1561 tgatgatcag cagggtgtag gcctggttgg cgaagtcgaa catcgcccag ccgaagatct
     1621 cccgcttcgg ggcccgtgtg cgcgggatca tgcgcccgac gatcctctca gacatggcgg
     1681 caccatatca gcactcgggc ggctgcagca ggccccgggg cgccgccggg cctttgctcc
     1741 ggcccgcacc ttgcggtacc gtcaaccaga cggcttgctg gagggcccgc catgcatacc
     1801 atccggacga tcctcaagaa caagaaaagc ggcgaagtct ggtccgtagg tcccgacgag
     1861 acggtgctcg aggccatcac gcggatggcc aggctcggca tcggcgccct gctggtgatg
     1921 gagggcgagc ggcttgtcgg catcgtttcc gagcgcgact acgcccgcaa gatcattctg
     1981 cagggccgtc gttcggcaga gacgcgcgtg gcggaagtca tgactcgcga ggtggcgacg
     2041 gcgaccgagg acatgaccgc gcagcagtgc ctgggcatga tgacccaggg cttcttccgc
     2101 cacctgccgg tgatggacgg ggagcgcgtc atcgggctgg tgtcgatcgg cgacctcgtc
     2161 aaggccgtga tcgccgacca gcaggaagtc atcgagcagc tcgagcgcta cgtgacgggc
     2221 tgacggccag tgcgggactt gccatgaccg gtccgcgggg ccggccggga gacaaacatg
     2281 attgaagtcg gcggcctctt cggcctgatc atcctgatcc tcgacgtcta cgcgatcgtc
     2341 aaggtcatcc agagcaatgc cggcacgggc gtgaaggtcg c
//
LOCUS       DAOMPG010000311         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17742_length_2377_cov_4.863910, whole genome shotgun sequence.
ACCESSION   DAOMPG010000311 DAOMPG010000000
VERSION     DAOMPG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_2377_cov_4.863910"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLS34_12385"
     CDS             <1..956
                     /locus_tag="PLS34_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRP88297.1"
                     /translation="TFTMDALPGTPQIQNIIPTTFFGTNTWAAPVLGTIGGVFILILG
                     MSYLEWRRRAAAAKGEGYGDAASLANEPATYRGGALPNPVIAVLPLVLVGVANILFTW
                     WIPQAYGDSHSFIPAVIGSPAPVVQEVSRITAIWAVLGALLVGIGTVLIFAFRPVMSS
                     FAEGSKSAVGGALLAAGNTASEYGFGAVIAALPGFLVVANALGSIPDPLVNEAVTVTA
                     LAGITGSASGGMSIALAAMADTFIANANAAGIPMDVLHRVASMASGGMDTLPHNGAVI
                     TLLAVTGLSHRQSYKDIFAVTCIKTMAVFVIIGVYYATGLV"
     gene            1087..1938
                     /gene="ygiD"
                     /locus_tag="PLS34_12390"
     CDS             1087..1938
                     /gene="ygiD"
                     /locus_tag="PLS34_12390"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRP88298.1"
                     /translation="MAASPVLFIGHGNPMNTLETNRYTRAWRELGRRLPRPRAVLMVS
                     AHWYIGATAVTAMPWPRTIHDFYGFPQALFDFDYPAPGAPDLAAEVADVAKPDWVGLD
                     RDQWGLDHGAWSVLAHLYPEADVPVAQLSINALKPLEYHLQLAERLAALRERGVMIVG
                     SGNVVHNLRRVQWDKPEAATDWGERFDDAAIEQMASDPGAILKLAEHPDYGLAVPTPD
                     HFIPLLYAAGLAAAGEGRATAFARGHALGSISMTCYTVGCDAADTGHGSPPPALPAGV
                     PPEQTNL"
     gene            complement(2103..>2377)
                     /locus_tag="PLS34_12395"
     CDS             complement(2103..>2377)
                     /locus_tag="PLS34_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88299.1"
                     /translation="ATLALALIALVAASAFVFPGLCRMRHWRTLAICGALLGAALAIF
                     LTWPPQQLFPPLEGRHAAAQRLVLATYFGWFALAAGVALGRRREKT"
BASE COUNT          358 a          897 c          783 g          339 t
ORIGIN      
        1 tcaccttcac catggacgcc ctgcccggca ccccgcagat ccagaacatc atccctacca
       61 cgttcttcgg caccaacacc tgggctgcgc cggtgctggg cacgatcggc ggcgtcttca
      121 tcctgatcct gggcatgagc tatctggaat ggcgtcgccg cgccgcggcg gcgaaaggcg
      181 aaggctatgg cgatgccgcc agcctcgcga acgaaccggc cacctaccgg ggcggcgcgc
      241 tgcccaatcc ggtcatcgcg gtgctgccgc tggtgctggt gggcgtggcg aacattcttt
      301 tcacctggtg gattccgcag gcctacgggg acagccactc tttcatcccg gcggtgatcg
      361 gcagccccgc gccggtggtg caggaagtct cgcgcatcac cgcgatctgg gcggtgctgg
      421 gcgcgctgct ggtgggcatc ggcaccgtgc tgatcttcgc tttcaggccc gtcatgagca
      481 gctttgccga aggctcgaaa tccgccgtcg gtggtgccct gctcgcggcc ggcaacaccg
      541 cttccgaata cggattcggc gcggtgatcg ccgccctgcc cggcttcctc gtcgtcgcca
      601 acgcgctggg ctcgatcccc gatccgctgg tcaacgaggc cgtcaccgtc accgcgctgg
      661 ccggcatcac cggctcggcc tcgggcggca tgagcatcgc gctggcggcc atggccgaca
      721 ccttcatcgc caacgccaac gctgccggca tcccgatgga cgtgctgcac cgcgtcgcct
      781 cgatggcctc cggcggcatg gacaccctgc cccacaacgg tgccgtcatc acgcttctgg
      841 ccgtcaccgg cctctcgcac cgccagagct acaaggacat cttcgcggtc acctgcatca
      901 agaccatggc ggtgttcgtc atcatcggcg tgtactacgc caccgggctg gtctgaggaa
      961 cgcgccgctg tcctcacgcg gcgcgccttg tcgggccgaa gcacgctcgc catactcgcc
     1021 gcgtccggcc agccgcgcgg cccggatgcg cgcggcgcgc ccgcagaccc gcaggaggaa
     1081 ccggccatgg ccgcaagccc cgtcctgttc atcggccacg gcaatccgat gaacaccctg
     1141 gaaaccaacc gctacacccg ggcctggcgc gagctgggcc gccgcctgcc gcggccgcgc
     1201 gcggtgctga tggtgtccgc gcactggtac atcggcgcga ccgcggtgac ggcgatgccc
     1261 tggccacgca ccatccatga cttctacggc tttccgcagg ccctgttcga tttcgactac
     1321 cccgcgccgg gcgcacccga cctcgccgcc gaggtggcag acgttgccaa gcccgactgg
     1381 gtggggctgg atcgcgacca gtggggcctg gaccacggtg cctggagcgt gctcgcgcac
     1441 ctgtatcccg aggccgacgt gccggtggcg cagctttcga tcaacgcgct caagccgctc
     1501 gaataccacc tccagctcgc cgaaaggctc gccgccctgc gcgaacgcgg cgtgatgatc
     1561 gtcggcagcg gcaacgtggt gcacaacctg cgccgcgtgc agtgggacaa gccggaagcg
     1621 gccaccgact ggggcgagcg cttcgacgac gccgccatcg aacagatggc ctccgacccc
     1681 ggcgcgatcc tgaagctggc cgagcatccc gactacggcc tggcggtgcc gacgcccgac
     1741 cacttcatcc cgctgctcta cgccgccggg ctcgccgcgg caggcgaagg ccgcgccacc
     1801 gccttcgcac gcggccacgc cctggggtcg atctcgatga cctgctacac cgtcggctgc
     1861 gacgccgccg acaccggcca cggatcgccg ccgcccgccc tgcccgcggg cgtgccgccg
     1921 gagcagacca acctctgacc gcgcacgcaa acgccccgga gcgacaccgc gcctcccgcc
     1981 ggcgacagga tcgccgctgc gggcgaaagt ggcgaggtga atccggtggg tgcggccgag
     2041 cgcgctttcc tgctcgatgg aaggccccca gcggcgcaga ccgcagcccc gcgttaccgg
     2101 attcaagtct tctcgcgccg ccgccccaac gccaccccgg ccgccagtgc gaaccagccg
     2161 aagtaggtcg cgagcacgag ccgctgcgcg gcggcgtggc ggccttcgag cggggggaag
     2221 agctgctgcg gtggccaggt gaggaagatt gccagtgccg cgccgagcag cgcgccgcag
     2281 atggcgagcg ttcgccagtg ccgcatccgg cacaggcccg ggaaaacaaa ggccgacgcc
     2341 gccacgagtg cgatgagggc cagcgccagc gtcgcgg
//
LOCUS       DAOMPG010000312         2363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_17888_length_2363_cov_3.779463, whole genome shotgun sequence.
ACCESSION   DAOMPG010000312 DAOMPG010000000
VERSION     DAOMPG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17888_length_2363_cov_3.779463"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="PLS34_12400"
     CDS             complement(<1..1743)
                     /locus_tag="PLS34_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88300.1"
                     /translation="MNNTHSTIARRGTAPASYRRWWAAPLACLATLLALPVGAATDIP
                     DDPLTTGARVPPNVLFVLDDSGSMAFEAMPANAVSDTWTTRTYVHNAVYYNPAKTYLP
                     WVGADGTPLSGGTSYDAAYGSFNLVGGSTIDLGDSGSCGRFNHNQTPRGTDEFATGGT
                     LVCGGPQRFYVPKDPTSTNTSYLGNSSNYYMYKILRDGSGIVRGERGTVSESSTNLTI
                     SPGSGSVQQWNENTHTVTVPASATSASFSLTSSLRSVMYYVLDPNNIEICSGQVNEGN
                     TGTCHVPDPLAGTYQLIAWRWDRANSQANYTLSASSATGNRCGPGTGNTDWINCSAAL
                     PSASRSLSQELANYATWFSYHRTRMKAAKAGASAAFSELGTNIRVGFRTIWQQNGSVT
                     TGNWPRQAVPIPVQYNDGLFDDVTIDGDTHDNRSKWYRRLHGAIGQNGTPLHGALFQA
                     GTYFGSDAVTGPYGPQSGADQLACRQNFTILTTDGYWNNETQNYPSESRVGNVDGTDG
                     PTITEASTGASYTYEAVAPYADAHGETNGTLADVAMQFWKNDLRTGMRNIVPTTDANP
                     AFWQHMVTFGLSIGL"
     gene            complement(1747..>2363)
                     /locus_tag="PLS34_12405"
     CDS             complement(1747..>2363)
                     /locus_tag="PLS34_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HRP88301.1"
                     /translation="ADAHRLERGQHEEPDAMNHGLQRPLLAAPRRQSGISLVIVMVLL
                     IATSILGIAVLRSSAMQERMSANLRDRSLAFQAAEAALRYAQGRLAAGTWDITLPTSA
                     TCTSDSICPTGTGTGGSNPAIVWRVVPTADFNFTAAGLGAAPEYWIEYLGQGPGRRGS
                     CDTLPPSLDCRSPMYRVTARSRAEGRADVLLQANVISRIPDPGT"
BASE COUNT          388 a          718 c          812 g          445 t
ORIGIN      
        1 caggccgatg ctcaggccga aggtgaccat gtgctgccag aacgccgggt tggcgtccgt
       61 ggtcggcacg atgttgcgca tcccggtgcg gaggtcgttc ttccagaact gcatggcgac
      121 atcggccaag gtgccgttcg tttcgccgtg tgcatcggca tagggcgcaa ccgcctcata
      181 cgtgtaggag gcacctgtgc ttgcttccgt gatggtggga ccatccgtac cgtcgacgtt
      241 gcccacgcgg ctttccgacg gatagttctg ggtttcgttg ttccagtacc cgtccgtcgt
      301 caggatcgtg aagttctggc ggcaggcgag ctggtcggcg cctgactgcg ggccgtaggg
      361 cccggtcacc gcgtcactgc cgaagtaggt tccagcctgg aacagcgcgc cgtgcagggg
      421 cgtgccgttc tggccgatcg cgccgtgcag cctgcggtac cacttgctcc ggttgtcgtg
      481 cgtgtccccg tcgatggtca cgtcatcgaa caggccatcg ttgtactgca cgggaatcgg
      541 cacggcctgt cggggccagt tgcccgtggt gacgctgccg ttctgctgcc agatggtgcg
      601 gaagccgacg cggatattcg tgcccagttc gctgaaggcg gcgctcgcgc ctgccttcgc
      661 ggccttcatg cgtgtccggt ggtaggagaa ccaggtcgca tagttcgcga gctcctgaga
      721 caggctccgg gaagcgctcg gcaaagcggc actgcagttg atccagtcag tgtttcccgt
      781 acctggacca caccggttac cggtagcaga agacgccgaa agcgtgtaat tggcctggga
      841 gttcgcccga tcccatcgcc atgcaatgag ctggtaggtt cccgccaaag gatccggcac
      901 atggcaggta ccggtattgc cctcattcac ctgtccgcta cagatttcaa tgttgttggg
      961 atcaagcacg tagtacatca cgctacggag cgaggatgtg agagaaaaag acgccgaagt
     1021 cgcggaagcc ggaacagtga cggtatgggt attctcgttc cactgctgaa ccgaacccga
     1081 ccccggggag atcgtcagat ttgtcgagct ctcgcttaca gtcccgcgct caccgcgaac
     1141 aatgcctgag ccgtcacgca ggatcttgta catgtagtaa tttgaactat tgcctagata
     1201 gctcgtattg gtgctggtgg gatccttggg cacatagaac ctctgcgggc cgccgcaaac
     1261 cagcgtgccg ccagtcgcga attcatcggt accccgcggc gtctggttgt ggttgaaccg
     1321 tccgcaactg ccggaatcgc caaggtcgat cgtgctgcct ccgaccaggt tgaagctccc
     1381 gtatgctgcg tcgtacgacg tacctcccga cagcggagtg ccatccgcgc cgacccaggg
     1441 caggtaggtc ttggcaggat tgtagtagac ggcgttatgg acatatgtcc tcgtcgtcca
     1501 ggtgtcgctc acggcgttgg ccggcattgc ttcgaacgcc atcgaccccg agtcgtcgag
     1561 gacaaacaac acgttcggcg gcacgcgcgc acccgtcgtc agcgggtcat cgggaatgtc
     1621 ggtggcggcg ccgaccggca gcgccagcag tgtcgcgaga caggccagcg gggcggccca
     1681 ccagcgacgg taggatgcag gtgcggtgcc acggcgggcg atggtggaat gggtgttgtt
     1741 catggctcag gtccccggat ccgggatgcg gctgatgacg ttggcctgca gcaggacgtc
     1801 tgcacggcct tctgcccggc tgcgggcggt gacgcggtac atcgggctgc ggcagtcgag
     1861 cgagggcggc agggtgtcgc agctgccgcg tcgcccggga ccctgaccca ggtattcgat
     1921 ccagtactcc ggcgcggcgc cgaggccggc ggcggtgaaa ttgaaatccg ccgtcggcac
     1981 cacgcgccac acgatggctg gattggagcc gccggtcccg gtgccggtgg ggcagatgct
     2041 gtccgaggtg caggttgccg acgtgggcag cgtgatgtcc caggtgccgg ccgcgagccg
     2101 gccttgcgcg tagcgcagcg cggcctcggc ggcctggaag gccaggctgc ggtcgcgcag
     2161 gttggcgctc atccgctcct gcatggcgct gctgcgcagg accgcgatgc cgaggatcga
     2221 ggtggcgatc aggagcacca tcacgatgac cagcgagatg ccggactggc gccgcggcgc
     2281 cgccagcagg gggcgttgca agccgtggtt catagcgtcc ggctcctcat gctgaccacg
     2341 ttcgaggcgg tgcgcgtcag cgc
//
LOCUS       DAOMPG010000313         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18395_length_2311_cov_3.822695, whole genome shotgun sequence.
ACCESSION   DAOMPG010000313 DAOMPG010000000
VERSION     DAOMPG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18395_length_2311_cov_3.822695"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLS34_12410"
     CDS             <1..572
                     /locus_tag="PLS34_12410"
                     /inference="COORDINATES: protein motif:HMM:NF019643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HRP88302.1"
                     /translation="YAGTQAGLDKALAPLASLGPPLANQVGPVAYTALQRSGDISDPR
                     AEGLYLKGGFVMGLGPELVDALVDGFRPDPRRASIILFQAGGGAIARVPSAATAFAQR
                     DIFANMLVINGWRFGEDPTEHMQAARDHWQELERFTYGFYVNDLMPDATTAVIQANYR
                     QNHARLVEVKNKYDPTNLFRMNANIKPSV"
     gene            complement(646..1029)
                     /locus_tag="PLS34_12415"
     CDS             complement(646..1029)
                     /locus_tag="PLS34_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRP88303.1"
                     /translation="MTEPAIHLDTCFLIRALVPGTDADRRLRAWLTDGHRLRMDTIAW
                     TEFLCGPLPPEELRLAEQIVTERVSYSESDAQRAAELFNATGRRRGTLADCMIAAAAA
                     RRDAALATLNRGDFECFEASGLKLA"
     gene            complement(1022..1300)
                     /locus_tag="PLS34_12420"
     CDS             complement(1022..1300)
                     /locus_tag="PLS34_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRP88304.1"
                     /translation="MATPTIKSTYSLDPQTVRDLERLARKYGTSKSDILRRAVQSLAA
                     QDASPAAEGLLALEKLQTRIGLTAEKADAWVADVRNERAEMGRTAADD"
     gene            complement(1340..>2311)
                     /locus_tag="PLS34_12425"
     CDS             complement(1340..>2311)
                     /locus_tag="PLS34_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HRP88305.1"
                     /translation="QMEKFARLYRRYGENAAAMRQHRERSFTSWQEEDGMVSFRVRLP
                     AEQAAVVLQAIEAAKASLEGHDSEDDVSAESPPSPWDEPPEHPDEDVSAETRCARRAD
                     ALVRVAESYLEPDARPRSLGEKYQVHVHLDIRRNADGPVIDPEAPALAEDTIRRLGCE
                     AGLVPVLHDGDEILSVGRKTRAVPPAIRRALRLRDRGCRFPGCTHTRHVDAHHVRHWA
                     DGGETKLSNLVELCRGHHRLLHEGGYGLRVTDDGALVFTRPNGERIPDVPRPLVLTGN
                     PVAVLAADNQHLGVSAETFRCRWDGKPPDYGAMVELLDDKSRPAAGE"
BASE COUNT          361 a          824 c          771 g          355 t
ORIGIN      
        1 gctatgccgg cacccaggcc ggactggaca aggcgctcgc gccgctcgcc agcctcggcc
       61 cgccgctcgc aaaccaggtc ggcccggtgg cctacaccgc gctgcagcgc tccggcgaca
      121 tcagcgaccc gcgcgccgag gggctgtacc tgaagggcgg cttcgtcatg gggctcggcc
      181 ccgagctggt ggacgcgctc gtggacggct tccggccgga tccccggcgc gcatcgatca
      241 tcctgttcca ggccggtggc ggcgccatcg cgcgcgtgcc gtccgcggcc accgccttcg
      301 cgcagcggga catcttcgcc aacatgctgg tgatcaacgg ctggcgcttc ggcgaggatc
      361 ccaccgagca catgcaggcg gcccgcgacc actggcagga gctcgagcgc ttcacctacg
      421 gcttctacgt caacgacctg atgcccgacg ccaccaccgc ggtgatccag gcgaactacc
      481 gccagaacca cgcgcggctg gtcgaggtga agaacaagta cgacccgacc aacctgttcc
      541 ggatgaacgc caacatcaag ccgagcgtgt agcgcccggc tggtcgcgtt gagcgaggaa
      601 ctgaccggcg tggcggcgcg ctagccgatt aacgcccggg cgatttcacg ccagcttcag
      661 tccggatgct tcgaagcact cgaagtcacc ccggttgagc gttgcgaggg ccgcatcgcg
      721 tcgcgcggca gcggcagcga tcatgcagtc agccaacgta ccgcggcggc gcccggtggc
      781 attgaaaagc tcagcagcac gttgtgcatc ggactcgctg tagctgactc tctcggtcac
      841 gatctgctcg gcgaggcgga gctcctccgg tggcaggggc ccgcacagaa actcggtcca
      901 ggcgatggtg tccatgcgaa gccgatgacc atcggttaac caggcgcgca agcgacgatc
      961 cgcatccgtg cccggcacca gggcgcggat caggaagcat gtatcgaggt ggatcgcggg
     1021 ctcagtcatc ggccgcggtc cggcccatct cagcgcgctc gttgcggacg tccgcaaccc
     1081 aggcgtccgc cttttccgcg gtcagaccga tcctagtctg taacttctcc agcgcgagca
     1141 ggccctcggc tgccggagat gcgtcctgcg ccgccagcga ctgtactgcc cgccgcaaga
     1201 tgtccgactt cgacgtcccg tacttgcgcg ccaggcgctc caggtcccga accgtctgcg
     1261 gatcaagcga atacgtggat ttgatcgtcg gtgtggccat aagcaagata atatggccat
     1321 actcccgcgg gtgcaattgt cattccccgg ctgccggcct ggatttgtcg tccagcaact
     1381 cgaccatcgc cccgtaatcc ggcggcttgc cgtcccaccg gcagcggaac gtctccgcgg
     1441 agacgccgag gtgctgattg tccgccgcaa gcaccgccac cggattgcct gtcagcacca
     1501 gcgggcgcgg cacgtcgggg atgcgctcgc cgttggggcg cgtgaacacc agcgcgccat
     1561 catcggtgac gcgcagcccg taaccgccct cgtgcagcag ccggtgatgg ccgcggcaca
     1621 gctccaccag gttggagagc ttcgtctcgc cgccgtcggc ccagtggcgc acgtggtgcg
     1681 cgtcgacatg gcgagtgtgc gtgcagcccg ggaagcggca gccgcggtcg cgcagccgca
     1741 gggcccggcg gatcgccggc ggcacggcgc gggtcttgcg ccccaccgac aggatctcgt
     1801 cgccgtcgtg cagcaccggc accagcccgg cctcgcaacc cagccggcgg atggtgtcct
     1861 cggccagcgc gggcgcctca ggatcgatca ccggcccgtc cgcattgcgc cggatatcga
     1921 ggtgcacgtg cacctggtat ttctcgccga gggaacgcgg ccgggcatcc ggctcgaggt
     1981 aggactccgc cacccgcacc agggcgtcgg cccggcgggc gcaccgcgtc tccgcggaga
     2041 cgtcctcgtc gggatgctcg ggcggttcgt cccagggcga gggcggcgac tccgcggaga
     2101 cgtcatcctc gctgtcgtgg ccttccagcg aggccttcgc cgcttcgatg gcctgcaaga
     2161 ccaccgccgc ctgctcggcc ggcaggcgga cgcggaacga caccatgccg tcctcttcct
     2221 gccacgaagt gaaggagcgt tcgcggtgct ggcgcatggc cgcggcgttc tcgccatagc
     2281 ggcgatacag ccgggcgaat ttctccatct g
//
LOCUS       DAOMPG010000314         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18532_length_2297_cov_5.346566, whole genome shotgun sequence.
ACCESSION   DAOMPG010000314 DAOMPG010000000
VERSION     DAOMPG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18532_length_2297_cov_5.346566"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLS34_12430"
     CDS             complement(<1..193)
                     /locus_tag="PLS34_12430"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRP88306.1"
                     /translation="MELIDLTLIWVGLIGFGVFMYVLMDGFDLGVGILFPFAPDEESR
                     DLMMNSVAPVWDGNETWLIL"
     gene            complement(195..1601)
                     /locus_tag="PLS34_12435"
     CDS             complement(195..1601)
                     /locus_tag="PLS34_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRP88307.1"
                     /translation="MDLDPLLLSRVQFAFVVSFHAIFPVFSIGLAAYIAVLEGLYYKT
                     DDPVWLKLSKFWTKIFAIVFGMGVVTGIVMAFQFGTNWSNFSYAASNFLGPMLAYEVI
                     TAFFLEATFLGVLLFGREKVPRGLHLFAACMVSIGTCISSFWILAANSWMQTPAGVEL
                     VDGMIHMTSWTQAIFNSSFPYRFAHFLLASFITGGFAVAGVSAWYLLRGRHADIAEKA
                     LKMCVIMLAVATPLQLLSGDMHGLNTLEEQPVKVAAMEGNWDTRGNVPLLLFAIPDAA
                     SETNHFEIGIPSGASLILKHSADGVVPGLKSVPPEERPPVGIVFWSFRVMVGIGTLMI
                     AVSLWGAWLLYRRRLYRSTLLLRIFTLMIPLPFVAVLAGWFVTEIGRQPWIIYGLMTV
                     SEGVTPSLSGWMVLATLVGYVTVYAVVFAAGIHYLRKVIKAGPDPVGDAHERITGTPK
                     RPWSAVEGEFEAAGAARS"
     gene            1863..2135
                     /locus_tag="PLS34_12440"
     CDS             1863..2135
                     /locus_tag="PLS34_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRP88308.1"
                     /translation="MNKGELIEAVAAAADLTKADATKAVEAFVDTVTRALRKGDQVAI
                     VGFGSFSVKSRAARQGRNPKTGATIDIPASRVPGFKAGKALKDAVN"
     gene            2156..2231
                     /locus_tag="PLS34_12445"
     tRNA            2156..2231
                     /locus_tag="PLS34_12445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2189..2191,aa:Val,seq:tac)
BASE COUNT          456 a          736 c          753 g          352 t
ORIGIN      
        1 cgaggatcag ccaggtctcg ttgccgtccc agaccggggc gaccgagttc atcatgagat
       61 cgcgcgattc ctcgtcgggg gcgaagggaa acaggatgcc gacgccgaga tcgaagccat
      121 ccatgagcac gtacatgaac acgccgaagc cgatcagccc cacccagatg agcgtcaggt
      181 cgatgagttc catgtcagct cctcgccgcg ccggcggcct cgaactcgcc ctcgaccgcg
      241 gaccagggcc gcttgggcgt gccggtgatg cgttcgtgcg cgtccccgac cgggtctggt
      301 ccggccttga tcaccttgcg caggtaatgg atgccggcgg cgaacacgac ggcgtagacg
      361 gtcacgtagc cgaccagcgt ggcgagcacc atccagccgc tcagcgacgg cgtgacgcct
      421 tcgctgaccg tcatcaggcc gtagatgatc cacggctggc gcccgatctc ggtgacgaac
      481 cagccggcca gcaccgcgac gaacggcagc ggaatcatca gggtgaagat cctgagcagc
      541 agcgtcgacc ggtagagcct gcgccggtac agcagccagg cgccccacag cgacaccgcg
      601 atcatcagcg tgccgatgcc caccatgacg cggaaactcc agaacacgat accgaccggc
      661 ggccgctcct ccggcggcac cgatttcagg cccggcacca cgccgtcggc gctgtgcttg
      721 aggatcaggc tggcgccgct ggggatgccg atctcgaagt ggttcgtctc gctggcggcg
      781 tccgggatgg cgaacagcag cagcggcacg ttgccacgcg tgtcccagtt gccctccatc
      841 gccgccacct tgaccggctg ctcctccagg gtgttcagcc cgtgcatgtc cccggacagc
      901 agctgcagcg gcgtggccac ggccagcatg atgacgcaca tcttcagcgc cttctcggcg
      961 atgtcggcat gccggccgcg cagcaggtac caggcgctca cgcccgctac cgcgaagccg
     1021 ccggtgatga aggaggccag caggaagtgt gcgaagcgat aggggaagga ggagttgaag
     1081 atggcctggg tccacgaggt catgtggatc atgccgtcca ccagctccac gcccgccggc
     1141 gtctgcatcc agctgttggc ggccaggatc cagaacgagg agatgcaggt gccgatcgac
     1201 accatgcagg cggcgaacaa gtgcaggccg cgcggcactt tctcgcggcc gaacagcagc
     1261 acgccgagga aggtggcctc gaggaaaaag gcggtgatga cctcgtaggc cagcatcggg
     1321 ccgaggaagt tcgaggcggc gtaggagaag ttgctccagt tggtgccgaa ctggaacgcc
     1381 atgacgatcc ccgtgaccac gcccatgccg aacacgatgg cgaaaatctt ggtccagaac
     1441 ttggagagct tcagccacac cgggtcgtcg gtcttgtagt aaagcccttc cagcaccgcg
     1501 atgtaggcgg ccagaccgat ggagaacacc gggaagatgg cgtggaagga cactacgaag
     1561 gcaaactgga cacgcgaaag caatagcggg tcgaggtcca tgagtccgtc ccctttctgg
     1621 aatgggtcca tgatacggaa aaacggcggg tgaccgaagg gtgtcggttt caacagtgcc
     1681 gttgcggtga cttgtggcgt cgcgacatgc cgcggcggca gcgctttgat tgacctcacc
     1741 gggcgcactt gctataaaac tcgccctctc acggcgcact cccgctcggc tcgccgcttc
     1801 ggcgggctcc gcatctcgaa gccgggagac aacacccatc tacagaacac gaaggtacgc
     1861 tgatgaacaa gggcgaattg atcgaggccg tggctgccgc cgccgacctc acgaaggccg
     1921 atgccaccaa ggccgtcgag gcatttgtgg acaccgtcac gcgcgcgctg aggaagggcg
     1981 accaggtcgc cattgtcggc ttcggcagct tctccgttaa gagccgcgcc gcgcgccagg
     2041 ggcgcaaccc gaagaccggc gcaaccatcg acattcccgc cagtcgagtc cccggtttca
     2101 aggctggcaa agccctgaag gacgctgtaa actagcgcgc cctcccgggg agcgcgggtg
     2161 cttagctcag ctgggagagc gtcgccctta caaggcgaag gtcgaaggtt cgatcccttc
     2221 agcacccacc aggttgactt caggagcggt agttcagttg gttagaatac cggcctgtca
     2281 cgccgggggt cgcgggt
//
LOCUS       DAOMPG010000315         2296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18548_length_2296_cov_2.472557, whole genome shotgun sequence.
ACCESSION   DAOMPG010000315 DAOMPG010000000
VERSION     DAOMPG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_2296_cov_2.472557"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLS34_12450"
     CDS             complement(<1..573)
                     /locus_tag="PLS34_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88309.1"
                     /translation="MSEPRDDLAILVVTEILGDAELVQRLLSFEFGNVRISTDPLRCV
                     EEFEAAAPEVLVLAFNSLEKAEEYHLELQAQSALARRGLHRTLVLCTREEVRHAYDLC
                     KLGMFDDYVLFWPLTHDTPRLLLAVRHAMKHLPARGAPAADAQAEEPVERADEPAVPA
                     GQDTGGTDTATRRPLVLVVGAIREALAKAGV"
     gene            complement(570..>876)
                     /locus_tag="PLS34_12455"
     CDS             complement(570..>876)
                     /locus_tag="PLS34_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRP88310.1"
                     /translation="DDDTIVRGLLGDYLASAGAEVEKVEAAIAAADARAIAFAAHKLK
                     SSSRSVGAVPFGELCARLESVAKAGDCAAAAGLGDDFSRQWAAVRDAVSAALGKAAP"
     assembly_gap    877..890
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<891..>2296)
                     /locus_tag="PLS34_12460"
     CDS             complement(<891..>2296)
                     /locus_tag="PLS34_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP88311.1"
                     /translation="IADILDFSKIEAGRLEVEHAPVSVRDLVEGLCDSLVPVAASQNV
                     QLSLFVSPDVPEQVHSDEVRIRQVLYNIVGNGIKFSRGRPERRGRVSVRVEHRDGYPA
                     RLDITIRDNGVGMGREVVDRLFMPFSQAEVSTTRRFGGTGLGLTICKRLVGLMGGEIG
                     VSSEPGRGSTFTVSLPVDVPETQPGRTLPDLSGLSCVLVDSDAYDMQDLSVYLRHAGA
                     GVQRAASPAAAHALGAAAAGSAVIIDYLGTQPSAQVMDECEPPCGWLHITSGRPSGPE
                     IDGRHVYLDETSQRMKSLLDAVALAGGRVPAGEAHAIMRPPEPVAPAQPQAPQRDVRI
                     LVAEDDEISSKVVLQQLERLGYPAQRVANGSEALEKWRTGGFGLVLTDLHMPEMDGYS
                     LARQIRSAEAGLAKPRSGPARVPIIALTANALRDEPERARAAGIDEYLTKPIELQHLS
                     EVLSRWLPDSPVGGTLEL"
BASE COUNT          350 a          822 c          771 g          339 t
ORIGIN      
        1 aacgccggcc ttggccagcg cttcgcgaat tgcgccgacg accagcacca gcgggcgtcg
       61 cgtcgcagta tccgtcccgc cggtatcctg gccggccggc acggcgggct catcggcgcg
      121 ctcgacgggc tcctccgcct gggcgtcagc ggccggcgcg ccgcgcgccg gcaagtgctt
      181 catcgcatgc cgcacggcca gcagcaggcg gggcgtgtcg tgggtcagcg gccagaacag
      241 cacgtagtcg tcgaacatgc ccagcttgca gagatcgtat gcatgacgca cttcctcgcg
      301 ggtgcacaac accagggtac ggtgcagtcc acgacgcgcc agggccgact gcgcctgcag
      361 ttccagatgg tattcctcgg ccttctcgag gctgttgaag gcgaggacca ggacctcggg
      421 cgcagccgcc tcgaattcct cgacgcagcg caacgggtcg gtcgagatgc gcacgttgcc
      481 gaactcgaag gacagcaggc gctgcaccag ctccgcgtca ccgagaatct cggtgaccac
      541 caggatggcg aggtcgtcgc gcggctcgct catggcgcgg ccttccccag ggccgccgac
      601 acggcatctc gcaccgcagc ccactggcgc gagaaatcgt cgcccagtcc ggcggccgcc
      661 gcacaatcgc ccgccttggc gaccgactcg aggcgggcgc acagctcgcc gaaggggacc
      721 gcgcccaccg agcgcgacga cgatttcagc ttgtgggcgg cgaaggcgat cgcgcgcgca
      781 tccgcggccg cgatggccgc ctcgactttt tcgacctcgg cgccggcgga ggcaaggtaa
      841 tcgcccagca gcccccgcac gatcgtgtcg tcgtccnnnn nnnnnnnnnn tggagttcca
      901 gtgtcccgcc gaccggcgaa tccggcagcc atctggagag gacctcgctc aggtgctgca
      961 gctcgatcgg cttcgtcagg tactcgtcga tcccggcggc gcgggccctt tccggctcgt
     1021 cgcgcagcgc gttcgcggtc agggcgatga tcggcactcg agccgggccg ctccggggtt
     1081 tggccaggcc ggcttccgcg ctgcggatct gtcgcgccag gctgtagccg tccatctccg
     1141 gcatgtgcag gtcggtcagc acgagcccga aaccaccggt gcgccatttc tccagcgcct
     1201 cgctgccgtt cgccacacgc tgtgcggggt aacccaggcg ctcgagctgc tgcagtacga
     1261 ccttgctgct gatctcgtcg tcttccgcca ccaggatccg aacgtctcgt tgcggcgctt
     1321 gcggctgcgc cggcgccacc ggctcggggg gacgcatgat cgcgtgggct tcacccgccg
     1381 gcacgcgccc gccggccagc gccacggcgt ccagcagcga cttcatgcgc tgggacgtct
     1441 cgtcgagata cacgtgacga ccgtcgatct ccgggcccga cgggcgcccg ctggtgatgt
     1501 gcagccaacc acagggcggc tcacattcat ccatcacctg cgccgacggc tgcgtgccga
     1561 gatagtcgat gatcacggcg gagcccgccg cggccgcgcc gagcgcatgc gccgccgcgg
     1621 gcgacgctgc gcgctgcacg cccgcgccgg catgccgcag gtagacggac aagtcctgca
     1681 tgtcgtaggc gtcgctgtcg accagcacgc aggacagccc ggacagatcg ggcagcgtcc
     1741 tgccgggctg ggtttccggc acgtcgaccg gcaggctgac ggtgaaagtg gagcccctgc
     1801 ccggctcgct gctcacgccg atctcaccgc ccatcaggcc gacaaggcgc ttgcagatgg
     1861 tcagtcccag cccggtgccg ccgaagcggc gcgtggtaga gacttccgcc tgcgagaagg
     1921 gcatgaacag gcggtcgacc acttcgcggc ccatgccgac accgttgtct cggatggtga
     1981 tatcgagccg cgcgggatag ccgtcgcggt gctcgacgcg caccgagacg cgaccacgcc
     2041 gctccgggcg gccgcgcgag aacttgatgc cgttgccgac gatgttgtag agcacctggc
     2101 gtatgcgcac ctcgtcggag tgcacctgct ctggcacgtc cggcgacacg aacagggaca
     2161 gctgcacgtt ctgcgaggcg gccacgggca ccagcgagtc gcacaacccc tcgaccaggt
     2221 cgcggacgga gaccggtgca tgctccacct caagccggcc cgcctcgatc ttcgagaagt
     2281 ccaggatgtc ggcgat
//
LOCUS       DAOMPG010000316         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18569_length_2294_cov_3.946405, whole genome shotgun sequence.
ACCESSION   DAOMPG010000316 DAOMPG010000000
VERSION     DAOMPG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18569_length_2294_cov_3.946405"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLS34_12465"
     CDS             complement(<1..461)
                     /locus_tag="PLS34_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRP88312.1"
                     /translation="MPANTPANAKPVGATDWRGWVRRIALFAALWWILTDGVVSSWLI
                     GAPTVLLAAWLSRSLWADPPLSLSGVARFLPYFAWQSLVGATDVARRALQPRMPLYPG
                     LVRHRLRLPPGVSRVSLANVVSMLPGTLSADIEGDELVIHALDARSDLHAMV"
     gene            complement(491..1231)
                     /locus_tag="PLS34_12470"
     CDS             complement(491..1231)
                     /locus_tag="PLS34_12470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP88313.1"
                     /translation="MSFAYREDLSWIHDLGFGDLARNAGELLLRTLAADGIGEGLVVD
                     LGCGTGILAEAVSNAGYDVLGVDLSPAMIEMARRRVPEGRFVCESLLAAELPPCIAVA
                     AVGECFNYRFDESNSRDQLRALFGRIHAALAPRGVLLFDAAGPGRIAGEQPLKTHVDG
                     GDWALLMNAVEDREARRVTRCITSFRRVGDYFRRDDECHELDLLPAEELVDDLEAAGF
                     RAQVLDEYAGHGLPVGLSGFLARPVLRP"
     gene            complement(1368..>2294)
                     /locus_tag="PLS34_12475"
     CDS             complement(1368..>2294)
                     /locus_tag="PLS34_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88314.1"
                     /translation="GLSERHQRLQARSGGLLGGVLLAIFDGITRVYGRIQQRVAWSLR
                     SGRRQFLLGLGDAGRARRRLVETLLVGLPGSPEQVFLEAAWGTIEQERLETVRAALAR
                     VRRGAGQAGALDERRQEARVGATHSLAGLMAGAVFDARLDWLGARARDEGVEAARAGL
                     AALHREFAATRTRLSTVVVELAEARSRGEDITREASRTIREVLEREARALAAAAATSS
                     IEAAPGPAPPRPVRRRREAFSAWLLDLGRLAVAELRQHPSSWPQSESLDTEPTVVSTR
                     RGIQRYARDVALNIVAIFLVMLLLAAVTWLGA"
BASE COUNT          380 a          864 c          763 g          287 t
ORIGIN      
        1 accatggcgt gcaggtcgct gcgcgcatcg agcgcgtgaa tcaccagctc gtcgccctcg
       61 atatcagcgc tcagcgtgcc cggcagcatg ctgaccacgt tggcgagaga gactcgtgaa
      121 acaccgggcg gcaagcgcag ccgatgacgc accaggccgg gatacagcgg catcctgggc
      181 tgcagggcgc ggcgcgccac gtccgtcgca ccgaccagcg actgccacgc aaaatacggc
      241 aggaagcggg ccactccgct cagggacagc ggcggatcag cccacagcga gcggctgagc
      301 caggccgcca gcaacaccgt cggcgcgccg atcagccagc tcgataccac gccatccgta
      361 agaatccacc agagtgccgc gaacaacgcg atgcgccgca cccagccgcg ccagtcggtc
      421 gcaccgacgg gcttggcatt ggcaggcgtg ttcgcaggca tcattgctcc tttacgatac
      481 cgggctccgt tcagggacgc aggacgggcc gcgccaggaa tccggacaag ccgacgggca
      541 gcccatgccc ggcgtattcg tccaggactt gtgcccgaaa gcctgccgcc tcgaggtcgt
      601 cgaccagttc ctctgccggc agcaagtcca gctcgtgaca ctcgtcgtca cggcggaagt
      661 agtcacccac gcgccggaag ctggtgatgc agcgcgtgac gcggcgcgcc tcccggtcct
      721 ccaccgcgtt catcagcaag gcccagtcgc cgccatcgac atgagtcttc aggggctgct
      781 ccccggcgat tcgcccgggg ccggcggcgt cgaacagcag cacgccacgc ggcgccaacg
      841 ccgcgtgaat acggccgaac agcgcgcgca actggtcccg cgagttgctc tcgtcgaagc
      901 ggtaattgaa gcactcgccc accgccgcga cggcgatgca cggcggcaac tccgccgcca
      961 gcagcgattc gcacacgaac cgcccctccg ggacccgccg gcgggccatt tcgatcatgg
     1021 cgggcgagag gtccacgccc agcacgtcat agccggcgtt cgacaccgcc tcggcgagaa
     1081 tccccgtgcc gcaaccgagg tccaccacca ggccctcacc gatgccgtcc gcggcgagcg
     1141 ttcgcagcag caactcaccc gcgttgcggg ccaggtcgcc aaaaccgaga tcgtggatcc
     1201 aggacaggtc ttcccggtag gcaaagctca tggcgatgct ccttccctgg caggcaccgg
     1261 caaagcgcgg gcacggaatc attccgctgc gtgcggcacg gccggatgac cataatggac
     1321 tgtggcaagc ccttgccgcg ttggggtttt ccgaagtggc gcggcactca ggcgccgagc
     1381 caggtcactg ccgccaacag gagcatgacc aggaagatgg ccacgatgtt gagtgcgacg
     1441 tcgcgggcgt agcgctggat gccgcggcgg gtcgacacca cggtcggctc ggtatcgagc
     1501 gactcggatt gcggccacga ggacgggtgc tggcgcagct cggcgaccgc cagccggccg
     1561 aggtccagca accatgcact gaaggcttcg cggcggcgcc gcaccgggcg cggcggcgcc
     1621 gggccgggcg cggcctcgat cgacgacgtc gcggcggccg cggcgagcgc ccgtgcctcc
     1681 cgctccagca cctcgcggat ggtgcgcgac gcctcccgcg taatgtcctc gccgcggcta
     1741 cgggcctccg ccagctcgac caccaccgtg ctgagtcggg tccgggtcgc cgcgaattcc
     1801 ctgtgcaacg cggccagtcc ggcccgcgcg gcctcgacgc cctcgtcgcg cgcccgcgcg
     1861 cccagccagt ccaggcgggc gtcaaacact gcacccgcca tcagcccggc cagcgagtgg
     1921 gtcgccccga ccctggcctc ctgccggcgc tcatccaggg cgccggcctg gcccgcgccg
     1981 cgccgcacgc gcgccagagc cgcacggacg gtctccagcc gctcctgctc gatcgtgccc
     2041 caggccgcct cgaggaagac ttgttccggc gaccctggca gcccgaccag cagggtctcg
     2101 accaggcggc gacgcgcccg gcccgcatcg ccgaggccga gcaggaactg tctccggccg
     2161 ctgcgcaacg accaggcaac gcgctgctgg atgcgcccgt agacccgcgt gatgccgtcg
     2221 aagattgcca gcagcacgcc gccgagcagg ccgccgctgc gcgcctgcag gcgctggtgg
     2281 cgctccgaca ggcc
//
LOCUS       DAOMPG010000317         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18609_length_2289_cov_3.118174, whole genome shotgun sequence.
ACCESSION   DAOMPG010000317 DAOMPG010000000
VERSION     DAOMPG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18609_length_2289_cov_3.118174"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLS34_12480"
     CDS             <1..262
                     /locus_tag="PLS34_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017998484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HRP88315.1"
                     /translation="VVNPLVFAGAPRLHRGGLASDEVPTILQRGEEVLTRSDPRHVLN
                     AGMAGGNRTEINVSGTSDPALVITAAVQAADIIERRQARRRR"
     gene            263..862
                     /locus_tag="PLS34_12485"
     CDS             263..862
                     /locus_tag="PLS34_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88316.1"
                     /translation="MALITFPEGLRPQTATYDLIRADAGEDGPFGELQVIERLHTAKW
                     MQVLGFTALKGDLRRLALGFAQHIGFRGNTVRLPIFGYERGGPGGGTPLVAGASQTGR
                     TLATDGWPNSTLVLRYGDLIEVNGGLHQLTADATTNGTGQVTLQIEPALKSSPADNAP
                     IEYLQPTALFQFQGGQMARVTRIDEGQVTSDLTIPLLEA"
     gene            863..1510
                     /locus_tag="PLS34_12490"
     CDS             863..1510
                     /locus_tag="PLS34_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88317.1"
                     /translation="MPRDLESGGQAAIEARFVSMLVFARFDFASGVIRVHNGGAIVPL
                     GGHDWLPLGSHGTLEALDETLDVRGRSIQFSLTGLDSDLKAAVTSTSEADYYGRECEV
                     YVAFFNTETCAPLTGLISLGWAYMDEMDAEISRGQARIELRADSEQARWDKASGWRLN
                     HEHQQLLYPGDMGFEYQHTEGDAVWGKERVASRGGGYGIFPNPWDNDLIDWLDGR"
     gene            1507..1911
                     /locus_tag="PLS34_12495"
     CDS             1507..1911
                     /locus_tag="PLS34_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88318.1"
                     /translation="MNRAQVPEILAGFNGLRWRWGVLDCCMFAAAVLEQLHGRDFAAG
                     WRGKYRTHARAQRLIEERGGLEAILTEIFGPPVPPLTVRRGDPVLARDPDRFHDGEAV
                     GIADGRLAVFITSDGLMTMPLSRCAMGWRVDG"
     gene            1904..>2289
                     /locus_tag="PLS34_12500"
     CDS             1904..>2289
                     /locus_tag="PLS34_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88319.1"
                     /translation="MGDGVKKLVGAALTIGSFFVPGGQLIRAATMLAGTSLLSSAFAP
                     RMPRATFRNEFRVGPVQSMESPEPAFYGTVRTSGTFAFPPRVSGANSEYLWYCVVFGR
                     KGEADVAVTSYEKLYIDGTEIDVATE"
BASE COUNT          367 a          748 c          810 g          364 t
ORIGIN      
        1 ggtggtcaac ccgctggtgt tcgccggcgc accccggctg catcgcggcg gactggcctc
       61 ggacgaagtg ccgaccatcc tgcagcgcgg cgaggaagtg ctgacccgct cggatccgcg
      121 ccatgtgctg aatgccggca tggccggcgg caaccgcacg gagatcaacg tctcgggcac
      181 cagcgacccg gccctggtca tcacggctgc agtgcaggcg gcggacatca tcgagcgccg
      241 gcaggcgcgg cggaggcggt aagtggcgct catcacgttt cccgaaggct tgcgcccaca
      301 gaccgcgacc tatgacctga tccgtgcaga cgccggagag gacgggccct tcggcgagct
      361 gcaggtgatc gaacggctgc ataccgccaa gtggatgcag gtgcttgggt tcaccgcact
      421 gaagggcgac ctgcgccgcc tggcgctggg cttcgcgcag cacatcgggt tccggggcaa
      481 caccgtgcgg ctgccgatct tcggctatga gcgcggcggg ccggggggcg ggactccgct
      541 ggtggccggc gccagccaga ccggccggac gctggcgacg gacggctggc cgaacagcac
      601 gctggtcctg cgctacggag atctgatcga ggtcaatggc gggctgcacc agctcaccgc
      661 cgacgccacc acgaacggca ccggccaggt gacgctgcag atcgagccgg ccctcaaatc
      721 atcccctgcg gacaacgcgc ccatcgagta cctgcagccc accgcgcttt tccagttcca
      781 gggcggacag atggctcgcg tgacccgcat cgacgagggg caggtgacaa gcgacctcac
      841 catccccctg ttggaggcat agatgccccg cgatcttgag tccggcggcc aggccgccat
      901 cgaggcgcgc ttcgtctcga tgctggtatt cgcccgtttc gattttgcgt ccggtgtgat
      961 ccgggtccat aacggcggcg cgattgtccc gcttggtggc cacgactggc ttccgctcgg
     1021 ctcgcacggc acgctcgagg cgctcgacga gacgctggac gtgcgcggcc ggtcgatcca
     1081 gttctcgctt accgggctgg acagcgacct caaggccgca gtgaccagca cctccgaggc
     1141 cgactactac ggccgcgagt gcgaggtcta tgtcgcgttc ttcaacaccg agacctgcgc
     1201 gccgctgacc ggattgatct cgctcggctg ggcgtacatg gacgagatgg acgcggagat
     1261 ctcccgcggc caggcgcgca tcgagctgcg ggcggattcg gagcaggcac gctgggacaa
     1321 ggcatcgggc tggcgattga atcacgagca ccagcagctg ctctacccgg gcgacatggg
     1381 cttcgagtat cagcacactg agggcgatgc ggtgtggggg aaggagcgcg tagcctctcg
     1441 tggaggcggt tacgggatat tcccgaaccc gtgggacaac gatctcattg actggctgga
     1501 cggaagatga accgcgcgca ggtgccagaa atactggccg gtttcaacgg cctgcgatgg
     1561 cgctggggcg tgctcgactg ctgcatgttc gctgcggcag tgctcgagca gctgcatggc
     1621 cgggacttcg cggccggctg gcgcggcaag taccgcaccc acgcccgcgc tcagcggttg
     1681 atcgaggagc gcggcggact ggaggcgatc ctgacggaga tcttcgggcc gcccgtgccg
     1741 ccgttgacag tgcgccgcgg cgacccggtg ctggcgcgcg acccagacag gtttcacgac
     1801 ggcgaggcgg tcggcattgc ggacggccgg ctggcggtat tcataacgag cgacggcctg
     1861 atgaccatgc cgctgtcgcg ctgcgcgatg ggatggcggg tcgatgggtg acggcgtcaa
     1921 gaaactcgtc ggcgctgcgc tgacgatcgg cagctttttc gtccccggcg gccagctcat
     1981 ccgggcggcg accatgctcg ccggcaccag cctgctgtcg tccgcgttcg cgccccggat
     2041 gccccgggcg accttccgaa atgagttccg cgtcggcccg gtccagtcga tggaatcgcc
     2101 ggagcccgcg ttctacggca cggtgcggac ctccgggacg ttcgcgttcc cgccgcgggt
     2161 gtccggcgca aacagcgagt atctctggta ctgcgttgtg ttcggccgca agggcgaggc
     2221 tgacgtcgcc gtcaccagct acgagaagct gtacatcgac gggaccgaga tcgacgtggc
     2281 gacggagat
//
LOCUS       DAOMPG010000318         2264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_18849_length_2264_cov_3.150294, whole genome shotgun sequence.
ACCESSION   DAOMPG010000318 DAOMPG010000000
VERSION     DAOMPG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18849_length_2264_cov_3.150294"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLS34_12505"
     CDS             complement(<1..649)
                     /locus_tag="PLS34_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRP88320.1"
                     /translation="MRPEWSQAVALVGLLALLLGLLIAWFGTRRLRAGQRMVPAGGRP
                     AEPGEPAVDATRWTYQFLEQVLDRVQDGVIVCDEHGRVIEANEAAARLLQRDAHTLAG
                     KDLHDLAEPSVFDNRDALLPEGSTRLKTPTDEATAVAFTSCLLVEPDGLRRWVLVIRN
                     LSEAHRTQKRIRYLARYDTLTRVPNRMEFQHRLQQAIARARRTTTRIALLYVDLDG"
     gene            complement(714..1100)
                     /locus_tag="PLS34_12510"
     CDS             complement(714..1100)
                     /locus_tag="PLS34_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP88321.1"
                     /translation="MKNRRMRDRIGPPATILGPGARLNGSLQGEGHFLIAGQVTGDAD
                     IEGGLTLAEGGRWQGAIRADDVILAGELDGELLARGRVEITASARVRGRVVGNGISIS
                     QGAVVEADLQSMGDTDVVSFDERRKG"
     gene            1196..2068
                     /locus_tag="PLS34_12515"
     CDS             1196..2068
                     /locus_tag="PLS34_12515"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HRP88322.1"
                     /translation="MPRFRAGKFLLALLVLAALGAGSGCATSFLYERADRFANRWMGD
                     YLELDPAQQESLDTALAELHRWHRREQLPAYAAWLRSAAADLAAGGPYAERALRGRGT
                     ELGGFWRELAEAGAPALTAVGATLSDAQVEVLLEALREARSKEFEYAAARPDSYHQQR
                     SARSMERFLRRWTGRMTAAQQAMIRDWAAALEPSRVASLENRAGWIEALERALERRAD
                     PAALRQAAESLFVAPSSRWDPDYAALVERNTVHTTTFMAEFLAGLDERQRRRAVERLE
                     RLAAEFEQLAAAGS"
BASE COUNT          325 a          795 c          794 g          350 t
ORIGIN      
        1 agccgtccag gtcgacgtag agcagggcaa tgcgcgtggt ggtgcgccgc gcccgggcga
       61 tcgcctgttg caggcgatgc tggaattcca tgcggttcgg tacgcgggtg agcgtgtcgt
      121 agcgcgccag gtagcggatg cgcttctgcg tgcgatgcgc ctcgctgagg ttgcggatca
      181 ccagtaccca gcgacgcagg ccgtcgggct cgaccagcag gcagctcgtg aaggctacgg
      241 cagtggcctc gtcggtcggg gtcttgagtc gtgtgctgcc ctccggaagc agggcgtcgc
      301 gattgtcgaa cacgctgggc tcggcaaggt cgtgcaggtc tttgcccgcg agcgtatggg
      361 catctcgctg cagcagccgg gccgccgctt cgttggcctc gatgacacgg ccgtgctcgt
      421 cgcagacgat cacgccatcc tggacgcggt cgagcacctg ctccaggaac tggtacgtcc
      481 agcgggtcgc atcgaccgcc ggctcgccgg gctccgccgg ccggcctccg gccgggacca
      541 tgcgctgacc ggcgcgcagc cggcgcgtcc cgaaccaggc aatcagcagc ccgagcaaca
      601 gggccagcag cccgaccagc gcgacggcct gcgaccattc cgggcgcatc aggagcgcgg
      661 ctccggccgg gtagcgctgc ggggtgcgaa tgacaggcgc atgcggtcgg cgctcagccc
      721 ttgcggcgct cgtcgaaact gacgacgtcc gtgtccccca tggattgcag gtccgcctcg
      781 accacggcgc cctgcgagat cgagatgccg ttgccgacga cccggccgcg gacccgcgcc
      841 gaggcagtga tctcgacccg gccgcgcgcc agcagctcgc cgtcgagctc gccggccagg
      901 atcacgtcat cggcccggat ggcgccctgc cagcgcccgc cctcggccag cgtcaggccg
      961 ccctcgatgt cggcgtcccc ggtcacttgg ccggcgatca ggaaatggcc ttcgccctgc
     1021 agcgacccgt tgagccgcgc cccggggccg aggatagtgg ccggcgggcc gatgcgatcc
     1081 cgcattctcc ggttcttcat ccactgcctc cgctggccat cctgcatcct gtacacgcct
     1141 tgttcacgaa tgcaattgta tcgtttcctg tgacgaacgc ctcctgggag cctgtatgcc
     1201 tcgattccgg gccggaaaat tcctgctggc gctgctggtc ctcgccgccc tgggcgcggg
     1261 cagcggctgc gcaacctcgt tcctgtacga gcgggccgac cgcttcgcca atcgctggat
     1321 gggtgactac ctggagctcg atccggccca gcaggagtcg ctcgatacgg cgctcgccga
     1381 gctgcaccgc tggcaccgcc gcgaacagct gccggcctat gccgcctggc tgcggtccgc
     1441 cgccgcggac ctggctgccg gcggtcccta tgcggaacgg gcgctccggg ggcgcggcac
     1501 cgaactgggc ggcttctggc gcgagcttgc cgaagcgggg gcgcctgcgc tgaccgccgt
     1561 cggggccacc ttgtccgacg cccaggtgga ggtgctgctg gaggccctgc gggaggcgcg
     1621 gagcaaggag ttcgagtacg ccgctgcgcg cccggattcc taccatcagc agcgcagcgc
     1681 ccgctcgatg gagcgcttcc tcaggcgctg gaccgggcgc atgacggccg cgcagcaggc
     1741 catgatccgc gactgggcgg cagccctcga gccctcccgg gtagcgtcgc tcgagaaccg
     1801 cgccggctgg atcgaagcgc tggagcgggc gctcgagcgg cgcgcggatc cggcggcatt
     1861 acgccaggca gcagaaagcc tgttcgtggc gccgtcgtcg cgctgggacc ctgactacgc
     1921 ggccctggtg gaacgcaata ccgtgcacac caccaccttc atggccgagt tcctcgccgg
     1981 gctggatgaa cgccagcgcc ggcgggcggt cgaacggctg gagcggctgg ccgccgaatt
     2041 cgagcagctg gcagcggccg gcagctgaac cttgctcatg ttgcagtgcg catagctgcg
     2101 ccgcgtgggc gctgatttaa tgcccgctcc ttcaaccgac cggagagcct gagcgtggac
     2161 aagccctgga tccagagtta ccagcccgga acacctgccg agatcgatct cagcgacggg
     2221 gcgacgctgg tcagcatttt cgaggacagc gcgaaacgct tcgc
//
LOCUS       DAOMPG010000319         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_19344_length_2214_cov_2.662807, whole genome shotgun sequence.
ACCESSION   DAOMPG010000319 DAOMPG010000000
VERSION     DAOMPG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19344_length_2214_cov_2.662807"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..983
                     /gene="ilvC"
                     /locus_tag="PLS34_12520"
     CDS             15..983
                     /gene="ilvC"
                     /locus_tag="PLS34_12520"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRP88323.1"
                     /translation="MAPEPLAGRRVAVLGFGAQGRAQALNLRDSGVDVVVGLRPDSPR
                     LAECEEAGLAVRTPAAAAAECDVFAMLVPDEAQPQLYAEVLAPVLAPGAALLFAHGYN
                     IHYHRLAPRADLDVVMVAPLGIGEQVRRQYELGRGVPALLAVHQDATGRAAALAGAYA
                     AANGHARAAVFATSFAEETETDLFAEQAVLCGGLGQLITAAFETLTEAGYPEELAYFC
                     CLHEVKLIADLVQSRGIAGMRRSISSTAAYGDATRGARVIGPQSRQEMRALLAEIRDG
                     RFAAALEAEMAAGAPTLAAWRAAAQAHPIEAVGERLRALMPWLRQG"
     gene            1027..1842
                     /locus_tag="PLS34_12525"
     CDS             1027..1842
                     /locus_tag="PLS34_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HRP88324.1"
                     /translation="MNESQTSESAAMEEPRARRKGVYLLPNLITTGALFAGFSAVVAA
                     MDGNFATAAVAIYIAMVLDGLDGRVARMTSTESEFGKQYDSLADMVSFGLAPSLVFYL
                     WGAKALQELGWLWGKVGWLVAFFFAVAAALRLARYNSRLPSTDKRYFEGLPSPSAAAV
                     VAGLVWLSTVIEASGVLAMIVGLVVTAMAGGLMVSRLAYYSGKDLNLGGRIPFAYVLL
                     IPAVFILISLNPPTVLFLLFFTYSLSAPAVAAWRFMRRRRRRQLREDEPAGGD"
     assembly_gap    1879..1888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1889..>2214
                     /locus_tag="PLS34_12530"
     CDS             <1889..>2214
                     /locus_tag="PLS34_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRP88325.1"
                     /translation="WTRRADRVLPGAPEPVLPAPTAAPGDLRAPAGDGPAADVAALDW
                     AALEARVSGCMRCPLHAGRTRTVFGVGDREADWLIVGEAPGAEEDRRGEPFVGQMRIA
                     GQDRAA"
BASE COUNT          278 a          744 c          851 g          331 t
ORIGIN      
        1 tgacgaggga gcgcctggcc ccggaaccgc tggcgggccg gcgggttgcg gtgctcggct
       61 tcggcgccca gggccgggcg caggcgctca acttgcgcga ctccggcgtg gacgtggtgg
      121 tcgggctgcg cccggattca ccgcggcttg cggagtgcga ggaggcggga ctggcagtgc
      181 gcacgcccgc cgcggccgcg gccgagtgcg acgtgttcgc catgctggtg ccggacgaag
      241 cgcagccgca gttgtacgca gaggtcctgg cgccggtgct cgcgcccggc gcggcgctgt
      301 tgtttgcgca cggctacaac attcactacc accggctggc gccgcgcgcc gacctcgacg
      361 tggtgatggt ggcgccgctg gggatcggcg agcaggtgcg gcgccagtac gagctgggcc
      421 gcggtgtgcc ggccctgctc gccgtgcacc aggacgcgac cggtagggcg gccgcgctgg
      481 cgggcgccta cgccgccgcc aacggccacg cgcgcgccgc ggtgttcgca accagcttcg
      541 ccgaggagac cgagaccgac ctgttcgcgg agcaggcggt gctgtgcggc ggcctcgggc
      601 agctgatcac cgcggccttc gagaccctga ccgaggcggg ctacccggag gagctggcgt
      661 atttctgctg tctgcacgag gtcaagctga tcgccgacct ggtgcagtcg cgcggcatcg
      721 cgggcatgcg ccgctcgatc tccagcaccg cggcctacgg cgatgccacc cgcggagcgc
      781 gggtcatcgg gccgcaaagc cggcaggaaa tgcgcgcgct gctggccgag atccgcgacg
      841 ggcgtttcgc ggcggcgctg gaggcggaaa tggcggccgg cgcgcccacc ctggcggcct
      901 ggcgcgccgc cgcccaggcg cacccgatcg aggccgtggg agagcgcctc agggcgctca
      961 tgccgtggct caggcagggc tgagcgctgg aattcccccc ggcgacatgc gtaaatgatc
     1021 ggcgaaatga acgaatcgca gacgagcgaa tcggcagcca tggaagaacc ccgcgccagg
     1081 cgcaagggcg tctacctgtt gccgaacctg atcaccaccg gcgcgctgtt cgccggcttt
     1141 tccgcggtcg tggccgcgat ggacggcaat ttcgccaccg ctgcggtggc catctacatc
     1201 gccatggtgc tggacggcct ggacggccgg gtggcgcgca tgaccagcac cgagagcgag
     1261 ttcggcaagc agtacgacag cctggccgac atggtgtcgt tcggcctggc gccctcgctg
     1321 gtgttctacc tgtggggcgc gaaggccctg caggagctcg gctggctgtg gggcaaggtg
     1381 ggctggctgg tggcgttctt ctttgccgtc gcggcggccc tgcggctggc gcgctacaac
     1441 tcgcgactgc cttccaccga caagcgctac tttgagggcc tgcccagccc gtcagcggcg
     1501 gcggtggtgg cgggcctggt gtggctaagc acggtcatcg aggcatccgg cgtgctggcg
     1561 atgatcgtcg gtctggtggt gaccgcgatg gcgggtgggc tgatggtgag ccggctggcc
     1621 tattacagcg gcaaggacct caacctgggc gggcgcattc cattcgccta tgtgctgctg
     1681 atcccggcgg tgttcatcct catcagcctc aatccgccga ccgtgctgtt cctgttgttc
     1741 ttcacctatt cgctgtccgc gccggcggtg gcggcctggc gcttcatgcg ccgccggcgg
     1801 cgccggcagc tgcgggagga tgaacccgcg ggcggggatt gaagtggacg cgcgccggct
     1861 ggcgagcctc gacgccatnn nnnnnnnnct ggacccgccg cgccgaccgg gtgctgcccg
     1921 gcgcgccgga gccggtgctg ccggccccaa cggccgcgcc tggcgatctt cgcgcgccgg
     1981 ccggcgacgg tccggcggcc gacgtggctg cgctcgactg ggccgcgctg gaggcgaggg
     2041 tcagcggctg catgcgctgc ccgctgcatg ccgggcggac gcgcacggtg ttcggcgtgg
     2101 gcgaccggga agccgactgg ctgatcgtcg gcgaggcgcc cggcgcggag gaggaccgcc
     2161 gtggcgagcc gttcgtcggc cagatgcgga tcgccggtca ggaccgcgcc gcca
//
LOCUS       DAOMPG010000320         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_19614_length_2190_cov_4.818267, whole genome shotgun sequence.
ACCESSION   DAOMPG010000320 DAOMPG010000000
VERSION     DAOMPG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19614_length_2190_cov_4.818267"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLS34_12535"
     CDS             complement(<1..371)
                     /locus_tag="PLS34_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88326.1"
                     /translation="MNLLVFTTRGGRTRQIPLSRRLVVAGALCAFVLAFGAGLLAGRM
                     SLPSSEPELFAELRDALEAQRAEVEAVREQSDRNVDALAIRLGQLSAHIVRLDALGQR
                     LVNMAGLEDGEFNFGSTPPQGG"
     regulatory      complement(447..548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            631..900
                     /locus_tag="PLS34_12540"
     CDS             631..900
                     /locus_tag="PLS34_12540"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRP88327.1"
                     /translation="MLAGRDLAQLVERAREAGELDARVRALLPEGLGAHVTGAVQHED
                     TVVILADSAAWASRIRFHAPELLARLGPRFDGAVTRVRVKVRPGN"
     gene            1058..1252
                     /locus_tag="PLS34_12545"
     CDS             1058..1252
                     /locus_tag="PLS34_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88328.1"
                     /translation="MRSSDRYIKIVKWSDEDQCYVGTAPGLVFGGCHGSDESAVREEL
                     GQIVEEAIALYEQDGRPLPA"
     gene            complement(1393..>2190)
                     /gene="lpxC"
                     /locus_tag="PLS34_12550"
     CDS             complement(1393..>2190)
                     /gene="lpxC"
                     /locus_tag="PLS34_12550"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRP88329.1"
                     /translation="IVFRRTDLDPTVEIRADGMHVGETTLGTTLVRDGVRVATVEHLM
                     SALAGLGIDNAIVELSAQEVPIMDGSAGPFVFLIQSAGIAEQGAAKRFIRVRRPVEVR
                     DGDKWARFAPYDGFKIDFEIEFDHPVFKRHSQRAAMDFATTSFLREVSRARTFGFMRD
                     MEFLRAMNLGRGGSLDNAIVLDDYRILNDDGLRYENEFVRHKILDAIGDLYLIGHSII
                     GEFSGFKSGHGLNNQLLRALVRNESAWEEVTYEDERDAPFNFAVAAA"
BASE COUNT          350 a          736 c          763 g          341 t
ORIGIN      
        1 ccgccctgcg gcggcgtgga cccgaaattg aactcgccgt cctcgaggcc ggccatgttg
       61 accagccgct gaccgagggc gtcgaggcgc acgatgtggg ccgacagctg gccgagccgg
      121 atggccagcg cgtcgacgtt gcggtcggac tgctcgcgca cggcctccac ctctgcccgc
      181 tgggcctcca aggcgtcgcg cagttccgcg aacaactccg gctcggagga cggcagggac
      241 atgcggccgg cgagcaggcc ggcgccgaag gccagcacga acgcacacag ggcacccgca
      301 acgacgagcc gacggctgag cggaatctgc cgcgtcctgc cgccacgcgt cgtgaagacg
      361 aggaggttca acggacctcc gcttcgctgg ggcatcgcgc cggaaggttc ccggcacggc
      421 ggtttctgga tactgattcc atactgctcg gcagctccgc tgtcgtaagt ccggccccgc
      481 gggggcacgg gaatctcaga ccggtggctt tgcgacggcg cctcgcgacg ccctggcctt
      541 ttacaagctt gtcgactatg cagcaaaatc cctcctcgct caagtgaggg aattcacata
      601 aacggcaggg aacccaagct gctcaaggac ttgctggccg gccgggacct ggcccagctg
      661 gtcgagcgcg cccgcgaggc cggcgaactc gacgccaggg tgcgcgcgct cctgcccgag
      721 ggcctcggcg cgcacgtcac cggggccgtc cagcacgagg acaccgtggt gatcctggcg
      781 gacagcgcgg cctgggccag ccggatccgc ttccatgccc cggaactgct ggcgcgcctc
      841 gggccgcgct ttgacggggc cgtgacccgg gtgcgggtca aggtgcggcc ggggaactag
      901 gcctctggct tttgttggtc caccgcggcg gagaggcgcc atgggcccgc ctcgcttaga
      961 aatctttatg cgctccgcgg gcccatgaca cccctccgcc gctgcggcgc ggatggccgg
     1021 aggcctggtc ccggggcgcc cttgcggagc tggtgaaatg agatcgagcg accgatacat
     1081 caagatcgtg aagtggtccg acgaggacca gtgctacgtc gggaccgcgc ccggcctggt
     1141 gttcggcggg tgccacggta gtgacgagag cgccgtccgc gaggaactcg gccagatcgt
     1201 cgaagaggcg atcgctcttt acgagcagga cggccggccc ctccccgcct gacctacccc
     1261 agcccgacct acccgagcct gcccctatgc aacgccgtag cgccagcggg gcgtgatggg
     1321 cccgctgagc gcataaagat ttctaagtga agcgggcccg tcgcgcctcg ctggcgcggt
     1381 cgaactgcac agtcaggccg cagccaccgc gaagttgaac ggcgcgtcgc gctcgtcctc
     1441 gtaggtgacc tcttcccagg ccgactcgtt gcgcaccagc gcgcgcagca gctggttgtt
     1501 cagcccgtgg ccggacttga agccgctgaa ttcgccgatg atgctgtggc cgatcaggta
     1561 caggtcgccg attgcgtcga gaatcttgtg gcggacgaat tcgttctcgt aacgcagccc
     1621 gtcgtcgttg aggatgcggt agtcgtcgag cacgatggcg ttgtccaggc tgccgccgcg
     1681 ccccaggttc atggcgcgca ggaattccat gtcgcgcatg aagccgaagg ttcgcgcccg
     1741 actgacttcc cgcaggaagg aggtggtggc gaaatccatg gccgcgcgct gcgagtggcg
     1801 cttgaacacg gggtggtcga actcgatctc gaaatcgatc ttgaagccgt cgtagggggc
     1861 gaagcgcgcc cacttgtcgc cgtcgcgcac ctcgaccggg cggcgcacac ggatgaagcg
     1921 cttggcggcg ccctgctcgg cgatgccggc cgactggatc aggaacacga aggggccggc
     1981 gctgccgtcc atgatcggca cttcctgcgc cgacagctcg acgatggcgt tgtcgatgcc
     2041 gagccccgcc agggccgaca tcaggtgctc gaccgtggcg acgcgcacgc cgtcgcggac
     2101 cagggtggtg ccgagcgtgg tctcgcccac gtgcatgccg tcggcgcgga tctctaccgt
     2161 cggatcgagg tcggtgcgcc ggaacacgat
//
LOCUS       DAOMPG010000321         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_19832_length_2171_cov_2.817580, whole genome shotgun sequence.
ACCESSION   DAOMPG010000321 DAOMPG010000000
VERSION     DAOMPG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19832_length_2171_cov_2.817580"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLS34_12555"
     CDS             complement(<1..1683)
                     /locus_tag="PLS34_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRP88330.1"
                     /translation="MIRIASLPAVLLALLLAPALAAAATDAAPKADQKDPAWSVNAPP
                     GDWQAIAIDTREFSWADVDVSPDGRHIVFHMLGDIYRVGIDGGEAEALTADIAWNFQP
                     RFSPDGRSIAFISDRDGAENLWIMDADGGNPVQVSKEREHLLHNPAWSPDGDYLAARK
                     GYVSRRSIPAGSIWLYHRGGGGGVELVERLHGAQSQKNIAEPNFSPDGRYLYFSQDMT
                     SGQQWEYNKDANTGIFAVRRLDRETGQVETIVSGPGGAIRPVVSPDGRQLAFVRRNAE
                     TLSSRLMVKDLVSGIERTLFDGLDRDKQETSGDMGNYPAYDWLPDGSGLVFWSGGGFH
                     RVGLDGRHRPIEVRVRAEKQVHPALQRKVQVDADEFDVRMLRWSQLSPDGRMAVYQAL
                     GYLWVHDLERGERRRLTRQTDHWEFHPAFSPDGRHVVYTTFDDEALGAVRIAPVGRGR
                     AQVLTTEPGHYVEPAFSADGRRVAFRKITGGYLTSPNWSQNPGLYVVDSDGRNLKRVL
                     DKGARPQFSPDGTRLLHSRNTEQGHLALYSTNLDGHDERQHLSGEWITEYRVS"
     gene            complement(1797..>2171)
                     /locus_tag="PLS34_12560"
     CDS             complement(1797..>2171)
                     /locus_tag="PLS34_12560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP88331.1"
                     /translation="LKEPSVCVTTWGDGFRLGSTMEFSGYDARLNPVRLAALRRAATE
                     YLHDPGAGEPQECWQGWRPMTWDDLPVIGRAPRAASVIIAAGHGMLGVSMAAVTGLLV
                     RDLALGRPPLLDLAPLSPARFA"
BASE COUNT          297 a          731 c          811 g          332 t
ORIGIN      
        1 ggagacgcgg tattcggtga tccactcgcc ggacaagtgc tggcgctcgt cgtggccgtc
       61 gagattggtg ctgtagagcg cgaggtggcc ctgctcggtg ttgcgcgagt gcagcagccg
      121 cgtgccgtcg ggcgaaaatt gcggccgggc gcccttgtcc agcacgcgct tgaggttgcg
      181 cccgtcgctg tcgacgacgt acaggccggg gttctgcgac cagttcggcg aagtcaggta
      241 gccgccggtg atcttgcgga aagcgactcg ccggccgtcc gcagagaagg ccggctcgac
      301 gtagtggccg ggctcggtgg tgagcacctg cgcgcggccg cggcccaccg gcgcgatgcg
      361 caccgcgccg agcgcctcgt cgtcgaaggt ggtgtagacc acgtggcggc cgtcgggcga
      421 gaaggccggg tggaactccc agtgatcggt ctggcgggtc aggcggcggc gctcgccgcg
      481 ttccaggtca tgcacccaga ggtagccgag cgcctggtag acggccatgc ggccatccgg
      541 gctgagctgc gaccagcgca gcatgcgcac gtcgaactcg tcggcgtcca cctgcacctt
      601 gcgctgcagt gccgggtgga cctgcttctc cgcgcgcacc cgcacttcga tcggccggtg
      661 gcggccgtcg agcccgacac ggtggaagcc gccgcccgac cagaacacca gcccgctgcc
      721 gtcgggcagc cagtcgtagg ccgggtagtt gcccatgtcg ccgctggttt cctgcttgtc
      781 gcggtccagc ccgtcgaaca gcgtgcgctc gatcccggac acgaggtcct tgaccatcag
      841 gcggctgctg agcgtctcgg cattgcggcg cacgaaggcg agctggcggc cgtccggcga
      901 gaccacgggg cggatggcgc cgcccggccc cgacacgatg gtctcgacct ggcccgtttc
      961 gcgatccagc cggcggacgg cgaagatgcc ggtgttggcg tccttgttgt actcccactg
     1021 ctggccggag gtcatgtcct ggctgaagta caggtaacgg ccgtccggcg agaagttcgg
     1081 ctcggcgatg ttcttctgcg actgcgcgcc atgcagccgc tcgacgagtt ccacgccgcc
     1141 gccgccgccg cggtggtaga gccagatcga gccggccggg atgctgcggc gggacacgta
     1201 gcccttgcgc gcagcaaggt agtcgccgtc gggcgaccac gccgggttgt gcagcaggtg
     1261 ttcgcgctcc ttgctgacct gcaccgggtt gccgccgtcg gcgtccatga tccagaggtt
     1321 ctccgcgccg tcgcggtcgc tgatgaaggc gatgctgcgg ccgtccgggc tgaagcgcgg
     1381 ctggaagttc cacgcgatgt ccgcggtcag cgcctcggcc tcgccgccgt cgatgccgac
     1441 gcgatagatg tcgcccagca tgtggaacac gatgtgccgg ccgtcggggc tgacgtccac
     1501 gtccgcccag ctgaactcgc gggtgtcgat ggcaatggcc tgccagtcgc cgggcggcgc
     1561 gttgaccgac caggccgggt ccttctggtc ggctttcggg gccgcgtcgg tggcggccgc
     1621 ggcgagcgcg ggggcgagca gtagcgcgag cagtacggcg ggcagcgaag caatgcgaat
     1681 catgcagtgg tccttcaagg cgggccgtcg gtcagccgac ggcgtcaatg gcagcggcga
     1741 atgatagcgg cgtcgagtcg tgcaggcacc ttgcaccacg gcagggcggg gctgcgctag
     1801 gcgaagcgcg ccggcgaaag cggcgccagg tccagcagcg gcggcctgcc cagcgccagg
     1861 tcacgcacca gcaggcccgt gacggccgcc atgctgacgc cgagcatgcc gtggccggcg
     1921 gcgatgatga cgctcgccgc gcgcggcgcg cggccgatga ccggcaggtc gtcccaggtc
     1981 atcggccgcc agccctgcca gcactcctgc ggctcgcccg cgccgggatc gtgcaggtac
     2041 tcggttgcgg cccggcgcag ggccgcgagg cgcaccgggt tgaggcgcgc gtcgtagcct
     2101 gagaattcca tggtgctgcc cagccggaag ccgtcgcccc aggtggtcac gcatacgctg
     2161 ggctccttga g
//
LOCUS       DAOMPG010000322         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20007_length_2155_cov_5.122857, whole genome shotgun sequence.
ACCESSION   DAOMPG010000322 DAOMPG010000000
VERSION     DAOMPG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20007_length_2155_cov_5.122857"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLS34_12565"
     CDS             <1..1107
                     /locus_tag="PLS34_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRP88332.1"
                     /translation="NGAGERFLVELEPEKQELASRDVVSRHMMSEILYRRGVDSPNGP
                     HLWLDLRHLGEEHLTTRLREIYEICRDFLGIRPWEQLVPVRPTQHYSMGGIRVNKDGQ
                     AYGLGGLFAVGEAACWDMHGFNRLGGNSLAETIVAGRIVGARVAEFVARPGPGPDAVL
                     AKSALQAQEARIAELVGRPAGGRSLYQLRDAMGEIMMNHLGVFRSGDEIEFAISELQA
                     LIREVAGVGLRSTAPGPNPELAYALRLPGMLRLALVTAMGALARKESRGAHYRTDYPQ
                     RNDAEWLNRTLARWRAGAEAPELNYEPVGLLDLPPGHRGYGASSQVEMTTSIEQYNAQ
                     VAAAQAAAGRLATDEPLGARLRPGAWRGAFSAPA"
     gene            1122..1877
                     /locus_tag="PLS34_12570"
     CDS             1122..1877
                     /locus_tag="PLS34_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HRP88333.1"
                     /translation="MSSQAARTLKFEIFRYNPEDPGSKPHMQSFELQEVPFMSLFMAL
                     NKLREEQDPTLQFDFVCRSAVCGSCGMMVNGRPSLACKTLTSQLDSTVRLHPLPAFRL
                     VGDLSVDTGSWFRGMSERIQGWIEEKTSFDPEAPEERMDDDLAQAIYERERCIECGCC
                     VASCAVANTRPAFVAPAGLNRIARFMMDPRDRRKQGDWFEVVASDEGVFGCLGLMACQ
                     DVCPKELPLREVYAYLRRKSLTGLLGKKTAKPG"
BASE COUNT          388 a          690 c          729 g          348 t
ORIGIN      
        1 aacggcgccg gcgagcgctt cctggtcgag ctggagcccg aaaagcagga gctggccagc
       61 cgggacgtcg tgtcacgcca catgatgagc gagatcctgt atcggcgcgg cgtggacagc
      121 ccgaacggcc cgcacctgtg gctggacctg cgccacctcg gcgaggagca cctgaccacc
      181 cggttgcgcg agatctacga gatctgcagg gatttcctcg gcattcgtcc ctgggaacag
      241 ctggtgcccg tgcgcccgac ccagcattac tcgatgggcg gcatccgggt gaacaaggac
      301 ggccaggcct acggcctcgg cggactgttc gcagtggggg aggcggcctg ctgggacatg
      361 cacggcttca atcgcctggg cggcaacagc ctggccgaga ccatcgtcgc cggccgcatc
      421 gtcggcgccc gcgtggcgga attcgtggcg cggccgggcc cgggcccgga tgcggtgctc
      481 gcgaagtcgg cgttgcaggc ccaggaggcg cgcatcgccg agctggtcgg acgcccggca
      541 ggcggacgtt cgctctacca gctgcgcgac gccatgggcg agatcatgat gaatcacctc
      601 ggggtgttcc gcagcggcga cgaaatcgag ttcgcgatca gcgaactgca ggccctgatt
      661 cgcgaggtgg cgggcgtcgg cctgcgctcg accgcgccgg ggccgaatcc cgaactcgcc
      721 tatgccttgc gcctgccggg catgctgcgg ctcgcactcg tcaccgccat gggggcgctg
      781 gcgcgcaagg aaagccgtgg cgcccattac cgcaccgatt atccgcagcg caacgatgcc
      841 gaatggctga accggaccct ggcgcgctgg cgggcggggg cggaggcccc ggagttgaac
      901 tacgaacccg tgggcctgct ggacctgcca ccgggccacc gcggctacgg cgcgagcagc
      961 caggtggaga tgaccacgag catcgagcaa tacaacgcac aggtcgctgc tgcccaggcc
     1021 gccgcgggcc gactcgccac ggacgagccc cttggcgccc ggttgcgtcc cggcgcctgg
     1081 cgcggcgcct tcagcgcgcc cgcctgacga ggaagcgaag catgtccagc caggccgcac
     1141 gtaccctgaa gttcgaaatc ttccgctaca acccggagga tcccggctcg aagccgcaca
     1201 tgcagagctt cgaactgcag gaagtcccgt tcatgtcact cttcatggca cttaacaagt
     1261 tacgtgaaga acaggatcca acactccaat ttgacttcgt ttgtcgttca gccgtctgcg
     1321 gttcctgcgg gatgatggtg aacggcaggc cgtcgctggc ctgcaagacc ctgacttcgc
     1381 agctggacag caccgtgcgc ctgcatccct tgccggcgtt ccggctggtg ggcgacctgt
     1441 cggtggacac cggcagctgg tttcgcggca tgtctgagcg aatccaggga tggatcgagg
     1501 aaaagacgtc gttcgatccg gaagctcccg aagagcgcat ggacgacgat cttgcccagg
     1561 cgatctacga gcgggagcgc tgcatcgagt gcggctgctg cgtggcgtcc tgcgcggtgg
     1621 ccaacacgcg cccggccttc gtggcgccgg cggggctgaa ccggatcgcg cgcttcatga
     1681 tggatccacg cgaccggcgc aagcagggtg actggttcga ggtcgtggcc agtgacgagg
     1741 gcgtgttcgg ctgcctcggc ctgatggcct gccaggacgt gtgcccgaaa gagctgcccc
     1801 tgcgcgaggt gtacgcctac ctgcggcgca agtcgctcac cggcttgctt ggcaagaaga
     1861 ccgccaagcc cggctgaggc tgtagtgttt cgctgacctc gatgagcagc ccgcaaaaca
     1921 ggacaggtct tcggaatcaa ctcgaaagat ggcctgcaag catttgaaga taaagactac
     1981 gaagtggggg ttgcaggctt ccagcccgat ttcaggtcga catatcagtt aacataatat
     2041 acattatgcg cagtaagatc tccggcctat cggggcatgg tgacctcaaa gcgaatcgca
     2101 tcgagctgcc gcggcgactt gcccatcgac tccagcaagg gcaaccagcg cggat
//
LOCUS       DAOMPG010000323         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20057_length_2150_cov_4.990931, whole genome shotgun sequence.
ACCESSION   DAOMPG010000323 DAOMPG010000000
VERSION     DAOMPG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20057_length_2150_cov_4.990931"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLS34_12575"
     CDS             <1..396
                     /locus_tag="PLS34_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP88334.1"
                     /translation="AATLVDQGGRYVLVVGDDPYQHRLFESMLGDEPIELQFATSAQH
                     GLELMHHRSPAAVLMDINMPEVDGVEATRRIKSVHRFQPIPVIMVTGLGTRDRVVESR
                     QAGASEFLVKPVNRDALLAKLRKVGVLPS"
     gene            487..702
                     /locus_tag="PLS34_12580"
     CDS             487..702
                     /locus_tag="PLS34_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88335.1"
                     /translation="MTRWKEIAEAIRELQEEGQMDVFVGEHSVTFEVAVDDQGIYLTI
                     CPHCIGEAVNLALGGEEQSAPPDCEVH"
     gene            complement(736..1173)
                     /locus_tag="PLS34_12585"
     CDS             complement(736..1173)
                     /locus_tag="PLS34_12585"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HRP88336.1"
                     /translation="MSDVWVVVASSTRCRIFAQHKHSGPLEPVAELEHPEGRLRARDL
                     ASDRPGRAFDSEGNGRHAMGQPVDPTVQENIRFAKTVADKIDAARKKDRFGRLVLVAD
                     PRFLGHLRNGLSSATRQRLTTELQKNLADADPKAIRSALPYRI"
     gene            1445..>2150
                     /locus_tag="PLS34_12590"
     CDS             1445..>2150
                     /locus_tag="PLS34_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88337.1"
                     /translation="MSEDNSPEAVSRISADLGAAVRRSRATPSRVGLNPAITKVTVDL
                     LNLDPETAEQTLSDSLEVIREAIGADSVCFTLLTDDGEAFDTVSHASNVLAGCRPERL
                     AGARMADVEWLSGKLGHMRLVEIRDARRLSPGLIGETRLMNEMGAGGALFAGLDIHGK
                     LGGVLGVLSARPVDTWHVDVYLLLKLISASLSAALTRLKLNQELHRIYERDELTRHAS
                     NDGVWDFDVETNTIRFS"
BASE COUNT          358 a          699 c          727 g          366 t
ORIGIN      
        1 gcggcgacgc tcgtcgacca gggcggccgg tacgtgctgg tcgtcggcga cgacccgtac
       61 cagcatcggc tcttcgagag catgctgggc gacgagccca ttgagctcca gttcgccacc
      121 tcggcgcagc acggcctcga gctcatgcat catcgttcgc ctgcggccgt cctgatggac
      181 atcaacatgc cggaagttga cggcgtcgag gcgacacgac ggatcaagtc cgtgcaccgc
      241 ttccagccga tcccggtgat catggtgacc ggcctcggca cgcgggaccg ggtggtggag
      301 agccgccagg ccggcgccag cgagttcctg gtcaagccgg tgaaccgtga cgccctgctg
      361 gcgaagctgc gcaaggtcgg cgtgctgccg agctgaacag gtcgcagtga gtaccgaggg
      421 tgcttgcggg ccgtgtccgg cttacaatcc gctcctgccc gcatcggaac ccggatggag
      481 agtgacatga cgcgctggaa agaaatcgcc gaagccatcc gggagctcca ggaagagggg
      541 cagatggacg tgttcgtggg cgagcactcg gtcactttcg aagtggcggt ggacgaccag
      601 ggcatctacc tgacgatttg cccgcattgc atcggcgagg ccgtgaacct ggcgctgggc
      661 ggcgaagagc agagcgcccc gccggattgc gaggtgcact aggccgacat tcgaaccccg
      721 ccgaccgcgc cacgctcaga tgcgataggg cagtgcgctg cggatggcct tggggtcggc
      781 gtcggccagg ttcttctgca gctcggtggt caggcgctgg cgggtcgccg acgacaggcc
      841 gttgcgcagg tggccgagaa agcgcgggtc cgccaccagc accagccggc cgaagcgatc
      901 tttcttgcgc gccgcgtcga tcttgtcggc gaccgtcttg gcgaagcgga tgttctcctg
      961 cacggtgggg tccaccggct ggcccatggc gtgccggcca ttgccttcgc tgtcgaaggc
     1021 ccgccccggg cggtcgctcg ccaggtcccg ggcgcgcagc cgtccctcgg gatgttccag
     1081 ttctgctacg ggttccagtg ggccactatg cttgtgctga gcgaaaattc tgcagcgggt
     1141 gcttgacgct acgacgaccc agacatcact catagtattc ggcctccgtg gaagtgttgc
     1201 atgcccgccg tgacgggcgg ccacgacgct caccctgagc gtagtcggcg ctcgttggca
     1261 tttccagcaa ggggctgcat gacgctcgcc cctgcgcctg ctggagcaat tgtgacgctg
     1321 ttcctgcagc attgacgccc ggtcttgtaa tctccacgct tcatgtccag aatgccgaac
     1381 gaaaagaaaa cggcaggaac aaggacttac ggatactatt taaggccctg catttggatc
     1441 ctgcatgagc gaagacaact cgcccgaagc ggtgtcccgg atcagcgctg atctgggtgc
     1501 tgccgtacgc aggtcccggg cgaccccgtc gcgagtcggc ctgaatccgg ccatcaccaa
     1561 ggtgacggtc gatctgctca acctcgatcc ggaaaccgcc gagcagaccc tgagcgattc
     1621 gctcgaggtc atccgcgagg ccatcggcgc ggactcggtc tgctttacgc tgctcaccga
     1681 cgacggcgag gctttcgata ccgtcagcca cgcctcgaac gtcctcgccg gttgccgccc
     1741 ggagcgcctg gcgggcgcgc gcatggccga cgtcgagtgg ctgagcggca agctcggcca
     1801 catgcggctg gtggagattc gcgacgcgcg gcgcctgtcg ccgggcctga tcggcgaaac
     1861 ccggctgatg aacgaaatgg gcgccggcgg cgcgctgttc gccggcctgg acatccacgg
     1921 caagctcggc ggcgtgctgg gcgtgttgtc ggcgcggccg gtggacacct ggcacgtcga
     1981 cgtctacctg ctgctcaagc tcatcagtgc cagcctgtcg gcggcgctca cgcggctgaa
     2041 gctcaaccag gagctgcacc gcatctacga gcgcgacgag ctgacccggc acgcctcgaa
     2101 cgacggcgtg tgggacttcg acgtggagac caacaccatc cgcttctcgc
//
LOCUS       DAOMPG010000324         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20199_length_2138_cov_4.606337, whole genome shotgun sequence.
ACCESSION   DAOMPG010000324 DAOMPG010000000
VERSION     DAOMPG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20199_length_2138_cov_4.606337"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..738
                     /locus_tag="PLS34_12595"
     CDS             187..738
                     /locus_tag="PLS34_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRP88338.1"
                     /translation="MFWIVLVLLAALLLWGVLIYNRLVRERNQVRSAWSDIDVQLTRR
                     HDLVPQLVEAVKAYAGYERATLTAVTELRARAAAAGSLRDKARLEDEMEAGLHRLIAL
                     AESYPELKADGNFLQLQRDLVEVEDHLQYARRFYNGAVRIYNTRIESVPDMLVAQPFR
                     FAPAEFFAVDDAEVRAAPRVALG"
     gene            740..>2138
                     /locus_tag="PLS34_12600"
     CDS             740..>2138
                     /locus_tag="PLS34_12600"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRP88339.1"
                     /translation="MRRATATLLLLTLAAIVPAAAAERILDYRSDIEVKADATVIVAE
                     SIAVEARGEIIRRGIYRDFPTDYRDRLGNRYRVAFEVLEVTRNGAPEPYHTERRANGV
                     RVYVGSADRFVEHGRQDYRIVYRTDRQLGYFENHDELYWNVTGNGWDLPIERAAAVVR
                     LPDGVPMAEVTMEAYTGRQGSTEQSYAADLDGRAAVIAATRALGAREGFTIVVGWPKG
                     HVHEPGPAERLERTLVDNRGLLLALIGLLLVIGYLGWAWARYGRDPQKGVIFPHYAPP
                     DKYSPASARYIMRMGYDNRAFTAAVLSLAVKGHVEIDEQGGKYTLVKLDSDQPLAPGE
                     QALKDALFAKAVIVELDNKNHALMSAAKRAHEQSLKRDYRKIYFFTNSNLLLPSLGAT
                     AALALAIGLLDAFRPMVFVVLGVIVLAHFVFAWLLRAPTARGRLLMDKLEGFRSYLEI
                     AEKDELNLRNPPEKTP"
BASE COUNT          335 a          724 c          750 g          329 t
ORIGIN      
        1 cggcgctgca gtggctggtg tacgtcggcg cgggcctcgg gctcgggctg ctgctttggc
       61 tggccgccgc caggctctat catcgcgaga agctggcgat cagcgcctga attgggcgcc
      121 tgaattgggg acattcccca ataacgcgtt attggggaat gtccccaatt ccgaggagga
      181 ccgccgatgt tctggattgt gctggtgctg ctggcggcgc tgctgctgtg gggcgtgctg
      241 atctacaacc ggctggtgcg tgagcgcaac caggtgcggt ccgcctggag cgacatcgac
      301 gtgcagctga cgcggcgcca cgacctggtg ccgcagctgg tggaggcggt caaggcctac
      361 gccggctacg agcgcgccac gctgaccgcc gtgaccgagc tgcgcgcgcg cgccgcggcg
      421 gccggcagcc tgcgcgacaa ggcgcggctg gaggatgaga tggaggcggg gctgcaccgg
      481 ctcatcgcgc tggccgagtc ctacccggag ctcaaggccg acggcaactt cctgcagctg
      541 cagcgcgacc tagtcgaggt cgaggatcac ctgcagtacg cgcggcgctt ctacaacggc
      601 gcggtgcgca tctacaacac gcgcatcgag agcgtgcccg atatgctggt ggcgcaaccc
      661 ttccgtttcg cgccggccga gttcttcgcc gtggacgacg cggaagtgcg ggcggcgccg
      721 cgcgtggcgc tcggctgaca tgcggcgcgc gacggcgaca ttgctgctgc tcaccctggc
      781 cgccatcgtg ccggccgcgg ccgccgagcg catcctcgat taccgcagcg acatcgaggt
      841 caaggccgac gccacggtga tcgtggccga aagcatcgcc gtcgaggcgc gcggcgagat
      901 catccggcgg ggcatctacc gcgatttccc gaccgactac cgcgaccggc tcggcaaccg
      961 ctaccgggtc gccttcgagg tgctggaagt gacgcgcaac ggcgcgcccg agccgtacca
     1021 cacggagcgc cgcgccaacg gcgtacgcgt gtacgtcggc agcgccgacc gcttcgtcga
     1081 gcatggccgg caggactacc gcatcgtcta ccggaccgac cggcagctcg gctatttcga
     1141 aaaccacgac gagctgtact ggaacgtcac gggcaacggc tgggacctgc ccatcgagcg
     1201 cgccgccgcg gtcgtgcggc tgccggacgg cgtgcccatg gctgaggtca ccatggaggc
     1261 atataccggc cggcagggca gcaccgagca gagctatgcc gccgaccttg acggccgcgc
     1321 cgccgtgatc gccgccaccc gggcgctcgg cgcgcgcgag ggcttcacca tcgtggtggg
     1381 ctggccgaag ggacacgtcc acgagccggg cccggcggaa cgcctcgagc gcacgctggt
     1441 ggacaaccgc ggcctgctgc tggccctcat cgggctgctg ctggtgatcg gctacctcgg
     1501 ctgggcgtgg gcgcgctacg gccgcgaccc gcagaagggc gtgatcttcc cgcattacgc
     1561 gccgccggat aaatattcgc cggcgtccgc ccgttacatc atgcgcatgg gctacgacaa
     1621 ccgcgccttc acggccgcgg tcctgagcct ggccgtcaag ggccatgtcg agatcgacga
     1681 acagggtggc aagtacacgc tggtgaagct cgactccgac cagccgctgg cccccggcga
     1741 gcaggcgctc aaggatgcgc tgttcgccaa ggcggtgatc gtcgagctcg acaacaagaa
     1801 ccatgcgctc atgtcggcgg cgaagcgggc ccatgagcag tccctgaaac gcgattaccg
     1861 caagatctac ttcttcacca actccaacct gctgctgccg tcgctcggcg cgaccgccgc
     1921 gctggcgctg gcgatcggcc tgctggacgc gttccggccg atggtgttcg tggtgctggg
     1981 cgtcatcgtg ctggcgcatt tcgtgtttgc ctggctgctg cgggcgccga ccgcacgcgg
     2041 ccggctgctg atggacaagc tggagggctt ccgcagctat ctcgagatcg ccgagaagga
     2101 cgagctgaac ctgcgcaatc cgcccgagaa gacgccgg
//
LOCUS       DAOMPG010000325         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20360_length_2123_cov_3.128627, whole genome shotgun sequence.
ACCESSION   DAOMPG010000325 DAOMPG010000000
VERSION     DAOMPG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20360_length_2123_cov_3.128627"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLS34_12605"
     CDS             <1..788
                     /locus_tag="PLS34_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP88340.1"
                     /translation="GDFSARAHAGSSDEFGRLAADFNLLARTLERNEAMRRRMMADVA
                     HELRTPLAVVQGELAAIEDGIRPFAAETLASLQAETRALGKLIDDLYQLSLSDVGALD
                     YRRRDLDLRVLVGEALQPLRERCAAAGLSLDDEAVAGAPLSVHADPDRLVQLVANLVE
                     NAIRYTDAPGRIVVACRQAEGHAVLEVSDTAPGVPPELQPQLFERFFRVDTSRSRATG
                     GAGLGLAICRNIVEAHNGTITTAPSPFGGLQVAVRLPLLRSRG"
     gene            785..1468
                     /locus_tag="PLS34_12610"
     CDS             785..1468
                     /locus_tag="PLS34_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRP88341.1"
                     /translation="MNTAQEILIVEDEPKLAALLQDYLHAAGFSTRVIEDGRKVVPAV
                     RAAPPALLLLDLMLPGRDGLEICRELRGFSRLPIIMMTARVEEIDRLLGLELGADDYI
                     CKPYSPREVVARVKAVLRRLLDSSAPPAGGVAGLAIEPESYRAALDGRVLDLTPVEFR
                     LLETLASHPGRVFSRAALLERLYDDHRIVSDRTVDSHVKNLRRKLGEAAPGRELIQSV
                     YGVGYRLED"
     gene            1590..>2123
                     /locus_tag="PLS34_12615"
     CDS             1590..>2123
                     /locus_tag="PLS34_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRP88342.1"
                     /translation="MTSRKLTTTLLAVAATALSFSALAQTPEAGQPEFRDGRGGGMLQ
                     RLDADGDGVVSWQEFQAAGAARFTALDADGDGRISAEEFAAGRRGPARAAADKADGRQ
                     AGPRAERMQQFRAQHFAKLDADGDGYVSRAEFDASHTARFNALDVNGNGAIDADEQPA
                     RRGMRDGKGRGDGCAKGG"
BASE COUNT          307 a          776 c          752 g          288 t
ORIGIN      
        1 ccggcgattt cagcgcgcgg gcgcacgccg ggtccagcga cgagttcggc cggctggcgg
       61 ccgacttcaa cctgctggcc cgcaccctcg agcgcaacga ggccatgcgc cggcgcatga
      121 tggccgacgt ggcccacgag ctgcgcacgc cgctggcggt cgtgcagggc gagctcgccg
      181 ccatcgagga cggcatccgg cccttcgctg cggaaaccct tgcctcgctg caggcggaga
      241 cccgcgcgct gggcaagctc atcgacgacc tctaccagct gtccctgtcg gacgtcggcg
      301 cgctcgatta ccgtcgtcgc gacctcgacc tgcgcgtgct ggtgggagag gcgctccagc
      361 cgctgcgcga gcgctgcgcg gccgccggcc tgtcgctcga cgacgaggcc gtggccgggg
      421 cgccgctgtc ggtgcacgcc gaccccgacc ggctggtcca actggtcgcc aacctcgtcg
      481 agaacgcgat ccgctatacc gatgcacccg gccgcatcgt ggtggcgtgc cgacaggcag
      541 aagggcacgc cgtgctcgag gtgagcgaca ccgcgccggg cgtgccaccc gagctgcagc
      601 cacagctgtt cgagcgcttt ttcagggtgg acacctcgcg cagccgggcc accggcggcg
      661 ccgggctcgg gctggccatc tgccgcaaca tcgtcgaggc gcacaacggc acgatcacca
      721 ccgcgccctc gccgttcggt ggcctgcagg tcgcggtgcg gctgccgctg ctcaggagcc
      781 gcggatgaac acggcccagg agatactcat cgtcgaggac gagccgaagc tcgcggcact
      841 gctgcaggac tacctgcacg ccgccggctt cagcacgcgg gtgatcgagg acgggcggaa
      901 agtcgtgccc gcggtgcgcg cggcgccgcc cgcgctgctg ctgctcgatc tcatgctgcc
      961 cgggcgcgac ggcctcgaga tctgtcgtga gctgcgcggt ttcagccggc tgcccatcat
     1021 catgatgacc gcccgggtgg aggaaatcga ccggctgctg ggcctggaac tcggcgcgga
     1081 cgactacatc tgcaagccct acagtccgcg cgaggtggtg gcgcgcgtca aggcggtact
     1141 gcggcgcctg ctcgactcgt cggcgccgcc ggccggcggc gtggcgggcc tcgccatcga
     1201 gcccgaatcc taccgcgccg ccctggacgg ccgggtgctg gacctgacgc cggtggaatt
     1261 ccggctgctg gagacgctgg cctcccatcc cgggcgcgtg ttctcgcgcg ccgcgctgct
     1321 cgagcgcctc tacgacgacc accgcatcgt ctcagaccgc accgtggaca gccacgtgaa
     1381 gaacctgcgc cgcaagctgg gcgaggccgc gcccgggcgc gaactgatcc agtcggtcta
     1441 cggcgtgggc taccggctcg aggactgagc agcgcgcaat ctccacgctt tctccgcatt
     1501 gctgccacgc tcgctgcacc ggcccggcgc aagctttcat caacgccggg gctcccggcg
     1561 gccgatgaac ggaatgcagg agagatgcca tgacttcgag gaaattgacc acgaccttgc
     1621 tggccgttgc ggccaccgcc ctgtccttct ccgccctggc gcagaccccc gaggccggcc
     1681 agcccgagtt ccgcgacggc cgtggcggcg ggatgctgca gcggctcgat gccgacggtg
     1741 acggcgtggt gtcatggcag gaattccagg ccgccggcgc ggcacgcttc accgcgctcg
     1801 atgccgacgg cgacggccgc atcagtgccg aagagttcgc cgccggccga cgcgggccgg
     1861 cccgcgccgc ggccgacaag gccgacggcc ggcaagcggg cccgcgcgcc gagcgcatgc
     1921 agcagttccg tgcgcagcat ttcgccaagc tggacgcgga cggcgacggt tacgtcagcc
     1981 gggccgagtt cgacgcatcg cacacggcac gcttcaacgc cctcgacgtg aacggcaatg
     2041 gcgcgatcga cgccgacgag caacccgccc gtcgcggcat gcgcgacggt aagggccggg
     2101 gcgacggttg cgccaagggc ggg
//
LOCUS       DAOMPG010000326         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20431_length_2118_cov_3.712070, whole genome shotgun sequence.
ACCESSION   DAOMPG010000326 DAOMPG010000000
VERSION     DAOMPG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20431_length_2118_cov_3.712070"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLS34_12620"
     CDS             <1..693
                     /locus_tag="PLS34_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRP88343.1"
                     /translation="AGERLGARALVGEPTGLKPINRHKGVLMERIRITGRSGHASDPA
                     AGLNAIDGAMAAITALHDFVDHLAAELPDLGFPVPGPTVNVGAIRGGDNPNRICASCE
                     LDVDLRLVPGMTLASARQGLRNGVTRMLEGSGFALEFVPLFAGVEPLAPRDGHALAKA
                     CEQAAGHACGAVNFGTEGPLYQQLDMEAVICGPGEIAVAHQPDESIELAEAARAVDMV
                     EALIRRFCASPA"
     gene            690..2057
                     /locus_tag="PLS34_12625"
     CDS             690..2057
                     /locus_tag="PLS34_12625"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRP88344.1"
                     /translation="MSARRDANAELVARDLARVWHPCTQMKDHEWLPLVPIRRGAGAW
                     LEDFNGRRYLDAISSWWVNLFGHANPVIGNAVRRQLETLEHVMLAGFTHEPVVELSER
                     LVAVTPAGLERCFYADNGSSAVEVALKMSFHYWLNQGESKRKRFVALENSYHGETLGA
                     LAVGDVALYKDTYRPLLMDCLLAPSPDAWLREAGESPAGFGRRRAAELEALLERHPGE
                     VCAVIVEPLVQCAGSMRMHDAAFLTELRAVCDRHRVHLIADEIAVGFGRTGTMFACEQ
                     AGIAPDFLCLSKGLTGGYLPLSVVLTSNAVYDAFYDDYATLRAFLHSHSYTGNPLACA
                     AALATLDLFAASDVLAGNRARAAQMRAGVAELEDHPHVGEIRQAGMILAIEMVEDRAS
                     RRPYRWQERRGLKVYQHGLASGVLLRPLGPVVYFMPPYVVDEEDIALMTRTAVEGVRL
                     ATEAD"
BASE COUNT          297 a          752 c          764 g          305 t
ORIGIN      
        1 gccggcgagc ggctcggcgc gcgcgcgctg gtgggcgagc caaccggact caagccgatc
       61 aaccgccaca agggcgtgct catggagcgc atccgcatca ccgggcgcag cggccacgcc
      121 agcgacccgg cggccgggct gaacgccatc gacggcgcca tggcggcaat caccgcgctg
      181 cacgacttcg tggaccacct cgcggccgag ctgcccgacc tcggcttccc cgtgccgggc
      241 ccgacggtca acgtcggcgc catccgcggc ggcgacaacc ccaaccgcat ctgcgcgagc
      301 tgcgagctgg acgtggacct ccggctggtg ccgggcatga cgctggcctc cgcccgccag
      361 ggcctgcgca acggcgtcac gcgcatgctc gagggcagcg gcttcgcact ggagttcgtg
      421 ccgctgttcg cgggcgtgga gccgctggcg ccgcgcgacg ggcacgccct cgcgaaggcc
      481 tgcgaacagg ccgcgggcca tgcctgcggc gcggtcaatt tcggcaccga gggcccgctc
      541 taccagcagc tcgacatgga agccgtgatc tgcggcccgg gcgagatcgc ggtcgcccac
      601 cagccggacg agtccatcga gctggccgag gcggcgcgcg cggtggacat ggtcgaggcg
      661 ctgatccggc gcttctgcgc aagcccggca tgagcgcgcg acgcgacgcc aacgccgagc
      721 tggtggcgcg cgacctggcg cgggtctggc atccctgcac gcagatgaag gatcacgagt
      781 ggctgccgct ggtgcctatc cggcgcggcg ccggcgcctg gctggaggac ttcaacggcc
      841 ggcgctacct cgacgccatc agctcctggt gggtgaacct gttcggccat gccaacccgg
      901 tcatcggcaa cgcggtgcgc cgccagctgg agacgctcga gcacgtcatg ctcgccggct
      961 tcacgcacga gccggtggtg gagttgtccg agcggctggt ggccgtcacc ccggcagggc
     1021 tcgagcgctg cttctatgcg gacaacggct cctccgcagt cgaggtcgcg ctgaagatga
     1081 gcttccatta ctggctcaac cagggcgagt cgaagcgcaa gcgcttcgtg gcgctggaga
     1141 acagctacca cggcgagacg ctcggcgcgc tcgcggtcgg cgacgtggcg ctctacaagg
     1201 acacctaccg gccgctgctg atggactgcc tgctggcgcc ctcgccggac gcctggctgc
     1261 gcgaggccgg cgagagcccg gccgggttcg gccggcggcg cgcggccgag ctggaggcgc
     1321 tgctcgagcg tcatcccggc gaggtctgtg cggtgatcgt cgagccgctg gtgcagtgcg
     1381 ccggcagcat gcgcatgcac gacgcggcgt ttctgaccga gctgcgcgcc gtctgcgacc
     1441 ggcatcgggt gcacctgatc gccgacgaga tcgcggtcgg cttcggccgc accggcacga
     1501 tgttcgcctg cgagcaggcc ggcatcgcgc cggatttcct gtgcctttcc aagggcctca
     1561 ccggcggcta cctgccgctc tccgtggtgc tcacctcgaa cgcggtctat gacgccttct
     1621 acgacgacta cgcgacgctg cgcgctttcc tgcattcgca cagctacacc ggcaacccgc
     1681 tggcctgcgc agcggcgctg gcgacactcg acctgttcgc ggccagtgac gtgctggcgg
     1741 gcaaccgcgc gcgcgcggcg cagatgcgcg ccggcgtggc ggaactcgag gaccatccgc
     1801 acgtcgggga gatccgccag gccggcatga tcctggcgat cgagatggtg gaggatcgcg
     1861 cctcgcgccg gccgtatcgc tggcaggaac gccgcggcct caaggtctac cagcacggcc
     1921 tcgccagcgg ggtgctgctg cgcccgctgg ggcccgtggt gtacttcatg ccaccctacg
     1981 tggtggacga ggaagacatt gcgctgatga cacgcaccgc ggtcgagggt gtgcgcctgg
     2041 cgacggaggc cgattgatgt cgcgcacgcc gcgggtgttc gtgcatgcgc cgctggccac
     2101 cgggcaacga ctcgtgct
//
LOCUS       DAOMPG010000327         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20831_length_2086_cov_4.177745, whole genome shotgun sequence.
ACCESSION   DAOMPG010000327 DAOMPG010000000
VERSION     DAOMPG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20831_length_2086_cov_4.177745"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..525)
                     /locus_tag="PLS34_12630"
     CDS             complement(85..525)
                     /locus_tag="PLS34_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRP88345.1"
                     /translation="MAITDRQMQAARTRAQELKTHVPQATGAAYNPDTGLVCIRLSNG
                     IVIGLPAAQTQGLETASTKNLSVIHISPSGYGIHFPELDVDLYLPALMEGIFGTKAWM
                     AERGRKGGQATTKAKKAAARENGKLGGRPRKTEKAAAQEKRGAA"
     gene            complement(512..760)
                     /locus_tag="PLS34_12635"
     CDS             complement(512..760)
                     /locus_tag="PLS34_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRP88346.1"
                     /translation="MATVLRFLSYRVAIYSNDHRPCHIHVMSAEHEAIFELNCPEGPP
                     SLRENYGFRKATLNLIAPHLQDNLADLCKQWKQIHGNY"
     gene            1223..>2086
                     /locus_tag="PLS34_12640"
     CDS             1223..>2086
                     /locus_tag="PLS34_12640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRP88347.1"
                     /translation="MARNNNEKNGNGSALGFEADLFKAADKLRGNMEPSDYKHVALGL
                     IFLKYISDAFEAKRKTLLAEDAQAAEDKDEYLADNVFWVPKEARWSHLQAGAKLPTIG
                     TLIDDAMRAIEKDNASLKGVLPKDYARPALNKVMLGELIDLISGIALGEAGDKSRDIL
                     GRVYEYFLGQFAGAEGKRGGEFYTPRSVVRVLVEMLEPHDGRVYDPCCGSGGMFVQSE
                     KFVAEHGGRIGDIAIYGQESNYTTWRLAKMNLAVRGIDSDIRWNNEGSFHKDELRDLK
                     ADYILANPPFNI"
BASE COUNT          370 a          635 c          662 g          419 t
ORIGIN      
        1 tgaggttggc ggcttcctcg gcggtgtcgg ccagcgcatc ccagacgctg gcgaaccgtg
       61 tcgtgatgcc attcatggga gttctcatgc tgccccgcgt ttttcttgtg cggcggcctt
      121 ttccgtcttc cgaggacggc cgccaagctt tccgttctct cgtgccgcgg ctttcttggc
      181 cttcgttgtt gcctggccac ccttgcgtcc gcgttctgcc atccaggcct tcgtcccgaa
      241 gatgccttcc atgagggcgg gcaggtagag atccacgtcg agctccggga agtggatgcc
      301 gtacccgctg gggctgatgt ggatcaccga caggttcttg gtgctggcgg tctccagccc
      361 ctgtgtctgc gctgccggca atccgatcac gatcccgttg gacaggcgga tgcacaccag
      421 gccggtgtcc gggttgtagg ctgcgcccgt cgcctgtggc acatgggttt tgagttcctg
      481 agcgcgggtg cgggctgcct gcatttgacg gtcagtaatt gccatggatc tgtttccact
      541 gcttgcatag gtcagccagg ttgtcctgca gatgcggcgc gatcaggttc agcgtggcct
      601 ttctgaaccc gtagttctcc ctcagagagg gcgggccttc cgggcagttc aactcgaaga
      661 tggcctcgtg ctcggcgctc atgacgtgga tgtggcaagg gcggtgatcg ttcgagtaga
      721 tggcgacccg gtagctcagg aacctcagga cagtagccat ggtatcaaaa acctaagccg
      781 tttgggtttt ccggtttcgg ccaatcgtgg tcggctcagg atcgggcttt gctgccggat
      841 gttccggtac ctgctccggc cggaggtggg tgttccgctt caaggtcatc caggactcgt
      901 gctgggtggc ttggggaatg gctctgaagt cagtgtcggc atccggtcgg gcagctccat
      961 cggacaacca ctgcaaacga tgtcggagaa ctgcgcggca gcggcaggag tcgaattcgt
     1021 acaatgcgcc tcccataccc gcctccaccg ccattcccgc cgaacaactg cgcctctccg
     1081 tcgcgccgat gatggattgg actgcacatt gaaaaacatg cgtgcaggtc aatagcttgc
     1141 gggcctttga gttggatatg ttgcaatctt ggtgcacacg gcttttgctg tggcgcggca
     1201 ctaccaacag gaacaggggc caatggcacg caacaacaac gaaaagaacg gcaacggcag
     1261 cgccctcggc ttcgaggctg atcttttcaa ggccgccgac aagctgcgcg gcaatatgga
     1321 gccgtccgac tacaagcacg tcgcgctcgg gctgatcttc ctgaagtaca tctccgacgc
     1381 cttcgaagcc aagcgcaaga cgctgctcgc ggaagacgcg caggctgccg aggacaagga
     1441 cgaatacctc gccgacaacg tgttctgggt gccgaaagag gcgcgctggt cgcatctgca
     1501 ggcaggcgcc aaattgccca ccatcggcac gctgatcgac gacgccatgc gcgccatcga
     1561 gaaggacaac gcgtcgctca agggcgtgct gcccaaggac tacgcccggc ccgcgctcaa
     1621 caaggtgatg ctgggcgagc tgatcgacct gatctccggc attgcgctgg gcgaggccgg
     1681 cgacaagtcc cgcgacatcc tcggccgcgt gtacgagtat ttcctcggcc agttcgccgg
     1741 ggccgaaggc aagcgcggcg gcgagttcta caccccgcgc tcggtggtgc gggtgctggt
     1801 tgaaatgctg gaaccgcacg atggccgcgt ctacgaccct tgctgcggct cgggcggcat
     1861 gttcgtgcag tcggaaaagt tcgtggccga gcacggcggc cgcatcggcg acatcgcgat
     1921 ttatgggcag gaatcgaact acaccacctg gcggctggcc aagatgaact tggctgtgcg
     1981 cggcatcgac tccgacatcc gctggaacaa cgagggcagc ttccacaagg acgagctgcg
     2041 cgacctcaag gccgactaca tcctcgccaa cccgccgttc aacatc
//
LOCUS       DAOMPG010000328         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_20977_length_2075_cov_4.039109, whole genome shotgun sequence.
ACCESSION   DAOMPG010000328 DAOMPG010000000
VERSION     DAOMPG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20977_length_2075_cov_4.039109"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..683
                     /gene="coaE"
                     /locus_tag="PLS34_12645"
     CDS             27..683
                     /gene="coaE"
                     /locus_tag="PLS34_12645"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670930.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRP88348.1"
                     /translation="MRAPAFTDGRFRVALTGGIASGKSTVARLFAEQGVPVIDLDQVA
                     REVVEPGEPALAAIVELFGPEILAADGRLDRAALRTRVFRDPVARKRLEELLHPRILL
                     AAVRHAQLAGGPYQVIVAPLLVEFGLAGWMDRVLVVDCPAEAQLERLLARDGGDEAIA
                     RAILASQATREQRLAAAHDVIVNDGPPERLPAAVQTLHALYREMATGAERGGPGLRLP
                     "
     gene            731..1534
                     /gene="zapD"
                     /locus_tag="PLS34_12650"
     CDS             731..1534
                     /gene="zapD"
                     /locus_tag="PLS34_12650"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HRP88349.1"
                     /translation="MNRSVANAPARQEARETVVYEQPLTERMRTFLRIEFLARQAQHH
                     ARQPSAWSSRAAMGSLIEILAIVSRGDVRGEVLKDLERHATMLSGFASRPGVDRTRLD
                     ALLERISQLRHQLSSCGANYTQPLKDSEFLASIRHRSAIPGGTCEFDLPDFKHWLSLP
                     EEQRYSDFQAWFRHLKPLCDAIGQLLWLTRESSPGRPEIAPGGMFQHQIDRSIGCQLV
                     RISLPATANCYPETSGGQHRFTVRFLTWAGPEQRPVQVSGDVEFELTCC"
     gene            1543..1749
                     /gene="yacG"
                     /locus_tag="PLS34_12655"
     CDS             1543..1749
                     /gene="yacG"
                     /locus_tag="PLS34_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRP88350.1"
                     /translation="MREVSCPHCGKPVEWSPASKWRPFCSERCRLIDLGQWFGEEHAI
                     PGEDAGDVEGALGPGDDPESAGSR"
BASE COUNT          330 a          732 c          694 g          319 t
ORIGIN      
        1 gcacttcgcc ggactcgact gaggtgctgc gcgcccccgc cttcacggac gggcgctttc
       61 gcgtcgcgct gaccggcggc atcgccagcg gcaagtccac cgtggcgcgg ctgttcgccg
      121 agcagggcgt gccggtgatc gatctggacc aggtggcgcg cgaagtggtc gagccgggcg
      181 agccggcgct ggcggccatc gtcgagctgt tcggcccgga aatactcgcc gccgacggcc
      241 ggctggaccg cgcggcgctg cgcacccgcg tgttccgcga cccggtcgcg cgcaagcggc
      301 tcgaggaatt gctgcacccg aggatcctgc tggcggcggt gcgtcatgcc cagctcgccg
      361 gcggccccta ccaggtgatc gtcgccccgc tgctggtcga gttcggcctg gccggctgga
      421 tggaccgggt gctggtggtg gactgtcccg ccgaagccca gctcgagcgg ctgctggcgc
      481 gcgacggcgg cgacgaggcg attgcgcggg ccatcctcgc cagccaggcc acccgcgagc
      541 aacgtctcgc cgcggcccac gacgtgatcg tcaacgacgg cccgccggag cgcctgccgg
      601 ccgcggtgca gacgctgcat gccctgtacc gcgaaatggc caccggcgcc gaacgggggg
      661 gcccggggct gcgcctgccc tgagcgcacg ggggtttgcg gtgctgcgca gcatcgtaca
      721 gaatcgcgcg atgaaccgaa gcgtggcgaa tgcccccgcc cgacaggaag cacgggagac
      781 ggtcgtctac gagcaaccgc tcacggagcg gatgcggacc ttcctgcgca tcgagtttct
      841 cgcccgccag gcgcaacacc acgcccgcca gccctcggcc tggtcgagcc gcgccgccat
      901 gggcagcctg atcgagattc tcgccatcgt cagccgcggc gacgtgcgcg gcgaagtgct
      961 caaggacctc gagcggcacg ccaccatgct gtcgggcttc gccagccggc ccggcgtgga
     1021 ccgcacgcgc ctcgacgcat tgctggaacg catctcgcaa ctccgtcacc agctcagcag
     1081 ctgcggcgcc aactacaccc agcccttgaa agactccgaa ttccttgcct cgatccgcca
     1141 ccgcagcgcc atccccggcg gcacctgcga gttcgacctg ccggacttca agcactggct
     1201 gagcctgccc gaggagcaac gctacagcga cttccaggcg tggttccgcc acctcaagcc
     1261 gctgtgcgac gccatcggcc agctgctgtg gcttacccgc gaatcctcgc ccgggcgccc
     1321 cgagatcgcc ccgggcggca tgttccagca ccagatcgac cgctccatcg gctgccagct
     1381 ggtgcgcatc tcgctgcccg cgaccgccaa ctgctacccc gagaccagcg gcggccagca
     1441 ccgcttcacc gtacgcttcc tcacctgggc cggccccgaa cagcggccgg tgcaggtcag
     1501 cggcgacgtc gagttcgagc tgacctgctg ctgagcccgg caatgcgcga agtttcctgt
     1561 ccccactgcg gcaagccggt cgagtggtcg cccgcgtcca agtggcgtcc cttctgcagc
     1621 gagcgctgcc gcctgatcga cctcggccag tggttcggcg aggagcacgc catcccgggg
     1681 gaggatgcgg gcgacgtcga aggagcgctg ggtccgggcg atgaccctga atccgcgggg
     1741 agcaggtaga ttttcaagct tcccggctct ttcaacgtgg ctgtagacta agtcgagtcc
     1801 gagaacaagg ggcggttgcc atgtcgcgtg cgttcacaag gctcgagaag gaaatccagg
     1861 cgcttagcat cgaggagaag cgagagctcc tgcgggtgct cgtcgcagat ctcgataaat
     1921 tctcggagcc gcatattgaa aaagcctggt tggaagcagc ccagcgcaga cgtcacgagc
     1981 ttgcgtcggg tcaatcggag ggcgtacctg gtgctctcgt gttcgatcga attcactcac
     2041 gcttcggacg atgaactatg aatttcatcc cgagg
//
LOCUS       DAOMPG010000329         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_21022_length_2071_cov_4.213294, whole genome shotgun sequence.
ACCESSION   DAOMPG010000329 DAOMPG010000000
VERSION     DAOMPG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21022_length_2071_cov_4.213294"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /gene="uvrA"
                     /locus_tag="PLS34_12660"
     CDS             <1..>2071
                     /gene="uvrA"
                     /locus_tag="PLS34_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRP88351.1"
                     /translation="VESLSAYARQFLSMMEKPDVESIEGLSPAISIEQKSTSHNPRST
                     VGTVTEIYDYLRLLYARAGTPRCPTHAERLEAQTVSQMVDQVLALPEGTALMLLAPVV
                     RDRKGEHTSVMAELRAQGFIRARVDGEVCELDDPPSLDLHRKHTIEAVVDRFRVRPDL
                     GLRLAESFETALKLADGMATVAFMDEPKTPPLVFSDRFACPVCGYSIPELEPRLFSFN
                     NPAGACATCDGLGVQQFFDPERVAAHPHLSLAGGAIRGWDRRNAYYFQLVNSLAEHYQ
                     FDVETPFEQLPAKIRKVVLFGSGSETIEFAYFDGRGGKSRRKHRFEGIIPNMERRYRE
                     TESGTVRDELSKYLGTHPCPSCKGTRLNEAARHVFVAERGLADVTATAVGAALDFFSA
                     LELPGWRGEVARKIVKEIKDRLKFLVDVGLDYLTLDRSAETLSGGEAQRIRLASQIGS
                     GLVGVMYILDEPSIGLHQRDNRRLLNTLVHLRDLGNTVIVVEHDEEAIATADHIVDIG
                     PGAGVHGGRIVAQGAPADIMASPESLTGDYLAGRRGIAVPAARTPRRKGREIVVRGAR
                     GNNLAGLDVVIPLGLLTCVTGVSGSGKSTLINDTLYLAAAQKLNGAAVTPAPHDRIEG
                     LQHIDKVIDISQSPIGRTPRSNPATYSGLFTPIRELFAGTAESRSRGYKAGRFSFNVK
                     GGRCEACQ"
BASE COUNT          335 a          748 c          681 g          307 t
ORIGIN      
        1 gtggagtcgc tgtcggccta tgcccggcag ttcctgtcga tgatggaaaa accggacgtg
       61 gagtccatcg aggggctctc gcccgccatt tccatcgagc agaagtccac ttcgcacaat
      121 cctcgctcca ccgtcggcac ggtcaccgag atctacgatt acctgcggtt gctctatgcc
      181 cgcgccggca cgccgcgctg ccccacccac gccgagcggc tggaggccca gaccgtcagc
      241 cagatggtcg accaggtgct ggcgctgccg gagggcactg ccctgatgct gctcgcgccg
      301 gtggtgcgcg accgcaaggg cgagcacacc agcgtgatgg ccgagctgcg cgcgcagggc
      361 ttcatccgcg cgcgggtgga cggcgaggtc tgcgagctgg acgacccgcc gtcgctcgac
      421 ctgcaccgca agcacaccat cgaggcggtg gtagaccggt tccgggtgcg gcccgacctg
      481 ggcctgcgcc tggccgagtc cttcgaaacc gcactgaagc tcgccgacgg catggcgacg
      541 gtcgcgttca tggacgagcc gaagacgccg ccgctggtat tctccgaccg cttcgcctgc
      601 ccggtgtgcg gctacagcat cccggaactc gagccgcggc tgttctcctt caataacccg
      661 gcaggcgcct gcgccacctg cgacggactc ggcgtgcagc agttcttcga tccggagcgg
      721 gtggcggccc atccgcacct gtcgctggcc ggcggcgcca tccgcggctg ggaccggcgc
      781 aacgcctatt acttccagct ggtcaacagc ctcgccgagc actaccagtt cgacgtcgag
      841 acgcccttcg agcagctgcc ggcgaaaatc cgcaaggtgg tgctgttcgg ctccggcagc
      901 gagaccatcg agttcgccta cttcgacggc cgcggcggca agagccggcg caagcaccgt
      961 ttcgaaggca tcattcccaa catggagcgc cgctaccgcg agaccgagtc cggtacggtg
     1021 cgcgacgagc tctccaagta cctcggcacc cacccctgcc cgagctgcaa gggcacgcgg
     1081 ctgaacgagg cggcgcgcca tgtgttcgtc gccgagcgcg gcctcgcgga cgtcaccgcc
     1141 accgccgtcg gcgcggcgct ggactttttc agcgccctcg aactgcccgg ctggcgcggc
     1201 gaagtcgccc gaaaaatcgt caaggaaatc aaggaccgct tgaagttcct ggtggatgtc
     1261 gggctggact acctgacgct cgaccgcagc gccgagacgc tgtccggcgg cgaggcccag
     1321 cgcatccgcc tggccagcca gatcggctcg gggctggtgg gcgtcatgta tatcctcgac
     1381 gagccctcca tcggcctgca ccagcgcgac aaccggcggc tgctgaacac gctggtgcat
     1441 ctccgcgacc tgggcaacac ggtgatcgtg gtggagcacg acgaggaagc catcgccacc
     1501 gccgaccaca tcgtcgatat cggtcccggc gccggcgtgc acggcgggcg catcgtggcg
     1561 cagggcgcac cggccgatat catggccagc cccgagtcgt tgaccggcga ctatctcgcc
     1621 ggccggcggg gcatcgccgt ccccgccgcc cgcacgccgc gcaggaaagg ccgcgagatc
     1681 gtcgtccgtg gcgcgcgcgg taacaacctg gccgggctcg acgtggtcat cccgctcggc
     1741 ctgttgacct gcgtgaccgg cgtgtcgggc tccggcaagt cgacgctcat caacgacacc
     1801 ctgtacctgg ccgcggcaca gaagctcaac ggtgcggccg tcacgcccgc tccgcacgac
     1861 cgtatcgagg gcctgcagca catcgacaag gtcatcgaca tcagccaaag cccgatcggc
     1921 cgtacgccgc gctccaaccc ggcgacctac agcggcctgt tcacgccgat tcgcgagctg
     1981 ttcgcgggca cggccgagtc acgctcgcgc ggctacaagg cggggcgctt cagcttcaac
     2041 gtcaagggcg gccgctgcga ggcctgccag g
//
LOCUS       DAOMPG010000330         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_21201_length_2056_cov_4.611694, whole genome shotgun sequence.
ACCESSION   DAOMPG010000330 DAOMPG010000000
VERSION     DAOMPG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21201_length_2056_cov_4.611694"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLS34_12665"
     CDS             complement(<1..973)
                     /locus_tag="PLS34_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88352.1"
                     /translation="MGAQTPGRDPVAGDFRERWVQLALPLLGIVLLMLFLVLGRPPSD
                     SYGWRTAFELGHVPLFGVTGLLMLRVFRVLRGAAAPDGTVFLLASLSMAVLGLAAEAT
                     QVLQAGRNANAGDAMLNLTGALCFLAIAATLRPGMWRRLGRDGPLAARLVLAGALLAL
                     VLVSLPLLGVAWSYGQRAAAFPVLADLTQRWQKPFLSVGRSELATVRAPAGWTDRAGT
                     QVARLTFLDAPWPGFTVREPWPDWTAYDALRFQVWSDMDAPVEIVLRVDDTHRDRPHS
                     DRFNGSFIVMPGVNDFTVPLDTIASGPRDRELDLADISQFVLFSRRPG"
     gene            1081..1803
                     /locus_tag="PLS34_12670"
     CDS             1081..1803
                     /locus_tag="PLS34_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase-C39 like family protein"
                     /protein_id="HRP88353.1"
                     /translation="MSPHPFLDIRMLPQPDDVTCGPTCLQAVYRHLGHPLELEQIIAE
                     VRGLPEGGTLAVYLGNHALKRGLKARLYSYHLKIFDPSWRGLPPGELAAKLEAQLAYK
                     HGKKFETASRAYIRFLELGGEIALDDLTSQVFDAPFNAGLPILTGLSATYLYDSRREY
                     TNRHNRAVYDDLRGEPTGHFVVLCGRERGKVRVADPYIGNPLADDSHYYDVRVNRLIR
                     AILLGVMTYDANLLVISDGDLP"
     gene            1800..>2056
                     /locus_tag="PLS34_12675"
     CDS             1800..>2056
                     /locus_tag="PLS34_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HRP88354.1"
                     /translation="MRRLIVVDDPKAWTLGGHDGDVVSVSDYLAPSEPWPSRGVRVFN
                     LARSYGYQTRGYYVSLLAEARGHRVMPDVRTIQDLNAPGFVR"
BASE COUNT          341 a          743 c          686 g          286 t
ORIGIN      
        1 cgccgggccg tcggctgaac agcacgaact ggctgatgtc ggcgagatcg agctcgcggt
       61 cgcgcggccc gctggcgatc gtatcgagcg gcacggtgaa gtcgttcaca cccggcatga
      121 cgatgaaact gccgttgaag cggtcgctgt gcggccggtc gcggtgcgtg tcgtccaccc
      181 gcaggacgat ctccacggga gcgtccatgt cggaccagac ctggaaccgg agggcgtcgt
      241 aggccgtcca gtcgggccac ggctcccgca ctgtgaaccc cggccatggc gcgtcgagaa
      301 aggtcaggcg tgccacctgc gtaccggcgc gatccgtcca gccggccggg gcgcggacgg
      361 tcgcgagttc ggagcgcccg acgctcagga agggcttctg ccaccgctgg gtgaggtctg
      421 ccagcacggg gaatgccgcg gcccgctggc cgtagctcca ggcgaccccg aggagtggca
      481 gcgacaccag caccagtgcc agcagcgcgc cggccagcac cagccgggcg gccagcggcc
      541 cgtcgcgtcc cagtcgtcgc cacatgcccg ggcgcagggt tgccgcgatg gccaggaagc
      601 acagcgctcc ggtcaggtta agcatggcgt ccccggcatt cgcattgcgg ccggcctgca
      661 gcacctgcgt ggcctcggcg gccaggccga gcacggccat cgacagcgac gccagcagga
      721 acaccgtgcc gtcgggggcc gccgctccgc gcagcacccg gaagacccgc aacatcagca
      781 ggcccgtcac accgaacagc ggcacgtgtc cgagctcgaa agccgtgcgc cagccgtaac
      841 tgtcgctggg tggccggccc agcacgagga acagcatcaa caggacgatg ccaagcagcg
      901 gcagcgccag ctgtacccag cgctcccgga agtccccggc caccggatcg cggcctggcg
      961 tctgcgcacc catgccggga ttctgcctgc gatcgtggcc cgcaagccag ccaacgcccg
     1021 gattgacccc tgcgcgaaaa ggcgcgagat tggcgcaccg gaacgacttg caggcccacc
     1081 gtgagccctc atcctttcct cgacatccgc atgctgccgc agccggacga cgtcacctgc
     1141 ggcccgacct gcctgcaggc ggtgtaccgc catctcgggc atccgctcga gctcgaacag
     1201 atcattgccg aagtgcgcgg actgccggaa ggcggcacgc tggcggttta cctcggcaac
     1261 catgcgctga agcgcggcct caaggcgcgc ctctacagct atcacctgaa gatcttcgac
     1321 ccctcctggc gcggcctgcc gccgggtgag ctggccgcga agctcgaagc gcagctggcg
     1381 tacaagcacg gcaagaaatt cgagactgcg agtcgcgcct acatccgctt cctcgagctc
     1441 ggcggcgaaa tcgcgctcga cgacctgacc tcgcaggtgt tcgatgcgcc cttcaatgcc
     1501 ggcctgccga tcctgaccgg gctgagcgcc acctatctct acgacagccg gcgcgagtac
     1561 accaatcgcc acaaccgcgc cgtgtacgac gacctgcgcg gcgagccgac cggccacttc
     1621 gtggtgctgt gcggccggga gcgcggcaag gtgcgtgtcg ccgacccgta catcggcaac
     1681 ccgctggccg acgacagtca ctactacgac gtccgggtca accgcctgat ccgcgcgatc
     1741 ctgctcggcg tgatgacgta cgacgccaac ctgctggtca tctccgacgg agacctgccg
     1801 tgagacgcct gatcgtggtg gacgacccga aggcctggac gctcggcggg cacgatggcg
     1861 acgtggtgtc ggtgagcgac tacctcgcgc cgtccgagcc ctggccgtcg cgcggcgtgc
     1921 gggtgttcaa cctggcgcgc tcctacggtt accagacccg cggctactat gtctcgctgc
     1981 tcgccgaggc gcgcgggcat cgcgtcatgc ccgacgtgcg caccatccag gacctgaacg
     2041 cgccgggctt cgtgcg
//
LOCUS       DAOMPG010000331         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_21206_length_2056_cov_3.493753, whole genome shotgun sequence.
ACCESSION   DAOMPG010000331 DAOMPG010000000
VERSION     DAOMPG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21206_length_2056_cov_3.493753"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLS34_12680"
     CDS             <1..740
                     /locus_tag="PLS34_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRP88355.1"
                     /translation="LLAQRHPGFGDLRVLAMALCRRTGATLGYLGEGANSAGACLAGV
                     LPHRAAGGSPVAAPGLDAAAMLAGGLDAMVLLGLEPEHDSAGGAAALEGLQRCGFVVC
                     LTPYATPAMKAYADVLLPVGTAYETSGTFVNCEGRRQSFAGAARPVGEARPAWKVLRV
                     LANQLGLADFEYQSSEDVRDALLAELGDRESGDLAGSPDVPLRAVALRHEGGVQAPGV
                     YATDMLLRRSRPLQDTAAGRAGGRRLA"
     gene            737..1783
                     /gene="nuoH"
                     /locus_tag="PLS34_12685"
     CDS             737..1783
                     /gene="nuoH"
                     /locus_tag="PLS34_12685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRP88356.1"
                     /translation="MIEFWTGLPAELQRTLIVTLQVVAVVVPLILTVAYLTLAERKVI
                     GWIQNRVGPNRVGPKGLLQPFADVLKLLIKEIVIPAKSSRFLFVIAPLLAIVPAFATW
                     AVIPVSPEFVLADINAGVLYVLALTSMGVYGIILAGWASNSKYALLGAMRSAAQIVAY
                     EIAMGFALVGVLMAGGSLNLGEIVRAQEGSVLHWFWLPLLPLFVIYFVSGVAETNRAP
                     FDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILIAALAATFFLGGWLSPFQGIPG
                     LDGTFLAAPGFVWFALKTSLFLFCFLWLRATFPRYRYDQIMRLGWKVFIPITIIWLFV
                     EGLMVWFGVGPWFS"
     gene            1783..>2056
                     /gene="nuoI"
                     /locus_tag="PLS34_12690"
     CDS             1783..>2056
                     /gene="nuoI"
                     /locus_tag="PLS34_12690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRP88357.1"
                     /translation="MNRLRNLARTLFLTELLAGLRLTLSNLFRRKVTLNYPEEKAPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESDVGENGLRR"
BASE COUNT          261 a          680 c          737 g          378 t
ORIGIN      
        1 ggctgctggc gcagcgccat ccgggtttcg gcgacctgcg cgtgctggcc atggcgctgt
       61 gccgccgcac cggcgcgacg ctgggctacc tgggcgaagg ggccaattcg gcgggggcct
      121 gcctggcggg cgtgctgccg catcgcgcgg ccggcggctc gccggtggct gcacccggac
      181 tggatgccgc agcgatgctt gcgggcgggc tggacgccat ggtgttgctc ggactggagc
      241 cggagcacga cagcgccggc ggcgcggcgg cgctcgaagg gctgcagcgc tgcggtttcg
      301 tcgtctgtct gacgccgtat gccacgcccg cgatgaaagc ctatgcggac gtgttgctgc
      361 cggtgggcac ggcctacgag acctccggca ccttcgtgaa ttgcgaaggc cggcggcagt
      421 cgttcgcggg cgcggcccgg ccggtcgggg aagcccggcc tgcctggaag gtgctgcggg
      481 tgctcgctaa ccagctcggc ctggcggact tcgagtacca gagctccgag gatgtgcgtg
      541 acgcgctgct ggcggaactg ggcgaccgcg agagtggcga cctggccggc agtccggacg
      601 tgccgctgcg tgcggtggcg ctgcgccacg agggcggggt gcaggcgccg ggcgtgtacg
      661 ccaccgatat gctgctgcgc cgctcgcgac ccctgcagga cacggctgcg ggccgcgcgg
      721 gcgggaggcg cctggcatga tcgagttctg gacgggactg ccggcggagc tgcagcggac
      781 cctgatcgtc acgctgcagg tggtggcggt ggtcgtgccg ctgatcctga cggtggctta
      841 cctgacgctg gccgagcgca aggtgatcgg ctggatccag aatcgcgtcg gccccaaccg
      901 ggtgggcccg aaggggctgt tgcagccctt tgcggacgtg ctcaagctgc tcatcaagga
      961 aatcgtcatc ccggcgaaat ccagccgctt cctgttcgtg atcgcgccgc tgctggcgat
     1021 cgtgccggcc ttcgcgacct gggcggtgat cccggtgagc ccggagttcg tgctggccga
     1081 catcaatgcc ggcgtgctct acgtgctggc gctgacctcg atgggcgtct acggcatcat
     1141 cctggcgggc tgggcctcga actccaagta cgcgctgctg ggcgcgatgc gctcggccgc
     1201 gcagatcgtg gcctacgaga tcgccatggg cttcgccctg gtcggcgtgc tgatggccgg
     1261 cggcagcctc aacctcggcg aaatcgtgcg cgcccaggag ggcagcgtgc tgcactggtt
     1321 ctggctgccg ctgctgccgc tgttcgtcat ttatttcgtc tccggcgtgg ccgagaccaa
     1381 ccgcgcgccc ttcgacgtcg ccgagggcga atccgagatc gtggccggct ttcacgtcga
     1441 gtattccggc atggccttcg cggtgttttt cctcgccgag tacgccaaca tgatcctgat
     1501 cgcggccttg gccgcgacct tctttctcgg cggctggctg tcgcccttcc agggcatccc
     1561 cggactggac ggcaccttcc tcgccgcgcc gggcttcgtc tggttcgcgc tcaagacctc
     1621 gctgttcctg ttctgcttcc tgtggttgcg ggccaccttc ccgcgttacc gctacgacca
     1681 gatcatgcgg ctgggctgga aggtcttcat tcccatcacc atcatctggc tgttcgtcga
     1741 gggcctgatg gtctggttcg gcgtcggtcc gtggttcagc tgatgaatag actgcgcaat
     1801 ctcgccagga cactgtttct caccgagctc ctcgccgggc tgcggctgac cctcagcaac
     1861 ctgttccggc gcaaggtcac gctgaattac ccggaggaaa aggcgcctca gtcgccgcgc
     1921 ttccgcggcc tgcacgcgct gcggcgctac ccgaacggcg aggagcgctg cattgcctgc
     1981 aagctgtgcg aggcggtctg cccggcgctg gccatcacca tcgagagcga cgtcggcgag
     2041 aacggcctca ggcgga
//
LOCUS       DAOMPG010000332         2000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_21921_length_2000_cov_4.576864, whole genome shotgun sequence.
ACCESSION   DAOMPG010000332 DAOMPG010000000
VERSION     DAOMPG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21921_length_2000_cov_4.576864"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLS34_12695"
     CDS             <1..302
                     /locus_tag="PLS34_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRP88358.1"
                     /translation="LGRVVPASTIGDPQSLAIRTRLNGTVMQESNTRLMLFPVATLVA
                     ELSRGTTLLAGTVVLTGTPAGVGAARKPPVFLQPGDVVEVEIERVGVLSNPVREA"
     gene            398..1069
                     /locus_tag="PLS34_12700"
     CDS             398..1069
                     /locus_tag="PLS34_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRP88359.1"
                     /translation="MQPARSTYAGVARPAVVETNNVLKNTYLLLAATLLFSAATAGIA
                     MFAGMPPLNPFIVLAGYFALLFATVKTRNSALGLVFVFALTGFMGLTLGPIISLYLTM
                     LPNGNEVVMTALGTTGATFVGLSAYAVKSKRDFSFLGGFLFVGILGAFLLGIFAYLFS
                     MPMLSLAVSGMFVIAMAGLILWQTSEIVRGGETNYIMATVTLYVSIYNLFTSLLHLFG
                     VLGDE"
     gene            1066..1692
                     /locus_tag="PLS34_12705"
     CDS             1066..1692
                     /locus_tag="PLS34_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP88360.1"
                     /translation="MSRKRAPARTLFALVAFGLVAAAAIYALAARDPTEVELASGIAL
                     PQPRKLPDFQLVNQHARSFGRADLEGGWTLLFAGFTHCPDICPTTLATLAAIDAQLRA
                     RGVALDTVFLSLDPERDTPATLAPYLAHFNPQFIGATGPQDEIARFMAGLGLAYIRVP
                     TGGGGYTIDHSAALVLIDPRARVAAYFKPPLVAERIAADLAALAGPRG"
     gene            complement(1715..>2000)
                     /locus_tag="PLS34_12710"
     CDS             complement(1715..>2000)
                     /locus_tag="PLS34_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88361.1"
                     /translation="AVVIEDALPYDEPPGWHMPVRHSLGAVLLDAGRPAEAEAVYREE
                     LRRNPENGWSLFGLTQSLLAQDDAAAAEAAGRRFERAWANADVELSASRL"
BASE COUNT          273 a          723 c          661 g          343 t
ORIGIN      
        1 cgctgggtcg cgtcgtgccg gccagcacga tcggcgatcc gcaatcgctg gccatccgca
       61 cccgcctcaa cggcacagtg atgcaggaga gcaacacgcg cctgatgctg tttccggtcg
      121 cgaccctcgt ggccgagctg tcgcgcggca cgaccttgct ggccggcacg gtcgtcctga
      181 cgggcacgcc ggcgggggtg ggcgccgccc gcaagccgcc ggtgttcctg cagcccggcg
      241 acgtggtgga ggtcgagatc gagcgggtcg gcgtgctgag caacccggtg cgcgaggcct
      301 gaaccgggcg cgcgcggccg cgggaacttc gtgccgcggc accggtcatg gtagtctgga
      361 gcctgattcc gaaccctttt ccggaggtcg attgcccatg caacctgccc gttcaacgta
      421 cgctggcgtg gctcgtccgg cggtcgtcga gaccaacaat gtcctgaaga acacctatct
      481 cctgctcgcg gcgacgctgc tgttcagcgc cgcgaccgcc ggcatcgcga tgttcgcggg
      541 catgccgccg ctcaatccct tcatcgtgct ggccggctac tttgcgctgc tgttcgccac
      601 ggtgaagacg cgcaacagcg cgcttggcct ggtgttcgtg tttgcgctga ccggcttcat
      661 gggcctcacc ctcgggccga tcatcagcct gtacctgacc atgctgccga acggcaacga
      721 ggtggtgatg accgcgctcg gcaccaccgg cgcgaccttc gtcgggctgt ccgcctatgc
      781 ggtgaagagc aagcgcgact tcagcttcct cggcggcttc ctgttcgtcg gcatcctggg
      841 cgccttcctg ctcggcatct tcgcctacct gttcagcatg ccgatgctgt ctctggcggt
      901 gtccggcatg ttcgtaatcg ccatggccgg gctgatcctg tggcagacca gcgagatcgt
      961 gcgcggcggc gagaccaact acatcatggc caccgtcacg ctgtacgtca gcatctacaa
     1021 cctgttcacc agcctgctgc acctgttcgg agtgctcggc gacgagtgag ccggaagcgc
     1081 gcgccagccc ggacgctgtt tgcgctcgtc gcgttcgggc tggtggcggc cgctgcgatc
     1141 tacgcgctgg ccgcacggga cccgacggag gtcgagctgg ccagcggcat cgcgttgccg
     1201 cagccgcgca agctgcctga cttccagctg gtcaaccagc atgcgcgatc cttcgggcgc
     1261 gccgacctgg aaggtgggtg gaccctgctg tttgccggct tcacgcactg ccccgacatt
     1321 tgcccgacga ccctggcgac gctcgccgcc atcgatgcgc aactgcgcgc acgcggcgtg
     1381 gcactcgaca cggtcttcct gagcctcgac cccgagcgcg acacgccggc cacgctggcg
     1441 ccctacctgg cacacttcaa tccgcagttc atcggcgcca ccggcccgca ggacgaaatc
     1501 gcgcgattca tggccgggct cgggctggcg tacatccggg tgccgaccgg tggcggtggc
     1561 tacaccatcg accattcggc agcgctggtg ctgatcgatc cgcgcgcccg cgtggcggcc
     1621 tatttcaagc cgccgctcgt cgccgaacgc atcgctgcgg acctggctgc cctggccggc
     1681 ccgcggggct gagcggtcgg cggttcgccg ccgctcagag ccgcgatgcg ctcagttcca
     1741 cgtccgcatt ggcccaggcg cgctcgaagc gccgcccggc cgcctcggcc gcggcagcgt
     1801 cgtcctgcgc cagcagcgac tgggtgaggc cgaacaggga ccagccgttc tcgggattgc
     1861 gccgcaactc ctcccggtag accgcctctg cctccgccgg cctgccggca tccagcagca
     1921 ccgcgccgag ggagtgccgc accggcatgt gccagcccgg cggttcgtca tagggcagcg
     1981 cgtcctcgat cacgaccgct
//
LOCUS       DAOMPG010000333         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_21944_length_1998_cov_4.471436, whole genome shotgun sequence.
ACCESSION   DAOMPG010000333 DAOMPG010000000
VERSION     DAOMPG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21944_length_1998_cov_4.471436"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLS34_12715"
     CDS             complement(<1..255)
                     /locus_tag="PLS34_12715"
                     /inference="COORDINATES: protein motif:HMM:NF020136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HRP88362.1"
                     /translation="MTPPLLLDDFHRDDGIAVLGTRWEGFTDRVMGGRSDMQVGYRDT
                     DVGRVLQMRGQVRLENRGGFIQARLPLATGGGSIDASEWQG"
     gene            complement(252..752)
                     /locus_tag="PLS34_12720"
     CDS             complement(252..752)
                     /locus_tag="PLS34_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88363.1"
                     /translation="MALALVAMPWTAPAAAASADAHDHTALRIAYTPRVFHFTSNKDY
                     VDWNHIVAVEYVTPRHTLWGADRSHFGLSLFDNSYGQFSQSIYYGLEWDWRPALGGQL
                     FFSVSPGLVHGYKEPYENRVPLNKTLGVGVTIVPAIGWERERLGFALSLVGSAVGIRG
                     SWTFGR"
     gene            complement(764..1234)
                     /locus_tag="PLS34_12725"
     CDS             complement(764..1234)
                     /locus_tag="PLS34_12725"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRP88364.1"
                     /translation="MLSPLNPAGSEGPTQSGVWLLDGHNLLYACRRAFAGQLADGHPG
                     TVARAELVRRLVTARPEPGPALFVYFDGAEPNTHSPAKHLQVVYSGGDGDQRADDAIL
                     RHVNAHRADAPDTPIVVVTRDIRLARRARKRGANVIDPGEFLQAWQLDEVDAHE"
     gene            complement(1439..>1998)
                     /locus_tag="PLS34_12730"
     CDS             complement(1439..>1998)
                     /locus_tag="PLS34_12730"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRP88365.1"
                     /translation="AAGAYDADAKKDKTKEKEMRSEQTMTDQARDTANDIRMHLALET
                     KFAQSDELSALAISTDVTHGVVHLKGDVETDARKEMATELARSVDGVKSVKNELRVTG
                     GEPGMLERMQATAGDAALSTRVKTRLLASSNTSGLAINVSTDDKIVMLEGEVDSETEK
                     ELAELIAANTSGVADVRNQLRVSNN"
BASE COUNT          291 a          641 c          695 g          371 t
ORIGIN      
        1 gccctgccac tcgctggcgt cgatgctgcc gccgcccgtt gcgagcggca gccgggcctg
       61 gatgaagccg ccgcgattct cgaggcgcac ctggccgcgc atctgcagca cccggccgac
      121 gtcggtgtcg cggtagccga cctgcatgtc ggagcggccg cccatgacac ggtcggtgaa
      181 gccttcccag cgcgtgccga gcacggcgat gccgtcgtcg cggtggaagt cgtcgagcag
      241 caggggtggg gtcatcgccc gaacgtccag ctgccgcgaa tcccgactgc ggagccgacc
      301 aggctgagcg cgaacccgag gcgttcgcgt tcccagccaa tggccgggac gatggtgacg
      361 ccgacgccga gcgtcttgtt gagcggcacg cggttttcgt agggctcctt gtagccgtgc
      421 accaggccgg ggctgacgga gaagaacagc tgcccaccca gcgccggccg ccagtcccat
      481 tcaaggccgt agtagatgga ttgcgagaac tggccgtagg agttgtcgaa cagcgacagg
      541 ccgaaatggc tgcggtcggc gccccacaac gtgtgccgcg gggtgacgta ctccaccgcg
      601 acgatgtggt tccagtcgac gtagtccttg ttgctggtga agtggaacac gcgcggcgtg
      661 taggcgatgc gcagcgcggt gtgatcgtgt gcgtccgcgc ttgcagcggc ggctggagcg
      721 gtccacggca ttgcgacgag cgccagtgcc atggcggcgg tcgctattcg tgcgcgtcca
      781 cttcgtccag ttgccatgcc tgcaggaatt cccccggatc gatcacgttt gcgccgcgct
      841 tgcgggcccg gcgcgccagc cggatgtcgc gggtgaccac gacgatcggc gtgtcggggg
      901 cgtcggcgcg gtgcgcgttg acgtgtctca ggatggcgtc gtccgcgcgc tggtcgccgt
      961 cgccgcccga gtagacgacc tggaggtgtt tcgcggggga gtgcgtgttg ggttcagcgc
     1021 cgtcgaagta gacgaacagc gccggccccg gttcagggcg tgccgtcacc aggcggcgca
     1081 ccagctcggc gcgcgccacg gtgcccgggt ggccgtccgc gagttgcccc gcgaacgccc
     1141 gccggcacgc gtacagcagg ttgtggccgt cgagcagcca tacccccgac tgggtcggac
     1201 cctcggaacc cgctggattt aaaggagaaa gcacttgaaa aaacggcctt tgggatcgtt
     1261 agaggacgga ttttcgtccg aaatcggtgc gctaaggatc cgacgagcgc aggggatgtt
     1321 tccgttagag gtgccattct ggcccgaaaa ccgtccccta agcactcgaa tgcggctgtt
     1381 tttcgactag tctcttttaa aaacaggtac ctatccgggt ccgacccgcg gggtgggctc
     1441 agttgttgct gacgcgcagc tggttgcgca cgtcggcgac gcccgaggtg ttggcggcga
     1501 tcagctcggc gagttccttc tcggtttccg agtcgacctc gccctcgagc atgacgatct
     1561 tgtcgtcggt cgagacgttg atggccaggc ccgaggtgtt gctgctggcc agcaggcgcg
     1621 tcttgacgcg cgtggacagc gccgcatcac cggcggtcgc ctgcatgcgc tcgagcatgc
     1681 cgggctcgcc gcccgtcacg cgcagctcgt tcttcaccga cttgacgccg tccacggacc
     1741 gagcgagctc ggtagccatt tccttcctgg cgtcggtctc cacgtcgccc ttgagatgga
     1801 ccacgccgtg agtcacgtcg gtgctgatgg ccagcgcgct gagctcatcg ctctgcgcga
     1861 acttggtttc cagcgcgaga tgcatgcgga tgtcgttcgc cgtgtcgcgc gcctggtcgg
     1921 tcatcgtctg ctcggagcgc atctccttct ccttggtctt gtccttcttg gcatcggcgt
     1981 cataggcgcc ggcggcga
//
LOCUS       DAOMPG010000334         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_22172_length_1982_cov_3.980280, whole genome shotgun sequence.
ACCESSION   DAOMPG010000334 DAOMPG010000000
VERSION     DAOMPG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22172_length_1982_cov_3.980280"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLS34_12735"
     CDS             complement(<1..756)
                     /locus_tag="PLS34_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HRP88366.1"
                     /translation="MAEFDRLRRAQLEFAAHIRDPQHAPAPPGIEDRRMAIYRELFFN
                     NVRDLLGRSFPVLRALLGPTGWETLVRDWLVRHRARTPLFLELPREFLAYLQEEREPP
                     VGDPPFMTELAHYEWVELALSIDEREPELPGVDEGGDLLAGRPVLSPLAWSLAYAWPV
                     HRISPEFRPTEPPAEPTRLVVYRDRRDQVGFLEINLVTARLLELLAGEASPRPTGREC
                     LLRIAAELGHPAPDAVVAAGTALLADLRARDVVL"
     gene            complement(749..1636)
                     /locus_tag="PLS34_12740"
     CDS             complement(749..1636)
                     /locus_tag="PLS34_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRP88367.1"
                     /translation="MGKANQERTDMQDHYPVSGVGLGLRRSMMDELMADPPWGVDFME
                     VAPENWIGVGGSLGRKFCWFTDRFPFVTHGLSLSLGGPAPLDERFLEQARGFLDAHGI
                     RFYTEHLSYTGDEGHLYDLLPIPFTEEAVRHVAGRVRRAMEILERPIAVENVSYYAAP
                     GRELDEVRFLEAVLEEADCGLLLDVNNVYVNSINHGYDADEFIAALPAERIVYLHVAG
                     HYVEAEDLRVDTHGADVADPVWALLDRTYARFGPVPTLLERDFNIPPMATLNRELDII
                     RGLQRRYDSDTPAQDGAHG"
     gene            complement(1648..>1982)
                     /locus_tag="PLS34_12745"
     CDS             complement(1648..>1982)
                     /locus_tag="PLS34_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HRP88368.1"
                     /translation="QVADVIEQHPEYHALLGRDGDALDRDWTPEHGESNPFLHMGLHL
                     ALREQVATDRPAGIAALHRRLAARHGEHEAEHRMAECLAEALWRAQRNNSLPDEVSYL
                     EALHRIGP"
BASE COUNT          298 a          684 c          709 g          291 t
ORIGIN      
        1 gagcacgacg tcgcgcgccc gcaggtcggc cagcaacgcg gtgccggccg ccaccaccgc
       61 gtcgggcgcc gggtggccca gttcagcggc gatcctgagc aggcactcgc gcccggtcgg
      121 acgcggactc gcctcgccgg ccagcagctc cagcagccgc gccgtcacca ggttgatttc
      181 gagaaagccg acctggtcgc gacggtcccg gtagaccacc agccgggtcg gttccgccgg
      241 tggctcggtg ggccggaatt cgggcgagat gcggtgcacg ggccaggcgt aggccagcga
      301 ccatgccagt ggcgagagca cgggccggcc tgcgagcagg tcgccacctt cgtccacgcc
      361 gggcagctcc ggctcgcgct cgtcgatgga cagcgccagc tccacccact cgtaatgcgc
      421 cagctcggtc atgaacgggg gatcgcccac gggtggctcg cgttcctcct gcaggtaggc
      481 gagaaactcc cgcggcagct cgagaaacag cggcgtccgc gcgcggtggc gcaccagcca
      541 gtcgcgtacc agggtttccc agccggtcgg cccgagcagt gcgcgcagca ccgggaagct
      601 gcgcccgagc aggtcgcgga cattgttgaa gaacagttcg cggtagatcg ccatgcggcg
      661 atcctcaatg ccgggcgggg ccggcgcgtg ctgcgggtcg cggatatgcg cggcgaattc
      721 gagctgcgca cgccgcaggc gatcgaactc agccatgcgc gccatcctgc gccggcgtgt
      781 cgctgtcgta cctgcgctgc aagccgcgga tgatgtcgag ctcgcggttc agcgtggcca
      841 tcggcgggat gttgaagtcg cgctcgagca gcgtgggaac cgggccgaag cgcgcgtagg
      901 tgcggtcgag cagcgcccac accggatccg cgacgtccgc gccatgcgtg tccacccgca
      961 ggtcctcggc ctcgacgtag tggcccgcca cgtgcaggta cacgatccgc tcggcgggca
     1021 gggcggcgat gaactcgtcc gcatcgtagc cgtggttgat cgaattgacg tagacgttgt
     1081 tgacgtccag cagcaggccg cagtcggcct cctcgagcac ggcctcgaga aagcggactt
     1141 cgtccagttc ccggccgggc gcggcgtagt acgacacgtt ttcaaccgca atcggccgct
     1201 cgaggatctc catggcgcgg cggacgcggc cggccacgtg gcgcaccgcc tcctcggtga
     1261 agggaatcgg cagcaggtcg tagaggtgtc cctcatcgcc ggtgtaggag agatgctcgg
     1321 tgtagaagcg gatgccgtgc gcgtccagga agccgcgcgc ctgctcgagg aaacgctcgt
     1381 caagcggcgc cggcccgccg agcgacaaag acaggccgtg ggtcacgaaa ggaaagcggt
     1441 cggtgaacca gcagaacttg cggcccagcg agccgccgac gccgatccag ttctcgggcg
     1501 ccacctccat gaaatccacg ccccacggcg ggtccgccat gagctcgtcc atcatgctgc
     1561 gcctgaggcc gagcccgacc ccgctgaccg ggtagtggtc ctgcatgtcc gtccgctcct
     1621 gattcgcctt gcccatggcg cgccgcttca gggcccgatg cggtgcaggg cctccaggta
     1681 ggagacctcg tcgggcagcg aattgttacg ttgggcgcgc cacagcgcct ctgcgaggca
     1741 ctccgccatg cggtgctcgg cctcgtgctc gccgtgccgg gcggcgagcc ggcggtgcag
     1801 cgcggcgatc ccggccggcc ggtccgtggc gacctgctcg cgcagcgcga ggtgcaggcc
     1861 catgtgcagg aacgggttgc tctcgccgtg ctcgggcgtc cagtcgcggt ccagcgcatc
     1921 gccgtccctg ccgagcagtg cgtggtactc ggggtgctgc tcgatcacgt cggcgacctg
     1981 gt
//
LOCUS       DAOMPG010000335         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_22528_length_1957_cov_2.627234, whole genome shotgun sequence.
ACCESSION   DAOMPG010000335 DAOMPG010000000
VERSION     DAOMPG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22528_length_1957_cov_2.627234"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..602)
                     /locus_tag="PLS34_12750"
     CDS             complement(81..602)
                     /locus_tag="PLS34_12750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP88369.1"
                     /translation="MRKAFLRAPVDFTRVSSNFNPNRLHPVLKTRRPHQGVDYAARTG
                     TPIMAAGDGRVISAGVNGGYGKAVVLQHGGNVTTLYAHMSRIAPGMRAGRKVRQGEII
                     GYVGMTGLASGPHLHYEYRVNGVHKNPRTVELPKADPVPAARRAEFVAATLPLVAELE
                     GLGPTRLAAADPQ"
     gene            complement(557..1498)
                     /locus_tag="PLS34_12755"
     CDS             complement(557..1498)
                     /locus_tag="PLS34_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM-like peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HRP88370.1"
                     /translation="MASGIRHDYKEIARPPTVQRRPWSRRHVAVVVSLAVSAGLVALW
                     MDEPALGKQAPRATDPEPLTASQSLPLPTPAGAGLTPALPSADQAVAAMPVPPAALEP
                     EDTRQRTVLEVGPGDSLDRLFRRHELSVADLHRLLKVKEAAPHLKRIRPGDSFEILHD
                     GASVLGLTRRLDEARSLVVSTGEDGFAAEFLDHPVEVRRARVTGRITSSFYAAGLAAG
                     LADGTIMRLAGIYAWDIDFALDIRTDDWFAVVYEEIWQDGEKLRDGEIVATEFSNQGR
                     STAPSASPTRTAGRAITRPRATACARPSCARRSTSPA"
     gene            1610..>1957
                     /gene="tyrS"
                     /locus_tag="PLS34_12760"
     CDS             1610..>1957
                     /gene="tyrS"
                     /locus_tag="PLS34_12760"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRP88371.1"
                     /translation="MRPANEQLAELQRGTEDVLVEKELEERLASGRPLRIKAGFDPTA
                     PDLHLGHTVLINKMRQFQAFGHEVIFLIGDFTGMIGDPTGKSATRPPLTRAEIEENAR
                     TYEAQIFKILDAGK"
BASE COUNT          298 a          651 c          716 g          292 t
ORIGIN      
        1 gtcgatgccg tccatcgagg tcccggagag cagcccgata tagaggccgc tgagcactgc
       61 cgtggcgccg cgcgtgcggc ttactggggg tcggctgcgg ccagccgcgt cggcccgaga
      121 ccctcgagtt cggcgaccag cggcagggtg gcggcgacga actcggcacg ccgcgcggcc
      181 gggaccggat cggccttggg cagctcgacg gtgcgtggat tcttgtgcac gccgttgacg
      241 cggtactcgt agtgcaggtg cggcccgctg gcgagcccgg tcatgccgac atagccgatg
      301 atctcgccct ggcggacctt gcggccggcg cgcatgccgg gcgcaatgcg cgacatgtgg
      361 gcgtacagcg tggtcacgtt gccgccgtgc tgcagcacga ccgccttgcc gtagccgccg
      421 ttcacgccgg cggagatcac ccggccgtcg ccggccgcca tgatgggcgt gccggtacgg
      481 gccgcgtagt ccacgccctg gtgcgggcgg cgggtcttca gcaccgggtg caggcggttg
      541 gggttgaagt tcgagctcac gcgggtgaag tcgaccggcg cgcgcaggaa ggccttgcgc
      601 atgctgtcgc cctcgggcgt gtaatagcgc ggccggccgt cctcgtcggc gaagcggacg
      661 gcgcggtaga ccggccctgg ttgctgaact cggtggcgac gatctcgccg tcgcgcagct
      721 tctcgccgtc ctgccagatt tcctcgtaga ccacggcgaa ccagtcgtcg gtccggatgt
      781 cgagggcgaa gtcgatgtcc caggcataaa tgcctgcgag gcgcatgatg gtgccgtcgg
      841 ccaggcccgc ggccaggccc gcggcgtaga aggaactggt gatccggccg gtgacgcggg
      901 cccgccgcac ttccacgggg tgatcaagga actcggccgc gaagccgtct tcgccggtgc
      961 tgaccaccag gctgcgcgcc tcgtccagcc gccgggtcag gcccagcacg ctggcaccgt
     1021 catggaggat ctcgaagctg tcgccgggac ggatgcgctt caggtgcggc gcggcttcct
     1081 tgaccttgag caggcggtgg aggtcggcca ccgacagctc gtggcggcgg aacaaccggt
     1141 cgaggctgtc gccggggccg acttccagca cggtgcgctg gcgggtgtct tcgggctcga
     1201 gcgcggccgg cgggacgggc attgccgcca cggcctggtc ggccgacggc agggcgggcg
     1261 tcaagccggc gccggcaggc gtcggcagcg gcagcgactg gcttgccgtg aggggttcgg
     1321 ggtcggtagc gcgcggcgcc tgcttgccca gggccggctc gtccatccac agggcgacca
     1381 ggccggcgga cactgcgagg ctgaccacga cggcgacgtg acggcgggac cacggccggc
     1441 gctgcaccgt tggcgggcgc gctatttcct tgtaatcgtg ccggattccg cttgccactc
     1501 gacccaccgc gccgcaggct gcaaaattga ggctacccta gcggccccgg gcgttccggt
     1561 caatgcgcca attctcataa taaaaacagc aagtcatcct ggagtgttaa tgagacctgc
     1621 gaacgaacag ctcgccgagc tgcagcgcgg cacggaagac gtgctggtgg agaaggagct
     1681 cgaggagagg ctggcgagcg gccggccgct gcgcatcaag gccggcttcg atccgaccgc
     1741 ccccgacctg catctcggcc acaccgtgct gatcaacaag atgcgccagt tccaggcctt
     1801 cggccacgag gtgatcttcc tcatcggcga cttcaccggg atgatcggcg atcccaccgg
     1861 gaagagcgcc acgcgcccac cgctgacccg cgcggaaatc gaggagaacg cgcgcaccta
     1921 cgaggcgcag atattcaaga tcctggacgc cggcaag
//
LOCUS       DAOMPG010000336         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_22713_length_1942_cov_4.251192, whole genome shotgun sequence.
ACCESSION   DAOMPG010000336 DAOMPG010000000
VERSION     DAOMPG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22713_length_1942_cov_4.251192"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLS34_12765"
     CDS             complement(<1..757)
                     /locus_tag="PLS34_12765"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRP88372.1"
                     /translation="MSLCRRLTPMLAMASCLCALLAFEPSMAEQAAQLERMTVTGNRA
                     QALAQAQFAKMRDEQMQDALSGSGGGGGSGGQAPAPPANNDRAVDERDCEETDNPVVL
                     SSGNKVLDEHDFATAVGDFVVGRTYSRNGDFFSGFGPNWSWSLGYQLKFQTNPAWTPV
                     CQPGTYEPGVPCPLVPGKFLKVTAVRPDGHRYDYTWNAAAQRYEDSRPVSTSWIVEEL
                     WSGASPLSALTLWREDGGRERYKENGQVLQVRDL"
     gene            1002..1931
                     /locus_tag="PLS34_12770"
     CDS             1002..1931
                     /locus_tag="PLS34_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRP88373.1"
                     /translation="MSWHACPQAATAPAPSPADPVEIEIVPRSRDLGGFEVRRALPSG
                     RRRMVGPFVFWDQMGPSMLRPGSGLDVRPHPHIGLATLTYLFRGEILHRDSLGTVQPI
                     CPGAVNWMTAGRGIVHSERTPPELRPTGPELFGIQAWIALPVQHEETAPDFSHHAAAD
                     LPLVEDTGLRLRVIAGEAFGGRSPVPTFSETLYVDAALAAGAVLPVEPTHEERALYLV
                     EGAVEIAGRRHEPGRLLVLQPGESLGVKALEDARLLLLGGEPMDGPRFIWWNFVSSRK
                     ERIEQAKADWREGRFAAVPGDDEFIPLPEEPGG"
BASE COUNT          298 a          676 c          657 g          311 t
ORIGIN      
        1 ggagatcgcg gacctgcagc acctggccgt tctccttgta gcgctcccgc ccgccgtcct
       61 cgcgccacag cgtgagtgcg ctcagcggcg aggcgccgct ccacaactcc tcaacgatcc
      121 agctggtcga gaccggccgt gaatcctcgt agcgctgggc cgcggcattc caggtgtagt
      181 cgtagcgatg cccgtccggt cgcaccgccg tgaccttgag aaacttgccg ggcaccagcg
      241 ggcacggtac cccgggctca tacgtgccgg gctggcacac cggcgtccac gccgggttgg
      301 tctggaactt caactgatag ccaagcgacc agctccagtt cggtccgaaa ccgctgaaga
      361 agtcgccgtt cctggaatac gtccgcccga ccacgaaatc tccgactgcc gtcgcgaagt
      421 cgtgctcgtc cagtaccttg ttaccggagg acaagaccac cgggttgtcg gtttcctcgc
      481 agtcccgctc gtccacggcg cgatcattgt tcgccggagg cgcaggcgcc tgcccgcccg
      541 agcccccgcc accgcccgac ccgctgagcg catcctgcat ttgctcgtcg cgcatttttg
      601 cgaactgggc ctgcgccaat gcctgcgccc ggttgccggt aacggtcatc ctttcgagct
      661 gcgcggcctg ctcggccatc gacggctcga aagcgagcag ggcacatagg caagacgcca
      721 tggcgagcat cggcgtcagt cggcggcaaa gcgacatcga tgcttcggct gtacggtacg
      781 ggatggacat ggctacctcg atgtgagcgc gatatatggg tacattcgcc caaatatgcc
      841 ccccccttga ggtgttttgc aagactgtcg aactatgtgt acgccatggt atgcagacag
      901 gcccgcgaga cgagccccca acgccagatg gcagccgctg ctagactccg ggcgaaccgc
      961 acgcaagcgc ggtttcacta tccttcgcca aggagacacg catgagctgg cacgcctgtc
     1021 cgcaagccgc aacggccccg gcgccctcgc cggccgaccc ggtcgagatc gagatcgtgc
     1081 cgcgcagccg cgacctcggc ggcttcgagg tgcggcgcgc gctgccctcg gggcgccgcc
     1141 gcatggtcgg gcccttcgtg ttctgggacc agatgggacc gagcatgctg cgccccggca
     1201 gtggtctcga cgtgcggccg catccgcaca tcggcctcgc caccctcacc tacctgttcc
     1261 gcggcgagat cctgcatcgc gacagcctcg gcacggtgca gccgatctgc ccgggcgcgg
     1321 tgaactggat gaccgccggg cgcggcatcg tgcactccga gcgcacgccg ccggaactgc
     1381 gtccgacggg gcctgagctg ttcggcatcc aggcgtggat cgcactgccc gtgcagcacg
     1441 aggagaccgc gccggacttc tcgcaccatg cggcggccga cctgccgctg gtcgaggaca
     1501 ccggcctgcg gctgcgcgtg atcgcgggcg aggccttcgg cggccgctcg ccggtgccga
     1561 cattcagcga aacgctgtac gtggatgcgg cgctggccgc cggcgccgtg ctgccggtcg
     1621 agccgacgca cgaggaacgg gcgctctacc ttgtcgaggg cgcggtggag atcgcggggc
     1681 ggcggcacga gccgggccgg ctgctggtgc tgcagccggg tgaatcgctc ggcgtgaagg
     1741 cgctggagga tgcacgcctg ctgctgctcg gcggcgagcc gatggacggc ccgcgcttca
     1801 tctggtggaa cttcgtgtcc tcgcgcaagg agcgcatcga gcaggccaag gcggactggc
     1861 gcgagggccg ctttgctgca gtgccgggcg acgacgagtt catcccgctg ccggaggaac
     1921 cgggcggctg agcggcgttg ct
//
LOCUS       DAOMPG010000337         1835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_24314_length_1835_cov_3.202247, whole genome shotgun sequence.
ACCESSION   DAOMPG010000337 DAOMPG010000000
VERSION     DAOMPG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24314_length_1835_cov_3.202247"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..716
                     /locus_tag="PLS34_12775"
     CDS             18..716
                     /locus_tag="PLS34_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88374.1"
                     /translation="MNLHPASLNLHLLAGMLALLSYWTAALARKGSRPHVFAGRVYLL
                     AMVGIVVSGIPLVARFALEGRPVMASFFGYLLLLVCWTCWCAWRAVRDRRHPQRYAGP
                     VYLVLSALVLAAGLAIVALGAARGAAILVVFGLIGVLAGGGALRVRQRLQDNPRWWLK
                     EHYGAMIGNGVATHVAFMSIGLRALLPEMDGATLTYLAWFGPLAAAVAAGWWLDRRYL
                     RRPVFSGREGPCLE"
     gene            798..1448
                     /locus_tag="PLS34_12780"
     CDS             798..1448
                     /locus_tag="PLS34_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRP88375.1"
                     /translation="MKLPIQDRIAAGRALAALLADRYRNEDVIVLALPRGGVPVAAEI
                     ARALAAPLDVLNIRKLGVPFQPELAMGAIASGGIRVLNPEVIRLAGVDRDTIEEVVTM
                     ETRELERRERLYRGDRARPPLAHRCVIIVDDGVATGATMDAAVEAARQLGPARVVVAI
                     PLAPPDVIARLEAHADEVVCLAAPAPFFSIGQWYEDFRQVGDDEVRALLGREPAAA"
     gene            1467..>1835
                     /locus_tag="PLS34_12785"
     CDS             1467..>1835
                     /locus_tag="PLS34_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRP88376.1"
                     /translation="MARILALDFETASHAPDSACALGLALVEDGRLVAEDAFLIRPPE
                     PEFVFTWVHGLRWEDVADQPPFDSAWLRLAPWLEGVDWLAAHNAPFDRGVLNACCRTY
                     RLAAVDLPFLCTVQVARQVWG"
BASE COUNT          212 a          632 c          678 g          313 t
ORIGIN      
        1 tgagagggag gtcggacatg aatctgcacc ctgcatcgct gaatcttcac ctgcttgccg
       61 gcatgctcgc gctgttgagc tactggacgg ccgcgctggc gcgcaagggc agccggccac
      121 atgtattcgc tggtcgggtc tacctgctgg cgatggtcgg catcgtggtt tcgggcattc
      181 cgctggtggc gcgcttcgca ctcgagggcc gccccgtgat ggcctcgttt ttcggctacc
      241 tgctgctgct ggtgtgctgg acctgctggt gcgcctggcg cgcggtgcgg gaccgccgac
      301 acccgcagcg ctatgccgga ccggtgtacc tggtgttgag cgccctggtg ctggccgccg
      361 ggctggcgat cgtcgccctc ggggcggccc gcggcgccgc catcctggtc gtgttcggcc
      421 tgatcggcgt gctggccggc ggcggcgccc tgcgggtccg gcagcgcctg caggacaacc
      481 cccgctggtg gctcaaggag cactacggcg ccatgatcgg caacggcgtg gccacgcacg
      541 tcgccttcat gtccatcggg ctgcgcgccc tgctgccgga gatggacggc gcgaccctga
      601 cttacctggc gtggttcggg ccgctggctg cggccgtggc ggcgggctgg tggctggatc
      661 gccgctacct ccgccggcct gtcttttccg gccgcgaggg tccatgcttg gagtaggcag
      721 gaacgggcgg aatcggcccg cattcgactc cgtccgtcgt cgccgaacac cggcacggaa
      781 tgcaacggag gtgtcccatg aagctgccca tccaggaccg cattgcggcg gggcgcgcgc
      841 tggccgcgct gctcgcggac aggtaccgga acgaggacgt gatcgtgctg gccctgccgc
      901 gcgggggcgt gccggtggct gcggagattg cccgcgcgct ggccgcgccg ctcgacgtgc
      961 tgaacatccg caagctcggc gtgccctttc agcccgagct tgccatgggc gcgatcgcca
     1021 gcggcgggat ccgggtgctg aaccccgaag tcattcgtct cgcaggcgtc gatcgcgaca
     1081 ccatcgagga ggtcgtcacc atggaaaccc gcgagctcga gcgccgcgag cgtctctacc
     1141 gcggggaccg cgcgcggccg ccgctggcgc accgctgcgt gatcatcgtg gacgacggcg
     1201 tggcgacggg cgcgaccatg gacgcggccg ttgaagccgc caggcagctc ggcccggcgc
     1261 gcgtggtggt tgcgattccg cttgcgccgc cggatgtcat cgcacgactg gaggcgcacg
     1321 ccgacgaggt cgtctgcctc gccgcgccgg cgcccttctt ctcgatcggg cagtggtacg
     1381 aggatttccg ccaggtcggt gacgacgagg tgcgggcgct gctcggccgc gagccggcgg
     1441 ccgcctagcg cgcgccggcc gcggtcatgg cacgaatcct ggcgctggat ttcgagacgg
     1501 ccagccatgc gcccgacagc gcctgcgcgc tcggcctggc tctggtcgag gacggccggc
     1561 tggtggccga agacgctttc ctgatccggc cgccggagcc ggagttcgtg ttcacctggg
     1621 tgcacggctt gcgctgggag gacgtcgccg atcagccgcc gttcgactcc gcctggttgc
     1681 gcctcgcgcc ctggctggag ggcgtcgact ggctggctgc gcacaatgcg ccctttgacc
     1741 gcggcgtgct gaacgcctgc tgccggacct atcgcctggc cgccgtggac ctgccgtttc
     1801 tctgcaccgt gcaggtcgcg cgccaggtct ggggc
//
LOCUS       DAOMPG010000338         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_24406_length_1829_cov_3.412063, whole genome shotgun sequence.
ACCESSION   DAOMPG010000338 DAOMPG010000000
VERSION     DAOMPG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24406_length_1829_cov_3.412063"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /gene="msbA"
                     /locus_tag="PLS34_12790"
     CDS             <1..1448
                     /gene="msbA"
                     /locus_tag="PLS34_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRP88377.1"
                     /translation="EVFAKFLVLPTRFYDTSSSGALLSRLTFNIEQVAQAASQVLTTL
                     FKDVLTILGLVAYMFWINARLSLFVLVTAPLIGLVISYLSKKFRRYSTRIQNSMGDVT
                     QAAEQVLEAHKVVKVFNGEAQEVEGFERVNEQNRRLNMKLAAARAASQPVVQLVAAIG
                     LAGVVFVATREDLAGGLSVGDFVSFLGAMLLIMAPLKHLTNINGPLQQGIAAGQSIFE
                     LLDAPGEDEGGERRLQRARGEVEFRAVNFVYQQEKGQVLRDVSLRVAPGERLAIVGRS
                     GSGKSTLVSLIPRFYDPTGGTVLVDGHDVREYRRADLRRQVSLVTQEVKLFDDTIRSN
                     IAYGAMRAASPAEIEAAARAAHVLEFTDRFPDGLETEVGDRGVTLSGGQRQRVAIARA
                     LLKNAPILILDEATSALDSESERHIQEALEALMQNRTTLVIAHRLSTIEHADRIVVMD
                     HGRIVEVGSHGELMALDGAYAALHRMQFQVS"
     gene            1445..>1829
                     /locus_tag="PLS34_12795"
     CDS             1445..>1829
                     /locus_tag="PLS34_12795"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRP88378.1"
                     /translation="MSAAPQRLSNAWYGTAPPPWWARSLEPLYRSLAAARRGAYRRGW
                     LRSGHPGCPVVIVGNITAGGAGKTPLVAHVVQLLAARGCAPAIVSRGYGGAEPRRPHR
                     VSSDDPVAITGDEPRLLAAMTGRPVW"
BASE COUNT          275 a          624 c          653 g          277 t
ORIGIN      
        1 aggaagtgtt cgccaagttc ctggtgctgc cgacgcgctt ctacgacacc agctcgtccg
       61 gcgcgctgct gtcgaggttg actttcaaca tcgagcaggt cgcccaggcg gccagccagg
      121 tgctcaccac gctgttcaag gacgtgctca ccatcctcgg cctggtcgcc tacatgttct
      181 ggatcaacgc gcggctgtcc ttgttcgtgc tggtgaccgc accgctgatc ggactggtca
      241 tcagctacct gtcgaagaaa ttccgtcgct acagcacgcg catccagaat tccatgggcg
      301 acgtcacgca ggccgccgag caggtgctcg aggcccacaa ggtggtcaag gtgttcaacg
      361 gcgaggccca ggaagtggag ggcttcgagc gggtcaacga gcagaaccgc cgcctcaaca
      421 tgaagctggc cgccgcgcgg gccgcaagcc agccggtggt gcagctggtt gccgcgatcg
      481 gcctggccgg cgtggtgttc gtcgccacgc gcgaggacct cgccggcgga ctctcggtcg
      541 gcgatttcgt gtccttcctc ggcgccatgc tgctcatcat ggcgccgctc aagcacctca
      601 ccaacatcaa cggcccgctg caacagggca ttgcggcagg ccagagcatc ttcgagctgc
      661 tggacgcgcc cggcgaggac gagggcggcg agcggcgcct gcagcgcgcc cgcggcgaag
      721 tggagttccg cgcggtgaac ttcgtctacc agcaggagaa gggccaggtc ttgagggacg
      781 tcagcctgcg cgtcgcgccg ggcgagcggc tcgcgatcgt ggggcgctcc ggcagcggca
      841 agagcacgct cgtcagcctg atcccgcgct tctacgatcc caccgggggg acggtgctgg
      901 tggacgggca cgacgtgcgc gagtaccggc gcgccgacct gcgccgccag gtcagcctgg
      961 tgacccagga ggtcaagctg ttcgacgaca ccattcgcag caacatcgcc tacggcgcga
     1021 tgcgcgctgc gagcccggcg gagatcgagg cggcggcgcg cgcggcccac gtgctggagt
     1081 tcaccgaccg gtttccggac gggctcgaga ccgaggtcgg cgatcgcggc gtgacgctgt
     1141 cgggcggcca gcgccagcgg gtcgcgatcg cgcgggcgct gctcaagaac gcccccatcc
     1201 tgatcctgga cgaggccact tcggcgctcg actcggagtc ggagcgccac atccaggagg
     1261 cgctggaggc gctgatgcag aaccgcacca cgctggtgat cgcgcaccgt ctgtcgacca
     1321 tcgagcatgc ggaccggatc gtggtgatgg accacggccg cattgtcgag gtcggctcgc
     1381 acggcgagct gatggcgttg gacggcgcct acgccgcgct gcacaggatg cagttccagg
     1441 tctcgtgagc gcggccccgc aacgcctgtc gaacgcctgg tacggcacgg cgccgccgcc
     1501 atggtgggcg cgctcgctcg agcctctcta tcgctcgctg gccgcggcgc gacgcggcgc
     1561 ctatcggcgc ggctggctgc gctcaggaca tccgggctgc ccggtggtga tcgtcggcaa
     1621 catcaccgcc ggcggcgccg gcaagacgcc gctggtggcc catgtcgtgc agctgctcgc
     1681 ggcccgtggg tgcgccccgg cgatcgtcag ccgcgggtac ggcggggcgg aaccgcgccg
     1741 gccgcatcgc gtgagcagcg acgatcccgt tgcgatcacc ggcgatgagc cgcggctcct
     1801 ggcggccatg accggtcgcc cggtgtgga
//
LOCUS       DAOMPG010000339         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_25170_length_1783_cov_4.635417, whole genome shotgun sequence.
ACCESSION   DAOMPG010000339 DAOMPG010000000
VERSION     DAOMPG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25170_length_1783_cov_4.635417"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLS34_12800"
     CDS             complement(<1..268)
                     /locus_tag="PLS34_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HRP88379.1"
                     /translation="MTRKFLALGAGLVLSVSAFGQALQVDPALPDYKPASGVSGNLKS
                     IGSDTLNNLMALWAEGFRSAYPNVQIEIEGKGSGTAPPALIGGNA"
     gene            524..1648
                     /gene="dapE"
                     /locus_tag="PLS34_12805"
     CDS             524..1648
                     /gene="dapE"
                     /locus_tag="PLS34_12805"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRP88380.1"
                     /translation="MSQVLELAKALVARPSITPDDADCQELMIRRLEPLGFRIERMRV
                     GEVDNFWARLGSEGPLLCFAGHTDVVPTGPVETWRSQPFVPTERDGQLWGRGTADMKA
                     SLAAMVVACEEYLAGNPAPAGSIAFLITSDEEGPAKDGTRAVIERLEARGEKIDWCLV
                     GEPSSRERLGDMLRIGRRGSLSGDLVVHGVQGHVAYPESAVNPLHKLAPVLAELVTLE
                     WDAGNESFPPTSFQLTNLHSGTGFRNVTPGSAELKFNIRYSTEQTFEGLQARIHEVLD
                     RHGLDYTIAWRDAGRPFLTRGGPFLEAVRAVVREIAGVDPELSTGGGTSDGRFIAPTG
                     ADVVELGPLNASIHKIDEHVRIADLEPLKDLYLGILRRLL"
BASE COUNT          288 a          602 c          620 g          273 t
ORIGIN      
        1 gggcgttgcc gccgatcagc gccggcggcg cggtgcccga gcccttgccc tcgatttcga
       61 tctggacgtt cgggtaggcc gagcggaagc cctcggccca cagggccatc aggttgttca
      121 gcgtgtcgga accgatggac ttcaggttgc cggaaacgcc ggaggcgggc ttgtagtctg
      181 gcagggccgg atccacctgc agcgcctggc cgaaggccga gacggagagg accagaccgg
      241 cgccaagcgc gagaaacttg cgggtcattc gtagactcct tggatagcgt ttggagatgt
      301 cgtcaaaaaa ccggccgtgc cgggaacggg gcatacccta agcgccgcct gttacatgaa
      361 gatgacaagc cggcgccgat gtcacaattc gccgcgaccg gccataatcg gggccgccgt
      421 cgctgccggc ggggcgcccg gccgaccgtc gcccggccgt ccggcacgtt gtttttcaag
      481 ggcttccggc cgccacactt ggcctctaaa caggattaag tttttgtcac aagtacttga
      541 gctcgctaag gcattggtgg cccgaccctc catcaccccg gacgacgccg actgccagga
      601 actgatgatc cggcggctcg agccgctggg tttccgcatc gagcgcatgc gggtcggcga
      661 ggtggataat ttctgggcgc ggctggggag cgagggcccg ctgctgtgtt tcgcgggcca
      721 caccgacgtg gtgccgaccg gcccggtgga gacctggcgc tcgcagccct tcgtgcccac
      781 cgagcgcgac ggccagctgt ggggccgcgg cacggccgac atgaaggcca gcctggcggc
      841 catggtggtg gcctgcgagg aatacctggc gggcaatccc gcgccggccg gctcgatcgc
      901 gttcctgatc accagcgacg aagagggccc ggcgaaggac ggcacccggg ccgtgatcga
      961 gcggctcgag gcgcgcggcg agaaaatcga ctggtgcctg gtcggcgaac cgtccagccg
     1021 cgagcggctc ggcgacatgc tgcgcatcgg gcggcgcggc tcgttgtccg gcgacctggt
     1081 ggtgcacggc gtgcagggcc atgtcgcata tcccgagtca gcggtgaacc cgctgcacaa
     1141 gctggcaccc gtgcttgccg aactggtcac gctggaatgg gacgccggca acgagtcctt
     1201 cccgccgacc agcttccagc tcaccaacct gcactccggc accggctttc gcaacgtcac
     1261 gccgggctcg gccgaactga agttcaacat ccgctactcg accgagcaga ccttcgaggg
     1321 attgcaggcg cgcatccacg aggtgctgga ccgccacggg ctggactaca cgatcgcctg
     1381 gcgcgacgcc ggccggccct tcctgacgcg cggcggcccg ttcctcgagg cggtgcgcgc
     1441 ggtggtgcgc gagatcgccg gcgtggatcc cgagctgtcc accggcggcg gcacctcgga
     1501 cggccgcttc atcgcaccga ccggtgccga tgtcgtggag ctcgggccgc tcaacgccag
     1561 catccacaag atcgacgagc acgtgcgcat cgccgacctg gagccgctca aggacctcta
     1621 cctcggcatc ctgcgacggc tcctctgaac cggggtcaga caccggtttc cgaaactggg
     1681 gtcagacacc ggtttcggtc gaccgcgggc gcgcgggggc cttcggttcc agtcgaccgc
     1741 gggcgcgcgg cgccataggc cggcctgcgc tcggacaagg aaa
//
LOCUS       DAOMPG010000340         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_25276_length_1777_cov_5.027294, whole genome shotgun sequence.
ACCESSION   DAOMPG010000340 DAOMPG010000000
VERSION     DAOMPG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25276_length_1777_cov_5.027294"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLS34_12810"
     CDS             complement(<1..268)
                     /locus_tag="PLS34_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88381.1"
                     /translation="MPCSPAPPRLLGRDDGPRVLAVGRRPPPRRHARLEWLQAELEDL
                     EDALSGRSFDQAFCCLGTTMKRAGSRDAFRAVDLDGVTAFARAAR"
     gene            627..>1777
                     /locus_tag="PLS34_12815"
     CDS             627..>1777
                     /locus_tag="PLS34_12815"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HRP88382.1"
                     /translation="MHPSVIWFGKMRDAQMRDFLSGASGPGRPLGAPPPPANNDRSAD
                     PNNPCADESDNPVVLTSGNKVLDEHDFATAPGDFVVGRTYSRNGGFFVGFGPNWSWSL
                     GYQLKFEPNPAWTPVCQPGTAEPGMPCPLVPGKFLKVTAVRPDGHRYDYTWNATSQRY
                     EDSRPVSTSWIVEELWSGASPLSALTLWREDGGRERYKENGQVLQVRDHREVGYTFNY
                     INLGNTLNTITHTSGRSITLAWSGGRISSITAPNGKVWSYGYNNGRLVNVTAPDGLGV
                     KTYHYEIGAQPEALTGYSIGGVRRTEYGYNSDGTVQYSGLAGGIERDTFVYGTNYTDV
                     TNARGHTTRYNFTTLNGIKRLTGVDRAQSTACPAAAVVIDYDSRGYVERR"
BASE COUNT          334 a          581 c          594 g          268 t
ORIGIN      
        1 cacgcgcggc gcgggcgaaa gcggtgacgc cgtcgaggtc cacggcgcgg aacgcatccc
       61 ggctgccggc gcgtttcatg gtggtgccca ggcagcagaa ggcctggtcg aagctgcggc
      121 ctgacaacgc gtcctcgagg tcctccagct cggcctgcag ccactcgaga cgcgcatgcc
      181 gccgcggcgg cggacgccgg ccgacggcca gcacgcgcgg gccgtcgtcg cgccccagga
      241 gccgcggtgg cgccggcgag cagggcaatg ggcggggagt tcatgggcgg cagggtgtgg
      301 tggtccgctc cttgatgtca atcacgcggt ggctgagcaa ggaaggcagc ccacctttga
      361 ggccgacttc tggatgaatg tgactgtcgg tcttgcaaaa tacctctagg ggggggcata
      421 tttgggcata aaagcccata tagcgacccc aaaccgaggt ggtcatgtcc aacccatacc
      481 atacgccgga agcatcgatg tcgttctgcc gccgactggc gccgatgctc ggcatggcgt
      541 cgtgcctctg tgtcctgctc gctttcgagc cgttgatggc tgaagatgcc gccgatttac
      601 cacgtgtaaa cgtcaccgga gagaggttgc atccatcggt aatctggttc gggaaaatgc
      661 gcgatgcgca gatgcgggac tttctcagcg gagcgagcgg cccaggacgc ccactaggcg
      721 caccgccccc accggccaac aacgatcgca gcgccgaccc gaacaaccct tgcgcggatg
      781 aatccgacaa cccggtagtc ctgacctcgg gcaacaaggt gctggacgag cacgacttcg
      841 cgaccgcgcc cggggatttc gtggtcgggc ggacgtattc gcgcaacggc ggcttcttcg
      901 tcggctttgg cccgaactgg agctggtcgc tggggtacca gctgaagttc gagcccaacc
      961 cggcgtggac cccggtgtgc cagccgggga cggcggagcc cggcatgcca tgcccgctgg
     1021 taccggggaa gtttctcaag gtcaccgccg tacgaccgga cgggcatcgt tacgactaca
     1081 cctggaatgc cacctcgcag cgctacgaag attcacgacc ggtctcgacc agctggattg
     1141 tcgaggagtt gtggagcggc gcctcgccac tgagcgcact cacgctgtgg cgtgaggacg
     1201 gcgggcggga gcgctacaag gagaacggcc aggtgctgca ggtccgcgat caccgggaag
     1261 tgggctacac cttcaactac atcaacctcg gcaacaccct gaacaccatc acccacacct
     1321 ccggccggag catcacgctg gcctggagcg gcgggcggat cagctcgatc acggccccca
     1381 atggcaaggt ctggagctac ggctacaaca acggccggct ggtgaacgtg accgccccgg
     1441 acggactcgg ggtgaagacc taccactacg agatcggcgc gcagccggag gccctgacgg
     1501 gatactccat cggcggcgtg cggcgcaccg agtatggcta caactccgac gggacggtgc
     1561 agtactccgg cctggccggc ggcatcgagc gcgacacctt cgtctacggt acgaactaca
     1621 cggacgtcac caatgcccgg ggccacacga cccgctacaa cttcacgacc ctgaacggaa
     1681 tcaagcgcct cacgggcgtg gaccgggcgc agagcaccgc ctgcccggcg gccgccgtgg
     1741 tcatcgatta cgacagccgt ggctacgtgg aacggcg
//
LOCUS       DAOMPG010000341         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_25279_length_1777_cov_3.148664, whole genome shotgun sequence.
ACCESSION   DAOMPG010000341 DAOMPG010000000
VERSION     DAOMPG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25279_length_1777_cov_3.148664"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLS34_12820"
     CDS             <1..286
                     /locus_tag="PLS34_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP88383.1"
                     /translation="DTEAVLRNIFDALTIPTAEGQVQPHLATAWRVVDPLTYEFDLRS
                     GVTFSNGEVLDADDVVFTFERILTEGAIDGATSPRAGVRRVARRARCAVP"
     gene            complement(420..>1777)
                     /gene="cls"
                     /locus_tag="PLS34_12825"
     CDS             complement(420..>1777)
                     /gene="cls"
                     /locus_tag="PLS34_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRP88384.1"
                     /translation="GTTLAWLIVLFVLPVFGPALYLFIGENRLGQKRAARWRVIHDLY
                     REWQGGLKARAAVDWGQLHPRAESLASHGESITGFVALQGHDLQLLPDWDSFVDALVA
                     DIEAARSTCHLEFYIWDSAGRVGEVYTALARAAARGVICRVLVDDVGSGRFLKSREAA
                     GLRKVGVKVVSSLPVGLFRMLLVRMDLRNHRKIVVIDGEIGYTGSMNLVDPAYFKTSA
                     GVGRWVDAMARLRGPAVEVLNGAFLEDWELETDEGMGILETADIRSVPEEGDAVVQVV
                     PSGPGLNRASIHQLLLSLLYSAERELILTTPYFVPDESIMTALESASRRGVAVTLNIP
                     ERLDSVLARFAGAACFEELLAAGVRIARYEPDLLHTKAITVDGRIAVMGSVNIDMRSL
                     WLNFEISLFIYDQACAARVRALQQDYLRDSVELHPRAWRERPLAQRAAEGVLRLLGPM
                     L"
BASE COUNT          293 a          632 c          590 g          262 t
ORIGIN      
        1 cgacaccgag gcggtgctcc gcaacatctt cgacgccctc accatcccca ccgccgaagg
       61 tcaggtgcaa ccgcatctgg caacggcttg gcgagtcgtc gacccactga cctacgagtt
      121 cgacctgcgc tccggcgtga ccttcagcaa cggcgaggtc ttggacgcgg acgacgttgt
      181 cttcaccttc gagcgcatcc tcaccgaggg cgccatcgac ggcgccacca gcccgcgcgc
      241 gggcgtccgc cgggtggcgc gtcgcgctcg atgcgcagtt ccttgagaag ttcggctttg
      301 tccatgagga tccggattct agcagcggcc atggtgcggc atgttgcaaa tcgcgggaag
      361 tgccgttggt catgcggccg ggctgacaga tgtcacccgg cagcgtctcg agcacccggc
      421 tagagcatcg gtcccagcag gcgcagcacg ccttcggcgg cccgctgggc caggggccgt
      481 tcgcgccagg cgcgggggtg gagctcgacc gagtcgcgca ggtagtcctg ctggagcgcg
      541 cgcacccgcg ccgcacaggc ctggtcgtag atgaacagcg agatctcgaa attcagccac
      601 aggctgcgca tgtcgatgtt caccgagccc atgaccgcaa tccggccgtc cacggtgatc
      661 gccttggtgt gcagcaggtc gggctcgtaa cgcgcgatgc gcacgccggc cgcgagcaac
      721 tcctcgaagc aggcggcgcc ggcgaagcgc gccagcaccg agtcgagccg ctccgggatg
      781 ttcagcgtca ccgcgacgcc acgccgcgag gccgactcca gcgcagtcat gatcgattcg
      841 tccggcacga aatacggcgt ggtcaggatc agctcgcgct cggccgaata cagcaacgac
      901 agcagcagct ggtggatcga ggcgcggttg agcccggggc ccgacggcac cacctgcacc
      961 accgcgtcgc cctcttccgg gacactgcgg atgtccgccg tctccaggat gcccatgccc
     1021 tcgtcggtct ccagctccca gtcctcgagg aaggcgccgt tgagcacctc gaccgccggc
     1081 ccgcgcagcc gcgccatcgc gtccacccag cggccgacgc cggcggaggt cttgaagtag
     1141 gccgggtcga ccaggttcat gctgccggtg taaccgatct cgccgtcgat cacgacgatc
     1201 ttgcggtggt tgcgcaggtc catgcgcacc agcagcatgc ggaacagccc gaccggcagc
     1261 gagctgacga ccttgacgcc gaccttgcgc agtcccgcgg cctcgcggct cttgagaaac
     1321 ctgccggagc cgacgtcgtc caccagcacc cggcagatga cgccgcgcgc ggcggcgcgc
     1381 gccagtgccg tgtagacctc ccccacgcgt ccggcggagt cccagatgta gaactccagg
     1441 tggcaggtgc tccgggccgc ctcgatgtcg gccaccagcg catcgacgaa gctgtcccag
     1501 tcgggcaaca gctggaggtc gtggccctgc agcgcgacga agccggtgat ggattcgccg
     1561 tggctggcca gcgattcggc gcgcgggtgc agctgtcccc agtccacggc cgcgcgggct
     1621 ttcaatccgc cctgccactc ccggtacagg tcgtgaatga cgcgccagcg ggcggcgcgt
     1681 ttctggccga gcctattctc gccgatgaac aggtacagcg cgggcccgaa caccggcagc
     1741 acgaacagca cgatcagcca ggccagcgtg gtgccga
//
LOCUS       DAOMPG010000342         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_26528_length_1706_cov_4.243489, whole genome shotgun sequence.
ACCESSION   DAOMPG010000342 DAOMPG010000000
VERSION     DAOMPG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26528_length_1706_cov_4.243489"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLS34_12830"
     CDS             complement(<1..543)
                     /locus_tag="PLS34_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88385.1"
                     /translation="MSLSARLLLPCLLCLAGLAQAAPPQFEATGFGAYRLGGEFDVDN
                     PPAGAPDSADLEDGGGWGAGLALYRDPDSFYEFLYSRQSTRIDRNAPVVGSLEVTTEY
                     YHLGGSLLFDARPALRSWLSLTVGVTRFAADGYSAETEFSASLGGGVRMPLGERVALT
                     LGLRGYLTFVDTDTQFFCSSV"
     gene            585..1211
                     /locus_tag="PLS34_12835"
     CDS             585..1211
                     /locus_tag="PLS34_12835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRP88386.1"
                     /translation="MTRLSHKFCQRDPVRLARALLGQRLVRVLDDGTRLAGRIVETEA
                     YLGVVDRAAHSYGGRRTARNAAMWGPAGRAYVYFVYGLHHCMNVVAEGVGQPTAVLIR
                     ALEPLEGLERMRVHRAGKRAADRLRDTDLCSGPAKLAQALALDRALDGADLVAGDTLF
                     LERGAAVAPRRIVAAPRIGVAYAAEWADQPLRFYVAGNPHVSTTARRD"
     gene            1245..1565
                     /locus_tag="PLS34_12840"
     CDS             1245..1565
                     /locus_tag="PLS34_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012154189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP88387.1"
                     /translation="METAAAASRLASLGHELRLELFRALVQAGDEGVAVGELQAMVDR
                     PASTVAFHLRELVDAGLVRQHKEGRVIRCHANFGALNELLLYLKHNCCGGVSLPVFRR
                     AGAS"
BASE COUNT          254 a          569 c          627 g          256 t
ORIGIN      
        1 gacgctgctg cagaagaact gcgtgtccgt gtcgacgaaa gtcaggtagc cgcgcaggcc
       61 cagtgtcagc gcaacccgct cgccgagcgg catgcgcacg ccgccaccga ggctggcgga
      121 gaactcagtc tcggcgctgt agccgtccgc ggcaaagcgc gtcacgccga cggtcagcga
      181 cagccagctg cggagcgcgg gccgtgcgtc gaacagcagg ctgccgccga ggtggtagta
      241 ctcggtggtg acttccagcg acccgaccac gggcgcattg cggtcgatcc gggtggattg
      301 cctggaatac aggaactcgt agaaggaatc gggatcgcgg tagagcgcca ggcccgcgcc
      361 ccagccgccg ccgtcctcga ggtcggcgct gtccggggcg ccggccggcg ggttgtcgac
      421 gtcgaactcg ccgccgaggc ggtaggcgcc gaagccggtg gcctcgaact gcggcggcgc
      481 ggcctgcgcg aggccggcga ggcatagcag gcagggcaac aggagccggg ccgacaacga
      541 catgggcgat ccttctgacg ccgggacagg gttagcataa acccgtgacc cggttgagcc
      601 acaagttctg ccagcgcgat cccgtgcgcc tggcccgcgc cctgctcggg cagcggctgg
      661 tgcgggtgct cgatgacggc acccgcctgg cgggccggat cgtcgagacc gaggcctacc
      721 tgggggtcgt cgatcgcgcc gcgcacagct acggcggccg tcgcacggcg cgcaatgccg
      781 cgatgtgggg gccggccggg cgcgcctacg tgtacttcgt ctacggcctg caccattgca
      841 tgaacgtggt ggccgagggc gtcgggcagc cgacggccgt gctgatccgg gcgctcgagc
      901 cgctcgaggg cctcgagcga atgcgcgtcc accgggccgg caagcgggcg gcggaccgcc
      961 tgcgggacac cgacctgtgc tccgggccgg ccaagctggc ccaggcgctg gcgctggacc
     1021 gcgcgctgga cggcgcggac ctggtggcgg gagatacgct gttcctggag cgcggtgcgg
     1081 cggttgcgcc gcgacgcatc gtggctgcgc cgcgcatcgg cgtggcctat gcggcggaat
     1141 gggccgacca gccgctgcgc ttctacgtgg ccggcaaccc gcacgtcagc accaccgccc
     1201 ggcgtgattg acaaaactat ttttctagtt atattgtccg acccatggaa accgccgccg
     1261 ccgccagccg tctcgccagc ctcgggcatg agctgcgcct cgagctgttc cgcgcgctgg
     1321 tgcaggcggg cgacgagggc gtggcggtcg gcgaactgca ggccatggtg gaccggccgg
     1381 cctcgacggt ggccttccac ctgcgcgagc tggtcgacgc cgggctggtc cggcagcaca
     1441 aggaaggccg cgtgatccgc tgccatgcga atttcggcgc actgaacgaa ttgctgctct
     1501 acctgaaaca caactgctgc ggcggcgtgt ccctgccggt gtttcgccgg gcgggcgcga
     1561 gctgaggtgg gccgtttttt tggctcgtta ataactactt acttaggaat atagatatgt
     1621 tgacgcccaa tgaagcccgg ccggccccgg tttgtggatg cgccgccccg atgcccgacg
     1681 ccggtcattt gcggcgcgca ctgcgc
//
LOCUS       DAOMPG010000343         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_26608_length_1703_cov_3.344053, whole genome shotgun sequence.
ACCESSION   DAOMPG010000343 DAOMPG010000000
VERSION     DAOMPG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26608_length_1703_cov_3.344053"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLS34_12845"
     CDS             <1..649
                     /locus_tag="PLS34_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP88388.1"
                     /translation="PLLDLIEEGNLGLIHAVKKFDPEKGFRFSTYATWWIRQTIERAI
                     MNQTRTIRLPIHVVKEINIYLRAARRLTQELDREPSAEEIAELLDREPLDVNRMLGLN
                     ERHVTSGHIVGRDGERNLLDTIPDVDEADPSYQAQADDIHEVLDHWLERLTDKQRAVV
                     ERRFGLHGHERATLEQVGEQIGVTRERVRQIQMDALARLRSIMESHGLSQDALLD"
     gene            complement(656..1078)
                     /locus_tag="PLS34_12850"
     CDS             complement(656..1078)
                     /locus_tag="PLS34_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HRP88389.1"
                     /translation="MICSATCRKGEFHYLTSMRFTLEMPPGTNLVTGVGPHGVRVGEQ
                     WHARSLIVSAAELVTDWPVANAASLDLELLAPALQLAPEILLLGTGSRIHFPDGALFA
                     ALAARNIGLEVMDTAAACRTYNVLVNEHRPVVAALILP"
     gene            1079..>1703
                     /locus_tag="PLS34_12855"
     CDS             1079..>1703
                     /locus_tag="PLS34_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88390.1"
                     /translation="MFTLFLPGIESAPEGASPRLPALERLLTRGRARPLATTPWALLA
                     RLAGGATDRWPVGPVSALGDGVGAPQACLRVEPLGSQQEQGGAFRLPAAQLAITQDEA
                     AALAGAFHDAFGADGWRLVIAVPERWYLVAQDARASAAGWSGFAGPARLPGDGERPAP
                     PEPGLRRLLSEVEMLFHAHPVNAARRARGTALIAGLHPWGGGRLAAGA"
BASE COUNT          250 a          602 c          626 g          225 t
ORIGIN      
        1 gccgctgctg gacctgatcg aagaaggcaa cctcggcctg attcacgcgg tcaagaagtt
       61 cgatcccgag aagggctttc gcttctcgac ctacgccacc tggtggatcc gccagaccat
      121 cgagcgggcg atcatgaacc agacccgcac catccggctg ccgatccatg tcgtgaagga
      181 gatcaacatc tacctgcgcg cggcccggcg cctgacgcag gagctggacc gcgagccttc
      241 ggccgaggag atcgccgagc tgctggaccg cgagccgctc gacgtgaacc gcatgctcgg
      301 gctcaacgaa cggcacgtca ccagcggcca catcgtcggc cgggacggcg agcgcaacct
      361 gctggacacc atccccgacg tggacgaggc ggatccttcc taccaggcgc aggccgacga
      421 catccacgag gtgctcgacc actggctgga gcggctgacc gacaagcagc gggcggtggt
      481 cgagcgacgc ttcggcctgc acggccacga gcgcgccacg ctcgagcagg tcggcgagca
      541 gattggcgtc acgcgcgagc gcgtccgcca gatccagatg gacgcgctcg cccggctgcg
      601 cagcatcatg gagagccacg ggctctcgca ggacgcgctg ctggactgat tcggctcagg
      661 gcaggatcag cgccgccacc accggccggt gctcgttgac cagcacgttg taggtgcgac
      721 aggcggcggc ggtgtccatc acctccaggc cgatgttgcg cgccgcgagc gcggcgaaca
      781 gcgcgccgtc cgggaagtga atgcggctgc cggtcccgag cagcaggatc tccggcgcca
      841 gctgcagcgc cggcgccagc agctcgagat cgaggctcgc ggcgttcgcc accggccagt
      901 ccgtcaccag ctcggccgcg ctgacgatca ggctgcgcgc atgccactgc tcgccgacgc
      961 ggacgccgtg cgggccgacg ccggtgacga ggttcgtgcc gggaggcatc tcgagcgtga
     1021 agcgcatgga agtaagataa tgaaattccc ccttgcgaca agtcgcagag cagatcacgt
     1081 gttcaccctg ttcctgcccg gcatcgagtc ggcccccgag ggcgcgtcgc cgcgtctgcc
     1141 cgcgctggag cgcctgctca cgcgtggccg tgcccgcccg ctcgcgacga cgccctgggc
     1201 gctgctcgcg cggctcgctg gcggcgcgac cgaccgctgg ccggtcgggc cggtcagcgc
     1261 gctcggtgac ggggtcggcg cgccgcaggc ctgcctgcgg gtcgagccac tcgggtcgca
     1321 gcaggagcag ggcggggcat tccggctgcc cgcggcacag ctggcgatca cgcaggacga
     1381 agccgcggcg ctggccgggg cgtttcacga cgccttcggc gcggacggct ggcgactggt
     1441 gatcgccgtg ccggaacgct ggtacctggt ggcgcaggac gcgcgcgcga gtgcggccgg
     1501 ctggtcgggc ttcgccggtc cggcgcggct gcccggcgac ggcgagcggc cggcgccgcc
     1561 cgagcccggg ctgcgcaggc tgctgtccga ggtcgagatg ctattccatg cccatccggt
     1621 gaatgcggcg cggcgcgcgc gcggcacggc gctcatcgcc ggcctgcacc cctggggggg
     1681 cggtcggctg gcggcggggg cgc
//
LOCUS       DAOMPG010000344         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate AMR_MDS_5866
            NODE_30307_length_1531_cov_3.494580, whole genome shotgun sequence.
ACCESSION   DAOMPG010000344 DAOMPG010000000
VERSION     DAOMPG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868087
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.267
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:22:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,575
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,512
            CDSs (with protein)               :: 2,512
            Genes (RNA)                       :: 38
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 11 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30307_length_1531_cov_3.494580"
                     /isolate="AMR_MDS_5866"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLS34_12860"
     CDS             <1..200
                     /locus_tag="PLS34_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP88391.1"
                     /translation="GARDASAGQPLARHAPGRFAVDDPDGLARIDRENLQYDSHQYFW
                     ELRSPIARVCSPAGHSQGVAA"
     gene            complement(223..414)
                     /locus_tag="PLS34_12865"
     CDS             complement(223..414)
                     /locus_tag="PLS34_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017022284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HRP88392.1"
                     /translation="MEALQTVLLQCPNCWEQVEIVVDYEDAEQEIVEDCSVCCSPMVI
                     ALTAGDDDELHAAARLENG"
     gene            complement(494..973)
                     /locus_tag="PLS34_12870"
     CDS             complement(494..973)
                     /locus_tag="PLS34_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRP88393.1"
                     /translation="MSHQDFELQVHWERGEQPFLDGRYSRRHRLRFDGELEVPGSSSP
                     QVVPVPLSDPAAMDPEEAFVASLSACHMLWFLAMASKYGFCVDEYRDTPLGRMGRNAD
                     GRTAMTLVLLRPAVRFSGARRPSPEEFRHMHHEAHEACFIANSVLTELQCDPVYLPG"
     gene            complement(1026..>1531)
                     /locus_tag="PLS34_12875"
     CDS             complement(1026..>1531)
                     /locus_tag="PLS34_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRP88394.1"
                     /translation="ARICADQDMHILFVPFLTDTQSAYMRVRICAKARAVENECYVAI
                     AGSVGNLPQVHNMDVQYGQSAIFTPADFAFPTNSIKAETTPNTEMTVIADLDMDLLKE
                     LHEIGSVTNLKDRRKDLYDIIVKAPLADSDLEAERRRGEIARVVADQDAADLDEAGQD
                     TAGKQAG"
BASE COUNT          235 a          517 c          517 g          262 t
ORIGIN      
        1 acggcgcgcg cgacgcatcc gcggggcagc cgctggcgcg tcacgcgccg ggccgcttcg
       61 cggtggacga cccggacggg ctggcgcgca tcgaccggga gaacctgcag tacgacagtc
      121 accagtactt ctgggagctg cgttcgccca tcgcccgggt ctgctcgccg gccgggcatt
      181 cgcagggcgt ggcggcctga agcggaacgc tagccgttct ccctagccgt tttccagccg
      241 cgccgcagca tgcagctcgt cgtcgtcgcc cgccgtcagg gcaatgacca tcgggctgca
      301 gcagacgctg cagtcctcga cgatctcctg ctccgcatcc tcgtaatcga cgacgatctc
      361 gacctgttcc cagcagttcg ggcactgcaa caatactgtc tggagggctt ccatggttga
      421 tagtgcctcg gtcatgaggc gccggacggc gctaccctga atgtagacca tcggaagcca
      481 gagtcgagct tcgtcagccc ggcaggtaca ccggatcgca ctgcagctcg gtgagcaccg
      541 agttggcgat gaaacaggcc tcgtgtgctt cgtggtgcat gtgccggaac tcttccggcg
      601 atggccgccg cgcgccggaa aagcggacgg ccgggcgcag caggaccagg gtcatcgcgg
      661 tccggccatc ggcgttcctg cccatcctgc ccagcggcgt gtcccggtac tcgtccacgc
      721 agaagccgta cttgctggcc atggccagga accacagcat gtgacaggcg gacagggagg
      781 ccacgaaggc ctcctcggga tccatcgcgg ccgggtccga cagcggtacc ggcacgacct
      841 ggggggagga cgagcccggc acctccagct cgccgtcgaa gcgcagccgg tgcctgcggc
      901 tgtaacgacc gtcgagaaac ggctgctcgc cgcgctccca gtggacctgc agctcgaaat
      961 cctggtggct catgcctgtt cctcccggca aaccgcgacg gcggcggcgg tcggccaccg
     1021 gccgttcagc cggcctgctt gccagccgtg tcctgcccgg cctcgtcgag gtcggccgca
     1081 tcctggtcag ccaccaccct ggcgatctcg ccgcggcgcc gctccgcctc gagatcgctg
     1141 tcggccagtg gcgccttgac gatgatgtcg tagagatcct tgcggcgatc cttcaggttg
     1201 gtgacgctgc cgatctcgtg cagctccttc agcaggtcca tgtcgaggtc ggcgatcacc
     1261 gtcatctcgg tgttcggcgt ggtctcggcc ttgatggaat tggtggggaa ggcgaaatcg
     1321 gcgggcgtga agatggccga ctggccgtac tgcacgtcca tgttgtgcac ctgcggcagg
     1381 ttgccgaccg agccggcgat ggccacgtag cactcgttct ccaccgcccg cgccttggcg
     1441 cagatcctga cgcgcatgta ggcgctctgg gtgtcggtga ggaagggcac gaacaggatg
     1501 tgcatgtcct ggtcggcgca gatgcgcgcc a
//
